LOCUS       DACDNO010000001       104546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDNO010000001 DACDNO010000000
VERSION     DACDNO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 104546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.001"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(258..533)
                     /locus_tag="IWI22_000001"
     CDS             complement(258..533)
                     /locus_tag="IWI22_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167429.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            827..1642
                     /gene="thiD"
                     /locus_tag="IWI22_000002"
     CDS             827..1642
                     /gene="thiD"
                     /locus_tag="IWI22_000002"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9167430.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1971..3422)
                     /gene="cls"
                     /locus_tag="IWI22_000003"
     CDS             complement(1971..3422)
                     /gene="cls"
                     /locus_tag="IWI22_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9167431.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3763..6429
                     /locus_tag="IWI22_000004"
     CDS             3763..6429
                     /locus_tag="IWI22_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9167432.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6793..7545
                     /locus_tag="IWI22_000005"
     CDS             6793..7545
                     /locus_tag="IWI22_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9167433.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7567..8721)
                     /locus_tag="IWI22_000006"
     CDS             complement(7567..8721)
                     /locus_tag="IWI22_000006"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9167434.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8906..11347
                     /locus_tag="IWI22_000007"
     CDS             8906..11347
                     /locus_tag="IWI22_000007"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9167435.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11677..12381)
                     /locus_tag="IWI22_000008"
     CDS             complement(11677..12381)
                     /locus_tag="IWI22_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167436.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12568..12813)
                     /locus_tag="IWI22_000009"
     CDS             complement(12568..12813)
                     /locus_tag="IWI22_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167437.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12830..13681)
                     /locus_tag="IWI22_000010"
     CDS             complement(12830..13681)
                     /locus_tag="IWI22_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9167438.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13847..14851
                     /locus_tag="IWI22_000011"
     CDS             13847..14851
                     /locus_tag="IWI22_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9167439.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(15092..15448)
                     /locus_tag="IWI22_000012"
     CDS             complement(15092..15448)
                     /locus_tag="IWI22_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9167440.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15816..17003
                     /gene="tuf"
                     /locus_tag="IWI22_000013"
     CDS             15816..17003
                     /gene="tuf"
                     /locus_tag="IWI22_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9167441.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLTE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(17191..19782)
                     /gene="mprF"
                     /locus_tag="IWI22_000014"
     CDS             complement(17191..19782)
                     /gene="mprF"
                     /locus_tag="IWI22_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9167442.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19808..20527)
                     /locus_tag="IWI22_000015"
     CDS             complement(19808..20527)
                     /locus_tag="IWI22_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9167443.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            20736..21368
                     /locus_tag="IWI22_000016"
     CDS             20736..21368
                     /locus_tag="IWI22_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9167444.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            21427..22287
                     /locus_tag="IWI22_000017"
     CDS             21427..22287
                     /locus_tag="IWI22_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9167445.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            22394..24787
                     /locus_tag="IWI22_000018"
     CDS             22394..24787
                     /locus_tag="IWI22_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9167446.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24969..26810
                     /locus_tag="IWI22_000019"
     CDS             24969..26810
                     /locus_tag="IWI22_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9167447.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26834..27943
                     /gene="rpoD"
                     /locus_tag="IWI22_000020"
     CDS             26834..27943
                     /gene="rpoD"
                     /locus_tag="IWI22_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9167448.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            28163..30262
                     /locus_tag="IWI22_000021"
     CDS             28163..30262
                     /locus_tag="IWI22_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9167449.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            30458..31321
                     /locus_tag="IWI22_000022"
     CDS             30458..31321
                     /locus_tag="IWI22_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9167450.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            31426..31899
                     /locus_tag="IWI22_000023"
     CDS             31426..31899
                     /locus_tag="IWI22_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9167451.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            31995..32882
                     /gene="xerD"
                     /locus_tag="IWI22_000024"
     CDS             31995..32882
                     /gene="xerD"
                     /locus_tag="IWI22_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9167452.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            32901..33272
                     /locus_tag="IWI22_000025"
     CDS             32901..33272
                     /locus_tag="IWI22_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9167453.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            33242..34039
                     /locus_tag="IWI22_000026"
     CDS             33242..34039
                     /locus_tag="IWI22_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9167454.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            34023..34592
                     /gene="scpB"
                     /locus_tag="IWI22_000027"
     CDS             34023..34592
                     /gene="scpB"
                     /locus_tag="IWI22_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9167455.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            34598..35314
                     /locus_tag="IWI22_000028"
     CDS             34598..35314
                     /locus_tag="IWI22_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9167456.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      35396..35521
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            35644..36255
                     /locus_tag="IWI22_000029"
     CDS             35644..36255
                     /locus_tag="IWI22_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9167457.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            36633..38156
                     /locus_tag="IWI22_000030"
     CDS             36633..38156
                     /locus_tag="IWI22_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9167458.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            38393..39733
                     /locus_tag="IWI22_000031"
     CDS             38393..39733
                     /locus_tag="IWI22_000031"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9167459.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            39886..40623
                     /locus_tag="IWI22_000032"
     CDS             39886..40623
                     /locus_tag="IWI22_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9167460.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(40788..41009)
                     /locus_tag="IWI22_000033"
     CDS             complement(40788..41009)
                     /locus_tag="IWI22_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9167461.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            41040..42071
                     /locus_tag="IWI22_000034"
     CDS             41040..42071
                     /locus_tag="IWI22_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167462.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            42058..43461
                     /locus_tag="IWI22_000035"
     CDS             42058..43461
                     /locus_tag="IWI22_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9167463.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            43527..44162
                     /locus_tag="IWI22_000036"
     CDS             43527..44162
                     /locus_tag="IWI22_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9167464.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            44211..44891
                     /locus_tag="IWI22_000037"
     CDS             44211..44891
                     /locus_tag="IWI22_000037"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9167465.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            45026..46255
                     /gene="rpsA"
                     /locus_tag="IWI22_000038"
     CDS             45026..46255
                     /gene="rpsA"
                     /locus_tag="IWI22_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9167466.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            46437..47747
                     /locus_tag="IWI22_000039"
     CDS             46437..47747
                     /locus_tag="IWI22_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9167467.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            47990..48265
                     /locus_tag="IWI22_000040"
     CDS             47990..48265
                     /locus_tag="IWI22_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9167468.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            48500..49546
                     /locus_tag="IWI22_000041"
     CDS             48500..49546
                     /locus_tag="IWI22_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167469.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            49548..50807
                     /locus_tag="IWI22_000042"
     CDS             49548..50807
                     /locus_tag="IWI22_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9167470.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            50813..51394
                     /locus_tag="IWI22_000043"
     CDS             50813..51394
                     /locus_tag="IWI22_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9167471.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(51476..52351)
                     /locus_tag="IWI22_000044"
     CDS             complement(51476..52351)
                     /locus_tag="IWI22_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9167472.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            52499..52831
                     /locus_tag="IWI22_000045"
     CDS             52499..52831
                     /locus_tag="IWI22_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9167473.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52943..54151
                     /locus_tag="IWI22_000046"
     CDS             52943..54151
                     /locus_tag="IWI22_000046"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9167474.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            54305..54760
                     /locus_tag="IWI22_000047"
     CDS             54305..54760
                     /locus_tag="IWI22_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167475.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            55122..55397
                     /locus_tag="IWI22_000048"
     CDS             55122..55397
                     /locus_tag="IWI22_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167476.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            55410..57488
                     /locus_tag="IWI22_000049"
     CDS             55410..57488
                     /locus_tag="IWI22_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9167477.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            57648..59534
                     /locus_tag="IWI22_000050"
     CDS             57648..59534
                     /locus_tag="IWI22_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9167478.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            59546..60493
                     /locus_tag="IWI22_000051"
     CDS             59546..60493
                     /locus_tag="IWI22_000051"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9167479.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            60513..61040
                     /locus_tag="IWI22_000052"
     CDS             60513..61040
                     /locus_tag="IWI22_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9167480.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(61103..61756)
                     /locus_tag="IWI22_000053"
     CDS             complement(61103..61756)
                     /locus_tag="IWI22_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9167481.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            61888..62730
                     /locus_tag="IWI22_000054"
     CDS             61888..62730
                     /locus_tag="IWI22_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9167482.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            62888..63783
                     /locus_tag="IWI22_000055"
                     /pseudo
     CDS             62888..63783
                     /locus_tag="IWI22_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
     gene            63786..64499
                     /locus_tag="IWI22_000056"
     CDS             63786..64499
                     /locus_tag="IWI22_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9167483.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            64519..65037
                     /gene="msrA"
                     /locus_tag="IWI22_000057"
     CDS             64519..65037
                     /gene="msrA"
                     /locus_tag="IWI22_000057"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9167484.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            65034..65261
                     /locus_tag="IWI22_000058"
     CDS             65034..65261
                     /locus_tag="IWI22_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9167485.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            65413..65964
                     /gene="lepB"
                     /locus_tag="IWI22_000059"
     CDS             65413..65964
                     /gene="lepB"
                     /locus_tag="IWI22_000059"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9167486.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(66047..66589)
                     /locus_tag="IWI22_000060"
     CDS             complement(66047..66589)
                     /locus_tag="IWI22_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9167487.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(66826..67224)
                     /locus_tag="IWI22_000061"
     CDS             complement(66826..67224)
                     /locus_tag="IWI22_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9167488.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            67484..68344
                     /gene="ylqF"
                     /locus_tag="IWI22_000062"
     CDS             67484..68344
                     /gene="ylqF"
                     /locus_tag="IWI22_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9167489.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            68337..69104
                     /locus_tag="IWI22_000063"
     CDS             68337..69104
                     /locus_tag="IWI22_000063"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9167490.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            69160..70017
                     /gene="dprA"
                     /locus_tag="IWI22_000064"
     CDS             69160..70017
                     /gene="dprA"
                     /locus_tag="IWI22_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9167491.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            70139..72217
                     /gene="topA"
                     /locus_tag="IWI22_000065"
     CDS             70139..72217
                     /gene="topA"
                     /locus_tag="IWI22_000065"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9167492.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            72186..73562
                     /gene="trmFO"
                     /locus_tag="IWI22_000066"
     CDS             72186..73562
                     /gene="trmFO"
                     /locus_tag="IWI22_000066"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9167493.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            73760..74665
                     /gene="xerC"
                     /locus_tag="IWI22_000067"
     CDS             73760..74665
                     /gene="xerC"
                     /locus_tag="IWI22_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9167494.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            74702..75250
                     /gene="hslV"
                     /locus_tag="IWI22_000068"
     CDS             74702..75250
                     /gene="hslV"
                     /locus_tag="IWI22_000068"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9167495.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            75264..76664
                     /gene="hslU"
                     /locus_tag="IWI22_000069"
     CDS             75264..76664
                     /gene="hslU"
                     /locus_tag="IWI22_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9167496.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            76682..77473
                     /gene="codY"
                     /locus_tag="IWI22_000070"
     CDS             76682..77473
                     /gene="codY"
                     /locus_tag="IWI22_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9167497.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            77555..78430
                     /locus_tag="IWI22_000071"
     CDS             77555..78430
                     /locus_tag="IWI22_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9167498.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(78562..79200)
                     /gene="plsY"
                     /locus_tag="IWI22_000072"
     CDS             complement(78562..79200)
                     /gene="plsY"
                     /locus_tag="IWI22_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9167499.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            79327..79761
                     /locus_tag="IWI22_000073"
     CDS             79327..79761
                     /locus_tag="IWI22_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9167500.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            79927..81972
                     /gene="parE"
                     /locus_tag="IWI22_000074"
     CDS             79927..81972
                     /gene="parE"
                     /locus_tag="IWI22_000074"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9167501.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            81984..84434
                     /gene="parC"
                     /locus_tag="IWI22_000075"
     CDS             81984..84434
                     /gene="parC"
                     /locus_tag="IWI22_000075"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9167502.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            84711..86942
                     /gene="pflB"
                     /locus_tag="IWI22_000076"
     CDS             84711..86942
                     /gene="pflB"
                     /locus_tag="IWI22_000076"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9167503.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTGWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            87186..87947
                     /gene="pflA"
                     /locus_tag="IWI22_000077"
     CDS             87186..87947
                     /gene="pflA"
                     /locus_tag="IWI22_000077"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9167504.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            88032..88958
                     /locus_tag="IWI22_000078"
     CDS             88032..88958
                     /locus_tag="IWI22_000078"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9167505.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            89140..90033
                     /locus_tag="IWI22_000079"
     CDS             89140..90033
                     /locus_tag="IWI22_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9167506.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            90167..90292
                     /locus_tag="IWI22_000080"
     CDS             90167..90292
                     /locus_tag="IWI22_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9167507.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(90426..91058)
                     /locus_tag="IWI22_000081"
     CDS             complement(90426..91058)
                     /locus_tag="IWI22_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167508.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(91206..91748)
                     /locus_tag="IWI22_000082"
     CDS             complement(91206..91748)
                     /locus_tag="IWI22_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9167509.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            91883..92272
                     /locus_tag="IWI22_000083"
     CDS             91883..92272
                     /locus_tag="IWI22_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167510.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            92276..93040
                     /locus_tag="IWI22_000084"
     CDS             92276..93040
                     /locus_tag="IWI22_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9167511.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            93169..94116
                     /locus_tag="IWI22_000085"
     CDS             93169..94116
                     /locus_tag="IWI22_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9167512.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            94246..94986
                     /locus_tag="IWI22_000086"
     CDS             94246..94986
                     /locus_tag="IWI22_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167513.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(95277..95945)
                     /locus_tag="IWI22_000087"
     CDS             complement(95277..95945)
                     /locus_tag="IWI22_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9167514.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            96079..96366
                     /locus_tag="IWI22_000088"
     CDS             96079..96366
                     /locus_tag="IWI22_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167515.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            96782..98320
                     /locus_tag="IWI22_000089"
     CDS             96782..98320
                     /locus_tag="IWI22_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9167516.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            98317..99282
                     /locus_tag="IWI22_000090"
     CDS             98317..99282
                     /locus_tag="IWI22_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167517.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            99269..100591
                     /locus_tag="IWI22_000091"
     CDS             99269..100591
                     /locus_tag="IWI22_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9167518.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      100654..100758
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            100832..101575
                     /locus_tag="IWI22_000092"
     CDS             100832..101575
                     /locus_tag="IWI22_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167519.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            101590..102582
                     /locus_tag="IWI22_000093"
     CDS             101590..102582
                     /locus_tag="IWI22_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9167520.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            102594..103331
                     /locus_tag="IWI22_000094"
     CDS             102594..103331
                     /locus_tag="IWI22_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167521.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            103321..104043
                     /locus_tag="IWI22_000095"
     CDS             103321..104043
                     /locus_tag="IWI22_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9167522.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(104127..>104546)
                     /locus_tag="IWI22_000096"
     CDS             complement(104127..>104546)
                     /locus_tag="IWI22_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9167523.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        35463 a        17980 c        21936 g        29167 t
ORIGIN      
        1 agccatttgg cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct
       61 aacaatttga agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg
      121 tgtttttact agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg
      181 cattcggttt tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
      241 taaagaacag ttagaagtta aaattgctga ttcacataga gtttatgact tcttggaaat
      301 tctttttcaa tcagtttttt catctgttta tgaaattctt ttttttcagt aaacataaag
      361 gtttgggtat cgtaaccttc ttcatgtagg aaatagatta ttcctttttc tgtgttttgc
      421 gtataagctt ttatactcct gtcttttttg aaagaacaga tcgtatagcc atctatttgg
      481 tctggtagtc gtttgatttt cttcaaaatg acttttttat cctcaagttt catttttttc
      541 acctgacttt atttttttca tatggaaatc atagcgcctt tggttatatg aaaacaagaa
      601 ggatactgaa atatatataa atcaaatagt ggttatttat tattagaata gcgaatacaa
      661 ataaacgacc actattttct gatttattgc aaatgttttt tcaaataaat ttgattacca
      721 agaaaaggta gtggtatatt tactgaggga tactttgaaa aacatatgat agaaacagta
      781 acagtattat gaaaaatctt tttataaaaa tggaggaatc gatttgatgg aagatcagta
      841 tgtacaagca atgacgatcg caggatcaga tagcgatgga agcgcaggga tgcaagcaga
      901 tatgcataca ttctttacaa gaaatgttta cggtgtttct gtcatgaccg catgtgttgc
      961 aggtaattct tacggaatcg gagcaagtgt gacattgcca acagatttta tcgacaaaga
     1021 atttgaactg attgcgaagg actttcaaat acgtgcagct aaaacaggta tgctagcaga
     1081 ttcaaagttg attgagacag ttgtaaaaaa ttaccaaaaa tatgattttg gaccattagt
     1141 cgtggatccc gtaatcgtaa cgaaacatgg aaatctttta ctagaagaat ctgctttgca
     1201 aagcttgaaa gaaaagcttg tccctttggc cgaagtactg acacctaatt tttatgaagc
     1261 agaaaagctg actggacgga cattagcagc tgaagaagat tttgtgcaag cagcaaaaga
     1321 actgcaagat atgggtgcaa aaaacgttat gatcaaagga catcatgcta aaaatgcgca
     1381 gcaagaaaca gtcaatgact acgtattgtt cgaatcagga aaatcatttt ggttaaatgc
     1441 accattttat cctaccgacc ggatcaacgg aactggcgat tcgctatctg catgtattgc
     1501 agctgagtta gcgaaaggaa aaacagttga acaagcgatc cgattggcaa aagactatgt
     1561 aaaccgtgca attcgtgaag aaataaatgt agggcacaaa tatggcccca tcaatcattg
     1621 ggcagctggt gaattagagt aaaagaaaga agttgtgaaa aagttgccct gcatcaagta
     1681 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggttgga cttattaccg
     1741 cagatgcaag cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tccagctccg
     1801 agtcacaagg aacaagttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg
     1861 accgaggagt tggcaggaga acaccgtttg ttcggaagaa gggactgtga caagattttt
     1921 gtcacagtcc ctttaatctt agtatatttt tctctacttg aataaaaaaa ttataggatt
     1981 ggagaaaata atcgagaaat ttgttgcttg aataatagcc agttagacat ttccttgact
     2041 gtttcagttg tcatggatga acaatgtgtc tcatcttctt tgaactgagt agcaagttct
     2101 tctaaaaact caggatcgta aatgacagca cttgtttcaa aattcaatcg gtagctacga
     2161 atatcttgat ttgctgaacc taccatgcag atttcatcat ccattatcaa tgtttttgca
     2221 tggaggaagc cgttctcata gacaaggatt tgtacatttt ccttgatcag ctgccgagca
     2281 taatattgtg ttgctcgata aataaaagga tgatccggtt tgttgggaat catgattttc
     2341 acatctacac cagaggcagt tgcgattttt aaagcagcaa tgacactttc atcaggaaca
     2401 aggtaaggtg tctgtatcca aacacgtttt ttagcagaag taatcaattt gataaatgaa
     2461 agcttgattt gttccctttc gttattcggt ccgcttgaaa caagctggat ggatgtatca
     2521 gcaagcttag attcatctcg atctgctttt ttaaagaaat aattcaaatg atagccgacc
     2581 tttttttctt caggtaccga gacgttccag tctgttaaga agcgaagctg gagcaaagaa
     2641 gaagcggcac cgaaaatccg tatatgcgta tcgcgccaat agccgaattt ttttgttgta
     2701 tttacatatt gattggcaat attgaaacca ccggtataac taatctttcc atcgatcaca
     2761 acgatttttc ggtgatcatg gtaattcaat cggaaacgaa gtaatgccct ttgtgaggta
     2821 acaaatgtat ggacatgtcc accatttttg attagacgat tgaaatcttt tgcttttgtg
     2881 ccatgagagc caaatgcatc atataatatt cgaacttcca cgccttctgc ggctttttct
     2941 tctaaaacat gtaagacttg ctggccgata ttatctgtca caaatgcgta atattcgata
     3001 tggatcgaat gtttggcttt ttttagatct tgaagcagtg aatccaattt ctcttgtccg
     3061 tctgtgagaa gagtgacaga attcattctt gtcagcggca tacgatttaa tgaagagaag
     3121 aagtcaacaa attgctgttt gtctttatcg cccatgtcag gatcgtaatg ttcaatggta
     3181 tctcctctaa aagactgaaa gttttctaat tctttcaagt cactttgtcg gagatagaat
     3241 tttttcttat ccgttaatcc acgtccgaaa aataaatata atacaaagcc caccccagga
     3301 agggcaaaca atactaagag ccatgcccaa atggctgcta catctcgggg tttgatcaaa
     3361 atagccacca aagcaataag tgcatttaat agataaaggg cggttataat actagatacc
     3421 acaaaaatgt tcctccttac atacaataac attagtatag catgatagaa gaaagaagcc
     3481 taccaaggtg aatatacttt tcactccttt catgaaaatg taggtaaatc gggctaaagt
     3541 agttgctttt tgtaggttaa taaggtagga tattagtaca gttgaatatc tccttagggg
     3601 agtaaccggc aacgtttgtt gcgataatat caacagtaag ttaaccaaat ggttttctgg
     3661 tattgtatga aatggtgaga cctaagacaa atgcgtattt gtcttaggtc tctttttttt
     3721 cagacaggac tagggccaag atcaaaaaat gggggatgaa atttggaaaa caaggaacaa
     3781 gcacaacaag cggtctataa agaaacgatc gactcgttga tgcagcaaac gcagagctca
     3841 accgatggat tgagcaccac gcaagcgaag gaaaaattag aaagagatgg cttgaaccag
     3901 ctaaaagaaa agccgaagaa gccaacttgg aaattgttct tagaaacttt caaagatgca
     3961 atggttatcg tattgctgat tgtcgcactg attcagatgg tcatgggtga ctacatcgaa
     4021 ttcttagtta tttttgcagt attgatgttg aattctataa tcagtgttgt ccaaacaaaa
     4081 aaagcagaag gttctttaga agcgttgaaa aacctttcag cgcccgatgc tcgagtgatt
     4141 cgtgatggta aggaaaaaac gattccggca aacgaacttg tggtaggaga tatcgtgttg
     4201 ttagacgcag gcgattatgt acctgctgat ggacgtttac tagatgcggg atcattaaaa
     4261 gtagatgaag gtatgctgac aggagagtcg acttctgcag aaaaagaagt aacggatatc
     4321 acaagtacgg taccaatcgg agatcgtaca aatatggtat tcagtggaac gatcgtaact
     4381 tacggacgcg gtaaattctt agtgacttca actgggaata atacggagat cggacaagtt
     4441 gctggccttt tggaatcaac aactgagcaa gtcactccgt tacaacgagg tctggataaa
     4501 ttcagtaaaa aactaagttt ggctattttg gcattgtctt tattgatttt aggtatccaa
     4561 ctgtttcgta tttatcttgg agaaggtaca ggagatatga ctgcagatat cgtcagcgca
     4621 gtgatgtttg ccgtagccgt agccgtagct gctattccag aagcattaca gtctatcgta
     4681 acaatcgtgt tatctttggg aacgaataaa atggcaaaac gtcatgccat tattcggaag
     4741 cttccagcag ttgaaacact tggttcgaca agtatcatct gtacagataa gactggtaca
     4801 ttgacacaaa acaaaatgac agttgttgac tactttttag gtaatggcga tactggggat
     4861 ttcacaaatg acccttcaaa atggacagct gacgaacgtc gcttgatcga aatcagtgtt
     4921 ttagcgaatg atgcatcgat cagtgaagac ggtactaaat tgggcgaccc tacggaagta
     4981 gcgtttatcg actttagtga aaaattaaac cagccttatc aggaaatccg aaacaactac
     5041 ccaagacaag cagaacttcc ttttgactca gatcgaaaat taatgtctac tggacacaca
     5101 attgataaca aaaattactt gtttgttaaa ggcggaccag atatcgtctt ccaacgcagt
     5161 aaaaaagtat tgctaaatgg tgaagtcgtt ccgatgacgg atgaattgat gaaaaaattc
     5221 caagagcaaa atgaagcttt ttcaacacgc gcattacgtg tactagcttt tgcttaccgt
     5281 ccacttgata attttgattt atcatttgaa gatgaagacg atttgatcct tgttggtttg
     5341 cttgctatga tcgatcctcc acgtgctgaa gtgactcagg cggtaacaga agcaagaagt
     5401 gcaggaatca aaacaatcat gattactggt gaccacaaaa caacagctgt tgctatcgca
     5461 aaacaaatcg ggatttttga agaaggaaac ctggctttga ccgggacgga actagatgaa
     5521 ttgacagaag atcaactaaa cgaagtttta gaaaaagtca ctgtttatgc acgtgtttca
     5581 cctgagaata aaattcgtat cgttcgtgct tggcaaaata aaggtcatat ctcagctatg
     5641 acaggtgatg gtgtaaatga cgcaccagca ttgaaacaag cagacatcgg aattgctatg
     5701 ggaacgggta cagatgtggc aaaagatgca gcagcgatgg tattgacgga tgataacttt
     5761 gcttctattg tcagcgcagt agaagtagga cgtaatgtgt ttgataatat caagaaagct
     5821 atttcttatc tatttgcagg aaatttaggt gcgatcattg cgatcatctt tgccttagtt
     5881 gctgactggt ccaatccatt tacagcatta caactgttgt tcatcaattt agtcaatgat
     5941 tcactgccag caattgcatt aggtacagaa aaagctgaac ctgctacgat gaaccgcccg
     6001 ccaagagatc caaattcagg aatctttacg gacaaaacat tgatttctgt aacgtatcgt
     6061 ggtgtgctga ttgggattgc tgttatcttt gctcaatggc ttggcatgca gcattcacct
     6121 gaattcggtg tagccatgtc attttcaaca ttgatttggt gtcggacatt gcaaacattc
     6181 ccagcacgtt caaacacaca aacagctatc caagcagggt tcttctctaa cagaagtgtc
     6241 ttactagctg tagttgtatg tagcgcgctt tactgcttaa caatgatccc aggactgaaa
     6301 gaagtcttct ctatcccatc agcatttgga ttgacagaag tctttacatg tatgggaatc
     6361 gcacttgtga caattgtttt gatggaaatc actaaattga tcattgtacg agtacaagga
     6421 aataaataaa acgaatcaac gaggttgtga gcctgccgtt taattctgag tcacaaggtg
     6481 caatttcaac aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaagagttg
     6541 gcaggagaac accgtttaag gaagaagttg taaaatcagc attttgagct aatactgagg
     6601 gtcatctgat tggacactgt ttattcggaa agagggactt tgacacgact ttggtcacag
     6661 tccctttttt tatttatgat agacggaaag ccaatcattt tattattcat caaaagagat
     6721 agaatagata aaagaggaga atgatttatt ccgaaaagtg aaaacatata acaaaaaatt
     6781 agaggaaatc aaatgaatga aaaagaatgg gatgaatttg ctgaagaata ttatgaaatc
     6841 cagcaagaat cgcaagtaac tattgctgaa gatgtaaaaa attatctgaa aacggagaaa
     6901 gtcctgcctg ctgaaacagc agcagacgtt gctggaggat ctggcagatt tctttcctta
     6961 gcaaaagaaa ttaatcgcta tgatttgatc gatttttctt caggaatgct agaaaatgca
     7021 aaaaaagaag cagaaaaaaa tgagttgcca aatgtttcct ttatcaaaca gtcgttagaa
     7081 gaatttatcc aatcaaatga gacatatgaa atggttttta cagcagcaaa tccagcattg
     7141 gtacatctta ttcaattaga aaaattgatg aaaaagtcaa gcaaatggtg cgtgataatc
     7201 agagtagtgg aatcaatcga tgatgttttt tatcctatcg aaaagcaact aggaatgata
     7261 gaagaagatc ctaatacatc gccagttgta atggatcaat ttgaaacata cttaattgat
     7321 gaaggattca aagtgaagaa aaaagatttt acttatttta cacaggaatc aattactgaa
     7381 gaattgatga agctatacta tgacgcgtac gcaaaagatc ctgttgtgac caggtacatt
     7441 cgcaaactat tcagtgaaaa acgagaaatc cttagtacga ctaagctgac ctatagactg
     7501 ctcctttggt ccgcagataa aaaaagaacc tttcacgact cgtgaagggt tctttttatg
     7561 attttattat attttgtagt aacgaatatc attttcgatc tttgtaatcg aaaaaggtac
     7621 atctgaagta tattctgcac attctccgtg ttcaggaagc gtctcactga aaatcgtttc
     7681 atattcgtca acagtgatcc gtgttcgaga atctagcatc tcttgatgag attgtgcgaa
     7741 gagatattct tgatagtttt cttccagata acctgtaaaa aattcactta ctgcaccaga
     7801 accgtagctg aatagaccga tgcgatctcc tggctgtagt gatttcgaat tttccagtag
     7861 agaagttagc cccaggtata atgaaccagt gtaaagatta ccgattcgtc ggctgtaacg
     7921 gatgctttct tcatagcgag ccataagacg ttcctgattt tcttcgtctg tttggtctaa
     7981 tacgctttgc aatgcctttt ttcccatctt agtatacgga atatggaaag caatcgcctg
     8041 ataatcttcg agtcctcgac ccgacaattc tttatgtcga ttccaaactt tttggaatga
     8101 ttcgatatac gtagaattag ataaaggacc atcaacaaca ggaaattcgc tataatctgg
     8161 acgccagaaa tcatagatat cttctgtcag aaatacgctg tcgtcttcaa tcgataaaat
     8221 acgcgggttt tgagtgatca tcatcgcaac agcaccgaca ccttgcgtca cttcaccacc
     8281 gcttgccaag ccgtaacgag caatatcact tgctatgact agtacttttc gttctggatg
     8341 atttttgaca tattctttcg ccatgtgcaa agctgcggtg ccaccgtagc aagcctcttt
     8401 tacttcaaaa gaacgagcaa aagattggat tttcagtaaa tggtggataa ttaccgcact
     8461 cgcttttgaa tgatcaatcc cggattctgt tccgacgatg accatgtcga ttagctcacg
     8521 gtctttttct gttacgattt tgctggcagc atttgctcct agtgtaatga tgtcttcatt
     8581 tgcacgattg actgccattt ggtcttgacc aatccctata tggtattttg caggatcatc
     8641 cccacggctt tctgccaact ctgtcatatc taaatataaa ttaggaataa aaaaggaaag
     8701 acgatcaatc cctattttca tttcaacacc tctttactta tgataaaaaa cttactttac
     8761 taaggtatca taaaaagatt aaaagaaata taaaaaagag aatatttaag gattgttaaa
     8821 atttaatctt agaaaaaaat aactggaaca agagctattt gtttttacag caaagaatgg
     8881 tatatttttg taggaggtgg cttacttgaa agaagtcgtt atgatagatg ctgcaagaac
     8941 gccgatagga aagtatcgcg gaagtttaag tccatttact gcagttgaat taggcacact
     9001 tgtgactaaa ggattattag ataaaacaaa actgaaaaaa gataaaatcg atcaagtaat
     9061 atttggaaat gtcctgcaag ctggtaatgg tcaaaatgtt gccagacaga ttgctttgaa
     9121 cagcggtctc cctgtagatg tcccagcaat gacgatcaat gaagtttgcg gatctggtat
     9181 gaaagcagtg atcttagcac gccagctgat ccaattaggt gaagcagaac ttgtgatagc
     9241 cggcggaaca gagagtatgt ctcaagcacc tatgctgaaa cagtatcagt cagaaacaaa
     9301 tgaatatggt gaaccaattt ccagtatggt caacgacgga ttgactgacg cattttcaaa
     9361 tgcacatatg ggattaaccg cagagaatgt tgcaacacaa ttttctgtga gcagagaaga
     9421 gcaggatcgc tatgccttgt cgtcccagtt gaaagcagca catgctgtcg aagccggtgt
     9481 attttctgag gagatcatcc cagtcaagat ttctgatgaa gacgtgttat ctgaggatga
     9541 agcagttcgt ggaaatagta cattggaaaa actgggcacg ttacgtacag tattctcaga
     9601 agaaggaact gtaacagcag gaaatgcttc cccgttgaat gacggtgcct ctgtggtgat
     9661 ccttgcatcc aaagaatacg cagaaaataa taatctgcct tatttagcaa ccatcaaaga
     9721 agtagcggag gtcggtattg acccaagtat catgggaatc gctccaatca aagccattca
     9781 aaaattgaca gatagatcag gaatgaattt atcaacaatc gatctttttg agatcaatga
     9841 agcatttgca gcttcttcta tcgtagttag ccaagaactg cagttagatg aagaaaaggt
     9901 caatatatat ggaggagcaa tcgcattagg ccatccaatt ggtgcaagcg gtgcacgtat
     9961 cttgactaca ttagcttacg gattgcttcg tgaacagaag agatacggta ttgcttcatt
    10021 atgtattggt ggaggtttag gcttggcagt cttgttagaa gcgaatatgg agcaaacaca
    10081 taaggatgtg caaaaaaaaa agttttatca gcttacgcca tctgaacggc gcagtcaatt
    10141 aatagaaaaa aatgtattga cacaagaaac tgcgttgatt tttcaggaac aaacactttc
    10201 tgaagagctc tccgatcaca tgatcgaaaa ccaagtgagt gaagtggaaa tcccaatggg
    10261 gatcgcgcag aattttcaaa tcaatggcaa gaaaaaatgg attccgatgg caacagagga
    10321 accatctgtc attgcagctg caagtaatgg tgcaaaaatc tgtggaaata tctgcgcaga
    10381 aacacctcag cggctgatgc gagggcaaat cgttctatca ggaaaatcgg agtatcaagc
    10441 agtgatcaat gcagttaatc atcgaaaaga agagctgatt ctttgtgcaa atgaaagtta
    10501 tccttcaatc gtgaaacgcg ggggcggtgt tcaagatatc tctactcggg aattcatggg
    10561 gagtttccat gcatacttat ctattgattt tttagtggat gtaaaagatg caatgggcgc
    10621 taacatgatc aactcgattc ttgagagtgt ggcaaacaag ttacgggagt ggttccctga
    10681 agaagagatt ctattcagta ttttaagtaa ttttgctaca gaatcattgg cttctgcctg
    10741 ttgtgaaatc ccattcgaac gcttagggag aaacaaagaa atcggtgaac aaattgccaa
    10801 aaaaatccaa caggcagggg aatatgcgaa gcttgaccct taccgtgcag caacgcacaa
    10861 caaagggatc atgaatggta tagaagctgt agtggcagct acaggaaatg atacacgtgc
    10921 ggttagcgct tcgatccacg cctacgcggc aagaaatgga ttataccaag gattaacaga
    10981 ctggcagatc aaaggggata aactagttgg aaaactaacg gtgcctttgg ctgttgccac
    11041 agtaggcgga gcttccaata tcctgccaaa agcaaaagca tctttagcta tgttagatat
    11101 tgattcagcc aaggaactag cacaagtaat tgctgcagta gggttagctc aaaacctggc
    11161 tgctttgcgt gcattagtta cagaaggaat ccaaaaaggc cacatgggac ttcaagctcg
    11221 ttcattagcg atctcaattg gggctattgg tgaggaaatc gagcaagttg cgaaaaaatt
    11281 acgtgaagca gaaaagatga accagcagac agctatacaa atattggaaa aaatccggga
    11341 aaaataaaaa agcaatatct ttagaagaat aaagcaagat ttacagttat tttatttact
    11401 gcagatcttg cttttttctc tatcagaaaa tatgtttatt attagaattt tttatagtta
    11461 aggttaggca tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga agagaagtcg
    11521 cctaacattg taggatcata ggtgatttgt tggacaggtc aagctgatat ttgagcctga
    11581 tgttagaagg aatttctttt tacttgctgt agaaattatt atatgactaa aaaggctggg
    11641 attttaaatc ccagcctttt atgacaaata gaatttttat cgatcagtaa tcgctgtcgc
    11701 tgcaaattgt tcgatcaaag gaatcaaagt caatttgact ttgtatttcc gttcgagtgc
    11761 tttttcgata acgaactcat tttcagtagt catagaaata gttgctaatt tacgaggttc
    11821 atcagcattt ttttcattaa taactacttc atgttcattt tgttctgtgt aaaatttagg
    11881 atactcagca acggcttttg aaaaatcagc aaaacttaaa gcggcattcg tatcagttga
    11941 gcgtgtattt tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg gcacactgac
    12001 tgtttcttct gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt ttttcaatcg
    12061 ttgatcattc cagtaagaca cgatttcaat aagaagattg tcacggaatc gttcgaaaat
    12121 cccgttttct cctgaacgaa ttctctttaa tacagtattg aagttgtcat cgattggcac
    12181 gttgatccgt gtatcttcat ttgttttccc agctaatccg atcgttaaga tttgacggtt
    12241 tttgggatca agcatgactt gtaacggcaa accataacgt cgcaagctct cttcttctag
    12301 agaacggaat aattgtcctc ttacaccatc caatgaagga cggaattgat ttaataattt
    12361 ttgtttctgt tttttcttca tatgttctct ccttcgctca taccctttcg attataagaa
    12421 aaaaacagcc aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca aattacaaca
    12481 gaaaaatttg cgtattttcc cgttttttga aagaaaaata aaaaatggaa cggattatgt
    12541 atatatacat aatccgttcc attctttcta ttttttcggt atcaaggcaa aaacaggtag
    12601 ttcaggtgaa taaatgactt catgttcgtg ccagggtttg accattagcg aatgatcgaa
    12661 ccgttttttc agttcttcga tgatttgatc atgtgtccag tttcttgcag gaaaatgttg
    12721 gattttctct gcatcgatca aaaagacaaa atcggaatga aaagtctggg taacttcttc
    12781 cgagagagaa ttcaagcgtt cgattggatc cataaaatca cctttcgact taaaattggg
    12841 tagtatctgg gtcttttgct ttgccgacta caccatctaa ctgatcgatt tgtttcattt
    12901 cttcttctgt taattcaaaa tcaaataatt cagtattttc tttgattctg cttggtgtca
    12961 ctgacttagg aagaggaagg aattcgtgtt gcagcgacca acgcaaagca acttgtgcaa
    13021 tcgtcttgtc atgtgcttca gcgatttgtt tcatttcagg cacatcgaag attttaccag
    13081 tgcctaatgg actatatgct tcgaggatca tatcatgttt ttttgcatac tcaactacag
    13141 ttgtctgtaa ttcgcctggt gcaaggaaaa cttgattgac catcggcata atttttgctg
    13201 tttttgctag tgtatctaga tggtggggta agaaattgct cacaccgatt gctttgattt
    13261 tacctgcttc atacagttct tcaaatgctc gccatgtatc tgcatttgct tgttcccaat
    13321 tatcacgaaa agcaacaggg tttggccagt ggatcaaaaa cagatctaaa taatctgttt
    13381 ggagcttctt caatgattct tcaaaagaag acatcacaag ttcatagctg tggtttgcat
    13441 tccataattt tgttgtcaag aatatctctt ctctaggaat tccactttcc ttaatcgctt
    13501 ggccgacgct ttcttcattc ttgtatcctt gggcagtatc gatatggcga taacctgcag
    13561 ccagcgcttc tttaacagag gaaacagcga catccccatc aggtgtttgc catgtgccga
    13621 atcctactac cgggatttcg taaccatttg atagacgata agtgcttgtt aatgaattca
    13681 tactttcagc tcctttttta ttcgttcaac tataagtgta gcgcattttt cgaaaaaaag
    13741 aatccatttt gtttctactc ttgaaatttt tgtttaaaag aaaatgattt tgattctggt
    13801 atttttgatt ttttttccgt aaactaagac agaagggaat gagaaaatgg atatctattt
    13861 aaaaaaagca attctgcata ttattgacag agaaactggt tctccagtct tttcacaaaa
    13921 agagctggat ctgacaaaag aatatattcg tgattttctt caaaagaaga tccaaaaaat
    13981 ctcttctgct caaacaaaaa ctggacaact agcagaagaa agcatgatcg ttgaatcact
    14041 taaaatcgct caagaaaatt ttgtggaagc aagtgaagca atcgttcagc ggtggttcga
    14101 tatctaccaa gaaagtgaag aagcacctag tgcagatgta tttgttgtct tgtatgaatt
    14161 agatacagtg atgtacttat ctttattgaa agtcaattat cgtgaggctt acactcactt
    14221 tgttgaagca gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag caattctagg
    14281 ggcgaaaaca caaaaagcag atgaagcatt cgctgtgaat ttgactgatt ggacctacga
    14341 attaattgaa aaaaaatatg agttttctgg caaaaaagag ctttattttt ctggaagagt
    14401 gatcgaaagt gttcctgcac cttctttaga agaaaatgta aaagtgatcc aaaaagtagc
    14461 caagcatctt ggaaaaaaat ttgagacaga agaatttgat atcatggctg atttgaaaga
    14521 agctgtctat gatacgatag aagaaaaagg tcggttggat catgagatga ttgcggaaaa
    14581 agtcttcaag gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac aagagcaagg
    14641 atttgtccca gaagcacctc ctgtaaaaga agtacaagaa atttctgaga aaaaatttgg
    14701 aaaacaaaaa ttcaagctat caaacgggat cgaactgatc gtaccagtag atgtatatcg
    14761 caacccagat ttgattgaat ttgtgaataa tccggatggt acgatatccg tgatgatcaa
    14821 aaatgtggat gaagtattga atcgtttata aaaatgacta gaatattagg tgttcaagta
    14881 ccagtgatca gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga atataacaaa
    14941 aaaagagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    15001 ggcggaagca gaagcaactc cttcttattt cttggagtta aacgcttctg tcccagcctc
    15061 tttttatcta ataaaatttt aagaccattt actaattatc gattttttct gccgcatttt
    15121 taggcacttc agaagccttt atttcttccc agcttaatac acctttacga ggattgactt
    15181 tgagtttaag atacgcattc atctttaatg gatgttctct ttcttcgcga agcgtcacct
    15241 gttttttttc accatcttta ttataagccg cttgcttgta ggtataagtc gtgaatttct
    15301 ctccgttatc tgcttgggta gctgacattt cgccattcgt cgtgattttt gtataataag
    15361 cttcgccgcc gtaaaaataa tgataagctg caaaacttcc gccgagaaat atgcctaaaa
    15421 cgacaagtac gccaattaat tttttcatgt acttcacctc tttccttatt attaacgaaa
    15481 tagaagattt cgtctattga aagttgttgg caacagctga attttaaact tttcctcatt
    15541 ttgttcttat tgcttcaaaa gaaagttaag gactatcttg aagtttttct caaaaaacac
    15601 atttttttat ttataaaagg tacttttttc gcgtataaga cagacgagtg ctcttagttg
    15661 actttgacca atattgacgt ataataagct tagttcgaat cttacaaaaa gtaagtcgga
    15721 cgtattcttt ttgatttagt caaaaaaaga ctataataaa aaggtgagag ctgaaatttt
    15781 tcagtgatct atattattta ggaggtttgt aaattatggc aaaagaacat tatgacagaa
    15841 gtaaaccaca cgttaacatt ggtacgattg gacacgttga ccatgggaaa actactttaa
    15901 ctgctgcgat cactacagta ttaggcaaaa aaggcctagc aaatcctcaa gactatgcta
    15961 gtattgatgc agctccagaa gaacgtgaac gtgggattac gatcaatacc gcacacgtag
    16021 aatatgaaac agaaaaacgc cactacgcgc atattgatgc accaggacac gcggactatg
    16081 tgaaaaacat gattacaggg gcagcacaaa tggatggtgc aatcttagtt gtttctgcaa
    16141 ctgacggtcc gatgcctcaa acacgtgaac acattttatt gtcacgccaa gttggtgtaa
    16201 aatacctgat tgttttcttg aacaaagttg atttagtcga tgatgaagaa ttgatcgatt
    16261 tggtagaaat ggaagttcgc gagttattga ccgaatatgg tttcccaggc gatgacactc
    16321 ctgtgatcaa aggttccgca ttaaaagcat tgcaaggcga tccagatgct gaagctgcta
    16381 ttatggaatt gatggataca gtagatgaat atatcccaac accagaacgt gatacagata
    16441 aaccattact attgccagtg gaagacgtct tctcaattac aggtcgagga actgttgcct
    16501 caggtcgtat tgatcgtggt gctgttcgtg tcggtgatga ggtagagatc gtagggatca
    16561 aacctgaaac acaaaaagca gttgtaacag gtgtagaaat gttccgtaaa acgttagatt
    16621 acggggaagc tggggataac gtaggcgtgt tgttacgggg gatccaacgt gacgatatcg
    16681 aacgtggaca agtacttgct aaaccaggtt ccattactcc acatacaaaa ttcaaagcag
    16741 aagtgtacgt gttgacaaaa gaagaaggtg gacgtcatac tccattcttc aacaactatc
    16801 gtccacagtt ctacttccgc acaactgatg ttacaggaac aatcacattg ccagaagata
    16861 cagaaatggt catgcctggt gacaacgtga cgattgatgt tgacttgatc catccaatcg
    16921 ctgttgaaaa cggaacgaca ttctcaattc gtgaaggcgg acggacagta ggttcaggta
    16981 tcgttacaga gatcgaagca taagaaacgc gactcgaata ttttggtaat gaaaaatgtt
    17041 taaaataaaa gagcggtcca aacaggattt ctgtttggac cgctctttgt tcttcaattg
    17101 tataagaaga gattgtagaa gcatgaactt tcgttttact tcagtctctt ttattcatca
    17161 tgagagtgtt catctatcgg tatgcagttt ttaatacttt cttcgtatcc attttttcaa
    17221 accacttatt ctaccttgtg tctgctcaac tggcgcatta tcaataatca gcaaagcaat
    17281 cataacatat gcaatccaac tatctctaga atatagagta taacgtgaga cccagtttgt
    17341 agcgtacttg cttttatatt ctcttaatcc atggaaagaa taaaaatgag aaccaaactc
    17401 gtaaaccaag tatgcaatcc tttcctgtaa aaaactcttt ctggaagttc cgacatttga
    17461 aagaggggcc atccctaaat tgaaccattg gatatgttct tctttcatat aggcaaacaa
    17521 atgcaagaac aagaaatcca tactgccaga aggtgctttt ttagaatcat atcgcatcag
    17581 atcaatcgtt cctactttat tgttcgtgta agtaggcatg atcgttgcaa aagcgacaat
    17641 ttcttcttgg gtatttttga caactgcaat aggcgctctt tgtagataat cttcagagaa
    17701 aaagcctaaa gaaaaccctt tttcctttcg tgaacccaac caattatcag agatattttt
    17761 gaaagtactc atttgctcag tagaaaatgg tggttgtaag acatcaaatg taaatccttc
    17821 tcgttcgatc cgattcaaga cagcacgagt gcccttcatt tttttccctg aagttgtaaa
    17881 actattcagg tctacataag cttcttcccc catcttaatg aaatcgtagc cgaattcatg
    17941 caaaatcata acaatctctt cgcttgtttc gtagaaaact ggtaaatagc atagtcgatc
    18001 tgtttcttcg ataaaagctt cgattgcttc tggaaaatct tctttcttac cagaaggatc
    18061 acccattaca atacatttat tattgaagca agcgaattgg agaagaacag ttggttcacc
    18121 gtctttttca taggcaaaca tccgcttatc ttttaagaag atcaattgac tatcggagtt
    18181 tcctccataa gttgtaagga tcttcagggc cttttcttca ttaaaggctt cgccaatctg
    18241 tttcttttct ccttgaagga aatgaacaaa tagaacgatc atgaaactaa cagcgatgat
    18301 ggccaacaat cccgaaaacc aaatcttttc agaaggaaat aagaaaaagg aaataaaatg
    18361 gtgacgccga tgagggaaat ctggcagatt gtatacccca ataacgatgt aaagcaagga
    18421 cagtgcacca ataatgattc catcgatcgt acgccactcc catgaataga tcaattgttc
    18481 ccgaaataat tcgtttttag aagcaagaat gatcagaagc agaatagaca agaaaaccac
    18541 cggtgtgaaa ctaaaatcac ttaataaaac ataaagtaaa gctagaacaa tcaagaagat
    18601 tgtaggcaaa tatgctcgtt tcacacgtgc tgcgatccct ctgcccataa tgattaaaag
    18661 aaatcccaat agtatactag gaaattggat aataaaatga aatttcaatg ggttcaaact
    18721 atgaagccag cgaaactctg taaaagcttg cgggatagtg gcagaaagta ctagcatgat
    18781 tccagaaaag tataataaga caaccacgat tttatgcgca atttctgttg ctaattgttt
    18841 agggatacca gaataacgct gatcaaaagt ggaaccgata tttttgaaga aaaagacgat
    18901 tccaatcaaa aacggaatga tgtagtagaa caggcgatag agcaagatcc acacaacgac
    18961 tatttctctt ggcacaccaa tagcggacaa cccgataatc atcattacat cgaaactccc
    19021 aatttctcct ggaatcatag acacgattcc tatcactgaa gcagcaatga ataatggaat
    19081 tgtttgccac agatcaatcc ggatttccat taaataacca acagaaagga aactaaacag
    19141 gacaccggtc cattccaaca atgaaactag caatagctgt ccttgagttt taagtgaggt
    19201 accaccaagg aatcctttct ttttgaaagt agtaaataaa aagacgacag gaaaatatag
    19261 accgccgccg atcaaccata tccaatattg cttcaaaaaa ggagcaactg gcgtgaagac
    19321 tacaagtaaa aaagaaagca gactgtaaat agaaagtcca gccatcaaaa acaagaaaac
    19381 tttcgataat gcttggatca cgtttctgct ctctttcttt tgtccatata gttcagagcg
    19441 aagccccaca ctaacaaacc cgccgaaacc ggcaatattg ttgatcgtat tgatcagcca
    19501 gcttgtctca aacaggtaac gaggattctg cttttgttca agcatccgat tcaaaataat
    19561 atcataacca accataggta acacagagat aagaccaatc aatagcatta gtccgctttt
    19621 ccatacaggt atagtggcaa acgtttctcc taattgctgg atagatagag ttttaccgat
    19681 ggacatcaat tcaccgacaa taatgacaat gacagaaatc aaaaagattg ttttgaacag
    19741 tcctaggtgg tttttcagcc attgtatggt attttttaac aaataaatca ccttctattc
    19801 ctaatagtta tgtttcagaa agaaattgaa gaacttctcg attgaagcgt tcaggatctt
    19861 ttctagctaa ttcatgtcct tgttctttca caagcacaaa ggaagctttt ggtatagttt
    19921 tagctatata caaagaatgt tttagcttga ttacatcttt ttttcccacg atgatcaaag
    19981 tgggggagtt gatttttttt aaatcatttt ctgtaagccc gatgtcatgc agcaaaagtt
    20041 tgataactaa aagatttttg cgaagacttg ggcgaaataa agagagtaac catacccatg
    20101 cataatgaat attactgatg actctggcag aaaaacgaac gcctttaaca agcgtgtttc
    20161 cagaatttaa aatcaatcga tgaacttttt tagggaagct gctggcaaat accagtgcta
    20221 gatttgctcc atcactaaat ccaagaaaat ctgcctgatc gattttttcc agcaacatga
    20281 tcgtattcag atcctcggcc attaactgaa aattcaacat agatgcttcg tttgtcgaac
    20341 gaccatgtcc tcggctatct actagataca cggtatagta gcgttcgagt acagggacct
    20401 gttctgaaaa aaagcggccg ctgccgtcat tgccatgcag taaaaagaga gggaagcctt
    20461 gaccgctctt ttcatagtaa atttcgctcc catctggcat ttttgcatat ttcttttctt
    20521 ttttcacata atcacctact gtgtaaagta taacgattat atcagtcatt gggcaattgt
    20581 ttttactgaa aaacaacgat cattttcgta agatgtcaaa aaatacagga aaattcattt
    20641 tttcttaagg cgtacacaga aatcaaggaa gaaatagcca agaagataaa gaaatagtaa
    20701 gataaaaaaa gatgtcaaaa taactaggtg atgaaatgaa gcgaaaacga ttatctatta
    20761 tccgtattct tttttacatg atcattctga cggttattgg tgcggtgttg tttttaggag
    20821 gaagaactta tcgttcggta aaacgagtgg aaagctatca aatagaaata acaacagctg
    20881 caaaaaatta tcatatggaa aactacgaga atttgatcaa ggcgataata ttgaccgaat
    20941 caaaaggaaa agggacagat ctcatgcaga gctcggaaag tctgcatgga gaagctgaga
    21001 tcattgataa tccagctgac agtattgatc aaggtgtcaa atatttggcg gaaatgatag
    21061 tagaagcaaa gaaacaagga tgcgatttat ggacagctgt ccaagcatat aattttggga
    21121 aagattatat tgcctatgta aaaaatcatg gcggagaaaa cacagtagaa ttagcagaag
    21181 cgtattctaa agatgtgctg agcccacttt taggaaatga ggaagggaaa caataccgat
    21241 attggcggat ccagtccttg ctttacaatg gcggctatct ctatcataat ggaggtaaca
    21301 tgttttatcc tgatattgtc aaaatgaatg aaaaaataat agagacctat gaaaaagtct
    21361 ttaagtaaaa tagcaggaaa tgttcaatca tgaataaaat taaatgaaag aaggagtgcg
    21421 caaatagtgg aaaaaaggca aaaaatggaa aatcaagaag tggggtacac aaccaaagac
    21481 tggatgttac ggtttgtgaa gggaatgttt atcggttcag gatttatctt gccaggagtc
    21541 agcggtggtg cattagcagc tgtctttggc atgtatgagc ggctgatttc ttttttagcc
    21601 caccttacaa aaaatttcaa agaaaatgtt ttgttttttc tgcctgttgg tctaggcgga
    21661 gtgacaggca tctttctact ttctttttta gtcagttttt tattaggtgc gtatgaaaca
    21721 acgatattat ggttttttgt cggatgtatc gctggaactg ttcctgcgtt atggaaagaa
    21781 gcagggaaaa aaggaagaga tacttctgat gtgatcatca tggttgttac ttttgttctg
    21841 gcttatttgt ttttattata cggcgctcaa ttatttgacg ggcaagtcga tcagaatttt
    21901 ggaacatggc tgattgccgg cggattgatt ggtttgggta tgatcgtccc aggattgagc
    21961 ccttctaatt ttttggtgta tatgggattg tataaagcga tggctgacgg atttaaagaa
    22021 gtacgacttg aagtgattgt gccgatcgga attggtggga ttctttgtgt tcttggattg
    22081 tcaaaaatca tggattacat tttctcaaag gcatatagca agctattcca tttcatttta
    22141 ggtgtagttt ttgcttcaac gattatgatc attcctaccg attacgcggg attgggagtt
    22201 gctggggtac tagcttgtcc tgttcttttt atagccggta cagcactagg ttggtggatg
    22261 agccgtttgg aggaacaata taagtaaaga tagattgata ataaaaacag cacacgggtg
    22321 attcaaacaa aaaggttggc atcttttttg taaaatcatt cgtgtgtttt tctataaatt
    22381 ttataaaaac aagttgtttt taatcttgga acacgctaga cttaaacacg tgattatttt
    22441 tgaaagttgg cggaaaatgg agaaaaagaa aatgaatgga cttacacagc aagaggtagt
    22501 tcagaaaatg gcttctggcc agcaaaatga ctaccaggaa gatgcatcaa aaagtacaaa
    22561 acaaatattc agcgataata tattgacgtt attcaatttt ctgaactttg gtattgggat
    22621 ttgtctgtta tttgtaggtg cgtattccaa catggcgtat ttagcaatta ttgtcgtcaa
    22681 tatcgttatt ggaatttatc aagaaataca tgcaagaaat cttgtacgga aattatcgat
    22741 tgtttcccaa tcagaagtac tagtgatccg ggacggtaaa gaacaatcga tttctactaa
    22801 agagctggta ttaggagatt tggtaaaaat cggagcagga gaacagatac cagccgattt
    22861 acaggttata tctggacggg cagaagcaaa tgaagctctt ttgacaggcg aatctgattt
    22921 gattgtcaaa gaaactggag ctgaattgct ttctggaagc tttttgacaa gcggttctgt
    22981 catggctgaa gttatccgag taggtgcaga taattatgct gtccgcttga ctagcgaggc
    23041 aaagactcac aaagctattc gttctgaact tgtagattcg attcgcaaag tatccaaatt
    23101 cactagtttc gtgattattc ctttggggat catactattt ttggaagcac tgttcatccg
    23161 cgatgcttct atcaaaattt ctgttattgc atctgcagca gctttgttag gcatgcttcc
    23221 aaagggtttg gtattgctaa taagtattgc attaacgaca ggggtaacca aactggcaaa
    23281 aaaacgaatc cttgttcaag acatgtattc aattgaaacg ctggcccatg tcgatacatt
    23341 atgcttggat aaaacaggga ccattactga agggaaaatg agtatccagc gggtagaagc
    23401 actacatccg ctgtacgaga aagccatccc agaaattatg gggacgtatc tggcagaaag
    23461 cagtgataac aacatcacta tgcgggctat gagagattat tttgagcttt ctaatcggta
    23521 tgatgtgaca aacaaagtcg cattttcttc tgatcgcaaa tggggcgcga tggaatttcc
    23581 agaactggga gtcgtgtacg taggagcgcc tgaacgtttg gtggaagaag atcggcttcc
    23641 ggacagtcta aaaacagccc aagcgaatgg ctatcgcgta cttatgttgg ggatcgctcc
    23701 aaatcagcaa ttgaacgaag aaaaaccagc atttgttgag ccggttgctc tgtttgaaat
    23761 cgatgatcca atccgaaaaa acgctcaaga aacactagct tatcttcatg aggaaggtgt
    23821 ggatctgaaa gtgatttctg gagacaatcc ttttactgtc tccaatatcg ctagacgtgc
    23881 aggattaccg aattatgatg cgtatgtcga tctttctcaa attactgaag aaatggaagt
    23941 gcgagaagct gctcatcgct atacggtttt tggacgagtg agtccgcagc aaaagaaatt
    24001 gctggtaaat gaattgaaag aaagcggcag aacggttgca atgacaggcg acggtgtgaa
    24061 tgatgttttg gcgttaagag aagcagattg cagcattgct atggcagaag gtgatggagc
    24121 aacacgtcag attgcgaatc tggtattgtt ggactctgat tttactacat tgccagaagt
    24181 cctgtttgaa ggacgccgag tggtcaacaa tgtgacgaaa gtctcaggca tcttttttat
    24241 caaaacgatt tattcattta ttttatcgat tatatgtgcc gttacagcaa ttggatttcc
    24301 gtttatccct atccaaatca cgctgattga tttagcgatt gaagggtatc cttccttctt
    24361 tttatctttt gaacaagata aaagaaaaat cacttaccgt tatttgccta cagcacttat
    24421 taatgcttta ccaaatgcct tgcttgtagt agtgaatatc attgcggtgt atctacttgg
    24481 aaaaacattc gcatggggaa atgctgaaac tactactttg atgtattact tgctgattgg
    24541 gatcagttgc atggcagttg taaaagcttg ttatcccttc aacccattgc gcatcttcct
    24601 agcagtaacg acgatcgtag ggatatatgt agctgcaatg ctgttccatg gaattttgga
    24661 agtaaactta gggttgggag agagcgtagg atacttcttt gtattcatgc tcgtaaatat
    24721 tgttcttcga gtattgtact ggaaggctga tattcaaaat cagttactga aaagagtagc
    24781 aaattgatgc ttaatgagat agaaatgtgt tatacttgaa aaagcaatga aatatgatac
    24841 aatcaaacat gtataattgt gtcctttaaa atcgcactga tttcagttag tgcgattttt
    24901 taccggttat tctcctgaaa tatcttctgt taaacccaaa agatatcaat agagcagggg
    24961 gtgaaaaaat ggctcaacgg attcctcaag aggttatcga aacagtacgc agtcaaacga
    25021 atatcgttga ggtgattgga caatatgtcc agttgaaaaa gtctggtaaa aattacttgg
    25081 ggctgtgtcc ttttcatgaa gaacgtacac cgtctttttc tgttgcagaa gacaagcaga
    25141 tttttcattg ctttggttgt ggtaaaggcg gaaacgtgtt tacttttctt caagaactag
    25201 aaggtctaag ttttccagaa gcagtggtaa aagtggcaga cttcgaacag ataccgttag
    25261 aagatcaatg gcgtcagcgt actatagctg tccaaaatcc tgaatctgct acggggaaac
    25321 tgattgctgc tcatgaaaaa gcagcggaaa tctatcatca catgctattg catacaaaag
    25381 ctggacagcc agctttagaa tatttgcaaa gtcgtgggtt gtctttagaa ttaattgaag
    25441 aattcaacat aggttttgca cctaatgagc gttcttttct tcagcaagta ttcaagaatg
    25501 aatcaatttc aaatgaattg ttagaacgct ctggattgtt tgtcacgcat gatgatggcc
    25561 gtatgtctga tcgtttttat caaagagtga tgtttccact tcgtaattct caaggccaga
    25621 cgatcggttt ttcaggacgc tggcttgcac ctgaaaaagg gcaggaaaaa gatcagccca
    25681 aatacttgaa tagtccagaa acagaactgt tcaataaaag acaagtttta tttaatcttg
    25741 ataaagcaag aagcgatatc cggaaaaacg gagaaatttt gttgttcgaa gggtttatgg
    25801 atgtgattgc tgcatggcag gctggtatca aaactggatt agcttctatg gggacaagtc
    25861 taacagctca acagatccaa caaatggaaa agctgacgaa agaattggtc ttttgctatg
    25921 acggagacaa cgcaggtttt gaagcaacca atcgagggat tgaattgctt cggcaaaaca
    25981 gtcgattagc actttctgtc gtagtcgttc cagaaaagct tgatcctgat gaatatctcc
    26041 gtaagtatgg agaagcatct ttccaagaat tagtggctca tgggcgcgaa acagtcttta
    26101 cttttaaatc acgttattta aaacaaggga aaaatttaga taatgaaaaa gacaagatcg
    26161 aatacctcga agaattacta gttgaactta gccgagttgt ttcaccaatc gaacaagata
    26221 tgtatctatc tcaactgtca acagagtttc agatcggcag agagacgata caaaaacaac
    26281 tgcgcgatct tcgaaggaca aatcagcaaa atcgtcaaga acctcaggaa agtcaacaca
    26341 ctcaggttac gagacaagaa actgcacgga aaaagagacc tttgacgcaa gtagaaaagg
    26401 cagagcagat tctattatat cgtgcattta aagaagtttc tgtccgtaat attttaaaag
    26461 aacgagaatt tcagtttatt catgatgcat atgcagaagt ttatttttta ttcgatgcat
    26521 atgtctcaca acattatgaa ttcaaccttg cagagttttt agactttttg caagatgaaa
    26581 atttaaggcg gttggtggtg gaaatcgaat atttaccggt ggcagaagaa agttcgccac
    26641 gagaaatcca agacttgcta gatgtgatca gcaaatctgg cttagctgat gaaataacat
    26701 taaaaaaaca aatgcagcaa gaagccagac gaactggaaa caaacagctt gagcttgaat
    26761 tgacgattga aatcatcaat ttaacgaaac agttaaaaca agccaaatag ctactcaagg
    26821 ggggccttct tttatggcaa atggaacaga taaaaaaacg tatgaagcag cagtagcagc
    26881 tttcatcaaa gaaaacaaac ccaaacgaca agttgtatat gatgatttat ccaatcagct
    26941 tgctacacca ttcactttga atgcagatga aatggacaaa ttgatccaaa agattgaaga
    27001 cgcaggtatc agtgtggtgg atgaaaatgg tgatccatca attcacagct tgaagagtgc
    27061 tgagaaaaaa gctgaacaag caaaaacaga agatctttct gcgccaaccg gtgtaaaaat
    27121 caatgacccg gttcgtatgt atctgaaaga aattggccgt gtctcccttt taactgcgga
    27181 agaagaagta gcgttagcat tgaaaatcga agaaggcgac caagaagcga aacagcgtct
    27241 ggcagaagca aaccttcgtt tagtagtaag tatcgctaaa cgttatgtag gacgaggcat
    27301 gcagttcctt gacttgatcc aagaaggaaa catgggactg atgaaggcag ttgaaaaatt
    27361 tgactaccgc aaaggattta agttttcaac ctatgctaca tggtggattc gtcaagccat
    27421 cactcgagca atcgctgacc aagcacgtac gatccgtatt ccagttcata tggttgaaac
    27481 aattaataaa ttgatacgta tccaacgtca attgctgcaa gaccttggaa gagaacctac
    27541 gccagaagaa atcggtgcgg aaatggatct tccaacagaa aaagtccgag aaatcttgaa
    27601 aatcgcgcaa gaacctgttt cccttgaaac gccaatcggt gaagaagatg actctcactt
    27661 aggtgatttc atagaagacc aggaagcaac aagtccagca gaacacgcag cttatgagtt
    27721 actaaaagaa caattggaag atgtactaga tacattgact gatcgggaag agaatgtcct
    27781 tcgcttacgt tttggtttag atgatggccg gacgagaaca ttggaagaag tagggaaagt
    27841 gtttggtgtt actcgggaac gtattcgtca gatcgaggct aaagcattaa gaaaattgcg
    27901 ccatccttct cgttcaaaac aattaaaaga tttcttagaa taagcgtgga ccattgacca
    27961 gaagagaaat cagtaaaact gtttggttta ttttaaaaga aaaaaagctt gagactacgc
    28021 agtctcaagc ttttttttct ataggaagga aattcattca ttatttttaa gtaaatccta
    28081 aaaataagct attggcgtga cgtatagaat atgatagatt aaatggtagc gtaaacctag
    28141 taaaggagcc gatcgctaag tgatgaaaaa atgtccaaat tgcggatcga aaaaaatgga
    28201 gcagaatact atctgtccag actgcggtca tcaaataact gcagaagatc agcaaataaa
    28261 agaatcaatt gaagaaacaa atacagatgc agctatgcag gataaatcaa ctgaagatgc
    28321 agtctttcct gatacagaat taaacgatcc aatcgaatgg tcggaattga aagatttacc
    28381 tctagaatct gtaatggaat tattcgaaaa agcagaacca gaagacgaag aaccacctgc
    28441 tcacgatcaa gtaactaata tagagaaaaa tgaacagaag aaagcaactg ttcatgagac
    28501 ttcggaagaa tcaggaaaga atcaagaaga aacgcttgaa tcaaaacaaa aaagaagagt
    28561 agctgaactg aaagaaatcg tcgataatga ggaagaaaat tcgatactat ccgcttatat
    28621 caaagcacat cgtgaagata caaaagaaga gcacgcgaaa gagctgctca aaatgatcag
    28681 tgaaaaaatt gcacaggaaa acaaagtaga gccagaaaag gaacaagaaa ctgaaaaaga
    28741 ctctgataga gaaaacgcca caggtaaaga aacagtaaaa ataaaagacg taaaaacgga
    28801 tatcacagaa aaaactgaag tatttactga aaaacaatta gaaaaggaaa aaacgaataa
    28861 actgccggaa gcagagaaaa aagaaatact cgaaaacaca gacaaaaagt caacagagcc
    28921 cgatgaaata aatagtgaat ccgatgctga aaatcaatct gtaagcaaag agggtactga
    28981 tcttgaagca aaaatagaaa aagaagagaa ccagcctaag ctgtttgaag cagaagcgaa
    29041 atcaataaca atcgtgccag atactgaaaa aacacatcaa gaatcaaact tagaaaaaac
    29101 agatccagat aaagcgcaaa agaaaaaatc aaaaaaagta ccttatctcg ttttagcagc
    29161 agtacttcta ctcggtggag gaagctgggc ttattatgac catcatcaaa aagtagaagc
    29221 cgaagtagca gcagaagcaa aaagacagca acaaatggaa gaacttcatg agaaacttgc
    29281 tgccttttat acagatgatc accatcaatt tattcgtgca gatatgattc atcaagattt
    29341 atcaaaacta aaaaatgatt tatccagtct taagaatgaa aaaggatatt cagagttaga
    29401 aacaacttac caagatatcc aatctaagat ccgagatatc caaaaagtga atgagtggtt
    29461 catagcacct gccatcgtcg atgatcatct agcagatgac atcaagttga aagcagataa
    29521 agagatccaa gcaatcgaaa cggataacac agattttgga aaattgattc aacaagcaca
    29581 aacagaagct aaaaatcaat atgctgaatt gcaaacagct aaagaaaaaa cagctatagt
    29641 ctttaaagac ggaaaagtag tggattcagc aacacgcgac caatacaaga cggcaaaaga
    29701 agcagtagaa aaagtgaaaa atacagatct atctgctcag ttgaatgaac aattgaaaca
    29761 agtagataag atgctcactg ataaagagaa aaaagaagaa gaggaaaaga aaaaagcaga
    29821 agaagcgaaa aaagcagctg aagaacaagc aaaacaagcc gctcaagcag cagctgcagc
    29881 tcaagccgct caacaagctg cggtcgcaca agcagcacag caagaagcgg catcctcgac
    29941 cccttcctct tcaaactcag cgaatcagcc aatcaaatct gccagagcaa gtgatattgc
    30001 cgatgcatct aatccagcat ggacgtgggc accaggtgtc caaagcaaag tgattgctac
    30061 atgtatccaa cgtggatata tcgttgaagg aggttacaga ttagaaaaag ccagaatcga
    30121 aaatggggaa ggctattata atctatatgc cacttcaaca aaatctgcat tgatgaaagg
    30181 cattggagaa agtgctttgc cattttacat cgtaaccatc aactgtaaaa ctggatggtt
    30241 cggaggaaat gggagcaatt aattttaggc aaatgaaaga ggctgtgagc ctgccgttta
    30301 acaccgttta ttcgaaaaag gggagtatga cacaactgtt gtcatactcc ttttttcacc
    30361 tttccaaacc aaggctcagc tattcaagta gataacatta tgttatgata gcaaagaaat
    30421 gtattaagaa aacctttcac tatagaggga gaaaaaaatg aacgatttat tagcaactat
    30481 atttacaggt attattattg atgaaaatga aaaaaactac tttgtccaaa aaaacggaca
    30541 aacctttggc ttaagcaaat cagaaggtga acacaaaatc ggtgaagcag ttgaaggatt
    30601 cggttaccaa aatcaaaaac atgaaaatcg gatcactaca caaattccta aaagccgtat
    30661 cggtcattac ggttttggaa tcgttactgc tacacgaaaa gatttaggtg cgtttgtcga
    30721 tatcggtctt ccagataaag actttgtcgt atctcttgat gaattgccga caatgacaga
    30781 gctatggcct aaaaaagggg atcgcttgat gatttccctt agagttgatg caaaagaccg
    30841 tatttggggc agtttagcag atgaaaaaat attcaaatct ttaagcaaat atggaacaga
    30901 agaaatgaaa aacaaagatg tgataggaac agcataccgc ttgaaattaa caggtacgta
    30961 cgttttaacg gaagatttct atcttgggtt tatccatcct tctgaacgat ataatgaacc
    31021 tcgtctaggc gagcagataa aaggtcgagt gattggtgtc cgaccagacg gtgtactgaa
    31081 tctttcattg aagccaagag catatgaagc tatatcagat gatgcacaaa tgattttaac
    31141 cattttagaa cgttctgcag atcagcaaat caactttaca gataaatcgg atccagaaga
    31201 aatcatgaaa gcttttggta tcagcaaagg agcatttaag cgcgcattag gtaatctttt
    31261 gaaacaagga ttgattacac aagaaaacgg tgtgacaaaa ctagtcaaaa aatctaattg
    31321 agaatccctt gcatgttttt cttaactagc ttataataat tttaattaac atagtgttat
    31381 atcttttttt atttgtaatt attataaatt gttggggttg agtaaatggt catgaatgct
    31441 atttcggcga tgaaaaaaac aaaacagcag ctacatgaag cggggtttaa gctgacaccg
    31501 caaagagaag ctactctgct tgtgcttttg gaaaatgaga aagagcattt atctgcagaa
    31561 gaaatcttct ttttagtgaa aaaaaagagt ccggaaattg gacttgctac tgtttatcga
    31621 acccttgaaa tcttgacaga attgaaaatt gtcgataaag taagttttaa tgatggatta
    31681 gcccgttatg atttgagaaa agaaggcgcc aaacatttcc atcatcattt actatgcttg
    31741 aactgcggca cgatcgagga agtagaagag gatcttttac ttgaagtgga gcaagtagta
    31801 gaagaacgtt atcatttttt agtaaaagat catcggctaa cgttccatgg aatctgtcag
    31861 gaatgctatg aaaaagaaaa aagaaaccag tccgtctgag tgtctagacg gctttttctt
    31921 tgctcagatt atggaaaaag actatcagaa tgaagcggta tctgatatga tagagaggca
    31981 tgagggggat tattatgaaa gagcaaatca acgattatct ccactacttg aatattgaac
    32041 ggggattgtc cgccaataca cgacaaagct atgaacgtga tttggaacag tacttgcatt
    32101 ttctacagga gcagaaaatt gcttcatggc aggatgtcga tcgcttttta gtcatccgtt
    32161 ttctggagaa gttgcatgaa gaaaaaaaag cgacagcaac tgttactcgg atgatctcaa
    32221 gtctcagaag attccatcag tttttacggc aagagcgtat cactgatcat gatccgatgc
    32281 aacatatcga tacgccgaag aaagcacaga aattaccaag tacattgact ctgacagaag
    32341 tagaaaagct gattgaagca cctgatacga caaaaacgtt agggataaga gatcgtgcaa
    32401 tcttggaagt catgtatgct actgggatgc gcgtcagtga attgattgga ttgaaattgg
    32461 gagatcttca tctttctctt ggactggtac agacagttgg aaaaggagat aaagagcgga
    32521 tcattccttt aggtgattat gcgatccaat ggctggaacg ctacttagac gaagcacgtc
    32581 cattacttgt aaaggacgca tctgaaatgc atgtatttgt caataatcat gggaaaggtc
    32641 tttctcggca aggaatctgg aaaaatctga agcagttagt acgagaggct gggatttaca
    32701 aagaagtaac accacatacg ctgcggcata gctttgccac acatctgcta gaaaatggag
    32761 ctgatttgcg gacagtacag gaattgttag gtcacgcgga tatctctacg actcagattt
    32821 atacacatat cacgaagaaa agaatgacgg atgtttataa gcagcatttt ccaagggctt
    32881 agtcatagga aggtgagaag atgttaacac attaccggaa agaaaaccgt aaaattgcta
    32941 tgggactatt gagtttccat aagaaactga caggtaaaac ttctttgttg aaggagattg
    33001 atacgtacga atctgaagac gattatgaac tttttttctt tacgccagaa ggttcaacca
    33061 acattcaagg gattattggt atttactggg caacgaacga tcagctgatc atccatgaca
    33121 tttctttgaa tccgtcttat cgcggtgaga atatgggctt tcaaatgtta gatgaattac
    33181 aggaaaagta cccggatgtc tccatccgtg caactgaaat cacagagcct tacattggaa
    33241 aatggctagc aaagaaaaag aagaaagagt gaatgagttg acggagatca atattaaatt
    33301 agatgtattc gagggtcctc tcgatctgct gcttcatctg attcagcaaa tggaaatcga
    33361 tatatatgat ataccgatcg ctacgatcac ggagcaatat atgaattata tccatacaat
    33421 gaaaacactc gagctagctg ttgcaggaga atacttagtg atggctgcta cattgatggc
    33481 aatcaaaagc aagacattgc ttcctgtcca agaattcgtc acagacgatg aagaattatg
    33541 ggaagaagat cctcgagaag aattggtcac tcagctttta gaataccgta aatataaata
    33601 tgcagctgaa agactgacag aaaaagcaga ggaacgaagt ctttattaca caaaagaacc
    33661 tatgaacatc gatgggttag cggatgctga aaagccgttg aagcgaggac aagtcaaaaa
    33721 aatcgatttg atccgtgcca tgcagaaaat cttagaacgt aagaaatttt ctcaaaaagt
    33781 ggaaaaaacg atcacgcccg atgacacaac gatcgaagaa cgaatggtat ttatcgaaga
    33841 gcgtataaaa agaaatgcgc atggctgtac tttcgattct ttttttgagt caccttctaa
    33901 aagtgaagtt gtcactactt ttatggcttt gctagaattg atgaaaaaag ggcagatcat
    33961 tgcgcaacaa aaagaaaact atgaatcaat tatgctttat ccagcagtag gagataccga
    34021 acatgacgaa attaagtgaa cttgaagccc ttctttttat tgttggcgag gaagggatca
    34081 gtaatgaaga attaagcgga ctgttaaatc tcagcgaagc agaagtcaca agtttagtga
    34141 aagaattaaa agaatcttac actcaaaact cgcactcagc tttgcatatt tttgaaacgg
    34201 aaagccatta tgtattaacg actaaaaaag agctggctcc cttacttaag cgatatgcgc
    34261 aagggacatc aaacacatta tctcaagccg ccgtcgaaac cttagcaatc atcgcttata
    34321 aacagccaat cacaagagct gaaatcgagc agattcgagg agttcaggtt tcaggagcgg
    34381 tacagcgtct agctgcccat aaactgattg aagaaaaagg tagagtagaa ggaccaggtc
    34441 ggccgatatt atatggaaca actggctact tcttagatta ttttggtttg aaatcattag
    34501 aagaactgcc tgatacacaa gaaatggaaa tttcacttga agaagacgtg tcaccagatc
    34561 tattctttga tggaatgaat aaaggagaat aattgtaatg gagagattgc agaaagtaat
    34621 tgcccacgct ggcatcactt ctcgtcgaaa agcagaagaa ttcatccttg caggacgtgt
    34681 aaaagtaaac ggacaagttg taagggaatt agggataaaa gttgggaaac atgacactgt
    34741 ggaagtggat gaagtcccaa tttatcaaga agaatacggt tactatttat tgtacaaacc
    34801 taaaggagtt atttctgctg ttgcagatga caaaggaaga aaagttgtta cggatctttt
    34861 gccgatggta aaagaacgta tctatcctgt aggtcgactg gattacgata cttcggggat
    34921 cttgctactg acaaatgatg gagattttgc tcagcgcctg acacatccaa aacatgaagt
    34981 ggataaagtt tacgttgcaa aggtaaaagg gatcgctaca aaatcgatgc ttgcaccttt
    35041 gacaaaagga ataaagatcg aaggacgaaa aacatctcca gctaaattcc aaattttatc
    35101 agtggatcct aagacaaacc acagtatcgt ggaattgacg atccacgaag gacgtaatca
    35161 tcaagtgaaa aatatgcttc aagcagttgg tcttcctgtg caaaaactaa aacgtgaaaa
    35221 atacggagat ctgactttac aaggcctacg tcctggggat taccgtgaac tgaacaaaaa
    35281 agaagtcagc agtttgctga accactcaaa atgaacactt actttttata agtgttagat
    35341 gttgcgtttt ttgaatagat atgtataatg aatatgtaaa caaaaattat ataaggttcg
    35401 tcttcagggg cagggtgaaa ttcccgaccg gtggttatag tccacgacct attttgcaaa
    35461 aaaatagctg aatcggtgaa attccgatac cgacagtata gtctggataa agaagataga
    35521 gcttatttga cgacgcttga tcaaataact ctaactctat tttccctgga aaatagagtt
    35581 ttttcatttg gagagaaacg tcaaaaagtt cgctgaagag gagtcctaac aggaggattt
    35641 caaatgaaga acacccgtgt acagaaaatg gttgcagtag cgatgtttgc tgcgatcgca
    35701 ttagttttac agtattttgc ttttccgatc atgccggctt ttagtttttt gaagatcgat
    35761 tttagtgata tctcggtatt gatcagcatg ttcttattcg gtccagcggc cggagttgca
    35821 acagcgttct tgcgttcagt attacatctg ctgactacag gtttttctcc ggataatttg
    35881 gtaggagaca ctgctagttt cttatcaaca acgatcttta cattcccgat ttattatttc
    35941 tttagtgtaa agaaacagca taaaggaaga aacaaatttt taggtgtcat tacaggaacc
    36001 atcgctctaa ctatttttat gagtatcgca aattatttcg ttattacgcc tctttactta
    36061 atgtttttag ggctgaacgc aacacagatg ctgggcatgt ctttagcaaa ctatgtactg
    36121 attgggatcg ttcctttcaa cttgatcaaa ggattcatcg tgagtgctgt ctttttagtg
    36181 ctgcatgcgc aacttcttcc atggttaagt cgaaaacaac atcagttcga acaacgtcat
    36241 acagcagtca aataaatatc aaaaagatca agaaatttct tggtcttttt ttttggaaaa
    36301 aaatacaaga catgcaataa tgtaaacgtt ttatttgttt gttttgagta tttttttgat
    36361 ttatgcataa aaatcaatca attctttgca aatgtgaaat tcttctcctt ttaaaagacg
    36421 aaaatgtttg tttataaaaa aaaggaaacg ctacaatgaa aaagaagaaa gctttaaaaa
    36481 cctgaaaaaa gaggagggaa tagggaaaaa cgaacttttt gaacaaaagt tttacaaaat
    36541 gcgtgttatt atttttgttc tgatactctt tttgataaaa taacattaga gccaaaattg
    36601 ttgtctctaa cggaaaagaa aggaagaaga agatggaaca aacaaaaaag aaaaaatttc
    36661 aaatgccttc agcgtatacg gtcttattta ttattattgc agtaatcgct gtacttactt
    36721 ggtttatacc agctgggcac tatagtgtag acgatgcagg caacatcgtc gctggatcat
    36781 atgaacgagt acaacaaaat cctcaagggc tgtgggatat tttcatggcg ccaatcaatg
    36841 ggatgctagg tgtaccagcg ggggagctaa ctaaagaaac acctgctgca atcccaatct
    36901 ctttcttcat tttggttgtt ggtggatttt taggtattat caacaaaaca ggggcactag
    36961 atgccgggat tgcttcagtt gtgaagaaat tcaaaggaaa agaaaaaatg cttattccag
    37021 tattgatgct gttatttgca ttaggcggct ctacttacgg aatggcagaa gaaacgattc
    37081 cattctatgc tttactgatc ccagtcatga tggctgtagg ctttgataca gtagtagctg
    37141 tagcaatcgt attagtaggg tctcaagtag gctgtctagc ttcaacggta aatccttttg
    37201 caacaggcgt agcttcacaa gctgttggga tttcgatggg tgaaggtatt ggtcttcgat
    37261 tcttgatgtt cgttattttg ttagctattt ctattgtata tgtgtatcgt tatgcatcaa
    37321 agatcgaaaa agatccaaca aaatcattag tatacaacca acgtaaagaa gatatgaagc
    37381 atttcgatat tgaagcaatc ggtcgtcaag aagtaatcac gaaaaaacaa aaacatgtta
    37441 atgtattgtt ctttattact tttggtatca tgatcatcag tttgattcct tggacaacct
    37501 tgaatgcgaa tttcacgatt tttgaatcat tgacaaactg gttaacaaac cttccggtac
    37561 taggtacggt tctaggtaaa agcatgcttc cattaggtga ctggtacttc ccagaaatta
    37621 ctatgttatt cttagtaatg tccatcgtag tagcaatcac ttatggcatg aaagagtctg
    37681 aatttgtctc tgaattttta gctggtgctt ctgatttgat cggtgtagca atcgtagtag
    37741 ctgttgctcg tggtatccaa gtggttatga acaatggatt aatcacagac accatcttac
    37801 attggggcga acaaggatta agttcattat cttcaagtgt attcatcatt attacattta
    37861 ttttctatat ccctatgtca ttcttgattc cttcaacttc cggcttagct gctgcaacaa
    37921 tgggaatcat gggacctatg ggtgaatttg ccggcgtcgg taaggattta gtaatcactg
    37981 cttatcaatc tgcatctggt ttagtaaact taattacacc aacatctgct atcgtaatgg
    38041 gtgctgtagc aattgctcgt ttcgatgttt cagtgtggtg gaaatttacc ggtaaattaa
    38101 ttgcgatatt atttgtagcc atctgtgtga tcctaggtgt agcagcaatg ttctaaacct
    38161 agacaaatac aggtacaagg tctatcataa gaatgaagaa accgataaac ggctatcgat
    38221 aagcacgtgg ataagtcaat agctccattc agtgagtgtt ttttctttca tcatttgtta
    38281 agaacattct gcttgctgat ggagcaggct taaaacgtca ttatttgaga agaagaggtt
    38341 gtgtcggagg aatttgacat gacccttttt aacatattag gaggataata tcatgaaaaa
    38401 atttgtgaca gaacaacatc atgaacaagc attagaatca ttgagcgaat tgatccgaat
    38461 tccttccgta ctagatgaag cagattcagg acaaggacat cccttcggta aaaaagtgat
    38521 tgaagctttg gataaagtat tggaaatcag tgaaaagcta ggatttagaa catttaaaga
    38581 tccagaaggc tattacggtt attcagagat tggctctgga gatgaattat tcggtatctt
    38641 gtgtcatatg gacgttgttc cagcagggga cgaaaataat tgggaaacaa aaccattcga
    38701 tccaactata aaagacggct ggttagttgg tcgcggatcc caagatgaca aaggtccttc
    38761 tattgcggca atgtatgccg tcaaagcttt gattgatgct ggcgtggaat tcaagacacg
    38821 tattcgtttt atctttggta cagatgaaga aaatttatgg cgttgtttag aaaaatataa
    38881 cgaaaaagaa gaaggaatta cgcaaggatt tgcgccagat gctgaatttc ctttaattta
    38941 tgcagaaaaa ggcttattgc aagcttactt gacaggacca ggaacgaacg agttttctgt
    39001 aaaagctgga ggagcgttga atgttgttcc tgatgcagct ccttactctg gagaaaagct
    39061 ttctgaagta aaagaagcat tgaaaaaaca cgaattcgat ttcgaagaac aaggtgaagg
    39121 catcgtcgtt cttggtaaaa gtatccacgc gaaagatgca gcacaaggag tgaatgctat
    39181 ttctcgtctg gcaattgctc tttcagaagt atttgatttt ggcccaatca atttcttagg
    39241 aaaattagtt caagaaaatg caactggtga agctgtcgtg ggcaaaacag aagatgaaca
    39301 atccggcgag ctaacgatga actttgcaag tttggaaatc acaccagaac aaacaaaaat
    39361 cggtgtagat atgagaatcc ctgttacatt caagaaagat gatttagtag ctaaattaac
    39421 taaaacagca gaaaaatatg ggttgactta tgaagaattt gacttccttg attcattata
    39481 cgtgccacta gacagtgagt tagtgaaaaa tctattgggg acatatcgcg atattactgg
    39541 agacatgact gaaccgtttg tatcaggcgg cgctactttt gctcgtacga tgaaccagtg
    39601 tgtggcattc ggtgcgatgt tccctgatac tcctgacttt atgcaccagg cgaatgaacg
    39661 atgggaatta agtagtatgt ataaagcaat ggaaatttat gcagaagctg tgtatcgttt
    39721 atgtgctaaa taaaaataaa catacaaaaa gggcggatat tcaaatggat attccgtctt
    39781 tttttatttg atgctggaaa gtttgcattt ctctcttatt tttttaaggt ataatcatac
    39841 ttgattaaga aaaaggggtc actcagatag aaaaggtggg agaaagtgaa aatatttaga
    39901 cgaatactaa ttggcttagt catcatttta ggattactcg gtggagccgg ttattactat
    39961 atacagcaaa atacgtacga accatcgtca gaagcgcaag tgttggtaaa agaaggaaaa
    40021 aacaaagaag attacttatt ttttgaagga gagaaaaaga atcatggaaa gtctccatta
    40081 cttatttttt atcctggtgc tctcgtagag ccagaaagtt atagtatttg ggcgcaagat
    40141 ttagcaaaaa acggcttttc agtcgctatt gtcaaaatgc cacttgatct agcagtgatg
    40201 ggcggaaaca aagcggataa ggtcaaagaa gattttccaa attccgatta tgtgatcggt
    40261 ggccattcat taggtggcgt aatggcaagt cgttatgtca ataagaatca ttcagacaaa
    40321 catttaaaag gggtattctt catggcaagt tatcccgaca aaaaaggaac attgacagaa
    40381 tcagctgttc ctgttttatc gatcacggcg acaaaagata aagtattgaa tcaggaagct
    40441 tatcaagaag ctaagcagta tttaccggaa agaacagagt atgtatcaat caatggcgga
    40501 aatcatggag gttttggcag ctatggtgag caaaaaggag atggagaggc agaaatttct
    40561 aacgcagacc agcaaactgt gatcgtcaat acattggaaa actggctgaa aaagttgaaa
    40621 taatataaat gggtcctata gggtccgtga aaaaactagt caattaacta tagatagcaa
    40681 atgaatcttt caaaataagt cgggatttca cggaacaaga gaaacagatc ccggcttatt
    40741 ttcaagttta gagaacttag tgtacgtttt taaagtttcg tttatattta tattttattt
    40801 tctaataaaa tcgctcgaac tggacatttt tgatacgctt tttttacata tgtttcttct
    40861 tccggcatca cgaactgttg atgagcctct ggttcttggg caaataaaac gattccttct
    40921 tcatcataat cgaaaatatc tggcgcttcg atttgacaaa gaccgcatgc aatacagcgt
    40981 tcaggtacaa gtttacatag tagtgacatt tctaaccatc ctaacttaag gagaataata
    41041 tgattgtttt tattttatcg ctattttcat caggacacaa gctgcggatt agttctcttt
    41101 accaactcct tgtgggaaaa cgaacgactt cagtacttat ttatggattt acccacgaac
    41161 ttttgtttat ccataacagt tttccagaat tgaagcagga taagttttat caaattttgc
    41221 aaaaaattgc gcagcaaggt tggatcgaaa taaatgaaaa tgaagcaaag ctaacgcctg
    41281 caggggctga tatgttgtcc gaacatcaaa tagaacatac tggtttgcgt tttgaccgct
    41341 atggcagaac aggggaaact agttggcggc tcattaaatt tgctgttcaa gtaatttcta
    41401 atctggcaac gggtattcaa gactatcttc cagcagagac aagtcctttt tatactttcc
    41461 aactaaaaaa atggcttagt gaaagccaat tgccacgtga aattttgatt gatactgcgt
    41521 atgagagttt ggtgcagctg ttttcggaga taccagaaga agcagcagat tttttagcta
    41581 accaattatc tggaaatgag cgaacagggc tgcttcctta tcagctggca aaaaacaatg
    41641 atgagtcagc ggtttatctg caacaaagca gatgtatcca ccttttgtta gcacaaatcg
    41701 aaaaacggcc agattcgctt tggcacgctt tgatcgattc attattacag caaaatttca
    41761 atcaaagcat gatgatcacc aagcagatgt ttatgaatgg tcagacaatt gaccagatca
    41821 tggcaatccg tcatttaaaa agaggaacag taacagatca tcttattgag tgggccttgt
    41881 tttttgatga ttttccatac gagtggattc tctctgcaga aacaatcgaa cgtttggaac
    41941 caaataagga ttccgtgcga gagtggcgct ttagcgaatg gaatgtagat ggacaattag
    42001 attatggaga atttcgtttg tatcaaatct atctattgag aaaggaagcg atacagaatg
    42061 ttaataaatg aattaaaaaa atattttgga tttgattcat ttcgtcccgg ccaagaagaa
    42121 attattcagg cgttgcttga tggagaagat actctcgcta ttttgcctac aggaacggga
    42181 aaaagccttt gctatcaatt gacggggtat ttaatggaag gtctggtcat catcgtgtca
    42241 ccgctgcttt ctctaatgga agatcaagtg acgcaattac aaaaacgagg ggagaaacga
    42301 gtagctgctt tcaatagttt actttcgcaa tctgaaagaa gatacgtcct ccgtcatcta
    42361 tcgcaatata aatttttgtt tttgagtccc gaaatgttga ctaatccttc tcttttagag
    42421 catctgaaaa aacaaaatat tgcgttatat gtagtagatg aggctcattg cgtgtctcaa
    42481 tggggagttg atttccgtcc agaatatcaa cagttaggaa aaatccgaaa gcttttagga
    42541 aacccggtca cactggcact aacggcgaca gcaacagatt tagtagcaag agatatacgt
    42601 catgtactat ttgatcgacc accaaaagag atcagacaat cagtcaatcg gcaaaatatc
    42661 tcgctttttg ttcggaaaac gcagcaaaaa gaacaagaac ttgaacactt catggaaaga
    42721 gcacaaggag cggcaatcat ttattgtgca acaaaaaaag aagtcgaacg attgtatcat
    42781 ttattcaggg aacgttttac agttggatac tatcacggtg gtcttgatgc agctcaaaga
    42841 agacaacttc aacagcagtt tgtaaaaaat cagctgcagt ttctcattgc tacgaatgca
    42901 tttggtatgg ggatcgataa atctgatatc cgttatgtgg tccattatga tttgccagac
    42961 agtctagaaa actatgtaca agaaatcggt cgagcaggca gagatcagga agaaagtgca
    43021 gcaattcttt tttatcaaag tggggatgaa cgtatccatt atttctttaa tcaactttct
    43081 agagaacagc gacaaagttt tgagctttat ttggaatatg ccacagagca agctcccttt
    43141 gatgagctgc aaaaaaaatg gatggagctg atccagcagt ctgaaaaacc agaaaattgg
    43201 gtggaacggc tcaaacgtca ggaaaaagaa aaagaattcc gcctgcagca gatgcttcgc
    43261 tatattaatg aagaaaactg ccgcagaaaa ttcatcttag cttattttgg cgaaaaactc
    43321 tccgaaaagc cgcagaattg ttgtgatatt gatggagcaa aagcagaact tctgccaaag
    43381 aggaagcttt tttctaagac tgaacaactt cattgggaaa cgattttact aaatttattt
    43441 aaaaaaaaca tggaacattg attttttagg acaagacact ttttcctatg gtataataca
    43501 cacgatagag tttaggaggt aaaacagtga gcagaaaaga tagacatcaa tctacagaaa
    43561 ataacgaaac acaagaacca tgggaacaac caatctacga tacagatgat gaagtttcat
    43621 caagaagttc tcagagacaa caaaagaaag gaaatacatt atttctttcg ctatttttga
    43681 ttttactggt cttatgtatc gctattccag caggtgctta tttctggatc agaaatggat
    43741 caagcaacaa tgcaacagca gcaacttctt ctgaaacttc gacatcagta gtagaaagtt
    43801 ctacaagtac aacaaaagag gagtcaactg agacttcaac agtagaatct agtacaatcg
    43861 cggaagaatc tactgtgtct caagatacag gtgtaacgtc agaaagcagt attgcagcag
    43921 agacaacacc gacttcctca agtgaggcaa cttatcaagc aggggattca agcagcaatg
    43981 cagcagaaac aacaccttct tcatcagctg cagccggttc aacaacgact gttcaagcag
    44041 gcgaaggtcc aaaacaagtg gctgagcgtg cagggatcac cactgatcaa ttgtttgaat
    44101 tgaatggctt agatccaaac aactttatgc tttaccctgg tcaagaatta agaattaaat
    44161 aataatagcc gcgctttgcg gctttttttc tgagaaaaag gagttatatc atgacacaaa
    44221 tcagtattgc aatcgacggg cctgcttcat ctgggaaaag taccgttgcc aagatattag
    44281 caaaagaatt gaattatatt tatacagata ccggtgccat gtatcgcgca gtaacttatt
    44341 tagctattac gaacaatgta tctttttcag atgaagaagg gctagtttcg ctgatcaaaa
    44401 agtatcctat ctctttttcg caacaagaag atggacagca tgtattcata gcatcaaaag
    44461 atgtgacttt agaaatccgt caaccagatg tcacaaaagc tgtttctgaa gtctctgcac
    44521 atggaaaagt cagagaagaa ttagttgctg tccaacgaaa aataggggaa cgcggtggcg
    44581 ttgtcatgga cggaagagac atcgggacag ctgtattacc gaatgcagaa gtgaaaatct
    44641 ttttagtagc aagcgtaagt gagcgtgcag aaagacgttt caaagagaac caagaaaaag
    44701 gaattccgac tgatcttgaa acactgacga aagagataca ggaaagagac cattatgatt
    44761 caacaaggga cgtctctccg ctgaagcaag cagaggatgc tgttcgtatc gatacgactg
    44821 gcaaatcgat tccagaagtc gttgcagcaa taaaagctgt tgttttgaaa aaaggttatc
    44881 ttctttttta aattaagaaa aaaaatgaaa aacaattgtt ttttcaagga aaacgaacga
    44941 aatcgcttta ttttttttga ataatcaatt aaactaaaaa cagtaccgta cttacggcag
    45001 aatgttggtt aggaggacaa gtgttatgac agaatttgat caaaatcaag agaacaacca
    45061 gtcaatggaa gacgcaatga aaagcgtaca agaagtaaaa gttggcgatg ttgttaaggg
    45121 cgaagtttta gtcattgaag acaaacaagt gattgtcgga atcgaagggg ctggagttga
    45181 aggagtcgtt ccggcaaaag aattatcaac tttgccggta gaagatatca gcgaagtagt
    45241 aaaagtcggt gatgtattag atttagtcgt tatcagtacg atcggcaaag ataaagaaaa
    45301 cggcagctat ctactttcaa aacgccgttt agatgctaaa aaagtttggg aagatatcga
    45361 aagagatttc caagagggca aggtcatcga agcacctgtt acaaatgttg tcaaaggcgg
    45421 tttagtcgtt gacgtcggtg tacgtggttt tgtccctgca tcaatggtgg aagatcattt
    45481 tgttgcagac ttttcagact ataaaggcaa aacattgagc tttaaaatca tcgaaatcga
    45541 accatcagaa aatcgtttga tcttatcgca taaagcggta gtggaaaacg aaagagaaga
    45601 aaagaaaaaa gaaattcttt caactatcca tgagggagat gttctcagtg gacgtgttgc
    45661 acgtcttact gattttggcg catttgtcga cctaggcgga attgacggtc tagtccatgt
    45721 ctcagagatt tcacatagtc atgtagcaaa accaagtgat gtattggctg tcaatgatga
    45781 tgtaaatgtc aaagtgttgt ctatcaatcc tgaacaagaa cgtgtttcct tgtcaatcaa
    45841 agacacacag cctggacctt ggtcagaaat cgatacaaaa gctccagttg gcacagtatt
    45901 agacggaaca gttaaacgtt tgacaagctt cggtgccttt gttgaagtgt tccctggagt
    45961 agaagggcta gtccatatct ctcaaatttc acataaacat attgcgacac cacatgaagt
    46021 gcttcatgaa ggtgacgaag tgaaagtgaa agtattagag atccatccag aagaacatcg
    46081 tattgcattg agtatcaaag ctttagaaga aaaaccagct tcagaagaag aaccaaaaaa
    46141 tgttgagtct tatgaattac cagaagaaaa tactggattt actatgggag acattttagg
    46201 tgatgcgttg aaagaagaag tagaagaaaa agcaaccgat gattctgaaa aataattgat
    46261 tttgtatctg tgactgagat gaaagcagct gcttttgtct cagtttttct tttgaaaagc
    46321 gattagtatt tcaataagga ataaaaatac ttcgtttaat tgacaatcta tgcttgaaag
    46381 ttggcaatgc attcgttaaa ctaagtgaga ttggaaactt gtgaggagga aataatatgg
    46441 caaatccaac aattgcaatc gttggccgcc cgaacgtcgg taagtctacg atttttaacc
    46501 gcatcgctgg tgagcgtatc tcgattgtcg aagatactcc tggagtaact cgtgatcgta
    46561 tttatgcaaa aggtgaatgg ttaggtcgag aattcagcgt gatcgacaca ggcggaattg
    46621 atcttggcga tgaacctttt atggatcaga tcaagcatca agcagaaatc gcgattgaag
    46681 aagcagatgt aatcatttgt gtagtcagcg gacgcgaagg cgtgacagac gcagatgaga
    46741 tggttgcgaa aattttatac agaagtaata aaccagtgat tttagcagtc aataaagtag
    46801 ataatccaga aatgagaaac gatatctatg aattttattc cttagggcta ggcgatcctt
    46861 atcctgtttc aggaagccat ggactaggga ttggtgatat tttagatgaa gcagtgaagt
    46921 atttctctga agagactgaa gaagaggacg acgacacgat taagtttagt ttgattggtc
    46981 gcccaaatgt tggtaaatcc tctttgatca atgcgatttt aggagaagaa cgtgtaattg
    47041 tttcggatat tgaaggaacg acccgagacg caatcgacac ccacttcgtt tctgaaagtg
    47101 gtcagaaatt cttaatgata gatacagctg gtatgcgtaa acgcggaaaa gtatacgaaa
    47161 atactgaaaa atacagcgtg atgcgtgcaa tgcgtgccat tgaccgttct gatatcgttt
    47221 tgatggtact gaatgctgaa gaagggattc gggaacaaga caagcgagta gcgggttacg
    47281 cccatgaagc tggtcgtgga attattatcg tcgtgaataa atgggatctt gtaaagaaag
    47341 aaaccaatac aatgcgtgat ttcgaacaag agattcgtga tgaattccgg tatcttgatt
    47401 acgctccaat cgtttttgtt tctgcagtga ctaaacaacg gttggaacgt ctgcctgaaa
    47461 tgatcgaaca agtaagtatg aaccagaatc ttcgtatctc ttcggctgtg ttgaatgata
    47521 tcattatgga tgctgtagca atcaatccaa caccaacaga taaaggaaaa cgcctgaaga
    47581 ttttttatgc tacacaagtc gctgtcaaac cacctacatt tgtcgttttt gtcaatgaag
    47641 aggaattgat gcatttttct tatgaacgtt tcttagaaaa tcaaatcaga aaagcattta
    47701 cttttgaagg gacgtccatc cgaattatcc ctcgtaggag aaaataggca ttttaccata
    47761 tcgacgtttt tttttcaaaa gaaaaagaca gtttcgtttg ttacgtttct atgactaaaa
    47821 aaagcatgtg aaaattggta aaaaccttgc tattaagcgg ttcatatgat atcgtgataa
    47881 cagaatattg aagttaatca atatttcttt ttaaacttct ggaccactca gaaagtttta
    47941 actaaatgat atttctatca tatctcttgt ggaggaggtg aatctatcca tggcaaacaa
    48001 agcagaatta atcgaaaaag ttgcttcagc tactgattta actaaaaaag atgcaactgc
    48061 agcagtagat gctgtatttt caactattca agatgcttta gctaacggcg aaaaagttca
    48121 attaatcggt tttggtaact ttgaagttcg cgaacgcgca gctcgcaaag gtcgtaaccc
    48181 acaaactggt gaagaaatcg aaattccagc tagcaaagta cctgcattca aaccaggtaa
    48241 agcattaaaa gacgctgtta aataagacag ttgaaaaagc gcttgtggct ctagggctac
    48301 aagtgctttt ctttttattt cctagcagaa aaggaataaa taagagacag agattccgaa
    48361 tccgtatgtc atacatagaa aacacatgta ttttatttaa tttttattta tttgcaagtt
    48421 atgaggtgag ctttgctctt atcttttcta gaatttgctt actaatggta aaataaaaat
    48481 aagaagagga gggatgcgta tggaacttga ttatcaagta gaagatgcgt tgacgaaagt
    48541 gttttacgga gaaacgctag tcagtcagct aagagaactt tccattgatc agccaatctt
    48601 atttttaacg aatcagcgct attatgattt gtttgcagat aagatcaacc cgttatttgc
    48661 aaatcaagca aacaatgact ggtatatttg tgccaacact caatgcaatc atttaacaga
    48721 attaaagaac ctgcttgatt tcgtcaaacg ctatcccgaa aatcaatcca tgtttatcat
    48781 cggatttgga aatgaaggga tcatggatct ggcaggtttt tttcaaaaac atacaaccct
    48841 tgattcatca ctatggatga tccctgtatc gatccgttct ctaagccgag cattaacacc
    48901 gcagcggacg attttgcagc taccaaatcg tgtagtgctg caaaccatca atctgccaga
    48961 acgcatcgtt tatgatcaaa caatcagcga gcggcaggtg gacggaaagc agattgactt
    49021 tttgactttt atctgttgcg gaatgatttg tgatcatcgt tttttgcaaa atctgtataa
    49081 aaactatcca aataaaaagc aattaacgac tcggccgttt gccgggatga tcgaagaaat
    49141 gatctatttt tatcaattag aggcagaaaa cctgtcaaat tatggtaaac tgtttgaaca
    49201 agctttttat ctgaccgaaa atggccatct actttctgct agtatgaaaa aatttttagg
    49261 aatgatgttt catttcgtgt ggaatctcca agcatatcct tttgatttcc aactaaaaaa
    49321 cttttttgtg tggctggact tgcttggcta tccaatccac tggctggaac agatttcaaa
    49381 gatggagtat ttggaaaaag ttcttttttt ggcagaaaaa agcaaaaaaa tacttgtttt
    49441 agaaaaaata ggtataatag acggatacca acatccaaac gaaaaagaat tattgagaac
    49501 aatgacggta tttcaacaaa tcgtcagtga gataagaggg aattgaaatg aatacaaata
    49561 gcgaaaaaat gttgcaagct ttatcagatg aagacttgtc acaagcacaa atctttttag
    49621 aaaaagcatt aaaagaagac ccagcagata tattagctga actaggcgaa gaattgctgg
    49681 ctattggctt tttgatcgaa gcaaaacaga tttttgaaca actgatcaac caatacccag
    49741 aaaatgacgg actgaacatc ccgttagcag aaattgcaat cgaagataat gagatcgact
    49801 tagcttttga atacctggaa aaagtggata agcaaagtga ttactatccg caaagcttat
    49861 tggcgatagc tgatttgtac caagttatcg gaatccctga agtgagtgaa gcaaaattga
    49921 aagaagcagc tgggttgctt cctgatgaac cattgatcca gtttgcgcta gctgaattgt
    49981 atttttcagt tgatcgtttc aaagaggcag cagtggttta tgaaacattg ttggcaagcg
    50041 ggataaatga aatttcagga atctcgatgc aggaacgttt aggacaatcc ttaagtatgc
    50101 aaggagaatt cgaagcagct atcgtacctt tagaagaagc actgaaagaa gaaaggacag
    50161 atgatcgact gttccagcta gcttttacga atcttcaatt gaaagaaaat cagcaggcaa
    50221 tcaactatct ggaagaactt cgagaagtta atccgcatta tcaatcgtta tatctatatc
    50281 taggacaagc tcttcaagaa gaagaaatga ttgaagaagc acaaacagtg ttagaagaag
    50341 gaatcaaaga aaatccttac caagtcgaac tttatcacct ggcctcggaa aatgctttcc
    50401 gtctgcatga tagacagcga gcagaagaac ttttactgaa agctttagaa gtaggagaca
    50461 aacaagatga aacattactt actttaagta atctttactt ggatgatgaa agatatgaag
    50521 atgtgataca agtgatcaat cagatggaag aaactgctaa tccttatgca gaatggaacc
    50581 tagcccatgc ttataatgaa ttagaagact ttaccgttgc agctgtacac tatgaacagg
    50641 cttatcatga actatcacat gaaccagatt tcttgaaaga gtatgccttt ttcttaagag
    50701 aagaagggca gctgaaaaga gcgcaagaat tattgactta ttatttaaca cttgaaccag
    50761 gagatatgga ggcactgtca ttattagatg acctaacaga gaggtgagaa aaatgctaat
    50821 tgatgtacgg gaaaagaaga attttttaac gtggatggtc aatcatgtat cttttagccg
    50881 aagagaagtt ttttggatct tgaattatct tgccaatcat gaagcgattt taagcaatgt
    50941 ccactttgtt gaaggggcga attttgcgga tcgaggactg cagatcagtg atctatctgt
    51001 tgaaggggaa cccatgaaat tgtttttgca gggaaaagaa tttacggaca cagaccagat
    51061 ttttcatgaa atccgtttga attggaaaaa gcctttatat gtggaatgta tattcgaaaa
    51121 tgcttggcag acaagagaat atctatctat cttagaagac aatccatttt cttcttggga
    51181 ttcttcagtc agtgaagaag atactgaatt aattgaacga tattttcaag aaaaagaaga
    51241 agaggcaaaa ctaaaaatgc tttatgctca aatcgatcaa gcactagaag atggagatag
    51301 ggatgctttt ttgaaattat ctgatgaagt taaccgaaag atccttttgc agacacaaaa
    51361 gaaaagtaac cgggtaagtg aacataacaa ttagttaaaa atagtatagt actaaaaaag
    51421 acaaaggtca gctggatgtg gaatctttcc tcatagccta tctttgtctt ttttcttata
    51481 gttttttcag taatcttgtc tgtggcttca aataagtaaa gccttgccct tccacttcat
    51541 gcacattgat gattgaaagg aaagcttttt cgtcaatctc gtggacgatc ctcttagctt
    51601 cagttaattc actagagcta acgaccatat agatgatttt ttttgaatta ccagagaatg
    51661 cgccttctcc agaaaggaaa gtcacacctc tttgcagacc ggtcatcagt agtggtgcaa
    51721 tctcttcgct tttgttggag acgacaagta gaccttttgc agaatatccg ccatctaaga
    51781 tcgaatcaat cacacgactg aaaacatacg aaacaatcaa cgtatacatc atacgtttca
    51841 agtcgatata ggttaatgaa gcaagtaaga cgatgatatc aaatgccaat agggaacgcc
    51901 ccatagtgat gccgtaattt tgttctaaaa tccgagcgat aatatcactg ccgcctgttg
    51961 ttccacccat gcggtagacg atcccgcttc ctacacctgc aactaaaccc gcaagtagag
    52021 aaacgatcag tagatcatgc tgtaaattga tttctagtgg aaccctttgc cagatccata
    52081 aaaagaatga caaagaaagt gtacctagaa cagtataggc caaggaacgt ttaccgagga
    52141 tctttcctcc tagtaaaatc aatgggatgt tgattaataa agtcgtataa gctggatcaa
    52201 taccaaataa agctcttaag atcaaggtga tccctgagac tcccccttct gctaaatgat
    52261 tagcgatgtt caagtaaaca agaccaaatg cataaatact tgttcctata atgataaaaa
    52321 gaacttcttt tactgttttt cgtttgacca tatgaattcc agctttcaat tatgtatttt
    52381 cacagaattt ccatttagaa attccttcct aaatgtagca tggaaattcg attttctcaa
    52441 ttgctcttat ctaggatttg cgttaaagtt ttggtaagat agttttggag ggaaaaagat
    52501 ggataaaaag cgttctttat acagtatgca gcaggaagtg gatgaatata tccagcaatt
    52561 caaggtgggg tatttctctc cattagctca gatggctcga ttaactgaag aagtaggtga
    52621 attggcaaga gaagtcaacc acacttatgg agaaaaaagc aaaaaggctt ctgaaccagt
    52681 taactcggta gcagaagaac ttggtgatgt tttattcgtt acgatgatca tggctaattc
    52741 attgaatatc gacttaactg aagtttttga aaaaaacatg gaaaaattca atcaaagaga
    52801 ccataatcgc tttgagcgta aggacgagta aacaagatga agtggtcctg gccttgaaga
    52861 ctctgaaacg gacttgtcaa acagtcacca tgcaactgag tctttatctc aggacaagaa
    52921 acacgcagga aaggttggaa aaatgaaatt aaaacaatta cctctggaat accaaaaggc
    52981 aattccagtt ctgaaaaaat tagagaatgc aggatatgaa gcttattttg taggtggaag
    53041 tgttcgtgat atcttgctga accagccaat ccatgatgtg gatatcgcaa caagcgcatt
    53101 tccagaagaa atcaaacagc tttttccaaa aacgatagat attgggatcg aacatggaac
    53161 tgtgttggta ttagaaaatg atgaacaata tgagatcacc acatttcgga cggaatcaac
    53221 ttatcaggac taccgtagac cagatcatgt cgaattcgtt cgttcgttgg cagaagattt
    53281 gaaaagaaga gattttacga tcaatgcgtt tgctttgaaa gaagatggag aaatcatcga
    53341 tttgttcgat ggtctgcaag atctagaaga acagatcttg cgagctgtag gcaatcctca
    53401 tgagcggttc catgaagatg ccttacgaat gatgcgcggg ttgcgttttg tcagtcaact
    53461 agggttcaaa ttagaggaag aaaccttcta cgccatcaaa gaaaaccacg agctattagg
    53521 taaaatatct gtagaacgta taaatgtgga attcatcaag atgctcttgg gaaatgaacg
    53581 ggccgcggga atccatgcat ttattgaaac agagtgctac atttgctgtc ccggtttgaa
    53641 agaagaaggc gaagcacttt tacgttttgc tgaattacca tccgtcccat tgaaagaaga
    53701 gagccaagca tgggcattat tgatcagcca actggatcta aaagaaaatg agattcgttc
    53761 ctttttgaaa tcgtggaagt gttctaacca aatgattaaa gacgttcaag cacttgtcca
    53821 aggactacga caacgagaaa ctggagcttt ggaaccattc gaattgtact tcttaggcaa
    53881 aaaacatgca ctgcaagtag aagaattgat gatctatttt gacgaaacac ctcagatgga
    53941 tgccgtagga gaatcctatg atcgtttacc tatcaagtca ttaagcgaat tagcagtaga
    54001 tggtaaaatc ttgctagaag aaactggaaa aaaacctggt aagtgggtta gtgaagcttt
    54061 gcaattagct gaaaaagccg tcgtagaaca aagaataaaa aatgataaaa aacaggtttt
    54121 ggatttttta tcgaaagaaa aattgattta aaaaacacta gactgtatct gctcagcttt
    54181 gttggctact taaagtgggc agatacgtca ttagaagaaa aaacataaga tgtgaccact
    54241 tacataaatt tgtgctaaac tgaggtagta caagaataaa ttacatcata aaggagtctt
    54301 tttaatgagt aaagaaatga cagcgctaaa attttatttt agaaatggag aaacctggac
    54361 gatcaaccgc cgtcatatcg gcgatctttg gatcaaacaa atcacaacaa gttttggacg
    54421 aatcaatggc agtgaatttg ttgaaatcca tccgtgtgct ggatttaaaa tcgaaatctt
    54481 ccatgaaggg gacgcagttg cgacacatga tatcaaccta ggcggactgg aaatgggcat
    54541 gtttaatcgc gcattgaaat acgaagatat cgagcgaatg gaaatccttt accgaaatgg
    54601 cacacctgat cttgtgtact tcccatatct agataaagga acagaaggat tagacaatca
    54661 atatcaatca acaaaaatca gcgaaaaaac aggtaacttg tatatcgtga tcaatccaga
    54721 tcaacgtgta gaagatgtat acggggaatt tttcgaataa atcgattttt acgcaaaaat
    54781 gaggctgtga aagagatgtg tcttattact actgataggc tgtctcttcc acaccgttta
    54841 ttcagaataa gggactgtga caagattttt gtcgcagccc catttttttg ttgtgataaa
    54901 agaacaagaa aggcaggaat gggaaagtaa acatcctttt gtcagaaact tttatctttc
    54961 ttaagtttta cttgatatct gatactattt ggaatagaat gaggcgttga aaaatttatg
    55021 gaaatttcta actagcaata aacgatgaaa caatgaaata tttttcatcc aaaaagaact
    55081 ttgctataat gatggaagtt tgtgtatagg agatctggaa aatgagagag agttttgaac
    55141 aacaaaaaaa actgttgcat gatcgctatg gcgccctttc gatggatgac cgccgtcaaa
    55201 tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa cgtctgaaac
    55261 atgatctgct tcgtttggag tcaaaacgtg tacagtgtga attagagggg aaccaaacac
    55321 aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga acaattttta aaaatgctta
    55381 cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt attgtatttg
    55441 cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt ccagctccac
    55501 tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa tcaatcgatt
    55561 ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga gaaggagaaa
    55621 aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg gcatttggag
    55681 gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg ccaagtgtcc
    55741 cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg gtagcagtca
    55801 gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg gaaggagaag
    55861 gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt gcgctgatca
    55921 ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt attgggggag
    55981 ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg atcgaaaaag
    56041 catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct tttctagcct
    56101 atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca gcaggtattc
    56161 tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg agcttgtccc
    56221 acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata tttttaggaa
    56281 tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca aacggtcttt
    56341 tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc atttctatca
    56401 gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg aacgagatct
    56461 tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt tttattttgc
    56521 cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt ctgacggcgg
    56581 gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg acggaaacag
    56641 aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc gttgaagttt
    56701 tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc actgaagcag
    56761 tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta accgatgatg
    56821 aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg gatggattgg
    56881 atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac tcccgttttc
    56941 tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc ttttggttca
    57001 ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg caaagaagat
    57061 atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga gagacatatc
    57121 aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt tatgatgaaa
    57181 cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg aactcgggga
    57241 atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa atgctaagag
    57301 gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa atcacgacgt
    57361 tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg atgaacaaac
    57421 catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa acgagtccaa
    57481 taaaataaaa aggtaaaaat tttcaagttt tataaaaaac aaaacatatc agaacaggat
    57541 cgaatcggtc ctgttctttt tttgagaaca caaaaagaac aaataccaaa aaaattttcc
    57601 tgaagttcac tatttttatg atacaatatc caagataagg agggaacatg ttgaaagaat
    57661 taaaagtaac tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac cagatttctt
    57721 ttttggtcca cgaaaaagac cgtatcgggt tgattgggac gaacgggacg ggtaaaacaa
    57781 gtctcttaaa tattattgcc ggtatcgata gcagcgacgg tgatcggcaa acagtgtttt
    57841 atccaactga ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc gatgaattaa
    57901 ctgttctcca agcagtcttt caaggaaaca gccccttgat ccaaactgtt cgagcatatg
    57961 aggaagcatt gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa aaacgttatg
    58021 ctaaagcaga agaacaaatg aataaagaag atgcttggac tacagatacc aacgcaaaga
    58081 ttattttgca aaaattagga atcagtgaat tagataaaaa aatcagtact ttatcaggtg
    58141 gtcagaaaaa acgagttagt ctggcgcaag tgttgattga tgaacccgat ttactattac
    58201 tagacgaacc aacaaaccat ttagattatg aagcgatcga gtggttggaa aactatttga
    58261 aacaataccg tggcgcacta ttgatggtta cccacgatcg ttattttcta gatagagtag
    58321 caaaccgaat cttcgaactt tcatttggaa aattatacga gtataaagga aattatgaag
    58381 cttacgtctt agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa gaaaagcgca
    58441 agcggcttta taaacaggaa ttagcttgga tgagggctgg cgtacaggca cgtggcacca
    58501 agcagcaagc acggatcaac cgtttcgaag atctaaagga gaacttgtac caagtaaacc
    58561 aagaagatga tttagagctg aacctagcga cacaacgttt agggaaaaaa gtactggaaa
    58621 tcaaagatgg cagttaccgt ataaatgacc aaacactgct agaacattta gatttattga
    58681 ttcaatctcg ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa tcgacattgc
    58741 ttaatattct agctggcaga attcccctgg atagcggtac aatgtctatc ggtgaaacgg
    58801 ttcatcttgc gtattataca caagaaaatg aagaaatggc accagataaa cggatgattg
    58861 cttacttaca agaagctgct gaagaagcaa aaacagcaga tggcagtcaa atcggagtag
    58921 cagaattact tgaacgattt ttatttccaa gatttatgca tgggacactg ataggcaaac
    58981 tttcaggcgg agaaaaacgc cggttatttt tgttgaaact gctgattcag caacctaatg
    59041 ttttattatt ggatgaaccg accaatgatt tggacatagc aacattgacg attttggaag
    59101 attattttag aagttttcca ggtgctgtga tcactgtttc ccatgaccgt tatttcttgg
    59161 acaaggtagc agataagtta ttagtattcc aaggaaacgg aaaacaagaa ctttattacg
    59221 gaaacatgtc cagttattta ctgaagcaaa aagagaccca gcagcctgct gaaaaagcga
    59281 aaccaaaaat acagagcaag gaacctgctg aaaagaagaa attatcttac atggaacaaa
    59341 aagaatggga aacgattgaa gatgagattg cagaacttga agaaaaaatc tcattgctcc
    59401 aagaagagat gaatcatcaa ggagataatt tcactcgatt acaagaattg caaaacgacg
    59461 tatctgaaac agaagcacaa cttgaagaaa aaatggcacg gtgggaatac ttgagtgaat
    59521 gggtagaaga ttaggaggaa aaaggatgga acaagcgtat cttgacttag gaagaaaatt
    59581 gttggaagaa gggcatcgta aagaagacag aacaggcaca ggcacattaa gcttattcgg
    59641 ataccaaatg cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga aacgagtacc
    59701 ttttggattg atcaaaagcg aattgctttg gtttttaaaa ggagatacga atatccgcta
    59761 tctattacaa aataaaaacc atatttggga tgaatgggca tttgaacgct acatcaaaag
    59821 tccagactat aatggaccag atatgactga ttttggccat agatgtttga cagatcctga
    59881 attcaatgaa gaatataaaa agcagagcaa gcttttttgc gagaagatcc taaccgatga
    59941 ttcctttgct gaaacatacg gcgatcttgg acacatctac ggctatcaat ggcgtcactg
    60001 ggaaacaaaa gacggtggct ttatcgatca aatcaaacaa gtgatcgaag caatcaaaaa
    60061 gactcctgat tctagaagat tgatcgtttc tgcatggaat ccggaagatg ttcctagcat
    60121 ggctttgcct ccttgtcata cgatgtttca attttatgtt caagagggac ggctaagctg
    60181 ccaattgtat caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca ttgcaagtta
    60241 tgcgttgctg actcacctaa ttgcacatga aacaggttta gcggttggcg aatttgttca
    60301 cacattagga gatgctcatc tttatatcaa tcatatcgaa caaatgaaag aacaattgag
    60361 ccgagaaata cgcgcatttc cgactttagt tttgaatcag gaaaagcagt cagtatttga
    60421 ttttgagatg gaagatatcc aattagaagg atatgaccca catccggcga tcaaagctcc
    60481 tatcgctgtt tagaatcaga aaggatgaga aaatgttagc tgcaatttgg gcacaggatg
    60541 aaaacggatt gattggaaaa gaagaccgtc ttccttggcg tttgccaaat gatctgaaat
    60601 tcttcaaaca aatgacagaa gcaaatacac tagtgatggg acgaaaaaca ttcgaaggga
    60661 tgggtagccg tccgttgccg aatcgtcaga cgattgtttt gacgagagat tcttcctata
    60721 aagcagaagg cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg gaaaaagaaa
    60781 cagacggtat ctttttcatc gctggagggt cagctgttta tcaagaattt ctgccatttt
    60841 gtacgatatt ataccgtaca gtcatccatc atgtattcga tggagatgct tattttccac
    60901 ctgttgactg gtctgattgg tcactgatca atactagtca aggagaaatg gatgaaaaaa
    60961 atcgttatcc gcatcaattt gaaacgtatc agcgtaatga gaatcctgta aaaagcggga
    61021 caggcgaatt gacgatataa aaaagctatg gatagactag ctgttattta gagctgtccc
    61081 atccatagct ttattttttt tattaagtaa agaataaaac agagaagtac ataaaacctg
    61141 cacctaacat aacaaacaaa tgccaaacaa catgcatata gcggatagaa ttcaagctat
    61201 aaaagaatgc gcctatcgtg taagaaagcc cgcctgctac catcaaaata acaccagtca
    61261 ttccaagaga gtgataaaga ggaacagcag ccacgacaca gagccacccc ataaaaatat
    61321 agatgatcgt agatactttt gatactctct cccgtttatg caacgtcaac gctttatata
    61381 taatgccaag aatcgcaaac aaccagatta atacaaataa agtccaacct agccatcctc
    61441 ggatactcag caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa atcgaactat
    61501 gatcgaatac ttggaacacc ttttttgctt ttgtaaaaat caatgaatga aacaaagtag
    61561 acgttaaaaa taaaagaatc atcatcgatc catagatagc ataagagaca atatggaggg
    61621 aggaatgcaa gtgggcacct tttactaaaa gaaccactag tcccgcgata ctcaatccag
    61681 tcccaattcc atgggtgact gcattaaaca cttcatttaa tatttcatac gttcgattaa
    61741 atttcgtttt ttccatgaaa acggctcctt tactaggtgt aattaatgaa aaatcattat
    61801 ttgtctgtta tactaaaaat agaaattgtc ttacatgtag tttaacatga ataaggcatc
    61861 ttgttaaaga aagagagaga gaaaataatg acaaatgtaa aaatcgtaac agattcttca
    61921 tgtacgatgg aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc gctatccatc
    61981 atgatcgacg gaacagtata tcccgatgat gaccaactag acggcgaaag atttatggaa
    62041 atgatggcga aagcaaaaaa tctgcccaaa acaagccaac caccaattgg agaattcatc
    62101 tcgatgtatg atgagctagg agaagacggc agtgaagtga tttcgatcca tatgacaaaa
    62161 ggcttgagtg gaacagtaga agcagcaaga caagccagta atctaacaaa aacgaaagta
    62221 acggttatcg acagtgatac cactgaccaa ggattatctt ttcaggtaat tcgagcagct
    62281 gaattagcaa aagaaggtaa aacagtagag aaaatccttg aagaagtcaa aaaagtacaa
    62341 gacaatacaa aattatacat cggaatatcc acactagata acttggtaaa aggtggaaga
    62401 atcagccgaa caactggttt gctgtctagt gttttgaaca tacgcgtggt gatgaatttc
    62461 gcccattctg aattaatccc tgtaactaag gggcgaggaa agaaaacgtt cgataaatgg
    62521 tttaataatt tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat cggtatttca
    62581 catgcggatg caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga attattcccg
    62641 gacatgcata tccctgtact gcatacgaat ccggttatcg ctacgcatac tggtaaagac
    62701 gcgtttgcaa tcatgtatta tgtagattag tcgaaaagag gataagccct cagcctgaaa
    62761 ggcttgcagc ttattctctt tttctttata aaattaggaa aataattgtt ttcttttttc
    62821 ataaagacta cctttttgat aaaatttttg ataacattag aaccaatcga atacaagagg
    62881 tgaaaaaatg aaattaacga aacgacattt gatcctcgtt ttgttcccat tattaattgc
    62941 gatcatcatc tatgcaggtt tagctgcttc tataccgaaa gctggcccac taatgaaacc
    63001 agtaactact gtgagagaag atccaaataa aaaagaaaaa cttcactaca tagctatcgg
    63061 agattcgctg actgaaggag taggagatca aaccaagcaa ggaggattcg ttccccttgt
    63121 cgcaaatgat ctaaaagacc gttataattt gaccgcggtt gaaacagaaa actatggtgt
    63181 aaatggtgaa cgaagtgacc agatcttaaa gcgagtaaaa acaaaagaag aaattcgaaa
    63241 caatatcaaa acagctgatt ttatcacact gactgttggc ggtaatgatt tgatgaaagt
    63301 tatccaaaat aatttattcg ggttaacggt caattccttt aaaaagccac tgaaaaacta
    63361 tcaggaaaat gtcacagaac tattggaaga atttcgatcc ctcaataaaa ccgcacctat
    63421 ttacatttta ggcatctaca atccatttta tttgaacttt cctgagatca ctgacatgca
    63481 gacaattgtt aacaattgga acaaaggaac agaaacggtg acaaaaaaat gccgaactgc
    63541 tactttatcc caatcaatga tttgctttat caaggattga atcaggaagt aggtgtcaca
    63601 gaagaaggaa cagatcaaac aactagcacg acagaaaatc taaatggcat caaaaataat
    63661 gtgctctatg aagaagatca tttccatcca aataatttag gttatcaatt gatggcaaat
    63721 gcagtgagag atcagctgat cgagacacaa aaccaatggt tagagaagga ggatgatcaa
    63781 taaacatgaa aacaagatct gacgtatctt caaacaaaac aaagaaaaca aattactgga
    63841 aattcgcctt ccttatcctt cttggggtag tacttggttc gaccgtattt ttaggcagcc
    63901 gtatttttgc taatcgggaa ccagaactcc cagaaatacc agctcttacg gaaagacaag
    63961 gagatcctgt cttaacgatc aattcaaata aagaaaaagt caatcaaatc atcagctttt
    64021 tccttagtga gtatcaaaaa gatagtgata ttgaatataa attttatttg gaaaatgaag
    64081 cactactcaa tggtactttc gaagtactag gttttcctat cgatttctat ttatattttg
    64141 atccgtatgt gatggaaaac ggaaatgtcc aactaaaagc tagaagtctt tctatcggaa
    64201 cattgagttt gccaatcaaa gatgtgatga atatgatcaa gagaaactat aaattaccag
    64261 attggatcga agtcaataca gatgatctga ctatcatgct taggttggat caattccgaa
    64321 tgcaaaacgg gatgtatatt aaagcagata aaatcgactt agtaaatgat gacattcggt
    64381 ttagtctata tttgccaaaa gatacctctg atgcttcaaa caactcaaat accaacaaaa
    64441 ctagcaccag cagtactgaa agcaagacaa aagaaaataa aaaatcatcg gatgagtagt
    64501 attagagggg gaatcataat ggaacgagca atatttgcag gaggctgctt ctggtgtatg
    64561 atccagcctt tcgacacgct tcctggtatc catacagtga tgtcaggata tactggaggg
    64621 catgtaccag atccgactta tgaacaagta aaaagccaaa cgactggaca tacagaagca
    64681 gtggagatta tctatgatcc aacattgatt tcatatgaac aattagtaga actttactgg
    64741 cagcaaactg atccaactga cgctttcgga caattcgagg atcgcggtga caattatcgc
    64801 ccggtgatct tttatttgac agaagaacag cggatcattg ctgaaaaaag taagaaacag
    64861 ttagctgaaa gtggacgttt cgaacagcca atcgttacaa aaatcgaacc agcccaacca
    64921 ttttatctag cagaagaaga ccatcaagag tattataaaa agcatcctga aaattttaaa
    64981 agaaaccatg cacgccgggc tgcctttatc gaagaaaatt ggaggaacga agggtgaatc
    65041 gaagcttttt tcattattta atgacactgc gaggcggtgg cacacatgat gctttatctg
    65101 ttttcgctac agaagccggt aaagacatcc aatttcctaa gcacagctct agctacgaag
    65161 aaatttctaa ttatctggaa ctgaatgtcg actatttgcc gtccatggat atctttgacg
    65221 aagcatggga aaaatattta gaaaataatc atgacatata agagatagtt ttgtacttta
    65281 tgatcaaaaa agttttttgt ccaatagcct aattgaaaga attattttta tttcagctat
    65341 ctagtcgcat ttttttgata tttttcttat aataaagaaa gaatgaaaaa aagagggatg
    65401 gattgataga gattgacaaa aaaacaacgc tatattaatc gcttctggct tgtattcaaa
    65461 tacttacttg tttctgtatt ccttgcgttc atgttgcggg gatttttatt catcccggta
    65521 ccagtggaag gcaattcaat ggaaaacgtg ttgaagcaag gtgatatggt tgtgatggaa
    65581 aaattttcgg agatcagacg atttgatatt gttgtttttc aattagcaga tggcacgatt
    65641 tatatcaaac gagtaatcgg tcttccagga gaaaatgtca gctaccaaaa tgatcaattg
    65701 aagatcaatg ggaaagtagt taaggaacct tatctaacta aaaacctgaa atcagatcat
    65761 gcaaatgcat cctatacgac ggactttaca ctacaagagc taacgggaca atccaagctt
    65821 ccggaagata attattttgt acttggagat aatcgaagag tttcaaaaga tagtcgttcg
    65881 tttggcacga tcaataaaac tgatattcta ggtaaagcaa gatttgtcta ttatccactt
    65941 gatgaaataa aatggatcca gtgagaaaca ataaagcgtg cagtctgttg agactgcacg
    66001 ctttattgtt taatccaaaa tcagtttact accttagtac gtaaaatcaa acagctcgtt
    66061 taaacacttt tctagcctct aaagttttga taaatgaata aacaaccatt atttgaatag
    66121 ggatttggat catattcctg atgaatctac ctagaaaaat tactttgaaa tctgcaccaa
    66181 acaaaacaga catcagccaa gaattgataa aaaggctgac tgggatcata atcaataatt
    66241 gggcagcgat gacggaaggc aggtttatct ttttatgata taaaaataag ccattaataa
    66301 atccaccaag gaaagccgtg ataagaaaag cagcttgaaa cgttcctgaa ccaaatatcg
    66361 tgaaatttaa agtgttcgac aacatttcaa gcaaaccagc ccagatcggt ccatatagat
    66421 aacccataag cgccatactt aaaaatccga atgtgatttg aaaataagca ggaccaatcg
    66481 ctatcttgta taagatgaca tcaagggcta ataaaagagc agaggtcgac aacaatcttg
    66541 cattcatttc gggcaatcta aaaacgtctt ttgcaatcaa agtgttcaaa atattctctc
    66601 ctttgtcagt taataataaa aggataccat aaaatcacca atgtgtcatg acacattggt
    66661 gatttacata cactggcatc taacaattaa aaaaagggtg agaagactgc ttttataaac
    66721 tgtaaaaaag agattgtacg aataaaaaaa atagaggcgg agaatactta tcgtatcatc
    66781 cgcctttgtt tttttgttat aaaaaacgtc agattagtta tctgcttaca tcttcatcca
    66841 tacgggttct aatgtatttc cgtctagatc ttggacttct aatgaaaaca tttcttcttc
    66901 aggcattccc atatctactt cgtggaaagt accaccattt tcttttgcaa tttgagcaaa
    66961 tttttttact tcatccgcat tttcaacact aaacgaaatc aaagcaccac ttgttttttt
    67021 attatcagct atttctttat cttttataaa ttttttataa aaatcatgag agagaagcat
    67081 tatccaaaaa gtgtcatccc ataccattga accagcttct tcagtagaaa attcttcatt
    67141 ttttttaaaa cccagctttt cataaaaagc aacggaacga ttgacatcag caacaggaaa
    67201 attaacaaaa accatactat tcatttgttt cgtctccttt ctgttttgtt cgtctatcat
    67261 ctcttattta ggaaacgctg tcaaaaattt agattcatat atagaaacca aaataaaaat
    67321 ttgcatcatc aaaaaagtga agatgtgata gaatgaggac tggttcactt tcacttaact
    67381 aatagatgga atttctttat aataaaaaag tgaatttata ttgaaaaaga aaaaaacaag
    67441 taaagccggg gaatattccg gtatgaaagg aatggagaga acaatgacca tccaatggtt
    67501 tccgggacat atggcaaaag cccgacgaga agtttctgaa aaaatgaaat atgtggacat
    67561 cgtatttgaa ttggtagacg cacgattacc tttatcttct cgtaatccaa tgctagatca
    67621 gatcattcag caaaaacctc gccttgtttt attgaataaa gcggatctgg cagatccaca
    67681 acagacaaag ctgtggcaag aatattttcg taaacaaggc catcttgcat tagctatcac
    67741 ggcacaagaa agtaaaggaa ttaaaggtct agtacctgcg gctaaagaag cattgaaaga
    67801 aaaacgggca agagatgctg ctcgggggat caagcctcgc gctattcggg caatggtctt
    67861 aggtattcca aatgttggga aatctacgtt gatgaatcga ctagtaggca agaaaattgc
    67921 acagacagga aataagcctg gcgtaacaaa aggtcagcag tggcttcgtt taggtaatga
    67981 gttggaattg ttagacacac cagggatttt atggcccaaa tttgaagatg aagaaatcgg
    68041 caaaaaacta gcgctgacag gcgcgatcaa agaccagctt gtccatttag atgatttagc
    68101 gatttacgga ttagactttt ttgcccgata ttattcagga agaatcagcg atcgttatgg
    68161 tttatcaatc gagcaagaag aaaaattggc accggaatta ctgatggata ttacagcaaa
    68221 acgtgggtat aaagaagact atgatcgtgg cagcgaatta gtcgtgcatg atattcgtca
    68281 aggaaaactt ggacgataca cactggatcg ttgcgaagaa atggagcaaa aaagtgatga
    68341 ctgaatccat ccaaagtatc aaagaacgat tgaaaagcat acagagaata gatgatccta
    68401 cactagaatc attaagaaat gatccacgca aaggcgtgca gcaagcactg gcttcttttg
    68461 aacgacgatt gaaaaaggaa gcattaatta aggaaaaata tgaagaaatg ttttgctttg
    68521 aaaagcaagg gtacgcacaa ggtcaccaat ggattgccgg cattgatgaa gtaggaagag
    68581 gaccattagc tggacctgtc gtggcagctg cagttatctt gccgaaaaat gagatcatat
    68641 tagggctgaa tgactccaaa caattatcag agaaaaagcg agaacttctt tatcaacaag
    68701 ttcaagaaaa agccctggca atcgggatcg ggatcgttga tcagcagaca atcgatgatg
    68761 tgaatattta tgaagcaagt aaattagcga tgatccaagc agtagaaaaa ttgtccattg
    68821 taccaagcta tttgttgatc gatgcgatga ccttggattt gcctattcag caggaaaaaa
    68881 taatcaaagg ggatgcacga tctgtgtcga ttgctgctgc aagtatcatt gctaaagtat
    68941 atcgagacca attgatgaaa gaatatgacg tgctttatcc tggatatggt ttctcgcata
    69001 atgcaggtta tggaacaaag gaacacttag aaggacttga acgacaagga attacgccaa
    69061 tccatcgtaa aagttttgca cctgtaaaaa aatatttaca ataaaaaaca gccgctttta
    69121 cgtatcttat tagaaagaga gacgaaaggc ggttttatga tgtatcaaat agaagaaaat
    69181 ttattgaaac ttgcttatac aaaaggaatc agtagtcaag gaaaatgggc acttgctaac
    69241 tggatgatgc agtatcctta taaagaaatc ggatttgaag acatcattaa gataggtaaa
    69301 ataaccaaac accgggagct gtttcgcaaa agttggcagg aaattcacgc taactggggc
    69361 aaaatccaat cgaaacagtc gtttatcact tgctttgatc ccaaatatcc ccctcagctg
    69421 cttcacttga cctatccgcc aattgtactg ttttataatg gtgatctatc tttactttct
    69481 tgtaacatgt taagcgttgt tggtggaaga cacacttctg ctttagccaa aaaaacagtt
    69541 tattatttac taaaaccagt gatagaagct ggatatgtga tcgtcagtgg ctgcgcgaaa
    69601 gggatagata catacggtca ccaagcagca atctctcatg gtggacggac aatcgcagtc
    69661 attggaacag ggatagagca agtctatcct gctgaaaatc gtgacttgca agaggtgatt
    69721 gcaaatgatc atcttctttt gagtgaatat cctcctcaat ccggcccgaa aaagcattgt
    69781 tttccaatga gaaatcggat tatcgcagct ttgtctaaag gaacatgtgt ctttgaagcc
    69841 aagaaaaaaa gcggctctct gataacagca cagcaagcat tggatcttgg aagaacagtt
    69901 ttttctgttc caggaaatat tttaacagaa cattctaccg gatgtcatca tttgatccaa
    69961 gacggtgcag tttgcacggt ttcgggtgaa gatatcttgg cagaatttaa agaataacac
    70021 catacttttc atttgctttt cttgacaaac gttttctgaa tggatatgat tagctacgat
    70081 ttgtatgaat cctcttttaa tagtaggaac acgacaatgg aaaggagtcc attcagtaat
    70141 ggcgtataaa tatttagtga ttgtagaatc acctgcaaaa gccaagacaa ttgagaaata
    70201 tcttggacga aactataaag taatggccag tgttggccat ataagagatt taccgaaaag
    70261 taaaatgggt atcgattttg aaaacaatta tgaaccccat tatatttcta tacgcggaaa
    70321 aggcgatgtc atcaaaagcc tgaaagccgc agcaaaaaaa gctcaaaaag tttacttggc
    70381 aagtgacccg gatagagaag gagaagcgat tgcttggcat ttagcgtacc tacttgggtt
    70441 ggatctgaaa gaaaaaaatc gggtggtctt caatgaaatc acaaaagacg cagtcaaagc
    70501 agcttttaag gaaccaagaa cgatcgatgt agatttagta gatgcacagc aagctcgtcg
    70561 taccttagac agaatcgttg gttattcgat cagtcctatt ctctggcgta aggtcaagaa
    70621 agggttaagt gcaggacgtg tccaatctgt cgctttaaaa attattattg accgtgaaaa
    70681 agagatccga gaatttgttc cagaagaata ttggagcatc gacggtaatt ttaaaaaagc
    70741 tcgcaagaaa ttcaaagcaa atttctgggg aatcgacggt aagaaaaaga aattaccaga
    70801 tgcacaaagt gtaaaagaag tcactgctag aattgatgga acagacttcg aagttaaaaa
    70861 agtagaaaag aaagagcgca aaagaaaccc tgcgaatcct ttcactacaa gtagcctgca
    70921 acaagaagct gcccgtaagt taaactttcg aacaagaaaa accatgatgg tagcacagca
    70981 attatatgaa ggaatcactt tgggtaaaca aggaacagtc ggcttgatta catacatgcg
    71041 tactgattct acccggattg ccgatacagc aaaagcagag gccgcagcat ttattgaaga
    71101 tacgtatgga aaagaatttt ctagacatga tcaccgaaag atcaaaaatg ctcaaggcgc
    71161 acaagatgcc catgaagctg tgcgtccaac gagtgtgctt cgtacaccag atgaactcaa
    71221 acaatattta gataaagatc aattgaaact ttatacattg atctggtcac gttttgtggc
    71281 tagtcagatg acaccagcta ttttagatac aatgaaagtg acattagaac aaaatggtgt
    71341 catcttcatt gcgaacggtt caaaaatcaa attcaaagga ttcatgcaag tgtatgtgga
    71401 aggtagagat gacggcaaag aagagaaaga aaatattctt ccagaacttg aagaaggcga
    71461 tgtagtccaa tctgttgata ttgaaccaaa acaacatttt actcaaccac cagcacgttt
    71521 tagtgaggct acgttgattc gtgcactaga agaaaacggg gtagggcgac cttccactta
    71581 tgcaccaaca cttgatacga tccaaagacg ctactatgta aaattgacac aaaaaagatt
    71641 tgagccgact gaactaggag aaattgtcaa ttcattaatc tgtgaattct tccctcaaat
    71701 cgtcgatatc catttcacag cagaaatgga aggagatttg gataaaattg aagaaggcac
    71761 ggaagcgtgg gtcaaagtgg ttgatcgttt ctataaaccg tttgaaaaag aactgacgaa
    71821 tgcggaagaa aaaatcgaaa aaattcaaat caaagatgaa ccagcaggat tcgattgtga
    71881 tgtctgtgga catccgatgg tgataaaatt agggaaatat gggaagtttt atgcttgcag
    71941 taattttcct gattgtcgga atacgaaacc aatcgtaaaa gaaatcggtg tgacttgccc
    72001 agtatgtcat gaaggacaag tcattgaacg aaaatctaag aaaaacagac tattctatgg
    72061 ttgttcccgt ttcccagaat gtgaattcac ttcttgggat aaaccagtgg gcagaaattg
    72121 tccaaaatgt gacggatatc tggtcgagaa gaaagtcaaa ggcggtaaac aagtagtctg
    72181 tgtaaatgga gattacgaag aagacatcca aaaataatag aaaagaggtt ctttcattga
    72241 aaatggtaaa tgttgttgga gcagggcttg ccggaagtga agctgcttgg cagatcgcac
    72301 aagcaggagt ccctgtccgt ttatatgaaa tgcgtccagt caaatcgact gaggcgcatc
    72361 acacaagtaa ttttgctgaa ctagtatgtt ccaattcatt aagaggaaac agtttggcta
    72421 atgcagtagg gttattgaaa gaagaaatgc gccgattgaa ttcggtagta atcacttcgg
    72481 cagatgaaac agcagtgcca gctggtggtg ccttagctgt tgaccgcgat tctttttctg
    72541 aaacaattac ccgaaaagtc aaagagcatc cattagtaac agtgataaac gaagaaatta
    72601 ctgaaatacc ggaaggaatc accgtagtag caacaggacc tttgacttct catccattag
    72661 cagaaagcat caaagcattc aatggttcag atggttttta tttctatgat gcagctgcac
    72721 caatcgtaga caaagctacg atcgatatgg ataaagtata tttgaagtcc cgctatgaca
    72781 aaggagaagc agcttacttg aactgtccga tgacagaaga ggaattcaaa gctttccatg
    72841 aggcactaat caatgccgaa gtggcagaac ttaaatcttt tgagaaagaa aaatattttg
    72901 aaggatgtat gccaattgaa gtaatggcac agcgaggatt caaaacgatg cttttcggac
    72961 ctatgaaacc agttggattg gaagatccta aaactggcaa acgtccttat gctgttatcc
    73021 agctgcgtca agacaatgct gcagcttctt tatacaacat cgtgggcttt cagactcact
    73081 tgaaatgggg cgagcaaaaa cgtgttttcc ggatgattcc tgggttagaa aatgctgaat
    73141 tcgttcgcta tggtgtgatg caccgaaaca gctttatgaa ttcgccagaa ttgctgaaac
    73201 cgacttacca atcaacgaaa cgcgaagatc tgttctttgc aggtcagatg actggtgtag
    73261 aaggctatgt tgaaagtgca gcgagcggac tacttgctgg aatcaacgcc gcacgcttag
    73321 caaaagaaat ggaaccagtc gagtttccac aagaaacagc tattggaagt atggcttact
    73381 acattactca tgcggaagga aaacatttcc aaccgatgaa tgccaactat ggtctgtttc
    73441 ctgaactgcc agagcgcatt cgagacaaaa aagcaagata tgaagcaatt gcacaacgag
    73501 ctttaaaagc taatgaggca ataaaaacag aattagaaaa aactgcaatc ccaaatgagt
    73561 aaggaaactc catgaagagg gtgagacgga tagtctcgct ctttttcttg cgtttttctt
    73621 aagaatttct caaacaaaat gtgaggaatt gcgccaattg tgaacaaact tagaaatatt
    73681 ggttaaattc tgacaattta tttgtctttt tataaacgct gtgctaaatt attctctggg
    73741 acaaaaggaa ggtgagaaaa tgacagaagc cacgtgggaa caaaaatttt tgcgttattt
    73801 gattgtcgaa agaggatatt cagaaaaaac gaaagaagca tatgaagaag acattctcca
    73861 tttcaaacat tttttggagg aatctggaga cgctgatctg ctgaaggcgg aatattttac
    73921 ggttcgtgtc tatttgagtg cattagctga tcatcgtcct agctatagca gaaattctat
    73981 cagtcgcaag atagccagcc tacgttcgtt ttatcagtat cttctcaaag aagaagtgat
    74041 aaaagaaaat ccattttctt acgttcatct caaaaagaaa aatttgagac tgccacgttt
    74101 tttttatgaa aatgaaatgc aagcactttt tgacagcgta gcaggagata gtcctttgga
    74161 tttgaggaac cgcgctttac tggaagtcct ttacgggtca ggcatccgtt taagtgagtg
    74221 tagcagtttg tcggtatcag acattgattt tgattcagag gtcatgttga tccatggtaa
    74281 agggaacaaa gagagatatg ctcctctagg ttcttttgct caagatgctc tgcaagaata
    74341 tttcagggaa ggccgccagg tattgatgac aaaatatcac gaagagcatg atatcgtttt
    74401 tgtgaatcat catggaaaac caatcacgcc tactggaatt gaatatgtct tgaatcaaat
    74461 gatcaaaaaa agcagtttgg atagtgatat ccatcctcac atgctgcgcc atacatttgc
    74521 tactcattta ttaaataatg gggcagattt gcggacagtc caagaattgc taggacatgc
    74581 caatttatcg actacccaga tctatgccca tgtaacaaaa gaaagtttac aaaaaaacta
    74641 tcgttctttc catccaagag cgtaaaaata aggaaaagaa caaatttagg aggaaacaaa
    74701 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
    74761 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
    74821 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
    74881 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
    74941 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
    75001 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
    75061 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
    75121 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
    75181 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
    75241 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
    75301 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
    75361 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
    75421 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
    75481 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
    75541 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
    75601 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
    75661 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
    75721 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
    75781 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
    75841 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
    75901 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
    75961 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
    76021 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
    76081 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
    76141 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
    76201 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
    76261 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
    76321 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
    76381 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
    76441 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
    76501 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
    76561 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
    76621 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
    76681 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
    76741 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
    76801 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
    76861 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
    76921 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
    76981 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
    77041 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
    77101 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
    77161 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
    77221 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
    77281 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
    77341 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
    77401 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
    77461 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
    77521 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
    77581 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
    77641 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
    77701 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
    77761 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
    77821 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
    77881 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
    77941 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
    78001 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
    78061 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
    78121 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
    78181 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
    78241 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
    78301 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
    78361 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
    78421 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
    78481 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
    78541 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
    78601 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
    78661 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
    78721 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
    78781 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
    78841 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
    78901 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
    78961 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
    79021 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
    79081 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
    79141 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
    79201 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
    79261 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
    79321 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
    79381 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
    79441 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
    79501 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
    79561 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
    79621 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
    79681 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
    79741 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
    79801 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
    79861 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
    79921 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
    79981 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
    80041 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
    80101 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
    80161 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
    80221 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
    80281 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
    80341 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
    80401 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
    80461 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
    80521 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
    80581 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
    80641 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
    80701 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
    80761 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
    80821 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
    80881 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
    80941 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
    81001 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
    81061 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
    81121 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
    81181 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
    81241 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
    81301 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
    81361 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
    81421 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
    81481 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
    81541 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
    81601 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
    81661 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
    81721 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
    81781 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
    81841 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
    81901 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
    81961 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
    82021 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
    82081 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
    82141 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
    82201 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
    82261 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
    82321 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
    82381 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
    82441 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
    82501 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
    82561 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
    82621 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
    82681 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
    82741 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
    82801 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
    82861 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
    82921 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
    82981 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
    83041 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
    83101 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
    83161 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
    83221 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
    83281 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
    83341 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
    83401 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
    83461 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
    83521 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
    83581 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
    83641 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
    83701 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
    83761 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
    83821 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
    83881 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
    83941 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
    84001 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
    84061 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
    84121 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
    84181 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
    84241 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
    84301 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
    84361 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
    84421 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
    84481 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
    84541 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
    84601 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
    84661 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    84721 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    84781 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    84841 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    84901 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    84961 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    85021 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    85081 atgaattgac acatattttc actggctggc gcaaaacaca taatcaaggt gtatttgacg
    85141 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    85201 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    85261 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    85321 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    85381 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    85441 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    85501 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    85561 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    85621 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    85681 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    85741 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    85801 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    85861 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    85921 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    85981 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    86041 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    86101 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    86161 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    86221 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    86281 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    86341 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    86401 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    86461 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    86521 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    86581 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    86641 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    86701 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    86761 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    86821 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    86881 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    86941 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    87001 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    87061 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    87121 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    87181 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    87241 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    87301 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    87361 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    87421 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    87481 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    87541 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    87601 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    87661 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    87721 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    87781 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    87841 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    87901 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    87961 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    88021 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    88081 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    88141 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    88201 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    88261 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    88321 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    88381 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    88441 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    88501 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    88561 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    88621 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    88681 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    88741 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    88801 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    88861 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    88921 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    88981 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    89041 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    89101 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    89161 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    89221 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    89281 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    89341 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    89401 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    89461 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    89521 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    89581 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    89641 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    89701 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    89761 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    89821 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    89881 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    89941 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    90001 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    90061 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    90121 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    90181 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    90241 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    90301 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    90361 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    90421 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    90481 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    90541 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    90601 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    90661 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    90721 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    90781 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    90841 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    90901 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    90961 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    91021 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaattgat
    91081 aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt aatcaaaata
    91141 ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt ttttaagttt
    91201 gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc tgaaatatat
    91261 tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag gcgatacagt
    91321 tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc ttccagcccc
    91381 attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg caaactttta
    91441 tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa tgccaatttg
    91501 tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata gacaaataaa
    91561 ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata aatcgtgatc
    91621 tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa catgaaacgt
    91681 aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa catttttgga
    91741 tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa aatcgtaagt
    91801 gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata atagaattat
    91861 aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg ccagccacgt
    91921 gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc caattgaggt
    91981 agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg tttttttctt
    92041 tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga atttttctat
    92101 ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt gcttatttcc
    92161 cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc agacaactgt
    92221 tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt aacaattgaa
    92281 tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg ttctgaaaga
    92341 attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga caagtaagga
    92401 gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga tcctgccaga
    92461 tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct atacattttg
    92521 ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt tagaaaaaag
    92581 aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa tgacaacatg
    92641 cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg gttcccctgc
    92701 acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt atgtcgtacc
    92761 aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat tgcagccgga
    92821 aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa aaatccctta
    92881 tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa aagaaattgt
    92941 ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg aagaagcttc
    93001 cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga aaatcactca
    93061 tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt
    93121 tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta
    93181 gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat
    93241 ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat
    93301 taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta
    93361 tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc
    93421 gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac
    93481 gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc
    93541 gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta
    93601 gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat
    93661 caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact
    93721 ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat
    93781 cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact
    93841 tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct
    93901 ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa
    93961 atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt
    94021 ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt
    94081 tttcctgaac tagttatcag agagacaacg aattaagtct tattactata tatatattta
    94141 tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct
    94201 gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga
    94261 cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac
    94321 aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg
    94381 tttgaacaga tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag
    94441 cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa
    94501 ggaaaattac cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa
    94561 aaactttttg gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag
    94621 catactacac gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct
    94681 aaatatgtac ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat
    94741 ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg
    94801 ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg
    94861 ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa
    94921 cgctcattac aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt
    94981 aaataagtga tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt
    95041 ccttatttta tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag
    95101 cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca aactattttc
    95161 gcagatcgtt tgcaaaaata gtttattttt ttataaagaa gattttactg aataatatca
    95221 ttttagttta cataatgata tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag
    95281 tgctctaact gttcaagagg atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc
    95341 aattggggca aaacagatcc aatttccaga tacttcactt tattcaatgt ataagataga
    95401 atagaacagc taagtaatgt atacagaaaa tgtgtgagat ccaaataaag caaggagaca
    95461 actaaaatac caaaagtaat caagaaaaga cttttgccaa tcttaatatg aaaatatttt
    95521 tcaatggctt gataaatgat atctgttccg cctgtgcttc cgccgaatcg aaagacgaga
    95581 ccgcaaccaa tgccagaaag aatacctgtc atcaaactat tgatcagtag actatctaca
    95641 gcaaaatgta tatgaaggct ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg
    95701 acgatcgtcc ggagaaaagc gtgttttcca aaaagaaaca atgagataag caataaaggt
    95761 aaattaaaaa gcagtgaagt gacagaagga gcgaatccaa aaagattatg caaaagcaaa
    95821 gcaataccag taaccccacc atctgccagc ttagatggaa tattgattcc tgtcacagct
    95881 aatccgtaga ttcctgcgcc taaaatgatt ccgcacaatt ctgtaaataa atttaatttt
    95941 ttcatttcta gcctcttttc ttttgaaaat tttcagcaac ttctttatta tactgactta
    96001 tactcttact tcaagaggaa aatgaaaaaa aattattttt ggctgtttct ttttatttat
    96061 caaataagga gtggaaaaat gcgagcagca gacttattga caattcttac aaaatcaccc
    96121 aaaaaaacag agtatcaagg agtttttttg gctcaagaac aaaaactggt gcctgtgaca
    96181 atggtccaga taaataaagc aaatcaattt atctttttat ttgaacacgg caaagcacca
    96241 cttgctatga aagacatctt tctttttttg atgaagaacc gtgagaaagc tgtttattat
    96301 cagtcaaaag aagaactcat tcctctttat ggagtgaaag agatagaaaa taaagtggtg
    96361 atataagtaa aaataagaaa acaagacgtt tatgatgtca aaacaaccat tttttacatt
    96421 tttggcaaaa aagtgtcatg atacagtata atattcgttg aacaggccaa taaaggtgtt
    96481 ctcccccacg agatttctac tttattggct gtttcttttt attaaaataa aagttgtgtg
    96541 tgtaaaaaag atatagctga ttccaagtga caatttcgta tagattttta tggtattcgt
    96601 agtgaaaaaa cgagaaccaa aaagttgatc cgactttgtt gcttggattc tttttttgtc
    96661 agcatattta gaggtatttt tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca
    96721 agttagttgg gaaaaaggaa agaaatgttt atacttaaag aataacagaa aggaaggtcg
    96781 catgaataat tttatacaaa cattaagtga aagaaaaggc gagttgctgg aagctgcatt
    96841 tcaacatctg tccatttctc tcatttcttt gctgattgca gcactgattg ctattccttt
    96901 agcgatttgg gcagccaatc ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt
    96961 gctccaaacg attccttcac ttgccttatt aggtttactt atcccatttg tagggatcgg
    97021 aactgttcca gcattgattg cacttgttat ttacgctttg cttccgattt tccaaaacac
    97081 gtatattggt ctagctgaaa ttgacccgtc tattgaagaa gcagcggtag catttggtat
    97141 gtccagaatg agacgcttgc tgaaagtaga gcttcccatc gcattacctg tcattatctc
    97201 aggtatccga actgccttag ttttaatcat cggtacggct actttggcag cattgatcgg
    97261 agcaggtgga ttagggacat ttattttgtt aggtattgac cgaaatacac ctgttttgac
    97321 attgattggt gcgatcagtt ctgccttgct tgcaattata ttcagcggac tgattcgctg
    97381 gttgcagcat aagaaacctc gatattccat cattacttta gcagcagtgg tgtgtttatt
    97441 tggcggcgtt tctttatatc agtctgcctt gtttaaaaat gaaaccatca cgattgctgg
    97501 aaaattagga gcggaacctg aaatcctgat ccaaatgtat aaagaattga ttgaagacaa
    97561 cacaaaaaca gaagttgaat taaaaccgaa ttttggcaag acgagttttt tgttcaatgc
    97621 actggatcaa ggtcaaatcg atatctatcc tgagttcaca ggtactgtcc tagaaagctt
    97681 ggtgaaggta cctgaagcaa tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga
    97741 agcagaagaa ttattgaata agcaattcga tatgacatta ttaaaaccaa tggcttacca
    97801 aaatacgtat gccttggcag tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat
    97861 ttccgatttg gcagctatct ctgatcagat caaagcggga ttcacactgg aatttattga
    97921 tcgttcagat ggatataaag gaatccaaga agcatatggc ttgaattttt caagtgtaca
    97981 aagcatggag ccaagtttgc gttaccaagc aatcaataat ggagatgtca atattgtgga
    98041 tgcctattcc acagatagcc agctcaagga gtacgatcta gtgactctag aagatgatcg
    98101 agggttattc ccagcttatc aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca
    98161 tcctgaaatc gttaaagcac tgaataaatt aagcggaaaa ataacagagg atcaaatgat
    98221 tgatatgaat taccaagtca atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt
    98281 cctgaaaaat gaaggattag taaaggagga agatcagtga ctaaattaat cgagttcaaa
    98341 catgtgcaaa aaaagtatga tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa
    98401 aaaggagaaa tctttgtttt ggttggtcct tccggtagtg gaaaaacaac tactttgaaa
    98461 atgatcaatg gattatctaa accatcagca ggagacatct acttcaaagg aaaatcgtta
    98521 aatgaatata atctgcagaa aatgcggtgg aatatgggat atgtgctgca gcaaatcgct
    98581 ttgttcccga ccatgacagt caagcaaaat attgaagtca tccctgaaat gttaggttgg
    98641 gaaaagcaga aaagagcaga ccgtgtagat gaacttcttc aaaaagttgg cttatctcct
    98701 gatatttatc gagatcgtat gcctagagag ttgtctggag gagaacagca acggatcggg
    98761 atcatccgag ctattgctgc aagcccagat gtgattttga tggacgagcc atttagcgca
    98821 ttagatccga tttcaagaaa ctcattacaa gaattggttt tgtccttaca cgaagaattg
    98881 ggcacgacca tcgttttcgt tacgcacaat atggaagaag caatcaagct aggtgatcgt
    98941 attgcattta tgaaggatgg agaaattatc caatgtgaca caccagagca gttattgatg
    99001 aatcctaaaa acgactacgt tcggcatttc tttgatgaac cgaaacagac gaaagagtgg
    99061 cgagtagaag atttagtcgt caatggatat ttcttaaatg aaataccaga aaatgctcgt
    99121 caacaagttt gctttgatac gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca
    99181 agcattgtga tagtagaaaa gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt
    99241 gcttttttaa gtcgagagga ggaaggaaat gaaaacatat gatgcaatca tcatcggttc
    99301 aggtgtgagc ggcttaagtg ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt
    99361 ggtcgaagaa gatctatggg gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt
    99421 cttgttaagt gcagtagagg cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa
    99481 cgagatccca actgccaact gggaagaact tcaaaaattc aaacgaacat ttacggatcc
    99541 agtcccagaa agccgaaaaa aacaattagc agaagctgaa atcgatcatc tttcaggaac
    99601 cgctcgtttt ttggatgatt catctattga agtcaacgaa gaagtcttcc atgcggatta
    99661 cttagttttg gcaaccgggc aaagacctac gatcttacca gtggagggaa aggaatactt
    99721 gaaaactagt gcagactttt tatcattgcc tgtactgcct aaagaaatca tttttatcgg
    99781 tggtggttat attgcttttg agctagctac gattgccaat gctgcaggga gcaaagtaac
    99841 cattgtccat cataatcaac ggccattaaa agaatttgag gcgtctttgg tagaggaagc
    99901 cgtccatcaa atggaagcat ctggcattca gtttgctttt ggtgttgaaa cccaaaagat
    99961 catttcagaa ggtactcgct atcgtttagt cggaaaagag acggaattag tagcagatat
   100021 gattttttgt gcgactggga gacagccgaa tacagaatct ttagcacttg agcaagcgaa
   100081 tatcgtcttt gataaacacg gaattgcagt caatgattat ctgcaaacga gcaatccaaa
   100141 aatttttgcg tgtggagata ttgtttcaag gaaaacccca aaattgacac cagtcgccac
   100201 ttttgaagga aattatgtag ctaagcggat aacggatgca acaagcgaac ctatcaaata
   100261 tccgattata cctacgatcg tctacgccag tcctaaactt gctgaagtag gagttacaaa
   100321 aagtcacgcc tcttcgtcag atcaagtggt agagatggat cttaccagtt ggttcaccta
   100381 tcatcgtgtc aatgaaccag ttgctaaggc tgaattgacc tttgaccagc aaaattattt
   100441 gattggtgct gcagtgatta gcgagcaagc agacgaattg atcgatgatt tgacactcgt
   100501 catcaatcaa aaattgacta aaaaagagtt ggatagctat atcatggggt atccgacatt
   100561 ggcaagtgat ctctcctatt tattgaaata atcagcatga taactaaagc gcttgttgct
   100621 ttttccttct tgtgctacac tttaaaaaat gaatacctgt ttctggggat gtcgtagacg
   100681 acatgagagt aagttgtgaa cttagaccca ttgaacctgt cagttaatac tggtgaaggg
   100741 agaaacatag cagacgcttg aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg
   100801 ttattcatat tagaagaaag gaggtaacaa aatgaagaaa aatctttatc cagcagtcag
   100861 tatggcattc attttaatta tttggcagat tgttgtcata gtttggaaaa tacctgcctt
   100921 tattttaccc ggacctgctg ctgtattttc tgcattagcg gcagatgcac aagtattact
   100981 ttcccattct gtcacgacat taaatgaagc agtgcttggt ttggtaatag ctagcgttct
   101041 ttcttttatt actgccttgg caatggatta ttggaagttt ttagaaatgt ctttttatcc
   101101 gttactagtg atttcacaaa tgttgccaat catggtgtta gggccattac ttaccctatg
   101161 gtttggtttc ggtatgcttc caaaagtgat ccttgttgtc ttgataagct attttccgat
   101221 cgtagtttct ttttcggata cattgaggaa agtatcaaaa gagcaaatca tctttttgaa
   101281 aacgatgggg gcagatacag ggaagattta tcgaatatat aaaataccag cgggtttatc
   101341 cggcttcttt tccggtttaa aagtggctgc tacttattgt gtcagcggag cagtcgttgg
   101401 agaatggcta agcgctcaag caggcttagg gtattacatg atacgcgtaa aaaacagcta
   101461 ccagatcgat aaagtttttg ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa
   101521 tggcgcttgt tcattattga aaaaagggta ttatcgtatg ctttatattt tttaagagaa
   101581 ggggatcaaa tgaaaaaatt atggctttta ttaccgctac tgttgctttt atccgcttgt
   101641 ggcactgcaa aagaaacaag tagcaaacaa gaaataaagg atttgaaaaa agtgacactt
   101701 gttttggatt atgtaccgaa tactaatcat actggcatct acctagcaaa agaaaaaggc
   101761 tattataaag aggctggttt aaatgtccaa atcatcgaac caggagataa ttcgacaagt
   101821 attggtctag taggtgcgga caaagcacaa tttggtgtaa gttatcaaga agatgtcact
   101881 tatgctcacg cagatggaca gaatatacca gtcaaagcaa ttgctacagt gatcaaacat
   101941 aatacttctg gattcgccac cctatcagac agtaacatcc attcaccaaa agattttgaa
   102001 ggaaaaacat atgcaggttg gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg
   102061 gaaaaagacg gcggggactt ttcaaaactg acaatggtag gaagcaatgg agaagggcca
   102121 gaaagcttag gaaaatcctc agatatccaa tggtactttg aaggttggga tatgatcaaa
   102181 gcaaaagaag cgggtattga actaaactat atcccattga aagaattaga tgaacggtta
   102241 gattactata ctccggtaat tattacgaat gatcaactga tcaaaagtga tccagaattg
   102301 gtacagtctt ttatggatgc cacgaaaaaa ggctatcagg aagctattaa ggatccaaat
   102361 gacagtgcta aactgttgca aaaatatgca aaagaaaatg atcggacgtt tttagaagaa
   102421 tcacaagcat tcttgtcaaa aaattatacg gatgatcctg agaattgggg attgatggaa
   102481 gaaaaagttt ggcgcaatta tacagctttt atgcaggaaa acggattgat caagcaagat
   102541 gtaccttcgc aagaactatt cactaatcag tttataaaat aggagtgata gatatgtcaa
   102601 tccaaatcag ccacttgaat gtagtcattg aaaacctgaa aatattagaa gacatccatt
   102661 tcactatccc taaagatacc tttacatcga ttgtcgcgcc aagcggagcc ggtaagtcaa
   102721 cattgctgaa aactttaaca ggagtccagc ctatgacttc tggaacgatc aaagtagacg
   102781 atcaaaaagt aactggtctc catacagcat ttagttatat gccgcaagaa gatatgttac
   102841 ttccttggct gagcgtctat caaaacgtta cgttatatca aaaaatcaat catctaacaa
   102901 tcaatgaaga acaagtaagg gattatctgg atatctttgg gttaaacggc tatgaacatt
   102961 ttttgccgga acaattatct ggcgggatga agcagcgaac ggccttgtta cgaacaatca
   103021 tgaatccttc ttcttacttg cttcttgatg aaccatttgg agcgttagat gctatgacca
   103081 gagggcagat gcaagactgg atgttgaagc tgcctaaaaa agcaaagcgg actactttac
   103141 ttgtgacgca tgatatcgaa gaagcaattt atttatcaga caggattctt gtgctttccg
   103201 cccgtccggc acatgtgatt gctgagattc aagtgcctga aaaacgacgg tcacgtgaat
   103261 ggctactgca gcaatcagag ttgaagcaaa cgatttatca gttattggca ggtgaattag
   103321 atgttaagtg atgctcattg tcatttagat gggagtcaga gtttagcact ccttcagcag
   103381 gaacacacga ttttgacgat catcaattgt gattcgccag aagaatggaa ggaaaacaga
   103441 cagcttgcag caagtaagac tcaagcctta agttacggta tccatccttg gaaagcagat
   103501 agctatacgt ttgaacaagt tgagcctttt ttgaaaaaag caaggatcat cggtgaaata
   103561 gggttagata atatttggac gaatgtaccg atgactacac aaaaaaaagt atttgagcga
   103621 cagcttgctt ttgcagccat aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa
   103681 aaagagatct taaattatat ccaagcttac cctaatcact atttgatcca ttggtattct
   103741 tctttggact atcaacaaga ctatattgat ttaggctgtt atttcagcat tggtcttgat
   103801 ttgaaaaaga atccagcagt ctggcaacta gctaaagcag tcccaatcga ccacttactt
   103861 attgaaacgg atggactaca tgcagcagag tgggctttag gggggatttt ttcagaaaaa
   103921 gattatgtct cactgcttga agaacagtta aagacaatcg ctgagatcaa agagctatca
   103981 gttgaagaag ttaaaaaaaa tacttatcaa aacttggaac gtttcattag attaggtaaa
   104041 taaaaaagag atgtgacaca actagtgtcg cacctctttt tggctttctt tgtataaaag
   104101 taaactgttt gattaataaa tgaatcttag taccaaccgt ttgcttccca gaatgctttc
   104161 gccgcttccc aagaaccata acgtgaagta acatattgtt ctgctactct ttcttggttt
   104221 gcagcagagt agtcaccatt taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt
   104281 ccgttcgttg ctgtgtaaga accgcttgat tctttttgtg cgatccattc ttttgcagag
   104341 cttgtgcttg ctggagtagc agcagtttct gttacagcag gagcaggttg tgtttcagca
   104401 acaggcgctg cttgtttttc cgtttgaacc gtttcttgta caacaggttg ttcagcttgg
   104461 actggtgctt cttgaacggc tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt
   104521 gggattgtta attgttgtcc tgagta
//
LOCUS       DACDNO010000002        89193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDNO010000002 DACDNO010000000
VERSION     DACDNO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.002"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(282..479)
                     /locus_tag="IWI22_000097"
     CDS             complement(282..479)
                     /locus_tag="IWI22_000097"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167524.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(625..1503)
                     /locus_tag="IWI22_000098"
     CDS             complement(625..1503)
                     /locus_tag="IWI22_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9167525.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1542..2660)
                     /locus_tag="IWI22_000099"
     CDS             complement(1542..2660)
                     /locus_tag="IWI22_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9167526.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2657..4756)
                     /locus_tag="IWI22_000100"
     CDS             complement(2657..4756)
                     /locus_tag="IWI22_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9167527.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4807..5628)
                     /locus_tag="IWI22_000101"
     CDS             complement(4807..5628)
                     /locus_tag="IWI22_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9167528.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5625..6434)
                     /locus_tag="IWI22_000102"
     CDS             complement(5625..6434)
                     /locus_tag="IWI22_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9167529.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6455..6949)
                     /locus_tag="IWI22_000103"
     CDS             complement(6455..6949)
                     /locus_tag="IWI22_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9167530.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6961..7449)
                     /locus_tag="IWI22_000104"
     CDS             complement(6961..7449)
                     /locus_tag="IWI22_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9167531.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7526..10222)
                     /locus_tag="IWI22_000105"
     CDS             complement(7526..10222)
                     /locus_tag="IWI22_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9167532.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10650..11723)
                     /locus_tag="IWI22_000106"
     CDS             complement(10650..11723)
                     /locus_tag="IWI22_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9167533.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11720..12547)
                     /locus_tag="IWI22_000107"
     CDS             complement(11720..12547)
                     /locus_tag="IWI22_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167534.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12548..13354)
                     /locus_tag="IWI22_000108"
     CDS             complement(12548..13354)
                     /locus_tag="IWI22_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167535.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13366..14451)
                     /locus_tag="IWI22_000109"
     CDS             complement(13366..14451)
                     /locus_tag="IWI22_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167536.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14468..15010)
                     /locus_tag="IWI22_000110"
     CDS             complement(14468..15010)
                     /locus_tag="IWI22_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9167537.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15268..16257
                     /locus_tag="IWI22_000111"
     CDS             15268..16257
                     /locus_tag="IWI22_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9167538.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16254..16991
                     /locus_tag="IWI22_000112"
     CDS             16254..16991
                     /locus_tag="IWI22_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9167539.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNCLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEETQSKSEIAALITEKIRLEESK"
     gene            16988..17539
                     /gene="coaC"
                     /locus_tag="IWI22_000113"
     CDS             16988..17539
                     /gene="coaC"
                     /locus_tag="IWI22_000113"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9167540.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17555..18187
                     /locus_tag="IWI22_000114"
     CDS             17555..18187
                     /locus_tag="IWI22_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9167541.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18272..18919
                     /locus_tag="IWI22_000115"
     CDS             18272..18919
                     /locus_tag="IWI22_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9167542.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18924..19907
                     /locus_tag="IWI22_000116"
     CDS             18924..19907
                     /locus_tag="IWI22_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9167543.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMKANKNIGKIILKITE"
     gene            complement(20310..21824)
                     /locus_tag="IWI22_000117"
     CDS             complement(20310..21824)
                     /locus_tag="IWI22_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9167544.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21962..22768
                     /locus_tag="IWI22_000118"
     CDS             21962..22768
                     /locus_tag="IWI22_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167545.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(22943..23512)
                     /locus_tag="IWI22_000119"
     CDS             complement(22943..23512)
                     /locus_tag="IWI22_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9167546.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(23595..25657)
                     /locus_tag="IWI22_000120"
                     /pseudo
     CDS             complement(23595..25657)
                     /locus_tag="IWI22_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25934..27412)
                     /locus_tag="IWI22_000121"
     CDS             complement(25934..27412)
                     /locus_tag="IWI22_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9167547.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(27710..28645)
                     /gene="murB"
                     /locus_tag="IWI22_000122"
     CDS             complement(27710..28645)
                     /gene="murB"
                     /locus_tag="IWI22_000122"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9167548.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(28718..29638)
                     /locus_tag="IWI22_000123"
     CDS             complement(28718..29638)
                     /locus_tag="IWI22_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9167549.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            29751..30503
                     /gene="xth"
                     /locus_tag="IWI22_000124"
     CDS             29751..30503
                     /gene="xth"
                     /locus_tag="IWI22_000124"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9167550.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            31489..32022
                     /locus_tag="IWI22_000125"
     CDS             31489..32022
                     /locus_tag="IWI22_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9167551.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(32512..33036)
                     /locus_tag="IWI22_000126"
     CDS             complement(32512..33036)
                     /locus_tag="IWI22_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9167552.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(33029..33502)
                     /gene="tsaE"
                     /locus_tag="IWI22_000127"
     CDS             complement(33029..33502)
                     /gene="tsaE"
                     /locus_tag="IWI22_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9167553.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(33625..34605)
                     /gene="pta"
                     /locus_tag="IWI22_000128"
     CDS             complement(33625..34605)
                     /gene="pta"
                     /locus_tag="IWI22_000128"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9167554.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(34758..35438)
                     /locus_tag="IWI22_000129"
     CDS             complement(34758..35438)
                     /locus_tag="IWI22_000129"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9167555.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            35564..36433
                     /locus_tag="IWI22_000130"
     CDS             35564..36433
                     /locus_tag="IWI22_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9167556.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            36459..36974
                     /locus_tag="IWI22_000131"
     CDS             36459..36974
                     /locus_tag="IWI22_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167557.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(37021..37584)
                     /locus_tag="IWI22_000132"
     CDS             complement(37021..37584)
                     /locus_tag="IWI22_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9167558.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(37639..37818)
                     /locus_tag="IWI22_000133"
     CDS             complement(37639..37818)
                     /locus_tag="IWI22_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167559.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(37895..38404)
                     /locus_tag="IWI22_000134"
     CDS             complement(37895..38404)
                     /locus_tag="IWI22_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167560.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(38500..38880)
                     /locus_tag="IWI22_000135"
     CDS             complement(38500..38880)
                     /locus_tag="IWI22_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9167561.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            39210..39959
                     /locus_tag="IWI22_000136"
     CDS             39210..39959
                     /locus_tag="IWI22_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9167562.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(40143..41420)
                     /locus_tag="IWI22_000137"
     CDS             complement(40143..41420)
                     /locus_tag="IWI22_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167563.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(41499..42143)
                     /gene="pcp"
                     /locus_tag="IWI22_000138"
     CDS             complement(41499..42143)
                     /gene="pcp"
                     /locus_tag="IWI22_000138"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9167564.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(42342..43286)
                     /locus_tag="IWI22_000139"
     CDS             complement(42342..43286)
                     /locus_tag="IWI22_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9167565.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(43288..43974)
                     /locus_tag="IWI22_000140"
     CDS             complement(43288..43974)
                     /locus_tag="IWI22_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9167566.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            44448..44768
                     /locus_tag="IWI22_000141"
     CDS             44448..44768
                     /locus_tag="IWI22_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9167567.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            44922..45827
                     /locus_tag="IWI22_000142"
     CDS             44922..45827
                     /locus_tag="IWI22_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9167568.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            45824..46597
                     /locus_tag="IWI22_000143"
     CDS             45824..46597
                     /locus_tag="IWI22_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9167569.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(46778..48202)
                     /locus_tag="IWI22_000144"
     CDS             complement(46778..48202)
                     /locus_tag="IWI22_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9167570.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            48413..48859
                     /gene="nrdI"
                     /locus_tag="IWI22_000145"
     CDS             48413..48859
                     /gene="nrdI"
                     /locus_tag="IWI22_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9167571.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(48924..50906)
                     /locus_tag="IWI22_000146"
     CDS             complement(48924..50906)
                     /locus_tag="IWI22_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9167572.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(50920..51816)
                     /gene="murQ"
                     /locus_tag="IWI22_000147"
     CDS             complement(50920..51816)
                     /gene="murQ"
                     /locus_tag="IWI22_000147"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9167573.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(51819..52880)
                     /locus_tag="IWI22_000148"
     CDS             complement(51819..52880)
                     /locus_tag="IWI22_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9167574.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(53091..53618)
                     /locus_tag="IWI22_000149"
     CDS             complement(53091..53618)
                     /locus_tag="IWI22_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9167575.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(53685..55187)
                     /locus_tag="IWI22_000150"
     CDS             complement(53685..55187)
                     /locus_tag="IWI22_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9167576.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(55445..56566)
                     /locus_tag="IWI22_000151"
     CDS             complement(55445..56566)
                     /locus_tag="IWI22_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9167577.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            56692..57633
                     /locus_tag="IWI22_000152"
     CDS             56692..57633
                     /locus_tag="IWI22_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9167578.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(57783..58679)
                     /locus_tag="IWI22_000153"
     CDS             complement(57783..58679)
                     /locus_tag="IWI22_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9167579.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            58948..60288
                     /locus_tag="IWI22_000154"
     CDS             58948..60288
                     /locus_tag="IWI22_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9167580.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            60506..60949
                     /locus_tag="IWI22_000155"
     CDS             60506..60949
                     /locus_tag="IWI22_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9167581.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(61453..62871)
                     /gene="nhaC"
                     /locus_tag="IWI22_000156"
     CDS             complement(61453..62871)
                     /gene="nhaC"
                     /locus_tag="IWI22_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9167582.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(63274..64497)
                     /locus_tag="IWI22_000157"
     CDS             complement(63274..64497)
                     /locus_tag="IWI22_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9167583.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(64528..65367)
                     /locus_tag="IWI22_000158"
     CDS             complement(64528..65367)
                     /locus_tag="IWI22_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9167584.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(65384..66064)
                     /locus_tag="IWI22_000159"
     CDS             complement(65384..66064)
                     /locus_tag="IWI22_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167585.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(66066..67139)
                     /locus_tag="IWI22_000160"
     CDS             complement(66066..67139)
                     /locus_tag="IWI22_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9167586.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(67786..68457)
                     /locus_tag="IWI22_000161"
     CDS             complement(67786..68457)
                     /locus_tag="IWI22_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9167587.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(68544..71222)
                     /gene="mgtA"
                     /locus_tag="IWI22_000162"
     CDS             complement(68544..71222)
                     /gene="mgtA"
                     /locus_tag="IWI22_000162"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9167588.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            71881..72318
                     /gene="ndk"
                     /locus_tag="IWI22_000163"
     CDS             71881..72318
                     /gene="ndk"
                     /locus_tag="IWI22_000163"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9167589.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(72340..73206)
                     /locus_tag="IWI22_000164"
     CDS             complement(72340..73206)
                     /locus_tag="IWI22_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9167590.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(73366..75459)
                     /locus_tag="IWI22_000165"
     CDS             complement(73366..75459)
                     /locus_tag="IWI22_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9167591.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(76208..76831)
                     /locus_tag="IWI22_000166"
     CDS             complement(76208..76831)
                     /locus_tag="IWI22_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167592.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(76990..78387)
                     /locus_tag="IWI22_000167"
     CDS             complement(76990..78387)
                     /locus_tag="IWI22_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9167593.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(78419..79390)
                     /locus_tag="IWI22_000168"
     CDS             complement(78419..79390)
                     /locus_tag="IWI22_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9167594.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            79764..80189
                     /locus_tag="IWI22_000169"
     CDS             79764..80189
                     /locus_tag="IWI22_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167595.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(80677..81378)
                     /locus_tag="IWI22_000170"
     CDS             complement(80677..81378)
                     /locus_tag="IWI22_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9167596.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(81394..81834)
                     /locus_tag="IWI22_000171"
     CDS             complement(81394..81834)
                     /locus_tag="IWI22_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9167597.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(81834..82409)
                     /locus_tag="IWI22_000172"
     CDS             complement(81834..82409)
                     /locus_tag="IWI22_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9167598.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(83257..83736)
                     /locus_tag="IWI22_000173"
                     /pseudo
     CDS             complement(83257..83736)
                     /locus_tag="IWI22_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(83895..84596)
                     /locus_tag="IWI22_000174"
     CDS             complement(83895..84596)
                     /locus_tag="IWI22_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9167599.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(84704..86113)
                     /locus_tag="IWI22_000175"
     CDS             complement(84704..86113)
                     /locus_tag="IWI22_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9167600.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(86161..88026)
                     /locus_tag="IWI22_000176"
     CDS             complement(86161..88026)
                     /locus_tag="IWI22_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9167601.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(88147..88998)
                     /locus_tag="IWI22_000177"
     CDS             complement(88147..88998)
                     /locus_tag="IWI22_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9167602.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT        26091 a        18653 c        15441 g        29008 t
ORIGIN      
        1 taaaataaaa aatttattgc aaaaaaaata gaagtgacaa aattatcaat catttcaagg
       61 taatataact acctgagttt attatcgatt aaaatgctaa ctacatttgc aagagaagcg
      121 agagttaaag gtaaatatag aatttctact tttttagcaa tagaaatagg aataaaatat
      181 ttaaaatgta tagaaacttt cttttttttc ttgctatctt tttataatca caactctcta
      241 taatagaatt acgatttatt ttatacggct gaaaaaatct atcaccaaaa aaattcaaac
      301 attaagccaa ggattatgaa gtcaacattt cgaaaaatga acatacattg gtaagcaact
      361 ataactatta ccacagacat aaagtaacac aatatattac tatttaagtg tacgccacca
      421 gtgaatatcc tcaaggtata cagcgcacaa caaaaagaaa aaaggaaacg cagaagcatt
      481 tcctttcagt gatttaatga aaacaacacg atttgactta gagccttttt atttgaattc
      541 attttcgctt tatccaactt catttgatca tttgattact cggttaaata cttttgacat
      601 gagtatcaat gaaacattca ttaattatgg ttgcttattt cttaccgctt ttctggccaa
      661 tgctaatccg ccgattattc cggcattatc acctaattcg ggcgtaataa tgtagctatc
      721 aattggtggt atttcaacat atccatttat aagctcaaca aatttatcct gaactttttt
      781 cttcatgtgt tcttgtttca ttacaccacc gccaaaaata attctatctg gcgaaaacat
      841 cagtgttgta ttgtatgcac attgggcaag gtaaaaggct tcaatctgcc aaaacgaatc
      901 gtcagccaat aaattctggc cttttacacc caaccgtttt tcaatcgctg gtcctgctgc
      961 catcccctct aaacaatctt ggtggaaggg acagtttcct gaaaaattgt cctttggatg
     1021 tcgcttgatc aacatatgcc ccatttccgg atgactgaac ccttccacga aagtcccatt
     1081 gaacaaggca ccacctccga tacctgttcc cacagtgaag tatgcaacac ctgctaaccc
     1141 tttgccagaa ccagcaacat attcaccata gcaagccgca ttgacatctg ttgtccattc
     1201 aatcggaata ttgaaatatt gctttagcgt tcctacaaaa ttgaaatttt gccaagcaag
     1261 ttttggtgtt gatgtgatat gaccgaaact cgttgatttg cattgaatat caattggacc
     1321 aaagcagccg accccgatgc ctgccaattg ttccttaaaa ggtttaaaaa aatcaatgac
     1381 ctgcttcatt gtttcaactg gagttgttgt gggaaaactg actctttcca aaatcgttaa
     1441 ttcctcagta ccgactgcac agacaaattt agtccctcca gcttcaattg ccccatatat
     1501 catcataggt tcctcccttc gaatatgtga aaaacacttc attaacgcgg caagcgttct
     1561 cgatcagtga aatgacgttt agtatttcct gaagcttctg ttgaatacca gtaagcgaca
     1621 ctagcaacat cgtcttgccg ctcaaataag cgaacatcat cgttgccgat ttgttgtaaa
     1681 gtgaccgata aatcttcggt aaaacagatg gggtcttgaa gatgccaacg ataaagacca
     1741 tgtcgtggca ttgcgtcgtc accaaaggca tgaacggcat tcaattttcc cgtttcaaaa
     1801 cgatctcttg tggcatctct atttgattgg taaggatacc ctaaaaaggc tgttgaatag
     1861 tttttcgcat gtggacgtcc gaactcatcg tactcatgaa aggcccacgc cccgccaaaa
     1921 taatcttctg aacctgttga ggttacggtc ggatactcac tatccccatc aatgtagaat
     1981 ttaaactcgc cttcgcccca ccaataacgt tctaggcagg taagcgcaag gtaagtaccc
     2041 acgtaatagc cttgaccctt taatcgatca attaacttat aatcctcctg taatttggta
     2101 atgcgttccc gatgccaatt tgcgtgaaaa tataggctat tttcttccaa ttcgtccacc
     2161 aaagcgtaat ttatcgtata aaaaaatgag gacatatcct tcggatgtaa attctcgatc
     2221 gtaattttag cggatgtttg gaaaggcatt tcgaaatagg aattaaagcc ccctgtagga
     2281 ttaaccacta tcggcaacga gttcacctcg caacgctctc caaacccatt acaaaagaaa
     2341 tcccctaaag gactttcaac agcagggcta tccgacccat cccaataaat tcttaagatt
     2401 aaatcacgta aaacaaaact gccaaattct gttcgatcac gaatcgtcat ccacatatga
     2461 cgaattattc ccgttccagt aatatccgca atcacagttt cttcaccaga agtcagcgga
     2521 attgcgccac gacctttgcg gcttggtcct agctcgcttg gcaacatcgc agctttacct
     2581 ttctcgcctg ttggattttc gggagtgatt gctcgactct ggattttttt aaattgggta
     2641 atatttttta gtatactcat ctttttctcc tttgaatgtg aatcgttttt cccttttcta
     2701 cgtgaagaat ttttttcgta tcacttattt tcaatggctt ttctgacaaa tttgaaatac
     2761 tcaactcgga ttgcgtacat tcaatcgaaa tgaaggcttg accgaacttc aaattcttcc
     2821 aagaaaacgt taaattatca acaggatcaa tttgcaatac tccttttttg aatttcaatc
     2881 cgaaatattg actgatcatt aggctgacaa aaagacccga tgcccagccg catttgccgc
     2941 agccaaagtc tcgcacgact tctccttgtt gagcctctaa gcgatacggc caccaccacc
     3001 acgaaccgtc taaatctgcc aaacaaatta gttcttctaa tcgggttacg aaagcttgag
     3061 ggttgtgaag ctcgctcatt aaaacagtga taaagccagg aaaagtggcg ccggattcta
     3121 atccccaagt gatgcctttg atttcctcgc tataggttgg attgaaagaa ctagcagaaa
     3181 aggccatggt gttctgatat aaatgatctt tattatctaa aaattgataa aaaggcatca
     3241 acgtagtgtc tgattcttct ccatcatgca tcaataaatt aatttttcca tccacaatca
     3301 catcacttaa aaagattaac ccttgctcaa gaatcggttt ttgatagtgc ttcactgaat
     3361 aggttgtttt ctcctcatca ccgattccct ccaaaaattg ctcaccaaaa ggaccatcga
     3421 tagtcatttg tttacaaatt gattccttta gtttacaggc aatgtttttg tatcgcttgc
     3481 taagactcag ttgttctagg gcttcaaaaa tttctgcaag tccaatacag gccttccaaa
     3541 gacaaagatt gctgccggta tgatactttc caagtgcaaa ggcgtctgat aaccaaacag
     3601 atggaaaaag cattggttcc cctgaccgac gttgagctaa tatgtcctca ataattcttg
     3661 aagcattcag tagaacctgc ggacaccgct caaagaattt tgtatcccca gtatgttcaa
     3721 aataaagact agccagtaac gcactggata acgaattact cagagaatga ttgatacctc
     3781 ctgtaaattt tgaaccagga aacttaacgc catattgatc aaaccataga accgtttttt
     3841 gacataactc cggttcaaac atggtgaatg gcaatgatgc gtaatacata tccttattcc
     3901 aaattcgatt agtagcagga tagctgcccc aattgctgcc gacgacctgt ccctgtcgat
     3961 tcataccgaa actagaaaag ctttgataaa gtgcacgacg atagagatgg acaactctat
     4021 cgtttggcat tgacagctgc ccaaatagct gagcaaaata gttcaatgtt tccttcagcc
     4081 atttttcgat atcagattcc tgaaaagtca ttacttcttc aaaagcattt gggtccacaa
     4141 aagcaatggc gaagcttgcc acttcctgtt gtgcgatcgt gtccttattt cctttttgag
     4201 aaatcaacac attttgctga tccgaatact tctcttgata cgaaggcaca tctttcactg
     4261 aaacttgaag gctttcgtgt gtttcattca caatgtaaac ctgttgaaca agcatactta
     4321 aacgcttatt agccagtatg ggagcaaagg atactacaat gaccttaaac gtcgaataat
     4381 gatgaatata tctaggcaaa cagtcttgaa tcacatcgac ttcaatacga tgatccgatt
     4441 ctgacaaatg gtagacaatc ccgtttactt caacctttaa ggataacgaa ttggtttgag
     4501 agagctgttt attgacccaa acacctggtt tttcctcagt tagtaaattt tctcgataaa
     4561 aggtcaacgt ttgaatcgtc ccgcaaacat caaggtttgc taatgtcttt tgattactta
     4621 catcgaaggt caatcccttt acatcaggaa tattccgata gtagctgtat tcgtttgaag
     4681 tagtcacatt tccgtcgttt aactcagcat taaaaaatgg gtgtttgggt gaataaatta
     4741 cttcattaat agtcatcttt tccctccttg ctaaaaaaac tgcagcctag tagctgcagc
     4801 ttcctactat gcgattccaa gtaatgacaa taaaattccc aagacgacca ttccaaaaat
     4861 aattttgatg acgccgactt ttttatctag taatttgaaa cagctcaatg ttaccaataa
     4921 cggtacaatt cctttaaaaa tactatcgag gatttcttgg acattcatta gttcttcgcc
     4981 cttcattgaa aagctcaaaa cggtattgaa tttgaccata ctgatcgtca ttgcacctac
     5041 catgatcaga cccaagatac tagcagcttt tgtcagaatt tcaattaacc cgttcgcata
     5101 cagcttttca atatatttag agcctaatga atagcctaaa acgccgccat agtatcgcat
     5161 caagtaatta ggtatattga acataactaa aaagataatt ggtgccaaaa tatttccggc
     5221 attggctaag ttgatcgcta gtcccgcggt aacgacacgc caaactcccc agaacaatga
     5281 atcgccaatt ccagccaatg gtcccatcaa actcgttttg atcgcgttga tactttccac
     5341 gtcaaaacca gctttttcag aattttcctt ctccatcgat gccgcaatcc ccatgacaaa
     5401 ggacgcgcaa ggaatggtcg tcgaaaaata ctgtgtatga cgttccagcg ccgcagctcg
     5461 atcttcttca cttttgtaga aatgattaat gaagggcatc atagaatagg cgaaaccacc
     5521 gccgcccatc gtcaccggat tcaccgaagc atacatagtc atcgagcgcc aaaacatctt
     5581 ccgaaccaat tttttctcat ctttagaaat tccatcagct aatttcattg aaagaaatcc
     5641 tcctcttctt ggtcaagcgt tgcatttaca gcagcatcgc tttgaactgc atgaacagat
     5701 atatttttta gttgtagatc tctttgggct ataacaattg caataatcgt tccaactgcg
     5761 gcaattgcaa tcaatggtaa ctcgagatac gccataacaa cggccccaaa gaaaaaccac
     5821 acagcaatat ttttactcca aagcatccgt aacagcatcg ccatcccaac tgccggcagc
     5881 agatttccag cgactgttaa tccgttcatg atgacctctg gaatattatc caagacgctt
     5941 ttgacaacat ctgctccaaa taaaacggca aagaatggaa taagagcata aagcgcatag
     6001 tggacaaacc acataaagaa gtgcagattg atgattcctt tttgatcacc agattttgca
     6061 aatttgaaaa atttagcagc catcggtcca catacaaaag aaaaaatcat attcattaac
     6121 tgtaatccta atactgctag tggaaagaca atgggaataa tcgcatccac gtttcctccc
     6181 attttgattg cgaacgctgt agccagccca gcagccaaag aaggctccgc cgtactagag
     6241 attccgatat tgacgacacc cataaagatg gcttctagtg acgccccgac aatcaacccc
     6301 gtctgcaaat cccccatcaa cgctcctgct aacggagcaa caacaatcgg acgactcagc
     6361 atggacatcc cgaacatttc atgtccagca actccaataa aaacggccaa agcgactaca
     6421 aatgcatcat acataatttt tctccttcct ttttctaaaa atcactcaaa atagagatag
     6481 ctgaacgttt ttccatcgcc ggtgtaggct gaaaaacggt atctggataa agttcaacta
     6541 attcttttaa gtcttgaatt tctgacgtcg ttaacatcac ttcctttgat aaggttttct
     6601 tatcttccga tggcggtgtc attttcccag cattccccac gttcattaat tcgatttccg
     6661 ttgggaaagc tttcgcaact tttaccgcat ctttgacatt agggactaaa accatcaagc
     6721 gcatgtcctt cgcccgcgga tcttttaaca aatgaatcgc gtcatcaact gttttaataa
     6781 tgactttgac attcgatggt gcagccatct tcaatgtcat cttttgaata tcgttggtag
     6841 ccgcctcatc gcttgctact actaacccgg ttaaatttag actctttgtc caagtcatcg
     6901 ctacttgtcc atgaattaat cgttcatcaa ttcgcactgc tttaatcatt tttcttcctc
     6961 ctaaaaaaaa tcttcctctt catttactac atgtacctca tcaagaatgg tcatctttaa
     7021 ttcctgttgg catttattta ccagttcctg aatatcctcc tttgttaatt ccttggttgc
     7081 caatactatt tcgagcagaa ttggcagatt aaagccagac aaaatagtta cagcaatctc
     7141 tttctccaaa aaggttttgg taatcttctg attgacactt ccgccaaata aatcggtaaa
     7201 aacaaaaatc tgatcctgct cgttcacctg gtttacaaat acattcaact cctgttcaaa
     7261 atcacactca tccacataag cattaatcac cgtaatctct tgaaccttcc ccgtcagtaa
     7321 ttgaatactg ctaagaaagc ctgaagctaa gtgaccgtga gtcgctatta gaattttctt
     7381 catatggtta attcctttct ttctgttcac ttttatataa gcaattcctg tgccaacttt
     7441 taaaaacatt ggtttaatca cgttaattca cattaaaaaa agtgtctaac ctaaaaaaaa
     7501 cagattagac acttttttag acactttatt tttgaatata cggtcctaat aattcataga
     7561 gcagcgaaag ctcataagca ttgactttaa aattatattt catctccatt gattgaaaaa
     7621 tacttctaga aacataataa aattcatctt catctggttt caaatcataa ttcggttctt
     7681 cagatttttc tgtccgagcc aagaataaac gttccatcat caatgccgta tgcatataga
     7741 gatttagttt tacacggcca tttaaagtga aatgatagta attttcatat ttagaaatga
     7801 ctgtttccac ttcactcatc acgatcgttg ggttcagaaa gttcaaacgg tccgcaattc
     7861 cttccaacga aaagaatttt actagctcct gaatcaagcg gttaaattct gccggcttca
     7921 tttcaggact caaaatatgc tccaaattgg ctgctccatc accgtctaag atgtcataaa
     7981 cattaatatt aggaattgaa aaggaacgtg gaagatccga agtcgtgatg accaattttg
     8041 tcttgtcaaa ataagtttca tcattttggt cgattaattc acgtagttct ctgtagtcct
     8101 tcgtgaaaac ttcgatggat tcatccgata aatgatgacg aaaaacatct ttcaatttgt
     8161 ccgaaatccc caaacccgac atacaggaaa tgataatgtt gctactttga gaaaagcctt
     8221 caaagtattg aacattgatt gaatacttgt ctgctgcatc ctccgcaatc tgtttaaatg
     8281 gcgcattcat cgtcatcttc aagccaatat caagtgcaat ggaagtcgtc aaattattca
     8341 gaagaagtaa ctcccctttt aaatcgttct tgatctgtgt gtaaatttga tttaaagagc
     8401 ccatatctac gatcagcact agactatctg tattcacttg ccgtttcacg aaattccttg
     8461 tcgattcgac aatctctgac acattactct ctactggcat atccaacgct tcaaaaacat
     8521 aattgccgca taattgattc actactgctt gaatgctgct agccgtacta tctccgtgag
     8581 caagcaacag gcccttcagc tcgatggatt catctacata atcagataaa gtcacgacaa
     8641 taagcagtct tagaaagttc tcgtcctttt ctgagagcgg tggtaaatgc tccaagaact
     8701 gattacataa gtaagtcgtc cgaggtagat ttgcagctaa tgtacgattt atcccggtta
     8761 agtctgtctt atagcttggg gaaatgacat aggatctgct aataatttct gcaacaggtc
     8821 cactcgtcgt caatccgtac gtttttccta aaacggatac ccagaccttt ctaaacatct
     8881 gaatcatcaa ttttctactc attgataaaa aagaatcctt atcaatgtga tgcaccaaat
     8941 gctgtacttc attctttatc gaagaaaaat tttcattgaa attaccttca tttatcagct
     9001 gagtaatttt ttccaaaatc gaatcataat caaacggttt gattcgatcc gtttttatct
     9061 tttgattttt ctttatcaat aaactagcaa tcggcaagct ttctacttcg gattccagca
     9121 tatttaactg aatcaagatc tcatttttcc caatcgttaa tggttcttct tgtgttcgac
     9181 tatacacatc cgcacaactg attttaatga tattcttcag cttaccaata ttcccactaa
     9241 agggatgaga taacattaaa gacaaggctt ctcttgaaac aataatatct ttttgtaaaa
     9301 catccgcttc attttgaaaa aataattgaa tcagctctaa tcgttctcga atggggcgat
     9361 cttcaaaagt tggcaacata acactaattt gaatacgccg atcaaaggtg tctaataaat
     9421 aattttcacc tttttccgtc gtggcgaaaa taaaacgaac cttagcagaa tgccaattta
     9481 cattttcacc gactggacga tagtttccag tatcaataaa tagaaagagc ttttcttgat
     9541 tttctttcga taggcgatgg acctcatcga gaaaaagata gccgttgttg gccgttgcca
     9601 ataaaccatc ggtatccttt tcagcccccg taaaggcccc cttcttgtat ccaaacagtg
     9661 ttgccgaaag cagttcggga ttgtccgcat aatcggcaca gtttaagata gcaaatggtg
     9721 catctttttc aataatttgc tcatggatag cgtattcaaa aatttttgtg gctaaaaaac
     9781 tttttccaac acccgtggct ccatttatta atacactgag tccgttaggc ggatatttga
     9841 cagcggctat acatttttga atgacctctc tcagacttcc gtcatgacca actacagaat
     9901 gaaaggcttc gtttatttgc tccgtattat ctacaaccgc atattcatct aaaggcttcc
     9961 acaaaacagg ctttcccggt aatttctcga cctttttatc ttccactaat ctcgtcaaat
    10021 aatggctggc attctgacga gagataccta gattcttcga aattttctcg gcagtgacag
    10081 aagacatatt ccctgttagt cgtatcagct catcaaaaac tcgatcttta gcgctctttt
    10141 tcattttttc ctccagttta atgtgtctaa aagcataata aactatcggc tgcaatatgt
    10201 ctattctata tttgtattcc aatttttact ttgaaacaca aatagtcgaa tatttgcctc
    10261 agatatctga caaaataatt tatctaccaa tttagttttt atctaatatg atttttatgt
    10321 tttttcatag ctacaaatat tgaactggat tcatatatta gttttattaa tagattacag
    10381 aagggttaag tatatttgca tagtttccaa ttactttatc cttctgtaac tttttcaatc
    10441 ttagatttac tagcccgtct cgggccctat tttacaatgg ttttgtccac ttttctctgg
    10501 tagaaaaacg tggtagaaaa aataaaataa cacaaaaaaa gcggcgtttc atccgctttt
    10561 ggtagaagat tggaaaactc agatagaaaa aggacgattt ttagctcttt agaacgttga
    10621 taagtcccca ttcttactga gttttgccat tatctcctat acatcttgaa ttctaagaat
    10681 aggtcattat agatgcctag gtatttcgat cctagatctt tgtagacttc aagatgggag
    10741 atcgtgtcat cagatggata gaattgttcg tccgacgaaa tgtcttttgg caataacgct
    10801 tttgcttctt catttggtgt agaataacca atatactctg cattttgtgc cgcattttcc
    10861 ggttctagca taaagttgat gaaatcatag gcaccttctt tatttttcac ggtttttggc
    10921 ataacgatat tatcaaacca aagattggat ccttctggtg ggatcacata atgtaaatgt
    10981 tcattttccc acatcatatc ggcagcttcg ccagaaaatg tcactgccac ggaggcttct
    11041 tcattgatca tatacatttt gatctcatct gccacgattg ctttgacatt tggtgttaaa
    11101 tgattcaatt tatttgctgc ttctgtcagc tgtgccatat tcttactatt caaagaatag
    11161 cctaaactat tcaaagaaag ccccataact tctcgtgcac cgtcaatcag catcaaactg
    11221 tcttttaatt ccggcttcca caaatcatcc caatgcttaa tttgatttgc atcaaagaat
    11281 ttatcgttat atacgatccc taaagttccc caaaaatatg gaatcgaata ctgattatct
    11341 ggatcaaaat ccaagtctaa aaatcgtgga tcaatattat ttaatccttt gatcttcgaa
    11401 tgatctaatg gaagtactag tttttctttg atcatttttt ggatcatata ttcacttggt
    11461 atagcgatgt cgtagtttgt cccaccttgc tggatttttg tgaacatcgc ttcattagaa
    11521 tcaaatgttt cataattcac tttgtatcca tattcttttt caaatttcgt gatcaaacta
    11581 ggatcgatat aatctcccca gttatagatg gtaagtacct ttgctccact catgccactt
    11641 gttttttcaa gttgacgaac accaagcagc agcaccgcaa taatgatgag aatacctaaa
    11701 aacagagatt gtaatttttt catcgcagcc gtgccacccc ctcgcgtact cttctgtttt
    11761 tcttcagccg ttttggacgg ttttctttac taataaagta gtatccaatg accaatatca
    11821 tggagaacaa gaaaactaac gcacttagcg cattgatttc taaactgatt ccttgtcttg
    11881 cacgagaata gatctctact gagagcgtcg taaagccatt tcctgtgacg aaaaatgtaa
    11941 ccgcaaaatc atcaagagaa taggtaaatg ccataaaata tccagctatg attcctggag
    12001 aaagataagg caaaatgata tttttgacta cttggaaatt attagcacca aggtctctag
    12061 ccgcatcgac cattgaatcg ttcatttcct gcaattttgg caagaccatc aagacgacga
    12121 ttggaatgct gaaagcaata tgggaaagca atacactctg aaaacctaat ccgaatccta
    12181 gtagtgtaaa gaaaatcaag aaactagcac cgataataac atcaggagag actaataaga
    12241 tgttattcat gctcaacaac gctgtccgtg cacgtcgttt tttcgtgtaa tagattccca
    12301 tagccccaaa tgtcccgatg atcgtggcga tcaatgcaga taagaaagcc agcatgaacg
    12361 tattcaatac gataatgatc aaacgtgtat cttcaaaaac agcttcataa ttttcccaag
    12421 taaatcctgt aaagctgttc atcgttcctc cttcattgaa ggaataaaaa atcagataga
    12481 agatcggtgc atataagaat aaaaagacga tccctaaata aagatacgac catttaaatt
    12541 ttttcattta tgagcgacct ccttttttct tctcgcctgt caggagcatc acgatgaaca
    12601 tagcaatgat taaaatcaca ccgattgtag aacccatccc ccagttttgt gtaaccaaga
    12661 aatgctgttc gatagccgtt cctaaagtga tcacccgatt tccgccaatc agacgagtca
    12721 gcatgaataa agaaagagaa ggaataaata ctgcttggac accacttttc acaccattta
    12781 atgataacgg aaaaatcact cgtcggaagg tctcaaagct attcgcgcct aaatctctac
    12841 tagcactgat caaagaagga ttcatctctt ccaatgcatt gaaaattggc ataatcataa
    12901 atggaatctc gatatacgtt gccacgaaca gaaaactgaa gtctgtgaat aatatctgtt
    12961 tcggcccgat cccgataaaa tcaaagaaat gattcacgct gccgtgcaca ctgaaaatcc
    13021 caataaatgc gtaagcttta agcagcagat tcacccatgt aggcaaaata atcagcatca
    13081 gccataattg cttgtgcttc agttttgtta aaaaataagc agtcgggtaa ctgatgatca
    13141 gcgtgaaaaa cgtgatcaaa aatgcatacc atacagaatt gatcgtcata gaaagcgatg
    13201 tccccgacgt aaaataagat agataattat tcagcgtgaa ttgcccattc atatcaaaaa
    13261 aagattgata aatgatcatt aatacaggcg cgatcacgaa taaaccgagc cacaaaatat
    13321 aagggacgga atagatcctg cgcattgttt tcattcttgt cccccctatt cttcgtagct
    13381 gtcaagacgt gcatcgaatt cttcttcaga ctcgttgaaa cgcatgacat ggatatcttc
    13441 tggctcaaag gccaatccta ctttgcttcc ttctttagct tttttcgttg aatggaccat
    13501 ccattcattt tgctgttcat cataacagat gatttcataa tggacaccac gaaacagctg
    13561 agtatcgacc gttacgacta gttttccttt atctggtgtc gtgatcgtca aatcctctgg
    13621 gcgtaaaaca acttcaactg gttcatttag acgcatcccg ccatcgacac attcaaattg
    13681 cttaccaaca aactcaacta gattatcttc gatcattaca ccgtccacga tattgctctc
    13741 cccaacaaaa tcagcaacga aatgattgat tggctcatca taaatatcta cgggtgtacc
    13801 actttggacg attttccctt tattcatgac aaaaatctca tcactcattg ccaacgcttc
    13861 ttcttggtca tgagtaacaa aaataaacgt gattcctaat cgttgttgca gatcccgcag
    13921 ttcatactgc atatctgtac gtagtttcaa atcaagcgcc gataacggtt catctaatag
    13981 caatactttc ggttcattta caattgctcg tgcaatcgct acacgctgtc tttgaccacc
    14041 agacatttca ctaatctccc gtttttcgta tccaggaagc tggaccatac gcaaagcatc
    14101 ttttaccttc ttttcgatat ctgatttgga cattttcttg atcttcaatc caaaagcaac
    14161 gttatcaaaa acattcatat gaggaaaaag tgcataatct tggaagactg tatttacttg
    14221 tcgcttatta gctggcaaat cattcaaccg tttcccttca aaatagatgt ccccttctgt
    14281 agcttcggtg aatccagcta taatacgtag gatcgttgtt ttaccacagc cggaaggacc
    14341 aagcagtgta taaaattttc cttgttcgat ctcaaaactt acttttttta atactgtttc
    14401 atcatcatag cgtttcacta cttgatcaaa tgaaatgatt ggtttcccca cgtttttacc
    14461 ccctatatta caaatatgat tgcgttgcaa caatcaatac ttttgttcgt tttttgagat
    14521 gattccttaa ttgatgttgt tttgtcgctt gataatagat cgactgtcct gcttttgcca
    14581 catagaccgt ctctcctaag cttaactcta attccccttc taatacaaaa ataaacgttt
    14641 cagataatga aggttcaaac gtcttatatt ctcctgcacc ttcaaaagtc agcaagacag
    14701 gctccatttc tttttcattc gaatcagcca cgagccattc caattcatac ccgttttctt
    14761 catcgaaata aaccgtatga tcttcttttg aataaaccac ttgcagattc gctgcttctt
    14821 gatgaaaaaa ttcttctgga gtcacaccta agacttcaag aatagaaaag aatgtttcca
    14881 ttgatggtga acttaaatct cgttccaact gggaaatata cccttttgac aaatctgtgc
    14941 gttcgcctag ttcttcttgt gtcaaattct tttggatgcg caaatttctt aacttctcac
    15001 caatttccat aatttcctcc gaatcgaagt tttctaatag taaacttcct gtttactacc
    15061 actcgtatta gtatacgaaa tttattgaaa aaggcaaccc tttgtaaaat tttttttgct
    15121 gaatatttct ttatcttttg gaataaattc tagttgtttg attctttatt tttcacctaa
    15181 tttttgtcta aatttacgga agaacaagaa taaaagagta ggctttatgg tatgataaaa
    15241 gaaaatttta aaaggagtgt ctcacgaatg aacgcttttc gatcttttca ggtaataaag
    15301 gaacctgttt tccatacagc aatcagtgat ctgccattta ctgagttggc tgaaaatgaa
    15361 gtcttggtaa aaattgccta ttctgatgtc aattataaag atgcattggc aatgagcgag
    15421 tctggtcagg tgatacgaac atatccgatg actccaggga ttgatttatc cggaactgtc
    15481 gtccaaagta acaatccacg tttttcaaaa gaagatcttg tacttgctac tggttttggg
    15541 cttggcgtca cccatccagg cggttatagc caatatcaaa aagtaccagg agactggtta
    15601 gttcctctac caaaaaatat gacgctgcgt caagcgatga tccttggtac agcagggttt
    15661 actgctatgt tgtgtgtgaa cgctttaatc cgtcaaggta tgacctccga taaaaaagtt
    15721 gtcgttactg gtgcttctgg cggtgtaggc agtacagcga tagccctgtt acataaacta
    15781 ggattccagt caatcattgc cttttccaga aaagaagagt ctgtcacatg gctaaaaagt
    15841 ctaggtgctt tgcaagtcgt acgacctgat gaatttctgc cagagacaac aaaagcttta
    15901 ggaaaacagc aaatcgacta tgtcatcgac acagtaggtg gcgagcagct aagcagtctt
    15961 cttccattga tctcttataa cggtgcagta gctttatgtg gcaatgctgg gggaatcaaa
    16021 ctaaacgcga ctgtcttgcc ttttattctg cgaaacatcc agttgattgg aattgattct
    16081 gtcaatgtac caatagatca acgtctttct ttatggcaac agatggcgga tttgcagatt
    16141 gcagatgaac tggtcgtcca agagattact ctggatcaat tgcctgaaac agcatctaaa
    16201 ctacttgctg gaacacacca aggaagaaca ctagtaaatg taggtgacca taaatgaaga
    16261 ttctaatcac agctggagga acgactgaaa aaatcgatca agtacgtgct attacaaatc
    16321 attcgacagg acgattaggg caagcattag ctgattattt agctgccaat ccggatacca
    16381 caatcgatta cgtgactacg aaacaagcac tgaaaccgaa aagacactcc aatataacga
    16441 tttatacgat cgaatctgca ctggacttat ttttacaatt agaagaacta acgaaaaaag
    16501 aacactacga tgcgattatc cacagtatgg cagtcagtga ttttactcct gcttttagtt
    16561 tttcagaaga acagctggca aaaaacctac ccacttcttc aacgcaagaa gaactggaca
    16621 attggtttgc ggaaaatgaa cagacagata ccactgtttc aaagatctct tctaacaccg
    16681 agcatcttgt gctcgttttg aagaaaacac caaaaatcat ttcttatcta cgtgaatggc
    16741 agccaaaagc aaaaatcatt ggtttcaaac tattagtaga tgtaccaaag gaatctcttc
    16801 tggcagtcgc aaaaaattgt ctgataaata acaaaactga ttttattttc gcaaatgatt
    16861 tgacggaaat ccatggcgag acacaccacg gctatctctt atctaaagat ggaacagtgg
    16921 aagaaactca aagtaaatct gagattgctg cattgattac tgagaaaatt agactggagg
    16981 aatcaaaatg aaaaagatct tactaggcgt tactggaagt atttctgctt acaaaagcgc
    17041 tgatcttaca agtcaattcg tcaaattagg tgcccaggta gatgtgatta tgtctaaaag
    17101 cagcacacat tttatcacgc cattaacctt gcaatctctg tcgaagcgcg cagtccatac
    17161 agacgtcatg caagaatcaa atccgcaggt aatcaatcat atcgaactag caaaacaagc
    17221 tgacttgttt ctcatcgcgc ctgctacagc aaacattatc ggcaagctag caaacggcat
    17281 tgcagacgat cttctttcaa cagtcgcaat ggcgcttcca gtgggaacac caaaattgat
    17341 tgcccctgcc atgaacacga atatgtatca aaacccgatc actcagcgta atctggaaac
    17401 attgagatct gtcggctatc aggaaattga accaagagaa tctcttttag cttgtggaga
    17461 ttttggaaaa ggtgccttag ctgatattca gacgatcatc gaacaggtca tgctgactct
    17521 taatgaaaga aatgcttaga aaaaaggagt acttatgaaa aatacaaaaa actttacatt
    17581 aactgcgatg tttttagcta ttttgatttt attagctgtt acgcctttag gttttatccc
    17641 tatcggtcca atcaatgcaa caaccatgca tattcctgta atcattgctt cgatcgtttt
    17701 aggaccaaga ctaggtgctt ttcttggcgg tacatttgga ctgatcagca tgatccgtag
    17761 cacagttatc caaacacctt tgtcttttgt cttttctccg tttatcccag tcatcggaac
    17821 agaacatgga agctggaaag ccttgctgat cgcgtttatt cctcgcatct tgattggggt
    17881 ggttccttat ttcgtttata aaggtctgcg caaactaatg aaacaaaaag cagattccgt
    17941 ttctttattc ttagctggac tttccggttc attgaccaat acgattttag tgatgaatat
    18001 gatctacttt ttattccagc aagattatgc caaagtcatc ggaacaaatt taaatgctgt
    18061 ttattcagct attctagcgg ttatttttac aagtggtata cctgaaggaa tcgtcgcagg
    18121 tcttgcgact gcagcagtat gcaatgtctt gttaagatac ttcaagcacc cttctcttac
    18181 taactgatag tcatcgtagc cgatctctga tttttttctc ttttggtgtt actattgaat
    18241 ggaaagattt gacgtcgaaa ggatgatcca aatgaaaatt tttgttgtag gtgcaaacgg
    18301 acaaattggc cgacacctga taaaagattt agctccaagt tcccatgaaa tttttgcagg
    18361 ggtgagagat gttgcttccc aaacattagt aaaaaaagag aatgtttcgt atgtctcttt
    18421 tgatttaact tggtccgtgg aaaaaatgac cgaagcattc aaaggcatcg atgtattgat
    18481 ttttgctgcc ggctctcaag gaaagaatct tctgcaagta gacttggatg gcgcaatcaa
    18541 aacagtgatc gcagctgaaa atgcgcatgt ctctcgctat ctgatggtca gtgctgttta
    18601 tgcagatgaa ccagcaaaat ggcctgagtc catgacagat tattatatta ctaaacatta
    18661 tgcagatgaa tggctgaaac gaactaactt agattttgtc attcttcaac cagtcacatt
    18721 gactaacgat gaagaagtta cttccattca attaactaag cctaatgaga aggcatcaaa
    18781 aacgatcact cgctctacag ttgctgctgt acttgccgct ctagtggaag aaacggatat
    18841 ttctcgtacg actctagtac tttctgaagg tagcaaagag ttgaatacag cattccaaga
    18901 atgggcaaag gaggaataga tccatgcgtg ccatttcaat caagcatcct ggttctgtag
    18961 atgtattaga acaaattgaa gtgcctgttc ctaaaccaaa acaaggcgaa ttactgatca
    19021 aagtccatac agcagctgtc aatcgaacag atattatgaa aagagaatca gtctctttga
    19081 aacctcccta ccctattttg ggagtcgaag tagctggaga ggtagttgag aataaaagtg
    19141 atatctccac ttttactcct gggacaaaag tatacggatt agtcaatcta ggtggttatg
    19201 ccgaatatgc tgtgatgcct gccgatcgtg caatccttct ccctgatacg ctagactatg
    19261 tttctgctgc tggaatctca gaagtttttt tgactgctta tcaaacactt tactggcttg
    19321 gaaaattaca agaaggcgaa actgtcttga tccatgcagg agcaagtggt gtgggaacag
    19381 cagcgatcca attagctaga cagctttctc acgcgaaaat catcgttact gcaggatcag
    19441 aagaaaaact cgccttttgt aaagaacttg gtgcagatga attgatcaac tataaaaaac
    19501 aagactttca agaagaggta cgaaaaatca ctgaaggtcg gggagtcgat gttattttgg
    19561 atttcatcgg cgcttcttac tgggaaaaga atttagcttc tatagccgtt gatggacgtt
    19621 gggtcttgat tggtatgcta ggtgggacca tcgtgcctga gatagacctt ggtgtattga
    19681 ttgggaaacg tgtccaactg cttggtacat tattgacacc tagaagtgat cagtataaag
    19741 cagagctcgc acaaactttt atgcaaaaag ctgggccttt tctagaaaat ggcaagattc
    19801 ggccaatcat tgaccgtaca ttccaattca gcgcagtcaa acaagcgcat gaatatatga
    19861 aagcaaacaa aaacatcgga aaaatcattt tgaagataac tgaataaaat catgctgatc
    19921 aaaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaagaaat tcacgaaaat
    19981 tgcttttcaa atttttgtga atttcagctt atttccgaag gaatttcttc tgcttccgcc
    20041 gtttatacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctctttt
    20101 tccgaataaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa
    20161 cataagaagc tgtttttact tggctgttct tattacttga tgcagggcag ctttttcaca
    20221 acctcttcac taaacagtgt tcaatcagct gaccctcggt attagctcaa aacgctgatt
    20281 tcacaacctc tttttattta cgttctattt cattcatttt ccgaagcaaa tcgagctgct
    20341 tgtatgcccg cttgtcgccc aaaaataatg atctccgcaa ctgaatttcc accgatccgg
    20401 ttgtttccat gtaagccacc tgtgacttca ccagcagcat ataaccccct aattggctgg
    20461 ttttctttat ctaatacttc agtgttcgta ttgattttca caccgcccat cgtgtaatgg
    20521 attcctggtg cgattttgat cgcataataa ttcggagacg ataagtcatg ttccatggct
    20581 gtggcacggt tgaattgagc atcttttttc tccgcaacat ccgtattcca tccatcaact
    20641 gtttttgcta gtgtttcttt tggtaaatcg attttttctg ctaattcttc cattgtcttt
    20701 ccagttgtga catatccttt ttggtcataa aattcaatag ctgtggcacg atcacgcacg
    20761 ccttggtcaa aaatcaaata agctgatttt tcaggcaatg ctgtaattgc tgcagatact
    20821 ttatcccttg tatccatttc attgacaaat cttttcccct cttgatcgac caagattgct
    20881 ccttctccgc gaacaacctc accaatcaag accccctcat cctgctgcac agttggatga
    20941 atttgtattt tatccatatc aacggtttgt ccacctaatt tttcaatcat ggtgatacca
    21001 tctccagttg atccttcttg atttgtcgta atataatcag ctaaatcggg acgatatttt
    21061 tcaataaatt ctttgctggc accaaaaccg cctgtggtaa ctacgattgc ttttccggtg
    21121 acctcttttg gttcttctcc ttgaacatgt acagtcactt cattgaccgt ttccccattt
    21181 ttcttaatgt ctgtcacatc tgcgttgaca aaaataggga tttcccgctc atggacgtta
    21241 ttcaataacc cctccactaa gtatccacca atagccgagc catcagacgg acgatgtgtc
    21301 ctcttttcac tcatccctcc agtgatcgtt aagttgtcca gtttgatacc gttttgatct
    21361 agccaatcga ccgcatcagc agagtgatcg acaaagtatc gaagcatttc tttatcattc
    21421 gttcctccac caccttttag cgtttcttca tagaaggcat cattcgagtc tttgattcct
    21481 tcttcttttt gaaattttgt ttctgaagca ttcataccgc ttgatgattt agatgtgttt
    21541 cctccggcaa caggcatctt ttccaaaatt actggattca acccagcatc ttttgcttcg
    21601 atagctgctg ccatccctgc accaccagct ccaataatca cgacatcata agattctttc
    21661 atttcttttg gatctgtata tacttgttca gaagcgcctg atgatacttc ggcagattcc
    21721 ttcgtactag aacttgtctg cgctgtattt ttctgatctg cgccgcatgc gctcatagca
    21781 agaagtgcaa cggctgttaa taaaccagca actaattttt tcatttgtat cccccttttc
    21841 tccttatact atattaagtg atttttttca caatatcaac tctgtttttc tttaagtcat
    21901 atagctttgg taaacacacg cttcttagta taattaagga tatacatgaa ggagttattt
    21961 tatgaatcat ttgaaaacct tttttacgaa cagtacaaaa actgcaggta gaatcacaaa
    22021 cggaatctat attttattac gtatcatcgt cgttattatg ctagtggatc aggtctggaa
    22081 ccaacactgg ggaaatacaa tgcttttatt actgacatta attttatttg aaatccctta
    22141 ttttctggaa aagatattaa agatacagat tccaaatatg ctagaaatca ttttgttatc
    22201 ttttattttt agttcaacag ttttaggaga gttaagtgat ttttatggct atttttcaat
    22261 gtgggatact tttctacatg cattcagcgg atttttagct gggggtgtcg gcttttccct
    22321 tgtttatttg ctcaataaaa atacaaaaac catgaacttg actcctttat tactagcagt
    22381 tgtttccttc tgtttttcga tgacagttgg tgttatgtgg gagtttgtgg aattttcagc
    22441 agataaatta ctgcaattcg atatgcaaaa agatacgatc atccagcaaa tcaatagtgt
    22501 ggagttaagt aaagacggaa acactgtcta tcatgttcca gatatcactc ggacaatcat
    22561 tgaaagtcaa aatgcagatg gtaaaacaga taaaacaatc atccaaaatg gttacttaga
    22621 cgtgggaatc atggatacta tgaaggattt atttgttaat tttattggag cagttgtttt
    22681 cagtgtattt ggttacctct atagtcgaaa cgattaccaa aaatttacgt tcatcaagaa
    22741 ttttattcct aaaaaaatca aacgataaaa catgctaaaa aacaagagaa ggggcagtga
    22801 caatactttt gtcactgccc cttctttttt gaatgtatgg catgccatta gttatccgat
    22861 agtattaact caaaaaagta agatatgagg aatgactatt tcgtttttta tcccaatact
    22921 gaggacttat ttcaccattt cgttatttat ctaatttact taaaacctgc ataacttttt
    22981 cataatctgg attaggtgtg ccgtctagat tttcagcaat aaaagtaaaa cccatttttt
    23041 tataaagagc aatcgcctga tggctccatg tctgtgtgtg aagatagatt ggtccgtccg
    23101 tatggagggt aggaaattct tgcaagactc tggcagtcaa tgcttttgcg agcccttttc
    23161 cttggtaatc tggtttcact gctaaccaat gaaaaaccgg atacacttgc tctcttgatt
    23221 tttttcgcca agcggtacag gttgctactt tttctccttt ttttgtctct acaaaaaaca
    23281 tccttctttc aagttcttca ggataaggag caaagttttg ttgaaaatac tcaagtgctt
    23341 catctgtcgt ttgaaattcc ccaacggaag tctcaatctc cgcccaagca gattcatctt
    23401 ctttttgata aaatcgaaat tcaaaacctt ccgctaaagg cgtgattgga acattttgtt
    23461 gaagtggtct tgtcatccag atttctgcgt aaggtaacgt tttatctaac atttttctat
    23521 ccctcctcgt ctccattatg ccaaataaca tatgactcag ctacttattt gactataaca
    23581 gatagagata taaatcagaa ccagtcatct gcgtcaaatt catttcgatt agcgtatacc
    23641 catgttcctg ccatacctcg catgaaatct cctaaagcag aagaaggaat acctccacca
    23701 ggtgcgggtg ttcccggact aggaagtggt acttgcggct gcagatgctc atcggtggaa
    23761 tcaatagttg acaagcgttt tttcttcgtt tccttctggt catctgattc cttctgatct
    23821 tccgactgtt tagcaacaaa ttctttttct gattgttgtt ttattttagg ctctaaaatt
    23881 tcattttcca atccagaaac ttgtggggcc aaatgagctt cttgccttat cgttttatta
    23941 gattctgaga tttttctaga tattcttgct aactctaggc ttcttgtttg ttttactttc
    24001 cctgttggag aaactgcatg agccatttgt tgtttttttt ctgacaagct ttcttcttgt
    24061 cttttttgtt ctttttgatc tatctgtttg tttaatggta aaacatgtaa tagtgactga
    24121 gcatggagtt cttggagtga atacgggtca tgataactat acataatcgt aaaatcacct
    24181 gtttgtttgg tcaaataacc atcctccaat ggtacttcat tgattgtaac cgatacatcc
    24241 tttaacggta cgggtttacc taaggaattt gttgctgatt ggatcaactc ttctttgttt
    24301 attttttcgt ttggatgaac aataagatca tgaccttcta atgaacgatt gtctggtata
    24361 taaacataag taaaggtaat ttcccctcct gattgcttcc tattattcat cagttctgag
    24421 tgaagtactt caaggatctc cgaccagtga ctttttctgt ctaatccgtt tgcttgcatc
    24481 caatcagaaa tagaagtgac tttcccattt tgttcataag tcgataaatc tatatatttg
    24541 gcactcgatc agaaaattca gcaggtaggc gcaaatcagg aatacgattt acactactca
    24601 tatgtgtttc tgcttgaagt tctttatttg tttgactatc tataaaatga gtgatgacag
    24661 tcgatgaagc agcagatgct tctacataaa ctacagctgt tgtttctgcc acatttgatg
    24721 ttttcgagtc tatataagtt aatacaacgg gatattctcc cggagttgta gtatctactg
    24781 tcccgttcat tttgatttct tctttatttc ctgaagttcc atcgcttttt gttacatatt
    24841 caacaagatc tttaggggcg taactctctc ctgttcttaa gtgaagatct tttcctttga
    24901 tagctgtttg atcagcacga acatggatca cagcagatgc agtcattgtt tttaaagcaa
    24961 ttggatcaaa ataggacaag gaaatagtag atgttcctgt attagtcgta tccacgatat
    25021 tcccattgtt tgaaattact ttggacatat ctccttgtgt tccatctgcg ttcttcagcc
    25081 tttctaccag atcagatatc gaaacttttg tccctgccag aacatcgatt ttctttcttt
    25141 caaactgtga ataatctttt tcatatacat aagaaacttg tgcgcttcca ccgtccttta
    25201 tatctgtcaa agcatgattc aaaatatcag tgatctgttt taactgatca ccttttgtag
    25261 gatcagtcat atcggaaaag atagaaagcg gcacactgct tccggtttcc gatgtccata
    25321 atgttttgtt aggaattaac cagtagtggt ttatcgttgg aggataactg actttattat
    25381 aggaagtcaa tgcaggaagt agctgttttc catttgcatc agtgaaagta tgagtaatag
    25441 tgatagggga atcagtggtc tcagaaacag aggtacgtac gaccttgatg gtttgtgtcg
    25501 ttgcctgaac gggtacatgc atatagacac ctacagataa actgatcagc atcgtgcgca
    25561 tcataaactt aacgtagtgt ttttcagaac acgaccgttc cttatctaaa cgacaagcgt
    25621 gtttatttag ttgttttgaa cgtctttttg aacacattta ttcttagtcc tccctactct
    25681 tgtctaaaca tgaagtagta attttcataa gatgatttca ttatataaat aaaatcaaaa
    25741 gaagatggac tattaggaga gtattttttt tgatttttta ttgtttcaag aaaatcggaa
    25801 taataactta tgtacttttt aattttgtta agtaatatat ctagttatat ctagttatat
    25861 ctagttacct tttctttgaa aaaaacataa atcagctaat aaatatgtga gacaaagctg
    25921 ccatccataa atactagctg attttttcag ttaaatgtct atttttttct ctgatgatgg
    25981 tatttccaaa atattcccag tcatctggag tcggtggatt tttccaaatt atctgttctt
    26041 tattaaattt ttcaagtaaa caatcagata aataaatatt cgtacgagaa gtgattcgac
    26101 tctcaatcac aatattgtaa tttttaaatc ctttcacttg agaaataatg taatcggtat
    26161 aagcagatcc ttgcgaaaaa tcaatacaga catgaatcgg tgcttcaaaa tatgataccg
    26221 gacaaatacc gaccaataaa aagagataat cataaaataa gcgaatcatg aaacgttcat
    26281 cttttcccaa gaaatagcgt ttattctctt gtatgacctt ttggattgct gtactgaata
    26341 ttggatacgt ctcgaagaaa aactgtagct gctttttaga cgtaaagcta gtcatctcaa
    26401 agtcgaatat ccaatttttt cgattgacat gcatcatacc catacgaagt tcttttcgta
    26461 tatatagtct ttcttcttca gagatttcaa tcgaaaatgg taattgatca atcaattcca
    26521 ctgacatttt ttgactaatc atgtggatat catgaaaatg agcttccgca agtccaaatg
    26581 cgccatctaa ttcaaattct tcttccgtga tcaaaaacaa catgatataa gaaatctcat
    26641 caatgatctg ctctttaggg atatgaagaa aagaaatatg tgataaatcc ttgatgatct
    26701 tcattccttc tgagctctta aagtattcat ctgaaaagaa acgttttccg attggttgat
    26761 tcccataggc cctcaacaaa ctaatagtgg ttagcaaatt gaatttgatt cgttttgttg
    26821 cagacagttt taaattgaac agatattcaa aatattgcac gatactcttt gcttcattca
    26881 tgacgctttc tctaaatggc atagaaaaac cattaaagac cccgaaatat accccaaata
    26941 atacgttccg tatgtttatt tcttccccaa ctaatatgtt tttctttaac tttatttgat
    27001 gctgttcaag aaaagagact aattctcttc tttttacata ggctcttgat atgcttaaaa
    27061 aatgattctc tgcaaatttt tcgatcgtta cttttgaatc cacgatctcc gttaacagtc
    27121 gagcaatttt tgatcgttga aagtagattc ttcttaaatt tttgatatcc gtcaaattca
    27181 gacgttcgac atggatctct gcttcttctg aaatttggat ctctggcttt gttttgaatt
    27241 cagtaaattc tttatttaat tctatgatat aatttcttgt tttaaattta gacaaaccta
    27301 atgtttcata taacttaggc agcactacat aattcttttt ctcaaattct aaatatctag
    27361 ctagcagcag tttttctcta tcttcctctt ctataaggta atccaacttc atatcctaca
    27421 cctcaaaaaa tgcctcatac caattacttg atatgcagat gaaagttcat cttttcacct
    27481 atactgattt tatgatggat tatgcttgtt ttttcatcag ttattctcat tttttatctt
    27541 ttttttgaga atattgcaaa aaatggaaaa atttatgagg aaatattcta tattttttgt
    27601 tttattgtgt taaaatgtac aagatttgcg ccatttgcgt aaacaaccac aaaaaagagc
    27661 aggacggcca ccgtcctgct cttttactta ttttaagttc cattacttat taattggcta
    27721 aacttctgct tgtttctgat attgcttgtt tttctccaat gatccgcact tctgtttcta
    27781 aagacacatc aaatttttct ttgattactt cttggatgtg cgcaatcaat tcaatataat
    27841 ctgtcgctgt cgcatgatcg atattgacga tgaatccagc atgtttttca gagacttgtg
    27901 caccgcccca tttcaggcct tgcaatcctg cttgttggat cagctgacct gtatagtgtc
    27961 ctactgggcg cttaaagaca cttccgcatg aagggtattc taatggctgt tttgattgac
    28021 gcaattcggt caactcatcc attcttgctt tgattgcttc ataatcccct gtttgcaaac
    28081 tgaatcgagc ttccaatacg atagcacgta attcttggac tttactgtga cgataagaaa
    28141 ataccatttc gtcttttgtc aaagttttca atgttccatc ttcaagcaaa agatccactt
    28201 cagcaaaaac atctttgatc tctccgccat acgcacctgc attcatataa accgctccac
    28261 cgacacttcc cgggatgccg caagcaaatt caaatccagt caatgattct gccaaagctg
    28321 cataagtcgt atcaatcagt gaggcacctg cttccgcaat gaccatcgtt cctttgacat
    28381 gaatctgttt catttcagtc agcatgatga ctacaccgcg aatcccgcca tcttgaacga
    28441 tcagattgct tgcatttcct aataccatcc aaggtatatc atggattcgg caataatcga
    28501 tgatttgctt tacttcttct tgttttttcg ggaatgcaag gacatcagct ggtccacctg
    28561 ttttcgtaaa cgtatagttc atcaatggtt catcaaacaa tatagtaatc gtggctaagc
    28621 tttcaactat ttcttcttta ttcactgagg attttccttt cttttctcaa acgtcctcat
    28681 tattctatca cgcttaatac aatcttgcca gtcttgttca aagttgatga cggtagtgat
    28741 ctttaagcac ttcctcacat aattcatgac ttcttacaac ttctttttgt ttaagcaatt
    28801 ctgtttccga tttacctaaa cggatctgag aaaagaaggc ctctaccatt ggctcaaacc
    28861 cgcgtttgac tagcgtgttt gtccagtcgc caaattcttt cacttttttt ccatcagcat
    28921 cttgaatgac aagctgggtc agatcgttta cttcataagt accattttta cttgttactt
    28981 gatatttttc tatattagcg ccacttgcta aatccatcgt taaaatagcc gtttgatttt
    29041 gtgtttctaa ttgtaaaatg gcatattcca aaaattcctt tgtttctctt atctggtatt
    29101 ttgttctcaa aatgggttca tctaataaat acacagctgt atcaataaga tgtaaaaaca
    29161 aatcataaag gacgaactct gtcgtttctt ttgccgctat tcgatttttt tgcaggtgga
    29221 tcaaccgctt atctgggata cgtttcaatt cttctaccat aggagcaaag cgccggttga
    29281 aaccgatcat caaaattatt tggttcttat cagctaattc ttgcaattca cgaacttcat
    29341 ttagatggac actcagtggt ttgtcaatat aaacatggat accattttct agacattttt
    29401 tggctaattc ataatgtact tttgttgctg cgtggatcat acatgcttcg attttttctt
    29461 ctaaaagctc atcgatcgtt tcatatagat gttggaaccc gtactctttt tgaattcttt
    29521 cttttgtttc tttatttctt gttgcaaaat aaaaatcaag tgttccttgt tttttcgcat
    29581 aagttggtaa atatgctttt tctgcgatat tccctacacc gattacacca atcttcatct
    29641 actttcctcc tggatcattt agttactttc atcttacacc attttttact aaaatgaggt
    29701 acaatagtat cgcaaaggta aggaaattaa ttcgaaggag tgtgctttta ttgaaattta
    29761 tctcttggaa cgtaaacggg cttcgtgcca tcgtaaataa aaatttttta gaagtttttc
    29821 atgagttaga cgccgatttt ttctgtctgc aggaaacgaa actccaagca ggacaaattg
    29881 atttagatct tccaggttat tatcaatatt ggaattacgc ggaacgaaaa ggctattccg
    29941 gcacagcgat ttttgctaaa aaaccagcat tgaatgccac ttacggaatg ggtatcgata
    30001 tccatgatac agaaggccgt ctgattactt tggaatattc cgatttcttt cttgtaacct
    30061 gttacacgcc aaattctcaa agcgaattaa aacgtttaga ttaccgatta gaatgggaag
    30121 aagcctttta taattatttg gaaaatctaa aaaaacaaaa accagtcatc gtgtgtgggg
    30181 atctaaatgt cgctcatcaa aagattgatt tgaaaaactg gaaaacgaat caaaaaaatg
    30241 ctggattcac tccagaagaa agagcggctc tttctcgact gttggataac gggtttatcg
    30301 atacattccg ttatttttat ccaacccagg aaggtgtcta ctcttggtgg aactaccgat
    30361 tcaattcaag gaaaaacaat gctggttggc ggatcgatta ctttttgacg agcaaagatt
    30421 tagagccagg actagctgat gcaaagatcc atacggacat tatgggcagt gatcattgtc
    30481 cagtagaatt agatttgaaa taaagaggtt gtgaaaaagt cgtttagttt cgagtattaa
    30541 gaaaaatttt gtgaaaatgg ctttccacat tttgcataaa tttggcataa taccgagaaa
    30601 ctggcttttg aacactgttt atcgagagat tgtgaaaaag ctgtcctgca tcaagtaata
    30661 agaacagcca aacaaaaata gttcctcata tttttcgttt ggttgggctt attaccgcag
    30721 atacaagctt ttgaacaccg tttattaaga ggttgtgaaa aagctgcact gcatcaagta
    30781 ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg
    30841 cagatgcaag cttttgaaca ccgtttatta agaggttgtg aaaaagctgc actgcatcaa
    30901 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt ggacttatta
    30961 ccgcagatgc aagcttttga acaccgttta ttgagaagtt gtgaaatcag cgtcctgacc
    31021 tgagtattag agaaacaaat ggcaaaatag ttcctcatat tttgctattt tttgagctaa
    31081 tacggagggt cagctgattg aacactgttt agtggagagg ttgtgaaatc agcgtcctga
    31141 cctgagtatt agagaaacaa atggcaaaat agttcctcat attttgctat tttttgagct
    31201 aataccgagg gtcagctgat tgaacaccgt ttagtggaga agttgtgaaa aagtcgccta
    31261 ctttcgagtc gtaagaaatt tttttgaaaa tggttcctca caactttgca taaatttgac
    31321 ttaataccga gaagtcatga tccccatcta actccaaatc acaaggaatg agttcaaaaa
    31381 ctagcttttc tcaacaaata ttacataaaa aattctatgt tttttgttaa atttatttgc
    31441 ttttttcaaa agtatgttac agtaatttcc caaggaggtt ttacagaaat gaattttgtt
    31501 gccatggatt ttgaaactgc caatcatcag ccttatagtg cttgttctct tgcgctagtc
    31561 atggtaaaaa atagccagat cgttgatgaa ttctatacat tgatacagcc tgagactcct
    31621 tttttctggc gaaatgtcca aattcacgga atccatcaag aagacgtacg caatgcgccg
    31681 aaattcccag aagtctggca aactatccaa ccatattttc aaaaaaatcg tttagtcgtt
    31741 gcacataatg ctgcttttga tacaaaggtc ttagcgggat gcttagatta ttatcatctt
    31801 gaacagccta attatctttc tttatgtaca gtgaaaagta gccgtcgcct gtttcctgaa
    31861 tttccaaatc atcgattgaa cactgtgtgc gaaaatttga acatccaatt agatcatcac
    31921 catgatgcat tggaagatag tcgtgcatgt gcggaaatcc tgctttatca agaaaaaaaa
    31981 ttcgggacag atccattgaa aaaacttgtg actataaagt aaaagaatac ttatgaaaca
    32041 cacaaagagg tcgtgagcct gccgtttcac tccgagtcac aaggagcaat ttcaaaaaat
    32101 agcttctcat gtttattaaa tattgtgact ggtgaccgag gagctggcag gagaacaccg
    32161 tttattggag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca gccaaacaaa
    32221 aatagcttct catatttttc atttggttgg acttattacc gcagatgcaa gcttttggac
    32281 accgtttacc aagaggttgt gaaatcagcg ttttgacctg agtattagag aaacaaacgg
    32341 caaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa
    32401 caccgtttat tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc cttcgtgtgt
    32461 ttttctgcct tctcaaaagg atgacagttt ctcactcttt tcctatttct tctattcaat
    32521 caaataactc attttatata catctaaaaa ttcacctaga tctgttcttg cccctctagg
    32581 catgaccgct tcgatttgga agcccatttt tcggtaaagg tgtacggctc tttcattacg
    32641 aacttgaacg tcaagctcca atcgaaaaag tacacccatt tccttcgccc aactgatgat
    32701 ttcttctagc ataagagtgc ctagtcccat tccccaatat tcctgcagaa tactgatacc
    32761 gacttctccc acgtgagaca aacgaaattg actgtctgcc ttgacagatg ctgttccaat
    32821 aatcgtccct tcatagattg caagcagcaa taaattatta ttgctttcgt atatataatc
    32881 caactcttct tctaaggttt ctggtggtaa caggagttct gcttcatcca aaactaaaaa
    32941 ttctgtctcc ttattaactt gtcccatcat caaaagaatg tcttttgcat ctcctggcac
    33001 tgcttcccgc aagatgatcc caacttcttc actcatgatt ttcagcccat ttttctagaa
    33061 ttcgctgttt gaaggtttca ctttgctccc cgtatgagat tagtttcaca taccgctttt
    33121 ctaaatctgt atcacttttt tctaaaatca gttccaaata atcctccggc agtgcttctt
    33181 ctaatagatt tccccattca acaacggaaa gcccatcacc ttcaaaatat tcatctaacc
    33241 ctaaatctgc tccgctagct gctacccgat aaatatccat atgatacaac ggaatccgac
    33301 cttgactata ttctctaata atcgtgtatg tcggactttt gatcatttgc gttatcccta
    33361 atccttgggc aattcctttc gtcaacgttg ttttacccgc acctagatca ccagtcaata
    33421 tcagattgtc acctggcatt gcggaatgac caattgcctc tgcgaattgt tctgtcattg
    33481 ttaatcctga tagttcgatc atttttcatc ttctcccgct cttctttctt tttgccagta
    33541 tatcgcgtaa tgaagttttt ttaaagaaaa aatgaactag tcaagaacag cagctgctgt
    33601 ttttggctag ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg tgataatcga
    33661 taatttatat acatcttctt catttgctcc acgagacaaa tcagaaaccg gtttgttcaa
    33721 gccttgtaga attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa ttttatagcc
    33781 gatatttcct gactgcaatt ctggaaatac aaagactgtt gcttgaccag ctacttctga
    33841 atctggcgct ttcagttgtg caactgaagg aacataagaa gcatcaaatt gcaattcgcc
    33901 gtcgatcatg tattgtggcg ctagtttttt agcgatttta gttgcttcaa ctactttgtc
    33961 tacttctggc gatttcgctg agccttttgt cgagaagctc atcattgcta cttttggttc
    34021 gatatcgaac aattctgctg tttttgcaga atctacggcg atttcagcca attcttgcgc
    34081 agttggattc acgttgatcg cacaatccga aaagacatat ttttcttgat ctcttccacg
    34141 taccatgatc atcgctccgc ttgtccgact gatgccaggc tttgttttga tgatctgaag
    34201 tgctgggcga acagtgtcgc cagtcgaatg gatcgctcca gagaccattc cgtctgcaat
    34261 ccccatatac gtaagcatcg taccaaagta attcacatct tttaaaatct tttctgcttg
    34321 ttctttggat gcctttccat ttctacgttc aacaaaagta tccaccattt catcccactt
    34381 atcgtaatta tcagggctga tgatcgtaaa gctagatgtt ttgattcctc tggcatgcgc
    34441 tgcatttctt atttcttctt cgttccctac taaaatgggt tccacaagtt cttctgcttt
    34501 caaccgggca gctgcgccta aaatacgagc atcagtagct tccgggaaaa cgattttgat
    34561 cccacggcga acaattttaa atcttaaact atcaaacaat tccacaagat gacctccaga
    34621 cgtttttata tgtattatca tacactattc cagaaaaaaa gaacagttta ttttatctgt
    34681 atcagtcaaa aatgctggaa aaattaagtt tttcacatac aaagcgaact gcactataac
    34741 aggaaagcgc tgttttatta cactgtttca ggcagttgcc aatcaatcgg ttcttctcct
    34801 aacgcaagga gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa gccacgactt
    34861 gcagataacg gactgggatg tggagatgtt atgatgatat ggcgactctt atcgatcatt
    34921 ttgatttttt cttgtgccgg ttttccccac aagataaaca cgattggttt ttcccgttca
    34981 tttaattttt caatgatcgt gtctgttaat ctttcccaac ctttcccgcg atgagaatac
    35041 gcttgccctt cccgaacggt aagaacggtg ttgagtaaaa ggactccctg tttagcccat
    35101 gacactagat ttccatgttg aacaggtgcg attccaagat catcataaag ctctttatag
    35161 atatttctta acgatggcgg gatctttacg ccaggttgca cagaaaaaga taagccatga
    35221 gcttgatttt ctccatgata aggatcttgt cctaaaatca ccactttaac ttcttcataa
    35281 ggagtcaatt ccaatgcttc atagatatga tacatatctg gatggatctt ttgggacgca
    35341 tattcttttt ttaaaaattc tcttagatgc agataataat cttttgaaaa ttctgaggac
    35401 aaaacttctt gccaactgtt atgaatgatt tctttcaaaa actccacctc ctaataaaaa
    35461 gaataactca cattttcttc aatcacaagg gacaatcttc accctcagca cttgattaat
    35521 atggtaaact aggaacaaga agcagaaagg aacgataatc attatgatca aattaattgc
    35581 atcagatatg gacggcacat tattagatgc ccatatgagt gtttctcctg aaaatgccga
    35641 ggcgatacgt tttgcaaatg atgccggtgt tgagttcatg gtcgctactg gtcgaaacta
    35701 tcaagaagct cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga tcactttgaa
    35761 cggggcacag gtatttgata aagaaggaaa ttcattgttt accgtgtcga ttccaaataa
    35821 tcaagcaatc tctgttttag atattctaga tgctaacggt atttactatg aagtagcaac
    35881 caacgatggt ctttattctg aaaatcaagc gaaacgaatt gaaagttttg cttccatggt
    35941 ggctacccat ttacctcacc ttacgtataa aatggcgatt gccatggcct cagctaagct
    36001 ggagttattg cacatcacat acgtggacag tattcgtgct attttggaag aaaagaatct
    36061 ggaagtttta aaaattattt gttttcatac agaagggcca gctgtattag gtccagttgg
    36121 aaagcagatt tctcttttag gcgatctagc cgttacttcc tctggtcaaa acaatatcga
    36181 agtgaaccat aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc acgagcgtgg
    36241 gatttcttta gaagaagtca tgacgattgg cgataacttc aatgatgtca gcatgctgca
    36301 agtagcagga gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag attatgccaa
    36361 atatacaacg gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc gcgcgattgc
    36421 agaagacttg tagttttatc tagaaaagag ggatatagat gtcagaattt ttcattcagg
    36481 aaaagcagct tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga cattccctgt
    36541 ttttgatggt tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat gccatgaatg
    36601 gcgatctcgt tgctcacatc aagcagataa gcctgacatt cggtacgcgt tttgagctgt
    36661 ataaagagtt cgaaaaggtg ggaaccatgc agaaaatatt caattggccc ggtgactttt
    36721 attatattcg tagacttcac tggacagtcc aaggtgatat ctataatcat caatatcaaa
    36781 tccatcattt caatcagcaa atcatgaaaa tggataaagc aacacttttt tcaggagatt
    36841 actatgtatt ggatgtctca aatgatgaag atgctccagt ttgtatttgt attgctgcgg
    36901 ttttagatta ctggctctac cgtaaagaca aaagtcgcga taagcctgtt tcttaccgac
    36961 ttaacttaag ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag gtacatcttt
    37021 ttagatattc ttttgcatat gatcataaag atgaccactg atcatccgct gaccgacaac
    37081 tttgaagtca tggcgttcgt ataatttttt agcctttgga tttttttcat ccacacttag
    37141 accgatgacg gagcgattag ctttcttcgc caatttaggt aatgcttcta ataatcgcga
    37201 accgatgcct tgtcctctaa aatcttctga tacactgata gagtccaaat accattcgtt
    37261 gggaaacgtt tctttatcaa tgaacaaacg tgtagacgca tctagctgat atttctctaa
    37321 gataggtacc agtggctgat cgatgattga ttcttcttca tctgtataac cgtaagcaac
    37381 tcctgcaact ttaccgtcta tttcttctac gatacctcgt gaataactgt aacgataatt
    37441 tggatcagtg attgcttctt ctaaaatctc taaagtcttt tgttcccctg tctgcttcaa
    37501 aaatggcagt tccatatctt ttagaatcac taagatcaat ggggcgattt ctggagcatc
    37561 ttcttttctc gcaaaacgaa tcatagtttc ctctcctttt ctccattcta acatgcagaa
    37621 aaagaaatcg caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa tgaaataacc
    37681 atgagtaacg attcttgccg cttcagttcg gcaataaggg cagacgactg gcatttcttt
    37741 atcattatgt tcgactacat tattgacttc ttcgtatgca aatttttttc cgcaattagg
    37801 gcatttcatt ttttccatgt tatctcaact tcctttcatc tttactctct tagcttactc
    37861 ttatattgta aaaaagagaa gcaatatcct tcttctatgc agatatccat acctgacggt
    37921 cctgttctcc atctattaat cggactttat aataaggacg ttcatctttg actagaagtg
    37981 tccactcttt cacttttgct tttttcccgt agtagctgac tgcttccttc atcgctgctt
    38041 gcggttgttt gaactcttcc gttttttcta tatcaaaaag atgttctgcg gccttttccg
    38101 tgtcatttcg tacggttgtt ttcccagtgg atggattgac tgctatgact tgtccttcac
    38161 aaaaaaactg gtattcatat gttgcattcg taggattctc caaatctttt tcaactgcaa
    38221 aatgaatagc agatatgtta tgttcttttg tttcgttttt tacgattgct gctgcttttt
    38281 tcaatgagat ccgatattcc tggtcactaa tatcatccat gtgaaaagcg gcattgagca
    38341 gtcccgtcat accggtaatc cctattacga tgagggcagc aacaccaaac atcatttttt
    38401 tcattcttca ttctcctgtt tctttttttt aaagaatatc aagaaatgaa cgaatgaaaa
    38461 atcgaatgaa acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca gttctaatgg
    38521 caatccgtct ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat cttctctaat
    38581 cggttctgat tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac
    38641 tttgaaagca agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt
    38701 agcaattcca aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc
    38761 tcgttctttg cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc
    38821 acgtgatttc tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat
    38881 atgagcactc ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa
    38941 atgtgtcaga ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa
    39001 cagaaagtag gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa
    39061 attatatttt caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa
    39121 gactagtttt ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact
    39181 acatttagtg tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc
    39241 agctaaaagg ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg
    39301 tcatgactct tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca
    39361 cgcttggtgt attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag
    39421 gtattttgtc tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta
    39481 tcggtgaaca gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat
    39541 ggaatcataa tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa
    39601 tcatcagtac tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg
    39661 atcctcggcc gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca
    39721 gtttcttgcg ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg
    39781 tcctttggta tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct
    39841 ctgtttactt gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag
    39901 aacgtgaacg tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa
    39961 aaaactgagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
    40021 acggcggaat cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag
    40081 caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctatt
    40141 ttttatagta attccttcag atactttctt gcctcatcat tgaaacgcag tgattcttct
    40201 aacttctgat aatattccaa cgcttgtttc tgagcttcag taaaacgata atgttttcct
    40261 ttccatttgt cgattgtctc ttgcacattt ccagattgga tatctttcac tactagttgc
    40321 catttttctt ttgagactgt acggtctttc ttatcagcag attgtatagc ttctcgttct
    40381 ttacgcaata cttttaaaaa accatttctg actatccgag gaatgctgtc gaagaaccct
    40441 tctgtaaaac catagcgaca atattgcttc caaataaata gatagatttt tggttggatg
    40501 aatcgttggt ttttcgcaaa atattccatc tgagcagtga cttctggtgt tcgttgacgt
    40561 tttgtctttc tcaacacgga aataaatgtt tcaaaataag cattaggatg tactggcact
    40621 tcttcatata cgatggattg ccaatcttta cgtaaaaatg cgcgatagtt gatcaagaat
    40681 tcttctacat cctttactaa ttcttctgca aagtcgtcaa atagttcttt ttttatctgt
    40741 cgcttttttg actgcccatt gatgtgttca cttattttct tgatttcatt ttccaatgct
    40801 tgataatccg ctatgtaaat cggcatttct acttggcgaa attctgcgag ccgctgagct
    40861 aattttatat ccggtaaata tggctgtttc atccgacgat tttggtacaa gacaaaacag
    40921 tatttttgcc atagtccttc aggaaaatcg atattcttct gcttaagcca taataattcg
    40981 tctaaggcca gatcaacttt tgtcattccc tgatggatcg aagctgccac tgtcggagag
    41041 acatgcaggt gatcggcaaa gtggttgatg cctagtttta ggatctttcc ggtttttggc
    41101 gatttaacga tgtattgcac ttttaattct ctaccacact cacaatacaa tttttgctgg
    41161 cttcgttttg ttcgaaaaaa cggattgatc ttgaagtcag aaaacaccca atctgttgca
    41221 gctaaataat gttcttccat aaaacgagag ctgattagat atcggcggtg cttatcgact
    41281 agttcccgct gttttttcgt taattttgca tagatctccc aacgttgact gcttgtgtat
    41341 tgtttagaaa ttttgatggg atagcttttg ctgatttctt ttggaaacca ttcttcaaag
    41401 acggattgcc attcttccac ggctgtttct ttcctttcta attacaaaaa gctggctact
    41461 aacataacgt tagcaaccag ccagtgtttc ttgctaaatt agtgagttgt tccggcattt
    41521 ccggaaatat cttctttatt ttctactgct gcttcaactg cataagtcaa agaagaagca
    41581 atgtcttcta aagacataga aggcgtaccc actggacgat cgatcacttg atcgggtgag
    41641 aatggcacgt ggatgaaccc acctcgaatt gccgggaatt gtgtttcgat caaatgaagc
    41701 aaacgataca tgatatcatt gcagacaaat gttcctgctg tataagagat gtacgctggt
    41761 agaccatgat cttgtacatt ttttgtcatt gctttgattg gcaatgaggt aaagtaagcg
    41821 gctggaccgt tttcttctaa agccgtatcg aatggctgat ttccttcatt atcagggatt
    41881 cttgcttctg ctagattgat tgcgacacgt tcgaacgaga ctgctgaccg cccacctgct
    41941 tgtccgacac agatcacgac atctggttgg tgctgttgga tggcttcttt gactacttga
    42001 ctgctttttt cgaatacggt cggtacttcc actttgatga tttcagcacc gctgatttca
    42061 tctggcattt tttttactgc ttcataggct ggattcactt tgtccccacc aaatggatca
    42121 aaacctgtta ctagtacttt catctgttgt tcctccgtcc gattgattta acatatggtt
    42181 atcttttatc ataccatttt ccttgaacag cgtaaataaa ggaaacaata gtttaaataa
    42241 cataacagat caaacttaat atattcttat aaaaaaatag tgttccacta caatgttaga
    42301 agaggttgcg acaattattc tgtcacaacc cctctcttct attatttaaa tagaatcata
    42361 taagcaattt ggaaaactag cataaataac gcaataaaca actggttttt gatcactccg
    42421 tatttgtctt tcatctccag catcgctaca ggcacgatat tgaagttagc agccatcggt
    42481 gtcagcaggg tcccacaaaa accgcaagtc aatgcaacca ttcctattaa cgctggattc
    42541 gctccatgac taaatacgaa tggtgcaccg atcccaaccg tcatcacggt gatggccgca
    42601 aatgcattcc ccatgattac cgtaaatatc accatgccaa gagcgtaaat gatgatgccg
    42661 atgttcacat ttccttctgg aacgatcgta cctactgcat tggaaatcac tgtcccaact
    42721 ccagcgcttg taaataccgc acctagagaa gctaaaagca ttggcaacat gcttaacggc
    42781 cctactgtgc ccagcatatc tgctgcatca tctaaaaatg tacttggtcg attatctcga
    42841 gaaaagaaca tcaagattag catggctgca aaaacaccga cgcccacgcc gactaaagca
    42901 cctaaattgg taaatagagc aaacaagatc gcaaagacac cgatactcaa agctggaata
    42961 aaaattttca ttcctagttt gtctgacatt ttttccatat attcttttga tggaagccgg
    43021 ctttctcctt tgctgacttt cttagcaatt gctggtatgg tcatcgcaaa gatcaagcca
    43081 ccgttcacga ttgccggaag ccatcttcct cctgcaataa ctacccctaa gaccgtccag
    43141 aaaaaggctg acccataacg atgaggattg gttttatctg ttaaatttct tactccagtg
    43201 taaatcaata cgagtcccat cacgatataa aggatctcca gtaatttatc gcctaataaa
    43261 atatcaggat ttgtaaaaaa gtccatctta ttgccccttt cctggataat atttcttcgc
    43321 taatttcttt tctttaagat agaagtagac acttgccaca actaaagccg tgatagccac
    43381 tggaacttcc actaaagcaa gctgtcccaa tgttacgtca tatcctagat ctttcagtgt
    43441 tgattggact agcaacgccc ctgctcctcc gatgaatagt acttggccaa agaaccagca
    43501 gacattttcc atagctgaag ccataccttt tagttcttct tcatgcttag gatggacttc
    43561 taaccctttt gattcgatgg tccccaatgc catcggtaaa atgattggac gaacaaatcc
    43621 agcaactcca ccaaaactca cgttaaaagc cgcaaagatc attcgaaaga tcccataaat
    43681 atctacaatc ataccagcag atactttctt gaatcgcttg atcaaggaag ccgctgattc
    43741 tttcagacca tttctttcaa gcgttcctgt tgccaacata atgatgataa aaatcgccat
    43801 cccgcgattg tttacaaaac ttgtacctaa aagttctaac attccttgaa tgccaagacc
    43861 gccagtcaaa gctgtaacaa ctaaagcaac cattacgatc aatatcgagt ctaatttaaa
    43921 tgcaaacccg agaataacaa tcaagacgcc taaaaggcta agataatttt ccatatattt
    43981 tcctccataa aatgacaata tttttttaat tataaagcaa aacacataaa aaatatagtt
    44041 gttttctcac gaaatagaca ctatttttaa atatttttcg ttattttttt atcacaaaaa
    44101 ttatcttact tattgtaagg taaagctttt tttaatttat tgcccgataa tatatagaaa
    44161 taaggtctag ttactgtttt ttttaaatga ttaataaata acaacttctt tctatgaaat
    44221 tgcaaagcat tcatcttctt ttccgctaac gatatatccg tcagtgcttg gtacacttta
    44281 ctaaaaagta tgagattgcc gatatctgga taggtgtgct atactacaga aaaagatata
    44341 caatctgcca ccgggtagga taaagaggat gatcattcca ttaggtcttt gaagggatga
    44401 ttttcctctt tttgtgtatt tttttatagt taaggaggaa tgtaagtatg gcatggatgg
    44461 aattaatcat agcaggaatt ttggaagtat tctggtcaac gatgatgaaa tggagtgatg
    44521 gtttttctaa aatcaattat tctttttaca cagtgattgg catgattgct agtttttact
    44581 ttttgtcaaa ggcaatcaaa agtttgccga tgagtctctc ttatcctatc tggacaggga
    44641 ttggcgcagt tggttcggtc ataattggtg tggtcttgtt tcatgataag ctgaatcttt
    44701 ccacttggtt ctttgtgggg atgctactca tcagtatcat tggcatcaag attactagcg
    44761 gccactagac acttggaata agtactgatc agaaaaaata agccttttta tttaatattc
    44821 aattttttta tgaaaaaata agaaataagt ccaatgacct agcgacttct ccactcatca
    44881 ggtgtatgct tgtactatct atagaaaagg agtgaatatt tttgaaaagt ctttcggtcc
    44941 aagaattatc caagaacttt ggtcacaaac aagccctcag cgatgtcagt tttagcttat
    45001 cccctggcaa aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca acgatcatga
    45061 aagctatttt aggactgatc cattactcag atggaacaat ctgtattggt gaaaatgaga
    45121 tttctccctc tagtcataaa gggttggaac aagtaggagc attgatcgaa tacccgggta
    45181 tctatccatt cttaacaggc tatgatcatc tgaagttatt ctctgaaacc aacgatgtat
    45241 ctgctatcga cactatcgtg aatcagctca agatggaaaa gtacattcac aaaaaagcga
    45301 aatcctactc gttgggaatg aaacaaaaat tgggaattgc tcttgcctta ctgaaccatc
    45361 cgtctatcgt tattttagat gagccgatga acggtttgga tccacaagca acaagagatg
    45421 tgcgaaaaat cattaccgac ttggctactc agggcacctc tttcttgatc tccagtcact
    45481 tactcagcga gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa ggaaagatca
    45541 tcaaacaatg tacaatggct gaattgaacc aaacagcgca agatttcatc gttatctcta
    45601 caacaaatga tctagctgct cgaacgatct tgacagaagc tggatatact ttggaagatc
    45661 ctgataaaat caagctttta aaagaaaacg aagaattatt aggaaccgtg atccagctat
    45721 tgtctgcaca aaatatccat gtgacagacg tccagcatct gacgaatgat ttggaatctt
    45781 ctctattgga aattctggat tcagtaaagg gggaagaaga aaaatgacat tgcttgtaaa
    45841 acaagaatta tttaaattga tcaagaaaaa gtcaactgct gttttatctg ttctattagt
    45901 tgttttgctc ataggaacag cacttctagc caaaaaatac acgacgatca ttgatccagt
    45961 tgaaatgact gctcagcttt ttagtgccac atcttggatt gtctttatca tgattgcagc
    46021 tgcaagcacg atcatttcga tggaagcaca atatggtaca ttgaaaaatc tcctttatcg
    46081 aaagtattct cgaggagaaa tattagtaag caaatggatc acattagtta tttattctgt
    46141 ttatttatac cttttagcta ttattgtcac tgtgttgatg aaactgattc ttttcccttc
    46201 gatttccttc acggagaagg tttctactgg tcaaacactg atccaaagct tgttcactta
    46261 tactttgggt agttatatcg gtttatggct gattttaagt ttagtgttga tgattgcttg
    46321 ctttatcaat agttccggtg catccatctc agcaggaatc gtcttttatt ttgcttcgtc
    46381 aatcatatct ggtatcctta ttgcactcat ccaaaaatgg gagtggatca aatggaatcc
    46441 gatcagtatg ttgaatctgc aaaaccaaat tggaaacgaa gagatcatga agcaattgac
    46501 tcatctttct actaatcaaa tgctattcgg taatttagct tacatcgttt tgttcttagc
    46561 attaggatat ctcgtattta agcgaaaaaa tgtatgatga ctcatcaaaa aagaggctgt
    46621 gaaagagacg ttcagcctta ttgccgattg gctgtctctt aaacaccgtt tattctgaaa
    46681 aaggagctgt gacaacattt gtgtcacagc tcctttccta gtatgttttt gaataaacga
    46741 caggctcaca acctctcttt atacttcttt tatacttcta tacgtgaagc atcccagtgg
    46801 caaataaaac cattgcaatc actccgacca tcgtcacacc tataagtaaa atcttatcta
    46861 agtggatatg ttgtacaggc tcgttttgct ccttacgtgc tttgaggtaa aaataaatgc
    46921 ctggcaagaa aagaacaaga acaagcatta cttcattaaa ggctacgact agcatagccc
    46981 atacttgaaa ggcacttgct aaaagcccta ctaaaaattg gacccattct ttccgctgat
    47041 aagaaagttt catttggtac aatcctaaga aaagccacgt gaagaaaata gctgctgtac
    47101 caagtgtgta agcaaagtta taagcactgt cagtgaataa taatgaaagt aaaaagacat
    47161 tcgtacataa cgtcgtcaat acgatagcat aagttggtgc cccatttttg ttgagttttc
    47221 cccatgcttt aggcaaaagt ccgtcttcag ccatgtttcc cattgtttca atcggtaaga
    47281 tcgtccatga gagccaaaca ccaactaaag aaatgatcaa accgccgttg atcacgactg
    47341 ctccccattt accaaccatc atttcaaaca catagcccat agcaggctgc gccttgatcg
    47401 tttgcagctg attcatcgtc attgtcccat atggaagcat ggaaaccaag acgtaaataa
    47461 taatcaagcc gaatatccct agaatcgttg ctttcccaac atctgattgt ttttttgctc
    47521 gtgaacctac gacagaggct ccttcaatac caacaaatac ccaaagcatc accatgatgc
    47581 aactcaatac ttgactggaa aacgggatcg gctgtccatt cgcattcaat ataatgtcgc
    47641 tagaaaagtt atcaatgaat tgttggatcg aaaacgtgct gatagctact acaataaata
    47701 aaaataatgg cactaattta cagaatgtga cgatagcatt gacgaaacta gcattttcga
    47761 ctccccgatt cactagccat gcatatcccc ataaaaagac tgtactcatc acgatcgatg
    47821 ccagattttg cccaccttca aatactggga agaaatatcc taatgcgctc atcaacaatg
    47881 ttgcaaaagc tacgttgcct aaccagcatg tgagccagta accccaaccg ctgataaaac
    47941 cagcgaaacg gccaaaacct tcttctgcgt aactaaagat cccgcctttt aaatctggac
    48001 gttttttatt gatattattg atcgacaagg agagcatcaa cattgctgtt cctacgagta
    48061 cccaactgaa aatagctggg ccgccagccg atccggagat gtccgtcata ataccaaata
    48121 cgccacctcc aatcgctgaa ccaattacta aggcagcaag tggaattaaa ccaattcctt
    48181 ttttctgttg ctctgtatcc atgtaagcat cctccaaaat aaaaccgctt ttatcaaaaa
    48241 gtataacaaa ccttttttat ttttacttaa ttaataaaaa ctttaataga aatataattc
    48301 taccggcttt tttctcatta tacaagcaaa gcgcctagtc cctttccaaa ttacaccacc
    48361 aaaaaactta aaaaagatgc tatactaaaa ggaaagaatt ggaggatgat ccatgaacat
    48421 tttatatata tcgatttcag gaaatacgcg tgcctttgcc aaacatctag cagaatatgc
    48481 tgaaaaaatg catgcagaag atcctgtcaa tcctgaagtt actttaaaag aaatccatga
    48541 aaatagtgat tttacgaaag aaacagaacc attttttact ttcgttccta cttatttaga
    48601 cggcggaaat ggtttagata acggtgatac agaaatcttg accgaaacga tgcgtgaata
    48661 tctggaacat gaagacaatc ataaactttg tcttggtgta gtcggtagcg gaaataaaaa
    48721 cttcaataat cagtattgtc taacagccaa acaatatgca caaacatttg gctttccttt
    48781 cttggcagat tatgaattac ggggaacacc atctgatgtg acacgaattt atcagatttt
    48841 agcagaaaac aataaataat aaaaaagaag aagctgaaaa atagatttca gtttcttctt
    48901 tcgtttctat tgtttctatc atattaagct ttgacatcca tcacaggtgc ttttgctttt
    48961 gctttacctg ttttttccaa gacaaagttt tttacacgat ccatattggt taccaagacg
    49021 atcatggatg tttctttgcc tgctgctttg atcgcttcta agtcagcttc tgctacaagc
    49081 gtatcaggtg tcacttgctg cccttctgtt actttcacat caaatggcgt accgcctaag
    49141 tcaactgtgt tgattcccat atgaagcaag atttctaaac catttggcgt tgtgatccca
    49201 atagcgtgtt tcgttgggaa gacagagctg actaccccag taactggaga atagatatgt
    49261 ccgttttctg gttctacaaa gtagccttca cccatcatct tttgtgcaaa aactggatca
    49321 cttgctttat ctaaagaaac aactgttccg tcagcaactg atgagaggat agtcacactt
    49381 tcagctgaag tttgaggtac tactttttca ggcacttcat ttactgtggc tgtttcagtc
    49441 gctaattctt ctttttctaa acggtcttta ggaataccga agaagaatgt agcaacgaag
    49501 ccaccagcat aagctgccaa taatccaagg atgtagccta accatttacc gtcagagatc
    49561 aatgggatca aagcggctcc tgaaggtcca atagcgatag ccccgacatt tccgatcatt
    49621 ccgatcactg ccccgccgat acctccaccg atacaagcag taataaatgg gcgtcctaga
    49681 ggtaatgtta ctccgtaaat caatggttcg ccgattccta ggatcccgac tggtaaagct
    49741 cctttgatca tttctgttaa ttctttatct tttttgcagc gaatccataa tgccaatgct
    49801 gcacccactt gtcctgcacc agccatcgca agaattggaa gcaatagcgt acgaccagtt
    49861 tcatcaatca tttgcacatg gattggagtc aaaatttgat gcagtccgaa cataaccatc
    49921 ggcaagaata atgtgcctag aacgaagccg gcaaatgctc caccaacgcc aagtacccag
    49981 ttgatcccac cgaccatgcc atcagaaatg aaacctgcta aaggcatgat gaagaaaatt
    50041 tcaatcagac cgatggcaat caatgaaaga gttggtgtaa cgatgatatc taccgcatca
    50101 ggtactactt tatgcagttt tttctcaacg attgacaata gccaaacagc gaagatgaca
    50161 cctaggatcc caccttgtcc agctgcaaga gcttcaccag taaataagtt tttcagtggt
    50221 gcttccggat tcatccctgt cagcatcaca acggccccga taactccacc aagagttggt
    50281 gttgcaccaa aaacttgtgc agcattgata cctgtataga taactaggta gctaaacatc
    50341 ccgtttttca agatgttcat cacatcgata tattgttgcc atgttgctgc atcaagtgtt
    50401 ccggctgtga ctaagtttgc taaaactgcg gcaatccctg ccacgatccc agtaccgaca
    50461 aatgcaggaa tcattggcac aaagatatta gaaatgtctt ttaataccga tttgatttta
    50521 gatggttttt gtttcgattt ttgtgccgct ttcatttctt gtgcccgttc gtttacacgg
    50581 tcttttccag atgctgatga atttgctgta gctgtactta cagaagaaag tttttctcct
    50641 aatttcacgc ctgccaaatc aaccatttgc tgtgctactt tgttcacttt cccaggtccg
    50701 atgatgactt gaagctgttc atcttctact acaccaagca ctcctggaat cgcttttaat
    50761 tcagagacat ttacaagatc atcattgatg atcttcatac gcacacgagt catgcagtgg
    50821 acaatgcttt ctacattgcc catgccgcct gcccctttat aaatttgttt tgccagttct
    50881 tccaaagtca gttcgttttt tgccatggtc ttttccccct cataaggtcc cttttacaaa
    50941 tccgtttgct tcagctaatt ttgcttgagc ttcctcttta tcactatttg tcaaaatcat
    51001 gacgatagcc agcttaacat tttgatctgc ttcttcaaag tacttttcag ctgtttcgta
    51061 atcagcttct gtcgcttgca tgatgattcg ttttgagcgt tcgattagtt tttcatttgt
    51121 tggtttgaca tcgaccatca agttattgaa tactttacct ataccaatca tactgatagt
    51181 agaaatcata ttcaagataa gtttttgtgc tgttcctgac ttcaatcgag tagagcctgt
    51241 caaaaattct ggacctgcat cgacttcgat tgggaaatcc gcgtgtttac tgatttcggc
    51301 ttgtttattg caagaaatcg ttcctgtgac agcaccgatc gattttgcgt aatctaatcc
    51361 accgatcaca tatggtgtac gaccgctagc tgcgatacca ataactatat cattttttgt
    51421 caaattcagc ttgattagat cttcttgccc tagctcataa gaatcttcgg ccccttcgac
    51481 agctacagtc attgcttctt ctccaccagc aattagacct atgaccattt ccggttctac
    51541 accgaatgtc ggtacgcatt cagcagcatc taacacacct agtcttccac ttgttccggc
    51601 tcccatataa atcagacgtc cacctttttt aaaggactca atcgtgttag caatcactgg
    51661 ttcgatactt gtcagttctt tttcaaccgc agcggccact ttctgatctt ctttgttcat
    51721 tagttgaacg gcttgagaaa tactcatctc atctaatcca aaagtttctt ggtttctgcg
    51781 ttccgttgtt aggttctcta aattgatttt gctcattttt atcttccttc ttttcttata
    51841 aattgatagt tcataccagc tttgatttgg ttgatcaatg gtagctcttc tttgataact
    51901 ttagctacta cattcacttt ttcatccgct ggcaaatcat atttggttaa ttggatctca
    51961 cccatatagc gcaaatactt ttcgttatct aaagtaatag accctttcat acgcgtagct
    52021 gtatttcttg ctgggatagg cggaatctct cgaaaacgtg catctacgct tcgtatgaca
    52081 tctcttgcat cgtcttgccg attcgtatgc ttccctaata tatattcgta aaattcttca
    52141 tctacaggtt ccgtatggag caagactgtc tgttcttttt ggaaagaagc aaattgctcc
    52201 tgaacgtttt ctgacagacc tgcatctccg atgtaaacaa tatccgtgtt acttgctaat
    52261 agatctagag cgcctgcaag aggatggatc cctcgatgtt cttccaaagt tggcaatcct
    52321 tgatatagtg ggcctctcaa ctctgtatct cctggtacaa aaccttggac aaggagacct
    52381 tgaattttga gccattgatt ttttttctga taccattctt tgtccagccc tgtttcggga
    52441 cgtggataat aattatgcca agcttcaatc tgtgagaaat cagcttctgc ttctttcaat
    52501 tcatcgatat ctttaggcgt gatcgtgcta gcattcaaac tgattttcaa ttgatgtgac
    52561 cagtcggcaa tctgctgatt ggaaatatga taatccattc ttaaacctgt tacaccaatt
    52621 gctttcaaag gcgttagatc ttctaatgaa aaacccgctc gttccaatgc ctctcctgaa
    52681 atatcgacca tcaattctag tttattcttt ttcgcttcgc ttcctagttc gatcaatcgc
    52741 ttttgatatg ccgaaacatc atcttctggt atatgcatgg aagtgaaaat accgacaaag
    52801 ccattggatg ccatttggcg gatatactgt ttcttctctt ctgacaaaga atcgttcata
    52861 aatattgaaa agccaaacat attatccctc tttttctgta tccgtcttca tttctatgac
    52921 tagagtatat atctaaagaa acaaaatttc aatactttta ttaaaataat taaatttcat
    52981 ttctaaaggt tgagtttata ttatcaaaag aattgtcata acctaaaaaa ggcactgcta
    53041 agcaaaagca gtgccttgaa atattttatt taaataacac aaaaacataa ttactcataa
    53101 gaaaccaatt ctgtattatc taccatgaat gtaaagaacc cctcattagg catcggatga
    53161 aaataagcat tgatcactct tatcaaggca ttatggcaaa ctaacagata gacattttct
    53221 tcgtcttcga tgcattcttt caataaagga acgatccgtg catacacatc taagactgac
    53281 tccccatttg ggaatcgtac agcgaacgct aaacgtgctt tttgaaagtt ctcatctttg
    53341 cttggcagtc catcataatc cccaaaatcc atttctacca atcgttcatc aacggtcagc
    53401 ggcaagctga gacgatcagc aactgcttgt gctgtatctc ttgctcgttg aagaggggaa
    53461 tggatgattt tcgtgattgg aacaggcaaa tctactacct tttctgctag tttctgtgcc
    53521 tgcatctgtc ctttttcagt cagcggcaca tctgcccgtc cgcaaactaa tccttgtgcg
    53581 ttccaagttg tttctccgtg tctcgttaca taaagcatgt gattctctcc ttcgttggca
    53641 aagttcccct taaccatacc ttgttgttct tgagattaca agggttagat tctctcaaga
    53701 gaaataatct cagtagatac tttatccagg aaggtttgat catgttccac tagtaacatg
    53761 gcaggtttca cgttcaagat cagttgttct aattgttctt gattgaagac atccaaataa
    53821 ttcaatggtt catcccatgt atatagttca gctggctgtg ataaagattt agccaattcc
    53881 acttttttcc gttggcccat actcatctgc tcgatcttgt tatgaaaaac atctctttcc
    53941 atcccaagct ttcggaggtt gttcaaaaat gcttggtagt ctacttggtt tttctctgca
    54001 aattccgcca acgttcctcg attgtcttca taattttggc ttgcataact aatgctcaga
    54061 tgttttggct ggtatttttc tcctataacc ttgccgttga atgcccccag aagataatgg
    54121 ataatggatg actttcctgc accgttagga cctgaaatcg ccacccgctg atgaggctcg
    54181 attgtaaaat gaattggctc gaataacaga ttttcatacc ctaattgaag atcttctacg
    54241 cttaaaagtc gcttatggtg agacgcttgg ctattcatcg tcaaagaatc gatatattcg
    54301 atatctttta atagtttttc tttctcgctg atctgggtct ccatccgatt cacgattgct
    54361 ttggatcgtt tcatcgtccg tgcagcatca gcacccactg ctcctttatt cactcgtcta
    54421 gattcagtat cgatgaatcc gacttgtttc tttgttttat ctccttctcg ggaacgagac
    54481 cattcggctt tttcagctgc tgttttcttt agcctactga cttctttttt caatttttca
    54541 ttttgagcca tttcaaattc atcacgaagt tttttctgtt cttcatagat agagaaattc
    54601 ccttgataaa gttccagttg acttttttcg attgctaaaa catggtccac tacttcatcg
    54661 ataaatcccc ggtcatggct gaccacgatg aagccttgtt ttttcttttt caaataagcc
    54721 gctacttgtt ttctaccaga gatatccaaa tgattcgttg gttcatcgat taacgggaaa
    54781 tgagtgtcat ccacaaataa aagtgccagt aggacttttg tcttctctcc ccccgatagt
    54841 gtgctgaatt ctctccataa gatttcagga tctgtttgca tcaattggag ctctctttcg
    54901 atttcccata tctcaaaatc cgtaatatca tttaacacgt aataggttaa acgttcttta
    54961 tcttttgtct gttgcgggaa ataggcaaat tcttgctgat ggattacttg cccttgataa
    55021 ggtagtttgt tttgtagaat attcagtaaa gttgtctttc ctcgaccgtt tcgtccgata
    55081 agtcctagtt tccattgtgt gtcaaaattt agatttgctt gttcaaataa taatgtgcct
    55141 tggctgtcgt agccgaatgt cagatttttt atttcgattt tagacatttt ccatcacctc
    55201 gtaaaaaaag accagtccta ttttcagaac tggctaaaga ggatgcaagg gttggataca
    55261 cacaaaaaga cccatacatc gaaaaaacca agccaatccc gaaaatctgc acactgcttc
    55321 cttgtctcca caaggtacag tctgaatgca gctttcatca atcgacttaa tttttcaatg
    55381 cacggatccc tcactttgtc ctatatttga aattacgata gcataaagaa aatgattttg
    55441 tcaactactt cgggaggatg gtgaaaaatc tgctaagtgt ctgtcctaag atttttgttt
    55501 tttctaatgt ttgcctgtgt tccattgctt cttctaaacc tattggaccc aaatgaatac
    55561 ttaagacaat tgggagaaaa tcagaaagtc cttctagctc ttggtctcta cttccgcaaa
    55621 tagcgattat tggttttcga tatttttttg caagcactgc tacaccgtat ggaacttttc
    55681 cgtggatcgt ttgctgatcg atccgccctt ctcctgtaat gatgacatct gcttgttcga
    55741 tctttttttc caatccagtg atttggctga ctaaatcgaa accagaaacc atcttcgctc
    55801 ccaacaaaaa aagggctcca ccaataccgc ctgctgctcc acttcctgga atatcatcta
    55861 aatccacctc ccagaaatct tttatttgct gagcaatctt tcctgcttgt ttatctaaga
    55921 aagctagcgt cgtatctatt cctccttttt gtcttccaaa ttgccaagca gctccttgtt
    55981 gacctgtata tggattggta acatctgcag cgataatcaa ttttacttta gaaacaagaa
    56041 cgtttcttcg atcaaaatga agttttttag tagataataa aggatttcct tctgtgtacc
    56101 cttctaaaga agcacccaag gattggagta atcctagacc accatcgcta cacccgcttc
    56161 ctcctaaagt gacaataatt tgttctactt cttgaagcag tgcatcttta ataagttctc
    56221 ctaaggaata actggaagca agtcggatag tttgatccgt tggtttatca agtagatcta
    56281 accccaatgc ttgtgcagat tcgatgactg cggtgttttt tccttgccaa gttgtcaata
    56341 aatagctgat ttttcgctga cggaataaga gatccatccc atcaatggta cgccatgttc
    56401 ccctgagcgc gtaatagatt gcttctgcag tattttctcc gccatctgca atcggcacat
    56461 tgatttttct tcctttacga tcttcccagc catttagaac tgcctgattt agttcaaatg
    56521 aagaagcact tcctttaaat gaatcaattg ccgccaaaat atccatctgt atccctcact
    56581 ttcttcttaa gtaagacact ttttttcatt tttttcaaac aaaacaatgc tattttcttc
    56641 catttttcta tatactaaaa gggaactcat tgaataaagg agagaaaaaa catgaaaatc
    56701 gcaatagctg gggccggagc aatgggcagc cgaatcggat tgatgctaca tcaaagcgga
    56761 aatgaggtcc tcttgatcga tcgttggcct gctcacattg aagccattcg tacaaatggg
    56821 ttgattgctg actttaacgg taaagaagtg gtcgcaaaac tgccaatcta ttcaccagaa
    56881 gaaatcatcg aaagcaacga acacgtggac ttgatcgtag cattaacaaa agccaatcaa
    56941 ttagatgata tgttttgttc gatccaatcc attattactg acaatactta tgttttgtgt
    57001 ttactaaatg gattaggtca tgaagatgta ctggaaaaat atgtgccaaa gaaaaatatc
    57061 ttatttggga tcacgatgtg gacagctggt ctagctggtc ccggaaaagt cacattgtta
    57121 ggtgatggag aaatcgaact agagaacttg gaaccagaag gcgaagcatt tacaaaaaaa
    57181 gttgtagaag tctttcaaga agcaaattta aatccgatct atagccataa cgttcgttat
    57241 tccatttggc gaaaagcttg tgtaaatgga acattgaatg gtttatgcac gattttggat
    57301 tgtaatattg ctgaactagg cgcgcaaaaa gcggcagaga gcatggttcg aacaatcgta
    57361 tctgaatttg cttctatcgc agcaaaagaa ggaatcatac tagatcaaga agaagtgtac
    57421 caacatattg cttctactta tgatcctgac aacatcgggt tgcattaccc atctatgtac
    57481 caagacctga tcaaaaacca tcgtttgact gaaatcgact acatcaatgg tgccatttgg
    57541 cgaaaaggac aaaaatatga tattgctaca ccatattgtg cctttttgac ccaattagtc
    57601 catgcaaaag aaggaatcct tggcgcagaa taaaaggctc tctgatgaga atcagctgaa
    57661 tcagcagctg gttctctttt tttaagccca ataaaagatc aaaaagaaaa cacaactctc
    57721 atgatgccat aagaattgtg cttgtttgtt tcagtacata atctcttcaa aatgtaggtt
    57781 ttttatttgt tttcaacaga ggagaggtca tattcgtatt gctcataaat agtggaatcg
    57841 ttagtgataa atcgttttgc ccaattttct gcatcataaa attccaatag ctctgatagg
    57901 attggcttaa atggtgtatg cagaaattcc ggtaattcat ctaaatcacg ttctcgaagc
    57961 aaaaaaatat tttcaggacg ataaaaatga taatgagcaa tcgcttgatt atccgtcact
    58021 agtttctttt gaaaatacat ggattcaaat gtccgcaaag tcactccttg ctgaccctcc
    58081 cgcaacagtt ccagaatccc gcgcgattcc cctgtttttt tcaggtactc ttcataagta
    58141 agatatccag gttgttcatt ccctcgatgt ggaagaatga aatagttggt cgtaacatct
    58201 ctttttataa attcctgctt atagctttct gctcgttctt ttctcccatt attcgttgca
    58261 ccaaaaaata gatccgtctt tggttcgcta gaagaaaaat tcatatgttt cgaataaaat
    58321 gaactattat gtttcagccc aagttctttt gcttccaaaa aatcaaaatg ataaatctca
    58381 tcgattgccg gttcatcttg tgcctgcttc agcatcgcct cgtgttctgc tttgaagtga
    58441 ttccaaaaaa ataacacaat ttttgcattg gtaaatgggc gcacgcgtcg agccatctcc
    58501 atatcaaatc cataatctaa aaaaacgatc agctgataat ttgccaactg ttgtctagct
    58561 gatttaatga attccgactg tagtaaatct tttccggacc aaacatctaa agagcggatg
    58621 gaaggcaaaa aataaagtga tttatttttt gcaactagca gtacgtcatt tttgttcatc
    58681 ttttccgcct cactttctct gtcacttttt cctatgatag cctgtttaaa tagtaaaaag
    58741 ccagtaaaac gtttcctata tttgtatgaa aatttaacat ttgtatgaaa atatgatgaa
    58801 ataaatcatt cttctattaa aattcgccta aaagttttct gaatagttgt aattgtctga
    58861 aaatttgtta tcattagtca ttgttaaata ttgctattta ttcttaatat aaattttgat
    58921 tgtgaattta aaagaaagag gtcatttatg gaagataaaa aattgaaaaa acaacttagc
    58981 tccagacata tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct atttaaagga
    59041 agtggcgaag cgattgggat cgccggccct tctgtattga ttgctttttt gattggtggg
    59101 ctgattttat atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg
    59161 catcacggat tatctggttt gatacgccct tatctaggtg ctcattctgc tgattttact
    59221 gattgggtct actggtctat gtggattatc aatatcatcg ctgaagctgt tgcagctgct
    59281 tcctttttgc agttgtggtt cccgcacatt ccagcctggg tatttgtttt tatgttggct
    59341 gttttaacta cgatcatcaa tctttattcg gtacggttat tcgcagaaac agaatattgg
    59401 ctggcttttg ctaaaatcag tgtcattatc ttactgatca tttttgctac ttatcttgtc
    59461 ggtcagcaaa tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt
    59521 gggtttactc ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct
    59581 tacggcggtt ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg
    59641 attcctaaag caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg
    59701 tttttgcttt tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt
    59761 gtgatggtat ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt
    59821 gtccttgctt tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc
    59881 ttccgtttga aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa
    59941 gtaccgcaac gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct
    60001 tattttgtcg gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta
    60061 ttgatctgga tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta
    60121 tgggataaag gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc
    60181 ctgttcacga actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat
    60241 ttcagttatc aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa
    60301 attttatttt atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt
    60361 cttgttgcaa ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca
    60421 aaaaccctct gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg
    60481 aatgaaaaaa aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg
    60541 tgtttcaacg gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat
    60601 cttcttcagt cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat
    60661 catgctgttg ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc
    60721 cgctagtctt cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa
    60781 cgacacagct tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa
    60841 acgagctgat ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca
    60901 agaagaagag atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt
    60961 ccaaataaaa aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac
    61021 ttctggaacg ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag taataagaac
    61081 agccaaacaa aaatagcttc tcctattttt cgtttggtta ggcttattac cgcagatgca
    61141 agcttttgaa caccgtttat taagaggttg taaaatcagc gttctgacct gagtattaga
    61201 gagacaaatg gaaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc
    61261 agctgattga acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa
    61321 gaaaaatttt gtgaaatggc tttccacatt tttgtataaa tttggcttaa taccgagaaa
    61381 ctggcttttg aataccgttt attcgtaaaa gggattgtga caaaagtctt gtcacaatcc
    61441 ctttttatgt ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac
    61501 aaattttttt gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag
    61561 caaactgaaa aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac
    61621 gaaacttccg gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag
    61681 cgttctacct aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt
    61741 accaggcaga ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt
    61801 catcaatgtt gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat
    61861 cacggttgag atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat
    61921 caatgacaca gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg
    61981 ttggtttcca gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga
    62041 agtttgtggg ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt
    62101 taaaattgcc ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga
    62161 ccagattgaa atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag
    62221 acttgcggct ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc
    62281 cgggacagtt gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc
    62341 tgctgctaga tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga
    62401 aacaatagct ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa
    62461 tgcaatccca atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc
    62521 acacacaaca aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac
    62581 cattagagtt ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat
    62641 aaaaataaag attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca
    62701 atctgtcggg tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat
    62761 cgtgatatta ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac
    62821 cattaataca agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc
    62881 tcctttttta gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa
    62941 ttcatccgcg attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg
    63001 actccgcggt accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca
    63061 aatgtatgga ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg
    63121 actttctgaa caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac
    63181 cttccataag atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat
    63241 ttttataact ttcacacaca agtttatttt ttactagaaa gtagcgccct cttgtttttt
    63301 tcttgctact atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa
    63361 cagatcaaga gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt
    63421 gtcactttta gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat
    63481 tgctaccgta ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc
    63541 gagacttgtt ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg
    63601 ctggaagaat aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa
    63661 caatccaaac tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac
    63721 tttttcatat actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc
    63781 agcgtaatct ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt
    63841 gacatctttt ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc
    63901 ttccaaagca tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa
    63961 tttgatgtcg actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg
    64021 atggacgcat aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt
    64081 agctgatccg ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc
    64141 agtttcatca atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa
    64201 attatgcccg catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat
    64261 tgtgggaccg ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac
    64321 ttctttttct acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata
    64381 aaactcatag ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc
    64441 tggtaaacga tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa
    64501 aaattcctcc tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac
    64561 gcttgatcct tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc
    64621 tactattttt tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac
    64681 ataaggacgt gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa
    64741 tccagcatct acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg
    64801 tgctgtttgg gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc
    64861 agaaacagtg ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa
    64921 taacagaaga gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc
    64981 tttgatatca gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt
    65041 atttccgatc gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg
    65101 atgttggaaa gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta
    65161 atctgtaaat ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt
    65221 gtcccataca tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc
    65281 agttgtatct gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat
    65341 gactacaaaa cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt
    65401 taatcagttg attactgata aattgactta aaaaaactaa gattagaatc aataatgtag
    65461 ccacaatcgt tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta
    65521 atccgcctgc acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca
    65581 acgccgagac ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac
    65641 cgattggact ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca
    65701 gtgcgttctc gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag
    65761 ctgctttcgc accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa
    65821 gcaagataat gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata
    65881 gcagacggtt agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga
    65941 ttccaatcat cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg
    66001 cttcgacaaa ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag
    66061 tcattttatc ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa
    66121 atatttattt ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt
    66181 tctccagcta aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg
    66241 ccatataaaa tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg
    66301 ttcgtctgat ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca
    66361 aaagcagaat gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata
    66421 aaatctttgg tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca
    66481 acgattcttc catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc
    66541 tcatgagtga tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca
    66601 aggatctgaa gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc
    66661 ttaggttcat tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt
    66721 tgtttaggat aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact
    66781 ttttgacgac gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca
    66841 aaaatagttc ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt
    66901 tcttttcgca attctttggg agagagctga ctcagattcg tctgattgac aatgacttct
    66961 cctgctgttg gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct
    67021 gagtaaccga cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact
    67081 gcctgtatct ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt
    67141 aaaattcctc cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag
    67201 gacgcttttt tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc
    67261 aagtacgatc atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct
    67321 cgggatgaat tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc
    67381 gaagctctct gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa
    67441 aaaaaaagtc cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc
    67501 accttttttc ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt
    67561 cgttttaacg gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc
    67621 cattttcagc ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc
    67681 aaacttactt tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg
    67741 aaaactgaaa agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt
    67801 tttatttaac atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt
    67861 ttccttttct tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc
    67921 taatcctgct cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga
    67981 actagcttct aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt
    68041 ctccgacact tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc
    68101 caagatatct gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg
    68161 ttcttttaag tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt
    68221 cagctgttga taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct
    68281 acctgctgct ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc
    68341 aacgatttct tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg
    68401 atacgttttt tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta
    68461 tacattaatc ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag
    68521 aagcaaagaa ccagccttct cgtttataag agttcttgga atcgacggat atagattttc
    68581 ttcacagctg tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa
    68641 ttgccaggaa gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa
    68701 ccgacaataa ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata
    68761 aatggtactt tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg
    68821 acaaaccatc ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta
    68881 tggaaacttc cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta
    68941 atatcaaata tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca
    69001 ttccattttt ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg
    69061 caggaaacat catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat
    69121 ggcaggaacg tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta
    69181 gccttgatat atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc
    69241 aaaatcgtca aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca
    69301 gctgtatcta cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca
    69361 tctcctaaaa atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt
    69421 tgctgaggat tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga
    69481 tcactcattt ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga
    69541 cggcacactg aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca
    69601 tgttctttca gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca
    69661 agataaccaa tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt
    69721 ggattggtct tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact
    69781 tttgcaagta cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca
    69841 ataaatttag aaatgttcag catttcttca atcgcgcctt ttgtgatcat ttgtgtttta
    69901 ccagctggat cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca
    69961 tccacttttt gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata
    70021 gccacatcta acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc
    70081 aaaacacgat catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct
    70141 tgtgtcaatg tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg
    70201 gcattcaaat ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa
    70261 ttcgtcgtaa cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca
    70321 aaaagaaaag cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga
    70381 gccatcaatg acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag
    70441 cttgattgaa ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt
    70501 ccaacagaaa caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca
    70561 agattttcgt aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct
    70621 actggatagc tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat
    70681 aaacgcatat ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg
    70741 atttcttcca taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc
    70801 acaagtgatt tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt
    70861 gtcccgctga cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca
    70921 ggagctgcta atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat
    70981 gtaaatggcg tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat
    71041 gaaatcgcat tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca
    71101 tttgctgatg catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca
    71161 tagatttttt gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac
    71221 agctgttctc gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa
    71281 aggggctgag aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa
    71341 tgactgtcat ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa
    71401 tcatttttcg aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg
    71461 ggatttgcgg caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat
    71521 attaaaaaaa gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca
    71581 gatacctgct tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat
    71641 tggcgtctct cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa
    71701 atcttattcg gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt
    71761 tccatgtttt tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt
    71821 atttgtctca ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt
    71881 atggaaagaa cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc
    71941 atcatccaac gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg
    72001 actcctgaat tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa
    72061 ctgatcgatt acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg
    72121 atcgatatcg tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg
    72181 atccgaggag actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt
    72241 gatgctgcgg taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct
    72301 gaaaaagcgg cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat
    72361 ggccagcagt tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac
    72421 tgtgaagaat tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct
    72481 ttgataataa tctgccatca atgtttcata gatcactgta cttctttctt tatgtgctaa
    72541 gctccgtttc atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata
    72601 aaacgaagtc aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc
    72661 taatcgtcgc attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa
    72721 tatccaagga aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat
    72781 caagtattct tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac
    72841 ttctgttttt tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt
    72901 aaaatgccta cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg
    72961 atctagcatt cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca
    73021 ttcatattct gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga
    73081 catttttttc actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc
    73141 cgctaattgg gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc
    73201 atacataata gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta
    73261 aaaaggaaaa atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa taccaacctt
    73321 caagcattag aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt
    73381 ttgctgttca acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt
    73441 ttttgaaaag acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc
    73501 cgcttcattt ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata
    73561 aaaccgcaag agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt
    73621 ttcctgatct ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc
    73681 tgtctgatta ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc
    73741 atttcgaaaa gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag
    73801 ataatcttta tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc
    73861 accgtattca tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac
    73921 tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct
    73981 tgctaaatct ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat
    74041 tgaattttgg taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc
    74101 cagataatgt gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg
    74161 aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt
    74221 cgtgtaaaac ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt
    74281 atctaatatc ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa
    74341 attgtcataa cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc
    74401 atggaaaaca gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa
    74461 gacattgtct gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt
    74521 ttccaacata ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc
    74581 aaagctgatc gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc
    74641 gtctaccttc atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt
    74701 tttcccttta gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata
    74761 atctaaaact tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc
    74821 tgttttgatt ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga
    74881 acggtcaaat gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc
    74941 tgaaaaaata agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt
    75001 gcttttgata ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat
    75061 atccagccag tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc
    75121 ttgcgacact ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg
    75181 ataaaaaata atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat
    75241 agctaacgct ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt
    75301 cgcaattggg ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa
    75361 atctgcatag taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat
    75421 tgctaaacga ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc
    75481 acattttttc ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa
    75541 gattttttta ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg
    75601 atgtaagcga ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg
    75661 tattcaaaag ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt
    75721 cgcagctcct tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt
    75781 tctcctgcca actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt
    75841 tgaaattgtt ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt
    75901 gttcaatcag ctgaccctcg gtattagatt atgtcaggac gctaatttta caacctcttg
    75961 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatataa
    76021 ggagctattt tttgaaattg ctccttgtgg ctcagagtta aacggcaggc tcaagacctc
    76081 ttgatacacg gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg
    76141 aggaactatt ttgccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
    76201 ctgtttttca ttgtccatag gattgatatt gtccttgtga tgactcattc gtaataattg
    76261 aggcttgatt ttcttcactt cccgttatcg tttgtccact gccagaatct gaaggacttg
    76321 ttcctgtcga ctcgtttttt tgagaagaga cagttgtttt cttgacttgt tcatttgtct
    76381 ttttccattc cgctaaacga tcggcgacca cttttttgtc tactttttca gaataagcgg
    76441 tgatcacttt tccatttttc aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa
    76501 cggtcaaatg cttgtcttta aattgatatt gtctgagaga aatcgcacta agtacgtcat
    76561 cgatattaat aaattgattt tcttcttttc cttttggcaa ataaatgtcc aattcgcctt
    76621 tttcgtaatt ggcagttatt ttatactccg cccaccaata agttgagaat tcttttttga
    76681 aattatctac tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg
    76741 ctgtcatttt tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc
    76801 aaaagagaag aaacggtctt tttttcctca tgctttcctc cactcctatt taattttgac
    76861 tattctatta tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat
    76921 aattgaactt ctttttcaca aaatggtaag aatggagaat ttcacgcatt tgctctcaac
    76981 tattctttct taagcctcaa atccgttttt atctgatacg tctttgaacc aatgacccga
    77041 ttttttgatt gttctttttc catcttgtgc taagtctact gaaataaagc catatcgatt
    77101 cttatatgca ttggtccacg accagttatc catacatgtc cacatatgat agcctaaaca
    77161 attggcacct tcttgaatgg cttgatggac atatttcaga tgagattgga caaattcgat
    77221 ccgataatca tcgtggatca tgccatcttc tttgataaat cgtgcttcgt tttctacacc
    77281 cataccgttt tcagaaataa agcagtcgat atttccataa tgatctcgta cattgatcaa
    77341 aatatcatag attccttgtt catagatctc ccatccgcga taaggattca ttttacgacc
    77401 aggcatttca taattgtcga aataatcctc aggcattgga ccgtttttag tatcgattgg
    77461 tgtttctttt gcttttaccc ggcgtggctg gtaataattg atacctaaaa gatctactgt
    77521 attttctttg ataaccgtca aatcctctgc atggatcaca ggcatgagat caagttcttt
    77581 tactatctca atcaattcac ttggaaactc gccttttaca gctggatcaa ggaatgaacg
    77641 attgaagaac gcatctgcta tttgcgctgc tttaacatct tcagggtttg attcgtctct
    77701 tggatagctt ggtgtcagat tcaagatgat accaataaca ccaccttgct ccatttcatg
    77761 gtaagatttg atggctaacg ctgaagccaa cgcttcatga tagccaactt ggatcgcttt
    77821 tttcatatct gactcttctg gataatgcca tcgatataaa taacctgctt cgactggaac
    77881 gattggttca ttatgagtaa accatttttt tactttcgaa ccaaacaatt caaaacaact
    77941 tttggcatat ttttcatagg cttcaaccgt ttctcggttc aaccatccgc ctttttcttg
    78001 cagatacatc ggcatatcga aatgatacag attcacaaag ggttcgatac cgttttcaat
    78061 caactcatca aaataacgat gatagaaacg aacggcttct tcgttgattg ttccagattc
    78121 atcaggtatc aaacggctcc attggataga tgttcgaaat gaattatgcc cagtctgttt
    78181 catcaactgt acatcctctt gatacttcgt ataaacttgc gaggtttttt caggtcctac
    78241 ttgatcaaag aacttttgcg gttctttttt gtaccaataa tcaaaaatgt tctctccttt
    78301 tccatcgcct gaaatccttc cttctgtttg tgggccactt gctgcagaac cccaccaaaa
    78361 atttgctgga aattgatatt ttttcatcaa agcacctctt tttctttttt agttcgtttt
    78421 agttgattcc atgtatgata ttttccatca caatcacttt ttcattttct tcctcttctt
    78481 ttttgattcg ttttaacagc tgttctgctg ctcgttcccc gattttatct gttggtggcg
    78541 tgatcgtgat tttcggaagg atactcagat cgtttttatc taaataatca tagtgactga
    78601 acgcaatatc cttcccgatt ctgacacctt cttgattgaa acattcgatg gcaccaatcg
    78661 acatattgta attgctgaca aaaatagcag ttgccccttt ttctaataat gcttttgccc
    78721 cgtaatagcc gctttttcta gaatagtctc ctgtaaagac ctctaattgg atcgatggat
    78781 cattttggac cgcttctttg gctcctgcta aacgttctct tgctacgtag tctgtttgag
    78841 tggcagcaat aaatccgatt tttttatgtc cttgttcacc atgatgagcg atcacttttg
    78901 ctacactttc tcgatcattc actaatattg tatcaatacc tgctggtcct tctggacaat
    78961 tcaatgcgat aactggaata ctcatttttg tgagcatttc aaatccagac cactcttctt
    79021 cggataagaa gacaatcagc ccatcgatca catgcttcat caggtattca attttttgtt
    79081 caattttttc tggctgatcg gcaaacccgc ttaataataa gctataccca ttttcttcag
    79141 ctactctttc gatccctgag acaacttccg acccaaaacg gctggaaatc ccgtttatca
    79201 aaagtccaat agagaagctt cgattattct tcaacccttt agcaatgatg ttttctttat
    79261 agtccaacgc tgaaatcgct tctgctattt ttttcatgtt cttttccttc agttgctgac
    79321 cattcaagta acgggaaact gttcccactg aaacaccagc catcttagcc acatcacgga
    79381 tcgatgccat acattctcac tcctgccagt tacatttgat ttctctttct tgttgttcga
    79441 ttatgatgac cgcattcttt gcatagtgtt gcttgtcatc aatatccata taactaaagt
    79501 cttcagtaga gcaacttcct ctcactatta attatactta aacaagtatt tgaaccggtt
    79561 catttttaat atagcatttt tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa
    79621 gtggcggatt ttttgccttt ttaaaatttt attctaaaat ttactctatt tgtgggtgtt
    79681 ttgtgcgttt ttctcttcca tccgtttgaa gttttacatt ccaatggtat aatttcttta
    79741 tcctatcaaa tgggggaagt attttgaaga aattaatcgt actatttatt ttgtctgcgg
    79801 gacttttgac attgactggc tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa
    79861 caaccgattc ttctgtctta aaagcggaaa aagcagatac caccaacagt gtatatacgc
    79921 taaaagtacg taaagatatg gatttatccg cgggacagtt gacatttaca gataaaaaaa
    79981 tggaatggac acgcacgtat cagacacaag gaactgattc ttctgaatca tctgaagaaa
    80041 aaacagtgct gacagatatc aagatcacta ccaaagatga cgaatatatt atttcaggaa
    80101 aagaaaataa aaaagaaaca acagtcatat ttacaaaaat cggtaattac cgtttgaagg
    80161 atgaggatgg caacatctac agtctatagt gccgtctgtt ggatcttctt ttgtttctcc
    80221 ccttcttttc tgctatagta aagaagcaag atgattgtaa aggagacgaa atggatgatc
    80281 gatagtcctg attattgtac atgtaataac aacaaaaata tccatacaga atttaatgac
    80341 caaggaagct tgagcatatg cgacaactgt cacaaactag tcaaagattc atttaaaaca
    80401 tttgaatctt atcctgttca aaattatgta aaaagtagaa gaagataaac aaatccaagc
    80461 aaaactcact atggctgatt tttatgaact tcaaactcag cttcttcctt tgatcaagct
    80521 tcatcagtaa aacaaacagt ggatcatttc agtttacaaa tcaaagagta aagatttatt
    80581 tatttcaaat atttgaatat aaaataacca aacgatagaa aggcttattc tacatcacgt
    80641 agtaattttt ctttcccgtc gtttggtttt ttatttttag cctaattctt gaccttttgc
    80701 atgctggtta tccgtaaaat attttccagc aggctttcct tccaattcag ctaatttttc
    80761 ttggatctga cggatcacct tttttgcatc ttctggattt tctacaaaat cgaatttatc
    80821 cccatccact tgtattttag gacatacatc aaaatcttca taccattgat tatagcgggt
    80881 gttcaacgtt tcataatagg tatataacgt ctcatcgctt tctagctgtt catagtcacg
    80941 cccacgtttt ttgatacgtt ctagcatcgt gtccaaagaa actttgatat ggaccatcaa
    81001 atcaggccgt ttttttggag caacatcatc taactcgttt agcatcgtat ctaataagct
    81061 atcgtactgc tgtacttcga tatccgttac ccgacctaaa tcagcgttta gatggaacaa
    81121 caggctatct tcatagatgg atcggtctag cacattgtca tcgttcacta aagcattttt
    81181 cattgctaaa aatcgcttgt tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg
    81241 atttgaataa aagagcggca atacttcgtt atcttcgaca ttttcataaa atggtttaga
    81301 atcaatctct tttgcaagca tatccgtcaa actgctctta cctgcaccaa tcgttcctga
    81361 aatcaaaatc actgacatac aacccttcct cttctataag tactcgccta cgtaagtttc
    81421 aacaggcaat gtttcaaaat catattcacg tacagcttgg tctgatttta aatacgtatg
    81481 gagactttgt gtaatcatca agttcgtagg taccgtctct tcttgtacaa tgatcatcgg
    81541 ttttttcaac atagttgcca cacctaattc gtaagccgtt cctggatcga ttgtttgatg
    81601 ctcaaagtca aggatagcaa caacaaattc tgcatttgtc agttcttcca tatctttttc
    81661 gtatacttcc tgcgcccatc cagcgctgaa tagttcataa ttgctttctt ggtgatgacg
    81721 tggagagtag aaacttgtga ctgttttgtt ttcctccaac gctttttcga tacgacttac
    81781 tcgatcgatt tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac tcattatttt
    81841 gcctccttca ataataactg atttttaata gatactgctc gtaattcatt gaccagttcc
    81901 aatggattgt tggaaaagat tacttgatcc aaccattttt tatccataaa atcctctttt
    81961 gcaaaagttt ctaataattg ttgcaagccg ctgtaataac cgccaatatt caatattccg
    82021 attggcttgt cgggcgtcag accgattgct atccaagaaa gaagttgtga gaattcctcc
    82081 aatgtaccta gacctccagg gaaaacgagg acagcatctc ccttttcaaa taataatacc
    82141 tttctttcat cgatcgtttc tgtaggaata aatgtagtaa catcttttcc aggcaattca
    82201 gtttcaaaaa gcccattcgg atagatacct gtgacttttc cgttattttc cagtactgtt
    82261 ttactaatca ctcccataat cccggagtat gaaccaccgt aaaccagttc gatttcttct
    82321 tgtgccatgt attttcctaa cgtttgcgca ataaatttgt aactttcttt attaccaaaa
    82381 cgggaaccgc aaaaaacagt aagtttcata aaaaaacact ccctaactca aaagtctatc
    82441 ttcattctac acgtgttttt ttaaaataaa agactagatt ttgtagtttt ttagtggtat
    82501 ataggataga cgccactata tatagtattt agaaaaaaat catttttcag ccattttata
    82561 gataaaacaa aaataaataa cgtgttttca tcgatatgaa atgacttact tatagaagat
    82621 actgggattt cgtcctatat gccaataacg tgtattattt taaacaacaa atacttgttt
    82681 cttttattaa cgaaaaaatc attttgttct cgtgttatgt ttcactgata aatgatgttt
    82741 ctctaaataa aaaagccgac gaactaaacg atcaaaattt gatcattttt gttcatcagc
    82801 ttctcctaaa aatctttttg ttaacgataa atagctggat ttgtaaaaat atcacttgaa
    82861 tcgtcactgc tggaagaaaa ttcagtgata accgcacctg ttgcgcctgc ctggaactag
    82921 tgtttgatat tgggctcgaa cgtgtactga tctctagcat gaagaataat ttcttttttt
    82981 actgtataat aactttcttt cctttttgga atagtacagg aaagtttttt tgtttccttt
    83041 ccttcgacat aaggtatacc agaccctttc gacaacgaaa ggttctcatt tttccttcat
    83101 cgtcatttca aggagggtgc cgatgctctg gacagtttcg atcgctccta aaccaaaatc
    83161 tgtatattcg attctttcta actctgattc tttcatacca ttcctgtctc aaggtacatc
    83221 tattgtactt ttttcttgta atccattctg cctctcctaa ctaattgtca aatcatccca
    83281 agatatgtct tcttcctctt tttcttcttg gatgatttct gcattcggat ctactggttt
    83341 tttcaataat aaaatggtta ttcctgtgac aaaagaacca gctaacaatg caccaatgaa
    83401 tgcccatggt ctggtcatcg tagcgactgc aaacattccc gatgccggaa tacggctatc
    83461 tgcgccccaa aacatgctta atccgccacc tactgtccca ccgactcccg ttgctatgac
    83521 attgcgtgcc aagtcgttca atacgatagg gaaacatccc tctgtgatct gacagatccc
    83581 cataggaaaa gcggtcttca atgtatcgat ttcttgacga ttgagtatat ttttacgaat
    83641 gattttccct aagaaataag cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat
    83701 caatacagtg attggttcat agattccttc tgaaaatcgt gccacacccc cttatccaga
    83761 ataaatggta atccagaaac agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa
    83821 tatgagaagc tatttttgtt tggctgttct tattacttga tgcagggcag ctttttcaca
    83881 acctcttttt ttatttatgt ttcataatca atatggtaag gatgcagctc cgtatggtag
    83941 accgcctccg aatattcaat catttcccca atttgcgtag acgatttccg tatccgtttg
    84001 atgccgatct gtgcgtctgt tttcagaatt ccttgttctt cttctgttaa aaatactgcc
    84061 gagaaatcat cagaaaatga gtcaatagta tatccttttt tcgtcagcaa acgatacaaa
    84121 gattctttgc caaattcttc tctgctgaat tgcatcattt cttgtggaag ataatgaaca
    84181 aaataaatat aaggctgatt gttcaaataa tataagcgct taaaacaaac agcttgctca
    84241 ccgaaaaagg aaaacaaagg atcttccttc tttatatgga ccaattgcaa atccagcgtt
    84301 tctttccgga cttcatatcg ggagttctcc aagatttgcg taaagcttgc cgctttggat
    84361 aatcgattgt aaggacgatt gcttagaacc gtcgtcccac gaccactttt ctttgcgaca
    84421 tattcatctg atgccagcag ttcgattgcc tttcggacag tgattttgct gacttgaaac
    84481 agttcttcta attcactttc tgtcggcaac atcgaaccga ctggatattt tcctgagaaa
    84541 atatcttctt taattttatc tgcgacttcc aagtatagta cacgtgtcct tttcataacg
    84601 tcaccctttg ctttactatt actaaaagaa tagcacataa aaaataaaaa aagaacacaa
    84661 atcacatgtg agaagtgtgt tctttttcat tcagctgctt taattacaag tttacgccat
    84721 ttgaggcaat tacttcttta taccaatcaa aagatttttt ctttgatcga ttcagtgtcc
    84781 catttccttc atcgtctaga tccacataga taaagccgta acgtttactc atttctcctg
    84841 ttgatgcact gacaagatcg atacatcccc aaggcgtata gcccatcaaa tctacaccgt
    84901 ctgctactgc attagccatc gcttcgatat gacgacgtaa ataatcgatc cgataatcat
    84961 caataatcgt gccatcctct tccatatgat caatggctcc aagaccgttt tcaacaatga
    85021 ataatggttt ctggtaacga ttgtataatt cattcaatgc gattctcagc ccttctggat
    85081 cgatctgcca gccccattca cttgcctcaa gaaacggatt ttttactcct cccagcaagt
    85141 tcccttctga agttgctgct tgtggatcag tctcattgat tgccgtagac atataataac
    85201 taaatccgat gtagtccact ggatattctt ttagcaactg gaagtctttt tccgtttgct
    85261 ctaatttttc cggatcgact ccatattttt tatgggtacg cgctgtgtat gcagggtatt
    85321 ctccacgtac ttggacatcc gtgcagaaaa aattgaattc ttggttttgg atcattgttg
    85381 ctgcctgatt gactggattg gcatcgatgc tgtaagttgt tgcatagata atcatgcagc
    85441 cgatttggat atccgaacgc aattcgtggc cgatttttac ggctttgcta cttgcaacga
    85501 attgattatg ccatgcttgg aaaatatttt ggtactctcc tgctccattg ctttttacta
    85561 atccttgact taaagctgga aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat
    85621 atttgacttt actgctgtat ctctctaata ctgtctgagc aaatcgttca tagaagtcga
    85681 tcagttttcg atttttccag ccaccgtatt cttttgccaa atgaagaggc atttcgtaat
    85741 gagaaatcgt gatcacaggt tcaatatcat attttaagca ctcatcaatc agctgatcat
    85801 aaaattctaa tcccgcttcg ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc
    85861 aggcaatcga aaaacgataa catttgaatc ccatttccgc aaacaaggca agatcttctt
    85921 tgaaacgatc gtaatgatcg attcctcgat gattcgggta aatatatttt tcttgatcaa
    85981 tcgtccagtc aaattcttca ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag
    86041 catctgcaac ggatagtcct tttccgtctc gatcataagc cccttctaat tgatttggca
    86101 gcagttgctc caccccataa aaatttttct gaaaaacgtg attgcatcct gatttcctcc
    86161 ttattttaca attgccagaa catcttcgtt tgcaataatc tctttttggt ctaattcaag
    86221 tatatcggca aatgcatcag tattcgtaat gacaactgga gtgattacgc tatagccagc
    86281 tgctctaatt gcttcgatgt caaaacgaat caataaatct cctgctttga cgagatcccc
    86341 ttgtttgact aagatctcaa agccattccc atttaattct actgtatcca tcccgatatg
    86401 aatcaaaatc tccgctccgt cagtcgttgt gatccctaca gcatgtccag ttggaaaaat
    86461 agtcgtgatc tctccgtctg caggtgcaac tagttctcct acttctggat caatagcgat
    86521 accttttccc atcacgccag aagaaaaaac ttggtctggt acttcactca atggcagtac
    86581 tttacccgtt aaaggacttt ttaaggtatg ccgtgctgaa gtaatcggtt ctcctgcatt
    86641 cgcatgtttg ttttctaatt gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt
    86701 acctacgaat gcaagaacaa aagcaattcc tgtagctatt actgctactg tcacatttga
    86761 ctctacacca tccaccgtat taatgaaagt cggaatcgtc aataagctga ccaaactgct
    86821 ggagaaagct tttactccgg aaaatccgat aatagctcca ccaatacctc cagaaataca
    86881 agcggcaatg aatggtttct tcaaaggtaa tgtcacacca tacacagttg gttcagtgat
    86941 tccaaataac gaagtcactg tagaagaaat ccctaatgct cgaagtttcg tatcttttgt
    87001 tcgcaatgct acagctaaag cagccccacc ttgtgccaag atggctggaa gaagcattgg
    87061 catcaataca tcaaacccat attgttcgat attcaaaaac atgatcggta cgaatcccca
    87121 atgcatgccg aacatcacga atacttgcca taaacttccc atgattagac ctgctacgat
    87181 tggactaaat ccataaatcg agttgaagag accgcctaaa gcattaccgg caaccgtccc
    87241 gattggtcct aaagcaagaa atgttaatgg aaccatcact actaaaacga caagcggaac
    87301 taaaatcatt tgcagaaatt gcggaatcac tttttttacg aatggctcta atttactttg
    87361 aacccatacg gctagaatga ttggtatgac agaagaagta taaccagatg gtgaaagaat
    87421 cactgggatc ccaaagaaac tgatagcttc tcccgcacca gcaactgctg tgatagctgg
    87481 atacacaagt gccatagcaa ttgctactgc taaaaactgg ttggctttga actttttagc
    87541 tgctgtaaaa gcaagcatga caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa
    87601 tatttgataa gtaccgctat cagctgtcag ccagcccatc accgttgcca atgataagaa
    87661 tcctttcaat accccggcgg ctgccatcgc ccctagaaaa ggtgtaaata tactagagat
    87721 aatatcgatc agacgattca aaatattttt ggactcttct tttccatcct ccgaaactgc
    87781 aagaccttcc tcatccacga tcgattgata aacatctgct acattactgc caatcactac
    87841 ttgatactga ccgccacttt gtacgacttg gatcacgtcc gggtcttctt ttaacttttg
    87901 cgtatctgct aaagattcat cttttaatct gaatcgcaac cgcgtagcac aatgtactaa
    87961 acttttgaca ttcttttgtc ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt
    88021 atccattgtt ttttccttct ttctttttta tttaaaaaat aaaaacccag attgaaaaaa
    88081 acagtcacaa acattcgtga acctattttt tcaatccagg ttttgcctgc catacagtaa
    88141 caatccttat gattggatgc tttttttctc acttactact ctttgaatat gaatcgttaa
    88201 ataaatctgc tctgcgatcc ccatttcctg tttttgcgtt tgtcgaagat actcattgat
    88261 ccgttcgaca caatgaaacg cttttggata ctttccttga actaatgcgt agagaaattc
    88321 atcttgctcg tcagatcctt cccctttgag ataacgctgg gcaaaaaact gcagatgagt
    88381 aatgatacgc tgataattca aagattcttc atcgaacacg atgccgaaga atttactgat
    88441 gatactcagt atatctctta ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt
    88501 tccttgttgg ctattcacaa aatgaaaggc aataaaaccg gcttcatcct cagaaagttc
    88561 cacacccgtc ttttgtttca cgaacaaaag agcctctttc gctgcttgat attctttcgg
    88621 ataaaatttt tttgtttcaa ataataatgg attaggcagt tggatatttt cttttgctcg
    88681 tgtgatcgca taatgaagat gatcggtcaa tgtcagataa atattaggcg aaaattcaac
    88741 ttttagttta tcttcagcca gttcgactac ttgtttgacc cattcgatct cctctgccgg
    88801 aatatcagcc atcagctgct ggaattggtt tttagcaaaa gaatctctta aaacaaattc
    88861 tttttggacc gcttctggcg aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt
    88921 tcccatacaa acgacttctt ccccgaatcc atttttcgaa atgacgacat tgttattcaa
    88981 aattttatca attttcattt tgcttcgccc tctcaagaaa aataaaaaaa cctagaattc
    89041 gcctactgtt tcttagctga attctggttt tgcctgccgt acagtaacaa tccatttctt
    89101 attcaaaatc atcataccat cgaattttta tttatgcaag ccttttcata taaaaaataa
    89161 aaaaactgga acacaagtct atctagaaaa caa
//
LOCUS       DACDNO010000003        85549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDNO010000003 DACDNO010000000
VERSION     DACDNO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 85549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.003"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..171
                     /locus_tag="IWI22_000178"
     CDS             <1..171
                     /locus_tag="IWI22_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9167603.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            346..642
                     /locus_tag="IWI22_000179"
     CDS             346..642
                     /locus_tag="IWI22_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167604.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="IWI22_000180"
     CDS             649..2601
                     /locus_tag="IWI22_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9167605.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IWI22_000181"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="IWI22_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9167606.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3532..4572
                     /locus_tag="IWI22_000182"
     CDS             3532..4572
                     /locus_tag="IWI22_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9167607.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4670..4858
                     /locus_tag="IWI22_000183"
     CDS             4670..4858
                     /locus_tag="IWI22_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9167608.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4916..5269)
                     /locus_tag="IWI22_000184"
     CDS             complement(4916..5269)
                     /locus_tag="IWI22_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9167609.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5800..6222
                     /locus_tag="IWI22_000185"
     CDS             5800..6222
                     /locus_tag="IWI22_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9167610.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6219..6449
                     /locus_tag="IWI22_000186"
     CDS             6219..6449
                     /locus_tag="IWI22_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9167611.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6947..7147
                     /locus_tag="IWI22_000187"
     CDS             6947..7147
                     /locus_tag="IWI22_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9167612.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7174..8367
                     /locus_tag="IWI22_000188"
     CDS             7174..8367
                     /locus_tag="IWI22_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9167613.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="IWI22_000189"
     CDS             complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="IWI22_000189"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9167614.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10059..10982)
                     /locus_tag="IWI22_000190"
     CDS             complement(10059..10982)
                     /locus_tag="IWI22_000190"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9167615.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11178..11783
                     /locus_tag="IWI22_000191"
     CDS             11178..11783
                     /locus_tag="IWI22_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9167616.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12091..13107
                     /locus_tag="IWI22_000192"
     CDS             12091..13107
                     /locus_tag="IWI22_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9167617.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13248..14549
                     /locus_tag="IWI22_000193"
     CDS             13248..14549
                     /locus_tag="IWI22_000193"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9167618.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14595..15257
                     /gene="deoC"
                     /locus_tag="IWI22_000194"
     CDS             14595..15257
                     /gene="deoC"
                     /locus_tag="IWI22_000194"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9167619.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15278..15676
                     /locus_tag="IWI22_000195"
     CDS             15278..15676
                     /locus_tag="IWI22_000195"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9167620.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15742..16824
                     /locus_tag="IWI22_000196"
     CDS             15742..16824
                     /locus_tag="IWI22_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9167621.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17036..18598
                     /locus_tag="IWI22_000197"
     CDS             17036..18598
                     /locus_tag="IWI22_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167622.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18591..19724
                     /locus_tag="IWI22_000198"
     CDS             18591..19724
                     /locus_tag="IWI22_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167623.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19724..20680
                     /locus_tag="IWI22_000199"
     CDS             19724..20680
                     /locus_tag="IWI22_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167624.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20779..21945
                     /gene="deoB"
                     /locus_tag="IWI22_000200"
     CDS             20779..21945
                     /gene="deoB"
                     /locus_tag="IWI22_000200"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9167625.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21969..22787
                     /locus_tag="IWI22_000201"
     CDS             21969..22787
                     /locus_tag="IWI22_000201"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9167626.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22804..23511
                     /gene="deoD"
                     /locus_tag="IWI22_000202"
     CDS             22804..23511
                     /gene="deoD"
                     /locus_tag="IWI22_000202"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9167627.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23809..24495)
                     /gene="gpmA"
                     /locus_tag="IWI22_000203"
     CDS             complement(23809..24495)
                     /gene="gpmA"
                     /locus_tag="IWI22_000203"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9167628.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24667..25248)
                     /locus_tag="IWI22_000204"
     CDS             complement(24667..25248)
                     /locus_tag="IWI22_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9167629.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25381..26064)
                     /gene="rpiA"
                     /locus_tag="IWI22_000205"
     CDS             complement(25381..26064)
                     /gene="rpiA"
                     /locus_tag="IWI22_000205"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9167630.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26383..26796
                     /gene="rpsL"
                     /locus_tag="IWI22_000206"
     CDS             26383..26796
                     /gene="rpsL"
                     /locus_tag="IWI22_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9167631.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26880..27350
                     /gene="rpsG"
                     /locus_tag="IWI22_000207"
     CDS             26880..27350
                     /gene="rpsG"
                     /locus_tag="IWI22_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9167632.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27444..29531
                     /gene="fusA"
                     /locus_tag="IWI22_000208"
     CDS             27444..29531
                     /gene="fusA"
                     /locus_tag="IWI22_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9167633.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29703..30890
                     /gene="tuf"
                     /locus_tag="IWI22_000209"
     CDS             29703..30890
                     /gene="tuf"
                     /locus_tag="IWI22_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9167634.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31297..31605
                     /gene="rpsJ"
                     /locus_tag="IWI22_000210"
     CDS             31297..31605
                     /gene="rpsJ"
                     /locus_tag="IWI22_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9167635.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31636..32265
                     /gene="rplC"
                     /locus_tag="IWI22_000211"
     CDS             31636..32265
                     /gene="rplC"
                     /locus_tag="IWI22_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9167636.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32293..32916
                     /gene="rplD"
                     /locus_tag="IWI22_000212"
     CDS             32293..32916
                     /gene="rplD"
                     /locus_tag="IWI22_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9167637.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32916..33206
                     /gene="rplW"
                     /locus_tag="IWI22_000213"
     CDS             32916..33206
                     /gene="rplW"
                     /locus_tag="IWI22_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9167638.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33245..34078
                     /gene="rplB"
                     /locus_tag="IWI22_000214"
     CDS             33245..34078
                     /gene="rplB"
                     /locus_tag="IWI22_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9167639.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            34121..34399
                     /gene="rpsS"
                     /locus_tag="IWI22_000215"
     CDS             34121..34399
                     /gene="rpsS"
                     /locus_tag="IWI22_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9167640.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34422..34769
                     /gene="rplV"
                     /locus_tag="IWI22_000216"
     CDS             34422..34769
                     /gene="rplV"
                     /locus_tag="IWI22_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9167641.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34783..35439
                     /gene="rpsC"
                     /locus_tag="IWI22_000217"
     CDS             34783..35439
                     /gene="rpsC"
                     /locus_tag="IWI22_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9167642.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35442..35876
                     /gene="rplP"
                     /locus_tag="IWI22_000218"
     CDS             35442..35876
                     /gene="rplP"
                     /locus_tag="IWI22_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9167643.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35866..36054
                     /gene="rpmC"
                     /locus_tag="IWI22_000219"
     CDS             35866..36054
                     /gene="rpmC"
                     /locus_tag="IWI22_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9167644.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            36079..36345
                     /gene="rpsQ"
                     /locus_tag="IWI22_000220"
     CDS             36079..36345
                     /gene="rpsQ"
                     /locus_tag="IWI22_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9167645.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36403..36771
                     /gene="rplN"
                     /locus_tag="IWI22_000221"
     CDS             36403..36771
                     /gene="rplN"
                     /locus_tag="IWI22_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9167646.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36808..37116
                     /gene="rplX"
                     /locus_tag="IWI22_000222"
     CDS             36808..37116
                     /gene="rplX"
                     /locus_tag="IWI22_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9167647.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            37144..37683
                     /gene="rplE"
                     /locus_tag="IWI22_000223"
     CDS             37144..37683
                     /gene="rplE"
                     /locus_tag="IWI22_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9167648.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37702..37887
                     /locus_tag="IWI22_000224"
     CDS             37702..37887
                     /locus_tag="IWI22_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9167649.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37923..38321
                     /gene="rpsH"
                     /locus_tag="IWI22_000225"
     CDS             37923..38321
                     /gene="rpsH"
                     /locus_tag="IWI22_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9167650.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38353..38889
                     /gene="rplF"
                     /locus_tag="IWI22_000226"
     CDS             38353..38889
                     /gene="rplF"
                     /locus_tag="IWI22_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9167651.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            39050..39406
                     /gene="rplR"
                     /locus_tag="IWI22_000227"
     CDS             39050..39406
                     /gene="rplR"
                     /locus_tag="IWI22_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9167652.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39427..39927
                     /gene="rpsE"
                     /locus_tag="IWI22_000228"
     CDS             39427..39927
                     /gene="rpsE"
                     /locus_tag="IWI22_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9167653.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39942..40121
                     /gene="rpmD"
                     /locus_tag="IWI22_000229"
     CDS             39942..40121
                     /gene="rpmD"
                     /locus_tag="IWI22_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9167654.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            40165..40605
                     /gene="rplO"
                     /locus_tag="IWI22_000230"
     CDS             40165..40605
                     /gene="rplO"
                     /locus_tag="IWI22_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9167655.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40605..41900
                     /gene="secY"
                     /locus_tag="IWI22_000231"
     CDS             40605..41900
                     /gene="secY"
                     /locus_tag="IWI22_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9167656.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41962..42609
                     /locus_tag="IWI22_000232"
     CDS             41962..42609
                     /locus_tag="IWI22_000232"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9167657.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42800..43018
                     /gene="infA"
                     /locus_tag="IWI22_000233"
     CDS             42800..43018
                     /gene="infA"
                     /locus_tag="IWI22_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9167658.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            43051..43167
                     /gene="rpmJ"
                     /locus_tag="IWI22_000234"
     CDS             43051..43167
                     /gene="rpmJ"
                     /locus_tag="IWI22_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9167659.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            43185..43550
                     /gene="rpsM"
                     /locus_tag="IWI22_000235"
     CDS             43185..43550
                     /gene="rpsM"
                     /locus_tag="IWI22_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9167660.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43578..43967
                     /gene="rpsK"
                     /locus_tag="IWI22_000236"
     CDS             43578..43967
                     /gene="rpsK"
                     /locus_tag="IWI22_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9167661.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            44047..44985
                     /locus_tag="IWI22_000237"
     CDS             44047..44985
                     /locus_tag="IWI22_000237"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9167662.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            45013..45393
                     /gene="rplQ"
                     /locus_tag="IWI22_000238"
     CDS             45013..45393
                     /gene="rplQ"
                     /locus_tag="IWI22_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9167663.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45665..46504
                     /locus_tag="IWI22_000239"
     CDS             45665..46504
                     /locus_tag="IWI22_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9167664.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46480..47349
                     /locus_tag="IWI22_000240"
     CDS             46480..47349
                     /locus_tag="IWI22_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9167665.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47339..48136
                     /locus_tag="IWI22_000241"
     CDS             47339..48136
                     /locus_tag="IWI22_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9167666.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48339..49085
                     /gene="truA"
                     /locus_tag="IWI22_000242"
     CDS             48339..49085
                     /gene="truA"
                     /locus_tag="IWI22_000242"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9167667.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49418..52087)
                     /locus_tag="IWI22_000243"
     CDS             complement(49418..52087)
                     /locus_tag="IWI22_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9167668.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52325..52513)
                     /locus_tag="IWI22_000244"
     CDS             complement(52325..52513)
                     /locus_tag="IWI22_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9167669.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52783..53145
                     /locus_tag="IWI22_000245"
     CDS             52783..53145
                     /locus_tag="IWI22_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9167670.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53197..54879
                     /locus_tag="IWI22_000246"
     CDS             53197..54879
                     /locus_tag="IWI22_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9167671.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54996..57032
                     /gene="recG"
                     /locus_tag="IWI22_000247"
     CDS             54996..57032
                     /gene="recG"
                     /locus_tag="IWI22_000247"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9167672.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            57081..58082
                     /gene="plsX"
                     /locus_tag="IWI22_000248"
     CDS             57081..58082
                     /gene="plsX"
                     /locus_tag="IWI22_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9167673.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58204..58446
                     /gene="acpP"
                     /locus_tag="IWI22_000249"
     CDS             58204..58446
                     /gene="acpP"
                     /locus_tag="IWI22_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9167674.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58714..59718
                     /locus_tag="IWI22_000250"
     CDS             58714..59718
                     /locus_tag="IWI22_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167675.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59719..60663
                     /locus_tag="IWI22_000251"
     CDS             59719..60663
                     /locus_tag="IWI22_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167676.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60663..61625
                     /locus_tag="IWI22_000252"
     CDS             60663..61625
                     /locus_tag="IWI22_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167677.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61652..62566
                     /locus_tag="IWI22_000253"
     CDS             61652..62566
                     /locus_tag="IWI22_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167678.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62592..64373
                     /locus_tag="IWI22_000254"
     CDS             62592..64373
                     /locus_tag="IWI22_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9167679.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64721..65407
                     /locus_tag="IWI22_000255"
     CDS             64721..65407
                     /locus_tag="IWI22_000255"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9167680.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65427..69008
                     /gene="smc"
                     /locus_tag="IWI22_000256"
     CDS             65427..69008
                     /gene="smc"
                     /locus_tag="IWI22_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9167681.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            69017..69826
                     /locus_tag="IWI22_000257"
     CDS             69017..69826
                     /locus_tag="IWI22_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9167682.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69837..70835
                     /gene="ftsY"
                     /locus_tag="IWI22_000258"
     CDS             69837..70835
                     /gene="ftsY"
                     /locus_tag="IWI22_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9167683.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            71022..73289
                     /gene="gshAB"
                     /locus_tag="IWI22_000259"
     CDS             71022..73289
                     /gene="gshAB"
                     /locus_tag="IWI22_000259"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9167684.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73378..73830)
                     /locus_tag="IWI22_000260"
     CDS             complement(73378..73830)
                     /locus_tag="IWI22_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9167685.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            73980..74930
                     /locus_tag="IWI22_000261"
     CDS             73980..74930
                     /locus_tag="IWI22_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9167686.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74923..75882
                     /locus_tag="IWI22_000262"
     CDS             74923..75882
                     /locus_tag="IWI22_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9167687.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75879..76634
                     /locus_tag="IWI22_000263"
     CDS             75879..76634
                     /locus_tag="IWI22_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9167688.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76657..77610
                     /locus_tag="IWI22_000264"
     CDS             76657..77610
                     /locus_tag="IWI22_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9167689.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(78254..78550)
                     /locus_tag="IWI22_000265"
     CDS             complement(78254..78550)
                     /locus_tag="IWI22_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9167690.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            78905..79261
                     /locus_tag="IWI22_000266"
     CDS             78905..79261
                     /locus_tag="IWI22_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9167691.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            79271..80170
                     /locus_tag="IWI22_000267"
     CDS             79271..80170
                     /locus_tag="IWI22_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9167692.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            80687..81418
                     /locus_tag="IWI22_000268"
     CDS             80687..81418
                     /locus_tag="IWI22_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9167693.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            81563..83590
                     /locus_tag="IWI22_000269"
     CDS             81563..83590
                     /locus_tag="IWI22_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9167694.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            83698..84795
                     /locus_tag="IWI22_000270"
     CDS             83698..84795
                     /locus_tag="IWI22_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9167695.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            85102..>85549
                     /locus_tag="IWI22_000271"
     CDS             85102..>85549
                     /locus_tag="IWI22_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9167696.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        28138 a        15267 c        18128 g        24016 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg
     3841 gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct
     3901 acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg
     3961 ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt
     4021 ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca
     4081 gttatggaag ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat
     4141 tgtaaaatct gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt
     4201 cggatgctta ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta
     4261 gagccatatc ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac
     4321 gatgctccca aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt
     4381 attattggag aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt
     4441 acaaatgggc ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa
     4501 cctgtttgcc agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat
     4561 aaaataactt agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga
     4621 aatattcttt aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta
     4681 caagtggata ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac
     4741 tatacttaaa aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt
     4801 tcaaaaaatg aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt
     4861 tgagaaatga aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc
     4921 cttcaatatt tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt
     4981 tgtctgtaaa gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat
     5041 tatctgtagg taagaaaaat tcatcaatag atacatggag taatgataca agatcataca
     5101 gcacttgtgt acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa
     5161 tttcaatcat tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa
     5221 tcgctaaacc aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc
     5281 tctatacatt ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag
     5341 tttatgagtt cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata
     5401 gaaaggtcgc tgattttatt tacctataca actttataaa atttagtcat actcctagaa
     5461 agtagataaa attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca
     5521 tctagttctc ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat
     5581 gaccatcaat gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga
     5641 aaacaatctg tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc
     5701 atttatgcaa caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca
     5761 catgccactc cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa
     5821 gaaaccatag aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa
     5881 gattacttca aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat
     5941 aactaccttg tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt
     6001 aaattatttg gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat
     6061 ctttcagaga aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg
     6121 gagattgcgg agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga
     6181 ctagccatga ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg
     6241 ttcccttccc cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg
     6301 ttaaacatta tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg
     6361 gaaattctca tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct
     6421 ctaagatttt atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg
     6481 tatccacgct tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt
     6541 aggttttgat aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata
     6601 cgtttcggta agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg
     6661 attcatgtta gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg
     6721 tgataatgat actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt
     6781 acggctacat gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga
     6841 ttgctaagat attcttctct tgtcagtgta ttattataaa actatttgat ataattatag
     6901 gtgaatattg gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac
     6961 aaaaaaggat ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa
     7021 agcaaaagaa ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag
     7081 agtccctcgt gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc
     7141 tgagtagttt ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca
     7201 aagaatggaa cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt
     7261 caaaaatatc gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg
     7321 ctattgatga aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat
     7381 atctttgaaa ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat
     7441 cgcttaccaa ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt
     7501 gagcctattg atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat
     7561 gtaagagctg ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata
     7621 accaccacaa atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa
     7681 ttttggacaa aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat
     7741 caacactttt tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa
     7801 gctactgcca ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa
     7861 acactctatt acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc
     7921 gttcgtcata ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta
     7981 caacaaaatc taattcagac agactttgta atgagctata acggtattcc tacccaaaaa
     8041 catacactat catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga
     8101 atccatgggt tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt
     8161 atcattaaag atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat
     8221 ctttatccta acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa
     8281 gaagcaaaac aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga
     8341 aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac
     8401 ccttggtata agaggatttc tagctctcct acaactattc ccactccact gttgctggtg
     8461 gtttagaagt gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta
     8521 cagagatttt ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga
     8581 ttgaagttac agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac
     8641 cgacagagcg gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag
     8701 cagctgcgat ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt
     8761 cagtgatttc gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa
     8821 cgattgaatc aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg
     8881 tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt
     8941 gagatttgat cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc
     9001 cttgtgctag gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa
     9061 catatacgaa ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca
     9121 acttgtctaa gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc
     9181 ctagactttc cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga
     9241 tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa
     9301 cgccacctga aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta
     9361 cttgcatatc gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg
     9421 caaagtggcg cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga
     9481 taccataaaa attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc
     9541 cgactgtttc aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg
     9601 tttgttgttt tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg
     9661 ctttcccata ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca
     9721 attgcatacc atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga
     9781 tcccaaatgc attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg
     9841 gattcatcgc ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc
     9901 caagatcacg aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga
     9961 taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt
    10021 taaattttat ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc
    10081 gaatcgatga tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc
    10141 aagatatatt cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta
    10201 aaggcttctt cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa
    10261 gccgtatcca gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg
    10321 gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt
    10381 aattgaagcg tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac
    10441 ttgtctggaa tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga
    10501 ccagatttta cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat
    10561 cctttacggt ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca
    10621 gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt
    10681 ctggctgttg tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt
    10741 acttctacgt atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc
    10801 atataaagat aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa
    10861 atgcggtcat tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca
    10921 ttacgataaa attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc
    10981 atattagcct cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac
    11041 cccaccactt aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg
    11101 agtcttattc ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa
    11161 tgagaaggat ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg
    11221 attttgaaca atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg
    11281 gtgtattttc acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt
    11341 gggaagagtt acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg
    11401 ggttagcttt ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag
    11461 cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc
    11521 aatcgaatat ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc
    11581 cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga
    11641 agaagggcgg tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa
    11701 agaaaatgga agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata
    11761 tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac
    11821 tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa
    11881 agaactattt tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa
    11941 ataaataaga tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt
    12001 atcaaaaaac atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat
    12061 agggaattta gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa
    12121 atggcaggcg tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt
    12181 agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat
    12241 tatttcgctt caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat
    12301 gtcactgatt tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg
    12361 ggctatgtga tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta
    12421 caagagcttt cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct
    12481 gagaaatatg cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc
    12541 ggattgaatc gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa
    12601 gctgtttcgc tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact
    12661 ggatactatc aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc
    12721 ctgcctttta atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact
    12781 gcatcaaaaa aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc
    12841 atggcgatcg gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc
    12901 tcagtcatag gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca
    12961 gtgcagcagc caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc
    13021 gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag
    13081 agcgtaaaag tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca
    13141 aaggctagtt agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt
    13201 cctaaaaata atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg
    13261 atctgattga aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg
    13321 tcacgaacta tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa
    13381 ttttttatca agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt
    13441 ctggtgatgt catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag
    13501 gtggcgttgg tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga
    13561 cagtggctaa aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag
    13621 aagcaattcc cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg
    13681 aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt
    13741 atgccttgcg tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta
    13801 tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg
    13861 gtgctttcat gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg
    13921 ggaaactggc taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg
    13981 aagctatcgg aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc
    14041 cagaagatct agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca
    14101 aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag
    14161 cgttagcaaa attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac
    14221 atccagaacg aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag
    14281 ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc
    14341 gtaaaaccaa agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg
    14401 gtgacactgt gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca
    14461 cttctgtcat tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa
    14521 cgttgattca tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga
    14581 aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg
    14641 catctaaaga agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg
    14701 tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag
    14761 ccgtatgtac agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg
    14821 aaacaacaga tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg
    14881 ctttgaaatc aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag
    14941 caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa
    15001 tcatccgagc ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg
    15061 gtttctcaac aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac
    15121 cagaaatggg tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga
    15181 tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa
    15241 ctggtgaagg ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg
    15301 gatcaacaca gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt
    15361 aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc
    15421 atcatttgga ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg
    15481 agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg
    15541 tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt
    15601 aggagacaat ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac
    15661 tgaaaaagat ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt
    15721 attttaggag gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca
    15781 ctagcagcag ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt
    15841 ggcaaaagct catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac
    15901 gaccgttcat tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga
    15961 ctttcacgag gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc
    16021 ccaaatattg accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa
    16081 ctaaaaccag cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc
    16141 gatgatgtca tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct
    16201 tcttatcttg caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc
    16261 ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat
    16321 gcaggtgcga aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc
    16381 tctgctccag ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc
    16441 atcttccatg cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac
    16501 gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc
    16561 gaatacacat taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt
    16621 ggaacagtag tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa
    16681 cacactgttt atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac
    16741 gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt
    16801 ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac
    16861 aaggctatgg tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt
    16921 tggataaggg aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc
    16981 gtgaatctta gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt
    17041 tcaagaaaat attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc
    17101 aaatgacaac atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa
    17161 cggtgctggc aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg
    17221 gaaaattttg atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt
    17281 agggattggg atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa
    17341 catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa
    17401 agagatccaa aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg
    17461 tgatatttca gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc
    17521 agatgtactg atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt
    17581 aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa
    17641 gttagatgaa atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat
    17701 cggtacagtc aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag
    17761 aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat
    17821 tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga
    17881 ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt
    17941 gatccaagct ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga
    18001 agacatcact aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga
    18061 agaccgtcat aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca
    18121 aacctatcat caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga
    18181 acatgccaga gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc
    18241 aaaagcttta tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga
    18301 tcctgatctg ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt
    18361 tattcataaa cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt
    18421 cgaattagaa gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat
    18481 tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg
    18541 ctatacctta gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc
    18601 gcagcgaaaa attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct
    18661 taggcgcgat attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc
    18721 tagatgcagc ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt
    18781 tgatttttac agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc
    18841 tttctggaca agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag
    18901 atctgccaaa agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga
    18961 tggcagcggc tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa
    19021 caatcatgat gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc
    19081 ctgaaagcta tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc
    19141 ggttaccatg gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag
    19201 caatcatcgc gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa
    19261 tccgttctgt tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta
    19321 cgatcgtttt gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag
    19381 gattaggaac gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg
    19441 gaatggctgt ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct
    19501 tgttcagtat tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg
    19561 agatcgtcaa tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc
    19621 gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag
    19681 aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt
    19741 aattgccttg atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact
    19801 aggcggaact ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt
    19861 catgggtgct tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat
    19921 gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca
    19981 cgcagtagca acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct
    20041 gatggcacct gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga
    20101 taacatcacg cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat
    20161 tggaccaatt ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt
    20221 cctagcttgg gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga
    20281 aaatcctcaa gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt
    20341 actgatttct ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg
    20401 taacttttca gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt
    20461 tggtaagtgg aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag
    20521 cttgagtatc attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca
    20581 aattgctcca tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg
    20641 tccaaaagca aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg
    20701 gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa
    20761 aagaaagaag gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga
    20821 attggagaag caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac
    20881 attgcgaaag aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc
    20941 cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa
    21001 gaagtttctg tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc
    21061 aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa
    21121 gaattttctg gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac
    21181 gattacggtg aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct
    21241 gtccttcaga tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc
    21301 caattcgttc gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt
    21361 ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg
    21421 aatccattcg gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct
    21481 gttggaaaga tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa
    21541 agcaatatgg atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc
    21601 agctttacca atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc
    21661 tatgcgcatg cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa
    21721 gaagacgact tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc
    21781 gatcatacgc gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg
    21841 gcattgcctc aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt
    21901 tcttcgactg aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga
    21961 gaaagcaaat gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata
    22021 aaggagtaaa agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag
    22081 atgaaatcga agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga
    22141 cagtagttgg gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag
    22201 ccatgcaagg gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag
    22261 tacgtgtgat ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg
    22321 tcaacgaatc atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg
    22381 gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga
    22441 gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt
    22501 tggatcttaa agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg
    22561 agatccgaat gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag
    22621 tcatcgtagc agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag
    22681 cagcaggtat gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca
    22741 aacaatcttt taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat
    22801 aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg
    22861 ggagatcctt tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac
    22921 aatcaggtac gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt
    22981 caaggaacag gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg
    23041 tatggtgtaa agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat
    23101 gttcgtgatt tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat
    23161 ttccaagcct ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag
    23221 atagctaaag aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc
    23281 tacaaagatg atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa
    23341 atggaagcag cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg
    23401 atgaccgtta gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct
    23461 acgttcaatg aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat
    23521 gaaaaagctg ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt
    23581 ttgacttggc tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt
    23641 tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg
    23701 tttgatgaat attgtgactt gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa
    23761 aaagggactg tgacaaatcg tgtcacagtc ccttttttat agtgtttttt ataggtagta
    23821 ttttttagca actgttaagt catcatttaa ttcataaaca agtggttgac ctgttggaat
    23881 ttcaagatcc atgatgtctt cgtcagagat accttcgata tgtttagcta gggcgcgtaa
    23941 tgagttacca tgtgcagcta ctaaaacagt tttgttgtct agtaatgctg gagcaatttc
    24001 atcttgccag aatggcaaag cgcgttctaa tgtcactttc aagttttctc caccaggaat
    24061 atcgcgtttg tctaacattg cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc
    24121 ttccattaat ggaggaagag tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc
    24181 gtatttttca gctgtttctt ttttgttcaa accttgtaat ttaccatagt gacgttcgtt
    24241 taagcgccat gatttgattt gtggtaccca aagttgatct gaatattcta agatcaagtt
    24301 acatgttttg attgcacgag ttaaaacaga agtgtaagca acatcaaatt cgattccagc
    24361 ttctttgatt ttgcgtccgc cttcttttgc ttcttcgatt ccttctggtg ctagatttac
    24421 atcagcccaa ccagtaaatt ggttcaatgc attccattca ctaagaccgt gacgagaaaa
    24481 aactaatttt ggcatgttta gctctccttt taagataggt ttatatttct gaaattttca
    24541 gttctgtttt attgtacaat tttttggaag agatttccat cgaaaaatta gataagattc
    24601 tatgagaaaa caagtagaga atataaaaaa acgaacagtg cccacgcaac tgttcgtttt
    24661 taggtattag atgcataatt gctcgatttc tttttcaggt aaattgatat atttataaca
    24721 tgttccaact gaaacaccgc attttgaaga aataaattgg atcgtttctt ttttttcata
    24781 atagagacgt cggatttttt tgatcgtttt tgcatcgatt tttggtcttc cgccgatttt
    24841 tcctttttta cgagcttcat ctaatccaac taatgtacgt tctttgatca atgcgcactc
    24901 catgtttgct aggttttcca ttaattggaa atagattttt cccatttcgc gcgtatcgat
    24961 tccttcatca agactaatca agtgcagccc gcgatctttg aataattgcg tgagttctgt
    25021 taattgccgc gtagatttgc ctaatcgatg aagttggcaa atgactagtg catcgccttg
    25081 ttgcatgtca gcgatcactg actccaaatg aacagcagta gaatcatttt gttccacttc
    25141 atatgtctca tggaaaattt cttgacaacc aaagtcagat aattgatcaa cctgagttgt
    25201 taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata attttcatgg tgattccttt
    25261 ctagcagtga ttttctaact ggatataaca gtaaaaatgt agcataaagt acgtcaaaaa
    25321 gatagttttt tcgcaaattt tccgctgtct ttttttattg gatgatcaag cagtttttct
    25381 ttatttacga cggattgcgt caagaatctc aacgcctttt tctgagccga caactacttt
    25441 agaaaccatg tttaggaaaa gcccatgttc tactacccca acaagttcat ctaaataggt
    25501 agctaagctt tctggctgtt cgatgacttc tagatgcaga tcaatgatgt aatgaccacc
    25561 atctgtggtt aagacttctc cgttttcgtc tgtacgcaag acgggttgga atcttttttc
    25621 ttcaaaaata tgaagtagct gttgggagcc ataagggatc acttcaactg ggagtgggaa
    25681 cttcccaagt ttatcgacta atttagaaga atcaacgatc caaatcgttt cttttgagta
    25741 cgtagcgact attttctcga aaagcaaagc agcgccgccg cctttgatcc cttgaaaatc
    25801 agcactgatt tcatcagcac cgtctattgt caaatcgact acggggactt catcaatact
    25861 ctttaacggt attcctaatt tttccgcttg ttcttttgtc gcatttgaag tagtaactcc
    25921 tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc tcttctacca tataataggc
    25981 ggttgaaccg gtcccgagac ctacgatcat cccgtccttt acgaatttcg cggcttcaat
    26041 gccagctaat tgttttaaat tcatctttgc gtccaccttt ttttagtttg agcatcgtgc
    26101 tttcctcact attttagcgt gatttacggt aaaaagatac cattcttttt tattccaata
    26161 aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt ttttcattcg tgatattcta
    26221 acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg ctgactgttt atcaaagcat
    26281 atttgataag gatgcagaca ttgcatttat tttttatcac aaaaaaagaa ccacctggat
    26341 gtgtggaact agacgcaaat cgtgggaagg aggaaacaag gaatgcctac aattaaccaa
    26401 ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta attcacctgc tttgaacaaa
    26461 ggatataaca gctttaagaa aactcaaact aacgtaaact cacctcaaaa acgtggggta
    26521 gcaactcgtg tgggtacaat gacacctaaa aaaccgaact cagctttacg taaatatgca
    26581 cgtgttcgtt tgtcaaactt aattgaagtt actgcttata tcccagggat tggtcataac
    26641 ttacaagaac acagtgttgt attattacgc ggtggtcgtg taaaagactt accaggggta
    26701 cgttaccata tcgttcgcgg tgctttagat actgctggtg ttaacgaccg taaacaaagc
    26761 cgttctaaat acggaactaa aaaacctaaa gcttaatttt aattattaaa caaatgaaac
    26821 ttgcttctac tatatagaag taattactat gaacaatatt tcggaaggag gagttacgga
    26881 tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc agatcctatt tataactcaa
    26941 aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg aaaacgcggg attgcagcta
    27001 acattatcta taatgccttt gacttgatca aagaatctac aggtaacgat ccattagaag
    27061 tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga agttaaagca cgtcgtgttg
    27121 ggggttcaaa ctaccaagta ccagttgaag ttcgccctga acgccgcgtt actctaggtc
    27181 ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca cacaatggaa gaacgcttag
    27241 caaaagaaat catggatgct gcaaacaaca caggtgcttc tgttaaaaaa cgtgaagaca
    27301 ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta tcgctggtaa gattctctct
    27361 gtttggtcgg cttttataag tcgacctcag atagtgtcct tatttttagc aactagatag
    27421 taaagagagg agaaacaagg agaatgacca gagaattttc tttacaaaac actcgtaata
    27481 tcggtatcat ggcccacatc gatgctggta aaacgactgc tactgaacgt atcttgtact
    27541 atactggtaa aatccataaa attggtgaaa ctcatgaagg agcttcacaa atggactgga
    27601 tggaacaaga acaagaacgt ggtattacga tcacttctgc tgctactaca gcacaatgga
    27661 agggtcaccg aatcaacatc atcgacacac caggacacgt agacttcaca gttgaagttg
    27721 aacgttcatt gcgtgtactt gatggtgctg taactgttct tgatgcacaa tcaggtgttg
    27781 aacctcaaac tgaaacagtt tggcgtcaag caacaactta tggtgtacct cgtatcgtat
    27841 ttgcaaacaa aatggacaag atcggtgctg acttcttata ttcagtatca actttacatg
    27901 accgtttgca agcaaacgct cacccaatcc aattaccaat tggtgcggaa gacgacttca
    27961 caggaattat cgaccttgta acaatgaagg ctgaaatgta tacaaacgat cttggaacag
    28021 agatcgaaga aacagaaatt cctgaagaat accgcgaatt agctgaagaa tggcgcgaaa
    28081 aattggttga agctgtagct gaaactgatg aagaacttac tttgaaatac ttagaaggtg
    28141 aagaaatcac tgaagctgag ttaaaagaag gtatccgccg cgcaacagtt aacgtagaat
    28201 tctacccagt actatgtggt tcagcattta agaacaaagg ggttcaatta ctattggacg
    28261 ctgttcttga ttacttgcca tcaccgttag atatcccagc tatcaaaggg attgacccta
    28321 aaactgatga agaagtagaa cgtcctgcag acgacagcgc tcctttctca gctttagcat
    28381 ttaaagttat gactgaccca ttcgtaggtc gtttaacttt cttccgtgtg tactctggtg
    28441 ttttacaatc aggttcatac gtacaaaacg caacaaaagg caaacgtgaa cgtgttggtc
    28501 gtatcctaca gatgcatgcc aactctcgtt cagaaatctc tgaagtttac gctggagata
    28561 tcgctgctgc tgttggtttg aaagacacaa ctactgggga cacactatgt gatgaaaaga
    28621 gtctagttat tcttgaatcc atggaattcc cagaaccagt tatccaagtc gctattgaac
    28681 ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt acaaaaacta tctgaagaag
    28741 atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga aacaatcatc gctggtatgg
    28801 gtgaacttca cttagatatc atcgttgacc gtatgcgtcg tgaattcaaa gttgaagcta
    28861 acgtaggtgc gcctcaagta tcttaccgtg aaactttccg tgctggcact caagccgaag
    28921 gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg tcacgtatgg attgaattta
    28981 caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc gatcgtcggt ggggttgttc
    29041 ctcgtgaata cattccagct gttgaaactg gtttgaaaga tgctatggaa aatggtgtct
    29101 tagctggcta tccattagta gatatcaaag ctaaacttta cgatggttca taccatgatg
    29161 tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat ggcattacgt gcagcagcta
    29221 agaaagctaa tccagtaatc cttgaaccaa tcatggctgt agaagttgtt atccctgaag
    29281 attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg tggacgtgtt gaaggtatgg
    29341 aagctcgcgc tggcggccaa caagttgttc gtgcgatggt tccacttgct gaaatgtttg
    29401 gttatgctac aactcttcgt tctgctactc aaggtcgtgg tacatttaca atgacatttg
    29461 accactacga agatgtacca aaatcagtac aagaagaaat cattaagaaa aacggcggaa
    29521 aagctgaata atttcttaaa accggatagt cctgcaaaga gattatccaa agagactata
    29581 tttttaatat agtttcgtgt aaaataatta tcgatgatat gggcatgtgg tgtaccttgt
    29641 gtacatcata tacctatcag acaaataaaa aatatattgt atttttagga ggacatttta
    29701 aaatggcaaa agaaaaattt gaccgttcta aaccacacgt taacattggt actatcggac
    29761 acgttgacca tggtaaaact acattaacag ctgcaatcac aactgtacta tctaagaaaa
    29821 acggcggcca agctatggct tacgatcaaa tcgatggtgc tccagaagaa cgcgaacgcg
    29881 gaatcacaat ctcaacagct cacgttgagt atgaaacaga tacacgccac tacgctcacg
    29941 ttgactgccc aggacacgcg gattacgtta aaaacatgat cactggtgct gctcaaatgg
    30001 acggagctat cttggtagtt tctgctgctg acggcccaat gcctcaaact cgtgaacaca
    30061 tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt attcttgaac aaagtagaca
    30121 tggttgatga cgaagaatta ctagaattag ttgaaatgga agttcgtgac ctattaacag
    30181 aatacgaatt ccctggtgac gatgttcctg tagttgctgg atcagctttg aaagctctag
    30241 aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat ggctgcagtt gacgaataca
    30301 tcccaactcc agaacgtgac aacgacaaac cattcatgat gccagttgaa gacgtgttct
    30361 caattactgg acgtggtact gttgctacag gtcgtgttga acgtggacaa gttcgcgttg
    30421 gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc aaaaacaaca gttactggtg
    30481 ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg agacaacatt ggtgctttac
    30541 tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt tttagctaaa ccaggtacaa
    30601 tcacacctca tacaaaattc tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac
    30661 gtcatactcc attcttcact aactaccgtc cacaattcta cttccgtaca actgacgtaa
    30721 caggtgttgt tgaattacca gaaggaactg aaatggttat gcctggcgac aacgtaacaa
    30781 tggaagttga attaatccac ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg
    30841 aaggcggacg tacagttggt gccggtgtcg ttacagaaat caaagcttaa ttcacttaag
    30901 ttttaaagta aagcaacatt tattcggacg taatcgctcg gaacgaaggt cttaggatct
    30961 ttgttccgag cttttttatt tattgcagga tcaggaaaaa agacaggagt attataaata
    31021 gaagaaacac gaaataaaag agtcagtatt ttctaaatga aactttatga aaacaaaatg
    31081 ttttttacaa gaaaaagcct tgctttgtga gtttatatat attataatga caaaggtgcg
    31141 tttactttag tgaattgcac agacgtaatc agaatgattt cgtcggctaa gaaacgagag
    31201 gttgcgacac gcccggacgc tttgccacga acgagcgtga cggaaatttt cgtggagcta
    31261 tgtctacttt tcaaaatagg cgaaggaggg aacaagatgg caaaacaaaa aattcgtatc
    31321 cgtttaaaag cgtatgaaca ccgtatttta gatcaatcag cggataaaat cgtggaaact
    31381 gcaaaaagaa ctggagctag cgtgtctggt ccaattccat taccaactga acgcagtctt
    31441 tacacaatca tccgtgcgac tcataaatac aaagattcac gcgaacaatt cgaaatgcgt
    31501 acacacaaac gtcttatcga cattgtgaac ccaacaccaa aaactgttga tgctttgatg
    31561 aagctagact taccatctgg tgtaaacatc gaaatcaaat tataaaacta aaacaaaaat
    31621 aatggaggtg tactcatgac caaaggaatc ttagggaaaa aagtgggaat gacacaaatc
    31681 ttcactgaat ctggtgaatt aattccagta actgttgtag aagctactcc aaacgtagta
    31741 ctacaagtga aaacaatgga aacggatggc tacgaagcta ttcaagttgg ttaccaagat
    31801 atgcgtgaag ttttatcaaa caaacctgcg aaaggtcatg ttgcaaaagc aaacacggct
    31861 cctaagcgct tcattcgtga attcaaaaat gttgagctag gagaatatga agtaggaaaa
    31921 gaaattacag tagatgtttt ccaagcagga gacattattg atgtaacagg tactacgaaa
    31981 ggtaaaggat tccaaggggt tatcaaacgc cacggacaaa gccgcggacc aatggctcac
    32041 ggttctcgtt accatcgtcg tcctgggtca atgggtccag ttgcacctaa ccgtgtattt
    32101 aaaaacaaac gtcttgctgg ccgtatgggt ggcaaccgcg taactatcca aaacttggaa
    32161 gttgtacgtg tcgatgcaga aagaaatgtt atcttgatca aaggtaatgt tcctggagct
    32221 aaaaaatctt taattactat caaatcagct gtgaaagcta aataattgta ggaagaaagg
    32281 aggaactaag gaatgccgaa tgtagcatta ttcaaacaag atggaagcca aaacggtgaa
    32341 atcacactta acgaagaaat cttcggaatt gaacctaatg aaagtgttgt ttacgatgca
    32401 atcgtaatgc aacgtgcgtc attaagacaa ggaacacacg cggttaaaaa ccgtagcgct
    32461 gttcgcggcg gcggacgtaa accatggcgt caaaaaggaa caggtcgtgc acgtcaagga
    32521 tctatccgct caccacaatg gcgtggaggt ggcgtagtct ttggaccaac accacgttct
    32581 tacagctaca aacttcctaa aaaagttcgt cgtttagcaa tgaaatctgt attgtcagaa
    32641 aaagttgctg aaaacaactt ggtagcaatc gaaggcttga acttcgacgc accaaaaaca
    32701 aaagaattca aacaagttct tgctaactta tctatcgact ctaaagtatt ggttgttcta
    32761 gaaaaaggta acgactttgc tgctttatca gcacgtaact taccaaacgt ttctgtagta
    32821 acttctgata atgtgagtgt gctagacgtt gtatcaaata caaaagtgtt agcaacacaa
    32881 actgctctta ctcaaattga ggaggtgctt gcataatgaa cttactagac gtaatcaaac
    32941 gcccagtgat cactgaaaaa tcaatgcttg ccatggatga caagaaatac acttttgaag
    33001 tggacactcg cgcaaacaaa actttagtaa aacaagcggt agaagctgct tttgacgtta
    33061 aagtcaaaaa cgtgaacatc gtgaacgtac gtgcgaaatt caaacgtatg ggcaaatatg
    33121 caggatatac aaaaaaacgt cgcaaagcga tcgttacatt gactgaagat tcaaaagaaa
    33181 tccaattatt cgaagctgct gaataagcac taatagataa ctcattaaat taggagggaa
    33241 aaacgtggcg attaaaaagt acaaacctac ctcaaatggt cgtcgtaaca tgacaggttc
    33301 tgattttgct gaaatcacaa catcaacacc tgaaaaatca ttgttacaac cattgaaaaa
    33361 ccatgctgga cgtaacaaca acggccgcat cactgtacgt catcaaggtg gcggacacaa
    33421 acgccaatat cgcgtgatcg acttcaaacg taacaaagat aacgttgttg caactgttca
    33481 aacgatcgaa tatgatccaa accgttcagc taacatcgcg ttagtacact atgaagatgg
    33541 agtgaaagct tacatcttag cacctaaagg actagaagtg ggtatgaaac ttgtttcagg
    33601 tccagaagca gatatcaaag tcggaaatgc tcttccatta gaaaacattc cagttggtac
    33661 tgttatccac aacattgaaa tgaaacctgg taaaggtgga caattgatcc gttctgctgg
    33721 tacaagtgct caagtacttg gtaaagaagg caaatacgta ttgatccgct tgaactctgg
    33781 tgaagttcgt atgatcttag ctgcatgccg tgctacaatc ggttctgtag gtaacgaaca
    33841 acacgaatta atcaacatcg gtaaagctgg acgctctcgt tggatgcgta aacgcccaac
    33901 tgtacgtggt agcgtaatga accctaatga tcacccacac ggtggtggtg aaggtaaagc
    33961 tcctatcgga cgtaaagcac cagtttctcc ttggggtcaa ccagctcttg gttacaaaac
    34021 tcgtaacaaa aaagctaaat cagacaaact tattgttcgt cgtcgcaaaa ataaataatt
    34081 actttgacca tgtgtcgcac attttgagag gaggttcacc atgggtcgta gtttaaagaa
    34141 aggacctttt gtcgatgagc atttaatgaa aaaagttgaa gctcaagcag gggttgaaaa
    34201 gaaaaaagtt atcaaaactt ggtcacgccg ttcaacagtt ttcccaagtt ttgttggata
    34261 taccatcgca gtttatgatg gacgtaaaca tgtaccagtt tacatccaag aagacatggt
    34321 aggacacaaa ttaggtgaat ttgcacctac aagaacttat cgtggacatg ctgctgacga
    34381 taagaaaacg aaacgctaat ttgaggggag gataaatcca aatggcagaa caaatcacat
    34441 cagctaaagc aactgcaaaa acagttcgca cttcacctcg aaaagcccgt ttagtaattg
    34501 accttatcag aggtaaaagt gtcgcagatg caatttcaat cttgaaattc acaccaaaca
    34561 aatctgctgg aatcattgaa aaagtattga tgtcagcagt tgcgaatgca gaaaataact
    34621 ttgacttaga tgttgaaaac ttggtagtaa gcgaagcttt tgttaacgaa ggacctacaa
    34681 tgaaacgttt ccgtcctcgt gcaaaaggat cagcttctcc aatcaacaaa cgtacaagtc
    34741 atatcacggt agtcgtatca gaaaaataag gagggataat cagtgggtca aaaagtacat
    34801 ccaattggaa tgcgtgtagg catcatccgc gactgggatg ctaaatggta tgctgaaaaa
    34861 gagtatgctg agttcttaca cgaagattta agaatccgta aatttatttc aactaaactt
    34921 gctgatgctg ctgtatcaac agttgaaatc gaacgcgcaa caggtcgcgt aaacatttca
    34981 attcacacag ctaaaccagg tatggttatt ggtaaaggcg gttctgaagt cgaaagccta
    35041 agaaaagaat taaacaaatt aactggtaaa agaatccata tcaatatcgt ggaaatcaaa
    35101 aaaccagatc tagatgcaaa attagtaggt gaaggaatcg cacgtcaatt agaaaaccgt
    35161 gttgctttcc gtcgcgctca aaaacaagcc atccaacgct caatgcgtgc tggcgctcaa
    35221 gggatcaaaa ctcaagtatc tggtcgtcta aacggagcag atatcgctcg ttcagaagga
    35281 tactcagaag gaactgttcc tcttcatact ttacgtgcgg acattgatta cgcatgggaa
    35341 gaagcagata caacatacgg aaaactagga gttaaagtgt ggatctatcg tggagaaatt
    35401 cttccaacta aaaaaaacac tgagaaagga gggaaataat catgttagta cctaaacgtg
    35461 taaaacaccg tcgtgaattc cgcggaaaaa tgcgcgggga agctaaaggc ggaaaagaag
    35521 tagcattcgg tgaatacggt ttgcaagctg ttgattcaca ttggatcaca aaccgccaaa
    35581 tcgaagctgc tcgtatcgca atgactcgtt acatgaaacg tggtgggaaa gtatggatta
    35641 aaattttccc tcacaaatct tatactgcca aagcaattgg ggtacgtatg ggttctggta
    35701 aaggggcacc tgaaggatgg gttgcaccag taaaacgtgg taaaatcatg tttgaaatcg
    35761 caggcgttcc tgaagaagta gctcgtgaag cgttacgtct agcttctcac aaattaccaa
    35821 tgaaaactaa gatcgtaaaa cgtgaggaaa tgggtggtga atcgaatgaa ggttaaagaa
    35881 atcagagaat taaccactgc cgaaatgctt gatcaagaaa aacaattaaa agaagaattg
    35941 tttaatctta gattccaatt agcaacaggt caattagaaa acactgcacg tattaaagaa
    36001 gtacgtaaat cgattgcacg catcaaaact gtgttgcgtg aacaagctaa gtaattatgg
    36061 aaggaggcca ttaagcgtat gactgaagga agaaatcaac gcaaagttta tcaaggtcgc
    36121 gtggtttcag acaaaatgga taaaacaatt accgttgctg tcgaaacaaa gaaaaaccac
    36181 cctatctatg gcaaacgcat gaactattct aagaaataca aagtgcatga tgaaaacaac
    36241 acagcaaaag ttggcgacat cgtgagaatt atggaaactc gtccattatc agctacaaaa
    36301 cgtttccgtt tgttggaagt agtcgaagaa gcagttatta tctaataaca cgagattttc
    36361 ctatatagat tgaatgcaaa atctgaaagg aggatacaca acgtgatcca agcagaaagt
    36421 cgtttaaaag tcgctgataa ctcaggcgca cgtgaaattt tgacgatcaa agtccttggt
    36481 ggatctggtc gtaaaactgc taacatcggt gatgtgatcg ttgctacggt taaacaagca
    36541 acgccaggtg gggttgtcaa aaaaggtgac gtcgttaaag ccgttatcgt tcgaacaaaa
    36601 tcaggagctc gtcgtacaga cggttcatat attaaatttg atgaaaatgc tgcagtgatt
    36661 attcgtgatg ataaaagccc tcgtggaaca cgtatctttg ggccagttgc acgtgaacta
    36721 cgtgaaaaca acttcatgaa gatcgtttca ctagcaccag aagttttata atcttgagac
    36781 acttatcaaa ggaggtgcga aacagttatg tttgttaaaa aaggcgataa agttaaagtt
    36841 atcactggaa aagacaaaaa caaagaaggc gttgtactag cagcgttccc gaaaaaagat
    36901 aaagttattg tcgaaggtgt aaatgtcgtt aaaaaacacc aaaaaccttc tcaagctgct
    36961 cctcaaggtg gaattgttga gatggaagcg ccaattcatg tttctaacgt gatggtggtt
    37021 gactctactg gtgtagctgg ccgagttggc tacaaagaag tagacggcaa aaaagtccgt
    37081 gtttctaaaa aaaccggtga agttttagat aaataaaagt ttaaggaagg agggtcttac
    37141 tgaatgaacc gcctaaaaga aaaatatctt aaagaagtaa ctccatcatt gatggaaaaa
    37201 tttaactata gctctgttat gcaaacacct aaagttgaaa aaatcgtcat caacatgggt
    37261 gttggtgatg cggtatcaaa cgctaaaaac ttagataaag cagttgaaga attaactttg
    37321 atcactggtc aaaaaccaat gattacaaaa gctaaaaaat caatcgctgg attccgttta
    37381 cgtgaaggaa tgccaattgg tgcaaaagtt acattgcgcg gagaaagaat gtacgaattc
    37441 ttagacaaat tggtttctgt ttcactacca cgtgtacgtg acttccatgg tgtaagtaaa
    37501 aaagcctttg acggtcgtgg aaactatact ttaggtatta aagaacaatt gatcttccca
    37561 gaagtagatt atgatttagt agataaagta cgtggtatgg acatcgttat tgtaacaaca
    37621 gcaaactctg atgaagaatc tcgtgagttg ttgacacaat taggcatgcc attccaaaaa
    37681 taattaaagg aggcgaacta cgtggctaaa aaatcaatga ttgctaaaaa caaacgtcct
    37741 gcaaaatttt caacacaagc ttatactcgt tgcgaacgtt gcggacgtcc acattcagtt
    37801 tatcgtaagt tccatctttg ccgtatttgc ttccgcgaac ttgcctataa aggtcaaatt
    37861 cccggcgtga agaaagctag ctggtaaaaa gtaaattcgc ataagaagga ggtaaaagat
    37921 caatggtcat gacagatcca attgcagatt ttctaactcg tatccgtaac gcaaacatgg
    37981 ttaaacatga gtctttagaa gtacctgcat caaaaattaa acgtgacatc gctgaaatct
    38041 tgaaacgtga aggtttcgtt cgtgatgttg aatacatcga agatgacaaa caaggcgtga
    38101 tccgtgtttt cctaaaattt ggtaaaaacg gcgaacgtgt tatcacaaac ttaaaacgta
    38161 tctctaaacc aggtttgcgt gcttatgtca aagctgacga agtaccaaaa gtattgaacg
    38221 gtttaggtat cgcaatcatt tcgacttctg aaggtgttgt cactgataaa gaagctcgtg
    38281 aaaaaaatat cggcggcgaa gttatcgctt acgtatggta atcaagtaga atctacaagg
    38341 aggtgtcttt aagtgagccg tatcggaaat aaagtcgttg ttatccctga aggagtaaca
    38401 gttactcaag acggaaacaa cgttacagtt aaaggaccaa aaggtgaatt gactcgtact
    38461 ttttcagcag atatcaaaat gaatatcgaa ggaaacgaag taactttcac tcgtccaaac
    38521 gacagtaaag aaatgaaaac aattcatgga acaactcgtg cgaacttcaa caacatggtt
    38581 gtaggtgtca gcgaaggttt ccaaaaagcg ttagaactaa tcggggttgg gtaccgtgca
    38641 caattacaag gatcaaaact tgtattgaac gttggttact cacatccagt agaaatcaca
    38701 ccaccagctg gagtaactgt tgaagtacca gctaacacac aagtaattgt taaaggtgcg
    38761 aacaaagaag aagttggcga attagctgca aacattcgtg gaactcgtcc tccagaacct
    38821 tataaaggta aaggtattcg ttatgttggc gaatatgtac gccgtaaaga aggtaaaact
    38881 ggtaaataat cgtttagaaa aattgcatag aagccagcag caggaaaaag gcatttggct
    38941 gaagtttacc ttcaaaaaaa tacattttta cttgtgccgc agcttctatt tttcgaaacg
    39001 tatttactgc ttgaaatagt agtacaataa aaataaagag gtgacaattg tgattacaaa
    39061 accagataag aacaaaacac gtcaaaagag acatcgtcgt gtacgtaaca atatctctgg
    39121 tactgctgag cgcccacgct tgaacgtttt tcgttctaac aaaaacatct acgctcaaat
    39181 tattgatgac gtagcgggtg taacgctagc aagtgcctct accttggata aagaaatttc
    39241 aggtggaaca aaaacagaag cagcacaagc tgtcggtaaa ctagttgctg aacgtgcagc
    39301 tgaaaaaggc attaaagtag tagtctttga ccgtggtgga tacctttacc atggccgtgt
    39361 gcaagcttta gctgaagctg ctcgcgaaaa tggactagaa ttttagaaga aggaggaata
    39421 ccattcatgg tttatatcga tccaaaacat ttggaattag aagaccgcgt tgttgcaatt
    39481 aaccgtgtaa caaaagttgt taaaggtgga cgtcgtctac gttttgctgc tttagttgtt
    39541 gtcggtgaca aaaacggaca cgtagggttt ggtacaggta aagctcaaga agttcctgaa
    39601 gcaatccgta aagcaattga agatgcgaag aaaaacttag ttgaagtacc tatggttggt
    39661 tcaacaatcc cacacgaagt aatcggtgct tttagtggtg gtagaatcct aatgaaacct
    39721 gctgtagaag gttctggggt tgctgctggt ggaccagttc gtgccgtatt agaattagct
    39781 ggggtagcag atatcacttc taaatcttta ggctcaaata caccaatcaa cgttgttcgc
    39841 gcaacagttg aaggtttgaa acaattgaaa cgtgcagaag aagttgcaga acttcgcggc
    39901 aaatcagttg aagaattaat cggataagga ggacaacaat aatggctgaa ttaaaagtta
    39961 ctttaaaacg cagtattatc ggacgtcctc aaaaccaacg cgatacagtt aaagcgttag
    40021 gtcttacaaa gattaacagt tcagtggtta agccttctaa tgaagcaatt aaaggcatga
    40081 tcaatactgt ttctcaccta gtggacgttg aagaaattta atttagaaaa atcataacta
    40141 gaacattaag gaggtgccaa acctatgaaa cttcatgaat taaaacctgc tgaaggttca
    40201 cgccaagtac gtaaccgtgt tggtcgtggt acttcatctg gtaacggaaa aactgccgga
    40261 cgcggacaaa aaggacaaaa agctcgttca ggcggcggtg tgcgtttagg atttgaaggg
    40321 ggacaaacac cattgttccg tcgtttacca aaacgaggat tcacaaacgt gaaccgtaaa
    40381 gactatgcag tcattaatct tgatgtattg aaccgctttg aagacggagc tgaagtaact
    40441 cctgttgctt tagttgaagc tggaatcgtg aaaaacgaaa aagctggaat caaagtatta
    40501 gctaatgggg aactaaacaa gaaattaact gtgaaagcag caaaattctc taaagcagct
    40561 caagaagcaa ttgaagcggc tggtggatca atcgaggtga tctaatgttt agactattaa
    40621 aagatgcttt taaagtaaaa gacattagat cgaaaatcct atttactgta tttgtgttgt
    40681 ttgtatttcg cctaggtgca catattactg taccaggcgt aaatgctgct tcattgcaaa
    40741 acctttcttc tttgccattt ttcaacatgt tgaacttggt aagtggtagt gcgatgcaaa
    40801 acttttccat tttctcaatg ggggtttcac catacattac tgcttctatc gtcattcagc
    40861 ttcttcaaat ggacatcgtg cctcgatttg ttgaatggtc gaaacaaggg gaagttggtc
    40921 gtaaaaaact gaatcaagcg actcgatatc tgacattagt attggcattt atccaatctg
    40981 tcggtatcac tgctggattc aatcaatacg ctcaattagg tttcgtgaat aatcctaacg
    41041 tagcaactta cgtaacgatc ggtttgattc taacagccgg tacgatgttt gttacttggc
    41101 taggagaaca gatcacagaa aaaggtatcg gaaatggcgt atcaatgatc atttttgcag
    41161 gtatcatttc tagattgcct gaaagtatca gagaaattta tgaagattac tttataaata
    41221 ttgatccttc agacatatgg aaatctgcta tttttgtggc cattttacta gtagcggtct
    41281 tgttgatcgt gacattcgta acatttttcc aacaagctga acgcaagatc ccgatccaat
    41341 atacgaaacg cgtagctggc gcgcctacaa gcagctatct gccgttgaaa gtaaatgcag
    41401 ctggagttat tccagttatc tttgctagtt cgtttattgc tacgccaaat gctgttttac
    41461 aagcattgag cggtagattc ggttctgaaa actggtacaa agtgatgatg gagatcttta
    41521 actataatac agcacctggt gctacgattt atacgattct gatcgttgcc ttcactttct
    41581 tctacgcctt tgttcaagta aaccctgaga aattagcgga aaacttgcaa aagcaaggaa
    41641 gttatatacc aagcgtacgc cctggtaagg ggactgaaga gtatgtctca agattactga
    41701 tgcgtttaag tacagttggt gcagtcttcc ttggtttagt ggcattgctt ccaatcattg
    41761 ctcaaatggt ttgggatctt ccacaatcaa tcggattagg cggtacaagc ttactgatcg
    41821 ttatcggtgt agctttagaa actgcgaaac agttagaagg cttaatgctg aaacgtaaat
    41881 atgttggatt cattgagtaa gaaagcgtgt tgaggtttcc cctcaacaca cgttctcaca
    41941 catttattag gagggaaaac aatgaacctc attttaatgg ggctgccagg cgcaggtaaa
    42001 ggaacacaag ctgaaaaaat cattgatgct tatggtattc cgcacatctc aacaggagat
    42061 atgttccgcg cagcgatgca aaacgaaaca gctcttggcc tggaagcaaa gtcttatatg
    42121 gataaaggtg cattagttcc tgatgaagta acaaatggaa tcgtgaaaga gcgactagct
    42181 gaaccagata cagagaaagg cttcttatta gatggctttc cacgtacgct agatcaagca
    42241 aaagctctgg acgcaatgct aaaagatttg aataaaaaaa ttgatgctgt catcgatatc
    42301 catgttggtg aagaaatctt ggtcgaacgt ctagccggcc gatttatctg cagcaattgt
    42361 ggagcaactt accataaaat tttcaaccct acaaaagttg aagacacatg tgatcgttgc
    42421 ggtgggcatg aattctatca aagagaagat gataaacctg agacggttaa aaatcgtctg
    42481 gctatcaaca tcaaaaatag tgaaccaatc ttggcttatt ataaagaaca aggtttgcta
    42541 aacactattg atggtgctcg tgaaatcgat gctgtatttg cagatgtaca aaaaatcatt
    42601 gaaaaatagc acgattgccc gtagccgact tgcattatgc agttactctt gtcaagttca
    42661 ataaattatg atagaatatg tcagtccgac ttctagagaa tcctctagat gatccatttt
    42721 gagaattttg aggtgggaac tgcagtttcc gccattttat cgtgtgaaaa tgcgaaacaa
    42781 gtacaaggag gtactgtgcg tggcaaaaga agatatgatt gaagtcgaag gtacagtcgt
    42841 cgaaactttg ccgaatgcaa tgtttaaagt tgaactagaa aacggacacc aagttcttgc
    42901 tactgtttca ggtaaaattc gtatgcatta catccgtatc ttacctggtg acaaagtaac
    42961 ggttgaattg tctccatacg atcttaaccg tggccgtatt acttatcgct ttaaataatt
    43021 gtactccgta aaactacagg aggtattatc atgaaagtaa gaccatcagt aaaaccaatg
    43081 tgcgaacatt gtaaagtaat tcgtcgtaaa ggacgcgtta tggtgatttg cccagcaaat
    43141 ccaaaacata aacaacgtca aggataattg gaggtgctat aaatatggct cgtattgcag
    43201 gagtagatat tcctcgtgat aaacgtgtag tagtttcact tacttatatc tatggtattg
    43261 gtaacactac tgctaaaaag atcttagctg atgctggtgt atctgaagat gttcgcgttc
    43321 gtgaattaac gaatgaacaa acagatgcta tccgtgcaga agtagataaa ttaaaagttg
    43381 aaggggatct tcgtcgtgaa gtgaacttga acatcaaacg tttgatggaa atcggttctt
    43441 accgtggaat ccgtcatcgc cgtggattgc ctgttcgtgg acaaaacacg aaaaacaatg
    43501 cacgtactcg taaaggccca gctcgcgcta tcgctggcaa gaaaaaataa ttcttaagtg
    43561 aaggaggtta aaacttcatg gcagcaaaaa aagtgaatcg taaacgccgt gtgaagaaga
    43621 atatagaagc aggtattgca catatccatt ctacattcaa taacactatc atcatgatca
    43681 ctgatactca cggaaatgca ttagcgtggt catcagctgg ttcattaggt ttcaaaggaa
    43741 gcaaaaaatc aactcctttt gccgctcaaa tggcagcaga aactgctaca aaagcagcaa
    43801 tggaacacgg attaaagact gttgaagtaa cagttaaagg acctggttct ggacgtgaag
    43861 cagcaattcg ttcattacaa gctacaggtt tagaagtgac tgcaattcgt gacgtgactc
    43921 cagttcctca taatggtgcc cgccctccaa aacgccgtcg tgtttaatga gtgccgctca
    43981 ttttgagttt tatcagtaac gtcattgaac aagacacttt tcgttttgaa aggggtaaag
    44041 ataagaatga ttgagttcga aaaaccaaga atcgcaaaaa ttgatgaaga aaaagattat
    44101 ggcaagttca tcgttgaacc tcttgaaaga gggtatggga ctactttagg gaattcccta
    44161 cgtcgtattt tattatcttc tttgccaggt gctgccatca ctaatattca aatcgatggt
    44221 gtactgcatg aattctctac catcaaaggt gttagagaag acgtcactca aatcatcttg
    44281 aacattaaag gcttagcgct gaaaatgtat ggccaagaag aaaaaaccct tgaaatcgat
    44341 attaccggac ctgctacagt aactgctggc gatattatcg ttgatagtga tgtagaaatt
    44401 cttaacaaag atatgtacat ctgtagtgtc tctgaaggag ctacgttcca tgctcgctta
    44461 acagtgaaac ctggacgtgg ttatgttcaa gcagatgaaa ataaaaaaga agatatgcca
    44521 attggcgttc ttccagtcga ttcaatctac acacctgttc gtcgtgtgaa ctatcaagtt
    44581 gagaacacac gtgttggaca ccgtgaagat tttgataaat tgacgatgga gatttggaca
    44641 gatggttcaa ttgaaccgtt ggaagcaatg agtttgtctg cgaaaatcat gactgaacat
    44701 ttggatattt ttgtgaactt aactgatgaa gcaaaaaatg ctgaaatcat ggtcgaaaaa
    44761 gaagaaacac aaaaagaaaa aatgttggaa atgacgatcg aagagttaga cttgtctgtt
    44821 cgctcttaca attgtttgaa acgtgcagga atcaatactg tacaagaact tacaaataaa
    44881 tctgagcctg aaatgatcaa agtccgtaac ttaggacgta aatcattaga agaagttaaa
    44941 gcaaaattgc acgacttagg tttaggttta cgtaaagaag attaaaacct tttacgaagg
    45001 agggaaacca acgtgagtta tcgtaaacta ggacgcacat ctagccaacg taaagcgatg
    45061 ttgcgtgatt taacaactga tttattaatc aacgaacgta ttgtgactac tgaagctcgt
    45121 gcgaaagaag ttcgctcaac tgcagaaaaa atgatcactt taggtaaacg cggagatttg
    45181 catgcacgtc gtcaagctgc ggctttcgta cgtaatgaag ttgccagcgt gcgtgaagaa
    45241 aatgaggaca tcgtcattga atcagcttta caaaaactgt tcaacgatat tgcacctcgt
    45301 tacgcagaac gtcaaggcgg atacacacgc attttgaaga cagaaccaag acgcggtgat
    45361 ggtgcaccaa tggttgtctt agaacttgtc taaaatcaca ctaataacat cactttatag
    45421 atgattcttt tagagtgtta tgatgttgga agatattttt ccgagtctag ctcagcgctg
    45481 ctcctcaagc gaatatcgct tggggagtaa gcactcatca taaagtgttt ttttatacct
    45541 tcaattacct gtcatgacag atatgtttga aggcataaaa aaacatttta agcgagaaat
    45601 aattgaaaat tttgtctttt tttgatagtg ttagtaggaa ctttaggatg aagaaggagt
    45661 aatcatggag ccaattattg aattagaaaa gataaattat aaataccagc cagatgatct
    45721 tcgccccgca ttgaaagatg tctcctttac aattgataaa ggtgagtgga tcgctattat
    45781 tggacacaat ggttcaggaa aatcgacact tgccaaaacg atcaatggat tgcttctgcc
    45841 agaatcaggg atcgtgaaag tgggaaatca aatattggat gaagaaaata tttggacgat
    45901 ccgacagatg gttggaatga tttttcaaaa tccagacaat cagtttgttg gttcgacagt
    45961 agaagatgac gttgcttttg gtcttgaaaa tcaagggatt cccagagaag aaatgctagt
    46021 gcgtgtgaaa gatgcacttg agaaagttag aatggctgag tttgcttcac gcgagcctgc
    46081 tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc ggcgtggttg ccttacgacc
    46141 tgatatcatt attttagatg aagcaaccag tatgctagat ccagaaggtc gtgaagaagt
    46201 gatctctacg atcaagaaga tcaaagaaga aagtcaattg accgttatat ctatcactca
    46261 tgatattgat gaagcagcta atgccaatcg gatactagtg atgagacaag gcgaacttgt
    46321 ccgtgaaggt acaccaaaag aaattttttc tgctggtcct gaactgatcg acttaggtct
    46381 agatctacca tttcctgaaa aactgaagag cgcactgaaa gaacgtgggg ttgatgtgcc
    46441 cagtgaatat atgacagaag aaaggatggt ggattggtta tggacatccg ttttgaacaa
    46501 gtagacttca cttatcagcc gaatacacca tttgaacaaa gagccttatt tgacatcaat
    46561 atgacgatca aagaaaacag ttatactgca ttagtagggc ataccggaag cgggaaatca
    46621 accttacttc agcatttgaa tgcgcttgtt aagccaacaa gcggtacagt ccatattgga
    46681 gaacgagata ttcagccgga tacggataat aagaatttaa agcccatccg aaaaaaagtt
    46741 ggcatcgtct ttcagtttcc agaagcacag ctattcgaag aaacggtagc aaaagacatt
    46801 gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag cactagtcct agcaaaagaa
    46861 acattagaac aagttgggct ggatgaaagc tatttggaac gttcgccatt tgaactttca
    46921 ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg ccatgaggcc ggaagtactt
    46981 gtattggatg aaccaacggc aggacttgat ccccagggca gaaaagaaat gatggagatg
    47041 ttttggcgtc ttcataagga acagcagatc actattgtat tagttacgca tttaatggat
    47101 gatgtagcca attttgcaga ttatgtctat gtgttggaaa agggacgaat cgttaatagc
    47161 ggggagccgc aagaggtctt tcaaaacatt gaatggctaa aagaaaaaca actaggcgta
    47221 cctactgcca ccgagtttgc agaagaatta atggctaaag ggatgaactt tgctaccttg
    47281 cctttgacag ctgaagaatt agcagacgct atcgtccatt atgctggagg aaatgcccat
    47341 gatgaataaa ttgatatttg gtcgttatat gcctggagat tccttgatcc atcgattgga
    47401 tccccgagct aaattattag ccagttttta ttttatcgga atcatttttt tagcaaataa
    47461 ttgggaaacg taccttttgt tggcattttt cacattactt tctgtcttct tgtcaaaagt
    47521 agatttgaaa ttttttatcc gaggcattcg cccattgatc tggctaatcc tcttcactgt
    47581 agctttgcag gtcttgttta caagtggcgg agaaacttat tggagttggg gtgttttcca
    47641 tatcactgaa tttggtgtga tgaacggcct ctttattttc tgtcgattcg tgttgatcat
    47701 ttttatgtcg actctactca cgcttactac accgcctttg gaattatcgg atgcgattga
    47761 gtacttgctt cgtccattga aagccgtacg gttcccagtc cacgaaattt cattgatgct
    47821 gtcgattgct cttcgttttg tgcctacatt gatggacgaa acagaaaaga tcatgaacgc
    47881 tcagcgcgct cgcggtgttg attttggtga aggaaaccta gttcagaaaa tgaaagcagt
    47941 cattcctttg ctgattcctt tatttgtcag cagcttcaac cgtgcggaag atcttgcgac
    48001 tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga acgaaatatc gtgtattgca
    48061 ttggcataat agagacacga tggttatcat tgcttttgta ttattaactg tgctactgat
    48121 ctttttaaga ggataaacgt tcagtgggcc gctatctatt cttttcttgc tgatctgttt
    48181 gcaagaaagg atgcaagcag tgtatgacaa gaaatattag ggtctgtggc agactaaggt
    48241 cacaggcctc tttcttttat ctgaaaagaa agaacgaaaa aattttatag aaaaatcatt
    48301 attccagata accttttagg aacaaaggag agaaaaacat ggtacgttac aaagcaatca
    48361 ttgcgtacga cggaacaaat tttaatggat tccaaaaaca gccaaatggg cgtactgttc
    48421 aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg aaaagaaatc acggtctttg
    48481 gttcgggaag gacagatgcc ggagtacatg cgatcgggca agtaatccat tttgattatc
    48541 cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga tacccagtca ccagaggata
    48601 ttgctgtaag acaagtagaa attgtttcag aagacttcca cgcaagatat ttggtcaaag
    48661 aaaagacgta tcaatttcga gtggatatag ggaaaccacg cagtcctttt cgacgccatt
    48721 atgcaagtta ttttccttac ccacttgatt tggaaaaaat tcagcgggcg cttcctgatt
    48781 tattagggac tcacgatttc acctcttttt gtgcatcagg aagctcgata gaggataaaa
    48841 tacgtacgat ctacgaggct aagatggagg taaatgaaac aaaagacgaa ttgttgttta
    48901 catttagggg aaatggcttc ttgtataaga tgatacggat cttagtagga acgcttttga
    48961 aaattggcaa tggccgattg cctgaagata gtatcccaga tattattgcg aaaaaagatc
    49021 gtaatgcagc aggacctaca gcacaccctg aaggacttta tttgtatgaa gtgaaatatg
    49081 aataagaggt tatgatagaa gtggtcagct tcaagaaata agaaggtatt tacgaaaatt
    49141 acttctcaaa attttgcaaa atttcggctt attttcgaag aagctacttc tggaacaccg
    49201 tttaagaaag aggtcgtgag cctgtcattt aactctgagt cacaaagagc aatttcaaaa
    49261 aacagcttct catctttgat gaatatcgtg acttgtggac gaggcgttga taagtgaatc
    49321 atctttttgt tacctatttt ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc
    49381 cttggtgcgg acagtctgaa caggttccat cgtttcttta ttttctacgt cttcttctgt
    49441 gtgtgtttcg tggttgttct tctattgctg gatcaatcgt gccataaaag gggcgattgc
    49501 ggtcaaggta ccagacataa agaataaaca atgcaataca taggacaaaa aggattgcac
    49561 ctatcgtaaa tccttcaggc aagtaactga aagtgacagt atgtttacct gctggaactt
    49621 ttatcatcaa gaatgcgtct ttgaaattgc tgaccttgac tggtttccca tcaattttag
    49681 ctttccatcc tttgtcataa ggaatagttg tcaccaacat ggaatcttca gcggtattta
    49741 cagtcccaga agcttttctg ccagtggctt ccatttcgac acctttttct ttgatctgat
    49801 tcattgcggc ttgataggct acaacatcta gaccaacgac tttaggctcc atgaaactta
    49861 tttctttcgt cccataaaaa cttgcagtaa atgtcactgt tgtctcttga ggataatagc
    49921 caagatcata gtattgacca gaaatgttga tttgcgtttt tcgcgatgta ccattgacag
    49981 tgatcgtcgc actagaactt tctaattgcg caaaattggt tgggaacaaa cttaagtaag
    50041 cttgtgtatt tgcaggcaca tggacttccc aagtgagctc ttttggctgg ttatctgctg
    50101 tttctgagta agtgacgcca gtcgttgtct ttgtgatcgt cgtattcaac tgattcttca
    50161 aagtaggtgt atagaatgta aaatactgtt catttagacc agacaatgta ttgatcaagt
    50221 ttgtctgact ggtcaaatta tctgtcactg gctgctcaac atcattaacg gacaacggtg
    50281 cagtaaatgc tagtggcaat gcattgacat tctgatacag tgtatagtcg ccgctttgac
    50341 cagcttggcg gaagccatat ttactgaacg tgctttcgtt tttagaaatg ttatatttga
    50401 ttcctgtaaa cccatccatc agcaaagtat tattgttata acggatattc aaatttgtcc
    50461 ctcttgaacg gaaacctaat ttatccaggt aagaggaaga attacgattt cttatagaag
    50521 aaaacaaact gataccactg taaccatagt tgatcgaatc gtttgcagat actggatcca
    50581 gattttccaa gcggtaaaac gagtcggaat ctttttttgt ttcatttaca agagctttca
    50641 agtcaggata aggatcagta tataaactgc gggaagcata attccaatca tcaagaatgc
    50701 catggatcat actgttcgta ttgaccagtg cttcagcaga catgatcaat aataacaata
    50761 tactcagatg attgaatgaa agttttttat gctgataaaa tcccacagca aaagcataaa
    50821 tccctaagaa aaccactgtc aatacaaagg aagtcatcgt cacataggtg tagcttcctt
    50881 caggtttgat gccccatgcc accgcaaaga gagcagctac gaccaaaatg attcctaaca
    50941 acagcccacc atcgtcttgt tctatctttt cccatccttt tcctgcaagc aggagcacta
    51001 agaaagagaa cagataggca taacggaata aaaacatatt cggtgcatgc ataccatgcc
    51061 agaacaaatt caatggaaca agataaaaac ttgcgataag tatggcgaaa agactgccgt
    51121 acaaaaattt atttttcaac ggtactttcc gactggcaaa ataaaacaga cagaggacta
    51181 atggtagcag acctgcgtaa acgaacggaa tcgatccgta ttttgtcgtg tcataaacac
    51241 caatcatatt tttcatgatt agatccagat aagaagtcgc ttctgttttc aacgtagtga
    51301 tctgactcag tgcctcgcca tttgaccgaa gatccagcac tgctggcaat acaatgatca
    51361 ttgaagcgcc gcctgccagt aaagaagtga taccataagg aacaatcgtt tttttatatt
    51421 ctgaccagtt tgtcaataga cgggcgataa aatataagaa agaaaatacg ccaatcataa
    51481 aacccatata aaaactggat aaaaacaaga gtaaataact gataaataaa agtttcggcc
    51541 gtttaagatc cataacacga tgaatcccca aaatcactaa tggaagataa acaaatgcat
    51601 ccagccacat gatgatctcc gaatgtgcag tgataaatga gatcaatgcg taactgacgc
    51661 taagtgcaac atgcccccag cgaggaatct tgaacgtacg tgcaagaaac caaaaagcca
    51721 atccggcaca gccgacctta agcaaagtca gaacatacag tgcatctggc atcatctggt
    51781 ttggaaaaaa gaacaccagt ggcgtgaaga tcccgcctag atagtaagaa attaaagata
    51841 aataattcaa tcctagagaa gcattccatg tataaaaaat actttgcttt ccaagaagaa
    51901 catttcggaa gctggcatga aaattagaaa actgtgaaaa ggcatcactt gccaaaatac
    51961 ttctgctgct tccaggatag attccgatac ttagataaac aagtgctaaa atcaaaaacg
    52021 gaatcaggaa actagctgct gcgtagcgtc cgttttctct aaagaatgac tttaaatttt
    52081 ttttcatatt tattttccac caaatttcta tgaagttgta ttcagtttat catatccttg
    52141 tttttctctg cttaaaaaga gtctagtttt cacaaaaaaa taaaatttag aataaataac
    52201 ttcataattt gttcgtataa caaaaataaa cgcaaaaaaa gggtaggaat caaaagtccc
    52261 acccttaaaa atttttatta aataaaaaac cgttcactat ctgaacggtc caagtatcat
    52321 tcaattaaac gcgttcaact tttcctgatt tcaaagcacg agttgacacc caaacttttt
    52381 taggttttcc gtcgaccata acgcgaactt tttgtaagtt aggtttaaca gtacgttttg
    52441 ttgagttcat agcgtgtgaa cggttgtttc cactttttgt cttgcgacct gtaaagtaac
    52501 aaacttttgc catggtaatt tcctcctttg ccttgatttg gagcaataga ataattcagc
    52561 tcataccaga ctaacatatc atatagaatg ggaatttgca agtacatttt catagataca
    52621 attgacaatt gagaagaata attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg
    52681 tttttgagta ctttgggtaa ctgcctttta tttcaagttg cgctatgata aaatatgata
    52741 gaagaaaaat gggagttttt tacccgttaa ggaggctcat tcatggctgt aaaaattcaa
    52801 acgtcagctg gaaccattga aattaccaat gatgtaattg caacagttgt tggtggcgcc
    52861 gcgacagatg tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa agataatatt
    52921 actgagattt tgcgtaaaga aaattactca agaggagtag ttgtccgcca agaagataat
    52981 ggtattgctg tagatgttta tacgattatc agctatggta cgaaaatctc tgaagtttcc
    53041 cgcaacgtcc aagaaaaagt gaaatacaat ctagaggcat tattaggcgt aactgccaat
    53101 tcagttaatg tctttgtaca aggtgttcgt gtactgccag actaggcagg agactggctg
    53161 gtaactcagc agtcgatctg aaggaggatt atctaggtga gtgtaacaga aataagtgca
    53221 agccagttcc aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac
    53281 gtcaattcat taaatgtatt ccctgtgcca gatggcgata cgggaacaaa tatgaatcta
    53341 tctatgacta gtggagctaa ggcagtagta gattcaacct cagaaaaagt aggagaatta
    53401 gcagcaatgc tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg cgttattttg
    53461 tctcaattgt ttcgtggatt ttctaagcaa attccagatg ttgtctcatt aaatgcttct
    53521 gatcttgctg cagccttcac tcatggagtt gaaactgcgt ataaagcagt aatgaaacca
    53581 gtagaaggaa cgattttgac agtagctaga gaagcagcga aagctggaga aaagaaagca
    53641 aaaagtactg atgatgtcgt tgaagtaatg acagctgttg taaaaggagc aaagcgtgct
    53701 ttagctaaaa ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga
    53761 gggcaaggac tgctgtttgt ttatgagggc tttttaagtg cattgaatgg agaattccaa
    53821 gcggatgaaa catatgaacc ttctccagca gaaatggatg atatggtcaa tgcagaacat
    53881 catcgcagtg tccaaggtca aatggcgaca gaagatatcc attttggtta ctgtacagaa
    53941 atcatggtaa aaatcggaga aggacctaca gtcgatagta cattcgatta cgaagaattc
    54001 cgtaactatt tagatggtat aggagattct ttacttgtgg tcaatgatga cgagatcatc
    54061 aaagtccacg tgcatacaga aaacccagga gaagtcttga attatggaca aaaattcggt
    54121 tcattagtca aaatcaaagt cgacaacatg cgcttgcagc atgagacgat tttggaacac
    54181 gatgaagaag tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta
    54241 gcgatcgctg ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt
    54301 atcagtggtg gacaaacaat gaatccaagt acggaagaca ttgttaaagc gattcaagaa
    54361 gtaaacgcag ataaagtgat tgtcttgcca aacaataaga acatcttcat ggcagctgat
    54421 caagcagcag aagtagcaga tatcccagta gcagttgttc cttcaagaac gatttcccaa
    54481 ggattgacag ctatgctttc attcaatgat caagcttctt tagaagaaaa caaagaaacg
    54541 atgacagaaa tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc
    54601 tcaattgacg gtgtagagat caaagaagga gactttttgg gcatgatcga tggaaaaatc
    54661 ctgatctcta acccagatat cacagcaaca gcgttaagta cactagaaca aatgatcaat
    54721 gaggacacag agatcgtaac gatcttggca ggtgatgaag gaacagttga acaagcaaat
    54781 gaatttgctg agaaactaac agaaaaacat cctgatttgg aaatcgagat ccaccaagga
    54841 gatcagcctg tctatcctta tctattttct gctgaataat ttctgtgtca gaatatcttt
    54901 ctaacacagt attcattcat aaaaggcctt cggttagtga tcaggtatga tcgctgacca
    54961 agtccttttt ttgttaaagg agggaggaaa aatttatgcg ttctttagat gatccaatca
    55021 ctatgctggc aggagtcgga gaaaaaagag cagctagtct tgccagcttg ggcatccaaa
    55081 cgattgaaga cttactgact tattatcctt ttcgctacga agatattcag gaaagaaatc
    55141 taaacgagat ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg
    55201 tgatgaatcg atttggttat aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg
    55261 atgtgttcaa cgtttctttt tttaatcaac cttatctaaa agaaaaagtc attttatcgg
    55321 aggaaatcgc cgtatacggt aaatgggatg caaagcgtaa agccttgaac gggatgaaga
    55381 ttttaggttc tcaaacaatg gatgactttt cgccgatata tcacgtcaac aaatcaattc
    55441 gtcaaacgac attagttgat ctaatcaaaa aagggtttca agaatacggc acattgattg
    55501 aagaaaatct gccaaacaac cttgtggaga aatatcgact gcttgaccga gcttctgctg
    55561 taagaagtat gcattttcca aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg
    55621 tttttgaaga gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag
    55681 cagaaacgaa tggtttggag atcctttatg atgtccaaaa attgaaacaa tttactcaaa
    55741 agctaccttt tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc
    55801 ttcgcagtcc gcagcatatg cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag
    55861 tggttgccgc gattgcgtta tacgctaccg ttacagcagg tttccaaggt gcgttgatgg
    55921 tgcctacaga aatcttggca caacaacata tggaaagctt gcaacagctg tttgatccca
    55981 atgaagtaac catcgcattg attacggggt caacgaaagc aaaagaacga cgtgagctcc
    56041 ttgaaaaact agagcaaggg gaaattaacg tcgtgatcgg cacacatgca ttgatccaag
    56101 atggagtcga atttcagaat ttaggtctag ttattacgga tgagcagcac cgttttggcg
    56161 tgaaccagcg gaaagtactt cgtgaaaaag gctggcgacc agatgtctta tttatgacag
    56221 cgactccgat tcctagaaca ttagctatta cagcatatgg tgagatggat gtatctatca
    56281 ttgatgaact tccagctgga cgtatcccga tcgaaacacg ctgggtaaga acacctcaat
    56341 tggacagtgt actggattgg acgaaaaaag aactggcaag aggacatcaa atgtatgtca
    56401 tctgtccgct gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg
    56461 aaaaacttcg tgaactattt gcaccaaaat atgaagttgg cttgttacat ggtaaaatga
    56521 agaatcagga aaaagaagta atcatggaag ccttcaaaga caaccagatg cagatattag
    56581 tttcgactac tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca
    56641 tggatgcgga tcgatttgga ttggcacagc tgcaccaact tcgtggacgt gttggacgcg
    56701 gaagtgatgc ttcctactgt atcttagttg ctaatcctaa aaatgaacta ggtgtggaac
    56761 ggatgaaaat catgacagag accaccaatg gttttgtact aagcgaaaaa gatctggaat
    56821 tacgtggtcc tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg
    56881 ctgacttggt gacggatgga aatgttttgg aggtagccag agaagaggct gcagcccttt
    56941 ggcaaataaa ggattggcag atgcttcctg agtatcagcc tttagcgcaa agcctccaaa
    57001 tagaaaatca ggataatcgt tatttcgatt agcagactgt gctatacttt atactgatga
    57061 cggaaaaagg agtggaaaca atgaaaattg ccgtagatgc aatgggcgga gatcacgcac
    57121 ctcaagcaat tgtagaaggc gtggctttag cgcagcaaga ttttccagaa gtagaattct
    57181 tactttacgg aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa aacatcacga
    57241 ttatccatac agacgaaaaa atcaatagtg atgatgagcc agtaaaagca attcgacgaa
    57301 aaaagactgc atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg
    57361 tattttcagc agggaataca ggcgcgctac tagcagcagg gttgttcatc gtaggacgta
    57421 tcaaaaatat cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg
    57481 ccggtttcga tatgctggat cttggcgcta acgcagaaaa caaaccagaa catttgttgc
    57541 aatacggtat tttaggttct ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg
    57601 tcgcactctt aaacaacgga acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct
    57661 atgaactcct tcagaatgaa acaagcttga attttattgg aaacgtagaa gctcgtgagc
    57721 tactcaatgg tgtggctgat gtagtggtca cagatggatt tacaggcaac gctgttttga
    57781 aatctattga aggaacagca atgaatatga tgaatctatt aaaatctgct attctaaatg
    57841 aagggatcaa aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag
    57901 cagaaatgga ttattccaaa catggcggtg ccgttttgtt tggcttgaaa gcaccagtca
    57961 ttaaaactca tggagcaaca ggccccgatg ctgttcgcta tacgatccgc caaatccaca
    58021 caatgcttga gacagatgta gttggacaat tagtcgaaca atttgaaaaa agcgaagaat
    58081 agtatttgga tatctgaagc gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa
    58141 tgccagtaaa attagccatg gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa
    58201 aagttgactc gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag
    58261 atagataccg ataaagtgac ggatgaattg aacatcaaag atgatttaaa agctgattca
    58321 attagtatta tggaattcgt tttagagctt gaagatgaat tcggtacgga aatttcagat
    58381 gaagacgctg aacaaattga aacagtcggt ggcgcagtag attatatcag cagccatctt
    58441 aaataatttc aaataaaagc aaagaaggat aaaaagctgc atgcagcttt ttatccttct
    58501 ttttttaatc tttttgacac gtaaaagtca tttttctgat aattctggcg ataaacagaa
    58561 attatgttgc aaagtaaagc ggttttctat ataattatta ataattcatt acacaaagat
    58621 aagaataacc tttgttataa aacgatttat tctgtgtaat gtcctttttt attaagaaaa
    58681 acttccattt aggaagcaga gaggggtatt cgattgtccg ataaccaatt attagaagtt
    58741 cagaacctac atacaggatt ccgactcaaa gatgactatt acgatgcagt agatgacgtt
    58801 tcatttactt tagacaaaaa cgagatttta gcaatcgttg gggaatctgg gtgcgggaaa
    58861 agtaccttgg ctactacgat tatgggatta cttgatccta ataacacaaa aattacaggt
    58921 gagattttat ataacgatct gaatttaatc gacctgaacg aaacgctcta taataaaatc
    58981 cgcggaaatg atatcggaat gattttccaa gatccgttgt ctgcattaaa tcctctcatg
    59041 agaatcgaag accagatcaa agaaggactt tcctatcaca caaagatgaa tgcagaacag
    59101 cgtcaagctc gtgcactaga attgctagaa caagtaggga ttcctaatcc tgcgagagtt
    59161 ggacgccaat atccccatga actatcaggc ggtatgagac aacgtgtcat catcgcaatc
    59221 gcaatcgcct gcaaacctcc aatcgtcatt gcagatgaac cgacgacagc cctagatgta
    59281 accatccaag cacagattct tgatttactg aaagaccttc aggaagaaac aaaaacaggg
    59341 atcatcttga tcactcatga cttaggagtc gtagcagaaa tggctgatcg agtggcagtg
    59401 atgtatggcg gacagttcgt tgaagtagca ggtgtaaaag aactgtttga tcatcctaag
    59461 catccttata cacgctcttt actgaactct ataccacaag aaggaaatca tgatgcagaa
    59521 ctccatgtga tcgaaggtgt cgttccttct ctgaaaaaca tgcctcgtaa aggttgtcgg
    59581 tttgcaccac gtattccatg gataccagca agcgatcatg aagaagagcc tatccttcat
    59641 gaagtcgcac cgaaccactt ggtacgttgc tcctgctata agcatttcca ctttagagac
    59701 gaaaaaggag aggtgtagat gacagaatta atcacaatca aagacctgaa agttcattac
    59761 cctattcgca gtggattctt caatcgagta acggatcatg tatatgctgt agatggtgtg
    59821 gattttatta tcgaaaaagg aaagacatat ggtttagtgg gagagtctgg ctctggaaaa
    59881 tctactacgg gaaaagccgt tgtgggatta gaaaaggtaa cagccggaca aatcatgtat
    59941 gaaggaaaag atgtaacgaa gcgaagcaac cgcaaaaaaa tgggttataa caaggatgtg
    60001 cagatgatct tccaagattc aatgtccagt ttgaatccta aaaagcgagt gttggatatc
    60061 attgctgaac ccatccgaaa ttttgaacgg ttaagcgatc aagaagaaaa gaaaaaggta
    60121 aaaggactgc tagatatcgt tgggatgccg gaagatgcgc tatataaata tcctcatgaa
    60181 ttttcaggcg gacaacgtca acgtctagga gtagcccgtg ctgttgctac caatccaaaa
    60241 ctgatcgtag ccgatgaacc tgtatctgct ctggatctat ctgttcaagc gcaagtattg
    60301 aatttcatga aacgaatcca gcaagaatac ggcctcagct atttattcat ttcacatgat
    60361 ttaggtgtag taaaacacat gtgcgacaat atcgcaatta tgtacaaagg tcgatttgta
    60421 gaaatcggta caagagaaga catttataat gacccgcgac atatctatac gaaacgtctg
    60481 ctttcagcta ttccgcaaat cgatgtagaa aatagggaaa gccacaaaga aaatcgacga
    60541 agagtagaac aagagtatat ccaaaaccaa aaagaatact atgatgcgac tggtcgagtc
    60601 tacgatttgc gtacgatcac aactacacac aaagtggcgt tgaaagatgg aggtgcaagc
    60661 taatgtggaa aacgattctg cgacgtgttt tattgatgat cccgcaagtt attattttaa
    60721 gtgttctgat cttcttatta gcccaagcga tgccggggga tccatttacc ggtttgatca
    60781 atccaaatca ggatccggca gtgatcgaac agatgcgaca agcagctggc ttgaatgacc
    60841 catggtatgt ccagtacttc cgttggatcg gaaatgcttt gcaaggcgat tttggtcaaa
    60901 gtttcttata caaacgctct gttgctgact tgatcggcga acggatcatc aacactttat
    60961 atctatcgat cttgacagtt gtccttactt atttgattgc tgtgcctctg gggatgcttg
    61021 ctggccggta tcaaaattca atcttggata agattgtcgt aatctacaac ttctttagtt
    61081 ttgctgttcc tttatttatc ttcggcttga tcatgctatt tgtttttggt tatcgtttag
    61141 gctggttccc aacaagtgga tcacaaacac taggtttgca aggtggattt atcactcaat
    61201 ggatagaccg tttgaaatat attctgttac catcaattac ccaagcattt ttggcgactg
    61261 ccgtcacaat ccaatatctg cgaagtgaag taatcgattc gaaatcgctc gattttgtca
    61321 gaacagcccg ttccaaaggg gtaccgacga ataaagtatt cagtcgacat attttccgaa
    61381 atgcagcgtt gccgatggct tctcaaatgg ggtatgaaat cacttcgttg attgctggtt
    61441 ctgtagttat cgaaaagatt tttggttatc cgggagtcgg aaaactattt attgattcca
    61501 tcggccaacg ggactatacc gtgatcacat ccttagtatt gattttagga atcgcgacgc
    61561 tagtggggac attactttca gatatcatca tgagtatcgt tgacccgcgt attcggattc
    61621 aataaaacca atcagaaagg agaatggatc gatgagtgaa aaaaacaata cagcagttca
    61681 agaaagtatt cctcctatgg ggatacgaat gattgctcga gaatttaaaa aagataaaat
    61741 tgctattttt tcattaggtt tgctagtggt tttattatta gtgatcttta tcggtgcgat
    61801 gctgaccgat caagacaaag tgatgtatgt tagtatcttc gacaaatacg ctgcaccagg
    61861 aacagtttcg acacaaggac aaaagttcat tctgggagca gatgaaggcg gacgtgatgt
    61921 attaggacaa ttgatcatag gtgctagaaa ctctgttacg atcggatttg cgatcacgat
    61981 ccttacttcg atcatcgggg ttggattagg gatcatctct ggctactacg gagggatcat
    62041 tgacaacatc ttgatgcgga tcgttgattt tatcatgatt ctaccgatca tgctgatcat
    62101 tatcgtcttc gtatcgatta tttccagata caatgtctgg tcctttatat tcatcatgag
    62161 tgcattttat tgggtagcta aagcccgatt attccgaagc aagacattat ctgaagcaag
    62221 aagagattat gtcagtgcct caaaaacaat gggaaccaat gattttaaaa tcatgttccg
    62281 cgaaatcatg ccgaatctaa gttcattaat cattactaat ttgacgatga actttgcggc
    62341 aaacatcggt atcgagacaa ctttgacttt cttaggtttc ggcttgccgg caaatgtacc
    62401 tagtttaggt acattgatcg gttatgcaag taacggagaa gtcttagtga atcggcaatg
    62461 gatctggtta cccgcatcaa tccttatttt agttttgatg ttgagtatca attatgttgg
    62521 acaagcattc aagcgttctg cagatgcgcg ccaacggtta ggctagtata gaagcgaagg
    62581 gggaaatagg aatgaagaaa acgatttttg gatttgcagc gctgatgtct gctgtcgttc
    62641 ttgcagcatg cgggggcgga ggtacagata cctctggaaa caactctgga ggcaacggca
    62701 ataacaaaga ggaattaact tttccaattg caacaaaaaa taatgatgat gcaatcgaag
    62761 acggcgaatt agaagctgcg gtcgcaacgg acagtcagtt taaaggattg ttccagtggg
    62821 agttttacca agattcatat gatgcagctt ttatggctcc ttctcatgaa ccgctatttg
    62881 caaacgatga cgatttcaga atcacagacg acggagctgc aacgcttgat ctagatgaag
    62941 atacgaaaaa agcgacaatc aaagtaaaag acaacgtcaa atggtcagat ggtgaagatg
    63001 taacggcgga cgatgtgatt ttcccttacg aaatcattgg gaataaagat tacactggta
    63061 tccgttatga cgacactttc cgtaacatcg taggtatgga agaatatcat gatggaagtg
    63121 ccgacacaat ctccggaatc aaaaaaatag acgacaaaac agttgaaatc gaatataaag
    63181 aagtaaaccc aagcatgctt caatcgggtg gtggtatctg gtcatatgca gcaccaaaac
    63241 atacgttaga aggtgtagca atcaaagata tggaatcaag cgatcaagta cgtaaaaatc
    63301 cagtgacttt tggcccttat tacatgagta atatcgttgc aggagaatca gtagaatact
    63361 tgccaaacga gcactactgg aatggtacgc ctaaattgaa aaagatcacg atgaaatctg
    63421 taccgatggc aagtgcaact gaagcattga actctaaatt atatgatatg attttccaaa
    63481 tgccaacaga tacttacgat acctataaag aaattgctgg ctatacaaac ttaggccgtg
    63541 aacaaacttc ttatacttat ctaggattca agttaggtaa atggaatgca gatacaaaga
    63601 gtgtggaata caatccagat tcaaaaatgg ctgataaatc attacgtcaa gcaatgggct
    63661 atgcattaga taataatgca gtaggtgaac gtttctatgc aggtttgaga agcaatgcga
    63721 cttcattgat tcctccagta ttccagtcat accatgatga tgagatgaaa ggattcacac
    63781 aagatctaga caaagcgaaa aaactattag atgatgctgg ctacaaagat gtggatgacg
    63841 atggtttaag agaagacaaa gatggaaata aactaacaat caactttgct tcgatgtccg
    63901 gtggggaaac agcgcaacca ttggcagatt actatatcca acaatggaaa gaaattggtt
    63961 tggatgtcca atacacaaca ggtcgtctga tcgaattcaa ttctttctat gataaattag
    64021 aaaacgatga tcctgaaatt gatgtttacc aagctgcttg gaatacagga actgatccaa
    64081 atccaagtgg actatgggga ccaaatgctg cgttcaacta cactcgttat gaatcagaag
    64141 aaaatacaaa attgatcaac gatatcagtt caagcaaagc atttgatgaa gagtaccaaa
    64201 aagaagcata tcaaaaatgg caacaatatg catttgaaga agcatttgcg attccaacgt
    64261 tgttccgtaa tgaagtattg ccagtcaacg accgtgtcaa agactgggat tggtcttatg
    64321 atgtaccaaa cccatgggca aatgtttctg ttacagcaga ttcacgttca taatcactaa
    64381 aaatgaccga aaaagggcga tgtccttttt cggttttttg atatttttct gtttatttct
    64441 ctaatattca ggtcaaggcg ctgatttcat gacttctcca ataaacggtg ttcaaagtcc
    64501 agtttctcgg tattaagtca aatttatgca aaaatatgga aagccatttt cacaaaattt
    64561 tttcttcata ctcgaaacta aacgaatttt tcacaacttc tcgtcaatca ctaggattat
    64621 taaagcttta cttacctttt gaccgaacca tttcaaattt gacaaaaaac cagtataata
    64681 aggaagtatc gaaaaaatat ccataaaagg aatgtggaaa atggacaacc agctgacaaa
    64741 agaactaaaa gaaaactata acatcgtttt tcacgatcta aatttgcttg aacaagcttt
    64801 tacccattca tcctatgtga atgagcatcg caatctgcaa ttatcggata atgagcgact
    64861 tgaattttta ggagatgccg ttttagaact gatggtttct cagtatttat tccgtttata
    64921 tcctcatttg ccagaaggaa aattgacaaa aacacgagct gcgatcgttc gcgaagacag
    64981 cttgtcgaaa tttgccaaag aatgtcactt tgatcaatat gttttgttgg gcaagggaga
    65041 agagaattca ggaggacgta cgcgaccagc attgctttgc gacctattcg aagctttttt
    65101 aggcgcgtta tatcttgacc agggattcga cacgacccat gcattcatcg aaaaagtgat
    65161 ttttcctaaa gtcaaagcag gtgctttttc acatgagatg gatcataaaa caaaactaca
    65221 agaagtcttg caaaaatcag gtgatgtatc aatcgaatac cgattgatca atgaagaagg
    65281 tccagctcat gaacgagtat tttggatcga agtttatgta gatgatcaat tgattggcac
    65341 tggacaaggg aaatcgaaaa aattagcaga acaagctgcg gcggaaaatg cattggcagc
    65401 actgtaaaag taagtgaggg aaagctttgt atttgaaacg aattgaaata gccggtttta
    65461 aatcatttgc agacaagaca gtcattgatt ttgaaaatag cgtcacagca gtggttggac
    65521 caaatggcag cggaaaaagc aatattacag aggctatccg ctgggtatta ggagaacaat
    65581 ctgctaaaag tctgcgaggc ggaaaaatgc cggatatcat ctttgccggt tccgataccc
    65641 gtaagcagct gaatattgca gaagtcacag tgattttaga caatagtgat cattatctgc
    65701 cattggagta caatgagatc agtgtaaccc gccgttatcg acggactggg gaaagcgagt
    65761 ttttcatcaa taaacaaagc tgcagattaa aagatatcca agaattgttc ttagattctg
    65821 gattaggaaa agaatcgttc tcaatcattt ctcaaggaaa agtagaagcg attttcagta
    65881 gtaaaccgga agaccgtcgt gggatctttg aagaagcagc tggtgtcttg aaatacaagc
    65941 agcggaaaaa gaaagcagaa cagaagcttt ttgaaacaga agacaacctc agccgcgtcc
    66001 aagacatcat ccacgaattg gaagaacagt taactccttt ggccgcccag agtgaggcag
    66061 caaaggagtt tttacggcta aaagaaacac tgactcaaac cgatgtctct ttgatggttg
    66121 ctgaaatcaa gacagcaaaa aaagattggg acaacaaaca agcacagctt gcgaaattca
    66181 atctagaatt aggcaaacta tctgagtcga tccaagaaca agaatccatt ttggcgaagc
    66241 agcgtaaaga aaatgcgcaa gccgatcgtc tgatcgaaaa aaatcagcaa gtattattag
    66301 atctttcgga aaaactgaag cagacagaag gacaaaaaga tgttttgcag gagcggacaa
    66361 agcatacaca aaaaagctcg caagaatatc aaacttcatt agcagaagca caaaaaaagg
    66421 ttaaacattt tgaaaagctt caagaaagct tgatgaaagc agcagcagaa aaagaaacag
    66481 agatccaaaa agctgaagca aatctcataa aaacacagca agaattagaa aaataccaaa
    66541 aatcaacgaa agaattattg gcagaattaa gagatcaata tgttgatttg atgcaagaac
    66601 aagcagctgt aggaaacgaa ctgaagtatc tagaacgtca atatatacaa gaaacagcaa
    66661 aaagcaagca gacgttagcc aagcaatcgg aagtagaagc atcagtagac cgattgatct
    66721 tacaaaaaga agaattgaca caaaagcagg cgcagctaaa atcatcttta actgaaacaa
    66781 aagagaagtt agaaatgatc caacaaaacg ggaaaaagtt ccaagaagca ttagcaaaag
    66841 aacagcctaa aatgtatcaa ttaatgaatc aagtccagca gctacgtgct cgtaagaaaa
    66901 gccttcaaga aatccaagaa aactattttg gtttttacca aggggttcga ttggtcttgc
    66961 agcataaaca gcagctttcg ggtattgtcg gagcagtggc tgaactgatc gatgttcctg
    67021 cagatttcac gttagctatc gaaacagctt taggtggggc agcccagcat gtcatcgttg
    67081 aaaatgaaaa agatgctcgg caagccatca cgtatttgaa acagcaaaga ggaggaagag
    67141 caacattcct tccactgacg acaatcaaac caagacaatt accggctcat atattgacac
    67201 aagcagcagc tgtagaaggg tttataggga ttgccagtga acaagtcagt tatcccgatc
    67261 aaatccaaac tgtggttcaa aacctgctag gaacgatcct tttagccaaa gacttaacta
    67321 gtgccaatgc aatcgcgcaa acgatccgct atcaatatcg agtggtttcc ttagaaggtg
    67381 atgtgatgaa tgccggagga tccatgacag gtggagcgaa taaacgagga aaccaaggca
    67441 gtttgtttgt acaaaatcaa gaattgaaac agttgactag tgaatttgaa gaagcagata
    67501 aacaactcca agcacaagaa aaaaaagttc aggagctgca acaagaaaca gctcgtttag
    67561 ccgaagaaca agaagttttg cgtacacgag gcgaacaatt acgttttgaa gaacaagaag
    67621 cgacaaatca gctacaaaac atcataaatg aacttgaacg ttttgaaaaa gaaaagcaaa
    67681 tctcaacttt tgagacacgt gaactgcagc agtttatcga agattacgaa aagcaaacga
    67741 acgaattgaa agacaaacaa acagatcttg aatcgcaacg acaaaaaatc gacgaagaaa
    67801 tcaaatcgtt aagtcaagaa agcgaccaga tggaagccag aagagcacaa gtacaaagtc
    67861 aaaaagcaca agaacaagca gatcttgctg tcttgaaaga gcagtttaat catctgcaga
    67921 tccagctccg cggtgcccga gtccaaaaag cagaggcaac cgaacgtcaa gaagcgatag
    67981 aaaagcagtt ggcgacattg acagcagact tttctgatca tgaagtcaca gaagaaagtt
    68041 tggaaaaaca aattaacgaa ttatctgctc agcgagaaac attaaaagcc gagttggcaa
    68101 aagccaaaga gcagcgagat agaaggcaaa aggagatcga taagcttgaa gcagtcttgg
    68161 cagagcgtaa tcgtgagcaa aaagcacgac tgtcggaaca atccaaatta gaagtccaaa
    68221 aagaccgagc tgaaatgatt ttagataatc atttgaccta tctgcagtca gaatatcagc
    68281 tgacatttga gaaagcttcc caagactatc aagaaacaac cgatatcgaa gacagtagaa
    68341 caaaggtttc atccttaaaa gaacagattg aaaaactggg accagtaaac ttaaattcaa
    68401 tcgaacaata cgaacaagtc agtgaaagac atacattctt agcaacacag cgagatgatc
    68461 tgttagcagc gaaaaaccaa ttgtttgaaa cgatggatga gatggatgat gaagtgcgga
    68521 caagattcaa agaagtattt gaagctatcc gtcaagaatt caaaattgtc tttccaaata
    68581 tgtttggcgg cggtaaagct gaattagtac tgacggaacc aactgatttg ctgaaaacag
    68641 gtattgagat tgaagtacaa cctcctggca aaaaactgca aagtctaagt ttgctttctg
    68701 gaggagaacg agcacttaca gccatcgcgt tgctcttttc cattatccgt gtctgtcctg
    68761 ttccgttttg tatcttagat gaggtagaag cagcgttaga tgaagccaac gtgaaacgtt
    68821 ttggtcgtta cttgagcgat ttccaagatg acacacaatt tatcgtagtc acacaccgca
    68881 aaggaacgat ggtcgcagca gatgtactgt acggagtgac gatgcaggaa tccggtgtat
    68941 caaaaatcgt ttctgtacgg atggaagata tcaatgaaga ggcgaaaatc aacgaaacga
    69001 aagtgtagga acgaaaatga tcaaattagt agcaatcgat ttggacggta cattattaaa
    69061 tgaacaaaag ctgatttcag atgagaataa acaagcatta gcacaagcaa aacagcaagg
    69121 ggtcaagatc gttctatgta caggacgccc cttagctgcg atggcgcatt atctgcaaga
    69181 attaggactg gtagatgaag gtgactacag catcactttc aacggcggtt tggtacaaaa
    69241 aaatgatact ggtgaaatca tggaaaagaa agtgatggaa gtatcagata tccaacgctt
    69301 atacaaactg gctcagcaat tggatctgcc actagatgtt ttgtctgaca atgtggtttt
    69361 acagctgcct agtgccccaa ggaagcaatc cttgtacaat gtgttgaata agctgctgca
    69421 attccaacca gcggatataa gcgatattac agatgaattt gtgcttaata aggcagtaat
    69481 cgcttatgac caagaagaat tagatcctaa aatcaaagaa ataccagcag aattccatga
    69541 aatgtatgag atcatcaaaa caagaagtat gcttttggaa tttatgccaa aaggtgttac
    69601 aaaagcttat ggtatttctt tattggcaaa agatcttggt ttgaaacaag aagaaatcat
    69661 ggcgatcggt gatgaggaaa atgatttacc gatgatccaa tatgctggac taggcgtagc
    69721 gatggagaat gctgtgcctt tcgtcaaaga agcagcagat ttcgtgacag ccagcaatga
    69781 agaaaatggc gtagccaaag tgatccaaaa atttgtatta gagtaggggg agtcaaatgg
    69841 gattttttga taaaatcaag cgtgctttta ctggcgaaga gaaaacagaa gaagttgaag
    69901 tacaggagaa gtacgacaag ggtttagaaa agacgcgtaa aacatttggt gaacggatga
    69961 atgagttgtt tgccaatttt cgaacagtcg atgaagactt ttttgaagaa ttagaagaaa
    70021 cacttatcgg tgcagatgta ggttttgaaa cagcgatacg aattaccgaa gcattacgtc
    70081 aagaagtgaa actcagaaat gcgaaaaaat cggcagaagt ccaaaatgcg atcatcgaga
    70141 aacttgtgga tctctatgaa gcggaaggaa tcaatgaagt aaatgaacta aatatccaag
    70201 aagacggact aacagttatt ttgtttgtcg gtgttaatgg tgtagggaaa acaacaagta
    70261 ttggtaaact tgcccatcag ttcaaaaacg aaggtaaaaa agtattgatg gcagcagcag
    70321 ataccttccg cgctggagca atcgaccagt tagttgtctg gggcgaacgc gctggtgttg
    70381 aagttgttcg tggcaatgca ggaggcgatc cagcagctgt cgtattcgat gctttggaaa
    70441 gagcaaagac agagcaggca gatgttttac tagttgatac tgcaggccga ctgcaaaata
    70501 aagtgaactt gatgaacgaa ttagaaaaaa taaaacgagt aatccagcgg gaattaccag
    70561 aagcacccca agaaacactt cttgtattag atgcaaccac tggtcaaaac gcgatggttc
    70621 aagcaaaaca attcaaagaa acaacagatg tgaccggtct tgttttgaca aaattagatg
    70681 gtacagcaaa gggcggtatc gtacttgcta tccgtaatga attgcattta ccagtgaaat
    70741 tggtcggtct tggtgaagga atcgatgact tggagccgtt tgatccaaat gattttgtcg
    70801 ttggattgtt caaaggatta ctaaaagaag aataacccat gtctaaaaag caggagctgt
    70861 gacaaaagtt gcagctcctt gtttgaatga ttgtttttaa catagctgtg tttttgatgc
    70921 cttcattttt atacttattt gtaagcggtt ctcaaacaat ttcgttcttt ttcttagact
    70981 ttttgattat aattagattc atatgaattg gaggtcactt aatgatgaat tttaagcaat
    71041 tattattgca tgtcaatgcg cgccctttta tcgaccaagc tcgatttggt atagaacggg
    71101 aaggacagcg ggttgatctt gcaggaaatc tagcaaaaac cgatcatcca gcaatctttg
    71161 gcgatcgatc ctatcacccc tatatccaaa cagattttag cgaaacacaa acagagatga
    71221 tcacccctgt taccgattct attcccgaat tatttcagta tctcgctgct gtttatgatg
    71281 tgactgctcg ttctataccg aaagaagaga tgatctggcc attaagtatg ccacctgcct
    71341 taccggaaaa agacgaagag atcattattg caaaattaaa aaatttcgaa gatgtcttgt
    71401 atcgacgtta tttagcaaaa gaatacggga agcgaaaaca gatggtaagc ggtattcatt
    71461 tcaattttga atttggcgat gagttgctaa gaacattgtt cagccatcag gaagaatttc
    71521 aagatttttc cgaattcaaa acagaacttt atttgaaaac agcgagaaac tttatgcgct
    71581 atcgctggat gatcacttat ttattcggtg cttcaccaat gagtgagaag aactactttt
    71641 tagatgaatc acatccgcaa gaacctgttc gcagtattcg caacagtgcg ctgggctata
    71701 cgaatcatcc aaatgtgaaa gtttcctatg cttctatgaa acagtattta gcagatatcg
    71761 agcgaatgat cgaagaaggg aaactttcgg aggagaaaga attttataca ccgcttcgtt
    71821 tccgaggcgg aaagaaagtc gcagatttag caacaacagg tgttcgttat attgaattgc
    71881 ggaatatcga tttgaatcct tatgcaagat tgggtatcaa tccagagcaa gttcggttct
    71941 tacaattatt cctgatgtac atgttatgga cagaagaaaa agaagactgt gaccaatggg
    72001 tagcagaagg tacgactcga aataacaaag tagcactaga acaaccatca gatcaaacag
    72061 aatttcatca agaaggtaga gaaattcttg aaggaatgaa gcaaatgctg gttgaattgg
    72121 attggttgga ttctctttat ttagtagagg aagcattgac tcaaatggat catcctgaac
    72181 aaacgttagc agcgaaactc tatcaagaag cacagctatc gagtcagcaa gaagttgctg
    72241 tggcattggg acatcaatat tataaagaaa gccatgaacg tccctatcag ttggccggtt
    72301 ttcgcgaaat ggaactttct actcagatat ttatgttcga tgccatccaa aaaggtgtcc
    72361 aagtcaaagt attagatgag tcggatcaat ttttgcgtct gcaattccaa gaccatgtag
    72421 aatatgtgaa aaatgcaaac atgacaagta aggacagcta tatcgtccca cttatcatgg
    72481 aaaacaaaac agtaacgaaa aaagtcttaa aagaagctgg gttccgggta ccaggaggcg
    72541 cagaattttc atccatggaa gaagcggtaa aagcttatcc gagatttgcc gagcaggcat
    72601 ttgtgatcaa acctaaatca accaattacg ggttaggtat cacgattttc aaggaaggag
    72661 ctagtctgga agattatcaa gcaggtttag ccattgcttt tcgtgaagac agttcggttt
    72721 tagtagaaga atttatgccg ggaacggaat atcgtttctt tgtgatcgac ggagaagtcc
    72781 aagccatcat gttgcgagtc cctgctaatg ttattggtga cagtatccga acagtgaaag
    72841 aacttgttga agaaaaaaat agtgaccctt tgcgtggaac gaatcatcgt gcacctttag
    72901 aattgatcca attaggtgaa ttagaacaat tgatgttgaa agaacaaggg ttgacgatcg
    72961 aatcagttcc ccaagccaat caaattgttt atctaaggga gaactcaaat atcagtacag
    73021 gtggtgattc gatcgatatg actgatgaat tttctgaagc ttacaaaaaa atcgccgttt
    73081 ctgcggtaga agcactagga gctaagatta gtggtatcga tttgattata ccagataaag
    73141 aaatcgatcc tacaactgat aaaaaagcat atgggatcat cgaagcaaat ttcaatccag
    73201 ccatgcatat gcatgtttat ccttttgcag gaaaaggcag aagattgacg atgaatgtat
    73261 tgaaattgtt atatcctgaa gtattttaaa agaaaaagct tttgacagat tgtcctattt
    73321 gatgtcaatc tgatcaaaag ctttttttgc atgtaaaaat ttatgtgttt tttcgtttta
    73381 aaataaacga tcccagaagc tttttttctt ttttggtttt tctttttcag ctggcgaaga
    73441 gttttcttct gggtattttt tttcgatatc aatcacaggt tcatttactg cttcttttgc
    73501 tgttaagagc aatccgaatt cctcaggatt tgcagtgacg taatcgttga ctacagtgaa
    73561 acggctattg tttttcgttg ctagctggat atattggttt tggacagaaa ggggtagagc
    73621 cccattcaag agtatggaag catctggatg ttttttgagt tcttttaaaa agttttcttt
    73681 gttttttggt tggatcattt cagcaacagt catactaaga tagcagcgtt ccctgaatgt
    73741 ccctaaatat ttatgctgtt cttcagggtt cactaaaggt gtaccataaa gtcccttatc
    73801 caaatggctt tttacatcat cttgtgccat aaaaaatacc ctccttcttc tctttgtctt
    73861 tcttacgatt atagcatatt cccaataata cctaaaacat atgaaaaagc ggttgctttt
    73921 gttcaacgat tcacttaaaa taagaatgaa aatcattctc aattagaaag gtggattgga
    73981 tgcgctacgg acgactagta gcattattag ttattttaag tagtatttca ttgttcatcg
    74041 gagtatcgaa tgtacaattg tcagaactgc tccagttaga tgggcaaagc ttacaaatct
    74101 tgctagtgac tcgtttgcca cgaacgattt gtttgatcct tgtgggagcg accagtagta
    74161 tttgtgggtt gatcatgcaa catttgaccc aaaataaatt tgtttcacca acgactgcgg
    74221 gcacgatgga tagtgcgcgt ttaggtattt tagtggctat gattttccta cctggtgcat
    74281 ccttgattgt ccgatccttg acagcatttt gctttgcttt tgcaggaaca ttgctttttt
    74341 tatcccttac gcgattgttc ctccagaaaa atcaattgat gcttccgttg attggagtca
    74401 tgttagggaa tatcattggt tcagttgcta ctttttttgc ttatcaattc cagttggtac
    74461 aaaatatgac ttcatggctt caagggaatt ttgcgactgt gatacgagga aattatgagt
    74521 tattgtatgc tgttgttcca ttgttgctct tggtttggct atttgcttac cgatttacga
    74581 tcattggtct gggcgaatcg tttgcagtaa atctgggaat agattaccag aagatgcagt
    74641 ttttaggcat tggcttagta tcattggcta gtgcagtcat gttgatcatg gtaggaagca
    74701 ttccttttat cggagtgatc attccgaatc ttgtctctag aatatatgga gatcaagtgc
    74761 acaaaacaat tggcgtaact gctttagcag gtagtttgtt cttagtattt tgcgatcttg
    74821 tcgctcgcgt gttgattttt ccttatgaaa taccagtcag tgtgatcgtt ggaatcatcg
    74881 gtggggctat cttcctttat cttctgataa ggggacgacg atatgcttaa aaaaaagcca
    74941 tctattttta ttggtatgct tgttcttgtt ttatttgtta caggaatgta cttgacgatc
    75001 catacatatg gaaactggca ttttgcatgt gcattgagga gtaagaaaat ttttgcattt
    75061 atccttgtct cgctagctgt cagcagtgcc acaatcagct ttcagacaat tacgcataac
    75121 cagtttttga ctccttcagt attgggactt gagtcgttat atgtccttgt ccaaacggtc
    75181 cttttcttct ttatcggagg agtgacgatg cttcagcaga caggtacagt ttttttctta
    75241 ttgaacgttg ggctgatggt cgggctgagt ctattgttat ttttctttct tttgaaaaaa
    75301 atgagagcga atcttttttt attactgatg tcagggatga ttttaggtac gctttttggt
    75361 agtgtcagta cctttttaca agtcatcatg gatccgaatg aatatgattt gcttcaaggc
    75421 agattatttg caagctttgg caatatccag agtcagtatc ttttgcctgc ttctttgatc
    75481 attatcggct gctggtacct gttatttcga tatcgacatg tgctagacgt tttccatctc
    75541 ggaaatgaac aagcagtaaa cctcgggata gacacagatc gttttgaatt tttgatttta
    75601 tgttgtgtca gtcttttgac agggacatca acggctcttg tgggtccagt tactttttta
    75661 gggtttattg tcgcaaatat cagttatcgt gtcttttcta cttatcgtca tggtcagcta
    75721 tttatcggaa gctttctggt agctatgctg tttctgacag gaggacagct cgcagtagag
    75781 cagattttcg gctggaatac aacagtgagt gtcgtcgttg aatttgtcgg aggaatttat
    75841 tttgtcacaa aattgattcg cgaaaggaag agtatcctat gattgattta caaaaggtca
    75901 ataaactgta ccatggaaaa tcagtggtta aagatgtaca gttaacgatt cctgaaaaga
    75961 aattgactgc ttttatcggt ccaaatggtg ctggaaagtc aacggtgttg tcgatgatca
    76021 gtcgtttgat accaaaagat acaggtgaaa tctatttaga ccataacgaa gtaaaggctt
    76081 ggaaatccaa tgaattagcc aaaaaattat cgatcctacg acaatcgaat gaaataaatg
    76141 tccaaatcac cgtgaaagaa ttagtagaat ttggacgatt tccttatacg aaaggaaaat
    76201 tgacgacaaa agacgaggaa atcgttcagg aatcactgga acaccttggg ttagaagaaa
    76261 tagcggatca atcgattgac actctttcag gcggtcaact ccaacgtgcg tatattgcaa
    76321 tggtgctagc gcaagacacg gattatattt tactggatga acctttgaac aatctagata
    76381 tgaattttgc ggttcaaatc atgcagattt taaaaaattt agtacaagaa atcgggaaaa
    76441 caattgttat tgttcttcat gatatcaact ttgctgcaag ttatgcagac gaaattgttg
    76501 caatgaaaga tggacagtta tttacgcaag gaacgaccca agaaatgata aaaaaagatg
    76561 tattggatga attatacgaa atggacatac ggatttgtga gattgaaggc cgcagattct
    76621 gtctttattt ctaaaactaa cattaggaga gaagagatga aaaaattagt agtaggtatc
    76681 ttgattctag catcatttgg tttagcagca tgtggaaata gcaacaacac cagtcaggca
    76741 gacacaaaaa aaagcagcac acaaactgaa actacattga cgataacaga tagtaatgga
    76801 gaccaaatcg aggtgccaaa taatccagaa aaagtcgtcg tattcgacaa tggttcttta
    76861 gatacgatgg atgctcttgg tgttggagat aaggtagttg gagcagctac aagcagcctt
    76921 cctgagtatc tttcatccta taaaaaagta gaatcagcag gtggaataaa agaaccagac
    76981 ttagaaaaaa ttaatcagct tcagccagat ctaatcatca tttctggccg acaaagagat
    77041 tttcaaaaag atctaagtca aattgctccg accattttcc tttctttaga tgcgaaaaat
    77101 ccatgggaat ctttccagca aaatgtcact gctttagggg agatctttgg taagcaagaa
    77161 gaagctaaaa ctcaattaga ggaattatct agtgcaatcg atcagacgaa aaagaaagca
    77221 gaagctactg acaaaaaagc actagtcaca cttgtgaatg aagggcaatt atcagcctac
    77281 ggatctggtt ctagattcgg tttcatccat gatttatttg gctttgaaca agcagatgat
    77341 caaatcgaag catccactca cggtcaaagt gtttcttatg aatatgtgct ggaaaaaaat
    77401 ccggatatct tatttgtagt agacagaaca aaagctattg gtggagacga cagcaaagat
    77461 gatatttctg ccaatgaact ggtagcacaa acgaatgctg gtaaaaatca gcagatcatc
    77521 tcccttgaac cagatgtctg gtatttaagc gggggcggtt tagaatcaat gaaactgatg
    77581 atcgaagatg tgaatcaagc atttaaatag cataaataac agaggattaa actctcatac
    77641 tatgtaacaa taatgtttct tacattctta aatagcaaga gcctatcctc aaagaagtta
    77701 tcaaaaacca tccgtaaaat cgtattgatg attttacgga tggtttttat ttatttgaga
    77761 aatctttttt caacgtgaat tcattatcta catcatacgg gttgattcta gaatggggag
    77821 tggcaaaaaa tagtgtgatc cattttttca gcatcaagat cgcctccttt gatttgaatc
    77881 aaaggtacaa agcgaaactt agataaaact taaatttaga tatttttatt ttgtaccgat
    77941 ctcttcttta aacgatgttc catctgctgc ttctcggcaa taagtccact agactagaaa
    78001 atatgagaag ctattttctg tccagcgttc ttattactca aagcaaatcg cagttgtcac
    78061 aatctcttct aaacgatgtt ccatctgctg cttctcggca ataagcccac tagactagaa
    78121 aatatgagaa gctattttct gtccagcgtt cttattactc aaagcaaatc gcagttgtca
    78181 caatctcttc taaacaatgt tcaatcagct gaccttcggt attagctcaa agcactgatt
    78241 tcacaacctc tatttaggca tgtgtctcag caagcggttg atagttgatg atctgcattt
    78301 ttcgataaaa atcggcggga agcttactcc accgttttaa taattgatag aaataactct
    78361 cttctgcttt gattttatac tcgcttaata tcaatccaaa actattcgtt ttttttgaag
    78421 ctaatttttt ataaatatcc aatgctttgg ttttatcttc attgacaaag agtgttttta
    78481 cttcaatata agaaccctcg atattttgtg tttcttcgat aaaatagatg ttttttatat
    78541 attccttcat agtgattcct ccaaagaagt cttttatttg ataagtttac tctaccgaag
    78601 agttgttaat aatccgtgaa caaaaagtaa ttctttttcc aatagttgtt aaaggttttt
    78661 tattttttat agtatttttt gttatttaag aaaggaataa ggttaaacaa ataacaggtt
    78721 gctttttggg tctttttccg ttcaagaaac tgaacgaaga aaagggaaag ttgtctcttt
    78781 tgtgataaga gaaatcataa taaacgaatg acttactttc caataaattg agaacagtca
    78841 gaaccagaca aattagttgc atctgatgaa taaagtgatt attctatgac atgaggtgat
    78901 acagatgaaa ttagaagtga caaaagaagc acaagaagta ttgaagaata aattagaagc
    78961 agacgatcaa cttttattag atatcgaaaa cggtgacggt ccatttgcag aaagccaagt
    79021 cagttgccaa ttggatacgg catttcgatt gatcattgtc aaaaaagata cacaaacaga
    79081 tctaagtctt tattctgtag tggctgatac accagttgga ccaatcaaaa tcaaagacag
    79141 tgcaatactt tacatggatg atccatccac acttgcttta gaaccaactt ataatagttt
    79201 acaattaaaa ggaccaagcg gcttgagaaa aggcaacttg caagttgttc gaaaagacta
    79261 gagggataaa atgggaaaat tggcgatcat cagcgaccta catgtggaca tcaataaatt
    79321 cggcgaatca gagctgatgc agctttggca agttttgcaa aagcacaaga tcactcggct
    79381 tcatctagcc ggagatacgg ctaataaagt agaccgttgc ctagctgttg tcgaattttt
    79441 cgtcaataaa ggattatcta cgacatttca tttcggcaat cacgagttgg cagatgtgcc
    79501 caatgaacag atgatggacc attttccaga cccacatttt ttaaatgaac ggtatattgc
    79561 tttaaatgag cagaccgttc ttttagggat gaatggctgg tacgactatc agttttccga
    79621 attgaaagat tacgaggcaa tcgtacgaat gaagcgtttg tattggtatg atcggttcat
    79681 ccagcgtggg aaaacagatc tcgaggtcaa tgcagaaatg cttgcagctg caaaaaattt
    79741 gctagaccag ctggaagcaa aaaaacttga ggtgatttta tccactcatt tcgttcctaa
    79801 acgggcattt atcatgtatc aatctgctcc ttatgagcgt tggaacaaat taaatgcatt
    79861 tttaggatca gaatcattcg gagaattact tgaccgttat tcaaatatca aacaagtagt
    79921 ttttggccat acgcatcgtc gctttgaaga taaatccatc ggtcacacga tttacagctg
    79981 cagacctttt ggttattatt atgaatggca gctgacccga gattttgtct tgaaagaaca
    80041 gttgctagac agctatgatc ctatgaaatt acgtgtcctc ttgcgtgccc atcacccagc
    80101 ttttcaggcc tatcaaacac aacatttgca agaggaattt gaacaagcga tgacaatcgt
    80161 tccgtattga gacagcatta gtaatgtgta aaagaaaata ccttgtaaaa cataaggata
    80221 agtaagagca gtagatccat ttatcgtgga tatgctgctt tttttgcgga aaataatcag
    80281 cgaatgttta tttgatcgga tgatttgtaa ctcgcagagt actagatgca agcgtgtatt
    80341 ctttttttga acgataatca caaaaaaaaa tataatatac attttttatt taattataat
    80401 taatattgat tgaagtctag gcagtcaagt cacattttga tagataggag ggggacttac
    80461 gaattatttt aaaggctatg acagctgtat ttctagattg tgtttgcatc aagttgcaat
    80521 aaaaactaga aatcaaatcg ggctgataat catttataac taatcgatac ataggaccaa
    80581 atataggtct atgttctttt atttcataag tatcgtaaaa cagaattttg ctatggatta
    80641 atccaatcga ctgatatgtt tagtaaataa agaaagagga atgtcaatga tgaaaaaaga
    80701 aatcgtatta acagcagcaa tgatgttatt cagcatggta gcaagtacga cttttgtttc
    80761 tgcaactgaa gtatacccaa aggaatataa cacagaagga acgattacat ttgaagcagg
    80821 agatgagggt gtaactcctc ctgttgatcc agagaatcct gatcccaata aacctgtcga
    80881 tccatcagat cctccaagcc ctggaacagg aggggcatta tcgattgatt atggatcaaa
    80941 attcaaattt gggactcaaa aaatttctac tgcggataaa acgtattatg cagctgctga
    81001 tgtgatgaac gatgggtcta gaaaaccgac atatgtccaa gtgacagata ggagaagtac
    81061 cttatctggg tggaaattaa gtgtctctca accagaacag tttaaaactg ccagtgggga
    81121 tgaactagtt ggagcacagc tgaaatttac gaaagggcag gctgtttctt tggttgatcc
    81181 aacatatacg ccgcaaacgg tgaattctga attaacgttg acaccgggag gaaacaatac
    81241 gttagcaatt aatgctaaat caggaacagg tgtggggact tgggtctatc gatttggtgc
    81301 aaatgaaaat gaaaatcaag atgcggtaca gctttcggta cccggtaaat ctgtcaagtt
    81361 agcgcaacaa tattccacta aattagtttg gacattagaa gatacaccta acaactagtt
    81421 tactgaaaag aactgttaaa aataaatacg tagtcatatt gtcttgcgag gtaatcgagc
    81481 aaaaattgtc aaaggctagg ctaaatttgt ttggatacga atagttcatc taggaattta
    81541 acgatcagga ggaaaagata tattgaaaaa gaggaggatc gtgctcattt taagcagtct
    81601 cttagtgact aatatctttc cgatcatagg gtactctttg gaattgaatc agttggagca
    81661 tggaaataga agagacgaag aaagcaaact cacttcgaca ttcgacagtt taatagaatc
    81721 caaccagaca caagaaagca gtgaggattt aagtatttcg agtagtggag agaccactga
    81781 caacgtagaa gctgagggtt cggacaaaca aggactagaa gatgcaccaa tttctgcggt
    81841 tgagctgcct gacgatccca atattatttc tatcgataaa atttttcaaa cacctatcgg
    81901 tactagcacg tccatattgg aaaatgggaa gttactgcag ttgaacccag cagaaaggtc
    81961 tcaaagagga gctatttggt caaataagaa gattagttta ctctcggatt ttacatttaa
    82021 atcttattta tacttaggaa atcaacgaga tagagcggga gatggcatga cctttacatt
    82081 gactggcgat cctagaatgg ctagtaatcc cagtcaagtt attggatcgc caggtatggg
    82141 aattggctca tattctactc gttctggcca accttacatt gcgaatgcgc tctcgattga
    82201 gtttgatacg tataaaaata gtggttcttc tgatcgaatg gatcgagaga taactgctga
    82261 taatagaggg catggacatg tcgcttttgt cacgcctaaa ccaaataata ataattattc
    82321 tggagagcat actggcgtga cggtagctcc tacatttctt tctaatggca catggcgctt
    82381 gttaataatt aaatgggacg cggagcaaag aagtcttact tatgatttgg aaggtgtcgg
    82441 tcactcttct cggattatac ctgatctcat taggcagttt ggttctacag aagtaaattg
    82501 ggggtttact tcttcaacag gtactttttt tcaagaaaat gctgtcgctg tcacgcgact
    82561 tccatcgaat gtcacatcaa cagctgatgt aaaagtcaac gatagtgatt atggggccat
    82621 cgctgaagct ggaggaaacg ataagataac tatcagaaat acattgaagg tcgaggggga
    82681 ttttcttgaa tataagaaaa tgcagactag tatcgacctc ccacaagaat taactattcc
    82741 cgagcatatc ttgatcaatg gaaatcaaat tgatgccaaa gatatcttga taaccggaaa
    82801 tcgagtcacg ctaaatctga cagattattt tattgctgga gatacgttaa taatcgaatt
    82861 ggagacaact ttgcaggaag taattcctga aaaagtactc actatgcatt ttgaatacct
    82921 tgaaaacggg aatttgattg agcaatcgaa tgaaataaac ctaaagatcg caaagctgaa
    82981 ggagatagat atccatgttt attataaaga taaagacagt cagattgata ttgctgaaat
    83041 gaaaacaatc actgggaaaa taggagagaa gtataaagaa actcctattg atattgaagg
    83101 gtatgttttt gactcggatt cagcgaatac agaaggcatc ttctccgaaa atacagagga
    83161 tatctatttt tattacagaa aaggcaaact ttatctctca actgctcctc aatatgtcaa
    83221 ttttggaaaa cataaaatat ccagtagacc attaagtgaa ttcggacagt tttcaggtgg
    83281 gttgaaaatt atagatgaac gggcttttgg aagttggaaa ttacaattga aacaagagaa
    83341 agttcttaca aatggagatt ttgagcttcc agaagctctt tcctttgtta cctctgaaaa
    83401 tacaacggtt atagggacat ccgctgttac gattttcgaa agtgatcaaa agggtgaaag
    83461 cgatttgtcc tctcttttag attctacagc tcatcgtgga atcagaatag atattcctgt
    83521 agaaaaccaa agaatcggaa cctttgaggg gaaactatct tggatacttg cagatgtccc
    83581 aggaaattaa caggaagaga agaatataac aaaattattg agtttcaaga agggcacagg
    83641 taccataggc tcttgtgtcc cacagagaca agattttgtt atagtcttct cttacatatg
    83701 attaaaggag cgattttctt gagaccaaaa caacaattat ttataacact cctagttatt
    83761 ttaggggcac tttttaacaa tatatcagtg tcagctagtg catttaattt ttcagtgacg
    83821 ccgataactt cagagaatca aatcgacaag agaaaaactt acttcgatct tcaacttgat
    83881 ccggatcagg aagtagaagt aaaagcggaa ttgagaaatg atacagaaaa agaagtgaaa
    83941 atcgatattt ctgtgaatag cgcaaccacc aattcaaatg tgatggttga atacggaaaa
    84001 aacgagattg aaaaagacat gagcctgatc tttgatctgg tagattatgt gagctatcca
    84061 cagatagtga cgctaaaacc taaaagtgtc caaaccgttg cttttcatgt caggatgccg
    84121 aatgagcgat ttgatggtgt attggctggt gggatcacat tccaagaaat ccagacggaa
    84181 aaggaccaaa cagaaactaa ggatcaaagc ttatcgattg agaatgaata cacctatatt
    84241 attgctttgt tgatgcaaca gacactgaac gaagttgctc cgaaccttac tcttcatgaa
    84301 gttaaaccag atcaaatcaa tgcaagaaat gtcatcttag caaacgttca aaatgatcaa
    84361 aaaacctata tcaatcaagt agttattgaa acaaaaatta caaaaaaagg acattctgaa
    84421 gttttatatc aagaggaaaa agaaggattg cagattgcgc cgaatacaaa tttttctttt
    84481 ccaactgctt taaatggtca accgctaacc ccaggtgaat atcaccttac aatgactgtt
    84541 ttaggaaatg aaaatgaaaa tggaaaattt tcgagaaaaa aaggaaatac tacgatcaac
    84601 tataccaatc aatgggtatt tgaaaaagat tttacaatag acggaaaagt agccaaggaa
    84661 ttgaacacga cggatgttac gattaaaaaa gacaataatt ggatttatat cttagtagct
    84721 tcacttttct tattgtttat tttattatgc atacttttgt ttgttttgag aaaaaaagaa
    84781 gagaaagaaa agtaaggtta agataacaaa tagtaaattt ttattattgt aggatggttg
    84841 ggctcttttt ataacaatat aaaatatacc attttattaa taaaaatgca tactagtaaa
    84901 tgatataatt ctaataacag gtttgatcgc gagaaaggcc aaataaaacg ctctccaaaa
    84961 tgttaaaaaa aagacaggac gatgtatatt atacaaacaa gaatcgctga caacagtgac
    85021 tcggatccag gaaatcagtt ttatccccag ctagttggtg aatgcatgga aagtaatata
    85081 cgtagaaaga gctgatgact aatgaattat gagttttttt taagcaaaga agatcacaga
    85141 agttattcat tacttaaata tttagaagca agctcaaatt tatcagagtc aatatcgaat
    85201 gttcaagaag agttgtcgct atcgacattt ctcttgaaaa aaacgatcga taagctgaaa
    85261 catgatttac aaaagttcga tctagagaaa aatttgcgag taaccgtcag tgaattggat
    85321 atatgtctgg aaattaatgg aaaatgttct agcaaagctc tgttatctcg ttatataacg
    85381 gattctttat ctatgaaaat ggtattggct ttctttcagg aaaaatatat ctcagtagaa
    85441 aattttgcta aacagcatca cgtgagttat tctgtggctt ataaagtatt acaaagacta
    85501 aaaagaaatc taaaaaaata tcaaattttt tttgaaaaga gaaagttga
//
LOCUS       DACDNO010000004        80429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDNO010000004 DACDNO010000000
VERSION     DACDNO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.004"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(717..3866)
                     /locus_tag="IWI22_000272"
     CDS             complement(717..3866)
                     /locus_tag="IWI22_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9167697.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3879..5078)
                     /locus_tag="IWI22_000273"
     CDS             complement(3879..5078)
                     /locus_tag="IWI22_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9167698.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5068..6663)
                     /locus_tag="IWI22_000274"
     CDS             complement(5068..6663)
                     /locus_tag="IWI22_000274"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9167699.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(7247..7954)
                     /locus_tag="IWI22_000275"
     CDS             complement(7247..7954)
                     /locus_tag="IWI22_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167700.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(8019..8855)
                     /locus_tag="IWI22_000276"
     CDS             complement(8019..8855)
                     /locus_tag="IWI22_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9167701.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8873..10282)
                     /locus_tag="IWI22_000277"
     CDS             complement(8873..10282)
                     /locus_tag="IWI22_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9167702.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10296..12119)
                     /locus_tag="IWI22_000278"
     CDS             complement(10296..12119)
                     /locus_tag="IWI22_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9167703.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12962..13288)
                     /locus_tag="IWI22_000279"
     CDS             complement(12962..13288)
                     /locus_tag="IWI22_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167704.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13334..13696)
                     /locus_tag="IWI22_000280"
     CDS             complement(13334..13696)
                     /locus_tag="IWI22_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9167705.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13733..13948)
                     /locus_tag="IWI22_000281"
     CDS             complement(13733..13948)
                     /locus_tag="IWI22_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9167706.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            14075..14410
                     /locus_tag="IWI22_000282"
     CDS             14075..14410
                     /locus_tag="IWI22_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9167707.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14486..16483)
                     /locus_tag="IWI22_000283"
     CDS             complement(14486..16483)
                     /locus_tag="IWI22_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167708.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16581..16856)
                     /locus_tag="IWI22_000284"
     CDS             complement(16581..16856)
                     /locus_tag="IWI22_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9167709.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            17088..17822
                     /locus_tag="IWI22_000285"
     CDS             17088..17822
                     /locus_tag="IWI22_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9167710.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17861..19003
                     /locus_tag="IWI22_000286"
     CDS             17861..19003
                     /locus_tag="IWI22_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9167711.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(19098..20489)
                     /gene="sufB"
                     /locus_tag="IWI22_000287"
     CDS             complement(19098..20489)
                     /gene="sufB"
                     /locus_tag="IWI22_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9167712.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(20494..20964)
                     /locus_tag="IWI22_000288"
     CDS             complement(20494..20964)
                     /locus_tag="IWI22_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9167713.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(20951..22186)
                     /locus_tag="IWI22_000289"
     CDS             complement(20951..22186)
                     /locus_tag="IWI22_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9167714.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(22183..23469)
                     /gene="sufD"
                     /locus_tag="IWI22_000290"
     CDS             complement(22183..23469)
                     /gene="sufD"
                     /locus_tag="IWI22_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9167715.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(23482..24255)
                     /gene="sufC"
                     /locus_tag="IWI22_000291"
     CDS             complement(23482..24255)
                     /gene="sufC"
                     /locus_tag="IWI22_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9167716.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(24452..25300)
                     /locus_tag="IWI22_000292"
     CDS             complement(24452..25300)
                     /locus_tag="IWI22_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9167717.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(25324..26010)
                     /locus_tag="IWI22_000293"
     CDS             complement(25324..26010)
                     /locus_tag="IWI22_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167718.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(26003..27037)
                     /locus_tag="IWI22_000294"
     CDS             complement(26003..27037)
                     /locus_tag="IWI22_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9167719.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(27432..27770)
                     /locus_tag="IWI22_000295"
     CDS             complement(27432..27770)
                     /locus_tag="IWI22_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9167720.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(27772..28128)
                     /locus_tag="IWI22_000296"
     CDS             complement(27772..28128)
                     /locus_tag="IWI22_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9167721.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(28134..29321)
                     /locus_tag="IWI22_000297"
     CDS             complement(28134..29321)
                     /locus_tag="IWI22_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9167722.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(29512..30357)
                     /locus_tag="IWI22_000298"
     CDS             complement(29512..30357)
                     /locus_tag="IWI22_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9167723.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            30591..31556
                     /locus_tag="IWI22_000299"
     CDS             30591..31556
                     /locus_tag="IWI22_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9167724.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            31933..32976
                     /locus_tag="IWI22_000300"
     CDS             31933..32976
                     /locus_tag="IWI22_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9167725.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(33038..33502)
                     /locus_tag="IWI22_000301"
     CDS             complement(33038..33502)
                     /locus_tag="IWI22_000301"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9167726.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            33748..34695
                     /locus_tag="IWI22_000302"
     CDS             33748..34695
                     /locus_tag="IWI22_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9167727.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(34906..36978)
                     /locus_tag="IWI22_000303"
     CDS             complement(34906..36978)
                     /locus_tag="IWI22_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9167728.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(36911..37288)
                     /locus_tag="IWI22_000304"
     CDS             complement(36911..37288)
                     /locus_tag="IWI22_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9167729.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(37417..37977)
                     /locus_tag="IWI22_000305"
     CDS             complement(37417..37977)
                     /locus_tag="IWI22_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9167730.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            38127..39419
                     /locus_tag="IWI22_000306"
                     /pseudo
     CDS             38127..39419
                     /locus_tag="IWI22_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
     gene            complement(39479..40597)
                     /locus_tag="IWI22_000307"
     CDS             complement(39479..40597)
                     /locus_tag="IWI22_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9167731.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(40611..42032)
                     /gene="arcD"
                     /locus_tag="IWI22_000308"
     CDS             complement(40611..42032)
                     /gene="arcD"
                     /locus_tag="IWI22_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9167732.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(42278..43399)
                     /gene="mnmA"
                     /locus_tag="IWI22_000309"
     CDS             complement(42278..43399)
                     /gene="mnmA"
                     /locus_tag="IWI22_000309"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9167733.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            43803..44402
                     /locus_tag="IWI22_000310"
     CDS             43803..44402
                     /locus_tag="IWI22_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9167734.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(44540..45826)
                     /gene="efmA"
                     /locus_tag="IWI22_000311"
     CDS             complement(44540..45826)
                     /gene="efmA"
                     /locus_tag="IWI22_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9167735.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(46097..46441)
                     /locus_tag="IWI22_000312"
     CDS             complement(46097..46441)
                     /locus_tag="IWI22_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9167736.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(46578..47714)
                     /locus_tag="IWI22_000313"
     CDS             complement(46578..47714)
                     /locus_tag="IWI22_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9167737.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(47903..48877)
                     /locus_tag="IWI22_000314"
     CDS             complement(47903..48877)
                     /locus_tag="IWI22_000314"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9167738.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(48957..49832)
                     /locus_tag="IWI22_000315"
     CDS             complement(48957..49832)
                     /locus_tag="IWI22_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9167739.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            49962..51869
                     /locus_tag="IWI22_000316"
     CDS             49962..51869
                     /locus_tag="IWI22_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9167740.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(52410..55688)
                     /locus_tag="IWI22_000317"
     CDS             complement(52410..55688)
                     /locus_tag="IWI22_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9167741.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(55959..56504)
                     /locus_tag="IWI22_000318"
     CDS             complement(55959..56504)
                     /locus_tag="IWI22_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9167742.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(56516..57208)
                     /locus_tag="IWI22_000319"
     CDS             complement(56516..57208)
                     /locus_tag="IWI22_000319"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9167743.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(57227..57511)
                     /locus_tag="IWI22_000320"
     CDS             complement(57227..57511)
                     /locus_tag="IWI22_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167744.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(57524..58087)
                     /locus_tag="IWI22_000321"
     CDS             complement(57524..58087)
                     /locus_tag="IWI22_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9167745.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            58315..58977
                     /locus_tag="IWI22_000322"
     CDS             58315..58977
                     /locus_tag="IWI22_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9167746.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            59101..60297
                     /locus_tag="IWI22_000323"
     CDS             59101..60297
                     /locus_tag="IWI22_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9167747.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(60398..61774)
                     /locus_tag="IWI22_000324"
     CDS             complement(60398..61774)
                     /locus_tag="IWI22_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9167748.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(61811..62677)
                     /locus_tag="IWI22_000325"
     CDS             complement(61811..62677)
                     /locus_tag="IWI22_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9167749.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(62766..63791)
                     /locus_tag="IWI22_000326"
     CDS             complement(62766..63791)
                     /locus_tag="IWI22_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9167750.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(63809..64483)
                     /locus_tag="IWI22_000327"
     CDS             complement(63809..64483)
                     /locus_tag="IWI22_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9167751.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(64667..65560)
                     /locus_tag="IWI22_000328"
     CDS             complement(64667..65560)
                     /locus_tag="IWI22_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9167752.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(65634..67331)
                     /locus_tag="IWI22_000329"
     CDS             complement(65634..67331)
                     /locus_tag="IWI22_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9167753.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(67324..68637)
                     /locus_tag="IWI22_000330"
     CDS             complement(67324..68637)
                     /locus_tag="IWI22_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9167754.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(68637..72674)
                     /locus_tag="IWI22_000331"
     CDS             complement(68637..72674)
                     /locus_tag="IWI22_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9167755.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(72687..73145)
                     /locus_tag="IWI22_000332"
     CDS             complement(72687..73145)
                     /locus_tag="IWI22_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9167756.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(73443..73961)
                     /locus_tag="IWI22_000333"
     CDS             complement(73443..73961)
                     /locus_tag="IWI22_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9167757.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(73963..74580)
                     /locus_tag="IWI22_000334"
     CDS             complement(73963..74580)
                     /locus_tag="IWI22_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9167758.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(74577..75431)
                     /locus_tag="IWI22_000335"
     CDS             complement(74577..75431)
                     /locus_tag="IWI22_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9167759.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(75428..76339)
                     /locus_tag="IWI22_000336"
     CDS             complement(75428..76339)
                     /locus_tag="IWI22_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9167760.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(76380..77714)
                     /locus_tag="IWI22_000337"
     CDS             complement(76380..77714)
                     /locus_tag="IWI22_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9167761.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            77922..78752
                     /locus_tag="IWI22_000338"
     CDS             77922..78752
                     /locus_tag="IWI22_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP9167762.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(78996..79245)
                     /locus_tag="IWI22_000339"
                     /pseudo
     CDS             complement(78996..79245)
                     /locus_tag="IWI22_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(79436..79630)
                     /locus_tag="IWI22_000340"
     CDS             complement(79436..79630)
                     /locus_tag="IWI22_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167763.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(80135..80320)
                     /locus_tag="IWI22_000341"
     CDS             complement(80135..80320)
                     /locus_tag="IWI22_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167764.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT        23253 a        17027 c        13994 g        26155 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttgggcac ttgaatctcg gctattttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcgtccttca cgaatgagct gattgggaaa ctcttttgtt
     2041 aaccaatcag gctctttcgt catctctttg atggcccgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat tgcagatttc gttggcctgc catttcgaaa atcaaatttt
     2161 gtataggcat tatctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag ctgggtccaa ataactttct tttttgacag gttccatttg atcaatcata
     2281 cgattgatct gttctgatga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctagtgaagt cggctccacg gtgttttcat gctctacttg aaatcctaag
     2401 acagagccat cttctagcgc attcttaatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggttg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg aaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg gcccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgtttct ttgcatattt caacgccgca
     2701 tctagttttt gacgggttgt aatgattaca acatttgcat tggcatctgc caacagggtt
     2761 tcacttaatt ctttggcact tcccgttcca acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aattttgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gttcgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtc tttcctgaac cagtcgcatg ccacacatac
     3001 cctgattgat gtttatttgc ggcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgagggtttt attatcttga tcttcactga cgattgtata atccgcaatc
     3121 aaacgatgag catccgggat atttaacact tgtttacaaa actcatataa gttttctact
     3181 ttttcattgt ctttcgttcg ccaactaaat aaaaacttag ggtgcaagtc tttcggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaatgcctga
     3361 taaacaccat cttttgcagt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 attaataagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata agtagagatt cctccaccaa tgtcttgatt ggaataaagg
     3541 ataagtgaga cagaacccaa ttgcgaatct tcccgttcaa tcgtgatccg agccatccca
     3601 ttttcacctt ttaaccattt cgcagcatca aatggggttt tcgttcgcag cagtaattcc
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagataa ttttttggcg taaattgacc caaaggtctt cttctgattt tagatccgga
     3781 cgatacgtcc actgattatg cccttctcca agtacacgaa tcaactgttc ttcgacttct
     3841 gcttcctcac ggtgactaat tttcattctt cttccctcct agacaaacat cttttgtaag
     3901 aagctttttt tcgtttcttt tagtaaatct aacttacgtt gatgaagagc gatcgtatca
     3961 tctagctgtt tgaagaaagt accgattttt tgttgttcat cctttacagg aaccttaaca
     4021 ctgatattct caatatcaga actgttataa gaaggcaatg cccctacttg ggagtatctt
     4081 ggcaaattta tagtttcaaa caatatttta ccaaaataga tatcccaagt attattaaat
     4141 aaataagcca tggtattgtt atctattaag aaagggcttg tgaccaaacg ttttctatta
     4201 agcattattg ctgcgccaac tttagcaaat ataatcgctg gcacatcttt tattggcttc
     4261 cattttttac gctcaatttg ctcattagat acataatgat ttgcatttct catttcatac
     4321 tcattaccaa tgttattcat gtcggaaact ttaaaaaatg gagtaccttc agaaccacct
     4381 tgctcggcat caggaaatcc aattcccgat tgagttttac ctaattctcc caacttacgc
     4441 tcttcccaat cttccgtaaa accaggaaac cggatttctg gcacttttgc tccgttttca
     4501 gggaacattt tttgtaagaa tccttttttc gtttccttca gcaaatctaa cttacgttga
     4561 tgaagagcga tagtgtcgtc gagttttttg aagaatatgc cgattttttg ttgttctttt
     4621 aaatcattgg gaatatagaa tactgtatct gaaacttttt tccagtctga acgaggcatt
     4681 tttgttccag atgtatcatt tgcagccttt tgatatctat cagcttgaat aagagaataa
     4741 ataaagtccg agtcgctctt cttagctcta aaaacccaga aatccccaat cgctacgcct
     4801 gtaaaagttg ccttgagcca attttttaaa tatggacgta actttccata tagaatatca
     4861 tttggtaaaa attcaatccc ttttctatta tcaaatttct ggctcacatc tttattcagt
     4921 ctaccttctc ctgccacgat atcttcaaat tcaacttttg gaagtctact ggagtttgta
     4981 cttttattaa gtctaacaac taaatcaatt aacttacgct cttcccaatc ttccgtaaat
     5041 cctgggaaac ggatttctgg tcgtgtatca ttgctcatgt ttgaacacct ccagcgcacc
     5101 ttgtaaccac atttcgtttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc
     5161 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     5221 atcaattggt tcttcttctt cgaaagtatc tacatagcgt gggatattta agttatagtc
     5281 attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc
     5341 agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat agtttatttt gatttttccc
     5401 tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt
     5461 caacacaatc accgtcgttg gaatcgatgt cccaaagaac aagttcgcag gcataccgat
     5521 cacagcataa atactgccat cttctagtaa tttttgacgg atgacccctt ctgcagcgcc
     5581 acggaataaa actccgtgtg gtaagacgat ggccatggta cctgtttcct tcaaatgata
     5641 gaacccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt tcccataacg
     5701 attaaaccga gaatcatcta agaaagtcgt atctgctgac caattagccg aataaggtgg
     5761 gttcattact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt
     5821 atccccattg cggagattca tttcttcggc gtcgacacca tgtaaaatca agttcatttt
     5881 tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctgggtg
     5941 tgttaagtag ttacgtacgt ttaacatcaa tgaacctgaa cccatcgttg gatcaaaaac
     6001 actaaagaat cgtcgttcct tttgatcgag cgttacgatt tgcgccatca tatcagaaac
     6061 catatgaggt gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     6121 cagaaactca tacgcatccc cgatcacgtc cccatcgtgt tctaaaacat ctacatcatt
     6181 caatttcttg atgacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     6241 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     6301 ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc
     6361 taagacgttg aaaagatact ctggtgcaat tgcatatccc aaaatatcaa cgattgtggc
     6421 aatcagatct tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt
     6481 gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     6541 tcgatcggat aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc
     6601 atccatttta ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
     6661 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
     6721 ccccctttta taaaaagcaa aaaaaaaaga aagaaactta ttcttgctaa taaactacgc
     6781 gaaaggagtc taacgttgtt tgatataact tagactgatc gttaacttat tttttatatt
     6841 tctacatagt tatctttaga accatttcag atacatatga aacaattaca tatgattttt
     6901 tcataaaaaa acagtactgt ctagtagtat aaaatataca aatttcttaa aaattttttc
     6961 actaaaatca ctacctgaaa cagtctaaaa cacttatata tcaacgttta atgaaactat
     7021 ctccgtttca tttcagatac aaataaatat agtcgggctc caaatccgaa aaaaaatacc
     7081 cccagtagct tatcctaagc tactggagat gtataagtac ggtcatataa caacggggta
     7141 gggtaaataa atgaaaaaga tggaagaatc attattcaat aactttttta aatacttcgt
     7201 tgtaatcagt tacctgtccg ttcttaaata ccacagaata atctttttac ccattttcaa
     7261 gtaaccgaag tggaatgtct ttaccaactt gctctgtcac tgcattactg atacctgtta
     7321 gattagctaa taaatctgat aaagattgag catctgtaga aatctctgta tcagtattta
     7381 tcgtgattcc tttatcttca tttacaagct tccaagtagg tggtatagct gtgtatgttt
     7441 cgatgaatga atcaattctt tcatctatcg tcttcccagt atctacttct gtctcattac
     7501 tcttagatga atcgattctt tcatccatcg tctgctccgt aactgcttct gtctcattat
     7561 tctcaccgga aactctcttc acacccaaac ttatatattg tccatcttta gtgtgcatag
     7621 ttaaaattcc cacgctttta ctaataaatt cactataacc agttctttca atagagactt
     7681 caatgtattt cacactatca tactcaaaag aaaaatagac atgtaaaagc attttgttgt
     7741 ttacatctac ataataatcc tttgactcta cttgagacct tcctatacgg aatacagctt
     7801 tactgtctga tatctctata ccagcatcct tattattaat ctgccaatct ccaagtagct
     7861 cttctggcaa gctagtagtt tcttgtgaac actttaccac ttcactggta gaggtcgaaa
     7921 cattcacctc cgcactgtta gaagaatttt ccaaggaatc aggagcctta gcacaaccaa
     7981 caagtcttta tatattgtgc agccgatatt ttcttcattc agtacaccct tgaaatatgc
     8041 agtgttagat aaagcagttc gtcctgattt gctgaggcac ccatctcttc ttgaacgtac
     8101 tccttgattt tcaaggccgc ccgatactct ttcggaaaac gctgcttcac tgaagccagc
     8161 aaaaaatcat ccttctccgg caactcttcc ttcttaatca ttcgggcaat aaaaaactgt
     8221 aaatgcgtca caaatcttaa gtaaagcatc gaatctttat caaccttttt ttcgggaaac
     8281 agatatccta gcatagccaa gatatccttt agtgctctta ccgagagcat ggtttcttcc
     8341 atatttgctt gttcactttg ggcatttaca atgtgaaacg caatgtttcc agcctcctct
     8401 tctggcaact gaacattcat ttttttgttg atcaattgga tagcatagca gccgatctca
     8461 aattcttttg ggtaatactt ttgaatttca atcagcaggc gattttgaaa aacaatgcct
     8521 ttttgactgc gttcaatggc atgcttcaag tgatcaacca aggtaaaaaa tagactttta
     8581 ttcagcttaa agtcaaactt ggcgttggca taatcgataa ttcgcttgac gcaaataatt
     8641 agatcatccg gagcagcctc aaacagttta atgtaatcct tttttagagc atcagaaagt
     8701 gaatctaatt taaatacttt ttggattttg tcagccggaa ccaaatcgcc tttctttagt
     8761 tgaaatccaa tccctttccc catcaaaatt acttctttgc cttgcttatc taagcaaaga
     8821 attaaactgt tgtttaacgt tttaattact ctcattagat tcaccgttct ctctaagcta
     8881 agccatttct tgcgattact ttttgatacc aaaagaagct ttcttttttt attcgtttta
     8941 attctttaag attctcatcg gttcggtcaa catatataaa accataacgc ttattgaagc
     9001 cttgatgggt actaacaaca tcaatcgctg accaaggtaa ataaccaaaa acatctacac
     9061 catctgttaa agctgcttga atagccagaa tatgtttggc tagataatca attcgatcct
     9121 ggtcattaat tcgtctatct tcctcaacta tgtcgggtgc cccatagcca ttttcagtaa
     9181 ttaaaatagg taaatcatag cgatcgtaca atttcctcaa tgttaatcgt agtccagtag
     9241 ggtcaactac ccagccatat ggcgttttat ctacataagg attttcagct gcccggtaga
     9301 caccttcttc tcccaacatg atctgttgat cccccgctct agcggaaaca tctgatccgt
     9361 catttttgct ggcagcgatg gtggcagttg aataataatt aatcgcaata aaatcgggtt
     9421 taccggagga taaaattgcc tgatcctctg cctcaatttt aggatataat ccacgatcat
     9481 tcaaatagtt ttcaaacaaa taattgtatt ttccccgaac tgctacatct aagaaagacc
     9541 agcctctcaa tgtttcccaa ttatgggctg caattgcatc ttctggactg gaggtagctt
     9601 gatacatagc cgttaaattg attgctggac cgatttttcc tttaagattc aactgatgat
     9661 acaagtgaat tactctagct tgagctaata aaacatggtg attaatttga aagagttctt
     9721 tttttgaagg cagctctcca cttggtggca aaccaatcgc tccaggatga aggatcatcg
     9781 tgttttgctc attaattgtc agccaatagt tcaccttgtc accatactca gtaagcaata
     9841 cctgtacata gtccacaaaa taatcgatag ttgctcgatt taaccaaccg ccctgctcct
     9901 ctagaaaata aggtaaatcg aaatggtaca tggtgacaat cggttcgatt ccctgattca
     9961 ggcattcatc aattacttga tgataaaaag aaattccctt ttgattggtt ttattatttt
    10021 caatatccat aattcgcgtc caagcgatgg aaaagcgaaa agcttttaag cctaattctt
    10081 taaacagctg tatatcttct ttatagtgat ggtaaaaatc cgaggcaact tcaaaattag
    10141 ctaccccttc tgtatgctga tacttgtcaa taatagacgg ttgtttccca tcctcctgat
    10201 aggcaccctc tgcctggtag gcactaatgg aggcacccca taaaaacttc tctggaaatg
    10261 ctgaaagctg tttgtattgc acaaaaatcc tctccttaac taatttgcat caatggttcg
    10321 tgttccttta agcttccgct tgcttcacta attcctgcgt aattttcact gttggtcact
    10381 accacaatca ctgtatcatc gtagccagcg tttctaatgg cagcgaaatc ggcgttgatt
    10441 aataattgtc ctgctgacac ctgatctcct gcttttacaa atacttcaaa gggttcccct
    10501 tttaaattca cggtgtcgat ccccacatgg atcaataatt caataccttc tttagaacgt
    10561 agaccaatcg catgtttaga ttctaaaata gtaatgactt ctccctcaac aggtgaataa
    10621 atgctgtttt cttctggatg aatggctacg ccctttccca atagtccctt agaaaagaca
    10681 gcatcgttca cttcagaaat aggacacacg gttcctttta ccggattttc aacaatgact
    10741 gcttcggcta ttgccgtttg ctctgattca tcagaagtat cgtcaaatcc aattagataa
    10801 gcaatgataa aggttgcaac aaaggcgatt acacacgtaa taattgcgtg aacaatattc
    10861 attggatttt ctccaataaa ggctggtaag gctgctaagc caggggaaac aaaggcatat
    10921 ctgactaaac cgctcaagcc agcatacaaa ccagcaattg caccaccaat catggaagca
    10981 actaatggtt ttttcagctt caaggttact ccatagagtg aaggctcagt aattcccatt
    11041 aaagcagtga tcccagtgga cacagctagc tgcttcaact ctttgttttt agttttcgcg
    11101 gctacagcaa aggtagcacc tgattgagca atattagaag ccagcattcc tggcccattg
    11161 atcatttcag aacccgtact gctcaattgc atagtagcga ttggcgtcat tgcccaagct
    11221 gtacccgtaa ccaccaaaaa tggctgcaaa ccacccatca aaaatggaat taaccaagct
    11281 gctttgccgt taatccattc agcccccatt gctataagat cgttcagcaa cgttccaaac
    11341 ggtccaatga caaccaacgc taaaggcccc ataattaaaa ataccagcat cggcttagta
    11401 aagaatttaa taaacgaagg cgaaattcga tccgcaaagg gctctacata ggacatgatc
    11461 caaacagtaa agataatcgg taaaactgat cccgcataat ctgcagctaa taccggaatt
    11521 cctagaaaag aaacggcatc gccagtagaa aaaagctgtg aaatatttgg atgaagcaaa
    11581 atgccagcaa cagtaatcgc caaaattggg ttgacatcaa actttatggc tgccccatag
    11641 gctaatagta ctggcataaa ataaaaggct gcatcagcaa tagtattcaa aatgatgtaa
    11701 gtttgactac tgtctgatag tacaccagtc aaagtcaaga cggccaaaat ggcttttacc
    11761 atcccggcac ctgttatagc tggaatcatc ggtgtaaagg tggtagaaat gacactaatc
    11821 aatcttgaaa taatactttc ttgattgtct gaagaggggc cctttttaat tgaagttccc
    11881 acttcctttt gaatttcctt gaaggctgtt tggacttcat tgccaatcac cacttggtat
    11941 tgtccaccgc tatccaccac actaattaca ccaagtgttt tttctaaggc tttcgtatcg
    12001 gctttccttt tatctttgag ttcaaaacgc agtctggtgg cacaatgggt taagctgacc
    12061 acattttctt ttcccccaac ctgttgcaat acatttcttc ctacttctct gtaatccatt
    12121 attctctctc cttaaagcag taaaaaagac caagtcaaac cacgccccaa tcaacattga
    12181 agtgttagtt tgtcttggtc ttgcctgcat aatcagtaac atgccaataa cgttattttg
    12241 ctgtaatcca ataatagcac attatgatag cgcaatcaac agggaattta attttttatc
    12301 ttttcgtttg accgtatagg gtaatataac agttagatca catcaactag ggtatatctg
    12361 cgtatactta ctgctaaaat aagtatatct aatattaact ataccagtaa aaccattcct
    12421 acataaccag cgggaaattc ccaaaatcgt gaaagcatca aaaatgcata taacggatca
    12481 cacaacttca acaattctgc tcttgggaaa aattgatgaa tttggtgagt aaaggatgga
    12541 ataaacagca ttccaacaat ataggcaaca atagtgatac ttgttttttt ggtaaaaatc
    12601 agtaagcaat agccaatttc aatcattccg agatataaag gtatcaatag aatgatttgg
    12661 gctagtattc ttaaaaatta actagcaaat gtttaaactg atttatttct aatctacatg
    12721 tagtaagtat ttcaattcca ccaattatca ataaaaatac caaacatatc cataataatt
    12781 ttttcttttt catttctttt atattgttat aacagtgaat aacataaaaa ttcccgacaa
    12841 tcattgtaat atttttttgt cacctgtatt agtatttaat ttataggaaa ctataaaaac
    12901 tccctagtga atttacacct ttgggagttc ctgtttggac ggtatgcata atacatttaa
    12961 atcaatttaa cattaacacc tcagcatatg gtatctccac atatatacgg tctttctttg
    13021 gatagtactc aatatttatg ttgatagcac ttccattgtc taatatagct ttagcccggt
    13081 atgtgttatc tccattttca tgagtaatcg tcacatcatc acatgatcga tttaagttgt
    13141 actgttcttg taaaatctgt gttaccaatg gttttgatga cttctctaat ccagatgtac
    13201 ctgtcatttt tgaaaaaata atacctataa tagccaagac aacaatacct ataatgacaa
    13261 acttcttgtt catatttact ttattcaact ttttatctcc tatctccaaa ataatttgtt
    13321 cttatccctc ttatcagtac attgacatcc ctaaaagtac accaaagatt gctccagcta
    13381 cccctgctat catcaaaatc gtacccattt tctcatcttt ttctcgatat aagtagccca
    13441 taataacagc aacagcacca aatacaacag gaataaatgc taacgatact accatagaaa
    13501 tccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt tgttccttca
    13561 tttcattaat tgtctgctca gtacttttct ttactgtgtc tgttccggtt gtctcatctg
    13621 tttcttgtag tgctccacaa aactggcaat acttactttc atctgttatt tctttgccac
    13681 attttctaca atacatgtct tctcctccta aaatcattca attgcttatt agttaactgt
    13741 ttactttatt aaatacatca gaaaacagct cattcattgg tacacttaat atctcagaaa
    13801 gcatcttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa cttacacatt
    13861 gctgtgtaac attcattttt tttgctaact ccgtttgaga aagtcctttt tcttttctgt
    13921 atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca
    13981 atatgatgta tttcaactca taaaatataa aaaacttcaa ataccattac aaatacaaca
    14041 acttgttgta ctatactgag atgggggtgt attaatgaaa cagaaacaat atgcaaaggt
    14101 cattagagaa aagcgaaaag ctctcgggtg gacacaaaaa gaattagctg aaaaaatctt
    14161 ctctacacaa caggcagtag caagatggga aaattctgta acagagccta acctcgatag
    14221 cttaacagct ctttctagag ccctaggaac ccctgtaagc cattttctag acaatgttgt
    14281 cgtagattat gaggaagagt ttttggctct ctatcgttct cttagcactg aagatgcagt
    14341 tcgaacaatt gactatatga agctattaaa aaggcaagaa aatgaacgca atcagctatt
    14401 gaaagattga aaaataaata acccaccgaa ctttaacggt gggtgtattg agatgaaata
    14461 agattaagag ctatggataa cctgtctagt ttaatacaat tgggtctttt ctcttcctta
    14521 ctctccgtcc atcatctctt gttacacttg taaattgata cagaggaact atcccagctc
    14581 ctgataatat caattctttt attgtttcat ctatccctgc ccctttcgct aaatatatta
    14641 aaaaactact acttgctttc acattgtata gtttcttaat atccttagaa gctcgttcaa
    14701 gagtgaatcc ttgtttaaca aagtagcctc tgttttttat taaagcattt ttcacgctgt
    14761 tatccccaac attacgtagg cgttcacaaa cagtgttaat aagttctttc tctgtcatta
    14821 gatatgtccc ttgccacgat aagccctgta aaacttcatg tgcttcatct aaaatcaatt
    14881 gtttttcctt gctgagttct gatcgggaat tttgaggaaa tactctttta gcctcttttt
    14941 cgttaaaaca tatcttaacc cattcataag aaaaatattg atatgtaaaa tgaatttttt
    15001 ccagaagtgg aaggatatct tcattcaatt tttgaatcac agaagttgca ttaaaaacgt
    15061 ctaatcgata gacattgctt ctcttatgat taaagtttcc taacgctttc tcttccttta
    15121 acctatttag gtattcgagc atcttcaaag gaacatcttc atcatctaat ttttttatca
    15181 atcctataaa agtcataagt gatagtaact tattacattt atctatgtca taagttttac
    15241 catagtaaga actcattttt ttagctaatt gttcccccga caaaatacaa gtcacacttt
    15301 ccttactatc atcaaaaaaa ttaacatcag aaagcaagac atcatagaca cgtcgcattt
    15361 cctctagata attccttatt gtcctgaaaa gtttgtcttt cattgattca agagtgtcaa
    15421 aaagtacttt tgattcctct ttcaattttc gaaaatgtgt aggatacaag tctaggttct
    15481 ctaatagcca attaaatgtt tctacccgac tgtctttctt taaaattagc atcacaactc
    15541 caataaaatt ttcactgata tcacaattac tggaatgaca ataaaaaatg gaatgcccag
    15601 ttttactggt aaaaatacta gcagagggat tatgatcgtt gtgaaacaga catcttaagt
    15661 ttttacttga cttaagtaat ttattcatat caatggttaa caatctttca taaatctcat
    15721 taaaagacat gtcggaggaa tcaaacaggc agtctttaaa ccactgtttc atttcttctt
    15781 tgtcgtcatt ttttatcaac ttaacaaaat cggtttcatt gactagttca tactctctcc
    15841 ctgaatcact ttgacaattt ttagaacatg tcatggtaaa atcaatcgga agctcagtaa
    15901 tatctaacat gttgtcttca ttaattatta tcactgcatc tgacttatat cctccaaaaa
    15961 acattctatt cgaagcattt acattgtcat cacacccggg gatgttcttt ttcaagtatt
    16021 cgtaaacagc aaccatttta ccatagtcag taattgcttc ttttaataga aagacaatct
    16081 tgtatttttc tagattttca gagtaccgaa tacttttttg tatcgcaaac gcagaattat
    16141 gaactaaatt gatagataaa accttttcta tgcttaagta tctactatca tctttactca
    16201 aatctactac cttccccttc tcatctttaa cagtgttatc gacatccaga ataattactc
    16261 tggtttccac aaaactctcc ttgtcattgc ctttattctt tgtcttgctt ggaataaatg
    16321 attgtgatga atttagtatc tctacaaact catctcttgt aacttctcta tagttatcta
    16381 aacacctatc tcttacatca gttttttctt tttttgtaac tggcttctct aataattttt
    16441 cttgatctac ataaattgga atattaatca ttgatgtttc catttgtgtt tcctccttat
    16501 aaaattctgt taaagactaa aacatacaga taataaaaac aaactcttta ttactatatg
    16561 ttttagtctt atttgtttac tcaaccttta taccgttcca tgaaactatc aatatcagat
    16621 tttttaaatc taatgaccgt atcaattttt ataacaggta acccgtattg agaaacatat
    16681 tttctaatcg ttcctactga tacacctaga tattcacttg cctgctgata gtccatatac
    16741 tctagagtga ctccagaaat aatgtcttct agcataggct ttagcactgt aattatctct
    16801 ttcacttgtt gttcaacttg tatagaaaca atgctatcaa tgatttgtag agacatattt
    16861 ccccctcctt tcgtgattgc ttgaacaaat tttctaagaa ttatcttcgt attttttgct
    16921 caaaataaaa gtattttgtt attaaccgat taatcaaaca aaaaatgatt accagttcat
    16981 cgacatacac ataatacctc gataaaaaag gggtgtcaat agttttgtaa aatttattta
    17041 caacacccct tttttgaggt atactaattt taggaggtgt ggtcaatatg ataaaacgtc
    17101 caccaataaa ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa gctataagaa
    17161 agagtaaaaa attaacacaa ccagcatttg gtcttatgat taacaacggt caattaattg
    17221 acaaaaagac tatctacgaa tgggagaagg gaacctattt accaatacca gagcgattat
    17281 cccgaattgc tgatttggga aatatgagta tagaggagtt agtttgtggc aatgtagaag
    17341 aatatatttt gggaattatt ctttatagag atagcattgt actagatggt ataacatttc
    17401 cagataaaaa cctgttccag catcttcgtc aacagttccc accagtacac tctaatttag
    17461 atacatggct ggatagatat tccaaattag aaccagagat gcaagaattt attgctaaca
    17521 agacttgtaa taaagttaaa aatgaaaaaa ttagtctttt caatatctta aaaatagaag
    17581 aattattcat caatgctatt gtagaagaat ttgacaataa cattcttttc ctcactagca
    17641 gtatagaaga actactagaa agaatggtag atgaatggct acctattcaa ttgaaagaca
    17701 tgtcttatcc cgaagaagct gtgagagaaa ttactgataa tataaacaag ctagaacaaa
    17761 cgatttcatc aattgggaaa aaatacacta agaaaaaaat gaagggaggt gataccatct
    17821 agtggtaagt actccttgta aactaagagg agatgatttc atggcaaaaa tatcaaagta
    17881 caaaaagaaa aacggtcaag tggcgtggat gtataaagga cacattgcca ccgatccacg
    17941 aactggtgaa aaaataaata ctacgagaag aggttttagt agcaaacaag aagctcaaaa
    18001 agattatgac gagtacagac atcaaatatt atacggtgtt aaaaagaaag cacctgatat
    18061 gacatttgaa gacctgtata atgaatggat aactcatcag cgtaccagtg ttaaagcttc
    18121 aacaatcgca atctcagtaa gatacgcaaa taatcaaata cttccagcat ttggtaagct
    18181 taaactatcg aatatctctg tcccatattg tcaaaaagtg gtagatgaat ggcacagtaa
    18241 atatgaaagt tacgattata tgagaaaaca gactgcacaa attttacgct acggggttgc
    18301 aatgcaatat attgacaata atcctatgga aaaaacactt cttccacgaa aaaaagagta
    18361 tgagaaaaat cgtaaatttt actcaaaaga ggagcttaat aatttactgg atgcttttaa
    18421 agattttggt aacatgaaac aatacgcctt cttcagatta ttggcataca caggtatgag
    18481 aaagagtgaa gtactcgctt tacagtggaa agacatagac acgtttaata aagaactaca
    18541 tgtcaataaa actcttgctg tagatgaatt tggtaaagtt ataattcaat ctccgaaaac
    18601 aagagcttct agacgagtta tctctttaga tactgagact ctttctatcc tcaacaactg
    18661 gaaattacag caaaaagaag agtatttaaa acttggatac aatactagct ctaaagagca
    18721 acatgttttt acaacagtta aaaacactct atacattcca aacactgtta atgattggtt
    18781 aagatatatt ttgaaaaaat ataacttgcc tcgtattaca cctcatgggt ttagacatac
    18841 acatgctagt ttattattag aagcaggaga atctgtcaag gtagttcaac aacgattagg
    18901 gcatgaaaat agtaaagtga cattagacat ctatgcacat attacaaata atgcacctaa
    18961 gaaaacaggt caagactttg ctgatatgat ggctcatcag taaaagagct ggtcaaaatc
    19021 tggtcatttc tagtatacag gcaaaacaaa aaagctagaa cccttatata tcaagggttc
    19081 tagctcctat aacaggttta cccaacactg ccttccatct cataactgat caatcgattg
    19141 agttcgactg catattccat tggaagttct ttggtgaatg gttcaacaaa gcccatgacg
    19201 atcatttcag ttgcttcaga ttcggtcagg cctcggctca tcagatagta gagttgttct
    19261 tctgagattt ttgagacttt ggcttcgtgt tctaaagaga cttggctgtt gtggatctca
    19321 ttgaatggga tcgtatcgga tttcgaccat tcgtccatga tgatcgtgtc acattcgata
    19381 tgagagatcg aacccgcact gtttttggca aaggttactt gtcctcgata gtttacttct
    19441 ccgccgtctt ttgaaattga tttagagacg atagagctag atgtatttgg tgcattgtgg
    19501 atcatttttg cacccgtatc ttggatttgg tttgctcctg caaaggcgat ggagagcatg
    19561 gtgcctcgtg caccttttcc atctaagtaa acacttgggt atttcatcgt tgtttttgca
    19621 cctaggtttc cgtcgatcca ttcaacggtt gcgccttcgt atgcttttgc tcgtttggtt
    19681 actaagttgt acacattgtc tgaccagttt tggatcgttg tgtaacggca atacgcatct
    19741 tttcttgtaa agatttctac gatggcggca tgcaggctgt tacttgaata agtaggtgcc
    19801 gtacaacctt cgacatagtg gatgcttgct ccttcgtcaa caatgatcag cgtacgttcg
    19861 aattgccccg tgttttccgc attgatccgg aagtacgttt gtaatggaac atcgactttg
    19921 acgccttttg gcacgtaaat aaaggtccca cctgaccata cggctgaatt cagcgctgct
    19981 aatttgttgt cggttggcgg tacaagtttt gaaaagtact ctttgaataa ttcagggtat
    20041 tctttcaaag cagagtcagt gtcagtaaag acgattccta acttttcgaa ttcatctttc
    20101 atattatggt acacgacttc tgattcatat tgtgcggaag caccggctag ataagctcgt
    20161 tcagcttctg gtataccaat cttttcaaat gtatctttga ttttttctgg tacgtcttcc
    20221 cagtcacgag cgggtttgtc acttggtttt tggtaatatt tgatggcatc gaaatcgatg
    20281 tctgaaaggt cggggcccca tgtttgcatt ggcattttgt taaatgtttc tagagatttc
    20341 agacggaatt caagcatcca ttctggttcg cctttgattc ttgacatctc acggatcact
    20401 tcttctgtca agccttctcc tgtactgaaa accggttcga catcatcgtg aaacccgaat
    20461 ttatattctt ctaattctgg tacgcccatg ttctcactcc tcttccttgt ggtaatgatg
    20521 gaaagctgcg gtaccatcgt tttccattgc ttttcctagt gctttccagg caagtgtcgc
    20581 acatttgatc cgagcgggaa atttagctac accaccaagc atagcagcat cgcctaattg
    20641 gtcgggatct ttgacttctt ttccttggac aagctgtaga aagtctacag ttaaatgttc
    20701 tgcttcttct attgattttc cgatgacggc gtctgtcatc atcgaggcac ttgccgtact
    20761 gatggagcag ccactgccgt caaaggcgat atttttgatg attccgtctt ttacatcgac
    20821 ttgcaattcg atcacatctc cacatgtggg gttgttcaat tcgatcttgc ttgtcgagtc
    20881 agtcaagctt ccacgatgat gcggatggga agaatgatcc aagatcactt gacggtataa
    20941 attgtctaat ttagatagtc ccatgttgga aaaactcctt tgttgccatg attgcttcaa
    21001 ctaaacggtc ggcgtcttgc gttgtgttgt agaaataaaa gctcgcgcga gcagtcgctg
    21061 atacttcaag gtacttcaat agcggttgcg cacaatgatg acctgctctg acggctaccc
    21121 cttccatgtc taaagctgtc gcgacatcat ggggatgtaa cccatccaaa ttgaatgcaa
    21181 cgactcctgt ccgaaaattc gggtcttgtg gaccataaac agtcaatcca tcaattgctt
    21241 gtaattttgg caacacataa gcggctagtt cttcttcata tcgatggata ttgtccatac
    21301 cgagtttttg caaataatcg attgcatggc ctaatgcgat agcgccggcg atatttggtg
    21361 tccctgcttc gaatttccaa ggtagttctt tccatgtact gtcgtataat tcgacaaaat
    21421 cgatcatttc gccaccaaat tcaacaggtt ccatttcttc taaccatttt cgtttaccat
    21481 ataaaacacc aatacctgtt ggtccgcaca ttttgtgtcc gctgaaagca aagaaatcac
    21541 agtctagtgc ttggacatca atggccatgt gcggggcaga ttgagcacca tctactacga
    21601 tgaccccgcc gttttcatgg acaagttcag ctaattcttg aacaggattg aagacaccta
    21661 agacattgga tacatgagca atggagacga ttttggtttt gtccgtaatc tttttcgctg
    21721 catcctccat atccaggaag ccttcttctg ttagttcgat atatttcaag acagcacctg
    21781 ttcgttttgc caactgctgc cacgggataa cattagaatg atgttccatg taagagagca
    21841 ctatctcgtc tcctgctttg atatagcggt cgccgaaggt tctggctacc caattcaagc
    21901 ttgtcgtcgt accacgagta aacagcactt ctgctgtttc ttttgcatgg atgaacgccc
    21961 gtactttttc tcttgcttgt tcataatcat gggtcgcgcg ttctgctaac gtatggacac
    22021 cgcgatgaac attggcgtta tcgtgttcgt aatagtacgc taatgcctgg agtacttgtt
    22081 ttggcttttg ggtcgtcgct gcattatcca gataaactaa aggttcatca ttgacgtctt
    22141 gaaataagat aggaaaatca ttgcgtagct ttttagcatc gatcatccat tcaacttccc
    22201 ttcgattact tcgactaatt ctttccgaac ttgttctaca gggattgctg ttaagacaga
    22261 accgaggaat cctcgaatca ctaagcgctc tgcttcttct ttatgcaatc ctctactcat
    22321 cagatagtac atttcttctg gatcaactcg tcctacactg gcagcatgtc cagcagttac
    22381 ttcgttttca tcgatcaaaa gaataggatt cgcatcacca cgtgctttat cagaaagcat
    22441 caagacacga ctttcttgtt gggcatctgc accttttgcc cctttcaaga tgtggccgat
    22501 accattaaag gtcaatgttc ctttttctcg gatcactccg tgttgcaaaa tatgcccgat
    22561 cgtatgaggg gctttattgg ttactcgtgt atcgattcct tggatttgtc tgcccgagct
    22621 gattgccacg attttgactt ctgcgtgtgc accttctcct gctaaatctg agtcaaagtc
    22681 agcgatcaca tgaccgtcat tcatgacacc aattgcccag tcgacagaag catctcgtaa
    22741 aatgtgccca cggcgattca tataagaagt aatattttct cctaattgat ctacggctga
    22801 atatttgatt tttgaaccag ctttagcgat gacttccacg atgatattac ctgatgattt
    22861 tgctacttgt tctttggttg tctggaaacg ttccagataa gaaaattcac tatgttcatc
    22921 tgccacgatc aacacatgct tgaagaagtg ctcatcggat gcgccatctt gaatgaataa
    22981 ggactcgagt ggttcatcga tcacgacatt tttaggcaca tacaggaaaa taccgctgtt
    23041 cataaaagct acatggagtg cagtcaattg gtcttcgttc atgtttactg cttttttcat
    23101 ataatattct tggactagtt ctggatattc gatcattgca gtgaacaagt cggtaaaaat
    23161 gactcctttt tctgctaatt ccacaggcag ttgttcaaaa atcgtccatg aaccttgttg
    23221 gacaagcaca ggattgtctt tcattgcatc aaatgccgga atcgtgcctg tttcactgat
    23281 ggtttgtgtt tctttcacat caaataacga ccagcgatgg aatttcaccc gatcgatatg
    23341 cggcaatggc agttcatctg ctttcgctag tgcgtcttga cggaaggttc tcatccaatc
    23401 aggttcgcct ttacttaaag aaaaatcatt taccgcgtca agataatcga gccagttttt
    23461 ttctttcatt cttgctcccc cttacgcttc ttcttcttta tagtcgattc ctaattcttt
    23521 actgatacca gcgtagcctt ctgcttccag acgtttagct aggtcagcat tccctgtcat
    23581 gactactcgg ccttccatca tgatgtgaac gacatcaggt gtgatatagt tcaataatcg
    23641 ttgatagtgg gtgatgatca atgcaccgaa attgtctccg cgcatttcat ttactccttt
    23701 agcaacgact ttcaaagcat cgatatccag acctgaatcg atttcgtcta aaattgcaaa
    23761 tgtaggttct aacatcaaca attgcaggat ctcattccgt ttcttttctc cgccagaaaa
    23821 accttcgttc agataacgtt cagccatttc ttctggcata tttagtaaag ccattttttc
    23881 atctaacttt ttcaaaaatg ccataacaga aattttgtcg ttttcatcac gtttcgcatt
    23941 gatcgctgca cgcatgaatt ccgcattcgt gatacctggt atctcacttg gatattgcat
    24001 agctaaaaat aagccaagtc gcgcacgttc atctacttcc aagtctaaaa catttttccc
    24061 gtcaaacaaa atttcgcctt ctgttacttc atagtttgga ttgcccatga tagcggcaga
    24121 taaggtagat tttcctgtgc cgttaggtcc catgatggca tggatttctc cggttttcat
    24181 tgttaggttt acgcctttaa ggatttcttt ttcctcgatc gacacatgta gattttttat
    24241 ttctaagaca gacatgcatt atacccccaa attctctttc gttctcactc attttagact
    24301 aattgagaat gaagcgcaat gaccttcctt actaaaatca aatatctttt atttattatt
    24361 aaacaatttt gatttctaaa ttagaatagg tataaaaaag atgagaaata agccattaca
    24421 gacttatttc tcatcttttt tgttccatac cttattttgc atcagccttt actggtttaa
    24481 cagagccgcc ccatttgtct gtgatccatt cttggacttc tttgcggtga agcacttcaa
    24541 tcagtttttt gattttgtca ctgtcttcat cacctttacg agtcgcaata atattcacgt
    24601 aaggagagtt gtccttttca agcaatacgg catcttctaa tggattcaac cctgcgccgt
    24661 aagcgaagtt tgcgttgatt gcaactaggt caccttcatc attttggtac gtagaagcta
    24721 aaagtgatgg gtcgatcgta tggttgaact tcaagttctt agggttttct tcaatatcat
    24781 cgaatgttgc tgtttccaga tctactccat ctttgacttt gactaagcca gcatcttgta
    24841 gaattgtgat gatgcgtccc cagtctgatt cagaatttga agtaatgatt gttgcaccat
    24901 cttttaattc ggaaacatct ttgacttttt tagagtacaa gcccattggt tcgatgtgga
    24961 cagcaccggc attgacaaag tcatatcctt tttctttgat ttctttgttc aaataaggaa
    25021 tatgctggaa gtaattggca tcaatgtcgc cttcttccaa agattggttc ggtaatacat
    25081 agtcatcgaa tttgacgatt tcaagatcga ttccttcatc tttcaataat ggtttcacat
    25141 gttccaaaat ctctgcgtgc ggtgtcggtg aagcgccgac tttcaatgtc gtgtcgtctg
    25201 ctttatttgc tgaatctttg ctgtcgctgt tgttaccgca tgctgctaat cctacagtca
    25261 gcaaaagcgc tgctgcaaat ccaaaaattt tctttttcat atcctacatc cccctatatc
    25321 ctattatctt ttgtctgttt tcttagttaa gaaatcaccg attccttgaa cgataaatac
    25381 gattagtaaa ataattaaag tcgcaacgaa tgtcactgtt agattaccac gctggtagcc
    25441 ttcctgccaa gcaagacctc caagtccacc tgcgccgatc gcgccggcca tcgcagtaaa
    25501 tccgatcatc gagatcgttg taactgttaa tccagaaatc aatgcgggaa tactttcagg
    25561 tatcaagacc ttccagatga tttcccagta acttgctccc attgcatctg ctgcttccag
    25621 tacaccaggg ctgacttcac gaaaagcaat ctctactaga cgggcataaa atggtgctgc
    25681 ggataaaaca agagctggaa tggctgcttg cgcacctagc atagtgccta cgattgcttt
    25741 agtaaatgga atgatcaaaa ccatcaggat cataaatggc gttgaccgga aaatattact
    25801 gatgatcgat acgattccat aaatgattcg agcatacggt ttgtttttgc gtccaataga
    25861 gtaaaggatc aatcccaaaa ctaatccgat gatcgtcact aagaccattg agattaatgt
    25921 catatatagt gtatcaagga tagcctgatt catcgtttcc gggttgattt gtttaaaatc
    25981 tagatatgtt tctgaaaagc ttttatccac gatggatcac ctcaattcct actccttgtt
    26041 ctttgatgaa cacttctgct tgatctaatt ctttggtttc accagtcatc aagacagtca
    26101 atgaaccaaa ggaaccttct ttcacttgtt tgatctttcc atacacaacg ttgacatcga
    26161 tgttatagcg tcgaatcgct tgggaaatga ctggctcgtt ggcatttctt tcgctaaagg
    26221 tcaaagttgc caaacggccg cttggatttt ctttgatgag ttcagctaat agttctgtcg
    26281 aatcagcttc tggttcgatg tcttgttgta caaagcgttt ggttacttct tttttcggtt
    26341 gtctgaagac agtcaatacg tctccttctt ctacgacttt ccccaactcc atcacagcga
    26401 ctttgttgca gattttgcgg atcacattca tttcatgtgt gatcaagacg atggtcaaat
    26461 tcaatttctt attgatcgct aacaataagt ctaatacttc ttctgttgtt tgcgggtcca
    26521 atgcgctagt agcctcatca caaagcagga tctcaggatc atttgccaat gctcgtgcga
    26581 taccgacgcg ttgtttctgt ccgccggaaa gttgtgaagg atatgcttgt ccgcgtcctt
    26641 ctaacccgac taaacgcaac agttcttctg cccgttcttt acgtttgctc tttggtacgc
    26701 cagctaattc cagcggcagc tggatatttt caagtacagt tcttgaccat aacaggttga
    26761 aatgctggaa gatcataccg attttctttc ggaatgtacg taattctttg ttttttaatt
    26821 ttgtaatatc ttgatcgttt acgatcactt cgccgctcgt gggaagctca agtccattca
    26881 ggagacggac taatgtactt tttcctgcgc cagaatagcc gacgattcca taaatatccc
    26941 ctttttcaac gtttagtgaa acgtcattga cggcttggat gtttccgttt ttcccattga
    27001 atgtcttttt gacattcgtt agttggatta atgacatgcc attcacatct ttctcttata
    27061 atttttcaaa caaaaaagcc ttcgccctct tgccttaagc aaaaggacga aagcttgaac
    27121 ttccgtgtta ccaccttgat ttaccgttac ttcgcaataa cagcctcttc cagtacttgg
    27181 acttagcttc tttccgtata ctgtcgcgct gtaacgggca ctcccgtagt aagtttgctt
    27241 acgctcgctt actctgctct aagaccatct tccatctcat tttcgttacc cattttcagc
    27301 aaacagggct ctcttcaaac gtccgtgaca tgtactcttc ttttcaaagc tttctcgttt
    27361 catataggat tgcttatgat tatagcaact ggaagaatca gtgtcaactt ttttcaagtg
    27421 attttctgat tttataattc gtcaaattct tttggatcaa catctttgaa acttaagatc
    27481 cacgcattca tttcatctga atcattcaag atattgatat cttcaccgat tttatcatta
    27541 acagaagaaa ctgtacccgt tagcgggctg gcaaattcat tgaccgcttt ttccgcttca
    27601 acttcgatca gaggttcatc tttttttagc gattgtcctg cttttggcag attggcaaac
    27661 gtgatgtctc caagttcttc ttgcgcttca tttgtcaaac caacacagta ttcttttcca
    27721 ttaaataaga tccaaaggtt gtcctttttc ttcaaatgct tgcctgccat tttatatcag
    27781 ttctccttca taaatttttt cattgaatcc aattgcaaca agctggtcct ctcgtaagat
    27841 cagtggtcgc ttgatcagca taccatctgt cgataagact tggctcgctt ttgaagccgt
    27901 aaactcgcct acttgatctt tcagtccaag ttcccgatat ttcatcccgc ttgtgttgaa
    27961 caggcgacgg attggtactt gactcgtttc tagccaagtc tcaatcgttg aggccttcgg
    28021 tacttctttg atcatatcaa cggtttgata actaacgcca tgctggtcta accacgcttt
    28081 ggcttttttg catgttgaac atttcgggta ccagtaaaag gtgtacaatt ttcttacctc
    28141 cttgaagatc ttgcaggtgc ttcattttgg tatctcatcg ataatatcaa cccgataccg
    28201 atcatgttcc ctaaaatcga ggatcctccc tgactaatga aaggcaatgg gatccctgtt
    28261 aaaggaagta atccgatatt tgctccaata ttctcaaaga catggaacag cagcatcatg
    28321 atcaaaccgc ttgcgatata ggcgtaaaat tcattattcg tatcgaaaca cacccgaatc
    28381 atacgataga ttaaaataaa atacaagaaa atgacgaatg caccaccgat aaagccgaag
    28441 ttttcaccga taacggtaaa aatcatatcg gactcacgaa caggcacata gatattgctt
    28501 acgttgaatc ctttaccaaa caagccaccg gtaccaattg ccaaaagtcc ttgggccggc
    28561 tgataactca ttcctgacgt atcatggaaa gggtccagcc atgaatcaat acgtgcaaac
    28621 tgataagatt tgaatccaac tttataaagc aagtctcttc cgccttcagt cgtaaccaag
    28681 aagatcgttc ctgctccaac gactactgcc aagattacta caggtgcgat gatgcgccaa
    28741 gagattccag acatcaggaa aatcccgcca aagatcgcaa gaaagacaag catcgttcca
    28801 aagtcatctt gggccatgat caatgctaaa accggtattg tcacgatcaa gagcttccca
    28861 atcagccaaa agtccgattt catcgtccgt tcttttactt gcgtattatg tttagttacg
    28921 atcagtgcca tcatcaagat gtacgcaatc ttcatcaact ctgctggctg gaaagtaaac
    28981 gcaccgaatc gaaaccagtt tttagaccct gttgaagcag ctaaattctg atcatagaat
    29041 ttgagcaaga gtcccattac cactaacccc aaaccataaa ggtaaggagt cagtttccag
    29101 atccatttgg aattcaaatg catgatgaca acgattgctg ccgctccaac taaataccag
    29161 acaacttgca tccctacgcc tttcaagact gctggattgt tcggatcatg gctaagtgct
    29221 acatataatg agagcagacc aatcaaacat aacagaaaga ctggtaaaat cacgccataa
    29281 tcaatacgat tatcgttcga agcttttaat tttttttcca tgtttcatcc cttttcttaa
    29341 aaaccatact gctgattatt atagcggtcc agttagataa tggaaagtct ttccatagac
    29401 atttgggaaa aattaacttt ccgtttgtgc agttgcttga gcaaatgcag tacaaaagcc
    29461 tactgcttat ctttagtgta tcatagaact ttttttgata catgcaatta tctaccagct
    29521 gagattctcc tcgctgtgag aagtttctgt gctttcattg tatcgaagga tctcttcggc
    29581 aatctctagc agctgatcag aaaccagatt ggttcgttgg atgaaataaa aatatcgatg
    29641 gaaattcaac ggagtcattg gcacattttc tggaacagag cgcttagaca aaatcgagcg
    29701 accgattcct tccttcagca acgcaacaat tacatcgtta cttttcacga tcatcttctt
    29761 atcttggata ttcatctctt caaaataacg ctgcgtgtaa tgaaaaacac cagagtttgc
    29821 ttctcttaca agccacaatt tgctttttgg gtccccagcg atgactagct ggtcttccat
    29881 caaagagtat ctttgtacgc cggaagtctc taatggcttt tcgataaaac caaaatcgga
    29941 ttcatgtttc gctacttgtt ctaatgattc atgagaattc gtgatttcga tctcaaaatg
    30001 cactttagga aaccttttga tcagatgttt catcaactga ggcaaaagat agacagcaaa
    30061 ggtgtgggaa gcaacgatcc gacatacttc ttcttcattt tgttcattga gcaatttttg
    30121 ctgagtttct tcccattctt ctaacaaaac cagtgtccgt tcatataata agtcggcttg
    30181 cttagttgga agcatctctt ttcttccgtt acgttgaaaa agatcgatat tcaattcccg
    30241 ctccaactgt ttgatttgat tcgagactgc tggttgagat aaaaacagta cttctgctgc
    30301 tctggaaaaa tttcttgttt catatactgc tcgaaaagtc gttaataact taaacatggc
    30361 accacttcca taacttttta tgataattac tataaaaact atttgattta aatatacaaa
    30421 aaaccatcta taatagaaat gtacaacaaa acagttgctg atccaactag taacttagat
    30481 gaagacaatc agctgtctgc ttgctcgtaa tcatacattc ctattcattt tacaatgtat
    30541 tcgttcatct tggtactaaa atctattaaa taaaaaggag aatattatat atgaagaact
    30601 ttttgaaaat tattccgatc ccgatctgcg ggttgatcct tgggttagct tcccttggaa
    30661 atctgtttaa agactatcac catgttgctt taggcaatct aactggcggt atctctatga
    30721 ttcttatgat attcattttt gttaaattgt ttatcttatt cgaacacacg aaacaaacgt
    30781 tgcatgatcc aatcatcgcc tccgtttcac caacttttac gatgtcactc atggttattt
    30841 gtacttattt tgtttcatat gaaagtattg caccaattgt caaattcatt tggctgtgcg
    30901 ctgttatctt ccaagtgatc cttgtctttt atttcaatta tcaccatgtt atcaaagctg
    30961 atctttcaat tgaagcaatt tatccaagct ggtttatcat ttacgttgga tttggggtca
    31021 tcactgtaac tgccgggaac ttcttcccac ttattggaaa aattttcttt tggatttcat
    31081 tgatctgcta tgcgttctta ttgccgatca tcatcaaacg tgtctttttc gtaagaaata
    31141 tggctcatcc gacacgtccg ctgattacga tcgttgcagc acctggttct ctttgcctaa
    31201 caggttatct taaaaacttt gctgaaccta atttttatct tgtcgtagca ttgttcgctt
    31261 tatctcaagt attgtatttc gttgtgctgt ctatgcttcc acgtttgttc aagcttcgtt
    31321 tttatccaag ctatgcagcc tttacattcc cgcttgttat ctctgcaaca gctatattta
    31381 ctactgttca ttacttcaac agtttaggta tctatagtag cgtattggat ttcttaaaag
    31441 ttgcggaatg tctgtttgca agtgtagttg tcttctacgt tcttgctcat tacgtacgtt
    31501 tcttaattaa agaacatagc aaacatggaa aatttgtttc taaaaaaatc gcttaataat
    31561 cgattcaata ggttgtgaaa aagctgttct gcatcaagta ataagaacgg ccaaacaaaa
    31621 acagctgttc atgtttttgt actggttggg cttattaccg cagatgcaag cttttgaaca
    31681 ccgtttacca agaggtcgta aaagaagtgg tcagcttcaa gaaataagaa ggaatttacg
    31741 aaaattgctt ctcaaatttt tgtgaaattt cggcttattt ccgtagaagc tacttctgga
    31801 acaccgttta ttcgaaacaa gaggtcgtac aagacttttg tcacgacctc ttgttctttt
    31861 gtcaaaatac tggtttagtg ctactatgtt cgagtatgct tttagtattt attgacgaaa
    31921 ggaaccgatc aaatgaaatc attgtcacat ccaataaaat ctgtaaagga ttcagcggtc
    31981 cgcgaaatct tacccggttt actgctttct tttgccatcg ctgccttcag caggcttctt
    32041 gctatttatc tgccacagtt aggtggagcg actattgcta ttttattggg tattttatta
    32101 ggaaatactt tttttaaaca gcctgttcta gcaatcggca caaaatttgc tgaaagcaaa
    32161 ctattagaat tctctgtttt tttactaggt gcaactgtca cagctcaaac gattgctcaa
    32221 attggtgtaa aaggaattgt ttttgtgtta ctgcaaatgt cagtcactat catcggtgct
    32281 tacttgattg gtaaaaaact gcaattcagc gatacttctt ctttgatgat ggctggagga
    32341 aacgctgttt gtggttcttc tgctattgct tcaatcgccc cttccatcca tgcgaaagag
    32401 gaagaaaaag gacagatcat cacactagtc aatctactgg gtactgtgat gatgttgact
    32461 ctcccttttc ttggattaca gttgtttggt ggaaatctca ttgaaaaaag tgcacttttg
    32521 ggtggtacac tccaatctgt tggtcaagta gtcgctgggg caagcttact tgacaattct
    32581 gttgtcgaat tcgccatgct atttaagatt ctacgtatta tgatgctcgt tgttgttgtc
    32641 tatctgtttg aaaaattcat gcacaagaaa caaccagctc tgtttgaaac gccaactgat
    32701 acgcagaaaa aacgaaaatt accgcttcct tggtatgtat tagggtttgt gatcttttgt
    32761 ttggtcaaca gcttgttttc tttacctgta gttattgacc aatcggcaca ttttatcagc
    32821 agctggtttg agatcactgc tttagcggca attggtctgc gtttagactt tgcaaaattc
    32881 atgaaagaag gtacacgctt cttatcttat ggattagctg tcgggcttct ccaaacagtg
    32941 atggctttac tatttatttg gctgatcaaa gtttagaatg aacatgaaaa aacagttgtg
    33001 attctcgcat ggaacaatgt ccgaatcaca actgttttta attttgcaga tatccttcta
    33061 acaacaacaa ttggcgtttg atttcaaccc catctattgc gtttcccata tatcctgtca
    33121 gttttccatt ttgcccgatc actcgatgac aaggaataac gattggcaat ggattggttc
    33181 gatttgcctg cccaatcgct cgcacagctt tcggactatc aacagcaacg gctacttctt
    33241 tatacgttcg gatttctcca taaggaatgg tcgacaaagc tttccagaca ttttgctgaa
    33301 aaggcgtacc tctctttata gataaaggaa catcaaattc ttttcttttt cccgcaaaat
    33361 attcttcaaa ctgttcttgt gtgcgcgtta aaattcgctg attcgctgat tcgctttctg
    33421 aaattggctg aaaagataca gcagtcaatc cggattcatc tgcatctaac cataaaaagc
    33481 cgataggtgt gtgtattttc attttgtttg ctcctagtca atagactgga acttcctcct
    33541 ttttctctat ccaaaactac ttgccaacta ataaaaattg ttcttaaaag tttgataaac
    33601 cgaggaaaca tggtaaacta aatacgttag tttacaaaaa gaatacctgt tcttttacgt
    33661 aaaatcaaat tttaaaggtg ttatttaagg tttattaaat tttgaatgta aaccatatat
    33721 aaacatacga ttgaaaggat agaaacgatg aaagtatcaa ttgttgtccc atgttttaat
    33781 gaagaaaaaa ctgttcctct tttttttgat gaggttgaaa aattcaaagg tgcccatgag
    33841 ttcgaatatt tgtttattga cgacggatca aaagacggta cgttagcagc aatcaagaag
    33901 ctagcctcag cttatcctta tgtccgcttt gtctcatttt ccagaaattt tgggaaagaa
    33961 gcggctctgt acgctggatt gcaagcttct acaggagatt tagtgaccgt gatggatgtt
    34021 gatctgcaag atccgcccga actgcttcct gagatgatca accggatcac tacttctgat
    34081 atcgattgtg tcggcacccg gcgttcgact cgtgaaggcg agcctgcgat acgcagtttc
    34141 tttgcgaaaa aattctacca gttgatcaat aaaatcagcg atacagaaat ggtcgatggc
    34201 gctcgtgatt tccgcttgat gactcgtcaa atggtggatg ccgtcctaga attaacagaa
    34261 tacaaccgat tttcaaaagg attatttagc tgggtcggat ttaaaacaga atatatttct
    34321 tatgaaaacc gcgaacgtgt ggctggcgaa acatcttggt ccttctggaa attattcaac
    34381 tactcgattg atggtatcgt gaacttctcg gatgcaccgt taacgattgc ttcatttatc
    34441 ggcgctttgt cttgtgtcgg ttctggtatt gcaattttgt tcattatttt gcgggcattg
    34501 ttgtttggtg atcctacatc aggatggcct tccatggtct cgatcttcct gttcattggt
    34561 ggaatccaat tgttatgtct tggaatcatc gggaaatata ttgggaaaat cttcttagaa
    34621 acgaaacagc gtcctgttta tatcataaga gaaacagaaa aagtgaagcc aaatgaattg
    34681 aagcctcacc aataatatac gtcaaatgaa gtaaacattt ttttaaatga aaaaggactg
    34741 tgacaaaagt cttgtcacag tccttttttc cgaataaatg gtgttccaga agtagcttct
    34801 tcggaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
    34861 tttcttgaag ctgaccactt ctatcacaac ctctttcata tcttattact tcttcattgc
    34921 tgcttttagt gctgctgcta atgggctttc ttctggttct tctgcttgtg catatttttt
    34981 cagcaagcgt cgttcttcat gtttggtcat tttcttcttc tgctcttttt tatcaagcaa
    35041 tttttctgtc gttccatcgt atttacaacg gaaataagca ccgtttttac cttcgatgat
    35101 ttccattttc cggtgacatt gcgggcatcg atgattagag acttttggat cttttcttct
    35161 gcggtagttg caatctgaat tggtgcagac atagattttg ccgtcacgtg tgtttttctc
    35221 gcgcagattg gccccacatt ctgggcattt cttttgggta atcgagaaat cttgatattc
    35281 ttgtttgctt gatttgatct cattcaccaa gcgttttgtt tctttttcga ttccattcaa
    35341 gaaggttcct gcttgtttct ttcctgcagc aatagcttca aggtcttttt cccagctttc
    35401 tgtcaaatct ggtgttacta aagagggatt taccaatact aataattgct tacctttagg
    35461 agatacttgc agtccttgag gggtacgttc catcagttca gatttgatta gtttctcgat
    35521 aatctctgca cgcgtagctg gggttcccag actgtatttt tccatttgac caagcaagct
    35581 tgcttctgac aatggtttag gcggtgcaga tagttctttt ttgatggcga aatcgggatt
    35641 gattctcatt cctttttccc atttgactgt ttccgtctca gtttcagttt cgcctttcca
    35701 tcctgcttgg acgacacgat tttgccgaaa aatgaatcgt tctttaccaa attcaaccgt
    35761 aacttttgtt tggctgactt tatggggttg agcaaacagt cctaaaaaac gagagacaat
    35821 catttgatag atttttcgct cttcattact taatttttca taacgtgctc tgttttcagt
    35881 tggcaaaagt gcatggtgat cggtcacttt actgtcttgg aatacttttg tctgttggac
    35941 gatggctccg tttttcaaat actgcttgac ctcaggagca aagtcgctga ctgcttgcaa
    36001 tcgttctttc atcgtacttt tcatgtcatt tgtcagatat ttgctgtctg tacgcggata
    36061 agtaacgatc ttatgggtct catacaaact ttgtacaaga cttaatgttt tctttgcaga
    36121 atagccatag cgttgattgg cttctctttg gatctctgtt aaatcataag gcaatggcgc
    36181 agattccgtt tttaattttt cttggacttc tgtgacagtt cctttgcttg tactgatcaa
    36241 ttgaacaagt ttttgtgctt cttcttgtgt ttttaaagca aattgatttt tttgaagcat
    36301 tttggcttgt tcttttccag cttgcaacgt gatcgtgaaa tacgtttgtg gacggaactg
    36361 ctcgattttc ttttcttggt ctctcactaa tgctagtgtc ggtgtctgca cacggcctgc
    36421 tgataaatta tccgcgtact tcacagttaa ggctctggtc acattcaatc ctaccagcca
    36481 gtccgctttt gcccgggcaa gagcagattc atacaatgca tcatattctt tcgcaggtct
    36541 tagttgcttg aatccagttt tgattgcttt atctgtttga gaagagatcc ataaccgctt
    36601 cactggttta ttgaaatgca catactctaa gatccagcga gcaacaagtt ccccttctct
    36661 tccggcatct gtcgcaatga ctgcttctga gacatctttt cgctgagcca gctgcttgat
    36721 tgcttttaat tgatgtcctg ttttaggaag tggtttgatt cctagttttt tagggatcat
    36781 tggcaacgtg tccatttgcc agctctgcca ttccttattc agatcttccg gcattttcaa
    36841 tcctaataaa tgaccaaggg cccaagtaac gatcaccttg ggtccttcat aataattttt
    36901 gtgcttttga ttagcaccaa gcaccttgct taggtctttc gcaacgcttg gtttttccgc
    36961 tagaattagt tgtttcataa aattcctttc tgctttctac tgtgatgggc tgatgatttt
    37021 tcaacaacgc aagtcccttg tattgctgta aatcatcgtc acactcttca caccagcgct
    37081 catctccgtc attccaaaag gcgctgagaa agttactttt tgcattgata tattcataat
    37141 tttgatggat ttcgttccat tgtttttcgt acgcttgtcg tgcttcttcg aaagactcga
    37201 atgtttcttc ttcttcaata tcttcttccc agtcttcaaa aaaccaccaa ggctcattgt
    37261 ccccgaacat ggtaattact tgatacatac tcgtgctcct tctttcagct tttctagact
    37321 catcttagtc acttttcaag aaagttgcaa gaattttctc ttataaaagg gtcatttaat
    37381 gaacatctta aaattgttac aaatgaatct gattgtttaa ggcaaagtag cgataaagtt
    37441 attgatcatc caaaaatagc tgtcttctgg atcgattgtt ggttcttttg taaagtatcc
    37501 taaaaaacgt aaggaaagat aaccgtggat catactacga agttccctgc ttcgatgaac
    37561 tttttcttct ttcgttaatt gaaaagcctc aaatatctgc aaaatgatct gattcgtttc
    37621 atggattcca tctaataaaa cagcttcatt ggtatgcggt acactgatca gtaactcata
    37681 cactgcttga tttgaaaaag caaaggatcg ataagtttct gcatagatct gcagtgcttc
    37741 ctgaccactt tttcccacaa gagcccgtcg cagctggtca ttaagttcct gcagaagttt
    37801 cgctgttagc gctgttttga catcacgaat atttttgaaa tggttataca gcgagggagc
    37861 cttgatatcc aagcgtttgg ctaaatgctg aaaggttatt tgctgaacat ccatctcttc
    37921 tgctagttct cgaaagcagt cgatgatttt ctcttgagac agatttcttt ttttcatttc
    37981 catgtttcct ctcataaaat aaatccttca ctctttcagt ttaccacgaa aaagaaagag
    38041 aaaaaagaga ctttcattga taaactacat attgtagttt ataataaaga gctgtagatg
    38101 aaaattttca gaaagaagga gtctaattgg ctgcaagaat aaaggaaaaa ttatttagga
    38161 aaagagaaac ttgggaacaa aacttgtttg tcttatggtt tggaacattt atggctggta
    38221 tcggatttag tttagtcatg ccttttatgt cactttacat agatacgtta ggtaattata
    38281 gtacgtcaca attgaacttt tggagcggat tgactttttc atctactttc ttggtgacta
    38341 cgcttatctc tccttggtgg ggaaaattag ccgaccaaaa aggaagaaaa ttgatgctgt
    38401 tgcgtgcttc attaggaatg gcaatcgtca tttctttgat gggttgcgtg actagtgtgt
    38461 atcaattagt cgcattgcgt ctgcttcaag ggattttttc tggttatatc agtaatgcca
    38521 ctgcccttgt cgcaactggg acgccaaaag aaaaaagtgg tcaagttctt tgaaccttag
    38581 caactggttc tgttacagga acattacttg gaccgcttct tggcgggatc tctgcttcag
    38641 cgtttggcta tcgccctacc tttttcatta cagggatcat cttatttttc gtcttcttgt
    38701 tgagtctgtt tttcgtccat gaaaaatttg tcccagtgaa aaaagaaaac atggtttctg
    38761 ccaaacagat tttcaaagac ttgaaatatc cacacgtcgt catcggtatg ttcgtcacaa
    38821 cgatgatcat ccaagcatcc aacaattcca ttagtccaat catcagtctg tacattcgcc
    38881 aattgctgca tggacatggg aatgttacgt tgatcagcgg aatcattgct tcgatacctg
    38941 ggattgccac tttgatagcc gctccaagat ttggtcgctt aggtgacaag attgggagtg
    39001 aaagaatact tgcgattggt ttgggttttg cgattctggt ttatatccca atggcattcg
    39061 tcacgaatgt ttggcaatta gctgccttac gttttttgat tggtatctct gatgcttgtc
    39121 tgctccctgc tgtccaagca ttgatcacaa aatattcgcc tcatcaagct gctggacgga
    39181 tcttcagtta taaccaatct ttccaagcaa ctggtaacgt agtagggcct atgatcggct
    39241 ccagtgtttc cagtgcattt ggttatcgcg gcgtctttct ttcgacttct cttcttgttt
    39301 tggtcaactt cttgcttgta cgacacaata cacaagagat ccatcagcaa gaaactgcag
    39361 aagaaaaagc ttcaggagtc tccatagctt cagggcactc tccttctcat agacaataag
    39421 cgtaaatgaa aaacactgct tgaacagttg tcacagactg tccttgcagt gtttttgttt
    39481 aggcctgttt tttcaagaac tcagataaga ggaataatcc cttttccaag gtttcttttt
    39541 tacaacagta acctagtcgg gcatgcttcg gtaaatcaaa ggcacttcct ggtactaata
    39601 gtactcctgt ttcttctaat aaattgatac aaaatgtctg gtcatcttct tcgatggcta
    39661 atttaatgaa agaagtagag acgtaattgg gagcgacaag ttcaactttc ggttcattgg
    39721 ctacccattg ttttaaaatc acaaggttct ctgtaatgat ctttttgttt cgcgccaata
    39781 ttttttcttt attcttcaat gcatgtacag caagatcatc tgacaggact ccgccgcaaa
    39841 tcatcgtgta gtctcgatac tttcgaaaaa cttctgccaa ctcaggtcca gtagctgtcc
    39901 agccaatacg aatacctgga atggagtagg ttttggatag ggaattggtt gctatacctt
    39961 tttcatagac atctacgatc gacaagaatt cacctttatc cgttaaaggc gcatatactt
    40021 catctatcaa tacatatgca tctactgtac gagcaatttc tgcgattttt tctaaagttt
    40081 tccgatctag caatgtgcct gtaggattgt ttgcactatt caaacagatg atttttgttt
    40141 caggtttgac taaggcttct agctgttcta aatcaaactg ccaatcttct tcttccttca
    40201 aatgaacaaa gtcaacagcg gcacctaaag atttggggat gtcatataat tgctgataag
    40261 taggaagcat cgaaacaaca tggtctcctg cttccactaa cgcgtataga gctaaaagat
    40321 tagcaccagt cgctccattc gtttgtaaga tgttttccgg atcgacggtt tgataaagag
    40381 aagcgacttc ttgtttgaaa acatctgatc cttcgatcca gccatagtcc aatggttcgt
    40441 ttttgcgttt ttcgaaaaat ttacttacgc tagtcccatc taagccaacg agttcgtcta
    40501 atgtcaatgc ttcgatcgag ctttgtgcaa tatcatatac cgctttcttt tcccatttat
    40561 ttagccattc ttctacaccg aaatttgcga ttttcatgtt tctttccccc ttatatcttg
    40621 atccagccta cagcaagcaa cccgattgaa agcagtgcgg ccacacaaat cagcccaatc
    40681 attacttttt cttttcccgt aatttgatgt ttggtttcac gacaagcgat ccagtagaag
    40741 caaaaaccag gaagataggc aatactacat aaaaggacat aagaaaatcc tgctaaaatg
    40801 atgcccatca attcaaaaac tacagcaatc ccaccgatca ctacttgttt tttgattcct
    40861 tcttttaaag aaaactggac ttgatagatc ccgacaagta gataacaaat caaaattgcc
    40921 gctgtacata aagaataggc aaattgatag gcataatcag taactaagaa agtcaataag
    40981 aatagattag tcaagattgc agtaacaact aacgaataag ttggggcatt ttttttattc
    41041 gtttttcccc aacctttcgg caacagaccg tctcgagcca tcaacatcgt tgtttctgct
    41101 gggagcatcg tccaagacaa ccaagaacca atgatcgaaa taatcaagcc gatattgatc
    41161 agcgctgctc cccaatttcc cacgattcct tttaaaatat ttgccatagc aggttgtgaa
    41221 atagttgcta actcttcttg agataagacg ccgtatggca agatcgaagc aaggatatag
    41281 ataaacaata aacaaagcag accaatgatc gatgcttgct gggcttctga acgtttcttc
    41341 gcgcggttgg ctaaaacact tgcgccttcg atccctacaa agacccacat cataaccatc
    41401 aaacaacctt taatctgcaa ccagatcgtt ccttgctgcc ctgttccatt cactagatta
    41461 tcagatacgt taccccaaaa atcagcagtg aatatattca ctttaaatgc gataaataca
    41521 aatattaaaa ataggaataa tggcactaat ttacagatcg tcacgattgt attgatgacg
    41581 ctcgctcctt caactccatt gtttactaat aaagtcagac cccaaataaa aatacttgca
    41641 aataaaatag aaggaacatt ttgtccacct ttgaatatcg ggaaaaattc tcccatggcg
    41701 ctcatcagca tcgttgcaaa agcaacattt cccagccacg cagacaacca gtaaccccaa
    41761 ccactgatga agccacctaa tttaccaaat gaagcttccg cgtaaccgaa aataccacca
    41821 tctagatctg gacgcttttc tcctaaatga ttcagcgata ataccaaagc taaaatacca
    41881 atcccaacga ttatccatga aaggatcgct ggccctggag ctgcagaact agccagatca
    41941 cttgtgatcc caaacacacc gcttccgatt gacgaactga caaccagagc aaccagtccc
    42001 catttgctaa tacccgtcga tttcttttcc atttccatca ccctcactta taaaattgaa
    42061 caaatcactt gcaaaagatt cccttctatt ttgcaactgc tcttgcttaa caaaaagcaa
    42121 ctttattcat attattggat attatgaact ttttacacaa gtatttcaag tataaaatga
    42181 ataaaaatta attttattgc aaaaaaacag ctatgctatc caaaagaaaa ggtgtttctt
    42241 ttgttagcca tagctgtttt gattcattta atatcgctta aacgtattgt aaaacttttg
    42301 tttcttgata tgcgtggtcg atcaaaccac cacccagaca ttccattcca tcataaaata
    42361 caactgcttg tccaggagtg attgcacgta caggttcgtc gaatacgact tctgcacgat
    42421 tatcatccaa taagcgaact gtcacaggaa cgtcttgctg acgataacgg aatttcgctg
    42481 tacacttgaa ttcttttggc attggttcat tcgtcgtaaa gtggatttca cttgcatcca
    42541 agcttgtggc atacaatgca ggatgatgga acccttgccc cacatataat gtgtttgtcg
    42601 ctagatcttt tcctacaaca aaccagggtt cttgcgaatc tcctccgcca ccgatcccta
    42661 gtccttgacg ttgaccaatc gtataataca tcaagccagc atgttgtcct ttgacttcac
    42721 catcaagagt gaccatattt cctttttttg ctggcagata gttgctcaaa aattgtttga
    42781 agttcttttc accaataaag cagattcccg ttgaatcttt tttcttagca gtcgcaaggc
    42841 ctgcgcgttc agcaattgcc cgtacttctg atttttccat gccgcctaga gggaacatcg
    42901 tttttgaaag ctgttcttgt gataattggc taaggaagta agtttgatct ttattattgt
    42961 ctactccacg aagcatgtgg accacgccgt tttcatcgcg agtgacttgc gcataatgtc
    43021 cagtagctac ataatcggcg ccaagatcca ttgcataatc caagaatgct ttgaatttga
    43081 tttctttgtt gcacatcacg tctgggttag gtgtacgtcc tgcgcggtat tcggctagga
    43141 aatattcaaa tacacgatcc caatattctt tttcaaaatt cacagagtaa taaggaatcc
    43201 cgatttgagc agctactttc gctacatcct tataatcttc cgtggccgtg catacaccgt
    43261 tttcgtccgt gtcatcccaa tttttcatga atattccgat cacgtcgtat ccttgttcct
    43321 ttaacaaaag tgctgttaca gatgagtcga caccgccgct catccctacg acaacgcgtg
    43381 ttttgctgtt gtctgtcatg atatcaccat catttcaatt agattctgaa acatctacga
    43441 gttgaaggtc gcaacttttc agtattttac attatacgtc atatttctga aaatttcatc
    43501 ccttaattat caagtcgtaa aaatactgtt ctactggacc tattcagtac acaaacatga
    43561 tagtcaacca aaaaatcact catcttttga gcattcgata taaaaaattg gcatactcat
    43621 aaaaagggcc agcctttcgg caataaggaa taattttata aattgtgaaa aactattttt
    43681 caaaattgct tcttatcgct taaagctgca cgtctcattt acaacctttt ctttttagat
    43741 gttatgagtt gctcagataa gtgattttgt ttactctaag aaaaaaggga ggcaaaagat
    43801 acatgaatta ctttatcagt gatatgcact ttttccatga acgcttatta ggaaaaaacg
    43861 actttgctcc gcggctcttt gcaaatgtgg atgaaatgaa ccaacagttg atcactagct
    43921 ggaatgcagt aataaaagaa accgaccatg tttatcatct aggcgacctt gctatgcatc
    43981 cgcaatatga aaaagcaaat gcagaaattt tggaactggt cactcaactg caaggaacaa
    44041 tccacttgat caaagggaac catgacagtc gcgctttttt ccgttattta gaaaaaaatg
    44101 atcctaggct tactgacgga agtcccaaat tttattttta tgatgtaggt gccatcgtta
    44161 aattcgacca tcaacaatat tacttgactc attatccgat gttgctcggg cctaacgaca
    44221 ctatccgcaa tcttcatgga catatccatc attacagcgt acctatcgag aacgatgtca
    44281 atgtaggcgt agacgcaccg gaaagagaac tcttaaagga aaaactacca tttggcactc
    44341 cgttatctga gcaacaaatc aaagaaatct atgaagcaaa gaggaagtac gataggtcgt
    44401 gaaaaaactg ccctatatca agtattaaac aaataaagat aattactcat atttttcatt
    44461 tgagtaggct tattgccaca ggaacattag gatcagaaat ggagttgtaa caagacttct
    44521 gtcacaactc cattccatac tattcgtctt tttgctcatg gtgttctttt accagttttt
    44581 tctgtaattc tttatcataa tttcgggcag ctgacgttaa aaataaaacg attccgcata
    44641 ataaaatccc gattccggaa aacaagaaca ttttttctac ccctaaacga tcagctaatg
    44701 gtcctgcaaa taataatcca attggacctg ccaggttcat caaagagttg aagttcccta
    44761 agacacgacc taaaacattc gattcataac tctgctggat cattgccatc agcagtgtat
    44821 tgaaatatgg cgttgcacaa cctgctcctg cattcaagat aagaaagtac aagaaccctt
    44881 gactatcgtt tggaaccaat ccgcttgcac cgatcgtcag gcccataatg atgtaggcta
    44941 taataatagg tttcattcgg tctttccatg ttcctataaa actgatcagt gcaccgccta
    45001 agagcattcc gactgcgtag actacttcca ctgctcccgc ctgtccaacg gaaccaccaa
    45061 agtaatctag cgtcatcaat ggatacagac tgattgctgg catgaacaac agcatcacaa
    45121 atgcaccatt gatcgttatg taccataacc ctttattatc catcaattgc tgcagcccaa
    45181 attttgcatc tttaaaaata gtaagcggaa cttccaatat ttcaggagaa acttttggga
    45241 tcttcacaaa aatcaataag ccgacaccaa aaactgcccc caatacatct aataaaataa
    45301 ggtaattgac aggaacgact gaaaacaaag ccgcacctag agctggcgca atgatgaaat
    45361 tcgctgattg gaccattcct aactgtccat tcgtccttgt tatgtgagaa gatggaacga
    45421 ttgtcggcat aatcgactgg atcgttggca tttggaatgt ttgtgcgacc gaacgcataa
    45481 acaaagacac aaaaactagc caaatcggga aggattctga aatcgttccg actactgcta
    45541 aaataagcgc gaaaatcgct acaataatat ccgtgacaat caaaagagct tttttatccc
    45601 agcgatccac tagcggtcct acaaatgggc ttagtaaaac catgggaatc attcctagta
    45661 aagtagcaaa actcaaaatt gtcgccgaac cggtttctct tgtcaaatac cagatgatcg
    45721 catattggac gaccatacta gtgattcctg acaaaaactg tcccgataaa aagaacaaat
    45781 aatttctctt ccaattcgtt aaaacgaccg actgttcatt ttccataaac atccctcttt
    45841 tctttttcat tttgtatagc gtttgcacaa caatcattat acgggattct tttttcaaaa
    45901 aatacctttt tccctaaata acaatcgttt caatttaaat ttctttactt agaaacaata
    45961 aatcagcaaa atattaaaaa taacaacatg tgttaaattt aaaaataaaa aagacaacta
    46021 atttgcgtcg tccttacaaa gttagtcatc taacaatagg ccgtttcaaa actagttgtc
    46081 tttttatatc cacagatcat tcgacttttt cgaataaccc acggctaatc atcccatcca
    46141 tcagaaaata aaatttatct tggctcattt tctgttttgg atctttgaaa atattgactg
    46201 ctttcaatcc atttgctgta gtgatcgaca ttttcaatgt ttgaatatct tttgatacag
    46261 tgatttttaa tttgaatcct tctttgcttt gcggcgtggc atctaatggt cggatcaatt
    46321 gataattgcc tccgtttgtt tctgtaaatc cattatcgcg caatgtatat ctttttgcat
    46381 caggatgcaa acgatagcta attggtgcgc ccagtaccgt tgctgttgtt tcaaatgcca
    46441 tgattttcac ctcgataaaa ttagtttctc tttattataa aagtttatcc tgatttttac
    46501 taggtttttc agttaagttc cctaaaaaat gaaggcgttc tctcagacat ctgttcatat
    46561 aagaatgact tcactagtta ttttttgaca gcacgaacta attgatcaag gaaatactgg
    46621 atgtcgtctt cggtatttcc caaaccaaag ctgatacgga tggattcttt tagtatcggt
    46681 gtatcttctc catacattgc ttctaatacg tgtgaaggct cgatatttcc tgctgtacaa
    46741 gctgaaccag tagaaatagc gaccccatta agatcaagct tcatcagtag caaatcattt
    46801 gcttcgccaa ttaagcgaag attcaacaca tgagggagtt tattcgttaa atcaccattt
    46861 acttgataag ggatttcgtt tgcttctaat gtatcaagaa tcaattgctg gaatccttta
    46921 tatttgttgt tacgtgcttc acgttcttca ggtgacagca gttcaactgc ttttgccata
    46981 ccccagatcc ctgctaaatt ttctgtacct gcacgccgct tttcttcctg ctccccgcct
    47041 aaaagcaatg cagggatatt tactccgtct cgtttatata agaaaccgac gccttttggc
    47101 ccattgattt tatgagcaga gatacttaat aaatcgattc ctaattcttc tggatggatc
    47161 gtttggtttc cgtacgcttg taccgcatct gtatggaaat aagctggatg atccgctaaa
    47221 agttcaccga tttctttgat tggcatcaaa tttccgattt cattgttccc gtacatcaca
    47281 gagaccaaga tcgtatcttc tcgtaacgct tttttgacct cttcgatcga cagattccct
    47341 ttttcatcta caggcagata tgtaacgtca aacccctttg tctctaaata tttcatggta
    47401 ttaagaacag caggatgttc gatagctgta gtgattagat gtctgccttc tttttgacgc
    47461 gagaacgctg tttctaagat agctgtatta tccccttctg ttcccccact gttaaagatg
    47521 atctcgtgag gttttgcttg cagactgtct gcaatgattt gtcttgctgt ttccaatttt
    47581 tcatgcgcgt ggcgaccaaa tccatgtatg cttgaaggat ttccaaacgt gtcttgcatg
    47641 attgccgtca tcgcttcaat tacttgtgga tgcatggggg tagttgccgc atgatccaga
    47701 taaatagttt tcacttcatt catcccttcc aaattattgc taggcacatt ctagcataaa
    47761 atagcgaaca aaccaaagaa gagctcattt ttgtctagag gacgtttcat gggcatagaa
    47821 aaaaggagct gtgacaaaag tcttgtcgca gctccttgaa gctgctcact tctgtcacaa
    47881 cttctttccc tctttttttt gtttattcga attctttttc ttccagacgg aataacggac
    47941 tcatcggtgt attttcatgg atgcgtttga ccgcttcacc cattaattct gaacatgtaa
    48001 cgattgttaa gttttctgga tgacgatttt cttctgtgaa aacagaatca gtgatacaaa
    48061 tatctttgat tggtgcattg tctaaaatac ttttcgcatg atcggacaat aatccatgtg
    48121 acgcacaggc atatacttct tttgcgccgt tttcttttaa tacatttgct gctcgtgcaa
    48181 gcgttgctcc tgtgttcaag atatcatcta ccatgataca tgtctttcct tgaacatcac
    48241 cgatcacata accaccctca gttgaagcat catctgcatg atctacgatg gctaatgtgc
    48301 tgttcaaata ttctgaaaga ctacgtgcac gctgaacacc gctgtttttt ggcgaaacaa
    48361 ctacgatgtc atctcccgtc agacctaatt gacggtaata atgagcaaac aacggcatcg
    48421 taaataaatt atctacagga atatcaaaga atccttgtac ttgaaccgtg tgcagatcta
    48481 aagtcagtac tcgagttgcc cctgcttctt cgatcaagtt agcgatcaat ttcgcagtga
    48541 tcggttcatg aggtttcgcc gtgcggtctt gcctagcata cccatagtaa ggcaatacga
    48601 cattgatcgt tttcgcactt gctcgcttca ttgcatcgat catgatcagt agttccatat
    48661 agtaatcatt gacaggtgca ttcgttgatt gaacgatata cacatgatcc cctcgaatac
    48721 tttcttcgat attgatggaa atctcgccat cactaaattg cttcacgacg cttttaccta
    48781 attctgttcc gaacactttc gcgatttttt cagctaatgg acggttgcca ttcaaactaa
    48841 aaatcttcaa cgtgtcatcc tgatacttat tactcatgta gtcctcccca aaacttctct
    48901 attcttcgat agtttaacat atttttccaa gattttctgt taatttcagc aaattcttaa
    48961 ggaagtgaaa ctgttccgtt actccaactg gcaggaatga ctttatgata atcgccccag
    49021 ttgtcgtgta ctttgtaata acgttctcct gtttcagttt caaaataagc gtacgctgtc
    49081 acccaatgat tgccatatgt actgccaagt actttcaaaa taccaatcat cacaggtttg
    49141 ccttcacgta tacgttttgt agctcgctgc catgaaccta gaagagttgc tcgtgcacgc
    49201 tcatgattgc cattcttctt taaaaaacta gaaatccctg tactaacctg aaaaggaatc
    49261 gtaggaagtc ctagtggttg gatctggcta cgcaatgcct tcgtcaaaac caacgaatca
    49321 gccgtatttt tcttgcgaat ctcatttgga agcatccacc ccttacgata gtcttgataa
    49381 taggcaagga gaactgctgc cgcgtaagta ccacaaagat agcctgaggg ggtagcccat
    49441 ttcttatacc tcaaaaaacg ttcttctggc aatcccaccc agcaatttgc gagttccttt
    49501 cctgaccttt tttcttcgat caaaacggga atatcttctt ttctgttgat ccatctagat
    49561 aactcgattg gctcttcatt gatcagcttc cattttttac aagaaagacc ctcttgataa
    49621 gtatattttg ctggagaata ttctaccgga tgtcctgatt gatcgataat cacataactt
    49681 tgatcttcaa aaaccagagc taaaaaaagc aatttacttt ttcttgactg cagctgctga
    49741 gtgctggcta attttttaat ttttttatct actttgaaat ataaaggttt atccactact
    49801 tgattcagtt gggatcgcct cctttttttc attataagtg aaggattgaa aaaaacaaat
    49861 cagtttgatt gttcgaattt tcaatttttt atgttctatt tgttaattcc ttcattttgt
    49921 aagataatgg gcacacgaaa caaaaaagga gcgatcgatg aatgaacaaa gaacgattga
    49981 aacaagattt ctatgaagca gtcaatgaag aatggcaaaa aacagcaaaa atccctgcag
    50041 ataaaccagc tacaggcggt ttccaagatt tagttgacgg gatcgaccaa cttttgatgg
    50101 gagaaacaga cgatctgctt gcccatccag aaaaagtgtc ttcagaaaaa atgaaacatt
    50161 tccttgcata ttatcaactg gcaaacgatt atgaccgaag aaatgagttg catgctcaac
    50221 cgcttcaacc tataatcgaa aaagttgaat cattgcagtc tttcgacgaa ttgaataagc
    50281 aatttccaga atggctgttg gatacattac cattgccatt taatctggat gtagatgcag
    50341 atatgaagaa tgcgcaaacg aacgccttct ttgcttatcc gccttcttta ttccttccag
    50401 ataagacgta ttatgcatca gaccatccca acggtgcaca actaataggc gttttctttg
    50461 atatggctgt tcagttatta gagcttgccg gcaaagaaaa aagtcaggca gaagcaatca
    50521 cggaacaagc agtgcaattt gataaattga ttgctcctca tgtaaaaagt gcagaagaaa
    50581 acgcagatta cagcaaaatg tataatccgc aatcttttga tacatttatt aaatacacag
    50641 ataaactgga cttgaaaaaa cttgtcacgg gtttgattgg gacaactcca gataaagtga
    50701 tcgtaactga tccagtgtac ttcgatcatt tgaatgacat cctgacagaa gctcattttc
    50761 cgttattcaa aagctggatg atcattaaaa cagtacgttc tcttagtggt tatctatcag
    50821 aagaattccg ccaagtcagc ggaatctatt ctcgcgtatt gtctggaaca gacgaagcaa
    50881 tgcctcagaa aaaagcagcg tattaccttg cctctggaca attcgatcaa gtagttggcg
    50941 attactacgg aaaaaaatat tttggcgaag ctgctaaaaa agacgtagaa caaatggtga
    51001 aaaaaatgat cgccgtttat aaaaaacgtc tagaagaaaa tgactggctt ggggatgcaa
    51061 caaaagaaaa agccgtcata aaattaaata acttaggtat ccaagtaggc taccccgata
    51121 agatcccagc attattcgac cagttcaaaa cagtacctgc agatcagggt ggtactttgc
    51181 tttcaaatac tcttgttttc agcaaactca cattaaaaga tcgtttcagc aaatggaaca
    51241 aaccagtgga tcgttcagaa tgggaaatga gtgctgacac ggtcaatgct tattatcatc
    51301 catttcggaa tgtgatcgtc tttccggcag cgatccttca agcaccattt tacagtctag
    51361 aacaatcaag ctctgctaat tatggtggaa tcggagctgt tatcgctcat gagatttccc
    51421 atgcatttga taataacggc gctttatttg acgaatatgg caacttgaac aactggtgga
    51481 cagaagaaga tcttacacaa tttcaaaaca aagcacaagc aatgatcgaa gaattcgatg
    51541 gtctgccttt tgctgacgga aaagtaaacg gaaaattaac cgtttcggaa aatattgctg
    51601 atgcaggcgg tctaagctgt gcattggaag cagcaaaaac ggaatcagat ctttctatcg
    51661 aggaattctt tgtcaattgg gcaacgattt ggcgtacaaa agcaaaaaaa gaataccaac
    51721 aacttttact gcaaatcgat gtccatgccc ctgctaaact ccgagcaaac gtgcaaccaa
    51781 aaaaccttga cgaattctat gaaacatttg gtatcacgga acaagacgct atgtatctag
    51841 cgccagaaaa aagagtgcga atctggtaag aggttgtgcc agaagtggtc agcttcaaga
    51901 aataagaagg aatttgggaa aatcgcttct caaatttttg tgacatttcg gcttattttt
    51961 cgaagaagtt acttctggaa taccatgtat tagaggttgt gaaaaagctg cttagtttca
    52021 agtagtaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttact
    52081 accgagaaac tggcttttga acaccgttta tcgagaggtt gtgaaatcag cgtcctgacc
    52141 tgagtattag agaaaaaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
    52201 taccgagggt cagctgattg aacaccgttt agtggagaga ttgtgaaaaa gctgtcctgc
    52261 atcaagtaat aagaacagcc aatcaaaagc agcaaggaga acctcatcta taaaaagata
    52321 gaaggaagtg agacatctat tggtctcatt tccttctatt tttactttcc tcagtcaatt
    52381 gttttacatc ataaaaggag aaattccact tatttttttc gctgtttcat gacataaaca
    52441 actacaccga ttaacaaaat tcctccagct aatgctagca acgggttatt aaattctccc
    52501 gtttttggga gtccttgagt gccatttttt tcactttctt ttgctgtcct atccggatga
    52561 gaaggttttc catttggttt tccatttggt gattcatttc ctggattttc tctttctggt
    52621 ttgtcatttc ccgggggcac atcggcagtt tctcgcacag tgatcgcttt ttgctgggta
    52681 acagattctg tatgagcaga ccatttttcc ccatcaaata tttccgaacg ataagtgact
    52741 tctagcgtat attctccttt tgaaggtgct gaaaaaatag cggtatgatt agaaaattga
    52801 gcacttgact gtgtttcaac gatttggatc ttttctggga aataacgcat atcttcaaag
    52861 acaggcgttc cttgatttgg tgtgaaagtt gacacaaaaa ctgtaaaagg cttcagttca
    52921 atgatatttc cttctgcatt ctccgccaca ccatttgctt cttgtataat aatcggatta
    52981 ttttgttcat ttggttttgt ctgatttggt tcttgtacga ttgcatactc tggatattcc
    53041 gaagacggtg aggtttctcc gtcaaaaata gcacctacag ataatggaag accatccact
    53101 ctttcctttt ctgtttgacg attccccggt ttaatctgca tcagtccatg aacatggata
    53161 cttccatttt catagcgcgt aacaggctgc tgaccttctt tcgaacatgt atttagagac
    53221 tcgatccaat cttttgatat ggtatgtcca agagtatttt ctgattgttt ctctcgttca
    53281 ttccaatagt agttcaacga gtggtagatt tgatcttcgt tttgattcaa tgggcggatc
    53341 tgctctctca tctcaagatt ctctgttcgg aaagataaga taccagaagc atggtagtct
    53401 gtgtttcctg ctgaagaggt ataaagagcc acattatatg aaccgttatt aaaacttgta
    53461 ctgctattga cgataatatc tgttccgcta ttggaatcga ttcctttcgc aagattttcg
    53521 aaagacaaac tattgatcaa ttcatgaggg cccgttaaag aagaaccgcc cattttgaat
    53581 ccattaccgt taccttctcc ctcaattcct ggtacccagc cgtttcgata agcaactgag
    53641 tttttgatga ttacttttcc gatcgaacct gtttcatttt tagcaaaaag gtcccaaccg
    53701 tcatctgcat tatgatacgc gatacaaccg tcaaatacat ttccttctcc tattgtcagt
    53761 ttggccgcaa atccatcggc atcttcaaat cctggatcgg cattttcaaa agatgtacag
    53821 ttcttgacta agttatgcgc gggccatgct tcgaaaggat catttgagct accgctgatt
    53881 tggattcctg tatttccatt ccgataagca tgaatctctt cgatcaaatt atgatttcca
    53941 gaaatctgta atcctttttg catagctgca ctatttgtaa tgtcaaaccc tcgtaaaccc
    54001 cagtagtgac tgaacagtgt catcccattt ccagttcctt taaaatcaag tacaggacgc
    54061 ttgttttctg gatttttctc tgcaatcaac tgaatcgggt tttctcttgt cccgttaacg
    54121 ccttttggaa tcgtcaatcc tctttcgaag gaataagttc caggcgccat gacgattgtc
    54181 tgcccagcat atgcgtatct caatgcttgt gtcagagata atggattttg tctgcttccg
    54241 tttcctgcat ctgacccttc tggtgttaca taaactgtcg aatatttagg tttacggtag
    54301 tccactgtat gaagaatctc ttttgtttca taactagtca tctctacgta ttcttctggc
    54361 ggatagtttt tatctggcgt aaaactgaaa gtaaattcgt tcgttccttc catcagtttt
    54421 gtctgataat caaattcccg attcgcagtg atagccaagt tttttgcgtt cttaagcacc
    54481 tctcctgttg ctttattttt aattgtcaat aatccgtcag catttgctct aaaggacata
    54541 gtatgatctt ggtttcctga atgattacta gaggtcacag tgtaaatcgg atcaatcaag
    54601 cgagtaggtc tttcttgccc aggaggatct gtcgcaggat ctgagtaagc aacttcgatt
    54661 ccttctacct tgatcgtcat attccttgct gcagcaaatc ctgcataaat aaccgattca
    54721 tctgaagcgt ataatttttc ccagtcatac atgatgtcag aagccatttc ttctcctgtt
    54781 tcaggatcgt aataatatgc ctctaaacca gtattcgttt ttttcatctc tacgtccagt
    54841 ttagtaatcg agttcgtggc cggaaacaga ttcccatgac cgtttccgaa tatattataa
    54901 gaacctgttt ctttttccaa gaaccctgct gaagtctcta aaggtgttgt ttccactgag
    54961 acactcccag gagctggcgc agcttgcgga tcttcagatg atattcctgt gattgcccgt
    55021 gtccccaatc caagtcgcat cgtatatttg ctgccgctcg tgttgaccac ttcttgtcta
    55081 tccgagtccc agacatattc gatccgactg cctagtatgg caaatgaatt tgaataaaat
    55141 gcctgcccaa acaaatgatt ttttggtact gcgtctctta ccattaaagc aaatccttct
    55201 tgggcattcg tataggtcca agattctatt tcgatcgtcg cccgcatcct aaagttgatg
    55261 tctttgggga ttgcttgata ataataggtc attccatctg atccgcttga ctggaatttt
    55321 ccgccgttat tcgtggaaga gagccgcacg ccgtcttcta atggttcaat ggcataccta
    55381 ctagcacttg tgctttctcc gaaaacaacc cctttccact cttcatttgc ttcttcagat
    55441 tgcttttttg tcttttcatt ttctgattct ttcatcatag gatcatttcc ttcttccgta
    55501 tccatatctt cactagttct tggtatttcc tccattgttg acatcccttc aatgttcacg
    55561 tgtgcatttt cttcgttcac agactgttgt tcgttagcag caacttcaac gacagaaatt
    55621 aaaaccgggg tactgagcat aaggagattc atcattagcg cactgtattt tatacgtttt
    55681 tttaacaagt gtattcctcc cgtaacaaat tagaaagcgt ttacttaaag tattacctaa
    55741 tttattttta ctgtcaataa aaaacaaaat tataataagt gtattttata aaaagaaaaa
    55801 atataactta agttgttgat aataaagaga tttattaacg gaaaaagaat aagttttttt
    55861 aaaataacgt ataaaaaaga gcataaaaca aagtcgtttg ttttatgctc ttcttttatt
    55921 attttgtgct ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt
    55981 gtggactcaa aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggaa
    56041 tctttgaaaa tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca
    56101 cagatatctg tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg
    56161 atgtctagat ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt
    56221 tcctcttgct catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc
    56281 gttcctatca cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga
    56341 tcatgcgcat caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga
    56401 gcagtcagct ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct
    56461 gcaacaaaat catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata
    56521 cgagatgttt gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa
    56581 attcgtcaaa gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca
    56641 aaaatgggat attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag
    56701 ctaatgcttc agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg
    56761 tatcacctgt aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa
    56821 ccatttcact gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc
    56881 ctgggttgta gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa
    56941 cgatgtcacc tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca
    57001 tgttcacgcc gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta
    57061 taccggaacg tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc
    57121 ctgcgcgttc ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt
    57181 ccattgcgcc gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga
    57241 ttgctaaaaa aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag
    57301 agttccattt ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct
    57361 ttttgctttc tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt
    57421 ctgcttttcg ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt
    57481 cgcttcgcgt aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca
    57541 atagttccca gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca
    57601 tcgcttgttg cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt
    57661 gtgctaaagg attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat
    57721 ttgcaaaacc tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc
    57781 cagtttcttc ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat
    57841 cgatttttcc agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga
    57901 caagaagcag acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata
    57961 ccagttctct cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa
    58021 taatttttcc gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt
    58081 tcagcatatc ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt
    58141 tttctcaaaa actccttatt agtttaccat agataaacct tagattttct ctaaatatag
    58201 aaaatccttc tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa
    58261 tcgaataacg aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata
    58321 aaacgaaatt ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat
    58381 agtttttctt ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc
    58441 tttggttcgc gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt
    58501 gaaactcact atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca
    58561 atgaatcaaa caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa
    58621 cttaaagcaa tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa
    58681 ttggtgaaag aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg
    58741 aatctagttg atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa
    58801 cagacctatg aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc
    58861 gcacaagatt atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct
    58921 gttgcgaaac agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc
    58981 ttttgttcat aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt
    59041 ttctaaagta gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt
    59101 atggatcata aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat
    59161 ttattgaaca acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag
    59221 attgatcagc tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa
    59281 cgccaagaac aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc
    59341 atcagctacg gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat
    59401 catgtgcaag cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt
    59461 ctccaagaag ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc
    59521 ttcgggaagg tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca
    59581 caaattgaca agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat
    59641 ctgatgttag aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat
    59701 atcgctgctg gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg
    59761 aaaagagcag aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa
    59821 ttcctagatc gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc
    59881 cagcaagcac cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc
    59941 caagcatcta tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc
    60001 ttgcttcgtc aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac
    60061 ttattgaaga aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat
    60121 gaacgtggaa tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca
    60181 atccaagaga cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta
    60241 gagcttggac acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc
    60301 acctttcgct tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc
    60361 gtttttctta tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt
    60421 gtccatagcc atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga
    60481 tccatgtcac agtttgcaga cgaagcatca atacaataca agtcaagatg gagacgattg
    60541 gcaatagcgg aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc
    60601 tcaacttcag taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta
    60661 gttccgctaa tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg
    60721 cataagtcgc ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa
    60781 gtaagccatc cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg
    60841 ttaccgtcag tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc
    60901 ctacatacct cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga
    60961 ttcctgtcaa gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa
    61021 tgattcctct tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg
    61081 catcaaatcc caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac
    61141 caaaaggcat gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa
    61201 acaacgcgat gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct
    61261 tggcttcaag cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa
    61321 tgtatgtacc attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta
    61381 aacttttcaa aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta
    61441 gtaaaaattc cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa
    61501 catacagata ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac
    61561 ttagtccaga caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac
    61621 cagcattttg attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac
    61681 caattccaag catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat
    61741 tgagattgat ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg
    61801 aaaacagcga ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa
    61861 ggcaaccaac aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa
    61921 tcccacctaa agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac
    61981 caagtgattt gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga
    62041 tcatgttttt ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc
    62101 ctaagatcat tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt
    62161 tgacgatgat ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct
    62221 taccaaaatc aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag
    62281 tcaaatacgc accgcttaca agaagagcta aagcaataat accgtataca taatctttcg
    62341 tttgtgtcca ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac
    62401 cagtagacaa tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat
    62461 tttgtccaac actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga
    62521 ttacaggact aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc
    62581 ctagtgtttg ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc
    62641 atgccaacat agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca
    62701 tgataagaaa cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg
    62761 attttctaac gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc
    62821 ccttttcctt cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt
    62881 gctaaataaa gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta
    62941 aatcccttat caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta
    63001 actgaaaggc tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc
    63061 ggcgtatatt tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca
    63121 tcggtcaaaa cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa
    63181 taatttgcct gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg
    63241 aggtagtccc gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca
    63301 tcgatttttg ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg
    63361 atcgaacgaa ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag
    63421 tcaatataat ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt
    63481 tcacggatca gctgattgat atattcttct accagttttt cttcttctgt cctcccacca
    63541 ctcaaataat tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt
    63601 ctcttgctca agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct
    63661 aaataagcaa ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc
    63721 gtcaatgcaa taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta
    63781 aaaaaattca tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg
    63841 tttcgatata tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga
    63901 ctgtcaatgt attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt
    63961 cccgacttaa gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt
    64021 tgctcaaatc aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa
    64081 tgccattgtg gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta
    64141 atgcattgat ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg
    64201 cccccatgtt catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga
    64261 tcggtacttt cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa
    64321 gattaatatc tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat
    64381 tgaaatcggt cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt
    64441 gtcgaatgat ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct
    64501 ttcttcttgc tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa
    64561 atgtcatttt agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc
    64621 ttccaagcag cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt
    64681 tctttcgttg tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca
    64741 ctagcaatat acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt
    64801 ctatctttga agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt
    64861 gagtgttcac ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa
    64921 ttgctaatgt atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga
    64981 gcatccgacc atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg
    65041 taaagagttt tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc
    65101 ctccagttgg aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat
    65161 tcgtttcatc aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca
    65221 tatgttgttc gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag
    65281 gatctttgac ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct
    65341 tttcatgcac tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt
    65401 gataattctc gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat
    65461 atgtaaaaac tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac
    65521 tgatttgggt ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta
    65581 aaaaaactgc agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact
    65641 tcagacttca ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg
    65701 attagtaaga atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata
    65761 ttcggtactc tttctggccc tggattttct actcctacga tctgaatttc ttcattatag
    65821 cttccataag agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct
    65881 tctggcgcca tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag
    65941 tatccatttc catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta
    66001 tctgctcctg attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca
    66061 gaacggtctt cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca
    66121 tcatattttt caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct
    66181 tcataatcaa attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca
    66241 tccgcttcta gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt
    66301 gcatctgcta atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc
    66361 gtcacgacaa acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc
    66421 gtctcgccgg ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta
    66481 tctgtgattt tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa
    66541 taaggaatag gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa
    66601 gaaatcgttt cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc
    66661 tctacattca tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct
    66721 gtattgtatg tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc
    66781 atcactggca tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa
    66841 gtttctaaga aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg
    66901 gtagagaata ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga
    66961 tattttaaaa accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa
    67021 ttaagaccgt cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact
    67081 tccgttacgt caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta
    67141 gaaaccaatc cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat
    67201 gtaatcgtca cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc
    67261 ccgaaaatcc ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt
    67321 tttttattca tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa
    67381 aagaattcct agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca
    67441 ttttcctgat tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc
    67501 ttttacggtc tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt
    67561 cccggattct aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt
    67621 tgcatccacc caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc
    67681 gtaaccttcc acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg
    67741 tcctgtcgta acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa
    67801 aggatcattg tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc
    67861 taaataatat ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg
    67921 ttttcctttt tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag
    67981 ataaccgaca tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg
    68041 gtgataaatt ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa
    68101 tccaacttct ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt
    68161 tttcttaggt gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc
    68221 agttgttacc gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt
    68281 gtttctgttc gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc
    68341 ttttttttca tgataataat cggtcatgtc gtaaatggca aatgtcgctc catttagtgg
    68401 caccgtctgt gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc
    68461 attttcatgg tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa
    68521 cacgaattcc acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac
    68581 tgctgcaagc ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac
    68641 cttgctccct cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc
    68701 atgaagaatg cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac
    68761 ggattttcag gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt
    68821 tcatattcta actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa
    68881 atctcaactt ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca
    68941 ggttcttctc gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca
    69001 ggtgacttca cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg
    69061 gaagaatcgt ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta
    69121 ttttgtttct caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt
    69181 ttcaaaagtt ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca
    69241 tcgatgattt catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca
    69301 atgataaact taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac
    69361 ccatttggtg cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg
    69421 tcgtaattta aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc
    69481 ccaggcaaac gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc
    69541 gggaaggaag aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct
    69601 tcatggtcta attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat
    69661 gctacccata aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca
    69721 cctgtttgct tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg
    69781 tcattttcat caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct
    69841 gcatagttgg tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga
    69901 tgaataactt tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac
    69961 gtgagtctcc ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga
    70021 tatccgattt ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt
    70081 ggcatattac tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt
    70141 cgcatttcca gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt
    70201 acaacagaaa gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt
    70261 ccgccgactc tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca
    70321 tcttcgttgg ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa
    70381 cgtggtggtc cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct
    70441 cctaaaatcg gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt
    70501 tcgattggtt cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca
    70561 gccaacggct cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg
    70621 taacgaatgg aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc
    70681 tccaggttct gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc
    70741 gcatcaagtt ttacttggta cttgatcctg accatttccc cttcacctaa agaaatcgga
    70801 gaaaatttat aagcttggtt ttcccgtccg acatacgttg tcggagtcgc cactacttct
    70861 ggtggggctt gtccatgacg ttcttcatcc catatttctg gctcttggta cttcatgccg
    70921 ttttctcctg gatctagatg gtaaaactga atatctgccg atctgccagc tccaaatggt
    70981 gagacattaa aaccagtttc atcataaagt gtggcttgct ggatcgttct cttgaaggta
    71041 gaagagatat cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc
    71101 ggtgttacgc catcttcttc taaatcagca atatttttca atacatttcg tccaatagca
    71161 tctagtcgtt gtctcccgct aacgtttcca tcgattccta aaccaatgga gaagatactt
    71221 ggcgggttct cttgatactt atttttcagc caatgatgat aagcaactgt gttgatcgca
    71281 gatgactttt cttgcgaagt aggactgcca ttcgtattgt tattagaaga accatcacca
    71341 acccagtgag tcgctttatt atctccttct tttacctctc cataacgata gtgtacattt
    71401 acttcatctt ctggacggtt aaagtcagat gcataggtaa ctggatgagt aaaccctgca
    71461 ttagaagtat tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca
    71521 atcttatctt ccatcacagt ccagttgctc caagctccat tagtgctcgt acgataactt
    71581 gactgaatcg gtgcataact gaaggtcggt gtcccatctc ccacaacgat taggattttt
    71641 tctggatttc tattttctcc gccattgtct ttatacaatg tttcatagcc atttttaagc
    71701 cctagtgtaa gcggagttcc tccgattggt gtacgattta ttgctgtatt ctcacttccc
    71761 gtcaaacgat tgatatcatt tgtcaatgga aagatgtccg aactgatttg ctcatggtta
    71821 ttgatagctg tactattatg gtaaaaattc accataccga ttcgaatatc cagactcggg
    71881 tcagagagca gatctttact catttcgttt actgcctcaa tcaacgcagc atttttagtt
    71941 tgactattgg tcccagcggt taattcactc atactcgctg acttatctag aacaaaaacg
    72001 atatcgattg gactgatttc ctttttctcg cccccgatga catctagata gacattgtaa
    72061 agggttgttg catcttcagg atgtagatca gatgctgtgc tgcttggcag aacaggttct
    72121 acccattttt tgatgtacgc cccgttataa tccaagtaac tgttttcaaa attccgttca
    72181 tttccccaaa gctgaacggt ttgagctgga acgtttctat cttcttcgcc tgcagttgat
    72241 ccattattag tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt ataatcacta
    72301 tcatcgatca agttctctcc gtcatatggc gagcctttgt ctccttgata gcgttcattg
    72361 ctttgtgtcc aatttcctaa cacttttgta tagtcattcg ttgtaaactt cgggtagcga
    72421 tgctgttcgt tttcaacttc tccgctagga tagatatatt cataggggtc ttgcgtagct
    72481 gttggcccaa gacgctccaa aataccgttg aataactggc gttgaatgcg ctggagtgct
    72541 tgaccgctgg gtacgattgg ttcgtccagc ttttccatct cattttcttc gctacgttga
    72601 atttcagcgg aatcttctgt atcagtttca ttttcttgta attcttggtt atctattctt
    72661 tcatcaacca ccacttgaag aggtagttac ccttcgttca catcggggat cttttctgtt
    72721 tcaaaaatca atgtgccggt ttctgcttct tttgaataaa cttttccgac ataccattct
    72781 tgaccatcga tcgtctcgac ttttaggtct tctccatctt catcatagga ggatagacct
    72841 gttccacgaa cgttttttac ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt
    72901 attctacctt cattggcact ttcctttttt tcaaaagcca gatgccattc aagggtttct
    72961 tctttttcaa tatacttccc ttcaattttt gcatagtcct catctaagaa tacctcatcc
    73021 tcctttgttt ctgttgactc agcagctaca tccagggtcg tcatatgata gactgactgg
    73081 aaatatggtc caaatagcaa actgacagtt actaactgca aaagcttcaa ttttaaccct
    73141 ttcacatcct tatactcctt tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca
    73201 atgcccatct aattataatt taatttaatt ttaattttaa atcaaatgat atccttttta
    73261 gttaaaaaac accaattata caacatttat tattaaaatt aaattataaa attttataac
    73321 gttctcattt aacaaaactt aagttattaa gacaaatgaa taaacaacta cgctttcctc
    73381 gaaacaaaaa aataaagcac aaatgcgcat ttacgcactc atgctttatt cgtacaatta
    73441 attcagataa cggcagcgca taggatgcca ataacacttc ctaaaatagc tcctcctaaa
    73501 atgtcagtag gataatgtaa aagtaaaaaa atcctcgaat aagcgatcaa acaagcaaat
    73561 aaaaaggcta atattcctgc aactggactt aattcgaaca acgcagctac tgataaaaag
    73621 gctagcgctg tatgtcctga aggaaaagaa gaagtcttcg gtttacttgt gtaaaggaca
    73681 agccgacgat ttttttcata aggtcttttc cgtcttgtcg tttttttgat aaaaagcaaa
    73741 ccaacaaaaa aatagatcaa actgccgata agaatggcta gaccatattc tctagtatca
    73801 ggtatcaaga ctaaaacagc agcaaggagc aaccaaataa aaccaccatt agagatatta
    73861 ctgataaatt tcaaaaaatt gatttttcga ctatccgttt tccgttgctt ggcgaacttg
    73921 ttgataatac tatcatcgat tttagcaatc aaacgcaaca tgttattcct cctttttaag
    73981 aaccttttct tgcgtacgat ggataaaaag caggacttga aaataaaaca agaaactgaa
    74041 acctaataaa gcaaaataga ttggtcctgc aaaccagaat gcgacaagaa ggatcaacgt
    74101 gcctgccaaa tagaaaagcg cgcttttgaa atcattaaaa aacaagaaaa atgcagtaat
    74161 ccaagaagct tctttttgtt ccagttgaag caagattttg taactaaaga gaagcgcgct
    74221 taatagaagc aaaaacaacg tagtatacat caatgtatag ccaattcccc caccccaata
    74281 gcggaaaatc tgataatcga tcaatagaag aagaaaataa acacttgcta aactatcttg
    74341 ctttccataa agagagaagt tctcacgaaa ctttttccag aatagtgaac cgaacttttt
    74401 ctcttcattt tttttgtaat gataaatcat atacagaact gttcgacttg cagttcctaa
    74461 tgtgaaaaca ggcaagacgc acaggaacca tagtagctgt aaatggataa gacgtggaat
    74521 ccattcaatg atttctaaga aataatcatt ggctttttta ccaatcgatc gattcatcat
    74581 ccttttgaac ctcctgtcaa atccatccct tcaatgaaat aacgttgtgc aaaaaagaac
    74641 agcaaaatcg tcgggatcaa gacaagtgtt gctcctgcca tcagataatt ccattgtgtc
    74701 gttgcttggt tctgaaaaac ttgcaagcca atctgtaacg tgtatttttc ttgatcttgg
    74761 atatataaca gaggacctaa gaaatcattc caagctgcgt tgaaggaatt gatcgcaatg
    74821 gtaatcaagg caggtttcgt caatggaagc atcaaatgac tccaaatata caaatgattc
    74881 gctccgtcga tttcagcagc ttcgatcaat tcattattga tcgataagta aaattgacgc
    74941 atcagaaata tattgaacgc attgccgaag aacgatggaa cgatcagggg caaataagta
    75001 cctacccagc cgatcttgga gaataataca tattgaggga tcatcgttac aaaccccggg
    75061 atcatcatcg tagaaagaac caaagcaaac atcagctttt tcccaggaaa atcaagcttc
    75121 gcaaatccat aagcaataaa agaatttgaa aggacattac cgatcactac tagcacagtg
    75181 atgaataatg tatttcttgc atagcgccaa aaaggaaagg cggcaatcgt atccagatag
    75241 ttttcccata cgattttttc tgggaaaaag gtcggaggat aacgaacgat ttcttccatt
    75301 ggtttcaatg aagtaaacac catccaaagc aatggaatga tcaaaatgat tcctattact
    75361 gtcatgagga cataacttag tatttttttt gtttgccgat tcacactggt ctttttgaaa
    75421 taatccatca tttgccgtca cctccagaat aatagaccca cttaggtgca agcttcaaat
    75481 tgacaaacgt cagaaccaac acgatcagaa acaggatcat tgacatagcc atcgcataac
    75541 ccatatcaaa agctacgaac gccttgttcc acatatgcag attataaaaa agcaaagaat
    75601 ttgcaggtcc gcctgttcca tttttcgtca tgacataagc ttcttgaaaa atttggaaag
    75661 aaccgatcgt agaggtgatc acatcgtaaa aaataatagg agtgattaat ggcaaagtaa
    75721 tacttttgaa ctttccccaa acgcctgctc catcgatttc tgctgattca tataaagatc
    75781 taggaacatt ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa ctccatattt
    75841 tcatcaaaat caacgcaggt ttcgtccatt gtggatcaaa cagccaagct ggtccaggga
    75901 tatggaacca gcccagaaat gtgttgatca atcctgcaga tggagaaagc agctgcatcc
    75961 ataagatgta gactgctact cctgataaga ctgccggtaa atagaaagct gtacggaata
    76021 cactgattcc tttgattttt tgattgagta ctgtggctaa aaacacactg aaaatcgtcg
    76081 tcagcggtac attgaagatg acgaaataaa cggtgttgta taacgatttc caaaataaat
    76141 catcgtgaac gaacattctt ttgaaatttg ctagaccaat gaaattcatc ttggatgtca
    76201 aattataatc ggtgaaacta ccaataactg aaaaaagcat aggccctagc ataaatacca
    76261 agaacccgat aataaaaggc gagataaata aataggccca tcttgcttct tttttctttc
    76321 ggatagataa acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc aaaaatggat
    76381 tattgatttt gcttgactaa gttttctact gctttttgtg catctgccaa tccttctttt
    76441 ggtgattttg ttcctaaaag aatctggtca atctgttcat tcactaatgt cgtgtaatcc
    76501 ggagcatcta aaggaactgg tgtgatcacc gtatttttaa agttttcgtc cgccatttga
    76561 tagatcattt ttcctttatc cgttaggttg tcattattca ctaaattctc attggctgtc
    76621 ttgctcgcca tgatatcaaa acttttttcg ccgaattttt cttgtacttc tggagaagaa
    76681 agatatttga tgaattcata agaggcctca ggatcttttg ctccgtatgg tacttccaag
    76741 acgaatccgc caccccacga ccaatggcct gaaccttctt ctttttctgg gagctgcatc
    76801 acgccaaaat caaaatcatc cggtgcattt tccatcaaac tagagtaata attgatattt
    76861 tgggcacgca tggctactaa tccggaaata aatggatcag atacaccaga cccaaattcg
    76921 gcttctaatt tattgatcgt atttttccca taataatctt gccagtcttg gatccactcc
    76981 aaggcttcca ctttattttt agtatcaatc ttgacttttt catctttgtc aaaccaacta
    77041 gtcccgttat ctgcgtttag cgcccataca tcagccccta aattccataa tggataaaaa
    77101 ccgatgcgtg taaattcttt tccttctttt ttatctagtt tacgtgctgt ttcttctaat
    77161 tcatcccaag ttgtcggtag atcttgttca ctgattccag cttctttaaa taacttttta
    77221 ttataaaaaa tgacttgtgt atctgtattg aatgggatcg cataaggatc accttcatat
    77281 tctactgtat cccaaagctg cggataaaaa tccttagaag tgtctttttc tacatatttg
    77341 gagagattag ttgcttgctg tgcttctgct ctttgggcaa cggaatttat gtcctgtaca
    77401 ataacatcgg gtgcatttcc agctgttatt gcagataaag atttagtcca gatgtcaccc
    77461 catggctgat acttataatt tacatggatc tcgttttgtg attgattgaa atcctcaatg
    77521 atttcttcga tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa atcaatattt
    77581 tttcttccat cacttgtttt ttcagaagaa tttgctgcac ttgaactggc atcgcttgca
    77641 ttacttgatc cgcatcctcc taataaaaac aatgctgaac ttgccagcaa ccctgtaaac
    77701 aataattttt tcatatttta cctccaaatt ttaatgtaac cgatgccggt tttaatataa
    77761 cgatatcatt ttgttctgaa aataacaatt tataaggtat gattctaaaa aaattattaa
    77821 tgataccaat actttatata attcgcgagc gctttctttg aattttttaa tctttcgtag
    77881 cagaatgaga atgtattaaa aatagattag gaggaatttt gatgtttaga caccagaaag
    77941 aattgcagtt tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa tatctgcagg
    78001 agctgatcgg cggccaatat ggcgagatga gtgtagctat gcagtacctg ttccaaggct
    78061 ggagctgccg aggagaagaa aaatataaag atatgctgat ggacattgca acagaagaaa
    78121 tcggccacgt ggaaatgttg gcgacaatgg ttgctcgttt attagagggt gcgcctgttg
    78181 aagatcaaga agcagcgatg caaaaagatc caacaatcgg tgccattatc tctggaatga
    78241 atccacagca cgcgatcgta gctggcttag gacctcgtcc tgctgacagc gtgggcaatc
    78301 catggagcgg tgcctatatc attgcgagtg gcaacttgtt agcagacttt agagctaatt
    78361 taaatgccga atcacaagga agacttcaag tagcacgtat ttacgaaatg atcgatgatc
    78421 gtggcgtaaa agatatgctg tctgttctcc ttgcaagaga tagctatcat caaaatctat
    78481 gggaatgtgc agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc ccaagtactt
    78541 tcccgcgtga caaagaacga aaagacattg cttatgactt ctataacttc tcagagggag
    78601 accaaagtag tgaaggttct tgggctagtg gcaaagcact ggatggcgaa ggcgaatatc
    78661 aatggttgaa agaacctaag atcgaaggcg ctgcaccaaa attagaacca gtaacaccaa
    78721 aaatgtatgg gacacctcca ttcaagaaat aaatatctac ccgtagaaac acccgttttt
    78781 tgttccacac gctttgtgag caaaactgac taaagtcatt aatcataacc acccttcaaa
    78841 agggtggttt tctctagagc tataagctca aatcaccggc cagcgcctaa agacgctggc
    78901 tttcatattt tatgttcaag ccatattgcc tttgccagtt ttgcccacct cttaaaaggt
    78961 ttatgtacta ctccttagcc cttaaaagga tcgtcttact ctcgtacact cagcttatct
    79021 aacgcactat catgtttttc ttgttcttga atgtactttt tgatggtcgc ttcattttgt
    79081 cctacttttg tcacatagta tccttccacc caaaagtgtc gatacccaaa tttatatttt
    79141 agatttgcac aatctcttga tgatctcctg aatattggct cgatattgat tataaattat
    79201 ttttcttcta tactttggtg tgaatacaat gtggtattta cacatctact ttgtacgggc
    79261 taaactattt ccctttattg ctccacccgc atagcaagtg gttttgtatt tcaatcgctc
    79321 ggcgcgctca attaggtcta aagacaataa atctaaaaac ccgctgcttc actgacagaa
    79381 acaacgggtt ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat ctattttatt
    79441 tttttgattt tttataaatg gaaaatgctt caagaagtcc aagaaataaa gccgtataaa
    79501 aacaagaacc tatgaaatct ttatttatta gatattcaac agaaatcccg ataacactag
    79561 ccaacaatat atttatcaga agtgtaaaat aaggatgctt tttatagaat agaactagtg
    79621 ttgttttcat agactctcct ttcttcatat ttccaattaa tcacaaattc acggcattac
    79681 tagaaaaatt aaaggggcgc agaagtggaa aaatactctt cgactttccc tacaaaccag
    79741 gccatcttgt gtttaataac tataccaaat ctaaggtacc tctaaatcta aattccattt
    79801 gtggcggtga cctccaattt ttaactcttc tatctcccat ccagtataca taaccttgat
    79861 acttcgacca cttatatatc gaacacacaa atacctatcc attgcaggag tactacctaa
    79921 cttagttgct acttgcatct aaataacagc tgaaaagata ggacttactc ttagtaaagt
    79981 atctgaacta ttaattttta aactatcatt tttatcctca actacatgca catctgttgc
    80041 aacttcactt tctgtctcat ccagataaat gattaaggaa tcgggaattg caatatcttc
    80101 agctgacgct ggaatagttg agctaaatta gtcatcatat ctttattata aaataaccaa
    80161 cagcaaaaaa tacaatgaaa aacaaacttg gaactaatgt atcattaata aatgttgaaa
    80221 aattcagcaa ctcattactg tatgatttta gcagcccaac caacatatac cctataaaag
    80281 ctaataagat ggttaggaat cctttagaat ttttactcat tcatagacac ttccttaaat
    80341 aacttttatt atagaaggta ttgtaaataa taatattgac aaaaagtgac tcaaaataaa
    80401 aaaaacctta cattatactg gaatgaact
//
LOCUS       DACDNO010000005        80328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDNO010000005 DACDNO010000000
VERSION     DACDNO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.005"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            173..445
                     /locus_tag="IWI22_000342"
     CDS             173..445
                     /locus_tag="IWI22_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167765.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            460..1383
                     /locus_tag="IWI22_000343"
     CDS             460..1383
                     /locus_tag="IWI22_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9167766.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(1523..2275)
                     /locus_tag="IWI22_000344"
     CDS             complement(1523..2275)
                     /locus_tag="IWI22_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9167767.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            2498..2860
                     /locus_tag="IWI22_000345"
     CDS             2498..2860
                     /locus_tag="IWI22_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9167768.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            3497..4969
                     /locus_tag="IWI22_000346"
     CDS             3497..4969
                     /locus_tag="IWI22_000346"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9167769.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            4981..5805
                     /gene="nadE"
                     /locus_tag="IWI22_000347"
     CDS             4981..5805
                     /gene="nadE"
                     /locus_tag="IWI22_000347"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9167770.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(5878..6768)
                     /locus_tag="IWI22_000348"
     CDS             complement(5878..6768)
                     /locus_tag="IWI22_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9167771.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            6966..8483
                     /locus_tag="IWI22_000349"
     CDS             6966..8483
                     /locus_tag="IWI22_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9167772.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8473..9744
                     /locus_tag="IWI22_000350"
     CDS             8473..9744
                     /locus_tag="IWI22_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9167773.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            9919..10815
                     /locus_tag="IWI22_000351"
     CDS             9919..10815
                     /locus_tag="IWI22_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9167774.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            10829..11692
                     /locus_tag="IWI22_000352"
     CDS             10829..11692
                     /locus_tag="IWI22_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9167775.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            11698..12261
                     /locus_tag="IWI22_000353"
     CDS             11698..12261
                     /locus_tag="IWI22_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9167776.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            12271..13794
                     /locus_tag="IWI22_000354"
     CDS             12271..13794
                     /locus_tag="IWI22_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9167777.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            13806..14765
                     /locus_tag="IWI22_000355"
     CDS             13806..14765
                     /locus_tag="IWI22_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9167778.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            14851..15930
                     /locus_tag="IWI22_000356"
     CDS             14851..15930
                     /locus_tag="IWI22_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9167779.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(16182..17582)
                     /locus_tag="IWI22_000357"
     CDS             complement(16182..17582)
                     /locus_tag="IWI22_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9167780.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            17800..19461
                     /locus_tag="IWI22_000358"
     CDS             17800..19461
                     /locus_tag="IWI22_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9167781.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            19418..20116
                     /locus_tag="IWI22_000359"
     CDS             19418..20116
                     /locus_tag="IWI22_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9167782.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            20132..21556
                     /gene="araA"
                     /locus_tag="IWI22_000360"
     CDS             20132..21556
                     /gene="araA"
                     /locus_tag="IWI22_000360"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9167783.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            21863..23022
                     /locus_tag="IWI22_000361"
                     /pseudo
     CDS             21863..23022
                     /locus_tag="IWI22_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            23140..24999
                     /locus_tag="IWI22_000362"
     CDS             23140..24999
                     /locus_tag="IWI22_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9167784.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            25109..25543
                     /locus_tag="IWI22_000363"
     CDS             25109..25543
                     /locus_tag="IWI22_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167785.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(25982..27004)
                     /locus_tag="IWI22_000364"
     CDS             complement(25982..27004)
                     /locus_tag="IWI22_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9167786.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            27132..27485
                     /locus_tag="IWI22_000365"
     CDS             27132..27485
                     /locus_tag="IWI22_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9167787.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            27497..28663
                     /locus_tag="IWI22_000366"
     CDS             27497..28663
                     /locus_tag="IWI22_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9167788.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(28755..29375)
                     /locus_tag="IWI22_000367"
     CDS             complement(28755..29375)
                     /locus_tag="IWI22_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9167789.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(29567..29863)
                     /locus_tag="IWI22_000368"
     CDS             complement(29567..29863)
                     /locus_tag="IWI22_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9167790.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            30067..30717
                     /locus_tag="IWI22_000369"
     CDS             30067..30717
                     /locus_tag="IWI22_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9167791.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            30733..32460
                     /locus_tag="IWI22_000370"
     CDS             30733..32460
                     /locus_tag="IWI22_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167792.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            32457..34349
                     /locus_tag="IWI22_000371"
     CDS             32457..34349
                     /locus_tag="IWI22_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167793.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            35053..35922
                     /locus_tag="IWI22_000372"
     CDS             35053..35922
                     /locus_tag="IWI22_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9167794.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            36131..37156
                     /locus_tag="IWI22_000373"
     CDS             36131..37156
                     /locus_tag="IWI22_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9167795.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            37195..38145
                     /gene="manA"
                     /locus_tag="IWI22_000374"
     CDS             37195..38145
                     /gene="manA"
                     /locus_tag="IWI22_000374"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9167796.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            38239..39942
                     /locus_tag="IWI22_000375"
     CDS             38239..39942
                     /locus_tag="IWI22_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9167797.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            39944..41977
                     /locus_tag="IWI22_000376"
     CDS             39944..41977
                     /locus_tag="IWI22_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9167798.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            41988..42428
                     /locus_tag="IWI22_000377"
     CDS             41988..42428
                     /locus_tag="IWI22_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9167799.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(42628..43755)
                     /locus_tag="IWI22_000378"
     CDS             complement(42628..43755)
                     /locus_tag="IWI22_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167800.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(43758..44435)
                     /locus_tag="IWI22_000379"
     CDS             complement(43758..44435)
                     /locus_tag="IWI22_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167801.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            44699..46465
                     /locus_tag="IWI22_000380"
     CDS             44699..46465
                     /locus_tag="IWI22_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9167802.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            46480..46917
                     /locus_tag="IWI22_000381"
     CDS             46480..46917
                     /locus_tag="IWI22_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9167803.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            46930..48081
                     /locus_tag="IWI22_000382"
     CDS             46930..48081
                     /locus_tag="IWI22_000382"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9167804.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(48143..48841)
                     /locus_tag="IWI22_000383"
     CDS             complement(48143..48841)
                     /locus_tag="IWI22_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9167805.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(48834..50186)
                     /locus_tag="IWI22_000384"
     CDS             complement(48834..50186)
                     /locus_tag="IWI22_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9167806.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(50409..51809)
                     /locus_tag="IWI22_000385"
     CDS             complement(50409..51809)
                     /locus_tag="IWI22_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9167807.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            51970..52707
                     /locus_tag="IWI22_000386"
     CDS             51970..52707
                     /locus_tag="IWI22_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9167808.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            52832..53311
                     /locus_tag="IWI22_000387"
     CDS             52832..53311
                     /locus_tag="IWI22_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9167809.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            53308..54075
                     /locus_tag="IWI22_000388"
     CDS             53308..54075
                     /locus_tag="IWI22_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167810.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEELVVYSRSQRRRHS"
     gene            54305..54874
                     /gene="lepB"
                     /locus_tag="IWI22_000389"
     CDS             54305..54874
                     /gene="lepB"
                     /locus_tag="IWI22_000389"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9167811.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(54978..55580)
                     /locus_tag="IWI22_000390"
     CDS             complement(54978..55580)
                     /locus_tag="IWI22_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9167812.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            55686..56155
                     /locus_tag="IWI22_000391"
                     /pseudo
     CDS             55686..56155
                     /locus_tag="IWI22_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            56261..56845
                     /locus_tag="IWI22_000392"
     CDS             56261..56845
                     /locus_tag="IWI22_000392"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9167813.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            56848..57921
                     /gene="prfA"
                     /locus_tag="IWI22_000393"
     CDS             56848..57921
                     /gene="prfA"
                     /locus_tag="IWI22_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9167814.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            57914..58753
                     /gene="prmC"
                     /locus_tag="IWI22_000394"
     CDS             57914..58753
                     /gene="prmC"
                     /locus_tag="IWI22_000394"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9167815.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            58746..58925
                     /locus_tag="IWI22_000395"
     CDS             58746..58925
                     /locus_tag="IWI22_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167816.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            58947..59972
                     /locus_tag="IWI22_000396"
     CDS             58947..59972
                     /locus_tag="IWI22_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9167817.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            60147..61391
                     /locus_tag="IWI22_000397"
     CDS             60147..61391
                     /locus_tag="IWI22_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9167818.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            61544..62491
                     /locus_tag="IWI22_000398"
     CDS             61544..62491
                     /locus_tag="IWI22_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9167819.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            62624..63253
                     /gene="upp"
                     /locus_tag="IWI22_000399"
     CDS             62624..63253
                     /gene="upp"
                     /locus_tag="IWI22_000399"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9167820.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(63392..63634)
                     /locus_tag="IWI22_000400"
     CDS             complement(63392..63634)
                     /locus_tag="IWI22_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167821.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            63755..63827
                     /locus_tag="IWI22_000401"
     tRNA            63755..63827
                     /locus_tag="IWI22_000401"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63788..63790,aa:Thr,seq:cgt)
     gene            complement(63946..64584)
                     /locus_tag="IWI22_000402"
     CDS             complement(63946..64584)
                     /locus_tag="IWI22_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9167822.1"
                     /translation="MNNILGKKIELKKGILGTIVVVFGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(64707..65339)
                     /locus_tag="IWI22_000403"
     CDS             complement(64707..65339)
                     /locus_tag="IWI22_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9167823.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            65690..65989
                     /locus_tag="IWI22_000404"
     CDS             65690..65989
                     /locus_tag="IWI22_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9167824.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            66484..68649
                     /gene="acm"
                     /locus_tag="IWI22_000405"
     CDS             66484..68649
                     /gene="acm"
                     /locus_tag="IWI22_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9167825.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(68921..69406)
                     /locus_tag="IWI22_000406"
     CDS             complement(68921..69406)
                     /locus_tag="IWI22_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9167826.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            69886..70527
                     /locus_tag="IWI22_000407"
     CDS             69886..70527
                     /locus_tag="IWI22_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9167827.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            70546..71181
                     /locus_tag="IWI22_000408"
     CDS             70546..71181
                     /locus_tag="IWI22_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9167828.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            71199..72014
                     /locus_tag="IWI22_000409"
     CDS             71199..72014
                     /locus_tag="IWI22_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9167829.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            72333..73769
                     /locus_tag="IWI22_000410"
     CDS             72333..73769
                     /locus_tag="IWI22_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9167830.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            73958..76405
                     /locus_tag="IWI22_000411"
     CDS             73958..76405
                     /locus_tag="IWI22_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9167831.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            76635..77702
                     /gene="rlmN"
                     /locus_tag="IWI22_000412"
     CDS             76635..77702
                     /gene="rlmN"
                     /locus_tag="IWI22_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9167832.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(77895..78998)
                     /locus_tag="IWI22_000413"
     CDS             complement(77895..78998)
                     /locus_tag="IWI22_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9167833.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            79551..80099
                     /gene="aac(6')"
                     /locus_tag="IWI22_000414"
     CDS             79551..80099
                     /gene="aac(6')"
                     /locus_tag="IWI22_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9167834.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        26854 a        13807 c        16529 g        23138 t
ORIGIN      
        1 cattcactaa taataaagat atatttacag tatcattata atgtattcaa tttaaaataa
       61 gagactttgg taataatgaa agaggaataa aacttttctt tggcaaaaca aactccttgc
      121 tatgagacgc gtttcataca ctatactcaa agtatcaaaa ggagggcttg caatgaatac
      181 ttatgtagag ataacagatg gcacaacgaa caattatttt tatgcgtttg tcgagttcaa
      241 gggatccgta ttaacgttgt acagttttca agggttgaat aggaatatag taaaagaaat
      301 accaatgaat gaaatcgaaa atttgacaaa agatatatat tggggcgggc agcggatcag
      361 tttttcgcat gaaggaaaaa tgtatcagtt ttttgaatgc ggtccagctg tggtagacta
      421 tttacaagaa aatctttttg tataggtggc gaagcagcag tggcaaacat tcgagatatc
      481 gcaaagctca caggttattc agtttctact atttcacgtg taatcaatgg acatccatat
      541 gtagatgagg aaaaaaggaa agaaatactt gcggtcatga aagaagtcga ttatatccct
      601 aatgcaaatg ccagacagtt aagttatgga aaaacgaaaa gcattggagt gattctgcct
      661 tataccaatc atccgtattt tgatcagttg atcagtggga tcattgaagc agcgtttgat
      721 tatgattaca aagtgacact tttaccgacg ggctatcata aagaaaaaga gcgaaggtat
      781 cttgaagaat ttgcagctaa agctttcgat ggattgatca ttacgtctcg tgccaataca
      841 ctagatgatt tattggcata tcaaaaatat ggacctctag ttttttgtga agaaatcaag
      901 ggaaaacagg caagctgtgt ctttattgat cgagaacaat caattacaga aggtcttact
      961 tatttgaagc agcaaggtat aaagcaattg ggcatcacgt tggggcgaag tggcaggcta
     1021 agttataact caaaaatcac tttgcagcta tgtcatcaac tttttccatc ttttgatgaa
     1081 tcacttgttt tttgggattg tatagatgca gagaaaggaa aacaagcagg cttctttttt
     1141 aaagagcttg gagttgatgg tgtctttaca aattcggata tggttgcagc aggcatcttg
     1201 caaagctatc tgcaagataa gacaccagtg gtgattggac gcgataattt gctgatcagc
     1261 gagcttcttg gtttcccaac catcgaccac catctggaag cttgcggaga aatggcattt
     1321 caattgtttc ttacagaaaa aaaggataaa gtgaaaatac cttatacgtt tattcaacga
     1381 tgaatatatt taaagtagga tgaaaaaact tgtgaaagaa tggagcagac accaaaagtt
     1441 agcataaaaa tctaacttgg gtgtctgctg ttttgttatt aggtcatttt acgtgtactt
     1501 ttttgttgga tcatgtaaaa gattacgcct ttttttggat aatttcattt aaagcttttg
     1561 ctgcaccatg accacttcgc tcgtcataat atgcagtgaa ccgttcatcg gcttcgtaca
     1621 tcattcctag tcctttatgc gcttttggct gatattcagg ccagctgtaa gagaaccact
     1681 tcttatgagc ttggaagata cggtctgcta agctttcatc ttctggttct tttaagtaaa
     1741 gagtgagatc aaataacatc tgcttttcaa cagtttgcat ttcttcatac gtttctttat
     1801 ccatattcat ccatttttca ttggatgctg taaccttttt ttttccgtat ttttgtcgaa
     1861 tctcttgtcc ataggattct tcattttctt tcaacttttg ttgtttaaac ccttcaaatt
     1921 tttctttgtc actcattttt cttcctcctt cgtacatatc aagtgtctgt tggacagttg
     1981 tcaagagggt atcgatttgt gcccgttttt ccagcaacat ttgctgatgc ttgattaaag
     2041 ctttctggtg gtcaaaagat ggattgtcta agacctcttg gatatcatcc aacttgaatg
     2101 ccattgttcg ataaaataag atgtgttgca gacgatcgac ttcttttttg ccataaatcc
     2161 gataacctga agaattgatc ctagctggtt ttagcagtcc aatctcatca taaaagcgca
     2221 aggttctcgg actgactcct gatatctctg acattttctt tattgtgtac tccattattt
     2281 ttcctcctga caaatgtaag cataatcctt tacgtaacga gatggtcaag aaaaaattgt
     2341 atttttttct cacgaaatta tgttatacta ttaagtgata aaaggtaaat ccttttgtct
     2401 ttaaaggttc aattgtaacc gattccttaa tgctaggaaa gatggtaaca taacttaaga
     2461 ttggatctaa atcgactata tagaaaagag gaatgagatg aagctaatcg aaacacctgt
     2521 gaatagtaat ttaaatatta agacatttta tcctaaagta gtggaattct tttttgggaa
     2581 tacggctatt aattattaca aattattttc gttagatcga acacaactgc tattggtaga
     2641 cacatatgat aaaaagcaag tcgtgatgat caatacgaag aaaaaaatca caaggcaaga
     2701 aatcgactat gcgattcatc acgttttaaa aatgacacga gaagatgtca aagtccatgt
     2761 aggagtgaaa caggagctag aacgtgccgg aatacaattc aaacgtccaa ataaagatat
     2821 tgtagtgata gaacaaaaaa atacgaagga ttctatttaa taacgactaa taatcatctt
     2881 tatggtaacc tgctccatat ctaatgtata caataaaaaa caaggcgaga ctgattgatg
     2941 tctcgccatt tttgtttcca ttctattgac agctgctttc ttactttgta tctttttaaa
     3001 aacgtgtgat attttttccg cgagtttttt caacgcaagg tatatcttgt cataattcgc
     3061 ctttccaaaa aaattcaata cataataagt tatatccgtt tctttgggga taagaagcaa
     3121 aaaaggaaag acgtattatt gcttcaaaaa cttttgccat tgattatgtt gtagagacat
     3181 ttggttatat ttgttcaatc atcggtatct taacggctac gattgtagta ggaaaatcta
     3241 cttattttat caagtaagaa aaatagaaaa cgctaatttc ttcgtgtctc ggatacattg
     3301 aataaaataa gtattctttc tcgtttaggt taaataccaa taagaaagga tagttttagc
     3361 agatggagtt catttttagc aagaaatatc cgtaaaacat gtgctttttc agaaaaaatg
     3421 gaagatttct tttcctatta attttttttg ttataatggg taggaaatga aaacttgcca
     3481 agaggagtaa tacaagatga ataatactta cccagacgac agtttgacgc tgcatacgga
     3541 tctatatcaa atcaatatga tgcagacata ctgggaatta ggtcgagctg atttacatgc
     3601 tgtttttgaa tgttactttc gagaaatgcc ttttaaccac ggatacgcgg tttttgcagg
     3661 attggaacgg ttagtcaatt atttagagaa tctgacgttc aatgattccg atattgctta
     3721 cttgcgtgga ttagaagtct atcctgaagg ttttttggag tatttacaaa attttgaatt
     3781 taaagcaaca gttcgttctg ctcgtgaagg agaactagtt tttgcaaatg aaccattaat
     3841 ccaagtggaa gggcctttag cgcactgcca attagtagaa acagcactgt tgaatatggt
     3901 caatttccaa acgttgatcg cgacaaaagc agctcgtatc aaatctgtca tcggagaaga
     3961 tccactattg gaatttggaa ctagacgggc acaagaactg gatgcagcag tctggggaac
     4021 tagagcggca tatatcggcg gagcagatgc aacaagcaat gtacgtgctg ggaagatatt
     4081 tgggatacca gctagcggga cccatgctca ttcgttagtc caatcttatg gaaatgatta
     4141 tgaagcattt atggcctacg ctaaaaccca taaagactgc gtgttcttag tcgacactta
     4201 tgatacgttg aaatcaggtg tgccaagtgc gattcgcgtg gcgaaagaat taggtgacaa
     4261 aatcaacttc caaggcgtac gcatcgacag tggagatatg gcctatatat ctaagcgtgt
     4321 tcgggaacaa ttagatgcag ccggatttac ggaagcaaaa atctatgctt cgaatgactt
     4381 ggatgaagca acgattttga atttaaagat gcaaaaagct aaaattgatg tatggggtgt
     4441 tgggacaaaa ttgatcacag cctatgatca gccagccctt ggtgcagtat tcaagctagt
     4501 atcgatcgaa gatgatgaag gaaaaatgat ggatacgatc aagttatcaa gcaatgcaga
     4561 aaaagtaact actccaggga aaaaacaagt gtggcgcatc actcgaaatt ttgatgggaa
     4621 atctgaagga gactacgtga cattatggga tgaagaccct cgagaagaag aagctatctt
     4681 catgttccat ccagtgcata cgtttatcaa taaaactgtt cgagacttta cagctcgccc
     4741 agttttacaa gatatcttta tcgaaggaaa aagagtttac gaattacctg cactaaacga
     4801 aatcaaagaa tatacaaaag aaaatctaga ttcactatgg gaagagtata aacgagactt
     4861 gaatccgcaa aaatacccag tcgatctgtc tactgaatgc tggaaccata aaatggctat
     4921 catggaacga atgaaaaaaa gcgtagcaga acttcataca gaagcatagg aggagaataa
     4981 atgagcctgc agacagaaat tatcaatgaa ttaggtgtca aaccaacaat tgatccaaaa
     5041 gaagaaatac gtaaaagtat tgactttatg aaagcatatt tgaaaaaata tccgtttctt
     5101 aaaacttttg tactaggtat cagcggcggc caagattcta ctttagcagg ccgtttggcg
     5161 caactaacga tggaagaaat gcgtgaagaa acaaaagatg aaaactatca atttatcgct
     5221 gttcgtcttc cttatggtga acaagcagat gaagaagatg caaaagctgc tcttgacttt
     5281 atccagcccg atgttagtct gcgagtaaat atcaaaccag ctgttgatgc acaagtacaa
     5341 gtattaagcg aagcaggtgt gggaatcagc gattttaaca aaggaaatat caaagcacgt
     5401 caacgtatga ttacgcaata tgccgtagct ggagaaagag caggagccgt attagggaca
     5461 gatcatgctg cagaaaacat tactggattc ttcacgaaat ttggtgatgg tggagccgac
     5521 attttgccat tattccgttt ggacaaacga caagggaaac aattgcttca agcattgaat
     5581 gcaccggaaa aattatacac aaaaattccg actgcagatc ttgaagatgg gaaaccaatg
     5641 attgcagatg aagtcgcact aggtgtcaca tataatgaaa ttgatgatta tctagaagga
     5701 aaagaagttc ccgctcaagc acaagaaaaa atcgaagcat ggtggaacaa gacacaacat
     5761 aaacgacatt tgccaatttc cgttttggat gatttctgga aataacaata aagcaaaaac
     5821 agccgtccct tcatagggat tggctgtttt tgttttataa aatagaaaag aaaaatatca
     5881 gctgtagtaa acttttaatt tgatatcctg atcgtaatta ccgaatcctt taccaaataa
     5941 agtacagccc cccacatgtt tggcatcgtc tttcacttcg attcgcaaat gccaagtgtc
     6001 ttgctcttta gggatgtcgt tgatcgtgat gtttgacaag ggttctccat tcatattcgt
     6061 caagtgtttg gtaattcgga tcgtcttcag caagccgtat tgattaagat tatctggata
     6121 ccaatcaggc gtatatttac cgcggatatc agcaaaatct ccagggctcg tccatgttcc
     6181 tagctccaca ccatttaggc tgaaagtgat atctgaaggc caattgtcat ttgaaaaagg
     6241 aaattcagaa gaaatttcca cagaaacttc caacatctcc aatgtatcct ctgtatttaa
     6301 aaaattaggt gcctgatact cgacaaatcc gctggtaaac cagagaatgc gggcatccat
     6361 ccttcgagga tccataaaat agcgtggttc atccactttc ccgataaaat catggatcgt
     6421 agctaaaccg catgttggtt caattgcata atttgtatag tgaccaatag gtatcgatgt
     6481 ttctttcgta tcaaaagcat tgaatatttt ttctggaaaa ctgatatcga ttttatcgac
     6541 acgaaggctc gaaatttttt gttgtcctac tttttctgtt ttgatgatgt ttgcttcctc
     6601 tagctttttc acgtgcatcg tcgtgatagc actgctcact ccaagttctt ttgccaaatc
     6661 tttgacattc atcttttttt tggaaagtag ctggatgatc ttgatccttg ttttactagc
     6721 taatgcttca tataccggta aggattcttc gtttatttgt agttgcatat atatcacctc
     6781 ttaaaaggtt tttcttatat attaacatat ttgttaataa aagttatatt aaattatttg
     6841 ttttaatcat tattaatatt ttggttaatt aaaatcgata aaaaataagt aagtttaaca
     6901 tttatattat tgacaacgct ttcaacgagg tgtattattc atgtataaat taaaggaggt
     6961 cagttatgga agccacaatg ttgattagta gtgtcaagcg tgtaggcaaa gttgatccgc
     7021 ggttgtacag ttcatttatt gaacatatgg gacgagcagt atatgaaggg atttatcagc
     7081 cagatcatcc atcagctaat aaaagtggat ttcgccaaga cgtcattcac ttggtaaaag
     7141 aattgaacat tcccatgatt agatatcctg gtggaaattt tgtgtcagga tttcgttggg
     7201 aagacagtat tgggcccata gcaaatagac ctagaaggtt ggatttagct tggcggacaa
     7261 ttgaaacgaa tgaagttggg attcatgaat tttataactg gtgtcagcat gtgggggccg
     7321 aaatcaatat ggctgtcaat ctgggtactc gtggagtaga tgcagctcgt aatttagtcg
     7381 aatattgtaa ttttcctaaa ggaacttatt ggagtgattt aaggcgttcg aatggtcaag
     7441 agagcccttt tggaattaag acatggtgtt tagggaatga aatggacggt ccttggcaga
     7501 ttggacataa aacagcggaa gaatatggac ggttagcaga ggaaacggct aaggcgatga
     7561 aactcgtaga tgattcaatt gaggtggttt tatgcggaag ttcgaatcgg caaatgccta
     7621 catttggtga ttgggagtta acagttcttg atcaagcgta cgatcaaata gattatttgt
     7681 ctctgcatca atattatggc aaccaaaaaa atgatctttc tcattatctt gcacgttcct
     7741 tagacatgga tcggttcatt tctgaagtga cagcgatgtg cgatacagtc aaggcgaaga
     7801 aacatagtaa gaagcagatc aacctgtcct ttgatgaatg gaatatttgg tatcattcag
     7861 cggaacaaga tgagcaagca gcgccttggc agattgctcc tcctttacta gaagataact
     7921 ataattttga agatgcatta ctattaggct gtctattaat cactttattg aaacatgcag
     7981 atagagtaaa gattgcttgt cttgctcaat tggtaaatgt tattgcacca atcaggacag
     8041 aaacaaacgg agaagcttgg agacagacga tctattatcc atttatgcaa gctgctgtgt
     8101 atggacaagg agaggtgtta agcccacaaa ttcgttcagc aaactatgcg acggaagatt
     8161 ttgaagaagt gccgtattta gaaagtatag ccgtaataaa tgacaaagaa cttgtcatat
     8221 ttgcagttaa ccgtggagaa gaagaaatga atctaactgt tcacttgtct gaattatcat
     8281 tagagggagt gattgatttt tctgaaatga gtggatttga tacaaaagca actaatcttc
     8341 ctaaaagtga acaagtaaaa ccaagtcatt caacaaatgt aaaagctgaa gatgggaaaa
     8401 tcaattgttt attgaaaccg ctatcttgga atgtggtcag atgtcgttta tcagatgaag
     8461 gaggaacaaa aaatgaaagg taaaaaatgg ttaattggct ctttgttggt agctgcaaca
     8521 ggtattttag gagcttgcgg tggcggaagt tcagcagaat ctgattctga taaagaagtg
     8581 actttgactt tttggaatgg gtttactgca tctgatggtg aaatattgca agaaatcgtt
     8641 aatgaattta ataaaacaaa tgataaaaac atcaaaattg aaatggatat catgacttgg
     8701 gccaatttga atgaaaaact tcctacagcg atttcttcta aaaaagcacc tgactttatt
     8761 gcattaaatt atccagattt tgcacagtat gtagaaaacg gagcggtaca accattagac
     8821 gatttctggg aatatgatgg agtagataaa tctgatttta aagaaacggc tattgaatta
     8881 ggtgaagtag aaggtaaaca gtattttgtc cccatgcagg tacaaggcat gtatatgtat
     8941 tggaataatg atttgtttaa acaagctgga ttagatactg aacaaccacc tactacttgg
     9001 gaagaattga cagaaatggc accaaaatta actgatagta gtaaaaatgt atcaggtttt
     9061 gtcttcaata aggatggaac tgcaccgtta tataactgga tgttagcaaa tggcggtaaa
     9121 ttagtgaacg atgattatac taagtctgaa tttgcgtctc ctgagaacct taaaacgtta
     9181 aaagcgatcc aaaaaatgat ccatgtagac aagactggtc cagaatcgat ttctggcgcc
     9241 gaaatggaca atttaatgaa tgctggtcaa ctagccattg aaatcaatgg gccatggtta
     9301 aataacggat tgaaagcaaa cgagattgat tataatgtga ctaccttacc acaagggagt
     9361 gattcagaca aaaaaacagc tattttagat ggcgtaggat atgccattcc ggcaagtaca
     9421 gataataata aaaaagaagc tgtctatgag tttttgaaat attggaatac aactgaaatt
     9481 ggtaaaaaat ggagtatcga aaatggtttt cctgcctact tggattcagt agcaaaagat
     9541 gaagaagtaa aaaatgatcc tattgtatct gaattatcga aacaaatgga ctatgcagag
     9601 ccatatttac caggttttac ccaaatctct gcgatcaata acgatatcat caatccactg
     9661 attgaaaaat tattagcagg agaagatcct gagacactca tgaacgatgc agatgatgcc
     9721 atcaatcaat tactgcaaaa atagtacaga actttataca ggatagactg ttggcaagat
     9781 acagatatct gaatgatcga cctagtattt gcagataggt gttattgcaa aaatcacagc
     9841 gattgttcgg atatcttatt cagattcgtt taaaccaaga ttgtatattt tatcaaaatg
     9901 aaagatgagg tggactatat gaacggattc accaaatatt ggaatcgtcc tgatcgtgct
     9961 gcgtatttgt ttttaactcc atctttttta gttttattat tatttactgt cataccgctt
    10021 ttaggaacat ttgctattag ttttacagat ttgaatattt tctttacatc aagaaatttt
    10081 gtaagttttg aaaatttcac acgtatattt agtgatgaac gagcagttaa ttcgttgatt
    10141 cattctttat atttcaccgt actcgaaacg ccaacacaaa ttatcgttgg tttattagca
    10201 gcagtagcat tatctaaaaa taccagattc aatcgttttt gtcgttcgac attttacatt
    10261 ccagttattt gttcattgac gtcaattgcg attatttttt ctatgttgct tgatccgaat
    10321 gtaggggcga ttccttactt gttttcacaa gtcggcttgc cgatccctca attttttcgc
    10381 gatccaaccc ttgctatgcc gacggtagcc atcatgactg tttggaagaa ttttggtgtt
    10441 actatgacga ttctcttgac tgctattcag ggaatctctc cctctttgta tgaatctgct
    10501 caaatggatg gtgcaacaaa tcgacagcag ttttttaaca ttacgctacc tcagatcgtt
    10561 ccctccttag gtttttgtat tttaacgaat ctgattggtt cgatgcaagt gtttgaccaa
    10621 gtatatgttg caaccgctgg tggcccacaa tttaagacag aaacagctgt tcaatatatt
    10681 tacagtcgag gatttacagc accttatgaa ttgggctatg catcagcact gtcttcggtt
    10741 ttgtttatca ttgttgcaac tatagctatc tccactaatg tgtacatgtc acggaaagaa
    10801 aaacaaatga aataggaggg aaacctgtat ggcaccagaa agctcgatta cagttactgt
    10861 gaatagtcga aaagcagtta gtggtaaaaa aatcattcga acgattagta tgctagttat
    10921 attgttggtt attgcgtttc cttttttgtg gctgattatt tcttctttta aacacgaaaa
    10981 agacattatc tcatttccgc ctagaatatt tgcagatagc tataccctag ataactatat
    11041 taaggtttgg acaacgatcc ctttattaga ttacatcaaa aatacagtga tttttgctgg
    11101 agggacagtg atcacttctg tattctttga ttctcttgcg gggtatgcat ttgctcgtat
    11161 gcgtttcaaa ggaaaatcag ttttgttcta tttcgttttg ctgacaatga tgatcccatt
    11221 ccaagtcttc atgattcctc tatttattga agtcaatttg cttggcatgc ttgatactta
    11281 tgcaggtttg atcattcctc gtatgacaac tgcatttggt atcttcatga tgcgttcctt
    11341 ttttattact ttaccagatt ctttggaaga ggcagcacgc attgatggat taagtgagtt
    11401 taatattttt ttgaaaatca tgttgccatt gagcaaacct actcttttat cattagggat
    11461 tttcacgctg atgaatagtt ggaatgactt gctttatcca ttgatattga caagtagttc
    11521 aaaaatgaga actttaccag cagggttagc tttatttaca gggcagaaca tttcttttta
    11581 tggacctgta atggctggaa cggttatttc aatgctgcca ttactagtgg tttatatttt
    11641 tgcacagaaa tattttgttc aaggaacagc tatgtctgga atgaaagagt gaggaagatg
    11701 aaaatagttg agactaaact ttatggacag ttgattgtgg tttctgatta catcttatta
    11761 ggtattctct gggtaatcgt gtgtttgcct gttattacta tcgtgcctgc tacatgtgct
    11821 gttttttatg taatggcgca atggaaaggt gagggatcag gaaatatcat ttattttttc
    11881 tttcaaggaa taaagcaaca ttttggtaag aacttgttcc ttagtctgtt aacgatcatt
    11941 atttattttc ttgttaatca attattagaa gaacagacaa tcgtactttc aattagtgga
    12001 tatgtagttt tacttattta ttccatgttt cttttatcct ggatatatgt gatttctgaa
    12061 aacaaagata tcacattttt acaaattttt gaacaaagtg catggaaagt attttttgat
    12121 tttttgagaa atattctttg ttgcgtattg ttattaggat ttgtactgct catatttctt
    12181 ttccctcctt ttatttttat ctttgctggt ggcgtatgga aacttgtatc tattatcttg
    12241 actataggaa atgaaaggta gggaaagctg ttggacgaag aagtgaaagt aaaaatcaga
    12301 gaggatttta cggtaggaga gatcagttct cggctgtttg gctcatttgt tgagcatatg
    12361 ggaagtgtcg tctatacagg gatttatgaa ccgggccatg aaacagctga tggtgacggg
    12421 tttagacaag atgtcctctc ttatgtaaag gaattagaat taggtgtcat tcgctatcct
    12481 ggaggaaact ttacatctgg ttatgattgg gtggatactg taggtaggaa agaacaacga
    12541 ccaaaaaaaa ttgatttagc atggcgaggt atagaaccaa attcttttgg actccacgaa
    12601 ttttttaaat ggatcagtca tgtacaagcg aaaccgatat tcacggtgaa tttaggaaca
    12661 aaaggaattg atgaagcaaa aaatttagta gaatattgta attttccaaa aggaaccttc
    12721 tggagtgatt taagaataaa aaatgggcaa aaaaagcctt ttggcgttaa attatggtgt
    12781 ttagggaatg agctagacgg tccatggcaa gtgggggcta agagtgctta tgaatatggt
    12841 aggttagcaa atgaagcctc aaaagtcatg aagttagtgg atgattcaat tgaaacaatc
    12901 ttagttggaa gctctacgcc acgtttacca tcgtaccctg aatgggatcg tatcgcatta
    12961 gaagaagctt atgagaatgt agattattta gctttgcata actatattga taagtatgat
    13021 gaagaagact tgactaaacc tcctaaaaat gagcgtgatg atacagcgac ataccttgca
    13081 aaagcttatc gattcgatcg tcaaattgaa gaaatcgttg ctacttgtga ttatgtaaaa
    13141 ggatatttga gaagtgagaa aacaatgtat ctagcgtttg atgaatggaa tgtacatgcc
    13201 caaccggaaa aaagacatag ggattttgaa gtcggtagtc ccatcgattg gtgttacttt
    13261 actatggaag atacattatt atttggttca ttagggctgg ctattttacg acacgcagat
    13321 cgcgtaaaga ttagttgtca atcattgtta gtaaacacaa tacctttaat actgacagat
    13381 gctaaaggac aagcatggag gaatccaact tattatgtga tgcaacactt atcgaaatat
    13441 gctaaaggtc aagtattgaa acaacaaatc atttgccctg tttatgattg tgaaaagata
    13501 aaaaatgttc cttatgtgga cagtgtgatc gtagataacg gcaatcagct gactattttc
    13561 ttgatcaatc gcacaaatga acaacaaaag ataacgattg aacatgattt caatttgcta
    13621 gatcaaggag agcactacac attaaccagt agcgatctgt tggacaaaaa tacacgtgaa
    13681 gaaccagaaa aaataaaacc tgaaataaac tatttttcaa tagaaaaaca tgacgaatta
    13741 aatgtaggga tagaaaaata ctcttggaat gtaattagaa taaagaaaat gtgaggaaat
    13801 ttgttatgat gaagacactt tataataatc ctatcgttcg gcaacgggca gatccttggg
    13861 tatataaaca tacagatggt tattattatt ttacaggatc ggttccaggc taccaagtga
    13921 ttgaattaag aagggcaaag tcactgaatg aattagaaca tgctgcaagc ctgattgtat
    13981 ggcaggctca tgaagaagga ccgatgagtg aattgatctg ggcacctgaa gtccattata
    14041 tcaatggaaa atggtatatt tactttgctg caagcaatca taaaactata cgtgatgaat
    14101 ctcatcatca ccgcatgttc gtacttgaaa atcagcatat agatccaatg aatacagatt
    14161 ggatagaaaa gggacaaata aaaacgaaaa gggaaacttt cagtttagac ggaacaactt
    14221 tctcttctca aggaaagcta tattatgtgt gggcccaaca agatcctgaa attactggga
    14281 attctaatct ttatctatcc gaaatggata atccttggac gttaaagggg aagcagatcc
    14341 tcctttctat acctgagtat gaatgggaaa aacggggttt ttcagtaaat gaaggtccag
    14401 cagtccttac tcgcaatggg aaaatattca ttacgtattc tggtagtgca acagatgaaa
    14461 actatgccat gggcctgctg tgggctgatg agcacaagaa tcttttatta aaagaatcat
    14521 ggcataaatt atcggagcca gtattcgttt cttctgaaaa aaataagcaa tttggtcctg
    14581 gtcacaactc gtttacggtt tcagaagacg ggtgctttga tattttgatc tatcatgctc
    14641 gaccagagaa gaaccaaaaa ggggatccgt tagacaatcc taatcggcac gcgaacgcgc
    14701 aatgctttac gtggaatgag caaggatttc cagaatttgg tgaacctgtt ccgtatgcct
    14761 tatgagcaca agaaaccttg atttttgctt gaataaacct tgcagactcg ttatgatata
    14821 tgtacgtaca aataaaaagg ggcaaggcaa atggcaaaat atcaagatat tgcagaagag
    14881 ataaaacaaa agatccttcg tgaggaatac gaagtaaata caaccattcc ctcagaattc
    14941 aagctgcaag aaatatatga cgtcagcaga catacgattc ggcaagcaat cgctgtgttg
    15001 gtaaatgagg gctatctgcg taaagaaaaa gggtcgggca catatgtaga tgacagctat
    15061 aagaaagaaa aaacaccatt gaacagaaca aaaacgatcg gtgtcatcac cacatatctc
    15121 tctgattata tttttccgtc gattatccga ggaatcgaaa atgaattacg agaatctggg
    15181 tattcattgc ttttagccag tacgaataac gatcacgaat tggagaaaga gtgtttgaag
    15241 aaaatgattg cttatggggt agatggtttg atcgttgagc ctacgaaaag taatcagtat
    15301 aacccaaact tagcacttta tgttgctttg agagaacaag gcattccgat ggtgatgatc
    15361 aatgctgctt atgaagagtt aaatgcctct tatatttgtt tagatgatgt gaaagtcggt
    15421 tttatggcag cagaatattt gataaaaaaa ggacatcagc gtctgttgtt catcacaaaa
    15481 atcgacgatc ttcaaggaaa atacaggatg aaaggtttta tcagcgcgtg tgaacagtat
    15541 aatgtgcgtt ttttgacgga agatatcatt actttcacca cggaaacacg agaaacaatc
    15601 gctgaaaaaa cagtacaacg gttgaaagaa gaacaagctt tagatggcat catttgctat
    15661 aacgatcaaa tagctagttt aatgctagat aagttgacag aaaatggcta tcatattcca
    15721 gatgacctat cgatcgtagg aacagacgat tcttctttaa gccatttagg gaataaaaaa
    15781 ttgacaacat tgacgcatcc aaaagaaaag ttgggacaag atgccgcacg gtggatcgtc
    15841 acaacgattg aaaaaggaca ggcacagcca aatattctct atgaacctgt actagttgaa
    15901 cgggaaacag tgaaagaaaa cgaaaaataa gagacgggtt tttaccgcct cttattgctc
    15961 ctacggttac tcgtcgcaat gtttgtgaga gcttttgtca tcgctcaagg ctgaacgttt
    16021 ctttcaccac ctctccacta aacggtgttc tcctaccaac tggtcacaag tcacaatatt
    16081 catcaaacat gagaagctgt tttttgaaat tgttccttgt gactggaaac taagcggttc
    16141 cttcttattt cttgaagctg accacttctg tcacaacctc tttaagaatg atggatagaa
    16201 atacgtttag gataagtttt tttagatgca ctttgttcga tttcctctaa agatttccca
    16261 cgagtttctg gtacgatctc ttgaataaat aaaaccccta aaaagcagat gatcccaaaa
    16321 atgccgaaaa cgatttcttg agacatagaa gcagtcatca caggaaaaag caagccaact
    16381 aggaaagaac caatccaatt aaaagaagag gctaggccag atgctcgccc gcggacagct
    16441 agtgggaata tttcgcctac gattacccat gtcagtggag cccatgtgca agaataaaat
    16501 gcgacataaa tacataaaaa caagagaatc aacaacgaat ttgtatgagc atccagtaca
    16561 gttcctaaaa cagctggtag gatgaaagaa agtcccatca cgctaccgcc tattttcaat
    16621 aaagttcgcc gattgaactt gtctgcaata accaaaaaga gcaaagaacc agcaactaaa
    16681 atgattcctt ggatgatcgg ccacatcaat gcatcgctcg ctgcatgccc agtcgctttt
    16741 tctacgatca gcggaatata gtaaaaaata gcattagctc cttgaaattg ctggaatact
    16801 gctacaccaa taccggcaat gactaaggaa cgatattttt ctaaaaacaa agtatgccaa
    16861 gaagttccgg ttgtcttttc ttgtttgaca ttcagctgga tttgttcgaa ttcctgttca
    16921 gcttcttttg gtgtccgaat atagttcaat actctttttg cctcttccaa gcggccagat
    16981 tgcataagaa aacgcggtga ctctggtaga gctaaaacac cgaaaaataa aatcagcgcg
    17041 gggacagcag cgagccctaa catcacgcgc caagccatcg tttcaggcaa cccttttagc
    17101 aaataatctg caacatagga aaggagcatg ccagaagcaa tcatcacttg attgatccct
    17161 gacaatcttc cccttagtct agcaggagcc atttcagaca tataagcagg aactagggca
    17221 gatgcagcgc caacagctaa acctagaaga atccttgctg caatcagaaa ataagaacca
    17281 ttgtgagggg cagatgcaga aaggacagaa ccagccataa aaataagtgc agaaagaaga
    17341 atcatttttc gtcgtccaag cttatcagaa agaagaccag ataaagagcc gccaaggatc
    17401 gcacctaaca tgaccgaaga cgtgatccat cctatcaaag aagctttacc tgccaaaccc
    17461 caatcatgct gtaaaaaagg taaggcgccg gtcataacac caatatcata tccaaataga
    17521 atgccgccaa atgcgccaaa aaaataaata aacgatgagg aaactttttt ttcttttttc
    17581 ataagtattc tctccttcta gaatttcgag aaggcggttg atcagatttt gtaagcaatt
    17641 tcattttata acttatgtac gtacaaaaca agtgtttttt attgtattct tgtaaataac
    17701 tgcttgacat gtacgtacaa aagtatacaa taatcattgt aagcgctact aaaaagacgg
    17761 tttgatcagg tttgacgaga aggagggagt taaatcatta tgaaggaagt aaatgcgcaa
    17821 agtatgttag atgtcataga tggtcgtaca tctttaggga ttgagtttgg atcaactagg
    17881 atcaaagctg tattgattga ttcatctttt actcctattg ccgctggtag ttatgagtgg
    17941 gaaaacaaac tagaaaatgg tatctggacc tattcattag aagatatttg gaaaggactg
    18001 cgcgtcagtt accgaaaaat ggcagcagaa gtattcgaaa tctatggcga gaatcttacg
    18061 acaatcgggt cgattgggtt cagtgcaatg atgcacggtt atctagcatt tgatagcaag
    18121 gaacagttac tagtaccatt tcgtacttgg cgaaatacaa tcactgaaga agcagaacga
    18181 atattaacgg aaaaatttca atacaatatt ccgcagagat ggagtatcgc tcatttgtat
    18241 caatctattt tgaataaaga agaacatgtg aaagaaatta attttttgac aacgcttgca
    18301 ggttatgtcc attggcagct tactggtgag aaagtactag gcgtaggaga tgcatcaggc
    18361 atgtttccta tcgatccttc aacgaaaacc taccatcaag aaatgaaaaa tatttttggt
    18421 cagttaatcg aaaaagcgca cgtagcgatt gacttggatc acattttgcc taaagttcta
    18481 gtagcaggag aagaagctgg caagttaagc gaatcagggg cttatctgat cgaccctacg
    18541 ggaaatttga gaccaggaat cccggtttgt ccgccagaag gggatgcagg aaccggtatg
    18601 gtcgctacga acagtgtagc caaagggaca gggaacattt cggctggtac ttccgccttc
    18661 gctatgatcg tattagaaaa agaactagaa aatgtccatc cggaaatcga catggtcact
    18721 acaccttcag gagaactagt aggcatggtt catacgaata actgctcttc ggatatcaat
    18781 gcttgggtca gtatttttga agaatttacc aattctttgg gtatggagat aagcagagat
    18841 aaattattca cggtattatt caacaaagcg ctagaaggcg atacggattg cgggggatta
    18901 ctttcttatg gttatttttc cggtgaaaac attacaggag taaatgaagg aaggccatta
    18961 tttgtacgta caccaggtag ccgttttgat ttagcgaatt tcatgcggat ccatttagcc
    19021 agtgcttttg gtgcaatgag aatcggaatg gatatattga aatctgagaa tgtccaaatt
    19081 gatcgtttag tggggcatgg ggggattttt aaaacaccag aagttggaca gcggattcta
    19141 gcatctgcta tggaggcacc agttacagtg atggacacag ccggagaagg tggagcgtgg
    19201 ggaatcgcgt tattagcagc ctatatgatg gatcgacatg ggaagtcttt agaaaacttc
    19261 ttggcagaag acgtgtttgg acaagaccaa ggaatcacca tcacaccttc agcagaagaa
    19321 atagcaggct atcagatatt tatgagacgt taccgtgagg ggatcgacat tgaaaaaaca
    19381 gcgattcatc aattgcaaga aaaaggagtg atagaaaatg ctggaacaac tgaaagaaga
    19441 agtataccaa gcaaatcttg acttgcctaa acacggactc gtcaaatata catggggaaa
    19501 tgtgagcgct tttgatcctg aaacacgata tttcgttatc aaacctagcg gagtcagtta
    19561 tgaagagatg acagcagacg acatggtcgt tgtggacttg gacaatcata tcattgaagg
    19621 taaactcaat ccctcttcgg atacgcctac tcatgctgtt ttatatcgtt catttcctca
    19681 aatcggagga atcgtccata ctcactcgac atgggcgacg atctgggcac aagcaggatt
    19741 ggatgttcct gcgatgggga cgacgcatgc agatactttt tatggatcgg taccttgtgc
    19801 tcgatttttg acacaacagg aaatcgacag cgggtacgaa tatgaaacag gaaaagttat
    19861 catagaaaca ttcaaagaac gcaaaattga tcccttagca attccagggg tacttttaca
    19921 cggacacggt ccatttactt gggggaaaga tgcgcaaagt gcggtaatga acgcagtcgt
    19981 actagatgaa gtatgcaaga tgaatttatt taccagacag ctcaattcat tttcagaaga
    20041 gctgcctcaa cggattttag ataaacatta tttacgaaaa cacggaaaaa atgcgtatta
    20101 cggacaaaac aaataaaagg agtggctaat tatgttgaat attggagaaa aagaattttg
    20161 gtttgttgta ggatcacagc atttatatgg ggaagaagca ctgcgagaag taaagaaaca
    20221 tgcacaagaa atggtcgatg aactgaatga aaatggccaa ttgccttatc caatccgcct
    20281 tcaagaatta gcagttactg ctgatacgat cacaaaaatc atgaaagagg tcaattatcg
    20341 agaagaagta gcaggtgtga tcacttggat gcatactttc tcaccagcaa aaatgtggat
    20401 ccgcgggaca aagcttctac aaaaaccatt gcttcattta gctacgcaat ataacgaaag
    20461 cattccttgg aaaacgatcg atatggattt catgaattta aatcaatccg ctcatggtga
    20521 ccgcgaatat gggttcatca atgctcgatt aaataaacaa aataaaatcg tcgttggcta
    20581 ctggaaacga cctgagatcc aaaaagagat tgctgattgg atggatgttg cagtagccta
    20641 taatgaaagt tttggaataa aagtggctcg ttttggtgac aacatgagaa acgtgggtgt
    20701 cacagaagga gacaaagtgg aagcacagat tcaatttggt tggactgttg attattttgg
    20761 tatcggagat cttgtacaag taatcgatcg cgtatcagac gaagaagtcg atcaattatt
    20821 tgaagagtac aaggaattgt ataccttcga ctatggtgat tatgaagaaa agacatggga
    20881 ggaacatgta aaagtacaag ctcagcagga aatcggtatt cgccgtttct tagaagaagg
    20941 aggctataac gcttttacga ctaattttga agacctgtat ggaatgaaac aactacctgg
    21001 actagctgta caacgtttga tggcagaagg atacggtttt gctggggaag gcgactggaa
    21061 aactgcagcg attgatcggt tgctgaaaat catggctcga gggaaagata caggattcat
    21121 ggaagattat acttacgagc tagctagtgg ccaagaagca atcttggaat cccatatgat
    21181 ggaagtagat ccgacgcttg ctgcgacgaa accaagaatc gttgtttctc ctttatcaat
    21241 gggagataga gaagatccgg cgcgtcttgt atttgatggg aaagcaggag aaggggtagt
    21301 cgtatcgatg gcagatttcg gtacgcatta taaactgctg atcaatgaag tagaagcctt
    21361 tgaaccaacg actgaagcac ctaatttacc tgttgcacgt gtgttatgga aaactaaacc
    21421 gaatttccat gaaggtgtcc attcatggat tcaggcaggt ggcggtcatc ataccgttgt
    21481 ctcgctaaat ctaacaacag atcaaattga aacgtgggca aaattagttg agttggaaac
    21541 agtggttatt agataaaaag aggttgtgaa aaagccactt agttttaaga aataagaaga
    21601 gattgacgaa atttgctttt caactttttt gaaatttagt cttattatca atgtcagcta
    21661 acggaacacc atttatcaag atatctcacc ctcaatattt tgctctcgaa aataaggctt
    21721 atggcagtaa cttgtcatag ccttattttt atcactcagc ctactctgct ctaactgctc
    21781 tttttgcttc taggaagtca aatggtaaac agttgtctaa ttgcagaaat ttcgagatac
    21841 ttgttagtaa ggagggggtt ttatgtatca cgttcatttg attgatgcta ttccagaaca
    21901 agagatagaa aagttacaag gggaatattt caaaataata gatgatttgc cagccgaagg
    21961 aatagttatt cgtagtacga aagtgaaaga tgaatggctt acaccagatt tgctagcaat
    22021 ttcaagagct ggtgttgggg tgaacacgat caacgttgaa aaagcttctg aaaacgggac
    22081 gatcgtcatg aatacaccag gtgtaaatgc taatgcagta aaagagcttg ttttgtgctg
    22141 tttgctctta tcttcccgtc cgataatcga agcaagtcgt atggtacaaa cactaacagg
    22201 tcctaatatt ttagaacagg cagaaaacaa aagaagtgct tatgtgggac gagaattgca
    22261 aggcaaaacc atcggtcttc ttggtcttgg agcgataggc acaaaagtag cgctaagttg
    22321 ctatagctta gggatggatg tcttgggtta ttctatccgc gatgcccagt tggattatgt
    22381 tcggcaagca gatttagaaa cagttctttc tacctcggat tacatcgtag tcatgctgcc
    22441 gttgacagaa gatacaaaag gattgatcga tcaggcgaat atagaaaaaa tgaaaaaaga
    22501 tgctgtactt atcaatgtag gaagaagtga aattgttgat aaatatgctg ttatgcaagc
    22561 attggaaaaa caacatctgg ctaaatactt gactgatttt ccagaagaag agttcttaga
    22621 aaatgaccgt atacttatgt tgcctcatct aggggatcga ctcaggaagc tttggcagac
    22681 agcggacgtt tggcagttga agccttaaaa gattatcttt tgttcggtac ggttagagaa
    22741 gcggtcaatt atccatccgc acgaatgttt ttccaagcgc cgtttcgatt cactatattt
    22801 tatcaaaaga aaaccaatat attagcagag atattttctc tgctgaatga gaacgagcta
    22861 gcgatcgcag atatatcgcg taaccataag aatgagcatg tctacatatt gattgacata
    22921 gacagtactg attttgaaca gctcaatcaa gtgaaacaac agctagaaaa aatttctgga
    22981 gtacgaaagg tccgcttatt aaagaaacca gaaaggccat aaagaaagag aatatttgga
    23041 tttcattaat tcttctttcc gttctttact ttcgaatttc gtacaactaa aataagagaa
    23101 gcaacattct tttattgata gaaatgaagg agcaagcatt tgaaaagata tataagcaga
    23161 ctttcgacca aacagaaaaa gattttaaaa ggaacagcag caatcatcag tatctttttt
    23221 cttgttgttt ttagtaattt atttttgcag tggtgtcaaa atgatctatc cgtagacctt
    23281 gcgttgaaat ttgctttttc ttggcatacg gaaaaattct tcttagcctg tctcgtcttg
    23341 ctgattattc ttatttttct gattgcacta gcaggatctg tccctttagg ttcccttacg
    23401 tatgtcgtag ccatcggtgt attaggtttt gcaaattata tgaaaatgag ttatcggcaa
    23461 gaaccgattt atccagacga tttgaagatg atcactgaaa tcggattgct gaaagatatg
    23521 accggcacta tgctgtttac agtgatctta gctgccgcag gaaccgtgat aggattgttt
    23581 tgctggtaca tgtttcgaag cctgaaaaaa ggaaggcgat tccaattgat ccgattaacg
    23641 acactgcttg tggcaattgg actgctaggg tatatcagta atttcaacaa tcccgataat
    23701 ttgttgcgaa aagcatataa taaaacggca ctatggatac cttacagcca gaaaatgaac
    23761 tactataaca ctggttttat cggtgggttt ttgtacaatc tgaaggtaga accaatggat
    23821 gaacctgaag gatattcgaa agaaaagatc aaagaaatca ctgaaaaata ccagaagcta
    23881 gccgatgaaa aaaataaaac tgtagaggaa gagtcgccga acatcgtgtt cgtgatgagt
    23941 gaaagtttct cagatcctag tcgattaaac ggcgtagaag tatctggtga gccattggca
    24001 gattattatg aagtagctga tcaaacatat agtggcaata tgctttctca aaactatggc
    24061 ggtggaacag caaatatcga atttgaagca ttgactggtt tttctatggc gttatttaat
    24121 gcgcaactca ccacccctta cacgatgctt gtaccaaaaa tggatcagct tccttctatc
    24181 gtttcagcgt taaacgcaca atcataccag acgacagcga tccatccata caatacttcg
    24241 atgtataaaa gagaggatgt ttatcagaca ttaggatttg accagttcat cagcgagcgt
    24301 acgatgacct atacggatac gatagagaat aatccatata tttctgatga gtcagcttac
    24361 aaagaagtaa tgacacttct caaggaagaa aaaacacctc agttcattca tttagtcacg
    24421 atgcagacac atatgcccta cgatggaaag tatgataatc tagactatcc tgcaaaaact
    24481 tcagatggaa gtgggacact tgacttagag aattatctgc aagatatttc ctatagcagc
    24541 acggcactga agcagttcac ggaagaattg aagaatcttt ctagaaggac tttagttgtt
    24601 ttttgggggg atcatcttcc agggatctat tcagatacga tccaagcaaa aaatgacaaa
    24661 caaacattgc atgaaacaca atttcttatg ttcgacagta aaggagaatt agaaaagcaa
    24721 acgactcacg atgcaatcac cagtccgttt tattttgcag cgaatctaat ggaacagacg
    24781 aatcagacaa caaatggctt ttatcagctg cttttatcat tagaacagga acttccggcg
    24841 tttgaacgag aactgtacta tcaaaacgga cagtggtata aagaagcgca attcaatcgc
    24901 tcgcaacaag agatttacga cgaatatcaa ttgatccaat atgacattgt cgcagggaaa
    24961 caatatagtt tagcagatgg tttctttgag cacgaataag aatggcgagc tgtatagaaa
    25021 ccattaaatt ttttcaagta cttgtcattt gaggataaaa acaagtagaa taaataataa
    25081 caacaatgga gaaagtagga aaacgaacat gttaccgaat attggctatt atgtgcagtc
    25141 catcgatcaa atcgtcaagg aaacagaagc aatcggagaa ggaatgcatc cgaaatatga
    25201 agtgatcaga aacgcaattg atgaaggaaa aacaagtgaa ctggacaaat cgactcttga
    25261 agagatcatc ctgttatttg aagaaggaac aaaaaaatat cgggcaatgc ttatgaaagt
    25321 cagtactttg cgtccaccag caaaagcaat gggcattcat aaaaattttg aaaaagctta
    25381 tctttcatat gtagcaggct gtgaagaaat gatccaaagt atcgatcttg aatcaggggt
    25441 agatgaagat ctattcaata catctgaagc aaaacaagac caagcgacag accaattgac
    25501 agctgccatc caaaaaatga ctaatttgtt gttgaaaaaa taaaagaaat ctttgataac
    25561 aataaaaact agagttatct aatataaatg taatattacc tttatgatag agtgatcaaa
    25621 atcctttatg ataatgacat acctataacc aacaacaacc ttgtacttac tcaaaatcga
    25681 gtaaatactc cttttccgtc gagtctaacc atctcggcgg ttttttattt gtagaaaaga
    25741 acatcaggat agacggaaaa gtcttgtcct gatgttcttt tcagatagta tggaaagctg
    25801 attagttttc tacattctgt acatattgtt caaagtagtt ctgtaaatcg gctttgagtt
    25861 tctgatattc ctcttcgcta tactctttat cttcgatttt cccatcaaag aaaggaatat
    25921 ttagttcttc ccgtagtctg tctaatacgt cttgctgatt catttcattc tccttttctt
    25981 actaacgatc aaaataaaca cagacacatt ctggtacata gacatctttt ttcagtaatt
    26041 ccagtaatcc tgtttcagga tgacggcgat ataatgtcag attatcggtg ttctgattgg
    26101 cacacacgac aaaattattg cttggatcta gtgcgaaatc gcggggaaaa tccccttctg
    26161 ttgagatgat ttgcttgttt tgaatgtggg aaccatcttc aaaaactgcg aatacagcaa
    26221 tcgagttatg tcctcggtta gaagcataaa ggaacttgcc atcatttgaa atgcggatag
    26281 ctgcccctcc attttcaccg tcgaattctt ctggcaaggt cgatattttc tgtaattctt
    26341 caaaagcacc tgtttcttta ttataagaaa gcacgcggac tgtgctgtct aattcaccaa
    26401 ataaataagc gaattgtcca tttggatgaa agacaagatg gcgagggcct gttcctggct
    26461 ctgcagtgaa ggtagttaca agattcaatt tgccgttttc ggacacatca taagtataca
    26521 cacgatctgt accaagatca catacgacta atcgttggtc aggagtcaga tccgtatagt
    26581 ggacatgcgc atgatcttgg tttttatgag gacctgtcgc ttcttgatgg aatattgaat
    26641 ctgctgcttt cagttcgcca tctcctaata tatggtagac atttacttca cctttatgat
    26701 agttcgcccc gtatagtaat tgacgtgctt catcaaccgc tacataacac aaaggagcgc
    26761 cagactctgt taccgcattc aaaaaatgaa aatccttatc gtacgcaccg gcaccaccta
    26821 atccatccac actagtaact gtgtaaagat tgccagcttt actttttgct acataggtag
    26881 ggctattttc ttcaacagct aatgaaaggt tggctaattc ttctttttct gtatcaaggt
    26941 cgatcgtata gattccttta ctttcgcgac gcgtataggt tcctaaaatt atcttttcga
    27001 tcatatgagc tgattcctcc taattgtaat gaaatagttt tcgtttttat tctatcacat
    27061 aattattcca cacggctaaa gaatgagaat gtgttatact gtttttgaaa gcaattaaag
    27121 gagagagtgc aatgattcag gcaaagatta cagaaattgg gacagaagca ataaacaagg
    27181 aagaacccat gctgattttg ttcgataaaa cagcaacagc tgccttacga aaatattcgg
    27241 tgattcaaga agtatcaaaa gacgaacctt tttctttgaa agcaggcgga acaattgctt
    27301 ttgatgatca ggtatatacg atcgattatg tcggtccgat ggccaatggg aatttaaaga
    27361 cagtagctca cgtatcattg atttttgatg cttgtccaga agaaggacaa attgcaaatg
    27421 gaatctatct tcatccgcat agactgcctg ctattggttt gggaagccaa attttttata
    27481 actaacagga ggaattatgg aaggaaaaaa taagaagaac agaccggtat cgtggttttg
    27541 gcgctggttt ttaaataatc aagtagtcac agccttactt gttgtattgt taatattgct
    27601 gatcgtcttt ttatttacaa aagtttcata tttgtttgca ccaatctggc aatttttagc
    27661 aatcgtaggt ctgcctatca ttttagcagg aatcttgtat tacttgatga atccggtagt
    27721 tgattacttt gaaaagcgaa aagtaccaag gctttacagt atcattgggc tgtttgtttt
    27781 ggttgttgca ctaatcgcat gggggagcgt agtgatcatc cctaagattc aggaacaaac
    27841 agtgagcttc atcagtaact tccctcaata tgtagatacg attgataata aattaaccga
    27901 gattttgcgg gacccgctgt tcaaccaatt tcgcgaacag ttggaaacag caggtgataa
    27961 gttcatgaat tcggcaggcg atatgatcca agatatatct aagagtacag ttcagagtct
    28021 tggaagtttt gtcggcgcgg tagcgactat tttagtggct ctgctgacga tgccgtttat
    28081 cttattttat ctattgaaag acggtaaaca attagcacct tattttgtca aatttttacc
    28141 gacacgcatg caaaaaccga cgttgaatgt gctgagtgaa atgaatagtc aagtttcttc
    28201 ttatataaga ggacaactaa ctgtggcttt tgctgtggca atcatgttta tgattggttt
    28261 tgcggtcatt ggtttagagt atgctgtcac gttggggatt attgcgggtt ttttaaacct
    28321 gatcccttac ttaggatcat ttttagcgat gattccggct atctttttag ccattgtagc
    28381 aggtccattc atgatcatca aagtactgat cgtttttgca gtagagcaga caatagaagg
    28441 acgcttgatc tctccattag tactagggaa tcagttagca atccatccag taacgatttt
    28501 attagttctg ctgacttctg ggaaattgtt cggaatcatc ggtgtgatct tggggattcc
    28561 tgtttatgca gcagctaaag tagtgattac tcatgttttt gaatggtaca caacaatctc
    28621 ttctctttat gaagaaaatc cgttgaaaaa gcagaaagaa taacaaaaag agacagatgg
    28681 gaaaatatcc aaggcacaaa tgctgctttg aagatttttt cctctgtctc ttttgtatgc
    28741 atttatctca atttttaaaa tgatttacct aaagcagcag ctttatctaa tgcattttgc
    28801 atcaactcat ctgcacggtc agggtcataa tcgattcctt cgatgaatag ctgatctact
    28861 tgatcgatgc ctacaaagtt taaaatacct tttacatatt gagaggcaaa gtcttgtccg
    28921 ttgtatacac cgccattaga ttggatgtgc agtgcttttt tacctgtcac taatccttct
    28981 ggcccgtttt ctgtatagcg gaacgttttt ccagctacat tgacggtatc gaaccaagct
    29041 tttaattttg ttgggatatt tagattccat aaggcgtttg cgataacgat tttatcagca
    29101 gcaaggaact gatcagtcaa ttcattgaaa cgagcaattt tttgttgttg actttctgat
    29161 aaggaagtga attctgcacc agcacgtaat gcttcccaac ctgataataa ttcttcatcg
    29221 atttccggaa tatcatcgcg ataaagttcc aaaacagtca cttcatcatc tggatgattt
    29281 tctttataac ttgttaaaaa agacgtcaat gccttaactg tgcgcgattc ttctgcagtt
    29341 aggggatgac ctttgactac taaaacagat gccatatgct taccttctcc ttttattgct
    29401 atgattgtta taactatgac taacaaaatc agtttactcg aaaaagaaaa ggctatcaag
    29461 tcatattgat aaggttgaag aataaataac taaaaaaaga cagggtataa aagaggctgt
    29521 gacacgattt tttgtcacag cctcttttga atctaacaag caatattcaa gcaacactcg
    29581 actgaagagc ttccatccca ccaatgatat aaacagcacg aaaaccatta gcagtaagtt
    29641 gatcggctaa tgcttctgat cgcctgcctg tatagcttag aatataataa agtttctttg
    29701 gatctagatt ttttaattgt cttggcaaga tagcagcggg gatagaaagc gagtggctta
    29761 agtggttttt tgtgtaatct aaggtatttc ttgtatctaa aacgatgctg tcagcatcga
    29821 gaggcaattt attgaagtct tggacactaa ttgattgggc catttatctc atcctttgtt
    29881 tagtatgtgg ctattttaat gggcaactct cattaattca aatggaagga ttcctcttat
    29941 cactttttta atgtatcgga tgataatgaa aaaaactatg caaatgtctt gcaaaacagg
    30001 taggaggtgt tataatgaca tagcgtcata tgacaatgtg tcatcttgaa agaaaggaga
    30061 atccatatgc cgacacagac atttttccat ttgcctaaag aaaaacaaaa aagactaatc
    30121 gaggctgcga gaatcgagtt ttctagagta cccttaaaag aagcttcgat tgctaatatc
    30181 gtcaaattag cggatatccc tagaggcagt ttctatcagt attttgaaga taaagaggat
    30241 ctttattttt attactttga gacaatgaga cgtgacagct ctaaagatat gttacaacta
    30301 atgaaagaat ccgatggtga tctattcaaa ggattcgaat cctatttttc taaaatgatt
    30361 tatgagattt tggaaggcga gaatgcttct ttttatcgta acttgtttat gaacatggat
    30421 taccgtgcct caagaaaagt cgctcctcat acgagtcgcc aaaaaacgcc tcacgatcca
    30481 cataaagaac acaaacaaga tgcaaaagag ttgcttgaat tgatcgatcg gacgaagctg
    30541 aaagtaaaaa atgatcatga attggaatta ctgatccaat tagtgatgaa tactgtattt
    30601 acaacaattg cgcattcgta tcgcttattt tcaacatctg aagattactc agtagagcaa
    30661 gcaatcagtg aattcaattt gaaactttct tggctgaaaa acggagtata taaatagaaa
    30721 gaagggaact agatgctaaa aatcgtaaaa cggatctcct tgacttcagc aattgcagct
    30781 gcagttttca tggtgatcca agtactggca gatttatatt tgccgacact tacctccaat
    30841 attattgata aaggggtagc gcaaggagat gtcgattata tttggcatac aggttttgtg
    30901 atgatcggct tttctttgat cagtatctta gctgctatcg caaatacatt ttttgctact
    30961 cgtgaatcac agaaactggg taagcaattg cgtacggatg tctataaaaa atcagagagt
    31021 ctgacaaaag atgcgtttga taaatatgga acggcctctt tgatcacaag aacgaccaat
    31081 gatgtgactc aaatccaaat ggttacacaa atgtttttac gtatgatgat caatgcacca
    31141 atcacgttga ttggcgcgag catacttgct tatcaaaaag atcatcagtt gacaaaaatc
    31201 ttcttagttg tcatcccagt aatgattatt ttgatcggcg gaatcatgta ctttgctgtt
    31261 ccattattca aaagtatgca aaagaaaact gaccgtttaa acctggtatt tcgagaagga
    31321 ttgacagggg tcagagtgat tcgagcattc gataagacac gctttgaaga aaatcgtttt
    31381 gatttagcga ataaagatta tacaaatacg gcaatcaaag ttaatacgat cgtcgcattg
    31441 atgatgccaa tgatgacatt gatcatgagc ggtacaaacg tagcaatcac ttggtttggt
    31501 ggacactaca tagcagatat gactttggaa gtcggtaatc tgattgcatt tatgacttat
    31561 gcgatgcaga tcttgatcag ctttatgatg ctttccgcta tttttatcat ggtgcctcgt
    31621 gcacaagctt cagctgaccg tattaatgaa gtgttggatg aaaaaatcgg tatccatgac
    31681 cctgaaaatc ctaaaacagt ttcttttgct ggaaaaaacg caacattggc cttcaatcat
    31741 gtcaattatc gatatcatgg agcagaaaaa cttgcattgg aagatattga tttccaagcc
    31801 aagtcgggcg agatcgtcgc aatcataggg gggactggtt caggtaaaac gaccttagtc
    31861 aatttgatcc ctcgattata tgacatcgaa tctggttcga tccaaataaa tggaacggac
    31921 attagtgaca tgactcaata taatttgcga gaattaatgg gctttgttcc tcaaaaagca
    31981 gttctgttct ctggaacgat ccgagacaat atgcagtatg gtaagccgga tgcaacagat
    32041 gaaatgattt ggaaagcatt agaaatcgca caagctaaag attttgtttc agagatggaa
    32101 gatggcttag atagccatgt ggaacaagga ggaggaaact tctctggtgg acaaagacag
    32161 cgtctggcaa ttgcacgggc attagtcaaa acagcagata tctatgtgtt tgatgattcc
    32221 ttctcagcgt tggatttcaa aacggatgcc aatcttcgcc aagcattgaa aacgaatatg
    32281 aaagaaagta tcaccgtttt agttgctcaa cgagtaagta cagtaatgga tgcagatatg
    32341 atccttgtgc tagatgaagg aaaactggtt ggcaaaggaa cacatgaaga actattagca
    32401 acaaacgaaa cttatcaaga aatcgttcat tcacaactaa gagaggagga cttagcatga
    32461 gtaaaacaaa cacacgtccc tctcatggac cagcaggcgg tccaggacat ggtggacctg
    32521 gcagaaatct cggtgtaaaa ggtgaaaagc ctaaaaattt ctggggaacg gtacgccgat
    32581 tgtttggcta tatgtctaaa cgtatggttg cgatcatcgc tgtatttgta ctagcaatag
    32641 cagccgtgat cttccaaatc caaacaccaa aggtcttggg gaaagcaacc acagaaatat
    32701 tcaaaggtgt aatggctggc gctcaacaga tgcaacaagg ccaacaagta acaaaatttc
    32761 cgatagactt tgataaaatc gcgcagatca ttgcgacagt aatcgtgatg tatttgattt
    32821 ctgctgtatt caacttttta cagcaattta ttatgacacg tgtttcccaa cgaacagttt
    32881 atgaattacg tcatgatttg gaagccaaaa tgaatcgtgt accgatttcc tattatgata
    32941 cacatacaaa tggggatatc atgtctcgag caatcaatga tatggataac atcgcaagca
    33001 cgctgcagca aaatttgact caatttgtaa ctagtgcagt cacattgatt ggggtcatcg
    33061 tgatgatgct ttcaatcagc tggcaattga cgttagtcgc gttattgatg atccctttaa
    33121 gcttgatcat cgtgatgatc gtagcgccga aatcacaagt gtttttcgca gatcagcaaa
    33181 aaagtttagg tttattgaat aaccaagtag aagaaacata tggtggtcat accattgtta
    33241 aaacattcaa tcatactgaa aaagaccagg aagtatttga aaaagaaaat cagaatctgt
    33301 atgccgctgg ttggaaagct caatttattt cggcaatcat catgccgttg atgaatttcg
    33361 ttaaaaatct aggctatgtg ttcgtcgctg tactaggcgg agtaaaagtt gcaaatggga
    33421 acatgacatt aggggatgtt caagcgttct tgcaatatac aacacagttc tctcaaccaa
    33481 tcactcaact tgcaagtttg atgaacacta tccaatcaac agttgcttca gccgaacggg
    33541 tatttgaagt gttggatgaa gaagagatgt ccaatgaaaa atctggcctt ccagaagaaa
    33601 aagatacacc atacaaagtt cgttttgaaa atgttcaatt cggctacaca ccagataact
    33661 tgctaatgac tgattttaac ttggatgtca aacctggtga gatggtcgct attgtaggtc
    33721 cgactggtgc agggaagacg acactgatca accttcttga acggttctac gatgtttctg
    33781 gaggtagtat tcgttacgat ggtgtagata cccgtgatct ttcacgtgat gaattgcgcg
    33841 cacagttttc aatggtactg caagatactt ggctgtttac aggaagtatc tatgacaata
    33901 tcaaatatgg gaacgacgat gcaacagacg aacaaatcat tgaggctgct aaagctgctc
    33961 acgtagacga ttttgttcgt aaattgccag aaggatataa cactgtatta aacgaggatg
    34021 caagcaatat ttcgcaaggg cagcgtcagc taatcacaat tgcgcgggca ttcttagcaa
    34081 atccagatgt cctgatcctt gatgaagcaa catctagtgt agatacacga acagaaatac
    34141 tgattcaaaa agcgatgaac cgcctattgg aaaaccggac aagtttcgtt gttgctcacc
    34201 gcttgtctac aatacgcgat gcagacaaca ttattgtgat gaatcatggt tcgatcgtag
    34261 aaacaggaaa ccacgataca ttgatggaaa aagacggatt ctatgcagat ctttataaca
    34321 gtcagttttc tgaagaagaa gcttcataga atagtacaaa tttattattt tttttagggg
    34381 ctgtgacaaa agtcgtgtca cagtcccttt ttccgaataa acggtgttca atcagctgtc
    34441 cttcggtatt agctcaaaaa acggcaaaat atgagaagct atttttccgt tttgtatctc
    34501 taatactcag gtcaaaccgc tgattttaca acctcttcct taaacggtgt tctcctgcca
    34561 actcctcggt cacaagtcac aatagtcaac aaacctgaga agctgttttt tgaaattgct
    34621 ccttgtgact cggagttaaa cggcaggctc acaacctctt ggtaaacggt gttcaaaagc
    34681 cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagccattt tcacaaaatt
    34741 tttcttacta ctcgaaacta agcgactttt tcacaacctc ttccttaaac ggtgttcaaa
    34801 agcttgcatc tgcggtaata agcccaacca aacgaaaaat aggaggagct atttttgttt
    34861 ggctgttctt attacttgat gcagaacagc tttttcacaa cctcttttta taagagtttt
    34921 tactcattaa tttacaaaaa acaagcaaaa tgcgaatttt ctcacatttt ttttaacaaa
    34981 atcaaagtta ttgcttgaaa gaagaagaat ccatcgttaa aataaagctt gacgtagagg
    35041 ggagcaagaa atatgaaaga ttttcaattg ggggctgcca cctatttaaa aagtgtagcc
    35101 attcgggtga aagaccgaga taaaatgatt gatttttaca agaacgttgt tggctttgat
    35161 ttgaaaagag aagaaaacga attagccatt ttaggaacag aagaagctgg aagtgaaatg
    35221 ttgctgctgg aagaaagtcc tagagcgaat gatcacatgg gggtgatcaa aaagctgaac
    35281 cgtttttcat tgattatccc tagcacagaa gaaatggcag atatcctttg tcgtatccgt
    35341 aaaatgaatt atccaatcaa agaagcttta gaagatcatg gacgtatggg gattttgctc
    35401 gtagacccag aagagaatga attggaaatt taccatgaag agaataagaa cgaccaaaga
    35461 gaaattccag aaacaatgga tcaagaagca ttattaagaa aatctgaaga aaaattcagt
    35521 aaactatcaa caggttctta ttttgaaaaa atccatttaa atgttactaa cttaacaaaa
    35581 gaacgccaat ttcttaatca tgtacttggt ttgaaggtac ataatgaaga agagggcatc
    35641 catgtattga atgaaggaga tttccatgtt ggtttgacag aagctagcgg aggcgcaatt
    35701 gatttgccta cagatgaagt gattggactt gattttctga aattcatgat cgatactgaa
    35761 agcatggaag gactgattgc tcacttgcag caactatcag ttgatttctt cttagacaaa
    35821 aagcacacag tactcacaat ctatgatgct ataggaatcg aatggtggtt cgtcagtgag
    35881 ccaaaagaaa agaataaaac tgacattcga tgcgacgctt aactttttct ttcttaacga
    35941 ttgtcgcatg tctcgtcatt ttatgaacgg tcatactatc gcttattcta gagatagaaa
    36001 gagaaaatag tataccgggg gaaactgcgc ggtttttcgc gcagtttttt tgtgtaaaaa
    36061 aggttgattc ctcatagatt aagctttaag cttggactgt aagaatctga tagaaaagca
    36121 ggagaaaaag atgtatccac acacgaaaca gcttattttg aaaaagatgg aagaaggatt
    36181 ttatccaggt gtagcagtcg ccttcataaa agaaaatcaa gtagaaacat ttacagccgg
    36241 ctttgcccaa gtcgttcctg aaaagcagct gatgacagaa gatctgttgt ttgatgcagc
    36301 ctcactgacg aaagtcgttg caaccacttc attagtttta cgtctattag aagatggaaa
    36361 gatagaactc gatcaaccgc ttcataaata tcttcctgaa tttgaaaatc agacaatcac
    36421 tttgcgtcat ttactgacac atacttctga tatcaaaaca tggataccaa acagagataa
    36481 gctatctcag gaagaattga tcgccgctta tttgaagcta gaaccaggtg agcaactagg
    36541 aaaagaagta aaatatacag atgccggtac gattcttctt gggttgatgc tagaggaaat
    36601 ttaccaagaa actgcagtgg aaatctttca agaacaagta ttggaaccat taggaatgat
    36661 gaacagctta tttcttcctt ttccttccaa aaaaatcgtt cccacagaac aactgccttc
    36721 gggtgaggtt ttgcgtggac aaactcatga tccgaaagca agagttttgg ctgaacatgc
    36781 aggtaacgcg ggtctcttta ttgatttgaa agacagcatt acttttgtcc aaatgtattt
    36841 gaacaaggga aaaatccgta atggtcgttt tttagaagaa gagacgattg attctttact
    36901 gcaagatcaa acaccgaaaa aaaatggcta tcgttcttta ggatgggact taaaaaagga
    36961 tgtagaagac ggtcatgcga ttctttttca tactggttat acaggtacgt ttttcttgat
    37021 tgatataaaa acacaagaag cgttgatatt tttgtcaaat cgcgtacatc ctgtcgatca
    37081 tcgtgcggag tatctcatat gtcgagatga aattgttgaa aactttttga aagagaaagc
    37141 ggtattaccg aaataatgct ataatagcta tgaaaaatga gaggagtttg aattatgcag
    37201 gaacctatgt ttttaaaacc agtttttcaa gaaaaaattt ggggaggaag tcgtttacgt
    37261 tcagtctttg gttttgatat ccctaacgat aaaatcggag aagattgggc gatcagtgcg
    37321 catcctcatg gtgtcagtgt agtagaaaat ggcgaattca aagggaaaag attagatgaa
    37381 ctatggtcag aacacaagga attgtttggc catccttcag aaccagtgtt tccattattg
    37441 atcaagatct tagacgcaga agatgatcta tctgtacaag tacatccaga tgatgcctat
    37501 gggatgaaac atgaaggtga attaggaaaa actgaatgct ggtatatcat cgatgctgaa
    37561 cctggtgcag aaatcatcta tggtcatcat gcaaagacaa gagaagaact agctgagatg
    37621 atcaaagatg ggcgctggga tgaccttttg aagaaagtac cagttaaaaa aggtgatttc
    37681 ttttatgtac caagcggaac gatccatgct attggtaaag gaatcatgat tttagaaaca
    37741 cagcaaagtt ctgacacgac ttaccgcgtg tatgactacg acagaaaaga tgcgcaagga
    37801 caaacaagag agctgcatat ccaacaatcg atcgatgtaa caactgtccc tgcgaaaaca
    37861 ccagaattac aaatcaaaga agtccgaaaa gggaattcat cgatcgtcac ttatttagaa
    37921 actgagtttt tcaatgtata tgagtgggat attaaaggaa tcacatcttt caaaaaacaa
    37981 gccccttata cattgatgac ggtaatcgaa ggagcaggag accttgtcgt tgacggaaaa
    38041 acatatccat tagaaaaagg aacaagctgt attattccaa atggcgtttc tgagtgggcg
    38101 gtccaaggtg aactagcaat cattgcgtct gaaccaggca aatagttccc agatagctga
    38161 aagcagcaga cggtttttcg tttgctgctt tttctacata aaatccgtcc atcaataaat
    38221 agaagtctgg tgaaaaagat ggaatacaaa tggaaagcaa acaacagcag tcgcgtgtct
    38281 atgaaacaac tgagaaaagc tggacatcac ttaaatgaaa tgatcatgcc aaatctaagt
    38341 ctgtttatcg cttggggaat cttgtcatta gcaacaaaag gtgtgtctgg cgatttgcac
    38401 accacattag aagaggtatg ccattggatg atccagctgc ttttacccat cttgatcagt
    38461 tattcaggcg ggaaatcggt agggggacaa aaaggggcag tcgccggtgc aattgcttct
    38521 ttagggttga tcgctcgttc caatgctcca caaatcgtag gagcgatgat tatcgggccg
    38581 cttgccggca tcgtatatga acaatttgtt tctagattcc atgtgaaatt caaggctgga
    38641 tatgaaatgc ttgtcagcaa cctactagta ggactgactg gtagcgttat ttgtataatt
    38701 ggaatcgttt ttattgaacc agttttaaat agtgttcact tgttgctcgc ctccgttgtg
    38761 tcttggctag tcacaatgaa tctgttacca cttgtcagtc tgatcttaga acctctaaaa
    38821 gtactatttt tcaataatgc aatcaatcat ggtgtattga cacctttagg aatcgaattc
    38881 tcacaaacag tcgggtactc ttccttattt ttgatggagg caaatcctgg acctggttta
    38941 gggatcttac tttctatcct ttgttttgca gaaaaacagg aaaaagtaaa tgcgagtgga
    39001 gcattgatga tccatgcgat tggcgggatt cacgaaatct attttccatt tgtcttgctg
    39061 cgaccgtatc tatttttagc tgtaatggct ggtggggcaa gcggcacgtt gatatttcaa
    39121 tggttagata cgggattagc tgcacctgta tcgcctgggt cactactcgt gatttttgcc
    39181 aatgcgcctt atccacaact agctggggta agtataggga tagcaatcag tacatttgtg
    39241 accttcattt gtgcagcgat actattgaaa ttaccaaata aagaaaaaga gaacaaaacg
    39301 gtcaaagaaa ggggagaaat cttggaacca tctatcattc attccgtcat ttttgcttgc
    39361 gactcaggta tgggatcaag tgctatggga gccgccctgc tgaaaaagaa attagcagca
    39421 gaaaaaatcg atctttctgt cggatacagc tctatctatg aattgaataa ccaagtaaat
    39481 cagctggtta tcgtgcaaca tgaactagcg ggtcttgcaa gagaaaaagt acctcaaagt
    39541 caaatctatg tactggatca ctttttggaa acagatatct tgctgtcacg aataaaagaa
    39601 gcgatccaat ttccaaagcg ggaagaaaaa aaagagatag cagatatcac agaacaaaaa
    39661 acgtcgcctg ctaatattgt atttttgtat aaaaacaaca gacgcggctc acaaactatg
    39721 ggcatgacga ttttaagtca attagcaaaa aaagcgaaaa aacagtttgt tatctcaaaa
    39781 gaatcaatag aaaaaatcaa aatccaagaa gaaacgattt atatcgcaca aagagaacta
    39841 gctgtaactg aacagctaga agaaaagatc cctcggctag ttctagttga acattgggta
    39901 gctacgaatg aatacgaaaa actcgtgaag ggagaaatat aagatgtatt tatcgcccag
    39961 agaaaaacaa ttattagcag aatttctcag tagcccgtca cctgtatcta tccaaaaaat
    40021 gatgaattta ttgaaggtca gtaaacggac tatttaccgc gaattagata atcttgaagt
    40081 ctcattgaaa tcggtaaatg cttctttgaa aaaagcagct agaggctcat ttgtcattga
    40141 ggcatcagaa gaagtattag agcaattaaa agaaaataca gatgggaaaa gtaatgactt
    40201 atccactgct aaacgacaac acgcgatttt agccgagttg ttactgacga cagagccgtt
    40261 gagtcttgct catttcatgg aatcatatcg catcagtaat accacttttt atgcagatat
    40321 caaacagttg gaagaaagta ttcgccaact gcctttggaa atcattcgga accagggata
    40381 cgagatcgaa ggaccggaaa aataccgccg gcttctgaca gcgaatgtat tagaattaga
    40441 aatcaatgaa tatgaacttt ttcatagtat ttctttcgat gcatcactga attatttttt
    40501 tcgattcgtt gatccgcaac atctatctct tgcacgcaaa gtaatcgggg aagaattgat
    40561 ccaaaaaaag accaatctaa gtgatagaaa gcttgaacat ttggttttga tgctgacatt
    40621 gacgatggag cgtgtgacga aaaatcattt actgacaaat gagacatata atagtctggc
    40681 aaacaaagaa ttactacaga cagctaaacg attgttttca cggatcgctt ctgagacaaa
    40741 acaactttat ccagtaaatg agatcgtctt ttttgcagga ttattaaatg acttcacgaa
    40801 ctcatttgat cagaatttct ttgaagagac gttcgataca cagcttgctt atttagtaaa
    40861 acaactgatt caagaagtaa gtgaagagac gaatgtccat ttttatgaag atgaaacttt
    40921 gtacaaaatg ctgttgactc atttatctgg tgtgttttca agagccgttt tacaagaaga
    40981 gacactgacc aatccgattt tagaaaaaat catggttcaa tacgaggaag ttgcgaatgc
    41041 aatccgtaaa gcagtagagc ggatttttca agaaaaaaaa ttatccgaag aagagatcgc
    41101 ttatatggtc cttcattttg cgaattcatt ggaacgaagt ccgaaagaaa gcaatatcag
    41161 tattgcagga ttttcgccaa gtggattagc ttctactagc atgttggaac tacgattgaa
    41221 aaggtatttt cccttcatcc aacaaattca tttctatcgg attgctgatt taaaaaaaat
    41281 cgatttacaa gaagaatatg atgttgttat ttctacatct attttaccag gatacggtgg
    41341 aaaatatatt ttagtctccc cgctactgct agaagatgaa gtcaagcagc taaaagaagc
    41401 tttttcgcat attgagaaga aaaaaaggca aaaaattacg tataacagca ataacgagtg
    41461 gatgatagaa gatacttatg aggaaacaat gcatttcatg gatcaaatca atcaattact
    41521 cggtcgtttt tttatccaag aactggataa tcctgagacg atttccgaaa cgattcgcag
    41581 cttatttcct tattttcctg aaggactagt aacggatgaa gaggtggttc atcgccggct
    41641 gcttaagcga tatgaacaag cacctatcgg tattcctcat acaaatatgg ggttattcca
    41701 tacatccagc tcattgattt ctaaaccgat cttttgtatt ttcaatttga aacatccgtt
    41761 gaccattgaa gggatggata gacagccagt cgatttgtcc cgtatgttag ttatgctggc
    41821 gccttcgcca atccaagaaa aagacgggat tcttttagga aagatcagtg gagcaatcat
    41881 catgaatgac ttgaacaccg agatcttccg ctcaggaaat aaagaaatcg tttatcagtt
    41941 gttagcgaaa atcctgattg aagaaataaa gaaatgaagg tgaaaagatg atacagctac
    42001 cagcagaaaa aatattgctc gatcaatcct atcaaaattg gaaagaagcg ttgaccgaga
    42061 tcggccagtg gatgacccaa caagggggaa tcaaaccggc atatatccaa tcaatgatcg
    42121 aaagacaaga aaaagcgagt gtctatatcg ggaatttcgt agccttgcca catgggactg
    42181 acaaagatga agaagtagta gaagaaggaa tctatttagt ccaagtgcct gatggtgtgg
    42241 actttggaac aaaagaggaa ccaaaaatcg cgacactctt atttgtagtg gctttaaaaa
    42301 aagaacatca gttagcggtg ctccaagatt tagccttttt ctgttcagac atcgaccaag
    42361 taatggcgct cagtgatgcg gaaaccattg aagaagtcca aatgattttt gaacatggac
    42421 atatataacc aatgttcaca gtaaaaaaag ctgccttgca tcaagtaata acttcaacca
    42481 agataaaaat aagaggagct atttttgttg ggaggttctt attactgcgg atgcaagctt
    42541 ttgaaaacta tttattctag aaaaccgttt atttgaaata ggggacggtg acaaggtttt
    42601 tgtcaccgcc cctattattt ttgtgtttta atagctcttt tttatataga tagtgcccat
    42661 acctatgaat aataaaacga tactccatat accaagtgtc aagataccat tcatgaaagt
    42721 aacctgttct gaagctagtg gcgagaaaat atctcctcct atgatcacat tttgaaaatc
    42781 aacataagaa gttggcaagt aatgactgat tgattctatc catccaatcg aagaaaacaa
    42841 gcgatactct gctaagaaaa gacaaagaag tgtacctaaa agattgacta gagaatgttc
    42901 aaataaagca gatagaagaa aactgattgt acttaaaaag agcacccaca acaacaggaa
    42961 cagaaagctc tggaaaagat actcatacat cggcaggatt cgtatctcct gaccaatgat
    43021 cgttcctaca ggataacgga agtctcccaa accatttttg attattacta gtatagagac
    43081 gatcaaaaat ggcatcacga gacttagcat agctgcacca taataaatgg taaaacggat
    43141 gctatgtttc tttaccatac tatccggtaa tagattgact agagagatta cctgctttct
    43201 tttttcataa gagatgatgg aagaaagaaa caaggcggtt atacataaaa tcaaaaaact
    43261 agaaatcatt cctgaaaaaa tattttcgta gtaatttgct aaagagagat gcgctggcaa
    43321 gtcaaaaata gaatatcttt ggatctgatg ttctttcata taacggagca actcgacacg
    43381 tttttgttgt tcaatgattg gaattccttg tctttgacca gaaatcagtc gatccagtat
    43441 atctttttca taatgataag ttgcttcgac catttgctgg acattccctg tgttttttgc
    43501 atgtaagatt gcttcgatca gtttgttgct tttctctaaa ctttccacaa attctttctc
    43561 attctcatct tctttcaacg tttcaatcaa acgttgcatc tcttcatagt tatgattcag
    43621 ttcatcgaat tctgtataag caagtttatg atcaaaaaaa agtgggaatg caaagagcac
    43681 gaccaacaat agaggaaata aaaagtttac tttgtttttt agtaagagat tgcgttcaaa
    43741 tgtaagtaaa gacatagtta cacctcatat aatttacgat aaatagctaa aatctcttcc
    43801 atatttgagg attctgtgac gatttctcca ttttttagaa aacatgcttt agaacaaatc
    43861 gattggatat tcgcgatagc gtgggaagaa agaagaatcg tcttcccctc tttctgccat
    43921 tgtctgatga ggctattcaa gatatcaata cttcctgggt ctaatccgtt caacggttca
    43981 tcgaataaca tgacagatga atcactgatc aagtaagcag caagcaagac atgttgtttc
    44041 attcctagag acatttttcc aatcggcaaa gtaaggtact cagtcatctt caatcgttgg
    44101 aaaacagttt ggcattctac tttgctctgc caatagtatt tgatcaaagt caaataatca
    44161 acagccgata gatagtcgga taactgattc gttgattcta gaaaaaagaa agaccggcga
    44221 taatctgtat aagcagtagg agagatgttg tgaagggaca attgatcata agttgttggc
    44281 agcagttggg caatgccatt cagcaaagtt gttttgccaa atccgttcgg ggcaatcaaa
    44341 gcagctactt ctcctgattc tactaaaaga ttcaattgtc gaatcacacg ttttttacca
    44401 cgtttcatgt tcatgttttt gatcagtaat gtcatagata ggcctccaat ataaaattta
    44461 ataacataat agaatggaaa acatctaaat taaatgctaa caaaagttat tgtttgcata
    44521 ttataaaaaa attaaaataa cagttttatt taaaattgaa gttttctgat accgaaatct
    44581 ttttattttg ctgacatact caaacctttg gcacaaagtt tcgtgccaaa gtacgactat
    44641 ttctcttttt agtaagcgga tacaattaga gtatcaaaag gagagaggga gagacaaaat
    44701 ggaacggacg attcccaaaa acaacatttc gctaaaagcg agggtacaaa aattaggcag
    44761 tactttatca agcatggtca tgccaaatat tggagcactg attgcctggg gtgtattaac
    44821 ggctctattt atcccagatg gttatttacc aaatgaggct ttcgctacca tggtaagccc
    44881 aatgctaact tatctgattc ctttattgat cggttatact ggcggaaaag taattgctgg
    44941 tgaccgcgga tcagttgttg gggcaattgc aacgatgggt gtgattgtcg gaacagatat
    45001 tccaatgatg ctaggcgcga tgatcatggg acctcttggt ggatatgcca tcaagaaatt
    45061 tgatcagcta ttccaaaaac ggatcaaatc cggttttgaa atgctagtca ataatttttc
    45121 agcaggtctt atcggttttg gattagcgtt attaggtttt tctgcgattg gtccagttgt
    45181 cgatgcattg actcaagcaa tggcaaaagg tgtagagatc attttatctg cccatttgat
    45241 tccattgaca agtattttta ttgaaccagc aaaaatctta tttttgaata atgcaatcaa
    45301 tcatgggatt ttgacaccat tgggaactga acaagtagca gaggctggaa aatctattct
    45361 attcttgctt gaagcaaatc ctggtccagg acttggcgta ttacttgctt ttactttgtt
    45421 tggcaaagga gcagcaaaat cttcagcacc aggagccatg atcattcact ttttaggcgg
    45481 gatccatgaa atctattttc cttacgttat gatgaaaccg ttattattct tatcagttat
    45541 aggcggagga atgtcaggaa gtttcatttt ccaattgatg gatgctggac taagagcgcc
    45601 agcttctcca gggtcgatca ttgcgattct agcgatgaca ccgttcaatt cttaccttcc
    45661 tgttattttg ggcgtgattg ttgccgcagc tgtttcgttt ggtatttctg cagtaatttt
    45721 gaaagcggat gcgaaagata caaatgaaga cttcgaaaaa tctgttcaag agacaaaggc
    45781 agctaagcaa gcttccaaag gtgcccaagg tattcctcaa ggagctacat tctctggcgt
    45841 acaaaaaatc atttttgctt gtgatgcagg aatgggatca agtgcgatgg gcgcttcgat
    45901 cttgagaaaa aaagtacagg aagcagactt gcctcaaacg gtgaccaatc aagcaatcag
    45961 ccaactgaca gatgactcga atacattgat cgttactcag gtcgaactac aagaaagagc
    46021 aaaacaaaaa gcaccgagcg cgcgttttgt ctcagtagaa aactttttga attcacctcg
    46081 atatgatgaa atcattcaag aattaagcag caagcagaaa acatcagttt ctgaaattga
    46141 gaatgagcca gcagaagagt ttctttcagc aaacatcaaa caaatcaaag agattctact
    46201 tgtccacgat gatcgttccg gatcagcaac aatggggatg accgtgctca aagatatatt
    46261 agacaagaac catttaacta tcccgttaag aaaagtcaat atccaagatt taaaagatga
    46321 acctgattca ctgatcgtag ccaaagaaag tttagcggaa aaagtacaga tggctgcacc
    46381 tcaatccatt catttatcag tcaacagcat ggtcaatccg caaaaatacg aatcgattgc
    46441 acaatatttg aaacagacag cttaagggag gaaaacagca tgcaaagttt gccattcgac
    46501 atgatcgagc tcaatcgatc ttttaaaacc aaagaagaag cgatccgcta ttgcggtcag
    46561 atgcttgtag aagcagggtg cgttgatgag gaatatatcg atgccatggt agatagagac
    46621 aaaatgttat ctgtttatat ggggaatttt atcgcgattc ctcatggaac agatgaagca
    46681 aaaaaatatg tgaaaaaatc cggcatctgt gtcgttcaag taccagatgg tgtagatttt
    46741 ggtaccgaag aagaagaaaa gattgcgact gttttgttcg gtattgcagg agtcggagat
    46801 gagcatttgc aacttgtaca gcaaatcgca ctttattgca gtgatatgga caatgttgtc
    46861 cagctagcag acgcgctcag caaagaagaa gtaacggaaa atctagcaat tgcttaagga
    46921 gagagaaaaa tgagagcagt acattttggt gcaggaaata tcggtcgagg atttattgga
    46981 gaggttttgg cagctaatgg atttgagatt actttcgtag atgtgaatca cacgatcatt
    47041 gatgctttaa atgaaagaaa agaatacgaa atcgaattag cagatgaatc aaaagaaaaa
    47101 atcactgtaa aaaatgtcaa aggaatcaac aatcaaacaa atccagaaaa agtaatccaa
    47161 gaaatcgctg aagcagattt agttactacc gccattggcc cgaatatctt accttttatt
    47221 gctgagttga ttgcaaaagg tatccaagaa agagaaaagg aaggaagcac aacatcttta
    47281 gatatcattg cctgtgaaaa tatgattggc ggttcagcat ttctagaaaa agaagtgtat
    47341 aaatatcttc cggaaacatc atttactgac aaatatatcg gctttccaaa tgcagcagtc
    47401 gatcgaatcg tcccattaca acaccatgaa gatcctttat tcgtccaagt ggaacctttc
    47461 aaagaatggg tcattgacga tagccaaaga aaaaataaaa aaatccaatt aaaaggggtt
    47521 ttatatgtag atgatctaga accatatatc gaacgcaaac tatttagtgt caatactggt
    47581 catgcgactg tcgcttatac aggtgccttg ttaggttacc agaccattga tgaagcaatg
    47641 caagatgcgt tagttgtggc acaattaaaa tcagttttgc aggaaacagg aagtttgctg
    47701 attgccaaat gggggtttga tgcagagcag catcatgcgt atattgaaaa aatcattcat
    47761 cgcttccaaa acaaaaatat ctctgatgcg atcacacgtg tcgctcggac accattacgt
    47821 aagttaggtt atcaagaaag atttacacgt ccagtgagag aactacaaga acataacttg
    47881 acttgcccac atttgacggc aacaatgggg attattttta attactatga tcctgaagat
    47941 gaacaaagcc gtcaattgca tgaaatgaag atccatgaaa acttggaaca attgattcaa
    48001 gaagtcacag gaatcaatga tccgaaaaca atcgggaata tcaagcaaaa cgtgaaccgt
    48061 tatgccaagc aagtcgctta aaataggata cgaaaaaggg gaatgtgacg aaatttttgt
    48121 cacattcccc tttttcgaat aactaggctg tttgtccttc aagatagggc agatccaatt
    48181 ctttaccgta acgttgttct aaagctaggc ggatcaaacg agctgccagg ttctcatttc
    48241 ttgcaaggat aggtccatgg aaataagaac cgaagacatt tcggtaaatg acaccttcag
    48301 attggtcttc accattattt ccttgacctt gtaccacttt ccccaaaggt cgttcacctt
    48361 cacctaagaa agtccgacca ttgtggtttt caaatccata ataagtttca ttgaattctt
    48421 cgttatggat aacgatatca ccgataaacc ggttattttc ctgacttaac gtgtagtgat
    48481 ccagtgcact gattccttgg atcttttctc cattagctcc catgtagtag tgacctagca
    48541 attggtagcc accacaaatg gctaacatga cgccatcatt ttcgatatag ttgatcaaag
    48601 aatctttctt tttctggatg tcttttgaaa tgattaattg ctcaaagtct tgaccaccgc
    48661 caaagaaaac caagtcatat ttatcagcat caaaaggttc atgtatacta acgatctccg
    48721 tatggcaaga aacccccatt ttttctgcta tatatttcaa catcaaaagg tttccgttat
    48781 caccatatgt gttcatcaaa tttccataaa ggtgggcgat attgagttca taattagcca
    48841 cggttcattc ctcctttgat aaatccttga gcagctagtt ctttacgaag ctgcaataca
    48901 gcagtgtaag tagctaaaat atagacatgc tcagttggca attctttgat tttttcgatt
    48961 acttctggta attcttttgt ttctgtcaat ttattttctg gaatcccggc aacttttaga
    49021 cgtaaagtca tatctgtatg acggtctcct ccagaaataa cagctgggat atccatatcg
    49081 gcaaagtttt caaaattacc atcccaaatc cagctgacat cgattccgtc tgcgtaattg
    49141 gcatttaata aagccactaa agaaaatgga taaggagcta gaccaatcat atcgattact
    49201 tgattgatac ctacaggatt tttgacaagt actaatgtac attttttccc attgatattg
    49261 atgacttctt gtcggccgaa tactttctcg tcatatccta aaccttgttt gattttatca
    49321 ggatcgacat tgaagtactc tgccactgca gtagctgcta aagcattgta gacattatac
    49381 ataccgccaa cttcgattcc gtattcatga ccatcgatca cgaaatctgc tgacacattg
    49441 tccatccgta ccatatcagt taatcgataa tccaattcag gacgtttgaa gtcacaattt
    49501 ggacagtaat atttccctaa gtttgcataa gtgatcattt tataatgcaa gatgtgatga
    49561 caatttggac ataagacacc gtctgtattg taatgagcca tttgttcacg atcttcttca
    49621 tggtcaaagc cataatattt tctaggattg actgtttcga tagaattgaa gattggtgaa
    49681 tcaccgttac tgataatcgt tgcatttggt gactttgcgg caccgtctac aatcatttta
    49741 taggtcgtat agatttctcc ataccgatcc atttgatcac ggaaaatatt tgtaaataag
    49801 aacagttctg gtttcatata ttctgtgact ttgctcaaac ttgcttcatc tatttctagg
    49861 acagcaaact ttttctgtcc tttttttggt tttgcctgca agaaagtcga tacaattccc
    49921 tgtaccatat tcgcaccagt agggttggtt agaacttcat cgaattgctg gcgcaaaata
    49981 ttgactgtta aagcggtagt caatgtttta ccgtttgttc cggtgacaac gacgacttgg
    50041 tagtcttttg ccaaactatc tagtatatgt ggatcgactt tcagcgctag tttacctggt
    50101 aaacttgatc cgcctttaaa aaaagttttt aatgcccatt gcgacgtttt tccaactgca
    50161 atcgcaaaat gactgcgtat tcccattatg taacctccaa aaacgatttg ctgacgaata
    50221 aattcaacta atcataccat atgttgaaaa aaaggttaaa ttgcttttct tattcttaag
    50281 aaaaggttac ggctgaaagt gcatgaaatc ggacaaaatc gcatgattta acaaaaaaac
    50341 agcttgaaac aaaatgattg tttcaagctg ctgtgatttt attattccct cttacctata
    50401 ttcccatttt aaccgataat aattttctcg cttggatatt catagcctag atctcgtgtt
    50461 tcttttggca cgaataacat caatgtatat agcattccga tccgcccaat aaacatcagt
    50521 gtagcaatcg tgattttgcc cacaacagac aagtctccgg taatccctaa agataaacca
    50581 gttgttccaa acgcagaagt tacttcgatg atgatcgaga tcagcgtttg ttcttctgta
    50641 gcagataaaa aaacgataca aaagaaacac ataccaagag aaagcataaa gacaacgact
    50701 gattttcgca catcatcttg atcgattctc cgtccaaaaa tattgatgtt atcttcactt
    50761 ttgaggaagg aatatagata caaaccgatg attgctacag tagttgttcg aatcccgccg
    50821 cctacagaac taggactgca tccgataaac atcagtaaag aaaaaatgat cagtgttgtg
    50881 atttgaaaat cacccaaatc gtgaatctgc agaccagcat tacgagtcgt aatcgaataa
    50941 aacatcgaat tgatccattt gacactcata ttggaatctt ggaataaatg gtccttttcc
    51001 aatagataaa tcaagacagt tcccgaaaca aacaagataa caaaagctaa cacagctaat
    51061 ttggtaaaca aggaaaaacg aaacggaagc ttcgcattgg aacgtttgta taatagccat
    51121 tcccggcact ccatcaatac aggaaaacca attcctccga caaaaatcaa aaacatgatc
    51181 aggatcaaga aaaaatagtc atgtgcaaag ggcttgatgg aatccccagt gacgtcaaac
    51241 ccagagttcg tgacagccga tatcgcctga taaaaaccgt aaaaaacggc atcacgccaa
    51301 cgatcaaaat agcctctgta ataaaaataa atagaaaaga atgtcccaaa aagtaattga
    51361 aaccagatta aaatagcaaa agtgatccga atcagtcgga cgataccaga caaacgcggt
    51421 tgattcatat ccgtcatgat cagctgtcgc tgtttcagtg tgatccgtcg tttagaaaaa
    51481 atgacaaaag cagtagaaat catcatgatc cccaaaccac caacttgaaa taatatttcc
    51541 agcagtataa taccgttatc attgaacaca gaattgatat caaaagtcga tagtccagtc
    51601 acactcactg tactgattgc catgaacagc atgtccacaa aagaaacatg gctatctggt
    51661 tcgcgaaaaa tcggcatata aaatagaacg agtgataaga ccgtcatcaa tatataataa
    51721 aaaacgatga tttggatcga tgagaaatga ttggcagcaa acttctgtcc tcgccgctgc
    51781 agccttcgaa agaataaatc aacatgcaca gtactatcct cctgacaata gtcgtttcta
    51841 ccagtataca aggggaatta ggaatactca aagaaattat caaacagata acaatacagc
    51901 tttcgattaa aatagaaatg gcttataata aggaaaatcc tagaaaaaag aaggaggagg
    51961 aataacaaat tgattaatgc tcaacaaatc gtagaaaaaa tgaaaaatca aaaaataaat
    52021 tatgatacag ttctgaaaaa aatgatcatg caatgggaga aagaagaagt aaagcctaag
    52081 atattaatac atacatgctg tgctccttgc agtacgcaca cattggaatt catgtctcaa
    52141 tatgcagaga tcacgttatt tttttcaaat tcgaatattc atcctaaaag tgagtatttg
    52201 cgaagattac atgaacaaaa acgatttgta gaccaattta atgagaaaac tgaccatcat
    52261 gttgcgctaa tcgttgatga ataccgtcca aatgaattca ccaaaatggt tttaacaaac
    52321 catcttgaaa acgaaaaaga aggtggtgct cgctgcagtg catgcttcaa tatgcgatta
    52381 gatcttgttg cgcaaaaagc gcaagaacta ggatacgatt actttggaag tgccctgacc
    52441 atctcgccaa agaaaaatgc agctttgatc aatcagatcg gaatggatat ccaaaagatt
    52501 tacgggacac aatacttgcc aagtgatttc aagaaaaaca aaggctatga acgctctttg
    52561 caaatgtgca aagagtatga tatttatcgc caatgttatt gtggttgcgt gttcgctgcc
    52621 aagcagcaag gatgcgatct gaaagaaatc aatcgggaag caaaagctta cttaaaaaca
    52681 tcaaccgtag tttttgagga aaattaaaga aatctttcgg aaaaacgtaa gaaaaaaata
    52741 aagaatttat ggtagaaata aggtagactg tataatttgt ccgtagtaaa gtaaggaaac
    52801 aagaataaat ttcaatgaag gaagagataa gatgcgaaaa gtagcaccta ttttgaagcc
    52861 aatcgctgta ttaacaatct cgacgattgt cgcgactact acaggtattg tgaatagttc
    52921 cagctaccgt gcaccaagtt caagttctag tactgtagaa gaaacaacac caactacttc
    52981 agtctcgaca tctgaaccaa caacaacgga aagtttgaat gaaaccacta cgacaagtac
    53041 atgggacgat acaacaacta gttcgtcgta tgatgagaca tatacaactg acagctcaag
    53101 cgaaacaagt aatacggtac cagatggcac tccatcggat tcaaccaatc caagtacaag
    53161 tccggataca agtgctagct ccgaaacgat accaagcagt acgactgtag atgataacac
    53221 gcaaaatgat gatgcagcac gtacagcaca gcaaaaccaa gattcaggaa atacccaaac
    53281 gtataaccag caagcaggtg attctcaatg acatttttac tagaccttat caacggggtg
    53341 cataaattcc ttggctattt agacatcagt cctaaatatc ttaaccgtgg atatacgatt
    53401 ttaagtgtga ttcccacgct ttatttattg agaattgttt atggactttg gcaaaaccaa
    53461 aactatcttc aatttttctt atatggactt gcatttctag tgcttgttta ttttactgtt
    53521 ttaaatgtat tttattactt tttagataaa aacacgaaag ccgatgtcac tcagcttttt
    53581 gtgaagtatt tacctgatga agcgttcaac atccagacag aagaatcgat agataatgga
    53641 aattcgatcg ataaagtgaa tacacaagag gtcatggttt cttatgacga agattatcaa
    53701 ttgaaattag ccgagaatat gcggtatttg attggaaatg gtgagatcaa aacaaatgaa
    53761 ctcggtcgca tggatggatt tctggtcgat agaaatacat tgtatcctta ttactatgtc
    53821 aagaaaacag gagagaaaga gtatgttcta aatattggac gaagctactc ggacatggaa
    53881 aaagtaggga ccattcgact tgactcaaca gaagacacat tggaacctgt cggattgttc
    53941 atcgtcggcg gtgattttgt caaagacggg ttacgctatc atgagcctta tcgtctgaaa
    54001 ttgattgcaa aaaaggcaca gtcgcaggaa gaactagtgg tttatagtcg tagccaaaga
    54061 agaagacatt cataatgtga tgaaacgccg tgtatcagtg gttgcgataa aaacgtttat
    54121 tcggaataag ggggatgtga cacgtctttt gtcacgttcc ttttttatat aacgagaaag
    54181 ataagacgaa cacatcactt attctttcct ttcatttata aacattgtag aataaagaaa
    54241 gtacaacgaa gtattgtgaa aaaacgtaat aaataacgag gaaaatacaa gtaggaggta
    54301 agctgtgaaa gaatttttga aaaattgggg gattttgatc cttgtattag cggctatttt
    54361 attagctaga gtgtacgtgt ttacaccagt tactgtaaac gggcattcga tggaccctac
    54421 attaagtgat ggacaacgat tgatttcatc taagatctca aactatgaac gaatggatat
    54481 tattactaca aaggaaccgg gggatgaaga acggatgatt gttaaacgga tcatcggtat
    54541 gcctggagat acagtcaaga tggaaaatga ccagttgacc attaatggca aaaagtatga
    54601 tgaaccttat ctggatgagt tcaaaaaaga attttcagaa gataagttac aaggagaata
    54661 tgcttatagc tcaggattcc aagcacaagc agaaagttca tccaccttta cgagtgactt
    54721 tgaatatact gtgccaaaag gaaagtatct tgttctaggc gataaccgct tgatttcaaa
    54781 agacagccga atgtttgggt tagtcgacaa agatatgatc caaggaaaag ttgttttccg
    54841 ttattggcct ctttcagaaa ttaaaatcat gtgatgaaaa aataaaaaga agctgtgaca
    54901 tgttttttgt cacagcttct ttttccgaaa attccttctt atttcttgga gttgctcact
    54961 ttttttgcaa tctcatttta agctttttgg ctgatggata agaaaagcga agcgtttttg
    55021 ttcataaata gcttttcccc acgatggaag gtatgactag tagcagagac aaagcccgtt
    55081 ttttccaatc gattattcaa ttcttcttgg ctaaagccat tatggacttt cggatgacta
    55141 atttgttcat tcttgtcaaa gtctacgatg atcaattgac cgccaggagc taaaatttca
    55201 taaagttttg taagaatatt ctccgtatca ggaatgtgta aaagtacaag tgatagaagg
    55261 atgagattag cttggacagc aggaaattcg acagaagcat cagcgtggat cgttcggaca
    55321 tttttcagat ctgctgcttg gatcttttct tctgccattt tcagcatagt ttctgaagca
    55381 tcggcaatga tcagttcttt gaatctttcc gcaagcggta aactaacaag cccagttccg
    55441 cctccgtaat ccagcaggac tttttggtgg ctgtcgcgcg gtagataagt agttagttct
    55501 tcgcgaataa tcttggctaa tgcttgacgg tcaggggaat cataatggtt agcgatggaa
    55561 tcaaaaatat tttttgtcat caaatagctc cttacgtaaa actttgaaat tttaacttat
    55621 tatagcacgt gaaaagaaag aaagcatgct atactcaatt caaaaaggga gaatctagaa
    55681 agaatatgcc gatatcgttt gtaaaagata gagaggaaaa aggaaagtgt gtccgagaaa
    55741 tattactaga cttgcctgag tggtttgggc ttcctgaatc aacggaaaaa tatatcgagg
    55801 aatcaagtaa actaccttta tggtgtgaaa aaagaaaaga agaatattta ggctttatca
    55861 ctctttcaca gacaagtgaa gatactgccg aaatctattc tattgtatgg gaataaaaaa
    55921 agactatcat caccaaggat taggaacaaa actttttaaa gaagcagaag gttacgcagc
    55981 aaaacattac aaatatttgc aagtgaagac tgtagacgag gggcactact ctatatatga
    56041 tcaaactatt tgtttctacg aatcattagg attttcaagg ttggaagttt ttccaaattt
    56101 atgggatgaa tggaacccat gtttagttct tgtcaaaaaa ttggaacaaa agtgagcaat
    56161 agatttaata ctaatctgtg cttgatgaaa attctcactg taaaaaatgc tgaaaatcaa
    56221 gtataatacc ataggaacaa actggaaggt gaaaaagagc atggcacaac tattttttaa
    56281 gtatggcgca atgaatagcg gcaagacgat cgagatccta aaagtcgcac ataattatga
    56341 agaacaaaat aagccagttg tcttaatgac aagcggtatt gatacaagag atggtttagg
    56401 cgttgtttca agccgaatcg gactgaaacg taatgcgatg ccgatatttg aagaaacaga
    56461 cgtatttgac gtaattcaga aaatggaaag caaaccctat tgtgtgctga tcgatgaagc
    56521 gcaattctta aaaaagaaac atgtcattgc ttttacgaga attgtggacg aattggatat
    56581 tccggttatg gcatttggtt tgaaaaatga ttttcgaaac gagttatttg aaggatcaaa
    56641 atatttattg ctttatgcag acaaaatcga agaaatgaaa acaatctgtt ggttctgcca
    56701 caaaaaagca atcatgaatt tacattatat tgatggaaac cctgtttatg aaggcgatca
    56761 agtgcaaatt ggcggaaatg aagcttatta ccccgtttgt cgaaaacatt actttcaccc
    56821 atcaatgatt acagaaggag aataagcatg tacgatcaat tacaagcaat tgaagaccgt
    56881 tatgaagagc ttggagaact tttaagtgac ccagaagtga tttctgatac aaagcgtttt
    56941 atgcagcttt caaaagaaga agcaaacacg cgcgaaacag tcgaagttta tcgtcgttat
    57001 aaagaagtag tacaaggaat caccgataca gaagaactgc ttggtgaaaa actggatgat
    57061 gaaatggctg aactagcaaa agaagaatta tctgaattaa aaaaagaaaa agaagtctta
    57121 gaagaaaaaa tcaagatctt gcttttacca aaagatccaa atgacgataa aaatatcatc
    57181 atggaaatcc gtggagcagc aggcggagac gaagcagcat tattcgctgg cgatcttttc
    57241 aatatgtacc aaaagtatgc tgaagcacaa ggctggaaaa cagaagtgat ggaagctaac
    57301 gttaccggaa tcggtggata taaagaagtg atcatgatga tcaccggaga caacgtatat
    57361 tcgaaattaa aatatgaaag tggtgcgcac cgcgtccagc gtgttccttc aactgaatcg
    57421 caagggcgta tccatacttc caccgcaaca gttgtcgtga tgccagaagc tgaagaagta
    57481 gagatcgata ttgaagacaa agatatccgt acagatatct atcatgcttc aggtgctggc
    57541 ggacagcacg tcaataaaac agcatctgct gtacgtttga ctcacttacc aacagggatc
    57601 gttgttgcta tgcaagatga acgttctcaa atcaagaacc gtgaaaaagc aatgaaaatc
    57661 ttgcgtgctc gtgtgtacga taagatccag caagaagcac aaagcgagta tgatgccaat
    57721 cgaaaatcag cagtcggaac tggggatcgt tcagaacgga tccggacgta taatttcccg
    57781 caaaatcgtg tgactgatca tcgtatcgga ttaactatcc aaaaattaga tcagatttta
    57841 gcagggaaat tagatgagat cgttgatgca ttgatcatgt atgatcaaac atcaaaactg
    57901 gaagctatgc aaaatgggta aaatgaccta tcgagaagtc ctgtcacggg cttcttcttt
    57961 tttagaggat catgggaaag aagcatatag tatccaattt ttatttttgg aacgaaaaca
    58021 atggaaaaaa cttgattggc tccttcatat gaacgaagag atttcagaag aagaacagcg
    58081 gttgatcgaa accgatctgc gacttctgct tgcagatcat ccacctcagt atttgctagg
    58141 atatgcagat ttttatgatc atcggttaaa ggtaactgaa gcaacactga tccctcgacc
    58201 agaaacagag gagttagtcg aatggtgttt agacgagacg cctggtgtac cgttagaagt
    58261 gatagatatt gggacaggga ctggagcaat tgctattagt ttgaaagcag caagaaaaaa
    58321 ttggcatata tctgctgtgg atctttcaga agaagcatta gaagtagcga aagaaaatgc
    58381 tcagaaagaa ggaacgaaaa tcagttttta tcatggagat acattaggac cagtaatgga
    58441 tcagcaattt gatgtgatta tttctaatcc gccgtacatc agtcggaatg aatgggaact
    58501 gatggatgaa agtgtccggt cattcgaacc taaaatggcc ctttttgctg agaacgatgg
    58561 attagctatt tatgaaaaaa tagcagttga agcaccaagc gtactaaaat caaatggaaa
    58621 gattttttta gaaatcggat ttcgccaagg tgaagcagta aaaaaaatat ttcagcaagc
    58681 atttccagat aaaaaagtgg cgataaaaaa ggatcttttt ggcaatgaac gaatgatcca
    58741 agtttatggt tgaagttgtg gatgaaaaat tttttatttg tttttttatt ataaaaaaac
    58801 acttatttga caaggatgta aacgactttt tggtttatca acaagtttat ccactattga
    58861 caaatagatt atccacaaac agtggaaaac ttgaggataa actcagacaa aataagaatg
    58921 tatgaaaaag gggttgtgga taaactgtgg aaacgaaacg atatgatgaa acagaattgc
    58981 aagaggcagc cgaagcactg aaagaaggag aacttgttgc ttttcctacc gaaacagtat
    59041 acggattggg cgccaacgct cttttaccaa acgctgtcaa gaaagtgttt tctgtaaaag
    59101 ggagaccgca agataatcca ttaatcgtcc atgtcgcctc ttttgaacaa gtaaaagagt
    59161 atgtggacaa ctttcatccg gcagcagaga agattgtgaa gaacttctgg cctggaccat
    59221 taactttgat atttaagatc aaaaaagaca ctttgccctc agttgtgacg ggaggattat
    59281 ccacagctgc atttcgtata ccggataaca aaaaaacact agaagtaatc gaactttccg
    59341 gtgttccgct tgtcggtcca agtgcaaata cgtcgggaaa accaagtcca acgactgcag
    59401 atcatgtata tcatgatcta caaggaaaaa ttaccgggat tattgatgat ggagctacgc
    59461 gaattggtgt agaatccact gttcttgatt taagtgatcc aacagccatg cctatgattt
    59521 tacgaccagg tgcagtaacg aaagaacaaa tcgaagcagt catcgaatct cctgttgcta
    59581 ttgatcagca tcttgtaaaa gaaaatgaaa caccaaaagc ccctgggatg aaatacaaac
    59641 actactcacc cgatacacgt gttctgatgg tcagagatgg cgattggtca actgcagttc
    59701 aatgggcaaa aaataagaag atccgtgcag gtgtcatagc aagccccgaa attgcggatc
    59761 aagtgcggac agatactgca gctgtttata tgtataacga caactcagta gaagcggcgg
    59821 ctaaaggatt gtttgcaggt ctaagaggat tagatgaacc aacacttgga ttagatttga
    59881 tttttgtaca agtgtatcca gaaactggat tggggaatgc ttacatgaat cgcttgaaaa
    59941 aagcagcagg acaaaattat tttgaaaaat gatcaattgg ttattgtcgc attcttttag
    60001 tatgatacaa tgctatcaag taaatcttct atcagtgaac gaaaaactaa gcgtaactaa
    60061 tcgtaataga agagatacga atatttggca atttgttttt gatgggaaaa ataaaattgt
    60121 aggaaaataa aaaggagctg ttgtttgtgg tagattacaa aacgtttgac cctgatcttt
    60181 gggctgcgat cgccaaagaa gaagaaagac aagaacataa tttggagctg attgcctctg
    60241 aaaactttgt ctccgaagca gtgatggctg cacaaggaag tatcttgaca aataaatacg
    60301 cagaaggtta tccagggcat cgctattatg gtgggtgtga atttgtcgat atcgttgaaa
    60361 atttagcgat tgaccgtgca aaagaattat tcggagcgaa atttgcgaac gtccagcctc
    60421 actccggctc tcaagccaac acggctgctt atctcgcttt ggtagaacca ggggacacga
    60481 tcttaggaat ggatctttcc gcaggcggac atctaacaca tggatcacca gtcaacttca
    60541 gtggcaaaac ctatcacttt gttgcttacg gtgttgatcc gacaacagaa gttattgatt
    60601 ataatgtggt acgtatttta gctagaaaac atcaaccaaa attgatcgta gcaggtgcca
    60661 gtgcttacgg acgaacgatc gactttgcga aattcagaga aatcgcagat gaagtaggcg
    60721 caaaactgat ggtagatatg gcacatatcg caggattagt agcagcagga cttcatccta
    60781 atccagttcc ttatgcagat atcacaacga ctaccactca taaaacattg cgtggccctc
    60841 gaggcggcat gattttgaca aacgatgaag cattagcaaa aaaaataaat agtgcggtat
    60901 ttcctggtat tcaaggcggc cctctagagc atgtgattgc gggtaaagca gtagcattca
    60961 aagaagcatt agatcctgca tttaaagaat atagtgaaca aatcatcgcc aatgcaaaag
    61021 caatggtcaa agttttcaat caagcaattg gaaccagagt catttcaggg gcgacagata
    61081 atcatctgat gttgatcgat gttcgcgagt taggtataaa tggaaaagaa gcagagagca
    61141 ttttggacag tgtgaatatc actgtaaata aaaactcgat tccttttgaa acactaagtc
    61201 cattcaaaac aagcggtatt cgaatcggca cgccagcaat cactactcgc gggttcaaag
    61261 aagaagatgc tgtaaaagtg gctgaactag ttgtcaaagc attacaagca aaagatgata
    61321 acgctcaact agacgaagtc aaaacaggtg tacgcgaatt aactgaaaaa tttccgttgc
    61381 ataaaaaata aaaatttaaa ttccattagt aaacagaaaa gttcaatgtt gggcttttct
    61441 gtttattttt tgggaaaacg gattctttga caatatcggt gatgctctct agtatatgag
    61501 gtgtaatttt agtcttcaca ctaaatctca gggaggaaaa gtcatgactg atcgtatctc
    61561 gttagaaaag gaagcagtag cattcagtga tgcaaatgcg cctcatccgc gtatctatga
    61621 actagcaccg gaagaaggaa gagttttact agaagaagta caagaatcac ctgtgaacaa
    61681 atattccgtc gatattgaag acttgatggt ggatactggc gaatggggaa aactcaatat
    61741 tcgtctcgta cgaccagaag ggaatatgga taaattacca gttatttttt atattcatgg
    61801 agcaggctgg gtttttggca gtgctcaaac ccatgataaa ttgatcagag aacttgcggt
    61861 tcgtacgaat tccgtcgttg tctttccaga atatagtcga tcaccagaag caaaatatcc
    61921 aacagcaatt gaacaaaatt atgctgtgtt gcaacagctg aataaattag ccaagtcaaa
    61981 aaatctggat ctcgagaatc taatcgttgc aggagattca gtaggtggta acatggctac
    62041 agtcatgacg atcttaacaa aagaacgaaa gggcctccca atatccaagc aattgctgta
    62101 ttatccagtc acaaacgctg attttgatac agattcctat catgaattcg gagaaaacta
    62161 ttttttgaca agagaaggaa tgatgtggtt ttgggaccaa tatacgacga atgaagaaga
    62221 gcgtgctgaa atcacagctt cgcctttgag agcaaatata aatgaactaa atgatcttcc
    62281 ttcagcaatg attttaacgg gagaagcgga tgtattaaga gatgaaggtg aggcttatgc
    62341 gcgcaaactt cgtgatgcag gggttcctgt tacacaagta cgcttccaag gaatcatcca
    62401 tgattttgtc atggtaaatg ctatggatca gacaaaggca acgcgtgcag caatggcatt
    62461 atctacttct tggatcaaga aaaaagaata agctttgctt gtccgcagga aacttctttt
    62521 caaaaacgga ttttcttagg aaaaatggcg agtttgacta gtcaatcact tgattttgtt
    62581 ttacaattaa aaagaaacga cagaacaaag gagagcttaa aaaatgggca aatttcaagt
    62641 gatcgatcat ccattgattc aacacaaact aactattatt cgggacaaaa actgcggaac
    62701 aaaggttttt cgtgaagtag tcgatgaaat cgctatgttg atggcatatg aagtatcacg
    62761 ggatatgcca ttggaagaca tcgtgatcga aacaccgatc actgaaacaa cacaaaaaac
    62821 gttgtctggt aaaaaagtag cgattattcc aatcttgcgc gcaggtattg gaatggttga
    62881 cggtatcttg gaattgattc cagctgctaa agtgggacat atcggtcttt atcgagatga
    62941 agaaacattg gaaccacatg aatatttcgt caaacttcca gaagacattg atgcacgtca
    63001 gttgtttgta gtagatccaa tgttggcaac aggcggatca gcaatcatgg caatcgatgc
    63061 attgaaagaa cgtggcgcgt cgaacattaa atttgtttgt ttggtagcag cacctgaggg
    63121 agtaaaagct ttgcaagaag cgcatcctga tattgacatc tatacagctt ctctagatga
    63181 aaaattgaat gaacatggtt atatcgttcc tggactaggg gatgcgggtg accgtttgtt
    63241 tggtacaaaa taagtaaata cgaaaaaggg tgtgacacaa cttttgtcac atcctttttt
    63301 ggtcattttt aacgtttgat gcagaacagc tttttcacag ctttcgtttg catataaaaa
    63361 aaccatccca aaaatgcaga gatggtcaaa ttcaaatctt ttttagttct ttgtagttaa
    63421 cgattactac acccttactt tgcgtcatca agttcgcatt ttctgcagtt tcatcaatct
    63481 caacaacagc ggaatttttc aattgctttg aaatcgttcc agtttgcgac ttgccgtgaa
    63541 ttgtgaacat tacgtgagta ttgatcttga atttttcacc aatttccgat ggtacgatag
    63601 ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc tgcctttcac atcaattata
    63661 ccacgtttta tgaaaaatat catgaaatta aactaatttt ccatcggact ctttacaatc
    63721 agccaaatag agtatataat ttcttttgga taaggccagc ttagctcagt tggtagagca
    63781 acgcactcgt aacgcgtagg tcgtaggttc gatccctata gctggcatta aaagagaaga
    63841 cagatatatc aaggattttt aatccattga tacatctgtc tttttgttta aaggaaataa
    63901 agtatgcttt cttagcaata gagcatgtat tatttctata aaaatttagg agaataaaac
    63961 gactagcaat atagctgtga aatcaaatat cacatgaact gcccaacttg taatgatcga
    64021 gttgctttta atggcagctt ggttgaataa caatcttgcc accccttgta tcaaaaggat
    64081 gtgtgctaca gtgtgtaaaa tatttccatt atcataagta ctgtaatgga taaatccaaa
    64141 gatgattgct gacaagaaac tagcgacagg aatgctgaac ttactagaaa aaatagcgag
    64201 aaaatacact gagaacaatt cttctccaag tatcatgata ggcaaaataa taaataataa
    64261 cgggctgaaa gcctcattta ttggattgcc tgttaaaccc catttcaaaa tttgttgcaa
    64321 aaataccgag actgcggcac tgattactac attgataaaa aaatatttgg ctatattttt
    64381 gacaggagcg acaggtttcg agaacatttt tttcgtatct tttttaccaa aaacaagtgt
    64441 aaatgctata aatccagtga tagttaaaga aaaaatgcta ataatggatg gcgcaaatag
    64501 caaccctaac aaaccgaata caacgacaat agtgcccaat atcccttttt taagttctat
    64561 tttttttcct aaaatgttat tcatgataat aactcctact attcagtttt tgaagataga
    64621 gtcagtatac atgaagagtt aaaataggca atgttttttt atggatattt gtattgctaa
    64681 aaatttatct tatatattca ttttgattat actactttta tttgtaaaaa ttttctagca
    64741 aaaccaagtg cttgcaactg attaagcgta caatttttta gatgttccga gcgtacttca
    64801 atcatttcaa aggagttgct acttaaatcg atatttttta aatcagtatc aaaaaagcta
    64861 gcattttcta aatcattagt agaaaacatt attttagtga gggaacagtc tacaaaatct
    64921 gcgcttgata agacagagtg ctcaaaggta ctttctttta tgtttgaacc agataaattt
    64981 acatagtcca ttttacaatt ctcgaataac gtgttcccaa tataactttc catcaattta
    65041 gtacctgtta atttacaatt gacaaatttc acgcgatgca acatgctttt tgtcaatata
    65101 cagcctgaaa aatcacagtt ttcgaatata cagtcggtaa tctccattct ttgaaaatca
    65161 ttggaagcaa aaaatgcata cttgaaaata gatttgtaaa taattggatg gcagattatt
    65221 tggttttcga tgacctcttt ttgtgaaagg atattataca gttcaaaatc ttcgttttca
    65281 tagatatgaa aaaaggatcc tttattcaag cttgcttcat taactttagg aaaaatcata
    65341 tttaattacc tccattttca taaagtcata ttaacaaaag ctaaaccaac ttacattagc
    65401 agaaaatgag ataggttttc gcctttttcg ataacgatag tgtcgctatc tcttgaaacg
    65461 ttcttagaat tgccctgaga gacacaaatt aggcgtaatt ctaataaaaa tcttagaata
    65521 aaaattttta ctgatttaat ttagaatgtt tttaaaataa aaatcgttgc cataacagtg
    65581 tattgaaagg ttttaacaat ccagtaatga tttaaaaaaa tctagatgta gcgtatactt
    65641 tgtttataga atgtagtggg aagaaatata tttttgggag gaaatcgtga tgtctaaaag
    65701 tgaagaaata gaaaacatgg tactaaaatt atatgatctc gttttaaatc cagagatcaa
    65761 agaaaaggaa cgtatacttt tgatagacgc aaagacaggc ttagaaaaag gtcagtatta
    65821 tcctaaagtc ataaataatt tggaacgtag tttacgtccg ttagcaatca ggggagaatt
    65881 gtctaaacca gtatcaccgt tttatatgga gatatcaaca ataggaaaat ttgaaaaaga
    65941 attaggtcga ggaatggctt cggcaccgat tacttttggg catttataaa aacgagttat
    66001 tcatctcaat gaattaaaaa gtatagtcaa tctcataatg caggctaggt tcatttattt
    66061 gaacagagtc tgtatttttt tattctaaat aaagaaacag atgtttcttt atattgtatt
    66121 ttatccttta attaacacaa aaacagctag tataaactaa taatgttaat taaaagtgta
    66181 ttttatgaaa ctatcgttat ttattaatgt tgcatttttg taagttaaaa attggaaata
    66241 tagattgaaa aaatgaaagc aataagtaaa ataaaatgaa tatatgcgaa aaaataattt
    66301 acattaggaa aaaccttgta aaaacagttt tttggcatat ttatttttta taaatatttg
    66361 taaaataact atcaattttt tagttaaaag attggaaatg agtgggggag gaattgaaga
    66421 aaggattttt gagagatgat atagtaggtt ttatttttag ttaaaatagg ggagatagaa
    66481 aaaatgaaaa agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca
    66541 tcactgattc ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact
    66601 tcgttgaccg tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg
    66661 tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt
    66721 gaaggaaata ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa
    66781 aatgttggaa ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt
    66841 aaaaacctag actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc
    66901 gataccggtg aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact
    66961 gtaaatgtga caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga
    67021 gacatgctac ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc
    67081 acttatgttg aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat
    67141 ccaagtacgt ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa
    67201 gagggaatcc aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact
    67261 gataattaca ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg
    67321 agttataaga cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct
    67381 tggtttaaag aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa
    67441 aatatttcag cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa
    67501 tatatcaatg acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat
    67561 gaacagccta ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct
    67621 agtatcaaag gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg
    67681 attgattttg atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca
    67741 gagggagtca gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa
    67801 atttggaacg gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt
    67861 acaaacaatt gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact
    67921 tcagtgactt gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa
    67981 gtgcaagaag tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa
    68041 aacgggctag tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca
    68101 tgggaagaca atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta
    68161 gccgacggtc aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt
    68221 ttcacaaatc tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac
    68281 aacgtgcccg aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg
    68341 gaaaaaacaa atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag
    68401 aagacagaga gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat
    68461 acaggagaat ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag
    68521 ataacaaata aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct
    68581 gtaatcattg gtttattgat agtaactatc tgtgctggga tattcttaca aaaatataaa
    68641 aaagtttagt tagaatataa tattttttgt taaatatgac tgaaatcaaa agaataaaat
    68701 tccgatttat aatcgaaaaa agaagcattt ttatatatgc ttcttttttc gtagaatcaa
    68761 taaatatgga tttggctaac tatggaagaa tactcgtatt agttttcagc gttcatacta
    68821 gttgttacat tgtttgattg gccaaaagaa tttctgatat attttgtgct aaataaagaa
    68881 aaagacgctt gaattgggaa ataagaaaac tactctatca ttacaaattt tcataaactg
    68941 gcagatgttc taagtaaaca agtatctttt caaagtcgga aaaatccata atcaacgtag
    69001 tggtattgat atttggatga aagcctacgg tatcttgacg atcaatagca gcatcgatca
    69061 cgacttggat ttttttctct ttgtcgtgca tcaatgctaa tggagtcact gtacctgctg
    69121 gaacgcctag gaggtcagga agctgttctt cagaaacaaa agataagcgc ttttctttta
    69181 attgttcagc tagttgtttt aaatcggcgt gcttttcatc aggcaagatg acaagataga
    69241 tttgttttcc ttttcttgat ttcaataata gattttttac ttgcggacca ggtagggaaa
    69301 aagggacatt tttgactgac gtaatcgcag gatgatcgac tttttgaaaa gagataccca
    69361 gtttttttag tagttcataa gcttcttgtt cggttgcacg actcatcgcg catcaccctc
    69421 ctttttttat tagtgtatca taaaaaagag ttgacaaatg aaaaataatc atgtaaatta
    69481 cacgtaaagt taaaacaaat gacatcgaaa agaagagtag ccgttaggtg tttttagaga
    69541 gtctctggtt ggtggaaaga ggcagcagtt aaccgtgaaa cacatcttgg agtcgatttt
    69601 ctgaagcgta tagtaggaaa atccggggag cccgttacag ctgcggtcgt tgctagatga
    69661 ccagataagg ctggcttttc cgtgaggagt cggtaaaaaa aggtggaacc acgtgattaa
    69721 cgtcctttga tcttcggatc aaaggacttt ttgttttcat ctgaacggca acgactgtgt
    69781 gaggtttttt ctgcaaggaa aaaataaaca gaggtggaac cacgtataat acgtcctctt
    69841 ggcatttgcc aagaggattt ttttatttta aaggaggaaa agaagatgga gtatttgatt
    69901 gaagttatgc cgcaattatt agtaggagca ctcacaacat tgaaattatt tttatttacc
    69961 ttgattggct cattaccatt agggattgtt ttagcattag gattgtcttt gcattttaag
    70021 ccgctacgct acttgcttca aacatatgta tgggttatgc ggggaacacc gctgttgttg
    70081 caactgattt ttatttttta tgggttgccg acgattggga tcgtttttga tcgtttcgaa
    70141 gcagcactca ttgcctttat cttaaactat gcagcttatt ttgcagagat ctttcgtgga
    70201 gggatacaat cgattcctca aggtcaatat gaagcagcgc aagttttgca attaagtccc
    70261 tttcaaacgg tgacgaaaat cgtcatccca caagtgacaa agattgtttt accttctgta
    70321 gggaatgaag ttatcaactt ggtcaaagat tcttccttga tctatatatt aggtttaggt
    70381 gacgtaatgc gtgctgggaa aatcgcgatg gaacgcgacg cgactttact gcctttgcta
    70441 ggcgtggcaa tcatctatct tacattgact ggtattctaa caatcgtaat gaaacaagtt
    70501 gaaggaaaat taaattatta ccgctaactg ataggaggaa gaaatatgat tgaattgaag
    70561 aaagtgaaca aagcatttgg tgaaaaaaaa ataatcaaag atttgaatct aatcattcca
    70621 gatgggaaaa cattagctat cgtaggtcct tccggtggcg gtaaaaccac gcttttacgt
    70681 atcttggctg gcttagaaag ttctgacagc ggagaatttt atttagacgg tgtaccgttc
    70741 aacccattcg agcttaaaat gaaagaacaa gttgtaggag tcgtattcca agacttccag
    70801 ctatttccac atctgaatat ttttgaaaac attaccttag caccaaaaat ggtattgaaa
    70861 cagacaaaag aagaatacac caaaaaagca gaagaattag cggcttcatt agggattagt
    70921 gatttattga atatctatcc ttatcagctt tctggcggac aaaaacagcg ggtagcgatt
    70981 gccagagcat tggctatgaa tccacgggta ttggcttatg atgaaccaac aagtgcttta
    71041 gatcctgaat tacgccagca agtcgagcgt ttgatcctat cattgaaaga agatggggta
    71101 acccaaatta ttgtcaccca tgatttagta tttgcagaaa atgtcggtga tcaattacta
    71161 aaagtttccc caattcatta agaaaggaga agatagagat gaaaaaaacc agttttctat
    71221 tcgctttgat cgcaggtctt cttctttttg caggctgttc gaacaaaaaa acttcagcag
    71281 ataattgggc gactatcaac gaagagaaaa aaatcgttat aggtttagac gatacatttg
    71341 ttcctatggg atttagagat aaagacggga agctcacagg atttgatatt gatttagcca
    71401 aagcagtctt tgaacaatat gggattacgg tagactttca gcccattgac tggtcaatga
    71461 aggaatttga actgaacaat ggaacgatcg atttgatatg gaacggctat tccaaaacgc
    71521 cggcaagaga aaagaaagtg caatttacaa aaccttatat ggaaaacgat caagtgctga
    71581 tcacacctaa agaatcagga atcacaaact ttggacaaat gaataataag agattaggtg
    71641 cgcaaaatgg gtcatctggt tatgatgtat ttacgaaaca accagaagta ttaaaagaga
    71701 tagtcaaaaa ccaagatgct gttttgtatg atagttttaa tgaagcattg atcgatctga
    71761 aaagcagacg ggtcgatggt ctgctgatgg ataaagtata cgcagactat tatttgaaac
    71821 aaagaaacga actagaagac ttcaacataa caaaaggttc ttatgagagt gaggattttg
    71881 cagttggtgc acgcaagtca gatagtacat tagtgaaaaa aatcaatgaa gccctagatc
    71941 aactagagaa aacaggtgaa ttccaaaaaa tatccgataa atggtttggc gaagatgtta
    72001 cgccgagaaa ttaacaaata ggggactgtg acaaaagacg tgtcacagtc cctttttccg
    72061 aataaacagt gttctcctgc caactccttg tgactcggag ctgaatggca ggctcacaac
    72121 ctcttggtaa acggtgttca aaagccagtt tttcggtatt aagccaaatt tatgcaaaaa
    72181 tgtagaaagc cattttcaca aaatttttct tactactcga aactaagcga ctttttcaca
    72241 acctccttta catattttta ttttgttgtg gaaggggttt cgtgttattc taaaagagga
    72301 acgattgaag gttataggaa aggaagagat gaatggaaca caaagaactt acctcatttc
    72361 caaaagattt tctgtggggc tctgcttctg cagcgtatca agtagaagga gcttggcaag
    72421 aagatgggaa aggagaatca gtctgggatc gatttgtacg gatacctgga aagacattta
    72481 aaggaacgaa cggtgatttg gcagtagacc actatcatcg ttataaagaa gatattgctt
    72541 taatgaaaga acaaggtctg aaagcttacc gcttctctgt agcttggaca cggatttttc
    72601 ctaatggacg aggagaagtc aatcaagctg ggctcgcttt ttatgagaga ttgatcgacg
    72661 aattgattga aaacgatatt gaacctatgt tgactttata tcattgggat cttccacaag
    72721 cattgcaaga tgaatataat ggttgggaat cacgtcagat cattgaagat ttcactcatt
    72781 atgcagaaac attatttgag gcattccgcg ggaaagtcca ttactggatt agtttgaatg
    72841 aacaaaatat cttcacttca ttaggttatt tgttagcagc acatccgcca ggtgtgacag
    72901 acccgaaacg aatgtatgaa gttaaccata ttgctaattt agcaaatgca tctgtcatca
    72961 ataaatttca cgagttagaa atgcctggaa aaatcggacc aagctttgcg tatacgccaa
    73021 attatccaat tgacagtaat cctgaaaatg ttctggctgc tgaaaatgca gaagacctga
    73081 tggccaatta ttggatggat gtctatatgt ggggaaaata tccgattgct gccatgaggt
    73141 tcttagaaga aaaaggatgg gcaccaacga ttgaagctgg cgatgcagaa ttgctagaat
    73201 ctgctaaacc agattttctt gggatcaatt actatcaaac ggcgacgaat gcattcaatc
    73261 cattaaacgg agttggtgct ggaaaaatga atacgacagg aaaaaaaggt tcatcagaag
    73321 aaactggagt tcctggaatg tataaaaaag ttgagaatcc atttgtcgaa cgtacaaatt
    73381 gggactggga gattgaccca gaaggattaa ggatcggact gcgtcgtatc acaagccgtt
    73441 accgcatgcc ggtactgatt acggaaaatg gcttaggaga gtatgacaag ttgacagaag
    73501 ataaacaaat ccatgacgac tatcgaatca attacttaca aagccacgtc aaagccatca
    73561 aagaagcaat ttcggatgga gcagaagtcc taggctattg cacctggtct tatacagatt
    73621 tgcttagctg gctgaacggt tatcaaaagc gatatggatt tgtatatgtt gatcaagacg
    73681 aaacacaaga aggatctttg gaacgatata agaaagacag ttattactgg taccaaaaac
    73741 tgattgaaga aaatggagag aacctttagt tcacatactt aaaacaagaa ggtacggctg
    73801 gatatggccg tgccttttct tgataaaatc atttagaaat taagaaaaac tgtaaaataa
    73861 gctgaaaaat gaaagaaagc gtcagttctt tcaaacaaaa ctttagttcc cttacttttt
    73921 tttgtataat gaaacttgat gaaatgaggg ataataaatg gcacaaaaga aacggcctac
    73981 taaaaaaagg aaaaagacga aaaaaacgaa aaagcaaaaa cagcagcagg aacaaatgct
    74041 gacgattggt gttgggtttg ccttgatttt atttgctgtc ttcggctttt taaaattagg
    74101 ttttttagga atattgattg caaacggctt tcggatcatt gcaggaaata cttaccaaat
    74161 cctctgtctt ttattagccg tattaggatt ttggattgtc attaagaata cagagttttc
    74221 aattggtaaa aaccgacgct ggtttggcgg catactcttt tatttcggta tcttattatt
    74281 gttacatgcc catttatttg gcaaactcca tactggggaa ccaaatatca tggggacgac
    74341 atgggatttg ttagccagtg atataaaaca aagtcaagta gacaataacg ttggtggtgg
    74401 gatgattgga gcaatccttt atcattttac ttacttcttg attgcacagc ctggaagtta
    74461 tttagcagca gtgctattac tggcaggagg agcattcctg ttcagtaatc tggaaggcta
    74521 tcagctgtta aatggtatcc aatcaattgg agaacgagta caggaattat tggaaggtga
    74581 tcctgccaag caagctcgaa agcaagcggc aaaggaagag agaatgaaac agcgcgcaga
    74641 agcgaaagaa gcaaggcgct tagctgcaaa agaagctgca gaaaaagaag ctgcagaata
    74701 tgagaagaaa aaagcggtga gccagcaaag ggaaaatact gctgatgaat ggcaggaacc
    74761 agaaaataca gagccggagc agctaagttt tgttccaatc gacagttttc aagaaaatat
    74821 ccatcctgcg aatctagaga aaccagtacc agatacacca aaacagacca atacagcaga
    74881 aggtttttca gatgagttgc cagaggatga cggtacctca ttagaatttg agattgaagc
    74941 agaacaggaa aatcaagact atgaattacc gacagttgat ttgcttgatt ctataccaac
    75001 tgtggatcaa agtgatgagt ataaaaaaat tgaaaaaaat atcggcgtat tagaacaaac
    75061 tttccaaagt ttcggtgtcg atgccaaggt cgtcaaagcc agcttaggac catccgtcac
    75121 aaaattcgaa gtccagccgg ctgtaggggt aaaagtaagt aaaattgtga acttaacaga
    75181 tgatatcgcg cttgctttag cggcgaaaga tgtccggatg gaagcaccga ttccaggtaa
    75241 atcactgatt ggtattgaag taccaaatgg aaagatcagt atggtttcct ttagagaaat
    75301 catcgaagca caaccaaacc acccagataa attactagaa gtacctttag gacgtgatgt
    75361 ttctggacgg gtacaaacgg ctgatctttc gaagatgcct cacttactcg tggctggttc
    75421 gactggtagt gggaagtcag tagcaattaa tgggattatc acaagtatat taatgcgtgc
    75481 taagccccat gaagtaaaac tgatgatgat cgatccgaaa atggttgaat tgaatatgta
    75541 caacggcata cctcatctgt tgacgccggt cgtgacgaac cctagaaaag cagcgcaagc
    75601 cttacaaaaa gtagtacaag aaatggaaga acgatatgaa aaattcgcgg caaccggagt
    75661 aagaaatatt tctggttaca atgaatttgt tcaacagaaa aacttggaaa acggaacgaa
    75721 acatccaacc ttaccattca tcgtagtgat cgtagatgaa ttagctgatt tgatgatggt
    75781 agccagcaat gaagttgaag atgcgattat tcgtttagcg caaatggctc gggcagcggg
    75841 gattcatatg atcctagcta ctcaacgtcc aagtgtggat gttatcacag gaatcatcaa
    75901 agcaaatgta ccttccagaa tggcatttgc ggtatcaagc ggtacagatt ctcggacgat
    75961 cattgattct aatggggcag aaaagctctt agggcgagga gatatgctat tcttgccaat
    76021 gggtgagaat aaaccgatcc gtgtgcaagg agcattcatt tctgaccacg aagtagaacg
    76081 ggtcgttcaa tttgttacag atcagcaaga agctcattat gaagagaaaa tgatgccgac
    76141 agatgaagtt gaaacgacag gtgctccaga acagccacaa gatgagctgt ttgaagaagc
    76201 aaaagcactt gtcgttgaaa tgcaaacggc aagtatttcc cttctccaaa gaagatttcg
    76261 gatcggttac aaccgagcag cacgtttagt agatgaactg gaagctcatg gagttgttgg
    76321 cccgtcagaa ggaagcaagc ctcggaaagt attcattgag caggaagaaa catcagaaac
    76381 gttgccggac gataatgaca tgtaaaactt gtgtgatgaa gggttgaaaa acgaataacc
    76441 aataaaaaga caaggcatga tgataccaaa agaaaaaatg gtttttttcc atcttttcta
    76501 atacggtatg atgtcttgtc tttttagttt ttgtgagatt aaagttctat tcgatttatt
    76561 tgtgaagtat tgcattaagg aaatgaatca tgtatagtat gtagcgaaac tttgagaaaa
    76621 agagggaaaa tactttggaa aaacagtcca tttatggctt gacgaatgaa gaattaatca
    76681 attggtttat agaaaacggc gagaaaaaat tccgagctgc ccaagtttgg gaatggttgt
    76741 atcaaaaacg tgtaagtaat ttcacagaaa tgacgaatct atcgaaacaa ttgatcgaaa
    76801 aattatcgga gcattttatc atcaatcctc taaaacagat ggtcgtccaa gaagcttcgg
    76861 atggtacagt aaaatatttg tttgaactcc ctgataaaaa catgattgag actgtactga
    76921 tgagacaaga atatggcttg tcagtttgtg tgacgacaca agtaggatgt aatatcggct
    76981 gtacgttttg tgcaagcggg ctgctcaaaa agaaccgtga tctgactgct ggagaaattg
    77041 ttgcacagat catgatggtc caacattatt ttgatgagcg caatcttggc gaacgcgttt
    77101 cacatgtcgt tgtaatgggg atcggtgagc catttgataa ttacgacaat gtaatggact
    77161 ttttgcatat catcaatgat gccaaaggat tggcaattgg tgctcggcac attacagttt
    77221 ccactagtgg attagcacac aaaatcaagg aattcgctaa taacggcttg caagtcaatc
    77281 tggctatttc cttgcacgcg ccgaataatg aggtgcgaac atcgatcatg cggatcaacc
    77341 gaagtttccc aattgagaaa ttgatggaag ctgtggatga gtatttagag aaaacaaacc
    77401 gtcgaatcac gtttgaatat atcatgttga atcaagtaaa tgatagacca gagcatgcgc
    77461 aacaattagc agatctatta aaagataaga aaaaattagc atatgtcaac ttgattccat
    77521 ataatccagt cagtgaacat gatcaatatt ctcgcagtcc aaaagctgat gtattgagat
    77581 tttatgatgt attgaagaaa aacggcgtaa actgtgtgat acgtaaagaa catggtacag
    77641 atattgatgc agcttgcgga caattgcgaa gcaagcaaat gaaaaaaaca gttgccaaat
    77701 aatcgtatcc tactcgtttt ttcaattacc ataaaaggag cagttttctt aattaaggaa
    77761 ctgttcaact taaataagag aaaagattct atagcgaaag ctataaagct ttttttgaca
    77821 agaaaaaccc tcatcaagca aagaaatgtc ctcaatttga tgacagcctg cttgatgggg
    77881 gttttagcaa ttatttaact gattttctta tatccttgct ggctttcttg tgtagcatga
    77941 taagtttcta cgtcaagaat ggcctgttta aatttttctc gggtgatttc ataaggcaca
    78001 tgtttaatat catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca
    78061 ttgagattca tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt
    78121 ttatcaaaca aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca
    78181 gatgctccgt gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag
    78241 tgtgccatag cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca
    78301 atgatatccg aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc
    78361 aatgcttgcg taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct
    78421 aatctatccg tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg
    78481 gataaagcat cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct
    78541 gtatcaatga aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct
    78601 tcttcgttat agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga
    78661 aaggtgaaga ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg
    78721 ccaccaccga ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt
    78781 tcaatatttc cataagttga attacccccg taccagagac tggcgacaac ctctatttcc
    78841 tgtgtcaaag ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc
    78901 agcactactt ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt
    78961 ccaatggtat aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt
    79021 tgaaacgggt taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag
    79081 gtgaacctaa gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat
    79141 aagtatccta aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt
    79201 cctcttagta tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa
    79261 ttaagattat tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag
    79321 caaaagataa tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc
    79381 ctgatctttc ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga
    79441 taaataatga aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt
    79501 cgttttttca aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca
    79561 gtgaatttga ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga
    79621 cttggccgga agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag
    79681 aacgaatcgc ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc
    79741 ctcaatacgg tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa
    79801 agaaccaaat aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag
    79861 gaatcacgat ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg
    79921 acctgtatga gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt
    79981 atgaattcta tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct
    80041 gggacaaacc ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat
    80101 aaaaagaggt tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt
    80161 acttcttgaa tttttgtgaa attccaactt atttccgaag aagctacttt tggaacaccg
    80221 tgtaccagag gttgtgaaaa agtcgcttag tttcgagtag taagaaaaat tttgtgaaaa
    80281 tggctttcta catttttgca taaatttggc ttaataccga gaaactgg
//
LOCUS       DACDNO010000006        72434 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDNO010000006 DACDNO010000000
VERSION     DACDNO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.006"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(161..445)
                     /locus_tag="IWI22_000415"
     CDS             complement(161..445)
                     /locus_tag="IWI22_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167835.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="IWI22_000416"
     CDS             complement(463..1353)
                     /locus_tag="IWI22_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9167836.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="IWI22_000417"
     CDS             complement(1373..1591)
                     /locus_tag="IWI22_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167837.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="IWI22_000418"
     CDS             complement(1561..3261)
                     /locus_tag="IWI22_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9167838.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="IWI22_000419"
     CDS             3528..4301
                     /locus_tag="IWI22_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167839.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IWI22_000420"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IWI22_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9167840.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="IWI22_000421"
     CDS             complement(5125..6096)
                     /locus_tag="IWI22_000421"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9167841.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IWI22_000422"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IWI22_000422"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9167842.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IWI22_000423"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IWI22_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9167843.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="IWI22_000424"
     CDS             complement(9051..9875)
                     /locus_tag="IWI22_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9167844.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="IWI22_000425"
     CDS             complement(9848..10528)
                     /locus_tag="IWI22_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9167845.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="IWI22_000426"
     CDS             complement(10551..11489)
                     /locus_tag="IWI22_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9167846.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /locus_tag="IWI22_000427"
     CDS             complement(11734..12585)
                     /locus_tag="IWI22_000427"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9167847.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IWI22_000428"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IWI22_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9167848.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="IWI22_000429"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="IWI22_000429"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9167849.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IWI22_000430"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IWI22_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9167850.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IWI22_000431"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IWI22_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9167851.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="IWI22_000432"
     CDS             complement(19463..20704)
                     /locus_tag="IWI22_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9167852.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IWI22_000433"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IWI22_000433"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9167853.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22615)
                     /locus_tag="IWI22_000434"
     CDS             complement(21731..22615)
                     /locus_tag="IWI22_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9167854.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(23393..24679)
                     /locus_tag="IWI22_000435"
     CDS             complement(23393..24679)
                     /locus_tag="IWI22_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9167855.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(24672..25940)
                     /locus_tag="IWI22_000436"
     CDS             complement(24672..25940)
                     /locus_tag="IWI22_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9167856.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            26192..26653
                     /gene="mscL"
                     /locus_tag="IWI22_000437"
     CDS             26192..26653
                     /gene="mscL"
                     /locus_tag="IWI22_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9167857.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(26712..27158)
                     /locus_tag="IWI22_000438"
     CDS             complement(26712..27158)
                     /locus_tag="IWI22_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9167858.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(27251..27496)
                     /locus_tag="IWI22_000439"
     CDS             complement(27251..27496)
                     /locus_tag="IWI22_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167859.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(27611..28189)
                     /locus_tag="IWI22_000440"
     CDS             complement(27611..28189)
                     /locus_tag="IWI22_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9167860.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(28266..28796)
                     /locus_tag="IWI22_000441"
     CDS             complement(28266..28796)
                     /locus_tag="IWI22_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9167861.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(28800..29660)
                     /gene="rlmB"
                     /locus_tag="IWI22_000442"
     CDS             complement(28800..29660)
                     /gene="rlmB"
                     /locus_tag="IWI22_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9167862.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(29653..30048)
                     /locus_tag="IWI22_000443"
     CDS             complement(29653..30048)
                     /locus_tag="IWI22_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9167863.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(30045..31457)
                     /locus_tag="IWI22_000444"
     CDS             complement(30045..31457)
                     /locus_tag="IWI22_000444"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9167864.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(31454..31990)
                     /gene="cysE"
                     /locus_tag="IWI22_000445"
     CDS             complement(31454..31990)
                     /gene="cysE"
                     /locus_tag="IWI22_000445"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9167865.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(32312..33772)
                     /locus_tag="IWI22_000446"
     CDS             complement(32312..33772)
                     /locus_tag="IWI22_000446"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9167866.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(33942..35099)
                     /locus_tag="IWI22_000447"
     CDS             complement(33942..35099)
                     /locus_tag="IWI22_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9167867.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(35133..36503)
                     /gene="radA"
                     /locus_tag="IWI22_000448"
     CDS             complement(35133..36503)
                     /gene="radA"
                     /locus_tag="IWI22_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9167868.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(36566..37093)
                     /locus_tag="IWI22_000449"
     CDS             complement(36566..37093)
                     /locus_tag="IWI22_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9167869.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            37323..38129
                     /gene="proB"
                     /locus_tag="IWI22_000450"
     CDS             37323..38129
                     /gene="proB"
                     /locus_tag="IWI22_000450"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9167870.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            38126..39373
                     /locus_tag="IWI22_000451"
     CDS             38126..39373
                     /locus_tag="IWI22_000451"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9167871.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            39449..39793
                     /locus_tag="IWI22_000452"
     CDS             39449..39793
                     /locus_tag="IWI22_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167872.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(39910..40236)
                     /locus_tag="IWI22_000453"
     CDS             complement(39910..40236)
                     /locus_tag="IWI22_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167873.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(40609..41810)
                     /locus_tag="IWI22_000454"
                     /pseudo
     CDS             complement(40609..41810)
                     /locus_tag="IWI22_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            41927..42442
                     /locus_tag="IWI22_000455"
     CDS             41927..42442
                     /locus_tag="IWI22_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9167874.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(42641..43087)
                     /locus_tag="IWI22_000456"
     CDS             complement(42641..43087)
                     /locus_tag="IWI22_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9167875.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(43134..44612)
                     /gene="msr"
                     /locus_tag="IWI22_000457"
     CDS             complement(43134..44612)
                     /gene="msr"
                     /locus_tag="IWI22_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9167876.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(44966..45595)
                     /locus_tag="IWI22_000458"
     CDS             complement(44966..45595)
                     /locus_tag="IWI22_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9167877.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(45579..46043)
                     /locus_tag="IWI22_000459"
     CDS             complement(45579..46043)
                     /locus_tag="IWI22_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9167878.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(46530..47798)
                     /locus_tag="IWI22_000460"
     CDS             complement(46530..47798)
                     /locus_tag="IWI22_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9167879.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(48029..48766)
                     /locus_tag="IWI22_000461"
     CDS             complement(48029..48766)
                     /locus_tag="IWI22_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9167880.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(48833..49402)
                     /locus_tag="IWI22_000462"
     CDS             complement(48833..49402)
                     /locus_tag="IWI22_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9167881.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            49530..50402
                     /locus_tag="IWI22_000463"
     CDS             49530..50402
                     /locus_tag="IWI22_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9167882.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            50744..51616
                     /locus_tag="IWI22_000464"
     CDS             50744..51616
                     /locus_tag="IWI22_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9167883.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            51613..53571
                     /locus_tag="IWI22_000465"
     CDS             51613..53571
                     /locus_tag="IWI22_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9167884.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(53822..54673)
                     /locus_tag="IWI22_000466"
     CDS             complement(53822..54673)
                     /locus_tag="IWI22_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9167885.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            54796..55263
                     /locus_tag="IWI22_000467"
     CDS             54796..55263
                     /locus_tag="IWI22_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9167886.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(55526..56008)
                     /locus_tag="IWI22_000468"
     CDS             complement(55526..56008)
                     /locus_tag="IWI22_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9167887.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(56108..56971)
                     /locus_tag="IWI22_000469"
     CDS             complement(56108..56971)
                     /locus_tag="IWI22_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9167888.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            57569..58417
                     /locus_tag="IWI22_000470"
     CDS             57569..58417
                     /locus_tag="IWI22_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167889.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(58588..58929)
                     /locus_tag="IWI22_000471"
     CDS             complement(58588..58929)
                     /locus_tag="IWI22_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP9167890.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            59015..59722
                     /locus_tag="IWI22_000472"
     CDS             59015..59722
                     /locus_tag="IWI22_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9167891.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            59709..60032
                     /locus_tag="IWI22_000473"
     CDS             59709..60032
                     /locus_tag="IWI22_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9167892.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(60121..61158)
                     /locus_tag="IWI22_000474"
     CDS             complement(60121..61158)
                     /locus_tag="IWI22_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9167893.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(61605..62588)
                     /locus_tag="IWI22_000475"
     CDS             complement(61605..62588)
                     /locus_tag="IWI22_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9167894.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(63004..63774)
                     /locus_tag="IWI22_000476"
     CDS             complement(63004..63774)
                     /locus_tag="IWI22_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9167895.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(63767..64702)
                     /locus_tag="IWI22_000477"
     CDS             complement(63767..64702)
                     /locus_tag="IWI22_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167896.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65296..66945)
                     /locus_tag="IWI22_000478"
     CDS             complement(65296..66945)
                     /locus_tag="IWI22_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9167897.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(67140..68036)
                     /locus_tag="IWI22_000479"
     CDS             complement(67140..68036)
                     /locus_tag="IWI22_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9167898.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(68029..68709)
                     /locus_tag="IWI22_000480"
     CDS             complement(68029..68709)
                     /locus_tag="IWI22_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9167899.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(68710..69492)
                     /locus_tag="IWI22_000481"
     CDS             complement(68710..69492)
                     /locus_tag="IWI22_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9167900.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(69485..70402)
                     /locus_tag="IWI22_000482"
     CDS             complement(69485..70402)
                     /locus_tag="IWI22_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9167901.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(70405..70689)
                     /locus_tag="IWI22_000483"
     CDS             complement(70405..70689)
                     /locus_tag="IWI22_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9167902.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(71390..72010)
                     /locus_tag="IWI22_000484"
     CDS             complement(71390..72010)
                     /locus_tag="IWI22_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167903.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(72091..>72434)
                     /locus_tag="IWI22_000485"
     CDS             complement(72091..>72434)
                     /locus_tag="IWI22_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167904.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
BASE COUNT        20829 a        15441 c        12751 g        23413 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcttaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc ttaaacggtg
    22981 ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta agaagctgtt
    23041 tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct cttggtacac
    23101 ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat ttgagaagca
    23161 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctttgat
    23221 tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat ttgttttatt
    23281 ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt ggaggaggtt
    23341 cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg gtttattttg
    23401 gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca gctgccagta
    23461 cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt cgatcgccaa
    23521 ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat tggaaatatt
    23581 gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc ggatcatcag
    23641 cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg tccgtatctc
    23701 cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca aagctgtcat
    23761 cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct aaaatcattt
    23821 ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct tgcggcaatg
    23881 tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct tccaatgcac
    23941 tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct tgctttggag
    24001 ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca ggctccatct
    24061 taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag cacgtataca
    24121 agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg atatgcaatg
    24181 ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta gggtcgtctt
    24241 catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt tcttctgtga
    24301 agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc tgatccgtcg
    24361 tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa gctccttgtt
    24421 tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt tctaaaaaat
    24481 gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata aattcgttat
    24541 cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca tttttaggca
    24601 acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca ttgatttgct
    24661 catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc ctgcaattcg
    24721 atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg tttcaaccat
    24781 tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc ttcgattgcc
    24841 gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt tgtctgagac
    24901 agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg aatcagatga
    24961 agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa tcctcggaac
    25021 gtatccacgc tactagaggc ataataagct agactttctt tctctctgac attcatgaaa
    25081 agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc aaaacgttct
    25141 ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg aacgattggc
    25201 tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa cagatctgga
    25261 taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc tttgtccgga
    25321 tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc ttgatagtag
    25381 gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa ccctgggatc
    25441 ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc aaaagtttgt
    25501 ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa gtcaaacgtc
    25561 aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt taaaaaatct
    25621 accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa actgatccct
    25681 gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct tgcgccataa
    25741 agctctgcaa gttgcgcact taactttgtc tgatccggat aattcaaact atttgtttcc
    25801 aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg cgcagtaaaa
    25861 cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag attcacgcct
    25921 tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc ccgcttactc
    25981 ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta taagttcagg
    26041 caattcgccg ttttttctaa atgatgttac actttatagt atagcataga tgttttttat
    26101 caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt ttcgatatac
    26161 taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag agttcatcat
    26221 gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt ttacaagcat
    26281 cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg tcgcaatgat
    26341 cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc cgacaaaagg
    26401 cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga tcactggttt
    26461 tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct ccaaagaaga
    26521 gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca tacgcgatct
    26581 tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa atctttccga
    26641 tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt cggctttctt
    26701 tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg aatatcatct
    26761 tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc ttgtaggtat
    26821 tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat gttaatcgtt
    26881 tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac aaggacacgt
    26941 ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat cttcccatca
    27001 gtatcttgaa aaaacgtatg ccaagcttgt ccatcaatgt catcaacttc ttgataagga
    27061 cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta aaactctgct
    27121 gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc ccctgctttt
    27181 ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga atctcaggct
    27241 cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga gtaagtacat
    27301 ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa tcaacaacga
    27361 atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc tttcttcctg
    27421 tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc ttgattgaag
    27481 ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat ttttaaaatt
    27541 ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag ctcattaaga
    27601 tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg agttttactc
    27661 tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc atgtctacaa
    27721 aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa aactggattt
    27781 cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt tcttgcaatg
    27841 tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt gagacttcat
    27901 ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag atttgattgc
    27961 ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg taagttctca
    28021 atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca taatttctta
    28081 aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac agggcagcaa
    28141 atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata aaataattcc
    28201 tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact gattagaacg
    28261 atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt ttttaactgc
    28321 tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt attttcttgg
    28381 gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga agttcgtaat
    28441 gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac tgtcacttgt
    28501 gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc cgtttcgtct
    28561 tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat gcccggaact
    28621 agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata atttgataaa
    28681 cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag ctggacaagt
    28741 tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt tttcatggtc
    28801 tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc aacactggca
    28861 tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat ttcttttttc
    28921 aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga tcctttggca
    28981 ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa tcctgtttct
    29041 ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac atgttcgact
    29101 gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg ttttggtatg
    29161 atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa attgtgtgga
    29221 tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt tgttcgttca
    29281 atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac tccttgatgc
    29341 acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac tggcacagct
    29401 tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc ttcttgaaga
    29461 aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg cccaaagacg
    29521 aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg tccagtttgc
    29581 tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga ttgatgtttg
    29641 tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc ggatcaattc
    29701 ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt cgaaccctgt
    29761 tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt tagcatttct
    29821 tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca gcatttgctg
    29881 aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat gcagcttatt
    29941 gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt aaatcgcatc
    30001 tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc ttctcttctc
    30061 catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa caaatcacga
    30121 atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc ttcgatcaat
    30181 tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa gatcgaaagc
    30241 cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt cggctggtct
    30301 agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt tgctgcatta
    30361 aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa agcgatttta
    30421 tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa attgtcaaac
    30481 gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc actgtagcgg
    30541 atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg atcaacgttt
    30601 ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt ttcatcgtct
    30661 tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt ccctgttttc
    30721 gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg cccgccacca
    30781 tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc aatatgccaa
    30841 ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt tgcttgtttc
    30901 cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt acgctgactt
    30961 gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt ttcaaattta
    31021 cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc aaccaacact
    31081 tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag tgtagcaggc
    31141 tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc agcaacttct
    31201 ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac atccgtgaaa
    31261 ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat tgtatcgaaa
    31321 gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg tccgcagaca
    31381 tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct ggtcaatgta
    31441 ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga acgacttttg
    31501 ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg gcacctgcac
    31561 cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc gcagagagca
    31621 tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg ccgcctaatg
    31681 ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca atgacgattc
    31741 ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg atctcaacac
    31801 cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc cggtgctgat
    31861 ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat gtcaaaacga
    31921 tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc gctcgggtaa
    31981 accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc atcttcttat
    32041 aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc cgcgtggttc
    32101 cacttttctt cataatcaat gattatctct gacagctaac gcctgttata cgttcttggc
    32161 tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag accactccca
    32221 gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct cttgtaatga
    32281 tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt aaatgtgcta
    32341 atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca tgcatttgtc
    32401 ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca gtttcttttt
    32461 gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt tgttctagct
    32521 gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact tcttttgctg
    32581 cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg atttctgcag
    32641 catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg attgtctttt
    32701 catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac atgtcagtaa
    32761 atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt tttttcgcat
    32821 cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc aattcttggt
    32881 gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata aagttgaaca
    32941 ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag attgattcgt
    33001 cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata tgtccgaagg
    33061 ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta ttggcaatat
    33121 ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc gcaacagcaa
    33181 aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg atattatcgg
    33241 attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta ttgcgaggca
    33301 cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt tcttcaggtg
    33361 tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt tgtgcttccc
    33421 gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg ctagcaagca
    33481 gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa gggccgtatt
    33541 ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg ttttccaact
    33601 ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata cgaataataa
    33661 agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt gcatttccaa
    33721 tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc attttcttcc
    33781 tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg gcttctcaaa
    33841 tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa atagtaaagc
    33901 caactggttg aatttcctta ccagttggct gaatttcttt tttattttga ttgtttagtc
    33961 tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg tttagcaaag
    34021 atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat acgttcattc
    34081 atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta ggctactcct
    34141 tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc agggatgacg
    34201 actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt ttggaattca
    34261 gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag cttagccaat
    34321 ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta catttcgaca
    34381 gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg accacgcaca
    34441 cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac aaaattaggg
    34501 atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg accgtcaatg
    34561 atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt tcgttctaac
    34621 agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg tgaaccaaaa
    34681 actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata gcctaagatg
    34741 atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt gtaaagaggg
    34801 gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc gaagagaaga
    34861 taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc tgagatatgt
    34921 ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct gttggtaaat
    34981 gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga gggtaaaaat
    35041 gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg tttttgcatc
    35101 gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt caacgtttca
    35161 gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt acctaagttg
    35221 tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac tcgttgttca
    35281 atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa acattccgtt
    35341 ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc taaatcaatc
    35401 gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg attctgaaga
    35461 agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt gaaatcaaga
    35521 cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc ttggatctct
    35581 accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt tgcaccgtct
    35641 aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc atgctcatgc
    35701 atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc tcgcaaaatc
    35761 cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat atgttctaac
    35821 attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa aatggctatc
    35881 ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg gctgacactt
    35941 cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat gataacataa
    36001 tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc agcgtataga
    36061 taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg ctctgcgctt
    36121 tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg agaaacttgt
    36181 aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat agaccctggc
    36241 accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat ccgaggttct
    36301 tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt caaacttgtc
    36361 cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt ccattttccg
    36421 caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg gcaaacaaat
    36481 tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat gagacattaa
    36541 atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg ccttgacgtt
    36601 gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct tggccgatac
    36661 gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg aacatgatat
    36721 ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact ccgtttgtca
    36781 acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa aattcatctt
    36841 ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt ttttcatttt
    36901 ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc cagcctgctt
    36961 tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca gcagcatgat
    37021 gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata acttcaaatc
    37081 ctctttttct caccggtttt cctccctaaa taaactactc ctattctaac atatccgagg
    37141 ctgtaaaaga ctttctttac aaattcttca agaaattctg ctatcttaaa gtaaaccatt
    37201 aattcgttct ttttccaaac gactgcaagt tgtacaaaaa aactattttt tacatgactt
    37261 tactcgtcaa aacaagtaga atagaacgaa attaatgagt ggatatttga ttgaggtgtc
    37321 acatgcgtaa agaaattaaa aatgcaaaac gaattattgt caaagtagga acaagtacat
    37381 tgatttatcc caatggaaat atcaatcttg cggcaatcga tcagttagct tttgtccttt
    37441 ctgatcttag aaatcgggga aaggaagtga tcttagtctc atcaggagct gtaggcgttg
    37501 gtatgcataa attacatttg tcagaacgtc cgactttgat tcctgaacag caagctgcgg
    37561 cggctgttgg tcaagccgaa ctgatgaata tctataatca aagattcagt gtatataatc
    37621 aacagacggc acagatcctc gtgacaagag acgtggtaga ttatcctgaa agtcgtaaaa
    37681 atgtgaggaa tacattggaa cagcttttga ttatggacac gatccctatc gtgaacgaaa
    37741 acgatactgt agcagtggat gaattagatc atctgacaaa atttggcgat aatgatcagc
    37801 tgtcagccat catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt tctgatatcg
    37861 acgggtttta ttcagataat ccgactacaa atcccgaggc tcagatgtat cgctatatcg
    37921 ataaaatcga tgaaaagatt ctggcacaag caggtggtgc tggtagcaca tttggaacag
    37981 gcggtatgca gagtaaatta aaagccgctt ctaggatatt tgaagcaaac agcgcaatga
    38041 ttttagcaaa tggaaaaaat ccggcaatta tttttgatat tcttgcagga aaagaagtag
    38101 gtactttatt taaggaggac ttgaaatgac tgacttgatg caattaggac aacaggcaaa
    38161 agatagcgcg caacaactag cacttatgcc tacaaaccgc aaaaatgagc tacttgttca
    38221 aatggcacaa accatcaaaa agaatcaaca agcgatcatt gaagcaaatc ataaagactt
    38281 agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc agctgacacc
    38341 tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt gcttcgttag acgatcctat
    38401 tggaaaagta gatgagatgt ggacgaacac cgacggattg agaatcggca aaaagcgcgt
    38461 gccactaggc gtgaccggca tcatttacga atctcgtcca aatgtcacga cagatgcagc
    38521 tagtctagca ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag aagcattctt
    38581 ctcgaatcaa ttattagtcc aattattgca gcaagtactg gtcagtgctg gcgaatctcc
    38641 ttatgccatc cagtttgtcg atgatacttc tcatgaaact gcaggacagt tgatgcgatt
    38701 gacagaatac ttagatgtgc tgattcctcg aggcggagca aaattaatcc aacgagtcaa
    38761 agaacaggca acggtaccta tcattgaaac aggtacagga aataatcacg tatatatcga
    38821 taaagaagca cagttatcta tggcagtgaa catcatcgtc aatgcaaaag cttcccgtcc
    38881 atctgtctgt aacgcggcag aaacattgct gatccattct gagatcgcac cttttttctt
    38941 gccagctatt gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg atgctcgtgc
    39001 gttagaatat ctagagacgg ctgctttagc agaagacaca gattgggata ctgaatatct
    39061 agattatatc cttgctgtca aagtggtcga ttctttggat gaagcaattg cacatatcaa
    39121 tcggcataat actaaacatt cagaaacgat cgtgacagat agttatgcgg ctagtcaacg
    39181 tttcttaaat gaagtggacg cggcggctgt ctatgtcaat gcctccactc gttttacaga
    39241 cggatcggta tttggttttg gtgcagagat tggtatctct actcaaaagt tgcatgcccg
    39301 tggtccaatg gggttaaatg aattaacttc cacaaaatat atcatttatg gcgatggaca
    39361 aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc ttttgccttt tttctgtaaa
    39421 ggttctatcc aactagaaag gtgtcaatat gtttgaaatt ttaatgattt tttttattta
    39481 ccttatttct ctgaatatcg ctgcttttct gggagtcggt attttatccc ttttctttca
    39541 attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt ttgacaaaat
    39601 cgggcaaaaa gggctggtta cacgcttgca cattagttac atggttgcat tatgcttatt
    39661 agcgacaatc aattactatt cttttttcga tcattcaatc gcttatacga ttacgctact
    39721 gattgctggg atttttcact tatcttataa atatcagctg aataaaaacc acttgaatcg
    39781 gactttccgc taaaaaatat ccgtacgata agtagagtgc cttgaagatc ggcaaaaaac
    39841 tgccggactt tttagaggct gtgacaaaga ttttgtcaca gcctcttttt ccatataaac
    39901 ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct tcgttaagaa gcgaaaatcc
    39961 gtcaaatgtt tcgccatcat aataacgaag cagctcatcc atggacaagt tcatcgtttt
    40021 ttctttttct tcttccgctg attcagccag tagctgttcc cgatacacct gatacatacc
    40081 agctattttt tgctgcatcg cttttctagt ttcaccttcc gcattgatta ttggcaattc
    40141 tttgatatag ccacgataaa tcaaccgatc atctgaaaat tcaatgggta attcaacgag
    40201 tgtcaacccg acttcctgaa aatattcttc cagcatctat tatcccccca ttctttttct
    40261 taaatgaatt ttagcttacc tactgaaaat tttccagttt cattattttg agaaaaaaag
    40321 ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa taaatggtgt tcttctgcca
    40381 actcctcaat cataagtcac aatattcaac aaacatgaga aactgttttt tgaaattgtt
    40441 ccttgtgatt cagagttaaa cgacagtctc acaacctctt ggtaaacggc gttcaaaagc
    40501 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatatg agaagctatt tttgtttggc
    40561 tgttcttatg acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc
    40621 atttgtagca aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc
    40681 acctgcaaaa taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa
    40741 tggtgctaaa gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg
    40801 ttgcagacct ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa
    40861 aaatcctgct aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa
    40921 taacagcaaa caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa
    40981 cttccgctca agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc
    41041 aaaaaataca aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc
    41101 aagtaaagta aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt
    41161 atgattgctt aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt
    41221 ttcaggtaaa agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa
    41281 aaatggataa cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat
    41341 tccgcagcca agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat
    41401 actgacgatc gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc
    41461 acgtaaccca atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac
    41521 gatgataaac aatcctgtca tcaatgtttt ttcaaaccga tccgactaga taagaaacct
    41581 gtaaatagca tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca
    41641 gctggcgtag cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact
    41701 cccataaaag cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt
    41761 tcttcatttg tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct
    41821 ctattagcat catacatgat ttattttaca tgtaaatatg cacatgtaaa acgtggaaac
    41881 tttactgtga tataataaag aaaataagac gagaggtagg atttttatga atactttagc
    41941 ctatatatta ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct
    42001 gatcacaatt ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga
    42061 ccagcagggt tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca
    42121 cttgactgat gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc
    42181 tacgcaaaaa gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac
    42241 agctactgga ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca
    42301 aaaaaaattg aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat
    42361 ccagcgcaga attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca
    42421 aatgcagcag ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga
    42481 gcaaattatc ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca
    42541 ggccaaaacc tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca
    42601 aaaagcatgc caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg
    42661 cttttagcac acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa
    42721 tcacattcat tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt
    42781 ccatgatcac accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa
    42841 tagttaagat catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt
    42901 tcgccagcac accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta
    42961 gaccaatcaa caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca
    43021 tccccaagac catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg
    43081 taggcaatct gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa
    43141 tttctcgtaa gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta
    43201 cgctcgacaa agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt
    43261 agcttctcta aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc
    43321 aacacattac ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca
    43381 cttaatgtac ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa
    43441 ctacggacaa gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct
    43501 tcagatgtca tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga
    43561 aggattcctt ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt
    43621 tcaccgacaa tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat
    43681 gttctcccac ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt
    43741 tcaagagatt ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct
    43801 cctatttttt ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca
    43861 ctgtcttttt gtttagagga agacaaccga tccggtctga tttgttgctt tttcttttta
    43921 ctggacactt tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat
    43981 cgttttttct ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc
    44041 ttttgagtga gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact
    44101 tcccagattt tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc
    44161 gtaccataat aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg
    44221 gttgttggct cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg
    44281 atctttcgtt cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg
    44341 aaatgactgg ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc
    44401 aggtagttga aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct
    44461 attagtttga gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct
    44521 tgctgataga cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt
    44581 agatttgtta tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa
    44641 caacaattat cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca
    44701 tcttttccta ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca
    44761 cctattctac tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac
    44821 atgtcttttg aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca
    44881 gtcatttttc cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag
    44941 ctactgaata gtaactgaaa accttttatt cgaacgtagt gatcttttct ggtgctaaac
    45001 gaacagactc gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat
    45061 aacggtcttt atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg
    45121 gatttgtttc atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag
    45181 aatcgacttt gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga
    45241 tcgcagctaa ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt
    45301 tatagatttc ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag
    45361 aagaagtatc attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt
    45421 cctcgctttc agcgaccaca aagcgccatg gttgcatatt gacagaagaa ggtgcagtcg
    45481 tcgcttcttt gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata
    45541 cgcgaacgga tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta
    45601 ttaacctctt tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt
    45661 gttagaaatt gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct
    45721 ttatggtcca tttcacaatg ctcgattttc ttctgggctt tttctgttag cgctacatac
    45781 acttcccggt tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc
    45841 ttcagatgac gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt
    45901 agcaatggat tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg
    45961 atattttctt caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg
    46021 tgactaatct cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt
    46081 gacttgtcaa taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa
    46141 aaacgctctc aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa
    46201 tgataaagaa agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat
    46261 agctcttctg cagtagaaaa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag
    46321 aatcccgtcg aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga
    46381 caagatgttg tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa
    46441 taagttcaac caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg
    46501 atgcagggca gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat
    46561 ctccagccca tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta
    46621 ccagtttttt catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa
    46681 cttagacctt cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt
    46741 gtgtaatgat ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt
    46801 ggtgcttgcc aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct
    46861 ccggcattca atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga
    46921 gaagttgaag ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc
    46981 aatgtagaac cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct
    47041 ttgtaagagt tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa
    47101 gaagtcgtct tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt
    47161 ctaaaagcgg cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta
    47221 acagattggc cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc
    47281 aaagtactgt tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat
    47341 aagtcattgg cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt
    47401 tgagaattag cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac
    47461 aaatctgctt gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct
    47521 tctgcattct ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt
    47581 tctgttgttt cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac
    47641 tgcaaagtac tctctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt
    47701 gcgacagttt ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct
    47761 aaaagaataa cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg
    47821 aaacttttcc taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta
    47881 aataacattt gtttcttttt gataaatttg ctttttctac ataaaaagac gtaaatgaag
    47941 aatattcttc tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta
    48001 attctctttg gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca
    48061 tgttactgac agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat
    48121 cttcggaggt caatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt
    48181 cgctgatggt ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga
    48241 tgttattttg ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg
    48301 ttccgcaata aacggcggaa tcaggataaa caacatgtcc cgccacagaa tcagttgaaa
    48361 tgatatgccc agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca
    48421 cacccatgat gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg
    48481 gagcggttgg catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca
    48541 tcgctaaatc gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat
    48601 atagctcagc ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag
    48661 ctataactag tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc
    48721 ttgaagcgcc catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct
    48781 gttttgaaaa atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa
    48841 tccgttcgac aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga
    48901 catctttcgt aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg
    48961 gcagaatagt tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg
    49021 acaagcctct tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag
    49081 gaccaatcaa tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac
    49141 gattgataaa gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca
    49201 gcagaacagt caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga
    49261 tatctgctgg ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttcagctt
    49321 ggatctgatg gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt
    49381 gtttttcgat gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg
    49441 cttatcatgt ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg
    49501 tatgcttttt taaaaacagg agggaaaaaa tggatattcg tatactgacg tattttttag
    49561 cagtagctag agaaaaaaat atcagccgag cagcaaaatc tctgcatatc acgcaaccta
    49621 ctctctcaaa acaactgaaa gatttagaag aagaacttgg tgtgacactt ttcactcggg
    49681 gctcccgaga gatacagttg acggattctg gtcgatatct ttattcaaaa ggaaaagaaa
    49741 tcttgtcgtt ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc atctctggag
    49801 aaatctacat tggcggaggc gaaactaaag caatctcttt catttctgat agccttcatt
    49861 cgttgatcaa ggcgcatcct gatatccagt ttcatttata tagcgggaat gcagatgata
    49921 tcatagacaa actggatagc ggattacttg attttggact cgtaatcgaa ccgacaaaca
    49981 aacaaaaata ccagtcattt cgactgccag cccaagatcg ttggggactt ttgatacatc
    50041 gaaatcatcc tttagcaaag caaaccgtca tccatccgca ggaactgaaa actgttcctt
    50101 tgatcatttc ccggcaaacg tcagtggaca gtcaactagc tgaatggcta ggtttcagca
    50161 tagatcagtt atcgatcgtc ggaacatata acttattgta caatgcttct ttgatggtcg
    50221 ataacgggac ttttggtgca ttagctctag atgggatcat caatacttat gggacggaaa
    50281 aaatattcat cccactcgat ccgccattac aagcttcact gaatctcatt tggaaaaaag
    50341 accagcctct ttctcctgct gctcgtaaat ttttagatgt cctaacggag aaatacagtt
    50401 aagtagagtt tttttccttc gctttcagct ttttaacaac gaataagata agcaaaagat
    50461 ccaacgatta aataacataa gtttcttaat ttatttcttc cttttatttc tgataattcc
    50521 agtaaattac tataattata aaaaaagaat tgacaaaaca taactgtcta tataaaattg
    50581 attacgttaa caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg
    50641 aggaacatgg tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg
    50701 gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat
    50761 ctttaatcaa aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa
    50821 agggattgga tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat
    50881 atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga
    50941 acgcttttta tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa
    51001 gttgaatgaa catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat
    51061 taataacggt atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga
    51121 tgaattcagt attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata
    51181 tacttatgac gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa
    51241 caataaccat cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga
    51301 atcagaattg gaaacagata tccattcaga accattttca ctgaattatt caagactggt
    51361 gaatcattta cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac
    51421 ggaaatcgta gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat
    51481 ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt
    51541 ggccatccat atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga
    51601 ggaggaaaaa aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt
    51661 gcggtcttac ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt
    51721 aacgacattg tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct
    51781 ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc
    51841 ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct
    51901 actttattga atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta
    51961 tttattggta ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca
    52021 aaattaccga tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc
    52081 tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct
    52141 ttagtttctt ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc
    52201 ttcttcaacc gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg
    52261 tttgacgtag caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc
    52321 gtaacaggaa tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga
    52381 gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg
    52441 ctagcagcag cttttgcttc attctttact ggtgtgacag aaccacttga attttcattt
    52501 atgttcgttg cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca
    52561 gtgagtgcat tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac
    52621 ttcctatctt tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta
    52681 gtaacagcag tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg
    52741 actcctggcc gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac
    52801 aataaatatg cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta
    52861 acttcgatcg ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc
    52921 gatcaaaata aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat
    52981 gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat
    53041 ggtggtgctg cagcacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc
    53101 ggtgaaacga tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact
    53161 gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca
    53221 gaaaatggag aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa
    53281 catgctgtcg gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac
    53341 actgttgact taaaaggcga accattcact ttatatgtag aagaaggtca aaaagtcgca
    53401 agaggtcaat tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg
    53461 gatatggtcg ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca
    53521 cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc
    53581 ttccccagac ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct
    53641 cctttttccg aataaaaacg gcgttctcct gccaactccg cggtaataag tccagctaaa
    53701 cgaaacatat gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct
    53761 ttttcacaaa atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac
    53821 attattcctg atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca
    53881 atactttttc taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga
    53941 tcgctaaaaa catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa
    54001 acgctgcatc acttgcttca ataatttcta actctttacc tgtcgcacgc tctaatgcct
    54061 tggctaattc ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac
    54121 tttctactag tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt
    54181 ttccatttcc tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta
    54241 attcattctc tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa
    54301 tcattttttc agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg
    54361 caaaacttgt ataagcgata taagataccc cagctgcttg tgctgccttc actacattct
    54421 tatgttcggc ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt
    54481 tcaacgcttc tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag
    54541 cttctttcag tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg
    54601 gaaccatctt ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta
    54661 ctaaataatg catatttgcc actccctatt ctttttattt gttaagattt acagctgtac
    54721 atttataaat tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg
    54781 aaaggatgac aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc
    54841 tatattgaaa tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc
    54901 gaaacaaatg cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc
    54961 tttacaaaaa ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta
    55021 ctagatgtct atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca
    55081 aatccaaatt gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg
    55141 ctgcagaaaa aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa
    55201 atagccgttt tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta
    55261 taattctctc tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat
    55321 tctccggggg acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc
    55381 gctgaagtca tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct
    55441 gaaaaatgca aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag
    55501 cgctactttc acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc
    55561 ccatatattt cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg
    55621 cgtaatcttc tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta
    55681 attcttcagg cgattctgct aaagcgctta caacaagata cgttcctttt gggaatggga
    55741 tgattcgcgt agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt
    55801 tcccgttata gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg
    55861 catctattgt tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac
    55921 cgacaaaatc cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg
    55981 gtttttcttc gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat
    56041 cttacataaa attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat
    56101 tttttaacta tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat
    56161 cctctcccca ttttgtaaaa gagatctcgg gagcttcgac taacccataa tctgaagaag
    56221 gaagccattc tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt
    56281 tattaggaaa aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg
    56341 gattttcttt tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc
    56401 ggtctccatc aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact
    56461 tatgcatctc ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat
    56521 tgactccttc aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt
    56581 tcttttcgat acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc
    56641 gtggaaaaga tttttgtatg ccgtatctac tgatttctga aggcagatat cctgaccaat
    56701 cacgaaaagc tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg
    56761 tcactcttga tccatctaga agatcgcgat tagccaaagc caatctacgg tttttgatat
    56821 agtccattag cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga
    56881 ttcctgtatt ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt
    56941 aatcgatcaa ttggttcaga tctttaagca tatttatctt cctccttgat tgccttttta
    57001 tcatattatc aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag
    57061 aaaatgttgt atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta
    57121 ttatctgcct atctatgttt tacctttaga aaaataaatt ttggggaaga cagattctaa
    57181 gtaaacaaag cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt
    57241 acaaagcaaa ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat
    57301 acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt
    57361 ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat tcttttactt
    57421 tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc gagcaaaagt
    57481 gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat actaaatata
    57541 aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt ttcctgaaaa
    57601 cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt tgaactatac
    57661 aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag tgatcacttg
    57721 ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa
    57781 acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat ccaactactc
    57841 actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac gggagctttt
    57901 aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta agatgccatt
    57961 tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg atcatattat
    58021 caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa agttcatcca
    58081 agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa
    58141 tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac gaacccttct
    58201 tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac ctcatgcaga
    58261 aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg cctcttattc
    58321 aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat tttctaacca
    58381 aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt caatgaaaaa
    58441 tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga ccttgcttta
    58501 aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc aatattagtc
    58561 atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct tcgttgctgg
    58621 atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt gtaagagagg
    58681 ttccgctgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca
    58741 ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct ccatacttaa
    58801 acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa gatttttcgc
    58861 gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt tacgtttcaa
    58921 tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta acgacaatta
    58981 tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag atataaaaac
    59041 tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac ttgcatttgg
    59101 tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt ccctgctggt
    59161 ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca gcccattgct
    59221 ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga gtatgacgat
    59281 tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca cattgctgac
    59341 agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa agttaaactt
    59401 tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt cttctcttgt
    59461 tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg atttcgccat
    59521 cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa acacctctaa
    59581 acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg gactgatttt
    59641 tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat tgggggtgtg
    59701 gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt actttagcaa
    59761 cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta ccgcaaccgt
    59821 tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg ttcagcagtc
    59881 tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct ttggcttcag
    59941 ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc ttggcaggaa
    60001 ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt atggcaaatt
    60061 actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc gtattcttgc
    60121 ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg tttctacgtc
    60181 tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct tgctcaatgt
    60241 actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt ctccttgcgt
    60301 ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga agctgataga
    60361 ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg ttcccacatg
    60421 tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat cgaataatac
    60481 agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta agtcgtgatg
    60541 ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct tcggacttgc
    60601 agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg aaccgacgcc
    60661 cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt ctggagttag
    60721 accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca aggctgcagc
    60781 ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa ctagttggta
    60841 ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat cgccgacaga
    60901 aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa aacctaagat
    60961 acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag gattaacaga
    61021 tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct gttctttttc
    61081 atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga ctaccttcat
    61141 taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag catccataca
    61201 gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc atcataatta
    61261 ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga
    61321 cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc acaagtcaaa
    61381 atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc agagttaaac
    61441 ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg gtattagctc
    61501 aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac ttgatacagg
    61561 acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg ttcactgaaa
    61621 agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc agtagatttc
    61681 gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat atagatttta
    61741 aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg atcttgcaga
    61801 tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct cgggtctttt
    61861 tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc aacgccatat
    61921 ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat cgtgattagt
    61981 gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc ccaatgtgca
    62041 tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc tatatcttga
    62101 tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc gatacgcgtc
    62161 ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc cgctacttct
    62221 ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac tgggcagccc
    62281 atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat ttctctaaaa
    62341 aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc tgtaaatgtt
    62401 aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga gtttgtaaac
    62461 tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa tacaacatct
    62521 gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt ccaaaaatta
    62581 gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat ctttctcaaa
    62641 aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt aaaagatgtt
    62701 tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt ttccggaata
    62761 aatggtgttc aaaagcttgc atttgcggta ataagtccaa ccaaacgaaa aataggagga
    62821 gctatttttg tttggctgtt cttattactt gatgcagaac agctttttca caacctctcc
    62881 actaaacggt gttcaatcag ctgaccctcg gtattcgctc aaaaaacaac aaaatatgag
    62941 gaactatttt cccgtttgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct
    63001 tttctaactt ctcaatcctc ttcctttatt gatcaaatac caagtaacgg cgtacaaaac
    63061 aacgatgaat aacacaagta cgatcatcga tgttgctact ggcacatcga tcgttccaag
    63121 gaatccgtaa cggaaaccgg agatcatata aacgatcgga ttgacttttg aaacagcttg
    63181 ccaaaagggc ggtaacatag agatggcata gaaaacgcca cctagataag ttaacggctg
    63241 caagacaaat gtaggcacga tcgatacatc atcataagac tgagcaaaaa tcccgttgag
    63301 cagtcctgct aatgaaaaca agattgccgt catcaataat gtaatgatga ccatgaacca
    63361 tgaatacaca tgaagtggta caaagaacaa ggaaatgatc gttacgagag agccgacgag
    63421 aatactgcga cccacaccac caatcacaaa gccccaaata atcacatgcg taggaacagg
    63481 tgctaccaag atttcttcga tatttttctg aaatttttga gagaagaagg acgaagaaac
    63541 attggcatac gaactagtga tagcagacat cataatcaaa ccaggaacga tgaattccat
    63601 atatgaaaaa ccacccatgt caccgatccg cccaccgatc atttttccga aaataacaaa
    63661 atacaaggat gtcgtgatca ctggcggaac cagtgtttgg acccatatac gcaaatagcg
    63721 gttcgtttcc ttgatagcta aactttttaa tgcagtaaaa taaagattaa acatttttct
    63781 cctccgtatg attattttct tctgtgattt tcaagaacag ttcttctaaa cgattcgact
    63841 tattcctcat agaaagaact ttgattcctt gtcttgtaag ctgatcgaag attccgttca
    63901 ccccttgatt tcgttcaact tcgacagcga ttgtctgctc atcttcaaat gtacaagcat
    63961 atccttcaat cacaggtttg gtatcatatg gcgctaaatc aaagataaat gtttcaaatt
    64021 gcagttttga cagaagctct ttcatacttg tattctcgat cagttcaccc gattggatga
    64081 ttccgatgtt gcgacaaagc atttcagctt cttctagata atgcgtcgtc aaaatgatcg
    64141 ttgtaccatt ttcgttcaat tctcgtagaa actcccacat ctcgcgtctc aactcgatgt
    64201 caactcctgc tgttggttca tccaagatca gcaaacgtgg ttcatgcatc aatgccctag
    64261 caatcatcag tcttcgcttc attcccccgg aaagcatacg tgcacggaca tttctctttt
    64321 cccaaagatt ggactgcttc aaatattttt cgctgcgttt catcgcttct ttgcgagaga
    64381 caccgtagta gcctgcttga ttcacgacga tttgctggac cgtttcaaaa gggttaaaat
    64441 tgaactcttg tggaaccaat ccaatctgtt gctttgctcg aactagatcc ttgtccagat
    64501 cataaccgaa gacattcact tttccagatg ttttattgac taaagaagta ataatcccga
    64561 tcgttgtaga cttcccagct ccatttggcc ctaatagcgc atagaaatct ccttctttca
    64621 ccgaaagatc gattccccgc aatgcttcga ccccacttcc atagatcttt ttcaaattat
    64681 ttatttcaag cgcatttgcc atttatttcc tatctcctta tagacatgat tcttaattgt
    64741 atcatggaat acagcagatg aaaaaacaac ttggcttcca cacaaaaaca cacctgctaa
    64801 caaagaggag atgtgttttc tagttcatga tcatcttcga tagaataact atctaaacaa
    64861 ctctggaact tttaaaatca taaaagtacc gacagcaact actacgatga catatagaag
    64921 atccttataa ctgaggttct tgtctttttt attcatgtta agctttcctc cgtcctttta
    64981 tcgaaagctt ttttctttta ttttaaccga aataaataaa atggaaatag ttattttcaa
    65041 tttctgtttt cgctgtttgt tttacggaga actttgccat ggaacgtgcg tctcgtcgct
    65101 gatgctaagt tttttcgata aaagctgcta gaaggaagac agcgtggttt attttgtatc
    65161 gtcttttgct aaatcttcca agatcatctg gacagcagac tcgttcccat tgaattctgc
    65221 cacttttttg gcatcaatgt acttcttttg ataatcgtct ttttggaccc acgcatcatg
    65281 cttcgtttga taaagttaat caaacggata ccactgacgc tgttcatcga acaacaaact
    65341 ttctttttgg gcctgttcaa ctatggattt aggataattc aaaactttca gcaaggcaat
    65401 cgcatttcgg gtccttgaag gtccttcttt taatttgaaa tcaaaactga ttccttttcc
    65461 ttctgttact tgttcttcaa agtggacatt ctcacaataa ttctttaaaa tctctgtcaa
    65521 ttcgatatca tgtgtggcga caaatgctaa agatggatac tccgcaagcc accgaacgac
    65581 tgaagcggaa gaagcaatcc gttcgatcgt atttgttcct tttaaaatct catctacgaa
    65641 gcagtagcag cgttctttgg aagccacttg gtccagcaaa cgtttgatcg atttgatttc
    65701 tgaaacaaaa taactgtcgc cttcaaataa atcatcttcg attgccatgg aagtcaacac
    65761 atgtccaggt tgcagggtaa actgttctgc aatcgctgtt tggatcgtct gtgctagaat
    65821 acagctgatg gctatacttt tgacatacgt agattttcct gatgcattgg aacccgtgac
    65881 taatgtattc ttctgccagt tgactggatt aactaccgct tcttttaaca atggatgata
    65941 gatatctttc gctgtcacac cgccctcagt aaactctggt cggcaagtga tcggcatata
    66001 ggtgcgataa ttcaaaacgg ctgctgcaac ttccaattct cccagcaatt cccacagctt
    66061 gatggcttca gttgaatgct tttctaattt agtgagaacg aaatgatagg agataaatgg
    66121 cagcatgaac atgatgttga catattcaaa aatcaattct gcttcactcc cgctcttcat
    66181 tcgaaatgaa aaacctaatt tagggatgtc tcttaatggt ttagtttgct gtttcagctc
    66241 tttttgaagt ggtgtcgtca tttttgctaa ctgattggcg gctgaaatcg ttgaaaccaa
    66301 ataactcatg ctattcagct ctgtttctag tgtttgtttc ttcactagat aatagaccgt
    66361 attgaatact aagccaccga ttgctagata gattcctagt acttgcccta aaagaagcaa
    66421 acctacaccc accagcggaa gcaagccaag aaaaataaat aaacctacgc gtccaatctt
    66481 tttacttttt ccatccgcta gatattgttt tgcaaaatta tgatcttttt ttcctaaacg
    66541 agcgaattga tattggattc tttctctcag ttgaggattt tcctgataaa aagcaattag
    66601 tttttctaac tgataatctt ctccaaaatc aaagctacgt aaacgctgat acaacgcttc
    66661 tgaacctaca ctagaatagg tactgttgat cccttcaaat acttcgaaca tatccaagtc
    66721 ataccaagta agatcgtcga tttcactatc ccatgaacgg aattttttct ccgtgagcca
    66781 cgcatccttc aaactttctt ctttatcaaa tctcggttga taaggaattt ttccccaatt
    66841 actacgaact gtttccttta ctttcaaacg tgaaaaaatg tctaaaacaa ctaataaaat
    66901 aacaactgca ataataatta aaacgataaa ttctctttgg cccatatccc tctccgttcc
    66961 ttgatttgtt agttttagta tagcactgtt gcgctatttg aaaagggggt acaaaaataa
    67021 ctgtcttcgt tacctaattc gtatcaatca caccaaaata aaaacacctt ccaaaaaaga
    67081 agatgtcttt aaaacgagat acaatttttt gtcattcact tcaggtaaat tagagccaat
    67141 taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct agcggtcatc
    67201 tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc gctagattcc
    67261 gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc aacagaggtt
    67321 tgcgctcctg gtttgatagg attggccact gttaatcgaa tgaatgaatc ctcttttgaa
    67381 aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat attttgcagc
    67441 actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg ttcagcgaac
    67501 gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac ttcttccacc
    67561 tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc tattcgagca
    67621 agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt gatgatcgtt
    67681 aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac ataaccgtta
    67741 atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag tctgatgttt
    67801 tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaccca tgtattcagt
    67861 gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg accaccatat
    67921 tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc cctttgcagg
    67981 aagaaaataa aacttcccat aaagataaca acgagaagca acagccactt aatcaaatct
    68041 aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta gtttccctcg
    68101 aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt cttgccaaac
    68161 tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa aaagcttcag
    68221 cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta catccctcga
    68281 ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt ttacttggac
    68341 agaagtctga tgacgaagtt ggatgccgat tcgtaacaac agttcttcga tatcaaatgg
    68401 tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc tttgatcgct
    68461 ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg ctaacaatgc
    68521 ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag gatatgtgct
    68581 taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc tctgttcaag
    68641 aacctcctcc agtaaagaat gaacagcttc atcgtcttcg ataatcaaaa tagtttccag
    68701 caggaacacc tacttaatgt cagatttttg gaaaataaac attcctagag ctgatgatac
    68761 cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag ttgcttgttg
    68821 cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa attccaagcc
    68881 tttgattttt gcgaaaacac cgggaacgaa ggccgttccc atgatataaa gaaaagaaag
    68941 aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa cagataatgt
    69001 cgctaatatc agcagaagca atacaggaaa tacttttaga acataatttc catagttttt
    69061 gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaatcatgg ctagccccgt
    69121 aatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg acacataata
    69181 agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt gaaattcttc
    69241 cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag ccattttttc
    69301 caccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt ttaattcatt
    69361 gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa gtacgaccat
    69421 cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt cagcagttaa
    69481 tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc agtttttgcc
    69541 caactaggtg gataccatcc acaggaactt ttgcctctac caatgcttga ttgatggctg
    69601 ctacttggtc aattccttca aaaagctgga taacacggct agattggaca aaataatccc
    69661 tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg tcaattttta
    69721 taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc aagtgtcctt
    69781 catgcaaaat accgtagtga gtggcgatct gtgacaattc atctagcaag tggctagaaa
    69841 gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc atttctgcca
    69901 tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct ggattcgtga
    69961 tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc tttgctttct
    70021 ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct ctatccgaca
    70081 caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct ggataaagag
    70141 caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc gtcgttccaa
    70201 ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc ataaaggtag
    70261 acttacccgc tccattctcc ccaatcaaac catagatcat tccacgtttg atttcgatcg
    70321 atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt gctttcaaga
    70381 ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgataagtg aagtatagtt
    70441 gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt ccaattgaat
    70501 ttcaagaaaa atcagactgt ctcgcagacg tggttgagtt gtctgccaat caaagagcgc
    70561 accaaacagt gctaatccaa gtggactggc caccatggta atcgttgtta tcgttgacat
    70621 cagacgtcct tgtatatgcg ttggaacttc tcgctgaagc cagaccatat aaaaagcaaa
    70681 agaaaacatt aatccttaaa aacggactta tgaaaaataa tacatgccgt ttgggattta
    70741 caattagcta ccaaataaga ggttatgaca aaagactttt cacaatccct tcttccgaat
    70801 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatttatca aacatgagaa
    70861 gcaatttttg taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaga
    70921 agcggataaa taatattgtt tgatgatact ttatcttttt cccgtttgtt tatagatcga
    70981 atgatattat agaccaagaa tcctacctat taacatagct aaaaacacaa aaaaggcact
    71041 ttccaaattg tggaaagtgc cagaaaagtt aggattaagg taactgcgct cagcattgct
    71101 tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat aatcgcaatt aacgttttga
    71161 gaactgtgaa gctttacggg ctttcttaag acctggttaa acaaaactct attttattgc
    71221 attttacagg aatgtatttc atgcgaaatt aataagtctt tgtttataga gttttactag
    71281 ggtataaaaa gttgtatgtt atcattacct caaaacatta cctcagcttg gcgagttggt
    71341 tgattgaaga ataaatcatt tatctagata tctcaataaa aaatgatttc taagaaactt
    71401 gtattttcgt gttattattt ttttgcccta tcaaaatttc taattcttct agcgtaatat
    71461 gattatgaca cataatcgat tcaattactt caatacctat ttttttcgag aaagatccct
    71521 tttgttcact cacaaaaata ctttcagaag acatatataa ggaacgaagt tttggaatta
    71581 gtatatcgta caaatagacc accataaaat cgtccttgca accattctca aaaagaatta
    71641 aatttgctga ttgtgttaca tatatcactg aattacattc atggtcaatt ccaaaaacaa
    71701 tgtctacttc attttctttt ctaattcgcg cagttacatt tttcaaactt tcaccattac
    71761 ttataaatgc tttagttact gtaagctctg atgtttgttc agatattcct tttataatat
    71821 tttgttcatc agccacattc cccataaatc cgccgattcc ttttaagtaa taattctcac
    71881 ctaagagtcg cttgcattca ctgaattgat aaaaaaacca gttaaaaaaa tcttctggaa
    71941 aaataaattc tgatcgaact ttatttatat ttttagctcc tattaatttt ataactcgat
    72001 taatgttcac ttgctgcgat gaacatataa ctgaataaat tgagttgttt aattcaaaaa
    72061 aacaacttta acatatgcat cattaattcg ttatttttta ggtagatatt gatcattgtt
    72121 atctctcttt ttttgcttcc catactccaa cttagaaatt gccgttataa aatttatttt
    72181 aaaggtgact ttttcccctg aagtatcggt agtaggaata cttaatacct cagcttctga
    72241 ggaggtatcc caattatagt tatactttgc atcatcacca taaagaacaa ttccattttt
    72301 tttatcttgt gccgttagct ttactggttc gcttactttt cttgttaata tcttggactt
    72361 tacatcatcg atattgctta ttttatcaag cttaaataat ttacaaatta atttcgtact
    72421 agtttttata tttg
//
LOCUS       DACDNO010000007        62842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDNO010000007 DACDNO010000000
VERSION     DACDNO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.007"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWI22_000486"
     CDS             591..1280
                     /locus_tag="IWI22_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9167905.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWI22_000487"
     CDS             1267..2475
                     /locus_tag="IWI22_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9167906.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWI22_000488"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWI22_000488"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9167907.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWI22_000489"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWI22_000489"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9167908.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWI22_000490"
     CDS             complement(6578..7273)
                     /locus_tag="IWI22_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9167909.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWI22_000491"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWI22_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9167910.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWI22_000492"
     CDS             complement(8472..8663)
                     /locus_tag="IWI22_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9167911.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWI22_000493"
     CDS             complement(8684..9574)
                     /locus_tag="IWI22_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9167912.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWI22_000494"
     CDS             complement(9561..10328)
                     /locus_tag="IWI22_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9167913.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWI22_000495"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWI22_000495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9167914.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWI22_000496"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWI22_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9167915.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWI22_000497"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWI22_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9167916.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWI22_000498"
     CDS             14944..15282
                     /locus_tag="IWI22_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167917.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWI22_000499"
     CDS             complement(15450..16208)
                     /locus_tag="IWI22_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9167918.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWI22_000500"
     CDS             complement(16226..17044)
                     /locus_tag="IWI22_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9167919.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWI22_000501"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWI22_000501"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9167920.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWI22_000502"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWI22_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9167921.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWI22_000503"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWI22_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9167922.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWI22_000504"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWI22_000504"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9167923.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWI22_000505"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWI22_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9167924.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWI22_000506"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWI22_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9167925.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWI22_000507"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWI22_000507"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9167926.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWI22_000508"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWI22_000508"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9167927.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWI22_000509"
     CDS             27086..27385
                     /locus_tag="IWI22_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9167928.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWI22_000510"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWI22_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9167929.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWI22_000511"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWI22_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9167930.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IWI22_000512"
     CDS             28352..30325
                     /locus_tag="IWI22_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9167931.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IWI22_000513"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IWI22_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9167932.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IWI22_000514"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IWI22_000514"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9167933.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IWI22_000515"
     CDS             33204..34496
                     /locus_tag="IWI22_000515"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9167934.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="IWI22_000516"
     CDS             35232..36095
                     /locus_tag="IWI22_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9167935.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="IWI22_000517"
     CDS             complement(36224..37846)
                     /locus_tag="IWI22_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9167936.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="IWI22_000518"
     CDS             38095..38616
                     /locus_tag="IWI22_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167937.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IWI22_000519"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IWI22_000519"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9167938.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="IWI22_000520"
     CDS             40697..41515
                     /locus_tag="IWI22_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9167939.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="IWI22_000521"
     CDS             complement(41618..41815)
                     /locus_tag="IWI22_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167940.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="IWI22_000522"
     CDS             41984..42562
                     /locus_tag="IWI22_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9167941.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43607..44110)
                     /locus_tag="IWI22_000523"
     CDS             complement(43607..44110)
                     /locus_tag="IWI22_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9167942.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44469..45494
                     /locus_tag="IWI22_000524"
     CDS             44469..45494
                     /locus_tag="IWI22_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9167943.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45817..46695
                     /locus_tag="IWI22_000525"
     CDS             45817..46695
                     /locus_tag="IWI22_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167944.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47171..47287
                     /locus_tag="IWI22_000526"
     CDS             47171..47287
                     /locus_tag="IWI22_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9167945.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48215..48553
                     /locus_tag="IWI22_000527"
     CDS             48215..48553
                     /locus_tag="IWI22_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167946.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48647..48877
                     /locus_tag="IWI22_000528"
     CDS             48647..48877
                     /locus_tag="IWI22_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167947.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49277..49648
                     /locus_tag="IWI22_000529"
     CDS             49277..49648
                     /locus_tag="IWI22_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9167948.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49922..50614)
                     /locus_tag="IWI22_000530"
     CDS             complement(49922..50614)
                     /locus_tag="IWI22_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9167949.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50617..51402)
                     /locus_tag="IWI22_000531"
     CDS             complement(50617..51402)
                     /locus_tag="IWI22_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9167950.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51639..53039
                     /gene="lacG"
                     /locus_tag="IWI22_000532"
     CDS             51639..53039
                     /gene="lacG"
                     /locus_tag="IWI22_000532"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9167951.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53064..53381
                     /locus_tag="IWI22_000533"
     CDS             53064..53381
                     /locus_tag="IWI22_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9167952.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53394..55067
                     /locus_tag="IWI22_000534"
     CDS             53394..55067
                     /locus_tag="IWI22_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9167953.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55081..56712
                     /locus_tag="IWI22_000535"
     CDS             55081..56712
                     /locus_tag="IWI22_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9167954.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57584..58774
                     /locus_tag="IWI22_000536"
     CDS             57584..58774
                     /locus_tag="IWI22_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9167955.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            58908..61097
                     /locus_tag="IWI22_000537"
     CDS             58908..61097
                     /locus_tag="IWI22_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9167956.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            61094..>62842
                     /locus_tag="IWI22_000538"
     CDS             61094..>62842
                     /locus_tag="IWI22_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9167957.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLY"
BASE COUNT        19618 a        11379 c        12395 g        19450 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgttttcc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
    43321 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
    43381 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
    43441 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
    43501 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
    43561 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
    43621 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
    43681 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
    43741 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
    43801 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
    43861 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
    43921 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
    43981 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
    44041 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
    44101 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
    44161 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
    44221 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
    44281 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
    44341 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
    44401 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
    44461 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
    44521 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
    44581 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
    44641 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
    44701 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
    44761 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
    44821 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
    44881 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
    44941 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
    45001 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
    45061 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
    45121 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
    45181 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
    45241 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
    45301 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
    45361 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
    45421 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
    45481 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
    45541 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
    45601 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
    45661 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
    45721 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
    45781 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
    45841 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
    45901 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
    45961 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
    46021 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
    46081 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
    46141 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
    46201 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
    46261 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
    46321 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
    46381 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
    46441 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
    46501 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
    46561 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
    46621 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
    46681 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
    46741 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    46801 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
    46861 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
    46921 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
    46981 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
    47041 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
    47101 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
    47161 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
    47221 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
    47281 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
    47341 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
    47401 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
    47461 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
    47521 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
    47581 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
    47641 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
    47701 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
    47761 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
    47821 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
    47881 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
    47941 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
    48001 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
    48061 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
    48121 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
    48181 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
    48241 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
    48301 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
    48361 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
    48421 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
    48481 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
    48541 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
    48601 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
    48661 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
    48721 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
    48781 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
    48841 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
    48901 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa
    48961 tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct
    49021 acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta
    49081 gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa
    49141 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
    49201 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
    49261 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
    49321 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
    49381 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
    49441 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
    49501 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
    49561 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
    49621 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
    49681 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
    49741 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
    49801 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
    49861 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
    49921 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
    49981 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
    50041 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
    50101 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
    50161 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
    50221 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
    50281 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
    50341 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
    50401 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
    50461 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
    50521 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
    50581 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
    50641 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
    50701 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
    50761 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
    50821 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
    50881 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
    50941 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
    51001 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
    51061 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
    51121 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
    51181 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
    51241 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
    51301 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
    51361 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
    51421 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
    51481 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
    51541 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
    51601 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
    51661 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
    51721 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
    51781 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
    51841 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
    51901 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
    51961 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
    52021 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
    52081 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
    52141 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
    52201 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
    52261 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
    52321 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
    52381 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
    52441 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
    52501 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
    52561 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
    52621 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
    52681 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
    52741 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
    52801 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
    52861 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
    52921 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
    52981 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
    53041 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
    53101 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
    53161 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
    53221 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
    53281 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
    53341 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
    53401 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
    53461 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
    53521 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
    53581 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
    53641 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
    53701 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
    53761 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
    53821 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
    53881 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
    53941 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
    54001 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
    54061 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
    54121 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
    54181 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
    54241 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
    54301 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
    54361 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
    54421 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
    54481 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
    54541 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
    54601 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
    54661 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
    54721 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
    54781 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
    54841 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
    54901 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
    54961 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
    55021 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
    55081 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
    55141 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
    55201 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
    55261 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
    55321 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
    55381 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
    55441 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
    55501 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
    55561 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
    55621 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
    55681 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
    55741 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
    55801 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
    55861 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
    55921 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
    55981 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
    56041 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
    56101 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
    56161 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
    56221 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
    56281 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
    56341 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
    56401 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
    56461 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
    56521 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
    56581 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
    56641 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
    56701 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
    56761 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
    56821 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
    56881 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
    56941 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
    57001 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
    57061 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
    57121 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
    57181 ccctccatat tatgttggct ctttattccc actctgtttt caacgtaaag tgtataaaac
    57241 agaatgcgat actatcaagg gagttaaaac aatatacaga acagtaaaac actataaata
    57301 aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat catactctgt ttatatatga
    57361 aaactagatt ctgaatccaa tatttggtta acgagaggcg ttcgaatttg aacgtctttt
    57421 atatattact ttcaaataaa tatgaggtag aatactatct attttctttt taacccaatt
    57481 gttataatgg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc ccttctgtag
    57541 aatagagtta ccacaacaca ttcacagaag agaggacttc cctatgaacg attttactac
    57601 agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc gtgtccattt
    57661 ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag attacgaaaa
    57721 gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg accgtacggt
    57781 caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa
    57841 gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga ccgtcattca
    57901 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
    57961 gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag aagaggtaac
    58021 ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg acgcaacgta
    58081 tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag ttggcattcg
    58141 cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat ccatcacgat
    58201 ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat
    58261 cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca aagctcgttt
    58321 tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg
    58381 taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt
    58441 ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc
    58501 gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca tacgcaagag
    58561 tatttattct acgaacttga ttgaatcctt taataaacaa atcaagaaat acagccaccg
    58621 caaggaacag ttccaaaatg aagagtcgat ggaacgtttc ttagtctcgt cttttgatac
    58681 ttacaaccaa aaattcctag gtcgcagtca taaaggtttc caacaggccg aaggcgagct
    58741 cgaacaaatg ctaagccaac tgattgagaa ttagaaacaa tctgcagtag ggaagaatca
    58801 tttacacaaa attattgacg ctcccttata atgagaactt ttactaacat aatttatgat
    58861 aaacttacta tataatcata ttaattaaat agttaggaga tctgcttatg tttatatcta
    58921 ctcttcaaat tcgaaatttt agaaattatg aatccgaaat ctttcgattc gataatgaga
    58981 caaatactat tataggtgag aatgattcag ggaaaaccaa tgctcttact gcattgagaa
    59041 tcttattaga tgatagttat tactatagta gtaaaacact aaaagaatca gatttttttc
    59101 atggaattca aaatgggtgg caagggcatt ggattattat ctctgcgaca tttgaaggaa
    59161 tcagcgaaga agaatttgac aatgagattt gtgcttccat ttctttaaat tctgaaagtc
    59221 aaacaatctt agaagaattg atttcaaatg cagataaagg tgtaggtagt atttctctat
    59281 ttattagacc taacaaagct attagaaagc aattatttga tatttccact gaattagact
    59341 ctcatcaatt taacgaattt agaaattcta ttcgtctatc cgactatgaa ttttactaca
    59401 catctaaatc aaatctaaat ttctgtatca atgaaaatta tgataaattg gtaggaagac
    59461 tcaatgacaa tatagctagt aatcctgaag aagacgatga agctctgttg ggctcaagaa
    59521 ttgacatgtc agatattttc aagcatattt cggttgttta tgttgacgct cttcgagatg
    59581 ttcttagaga aatgaaaaac aatcgaaacc cagtcaaaag aatcatggaa acaattgaat
    59641 caaaaatatc atcagataat gttgagtcat taaaaacaat tatacagcaa ttaaatgaga
    59701 ctattaccag tgttcctgaa attaggaaaa ttggcgaaaa tattaatcgg caactaaata
    59761 gcataattgg ttcagtgtat tctcctaatc ttcttctttc ctcaactatg tcagatgata
    59821 tgggttcttt agcaaaattt ctttcaatga agccagaaca aaatatcgac ttagacttac
    59881 tagggttagg acatctaaat atgatttatt tagctttgaa aatagtcgaa tttgaagcgt
    59941 gtcgttcaag agaactactt aatattatgt tgattgaaga accagaagct catattcatc
    60001 atcatattca aaaaactctg ttcgaaggat taaatcttca aaaaaactat actcagattc
    60061 tcatgaccac acattctgta catttagctg aagtgtctga aatatctcgg atgaatgtat
    60121 taaaatcgtt taatggcaaa tctatcgtta tgaatccagc aaataatctg aactcatttg
    60181 ctaaagaaaa gttaaaaaag aacaatctaa atctaataaa cgcagtagag agatatttag
    60241 atgttaaaag aaatggattg cttttttcta aaggtgttat cttagttgaa ggagatgctg
    60301 aagaaatact tattcctcaa atagtaaaaa aattatttgg tataaactta gacgaacttg
    60361 gaataggttt gattaatgtt ggtagtactt cgtttgaata tatcgctagc ttgtttgacg
    60421 atgatagaat acagagaaag tgtgctattg taactgattt agatgaacag gcaatcccat
    60481 cagaacatcg attgtataaa agcactgcac aagagaaagg tcaagcaaga aaagaaaaat
    60541 tatcctccct ttttaatgaa aacaattggg ttaattgttt ttatgctaat acaacatttg
    60601 aaattgagtt tttagatgtg aataaagaaa atattctaac ttatgtcagt ccaattatag
    60661 atgacgcatt caaagataaa aaaacaataa ctgcttataa agaagaaata caaagtgaac
    60721 aatatttaga aagaaattta gcgatgttaa aactagccaa tcatgttggt aaggggtggt
    60781 ttgcaattga gttaggtgaa aaaattgact ttttaatcaa aattcctaag tatattctta
    60841 atgctattgc ttttgcatgt caggaaatag tagatctctc aatttataaa aaaattctac
    60901 tccatacaat cgacttgtac gaggaaacta cagaattgtc agctttatca gctagtttaa
    60961 aaacaagtga tacagctgat ttgaatatag caataatttc tttattagag tactttggta
    61021 aagatgagga tgttgtgtat ctaatcaatc agatagaaaa ttacacaaaa atactttatg
    61081 ctgaggtttc aaaatgacaa atatcttcga aaaaatcgta aatgatttaa atttagaaca
    61141 aaaagatgtt gtggaaagtg aagataacct ttatgtaaat gcatgtcctg ggagtgggaa
    61201 aacaagggta cttacaagga aaattgctta ccaagcaatt aaacacgatg gatcactaaa
    61261 aagaataatt gctatcacat acactaatag agctgctgaa gaaattaagg aacgcctaag
    61321 tttacttagt atagatgatg atattaatat ttgggtgggg acaatacatc agttttgtct
    61381 tgaatttatt atttatcctt ttaaaatgaa tctctcaaga gtatcaaaag gatttacaat
    61441 tattgatgat tatactcaaa gagcttacgt cagccaaatt aataatatgc tgaatttaaa
    61501 tctcaaaacg tatgaactga acaaaattga tttgtcactt tctcatgatt ttaaaattaa
    61561 tgaagttcag tatactcaag tttctgaaat gtatcacact atgttggaaa aaaataaaga
    61621 aattgatttt gatatgattt tactagtagc gtataaaatt ttaactgtaa acagtaacgt
    61681 tgcacgtaat attgctagta atataagggc gatttatgtg gatgaatttc aagatactag
    61741 agaattacaa tacaatgtaa tagctaaatt acttcaaaac aaccctcaaa ttcagtcaat
    61801 gtttgttgga gatattgacc aagcaattta tggatcatta gatggtatag ctaaatcagt
    61861 tgaagaacta gaaatgctaa ctagtcacac ttttcaatca aagacgctac atggctgtta
    61921 caggtctaat cagagattag tagacttcta ctctaatttt cagagttgtc cttacgagat
    61981 tgaaagcaga gggaataata aaaacgaccg tggttttata agttatgatt gcaaagttac
    62041 taaggaggat ttacccaaca taataaaaaa tataataaag gaaaagattg aaagcggcat
    62101 tcatgaaaaa gatatttgtg tacttgctcc tcaacactat cctctgtttt cactgggtga
    62161 gtatttaaag aaagaattac cgtattgcaa ttttgatgct ataaatatat caccaattaa
    62221 ggtaaataat catagtttat ttttcaagtt agcaattttg tactttactg aatcaggtga
    62281 aaaggttcga agaagaaagc taattgcaaa cgatatttta attatattaa aaaatgaatt
    62341 tggaatagaa gttaaagatt attttattga cttagattta ttacagctaa taaacagtgt
    62401 gaaaagaatg tatagtggag acgacaacgg tgttgattta tacaaatttg ttaccaaaca
    62461 tatatttgtt aatcttaata ttgacgagaa aaattacccg tctattttta agcactatct
    62521 attttttatt aacgaagtag aagatcggat aaaaaataat tcattatcta gtagtgtatc
    62581 tagttttaag aagatgttta aatctcgaga tggtatcaca cttactacag cacataaagt
    62641 taaaggaaat gagtatgata ctgttatcgc tattaatctt ctaaacggga aaattcctca
    62701 ttggaatgaa atttttaatg tttctgataa aggaattagt tcagcgagaa aactactata
    62761 cgtaatttgt tcaagagcaa aaaataattt atttcttttc tcagaggtgg gttattttac
    62821 gagtaaagga gacttgcttt at
//
LOCUS       DACDNO010000008        62329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDNO010000008 DACDNO010000000
VERSION     DACDNO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.008"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..251)
                     /locus_tag="IWI22_000539"
     CDS             complement(21..251)
                     /locus_tag="IWI22_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167958.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IWI22_000540"
     CDS             complement(687..1253)
                     /locus_tag="IWI22_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9167959.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IWI22_000541"
     CDS             complement(1253..2209)
                     /locus_tag="IWI22_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9167960.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IWI22_000542"
     CDS             complement(2209..2865)
                     /locus_tag="IWI22_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9167961.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IWI22_000543"
     CDS             3235..5253
                     /locus_tag="IWI22_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9167962.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IWI22_000544"
     CDS             5680..8094
                     /locus_tag="IWI22_000544"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9167963.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IWI22_000545"
     CDS             8442..9995
                     /locus_tag="IWI22_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9167964.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IWI22_000546"
     CDS             complement(10376..12160)
                     /locus_tag="IWI22_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167965.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IWI22_000547"
     CDS             complement(12175..13896)
                     /locus_tag="IWI22_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167966.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IWI22_000548"
     CDS             13996..14757
                     /locus_tag="IWI22_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9167967.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IWI22_000549"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IWI22_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..20065
                     /locus_tag="IWI22_000550"
     CDS             16664..20065
                     /locus_tag="IWI22_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9167968.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(20489..20659)
                     /locus_tag="IWI22_000551"
     CDS             complement(20489..20659)
                     /locus_tag="IWI22_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9167969.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(20853..21032)
                     /locus_tag="IWI22_000552"
     CDS             complement(20853..21032)
                     /locus_tag="IWI22_000552"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167970.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            21419..22531
                     /locus_tag="IWI22_000553"
     CDS             21419..22531
                     /locus_tag="IWI22_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9167971.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(22609..22902)
                     /locus_tag="IWI22_000554"
     CDS             complement(22609..22902)
                     /locus_tag="IWI22_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9167972.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            23070..23384
                     /locus_tag="IWI22_000555"
     CDS             23070..23384
                     /locus_tag="IWI22_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9167973.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            23405..25384
                     /locus_tag="IWI22_000556"
     CDS             23405..25384
                     /locus_tag="IWI22_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9167974.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            25397..25723
                     /locus_tag="IWI22_000557"
     CDS             25397..25723
                     /locus_tag="IWI22_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9167975.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            25739..26257
                     /locus_tag="IWI22_000558"
     CDS             25739..26257
                     /locus_tag="IWI22_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9167976.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            26257..27609
                     /gene="celB"
                     /locus_tag="IWI22_000559"
     CDS             26257..27609
                     /gene="celB"
                     /locus_tag="IWI22_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9167977.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(27693..29198)
                     /locus_tag="IWI22_000560"
     CDS             complement(27693..29198)
                     /locus_tag="IWI22_000560"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9167978.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(29290..29880)
                     /locus_tag="IWI22_000561"
     CDS             complement(29290..29880)
                     /locus_tag="IWI22_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9167979.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            30059..31705
                     /locus_tag="IWI22_000562"
     CDS             30059..31705
                     /locus_tag="IWI22_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9167980.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            31754..32473
                     /locus_tag="IWI22_000563"
     CDS             31754..32473
                     /locus_tag="IWI22_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9167981.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            32952..33899
                     /locus_tag="IWI22_000564"
     CDS             32952..33899
                     /locus_tag="IWI22_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9167982.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            33911..34831
                     /locus_tag="IWI22_000565"
     CDS             33911..34831
                     /locus_tag="IWI22_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9167983.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            34872..36329
                     /locus_tag="IWI22_000566"
     CDS             34872..36329
                     /locus_tag="IWI22_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9167984.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            36674..37288
                     /locus_tag="IWI22_000567"
     CDS             36674..37288
                     /locus_tag="IWI22_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9167985.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            37281..39005
                     /locus_tag="IWI22_000568"
     CDS             37281..39005
                     /locus_tag="IWI22_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9167986.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            39002..40453
                     /locus_tag="IWI22_000569"
     CDS             39002..40453
                     /locus_tag="IWI22_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9167987.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(40926..43079)
                     /locus_tag="IWI22_000570"
     CDS             complement(40926..43079)
                     /locus_tag="IWI22_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9167988.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(43081..44127)
                     /locus_tag="IWI22_000571"
     CDS             complement(43081..44127)
                     /locus_tag="IWI22_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9167989.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            44277..45566
                     /locus_tag="IWI22_000572"
     CDS             44277..45566
                     /locus_tag="IWI22_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9167990.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            45579..48281
                     /locus_tag="IWI22_000573"
     CDS             45579..48281
                     /locus_tag="IWI22_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9167991.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            48266..49123
                     /locus_tag="IWI22_000574"
     CDS             48266..49123
                     /locus_tag="IWI22_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9167992.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(49194..50051)
                     /locus_tag="IWI22_000575"
     CDS             complement(49194..50051)
                     /locus_tag="IWI22_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9167993.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            50262..52433
                     /locus_tag="IWI22_000576"
     CDS             50262..52433
                     /locus_tag="IWI22_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9167994.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            52501..52842
                     /locus_tag="IWI22_000577"
     CDS             52501..52842
                     /locus_tag="IWI22_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9167995.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(53118..54872)
                     /locus_tag="IWI22_000578"
     CDS             complement(53118..54872)
                     /locus_tag="IWI22_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9167996.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(54883..55719)
                     /locus_tag="IWI22_000579"
     CDS             complement(54883..55719)
                     /locus_tag="IWI22_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9167997.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(55721..56404)
                     /locus_tag="IWI22_000580"
     CDS             complement(55721..56404)
                     /locus_tag="IWI22_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9167998.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(56563..57957)
                     /gene="uxaC"
                     /locus_tag="IWI22_000581"
     CDS             complement(56563..57957)
                     /gene="uxaC"
                     /locus_tag="IWI22_000581"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9167999.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(57996..59495)
                     /locus_tag="IWI22_000582"
     CDS             complement(57996..59495)
                     /locus_tag="IWI22_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9168000.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(59488..60909)
                     /locus_tag="IWI22_000583"
     CDS             complement(59488..60909)
                     /locus_tag="IWI22_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9168001.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(60969..61961)
                     /locus_tag="IWI22_000584"
     CDS             complement(60969..61961)
                     /locus_tag="IWI22_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9168002.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
BASE COUNT        20005 a        11150 c        12600 g        18574 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggtttaac
    18901 tacaggagaa gatggaaaac tggcaattga cggtttagca ccaggagcat atcagctggt
    18961 agaaactcaa gcgccaatag ggtatgaatt agatgcgaca ccaatcgagt ttgagatcga
    19021 aagaagtcag acagcggtgg tagaattgac gaaagaaaac cgacttacac caggcggtgt
    19081 cgtgttgaca aagatcgatg accaaagcgg ggaaatcctg caaggagctg tttttgagtt
    19141 acagaaccga gaaggagaaa cgctacaaac cggtctaact acaggagaag atggaaaact
    19201 ggcaattgac ggtttagcac caggagcata tcagctggta gaaactcaag cgccaacggg
    19261 gtatgaatta gatgcgacac cgatcgagtt tgagatcgaa agaagtcaga cagcggtggt
    19321 agaattgacg aaagcaaacc gacttacacc aggcggtgtc gtgttgacaa agatcgatga
    19381 ccaaagcggg gaagtcctgc aaggagctgt ttttgagtta caagatgcca atggaaccgt
    19441 tctgcagtca ggtctaacca caggagcaga tggaaaactg gcgattgacg gcttagcacc
    19501 aggagtgtat cagctggtag aaacccaagc gccaacgggg tatgaattgg atgcgacacc
    19561 aatcgagttt gagatcgaaa gaagtcagac agcggtggta gaattgacga aagaaaatcg
    19621 acttacacca ggcggtgtcg tgttgacaaa gatcgatgac caaagcggag aagtcctgca
    19681 aggagctgtt tttgagttac aagatgccaa tggaaccgtt ctgcagtcag gtctaaccac
    19741 aggagcagat ggaaaactgg cgatagatgg cttagcacca ggagcatatc agctggtaga
    19801 aactcaagcg ccaacggggt atgaattgga tgcgacacca gtaactttca agatcgaaaa
    19861 agaacaagaa gtggcgatct ttttaacaaa agagaataga aaagttgctg atagctcaga
    19921 tataagagga aacaatccaa caagcagtgg aaataagcat cttccaaaaa ctggagaaac
    19981 catcattagc cagtttatat tgtcaatatt gggtgtgcta cttgttttca tcagtataaa
    20041 atttagaaag aaacgaaata tatgagctaa aataaaaaat tgaataagca tagtttttaa
    20101 aatcaactag tacccatcaa ttgaatggaa actaccgatt ttagaagggg tatacaaaca
    20161 aatattagta tcggagtaga gaaggttgaa cccaaccagt aaataagtaa agacaatttt
    20221 gtgctgcgtt gagaagtgaa aaataaacct ttgtagtaat actagttaat aaaaaatata
    20281 caaaaacact gcgaaaatca ttcttgagtt tcgcagtgtt ttttgcctaa tgactcaagt
    20341 ttattgggta atactttgat tttctttcta caaataatca gcgatcagtt gtaatttttt
    20401 ctctagtttt aatggctctt tgtcaataag gagaaaaaca agtcgggtct tttgtctcgg
    20461 aaagcttcaa ccaactcgtg aataaaggtt acgcctcctt gaaaggcgtg gaaaaaccaa
    20521 gtaatcgatc aatcaccaat ttcgcatttg gtagaacatg tttggtgagc tgaaagtagg
    20581 tggcgttcat gtctgtaact aagaattcta tttctttagg attagtgcag ctaaggaaat
    20641 agtttgttaa tcgaggaatt ttttgtgtag gtaaattttc tcgattaatt tttctgtctc
    20701 gccatccgcg gaaataaaac tcagtttatc ctcaacggaa acgtgagaac gaaattcaca
    20761 accctcaaca ctcaagacag aattcttttg gaagactttg gtaaatagct tctcaactct
    20821 ttcaatgtgc gaataacggt tgtcaaagat acctaacaac ttttcgaaat aaaaaataac
    20881 gacacttttt cagtcagtaa agaagtgatt ttatgtctga catgatttga aatcgagtgc
    20941 cttggacgga taaaataact ttgagcgttc caatggcttc gacagttttt acaggtatag
    21001 cgctgctttt ttaggcgtat aaccattggc atatgattat attattcaaa gtggacaatg
    21061 gtttccttct tcttgttttt caccaccatt aatttcctat ttccatctac cacagcagaa
    21121 ccacaatttc tacagacaca tggagcaggg aagagaactg cagcaacgat caatgttttt
    21181 ttcttctgta aggtttcgta agagacctct gtaatcatca aatttttgtc tattgatctc
    21241 agaatttttt tgatagaacc attcataaag catatcgttc tctcagttgt ttattttgtg
    21301 gtgatttaat catacaagaa aacgatatgt ttttttataa aaacatgaat aggaaaaata
    21361 gttgacaaga aaatgacaac gccatatgat gttccatagt atttacaacc tgtcatagat
    21421 gactgtaaaa aataaaagta aaaagaaagg acgacaacag gtggattttt atcagtctct
    21481 acaactagat ccgtttattt taaaacaaaa aatacgagaa gcagcttcta aaaaagaaaa
    21541 gtgcatgtat atttgttcat tgtttttacg atcattattc atcgtattgt ttgccatttg
    21601 ttttatcatt ataattacaa cattatttga atcgaagcat aaaccatacg ctgtggtttt
    21661 attctgtatg ttgatgagta ttcgttttgt tgactttggt tataagattt cccattcgat
    21721 cataggttta gcaatcgtca tgttctcact attagtagca ccgtatgtcc agctgatcaa
    21781 atggtcagtt atgggcatgt tgattcattt tattttacta agcagcatat taatggcaac
    21841 cgctagtgat cccaaaatgg ggaatgcttc tttgtacggc ttttcatatc tctttattgt
    21901 ttattcgtta cctaaagatt cgttgaacaa gaatttcttt acacaaacaa gtagcttatt
    21961 atttttgttt ttttgttggt tttcagttct cctttacaga aaacatcgag aaaagaacaa
    22021 agacaaaagc ttgtttaaag agattttttt gaaaggcatg tattcgcagg aaaaaatttg
    22081 gatgctcatc tatgcatttg gcattagtct tttgatcgtt gcaggagagt atgtcccttt
    22141 tcagcgattg atgtgggcag ggtttgcatt ttcttctatt gtatctagct atggattgat
    22201 gtcgattggt ttcaaagaac gagcagtaga ccgaatcatt ggctcattga ttggatgtgt
    22261 tttatttatt ggtatcagcc aatttattcc ttttaactgg gtcggtatat taggtggtct
    22321 agctttaggt gtttgttcta cctatagata taaaacagtt tttaattctt ttggtgcttt
    22381 ggcaatcact gccagtttat tcggggttcc aggagcagta acgatccgca tttttgagaa
    22441 tatccttggg gtatgtttag gaatcatgta tattggggtg actgaaatac ttatccgaaa
    22501 aataagagaa aaacacggtt tgaatcatta gatgagatcg aataaaaaat aaggaaaaag
    22561 tgaagataca catggatgat aaattgctat cattccatgt gtatcttttt acggtaaatc
    22621 agtgaaacgt tcgactttca tttttaattt atacttttta cggagttcgt ttatttgagc
    22681 catcatttcg gatttgtgat gcttttcgat tgcttcttca tctttccaac ggtcgatcag
    22741 taaaagagat tctggatctt ctagtggaaa gtaatagtca tatttctcgt ttccttcttc
    22801 tgcacgaatc gctttgacaa tgtttctttc gaccatttct tctgcaaatt ttctggcttg
    22861 tccatttgta cttgtataca aaatattcac tgtgatactc actaaatccc ctcctgaaaa
    22921 aattctatca ctagtatatc acggtgccag aaattattat tcaagatatg atacacattt
    22981 tttccgcaat gctacggaaa tatatatttt gaatagaaaa cgcttacaag atatgcttgg
    23041 tttataaaaa taaaaggagt gattgattta tgaaaaaagc attgattatt tgtgcggcag
    23101 gaatgtcatc ctcgatgatg gcctcaaaga caacggattt ctttaagcaa aaaagagaag
    23161 aggtactcgt agatgcagtt tcagctacag aaggcgataa tatgattaag aatagtgatt
    23221 ttgatttatt tctgatcagc ccgcaaacga cgatgttttt ggataaattc aaaaaattag
    23281 gagaatctgt tggaaagcct gttgttagta tccctttcca agcgtatgtc ccaatcccta
    23341 caggcatcca gaagttagca gaggtgatcg aagagaacat atgatggaat aggagtggat
    23401 gataatgaaa cagaaggaaa aattgctgtt gagatatttg attgatcaca agaaggagta
    23461 tgtcaccagt caacgtttag cttctgaatt gttattatct gatcggacaa tccgtaatta
    23521 cttgcagcgg atcaaagagt tagtagaaaa aaacggaggt aagatcattg ctaaaccagg
    23581 ttatggctac caactgcata ttttacaaag actgacattt gacttgtttt tgagtcggca
    23641 agaaatagta cctggatatg ctagagaacc gcaaggattt tatgagtccg aagatcgaca
    23701 aaagtatata ttgaaccaat tgcttttgga agatgccatt ctttttatgg atgatttggc
    23761 agaagagctt tatatcagtc gttccagttt gacaaaggac atgcaagaaa tcaaggagcg
    23821 attgattcca tattcactaa aaatcgtttc taagcatggt caagggacat ggatcgaagg
    23881 ggaagaaaga aatcgacggc attttatcat ggatactttt tttggaaacc attatggaaa
    23941 ttcattgaaa gaatatttag ggcaaagccg ttttttccca gacatcagtt ttgaagagct
    24001 agtcattatc attctcgatg aaacaagaga agctaagtta aaagtttcgg attttatcat
    24061 acagaatctg actttacact tggctttggg gatcaaacgg ctccgagaag ggtttgaaat
    24121 caaggaatta ggcatcgaac aagaagtttc taagcgagta gagtatcaag tggcgcagcg
    24181 gatcgttcaa cgtattgagg taatcgcagg tgtatcgttt cccgaagaag aaattgctta
    24241 tttgacatta catctcatgg ctaagtcgaa tcacaaagaa aatcaaaatg atcaggaact
    24301 atcaaaggaa ttagcgaaag ttctgaaaag attgtctcag gaatttcatt tttcattagt
    24361 agaggatctc cagcttttca atggactact cgatcatttg tcaccgatga tgattcgatt
    24421 gagccgaggt attcgcattg agaacccatt gactgaggag ataaagaaag aaaatcccaa
    24481 agtctttgac gcagtaaaaa ggcatttcag caacatgccg gctttgaaaa actataccat
    24541 caatgaagat gaatgggcct atttggcttt gcatctcatg gcggcattgg aaaaagaaag
    24601 agctgcgcat aaactacatg cattgattat ctgtgcaacg ggttacggca gtgcccaact
    24661 attaaagaat cgcgtagtca gtgaattcgg taaaaatatt acagtggtaa gtgtaaaagg
    24721 ctattatgag atcaatgaaa caactttaaa aggaatcgac ctgattattt cttctattga
    24781 tctttcgaca atgtttttca aaattccagt tttacatgtc agtatttttt tgaatgaaga
    24841 tgatgttcgt aaaatacgaa aggtagttgg agaatctata ccctatcatt cctcagatag
    24901 accgatacca gcattttctc tacagcaaaa aaagcagatt tacaccgaac agttgtctga
    24961 aaaatttttc aagacttata cgtacgaacc gaaaaaggaa gaaattcttc atgatttgct
    25021 gcaaaagctg agcataaatg aagaagaagg atatataaga gaaatgcgaa agcagatcca
    25081 gcaacgtgaa aatatgggac aaatcatatt cagtgataca gtggcagtcc cacatcccgc
    25141 tgttccagtt ggaatcgaaa caaaggtagc cgttgcattg attccttctg ggatgcagtg
    25201 ggatcaatat caagaaattc attttgtttt tttagtttcg ccttcttatg tcgaaaatga
    25261 aggaattacg gtcgtgacta aagcaatcgt taggttagtt gaccagctaa aagtacagca
    25321 ggaaatctta gctgaaccta tttttgagaa tttcagcagt aaatttatca aactgatcga
    25381 ataaggagga aaacagatgg ctgaaaaaat aaacagtgaa acactacaag tgacttcttt
    25441 tgatatcatt ttccatagcg gaaatgcacg gacactgata catgaagcat ttcgctcgat
    25501 gcgtaaagag atgtttgaag aagtagaaga gaagttgcga caagcaaatg aagagattct
    25561 tcatgcgcat cagtcacaaa ctcaacttct gaaagaatat gccagcggga caaaaataga
    25621 aatggaaatt atcatggtcc atgcacaaga tcatctgatg acgacgatga cattattgga
    25681 agttgcacag gaaatgaatt atttgtatca aaaaaataaa tagaaaggaa ggatcattgt
    25741 gcaggataca gtaaaaagga aaatcgaatt agaaaaaaag caacttagtt tgaaaaacat
    25801 gtatttcaat cgttatctaa tcgtacgtta cctgactgcc tttttctttt tcatgaacat
    25861 gcaatggatg attctgcttc tatcagctaa aagtttagga agtagtttac cgatgattct
    25921 gattttagct attttgcctg cagtaggaga acaagtaaag ctttatcgaa aacaccaaac
    25981 gaacgttcca tggacaaagt ggtatttttt gttccagagt gtctttaatt tattagtgat
    26041 tccagtgttg tttacctcag gatttacatt cttgtatcct tttatggcaa ataacaatgg
    26101 aggacagtta tttgttttca ttcttgttgt aagcggtatt tttgccagtg catttataca
    26161 gtatcgttta aagaaaattt cactcaacca agaccaacag tatatccgta ttaaacaata
    26221 tgaaaaagcg ttatatttag gaaaggagaa taactaatgt ttgatttttt gcagaaatac
    26281 ctgatgggac ctatggggaa aattgcgcaa cttaaaatcg ttcgggctgt catggcagcg
    26341 gggatggctt ctattccttt cacgattgta ggctcgatgt ttttggtttt aaatattttg
    26401 ccattaccgt tcccattttt agaaggattt tttaatgcaa cttttttcaa agttagtgac
    26461 ttgtacatga tcgtcaatac aatgacgatg gggattttat ctatttactt tgccattgtg
    26521 tttgcttatg aattaacgtc aattgaacga gatgaacaaa aattaaatgt taatccgctc
    26581 acgggggcat tactatctgt ttttgcattc tttatgtgta tccctgaatt gatcatgact
    26641 gatggtaaaa tcagcttgat tgcttctatc aatgagacag aaactgtagt aaatggtatt
    26701 aggatgggct cttttgtcga acgtttagga acttcaggaa tctttacagc tattattatg
    26761 tcgattattg cggtcgaatt gtattgtatg tgtgtcaaga gaaattgggt cataaaaatg
    26821 ccagatgttg ttccgccagg agtttctcgt tctttcactg ctctgatccc aacatttgtc
    26881 attgcgtttg tggtgatgat catcaatgga ttattagttg cactaggaac ggatatcttc
    26941 aaaatgattg ctatcccatt tgggtttgtt acgcagttga cgaatacatg gcttggtgtc
    27001 atggtgattt atttcttgat ccatgcattg tggatcgtag ggatccatgg agcgaatatc
    27061 atcacttctt tcttgacccc gatcgtatta gctaatatgg cagctaacgc acaaggcgca
    27121 aattatccgt tggcaggaga attcaacaat tcttatgtaa ccgttggtgg atcaggtgcg
    27181 actttaggat tgatcatctt tattgcattc ctcgcgaaat ctgatcagct gaaagtgtta
    27241 ggaaaagcct cacttgtacc aggattattc aatatcaatg aaccaatcat tttcgggatg
    27301 ccaatcgtct acaatcccta tttagcggtt ccgtttttct tagctccgat ggcttctgct
    27361 acactcgctt attttgccat tcggctggaa atcgtcaaac cgatgcttgc acagatgccg
    27421 tggccttcac cagtcgggat cggagcattt gttggtagtg gtggtgattg gaaagctgct
    27481 gtgttagcag tcatttgtgc aatcatcgca ttcattatct ggttcccatt tatcaaattc
    27541 tatgataata aattattaaa tgaagaaaga gaaaaggcag cagaattagc tagtgaaaag
    27601 ttaaaatgat ttctaagaaa aaaggaactg tgacaaaaaa tcacagtccc tttttttctt
    27661 agaaatggga aaccactagt atataaatac cattattctt caatcaaccg cttcacgatt
    27721 gcataatccc cttcataagg ttcatctaca ccattgattt tttgataggg atccacgcct
    27781 tttcccgcaa taacgactga atcttgcgga gcgctttctg ctaaagcgcg tcgaatcgct
    27841 tcaggacgat ccatttcata atgaacgatc aaatctggat tagtaattgc ttcattgatt
    27901 tcttccgcga tttttttcgg atcttcaaat gctgggtcat ctgccgtcaa aaaggcgatg
    27961 tcagctgttt ctgatagcac ttttccaaaa tcatgtcgac gggagattgc tttgtttcct
    28021 gggctgccta atacaacaat tacacggcca tctggatgtt catttttggc aaatttcagc
    28081 aatgttttca agcttagata attatgtgca tagtccacat agacatgcgc tccattggat
    28141 tggatcaatt gatccattcg gcctggtaca cgagcttctt ttaatccgtc ttgtgcatct
    28201 tgactggagg cacctgttag tgctgcggca atgatcgcac tggctgcatt ttcttggttg
    28261 aatcctccag ccagcaaaat atcataagca gtatcgcaga tagctaagcg atcttcatga
    28321 gaagataaag taaaaccgtg agtaccttta tctgaacgga tcacttgata attgctttct
    28381 tccgaacgac cataagtgat cgttggaatc tggaatagat cggctgtttc cctaagtaaa
    28441 tcaaaatagt cactttcatg atttaaaaca actgtttttg aattcaagat caattgtcgc
    28501 ttgcaataga aatagtcatc aaatgtcgga tgttcgatcg gactgatatg gtcaggcgag
    28561 atattcaaga agattccgac atctaaagtc aagccgtata cgcgttgtgt tttgtaagct
    28621 tgtgaagaaa cttccatcac tagatgagtc ataccatttt caactgcttc tgacatcata
    28681 cgatataaat ctaatgactc aggcgttgtc aaatgggatt tgaaatatgt tttgccatct
    28741 aacgttgtat tcatagtaga aagcaatgct gtttttttat ttgttgtgtg atccaagatt
    28801 gcttttgtaa agtacgcagc agttgtttta ccttttgttc cagtgaatcc gatcactttc
    28861 aatttatttt gcggatagtc gtaaaaagcc atagcaagaa gcgccattgc tttacggata
    28921 tccgttacga tgattcctac tgctgaattt tcgtatggtt gttctgatac ataatattga
    28981 ataccctgtt cgattgctga atccaaatat tcttctttga aattcattcc tttacagaaa
    29041 aacaaagtag aagcatcagc ttttcttgaa tcataactta actgaagaaa ttcagctttt
    29101 tctggtacat caagatgcca gcctcttgtt gaaacaaatt cttttaatag attttctttt
    29161 aatagtaaat gacggatgtc ttctaaagag aagctcattg attgatccct cgctttattc
    29221 aatttcttgg tttattatag cacactcttt ttaatatatc gtttgtttcc gctatgacaa
    29281 aatccagctt tattcaaatt ttcttgacac aggggacaag cggatgagta ttttcataaa
    29341 caatgaactg actgtaatag ataggagaac gaatagtgtc tccaacaatc caaaaacata
    29401 ataattcaaa aagacgataa gactattggt aagtaaaagt ccttgcggca ttggaagccg
    29461 aaaaaatttg tggaaaataa tcggcggtat cgttataccg ccatttgagg ccccgactgt
    29521 gtacaatgtg taaaccccaa gactgaacaa aagacttcct gaaatcagtg aaaagaaatg
    29581 tgaggagatc atttccactt ttggaacaat ctctaaaaca acaggaaaaa gcagacttcc
    29641 cgctacagtc ttaaaaaata ctgattttcc taataggaca gcggctagaa gcagcatcat
    29701 aagattgatg ctccaaacag taactgagcg ttggatagaa aaggccgttt caactaaatg
    29761 tccgatggcg cccactccgg cggaagctat atcgtgaggg agcaagaacc aattcaatcc
    29821 aactgtgata aagaaaagac ctaaaaatat aacgggtagt ttttttattt cttgtgtcat
    29881 tgatatctcc tataaatgtt agccataaaa ggctgtatat ttctttactt taccaaaaaa
    29941 acgaaaccga attcaaaaaa acaattgttc ttataaaagt tatataaaat cgattgccag
    30001 tttctgtttt accaattaaa atgatagtaa tgttttgtag aaagttggaa tctgaccgat
    30061 ggataatcaa agaaaaccag cattagatca attaacaccg caagaaaaaa tggcacgagg
    30121 ctctgcttgg atgaccgcca gtaatatcat ttcaagattg ctaggcgctg tctatatcat
    30181 tccctggtat gcttggatgg gcgaaaatgc taaggctgcc aatggattat tcaatatggg
    30241 atacaatata tatgcattgt ttctactgat ctctacagca ggaatacctg ctgcgattgc
    30301 aaaacaaact gcacgctata attcattgaa tgaatatggc acttcacgac gattatttat
    30361 ccgtgcatta cagatgatgg gcgggttagg cctgttgttt gctttgttca tgtacatcgc
    30421 ctctccttgg ctagctcatg cttctggtgg cggagaagaa ctgattccaa cgatgcgttc
    30481 tttgagtatc gctgtattgg tttttccttg tatgagtgtg atacgagggt atttgcaagg
    30541 caatcaagaa atgatgcctt atgcgctgtc gcagattgta gaacaggtgg cacgtatttt
    30601 ttatatgcta ttggctacat tcatcatcat gaaagtcatg gatggtgact atgtgacggc
    30661 ggtcacgcaa tcaactttcg cggcatttat cggtatgcta gcaagtattc ttgtcctttt
    30721 gtattttttg aagaaacatc aagcgtatac atcggcgttt attcactaca gcgaaaacaa
    30781 aataaatatt gcaacgaagg aattgttgtt agataccgtt aaagaagcga ttccgtttat
    30841 tattgtgggt tcaggggtaa cgatcttcaa attagtcgat cagttcactt ttatgaagat
    30901 catgtctgca tccaccgagt attccaatgc acagttactg gatctttttt cgatttttag
    30961 tgccaatcca gataagttga cgatggtagt tattgcattg gcaacatcga tcgcgtctac
    31021 tgggttaccg ttgattacag aagctgttac gttgaaagat cgagtaggtc tggcaaaatt
    31081 gacaagcgga aatttacagt tgttttcatt ttttatgttt ccggcgacat ttggtgttat
    31141 gttacttgcc tatccgctaa atacattatt ttatacaccg gattctctag gaagtaatgt
    31201 actgatccaa gcaagttttg taggactctt cctaggacta tatatgctag tttccaacat
    31261 gctgcaagga atgtttgaaa acaaggcggc aattaattat ttggttattg ggtttctagt
    31321 gaaactcctt cttcaatacc cagccattcg tttatttgaa gtatacggcc ctttattggc
    31381 gacaatgatc ggatttgctg tgtcttgtac cttgatctta aagagaatcc atgaagtagc
    31441 ccgctttaaa ccaagttttg tctggcgcag gacattgctg atctttattc ttaccttgat
    31501 tatgcttttg gcttcgtgga tcacaaagat gatttttggt gcttttttaa acgaagatag
    31561 caaattccag tctctattgt tgattcttgt agtagcaggt ataggggctt tagtgtatgg
    31621 ttatttagct ttgaaaatcc gcttggcaga aagactacta ggaccaggaa tagcgcgttt
    31681 acgacataaa ttaaaaataa aataagtgaa tcagaagagc tagattgatg ctcttttgaa
    31741 atgggggaaa cgcatgcgtt tagataagtt tttagcagat atgggacttg gcagccgcaa
    31801 agaagtcaaa ggattaatca aaaaaggaac gatcgaagtc aatggacaag tggtgaaaag
    31861 cgataaagcg caagtaaatg aattcgaaga tgcggttact tatttaggag aaatcattac
    31921 gtatcaaaaa gatttttact acattttgca taaacctgca ggagtcattt ctgctactca
    31981 ggataatcat gatcaaaccg taatcgattt attggaagac caggattatc gggaagatct
    32041 ttttccagtc ggccgattag ataaagacac agagggcctc ttgattttaa cgaatgacgg
    32101 ggtgtttgcc catcagttac tatcacctaa aaaacatgtg gagaaagaat atctagctga
    32161 ggttagcggt ataatgacag aagaagatat ccgtttattt gcagaaggac tgattattga
    32221 tggcgatgag cagacattgc cggcaaaact ccaaattgat caagtagatc aagagacaga
    32281 aacttcaatt atccgtctga ttttgcatga aggaaagttt caccaagtta agcgaatggt
    32341 caaagcagta ggaaaagaag tcttatattt aaaaagaatc cgtatgggaa aattttggct
    32401 gcctaaagat ttgaataaag ggcaatatcg acccatgacc aaagaagagc tagaacaagt
    32461 gagagaaaaa taattgaatg gatagctaaa agaataagca gagtagttgt agcgccatct
    32521 gaaaagcaga cttgtccgtc tgactttcgg atggcttttt tgtgaaattt cggcttattt
    32581 ccgtagaagc tacttctgga acaccgtttg ttcggaataa gggactgtga caagactttt
    32641 gttacagtcc ccattgttcc ttgtgactca gagttagata gtaaggacac gatctcccta
    32701 atactcaggc caaaacgctg aattcacaac ctctgataaa cggtgttcaa tcagctgacc
    32761 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttcccgtt tttttctcta
    32821 atactcaggc caaaacgctg atttcacaac ctctcctata ctttgtttca ttttccctat
    32881 actttgttta tttattctca ttattgaaag cgctataatt acgttgcgaa tgaatcgaaa
    32941 ggggttttta gatgaaaaaa cgaaaggggt tcaatcattt ttttatgaat ctatggcgtt
    33001 ataaagcact tgtcctgatg gctgtaccgg gtatgctttg gatgattttt ttcttttaca
    33061 tacctgtaat ggcgaatgtc gtagcattca aaaattttca tatttcagca gatgggttct
    33121 tggctagctt gagggaaagt ccttgggtgg gttgggaaaa tttcaaattt ttattttctt
    33181 cggatcaagc atttctcatc actaaaaata caattttata taatgtagca tttatccttt
    33241 tgaatctgct gatctcagtc gtatttgcaa tcattatgag cgagttgaga aataaacgac
    33301 tcgttaaagt ttatcaaacg atgtccttac taccttattt tctatcttgg gtcattatca
    33361 gttactttgt ttacgctttt ttaagtcctg acaaaggaat attcaatcag tggatcactg
    33421 gacatggagg agaagctgtc aattggtata atgaaccgaa atattggccg tttatcttgg
    33481 tgttcattgg tacatggaaa gggattggtt ataacagcat tatttacttt gcatctgtta
    33541 tgggaatcga tccgacttat tatgaagctg ctatggttga tggagctagc aaatggcagc
    33601 agatcaaaca tgtgacgata ccacaactta ttccactaat gacgatttta acgattttag
    33661 ccgtaggaaa cattttccga gctgattttg gcctgtttta taacattcct aggaattccg
    33721 gtgcattata tgatgtgact caagttttgg atacgtatat ctacaatggc ctgacatcca
    33781 caggagattt tggtatgacg gctgctgcag gactttacca atcagttgtc ggctttgtct
    33841 tgttgatgat cacaaatacg atcgcccgac gttttgatag cgattctgca ttattctaag
    33901 gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa gtccgctctt tcaataagac
    33961 ggctaatttt ttctctaata taatgattgc actctttgct atttcctgta ttttgccatt
    34021 tatatttgtt atcgccatct cttttaccga tgagtccata ttgtcgactc acgggtacag
    34081 tttttggcca gaaaagttca gtatatttgg ttatacgtac ttgttcgacc agatgcagga
    34141 taaaattttc caagcgcttt tcgtcactgt acttgttact gttttaggta cactgatcaa
    34201 tagcacggct acttctttat atgcttatgc gatttcacgg tcaaactttc cattccgtcg
    34261 tttttttacc gttttttgtt tgattacgat gttgttctca ccagggatgg tggccaatta
    34321 tcttgttatg acaaatcttc ttcagttaaa agatacgatt tgggcattga tcttgccaat
    34381 ggctgtcagt cccttcaata ttattgtgat gcgcacattc tttaaacgtt cggtctctga
    34441 ttcgattatt gaatcggcac ggatcgatgg agctagcgaa ctgcgaatat ttgttcagat
    34501 cgttttaccg ttagctgttc caggtattgc gacaatcagt ttatttgccg cattgggtta
    34561 ctggaatgac tggttcaatg ctttactata catccaagat gataagctag ttccactaca
    34621 gtatcttttg atgaaaatcc aaagtaatat tcaatatctg acacaaaatt caggagcagg
    34681 aagccaattg acaggcggat tagcctcagt tccaggagaa tctgccagaa tggcgatcgt
    34741 agtcatctcg acgttgccta ttgcgatcag ctatccattt ttccagaagc attttgtcaa
    34801 aggtctaaca atcggcggag taaaagaata aagaaacaaa aatataaatc ataaaattag
    34861 gaggaagaaa aatgaagtta tggaagaaac tagcctttac aggtgtttca gcgttgttgt
    34921 tgggaacatt agcagcatgc ggaggttctg gttctaaaga ccaggcagaa gctagcaatt
    34981 cggacacttt gcagatgtat caaatcggag ataaaccaga taatttcgat caattgatgg
    35041 aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa agtcaatatc aattacattg
    35101 gctggggcga ttacgaaaag aaaatgaacg tcatcatttc ttccggggaa aattatgata
    35161 tcgcctttgc gaacaactat gtttccaatg cccaaaaagg agcctttgct gatttgactg
    35221 aattagcgcc taagtacgct gaaaaagctt ataatgattt agatgaagca tatattaaag
    35281 gaaatctggt caatggaaaa ttatatgctt tcccagtcaa tggaaatgtc tttgctcaac
    35341 aagtacttac ttttaacaaa gctttactag ataaatacga tctttcaatt gatggaatcc
    35401 aatcttacgc tgatgcagaa aaagtcttga aggaattcca tgaaaaagaa ccaaacacgg
    35461 cagcttttgc cattggacaa ggcttcaaag tccaagggaa ctttgattat cctttaggga
    35521 atacgttacc atttgctatt gatctggatg gagatgattc gaaaatcatc aaccaatacg
    35581 ataatgaaac gtatatcgga ctgttacgta caatgcatag ctggtacaaa caaggactca
    35641 ttccaagtga cgctgcaacg agcaataagg attatccact agaaggaaat acttggctaa
    35701 tgcgtggaga aacacaagga ccgtatgatt atggcgatac aattttgaca aatgcagcaa
    35761 aacaagaact tgtctcaaaa gcaattactg ttccactaaa atctacagct caagcacaaa
    35821 tggcaaattt tgtcgtatca aatacttcaa aaaataaaga aaaatcagta gaacttctag
    35881 ggctgcttaa cagtgatcca gaattattga atgggcttgt ttgggggatc gagggagaag
    35941 catgggaaaa agtcgatggt tcagatgata aaatcaaatt gctagatggt tatcagccaa
    36001 atacacatat gtctgcttgg aatacaggaa acaacaagat tctatataca caagagtcta
    36061 ttacggatga catgattgcc aaacgtgatc aatcgattgc tgatgcagaa acttcaccga
    36121 ttttaggatt cagcttcaac actgacagtg tcaaaacaga attaagtaat atttctaatg
    36181 tcatgaatca atatttagat ggattgaata cagggactgt tgatccagat gaaacattac
    36241 cgaaattgaa agacgctttg gacaaggctg gctatgataa ggtattgaaa gaaatgcaaa
    36301 aacaatatga tgagttccga caagaataat aatgaataaa gacaaagggg ttgtgacaaa
    36361 agacgtgtca caacctcttg ttccgaataa acggtgttca aaagccagtt tctcggtatt
    36421 aagctaaata tatgtaaaaa tgtggaaagc catattcaca aaatttttct tactactcga
    36481 aactaagcgg ctatttcaca aactcttggt aaacggtgtt caatcagcta accctcggta
    36541 ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc gtttgtttct ctaataatca
    36601 ggtcaaggtg ctgattgccc aacctttttt tgtataatga gaaagtataa acttaatgtt
    36661 tggaggagaa gatatgttag ggaaggtttt ggaagagtta tttatccgga tctgggtcat
    36721 tataaaacta accttgtatt tctggatata tacatttgcc ggcgggatca tctttggatt
    36781 aggtgcggca tggaaaacag tcaatgaact attttatttg tacggattcg agtataaaga
    36841 aatcacaata aaaagaggct ggaatatata taaaagaaat tttttaagag gcaatctgct
    36901 atttagttta tttttatctg gtactgcttt attgagttac aatttatatt tatctgttca
    36961 aatcaaaggg ctcatttttt tagctattga ttttatttta ctttttgcct tgctctgcct
    37021 atttgctact tatcaataca gtatgatttt agatagtgaa tatacgatct ctgttcctaa
    37081 tctgctaaag ctaagtttta tttcggtttt ttccagcttt tcgtcttttc taaaaataat
    37141 tattggtagc ggtataattt taggcgtgac ttggcaattc aaaggattga tcctgtttgg
    37201 tgtcattggt cttttgacgg tttggaatgg tacgatgaca acacattggc gtgaagaatt
    37261 agataaacag ttggaaagtt atgaataagt ttcggattct attttcaaaa ggaaccttac
    37321 taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg tgtaacgatg actgctaccg
    37381 ttatttttag tatccatact tgggaacaaa atcaaaaaca ggcagaaaac atgacaagtg
    37441 atgccgttca aagcacaagc agaatgctga atgataaaac aaccttgagt aggatcatca
    37501 aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt gacgacctac ttaacaaaac
    37561 ccatagatca gtatctgatg tatgtatacg aacagcaaaa cagtaccgat gaattagttt
    37621 ctttcccaaa tcaaatcaaa gatctctacg caaattatga agaactaagt gcgatatata
    37681 tcgtgttgaa tcaactaccg gagtattatg aatcttctag agaaaacaaa ggtggggaaa
    37741 taaaaaaggg gacaccaaaa ttactagacg ccttttatat caagttgcct atcacacaag
    37801 gaggagctga gtcaggaagt atttttattg gttttcaaaa gaaagaacta gatatgattt
    37861 tagaaaatct aacgtcattc aatggactct ctttatacat gatttctgga acgacaaatc
    37921 gactatacac attccatgat aataaaatag ctaaagaaac gtttgaaaga caagaaatga
    37981 tcatcacaga gagtctgaag aaaaattcac aattgccgat tgacgatctg gaaaaaaaca
    38041 attttgttca acaccaagaa ctgtctgatg attatcgtat attagctgta ctagataaaa
    38101 aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct gatcattggt atcattattt
    38161 tagacaatat actgcttatc tttctttata aaacattcaa acgatattca cagcaagtat
    38221 ctatcgtcat gcaggccatg gatcaaacag cagcagggaa tttagagacg cggatcgaca
    38281 taacaaacac ggaatatgaa ctgaaggaac tttcaatcgg tatcaacgaa atgttggata
    38341 gcatcaatca gtttgtagaa gacatctaca aattagaaat caaacagcaa gacgcacata
    38401 tgcgggcgct tcaagcacag atcaatccac actttttata taatacatta gaatatatcc
    38461 gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga tgttgtgtat gcgttttccg
    38521 ctttgcttcg aaacaatacg aatcaagaga aaacgatcac tttaaaagaa gaactggact
    38581 tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc taatcgagta gcctatcatt
    38641 ttatgatcga tcctgatttg gaaaagatcg aagtacctaa atttgtgatc cagccattag
    38701 ttgaaaatta ttttaaacac ggtattgatt ttactcgatt cgataatgcg ctaagtgtca
    38761 aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa agataatgga aaagggatga
    38821 ctgaaaaacg gctaaaacag attgaagaaa aactgtctca tcctaaagtg gaacttcacg
    38881 gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc cagcttcggc agcagttatt
    38941 atatgtcgct tgagaataat gagacaggtg gactgaccgt ttctattaca ttcaaggagg
    39001 gatgagcgtg tatcaagtgt tattggtaga tgatgaatac atgattttga atgggttaaa
    39061 aaagataatt gattggcaaa gtttaggctt tcaaatagta gcaacagcag agaatgccaa
    39121 agaaggacta gctgtacttg aacagcgaca gattgattta gtagtaacgg atgtgacaat
    39181 gccagaaata aatggactgg aatttattga agcggctcaa aaagaacgtc ataattttga
    39241 atttatgatt ttatcaggat accaagaatt cgattatttg aaaggaggta tgcaattagg
    39301 cgccgtgaat tatttgatga aacccgtgaa taagttcgaa ttggttgaaa gcctcaagaa
    39361 aatcaaaaca cggttagatc agcaaaatga acaaaaaaat caacaagaaa tctatcaaga
    39421 actgttattc agtcaatggt tgaatgaaga attggatgaa ataagtgaag aagaaataat
    39481 aggacaaata agtgagaagc agcataggat agtattgatt caactggcaa gaactcatga
    39541 tactcttatc aatagatggt tgcaagaacg gcaacaaatc ttttactatc aacgaaatta
    39601 cggagaccgt gtattgatca cgttgttgtt ggaggcagat gaagttgatg gcttctgttg
    39661 ttttgttcaa gaaaactttc ctgatcagga atggctgatc agtatcgggg aagagacaga
    39721 ttctttggac aaaatccctg aaacattcca acaagcgaaa gacaatctac agttgcatca
    39781 attttatgga gataaagacg agcatgtttt ttacgcagta caagcagaaa caaaagatca
    39841 aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat aaacaattgg aagtcgctca
    39901 aaaaatgatc actgattttt ttgaacagtt tcagttagca gccatgatgc cggaagatat
    39961 acgatattcc agttttttac tgtttatgga gattcaacgg gaattgattg atctggaaga
    40021 tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca aaaacggtgc aagaacttca
    40081 gcagctactt ctgtcattca tcaaaaagca tcagagacag aaaaagtaca gtgacaatgt
    40141 ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa ccacttacac tcaaagaagt
    40201 gtcagaaaac ctgcatttaa atgtcatgta tctaggacag ctgttcaaaa aagaaacgaa
    40261 aaaaagtttt tcagcttatt tgaaccattt acgtatggaa aaagcgaaac aactgctttt
    40321 gcacagtaat caaaacatca atgagatagc aagtgagatt ggctacaaca acaccacgta
    40381 tttctctaaa ttattcaaga aaatcgtcgg cagatcccct aaagagtacc gagaaaacag
    40441 tggagaatca taaaagaaaa agccaatgta atgaaataac ttataaaaaa ttgtctagaa
    40501 atctaatttt gggctgttct tattacttga ggcaaggcag ctttttcatg acctctcgat
    40561 aaacggagtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtggaaa
    40621 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca cgacctctcc
    40681 actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaatggc aaaatatgag
    40741 gaactatttt gccatttgtc tctctaatac tcaggtcaga acgctgattt cacaacctct
    40801 caataaacgg tgttcaaaag cttgcatctg cggtaataag accaaccaaa cgaaaaatat
    40861 gaggatctat ttttgcttgg ctgttcttat tacttgatgc aggacagctt tttcacaacc
    40921 tcttgttaat tatagtttga taaggaaaaa ggcaaatctt ttgcttgata tttcttaggg
    40981 tgagggatga tccctaattg gtaagagatc gtgccgcctt tcagcaaatc ttgatgggta
    41041 aaatataatt tcgtatattc tttgtccatc cgtttgattt tatggataaa ttgttgctgc
    41101 ggccgattag gcgttgcttc aatcgtcaat tcctttccat ttgacaaaag caaaacagcc
    41161 ttgtcaatta atggcatacc gataacatat tcaccggttc caggcgtgac aggataaaat
    41221 cccagactat tgaagatata ccaagcggcc atacttccat tgtcctcatc tccagggtaa
    41281 cccattggag aatcgtcgaa ggtctgagtc atcaactgtt taagcagtgg ctgtgccatt
    41341 tcaggttgtc ctatataact gaacaagtaa ggatagtgaa aacttggctg gtttgaaata
    41401 gctaattgtc caaattctat cgcggccatc tcactcattt cgtggatctc aaaaccatat
    41461 cctcctgggt caaacgttgg gggctgattg cacagctcaa tgagtttttc tttatatctc
    41521 tcactgccac catattttcc gatcagtcct gaaaaatcgt gataaacagc aaagctgctt
    41581 tgccaagcac ttccttctgc ataatcttgt ccccaacgat tagctgaaaa tggttctctg
    41641 aacttgccat ctttatcttt tgaacgcata aatcctgact catagtcaaa gatattttga
    41701 tagttcaatg attgctgctg atatttcttc gtcatttcct cttcttctag taccttggca
    41761 acttggctaa tacaaaaatc gctataagca tagtctaatg tgtgattgac tgattcgtgg
    41821 tagtgattag gaacatagcc atatgtcaag taatcttctg ttccttggcg tccataattt
    41881 ggatgtttgc tttgaaccgt agcacctttt tccattgccg ttagaaattc aggcatcaag
    41941 tctcgtcgga tatcttttac agcagcatct gcaatgaccg catcgatcaa tgtcccaggc
    42001 ataagtccac gttcatcagg agacagccat tttggcaaat agccactttc tctatagctg
    42061 ttcaaaaaac cttccagcat ttcttcgtat ttttctacag caatcaaaga ataaagagga
    42121 taaaccgttt tatacgtatc ccaaaaccca ttatttgtat agagaacacc aggttttata
    42181 gatcgactag tcgtatcata gtggattttt tgcccactag gatcaatttc ataaaatgtt
    42241 tgtggaaata aaaacacccg atataaatta tgataaaacg tccgcaattg ttgttgatca
    42301 tggtgtgtga gctgtatacg atcaaaatag gactgccatt gttcttgact gtgagcaagg
    42361 taagtctctt tgctttggtt ttgttctagt tccaggttat aagctgcttg ttccgagctg
    42421 ataaaagatg tcccaaatcg gatagtttgt ttttttgtct gttcaaaata caacgggatc
    42481 gagtcgctat ttttgttgga aacagctaac tcatcaattg ttaaaggata ttcaaatctc
    42541 aatataaaat aaaaagaaaa atcaggatct tctgaaccag caaaatgtgt gatttttcct
    42601 tccactgtgt aaggatctat aacaaagagc tgatggcgtc ccggaaagga taaaagtaaa
    42661 ccgctgtcat ttttgctgta atcaatagat aagattccgc catacatgct aggtatcaat
    42721 gtagaacaga tgccataacg taattgacag atcgacagat gagtcgggca aaatatactt
    42781 tcttctgggc ggtaagaact ttgagcatgg aaaagggtgt tctcctgtag ttttccattg
    42841 atcggcgtaa acagcatatg actgaaatca cccatccaag gacttggttg atgggtaaga
    42901 cggtagcctt gaaaaacgcg gtcatcagga tgaaaccacc agctgccgtt ttgatccgta
    42961 gtttgcggtg caaaataatt catcccaaaa ggaacgccag tataaggctg gcagttccca
    43021 ttagagaaat ttggctgatt cgctgttgca tgtctagtat ctatttgacc gatattcatt
    43081 ttatttctcc tttgttgatt gtcttttgat gaatgaaact ggaaatttac tatcggatgg
    43141 tcgatctttt gtctctaacc aagtgaccaa agcatctccc gcaagcttac ctaactgttc
    43201 aaaatcttgt gcgatggttg tcagtggcgg gtcaacaaga gctgctgctt ggatatcatc
    43261 aaatccgata attgaaaaat cttcagggat atgataacca tgctgtctca agagattcat
    43321 ggtctggata gcgacaacat cgttttcaca aacaaaagca gttacttcat acttagtgat
    43381 ataagaaagc aattcatcta atgtcgcgct tttgtcatcc agtgaagtgt ggaaagtcaa
    43441 accagctttg tccaatgctt caaggtaacc aaggtaacgc tgtcgaacgg attgtggatg
    43501 agtctgcgta ccaaacaaat aagcgatttt tgaatgtccc tgttcgatca aataagaggc
    43561 agcaagcgca ccgccttgga aattatcgga aagaacggta ggaaaaggta actcatagat
    43621 ttttttatct aatatgacga cagggaattc tttcaatgaa agctcaaaaa gtaagtcaag
    43681 atgttgttct gtatcaagag cataataaat caatccgtca aattcattga ttagctcttt
    43741 tgctgtttta ttttgtagaa agtcaagcgt cgtcatcatg acatcaatct gtttatcttg
    43801 catcacaggg ttcaatccct tgctaaaatc accaacagac aaatcagcga catatggaag
    43861 aagaaacaga atccgtcgtg tttttgtagg agaagcaaga atctgttccg gagaagcgga
    43921 tttaacgaaa ctgcctttcc cgcgaacgcg ataaataagt cctaattgct ctaattcagt
    43981 caatgcacgt ttggaagtga ttctgcttac tttataggta cgtgaaagtt ctttttcagt
    44041 tggtagttgg ctgtctacag gtaattcacc agttaaaata gccgtttgca aatcattaaa
    44101 tattgtctgg tacaatggtt gattcatata aacacctctt aatgatatat catattcaaa
    44161 taataccata atggatgaaa aagtaaataa aaagatattt tttgaaagcg tagacataat
    44221 tggctgtatg aagtataatc tttaaagata tatcaaatag aaagaaggtt ttattagtgg
    44281 cttataaaga tataccaaaa tcggtgcaga aatttatgga agatatcaca caaaaatgtg
    44341 gggatatcca tgcggactgg gcaaagaatt ttacagcagc atttgccaat actctgttga
    44401 caacagtcaa aaagcaaaat gacggaacca cctttttatt gacgggagac attccagcga
    44461 tgtggttaag agactctact gcacaagttc gtccttattt agtgattgca aaagaagatg
    44521 aggaattagc tgacatgatc agcgggttag tcaaaaagca atttttttat atcaatatcg
    44581 atccatatgc gaatgccttc aacgaagaag ctaacggtgc tggccaccaa acagatcata
    44641 caaaaatgaa cgattggatc tgggaaagaa aatatgaaat tgattcttta tgctacccaa
    44701 tacagttggc ctatcttctt tacaaaaata caggaaaaac tgatcaattc aatcaatctt
    44761 ttattgaagg tatcaaaaaa atcttggacg tgttcaaaac agagcaagat catgaacact
    44821 ctccttatac atttgtacga gacacgacaa gaaaagaaga tacgctgatc aatgacgggc
    44881 gaggtgcaaa aacagctttt acaggaatga catggtctgg tttccgtcct agcgatgata
    44941 cttgccgtta tggatatctg gtaccatcca atatgtttgc ggtggtcgtg ttaggttatg
    45001 tggaagaaat ttttcaaacg atactcgatg ataaagaaat tgtacagcaa acacagaaat
    45061 tgaagaaaga gatccaagag gggattcaga atcacggacg aacggttaat caaaaagggc
    45121 aaacgattta tgcatatgaa gtagatggaa tgggaaacgc cacagtcatg gatgacagta
    45181 atgtgcccaa tctgatcgcc gcaccttact tgggatattg ttctgaacaa gaggaagatt
    45241 atctgcaaac aagaagaaca ttgttgagta aagaaaatcc atatttctat gaaggaaagt
    45301 acgcaaaagg aattggatcc tcccatacgc cagaaaaata cgtttggccg attgcattag
    45361 ctatggaagg aatgacaacg tcagacaaat ctgaaaaaga gcggatattg aatttacttg
    45421 tagcaactga cgcaggaaca caccttatgc acgaaggatt cgatgtgaac aatcctgaaa
    45481 attatacgag agaatggttt tcttgggcaa atatgatgtt ttgcgaatta gtcatggatt
    45541 actttgatat tagagtagaa aagtaggagg tatcgtaaat gaaaaagaaa gtatatatca
    45601 tttctcatag ccactgggat cgtgaatggt acatggctta cgaacagcat cacatgcgac
    45661 tgattgaatt gatagacgat ctactagagc tatttgaagt tgatccgtcc ttcaacagtt
    45721 ttcatttgga tgggcaaacg attattttag atgactatct tcaagtaagg ccagaaaaaa
    45781 gacaggcaat ccaacaagca atcaatgaag gaaaattgag aattggacca ttttatatct
    45841 tgcaagatga ttttcttatc agctcggaat cgaacgtgag aaatatgctg attggcatgg
    45901 aagaaagccg taaatgggga acccctgtca tgttaggcta ttttcccgat acatttggaa
    45961 atatgggaca gacgcctcaa ttaatgaaac aagctggtat ttcagcagca gcgtttggcc
    46021 gtggtgtaaa accaattggc tttgataatc aagttttgga agcagaaaac tattcttccc
    46081 aatactcaga gatgtggtgg aaaggacctg atcaaacagc aatctttggt ctgctatttg
    46141 ccaattggta tagcaacggg aatgagattc cagtagaaaa agaagcagct cttgcctttt
    46201 ggaagcaaaa attagcggat gcagaacaat atgcctctac gaaccactta ttgatgatga
    46261 atggtgttga tcatcaaccg gtacaaaaag atatatcaaa agcaatccat ctcgcaaatg
    46321 aactgtttcc agattacgag tttattcatt ctaattttac tgactatctg gaagcagttc
    46381 aaaaagatgt gccagaagac ttaggctcag tagaaggaga attgacaagt caagaaacgg
    46441 atggctggta tactttagcc aatacagcat ctgctcgggt ttacttaaaa caatggaata
    46501 ccaaggtcca gcgacagcta gaaaatatta cagaaccact tgcgacgatg gcttatgaag
    46561 ttagtggaaa ctatccccat gatcaattag attatgcttg gaagacatta atgcaaaatc
    46621 atcctcacga cagtatttgc ggttgttcag tcgattctgt ccatcgggaa atgatacctc
    46681 gttttgaaaa agcagatgaa gttggaaagt atcttgcaca agattcctta gaacagctga
    46741 ccgcagcaat cgatacgacc ggattcccaa aagacagctt tccatttgtc attgtaaata
    46801 ctgcaggaat ggataaaact ggtgaagcag aaataaccat cgaattagaa agaaaaagat
    46861 tcgctgaagg aatacctgaa caactatatc aagagttaga aaatttgcct aaacgtaagt
    46921 atcatgtaga aacaaaaagt ggggccacta taccagctat tttatctgaa gaaactgtcc
    46981 aatttggtta tgatttgccg aaagaccgtt tccgtgtgcc atatatggca agaatgatca
    47041 aagtgacttt accattagaa aacatgccag cattttcttg ggaaacattc gcgcttgtag
    47101 aaggggaagc gaaagcagaa gaaaaggaaa caatgattca tcagagtgga cgaatcatcg
    47161 aaaacggccc tctacatttg acgattgaaa aaaatggtac cattacaatg gaagatcgca
    47221 aaaacaaacg aaagttgaat aatctgcata tttttgaaga tatcggcgat ataggaaatg
    47281 agtatatctt taaacaacct ttctgtgatc agccaatcct atccagtaac aaagaaaact
    47341 ctgaagtaaa agtattagta gatacaccgg aaatagcaaa aataagtatt ctacaggaaa
    47401 tggaaatccc tgtttctgca gacgagcgtt tagaaaaaga acagcagatg gttgtggaat
    47461 ttcgttatcg aaaagcagaa cgatccaaag aaaaacgaat ccttcaaata aaaacaatca
    47521 tgacaatcag aaaagatagt aaaaaaatcg actttgaaac gactctcgac aatcagatga
    47581 aagatcatcg cttacgtgtc ttgtttccta caaagttaca tgtggaacat cacgaagcag
    47641 atagtatttt tgaagtggtg aagcgcccaa accatgtgtc taaaagttgg gaaaacccca
    47701 cgaatccgca acatcagcaa gcatttgtca atattcatga tgaagagtac ggtgtaacag
    47761 tcggaaactt tggtttgaat gaatatgaag taacagaaga cggacaaatc gcagtgacgc
    47821 tacttcgcag tgtgggagaa ctaggcgatt ggggatattt tccaacacca gaagcacagt
    47881 gtctaggtga gcatcggttc aattacagca ttgaactaca tggaccagaa gaaaaattct
    47941 ctacttattt gcatgcatac gcagcacaga ttcctttttc cactcaacaa atcaaacatc
    48001 atgaaggaac attgatttca aaacaacaat atttaacgat aaaaagtgaa acgtttgcaa
    48061 ttacagcttt gaaacgcagc aagtttagtg ataaagtagt agtaagaggt ttcaatatgt
    48121 ctagtcattt agaaaaattg gaaataacta aagacaatgg aaaaactgtc atattgaatc
    48181 tgcttgaaga accaacgaag caagcggttg tcccaataat ccaaccttat gagattcgga
    48241 caatcggatt cgaggaggaa aacgaatgta tggcggaata gaagcaggcg gaacaaaatt
    48301 tgtgtgtgcg gtttcagatg gtggagaaat tttagaaaaa ttaagtatcc ctaccacaac
    48361 accagaagaa acattggatc aggtatttga attttttgat cgttatgaat tgaagggaat
    48421 cgggattggc tcattcggcc cgattggtat cgacaaaaca aaagataatt acggatacgt
    48481 tttggcaact cccaaaaaag ggtgggcaca attcgatttt cttggcacaa tcaaggaacg
    48541 ctacaatatt cctgctgctt ggacaacgga tgtcaatgct gctgcatatg gggaattgaa
    48601 gcaaggagca gcacaaggaa aaaatagttg tatttacctt accgtgggaa caggaatcgg
    48661 tgcaggtgtc gtgatcaatg aagaaatttt ttcagggatc gcacatcctg aaatgggaca
    48721 tatttgggtg aaacgccatc cagatgacca ttatgaaggt acttgtcctt atcataaaga
    48781 ttgtttagaa ggattagcag caggaccgtc tattgaggca cgcacaggga tcaaagggca
    48841 agatctgccg caagatcatc ctgtttgggc tattcaggct tattatattg ctcaagcact
    48901 gatcaactat acgttgacgt tagcacctga aaagattatt ttaggcggtg gggtaatgaa
    48961 tcaggatcat ctccttcaaa aaatccgtca gcaatttgtt gaattgatgg gtggttatat
    49021 ggaaacacct caagtaagtg aatatatcgt tcgttgggga ctgccgaatg aaagtgggat
    49081 tatcggtagt ttgctgttag cagaacacga acatcaaaag taagcagaca aattacacaa
    49141 aaagaacaga gtcttatcat tatctgctga taagactctg ttctttttta tttctattcc
    49201 cataatccag gtaaagcttt accgactatt ttttcttgag taaatggatg gatcatgtgc
    49261 agttctaatg catggagcat cagtcgttca gctgttctat tttggtagag agggtctcca
    49321 acgatcggat gtcccttatg agctaaatga actctgatct ggtgtgtacg tccggtttcc
    49381 aaaacacaat agacttgtgt ttgttcagaa agtactttgt tgacagtgac atgtgtcaag
    49441 gcgctctgac catttttatc atctacgacc ctttttctcc gatcatggcg atctcgtcca
    49501 attttattgg tgatggtcat atctttggca atttctcccc atacgatggc ttgatatctc
    49561 cgatagatca ttttttgttc caacattctt ccaagtatcg gcaagacaaa tggatttttt
    49621 gcaaataaaa cggcaccgct tgtttctttg tccaagcggt ggacaacgta aggtcgttgc
    49681 tgtttagtgt cgagataagc agctaaatga ttgagtaagg tactgttttc tgtcggttca
    49741 tttggatgag tcttcattcc tgcaggctta tccactataa tcaaatgctc atcttcatat
    49801 aaaacacgga tgtgcttcgc atctcctaat tgaacttctt gataattata atctgtttct
    49861 tcgaataaca atgtgatctg gtcattgccc tttacttcat gatggaacaa agcaggttgt
    49921 tgattgatcc aaacgttttt acgtatacgc aaaaagtgtc gaacctttct aggaactaac
    49981 cattcctttt caagcaagtc gcgaattgtt gtttcaggat ggttcatagg taaagtgatc
    50041 gtgattttca caagtgtact cctttcagtg caatcgcaga ctaattgtac catttttaaa
    50101 acatatttaa gaaaaaactt acatttttct gttcattctt atgttactct acttgcacta
    50161 acgtaaaaaa acatattgaa ctacaactta agcaggaaga aaacactctt tcactatggt
    50221 aaaataagac gattgaatca aatagacagg agaagaagca aatggatttt caaagcattt
    50281 tagaaaagat aaaagctgct ttgatcagtt tttggcgttg gatcagacca tatctcggac
    50341 aattccatcg atggcgaaag cgcatctgga aaaaatatca tgtgaacaaa ttgattttgc
    50401 tgattggttt gatctgtgtc ttagtgacaa gtatctacct ctttatttta gcaaaacaag
    50461 caaatgtcga aacgttaaaa tcaggattaa gccaatcgac agtcatttat gataagaaaa
    50521 acgaagaagc tggcacactg tacgcccaaa aaggaacata tgtagaatta aacgcgattt
    50581 ctccctatat ccaagatgca gtaatctcga cagaagatag gaatttttac gagcatcacg
    50641 ggtttgatat caaagggatt gcccgtgcag cagtccgaat ggtcttgtct ggcggaacat
    50701 cagggggcgg aggaagtacg atcactcagc agcttgccaa aaatgcgtat ctgacattag
    50761 accaaacgtt tgatcgaaaa gcgaaggaat tatttttggc aatcgagatc gaaaagaagt
    50821 acagcaaaga agaaatcttg accatgtatc ttaacaatgc ctattttggc aacggggtat
    50881 ggggtgtcca agacgcttcc agaaagtatt ttggagtaga tgcaacgaat gttacgctgt
    50941 ctgaggctgc tactttggca ggaatgctga aaggtcctgg tatttataat ccgatcgatc
    51001 acccaaaaaa tgcgaatgat cggaaaaaca cagtactaag tgtaatggaa gaaaacggaa
    51061 aaatcacaaa agaacaagaa caagcagaaa gccagacaga tatcagcgcg tatcttaatg
    51121 acacatacga taattcagat tctggttatc gttatcctta ttattttgat gcagtgatcg
    51181 atgaagcagt taacgagtat ggtcttgatg aagaagatgt catgaataaa ggctataaaa
    51241 tctatacagc tttagatcag gattatcaaa agcaattaga agctacttat caaaacagtg
    51301 cattattccc agcaaatgct tcagatggtg ccatggtcca atctgcttca gtagctttag
    51361 atccgaatac aggtggtgta caagcactag taggacgaag aggagatcat gtctttagag
    51421 gattcagctt tgctacacag atgcgtcgtt caccaggatc aacgatcaaa ccattaagcg
    51481 tatatacacc agcactggaa gctggataca agccttccag tatcttgaaa gacgaaccgc
    51541 aaagttatta cgatgcgcat aattttgacg ggacatacca aggagaagta ccgatgtatc
    51601 aagctgttgc acaaagtttg aatttgccta ctgtatggct tttgcatgag atcggtttac
    51661 aaaaaggata tgataaagca gaagaattcg gcctgccttt agcaaaatct gataagtatt
    51721 atggcttagc ccttggtgga ctggaaaaag gagtatctcc attgatcatg gcgggtgctt
    51781 acagtgcatt tgccaatgat ggtcagatgt atacgccgca tcttatcacg aaaatcgttg
    51841 attcgactgg agcggtgatc gtcgataaca caaacaaaaa accaaaacag gtcatatcaa
    51901 aggaaacagc cgatgaaatg accagcatgc ttcttggtac attttcaaat ggaacagcgg
    51961 ctgctgcgaa tcctgcaggt tatacgatag cagggaaaac aggaaccaca gaaactaatt
    52021 tcgatgccac aaaagcaaat gaccaatgga tgatcggtta cacgccagac gtagtgattt
    52081 cgacatggat gggttacgaa gaatccagca agctgcacta tttggaaggg acaagtggaa
    52141 atgttgtcgg gaaagtcttc aaatcagcgg cagaaggcat ccttccgtat acttcaaaaa
    52201 caggatttag tgtagcagat gcctatgcca caggaggaca agtggtagct gcagatcaag
    52261 ttggaaacac aggaaattct aatggagagt ctggaaattg gcaagataac ttgaatcaat
    52321 atggtgaaca agcaaaagaa gggctgaaaa acttcggcga tatggtgaaa gaaggcgttc
    52381 agggaattgg tgaagcggct aaagacctct ggcggaaata tcaaggagag tagccccttt
    52441 gaaaacgaat aaccccatgt tacaatagag agtaactact aaaagaaaag agggcataac
    52501 atgtcaaaca tctacgatag cgcgaatcaa atcgaacgtg agatccgtga attaccagaa
    52561 ttcaaagcgt tgaaagaagc gtacgagaaa gtacaagcaa acgaagaagc acacaaactg
    52621 ttcaaagact tccaagcgat gcaaatcgaa ttacaagaaa aacaaatgag tggacaagag
    52681 ttcagcgacg aagatgcaat gaaagcacaa gaaatggctg tgaaaatcca atcagaagaa
    52741 gtaatcaatg acttgatgca aaaagagcaa ggcttcagta caatcatcaa tgacttgaac
    52801 cgtatcatca tgacaccagt tcgcgacctt tacagcgaat aaaactattt tatgaaacaa
    52861 cagagggtgt gaaagagacg ttcggccttg agcgatgaca aaattgcttc ttattgccga
    52921 taggctgtct cttgaacacc atttaccaag aggtcgtgag cctaccgttt aactctgagt
    52981 tacaaggaac aatttcaaaa aacagcttct catgtttgat gactattgtg acttgtgacc
    53041 gaggagttgg caggagaaca ccgtttattc agaaatagga gctacgacaa aagtcttgtc
    53101 atagctccta ttttttgtca ttcatttttc aagcggtaga aatagccttt ttgagctaac
    53161 agtttttcgt aagtacctat ttcaacgatt tttccttcat cgatcacaac gattttatct
    53221 gcctctttga tcgttgaaag ccgatgagcc acaataaaag tagtgggtgt ttctgtcagg
    53281 taattcaggg cttgctggat ttttttctct gactggttat ctaaagctga ggttgcttca
    53341 tctagcaaaa tgatttttgg ctgacgtatc aacgctcgtg caatggagat acgttgcttt
    53401 tggccaccag acagcttgtt gccatgttcg cctactaagg tatccagacc atcaggaagc
    53461 tggtcaatca gtgaagaaag ttgcgcagct tcgatgactt catctaaacg ttcttttgat
    53521 acgtttttca agccataagt gatgttctct ttgatcgaag cggagaacaa caaagtggtt
    53581 tgaggaacaa cggctaaata atttcgcaca cttcgcatat ccagttcatc caaaggctga
    53641 tcatctagaa aaatttttcc tgaagtcggc tggataaatc caatcaataa gttaaggagt
    53701 gtagattttc cagagccaga tggcccaaca aaagcaattg tttctccagc tttcacctct
    53761 agagaaaaat gatcgataat cggtttgagt gagtccggat aaagaaaaga aacatcctca
    53821 aaacgaaaat ttccatccaa atgttggatt tttttcttac cgtgataagt ttctacatcg
    53881 ccagcaatca acagttcgcc aatagaagtg atagattcca tgccttttga aatcgtagga
    53941 atcagcgtga tcattccaga aacggaattg acgatcgtcg tgaaatacgt ctgatacatc
    54001 acgacatcgc cgggttggat gcgcccttgt aaagctaata tcccagtgaa aatcaaacaa
    54061 atcagctgaa atatctgaaa actagcccag ctgactgatc cgaaaacaga ttgcaggata
    54121 tctaagtgga atcctttttc atagatttga gtaaaatgct tatttaagcg agttgtttcg
    54181 tgtttttcta acccatgagc tcgagcaatc ggtgctaatt cgatcacttc gatcactttt
    54241 gcagaagtct cttccatttc ttgcctgaag tctgagttgg actgtctgat ccttttacgg
    54301 aaaaagagag taacgaatgc ggcgagagga acagtcaata agaaaaacat gaaaacaaca
    54361 ggacttttat aaagcgtgat acctaaagct acgaataaat tgacagcgat atttaatccg
    54421 ctgacaaata cttgagagga caacgtttgg atagcttcta catcacggat gatctttgac
    54481 tggattcgtc cagattgcaa ttctttttga taaggaatag atagttcttg gattttcttg
    54541 ataagggtcg ctcttagtcc agcttctact tgacggatcg aagtgctatg gtattttaca
    54601 tgaaaataat tcgtcaatat attttgacca atcagacaag ccatcaaaaa agcgttgaaa
    54661 taaatggcct gcaactgaga agaatctttc gatgaagctg cattgattac attggcaata
    54721 gcgatcggca ttaaccaaga tggaagatgt ttgataataa aaaagagtcc tgacatggca
    54781 aaacgccaat aatttccctg ataaagatgc agcaatgtct gaaaagcatt tgcatgatat
    54841 ttttgataac ttttctgtat tcgttcctcc ataatcttct ccttaacaat cttggtaaat
    54901 aaactgcaag tgttctattg cttcaggaac ataaatctca ggagtagctt caagagtggc
    54961 gtacattaga ggacgctcat atttgatata atgcagtacc gggcggaaat ctaaacagcc
    55021 cttgccaaca ggtaccatgc ggatctttcc attttccatg atatagtctt tgagatgaag
    55081 cgctgtaata taaccatgta aattgtccaa ggcacctcgg atcacttttt cctgatcagc
    55141 atgattttct ttatgcatca aattcgcaca atctaaaatg atttttaaat ttggtgaagc
    55201 tacttcatcg attaaacgtt tagctaactg atagttatag attggatgat tgatccctgc
    55261 ttcaacagca acagttacgc caaaattttc tgcatgagcg actaattcaa tgatagaatt
    55321 tctggcgatc tgataagctt cttcagtaaa attttctttt gtgtaaccga cagagacact
    55381 accggtttcc gtagcaatca tcttggcttg ataggcagag cacagagcaa tttgctgttt
    55441 gaaagttgcc aatgcttgtg cacgaacttc ttgatttttg ctggataggt tgatatagca
    55501 tcctaaaatg gaaagttcga tattgttttt ttggaagtat tcaccaaaat aggcagcact
    55561 cccaagtgtc atgtcagtta atttaagttg ttcaggaaat gctttttgtg gtgcaaattg
    55621 gatatgggac aagtgaagct gtgatacttt ttgaacgatt tcttcacgat ttcgaacatc
    55681 taaatcgtgg gcacggatgc ctaaatttaa tggaaacatc tcaaactcct ttttgtaaat
    55741 aattagcaag aggcagacgg gattctttta tcaattcaac aattattcgg gcaactttat
    55801 cagcgccttc tggactaaag tgggtattgt cttttagtcc ttctgggtaa tttttacaag
    55861 tatcaggtat aagatgcagg tagaattttt ttgtctcttc ttttgagaag gtctggaaca
    55921 attcttgtga tcttgagaac acatctaaaa gaggaatatg gtgtttctca gcaaatgcac
    55981 gcatcgcttt tggatagtct ccaagtatat ctggattcaa tgttccgttt tctaagtaat
    56041 ctcgtcgcgt cacagatgtc agtaagacca tttggacgtt cgcttttttt gcgcattcaa
    56101 aaaatatctg cagatttttt aaatagctac cgaagggaag cgtacctctt tcttcttgga
    56161 ttttctggtc gttatgtcca aattgtacaa ataagaaatc attcggctga aactgtgaag
    56221 ctagacgggc taaccttcct tcctggataa atgatttggt gctgcgtcca ttctttgctt
    56281 ggttattgat ctggatctcg ggtgaaaaat aagcagagat tttttctccc caacctgtct
    56341 cgggacgctt gttttcatct ttttctgccg cagtagaatc accggcaata tggatagtag
    56401 gcatgttatc tcttctttca taaaggttta taggcaatga agcagaacag caagagaaga
    56461 agacggagca aatttggtcc gtcttcttct ctaaaacgtg gataaacgga ttccgttatt
    56521 tcactatcct gtttttgatt cgttactgtt actattaatc aattaggcaa aaaagccaaa
    56581 gtaattttta gcatttgtat aactaatcgc ttgaactaga cttcctaaaa gttgttcatc
    56641 ttcaggtgct tctcctcgca tgaccatttc gcctaaaaat tcacaaagaa cacgacggaa
    56701 atattcatgt ctagtgtagg acaggaaact tcttgaatct gtcagcatgc cgacaaaatt
    56761 ccccaaagca ctaccatcag caaattgtgt caattgatcg cgcataccag ctcgagtatc
    56821 gttgtaccac catccagaac ctaactgcag ttttccattc tgttcttttt ggaaacagcc
    56881 catcaaagct aaaatcgcag gatagtcatt cggattcaat gaataaagga tcgtctttgg
    56941 caactgatcc gtttcttctg cacggttcaa taatttttgc agaggaatag tcaaagaaag
    57001 atcattcatt ccatcatagc cggtatcggg ccctaatttt tccaacattt gtgtattaca
    57061 gttacgataa gcatggatgt gcagctgcat cgtccaatta tgagaatgat agaagtgggt
    57121 caaatgtatc agtgtttctg tacgataagc atcgatttca tctgaagata atagtttgtt
    57181 agctcgtgct ttttgaaaaa tctcttctaa tactgtttca tccgcttctt tatagcgtaa
    57241 gatgtctaaa gcgtggtcgg aaacacgaca tccatgttga tggaagtatt ctatccgctg
    57301 ccctaagact tctatcagtt cttgataggt ggtgatcgtt tggtcagctg cttcttccag
    57361 tagtgtgacc catgcattaa atccttcttt ttcaatattg agtgctttat ctggacggaa
    57421 tgaaggtaga accttcaaat ctggttcttc atgagcaagc aactgatgat agtgcaagtc
    57481 gtctgcggga tcatctgttg tacaaatcac ttctacattc gatttttttg cgatttcccg
    57541 acggcgaaaa tcaggagctg ccaaaatttc atttgcttgt gtccaaattt tctctgcatt
    57601 ttcttcattt atcaacaagt caataccgaa gaaacgcttc aattcaagat gtgaccaact
    57661 gtaaagcgga tttccgataa tttttggtac ggtttgacac caagctaaaa atttttcaaa
    57721 atcagaagca tttcctgtga ttttttcttc aggcacgccg caagctcgca tcaagcgcca
    57781 tttgtaatga tcgcctccta accaagcttc tgtcagattg ctgaaattgt tgttttcgta
    57841 aatttctttt ggatctaagt gacagtgata atcaataatg ggcatgtttt ttgcgtagga
    57901 atggtaaagc tttttcgccc aatcgtcttt taataaaaaa tcatcagata aaaacataaa
    57961 tcttccttct ttctttttat actgttttat gaaaatcaaa gtgttactcc gtttttaaaa
    58021 atggcaattt ctcgaacgtc attttgttca tttttcgttt cttctccact agctacagcg
    58081 atgatttttt gaacaaacga ttctaagatt acttccattg gttgttctaa taagacgcct
    58141 gcattaaaat ccatccaatg acctttgcgt tcaaaaagag ttgtattcgt agataccttg
    58201 accgttggga catatgagcc aaacggagtc cctcttcctg tggtaaacaa gaccaactgg
    58261 caatcggatg aagcaagagc agaggcagct acaagatcat tcccgggagc ttgcaataaa
    58321 ctaagccctg gttttctgat tttttcgcca tattgtagca catcaacgac aggagagctt
    58381 cctgattttt gcgtgcagcc taaagactta tcttctaatg tagtgatccc acctgcttta
    58441 ttaccaggag atgggttttc gtaaactggt tccccgtaag acaagaaata ctgtttgaag
    58501 tcattgatca agtcaacgat tgagtcaaaa acttcttcat tttccgctcg tgccatcaat
    58561 acttgttctg cgccaaacat ttctggcact tctgttaaga tcgtgctgcc tccttgagca
    58621 atcaagtagt cagaaaatgc accaagtaaa ggattagcag ttattcccga tagaccatca
    58681 gaaccgccgc atttcaaacc gacattcaac ttagagagcg ggactgatat gcgctggtca
    58741 ttttccgccg ctgttaaaag ctcttctaat agagccacac cttgttcgat ctcatcgtaa
    58801 acatcttgag ccactagaaa tttcactcgt tcttcatcat attcacctaa aatttttttg
    58861 aattcaggaa ctgtattgtt ctcacaacct aatccaaata ctaaaactcc tccggcattg
    58921 ggatgattta ctgcgtccgc taaaatttct cgagtatttt gatgatcttt tccaagctgc
    58981 gaacagccat aaggatgctt taaaatcgta atatggtcaa actgtcccag atctggatgt
    59041 ttttccttga actgtttgac gatcagttca gcaatgccat tcacacaccc aacggtaggt
    59101 actaaaaaca aatcattacg aatccctact tttccatttt ttcgcaagta accttgaaat
    59161 gttcggttct caaaaaggta atgacttgga tggacatctt tttcatatcg ataatccaat
    59221 tcaccagaaa gattggtttt tacattatga gtgtgcagcc attgaccggc agttacgtct
    59281 gtcgtcacat gaccgattgg atagccatat ttgatcacgt tgtctccagc tttcaaatca
    59341 cccaatgcaa tcttatgacc ttgcgggata tcttcaagag tggtgatcgt ttgctgatca
    59401 atcgtcagtt gtgtttgttt tggaataggg gttaatgcga cgaccacact gtctcgtcga
    59461 tttaatttca ttgttggttg cagggtttca agcatggacc atcgttcctt tcatttcttg
    59521 taatactgac cggacacctt tttgatcgat ctttgcagct atttcttgaa ctgtatctgt
    59581 cacattcggt atttgagaca aatcttctcc ccaaagcgca gcgttttgca ataatcgagg
    59641 aatagctgtt ccattatcag accaagcatc agtaaattcg cttatcgtct tttcatcatc
    59701 atgtggcttt acttgatcgc ctcgatacat aagaagtagc gcagctagtg aaacagtgat
    59761 taacggtggt acctgatttt gttcttcgat atacttcttg aacactggaa gcagacgggt
    59821 gctgaacttt gaaacactat ttaatgaaat cgcttctaat ttgtgatggg caaaaggatt
    59881 caagaaacgt tctttgattt ggtcagcata aatggctaat tcatctttag gtaaagaaag
    59941 cataggaatc aattctagat tgaataactg atcaacatat gcggagaata aaggatcttt
    60001 cattacttct tctacagttt ccaattccgc caacaaagca agagggacca tcgctgtatg
    60061 cggtccatta agcaaatgca ccttacgttc acgataagga gtcatatcat ctgtgatgat
    60121 gacattcaag ccagcctgtt tgaatggtag ttgattctcc aattctttag gcccttcgat
    60181 cacccaaagc atgaatggtt ctgctttgac catcaattga tcttcatagt tatgccgttg
    60241 attgaaaaca cctgcatctt cgcgaggata acctgggacg atgcgatcaa ctaaactaca
    60301 acagaaaaca ttttcttttt ctaaccactc aacaaaagca ggttctaaat cccatttttc
    60361 tgcgtagcgt aaaatgattt cttttagacg ttcgccgttt ctgtcaatca gttcgcaagg
    60421 aatgatggta aagccagctt tgtttaattt gaaacgtcgg tataaaaagg ctgtcagttt
    60481 gcccgggagg ctctgtgggg gagtatctgt taaattatca gtctccacga attggattcc
    60541 tgcctctgtt gtattggaga cgatataggc caagtcgtca ttttctgcta gagatagata
    60601 agcatcccat tcatcataag gattgatcat ttgttctact acggtgataa tctcggaagt
    60661 atcaacgatt tcgccattgc tgagtccttg taaaatgacc gtatataaat tatcttgctg
    60721 gttcatcata tttccaagac cttgttctaa aggttgaatg attgcaacat tacctttaaa
    60781 caattgttgg ttattcagct gctgaatctg ccaatcaata aatccacgca taaaattgcc
    60841 ttcaccaaac tgaagaactt ttacaggtgc ttcgtttttt ttcactaatt cttttgtcag
    60901 ttttttcata taaacttcct ttctgtgtta tgcacacgtt aacattttta tataaaatta
    60961 cttgtagttt acaagtaatt gatcgactca cgaaggatca acaaggtgga gagctcaatt
    61021 ttagattgtt cgatcgtcct gccttcgatc aaatctagta aaagctttgt tccttcttga
    61081 cacatttctt tcaaaggctt acgtacggtg gtaagcggcg gattgagata ggagacgact
    61141 ggagaatcat cgaacccgat cagagaaatg ttctggggaa cacggaggcc agctaaatgt
    61201 acagctctca atgcaccaat cgctatgtcg tcgttgccgc aaaaaaccag agtaggcagg
    61261 tcagaaagtg ataataggta ttccatgttt tggaggccgc tttctaagct gtaatctcct
    61321 gttaaaaaat aggctggatt gacagggatg ctcgcttttt ttagactttt caatacccct
    61381 ttttcccgtt cttttgtcga acgaaaattt ttctttccgc caatataagc gatcttcgta
    61441 tgtccgcatc caataccgta atctactgcc tgcataacac cggccgcatc atcggcagtg
    61501 acgttgataa ccgctggatc attgatgtga cgattgacaa caacgaaagg aaattctttt
    61561 tctttgagat agtcgatgaa aggctgatca tcatcacttt gactcataac gatcagtcca
    61621 tcgtagcgtt gcaggttgat ttctgttaaa tcaacaacat cgatactttc aacagataaa
    61681 ctgtaatttg tgtctaatac tgaatggatg cctgcaatgg aatctgctag gaaatttgat
    61741 gaagttcctt tatgcaaact tgagaaaaat agtccgatta gatatttttt ttgattcact
    61801 aaaccttttg cactatagtt tggcgaataa ttcatttcct tagctagttg tcgtattttt
    61861 tgttttgttt cattttttat taaactgctg ttattcaaag cacgagaaac tgtcgtatga
    61921 gaaacgccag ctgctttagc gacatctttg atcgttacca ttccatcatt ttccttactt
    61981 tttcactttt tgttaacgca ttcatcatat tacgataact acgataagtc aatagatctt
    62041 tgatatgaaa agattgcaca cgttaaaaat tacttggatt tttattttta attatgctat
    62101 tcttgaaagc ggggaatgct catttaatta gatgagtatc aattttacag aaaaatgaaa
    62161 gccttttatt ttaaaaggag gagaggaaga aaatgaagaa aagtacagta ttttataaaa
    62221 tatttttacc gatgttttta ttaggagccg tacttgtcat aggatttagt ttattcattt
    62281 accagaatac ctatgaatcg atcgaatcga gttatttaac agacaaaaa
//
LOCUS       DACDNO010000009        58316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDNO010000009 DACDNO010000000
VERSION     DACDNO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.009"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            245..583
                     /locus_tag="IWI22_000585"
     CDS             245..583
                     /locus_tag="IWI22_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168003.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
     gene            934..3090
                     /locus_tag="IWI22_000586"
     CDS             934..3090
                     /locus_tag="IWI22_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168004.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAEDVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEDATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIIAVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            3102..3335
                     /locus_tag="IWI22_000587"
     CDS             3102..3335
                     /locus_tag="IWI22_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9168005.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            3325..4191
                     /locus_tag="IWI22_000588"
     CDS             3325..4191
                     /locus_tag="IWI22_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168006.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            4206..6176
                     /locus_tag="IWI22_000589"
     CDS             4206..6176
                     /locus_tag="IWI22_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168007.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRLEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAEINVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            6343..6570
                     /locus_tag="IWI22_000590"
     CDS             6343..6570
                     /locus_tag="IWI22_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168008.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            6567..7061
                     /locus_tag="IWI22_000591"
     CDS             6567..7061
                     /locus_tag="IWI22_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168009.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFEL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            7236..7571
                     /locus_tag="IWI22_000592"
     CDS             7236..7571
                     /locus_tag="IWI22_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168010.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(7828..9411)
                     /locus_tag="IWI22_000593"
     CDS             complement(7828..9411)
                     /locus_tag="IWI22_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168011.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALV
                     LDLIDKLSDLANIYEDITSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLPYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLKILKEEISKYRFTAESREEINKSLGENQQITYTK
                     GVFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGSN
                     NEELQNHLNSLLDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKNLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            9533..10117
                     /locus_tag="IWI22_000594"
     CDS             9533..10117
                     /locus_tag="IWI22_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168012.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSIT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIDTSIKELREYVQNPYNFQYLAGRINLV"
     gene            10128..10373
                     /locus_tag="IWI22_000595"
     CDS             10128..10373
                     /locus_tag="IWI22_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168013.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLVLNKRKKDR"
     gene            10379..11101
                     /locus_tag="IWI22_000596"
     CDS             10379..11101
                     /locus_tag="IWI22_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168014.1"
                     /translation="MKSTKWKIVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            11232..11975
                     /locus_tag="IWI22_000597"
     CDS             11232..11975
                     /locus_tag="IWI22_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168015.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            11981..12346
                     /locus_tag="IWI22_000598"
     CDS             11981..12346
                     /locus_tag="IWI22_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168016.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            12362..12892
                     /locus_tag="IWI22_000599"
     CDS             12362..12892
                     /locus_tag="IWI22_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168017.1"
                     /translation="MSQNIGAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            13054..13422
                     /locus_tag="IWI22_000600"
     CDS             13054..13422
                     /locus_tag="IWI22_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168018.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            13613..13873
                     /locus_tag="IWI22_000601"
     CDS             13613..13873
                     /locus_tag="IWI22_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168019.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            14491..15291
                     /locus_tag="IWI22_000602"
     CDS             14491..15291
                     /locus_tag="IWI22_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168020.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            15328..15510
                     /locus_tag="IWI22_000603"
     CDS             15328..15510
                     /locus_tag="IWI22_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168021.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            15700..15987
                     /locus_tag="IWI22_000604"
     CDS             15700..15987
                     /locus_tag="IWI22_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168022.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            16078..16323
                     /locus_tag="IWI22_000605"
     CDS             16078..16323
                     /locus_tag="IWI22_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168023.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRNRMFSYYESMRCLNKSRSEI
                     IESSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            16451..16780
                     /locus_tag="IWI22_000606"
     CDS             16451..16780
                     /locus_tag="IWI22_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168024.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            16833..17664
                     /locus_tag="IWI22_000607"
                     /pseudo
     CDS             16833..17664
                     /locus_tag="IWI22_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17679..17996
                     /locus_tag="IWI22_000608"
     CDS             17679..17996
                     /locus_tag="IWI22_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168025.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            17999..18231
                     /locus_tag="IWI22_000609"
                     /pseudo
     CDS             17999..18231
                     /locus_tag="IWI22_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18346..18822)
                     /locus_tag="IWI22_000610"
     CDS             complement(18346..18822)
                     /locus_tag="IWI22_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168026.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            18948..20279
                     /locus_tag="IWI22_000611"
     CDS             18948..20279
                     /locus_tag="IWI22_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9168027.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            20304..20831
                     /locus_tag="IWI22_000612"
     CDS             20304..20831
                     /locus_tag="IWI22_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168028.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            20818..21381
                     /locus_tag="IWI22_000613"
     CDS             20818..21381
                     /locus_tag="IWI22_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168029.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            21799..22536
                     /locus_tag="IWI22_000614"
     CDS             21799..22536
                     /locus_tag="IWI22_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168030.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(22553..22969)
                     /locus_tag="IWI22_000615"
     CDS             complement(22553..22969)
                     /locus_tag="IWI22_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168031.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            23362..24609
                     /locus_tag="IWI22_000616"
     CDS             23362..24609
                     /locus_tag="IWI22_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168032.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            24879..27269
                     /locus_tag="IWI22_000617"
     CDS             24879..27269
                     /locus_tag="IWI22_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP9168033.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            27318..29153
                     /locus_tag="IWI22_000618"
     CDS             27318..29153
                     /locus_tag="IWI22_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9168034.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            29228..30085
                     /locus_tag="IWI22_000619"
     CDS             29228..30085
                     /locus_tag="IWI22_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9168035.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            30075..31154
                     /locus_tag="IWI22_000620"
     CDS             30075..31154
                     /locus_tag="IWI22_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9168036.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSEYVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLNVLILLIKEFRKA"
     gene            31493..32293
                     /locus_tag="IWI22_000621"
     CDS             31493..32293
                     /locus_tag="IWI22_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168037.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            32381..33187
                     /locus_tag="IWI22_000622"
     CDS             32381..33187
                     /locus_tag="IWI22_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168038.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTAENWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            33418..34287
                     /locus_tag="IWI22_000623"
     CDS             33418..34287
                     /locus_tag="IWI22_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP9168039.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGNVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            34307..34633
                     /locus_tag="IWI22_000624"
     CDS             34307..34633
                     /locus_tag="IWI22_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168040.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDD"
     gene            34667..35929
                     /locus_tag="IWI22_000625"
     CDS             34667..35929
                     /locus_tag="IWI22_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168041.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVANFSKSVYLVGRALVVSAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            36009..37385
                     /locus_tag="IWI22_000626"
     CDS             36009..37385
                     /locus_tag="IWI22_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9168042.1"
                     /translation="MAMKLLEKVKNRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISDTPHMLVAGTTGSGKSVLLNEILVTAICHAMPDELKLG
                     IVDPKRVEFGRYKKLPYMLADPITDMDEAYEFFEYLVILMNERYKLMEKAGVQNITQY
                     NKYAEKHGIEKFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMFIKLNGGNKIRAQG
                     AYISNEEIEAIFDHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     VDPEPQTSNNTNNEQPISSKTKPKTTDPKVSVKVTITTDNSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            37382..38011
                     /locus_tag="IWI22_000627"
     CDS             37382..38011
                     /locus_tag="IWI22_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168043.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNERKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            38023..38481
                     /locus_tag="IWI22_000628"
     CDS             38023..38481
                     /locus_tag="IWI22_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168044.1"
                     /translation="MLTILKLYREGFNEKVSIGESIEYRGDKYIIIRILKINKHYFSK
                     SISLTVRVLVQKVGDLSDYYNCKREVIVDNRYDQSKDDKRIIKIGDIVFPEKGIAYEV
                     VSINSFRYEFVDLVVEYSVRLIVPWSKEEINEALKDERKSTFKVLEGEKK"
     gene            38478..38957
                     /locus_tag="IWI22_000629"
     CDS             38478..38957
                     /locus_tag="IWI22_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168045.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGRERVDYITYS
                     VDNTVRCYEIKVTLSDLKSKAKQTFIGDYNYLVVTSDLWEKMLQDDTLMRKYYKQGVY
                     VFNEKYPSMISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIVEIPE"
     gene            39096..39635
                     /locus_tag="IWI22_000630"
     CDS             39096..39635
                     /locus_tag="IWI22_000630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168046.1"
                     /translation="MNKRRILWLIAGMYLCIAVSGFTICRIINQLKNAIVYLGEIGYL
                     EDLKAGLQIMSWILFVLTVALVILTCENFSTIKKQIISNHNQETVKSGLEVDPEEEVT
                     EQAIKEQEEIDKKEIMSIYLSSGESFFHEIDSLKLSSQEIIDRWQNMSLDGELIVEDS
                     EGWSAYKKDEIVAITILFV"
     gene            40286..40339
                     /locus_tag="IWI22_000631"
                     /pseudo
     CDS             40286..40339
                     /locus_tag="IWI22_000631"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            40343..40942
                     /locus_tag="IWI22_000632"
     CDS             40343..40942
                     /locus_tag="IWI22_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9168047.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            40958..41434
                     /locus_tag="IWI22_000633"
     CDS             40958..41434
                     /locus_tag="IWI22_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168048.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMHE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            41451..42353
                     /locus_tag="IWI22_000634"
     CDS             41451..42353
                     /locus_tag="IWI22_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168049.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            42426..42617
                     /locus_tag="IWI22_000635"
     CDS             42426..42617
                     /locus_tag="IWI22_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168050.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            42782..43369
                     /locus_tag="IWI22_000636"
     CDS             42782..43369
                     /locus_tag="IWI22_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168051.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            43369..44268
                     /locus_tag="IWI22_000637"
     CDS             43369..44268
                     /locus_tag="IWI22_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP9168052.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            44268..45182
                     /locus_tag="IWI22_000638"
     CDS             44268..45182
                     /locus_tag="IWI22_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168053.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            45234..45449
                     /locus_tag="IWI22_000639"
     CDS             45234..45449
                     /locus_tag="IWI22_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168054.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKKFWLNNFSYKDFIKEKANTLY"
     gene            45639..45893
                     /locus_tag="IWI22_000640"
     CDS             45639..45893
                     /locus_tag="IWI22_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAP9168055.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            46072..47115
                     /locus_tag="IWI22_000641"
     CDS             46072..47115
                     /locus_tag="IWI22_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP9168056.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            47288..47512
                     /locus_tag="IWI22_000642"
     CDS             47288..47512
                     /locus_tag="IWI22_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168057.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            47515..47772
                     /locus_tag="IWI22_000643"
     CDS             47515..47772
                     /locus_tag="IWI22_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168058.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            47874..48227
                     /locus_tag="IWI22_000644"
     CDS             47874..48227
                     /locus_tag="IWI22_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168059.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            48280..49074
                     /locus_tag="IWI22_000645"
     CDS             48280..49074
                     /locus_tag="IWI22_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168060.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            49103..50221
                     /locus_tag="IWI22_000646"
     CDS             49103..50221
                     /locus_tag="IWI22_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168061.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            50254..50574
                     /locus_tag="IWI22_000647"
     CDS             50254..50574
                     /locus_tag="IWI22_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168062.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            50829..51722
                     /locus_tag="IWI22_000648"
     CDS             50829..51722
                     /locus_tag="IWI22_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168063.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            51742..53370
                     /locus_tag="IWI22_000649"
     CDS             51742..53370
                     /locus_tag="IWI22_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168064.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKEFEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            53421..53789
                     /locus_tag="IWI22_000650"
                     /pseudo
     CDS             53421..53789
                     /locus_tag="IWI22_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            53889..54089
                     /locus_tag="IWI22_000651"
     CDS             53889..54089
                     /locus_tag="IWI22_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168065.1"
                     /translation="MLKRIAWDKDNKTVIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            54223..55449
                     /locus_tag="IWI22_000652"
     CDS             54223..55449
                     /locus_tag="IWI22_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168066.1"
                     /translation="MIFYMFININEVLKMTTITMTKNIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYRNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRLKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            56374..57699
                     /locus_tag="IWI22_000653"
     CDS             56374..57699
                     /locus_tag="IWI22_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9168067.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRANDLPYPLPKELYIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSETRSERRRELAKIIKDEIEKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            57692..58039
                     /locus_tag="IWI22_000654"
     CDS             57692..58039
                     /locus_tag="IWI22_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168068.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
BASE COUNT        22212 a         7690 c        11510 g        16904 t
ORIGIN      
        1 ctggtaaaga acagaattat tatctaaaca aaaacttgat aaaccagcat ttttagccaa
       61 taaaaaaagt gaaataattt aatggaaaat ctcttttaat catcgaaaat ttatgttatt
      121 atgataacat aaagatgtaa atatcgagac gtctttttct tagttagatc attgacaagt
      181 gaacaaataa gatttgtcga tgatataatt aacttattaa aaatattaaa aaggaggttt
      241 ttatatgttc gattctatta aaaatttggt tagcggaatg acgcctgata tgaaaacttt
      301 agtacaagtt gttttgtatt tcttggcatt cgttttttcg ttgaaaccag gattcactgc
      361 aatgaaattt tttggtgatg caaaatgggg agatggatta aaatatgttg gggcgattat
      421 tgcaatcttt attattcctg taattacaac cgttatgctt tacacaatgg gtactaaaac
      481 aggtaatgat ttgaaccaaa aagctaatat catatcagca cttattccag ttgttggtat
      541 ctatctagtt tcaaaatttc caaaaaatga acagttaggt taaaagtgaa ttgaacaaat
      601 ttggataata tgccgatttg ttcaatttac tttttaccaa aaaactacgc tccaagcgta
      661 gtttagaagt gagggaagaa agtaaatgta agatggtttt tgatagagaa aaaatgttag
      721 ctcacgccaa cgaagtatta atgagcagtc tgaaaggtac cgaactggca aagataatga
      781 atatgaatgt taaccagttc tatgattatc gaaatgggag taaaaagatt gaaaaagcca
      841 gacttgaaac gcttattaaa ttcgaaaaag catatgtata tatgctagac aaacagaaaa
      901 gaactattga ctgaaaaaag gagttttaca ataatggcaa aattcaaaga tattaccgaa
      961 tctattaatt atgtggcaga aacagttcct gtagttgcac tctctttgaa gccacaatta
     1021 ctcgaagtgt acaaaaaaga agaaaaagaa gcgaaggcaa aaaaagctaa agcaaaggta
     1081 tggaaagaat tcgatgcgtt gctgaccaaa gatttaccaa agaaaaatta tatggtaatt
     1141 gaagatgtac aaaagctcaa cagtgaggga gttatagatg aggatgatgc tattctcgat
     1201 tctgaaaagt atgtcgttct attgaagtca agtttgaatt atgtagctga tctaccagaa
     1261 aaatttaaag agaagctatt agcaggtgtc cgttttatgg atagtagtgg tcaattatta
     1321 gttattccgg atgatcctaa tgtgtttgaa tcagaagttg aagaagctac agacttctat
     1381 agcgcagtag agcaattaga agtagctact aatggattag aacgaattgc tgagaccttc
     1441 aactcttctg aagaagttga atttgaagcc gtagcagaag atgtaccaga tgaaaaagaa
     1501 gctctctttg tggatgataa caaaccaacc gaagcagaaa accaagaagt aagcatagaa
     1561 gaaacacaaa tacaaacaga aacctaccaa caaccaaaaa ttgatgaaaa acccgaatcc
     1621 caaagtgaca aagaaaatta tgtttatgaa gctgaaaaaa tcacatctga aagtgatgta
     1681 ccgaaaaact tagatgctga ttttttagaa aaaaattttg aacagatcat tgctgacaaa
     1741 tttcaacctg tagttttaga tgagattaat gaccctgtcg atttaaacga ggagatgcta
     1801 tccatacctg agttgaaacc attgctgttt ttagctaaag agagtatgaa cgctcgtatc
     1861 tctattgcta ataataaaat caacagtgaa ttaaataaga cgaaggagca attgatcctc
     1921 aaaggaaagc aaatgttagc cgatgagctt ttgcggatag aggatgcaac ctcattaaat
     1981 ggtcaaaatc agtttacaaa tacactatcg gttgctaaag agagctacga agcagaagca
     2041 tctaaaattg atgatttagt gatggaaaaa tccaatatca ttcgtaaaga ttatgatatg
     2101 gcgaaacagg cttatttgga tgctgaacta aagaaactag agcaacggta tgatgctgaa
     2161 catttaccag aagttaaaca acaagaagat gaatatagaa aagcaactga agaaagtctt
     2221 gatcaacagt accaagaagc aattgaatct attaaaaacg atgcattgaa acaaagccaa
     2281 gaagcaaaag aagttgtggt ggaacgaatt atcaattcat tgaaaccaga aattgaaaaa
     2341 tcattaacag acgtgcaaga aaaaacttta gagattgtcg aaaaaacaaa gtcagaaaat
     2401 gaagctgagt tccatgatac aaaagagaaa acaacagatg tggttgtcaa atattttgcg
     2461 aacaaagaag aagttgaaaa gcaggtagct aacaacacga aagaattaga aacaaaactt
     2521 gtagagcaac aaaaaactat cgaagaatat aaagatcgaa tgaaagaggt caaagattta
     2581 gcagagggtt ataaattaaa tagcgaaaag ttggcagaac aaagtgaagc tttaagagaa
     2641 gacttatctc gttcccgaag tgatctagct caaatggcag aaaaaacgaa atcattagca
     2701 gttgctacag aaaatcaatc aagttcaaaa cgtgatttta caagtaaaaa gatggctact
     2761 atcattgctg tcgctgcaat ggcttgcgga acgatcttgg gatcgtccta ctttattagt
     2821 cataataatc aacaagcaca ggctcaaact caaacatcta tttcgagagt ggaaaattcg
     2881 attgggacaa atacgaactc tgcttcaaca aatagtagct atgaagcaac accaactaca
     2941 gagttcaaag ttgggaaaga tgttgtcatc agtattgatg ggaaaacaac taaggctgaa
     3001 gtgatttcta ttgatggaga aaaagcagtt gtgaaaactc cagacggaaa tcagtacaaa
     3061 attcaagctc acttatctga taataactaa ggagggctat tatgcgagaa gaaaaaatag
     3121 tttatccagt aattcaaaac aatcaagaaa acatgacctc ttctcagcac ttacaggaag
     3181 cacagctcgt gtatcaggta aatgaactac cacaaacgaa cgagacaatg agcaagtcaa
     3241 tatctttaat tggcttgctt attgttgcaa tagtgataac tgtaggtgtt taccgtaagt
     3301 tgaacgtgaa aggaagtatt tctgatggca gataactcaa actatggatt taaacataac
     3361 ttacatcaaa aaccttctga aacaattgtc agagttgaat ggcttgaaaa gcatgtcaat
     3421 agcacctatt ctgttacagc aaaaagatta attgtatcag ctatagttgt tgcagtcgtt
     3481 gttcttggat caattgttta cttagtttct gatacgatta aactgaaatt tatttttgtt
     3541 ccaacgttat taatgataat tggatttctt attctaatta gccagtatat gtttattgat
     3601 ccatctagtg gttcattgat ttctggttct attagactgt tcaaatcagt agttaattca
     3661 gcaagagtat ctatgggtaa aaagaaacca tttcgaggca cagagatttt aaaagtgaaa
     3721 caagatggaa caatcgtata tcgagatggg agtgtagcat taatgttcaa tctagatggg
     3781 gctacatcac taaccgcttt tccagaagaa gttatggctc aggaatcgaa atcaatacaa
     3841 tatcaaaata ccagagatcg ttccttagct gaaatcaaaa tcacttcatc tcaacctcag
     3901 aatacggaac gacaaactcg aaatatgaaa atacaaaaac gaaatgcaag gagtaacaaa
     3961 gcagtctttg acataataga tcagcaagaa cgatttgtat cagaatatat tgatggtgtg
     4021 aaaacaacag taatccaata tcttatcttg aaatctcaat ctaagatggt attggatgaa
     4081 ggtgtggaac gtttagaaaa ctatacgagt agaggacttt attatagtgt cactcagttg
     4141 tcagctaaac aaacaaaaga aatattatct gatatttatg cactgaaata gaggtgagag
     4201 attaaatgga aattatgggt agatcacaac tattaaattt caaaaaaaag agaaaaacta
     4261 aaaaagaaca aaaaactaga aattcaaagg tagtgaataa tacctttgtt agtgatttaa
     4321 catctttagt tggttatcaa tttacaccat cttacattag gacaggtaag cggtggggaa
     4381 caattgtgaa attagtgaat acttatggaa tgaatagaaa ttatggattt ggttggatgg
     4441 ttaaagctat tcctgaagtg agactagaag gggtaaaagc ctatctaatt gaagcagata
     4501 agccagtgtc acaaaaagat caacaagaaa taatgcacga gaatgttggg ggatcaacga
     4561 atagttatga aaataattta gaaggaaaaa attcagatgc tgaaattagc aaaactgtta
     4621 tgcggattat ggatttgaat acagcatctt tactagacag catggcaaat aaaattattg
     4681 attggaagct agaaatccta ttagtatccg attctcctaa taaaatcagc gaacagctaa
     4741 ttaaactcca acggttgtat gatgaaaaaa tgtatggatt aaagcttatg tcatatggag
     4801 gagatcaacg tgatctgttt gatagacttt taagtgaacc aatcgggaat gtctatgagt
     4861 atacgtctat gagttcggat tttgcaggaa atgatcatgc aattcgtaaa ggtttagatg
     4921 atgcgaatgg atgggctatc ggcactatgt ctactagcta cgcaagtggt gtagctttta
     4981 tggcactgga tgattctttt acaagtaaag ttttagtggc atcacacaaa aacgctacaa
     5041 ttaaaggcta tgatgaaaaa atctcagcat catcactttg ggggcaattg attgctaata
     5101 atgcaatggc aaatcataaa cgaacttttc atattgtctt aaataaattt gattactttg
     5161 gtgatccaac tggtagaaca tttgttgcca aaccttcgtt aaacagtgta atagaaaagg
     5221 ttaatttggc tgaagaaggt cttaactctt tggagctttt tggtaatcaa aaggacattg
     5281 gtgatttata tagacggaat ctcgataaac taactcagat tttttatttg ttagcaggca
     5341 gaacattaga ttctgcaact agagttttat tgaaaaaaga attaggtaac ttttatataa
     5401 gtcagaaact ttgggatatt aattatgaag aacgttcaaa acttttggtg aacagcagtg
     5461 atacaatgcc agttcttggg gattttatta gctatttgac taatgctgaa acaaaagcca
     5521 aaaaatctgg tactgaaaaa gaagtcgatc gtattattgt attaaaagac acattgagaa
     5581 cagcattaga cagcttctct aggatgttta atcaaagaac gacactaaaa gaaatcggta
     5641 aaagtgacaa attacagttt tactataact taggtgatct aagagcagaa atcaatgtta
     5701 tggaagctca atttttaaat acctttgatt tcatcacatc acaagcaaaa aaagatgata
     5761 ttgttatgat tcacggcttc gataagttat ctattgaaac cgttgggcta attaaaaaaa
     5821 gattaacaat gcttgaagaa gatggaattc gtcttgcctt tctatttgat acaatcggtt
     5881 caggagaaga caatgaatac aaagaagata ttgaaacagc tgatatattc aatacagatg
     5941 gaaaaattta ccaaagtttt gaaagtgatt ttaattacac tatcattgga aagatgacca
     6001 gtggagagtt aatgaagtat gaaaaaaaag ttgctcctgc tagaaaagga gaagatgatc
     6061 aaaaactttc tgttgatttg agacaatatt tgacaacagg aagaagtaat aaatttcaga
     6121 tcaggagacc agtggatctg actaataatt ttgttattgg agagtttatt ctttaagttt
     6181 tatctttgat tttgagtgta taaggaaagg ataagaaaaa acatgtatat ttgagactaa
     6241 aaacagtctt agagaacatg gtacgttttg tgaaaagaga actatagaaa tttcataaag
     6301 cgaaacctta ctgagtgaaa taattatagc ggaggttaaa taatgaaacc aaacataaaa
     6361 attttagatc gaatatttct aggtagagac acagaagtaa tcctaattca acatgaagaa
     6421 ggatttgaag ttagtatagg tattcagaaa cttcaaaaac ctcattattg taatcagttg
     6481 tataaaaact ttactgatga agaaaaagcg agagtttttt ttaatgaagt caaaggtatg
     6541 agagaacagt atgaggtggt tgaagcatga ataagttacc agaaaagaac cgtgtattta
     6601 gattgaaagg tgatctcagc caatttaaaa tagatgagaa tggacaattt tttgtttgga
     6661 tgcgacatga gcattgttgg tgtaatcctt ttgagttaaa aaaagaaaat gaaatcagaa
     6721 ctaaaattgc aaacggtgag tgggattacc tcttcaggtg ggaagatgaa tcttttatag
     6781 caaatgatat ggatcatgca tttgtctcat ttctggataa tacatggggt acttggtacg
     6841 attgttatct agaaaaattg ggctttcaaa atattgatat tgatgctatt gaatgtctta
     6901 aagaagtcga tcaaaagtat gaagaagaac cctattatct cttgcaacaa aaattagcca
     6961 ataattcagt atttcaggta gaaatttggg aaaatgaaga tggaacttat gaggggctag
     7021 cgcaagaaat acccttctca gataggaaga gttcgatttg aaaagagtat atcagcaatt
     7081 tttatgaagt gaaagcaagc ttacaatata gtaatggaaa attgtggtta gctggaaggt
     7141 atattctagc taaccaaatc ttacgttttc tgggagagtg gcgttttaaa acaatttcct
     7201 aggaaaaata gttaacataa aaggaatgaa ttgttatgaa acaatattta aatgaatgga
     7261 aagcgataga ggggtcatta gtaacgcaaa gaataaagga attacctgat tgcttagaga
     7321 aggatcattt gtttcaaata cgagaaatgt tgaaaaaaga acagtttgat ccagaccaat
     7381 ttttagtagt cgagtatcca actattggag tgtactgttg taaccatatc aatgatggaa
     7441 aatattttat tattcaggag tatggaggac agttaactcc tttctatact acatgggaaa
     7501 tgagtgaaga aggaatcaat aattttccat gtgaaagcat cgaagattca atttcacaca
     7561 ccgaatgtta attgattata actataaaaa aggattgaac attatgttca atcctttttt
     7621 caatgaaatg ttttccgcca tttcattgtt tggtgcgatt tctattttat tatatcaaaa
     7681 taaagagaga ttctaaagaa agaagaggga aatatataaa tcaagcgaca gtatatttat
     7741 tattaaatgc tattgtgaga atataactaa ttagtttaat ttgaaagtaa atatacgcta
     7801 taaaagtgca tcttgtgtta taagaaatta ttttattttg tgaacataat ttaacaattc
     7861 tttctcggaa ttgatttctt ttcttaaata gtcttcagac ttattaataa tattatctat
     7921 tatgtctaga tacatatcac ttttcgattc agctaattca tacaggtttt ttaattcttt
     7981 atcctttaat tgaaaaccct gatccatagt cactgcattt ggatatagaa gcccactata
     8041 atgtttacgt aaattcactt ggaatttctg attataaaag tctaacagat tagaaataaa
     8101 aggcggaaaa ttactgtttt tgtaaaattg attatttcca tttaggttgt atagtaattt
     8161 gttaaattgc tcctgattta atttattgta aatccatggt aatacttcca tatccttatc
     8221 tgctaacctg aatgaactag catcggtaag atgttcgatc ataaaatcta tgaacatctc
     8281 tttttcacca aaatataaat attgattata tattgtttct aatgttgaag atggtagcga
     8341 agaaactctc attttttgct gaagtatagg atcaagtaga ctatttaagt gattttgtaa
     8401 ttcttcattg ttgcttccta gaactgcaag tgaacgtaaa cggtagtaat attctatttc
     8461 tgttagtact tgttgtttcg gtttaaaatc atttattgat atttgtaaat tttgaattgt
     8521 ctcgtctgaa aaatcatgtt gtaattctgg atgggtagct ataaaagtaa gtaaaaagac
     8581 cccttttgta tatgtgatct gttgattttc accaagtgat ttattaattt cctctcttga
     8641 ttcagctgtg aacctatact ttgaaatttc ttcttttaga atttttaaat agaattcttt
     8701 attatcaaaa attagggcat taagtagtct gaaagtactt tccctatctc tatctgcttc
     8761 ctcatttcca taaggtaatt gaaaaacaaa cttaaaggct gtcttacata ggttttcttt
     8821 tgctttttcg cttagtttat ttagatagct attttttagt gcagagtgaa attcttcagg
     8881 ggtaacagac atagcatgta tgcgacttgc atattgtata atttcgatcg ttactttttt
     8941 tcctaatatt ggatttttta ggaatataat ttcataggac ttccgtataa catcctgaca
     9001 agtttctttt gttatatctc ttaattccca agtattaccg ttgtaagtga ctacaggatg
     9061 agctgaataa tttctttgat ccttaatata ttttaaatcc tcatattcat agctatcaat
     9121 tagctcagtt gaactataag ctttttctag tagttccatt tcccattttg gagaatttgg
     9181 atcacttttt cttaatctag ctatatcttc taaaattttc ttagaagtaa tatcctcgta
     9241 aatatttgct aaatcagata atttatcaat taaatctaaa acaagcgcac tgtaattgac
     9301 agctattgca gaacgataat ttccctgata aaaactactt cgaacttctt ctaataatgg
     9361 aataattcta ggatttttaa ctttttttat ttcatcatct aatataaaca tataaatctc
     9421 ctttttttgt ttgtatgaca agtttaccat aaaaaaatat taaaaagcga gacaaattta
     9481 tgttattatg ttatcatgat agcataaata tatggagcga gatgatggaa aaatggattt
     9541 gaaacaatcg ttttacatct ggttatatcc tgataatcct atgtcaataa tggctgatat
     9601 ttttgtagtt agcatcttat tgtatttatt ttatttaatt ttctttcaca atttaatcgg
     9661 gtacatgatt cttgattgga aaggaatgtt cgatatagag agaaaaccat ttgctaacaa
     9721 tcaaacctat accgtcttcg aaagtggaga ttgggaagaa aatgaagttg atgtgatcca
     9781 tgaaattgga ttagctttat caggaataca agatgctgat gttttggtat caataacaac
     9841 ggatactgga tacaacgctt atgaggtatt ttatcttatg tattttatat ggcgtgatgg
     9901 tcaagaaatt atcaatggtc gtgctgaaaa aacaatcatt tctaatactg aaatttcttt
     9961 ctgtatgggt gaaggggaaa tgattgtata taaagatggg tcagcaaaca ttaaaacagc
    10021 ctttggtgat attgatacaa gtattaagga acttagggaa tatgtgcaga acccatataa
    10081 ttttcaatat ttagcaggaa gaataaattt agtttaggag taaaaatatg aaaaaaatta
    10141 ttaatcaacc aacaaatcac ttgataattg aaggtgttat tttcgtaaca atgtatttta
    10201 ctgtaggaaa tggcatctca aatacaatca ttcaaaaaaa tccacaatac ctattattta
    10261 gtctttatgc ttggattgtg caaatggttt gggtttccat attgatgctg ttgttttatt
    10321 ggatagtacg ggttattctt ttagtgttaa acaaacgaaa aaaggatagg tgaataaaat
    10381 gaaatcaact aaatggaaga ttgtattgtt atcaacagca gcattaataa ctcttacagc
    10441 atgtggtaca actaatagtg ctgataataa caaagacaca aagtctagta gtacggtggt
    10501 tactaaaaac agctctacat cggcatcaac aacaatttct tcaatgactg aaaataatgc
    10561 tgaaaagtat caaaaatacg aagacattaa taaaggtcaa aagccggaag aagtttatac
    10621 cgactttatt aatacagtaa aaaatgatga agattatggg aatgatccat tgcaaaataa
    10681 aaatctgact atcgttcttt acaaacctga ttgtccagat tgtcaaaaag cagaatcaaa
    10741 aatcatgcaa gaaataaaag aaggatttag ttatgctcca acattaatgc atgatattac
    10801 cataaataaa ccagcttacg agtatcacgc agttgctgta gatattactg atggtgtacc
    10861 tgagtggata caaaacattc ctggtctggc tgatgagaac aatcaatatc atacgccaac
    10921 agttgcagta gtaacaccaa agaaagttaa tggcacacta tactgggatg taatgggaat
    10981 gacaacaaat acaactgatg aaatgattga aagtacaact aaatattata tggatttggg
    11041 agcatataaa ttatatcaac agtcagaacc tgttatggac gatcaatcca aagttaaata
    11101 gttctttagg gcgtaagccc tattttttta aaattaagtg ataacaaata aatgatttct
    11161 gaaacaaacg ttcttactca actactcgaa acacacaagc taattaacta gattataaag
    11221 gagaatgcgt aatgaaagaa atcagtgcaa atgaagctca tattccgcaa aagaatcagg
    11281 ttggtatata taaggtgctt catggtgaag gaatttataa aatacagtta cttaccggtt
    11341 acaataacgg tgcaaatggg aagtggttaa aggcaaaaag tattcgtgaa cttaaaactg
    11401 aagtagaaaa aataagactt gggttaacaa gggaagaaat aagtagactg agtgaaaatt
    11461 cttcttttgt aatccaaaaa gaaaaggaga aacatctact tcatcgggaa ctaataattg
    11521 aaaaatacta cgatgttgat ttacctgatc agttaagaat tattgacgaa ttttcagaga
    11581 aaaagtggaa atctgaaata tacccaagct taattcaata caaggcatca ggaaagccca
    11641 tgtataacta cacaggcttt ttaaactaca cacaatataa atatgccttt cgcaaattaa
    11701 aaggcattaa acaagctgac attgccctgt atgagatgaa aaaaatggat agattgaaat
    11761 tgattcaaga gttagtgcaa aatacatgga atcaagcgat tgtcgaacta gaaaaagaaa
    11821 atgggaaaat tttaactaat gaatatctga tcgtaaatga aattgaagaa ttaattggtt
    11881 tattttcatg tgaaaatggt gaagtcttct tgaagtcatc accaatctat tcaattttgg
    11941 acagtccgta tcagtttata caggtaagga gatgagttac atgtctagct tgaaagatga
    12001 ctatatccaa gcaaaacaag agtatgaaaa aatacagatg ttaaaatctg agaaagtcga
    12061 attattttta acacaaaaat tagcagagga tcatattgct aacttagatg ctaaaacttt
    12121 agaggaatat gatcagcagt ttaatcagac acatcacaat atcattttgc ttgttgatca
    12181 ggcaaagaga agacttgaaa aagcggaata tgaactatta gatcaaggaa ttatatgctt
    12241 ggataaaggg attcgaagat attatagtgc agagttaaaa aagcaaaatg tagacagaca
    12301 tgcgttaaaa catgaaatct tagaattgat aatgcaacga atttgaaggg atagaaagta
    12361 aatgtcacaa aacattggtg ctgtaaaaca attaatagaa aaagatttag agacgacagt
    12421 tcagaggtta gccgaacgaa tccattttgc aaattatcaa accatctcta attggtcaag
    12481 caggaacaga aatccggaag aaattccaat gaaatttttt tatcgaatcg gaaaagaata
    12541 tggtgttgat ccgataaaaa ttttcgaaaa gaccatgatt tattactatc atggaacacc
    12601 taacgaagtg ttggaacatc ttttggaaga agcagaagaa aaatcatcaa tattagagat
    12661 atcacaaaat gacaaagcta ttcaagtgaa atgtgttgat ccaacaggaa agattattgt
    12721 tggatttgat attgaaagaa aatctaaaat agattttatt actcaattaa caaagataaa
    12781 taatttaaat attttaacac ttagaccaga tcatgaacag ttgtttatga gttttaaaaa
    12841 acaattgaaa gaaatacaaa aaagatataa caaggtgttt tctaatgcgt gaaaagctaa
    12901 aagaaatcag agtgttttgg ataattaaca aagaatttat tatcttgtat acacttggat
    12961 ttatcagcat gatcttgttt tggtggttat atttaaaaaa gtaatttaaa agtgtcaagg
    13021 agatttttat ccagagaagg atgtggaatt tgcatgtgga ataataaatt aaaaacaggc
    13081 ttgttattaa tagttatttc ctgcgctatg atggttggca tgataataca aagggaacaa
    13141 tcttattttg aagtttctgc taacaatgta atagaaagat gccattatgg tcaacatttt
    13201 tggactgggg aagtcagaga aaatatagat cgtgagtatg ttcaaagaat cgtttgggat
    13261 gcatattcta tcaaagatta tcctaaaagt ttaacgagta gattatttta cagcgaaaaa
    13321 gataatcaaa aactctcgaa tttgatgatg aaaaaagtaa ggaaacttgc acaaaactat
    13381 ttggaagaaa aagaaggtaa tagtccaatt gacaaaaatt gatgacactg caaaaaaata
    13441 tagagttcga attagctttt ttcggagcat tgttttttag gtgaactata tttcatcact
    13501 aaaatcagag ataactaact tgaaacatgg tcagatgtgt tacttgaagt ctctgaataa
    13561 tcaatgttat ctagtagaat taagcaacta aaaaagaaag aagtaagaaa atatggcata
    13621 cgaaaattta caattagtag tcgcagaagt tagtggggat atatacctta caaaagtaaa
    13681 agatggaatt atggacacga attataggag agttgctaca gatgatgtgc taagctcatc
    13741 gacagaatgg tttcgaaaaa atgaagaatc tgctgttcac tttgaaggaa ttgatggaaa
    13801 cattcatagt ttgttctata caaaagacaa agaaaaagca aaaaaaattc ttgatatttt
    13861 gaaaggaagg tagttaatta accaatgata taagagaatg tgagaccaag taaatacttg
    13921 gtctctataa ttttagccac tagtcaatta aatagctaaa caattttaat aataataaaa
    13981 tatgactgct tcataattaa gaagtgcaga atttttctaa gaacgatatt tccttgttga
    14041 aatgagtatt gctaacaatt tgatcacgaa cagcatcgtt acaaatgttg tatcaatatt
    14101 tgatagatta catattctat taaaaaagaa aagaaaatta ttgatattat acacattagt
    14161 gtgtataata aaagagaggg agtgattaga actttgagca attcactaat tgtttaataa
    14221 tagaacgatt cctaagaagt tcattgttca ttggcaaatt gctttatcag attgaaagtt
    14281 ggcgaataag gtttgtagaa taacaccttt tcaagcaaag cgatttttga aagctatgaa
    14341 atttgtttaa taaaaaaatt caccagataa tctgatagag actaacccaa ttactaacag
    14401 ttgtaattgg tgttagctta caaattaatt attgtgttta gtcaattaca aattgtgaaa
    14461 cggcactaat aattggaaag gaaaaatgaa atgtctaaag atttacgaga gcttaaattg
    14521 cgaaaaaagc aatataatca atatttttct agtttgttga ataaacatat tgaagaagga
    14581 cttgatcatg attttggaaa agatgaatat tgtttatatt gcgaacgtta caatgaaaaa
    14641 atgtctgaat atctacagct gaaaaaagag atacagaaat tagagaaaaa agtacatgct
    14701 tggaagtata aagattccag gtctggttat gttgaatctc aaaataagcc aagaaaaaag
    14761 aagcaagcgt atgttctaaa gatggtaaat ggggaagagt taattgtatt agcgtacagt
    14821 gaagaagagg ttcgagaact cgttgaaaca cctgtcagga aaaaggttga aacaataaat
    14881 gagatagacg acagccaata caatagcatc tatattgaaa caagtcatgg tggatataag
    14941 aaattagcaa ctttccttag aagttctaaa gttgggataa tttctaaaaa agacataagt
    15001 gctgttaccg atagtttttg tactttatct gttcctaaag cttatttcca atacgatagt
    15061 aaaagatttg aacgcttaga gaaattctgt agaccgtata ctgtattgaa aactaaaaaa
    15121 tttaataaaa ctaaaagagt ttcgatacag ttaccgactg gtcaaatctc attaggtgaa
    15181 aaagactatc tgataaaagt aggaaaagag ttaattataa aagtaccgga acacttattt
    15241 aaagaaaatt ttgagatacg tgaaaccttg gtatggaaag aagaaaaata gtttggaaga
    15301 catagaatgg tataagggga aattgaaatg gcagtaaata aaatattgca ggtattaatc
    15361 atagacgaat ttggcggagg agaatggttt gatgtgcagt ccatagactt tgaagaagaa
    15421 gtcgtagtta tagaaaatat ttacggcgaa gtatactcat attcgcttga aggtgagaat
    15481 gttgaggatc tacggttgat tggtgagtag aaaaaagcag aaatatcaaa atggagataa
    15541 aaattaatag catgtaaaga tagtcatgct tacatgcttt atccaaaatg gtatgcagac
    15601 aaaaatatag ctgaaataaa cgattatcta actagtataa aggaacgcaa tgcaattaat
    15661 taacaataga cgaaaaatga aattaaagga gataggaaaa tggtagaagt tgaatttgga
    15721 tcaaaagaat ggtttgaaga gttaaactca atgattaagt tcccagaaaa gtttactttt
    15781 tactacaata aaagagacaa tatcattcaa gtagctttac ttgaaactaa gggaagatta
    15841 gaaaatactc gatggagtat gataaaacca tcggcactta gatttcatcc taaagacttg
    15901 acagcggttg tagcggtaaa agaggttcaa gactcacaag aatattatga agtagtaaaa
    15961 aatctgaagg atatttggtg taaataattc taaattgacc agttatttgg tcaaaaatag
    16021 tatgtgatta tttcatccaa tgtggcatca taattggtat ggaaggggat aaattatttg
    16081 aaagaaatag aaggtattaa gaaaagaaat caggaaagat tggaactgtt aaatgaaaat
    16141 agaaatagaa atagaatgtt tagttattat gaatctatga ggtgtttaaa taaatctaga
    16201 agtgaaatta tagaatcatc aaagtataaa caattgatgg aagagagagt ttctctcatc
    16261 caagaaatag aaacagagtc tatttttcca gatcattctc ctaaagtccg attttataat
    16321 taaacattaa caaatgaaac gatgctaaat aggcatcgtt ttttatctat ttataaaatt
    16381 aatttattta caaaatgtgt atcggtatgt atggtgaatt tgtagataag ttgagaaagg
    16441 gtgatcttaa atgaaattag aaggtagctt acaaaataga atgatggaag gcagtacaac
    16501 aaacaaggag attgtagttg gaatgggagc aaccgagctt ctatataccg acagaaatcc
    16561 ttatacagta attgaagtaa aaagcaaaaa cagaattttg gttcaagcgg atggagcaat
    16621 caataaagct acttttccag atcaagagtg ggaatatagc agaaatcctg aaggtcaaat
    16681 attggagtta gttaagacaa aaaatggatg gaaagtgctt aaagaagata ttcattttta
    16741 tataggtgat agagaaaaat actacgatcc tagtttttaa acaacctgaa gatgagcaag
    16801 gttttatcct cgctcagaaa ggagtatgga ggatgttcag tcaagaagaa atcatcagca
    16861 aatcgcaacc ggataaaaat ggaactataa tcatgaagaa aaaaatccct tgctatcgtt
    16921 gtagcggatc aggtcagtac aagtttatgt ctatggggaa aattacctat ggcacatgtt
    16981 ttaagtgcga tggtgatgga tttcttgttg taactgaaaa atcctttacg ccagaatata
    17041 gaactaagct aaatgtttta aataaaaaaa gagctgatcg aaaaataagg atcgcaagag
    17101 agaaagaatc tcagaaaaat tttgatcgtt atggctttga aaagggttat atttacgtgg
    17161 ttatagagaa aaatagctat acgatcaaag atgaactgca tcatcaagga gctacttatg
    17221 atcctttatt aggttggttt ttttaaggaa aaacaagaac aatacaaaac tcaaaaagtt
    17281 tttgtagaag aattttctgt aatcaatgat gaaggaatta ttgagttgaa gctagaagaa
    17341 tacagcaaaa gacaacagga gattttaagt gcgaagcgta gagaagaggg tgagtttatt
    17401 ggtacaatcg ctcaacgatt ggaattggat ttgaaactta ccaataccgt caccatagat
    17461 acacaatggg gcaatgttta cctgcattta tttgaagatc aaaaccataa ttcattgatt
    17521 tggaagacaa ataaatttct ttattatgat gaatgggatg ttggagaaat aagaaaagtt
    17581 aaggcgacaa taaaagcgca tgactactac agagatacca agcagacttg gattaaaaat
    17641 gttaaaattc aaaaaaatga gtaagaaggg ataaaagtat gaaaagagaa gaatacaaac
    17701 aaagattaaa cgaactgctt gaagaggacg aaacattaac tcatggttcg ccggatgaaa
    17761 ttctatacat gattgataat atggtaattt ttggtggata tgaacttggt aataggtcag
    17821 ttgatcataa tattttagaa tttgatgatg tatcatggga agaaattctt gattggggaa
    17881 ttttggcagt acctgaaact aaaacctata ttagtgacac tatggttcct ttttttgagg
    17941 aattggatta taaaagactt cccaaaaatg aaaatcatat actgggagga aactaacaat
    18001 gttaagtgac ttacttattt tactgatcta tctaggtgga actgaaatta ttgcgtattt
    18061 tttgcttgta attggtgaat atggtatttc cagtgcaatc actcaaaggt ttagattacc
    18121 agtaagtgaa ctaaaaaaat gatgagatta tcagcatatc ttttaatgtt tttgaatgtg
    18181 gtcatgttaa tctttgtagt aatcggtgtg actttcaatt taacggaata gagggaaatc
    18241 tataatggaa aaaagagaaa agaatgaaca tattttttga gtcaatattg ttgatggttt
    18301 ttgcgatcag cgcttaccag aaatctaaaa aagagtaatg actgtttagt catcactctt
    18361 tttttgtagg gaaattgaaa tatgtggagc ttgtattggt ttagttagga ttgcttggct
    18421 ataaggagct agttctttat caaaaccatc aaagtaggtt ttcagtagcg atttaactga
    18481 tcgctcatct tgtttgattt ggataatttc agcatccaaa tcggttgtga ggtcaggagc
    18541 ttttgaagcg attacctgat tttttttatc ttccaacttt cttaggctgt ttaacaagtt
    18601 gtaaaaataa tcattttgac tgttcattcc aacgtgagat gcaattttaa cagaagtttc
    18661 atccgtattg gcatcaagta tttcatcaaa agaactcatt ggaatatcaa gtagtaacga
    18721 ggatgtttcc tttacagcat ttttagtttt tccatctttt ttgctcttaa cttgagatat
    18781 ttttggatct agtttaggta aatcacttgt tttatcagtc atttattagc ttttcctttc
    18841 tattaattta tgttattata ttatcataat aacataaaaa agtacacttt ttgggggcgt
    18901 agttattgaa aaaatctaag agaaagattg ccacgttgct tacgttaatg gtgcagttta
    18961 cagggattgc aatgagtgta atcacaataa atccgcaagt gttacatgct gatgggatag
    19021 ctacttatga tgcacaattt aactgtacta ctgaaggtag tgggtcagga acagatagcg
    19081 atggaaacga tactggaagt ggcgctgtaa caggtgatgt agccaaaaat attgaagata
    19141 tttacaaata catgaatgga gaatatggct tatcaggtgc tagtattgca ggtattttag
    19201 gtaactggaa acaagaatca ggtattgatc ctcgaacagt agaagggtat tacggcgatg
    19261 ggcagagaga agttgacttc atcaaacaat ttactgatgg tctaatgggt attggctatg
    19321 gtcaatggac agccgggcga catacagcat tagttgattg gtctaagaaa aataatggcg
    19381 gttcttggtg gactacagaa gcccaattgg actttatgtt caaaggtgat gggggattcg
    19441 taggaatatt aaaagattat gctttaaatg cttctgatga tgtagtagaa aatgcagtaa
    19501 cttttcataa aaactgggaa atttcagctg attccgaatc agctgtaata gctgtacgtg
    19561 gtgaaaatgc aaaagaaatc tggaagtaca tgaaaagcaa gggtatggac ggtgcaaaag
    19621 atgaaagtaa gatcaataaa atatccggag gttcaagtac cggagatgga tcgaataatt
    19681 catcaagttc agcatcagga acagtagaag atgcctgtgc aactacggat gatgctaatt
    19741 ctggttctgg atcaattggg aaagaaggaa aaattaacgg taaaaaaggt caagtaatag
    19801 caaatatgac ttatgaagaa gcattaaaaa aatatgggaa agcaatcacg ctaccaaaat
    19861 ttaaagaacc agactggtca acatcacctt ttcaaggtga attacaagga caatgtactg
    19921 aattaacatg ggcttatatg agtcaattat atgatggaaa tcaaccaact ttgggaaatg
    19981 gcggttacgt ttgggaaagc tacaaatcgg caggagcaaa ggtgacgaac aaaccaattg
    20041 taggttatgc gtttagtgct ttagatggat ggttatgggc tgttggatca gcaggacata
    20101 caggtgtagt tatagcggtg tttgacgatg gatcattttt gtgcgctaat ttcaatgttc
    20161 cacctaccca agcaccaaca agaggtgttt cagtaacttt gattgatggt gtggatgggt
    20221 cagataatgt gcatttcttt agcggtgtag gcaaagctaa atttgaaatt gaaaaataaa
    20281 aatttgaatg gagggcgaca gagatgaata gtaaaacgaa agatgcaatt atcatttctt
    20341 tgctttctgg tatgctatta gcaatgcttg ttggaattct atatttcaat aagcagaatc
    20401 aagacataga agctgaaaag cacagtgtgc aaattgaaaa tgaattgacc ttctttaaag
    20461 aggttaaacc aaatgaagtg gatggattgc aaagagattt agaaaatgat attcaagaga
    20521 tgtttaaagg atcagaccga agcaaatttg cgaatcagtc aggtgaagaa tttttgcgaa
    20581 agttatttat tgtgacaggt aagccaccag ttactaacga gagtagcgag caagaaaaaa
    20641 tagaatacta taaacctttt acttttaagc taacaaactt tggaattaag acatctagta
    20701 gtgggttgta tgaagcattg actgatttcg aagttcaata caacggaaaa aaagtgtatg
    20761 ataccacatt aaccatcaca ctaaataatg ataagaaagt gactgggggt gctttttatg
    20821 caaaatcatg aaattactac tgctaaaaag gcgatcggaa tactttccat aattttgatc
    20881 ctttgttgtg tggtactatt tcaaaagaaa aatgcttatg ataataatgt caaagaattg
    20941 gaagacctta aaagtaaaca aatcgaagta gtcaagaata accagaaagt gaaagctatg
    21001 caagaagccc aatacaaaga attgggaaat gctgaaatca aggaacaaag taggaaattc
    21061 tttaatttat tttacaactg gaattcatgg gaaaaatata cgaagaatat gaaggaaata
    21121 caaaaagaat ttcctcagat agtaaagtct agtcctgttg atatctctgg cacaatggta
    21181 ggtactggta atagtccaat atctagctat actgctgatt actatacaac aaatcaaaaa
    21241 ggtaaaatgg ttgaattgat tacacagaca agaaatactc caacaacagc aacaacaaca
    21301 atttggcgtg gagtaacaag cataggagaa aatcaattgt tttacttgga ggatttagat
    21361 agctatgtaa gagcagagta aaataaaaaa agagctaacg gtaccaacgt tagctctttg
    21421 tgctgacgat gataacaaaa cctaccgaaa aaggattgtt tcatcacaaa cacaaacaga
    21481 aaagaattga taaaaaaccc gaacctcaga atttttatta atcaattcat tttgattata
    21541 ataaattgag ctgaacatta caatacgtat atgttgtaaa acgtaaaaaa agtcaaaagt
    21601 aaatactttc ttaaaaagtc aaataaaact attaaagaca atatatatgg tttatttgac
    21661 ataagaaaat ggtctagatg ataattagaa tatagaattg tagacttaaa taaatcataa
    21721 aaaaccttct atgataagtt atagctctta attttaagca gttttagata taagaaagta
    21781 aagtggggga gaataataat ggcagatcgt aaattagaaa agttattaga agaaacgtgg
    21841 gatccaaaag aatttagtga attttttatg gagaattttg aaactgactt agcggtgatt
    21901 gttaaagatg ctcttagaga gcaagggtat cctgaaactg caaattatat aaacattaat
    21961 tttactttgt atactgaaaa tagaggaact tgggattttt gggctaactt agcaaataag
    22021 gagttaagtg ataaatcaga tacaggaatt cgtaactttt ttgaatcaaa cagagatgat
    22081 tatatgtacg ctaacaatca aaataaattg aactttagag tagagtttga tgaaacacct
    22141 gaagaattta tagaaagaca accgcctaaa gaaaatgtag caaaggtatt ggaagatcgt
    22201 tggaattcag atgaaatagt ttcaacaatt agcgaacaag acggacaata tgaaccgcta
    22261 gtagaagcgg ttagagaaga gctaagactg aataagtttc ctgatgttca aaatattgat
    22321 gtttctcaaa ttgaaataaa cgtcaatatc accaatagac ttgattatga tagttgggca
    22381 gatatagcat tagaaaaata tatttactca acgttaaaag agtttatcga aaatagaatg
    22441 aatatcatgt atctacagca tccacagtat ttaaactttg gtgttgaaat agctacacct
    22501 cttgaagaat ggaaaatgga gcaagggctt gactagcctt tgctccattt ttttatttta
    22561 caaatgaata cagttcttcg tctttaccaa gaatgattgt ttgcccaact ttgagattat
    22621 caggtgatcc ttcagcggtt tgtaaggaga acgttccatt gaacttatcg gataccgtta
    22681 taggtagctt taattctgat ccttcctcat ctttgagaat tgctgttttc cgttcatcaa
    22741 aataaacagt ctttattaga taatttttag tagtggtttc atcagatttg tctttgtttt
    22801 tagtagtact aatttcttta gaattagatg tattttctat gactatttcg tttttggtat
    22861 ttgcgcttgt atttatttga ttgaaaattt taatcgacaa gatcccacta agaatagcca
    22921 acatagaaac aagtgtaatt aatactgata atttcctttt tttgttcatt tcttcttaac
    22981 ttcctttttt gttttgatta gttagatatt ccttatctaa taagcctttt taggctatta
    23041 aatgaagata aaccccaaaa aattattgca attatgatag tgcaatggta ctattattat
    23101 aaaataaatt ttgcgaaagg ggtaggtcta aatcgcaaat aaaaagaaat tgaacctaga
    23161 caaatacaac ttgtataccg caagggaagc aagtctaatt ttgggtaaag gtagaaatta
    23221 tgttacaaac ataatcaact attatccaaa tcgtttgcct aaaggttcgt tcaagaaagt
    23281 gggtgacaaa acctatatta taacggaaga aggaatagaa gcattgcgat cacaaacaga
    23341 aaataaaaac aaaaagaagg aatgaatatg aagaaaaatt cgcttgctat ggttttattt
    23401 gcatcagctg tacttgcggg gttatcagta ccagtaacct cacatgctga tgaatggact
    23461 gcaaacacac cggatcaaat ccaaattaca acaggacaat caagttatgc gttcaaaaaa
    23521 ggagatacta tttggtcaac tgtagagaag cttaaaagct taggttacaa gactactgtt
    23581 gctgagtttg ccgaattgaa caatgttgat ttggcaaaag gagaacaacg gactatcccg
    23641 attggacgaa ctttcaacat caataaagat acattaacag ttaaggaagc agatggcgaa
    23701 gtagttagtc aaactgttca aaaagataaa cctgatcctg ttgaaaatac ggagcaagag
    23761 aacaaaaaat ccgagactac aaaagaatcg gaaccaacag caaacacttc tgataagaca
    23821 gaaacaagtg cttcaagtgt tgatggagca agttcatcta atacaaataa ttctacaagt
    23881 gagaaagaag catcacaggg aacaagtgaa agtagcacta cggaagctga tactggaaca
    23941 tcaacaaata actcacaatc aacatcaaat gatgcaaatg aatcaaccag tacatcttct
    24001 agtgatcaat cacaaacagg ttcaagtgat gaaactgaca gttctacttc accaagtaaa
    24061 cccgaacaac caactaatgt tgtgctaggc gttccattta ttagtcaagg taacacaatg
    24121 ctttgcgaag gaacatcttt gctagaagcg ttacattata aaggtgttgc aacaaatcaa
    24181 gatttaatga cttttgttaa atcaatgcca ctatctcctg ataataatcc aaataatggt
    24241 ttttctggtg aatggagaca caatgtggat ggaacttatc aaggtatgaa tccggctcct
    24301 gtagtttcat ggggtgttgc taatggcgga aatgttgtag atatttctgg ttcatcggta
    24361 caaggattaa aagatgagat tgtaaatgga aatcctgtag tagcgtgggt aacttatgcc
    24421 tttgaacctg ctcaacataa atcaatgttt tggggcgatg ctatttggaa tcgacatgta
    24481 gtagccgttg atggatttaa agacggtttt taccatgttg tcgatcctgt gtttggggca
    24541 agttggatta atgcaggatc atttgaaaca gcatataacg tatctcaatt agcggtttcg
    24601 gtacgttaat aaaagagatc gtttatatac taagtagtta agtatagtct ttatttgtcc
    24661 ttgcttaact acttagaaaa cttgttgctc atggttggta gaactaaaag gggtgatgct
    24721 atgaatacta gagcgttgaa acagcttgct ttcaacgaaa aaagtgatta ataggtgtgg
    24781 tgattttaac ttaggaatgg gcgcgaaggt cattaccaca ccgacatatc gtggctcatt
    24841 ttcttcgcct aactggtata aagaaggagt aacgagagat gaagcatgaa aaaacgataa
    24901 ttttaaaatc aaaaataatt tattgttttg cgactttagt aatggtgtta tctacgctta
    24961 taatgccttt ttcagcaata agagctgaag cagatgtcac aaaaccgaca agtgcaaaat
    25021 ttacagaaac tggcgtaaaa acagacggtt atattagggt taatatgccg aatcatccaa
    25081 atgaatggat gatttcaagt caatttaaag attctcatgg aaatattgga tattgtatgg
    25141 attcagaatt gccatcccca acaggaagtg gagccggaag cttgaagtac aagggagctg
    25201 gcagtgatga gttctatcgt atgtttaaag gtggttttcc atcaaaaaca gcaaaggaac
    25261 tgggtgcagg aaatgataca gaagcatggt atgcaacaca attagtttca tgggttttag
    25321 ccggaaactt taaagtttca cagattgtat ggtcacatcc aaaccatacc gccgcagaaa
    25381 cagctcgtgt aaaaaaagca tttgaaaaaa tttatgacta tgctaagaat ggaaaagata
    25441 cgcccaacac tgagttttct atcacagctt ctaagacagc tgacgaagga aaatatcaca
    25501 ctttcacata taaaacagca tcaaataaga ctggtaatgc gaagctcact tttacatcag
    25561 caaaaccagc tggtatgaag atttatgatg cagatggtaa agaaattact aacaacactg
    25621 tgaaattaaa ttcaagtttt actattaaag tacctgtgac tacaccaagt ggtactttgt
    25681 catttaaagg tactgctaat gttagcacaa ccaatccttt tacttttgat ggacgaggtg
    25741 tgtatcaaga tgccgtagtt atgatcacta cttcagaaac aaaggactct aaatctttga
    25801 gtgctaaatg gacaagagct gttggtcagg taacagttaa gaaagttgat caagatggaa
    25861 atgctttaaa aggagcggag tttactctaa ctgacaataa caatgctaaa tctgttaaga
    25921 caaccggaga tgatgggtta gctacgtttg atttgcaaca aggcttaaca tacagcttag
    25981 aagaaacaaa agttcctgaa ggttatcaag gttcatttaa aaaggatggt attactgtta
    26041 ataccgatgg acaagaattt agttacactg ctacgaacac aattaaaaaa ggtaaagtaa
    26101 aagttaaaaa ggttgaccaa gatggaaaac cattagctaa tgctgaattt accttgacgg
    26161 atgattcatc ccaaacacaa gcagaaaaaa cagatgaaaa cggacttgca agcttcgata
    26221 ttgtagcaaa caagacctac agccttgaag agaccggagt accaactggt tatacaggcg
    26281 tattcaagca agaaggaatt accgttaaag atgatggaca aacttttgaa tatacagcta
    26341 caaacacttt gaaaaaaggt ttagtaaaag ttcataaagt cgatcaagat ggaaaacctt
    26401 tggcaaatgc agagttcact ttaaccgatg aaaaaggtgc aacacaagta gaaaaaacag
    26461 atgaaaatgg acttgcaagc ttcaatattg tagcaaacaa gacgtatagc ctagaagaaa
    26521 ctggagtacc agtaggttac acaggtgcat ttaagcaaga gggtatcacc gttaaagacg
    26581 atgggcaaac ttttgaatat acagctacaa ataatttgaa aaaaggtgta gtaaaagttc
    26641 ataaagtcga tcaagatgga aaacctttag cagatgcaga attcacctta acggatgaaa
    26701 aaggtgtaaa agaagttaaa aagacggatg caactggtct ggctacgttc aacattattg
    26761 cgaacaaaac ttacaactta gaagaaacaa aagtaccaac aggttacaca ggcgcattca
    26821 aacaagaagg aattaccgtc aaagatgatg gacaagtctt tgaatatact gctaaaaaca
    26881 ctttgaaaaa aggtgtgatc aaagttcata aagtggatga tcaagggaaa gcattagcaa
    26941 acgcagaatt cactttgaca gacgacaaag gtgtaaaagt tgtacaaaaa acggacagca
    27001 atggcttggc aacatttaat attgttgcca acaaaacgta caaaatcgaa gaaacacaaa
    27061 accctaatgg ttatacaggg aaatggtcta aagaaggcat caccgttgct gacgatgaac
    27121 aaacctttga atatacagta attaatcacc ttgtacctga aacagtaaga acaggcggaa
    27181 tcggtacatc agccttcctt cttgtatttc taattgcagg attgagtatg gtgctactgt
    27241 attatcgaga acagcacaaa gcaaaataaa aaagtgttaa tggaaaagaa gtaaatatga
    27301 aaaaagaagg ctgacgtatg aaatcaaaaa aaattttttc aaatttaatg ctatttggta
    27361 ctttgctagg agtgggattg ggcgcaagct caatctcttc tagccttgga ctaggggggc
    27421 aagtagcata tgcacaacaa aactataatt atcaaggaac tggaacgatt catatccata
    27481 aagtaacaaa tactaatggg gatgggaacc ctgtctcaga tattgataac aaagggaaag
    27541 aaatagcttt accaaatggt gtaaaacctc ttgaaggcgt tgagttctct tatgtgaaac
    27601 ttactaaaga tgaagctaaa tccttgggat ggacggatag tgatactaca aaaggtcaac
    27661 atattgatga tgctaaagca cgattattac taaacaaaaa atacagtgaa gtaagtagaa
    27721 tgaagactga ttcacaaggg aatatagaca tacctataca agtaaataca gacgatttaa
    27781 cagataactt gtatttgatt gttgaaacag gaacagtcaa aggtgtagtt gaagaagcgt
    27841 atcctatgat tgttaacgta cctgaactaa acggatcaga tgatcaatac tttaattatg
    27901 atgtacatgt ctatcctaaa aactatttcc aattaaactc agcagagctt gataaaaaag
    27961 acgaagatgg aaaggctctt gccggaactg tttttgaatt gcgaaaagat gatggatctg
    28021 atcaggttgt tggaacttat aaaacagaca gcacaggtaa attaactgta gatggtttgg
    28081 aattaggtga ctattatttc ctagaaaccg acttgggaac agatgctaat aaaacaacct
    28141 atttactaga tggtaaaaaa ttcaaattta gcgttaaacg tggggagact gctaaagtag
    28201 ttcttgatgt taccaactac aaacgacctg taattactaa ggtaattgat aaagaaaccg
    28261 ttgatactgg cgaagatgtt caatatacat tgaaaccatc aattcctggt aatattggta
    28321 attacgaaaa atatgttgtc aaagatgttc taggtgcaga gttaacttat gattcatcag
    28381 aagtgatcga aggaatgact ttagatactg attacagtat cgaacgtgac ggacaaacga
    28441 caaactatgt ctttactgaa tcaggtatgg ataagcttca gaaacaattt gatgcagggg
    28501 atcgcttctt tacaattttg atgaacgctc atactaatag taaagctaaa atggatacac
    28561 cacttaagaa tgatgtttca acgatctaca acaatggata tgaacccgaa gacgaaacac
    28621 cagttgatca cgcttataca atcattggtg atcaccaatt cattaaaaca gatggagata
    28681 caagcaaagc attgagtgga gcaaaatttg tagtgaagaa caaaattgat ccgaataaag
    28741 ttacttacct aaaacaagat ccaacaacga aagctgttac ttggattgca gataaatcta
    28801 aagctactga attcgtttcc ggatcagacg gtaagttcca aattgaaggc ttgcaatata
    28861 cctatgatgt agattttgat aaacaaggta atccaacaat cacagataaa aaaatcaatg
    28921 attatgctct tgaagaagtt gttgctcctt caggatatac accaactaga aatgatgttc
    28981 catttgtggt atcgaatgat gatgcttcta gcgaagtaac agacatcaag aaccttaaaa
    29041 cacctgaaac agttcgtaca ggtggaattg gaacagtagt atttctagtg gttgcaggtg
    29101 tggcaggaac agggctatat gttgtctaca aacgagaaca acacaaagct taatgaaata
    29161 aaattcccta gagtgttttt ggctctaggg tttcttttta aaaagtgtgt gaaaagtagg
    29221 tatgagcatg aaaaagaaga atatcaagtt gatcatctta aaaattggga tgttgctatg
    29281 tttaggagta ggtctatatc ctgtaattac tcaggcttat acctactacc aaactcaaca
    29341 aacactaaac tcctataagg aaaaatatga tgaagcttct gaaaaggaga atgaagaagt
    29401 cttcaagaga tattcagaag ttaataggaa acgaaaagac acagaacaaa cctcagtcac
    29461 gattcctgaa gataataaca atattccgtc aaatggaaat cttcagaagg ataaattagg
    29521 tgaattagtg ggtgaaattc ttataccaaa gattaatgtg gatttgccaa tttatgatgg
    29581 tgtaaacgat tttcaactat cagcaggagc aggaatatta accggaactg attatccaac
    29641 tggtgggatt ggaaaccatt ctgttatatc agctcattcg ggcattccta ataaaaaata
    29701 ttttacagat ttagagttgc tcaaacttgg tgataaattt tttattgata tttcagggaa
    29761 aaagcttgct tatcaagtag atcggataaa aataatccta ccaaccgaat ttgaggattt
    29821 gcttcctgtg gcaggaaaag attatgtaac acttatgaca tgtacacctg ttggagataa
    29881 ttctcatagg ttactcgtta gaggggttag cataccttac caaaaaagta ttgttgagaa
    29941 agaaaagaag aaagaagatt taaaggtaaa ggcgcagatg aactggtatc ctatcgtagt
    30001 tgtaggggtt ttattaataa tagctgtgat agtcattatt aaaaaaatag tagcaaagag
    30061 acgaggaatg aaaaatgaag aataaaatac tacttttttt gactcttatt tgcactttgg
    30121 ttttaacaat ttatcctgca aatcagctct atcatcaatt caaaacaaaa gagaatgtgg
    30181 cggaaatcca agagaaaatt gagaaaaaac aaactccttc aaaagaggaa gtggcaaaaa
    30241 aagtcgaaca aataaaatcg aacagcggta accaaggatt agacaatact cagaaaacag
    30301 acggcggatc atttgatatt ggtgaagctg aaggaaaggt caatccttac acaggagatg
    30361 ttttttcaaa aaatcaaacg aaagattttg aaccatctac ctattcaggg gcggttggtt
    30421 atgtctatat tccaaaaata aatgtgctag agcttttgtt tctaggcgct tcacaggaac
    30481 atttatatag aggtgtggca cagatgagtg ctactagtct acctttatca ggtaaaggtc
    30541 atcaaacagt aatagcaggt catcgtggcg gttatacgtc accacaattt ctttatgttg
    30601 atcaattagt agccggagac cacatttata tagattatct tggtgaaaaa gcagtttata
    30661 cggttttccg aacagacgtt attagaaatt acggtcagaa ttcaacactg actgatattc
    30721 ctgatttaga tatgttaaca ctttatactt gtcatccata tcctcagaac tacgatcgtt
    30781 tattagttag aagtctaaga gatgaaaaag caaaatacat cacgccacaa gtaacagttg
    30841 gcaataccgc atacgctgat gccattgata gcgagtacgt ttcagataag acagaaactg
    30901 ttacatcttc taatgattta tttgaggatg ttgatcgtgc caacaaggaa ttagatggaa
    30961 ataatgatct taatattccg gaacaaggta gcgtagagat gcaagacaga acatttgctg
    31021 aaaaggcaag ttcaatattc acaggaatta ccaataaaca tcagaaaatg cttttagtca
    31081 tttggactgt aataggagca ttagcaagtc taaatgttct tattctttta atcaaagagt
    31141 ttcgaaaagc ataatgaaga ttataaaaat tgatagagta cagtaaaaag ggattgatac
    31201 tattattgat ctctttttta ttttgaatag agctagattc acttttaaat aaaatcaaaa
    31261 ctgatcttaa ttctaaagaa aaagtcaaga aaaaaataca acaaattttt ttatcagaat
    31321 atttatcaga atattattag aaaagataaa ctcaaaagct taatttaata gggttattgg
    31381 ttgtatttag aaataatgta tggaaaagct ataaagaaat agataagatg tagaaaatct
    31441 tatttgtgaa tgaaaaacaa gtcatcgtaa aatattaata ggaggtgtag taatggtggg
    31501 aatatcaaaa acagctttag actcattagc taaactattt agtagcaggt ttgatagtgc
    31561 agaatttgtt gaatatctaa gccaaaacga aagtggaaaa actctgatcg aaacaatgaa
    31621 aaaagactat ttgaatgaaa atgactttcc gacatggacg aatattgaag atgctacatg
    31681 ggatgtagtg tgtacgatat attctccttg ggtaacgatt gctgattttg ttttagataa
    31741 aattggaggg gatacatacc ctgatgccaa atctcttgct acaagtgact ttaaaacgtt
    31801 ctatggttat ctaataggtc taataggcaa tggaatagat ttgaaagtta cgccaaaaca
    31861 aacacctgaa gaaattgaaa aagaaaaagg agaggttcaa atggcttatg caaatgacag
    31921 agaaaaagta aaattattgg aaggtaaatt atctgaagaa gaacttggaa aaatggtgca
    31981 agaaatcaat gaagacagca actattttcc tgaattgaag gtatatcaaa atgacgaaga
    32041 tttctttaat gaaatgttta gtaatagtcc atgggaagca gtgagtaaaa caaatttagg
    32101 agaatggaac tttcaagatg atctagttgc atttagcaat gatgggtatt tacgatcata
    32161 ttcagaaagt gagtatagag ctgaattaaa aacgtatgaa acggatattc tgatgagcta
    32221 tttaaatcta ttagagagtg gtcagggaga gttaagagat aaagttgaag aactattaaa
    32281 agaagagcta tagggattat ttcaacataa acacaagcag aaaactacca aacaaaacgt
    32341 accatgcaaa aagtcggtta agccggcttt ttgctctatt atgagaggag atcaaaccat
    32401 gagtgcaaca accgatatgg ctttagaagc agtaataaaa gataatacag gaaatgtcga
    32461 aaatgtagaa gactttagcg atattgcaat caatctttca gcatggattt cagctgtagg
    32521 agaagaaaat atcgaatttg agatcaagaa tatggatgac ttaaaagcaa aatttccttt
    32581 tatcgcttcg aaactaagtc aatttgccga ggataaatcg tggaatgaat tagttagttt
    32641 ttccgaaaaa atttatgata ttacgcaaga agatgagtgt gtttcatggg aaatgaataa
    32701 tcaatacaag aaaattgatg ctttcgtcaa atcacgtata gaaggaacag cttttgagtt
    32761 tgaaggtata gataccgcag aaaattggtt aaataaagaa tcctttatta aaagtatagc
    32821 ggatgatctt gaaaaacaat ggtctaagga agactatgca gagtacctaa aaacagaaag
    32881 agatacagag gtgaaagaaa aactcactga ggtatgtttg gatgaacata ttgatcccaa
    32941 tatcgtaaaa caagaagact atgaagtggt tattgataaa gttcctactg attttgagga
    33001 agttgtaaag tatatagttg aagaagcatt atcgcaatac tcttcagatg aggaagtgga
    33061 cgaagaaact gttcgtacca gagtttggca actggttttt gatgacaaag attataaata
    33121 tttgagtgat caaaactcgg ttaattggag tgtacgatta ttaattgatc aaaatgatat
    33181 tgaataaaat agagctgaaa agctctattt ttttgataaa aaaactagct attttattaa
    33241 aaataatttg gttcaaaatt tattttttta acttgataat tttttgtcat ccttttttaa
    33301 attggctata tatcaaggtt tatagtggga acaaataaaa atacatttga caaaatttac
    33361 aatatagttt attatatatt tatcttatta tgataacaca ataaaaggaa tgatggtatg
    33421 aaaaagaaga agtttagtcg atcgttgtta ggaattttgg ttttagttgc ggttggtctt
    33481 agtggcaagg gttactttca gaataacagc actcaggaac atgattttca gatcaaaagt
    33541 agtgttcagg taggtaataa agcagacagt gaatcaaaag aatttttagg gatacgttat
    33601 gatccaaaca aatatccaaa taatatcgta aacattaatg atgggaaata tacactgaca
    33661 aaagaagacc atgaagcatt atcagaaaaa ggcagtcaaa ccttttgggt aaattatgca
    33721 gatttagatc aacttggaag agcaggaaat gttgaagcgt tgatcaccta tgatgctgta
    33781 aattctcact catctaaaaa tgtggaacgt ccatctttca aaagttctgt ccatattgca
    33841 ggagaataca aggatggagt atttgatcaa gagagagata cttgggtcgg caaagattca
    33901 aacaatgaaa tcactcagct agaaggctat aaaggttatc tctataacaa aagtcattca
    33961 ttagcatggt cgctaggagg agatatggag gtacacaatt taacacttgg aacgagagcg
    34021 caaaatgttg ggaaaaatga tcagaatgga gggatggctt actcagaaac gattgtacgt
    34081 gatgcaatct ataaaaataa gaatgaaaaa gtattgtata aagttgatcc aatttataaa
    34141 gggaatgaat tggttcctcg tggttctcat gtcgaagctt tttcagttaa cgataatgga
    34201 aaaacggtaa accttaatgt ttatgtattt aacaatcaaa aaggaatcaa catcaactat
    34261 caaaatggta cttgggtaag agattagaaa ttgagaaggt gaagaattgg aaacaataag
    34321 catcaatgga ttagataaat cagttgcaaa tcgaataaaa aaaggaaatg gctactatct
    34381 tctgaagaag ggtaaatatt cagttgctag agagttagaa acaatgagta tcgtgtatgt
    34441 agctaagaat cattttgacg tagaacaggc tatttttgat ccagattctg aaatagggaa
    34501 atgtgttgaa ttatcagaga aaactgttag tacaaaaggg gcgatgatca ttgagagaga
    34561 aatgttagac gaaatcagag taatcattcc taaagatacg cagatgtgtg aggacagcat
    34621 aagtgacgat taaccaaaat cagaaagaaa ataaggagat attcagatga gcgaaaaatc
    34681 cattgaatta aaggaatata agcgaagcga tggagaaaaa tattatcctg tagccaattt
    34741 ttctaaatca gtatatctgg ttggacgtgc attggtagtt agtgcaattg gcatatatat
    34801 tttgcattgg gtaacaaagg ttgtacaact gcttgtagat accttagtaa gtattctcgt
    34861 ctcgttaaat gatgttaacg gaacagatga tttaggatta aaagagatgt ttaaagagtt
    34921 actcaatgcg ttttttccaa attatttaag ttattcatat aatgtacaaa gcttttttgc
    34981 aaagctacac ttggtagata tctctatttt agtattagaa gcaggaacgg tattctttgt
    35041 cggatatttg gttactttga tgttcaggca tcactatgga gaacaaagac cattctttga
    35101 tgatatggaa agtagaaggt tgaaaagaga agctctagaa gctatggatg tgaagtatga
    35161 tccaaacaag attataggag atgaaacgaa caacaaaaaa agaaaacgaa acaaaacgca
    35221 attagaatcc gatgtgctat caacgcttaa aaatatcaaa atttctgttc atacaagacg
    35281 tgatctaagt gtcaatgaag atattcgaga atatatcgta aaaataccgc aaccaaaagg
    35341 gaagaaagtt agagaaacag ttttacgaca aattcaagat ttagggtcga tcctgacaag
    35401 agcaacaaga gggcgagcta aatttgggga aaaagaagac gaagtttcag gtgattactt
    35461 catcttcaga gcatcagtaa acattgaaag agaacgtaag gaaagagtaa gtaccaaaaa
    35521 caaggaaacg gaaaaatcaa cagcatccat tgcagtttca aattcacaat ttgcttttcc
    35581 aatcaatctc tttgcagata actccaaaaa tatccaaaac caaaccgaaa aagctagaaa
    35641 atatgccgaa actcaaaagc aatctttaga actttatcta tcttctgctg acgtacctgc
    35701 aagtctaaca gatatgcacg ttgggaatac atctattgaa tatttgttta ctttatctcg
    35761 taccagaaaa attcaactaa gctcaatgaa agaagaaatt gaaacttact tgcatatatc
    35821 aggtgtaatc gtgcggatca acgcagggcg tattcaaatt actttaccat taggagaaaa
    35881 tgatcgtatt ccaattgatg taccatcaat gatcaatagc gtgttttaga agacatcaca
    35941 aatagaaaca gaaaatacaa actaccagtt cacaaacaga aaagtaccga gaaaagagag
    36001 gaatagaaat ggctatgaaa ttgttagaga aagtcaaaaa tagagccgaa aaagctgaat
    36061 ctattttgct agataaagaa cctaatataa agcttaatgg aaaattcagt gtcaatatgt
    36121 tgaacacaaa ggctaaagac ccgacagatg ctatttttgg aattaagcct gatggcacaa
    36181 ttgctcattt ttgcatcagc gatacgccac acatgttagt tgccggaaca acaggatcag
    36241 ggaaatcggt attactgaat gaaattcttg ttacagctat ctgtcatgca atgcctgatg
    36301 aattaaagct agggatagta gacccaaaac gagtagagtt tgggcgttat aaaaaattac
    36361 cctatatgct tgctgatcca attactgata tggatgaagc ttatgaattt tttgaatacc
    36421 ttgtgatctt aatgaatgaa cgctacaaat tgatggaaaa agcaggcgtt caaaatatca
    36481 cacaatataa caaatacgct gaaaaacatg gaatagagaa atttccctat gtgatcttat
    36541 tggttgatga atattcgcaa ttagttggaa cacacaaaga agttgaaggg ctgatcgttc
    36601 aattaggaca aatggcaagg gcggcaggaa ttcacgttat tcttgcaaca caatcacctc
    36661 gatctacagt cgtgacaggt attattaaag caaatttccc atctagagtc tgcttgatgg
    36721 tagcaagtga tttagaaagt aggatcgtat tagatgaggg tggtggagaa agcctaagtc
    36781 caaagggaga tatgtttatc aagttgaatg gaggaaacaa aatcagagcg caaggagcct
    36841 atatcagcaa cgaggaaatt gaagcgatct ttgatcacct tagaaatact atgccagaac
    36901 cagaatatgt tgattaccga gcaatcgtag ctgaatctcg tggtgagggc ggtgaagacg
    36961 aggattttgg tggaattggt aaagctcctg taagaaaatc tgctaaaaga aatttgaaca
    37021 cacctttagc aaattcatca tctaaagagc catcaattga aaagaagagt acgtttgctc
    37081 taaaacttga aaaaaacaaa gaagaaaaag aaaaacaagg agctaaaaca ctagaaaaac
    37141 ttcaagccga aaaattagaa aaagttttaa aagcattaga taaagttgat cctgaaccgc
    37201 aaacctcaaa caacacgaat aatgaacagc cgatttcatc taaaactaaa ccgaaaacta
    37261 ctgatccgaa agtttcagta aaagtgacca tcacaaccga taattctgag aagaaacaag
    37321 taaacggaac tacttcacct atcatgcaaa aaatgttaaa agccaaaaaa caggtgcata
    37381 aatgatcatt gtcagttctt ttttgaggtg gcttacgcca tataaatata cagatgaagt
    37441 agttgtgaaa cgagctaaga aaaaagataa aaaagcggtg ttaactccta aacagatgct
    37501 tgatcatgga attttgggtg tgaaggtttt agaagtgaat aaaccgtttg aatcaaatag
    37561 tgcaagagaa aagttgcttg gcgctgaagc agatgtggca tatttttatc atgacaaaag
    37621 caagcatcaa atagctatta aattcaatcc aaaatcggca tggcggtatc aagcaagaga
    37681 tgacttgaaa cgtaggaaac ttagaccaat gctctatcca gaaggaaaag aagcatttga
    37741 tagaacccac atcgttccca tcggttttca tggtacagaa tcagataatc gcctattagt
    37801 aggttggaac agcaatcaaa atagagttca gatcaataaa tttgagaaga aagtaagaaa
    37861 attcaacgaa cgaaaaacaa tcctatggtt tgcagatatt tgtaaacagc cggacggaac
    37921 agcaaagtgg gaagcggtga tatttgacga aaatggtaaa aaagtaatgt ctgaaacatg
    37981 gcatgataaa gctaaatttg tttggcagta gaggtgaaaa ttgtgttaac tattctaaaa
    38041 ctatatagag agggatttaa cgaaaaagta agtattggtg aatccattga atatagaggt
    38101 gataagtata ttattattcg cattcttaaa attaacaaac attattttag caaatctatt
    38161 agtttaacag ttagagttct agttcagaaa gtcggagact tgagtgatta ttataactgc
    38221 aaaagagaag ttattgtgga taatcgatac gaccaatcaa aagatgataa gcgaataata
    38281 aaaataggag atattgtctt tcctgagaaa gggatagcat atgaagtagt gtctatcaat
    38341 agttttcgct atgaatttgt tgatttggtt gtcgaatatt ctgttcgatt gatcgttccg
    38401 tggagtaaag aagaaattaa tgaagcatta aaagatgaac gaaaaagtac attcaaagtt
    38461 ttagaaggtg aaaaaaagtg aaaacagacc ttactagaca agctgagaag tgtttatggc
    38521 atgaaacaaa tcgcatgggt gtatttggtt gttttgaagt aactatcggt tgggatggca
    38581 gagaaagagt agattatata acttattcag tagataacac agttcgatgt tatgagatca
    38641 aagttacttt atccgatttg aaaagcaagg cgaaacagac atttatcggc gattataact
    38701 atttagtcgt tactagtgat ttgtgggaga aaatgctaca agacgacaca ctcatgcgta
    38761 aatattataa acaaggcgta tacgtgttta atgaaaaata tcctagcatg ataagtgtta
    38821 agaaagcaag gaaacaaaaa gtttcgctag gtacaagatc aactgtatta gaaagcatgg
    38881 ttagatcgat gaatcgtgaa gttaagaaat tatataaagt aaaaccattt tgggagatag
    38941 tagagatacc ggaatgatga gtaaggaata ttatatctat tcatatatag tattaactga
    39001 ttatgaggag gatacagcaa atgaaaaatt atgatccgaa tatgcgatgg ggtacacata
    39061 ctcttaaagt gtcttttcaa cggaggataa gctaaatgaa taaaagaaga atactgtggc
    39121 ttattgcagg aatgtatctt tgcatagcag tatctgggtt tactatttgt cggataataa
    39181 atcagttaaa aaacgcgatt gtttatctag gagaaattgg ttatctagaa gatttaaagg
    39241 caggactaca aataatgagt tggattttat tcgttctgac agttgcgtta gttatattaa
    39301 cttgtgaaaa tttttcaaca ataaaaaaac aaataatatc taaccataat caagagactg
    39361 ttaaatcagg tctagaagta gacccagaag aggaagtaac tgaacaagcg atcaaagagc
    39421 aggaagaaat agacaaaaaa gaaataatga gtatttattt atctagtgga gagtcatttt
    39481 ttcatgaaat agattcgctg aaacttagta gtcaagaaat aattgataga tggcaaaata
    39541 tgtctttaga tggggagtta atcgttgagg atagtgaagg ttggtcagct tataagaagg
    39601 acgagattgt agctatcacg attttgtttg tatagtaata tataccacta gataatctga
    39661 atatgttgat atgctagtga cgtaccaaat gtgttcaata gagccgtggt cacaggcaga
    39721 aattgataaa atagtaaaat ttaaccgatt atcaaaattt aacgttttag gaaatacctt
    39781 ttaaaaatca caatacacaa acagaaaaat accaaaaaat taacaatgaa cgtaggtaag
    39841 aacatgacaa taaatgataa taacctcaaa aaatataatg ctcttgtcca acgctatatc
    39901 gaaaacaaaa taacgatgca agaggtcgct gatgaattag gcaaatcaaa aagcctagta
    39961 tcacacgact ttaagcacaa tgtttttccg aacccgattg agctgaggaa aaaatttcaa
    40021 aagagagttt atcaagaaat agggaagtgg attgatgaag ggaatgcaat tgacccttta
    40081 attgaaaaat atcctttttt aaaaagcaaa aataaaattc aaaggttaat tcgtcaaggc
    40141 gatattcaag ctaaagaacc tgttttgaca accaatgatt tggctaaaat aaatcttcat
    40201 aaacaaatta ttgaagactt tgaaaaaaat ctttcaacaa ctgaaatttt aaaaaaatac
    40261 aatattccga gaggaaagct aacgtcgact ctttttagag aatatggaga atcggcagtt
    40321 actaaagcaa aggaagtagc gaatgagaga aaaaaacaaa aatagtagtc gatacttagt
    40381 agcgactgta gtttttgttt gtgtattgat tttcctgttt gattttttac agattggaaa
    40441 aagtataatt ggaaatacat tgagtgcaaa ggaaatggtt gctttaggtg gtttgtatga
    40501 aggacatttg ttcccgaata tattcatttc tatcgtttct catattaact tgtaccattt
    40561 aatttctaat ttattgttct tagtagttct tgaagaagca ctgaatgatc agtatgagtg
    40621 gttaacatta ttgatcgttt taatcatatc aggatttcta ggaagcttag ttccttttct
    40681 gctacaaagc aacatcgtaa gtatggggct tagtggaggt atttttggat ggattgcttt
    40741 atttgcgatt gcatacatcc attttgatag caaggaacat ttattactaa gtgtttttgt
    40801 attattgata atcggcattt tattggtatc tacttttgaa tcaggcgagg caaatgtctt
    40861 gtcacatgta atcggatttg tctgtggaat gatcttggga tttattctaa agaacgtttc
    40921 attgatcaaa caaaagaatt aaaagggagt gctaaaaatg ccaaaaagaa aagaaaagaa
    40981 aattccagtg tttttgtctg tctcagaaat gttatcactt caaatactgt taagaaaaaa
    41041 gattgaaaca atagacgaag aagttagaaa taaaagggct atgcacgaac aacgtgaagc
    41101 gctaagagaa cgaatccact attttgatga tatacacact aaaatctatg aagcacgtac
    41161 aaatcacgaa gacgaaagta aattaggatt agctcaacaa cggatacttg aatggcttaa
    41221 tacgaatact gatcaagaac cgttaagagt gattagtaat ctttttgaat ttggaacgaa
    41281 gcattggttt aatgctgtag agaaggatgc tgacttacaa gaagcgtttg atacgctaag
    41341 taaggatagc aaatccatgc gagatttatt gattacttat ctaaatgagc atgagtttga
    41401 acaaacgaaa accaaaaaga aaaataaaga atagaaagtt gtgatgaatc atgctattaa
    41461 ccctgttagg ttatttatgt ttaattcttg ctttttttgg ggcaatcgct ataattattg
    41521 gtcaatttca aacaaataat ccgattatgc gtaacatagg cattggaatt gtgattgtaa
    41581 cctttattgt gtttaatgcc actaagattt ttggaagtaa cgataaacaa tttggtatat
    41641 cgggagttaa taatagttat gctccagatt ctttgggaag ggtagtaata gatggaacat
    41701 ttgaaaatgt aacgagttat aaaaatgttt cgctgacagt gaaggaagat ggcaagaaag
    41761 catcagacga cgttctcgct actattaagc cgtctcaatc acaatttgaa gtgttttata
    41821 cagttaaaga gaatagccct gtacacaagc tgacattggt ttttaacgtt gatggagaag
    41881 aatactataa agatgtaaaa ataaataaaa catcaacgaa aaagattatc gaatcagcga
    41941 aaaaagaagt agctacagaa gaaagtacaa ctgaatcaag ttctactgtc gaagcggaac
    42001 taccagataa aattgatttt gatcctgaaa aagtcaaatc gtttgaatct gtttctggtt
    42061 ctatggctga tcggtttaac actgaagtct tcaaagctat caaaactgtt aaattggata
    42121 ctgaaaagca aatgattaat gttaccttat tcaattcagt tgaatctatg gatcaggata
    42181 aaaaagttac ggttgcaaga gaactaggaa aaagagtaat tggtttgtat cgtggtactg
    42241 ctaaaccaga ttcagacggt gcagatttgc cagttcatgt agtttatgaa gacggtaaag
    42301 tttttggaga ttctacagca aacgaagcat taacgattaa attggctaaa tagccagaaa
    42361 gttataataa acaaatggtg tcacaccaat aatatatagg tgtgacacct tggagatgat
    42421 agctgatggt agtaaataaa gaaaaagtta gaaagtatgt gactttaaaa atagcttctg
    42481 ttaaatattt agaaaagcga gctaaagaag aagaaagagc aagaggtaag aaagtaacag
    42541 tttcagagtt gttagaagaa ttgatcgaga atgatcaaac aataagacgt gcatttacta
    42601 ataggtctat cagataacaa tataattaat aaaaaaagaa agagtgattt ggtcttagac
    42661 taaatcactc tttttatata aagtttcttt acactcattc cgaataatgt taaaattctt
    42721 tatacgatat atagttcctt gtcagaggaa gagataacca gcaatattgg agggataata
    42781 agtggaaaac aataatgata ttagacattt tagcattggg gaaagagtta caacagtaag
    42841 tgaattatca aaggattata gagataaaga gggaacgatt atttctctta ataagaatcg
    42901 aagaggcgtt tggcaatatg ttgttgattt aggtgatggc gttacagttg agtgtaaaga
    42961 tagtcaactg atcagaaata atggcagtca ttccattgaa aaagcaattc ttgtataccc
    43021 aaaagtatca agtaagatca tgaatgactg gttgcttgga aaatttggtg agtacaataa
    43081 aaaagaaatc gcagaagtga tcggcaaatc ttatccaaca gtattagggt atctaaaaaa
    43141 gataacgagt aaagaagcaa aaattgagga tgttttaaca gtaagagagc ttcaatcatt
    43201 aatcgagtat cggaataaac aaataacaaa tcctgagtac cttagaaaag aagatggtct
    43261 attgaaacaa ttggaacaag taaataaaat ccggaagatg caaaaggtag ctcctttaag
    43321 ccttgatacc tttaaaaaaa tctatttgaa agagatcaaa aaagactaat gtggactgta
    43381 actttaccgt tccaacctgt atctgcacca agaccaagtg caagaaggaa tgctgctgat
    43441 gaaactaatg acgtagcaga gaaaaggatt tcaacctact atgataaaaa gtatcttgat
    43501 tatttgaaaa aaataaagag ctttatcgaa gatcaagatt tattagatga tgaattttac
    43561 tcgattgtaa atgatcctat gggcgcaatt atggaagtgg acttttacta ccagatacca
    43621 aaaagctata aaaaagtcta taacattatg aagacaacag cgccagattt agataattta
    43681 gtaaaaagtg ctatggatgg tatttttaac atctcagcta tacgagatag ccatatcaca
    43741 ggcttaattg cttttaaata taatgtggcg aacgaaggaa aaacagttgt gaggatcaaa
    43801 cgatattcag aatttgagtg ggatacttct gaaggattga acggagatat atgggaagct
    43861 acatttgatt ttaaaccagt agcaacgcca agaccaaagt ctaaatttag aggaaatggg
    43921 attatcactt actatccaaa agagtacaac gactatttgg aaaatatcaa gaatacgctt
    43981 aaagagaaag atttggtaaa tgatcaactg aaaaaggtaa tgaatgctcc tatgggggtt
    44041 atagctcaga tagattattt ttaccaagtg agaaaagata agaaaaagct tgattcactg
    44101 atgcgtactt cacagccgga tattgataat ttagtcaaag gaacattgga cggaattttt
    44161 aacaaggaaa taggcatcaa agatagcagg gttgtagggt taattgcttt taaatacaat
    44221 acttttgaaa agtcgaaaac aaacgtgaga ctacaggaga tgggctgatg aataataatg
    44281 aaaaaattct tcatgtactt gatagctttg aaataattca agaagaacta aagaaatatc
    44341 gggatgtttt ggaacaaaga tatgattttg tcaatcagca aaagagtaat cacatggatt
    44401 tcattctcaa tatgaatgac ttgaagaaga aattagttga acgaaaagaa caagaaaagt
    44461 tgatcaaagc ttactttgaa ctcggtgaaa aagaagttaa aaatgcaatg gaacttaatg
    44521 aagagcgcag ggttttagat cagctgttag aacaattatt ggtgatgttt caaaaaggac
    44581 gaatagatga ggatttaatt gaagaaggat tgagaaaata tccttcaaac tcaggtatcg
    44641 gaatagtgct gaaagcgatt gatgaagaag aaattgaagc tttcataccg ccagaggatt
    44701 ttgaatcggc aatggaatat ataaaatatt atagccaagg gatcactgct tttagagagt
    44761 ttgatcctga agatgttatt catgacttga acaacctaaa agaatggtgt gaaagttatg
    44821 gagtcgatga ttccggattg gactacttga tcagtattat ggaaatcgaa gaagagatgc
    44881 cggataagcc tgatccaaca gatattttag aattgatcca tgaagctaga aatccaattg
    44941 cctatatttc tagaggatat actgtattgg aatactataa gccttatata tcagcgatga
    45001 accatttaag acgtgtatta agggaaaaac gtgaataccg ctcagtgttg aatgcaacga
    45061 atagattaga aaaagctgtt tcagagctag atgcttatta tcgtgaacat tacctgcaag
    45121 caggtggtat gccaagaaat acgaaagcaa atatcagtag gtatataaaa aaagctgagt
    45181 aggcggagtt agcccataaa atggcaaaag gtaacctgaa aggggcattt tgaatgaaaa
    45241 gggatgtttt tgaattgtac gtattagaac gtctagaaga cggcatgatg gcacaagaga
    45301 taattgatga aattgatgat atgatcagca gatttggtca attggagttg aatgatggaa
    45361 acaaaaattc ggtagtgcta aaaaaatttt ggttgaacaa tttttcgtat aaggatttta
    45421 tcaaagaaaa ggcaaataca ttgtactgac taaattcacg aggagcagtg atgagagctt
    45481 attgctaaga gcagttaggc tcttttttgt ttgtgtaaaa ttatttgttt tataatacac
    45541 atttatacac aaaaaaatat tgacggatag tacacaaatg tggtaggata tatttgtaag
    45601 caagttagat taacaagtta aaggaaggaa gagcagttat gaaaaaagaa gaactaatag
    45661 atatatttca aattgttgaa cgagcaaata atatgggaat tatgtttttt gatcgtattt
    45721 ctttaaagat ggatttaagc gttgcccacc aagaatttaa tctgcgatta aaagccttgt
    45781 tgaattctga tgatgttaat ttcgctcatg atgttgtagg tatacagaac catatagata
    45841 gagaaaacaa acgtatgggt gatgaattct taccaagata ttcaagttta tgatttggcg
    45901 aaagattcaa gagtatgtgt cattattgat aggtactctt gaattggaaa ggaggaatga
    45961 atttaagaca acaacgatta tgatcttaaa acaatcgttt atgtaattgg caatcttacg
    46021 aataattttc atgcaataat gacaataaaa aatgaaaagg ggtaaaatca aatgaaaaaa
    46081 tttttagtag gaattctatt cacaatgggg ctagtattat tatcaccttc agtttcagca
    46141 gaaagctcta tttccatgta tcgtatgtat aatcccaata gcggagagca tctatacact
    46201 agaagcgttg gagaaagaga caatctgaaa cgttatggat ggcattacga aggtgtcgca
    46261 tggcaagctc caacaagtgg tactcctgtt tatcgtttgt ataatcggta taacggtgaa
    46321 catttttaca cagttgatgc cggagaaaga gataagattt cacacacagg atggacgtac
    46381 gaaggtgttg cgttctattc ctacaacgga gcaaatagag ttcctgtaac acgtttatat
    46441 aacaaatacg tgaattggca tcactacacg ttagatcaaa atgaaaagaa cgtaattagt
    46501 aaacaaggtt ggaagattga aggtacaggc tggtatgcaa tgccaaacag tgtaccaaat
    46561 cctacacctg ctccaagcac tgaaaaagtg ttgaacgttc ctgttgtatg gcaaggaaat
    46621 acaatgttat gcgaaggagc ttctttatta gaaggtttac actacaaagg tgtagccaca
    46681 aatcaaagct taaccagttt tgtaaactat atgccaaaag ctaatgacaa taatccttat
    46741 catggttact caggagagtg gcgtcataac gttaatggaa cgtatcaagg catgatggct
    46801 gacccagtag ttaaatgggc aaaaaacgtt ggcggtaatg ccgtcaatat cactggttca
    46861 ggaacgaatg gaatcaagaa tgaaattcgc aatggtaatc cggttgtaac ttggattact
    46921 tataactatg ctacgccaga gttcaaacaa atgccttggg gtcgtgcagt atggaatgga
    46981 catgtagttc tcgtggatgg cttcagaaat ggtgcatacc atgttgttga ccctgtattt
    47041 ggcgcaaaat ggatcaatgc aagcacgtat gagagagcat ttaatgtgac tggaatggcg
    47101 gttgctgtac gttaattgat agaattatca actaaatgaa ataagagctt aattactgaa
    47161 atggtaaata ggctcttatt tttttagagg aaatttttgt aatacgaatc tataacaaaa
    47221 aatattgaat gtagtacaca aaagtggtat attaaaatcg tagagattat gcgaagaggt
    47281 gattttgatg aaccaaagaa gaagaaaaag gttagaagaa ataagcgagc gactttccga
    47341 gctaaaggat gagttagaag taattcaaga agaagagtac aaagaaaata tacctaaaaa
    47401 tctacaggga tcagaaagat atgaaaaagc agaagaatcc gttgatcaac ttgaagaagc
    47461 tgttgatggt ttatccgaca tattagaatt gattgaagat atagctagct agagatgatt
    47521 aatgaagatt tcaggagtga gaataaaatg attgatattt cactgataga gaagttactt
    47581 ttctcaaata agtacaccgc aaaagagctg gctacaagat ggcaaatatc cgaatcagct
    47641 attaaagcct atcgtcaaaa taagggaagc gtaggatggc gtgattggaa agggatcagc
    47701 attggaaaag cagaagacat aatgaaacgt gaaggacttc gatcctatac cacttctgaa
    47761 aaagacaaat aacttaatgt ttgaaaagat aaaatatctg tcttaaagct atgtcaaatt
    47821 caaatggtga atgtttgcta aatataatgc aaaaaaagga agggaaatat taaatgataa
    47881 gcgaaaattt gtcttatgag aataataaac atctagttaa agaaaataag aaagtagaag
    47941 attataagaa catgtatata gaaatgaaaa aatcaccact gtgtattcaa tataaaaatg
    48001 ataattggaa gcaagtagtc gaatttgtaa aacctcatag tatacgaaga gtacgaaggg
    48061 ggaagaaaaa aagtgaactt gtaaatagcg gaagagcggt aattagagtt ataaccaaag
    48121 attatccagt tgaatatgga gattatattt taaggttaag ttatcatgct tttgttgtgt
    48181 tgaaagaaga gcatttcaac gagtttttca atttaaaaag taagtaatca tatttttttg
    48241 tagacgtgta taaagttaac taaattgcga ggtgatgaca tggtagcaaa aaagaatact
    48301 ctaaaagaag cttctgaaaa atatcaagat atgaaaagac gaggattaat accgaaagac
    48361 aaaaaaggta tatggagaga atttgttaag gaagtcagta gtgatcgaag tggcggtgac
    48421 tttgttggac gatatacagc aaaaggctat agcaaacgat taaggatcac tgttgaatat
    48481 ggaacgaatg aatcagctac agagctgaag caaaggtact tagatacacg aagggaagct
    48541 tcaaaaaaat ataatgattt caagagcaga ggtctgattc ctaaaaagaa aaagggaatt
    48601 tggcgtgagt ttttcaagga agaagatagc aatcggaata agggagactt cattggacga
    48661 tacacagcaa aaggctacac taagaattta gggatcaagg ttgaatacgg aactggtgaa
    48721 agctcatggg aattgagaaa tcgctattta aatacattgt tagagaaata taaagcagaa
    48781 ttattggagt tgttcgatgt taatgatttt atcgagatgg ctgaaaagta tggtcatgaa
    48841 gaaggtttta tgatcaatga cattatggtt attgataatt ttacggattt attagcactt
    48901 gcgaaaaaag caagggattt aagagaaacg cagatgtatc tattcagaaa taaaaacgga
    48961 agaaaccgga gaactgaatc gagtgagatt gaagattact acgatgcctt tacacacgat
    49021 ctgggagcct ttgtagacga gataagtggt gtaatgaaca aatatagggt ctagaataaa
    49081 aaagccggag atgagcagat aaatgggaaa caatagcata tacacacctg aaatgcagaa
    49141 taaaattaaa agacttgtcg gattagggtg gatgacaaga ggaaaattag attcttttag
    49201 tacgatcaaa tatcttttga tggacattta ccgtcagaac aagcaaacag gaatacattt
    49261 tttgggttat gataagccgg aagatgcacc tttctttgtt ccaagttcag cctatagagc
    49321 cagtacctcc ggtagcatgt ataacttgga tttgtttgag gaaggagcaa agatgatcga
    49381 atatgatttc aacgaagcct attcgaggat catgagaaat tatcctttgc cgtcaaacac
    49441 ttatttgaaa gcaaaatata atacagatca agctattttg agagcgataa atagaccaaa
    49501 agtaagaccg tatgaaaatt taaaaacgta tatgttcttt ttggttaatt ttgaaggaac
    49561 agccaaagaa ggaacatatg ctaatccaaa atcacagtcg atgtttgcgg attatggcga
    49621 aaatcccaaa acagctaatg agcctatttg gttatcggag attgagctaa cacttatcct
    49681 tgattattat gatattaaag agatggaagt cctagatagc cacacatttt tgacgaaaaa
    49741 aggattactc aaagaatatt ttgaaagaat cgagccatta aaaactgatc ctgatacgtt
    49801 gaagctttat aaaaatctta gaacgaaagt atttggtgct atcggacaaa aatcattaag
    49861 agaaggagaa acaagcacct tcgataaata cagtatgtat aatcgagcgt attcctccat
    49921 ggtagcaggc gtatttagag atcgtatggt tagatatgaa cagaaatacg ttcatagcgc
    49981 atatgggcta ttaaaaattc ggactgatgg attgtatttc agatccgaag taccagagtt
    50041 tgaagagctg tataaacttg gagtagttaa gaaaaaagtt catacaatat ccaaagaaga
    50101 agtcgaaaaa gctttagaat ttaaaaacaa aggatttgaa aataagggct cctttgggaa
    50161 ctctaaagat gaagttaagg gtgttgtgat agacgatgat ttcgagtttg attttgacta
    50221 aatactcttt ggaaaagatg gtaggaggta aatatgaaga agaaaattgt ggaaggaaat
    50281 gtaggtgtta ttattctaaa agattcaact gactatgcta aatcaatggc actaaaagac
    50341 gaaaaagttg cacgcatgga taaagattta gttgaatttc tgatggatga tacagaaaga
    50401 ttaaaagggg atcagttttt gagtttaatg aaagacaaag gcttaaacac atatggttta
    50461 tttgattata agaaattagc tatagagtgg gtaaaagaag gagaaatatt ttttattgaa
    50521 gaatgggaag gtactgagaa cttagttctt catcagtatc ttttgaaagc ttaatctaag
    50581 taaaacagtt ctgactagtt atgcttgtca gaatttcaaa ggatgttttc cataaccatc
    50641 atcattcaaa aataaaggat ttgaaaataa gggttccttt gggaactcta aagatgaagt
    50701 taagggtgtt gtgatagacg atgatttcaa gtttgatttt gactagattt ttttgaaagg
    50761 tttaagagta aacaaggtaa gaactaagat catgaagatt gagagtaaag aagatagagg
    50821 acgaatgtat gagaacacac tatatttatt tagaagataa aattgtagat gaaattccgg
    50881 caaaaagtcg agaacaagct agaaaattat ttattgaaaa atacaaagag atcaatggag
    50941 atagtgaagt tccaaaagtg gtcgtaacag ttagaaaaag gcaaacaaaa aagagaagta
    51001 cgtttcatgt cagccctgat atatataatc ttatagagcg acatagaagt gaagataata
    51061 tttctatctc tcaatatatt gctacagtta ttgatgaatt gatttcagga aagtatgact
    51121 atgcttttga ttttgaggat gaaaaggatt acaaaagtgt tgctgtcttt caatctgatt
    51181 ttgagaagct aaaaaactat gctggcagga gaaatgttaa ttttgcaaga ctactagaag
    51241 agttctactc cgaagagttg ggagtaggaa atgaatacaa gagagaaact tatgcgaaat
    51301 tgtctcataa gttatcccaa gcgcttaaag aagtgacaga agttaaagcg gaactagatg
    51361 ctaaacttga agaagtgaaa aaatatcaaa cagagaggaa gaacaacatg aagaatcaag
    51421 tggaatttga cgaacagaaa gaatttgaaa aagcaagaaa acgctataac aaaattatga
    51481 atgaacttag ctatgaacac ttaacagttc atgaagaatt gagtgagggt acagaaaaat
    51541 atacgatcgc agatatggtt aatgaagtgg aaactctttt agcttctttc tttgaaaatg
    51601 gtcacgtaaa cgatgaatgg cgaaaagaag aacctaaaag ggttaacgct tatattgctc
    51661 gattaaaaag gtttattgat aaatataagg agaatagtga caaattttct aaagataaat
    51721 aaggagagag tgtgattaca tttgccagaa atcgaaggaa tattttcgcc aaaaataggt
    51781 aaaaaacagt tagatttcag cagttttccg agcgtaaata gagattataa tctaggtgat
    51841 gaggtacaga tactaaatat tgatttcaaa gaactagact tcgctgttgc tgtttccaac
    51901 aaacccaagg cattggaacg agtgaaaaaa atataccaag agaatgatca aaatatcaaa
    51961 aatatgatca aaaagtatag acgagataat tcaaaagttg aagacgtttt attcaacggt
    52021 acggcaaaac atgatttgaa tgtttatatg gcattaggag tggctttgta ctgtaattca
    52081 attaatcaga cagatatttt ggttaatatc gtgaggaata gctatcaaaa aatattaaaa
    52141 ataagtagcg aaacaaaccc ttattatgca atgaaatatc ttggggataa tttattaaat
    52201 gataaaaaat tgatgcacaa cactgaagag ctgttatcta atttggctgc gattaacttt
    52261 ctattcaaaa caggttctga agattatttt atagataacg tactaaaagg tgcttacgga
    52321 acagcatttt atccgcttta caatatgaaa cagcataaag ccgaacaaaa ggataacaaa
    52381 cttgaaatta aaaagatgaa agaactatta ggtataggat ttgtaaagcc caattcaaaa
    52441 gtcactattg cttcgttagc aactactaac aatgatggga aaatagtaaa tgggtttgac
    52501 gattcagata tagatatatt gtcaaaatca tatttttctt tgtatggaga ttttgcagac
    52561 ttgatgggta ttgatagtta cttagtcaat aattttgaga tcaagggtag cgagctgaat
    52621 gatattgtgt atttcgtttt aatctctcta tatgcaattg atcagaatac cgagtaccga
    52681 caaattgact atttgctagt tttttatagt ttgttatatc agttcgtttt aactcggttg
    52741 tttaaaaatg ctaaggaaga actgatcctg aattctgatt atgaaatagc aaaacaaatt
    52801 gcaagtatta gaaaagaaaa caaatctgaa gtaatcaaat taagaaaaga attaagtatt
    52861 gcgagtaaag aaaacggtcg gttagaatct ttagtcaaat cggcaaaaaa agaagaggaa
    52921 cttttgaagc aagagattaa agaattaaaa ggaatcagaa aagaatttga acagctgagg
    52981 tttgaaaata aagtattaaa gcaattgcag gaacaagaac atagacaatc attaaatggt
    53041 aaaactgaag agccttcact gtctgaaatg atagcttatc taaaacataa aaacattctt
    53101 ttagtgggag gacatgtaaa ttttcaaaat aaattaactg aactattacc aaatattaaa
    53161 atgtttgatg taagcgaaaa taatggaaag atacttaaaa atttgaatac gtgtgatgcg
    53221 atttttctat atacagatta cttgaatcat ggtctatatt atcgtgttat gagtgaaatt
    53281 tctaataaag ataagatatt ttatttggat aatcatgtga atatgaactt aacaattaaa
    53341 cgtatttacg atcttttatc acttatataa agagagaagt gataaatagg acacaaaatt
    53401 tggaaagtag gtaagtcact atgagagaac taactttatc ttctgaggaa ttagaaaaga
    53461 aaactgcttt attaaacgaa atatttagtg ctgattgtat aatccaaagt ttaaaagaaa
    53521 gttcattttc aaaactaaca gatcagaaaa aaatttctta tatgctaagt ttagataatc
    53581 taggaactaa atatgatgaa tcggcaagta gcgataagct tttgattgaa ttagtatcaa
    53641 gtcttagtag ttttggaaaa atgaaaagat ttaatgtgat attagaaatt tttgcttgga
    53701 taatcccttc atttatttgg tttttttata ttttcatagt tgaatctgag ttaatagcca
    53761 aggtctctct tgaactgtta attccgatat atgcatttta tttaataggt tcagcaattt
    53821 actttaataa cttgtataca cgtattcaaa taaaaaagca gttattacat gaaggattaa
    53881 gtggtaatat gttaaaaaga attgcatggg ataaagataa taaaacagtt attgagctta
    53941 aagaatataa atacaattat ttctcgagat gtatagatag aaagttactg tttaaaaaag
    54001 aaggattaga atttagtttt gatgaagatg aatttgaaaa attagtgatg gactggcgta
    54061 agaatttact ggatacggta caattctaaa agcaattgag gtcgaaattg cgatacttga
    54121 atcatggtct atattatcgt gttatgagtg aaatttctaa taaagataag atatttcatt
    54181 tggacagtca tgtgaatatg agcttaacaa ttaaacgtat ttatgatctt ttatatgttt
    54241 ataaacatta atgaggtgct aaaaatgaca acaataacta tgacaaaaaa tatagaagta
    54301 tatgttgatg gatcacaaga agaaagggat tccttttttg attttttttg gaaaaatagt
    54361 agagaatatg taaaagcgtt gaatctggta tataaacagc taatgtttga agagttgata
    54421 aaagatgcaa ttaaagaaat ggatcaaggt tatgctgaga gagaagaaag tattcaaaat
    54481 cggattgaaa agttagcata tactgatcca atagatcaaa aaaaacttga taaagcaaaa
    54541 gctgaattat ctaaattgag aaatgaaaaa aataaagaag cgagagaaat attaacgcaa
    54601 gcgatagggt tgaagagtca aacgagaaca cgagatgtag caaaaaaatt agactttgac
    54661 tttggagatt ttgttgattc agcaaatatg aaagctagta aggattttgg caatgatttt
    54721 attggaattg ttactggtca aagaacacct agaatctata gaaataaaag taaatacgga
    54781 ttgttaccgt tcagaggtcg ggatattaag attcacaaag ctgacaagtt ttatatctct
    54841 ttacctaaag gatttagact taaagtaaat ttaggtagca agccaagaaa agcaagagat
    54901 gttgcttcag ttttacaaca cattatggat ggcgattaca aattaaatca aagtaatctt
    54961 atttttgatg gaagaaaatt attttttaat ctaacaattt cttttgaaaa aagctataat
    55021 gaaatggaat tcaagaaagg taatgaattg atcgtaaact ttgcagattt gtgtagcggt
    55081 gaatgtttaa ttaatgataa atctgtaatg tcttttggtg atagccaata tataagaaac
    55141 ttcaaaagta aaattttagc aatgaaatca aaagaacaaa gccgaggagt cttttctaag
    55201 ggcggtcatg gtcgaaaaag aaaattagat caagatcgat gggaagcaat aaagaaaaga
    55261 gaaagtaact ttgttaaaac ttataataat caatgttctg caaaaatcgt taaaactgct
    55321 attcgcaata aagctcaaat tattcgtatc attgaaccag ataaggctta tggcgaatgg
    55381 gcttactatc aattgaaaga acaactagta aacaaaggaa aaagatatgg gatagaagtc
    55441 agaagttagt aatttaaaca atcgttgatt tggcaacgaa atagtgaggg aaaatccact
    55501 cactgaaggg taaactccgt caagttgtgt gaccagacgg aaggacgaat gttgttgagg
    55561 ttttagaact atataaaaca gcatgtttct caaattatga gatgttaaga aaaatgattc
    55621 gagaggtttt agaactatat aaaacagcat gtttctcaaa caaagtgcag aacgaaaatc
    55681 aattcatcac agttttagaa ctatataaaa cagcatggtt ttaaaacaag aaataagtca
    55741 gttttccttt tgctttagta aggaaaaata gagtattctc aataaaactt aaaataagaa
    55801 agaataagca aacctttgtt ttaatagcta atcgaagtaa aacccaaagc catatgaact
    55861 cctgaatcag cgaaagttta tgatgattgg gcttactaat aatttaggaa ttagatggta
    55921 aatctaaatt aaacaatcgt tgatttggca acgaaatagt gagggaaaat ccactcactg
    55981 aagggtaaac tccgtcaagt tgtgtgacca gacggaagga cgaatgttgt tgaggtttag
    56041 aaccacataa aacagcatgt ttctcaaatt gccttagtgg ttgaattgag atctatcacg
    56101 ttttagaacc atataaaaca acatagttct caaattgttt aaaatgattg tcaagttaaa
    56161 aaaatctggg atcatataaa atagtatagt tctcaaatat gattagaaac cctattaaag
    56221 agcaatatta aaccaactta cttaaatcgt ttaattaaga acagtaatct cttttagtca
    56281 gacttttagt ctgacttttt tgtatttcca aaatttattc tttacaaaag cgaacgtacg
    56341 tttgtataat tgcttgtgaa tgggggaata aaaatgtttg attacagtag agaaccggtg
    56401 aatgactatt ttttaatcga catgaagtct ttctatgcta gcgtggaatg tattgaacga
    56461 aatttagacc caatgactac accgttaatt gtcatgagta gatcggataa cactggttca
    56521 ggtctgactc tagcttcttc acctactgca aagaaaaaat atcatatcac taatgttagt
    56581 agagctaatg atttacctta tccattacca aaagaacttt atattgttcc acctcgaatg
    56641 aacttatata taaaaaaaaa tatggaagtt aatcagattt ttagaaagta tgtcgcagat
    56701 gaagatttat tgatttattc gattgatgaa tcaattttaa aagtgacaaa atccttaaat
    56761 ttatttacta gttcagaaac aagaagcgag aggagaagag aattagctaa aataatcaaa
    56821 gatgagattg agaaagaaac aggactaata gcaacagtcg gaataggaga caacccatta
    56881 ttagctaaac tcgcactgga caatgaagcg aagagtagta aagaatttat agctgagtgg
    56941 acttatgaga ctgtacctga aaaaatatgg aaaataaacg agatgactga cttttggggc
    57001 attggatcac gcatgaaaaa aagattgaat catatgggaa ttatgagtat agaagattta
    57061 gcaaattgga atccctacac aattaaaagt cagttaggtg taatcggact tcagttatat
    57121 ttccatgcca atggaattga tcgtacggat attgctattc caccagaacc aaccaaagaa
    57181 aagtcttatg ggaattcaca agttctacca aaagattata cgaggagaga agaagtagaa
    57241 ctagtagtca aggagatggc agagcaagta gccattcgaa ttagacaaca taattgcaaa
    57301 actggttgtg tacgcttagc aattggtacg tcaattttgg aagaaaaatc aggtttctcc
    57361 caccagatga aaataccaat tacagataat acgaaagaat tacaaaatta ttgtcttttt
    57421 ttattcaata agttttatga ggggcaggaa gtaagacata taggagtaac ttatagcaga
    57481 ttagtataca cagattccct acaattagat ttatttagtg atccaagaaa aaagattgct
    57541 gaagaaaatt tggataggat tattgataag attagacaaa aatatggatt tacagctatt
    57601 atacatgctt catctatgtt agaaggtgca agaagtatta cgagaagcac tttaatagga
    57661 ggacacgcag gaggattagg aggtttgcat aatgattgaa tataacgata gaggacgttt
    57721 aaaatggtcg ggattttatt tgtctgatca tactgaaaga atcaatgcag acaatgtaca
    57781 acgtgctaat cataatttgg caaaagaaca aatgacgact gaagagattg cgaaaatact
    57841 gagaactgct gtcataaaaa atagacccgt ctgcattcaa aaagaggaac gaaacgctga
    57901 aggttactat ccagatgatg tagtaggaaa aattattgga tatgacgagc ttggtgtatt
    57961 tgtggaagaa gaaaaagttc actatgatga aattaggaat gtttctttcg cggaattatt
    58021 aaaatggagt cagatatgaa cattattaaa aaatacgaat tcggttatat tacgtatgaa
    58081 cagctaatag aggaattatg gggatatggt caacaattaa ttaatgaagt ggggattgat
    58141 tgcttttgtt tctatattga atccggagca ggataccata agaacagata ctatatattt
    58201 ccatatccac caaaatagtc tcttagagat ttttaaattt tctccaaata ctttcaatta
    58261 ttgtgaaata tttcccttta acagaattag gcagaagtcg cccatcctct ataggt
//
LOCUS       DACDNO010000010        57252 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDNO010000010 DACDNO010000000
VERSION     DACDNO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.010"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            150..635
                     /locus_tag="IWI22_000655"
     CDS             150..635
                     /locus_tag="IWI22_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9168069.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            628..1311
                     /locus_tag="IWI22_000656"
     CDS             628..1311
                     /locus_tag="IWI22_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9168070.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1437..4082
                     /gene="polA"
                     /locus_tag="IWI22_000657"
     CDS             1437..4082
                     /gene="polA"
                     /locus_tag="IWI22_000657"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9168071.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4127..4963
                     /gene="mutM"
                     /locus_tag="IWI22_000658"
     CDS             4127..4963
                     /gene="mutM"
                     /locus_tag="IWI22_000658"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9168072.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            4927..5556
                     /locus_tag="IWI22_000659"
     CDS             4927..5556
                     /locus_tag="IWI22_000659"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9168073.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5608..5940
                     /locus_tag="IWI22_000660"
     CDS             5608..5940
                     /locus_tag="IWI22_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9168074.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6252..6743
                     /gene="nrdR"
                     /locus_tag="IWI22_000661"
     CDS             6252..6743
                     /gene="nrdR"
                     /locus_tag="IWI22_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9168075.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6766..8139
                     /locus_tag="IWI22_000662"
     CDS             6766..8139
                     /locus_tag="IWI22_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9168076.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8139..9065
                     /gene="dnaI"
                     /locus_tag="IWI22_000663"
     CDS             8139..9065
                     /gene="dnaI"
                     /locus_tag="IWI22_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9168077.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9194..9634
                     /locus_tag="IWI22_000664"
     CDS             9194..9634
                     /locus_tag="IWI22_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9168078.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9778..10479
                     /locus_tag="IWI22_000665"
     CDS             9778..10479
                     /locus_tag="IWI22_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9168079.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10476..11597
                     /locus_tag="IWI22_000666"
     CDS             10476..11597
                     /locus_tag="IWI22_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9168080.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11612..12844
                     /gene="pepT"
                     /locus_tag="IWI22_000667"
     CDS             11612..12844
                     /gene="pepT"
                     /locus_tag="IWI22_000667"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9168081.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYILNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPPPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            12976..13884
                     /locus_tag="IWI22_000668"
     CDS             12976..13884
                     /locus_tag="IWI22_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9168082.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            13919..14191
                     /locus_tag="IWI22_000669"
     CDS             13919..14191
                     /locus_tag="IWI22_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9168083.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14202..15248
                     /locus_tag="IWI22_000670"
     CDS             14202..15248
                     /locus_tag="IWI22_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9168084.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            15499..18108
                     /gene="clpB"
                     /locus_tag="IWI22_000671"
     CDS             15499..18108
                     /gene="clpB"
                     /locus_tag="IWI22_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9168085.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(18259..18930)
                     /locus_tag="IWI22_000672"
     CDS             complement(18259..18930)
                     /locus_tag="IWI22_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168086.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(18923..19417)
                     /locus_tag="IWI22_000673"
     CDS             complement(18923..19417)
                     /locus_tag="IWI22_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9168087.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(19437..20657)
                     /locus_tag="IWI22_000674"
     CDS             complement(19437..20657)
                     /locus_tag="IWI22_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9168088.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            20804..22639
                     /gene="lepA"
                     /locus_tag="IWI22_000675"
     CDS             20804..22639
                     /gene="lepA"
                     /locus_tag="IWI22_000675"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9168089.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            22835..23746
                     /locus_tag="IWI22_000676"
     CDS             22835..23746
                     /locus_tag="IWI22_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9168090.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            24742..24993
                     /locus_tag="IWI22_000677"
     CDS             24742..24993
                     /locus_tag="IWI22_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168091.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            25076..25348
                     /locus_tag="IWI22_000678"
     CDS             25076..25348
                     /locus_tag="IWI22_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168092.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            25438..25677
                     /locus_tag="IWI22_000679"
     CDS             25438..25677
                     /locus_tag="IWI22_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168093.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            25824..30107
                     /locus_tag="IWI22_000680"
     CDS             25824..30107
                     /locus_tag="IWI22_000680"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP9168094.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVKVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            30206..30571
                     /locus_tag="IWI22_000681"
     CDS             30206..30571
                     /locus_tag="IWI22_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168095.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            30573..31025
                     /locus_tag="IWI22_000682"
     CDS             30573..31025
                     /locus_tag="IWI22_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168096.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            31639..32949
                     /locus_tag="IWI22_000683"
     CDS             31639..32949
                     /locus_tag="IWI22_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP9168097.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLIKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            32972..33283
                     /locus_tag="IWI22_000684"
     CDS             32972..33283
                     /locus_tag="IWI22_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168098.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            33644..34900
                     /locus_tag="IWI22_000685"
     CDS             33644..34900
                     /locus_tag="IWI22_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9168099.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            34897..35442
                     /locus_tag="IWI22_000686"
     CDS             34897..35442
                     /locus_tag="IWI22_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168100.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            35443..35943
                     /locus_tag="IWI22_000687"
     CDS             35443..35943
                     /locus_tag="IWI22_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168101.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            36024..36580
                     /locus_tag="IWI22_000688"
                     /pseudo
     CDS             36024..36580
                     /locus_tag="IWI22_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36600..36944
                     /locus_tag="IWI22_000689"
     CDS             36600..36944
                     /locus_tag="IWI22_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168102.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            36963..37874
                     /locus_tag="IWI22_000690"
     CDS             36963..37874
                     /locus_tag="IWI22_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9168103.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            37953..38945
                     /locus_tag="IWI22_000691"
     CDS             37953..38945
                     /locus_tag="IWI22_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9168104.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            38942..39160
                     /locus_tag="IWI22_000692"
     CDS             38942..39160
                     /locus_tag="IWI22_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168105.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            39173..39583
                     /locus_tag="IWI22_000693"
     CDS             39173..39583
                     /locus_tag="IWI22_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168106.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            39635..42163
                     /locus_tag="IWI22_000694"
     CDS             39635..42163
                     /locus_tag="IWI22_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9168107.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            42179..44389
                     /locus_tag="IWI22_000695"
     CDS             42179..44389
                     /locus_tag="IWI22_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168108.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            44397..45488
                     /locus_tag="IWI22_000696"
     CDS             44397..45488
                     /locus_tag="IWI22_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9168109.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLRSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            45509..46051
                     /locus_tag="IWI22_000697"
     CDS             45509..46051
                     /locus_tag="IWI22_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168110.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            46273..46503
                     /locus_tag="IWI22_000698"
     CDS             46273..46503
                     /locus_tag="IWI22_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168111.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            46517..46849
                     /locus_tag="IWI22_000699"
     CDS             46517..46849
                     /locus_tag="IWI22_000699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168112.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            46894..47442
                     /locus_tag="IWI22_000700"
     CDS             46894..47442
                     /locus_tag="IWI22_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9168113.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            47449..47901
                     /locus_tag="IWI22_000701"
     CDS             47449..47901
                     /locus_tag="IWI22_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168114.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            47998..48333
                     /locus_tag="IWI22_000702"
                     /pseudo
     CDS             47998..48333
                     /locus_tag="IWI22_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(49058..49729)
                     /locus_tag="IWI22_000703"
     CDS             complement(49058..49729)
                     /locus_tag="IWI22_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9168115.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            50033..51664
                     /locus_tag="IWI22_000704"
     CDS             50033..51664
                     /locus_tag="IWI22_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9168116.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            51667..52746
                     /gene="uxuA"
                     /locus_tag="IWI22_000705"
     CDS             51667..52746
                     /gene="uxuA"
                     /locus_tag="IWI22_000705"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9168117.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            52820..54247
                     /gene="uxaC"
                     /locus_tag="IWI22_000706"
     CDS             52820..54247
                     /gene="uxaC"
                     /locus_tag="IWI22_000706"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9168118.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            54331..54978
                     /gene="eda"
                     /locus_tag="IWI22_000707"
     CDS             54331..54978
                     /gene="eda"
                     /locus_tag="IWI22_000707"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9168119.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            55098..56474
                     /locus_tag="IWI22_000708"
     CDS             55098..56474
                     /locus_tag="IWI22_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9168120.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            56484..>57252
                     /locus_tag="IWI22_000709"
     CDS             56484..>57252
                     /locus_tag="IWI22_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9168121.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDT"
BASE COUNT        19560 a         8906 c        12077 g        16709 t
ORIGIN      
        1 ttggctgttc ttattatttg atgcaggaca gctttttcac gacctctgta aactatagga
       61 atctgttaaa tcaagggagc agaacttgcc ttttacgcta cttttggtac aataatgtat
      121 gattaaaaat aaactggagg gtttctatct tgaacatagg taagccgaga aaactaggaa
      181 aaacaataaa tgaaatcgag gaaggcgact cgttatcctt aacagaatct attgaagata
      241 accagctttt gctgtatttg ggattgacga atgatgcaaa tcctttgtat atccaacatg
      301 actttgcaca aaaaacagaa tatcaacagc caatcgttcc atcagtgatg ctgatgggga
      361 tcatcacaag tgcaatttca aagcatcttc ctggaccagg atcacatgtt gtcaattttt
      421 ctgtcaattt tattgaaccg gttttccatt atgaaacact gacttttcag tttgaggtca
      481 taaaagtaga taagatgaag gatgtggtga cgatttctgt agaagcggag aatacgaacg
      541 aagatcgtgt gttggatgct gtcgttatgg tccaaccgcc acaagttcct gaattggaaa
      601 tagcaatcga aagtgaaggt gaattgaatg aataatccaa gtacatcaat gtcaggtatc
      661 aatcgttttt atgcaaaagt ttatggattt ttaggtatgg gacttgccct tagtgcagtc
      721 acttcttact tggctctttg ggtatttcct ttgcaattag caacttttat caataatttt
      781 ccactaggct ttgtgggtct gtggatcgtt gaactgattt tagtaatggt tctaggcgta
      841 aaagcacaaa agaatccgag cttagcaatc ggtggattta ttgcctactc tcttttgaat
      901 ggcataacat tagcggttac tcttgcatac tacgatattg gaacagtaac gcaagctttc
      961 ttgacagcta ctttgatgtt catggggatg tctgttgtcg gagtaacaac aaaaagagat
     1021 cttagcgggg ttggaagagc aggacaggca gctttaatcg gagtgatcat tgcactagtc
     1081 ttgaacatct tcattttgag aagtagcgta gttgagctgt tcatctccct actgttagtc
     1141 gtcatctttg cagggattac agcttatgat aaccaaagca tccgtaatat ctatgtccag
     1201 acaaacggaa cagcacatag tggaattgct gtattcatgg cattgaagct gtatttggac
     1261 tttatcaact tgttcttggc gtttctgaga atctttggac ggaacaacta aaaaagaaaa
     1321 aaattcatca gttggggcta taccgaatta tttggtatgg tcccgctttt tcttttcgca
     1381 ttccgcagga ttctttgtta gaatgaataa gaactttaca gtaaggagtt aagaagatga
     1441 caaaaaataa attattactc gttgatggga acagtgtggc ttttcgcgca ttctttgcgc
     1501 ttcacaattc tcttgaacgt ttcaaaaata gaaacggtct tcatacaaat gcaatctatg
     1561 cattcaatac gatgtttgaa aatgttatgc aaaaagagca accgactcat gttctggttg
     1621 cgttcgatgc aggaaaaacg acttttcgga cagaaatgta tgcggagtac aaaggtggaa
     1681 gatccaaaac acctggcgaa ttcaaagaac aaatgccata tatacgtgat ttgttgacag
     1741 gactaggggt acagtattac gaattgccca attatgaagc agatgatatt atcggtactt
     1801 tggcagaaaa agtagacaaa gatcaatttg atgtcgtcgt gctttcaggt gatcgggatt
     1861 tgactcaatt agctagtaaa gaagtaaaag tggatatcac tgtaaaaggt gtcagcgata
     1921 tcgaaagcta tacaccagaa catgtagcgg aaaaatatga tgggttgacg ccaaaacaga
     1981 ttattgacat gaagggacta gccggagatg cctctgacaa tatccccggt gtgactaaaa
     2041 tcggtgaaaa aacagctatc aagctattga aacaatatgg ctcagtcgaa ggaatttatg
     2101 aacatatcga tgaaatgaag caaagcaaaa tgaaagaaaa tctgataaat gacaaagagc
     2161 aggctttctt atcgaaaaaa ttggcgacca tcaatacttc agcgcctgtg gatgtctcta
     2221 tcgactcatt gaagtatgaa gggaaaaatc tcgataaact cgttccgttt tataaagaaa
     2281 tggatttcaa tcagttcctt tctaaattga atatcgtaga agaagaagta aaaatggatg
     2341 atattttgtt tgaagtcgtt catgaattca aagaggaaat gtttactacg gatatggcgt
     2401 tgtacgtgga aatgatgggc gacaattatc atacagagga aatcgtcgga gtagcgtggg
     2461 ggacagaaaa gaaaatttat gtcacgaacg atctatctgt attcgaaaac cgagcgttcc
     2521 attcttggat cactgatccg actcggctga aaaaagtata tgatgcgaaa cgaacttatg
     2581 ttgctttaaa ccgctatact ggaaaaacga aaggaatcga ttttgatgtc ttgctaggtg
     2641 cataccttct cgatacgaat gaaaaaagca cagatatcga aggaatagct gctcattacg
     2701 gatacaacga tatccaatca gatgaagctg tatacggcaa aggggcgaaa aaaggtttac
     2761 cagaagaaga ggaactcttt tttgctcatt tagcgcgaaa agtagctgca atcaatgcgt
     2821 tgacaccaaa attgagccaa gaactggcag aaaaaaacca agaagatctt ttccggaaaa
     2881 tggaactgcc attgtctatc attttggcag agatggaaat aagcgggatc aaagtagatg
     2941 cgactcgctt acaagaaatg aagggcgagt tctctgctcg cttaagagag atcgaacaaa
     3001 aaatctacga agaagccggc gaagaattca atttgaattc accaaagcaa ttaggagtga
     3061 tcttatttga aaaaatggga ttacccgtta tcaaaaaaac aaaaactgga tattccacag
     3121 ccgtagatgt actggaacag cttcgtgaac aagcaccaat cgtagaggat atcctcacct
     3181 atcgtcaaat tgcaaaaatt caatctacct atgtcgaggg actgctgaaa gtgatccaat
     3241 cagatggaaa agtgcatacc cgttatgtcc agacattgac ccaaacagga cgattaagct
     3301 ctgttgatcc taatttgcag aacatcccga ttcgattaga tgaagggaga aagatacgtc
     3361 aggcatttgt tcctagagaa aaagactggc tgatttattc ttctgactat tcacaaatcg
     3421 agttacgcgt acttgcgcat atctcaaatg atgaacattt gaaagctgcc ttcctagaag
     3481 gtcaagatat ccatgccagc acagcgatgc gtgtatttgg aatcgaaaag gcagaggatg
     3541 tcacaccaaa tatgcgtagg caggcaaaag cagtgaattt tggtatcgtg tatggcatct
     3601 ctgattacgg actttcacaa aatctaggaa tcagccgaaa agcagcacag caatacatcg
     3661 atacgtactt tgaaaaatac ccaggtgtca aagaatatat ggaaagaatc gtacgcgaag
     3721 caaaagacca aggctacgtc gaaacgctgt atcaccgccg tcgatatctg ccagacatca
     3781 attcaagaaa ttacaatatc cgttcttttg cggaacgaac agctatcaat acaccaatcc
     3841 aaggaagtgc tgctgatatt ttgaaaatcg cgatgatcga attggataaa cgattaaaag
     3901 aaacaggtct tcaagctacg atgctgttac aagttcatga tgaacttgtc ttcgaggtac
     3961 cggaaaaaga acttgaatca ttagataaat tggtgaagga agttatggaa caagctgttt
     4021 ctcttcatgt accactgatc actgacagca gttggggaaa aacgtggtac gaagcaaaat
     4081 aaacattgtt taagacagag caatataatg aaggtgaaaa gaaaaaatgc cagaattacc
     4141 agaagtagaa accgttcgta aaggattgga acggttagta gtaggaaaaa cgattcaaaa
     4201 agttcaggtc ttgtggccca agatcatcga gcaaccagag acacccatct ttgaggcttc
     4261 tcttgttggc gaaaccatcc aatccattgg acgtagaggg aagtttttaa tttttcatct
     4321 cgatcattgt gaactgatct cacacttgcg aatggaagga aaatatcaat ttacgaaaga
     4381 aaatacacca atcgataaac atacgcatgt acttttcttt tttgaggatg gaagccaact
     4441 tcgctacaac gatgtacgaa aatttggtcg tatgacaatc gtcgaaaaag gagcaagtgc
     4501 aacttaccga ggaatcatga aattgggacc tgagcctttg ccagatagct tcttattagc
     4561 agatttcgca aatggactga aaaagtccca caaagcaatc aagccattgc tgctagacca
     4621 acgtctagtg actggtctag gtaatattta tgtagatgaa gcattatggg aagcgaagat
     4681 ccatccagaa caaccggcga acacactgcg ccccaaagag gtagaacaat tacgtctatc
     4741 gatcattgat gttttggatc gtgcaataga agccggaggt accacgattc gcagttattt
     4801 gaatgcatta ggagaatcag gcggatttca agtagcactg catgtctatc agcaaactgg
     4861 caaaccatgt atacgttgtg gaacgccgat cgtgaaaaca aaagtggcac aaagggggac
     4921 gcactattgt ccgaattgtc aaaggctaaa aatcgcaaag taggatttgt tttaggtttg
     4981 acgggtggaa tagctaccgg taaaagtacg gcagccaaag tctttcagtc tcatggattt
     5041 cctttgatcg atggcgatgt gattgccaga gaaactgttg agcctggaac gcttgctctg
     5101 aggaaaattg tatcagtgtt tggccaagag gtattgcagc ctgatgggca actagatcga
     5161 gggaaacttg ggatgattgt ttttccatca aaggaaaaac ggcaaaaact tgatcagcta
     5221 ttagatccgt ttatccgaaa agcgataaaa gatcaaatcg cctctctaag ttccaatcat
     5281 cctttagtaa ttgtagatat cccattgctc tacgaaggtc attatgacca ctatatggat
     5341 gcagtagctg tcgtttatac tacgccagaa actcagcttc agcgtttgat gaaacgtaat
     5401 caactgacgc tagaacaagc gcagcagaga attagtagtc aacttccaat cgaagaaaaa
     5461 aagcagcgtg cggatatttt gttcgacaac aatggaacaa aagaaaaact tgtgggacaa
     5521 atcgaaaatt ggctttttgc taatcgcttt ctttgagtat ctgtgtcaat tttgctatcc
     5581 tgagtatata gaaagtgagg gatagcgatg ggatcattaa aaaatcaaaa ggataaagtc
     5641 gtaggtaacg caaaagaaaa aatcggcgag atgctcaata aagaaaatct aatcgatgaa
     5701 ggtcgtacgc aatcagaaac agcagaagaa aaagaaaagc agtaccaagc aagaatcgat
     5761 gaaatagaag accagcaggc agaagaagaa gcgaagcggc aagatttgac tcaagaacga
     5821 aaaaatccga cacctgatcg tagtaaaaca ccgctcccag aaagtgacca cattcccgat
     5881 gagcaaaaaa tcgaggaaca gcgaatgaaa cactatacag gaatcgaaga gaagttataa
     5941 aatagtaaaa aatcgagatg tgacttgaag cagatgagaa ggtgttcatc tgcttcaagt
     6001 tttttgtgct agatgactaa atcagctttt gtgtaagtta tttctcagta atcaaggtga
     6061 tcagtattgg aaaattgaaa tttagtaagg acaggggaca caattacgca tgaaagtaga
     6121 aaaaattgtt cttatatccg tgttttttgg gtaggacagt cgcgtttgtg atttgtttcg
     6181 tgttataata caactagttg taaagaaaga cacatccttt atataaaatg cgatgcaaga
     6241 ggtgagacaa aatgagatgt cccagatgtc aacataataa ttcacgagta atcgacagcc
     6301 gtcaagcaga tgatggacgt gcgatccgtc gccgtcgcga atgtgagaat tgttcgttcc
     6361 gattcactac atttgaacga attgaagctg ctccgttatt agtaatcaag aaaaacgggg
     6421 aacgcgaaga gttcaatcgc gataaaattt taagaggact catccgttca gcagaaaaaa
     6481 gacctgtagc tatggaacaa atggaacaaa tcgtcaacca tgtcgaaaac cgcgtacgca
     6541 gtctcggaga aaacgaagta tcgacgaatc taattggtga atatgtgatg gaagatttag
     6601 ttgatttaga cgaaatcgct tatattcgtt ttgcaagtgt ttatcgccag ttcaaagata
     6661 tgtctgtttt cttaaaagag ttgcaagata tcgtggataa agcaaaagaa aataataaag
     6721 agaataataa agaaaacaat taagaaacaa aaaggaggca tttccttgga aaatgcttgg
     6781 aaagagcttc aaccaaaaaa tatttaccaa ggaagaaaag cggctccgct gagagaagac
     6841 agcaagaacg ccttgctgta tctttatcag ccactaatcg gcggagaagc cttgagtatc
     6901 tatttcaccc tgttatcaga aatctctcaa gaatctggca aaggtcctga agggatgcat
     6961 gcagatctgc ttagtagtct aggctgcggt atcccgcaat tttatgatgc aagaaaaaag
     7021 ctagaaggaa tcggcttact ggatgtttat tttaaagaag atagtgatct gggacctcgt
     7081 tttatttatg agctgttaga accactcatt cctaaagcat ttttcaaaga ctctgttctg
     7141 gcttttcttc ttctggaaaa agttggtgaa cgacgattcc agaagcttac ccaattcttt
     7201 gaacctaaaa ggcatacagc agagggatat caaaaaatca caaaaaaatt tttagatgtt
     7261 tatcgcttca atgaagcagt gtacgctgct aatcaagaaa acatcgaatc gctgaataaa
     7321 tcttttgaag aatctgaatc agtagcatta cttgaagcag attcgacatt cgattggcag
     7381 tttttgaatg attggctggc aaaaaaacat attgcaagtc ttgatgatcc aatcatcaaa
     7441 caagtcgaca tgtatcatca gctttacggt ttcgatgaat tgacacttgg cgaactgatc
     7501 agtcaatctt atgactttag tgaacaaaaa gtatctgaaa aagaactgca aaaagccatt
     7561 ttggcaatgc agagacgtcc ggtgaagcaa acaaaagaaa cagttgcggt ttctgaaaac
     7621 agtaatgaac cctcgtcaaa cgaacatttg acaaacgcag cgcttcagct gataaaagaa
     7681 gcacagagca ttccaccgat gaaatatctg gaagcaatca agtcagaaaa aggcggatat
     7741 gtttctaaac aagaaaactg gttactgcaa gatcttgttt ctcaatcagg tctttccagt
     7801 agcgtgatca atgttttgct gaattatgta ctagtgatca agaataatgc tagcttaaat
     7861 gccagttatg taaatacagt ggcaaatgaa tgggcacaaa agaaaatcgc cacagcagaa
     7921 gaagcgatcc agcacattcg gcaaataagt aatcaagcaa aacaatcaaa acaaaagaag
     7981 caaacaaatt atcaaactgg caagcgaaat gttcgacggg aaaagcttcc tgattgggtc
     8041 aaccagccaa aagatgaaaa acagatcagc agcgagaaaa aagcagagat tgaccgccgt
     8101 tttaaagaat acctggcaaa aaaggagggt gatatctagt ggaagacgta ggcaaagaat
     8161 tgaagaggat catcagcaaa cgcaattatc aagaaaagta tgctgaaatg atcgctgaag
     8221 tcttaaatga tgcggatgtt caagcttttc taagcgcaca ccaagaagaa ttgacacaag
     8281 cggatattga aaaaagttac gctaaattgt atgaattcgt gcaagaaaag cgtaaatatc
     8341 aaaaaaacga tccaacgatg atcgctcccg gctatgagcc gaagctgacg ttgaattttc
     8401 attatgtgga tgtcacttat gtaccaacgg aagcattgtt agcacggcaa agacaagaag
     8461 aaatcagaag ccgagtaaaa gcaatggata tgccaaaaga tattcaatcg gcttcttttg
     8521 aaaactttga acgtacagat ggacgaggat atgcgagctt agaagcccta gattttgtag
     8581 agaaatatac tgctgatcca aaagggtatc ataaaggact gtatctagta ggaagttttg
     8641 gtattggaaa aacatattta ttaggagcaa cagcgagaga attagcgatt gctggattca
     8701 ccacgacgct agtccatttt ccgacatttg ccgttgagat gaaacaggca atcggaaaag
     8761 atcaagtggc agaaaagtta gatgcagtga aacgttcacc tattttgatg atcgatgaca
     8821 ttggtgcaga tgcgatgagt agttggatac gagacgaagt attcggtgtt atccttcaat
     8881 atcggatgca ggaacagtta ccaaccttct tctcatcgaa ttttacaatg gatgaattgg
     8941 aacagcattt gtccgtcact caacgtggag atgaagagcc gctaaaagcg aaacggataa
     9001 tggaacgcat acgttatttg acaaaagaaa tcgaaatgac tggtcgcaac cgacgcaatg
     9061 gttgaacagt tgacaagtcc aactgacatt tgacctagta gtaaaagcag gtcagcgaat
     9121 cattatatgg acagaaatga ccggagacaa tcaccgcaac aagtaatgca gaaacttaaa
     9181 cggaggattt tttatgaggg ctattttttc tagtatatct gtagttggtc tattttttct
     9241 tttagcaggc tgcggtagcg cagatagcaa cacaggttct gttgattcga ttgatgcggg
     9301 tcaaacagta aaaacaagca gtacgaaaac ggagagacaa agcatatttg tcggaacgat
     9361 tgaaagtatc actcaagtag aagcagaaga agaaacgctt cagttgaaac tcgtcgatgt
     9421 catggccaaa gaagatccag cgaatatggg aacgtcattc agcaatgacg gagtgatctt
     9481 aaatgctact gctgaaaaaa taagcgtgga cataaatgaa ttgaaaatcg gtgacaaagt
     9541 ccaatttaca ttagttgaga tgccgatcat gacgatgtct atcccaccgc aagttcctgg
     9601 agattcgatt attgatgttt cattgaacaa ataaccaata acattttttc aaaaataaga
     9661 aaatgttgtt ttatagaacg aaaaggtata ttttttaagg agatatggga atttttttat
     9721 tcctagtctc cttattttat ttggaaaatg ctataattat ctagaaggag tgaagctatg
     9781 aaccatttag atttatctca gcgcttagcc gcagtgggtg actttgtccc tgaatccgct
     9841 cgtttagcag acattggatc agatcacgct tacttacccg ttgcattgat attaagagga
     9901 aaaatcgaat atgcagttgc tggtgaagtc gttcaaggtc cttatgaatc agcacgtaag
     9961 caagtcaaaa aagatggatt agaagaccgt atcaccgtac gccttgcaaa tggtctagat
    10021 gctatccagc cacaagatgg tattacagct gtcacgattg cgggtatggg aggttcactg
    10081 atccgagata tcctagaagc tggcaagttg aatcaacgct taagcggaca agaacgtttg
    10141 atccttcagc caaacatcgg agagcgtaca ttacgatctt ggctgcaaga aaatcagtat
    10201 aaaatccaat cagaaaaaat acttgaagaa aataaaaaaa tatatgaaat cattgtagcc
    10261 gaaaaagaac aaaaaccagt tacttacaat gaaatggagc tgatgttcgg gccatttctg
    10321 cttgaagaga aaagtgagat cttcaaatca aaatggacaa gagagcttca acaaagaaaa
    10381 gctatccgtg aacagctcaa acaagcagct aatcaagagg cacgtcaaaa acaaatcgag
    10441 caggaaatcc aaatgattga ggaggtttta aagccatgac aatcagcgga cgcactttca
    10501 tccaaaagtt tgaaagctat tgtcctcagt ggctagctga agaaggagac ccagtcggac
    10561 tgcatatcgg aacattggat aagccaatcc aacgagtaat gatgacatta gacgttcgtc
    10621 cagaagtggt cgaagaagca atcaagaaaa aaatcgattt gctgattgct aaacatcctc
    10681 caatctttcg tcctgtaaaa cggctggtta ctgatcagcc acaagaaaaa atgtatgcag
    10741 atttgttgaa acatgatatt gccgtctatg cagcacacac gaacatggat attatttggg
    10801 atggactgaa tgactggttt tgtgaacttc tcggtattga agtggagagt tatttagtaa
    10861 agactcatga gattcattat aaaaaactgg ccgtttatgt acctgtggat catgcacaga
    10921 aaatgcgtga agtattagct gcagctggtg ctggtacaca aggtgattat accggtacaa
    10981 gttttacctc gattggacac ggtcgattta ctccggaagc tggtgcacaa ccagcgattg
    11041 ggaaagtagg gaagacagaa caagttcaag aggcaaaagt tgaggttatt ttgccggaaa
    11101 ctatcgaaaa acaagtgatt caagccatgc ggtcagctca tccttatgag gaacctgctt
    11161 atgatctttt cgctatagat gaaccagtcg aaatgtttgg gttaggacga gtaggtgaat
    11221 tgccgcaaga aatttctata gaggcatttg tagaacaagt aaaagaagcg ttccaactag
    11281 atggactgag aatagtccag ccaaaaaatg caaaatcctc tgtgaagcgc attgcgattt
    11341 gcggtggaag cggtgaaaaa ttttatccgc aagcaatcgc ccaaagagca gatgtctata
    11401 tcacgggaga tatctattat catacggctc atgatatgca aagcgctggg ttgattgcaa
    11461 ttgatcccgg tcattatatt gaatcgttat gcaagcaacg atttatcgag aaatttgaat
    11521 cttggaaaca agaagaaaat tgggatatta actttttcgt ttctgaaaca aatacaaacc
    11581 catttcaatt taattaaaag gagagaatac tatgtacgaa aatcttttgc cacgtttttt
    11641 acgatatgtc aaaacagaaa cacgctccga tgcaacaagc aaaacgactc catcgactca
    11701 gacacaagtt gcttttgcgc aagaattgaa aaaagaatta gaagagttag gcttagctga
    11761 tgttttctat aatgaagata acggattcgt tatgggaaca ttaccaagca atgttgatca
    11821 tgaagtgcgt tccattggat ttatttccca catggataca gcagatttta atgcagtagg
    11881 agtcaatcca caaatcattg aaaactatga tggcgagtct acgatcgtac tagatgcgga
    11941 agaacgctat atattgaata caaaagattt tccaaatttg aaaaattatg aaggccacac
    12001 attgatcaca acagatggca gtacattact gggagcagat gacaaatcag ggattgcgga
    12061 aatcatgact gcaatggaga tcttgatcaa taatccttct atccctcacg gtaccatcaa
    12121 agtggctttt ggtcctgatg aagaaatcgg tgtcggagcg gataaattcg atgttgagca
    12181 attcgatgta gattttgctt atacagtcga tggtggtcct ttaggtgagt tgcagtatga
    12241 aacgtttagt gctgctcaag caaatattac gatccaaggg aaaaatgtac atccagggac
    12301 ggcaaaagat acaatgatca atgctctaca gttagcaatc gattttcata atcagcttcc
    12361 agccgatgaa gttccagaaa aaacggaagg atacgaaggt ttcttccatt tgatggcatt
    12421 ggatgggaat cctgaagaag cttctatgag ttatatcatt cgcgatcatg atcgtgaaaa
    12481 attcgaagca cgcaaagcac aaatcacaca tatccaagaa gagttgaatc agcgatttga
    12541 tcaagaacga atcaaagtag atatgtatga tcaatattac aacatgaaag aaatcattga
    12601 aaaagatatg agcatcgtgg aattagcgaa acaagcaatg atcgagcttg gtatctctcc
    12661 agtgatcgaa ccagttcgtg gaggaacaga tggttcaaaa atctcttaca tgggtattcc
    12721 tccgccaaat ctatttgccg gaggagaaaa tatgcacgga cgattcgaat ttgtttcttt
    12781 acaagtaatg gaaaaagcaa cagacgtgat tgtaaaaatt gcagaattga acacaaagag
    12841 ttaaaatcga aaaagaacag cttatgacaa ggcgttcgtc ccaatgatgg aatgtcatct
    12901 gtctaaggct aaagggcagg acaatggaat tggttgtccc gccctctttt ttgaagagta
    12961 aataggaggt aaagaatgga aaagctgcta gttttttata acaaaagttc agggaaagat
    13021 gagggtgaga aattagcacg ctggtttcaa gattatgtaa aagaaaagca gccagaatta
    13081 tatgtagaac tagctgaaac tggcccatcg attgatgatg cggaattagt caagaaagca
    13141 gaagccatag aagctgatac tttagtaatt ataggtggag acggcacgat tcatcatatc
    13201 gtccaagcgt tccaaaaaac attatctcaa taccatgtcg gattacttcc aggtggaact
    13261 gtcaacaacc ttgcacgtgt tcttgagatt cccctcaaaa aagaagaagc tgctgacgtg
    13321 atcctggaag gcaatgtacg gaaaatagac tatggtcaag tcaatgatga agtcattatc
    13381 tctacactga caatcggaat cttagcagat acagcagttt ggatcagtca agaagaaaaa
    13441 cagcgttacg gctcgtggat ctttatcaag aagtttttta agttattgct gaagaagaaa
    13501 aaatatcatt tggatttaga aacagacgag gagcattgga aaggaaaatc ccaactgttg
    13561 acagtcacga tgtctaactc cgtgggcggt tttacaaatt ttgacgactc agcgacccta
    13621 gatgatggaa aatttcatgt aacgatcgta ccttccttag atattttccg atttattttt
    13681 tatttgccta gaatcatgag aggcaaattc tatacgatcc ccggcattac ttatttcaca
    13741 gccaaacgca tgaaaatcca agcgcaggaa aaatctgtgg gcactcgaac ggatggagac
    13801 acgacagatg atcttccagt tgaattatct gttgtatcaa aaggattaac agtttattca
    13861 ccaaagaaag aagaaaaagc ttagaaatag tagttaacag aaagttggaa gaagagtaat
    13921 ggatgtattg ttacacaatc ctttgtggtt ttaccttttc attgtgaatt tttacttatt
    13981 ttgtctgatg gggtatgata aacatcaggc tagaactagc ggctggcgtg ttcctgagaa
    14041 taatttgata tttgtcagcc tgttaggcgg tggtttggga ggattgatct ctcgaaaagt
    14101 tttccatcat aaaactcgga aaaagaagtt tactatagga ttcgctgcag gtgtacttgt
    14161 tgatttgatt ctcatttatt ttttccatta aggagcagga gatgtcgtct aaaggaacga
    14221 aaactaaaat cattctgaat ctgcttttgc tgattgtgat ttttgccatt atttattatt
    14281 taatcaatca atcatttgca gatatctttg ccgaattgct gtctaccagt gttgttgttt
    14341 tggcagcggc tgttatatta gggacagttt accaaatcgc tgaagggcgc tcaatcaaag
    14401 aaattgctga acctttcgcg ccgaatttta caacgaaaga tgggttcttg acctctagct
    14461 atattgcgtt ttaccgaatc gtttcgtttg gtacaggaac gctgatctcg gagatttatt
    14521 tttataacaa aaaaggaatg aagctatccc agggagcagg ggtgacagca ctccatatga
    14581 ttatgtacaa actggcagtg atcacttatg cattagctgg cttggtcatc caattctctc
    14641 ttttttattc taatggtcca tcaatgatct ggtttatcat cgctggtatc cttttgacga
    14701 tcgtgattgt tgccttttta ttggcgatgt ccgtcagctt gaatgttcaa atattttttg
    14761 tcagcttatc gaataagtta tttaaaagtg agcgtttgcg gaaatgggtg gatacatgca
    14821 atcagcagat ctactcttta agggaaacgg tccaaaccat tatccaagac aggactgcgc
    14881 tattgcggat ttatctatgg aatatgttaa aacttgtatt ttggtatgta cttccttatt
    14941 tgattctgat tgaaaaccat cctaaaatag attttctact gaccatttcg tttatcagct
    15001 ttgcagtagt gctttcaggg gttattccta cgcctgctgg tattggttcc tttgaatttg
    15061 tctatttgtt attattcaga ccagttgttg gaacagttga cgcagtttct tcgcttttac
    15121 tgtatcgttt cagttccttt atcttgccat ttctctatgg atttgtgtat gtgattgctg
    15181 aacggcgaag agtgatcaaa gaagaaataa tagaagtaaa aaaagagaat aaagaaaatc
    15241 aagggtaggt cttttataag atctaccctt tttatcaaat caaagaaaaa cgttatggat
    15301 aagataattt tacgtttgtt tggctctttt tttatataca tacaatcaat tttgtttttt
    15361 taagatttat ataagtcgag aaaaaaagtc aaaaaactat gtgtatattt tgaccaaaat
    15421 tgaccaaagc gatataatag tttttgtaaa gatcaaagaa ataagtgatc ttacctttaa
    15481 ttaggagggt atacctgtat gaatattgag aaaatgacta cgacgcttca agaagcgatt
    15541 gctgaagctc aacagattgc ggtcactcga aaacatcaag atatcgacat tgcccatgta
    15601 tggaaaatct ttttacaacc taatcatttt ggtcgaaatt tctatacgga tgccggattg
    15661 gatgtcgaat cttttgaaca tgaaattgat cgtctactgg atgagtatcc agttgtttct
    15721 ggtggaaacg tccaatatgg acaaaacctc agccagaatt tctttcgatt attgaatgaa
    15781 gcagaccaaa tacgtgaaag cttcggcgac gagtttcttt caacggaagt cgttatttta
    15841 gcgttgatga aattaaaaaa ttatccgtta actgtttatt tgaataaaaa cggattatct
    15901 gaaaaagaat tgcgtaaaaa catcgaagag atgagaggag gagaaagagt gacctcacaa
    15961 aaccaagaag aacaatacaa agcattagaa aaatacggcg ttgatttagt acagcaagtc
    16021 aaaagcggaa aaatggatcc aatcatcggt cgtgatgaag aaatccgtga tgtcatccgt
    16081 attctttcac gaaaaacaaa aaataatcct gttctgatag gagaacccgg tgtagggaaa
    16141 acggcaatcg ttgaagggct tgctcaacgt atcgtacgca aagatgtgcc tgaaaattta
    16201 aaagataaaa cgattttttc attagatatg ggtgcattga tcgctggagc aaaattccgt
    16261 ggtgaatttg aagaaagact aaaagcagtc ttgaaagaag tcaaaaaaag tgatggacgt
    16321 atcctcttgt tcatcgatga aatccacaat atcgtagggg caggaaaaac agaaggaagt
    16381 atggatgccg gaaatctgtt gaaaccaatg cttgctcgcg gtgaattaca tttgatcggt
    16441 gccacaactt tagatgagta ccgtcaatat atggaaaaag acaaagcgtt ggaacgtcgc
    16501 tttcaaaagg tattagtcaa agaaccaaca gtggaagata cgatcagtat tctgcgtgga
    16561 ttaaaagaac gttttgagat ccaccatggt gttaacatcc atgacaacgc attagtagca
    16621 gcagctactt tatctgaccg ttatattact gatcggttct taccggacaa agcaatcgat
    16681 ttgatcgatg aagccagtgc aacgattcgg gtagaaatga actctatgcc aacggaatta
    16741 gatcaagtta ctagacgttt gatgcagttg gaaatcgaag aagctgcact gaaaaaagaa
    16801 tcagatgatg caagtaaaaa acgcttgaaa aatttgcaag aagaattagc tgaacttcgt
    16861 gaagaagcaa atgcgatgaa gatgcagtgg gaaacagaaa aagaagaagt caattctgtt
    16921 tcagcaaaac gtgctgaaat cgataaagca aaacacgaat tagaagatgc agaaaataac
    16981 tatgatttag aacgcgctgc tgtattgcgt catggtacga tacctcaatt agaaaaagaa
    17041 ttgaaggaat tagaagctaa agcaaaagac agcgagatta agatggttca agagtccgtt
    17101 accgaaaatg agattgctca agtcgttggc agattaacgg gtatccccgt aacgaaactt
    17161 gtagaaggtg aacgagaaaa actaattaaa ttgaatgaaa cacttcataa acgagtgatt
    17221 ggtcaagatg aagcagttga tgcggtcagt gacgcggtaa ttcgttcacg ggctggattg
    17281 caagacccaa atcgtcctct aggttctttc cttttcctag gaccaactgg tgtcggaaaa
    17341 accgaattag cgaaagcgtt agctgagaac ttgtttgact cagaagatca tatggtacgg
    17401 atcgatatga gtgaatatat ggaaaaacat gctgtgtctc gtctagtcgg agctcctcca
    17461 ggctatgtag gttacgaaga aggtggtcaa ttaacggaag ctgtcagacg aaatccttat
    17521 acaatcgtct tgttggatga aatcgaaaaa gcgcatcccg atgtcttcaa tatcttgtta
    17581 caagtcctag atgacggacg cttgactgat tcaaaagggc gagtagtaga tttcaaaaat
    17641 acggttctga tcatgaccag caatatcggt tcacagttac tgctggaagg cgtgactgct
    17701 gatggaacga ttccagaagc agtggctgaa caagtcaaca cgctattacg tggaaacttc
    17761 aaaccagaat tcttgaaccg aatcgacgac acgattttat ttacaccatt gagcctagac
    17821 aacgtgaaag gaatcgttga caaaatggtg gcgcaattgg cacaacgtct ggaacatcaa
    17881 gaaatcctgc tgacaatcag tgatgaagcc aaaacgtgga ttgctgaaaa tgcttatgaa
    17941 ccagcatatg gtgcaagacc attgaaacga tttatcacta aggaagtaga aacaccacta
    18001 gctaaagaaa tcgttgcagg acatgttatg cctaaatcaa aagtaacgat tactttactt
    18061 gatggacaac ttcatttcaa aacagaagag ctagaagaaa tcgtctaatc ttccattgag
    18121 actataaaag aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa
    18181 cagtgttcca gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt
    18241 ctgtcacaac ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta
    18301 acgctcatct tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt
    18361 atttgtcagc aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc
    18421 atcagaagca gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca
    18481 gaactcccaa aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca
    18541 aggactggtt tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag
    18601 agctgtcagg accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact
    18661 gatcaaatgc atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa
    18721 aacgattgct gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat
    18781 caatgcaatc cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt
    18841 ttgtgtgata aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc
    18901 tatttgcacc tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta
    18961 cttctgaaag caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta
    19021 ttgtatataa gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt
    19081 ttttcgggtg ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc
    19141 ctttcgcata agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta
    19201 gtaaagcaat cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc
    19261 gactgtctgc ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg
    19321 aaagctcaga agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac
    19381 ttttagcaaa cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa
    19441 aaataatcgc taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt
    19501 tcttcatctg tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc
    19561 atcaagcaag tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag
    19621 acacggactt gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg
    19681 acaggatctt caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga
    19741 aggaattctt ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc
    19801 tgactatctt caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag
    19861 tagatcatgg aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca
    19921 agatagaaca tttcttttac gtgaaatatt tcttcagcta cccagtagaa cagaacacct
    19981 cttggttcat ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa
    20041 cgccaatact cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca
    20101 aatttgtcat aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca
    20161 cttactggga tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac
    20221 atgatttccc atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc
    20281 cattgcttat tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca
    20341 tggttagcat attctggata agttccaact ccagtcacac cacccatttt ataacgtgca
    20401 ccgtgaatat tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg
    20461 aaccatccga acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt
    20521 ttattctcaa acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc
    20581 aattcattga attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta
    20641 aaatcttttt gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat
    20701 tctacaaaaa aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt
    20761 tggtataatg gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat
    20821 gaaagaacgc caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa
    20881 atctacattg gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca
    20941 agatcagctg ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa
    21001 tgctgtagaa ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga
    21061 caccccaggg cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg
    21121 tgccgtttta gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta
    21181 tttagcgtta gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc
    21241 agcagatccc gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga
    21301 agctgtatta gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt
    21361 ggaatatgtt cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga
    21421 ctctatttat gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt
    21481 caaacccgga gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt
    21541 cggggtcttc tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta
    21601 tatcacagca agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc
    21661 agacaatcct gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg
    21721 tggtctttat ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact
    21781 gcaattgaat gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg
    21841 gttccgctgt ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg
    21901 ggaattcaat ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac
    21961 ggatggtacg actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca
    22021 ggatgtagaa gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc
    22081 agtaatggaa ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga
    22141 ttaccgagta aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga
    22201 caaattgaaa tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca
    22261 aaaaagcaag ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag
    22321 ctttatcgtt caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa
    22381 aaaactaatc ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat
    22441 cgttgcccgt tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg
    22501 agatgtttct cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa
    22561 acaaatcggc tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggatga
    22621 gcaagatagt gtgaaataac ttataatcaa tcgaaaaacc tattaaataa acctttttga
    22681 gtagatgcaa cactgaggat tatctgtata aaagatttta aactgtcatt tggttatcac
    22741 ggcgatacta aaattaacaa aattggaata tttttcttga agatttttag ctgtcagtat
    22801 atgatgaaag catctcatag gaggtgcttt tttcatggtt gataattcaa gtggaagggt
    22861 tcttaaatct ctaagaaaag agaaaaaact atctcaaaaa aagttagctg atttagcagg
    22921 tatttcacaa agtactttag ttaaatatga aaaagggtca agaaaaatcc ctaaagacgt
    22981 agataatact ctaagcaaga ttctgaatat agaaactcta ataaaagatg aggaagataa
    23041 gattgagatt ctcatcggta aactaattgc atacagagat atgaataagt tacttaacaa
    23101 agagttagct gataatatag gaattagtga agtattactc agttatgtgt taaatagaaa
    23161 gagaaatccg tcaaaagaaa tgcaaaaaaa aatcgatatt tttctattaa gtaatgaaaa
    23221 agaaattttg aaggagatta atcgtgatag tgagatattt agtttatcaa aagacgataa
    23281 aattgttatg ggaaaaagaa tacgagaagt ccgaaagaac cgggaagaga cgcttgaaaa
    23341 atttggtaaa aactttacca tctatacagg caaaaatgtt ataagtagat gggaaaaagg
    23401 tattaatatt ccagatattg aaaaactaat gaatattgcc tatcttggta aggtcactgt
    23461 tccttacctt atgtacggag aagattataa gaatatactt ccaaaagatg aaagagttag
    23521 cgattttaaa aagataaatt cttttagtat gggattaagg atgaggaaaa ttaggaaaga
    23581 ttattatcta gaaagggagg agtttggaaa gcttttttct ccttcaattt caaaatggtc
    23641 catagatagg tatgagaatg gtagagatat acccaatact aatcgaatta tccaatatgc
    23701 ttatatagga aatctttctt tagaattttt gatttacggg atataaaaat agtttacgtc
    23761 gatacttatt aagttatcga cgttttttat tatagaaaaa atttataaaa ggaggtttct
    23821 tgatatgata agaaacaaaa agggtttgtt gaaagttgct ttactggcag cttttttcta
    23881 agtaactttt tgacaagctc aatggcaaca caagcggaag aagtaataca agaactaact
    23941 gttatgaata acagctagat aaaatgggct atgtatgtta tccaacaatt tagtaatcac
    24001 tagaagccta gcgaaagttc gcttgataag ttatagatga gtattgattt gtagtgtttc
    24061 caaatcaaaa gggtacagag ccacatgaaa tatgttgtcc tctgcagcgt caggatggga
    24121 atccatacca tggagattcg gagatctgtg agggaatact tacgtggtta atatgtcagt
    24181 tgtgttggac gtagtaatgg tcatagtggg tagattgact gaattcttat atgagaaaat
    24241 gaaaccaaca agaagatagg gttatttata cagaattggg gatcttttct ggaaaagtaa
    24301 tcggataatg cgagattcgt acggtctttt ttaaatttct ctaaaaaaaa cggcatcgaa
    24361 tgggttgggt gattgataag caatgaatca tagactgata tagaataaaa aatgattcta
    24421 tttatctttt tacatctatc gttttttcaa gtagccgcaa attatatcag cattcaacaa
    24481 tttttgtata caagttctag taaaaggatc tgaagaaaaa gttggttgtt gtgaagataa
    24541 aaaaagaatc aggttttact gagtttttct tcaaaatttg tgttattagg ttaggacaaa
    24601 gataagaaga agtatgaaga tttaaaaaag gaatgaggaa aagatagtaa tttccgatta
    24661 ttggagtttt tttatttata aagaagaagc gaataagttg tgattcctag atttcgcgcg
    24721 tgattaatta atgagcaaaa aatgcggaaa gttttagtgt ttaatacacg aggacaaagt
    24781 ttcttaggta ttgaaggcac acgtgtaacg gttgatgggt ggtgtgattg tttcgaggtt
    24841 ggaaaacatg ttgaacttat ggcatttact gagctaaaag ataagaacaa cgtggaattt
    24901 ttcgagagtg atattggttg ggacgatcat gaagaagttt atggaaaatt tattttgcgg
    24961 aagggaaatt cttatacgaa tggggaagca taagtagcga tttgttagag atcattgata
    25021 ggattgaaat tgtcgggaat atttatgaga atctagagtt atttgaggtg atagaatgag
    25081 tgatggtttt gaacaagtat caataattaa aaatcaagtt cgggagattc ttagaaaaaa
    25141 aggctatttg gtagatagtt attttgaagg agattacgaa acatgggtag gtgtctatgc
    25201 tcgtccagag aataaaccta cctatttaga tcctacgacg tcagaagatg cctatttaca
    25261 aaataggtat cgagtagatg gttttaagca agactttgcg gagtggtttg aatgggaaat
    25321 tgaaaacgga gaagtaaaag aagaataaga gtacttgttg tatcaaaata agccagtaga
    25381 acaaccaaac cagtagctag taatgacaat aatactgagg aatcggatca ttatggtatg
    25441 attgatattt ctcttccaga agatcttaag ccagatcctg taacaattgg caatagtgtc
    25501 atgatttttg attcattaga tgaagctatg tcatatggtt ggaaagaaat taaggatgaa
    25561 ggtagtaatt ggtatggcat gagatttggt gatggtgaag ttgccaaaaa agctggtatt
    25621 ccaattggga aatggacagt tgatttccac gaagcaggtt ctttttttgt tctgtaatta
    25681 tagtgggagg gtaaaatcat tgatgaaatg tgttcagata agagcgccag ataaaaatag
    25741 aagagacagt tcacgtcgtt atcacttttg gtaacggcgt tttgttttat tatataaaat
    25801 tctcaggaaa aggaggaatt cttgtgtatg caaaaacgaa aatgaattgg ttgaaagcac
    25861 tcgcattggt ggctttttct caattctgtt tcttggtggg aactgctcaa gcacatgcag
    25921 aagaaacgac ggctatacaa cctacgatgg aagccattgc ccaagtcgct tctgaagtga
    25981 cagaagtcca accagcagaa gccggggtcc cagctggtga agccactgtt gaacaggccc
    26041 ctgtgacaga agcgccaacg gtcaattcca aaacgactct cgatcagacg attggggaaa
    26101 cacaacaccc agggaatagt cctggtactt cacaaccagt tgaagaaact gcctcaatta
    26161 caacccctgc ttcagaaacg tccactctag aaggagaaaa agctgccgaa gcaacgcctt
    26221 ctgtgcagcc agagtcttct gagaaacaag cggaagaaaa gaaacgaata gaagaaacag
    26281 tggaacaacc agcggctact gctgacaaag aaggaaaaaa tgaggaaaac gttgaaaaaa
    26341 caatctcttc aaccgcagct agtcaaaatc aaccaacgaa accaaacaaa gaatctacgg
    26401 ctagtattga aaatcaaaag aaaaaagagc agaacattcc aaaaacaggt acacctacac
    26461 tgttagtaaa agtaccaacc aatcgttcgg ttcgtgttcg ccgtgcaacc aatgcagctg
    26521 ttttaggtgg tcggaaaatt gattggggcg aaaatattgg tgttacttct agtccgaaca
    26581 atctaaactt cactttaaat tatatggttc gttttgagat taatggacaa gtggcgtttt
    26641 gtattgagcc tggtgtctca gccgcagaag gtcaagttta tacagctact gctttgaaaa
    26701 actatttgaa aaattcaaat gtacggaaaa aaacaagctt gatctcttat ttaggatatg
    26761 tcacaaataa agacaaatcc gatgaacaat acattgcggc acaatttatg atttgggaag
    26821 ctttgggcac aaaaatccca atcaaaaata tccgtgttga ttatgatcgt cgaaaagcag
    26881 aaattcaacg tttgattaat aactttggtg aaaaagcaac ttttaataat caaacacata
    26941 cggtaaaaat tggcgaaacg ttgaaagtaa agaataaaaa tgattttttg aaatatgtga
    27001 aagacattca aaaaccaaaa ggtgtcaatg tatctattca cggcaatgaa gtatggatta
    27061 cagtcacaaa agaggcaccg gataaagcaa aaattcaatt gaataaaatt aaagttcaag
    27121 gtgcgccatt agcctatcgt cgtccaggag ctcaaacaat tggtgtgtta catccaacag
    27181 aacctaatgc gtctgttatt aatttacaaa ttctaaaaca aggaaacatt cgtgtgttta
    27241 aagaagatgc ggaaactggt gtgaaagcac aaggtgcagc ttctttagaa aatgcggtct
    27301 atggtttgta tcaagcagat gggaaaaaga ttaaagaaat taccttgaaa aaaattaacg
    27361 gcaaagtaca ggcagaaatt aaagatcttg atcctggcaa atatgtttta aaagaaatca
    27421 aagcgccaaa aggttatgtg ctttctgatg aagcaattga aattgttgtt gaacctggaa
    27481 aaactgtcaa tgcgaccgca aaagatcaag tgatcaaagg aaccattgat ttagtgaaag
    27541 ttgctaataa agatttagtc gattcaacga atccagataa caaaccaaaa ttagctggca
    27601 ttgaaatcac tttgacgtca aaaacaactg gcaaagtggt gaaaaccgtt gtgacagaca
    27661 atgatggtta tgctactttt ggtaaaaata ccgttgtctt tgatacgtac attctttctg
    27721 aaaccaaagg aaaagaaggg tataaactat ttgaaccact tgaagtcaca atttctgaaa
    27781 atgggcaaac tttccattat gttttagaag ataaagtaat tgaacaacgt cttaaagtgg
    27841 tgaaagtgga tcaagaaaca ggaaaagtga ttccacttgc taatacagaa ttcaagattt
    27901 ttgatacttt agccaatcgt tatgtcacaa tggagattcc aaatgataca gaaacaacgg
    27961 atatctttaa aaccaatgaa aaaggctttt tcctaactaa cggtacactt acttatggaa
    28021 aagatcgcta tcgtttggaa gaaatcaaag cccccgtaaa ttacgttttg aatcaaaacc
    28081 cattagtgtt ctcgattacc aatgatacag aaccgatcaa agtgatccat tttgcgaacc
    28141 gtcaagcacg gaaaagtgta aaattattca agtatgaaga atggaacaag aagaaaacgc
    28201 cgctcgcagg tgtgaacttt accttgtata acggtaaaaa tcaagcaatt ggtgaatata
    28261 ccactgatca aaacggggaa attttagtga aaggtctagt tgccgatgac tattacttct
    28321 tggaaaatgc cccattagaa agtttccaac caaatacaga taaacaagca ttcacggttg
    28381 cattcaaaca aaatgcgcca gatgatgggg aaacgttagt ggtttctgta cgtaattact
    28441 tgattccacc aaaactagaa acacaagcgt caaataaagc cgatggtgaa aaagtggttg
    28501 atccattaga aacgatcgtc attaaagaca gtgtttctta taccaacttg tttgtcggaa
    28561 aagaatacac catttcagga gtattgatga acaaggcaac gaatgcgcca attttacaag
    28621 atggcaaaga aatcacaagt accttggcct ttattgcaga caaagtcaat ggcacaaaag
    28681 accttgaatt tgtggttaat gctagtgttc tgaaaggtca aacgatcgtt gtttttgaaa
    28741 aacttttccg tgatggcatt gaagttgcag cacatgcgga tatcaacgat gaagcccaaa
    28801 gtgtgaaagt gaataatcca accgttcata ccacggctgt ctttgaagac ggattgacag
    28861 tcgcagaccc aacaggattg tttaccttaa acgataccgt tgaaattacc gagattcttc
    28921 caggaaaagc atacttggtc aaagggatct taatggataa agcaaccggt gaacccttgc
    28981 ttgtgcatgg aaatacggta gaaacgagtg ttggctttat tgccaaagca gaaaatgaaa
    29041 cggtgatcgt accatttacc tttgatttga caggtataaa tggtcgtgaa atcgtcgtgt
    29101 ttgaaagtct ttttagaaat gatgaagaac tagcaaacca taaagacatc aacgacaaag
    29161 ggcaaacggt acgagtaact aacccaagtg tacgtacaac ggctacacat aaacccactg
    29221 gcttgaaaga ggttaatcca ttaacgaaag tcaccattac agacgctgtg aaataccacg
    29281 atttggtcgt tggaaaaaca tacacagtca acggtgttct aatggacaaa gtaacggaaa
    29341 aaccagtgct agtagatgga aaagaagtca caagtacctt aacgtttgtg gcagaaacaa
    29401 cggatggtac agtcaatcta gactttattc tagacgcacg tgcgttgcga ggcaaagaag
    29461 tcgtggtctt tgaagatctg tttagagaag gcgtattatt ggcttctcac gcagatatta
    29521 ccgacaatga ccaaaccatc aaaatcagta atccaaccat tacgacgaaa gcgacgaata
    29581 aaaatggcgg aaaagagatc ttggccttgc caaatcaaac tgtgcttgaa tgggtcaaag
    29641 tagacggtct tgcgattggt caaatctatc gcttaattgt tcaaggctac ttaacaccag
    29701 acggcacacc atttgaaggc actcatgcgg aaaaagtctt tgcggccgat aaagaaatga
    29761 tggaattctt atttgaattc ttagtggacg gtcgcaatct tgcaggaaaa ggcttgagtt
    29821 ttgcggaatg gctccaagcg aaaacggaag atgagaaaga aacgtttgaa gaagtggcga
    29881 aacacaatgt agatctaaca aacaaagatc aaacggttca aattgtggaa gcgccaaaac
    29941 caccgcaacc agtgaaacca gaacaacggg ttaaaccagc tactgttgta ccaatgttgc
    30001 caaaaacagg tgaacaaaca agtagtatac ttatggttct tggattagta gtattagtca
    30061 ttgctttagg tataacactt tataaagtac gtaaagccaa agattagtca tgaggaacaa
    30121 gcggttagga tttttaaccg cttgtttgat tgtaatgtag aaatatttag aatttatttt
    30181 ggtttttaag aggaggaaaa actttatggc agaaaaagca ttgcgtatga agaaagagtt
    30241 tttaggtcaa gtatttttat tgggttcacg ggaacctaat ccaccaaagg aaattaatgg
    30301 tgtgtctggg cgatatgttc aattacaagc agagtgttcg cgagaagaac agcctgcttt
    30361 ttttgtttat ctcacagctg aagaggataa aagattacga ttaccagaaa aagaggtgcg
    30421 tatttcattt gaagaagctt attttgtttt tcgtaaagaa cgagtgaata gtcgtgaaac
    30481 aaatgatatg ggacaaacag agcgtcgaac aactggctat caagatgttg gtgtcttttt
    30541 aactggtgta aaaattgggg aggttttgta agatgggatt aaaatttcca aataattatg
    30601 acattacaga agcattagat gaaacttgtt taggagccgt acgctttggc gcattagaag
    30661 agtctgatcg tgtatttgat aatgaaacag gcgattatac gggagaaatt cgatcacagc
    30721 gagcacaatt agtttttgga aatccagatg aaccaaagat tgcacgacga gttaaatttc
    30781 cagcagatgt agatttgact ggttttaaac gacgtgatcc tgtgaaatta attgatccag
    30841 aatatcatgt ggtatatatt ccagcacaag atggcaatcg tgaatattca gatttagtga
    30901 tttatgcgaa aggattagag aaagtagaag ctccaacaaa taatcctaat atgaaacaat
    30961 cacaagaaaa aggtttagat caaaaacaac caagtgttga ttctaaggaa aaagagaaaa
    31021 aataaggttt ttttgaaaga ataggaagga gtcttcctat tctttttatc taagatagaa
    31081 ggaaggggag attttgaatt cagataaaac caatcaaata ctttgagaga ttgtgcttgt
    31141 tatcaaaata tccaacaatt gattgagtat tttggagaca atccaacgat ttttgtacaa
    31201 acaaatgcat atatttgtgc ggttattggg cagtgtatgt ttttacgtga tcttgttcgt
    31261 ttttcattac cgtaccagtg gaaacaagat catcaggaag tgatttggat tcgtatagta
    31321 cgaatatgta atatgtgtgc acacccttat aaaaatcgat taaatctttt tcgattatgt
    31381 ttttttacta aatgtgatat tcctgtatta gttgcttttt gtcaaaagtt tttagcacag
    31441 gaaaagtaaa aacagaaagg aggaatgtga caattgaaag ctcatttttt caagtatcgt
    31501 ggtcggcgaa ttcgctattt caatcgttat ttatggtttt gctattatgg tttctttttg
    31561 tttccatttt tgattctgtt aggttggttt agttggaaag tattttatcc acgtttaagt
    31621 acgttttcga attggcaaat gtatcttatt cctggaataa gtattgtgtt gtatttgttg
    31681 ctggttagtg ggttaatttt agggttgatg cattggtcgc gactaaaaga aggatatttt
    31741 gcctctgttt attggcgtca gttacttgtt cgaatgttaa ttgacaatcg gtttgtttat
    31801 acaaagaaac aagttagtga gaaacaaaca agggaaaaaa tgagattacc aaaagtttat
    31861 ttttatcaaa agaaacaaga attagtggtt tcttttcctt tagatggtgg gcgttttcac
    31921 gaacgatttt taaaaatggg tcatttgtta tctgaagtat ttttgcgtga tttaattcgt
    31981 gaagaacgag aaaaagcccg aatcaattat gtattccttt cagattctgg tcggattcct
    32041 attgatcaat gtatagcaga aaatggacgt attcatttaa taaaaacttt ggattgggta
    32101 tatgatcaat caccaaatat gttgattgct ggtgcaatcg gtggtgggaa aacctattta
    32161 ttgtattctt taattcaagc ttgtttaagt gttgggacag tagatatttg cgatggaaaa
    32221 gcggcagatt tagcagcatt aggtgacgtc gatgtattca aaggacatgt gttttacaaa
    32281 ataaatgaag aaatgattat ttgtttacga aatgcattaa aagaaatgaa tcaacggtat
    32341 gcctatatga aaaccatgaa agatcctaaa tatgaaccag ggaaaaatta tgcttattat
    32401 gggttaccac cgcattttat cttttttgat gaatgggcag ctttttatgg ttcgttggat
    32461 atggaagcga gaaatagagt cgataaattg attcagccat tagtattaaa gggtcgtcaa
    32521 gcagggatgt acattatttt agcaatgcaa cgaccagatg cagaatattt tccaagtggt
    32581 gttcgggata atttaacatt ccgtatttct gttggtcgat tgtctcccat tggttattta
    32641 atgacttttg gagaagattt taaaaataag ccattttaca acaaagatca acctggtcgt
    32701 ggatatgctt ttccaggatt tgatgtacct gtagagtttt ttgcgccatt tgtttacaaa
    32761 gattatcact ttatcaaaga atttgagaaa tttactgaaa tgattgtcta tgattttaca
    32821 gtaaaagagg atagtcaaga tcaaacgatg ggagatgata atcagttgtt acaagaacaa
    32881 ctagagcgcc aaatgtattt agcaaaagat ccaaaaggaa caagtttttc tgatgcgcat
    32941 attttgtaat caactaagga ggtgaagtta aatggcagag agttgttatt atagagtagc
    33001 tacagcaata cgaatgatta atccttctct ttcttccaga acgttttatg attggttgaa
    33061 tcgtattgaa caagtaactg attaccgctt tttgcgcaaa gaacgtgttt ttacaggaaa
    33121 agttattaat caagtattgc taacgaaaaa agatatagaa cgattgactc gattgtatca
    33181 ttatcgagta gatttagaag aggatttaac tttgtcaatt tatcgcgttt tttctcctga
    33241 aaaatatagt gaaataacaa aattagatca tttaattttg tagttattat ctatcgggag
    33301 ggctctagac gtagtctggt agccgcactc tgcctggcgg tgcgcattaa ggctgcgaag
    33361 catgggccta taatgcgcac tcgctagcag cgtgtgcagc tcacaaagag gtcgtgcgct
    33421 tgcgcacggc ctctttgtgt aaagcccccc gatatataat atggggggca gaaataacat
    33481 aacacataga aaaaagtttt ataaaccctt ttgtatcaag tgattatcta aggtcagcag
    33541 ttgtactact tttcccaaaa aacgaggggg actagttgat atatcggtgt ttctaaaggg
    33601 ttaaaaaaaa tcgattttgt tattttataa aaggagcgag aaaatgacac cgattgagct
    33661 aaaaagctac agaaagaagt taagtgcaat tcataatata agtggagata aaaagccgct
    33721 atcacaagca agatttgcgc aattgatggg ctgctcaaaa gattacatta gaaagatgga
    33781 acaagggaaa agaaaaatcc cagaggattt tgaacaacga atcaaggaac gctttcgatt
    33841 gtatatgaaa agcggaatag atcttcgagc aatgattgat tatttgcgtc tttccttttt
    33901 tgggacagac tacaaaacag tgattcaacg tgtattacga atggatctga cagaatttga
    33961 aacacgtcca acgggcttgt atctttttga ctgtctttct gttcgtggga atatctgggt
    34021 gttctggcat tcaaaacgaa ctgtaacaga aaatgtactt gtgcaatttt caggacaagg
    34081 ttgccgggaa tatgaggaag tcatgcgtga acaacaaaca gattggcgaa catggctata
    34141 ttccatttgg aaaaatcaag gaaagttgga agctggttat acacgtgtgc aatgtagccg
    34201 attcgatatt gcgttagatg aactttgggg attggaaacg gaagaacatt tcgatctctt
    34261 tgaattacta gaaaaacaaa aagcaggttt attagaaatg gactttaaga gattccagaa
    34321 tattggcggt tgttttttag atgatggcta ttttcgaagt gaaggattat ctttgtattt
    34381 tggatcgcga aagagtcctc tcttttttaa tttttatgag aaacgttttg aaattgcgaa
    34441 ccgagaaaaa atttcagaat tagaagcatt aacgaaatat ggtatttata atcgatatga
    34501 gttacggtta gccaatgaga aagcaacaca agcagtagaa gcatttattt taggtgattc
    34561 accgaaacgt ttaggagaga ttggcgttgg tttaattaat gcaaaactta ttgtttggga
    34621 tagactggaa agtgatgcac ggattgttga taaacgatgg gcaaaattat ttcgttcgat
    34681 ggaagaattg gattttacag taaagccaga aaaattctca ttagatcgtt cagaacgctg
    34741 gtttttgaaa caagtagctc cgacattaaa gttgttacaa gaagcagatc gtattttagg
    34801 aagagacttt gtacaagaag ggattgataa tgccgaacta aatgacgagc aacaaaacta
    34861 tctagaatgg atgggaacgt caggagggaa aattgcatga atgcatatac gacaacagaa
    34921 tggctgcgcc tgttggattt aaaggctgca atcgaagcat taaatgaaaa gatggttgat
    34981 ttgtcttatt ttcgttttcg agttccttat attgaacaag cggtaaaagc tggacgttat
    35041 caggaaaaag agaactggca agaaattgca cgcttattag aagttcgtaa gggatacgaa
    35101 caagaactcg aagagttgga atttagccga aggaaaggaa ggctagaatt cattcgcttt
    35161 tatcgatttt cattgccaat acctgcgatt ttagcggtta aaaaaggctg cgataaaatg
    35221 aagatatatg aaaactgtgt ggcagcactt agcagcgaaa aacctcttat ggaagaaatt
    35281 tcattggtga ctgtcacttg ggaaatgagt cgccagccaa cagaagaaca gacatatctt
    35341 tctttagaag agattgagat agaattagag gaaattggtc gctatgccac ttgttctacc
    35401 tattgtggat cggtcattag tattgctggt gtgattgtct aaatgaaaaa aattatgatt
    35461 tatgttggtg cgtattggtc acgtgatcca acagtattag aaaatccaga ggcggtacac
    35521 tattgcttgc gccaattatt ctatctgtat aaagaacgat tagaatcgtt gattcgtcag
    35581 ttaccatata ctgacagacg tttagatgaa cttcttctaa ggtatccagc aatgtataaa
    35641 cgacgaaaaa atagattgtt accagaagag tatcccattg aaaaacatga acttgaaggg
    35701 cgatttgtgg cttatttcta tgatgatgtt cgtatgcgct tagtagaaca acgaatggaa
    35761 atagaaaatg atcgctacta ttttatcaat tattgtaaga agcgtaagta tcaagtaact
    35821 gatgattttt atgattgtat tttacaagat ggggaagtga ttctatccaa aattgctcca
    35881 agttttcgtg aacttgtgcc gattgatttt ttgaaaaaat gtcatttgcg gattcttccg
    35941 taaaaaagag ctataaaagg aaagaaaagt agaagccaaa tcggtttctg cttttttatt
    36001 ttttttagga aggagaagat ggtatgtcac tggaattttt aggagagcca attgaaaagg
    36061 aagagttcgt ttcgcattat atggatttgt ttattcaggt gataaaaaac tttgtgcgat
    36121 tgatgaattt tatccacgcg aacaagacta tctagccgct gaacgaataa aaatcagttt
    36181 attatgggaa aaattcacag atgaaaataa ccgaccgcca gattatcgct atttatcaga
    36241 ttgtttaacg aataatgtgc tttctaaaga atacctattt cacaaacgtg agaaaaatca
    36301 aatgtcggtt gaacattttt gtgattgtta tattgcatta ttgcgtagtc aacagttatt
    36361 aacagtagaa cgatatggtg cagagtttgg ttatgtcatg caagctgagc gtcatagagc
    36421 tacaagatgc tatgaaaaag aagatcaagt gattgatggc tatgaagaat tatgtattcg
    36481 aaacaatcgc tatttgcaac aacgtttata tgaacaattg atgatggatt tttcaaatct
    36541 ctatagaggg tatgaatgtt cgatgattca agaaaggtag aagaaaaggg ggaattttta
    36601 tgtggcgatt attatttttg gtggtatgtg gtatggcatt gttctatgtg tatcgtgtct
    36661 atctctatcc acgaatggaa cgatggacaa gttggtatag agatccttta aaacaacgaa
    36721 aaggattgaa gcggtatcgt tatgaattac ttgttgaatt ttcggatggg cgacaagcac
    36781 agtattattt ttttgcaaac tatccaaaat acaacaccag tgaactattg attcaattat
    36841 tcaaaaataa tccatatgtt gggttagaag aaaacggacg ggagttcttt tatcgttcag
    36901 ataatattag tgcggcgtct atgaacaaaa cacgaattgc ataaaaaatt ggaggaataa
    36961 tcatgaaaaa aacaacatta gcagtcgtaa caacactagg aatttcatta ttttcgtttc
    37021 aattagggag ttcattggtt tatgcagatg aaactgtaga aacacctaag tcagaaattg
    37081 tagaaacatt gccaggggaa ggattaccag atgttgagac agagctaaat ggaggaacaa
    37141 cggaaaaacc agcagaacct gaacaattgc agtcagagaa acctgaaaaa gagccagtga
    37201 caccagaacc agagtttcct gaatcaactt taccgactga ttcgatcgaa gaagaaggtg
    37261 tgcatagttc aacaacaacg gattcaagta tagtcacaac tccggaacaa tcaggaacaa
    37321 ccagttcttc tgaaaagaat actgattcgt caagtacgga acagccaacg atgtcgtcta
    37381 ccagtgaaga acaaaaagaa gaaccgaaac aacctgttga aaaatcagaa gcaattccaa
    37441 aagaaaataa acctattgtt ccagcaaaag aagtcacagt atcggtaacc ccaagcggtg
    37501 aaattacgac gaatacttct caaggaatga gtgtaccgat tgtcactagt aatgtagagg
    37561 aattgaccca tattccaaca ccaaccaccc cattaaaagt agcaggtgat caaacaattg
    37621 ttggtgtaaa agatggtatt ccattaatcc aagatagtga agggaatttg gtaaaggatt
    37681 tgtcgattcc tgtaaagaaa cttccaagtg gcaatattga aatcaaaaca gcagatggaa
    37741 aaacaaaagt cttaccaaag actggtgaag aaatacatgt tgcactttct gttctaggag
    37801 gagtgttgac aagtattgct ggatttgttg gatataaaag aaagagaaaa ggaatagaaa
    37861 atgagggtaa ttgaatggac tatttttcag aaaaacaagc ttatcgttgt caatcgtacg
    37921 tactagtaaa aacatactag aaggagtagc ttatgaaaaa acgacaacta cggcggaaag
    37981 aaaaagttta tacagagaaa gtaccgaagc aaaagaaaat ccattcaaaa aaagcagtga
    38041 aatggatttg gtgtttggtt ttatttcttt gtttttcagg gggcttggca ttttttcaat
    38101 ccatgaaaac gaaggttcaa gtgtcgtttt tgaaagatga aatccaacaa ttacaaaaga
    38161 aagaagtaca aagtcggtca gaattaacgt acacacctga aattgaatcc tttatgaatc
    38221 gttttgttgc tttgtatatg aatacattcg ataatcatga acaagaacaa caacgtcaag
    38281 aaacattaaa aaaagagttt tatgcacaaa actttattca agaaccagga agtgtttcag
    38341 gtgttcgaaa attaactgct gcttcgttta ttggattaaa agaaattgaa accattccaa
    38401 ctgctagtta taaagtaact tatgatattc gctcgatgaa tccagagagt aagaaagaaa
    38461 caagtgtgac aatcacacaa gtactcaatc ttcccttttc tatttcaaac aagggctgtc
    38521 gtattattgc ttatccttat tttacggcac taccaaagaa tacagaaaaa caacgaatga
    38581 ttacttatga tgacacatta tacgaaacgg ttgatgcgtc ggaagcgaaa gatgttgaag
    38641 gatttgtgga agatttctta gataagtatg cgaaaaacaa tgcaaaagat atgtcataca
    38701 tgatgaaaga acctgaagga ctcaatcaaa cagctaaagt tgatcaagta gagatgaaag
    38761 tattgcgtta taaagaaacg aaaaaacctt ttattgtaaa agcaacggtc gtctttcgag
    38821 atggtgatac gtcactttta caaaaagaac aaatgacgat tgtcattacg aaaaaaacaa
    38881 atcagtatta tgttgaaaaa ttaacacata attggttgga taggaaagga gaagaacgga
    38941 aatgacactg aagggaattg tagattttat attaacgcaa ggtggctatt ttttaataat
    39001 ggtggttgtt tatctgggat tgcgaaattt tatgaaatca aaagtaggaa atgcattaat
    39061 atctatcgca gcaggtgccg cagtttattt cttcttaaat gatccaacac gtgtatttga
    39121 tgcgtttggt aaaatcattg aaaaattatt tatggattag gaggatagtg aagtggattg
    39181 ggaaccttat gattattcga ttgtttatgc ggaaccttat cgcgtacaac agattacaag
    39241 caattggcgt cttccatttc cagttgcatt agtcaatgca gcaacttttt tgttggtcgt
    39301 aatctttgaa tatttatttt tacgttcatt tattggttgg gtcatttctt tttttccacc
    39361 agcaggagtt gttttttata taggactccc ctatttatgt gtgttagcac tggcaaaact
    39421 aaaacctgat ggaaaaaaag tgcattttta tttatgggat tggttttgtt atatgattcg
    39481 tatgatgaat ggtgattgtg tgtattataa ctgggacaag gtatcaaaca atgcattgga
    39541 agaattactt tttgtgaata agtatgttta tttagaggat tagaaaaagg gacaatcaac
    39601 tggttgttcc ttttttattg ggaggtgaaa ttcattggtg cttgtaaaag aaccagtaaa
    39661 agcattcaag aaaaattgtt tgttaaatgg agaggattgt atctgggcct actatcatgt
    39721 ttttccacga acaatttcaa aaaacacacc aaaaaagaca caggaaataa aagaaatgat
    39781 ggctcgttta ctgttgcttt tagcagatta tgaagatgtg gagttggtga tgtatccacg
    39841 aacaatgaat ttacggacga agtttgacac attaggaaag ggattttctc ccgatagtca
    39901 agcaattggt gcgtattatg caaaagaaac agtgacattg ttggaacgag aaggtcaagt
    39961 ctatcgtcct tcatttattt taggaatcaa agtcaaagaa aaagcaacgg tggagagttt
    40021 tcatgatgta ataaatctaa tctggttaga tttagcagga agtttagcac gcttatttgg
    40081 attagaagta gatgatttgg aaactttgtt ttctgaaagt gatccttatg taaaagaagt
    40141 gacacaatta ctttatagct tttctattcg tcccttagta aaaaaagagc tgttgtatct
    40201 caatcgtctg caatatattc gaggaatggc tcacaaagta gaagaacaaa ataattggga
    40261 agtactgaaa gacaatcaag gaacgtatgt acgtataccg aaggaacata aaggccttct
    40321 tcaattagaa gatgagttag ggagtagtta tttagcattg ttgcctattg gggaaacacc
    40381 tgaaaatatg agaaattgtg atctatttca atttgctcag gatctttctt tcccagcaga
    40441 attgcgaatg aaaattcact atccacgctt gaaaggacag ggaggtattg gtttaaaact
    40501 gaattggcta tcggaacggt tcaaaacaga gcataaggaa ttggttcaaa atgatgaaga
    40561 acctagtgaa cgcttattaa ctgttcgagg attagtaaaa catttaaagc aacgagtaga
    40621 taatgggcaa cctgtcgtcg attggatcgc atgttttatt gtatacggtc aaacggttga
    40681 agaagttcgt caaaatagtg caacggttat tcaacaactt catacgatta tccatatcca
    40741 ccgtgcaaaa aatgatcaac gagaattgtt ctataaatgt ttattagggc aaccattggg
    40801 acggcagtta cattggaagc accgaacaac tgtagaagca gttggcgaat ggttgttttc
    40861 agcagtatca gaattaggta tggaatccgg ctggtatatt ggtcggcaag atacattagg
    40921 ggatggtggt tctcgaggag aaaaaacacc attagaagaa ttaatttatg cgtcaaatcg
    40981 ttttgtcttt ttgaatcttc tagccattgc ggaaggaatc aaaggtgcgt tgtttgatgc
    41041 gcctcatgta gcagtaactg gaaaaaccgg taaagggaag acctttttag ttggcttgct
    41101 ctttttgtat agttcgttta tgaatgtaca aagtttattt gtggatccca aaggagaaaa
    41161 gcgcaaatgg tttacgaaac tcattcaaga cccgtattat cagaaacatt accctttgtt
    41221 tgtagaacat ttgcaaaaaa tgagttatgt cacgttagat gctacaaatc cccgtaatta
    41281 tggtgtatta gatccaattg tttgtttatc ccgagtagat gcgaaacaag tagcccagga
    41341 tatgattgat gagttaagtc cgttaggtac caatcacctt ttaaaagggg cggtattaaa
    41401 gggaatccaa gaagttttag ttcaaaaaga agccggacaa caagttggct tgatgcatgt
    41461 ggtcgactat ttagaacaaa tggaacaaaa agaggttcga gaatatggtg agtttttacg
    41521 cttgacggta gaagattcga ttttacgttt aggattttct tatggagaaa acccgggatt
    41581 agactttaat gccaaaacaa cgattttaga gatccaagat ttgaaattac caaatgacaa
    41641 tgtccgacca gaactatata cagatgcgga tcgaaagtct ttgtgtttga tgattagttt
    41701 aggtcgtttt tgtgaaatgt ttggaaaacg agatagtagt aagaaaacag cgatttattt
    41761 tacagaagca tgggtattta ataattcgaa cgcaggacgt agtattattg cagcaatggc
    41821 acgtgtgggg cgttcccaga tgaatcaatt agtgttagat acacaattta ttggtgactt
    41881 aggttcagat aaagaaaaag ggaattttgg tgtcgtcttt gcttttgatg aagacagtga
    41941 gcgagaaaag attttgaatc atttacgttt ggaagtaaat gaagcgaact tagctgaaat
    42001 gagccacatg attaaaggac agtgttggat gttagatccc tatggacgtg taggaaaact
    42061 caatgtacac agtttgtttg aagaaatgac cgctgcatta caaacaacgg aaaaaacaag
    42121 tcactctcaa gtagaagaac aggatacgtt aatcatgcaa tagaaatgag gtgaaacaat
    42181 ggcttatata aagcataagg aaaagaaatg gtgcgttttg ttttttttaa cgattggatt
    42241 cggactgctt tttgttggtg gattggtgta tgcggcagaa aagcaggtgg atcaatcgat
    42301 ctatgagatt gggaaatttc atattgatac cttttcttct tatttatctg atagtgaatt
    42361 ttcattaggc aaaatgttat ttggggcaaa tggcacagat tttctaaaaa tgatgacgaa
    42421 tatgttcttt agtttaagta aattgatttg gcaaggcctt gattttgtca ttgagaagct
    42481 ctatgaagga gctgcaatga acgagttaat tcaccagttt ttcacttttt cggatactat
    42541 ttattcaaaa atatacgatc ctattggtgc ggggattgtc gtcttgtatg tgttatatct
    42601 atttactctt cgtatattta gagggaaaca ttacgcaaaa atggtgttta ttcgatttct
    42661 cttcgttgtc attttttcaa tggtttggtt tggctatggt agtaacgtac ctagtaaagg
    42721 agaagctttt accaaagggt taaatgattt gtccgtggag gtagaaggct ttattttttc
    42781 tgctacaaat agtctagatg aattggcgac aaaagatgtc aatggtgttt tagtgccaac
    42841 gacacaaaag gaagccattc aaaaagtgcg ccaactttat tataaagcgg ctgttgttga
    42901 tccgtatata ttgttaaatt atgggacaac aaatttagaa gaattgtatc aagcagagat
    42961 tgaaccacaa gaatttctag ccaaggaagc gtcaagtgaa gaattatctg atattgagac
    43021 aaaagtgacc actgctgcag gagaagatag agggaaaaag aaggcttatc gtgtctatat
    43081 tcaaccaact aaagctgttt ataaagcagt cgttggttct atggtgccat tgcttaatct
    43141 aagtattgga ttaccacttg tagtgattgg catgattcgt tttatctttc aaatgttggt
    43201 gttgtgttta ttacttgtga ttcctttttc attactcgct tcttttgttc cagtatttga
    43261 ttatatgctc atgaatacct taaagtcctt tttgggcttg atgtttcaaa aatcttttta
    43321 cagtgtgatt attttggtgg catttttgat ctataacata gtagacgatt taattccaat
    43381 gaatactgtt tttggttttt taggaaatat gtttgtcaaa gcactcctat caattttact
    43441 cattgtaaaa cggaaagatt tattaaagaa actagggtta ggagctgcag atcaaactat
    43501 aaacaacacg aaaaacatag caagtcattc tttgtctcaa atgaaacaac tgcctggtca
    43561 actatcaact gctggacaaa atttggcaat caaaggtgca ggattagcag ggaatgtgta
    43621 tcgagcaacg aatccactcg cttctgcagc gatacatgga tttcaaaagt ttcaacaaaa
    43681 acgtacacca caagaatcgg taaaagggag gcaagtggtt aatccctttg aactaggtgg
    43741 gcgttcaaat caagccttgc cttatagtgg tcgtcatgtt cgttatcatg gggtagagcc
    43801 tgaaatgtct tctaccaaat cgaaaacaga gtggaattct ggtctttcaa atggtttgat
    43861 tccaaattcg tccatgaatt cagtaagacc aaaacaacag gtacaatcat tggcacaatt
    43921 gagtaagcaa caagggaata atcaaagaaa accattgtta aataaaggta tcgcttttgt
    43981 tggtcaatcg attccgttgc cttctggaaa agtacccgtg ccacaattaa cagctgctgg
    44041 ttctctttca caacagattc gccaagtgaa aaatcaggcg aaaaatggga gttatccagc
    44101 aatgcagcag ttattgaacc aatactctgt tcctaattcg tcggtagcca ctctatcagg
    44161 aaatcgttct gtgcaacaac cggggcaacg ttcgtctgaa agtaaaccgt cgatttccgc
    44221 accaaaatcg ttacaacctg cattttcttc tttagataag ccaacagagc aatcggaaaa
    44281 aatagaaaag gatcctaaaa cactgccgat tttggacgca aaaccaggaa ctgattttac
    44341 tttaccaaaa ggacgaacag tccaaaaacc attatcgaag aaaggatgag gaagaaatga
    44401 agaaagggtg tctaggttgt cttcttttct ttattcttcc cttttttcta ttgccattct
    44461 ttttttcagg agaactttct gagcatggag gagaagctac aggtctagct gtcgctttta
    44521 atggggaata taccgaagga ttaccgattt tttcagagat taaaggacgt ggtccaatca
    44581 cagatgaaca tgcgcaagca gcagttggtg cagctgtaaa gtatcagtta ttacggagtg
    44641 tgattctttc tcaattaggc tatgaatcag cttatggaca gtcacaatct ggaagaaacg
    44701 atacgaactt ttttgggatt acctggtttc ctggttgtcc ctacccacaa ggttctgcac
    44761 gtggaattgg tggttctgaa ggtggtttct atatgaagtt tccgaactta aaagcttgtt
    44821 atgactatta tggctttatg gtggcgagcc aaagcaactt taatcaatgt gtgggcaata
    44881 aagatcctgg aagttgttta ttgattctag gtcgtggagg ctatgcggca gcaggtatta
    44941 ccgaagggag tccgtattat cgaggagcaa tgagtatcat aacgtcctat aacttaacgg
    45001 aatatgatac ttttgcgatc aagcattgga atagcctacc aacaaaaaaa acttcgctta
    45061 ttgcaggaaa aagtaaaggc aatgcgaagg ttttggatcg gatcattgga cgatccgtaa
    45121 ataatgggga atgttatggt ggtacggcat ggtatgtatc tcaattaggt ggcccacagt
    45181 taatgggttc tggacacatg tttgcgatgt taatagggga agattataac tggacaacct
    45241 atggttggca agtaatcatg aacccaaaac caagtgattt acaagcagga gatgtggtga
    45301 attggcaagc aggtggtgca ttgtctcctg gaatctatgg tcacaccgga attataacag
    45361 atgtttcata tggcggacaa catttctcta gtgtggaaca gaatgccgag caaggacgga
    45421 ttatggctcg gtatcaacgc acgtacaatc aaacaaaaat tcgtagtctg gttcgtaagg
    45481 tgaaataaga aaggcagtga ctagaaacgt gaaaaaaggg ataattttag tatttagttg
    45541 gttatgtttg ataggtctta ctggttgtca ttggttttct tctgaaaaac aaaaaacaca
    45601 attgagaacg acagaagaaa caatcgaagc agtaaaagaa acaagtggaa ctcttcaaag
    45661 tactgaaagt aacagtcaaa ttggttcatt agaagacgag acagcgcaac aaatgcaaat
    45721 agatgaaatg acaaaaaatt tcttaaaaga ttttgctaga aaatggagta atttcacaga
    45781 tgtttataaa cgcaatcaag ctgtgaggga ctatatgaca gatcgttgta ttaaagacaa
    45841 tagtattgat gtagacccgc atgtacaaat gaaaacaact gggaaacttt accaggtgac
    45901 acaagatata gagaatccgg atcaatattt gttatttgga gaagaaacaa tgaataaggt
    45961 tactcaattt gtattacttc aattgaaagt agatcaagaa gctcaaaaga ttgatgcgat
    46021 caagatttat tatgtacgga gtgcgtatta aaataaaaaa tgaacactca tatttattat
    46081 acaccgaaga aatagaaaaa aacagactgt ttttttaaga aaaactatgt cattctttac
    46141 ttgaaaaaag ttgttatagg acagaaagga taaaacaaaa acggaaaaaa tatgtcttat
    46201 gaataactat aagaactatt ctatttttta ggagatggat acgagatgaa aaaactaatt
    46261 ggctatagtg tgatgtgtat tggatttgtt ggtgtgggat tgtttgtatc gactgaatct
    46321 gcttctgcgg ctgaatggta taatcctatc tggtataatc cgaacattga atgtcaaaaa
    46381 ggctatccgt atggaccgac aaatctgaaa aatggaaaat gtagagtaaa ttggaatggc
    46441 gttgtagata gtgtaattac tggttcaatg gactctgctc tacatggctt tacgggtcac
    46501 tagaaggagg aagaaaatgt ctgaagagaa aaaagaaatc gttgaatcat tagtagcttt
    46561 aaaagattct ctttctaaga ttgaatatgt tgataggcaa gaaattcaaa aactaattga
    46621 tgatactatt attgaaatcc aagatgcgcg ttgtgaaggt ataaaaattt ctgtagcttt
    46681 atctaaagta atagaaaaaa tgaaccgttc attagcgttt aatggattga agctagatag
    46741 acaaactagt ctgatatggg atcatttaaa agacttgtat gacaagtcta aaagaagtga
    46801 acggactgct gtatcaatct taaaaggcct ttggggaatg aattcataaa caagatattt
    46861 aaaaagtaac ttatttttta tggaggagaa attatgaaaa gaataattaa aatatttggt
    46921 gttttaggtg ttttcttatt aggtttaagt acttttgtta gtactggaac aagtgtgtat
    46981 gccacagaaa aagtgaatac agttgattta gaaataaaga ataaattagt agatgaatta
    47041 actttttatt ttgatacagt tggtttttta gacgaacatg gtgtatacca tataaaggat
    47101 tttggtgcta ttcgtgaacg aatcagtcaa gataacgaac aaggaagagc agcaagagtc
    47161 ttttatgaac atattattgt tcctagaatg gagaaagttg attggaaacg atatgctgga
    47221 tgtgtagcca ttaattctct tcctggagga ggagtggctt gggctatagg tacgcaggga
    47281 gattttattg aagcattaaa agcttttaat tttgaaaaag ctgcggattt attattaaaa
    47341 attgcagaaa aacaattagg aaaagctgca gctaaagagt tagctaaggt aaatattgta
    47401 gcaggaattg cagcagctgc tgctagctgt gcattagagt aaaagtttat gaaaagttta
    47461 gaacaagagc atatggcttt taaacaagca atgtttaaag aaaatatcta tttaaaccat
    47521 aactatattc gtgtttctaa ggcatgttca ccagtattga atatgcttgg aggtggaaat
    47581 ggattatatc atttactgtt tgtcgatgtt tgttggttaa tttttttacc agatgaacta
    47641 gttatagtca atgaaaaaat aacaagtaaa aatgaagtgt ttaattattc tttaacaaga
    47701 attaattaca aagagattac caagttttca ctagaaaaag ttcctttttg gggagaattt
    47761 tgtttaaaaa ttaaatgtaa ctggaaaaga atgtatttct atattgatgg tgatgatgct
    47821 cttacattcg gtaaaactac tttttctagt tttaattttc agtttctatt aaaaaataat
    47881 ttttatggtt tattgaaata aggttgcgaa ggaagatgta gagttatttg cactgttgtg
    47941 ttggtatatc tcttaatgct gtgtcttttt ggggagatta attagtggag tgattatatg
    48001 agtatagatg attgtttaga agaacaaata agattatttt taaagttttt aaaagttaaa
    48061 gttcaaaata actacttcac atgttatagt tatgcacacc ctgttttaaa tagtcttcct
    48121 tttaattctt tagtgcaagg attgattaca aatattgttt tattagtctg tacaccaaat
    48181 gaaatatttg taaaaaagat aggcagtggt atttcttcag taagattaaa agagaaagaa
    48241 tatactcaaa agattattca tattcctaag cctgaaatta agcgttttga gataaaagac
    48301 tattctgctt tagggctgtt ctcaattggt tactggtgat aaaaaataag aatactaaaa
    48361 ctatacgtag aatataagta atcggcatca atcctaatta tgtgattgaa tgccgatttt
    48421 tgttacgcgc tatttttaat tatctgtaag aagaataatt ttattttact catattcgtt
    48481 ttcactttga agaagtaaat tgcaatttct tataagttaa gcagctatgc aaaaggctat
    48541 ttattctatt gtgaaatata agatgtagtt gaaacaggat atatagaatc aaaatacgaa
    48601 tcttattttg atttttctag ttttttgaaa aaagcaccct ttgatcgttg tattagtgga
    48661 aaagagaaaa aggagcatac ttatcatgtc acaaaactac aactaaatat ctaaacaaca
    48721 ccaatttgat gcctattgaa aaaaagtatt gcgaaatgaa gccaagtcta ttagaaaatg
    48781 aaataataga ataaaagaaa atgggaaacc gctgaatgat gtaaatgaag gtaagtatcc
    48841 acgaactaat tttgatgaac aagatatcta ttttttattt gtattggaga tactagttta
    48901 atatcaaaag ttatcagcag tcataattag ttatccaatg taaaaagtac aaacaatagt
    48961 tgttatgcag aatagtttat gaaatatatt acataataag aaaacaagcg atatttttct
    49021 gatcagaaca accatttaat ttaaaaagag ttaaaagtta tatatttttg aaatattttg
    49081 gatgagtagc taaaacaata ttttgctcat taagcattaa atgaagatgc aacgatgtta
    49141 gaaagttagc ttcttctgga tcttgttttg ttaatgcctc aaatagttga taatgttgtt
    49201 ccataatttt ttcccacttt aatccttccg tttttactcg tagccatcga aatcgttcta
    49261 agtgtgtatt gttattttct aaccattgcc atacttcttc tcgctctgca atttcaaaac
    49321 aagctttatg gaataaatta tcattatgaa aaaaactacg agcatttttt tgactaacag
    49381 ctttttcttg ttctgaaatt atagtttcta aaatccgttt tttttggtta ttcattttgg
    49441 cacaacactc taacataatt tgtcgttcca aatgttctct gacaaatcga gcatttttgg
    49501 ccgaatctaa atcaattaat gagatataat ttccgctttg aggaattgta tcaacaagac
    49561 cttgttgtcg gagttgaatc agtgcttcac gtattggtgt acgaccaata gaaagtgttt
    49621 cttctaattt tttttctgat actttttttc caggttctaa gtcagcatag ataatttgtc
    49681 ggcgaataga ttggtaagct tgattttgga gactagtttt aattgtcata cacaatgctc
    49741 cttaactttt attttaactg atattttaat tctgttcatg ccatataata tcaaattttg
    49801 aagtaataca ctagttataa aaataaaaga atacatgaaa aaaagaaaaa ataagtttcg
    49861 ataaaatcag tccaaaaaga aggagtattt tatttttctg aataaaaaat acaatcattc
    49921 tgatatggta gaaaaaagca agtttattcc ctttttatat tgacaacggt tacaatgggt
    49981 tttaaaatat aaaatgtatc aaaactaaca tatcagtttg aggaggttaa ttatggtttc
    50041 gattaaaaat tggcaaaatt ctatcgaaga attcaatgaa atgcagattc tactgccgtg
    50101 ttttgatgtc aaagaagtta agcaagaagg ccagcaacat ccagtatggc ttcattttgg
    50161 tggaggaaat ctttatcgag gatttcatgc agaaattgct caatgcttag cagatcaaaa
    50221 tgaattagaa acaggagtag ttgtgtgtga aacatttgat gaagaagtag tggaaaaagc
    50281 ttatcatgac tataacaatg acattttgga aattgttatg agtggagatg gtgaactcaa
    50341 aaaaagactt ttggcagtaa cggctgatag tttatattgc aatccgaaga atatagcgga
    50401 ctatcaaaga gtggttaaat attttgaaga tcctaaatta cagtttacaa cgtttactat
    50461 tactgaaaaa ggttacagtt taaaagattc taaagggcaa ttcttttcgg tagtcaaagc
    50521 tgatatcaaa tctggaccta gcaatcctca acatacgatg agtattgtaa catccttgtt
    50581 atatagcaga tttaatgctg gagagttgcc gattgcgatg gtttctactg ataatttctc
    50641 acagaatggt caacgttttc aagatagtat ttttacgatt gcagaagctt ggcaaaaaga
    50701 aggatatgtt tctgaaaaat ttattgctta tttaaaggat aaagaaaaag tgacttttcc
    50761 ttggagtatg attgatcgaa ttacgccaaa tccttcagag aatgtaaagg aagatctatt
    50821 gatgatgggg ctcgaggatt tagatatcat tcacacaaaa aaacatacca atattgctgc
    50881 tttcactaat actgaggtag ttcattattt agttattgaa gatagttttc caaatggccg
    50941 gcctaaattg gaaaaggctg gtgtgattat gacagataga gagacggttg ataaagcaga
    51001 catcatgaag gttacgacaa gcttgaatcc acttcatact gctttggcag taacaggttg
    51061 tctgctagga tatcaatcaa tttcagcaga gatgcaggat gatgatttgg ttggtttaat
    51121 caaaggaata ggttatttag aaggtttacc agtagttgaa aatccaggca ttattgatcc
    51181 aaaacaattt attgatgaag taattaataa acgtttgcct aatccctata ttccagatac
    51241 accacaacga attgcaacag atacttctca aaaagtagcg attcgctatg gtgaaacaat
    51301 taaaaagtat gtcgaaaaag acggaaatgc agaaaatcta acatttattc ctatcgctat
    51361 tgctgcttgg ttacgttatt tactggcagt cgatgatcaa ggggaaaatt ttgtgccaag
    51421 tcctgatccg ttattggtag agttgcagga taaattatca gtattgaaat taggtgaaca
    51481 agatccttca aagattcatt gtgcggtaca ggaaatttta gaaaataaag aaatctttgg
    51541 tttagattta tatcaagttg gggtaggaaa gaaaactgaa aaaatctttt gcgaaatgct
    51601 agtaggtcct caagcagttc gtaaaacaat caataaatat ataacagaag ttggaggaaa
    51661 gtaactatgg aaatgacttt tagatggtat ggggcaaatg aagaaaaaat ctcactacaa
    51721 gaaattcgtc agatcccagc aatgaaagga attgttggaa cattaatgga tataccagcc
    51781 ggagaggttt ggccgagaga acgaattaaa tctttaaaag atgaagtcga agccaatgga
    51841 cttaccttga aagttattga gagtgttaat gttcatgatg atatcaaaat tggtttacca
    51901 acacgagatc aatacattga gaattataaa cagacgattc gcaatttggc agaatttggg
    51961 attgaggtta tctgttataa ttttatgcca gtatttgatt gggtaaaatc ggatttggat
    52021 tatcgtttag atgatgggtc ttctacgtta gcctatattt caaaaaatat ccctactgat
    52081 ccggaggaaa ttattagaga agtagaaaat tctactaata attttgaatt gccgggttgg
    52141 gaacctcatc ggttatcaca aattaagcaa ttattagaag cctataaaga agttgatgaa
    52201 gagaaattac gagagaactt tgcatatttt ctcaaagcaa ttatcccgac atgtgaagaa
    52261 gttgggatta aaatggcagt tcacccagat gatccaccat attccatttt cggattacct
    52321 cgtattgtta aaaatcgtga cgatttagat tggttgtgta atgtagtgga ttctccttct
    52381 aatggtatta cattatgtac tggaagtatt gcagaacgac cagataatga tgtctatgaa
    52441 attctatctg aattttgtca acgggatcgg attccttttg cacatgttcg taatatcaaa
    52501 tatttaagtg acaatggact cgatttttac gaagcgccgc ataaatcaga atatggttct
    52561 ctagatatgt ataagatatt aaaaacaatg tacgataatg gatttaatgg ctatattcgt
    52621 ccagatcatg gtcggatgat ttggggagaa accggtagac ctggttatgg attatatgac
    52681 cgagctctag gtgcagttta tttaaatggt atttgggaag cgcttgaaaa gggggcgagt
    52741 aaataattat ataagggagt ttatttagag gaaaaatttt ctcttctaaa aactaacgga
    52801 aggaaaggag tgtttaaata tgtttttaga taagaacttt ttattagaga atgaaatagc
    52861 taaaagactt tttcataaat ttgcaaaaca ccaaccaatt atcgactatc attgtcattt
    52921 agacccagca tatttgctag aaaataagca atacaagaat ttgactcagg tatggttaaa
    52981 tgataaagga gctggagatc attataaatg gcgattgatg cgagctaatg gtatagaaga
    53041 agagttgatc agtggtccag gtgatgatta tgaaaaattt ttagcttttg taaaaacagt
    53101 ggagaaggcg ccaggtaatc ctatttttga atggtctcat ttggaattac aacgttactt
    53161 tgatattgat ttgacaattt ctgagaagaa tgcaccagaa atttggcgac gtgctaacga
    53221 aaaaattcaa acggattcct atcgcccgaa agaattgttg gagatgatgg atgtgaaagc
    53281 catctgtaca acggatgatc ctacagatga cttgtggtat catactgagc tttctaaaca
    53341 ggataattct ttcaaggtat tacctacttt ccgtccagat aaggtctgga gtatcaaatc
    53401 atcaggcttt tcgaactata ttttaaaact tagtcaaaca gcggctatac caatcaaaga
    53461 ctttaatggg ttgaagcaag cattgagcca acgagtggat cattttcatc aaattggctg
    53521 tcgattagca gatcacggtt tgaatactta tttttatgaa atagcagatg aaaaaatact
    53581 cgacaatctg ttaataaaag ctttagcagg aaaaacggat ttccatttat cagaaattgc
    53641 acaatttgtt acaggtattc aattacattt gatgtctgag tatacaagat atggttggac
    53701 gatgcaaatg catttaaatg ctttaagaaa taattcaagt atattaaagg aacaaattgg
    53761 agtagatgtc ggtggtgatt cgatggggga tcaagctagt ttgacacaaa atgttgtagc
    53821 attgttcgat accgctgaaa agagtgggtg cttaccaaaa acaattttat attcattaaa
    53881 tccaactgat tggcttccac tagcaacagc aatgcaaagc ttccaagggg gtatgattca
    53941 aaaaatccaa ttgggatgtg cttggtggtt caatgataca gctgaaggaa tgcgtcaaca
    54001 gttgactacg atggcacaac aaagtttgtt gtcgaatttt acaggtatgt tgactgattc
    54061 tcgaagtttt ctaagctatc ctcgccatga gtattttcgt cgagtattat gtcaattact
    54121 aagcgagtgg gttaaagcag gccgaattcc agatgatgaa atgtatctta gtcagatagt
    54181 ggagaatatt tgttatcgta atgcgtataa ttattttaat ttttttgaaa tcggaagtag
    54241 aaaataatac ttgaaataaa agcttaaatt tattctttgt aagttagtac agctagacaa
    54301 agtatgtatc cataaaggaa ggagatagat atgaaaaaag tagaaatttt aagtaagtta
    54361 gaagcggctg gagtcattgc ggttgtacgt gggaaaaata aggatgaggc cattaaagca
    54421 ataaattcaa ttattgcggg tggaattaaa gggattgaac taacttttac agttcctaat
    54481 gctgaagaaa taatcaaaga aattgctaaa aaatatgctg atcaaaaaga tgtaataatc
    54541 ggagccggta cggttttaga tgcgtttact gctcgtatcg caattatggc tggggcagaa
    54601 tatatcgtta gtccatcttt tgatcgacaa acagctgaga tttgtaattt atatcaaatt
    54661 ccttatttac caggttgtat gacaattact gagataaagg aggcacttac tgctggcgta
    54721 gatatcatta aactattccc aggttctgct tttggtccat ccattgtaaa agcctatcaa
    54781 gctccattac cacacgtaaa tataatgcct actggcggag taagtataga gaacttagaa
    54841 gattggtttg aagtaggagt tgttgcagtt ggtgtaggtg ggaatttatt ggcttctgtt
    54901 gaaaaaggtg attttgaaga agtaagtcga gtagctagta aatatataga taagttttat
    54961 gagataaaga attattagga gaaaaaacaa ataatactct gaaatgtaaa aaataaatac
    55021 tgactcttta cgcttcagag tatagtacgt actttcatca gaatgaaaac gcttaacaac
    55081 agaagggaga gaattgtatg gaaagaaaat ttggtataag agacaaaata ggatatatgt
    55141 ttggagattt tggaaacgat tttactttca tttttgctag ttctttttta atgatctttt
    55201 atacaaaagt attggggata agcggagctg ccgttggaac attattttta gtagcaagat
    55261 ttatcgatgc agttactgat gtaactatgg ggcgtattgt tgatagatcg aaagcaacta
    55321 agcagggtaa gtttaaaccg tggattttaa gaatgagtgg tccagtcgct ttagcaagtt
    55381 ttttaatgta tcaaacttct atgatttcag cttctatgag cgttaaggta gtatacatgt
    55441 atgttacgta tatattatgg gggagtattt tttacacatc tataaatatt ccttatggat
    55501 caatggcatc agctattact tctattacag aagaaagaac aatgctatcg acattccgaa
    55561 atgttggagc tactttagca agtttggtca ttggtgtatt aacaccatta tttatttata
    55621 caagagatga ttcaggcgct caaatagttc gtggtggatc aacttttaca attgttgcgg
    55681 gaatattttc gatatgtgca cttatttgtt atttaatatg ttataaacta actactgaac
    55741 gagtaagaat tgaccaatct caatacaaag tttcattgtt aaaatcttta aaagggttag
    55801 ttaaaaatag ggcattactt tctataattg gtgcggctat tttcttactc cttgctcaat
    55861 tattaattat gagtatgaat aattatgtat ttcctgatta ctataatgat gcacagggaa
    55921 tagttttaat gaattttatc aatcctattt tggttttaat agttgtttcg cctttaactt
    55981 tgtttttaag taaaaaatat ggaaaaaaag aactggcatc agtaggaatg ttcttttcag
    56041 caatagtata tttgatctta tttttgataa aagctagtaa tatgtatgta ttcttaattc
    56101 ttagttcggt tggctatatg ggtttgggtg tgtttaatac tgttatttgg gcaaatatta
    56161 ctgatgttat tgatgatcaa gaagtaaagt ctgatcaaag agaagatggg accgtatatg
    56221 cggtatattc ttttgctaga aaaataggtc aagctctagc tggaggcgtc ggaggttgga
    56281 ctctttctat tataggctat gactctttag caaatgtaca aacgggagat gttttagaaa
    56341 agttatataa cgcttcaaca ttaattccat caatttgttt tctgattgta ggattaattt
    56401 tatttttcct ttatccatta tctaaagata aagtcttaga aaattgcgag cttttaaaac
    56461 aaaaacataa ataggagaga aatatgaata attggatatt ttgtattgat tcagatggct
    56521 gtgttatgga tacgatgact tataaacaca atctcttttt cggaccaatt gctgcagaga
    56581 tttttgatgt tcatcataga gaaaaattcc tacaggattg gaataatatt aatttatatt
    56641 cagagactcg tggaataaac agatttttag gtttagttaa aacattagaa aaaaatgatg
    56701 taacatcgat taatcgatta aagcactggg tatctctaac aaaagaattg tctaatccat
    56761 ctttgaagag ggaaatagaa tattctcagt cagaggattt attaaaagct ttaaaatggt
    56821 cggaagaagt caatcaacag attagtcaag caaaaggatt agataaaccc tttttaagtg
    56881 ctagagatac tataaaagct ctgaaaaaat atgggaagat aattattgta agctcagcta
    56941 ataaggaagc tgttcaagaa gaatgggaaa gacatgaatt attatcatta gttgatgaac
    57001 tgtgttgtca agataaagga aaaaaagaag atattattcg aagtgtaatt gaaaatggtt
    57061 gtgatttgga taagatatta atgattggtg attctcctgg tgatctagaa gctgcaaata
    57121 aaaataaagt tttcttttat cctattttgg tcaataaaga aaaagaatca tgggaaaact
    57181 taagaactga tgttttattt aaatgtttat cgggagatta tgtagatatt gaacaaaaat
    57241 atatagatac tt
//
LOCUS       DACDNO010000011        57160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDNO010000011 DACDNO010000000
VERSION     DACDNO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.011"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..187)
                     /locus_tag="IWI22_000710"
     CDS             complement(59..187)
                     /locus_tag="IWI22_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9168122.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWI22_000711"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IWI22_000711"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9168123.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IWI22_000712"
     CDS             complement(1674..2729)
                     /locus_tag="IWI22_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9168124.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWI22_000713"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IWI22_000713"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9168125.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWI22_000714"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IWI22_000714"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9168126.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWI22_000715"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IWI22_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9168127.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWI22_000716"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IWI22_000716"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9168128.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWI22_000717"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IWI22_000717"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9168129.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IWI22_000718"
     CDS             10491..12050
                     /locus_tag="IWI22_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9168130.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IWI22_000719"
     CDS             complement(12161..14092)
                     /locus_tag="IWI22_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9168131.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWI22_000720"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IWI22_000720"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9168132.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IWI22_000721"
     CDS             complement(15021..15773)
                     /locus_tag="IWI22_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9168133.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IWI22_000722"
     CDS             complement(15932..16141)
                     /locus_tag="IWI22_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168134.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IWI22_000723"
     CDS             complement(16152..17480)
                     /locus_tag="IWI22_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168135.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IWI22_000724"
     CDS             complement(17845..18432)
                     /locus_tag="IWI22_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9168136.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWI22_000725"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IWI22_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9168137.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IWI22_000726"
     CDS             complement(20012..21301)
                     /locus_tag="IWI22_000726"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9168138.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IWI22_000727"
     CDS             complement(21455..22387)
                     /locus_tag="IWI22_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168139.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IWI22_000728"
     CDS             complement(22544..23203)
                     /locus_tag="IWI22_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9168140.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWI22_000729"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IWI22_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9168141.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IWI22_000730"
     CDS             complement(24244..24534)
                     /locus_tag="IWI22_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168142.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IWI22_000731"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IWI22_000731"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9168143.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IWI22_000732"
     CDS             complement(26821..28176)
                     /locus_tag="IWI22_000732"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9168144.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IWI22_000733"
     CDS             complement(28200..29369)
                     /locus_tag="IWI22_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168145.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IWI22_000734"
     CDS             complement(29366..30253)
                     /locus_tag="IWI22_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9168146.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWI22_000735"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IWI22_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9168147.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="IWI22_000736"
     CDS             34320..34841
                     /locus_tag="IWI22_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9168148.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="IWI22_000737"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="IWI22_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="IWI22_000738"
     CDS             complement(36284..36940)
                     /locus_tag="IWI22_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168149.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IWI22_000739"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IWI22_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9168150.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="IWI22_000740"
     CDS             complement(38477..39475)
                     /locus_tag="IWI22_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9168151.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IWI22_000741"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IWI22_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9168152.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="IWI22_000742"
     CDS             complement(40491..41222)
                     /locus_tag="IWI22_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9168153.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="IWI22_000743"
     CDS             complement(41226..42491)
                     /locus_tag="IWI22_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9168154.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IWI22_000744"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IWI22_000744"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9168155.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IWI22_000745"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IWI22_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9168156.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="IWI22_000746"
     CDS             47833..49335
                     /locus_tag="IWI22_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9168157.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="IWI22_000747"
     CDS             49337..50074
                     /locus_tag="IWI22_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9168158.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IWI22_000748"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IWI22_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9168159.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IWI22_000749"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IWI22_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9168160.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IWI22_000750"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IWI22_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9168161.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IWI22_000751"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IWI22_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9168162.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="IWI22_000752"
     CDS             complement(55574..56740)
                     /locus_tag="IWI22_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9168163.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="IWI22_000753"
     CDS             complement(56756..56983)
                     /locus_tag="IWI22_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9168164.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
BASE COUNT        15682 a        12459 c        10029 g        18990 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat ccatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgaaggata
    40921 ggaatatccg ttgccgcttg taattcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt tcggctgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gaccggtctt tttcataaaa cacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa
//
LOCUS       DACDNO010000012        55759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDNO010000012 DACDNO010000000
VERSION     DACDNO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.012"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..547
                     /locus_tag="IWI22_000754"
     CDS             <1..547
                     /locus_tag="IWI22_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP9168165.1"
                     /translation="KIYTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSI
                     CSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIV
                     PQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKAD
                     YVTVSNDKDGVSQAFERILTI"
     gene            828..2048
                     /locus_tag="IWI22_000755"
     CDS             828..2048
                     /locus_tag="IWI22_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9168166.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2045..4765
                     /locus_tag="IWI22_000756"
     CDS             2045..4765
                     /locus_tag="IWI22_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9168167.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4749..5693
                     /locus_tag="IWI22_000757"
     CDS             4749..5693
                     /locus_tag="IWI22_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9168168.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5762..6772)
                     /locus_tag="IWI22_000758"
     CDS             complement(5762..6772)
                     /locus_tag="IWI22_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9168169.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6952..7296)
                     /locus_tag="IWI22_000759"
     CDS             complement(6952..7296)
                     /locus_tag="IWI22_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9168170.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7289..7723)
                     /locus_tag="IWI22_000760"
     CDS             complement(7289..7723)
                     /locus_tag="IWI22_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9168171.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7861..8598
                     /locus_tag="IWI22_000761"
     CDS             7861..8598
                     /locus_tag="IWI22_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9168172.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8591..9802
                     /locus_tag="IWI22_000762"
     CDS             8591..9802
                     /locus_tag="IWI22_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9168173.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10009..10788
                     /locus_tag="IWI22_000763"
     CDS             10009..10788
                     /locus_tag="IWI22_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9168174.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10858..11520
                     /gene="trmB"
                     /locus_tag="IWI22_000764"
     CDS             10858..11520
                     /gene="trmB"
                     /locus_tag="IWI22_000764"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9168175.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11618..13372)
                     /gene="ade"
                     /locus_tag="IWI22_000765"
     CDS             complement(11618..13372)
                     /gene="ade"
                     /locus_tag="IWI22_000765"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9168176.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13504..14433
                     /gene="cpaF"
                     /locus_tag="IWI22_000766"
     CDS             13504..14433
                     /gene="cpaF"
                     /locus_tag="IWI22_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9168177.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14390..15439
                     /locus_tag="IWI22_000767"
     CDS             14390..15439
                     /locus_tag="IWI22_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9168178.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15436..15714
                     /locus_tag="IWI22_000768"
     CDS             15436..15714
                     /locus_tag="IWI22_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9168179.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15689..16159
                     /locus_tag="IWI22_000769"
     CDS             15689..16159
                     /locus_tag="IWI22_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9168180.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16176..16478
                     /locus_tag="IWI22_000770"
     CDS             16176..16478
                     /locus_tag="IWI22_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168181.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16495..16866
                     /locus_tag="IWI22_000771"
     CDS             16495..16866
                     /locus_tag="IWI22_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9168182.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16838..17197
                     /locus_tag="IWI22_000772"
     CDS             16838..17197
                     /locus_tag="IWI22_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168183.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17369..18376
                     /locus_tag="IWI22_000773"
     CDS             17369..18376
                     /locus_tag="IWI22_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9168184.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18401..19585
                     /locus_tag="IWI22_000774"
     CDS             18401..19585
                     /locus_tag="IWI22_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9168185.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20022..21605)
                     /locus_tag="IWI22_000775"
     CDS             complement(20022..21605)
                     /locus_tag="IWI22_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9168186.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22183..22326)
                     /locus_tag="IWI22_000776"
     CDS             complement(22183..22326)
                     /locus_tag="IWI22_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9168187.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22549..23028)
                     /locus_tag="IWI22_000777"
     CDS             complement(22549..23028)
                     /locus_tag="IWI22_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9168188.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23101..23385)
                     /locus_tag="IWI22_000778"
     CDS             complement(23101..23385)
                     /locus_tag="IWI22_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168189.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23463..23657)
                     /gene="ssrS"
                     /locus_tag="IWI22_000779"
     ncRNA           complement(23463..23657)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWI22_000779"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23729..25015)
                     /locus_tag="IWI22_000780"
     CDS             complement(23729..25015)
                     /locus_tag="IWI22_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9168190.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25153..25824
                     /locus_tag="IWI22_000781"
     CDS             25153..25824
                     /locus_tag="IWI22_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9168191.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25790..26278
                     /locus_tag="IWI22_000782"
     CDS             25790..26278
                     /locus_tag="IWI22_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9168192.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26291..27238
                     /gene="prmA"
                     /locus_tag="IWI22_000783"
     CDS             26291..27238
                     /gene="prmA"
                     /locus_tag="IWI22_000783"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9168193.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27239..28003
                     /locus_tag="IWI22_000784"
     CDS             27239..28003
                     /locus_tag="IWI22_000784"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9168194.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28236..30449
                     /locus_tag="IWI22_000785"
     CDS             28236..30449
                     /locus_tag="IWI22_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9168195.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30460..30906
                     /locus_tag="IWI22_000786"
     CDS             30460..30906
                     /locus_tag="IWI22_000786"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9168196.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31077..32336
                     /locus_tag="IWI22_000787"
     CDS             31077..32336
                     /locus_tag="IWI22_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9168197.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32425..33372)
                     /locus_tag="IWI22_000788"
     CDS             complement(32425..33372)
                     /locus_tag="IWI22_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9168198.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33766..35058
                     /locus_tag="IWI22_000789"
     CDS             33766..35058
                     /locus_tag="IWI22_000789"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9168199.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35073..36842
                     /gene="aspS"
                     /locus_tag="IWI22_000790"
     CDS             35073..36842
                     /gene="aspS"
                     /locus_tag="IWI22_000790"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9168200.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36951..37826
                     /locus_tag="IWI22_000791"
     CDS             36951..37826
                     /locus_tag="IWI22_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9168201.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38092..39975
                     /locus_tag="IWI22_000792"
     CDS             38092..39975
                     /locus_tag="IWI22_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9168202.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40050..40334)
                     /locus_tag="IWI22_000793"
     CDS             complement(40050..40334)
                     /locus_tag="IWI22_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9168203.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40324..41058)
                     /locus_tag="IWI22_000794"
     CDS             complement(40324..41058)
                     /locus_tag="IWI22_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9168204.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41187..41810
                     /locus_tag="IWI22_000795"
     CDS             41187..41810
                     /locus_tag="IWI22_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9168205.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42002..43123
                     /locus_tag="IWI22_000796"
     CDS             42002..43123
                     /locus_tag="IWI22_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9168206.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43130..46258
                     /locus_tag="IWI22_000797"
     CDS             43130..46258
                     /locus_tag="IWI22_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9168207.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46277..49471
                     /locus_tag="IWI22_000798"
     CDS             46277..49471
                     /locus_tag="IWI22_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9168208.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49617..50324)
                     /locus_tag="IWI22_000799"
     CDS             complement(49617..50324)
                     /locus_tag="IWI22_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9168209.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51089..51487
                     /gene="spxA"
                     /locus_tag="IWI22_000800"
     CDS             51089..51487
                     /gene="spxA"
                     /locus_tag="IWI22_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9168210.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            51819..52472
                     /locus_tag="IWI22_000801"
     CDS             51819..52472
                     /locus_tag="IWI22_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9168211.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            52698..53696
                     /locus_tag="IWI22_000802"
     CDS             52698..53696
                     /locus_tag="IWI22_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9168212.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            53872..55680
                     /gene="pepF"
                     /locus_tag="IWI22_000803"
     CDS             53872..55680
                     /gene="pepF"
                     /locus_tag="IWI22_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9168213.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18324 a         9658 c        11582 g        16195 t
ORIGIN      
        1 caaaatatat acaaatctcg gaagttatga atctcagaat tataaaaata aaatgaaaaa
       61 aatttttcac gaggtaggga gtgaattttc tatcaaagag ctaaactatg agcttgagta
      121 tgaaaaatct atttgttcaa tcccttataa taatattgag gaaattatag aaaaggatgc
      181 tatcagcatt tacaaatttt ttatttttat tcctaatcgt caattgaaag ctaatatatt
      241 taaccatttg aaaaagatgc taaatgtgtc tgttactgaa tcggcacctg taaatattga
      301 aatcgtacct caaaatgtat ctaaaggtgt ggtgtttgat cgtttggaat caatctacaa
      361 tttaaagaaa cctttgagaa tcgcgatcgg agatagttta aatgacttat ctatgtttga
      421 aagtgcagat attagttttg caatgggaaa tagtcatcaa gaaatcatga aaaaagctga
      481 ttatgtcact gtttcaaatg ataaagacgg agtatctcaa gcatttgaaa gaattcttac
      541 catataaact aattaaggta gtatagaaca tagaattcta aatacggaaa aatggagcat
      601 aaattccagt acaaatagta cttcttcagt gtcaaacgaa gcgaatataa catagatatt
      661 taggattgtg acaaaaatca tgtcacaatc tttttttcga ataaacttat tttttgaagt
      721 cggccattta tcgtaatttc tttgcataat acgaacaaat tttcggttat tgatgaaaaa
      781 aaacattgaa aatggtatga taaaagaaaa aaaggaggaa atttttaatg gtgaaattta
      841 tccatgcggc agatttacat atggatcggt catttgaagg actgacaact cttgataaaa
      901 ctgtacagga aaaactattg aagacgaatc tgaccgtttt atcaaatatc attgaacagg
      961 caataataaa taacgttgat tttgttttac ttgcaggaga taattttcat caaaatcgtc
     1021 cttcattaaa aatacagaaa catttttcag aacagatgaa acggttagaa aagaaccata
     1081 tacctgtatt tataatattc ggaaatcatg atttctatca aaaagagcgg tattggttct
     1141 cctttcctaa aaatgtccat ttatttacat ccgaaacggt agagacaaaa aaaataacga
     1201 taaaaagtgg agaaactgtc tctctcagtg gatttagtta tcgtcaaccg tggattcaaa
     1261 aagacaaagt gatggaattt ccttctagag aactcacaga ttaccatatt gggttgtacc
     1321 acggggaacc tggcgtctct caaaaaggaa actatgctcc tttccaaccc tcaaagatgc
     1381 aagaaaaggg atacgattat tgggctttag ggcatatcca tgtgccaacg gtcttaaatg
     1441 aaaagcaaac aattgtttat ccaggtgcgc ctcaaggtca tactaaaaag gaacaagctt
     1501 ctacatctat tctactagtt gaattaagta aaggaagttg ccaaatacat ccgattaaag
     1561 tagcagaggt ctattggaag acaaaggaaa tatcattgag gaaagcaaga acgacaaaag
     1621 aggtcattgt tcacattcgt cagcagcttt caaaagtaga ggatggattt agtttaattg
     1681 agataaaatt aaaagattat gaccatctca atacagatgt tttggaacgg atacaatcag
     1741 gagagctatt agactatttg ttagaggaat tctcagatag gataaatgat ttctttattt
     1801 ggcgtatttc tctcatagag aatacttttt atacgaagac cccacttgct gcttctgcta
     1861 aattgatgga acagttattt caatactatc agacacctca agatttccaa caaattttaa
     1921 atgaagtata tagtcatcaa gaagcggcta gaatattgag cgaactgccg gaatatcaag
     1981 aagaaacact tgaaaaagcc aagcagctgc ttaatcaaga ttttttattt gaggaggatc
     2041 aggaatgaag attttaagga tcgagattgc tgcttttgga aaatggcgtc aaaaaagttt
     2101 tgacttttat tcaggcaatc agctgatcta tgggggaaac gaagcaggaa aatcaacgat
     2161 ttatcaattt attcaagcaa tattatttgg tttcccttca aaaggccgta aaaaaaagga
     2221 ttatacacca aaagacggct cagcctatgg cggcaaaata tggcttaaac atcctgtata
     2281 tggtgaattt gctgtcgagc gctacaagca gcaaaatcgc ggaaaatcga aggtgtggct
     2341 gggggatcaa gtgggaagtg atgagttact agaaaaactg atcgcacctt tgacgaaaga
     2401 gttattccag caggtattta ctttccagca agagcagttg atggaactag atcatttgag
     2461 agaaaaagag ctacatgatg cgctgatttc tttaggcatc acgggcagtt ctcaagtatt
     2521 ccaaaagcgg caagaatatt atcagaaagc acaacaatta ttcaagaaaa aaggccaaaa
     2581 attactaatc aatcaaaaat taaatgaatg gaaagagctg caagaaaaaa ttcatcaaaa
     2641 acaagaacag gaagcttatt ttggcaagtt gatccaacaa gaacaggaat acgagtcgca
     2701 acagcagcag ttattggcac aattaaaaga ggcgaacaaa caattttcat tagccagaca
     2761 gcaagaactt aatttttctt tatatgaaga atggcaatct ctcaaaaaaa acagcactac
     2821 aggtcttcct gatgagcaac ttcttttaga tctggagtct ttctccaaac ggtatcagca
     2881 aacaaatgag cgtttggaag aattagaagc aacactggaa aagcatagcg ggatggatca
     2941 gcagtcagcc agatattatt tttatttaga gcaggagcag gaactaaaag atctccagca
     3001 acttcaattt acagcggaac aattaaacga agaatggcaa agattacaaa ttgagactag
     3061 agaaacaaat tcagaattga agattttgga acaaaggtgg cattggcaga aaaatccgtt
     3121 accggaagaa tttttatatg aagaagaatg gaagcatctt ctggctgaag aaagaaggat
     3181 tgctgatcta gaaagccaga tttctgtgcg tctccaaata gctaaagaac agcaagagat
     3241 gattgaagca gaagtgaatg acttggaaga gacctatcca gcattattac aaaatcagtc
     3301 agagatagca ccagtctctt catctaaacc gtatatgctg atggctggcg gagcaataat
     3361 cattgcaggt atatttgtac caatgccctt gaaagtgatc cttttactaa ttggtgcagg
     3421 catgattggc tatttcttct acaaaaggca accaaaaatt aaaactaaaa atgatttctc
     3481 tgagataaaa ggaatgtggc aagaaaaact aggacaactt gatctagcct tagctcaatt
     3541 agctgaagca gaagaaaaac aaaagagaac tggagaagaa aaacagattc ttcagcaaga
     3601 agtatatcgt tttgcggatc atcggcactt aggaaagatg gatacgctca aactcatgaa
     3661 agaatatgga caggaagtca tagattatca gaaattatat ggcgtatttg agcggaaaca
     3721 taaaagagaa caggaaatac aagaaaaact acgacaaatc gaccaaaaat ttgagtttgt
     3781 taaagaatgg ttaccgatac aagataagac aatttcagaa aaaatggtat tgctttcccg
     3841 ttttatacaa gagatggaac agatgaaatt tgccagaagc tatcagcaaa atactttatt
     3901 gaagcaagaa atcaatcaat tgaaaaagaa acaaaaagta tggatacaag aatacagcga
     3961 acaattgact caagcaggct tatcttatcc ttcggaagta cctgtctttt tacaaaaagc
     4021 caaacaagcc gaacagcaga tgaaaagata caatgaatta tcggaaatgc ttactccact
     4081 atttcctgaa gagctgacaa aagagaaatt atctgtaaga ctgcgccaac tgaaagaaaa
     4141 gcaatcccaa ctccagcaag aagaaaaaga agcaagtgaa aatcggcaac gactccaact
     4201 tcaagtagaa gagttgcaaa gagacggcac attggacgaa ctttatcaac aagctagccg
     4261 gcaaaaagca aaattacagg aattactgga ggaatgggga gcacttgaaa tcgccgggac
     4321 attcttaggt gatctttcaa cagagatgag tgaaagacaa ttaccccagt tgttaagagc
     4381 agcaggtgat tatttaggta ttttaacaaa aggaaattat cagaagatat tactgaaaaa
     4441 tgatacactg actttaacag acggatatac agaatttccc atctatgagt tatctacagg
     4501 tacgaaagat cagctgatta tggcgatgag atttgcttac ctctcattag aagctaaccg
     4561 ttcgatatgc ccgatcatca ttgatgatgg ctggcttcat tatgatcatc agcgaaagta
     4621 tcaattagct gaacttttag ctaaatttgg agaaaagtat caaataattt gtttttcttc
     4681 tgataaagaa atggtaagct attaccatga gatgaaccaa atcgtaaaac aattagaagg
     4741 agcaaataat gaaaaaaatt cgtgatttag taatcgatga gcagtttgaa caatttgtcc
     4801 tgatcaaaag tgcagatgta agaatcgcta aaaacggaaa aaaatttatt gcttttactt
     4861 tccaagatac ttcaggtact atcgatggta aatactggga tgcttcggag gaggaaatca
     4921 ataaatttac agccggcaca gtcgtttttc taaacggtaa aagagaggtt tatcaaggaa
     4981 accctcaagt aaagattctt catatgcgct taacaagggt agatgagcca ggagagccta
     5041 gtttatacat ggaaagagcc cctttgaaga aagaagaaat ggaagaagaa atcaatcaat
     5101 tgttgtttga gatcaccaat gcgcattgga atcgtattgt tcgctatcta cttaataaat
     5161 atcaaaaaca attttttgag tttccggcag caaaaagaaa ccatcatgca tttgccagtg
     5221 gattagctta tcatactttg acaatgcttc acttagggaa atcaatcgcc gatgaatatc
     5281 ctgagctgaa tcgagcattg ttgtatgctg gtatcatttt gcatgattta ggaaaggtga
     5341 ttgaactttc cggtcctatg tcgacagaat atacgcttgc aggaaatttg atcggtcatc
     5401 tggtattgat tgacgaagaa ataactaaag cctgtcttgc gttgaaaatc agcgaagctg
     5461 acgaagatgt tctcgtatta cgtcatatgg ttttagcaca ccatggctta ttagaatatg
     5521 gctcacctgt acgtccaaga ataatggaag cagaaatatt acatcaaatc gacaatatcg
     5581 atgcttcgat gcagatgatg ttgacttcga ttcgtcaaac agaaccagga aaatacaccg
     5641 atcggatttt cggtatggac aaccgaagtt tctatgtacc aaaagattta tagcaaaaat
     5701 ttattttttt cattcaaaac aaaaaaagcg agacataagt cttcgctttt tttgttttct
     5761 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
     5821 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
     5881 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
     5941 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
     6001 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
     6061 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
     6121 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
     6181 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
     6241 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
     6301 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
     6361 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
     6421 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
     6481 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
     6541 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
     6601 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
     6661 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
     6721 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     6781 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     6841 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     6901 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     6961 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     7021 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     7081 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     7141 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     7201 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     7261 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     7321 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     7381 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     7441 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     7501 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     7561 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     7621 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     7681 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     7741 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     7801 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     7861 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     7921 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     7981 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     8041 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     8101 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     8161 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     8221 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     8281 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     8341 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     8401 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     8461 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     8521 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     8581 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     8641 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     8701 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     8761 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     8821 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     8881 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     8941 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     9001 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     9061 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     9121 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     9181 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     9241 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     9301 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     9361 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     9421 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     9481 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     9541 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     9601 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     9661 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     9721 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     9781 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     9841 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     9901 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     9961 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
    10021 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
    10081 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
    10141 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
    10201 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
    10261 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
    10321 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
    10381 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
    10441 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
    10501 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
    10561 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
    10621 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
    10681 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
    10741 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
    10801 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
    10861 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
    10921 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
    10981 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
    11041 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
    11101 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
    11161 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
    11221 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
    11281 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
    11341 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
    11401 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
    11461 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
    11521 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
    11581 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
    11641 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
    11701 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
    11761 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
    11821 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
    11881 gctgtttgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
    11941 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
    12001 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
    12061 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
    12121 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
    12181 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
    12241 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
    12301 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
    12361 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
    12421 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
    12481 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
    12541 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
    12601 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
    12661 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
    12721 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
    12781 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
    12841 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
    12901 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
    12961 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
    13021 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
    13081 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
    13141 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
    13201 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
    13261 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
    13321 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
    13381 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
    13441 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
    13501 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
    13561 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
    13621 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
    13681 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
    13741 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
    13801 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
    13861 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
    13921 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
    13981 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
    14041 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
    14101 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
    14161 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
    14221 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
    14281 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
    14341 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
    14401 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
    14461 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
    14521 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
    14581 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
    14641 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
    14701 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
    14761 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
    14821 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
    14881 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
    14941 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
    15001 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
    15061 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
    15121 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
    15181 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
    15241 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
    15301 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
    15361 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
    15421 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
    15481 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
    15541 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
    15601 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
    15661 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
    15721 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    15781 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    15841 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    15901 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    15961 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    16021 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    16081 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    16141 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    16201 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    16261 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    16321 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    16381 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    16441 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    16501 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    16561 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    16621 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    16681 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    16741 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    16801 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    16861 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    16921 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    16981 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    17041 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    17101 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    17161 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    17221 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    17281 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    17341 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    17401 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    17461 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    17521 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    17581 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    17641 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    17701 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    17761 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    17821 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    17881 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    17941 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    18001 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    18061 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    18121 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    18181 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    18241 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    18301 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    18361 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    18421 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    18481 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    18541 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    18601 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    18661 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    18721 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    18781 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    18841 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    18901 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    18961 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    19021 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    19081 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    19141 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    19201 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    19261 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    19321 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    19381 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    19441 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    19501 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    19561 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    19621 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    19681 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    19741 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    19801 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    19861 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    19921 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    19981 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    20041 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    20101 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    20161 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    20221 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    20281 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    20341 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    20401 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    20461 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    20521 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    20581 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    20641 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    20701 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    20761 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    20821 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    20881 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    20941 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    21001 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    21061 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    21121 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    21181 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    21241 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    21301 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    21361 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    21421 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    21481 gatttgccag ttccgttgat accgactaag gcaatttttt caccagcttc aatttgacag
    21541 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    21601 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    21661 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    21721 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    21781 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    21841 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    21901 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    21961 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    22021 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    22081 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    22141 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    22201 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    22261 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    22321 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    22381 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    22441 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    22501 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    22561 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    22621 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    22681 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    22741 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    22801 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    22861 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    22921 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    22981 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    23041 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    23101 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    23161 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    23221 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    23281 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    23341 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    23401 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    23461 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    23521 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    23581 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    23641 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    23701 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    23761 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    23821 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    23881 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    23941 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    24001 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    24061 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    24121 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    24181 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    24241 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    24301 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    24361 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    24421 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    24481 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    24541 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    24601 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    24661 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    24721 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    24781 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    24841 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    24901 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    24961 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    25021 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    25081 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    25141 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    25201 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    25261 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    25321 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    25381 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    25441 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    25501 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    25561 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    25621 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    25681 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    25741 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    25801 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    25861 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    25921 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    25981 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    26041 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    26101 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    26161 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    26221 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    26281 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    26341 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    26401 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    26461 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    26521 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    26581 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    26641 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    26701 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    26761 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    26821 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    26881 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    26941 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    27001 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    27061 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    27121 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    27181 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    27241 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    27301 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    27361 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    27421 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    27481 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    27541 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    27601 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    27661 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    27721 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    27781 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    27841 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    27901 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    27961 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    28021 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    28081 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    28141 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    28201 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    28261 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    28321 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    28381 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    28441 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    28501 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    28561 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    28621 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    28681 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    28741 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    28801 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    28861 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    28921 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    28981 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    29041 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    29101 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    29161 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    29221 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    29281 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    29341 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    29401 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    29461 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    29521 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    29581 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    29641 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    29701 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    29761 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    29821 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    29881 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    29941 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    30001 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    30061 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    30121 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    30181 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    30241 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    30301 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    30361 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    30421 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    30481 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    30541 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    30601 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    30661 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    30721 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    30781 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    30841 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    30901 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    30961 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    31021 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    31081 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    31141 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    31201 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    31261 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    31321 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    31381 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    31441 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    31501 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    31561 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    31621 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    31681 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    31741 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    31801 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    31861 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    31921 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    31981 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    32041 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    32101 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    32161 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    32221 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    32281 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    32341 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    32401 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    32461 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    32521 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    32581 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    32641 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    32701 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    32761 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    32821 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    32881 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    32941 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    33001 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    33061 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    33121 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    33181 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    33241 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    33301 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    33361 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    33421 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    33481 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    33541 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    33601 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    33661 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    33721 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    33781 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    33841 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    33901 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    33961 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    34021 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    34081 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    34141 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    34201 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    34261 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    34321 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    34381 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    34441 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    34501 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    34561 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    34621 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    34681 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    34741 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    34801 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    34861 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    34921 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    34981 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    35041 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    35101 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    35161 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    35221 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    35281 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    35341 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    35401 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    35461 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    35521 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    35581 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    35641 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    35701 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    35761 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    35821 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    35881 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    35941 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    36001 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    36061 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    36121 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    36181 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    36241 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    36301 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    36361 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    36421 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    36481 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    36541 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    36601 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    36661 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    36721 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    36781 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    36841 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    36901 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    36961 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    37021 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    37081 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    37141 taccggtatc aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa
    37201 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    37261 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    37321 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    37381 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    37441 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    37501 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    37561 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    37621 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    37681 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    37741 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    37801 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    37861 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    37921 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    37981 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    38041 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    38101 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    38161 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    38221 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    38281 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    38341 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    38401 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    38461 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    38521 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    38581 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    38641 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    38701 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    38761 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    38821 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    38881 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    38941 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    39001 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    39061 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    39121 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    39181 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    39241 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    39301 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    39361 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    39421 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    39481 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    39541 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    39601 aaattaaagg aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc
    39661 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    39721 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    39781 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    39841 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    39901 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    39961 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    40021 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    40081 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    40141 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    40201 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    40261 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    40321 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    40381 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    40441 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    40501 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    40561 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    40621 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    40681 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    40741 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    40801 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    40861 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    40921 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    40981 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    41041 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    41101 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    41161 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    41221 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    41281 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    41341 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    41401 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    41461 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    41521 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    41581 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    41641 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    41701 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    41761 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    41821 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    41881 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    41941 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    42001 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    42061 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    42121 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    42181 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    42241 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    42301 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    42361 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    42421 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    42481 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    42541 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    42601 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    42661 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    42721 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    42781 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    42841 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    42901 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    42961 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    43021 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    43081 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    43141 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    43201 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    43261 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    43321 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    43381 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    43441 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    43501 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    43561 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    43621 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    43681 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    43741 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    43801 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    43861 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    43921 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    43981 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    44041 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    44101 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    44161 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    44221 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    44281 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    44341 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    44401 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    44461 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    44521 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    44581 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    44641 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    44701 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    44761 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    44821 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    44881 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    44941 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    45001 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    45061 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    45121 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    45181 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    45241 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    45301 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    45361 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    45421 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    45481 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    45541 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    45601 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    45661 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    45721 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    45781 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    45841 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    45901 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    45961 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    46021 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    46081 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    46141 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    46201 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    46261 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    46321 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    46381 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    46441 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    46501 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    46561 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    46621 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    46681 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    46741 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    46801 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    46861 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    46921 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    46981 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    47041 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    47101 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    47161 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    47221 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    47281 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    47341 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    47401 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    47461 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    47521 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    47581 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    47641 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    47701 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    47761 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    47821 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    47881 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    47941 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    48001 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    48061 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    48121 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    48181 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    48241 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    48301 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    48361 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    48421 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    48481 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    48541 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    48601 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    48661 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    48721 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    48781 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    48841 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    48901 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    48961 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    49021 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    49081 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    49141 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    49201 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    49261 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    49321 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    49381 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    49441 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    49501 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    49561 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    49621 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    49681 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    49741 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    49801 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
    49861 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    49921 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    49981 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    50041 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    50101 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    50161 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    50221 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    50281 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    50341 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    50401 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    50461 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    50521 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    50581 agatttattt ttttaataca gtctcaatat aactattcaa acataaaata acgttatttg
    50641 atttatttgt ttatagtaaa ttttatttag taatcattgc tttaaaatac tacgcttata
    50701 taacagagaa aataagcgtt ataataaaga acagtattat ttaaaatgac agaaaacatt
    50761 actattaaag aaataaaaaa cataaaaaat cttaagaaat cggtttcatt tagcttttga
    50821 gataaaataa tagtacagtt ttatacgctg tttcatatgt ttagaaaatt tgaattagat
    50881 ttgaaaactc taatttttaa atgataaaaa aagcgagaat gctggtttta ctgtatatta
    50941 accttagata actttcattt ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt
    51001 tgattatgta taatagaaac agatgggaga ttattatgat cttctgttac aaaaaagtga
    51061 ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta tatacttccc caagttgtac
    51121 ttcctgcaga aaggctcgtg cgtggttgca agaacatcag attccatttg ttgaacgtaa
    51181 tattttttca gaaccattga acagttctga attaaaagcg attttacaaa tgacagaaga
    51241 cggaacagaa gaaatcatct cgacacgttc caaagtattc caaaaactga acatggattt
    51301 agatgatctg ccgttacaag aattattgga acttgtgcaa aacaatcctg gattgcttcg
    51361 ccgtccaatc atgattgatg ataaacgtct gcaagttggt tttaatgaag atgaaattcg
    51421 tcggttccta ccgcgcgatg tacgacaatt agaattacgt caagcacaat tgatggctgg
    51481 attatagaag gataatacga aacttggaaa agactattac aaagatagtt tttccaagtt
    51541 tttttgtttt tacaaaaagt attcttgatt agatgtataa aaaggtagta gaagatgatg
    51601 attgcacgaa aaatagagag ggaaaattag ctgaaaagtc ggctatatag tattcgtctg
    51661 ccaaaaatgt tatagttaaa taagatagct tgaaaggttg aaaaatccgc tggttttatt
    51721 aaattcgctt aacagcgaga ttttttttct ttacatttaa gctgatttcg ctacaatgaa
    51781 tctatattaa acattgtggc aaagtgaggt gtagcaaaat ggaaatggaa catattaatg
    51841 aaaatacgat ccgtgtacta attggtcatg aagatttaga ggaacggggc gtttcatttt
    51901 tggatttact tggcaatcac aaagaaatcg aaaatttctt ttatagtatt ttggaagaag
    51961 ttgatatcga agaggagttc cgaggaagcg aagctgtcac tttccaagtt ttgccaaaag
    52021 gagacggatt agaactgttt atcagtaaaa atcttccgga tgaagaaatt tctcaatcag
    52081 aagattctac tgttgattca gaagatgtga gtgactttct tcgtcagcag attattggca
    52141 gcgatccgga ggaagaatta tctggaagcg ccctagaaga acaggaaaga caagcaactt
    52201 ttgcttttga agattttgaa caagtgatcc aacttgcaac agatgtcgaa ctagagtctg
    52261 cttggactga tttgtattta ttcgaagagc gctattacct agtcgttcac ttctggatgg
    52321 aaaatctcaa ccaagcggat gtagagaatc agattgctcg aattttggag tttgctgaaa
    52381 agagcgatcg tacaccagaa acattgaatg aatatggaca atgtctgatg gcgcgtaatg
    52441 ctatcgagcg aacgaaattt tattttgtat aaatcagcat tatagtaaga gactgtgaca
    52501 aaaattgtgt cgcagtcttt ttttcgtata aatgtgttca aaagcttgca tctgcggtaa
    52561 caagcccaac caaacgaaaa atattgggaa ctatttttgt ttagctgttc ttattacttg
    52621 atgcagaata gctttttcac gccctctttt ttcgtgtaaa taaattctta gacgtaaata
    52681 agttcagagg gagaaaaatg ctgcttgcaa tgaatgaata taataagtgc tgttttgcta
    52741 aacatgcaga aaaaggaaag aaatattatt gtcgtggctg tcgaaaaaga gtgattttga
    52801 aaaagggaaa gaaaaaatgt gctcattttg cccatcaaaa atcagataac tgttcggtct
    52861 ttagtgaaag agaatcaaaa gagcacttac agttaaagga atgcttcatg gattggctgg
    52921 gacagtcagt agaaccagta tttttggaag cctatttgcc gaggttgcgg caaaggccgg
    52981 atattttgtt ggcgaatcta gcaatagaaa tccaatgttc tagactgtct caccagagat
    53041 ttattgaaag aacccaaaat tatttgaata acagttacca agtatggtgg atacttggta
    53101 attcttttct tggacaatcg caattctcat tgatagaaaa aagttgctgc tactataata
    53161 gaaaaagagg agtgcattgc tggaaagctg atctcaagaa acaaaagctt tatttgtatc
    53221 atcatattac agagactgta tcagggcata tttccttttg cagtagctgc tggacttttt
    53281 ctagtaggga ccttaaagaa atattcacta gtaacgaaat taaaatgaac caaatgaaaa
    53341 aaatagaaaa agtgagtgaa gatgggaaga aatggctgac acgccaactt attcacaagc
    53401 aaaagaatac agtatccata caagaacaat gctacttgcg tcataaacat cttttgcatc
    53461 tatcatcgtg gatctatcaa aaaagtcggt ttttctttta tttacaagaa caggtttttc
    53521 tttatcgaat gttgtacgaa gaagccttgg aaaaccagaa agttcctgat ttcaactcat
    53581 ggctttgcca aataaaagaa cataaaaggg aatggctttt tcctatgatt gatgaagaga
    53641 tggtctatca gcagtttttt aacgaatgca tacacttgtc ttctttgaaa aaatagtaag
    53701 tataaaaata ggctaggaat cggttttcta tgtaaaacaa gatttagtta tgctaaaatt
    53761 gatggaagca gacacatgaa tgagtagaaa agttagatgc tgtgacatgt ggatgttaga
    53821 ctatgatcta ttttgataga tagagctaag ggaagaaaag gagtggattt catggaagta
    53881 aagcagttgc caaaacgaga agaattgcct gaaaatttaa cttgggactt gaccaagatc
    53941 ttttcaagcg accaagagtt tgatgagaaa tatttggaat tatcagaaga gttaaaacaa
    54001 tctgaaaaac acaaaggaac acttgatcaa ggcgcttctc aatttttaaa tgcgattgaa
    54061 ttcgtattga gggtttatcg ccaaactgaa gtcatttatg tatatgcgca ccttaaaaac
    54121 gatcaagaca ctggaaatac agattaccaa gcgctttatg caagagcaag cagtctgttt
    54181 tcgaaagtta gtgaagccgt ttcctggttt gaaccagaaa tattgcaatt gtcagatgac
    54241 cagatttggc aatatttcaa agaagaacca aaattggaag tctatcgcca ttatatccag
    54301 caaatagtag ataatcgagc ccatgtctta tctgctgagc aggaatctct tcttgctgga
    54361 gcaggtgaaa tctttgatgc ttcaagtgat acatttgctg ttttgaataa tgcagatcta
    54421 gtttttccaa cgattgaagg agaaaatggt gaaatagtcc aattatctca tggcgtgtat
    54481 ggtcagttgc tagaaagcac ggatcgaagg gtgcgcgaag cagcatttaa ggggttgtac
    54541 agtgtttacg aacaatttag aaatacattt gcttctactt taggcacaca tataaaagga
    54601 cataatttta aagcgaaagt ccgtaattac agctctgcca gagaagcgtc tttgagcaat
    54661 aatcatattc ctgaaagtgt atacgatact ttggtagacg tggtaaacaa gcatttgcct
    54721 ttgttacatc gatacatgga attacggaaa cgtttattag aagtggaaaa actgcacatg
    54781 tatgatcttt atacaccggt cttaggggaa gctccaatta cctttacgta cgaagaagca
    54841 aaagaaaaag ctttagaagc actgaaacca atgggtgaag aatacatggc catcgtagaa
    54901 aaagcattct ctgaacgttg gatcgatgtt gtcgaaaata aagggaaacg aagcggtgct
    54961 tattcttcgg gaagctatga cacaaatcca tatattttat tgaattggca tgatacgctg
    55021 gatcagctat ttacgcttgt ccacgaaatg ggacatagtg ttcatagtta tttcactcgt
    55081 tcgaaccagc cttatgtgta cggcgactac tccatctttt tagcagaaat tgcttcgaca
    55141 acgaatgaaa atatcctaac ggagtattta ttggaaacag aaaaagaccc tcgtgtacgg
    55201 gcttatgtac tcaaccatta cttggatggg tttaaaggaa cagttttccg ccagacacaa
    55261 ttcgctgaat tcgagcattt catgcatacg gaagatgaaa aaggtgtgcc attgactagt
    55321 gaatacctaa gtgatagtta tggtaaattg aatgcaaaat attatggtcc agcagtcgaa
    55381 gaagacccag aaattaaatt cgaatggtca cggataccgc atttttatta taattactat
    55441 gttttccaat actcgactgg cttttctgct gcttcagcac tggcgaagaa aatactaaac
    55501 caggaaccag aggcactaga gaactacttg gcttacctaa aagcaggtaa cagcgattat
    55561 cctgtggaag taatgaaaaa agcaggagtt gatatgacac aggctgcata tattgaagat
    55621 gcaatgtcga tgtttgaaca acgtctaaat gaattagaag aattgatcga tcgtttataa
    55681 ggctttctat ttttgatttt ctcgaataga aacgaaaaaa atacgtcggc tctcttgtaa
    55741 gggagtcgac gtatttttt
//
LOCUS       DACDNO010000013        55425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDNO010000013 DACDNO010000000
VERSION     DACDNO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.013"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            296..1426
                     /locus_tag="IWI22_000804"
     CDS             296..1426
                     /locus_tag="IWI22_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9168214.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1518..2273
                     /locus_tag="IWI22_000805"
     CDS             1518..2273
                     /locus_tag="IWI22_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9168215.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2331..4370
                     /locus_tag="IWI22_000806"
     CDS             2331..4370
                     /locus_tag="IWI22_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9168216.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4479..5630)
                     /locus_tag="IWI22_000807"
     CDS             complement(4479..5630)
                     /locus_tag="IWI22_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9168217.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5784..6314)
                     /locus_tag="IWI22_000808"
     CDS             complement(5784..6314)
                     /locus_tag="IWI22_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168218.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6413..6763)
                     /locus_tag="IWI22_000809"
     CDS             complement(6413..6763)
                     /locus_tag="IWI22_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9168219.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(6920..8449)
                     /gene="ahpF"
                     /locus_tag="IWI22_000810"
     CDS             complement(6920..8449)
                     /gene="ahpF"
                     /locus_tag="IWI22_000810"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9168220.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8459..9022)
                     /gene="ahpC"
                     /locus_tag="IWI22_000811"
     CDS             complement(8459..9022)
                     /gene="ahpC"
                     /locus_tag="IWI22_000811"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9168221.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9166..9828)
                     /locus_tag="IWI22_000812"
     CDS             complement(9166..9828)
                     /locus_tag="IWI22_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9168222.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10012..11658
                     /locus_tag="IWI22_000813"
     CDS             10012..11658
                     /locus_tag="IWI22_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9168223.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11649..12731
                     /gene="uxuA"
                     /locus_tag="IWI22_000814"
     CDS             11649..12731
                     /gene="uxuA"
                     /locus_tag="IWI22_000814"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9168224.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(12839..14392)
                     /locus_tag="IWI22_000815"
                     /pseudo
     CDS             complement(12839..14392)
                     /locus_tag="IWI22_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            14540..15681
                     /locus_tag="IWI22_000816"
                     /pseudo
     CDS             14540..15681
                     /locus_tag="IWI22_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(15807..16886)
                     /locus_tag="IWI22_000817"
     CDS             complement(15807..16886)
                     /locus_tag="IWI22_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9168225.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(16989..17429)
                     /locus_tag="IWI22_000818"
     CDS             complement(16989..17429)
                     /locus_tag="IWI22_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9168226.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(17486..17896)
                     /locus_tag="IWI22_000819"
     CDS             complement(17486..17896)
                     /locus_tag="IWI22_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9168227.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(18076..18510)
                     /locus_tag="IWI22_000820"
     CDS             complement(18076..18510)
                     /locus_tag="IWI22_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9168228.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(18610..19458)
                     /locus_tag="IWI22_000821"
     CDS             complement(18610..19458)
                     /locus_tag="IWI22_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9168229.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            19847..21613
                     /locus_tag="IWI22_000822"
     CDS             19847..21613
                     /locus_tag="IWI22_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9168230.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            21715..22974
                     /locus_tag="IWI22_000823"
     CDS             21715..22974
                     /locus_tag="IWI22_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9168231.1"
                     /translation="MKKRSVKQLGLGLISIGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            23121..24422
                     /locus_tag="IWI22_000824"
     CDS             23121..24422
                     /locus_tag="IWI22_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9168232.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            24425..25276
                     /locus_tag="IWI22_000825"
     CDS             24425..25276
                     /locus_tag="IWI22_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9168233.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            25289..26104
                     /locus_tag="IWI22_000826"
     CDS             25289..26104
                     /locus_tag="IWI22_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9168234.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            26255..27421
                     /locus_tag="IWI22_000827"
     CDS             26255..27421
                     /locus_tag="IWI22_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9168235.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            27651..28298
                     /locus_tag="IWI22_000828"
     CDS             27651..28298
                     /locus_tag="IWI22_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9168236.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            28382..29326
                     /locus_tag="IWI22_000829"
     CDS             28382..29326
                     /locus_tag="IWI22_000829"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9168237.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            29461..30912
                     /locus_tag="IWI22_000830"
     CDS             29461..30912
                     /locus_tag="IWI22_000830"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9168238.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            30933..31769
                     /locus_tag="IWI22_000831"
     CDS             30933..31769
                     /locus_tag="IWI22_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9168239.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            31769..32428
                     /locus_tag="IWI22_000832"
     CDS             31769..32428
                     /locus_tag="IWI22_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9168240.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            32554..33243
                     /locus_tag="IWI22_000833"
     CDS             32554..33243
                     /locus_tag="IWI22_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168241.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            33249..34205
                     /locus_tag="IWI22_000834"
     CDS             33249..34205
                     /locus_tag="IWI22_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9168242.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            34249..34761
                     /locus_tag="IWI22_000835"
     CDS             34249..34761
                     /locus_tag="IWI22_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9168243.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(35017..35565)
                     /locus_tag="IWI22_000836"
     CDS             complement(35017..35565)
                     /locus_tag="IWI22_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9168244.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(35654..36835)
                     /locus_tag="IWI22_000837"
     CDS             complement(35654..36835)
                     /locus_tag="IWI22_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9168245.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(37071..38579)
                     /locus_tag="IWI22_000838"
     CDS             complement(37071..38579)
                     /locus_tag="IWI22_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9168246.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            38738..38929
                     /locus_tag="IWI22_000839"
     CDS             38738..38929
                     /locus_tag="IWI22_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168247.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            39132..40049
                     /locus_tag="IWI22_000840"
     CDS             39132..40049
                     /locus_tag="IWI22_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9168248.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            40042..41115
                     /locus_tag="IWI22_000841"
     CDS             40042..41115
                     /locus_tag="IWI22_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168249.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            41263..41781
                     /locus_tag="IWI22_000842"
     CDS             41263..41781
                     /locus_tag="IWI22_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168250.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            41762..42154
                     /locus_tag="IWI22_000843"
     CDS             41762..42154
                     /locus_tag="IWI22_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9168251.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            42230..43165
                     /locus_tag="IWI22_000844"
     CDS             42230..43165
                     /locus_tag="IWI22_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9168252.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            43298..44077
                     /locus_tag="IWI22_000845"
     CDS             43298..44077
                     /locus_tag="IWI22_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9168253.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            44070..44387
                     /locus_tag="IWI22_000846"
     CDS             44070..44387
                     /locus_tag="IWI22_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9168254.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            44631..46241
                     /locus_tag="IWI22_000847"
     CDS             44631..46241
                     /locus_tag="IWI22_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9168255.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            46398..47246
                     /locus_tag="IWI22_000848"
     CDS             46398..47246
                     /locus_tag="IWI22_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9168256.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            47477..47956
                     /locus_tag="IWI22_000849"
     CDS             47477..47956
                     /locus_tag="IWI22_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168257.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            47989..50217
                     /locus_tag="IWI22_000850"
     CDS             47989..50217
                     /locus_tag="IWI22_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9168258.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            50346..51989
                     /locus_tag="IWI22_000851"
     CDS             50346..51989
                     /locus_tag="IWI22_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9168259.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(52062..52607)
                     /locus_tag="IWI22_000852"
     CDS             complement(52062..52607)
                     /locus_tag="IWI22_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9168260.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(52630..53115)
                     /locus_tag="IWI22_000853"
     CDS             complement(52630..53115)
                     /locus_tag="IWI22_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9168261.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(53105..53917)
                     /locus_tag="IWI22_000854"
     CDS             complement(53105..53917)
                     /locus_tag="IWI22_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9168262.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(54056..55168)
                     /locus_tag="IWI22_000855"
                     /pseudo
     CDS             complement(54056..55168)
                     /locus_tag="IWI22_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(55189..>55425)
                     /locus_tag="IWI22_000856"
     CDS             complement(55189..>55425)
                     /locus_tag="IWI22_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9168263.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT        18346 a        10054 c        10729 g        16296 t
ORIGIN      
        1 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
       61 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
      121 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
      181 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
      241 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggattttggg
      301 tccttatttg actttgtcca tgatcggtct tttagaagta tacagtgcca gttcttatcg
      361 tttattgcaa gccgatgaaa atacaaaaag tctattattg cgtcaactaa ttttcatatt
      421 tttgagttgg agcgtgatct tcttagctcg ttcagtcaaa ctacactatt tacttcaccc
      481 taaaatagca ggatacggtt tagccttatc gattttcttt ttagtattag taagaatagg
      541 gatattcggt gtcactgtca acggcgcaca acgttggatc tctctgtttg gcattcaatt
      601 ccagccttct gaactggcaa atcttttttt gattttttat ttaagctggt tttttcgtga
      661 cggaaatagt agcccaaaag atctaaaaaa accattcctg attacagtag gtataacttt
      721 tctgatttta tttcagccaa agattgctgg agcattgatg atcctttcga ttgcgtgggt
      781 catattttgg gcagcggcgg ttccatttaa aaaagggatc tatctaatcg ttactttttc
      841 tgcattgctg attggagcag caggcggggt attatattta ggaaataaag gttggcttcc
      901 acagatgttt aatcatgctt atgaaaggat cgcgacttta agagattctt ttatcgatag
      961 tcatggtgcg ggctatcaaa tgacccattc gttttatgca ctatataatg gtggcatatt
     1021 tgggcgggga ttaggaaata gtatcacaaa aaaagggtat cttccagaga cagaaacaga
     1081 ttttattttt tcgattatta cagaagagct tgggctaatc ggtgcattgt gtgtgctttt
     1141 cttattgttc tcattatgca tgagaatctt ttgtttaagc agtcgctgta aaaatcagca
     1201 agctggattg tttcttttag gattcggaac tttgctattc gttcagacga taatgaacgt
     1261 gggaagtatc gcggggctga tgccaatgac tggtgtgcca ttgccatttg ttagctacgg
     1321 aggaacaagt tatctgatcc tttctttagg aatcggaatc acgttgaata tttcttcaaa
     1381 gatacaggcc gaagagttgg cgctctatcg acctgaaaaa caataaagag aaactgagaa
     1441 actctcagtt tgaattatca taaagtacaa tcttttattt ttcaagcgag aggttctttt
     1501 agaaagagga attcgcaatg aaagtatctc aaaaaatcat attatctatt ttgttactgc
     1561 ttacattagt agttggctat ttttcgattg agtttatcca tggattttcg tctgcaaaac
     1621 agagttcaac tgtgaaaaag gtagagccga aaagtatccc taccacacta aatgtggctt
     1681 tgattggttc agatgctcgt tcgaaagacg aaaacggtcg ctcggattca cttctggttg
     1741 cacaatacaa ccagaaaaca caacaagcaa aactgatctc tatcatgaga gattcatatg
     1801 tcgatattcc aggttacgga atgaataaga taaatgcagc ttactcttat gggggagttg
     1861 atttattaaa tcaaacatta aaggaaaatt tcaaatttga agctccctat tatgcaagta
     1921 tcacatttca agattttatc gattgcgtta atgaactgtt tcctggtgga gtcaagatcg
     1981 atgcagaaaa atctctagat ttagatggcg tatatataaa caaaggagag caagtgatgg
     2041 atggtaatac tttactccag tatgctcgat ttcgggaaga caaagaagga gacttcggga
     2101 ggatcagaag gcagcagcaa gtgatcaaag ctatctctca gcagctgaaa gacgtaactt
     2161 caatattcaa gctacccaaa gcggttggga aattacttgg tagtatccaa acgaaccttc
     2221 cagaaagtgt gcttctggat tgtggtatgg atttcctcaa agacatatgt tagaataaac
     2281 aggtataaat agtgaaataa aggaatgaca agcaagagaa ggaggaaaaa atgaaaagaa
     2341 gtgacaagca cggcaaaaat cgaacaggcg cttatattgc cggcgcagtg atcttaatag
     2401 cagctgcggg tggcggttat ttttactacc agcactatca agaaacccaa gcagtagaag
     2461 ctggagaaaa gacagttgag caatttgtcc aagctttgaa caagggagat tataacaaag
     2521 ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc attatctgaa aaagagatct
     2581 tagataaata ccagaatata tacggtgctg ctgatgtcaa aggacttcag atatcgaatc
     2641 taaaagtaga taaaaaagac gattctactt acagtttttc atataaagca aagatgaata
     2701 cctcattagg tgaattgaaa gatctttctt ataaaggaac attagacaga aatgatggtc
     2761 aaaccacgat caactggcaa cccaacttgg tttttccaga aatggaagga aatgacaaag
     2821 taagcctgac cacacaagaa gcagcaagag ggaatattat agatcgaaat ggtgaaccat
     2881 tagcaacaac aggcaaacta aaacaattag gagtcgttcc aagtaaactt ggagatggag
     2941 gcgaaaaaac agccaatatc aaagcgattg cttcctcctt cgacttaaca gaagatgcta
     3001 tcaatcaggc gatttcacaa agctgggtac aacccgatta ctttgtccca ttgaaaatca
     3061 ttgatggagc aacgccagaa cttccagctg gagctaccat tcaagaagta gacggcagat
     3121 attatccttt gggtgaagca gctgctcagc tgattggtta cgtgggagat atcacagcag
     3181 aagatattga taagaatcca gaattaagca gtaatggaaa aatcggtcgc tcaggtttgg
     3241 agatggcttt tgataaggat cttcgtggga caacaggtgg gaaattaagc attacagatg
     3301 cagacggtgt agagaaaaaa gttctgatcg aacatgaagt tcaaaatggc aaagatatca
     3361 aattgacaat cgatgcgaag gcacaaaaaa cagcttttga cagtctagga ggaaaagctg
     3421 gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct tgcgcttgct agctctccaa
     3481 gctatgatcc aaacaaaatg acaaacggga tctcacaaga agattacaaa gcttatgaag
     3541 aaaatcctga acatccattc atcagccgat ttgcgacagg ttatgctcct ggatctacgt
     3601 ttaaaatgat tacagcagca atcggtctcg acaacggcac tatcgatcca aatgaagtgt
     3661 tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg gggatcttat caagcaacgc
     3721 gtgtcagtag tgatgtatca caagtagact taaaaactgc tttgatctat tccgataata
     3781 tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa atttcgtaca ggcttagata
     3841 aattcatttt tggtgaagac cttgatttgc caattagtat gaatccagca caaatttcta
     3901 atgaagatag ctttaactca gatatcttgc tagctgatac tggatatgga cagggcgaac
     3961 ttctaattac tcctatccag caagcagcaa tgtattctgt ttttaccaac aatggcacac
     4021 ttgtctatcc taaattgatt gcagataaag agacaaaaga taagaagaat gtcatcagcg
     4081 aaacagcagt acaaacgatc gtgccagatc tgagagaagt tgtgcaagat gtaaatggta
     4141 cagcacattc tctttctgct ttagggattc cgttggcagc gaaaactggt acagcggaaa
     4201 tcaaagaaaa acaggatgta aaggggcaag agaacagttt cttgtttgct ttcaatcttg
     4261 ataaccaagg ttatatgatg gtcagcatgt tggaaaataa agaagatgat gattcagcaa
     4321 ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa ttatcaataa aaaattgctt
     4381 tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt ccttttttca aataaacggt
     4441 tttcacgatg tctctttcac aacttctttt tgatttactc acacttcata aacttttttt
     4501 gtaaaatatt tgagaacaag tggagaaata agtgtactta ataaaatcac gataactaaa
     4561 ggtgaatagt actgtggttc gatcaattga ctttgctgtc caatttgtaa aatgatcaaa
     4621 gccatttctc cacgcgaaat cataccagca ccgaccatca atgaactatg ttgagaaaaa
     4681 ccggcaatct gagcaccaaa ataacctcca agcaatttgg tcaaaatcgc tacaagcgtc
     4741 aatatagaaa taaagagtag ttgttcacta aagtcagaaa aatcaacttc taatcctaca
     4801 ctgacaaaaa atactggtat aaatactgca tatcctaaag cctctacatt gtggtatact
     4861 tcttctttaa cctttgtctg actgacagct atcccagcaa aaaaagcacc aatgacagag
     4921 ctaagcccaa caagatccgc tacgtaagac attcctaaac aaataactag cgatacgatg
     4981 ataatagaag aatttgcgta cactttttct gcaaatgaaa gtaggaaagg cgcaatccat
     5041 ttgactaata agaaaataaa aacaaaataa attagctgtt caatcaaaac aagcggtaaa
     5101 gaagcattgc tagaagcttc ccccgttaga aaagataggc tgaaacttag aactaaaacg
     5161 actaaaatgt catctaccac agaagcaccc aaaattgttg atccttcttt tgtattcact
     5221 acgtttagtt cttttaatac ttctactgaa atactgacag aagttgccgc aagaataatc
     5281 ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt tgcctgtcaa ccagccaaaa
     5341 acaaccggga agatgatccc aagcaaagca acgaacatac caggtcggaa atattttttc
     5401 aaaagagata aatcactttc cagaccagct aaaaacatta ataagatcac accaatctcc
     5461 gaaaagtcat ggactagaat atcaggtttg acccaattca atccagcttg gcctagcaaa
     5521 acaccaacaa gtaattgtcc aatcactgct gggataccta atctccgcga gatatgtgag
     5581 ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa taaattccat tctcatcatt
     5641 cctttgtata aaatatagcc cgaacttagc ataacataca ttattgaaaa aataggaaaa
     5701 tatactcaca aaaaaaccaa caatccaaaa gatttttttg gattgttggt ttttatacgg
     5761 tctattttaa taactaaaaa tgattaacgt atagtcattt ttcttattct gatgaaattg
     5821 ataatctcct ggatgcccat gaaaatcaaa ataaagccga atatttggaa tagaagcatc
     5881 aaacttttga atggattgaa caataagacg aatccgccaa tcaacaacag aatactacta
     5941 taaacactcc aacttttagc ttttgtattc atgctgaaga acaactggaa caagctgttg
     6001 atgataacag ctaatccaag aagaatcggc aggatagaaa cgattgcagg tgcaaaagca
     6061 tagacgatca gagctaagat caggaaaaaa agaccactga acagaccgat actccaagaa
     6121 cctgttttcc ttttaaaacg gtaagcctca aaaagattca acaacccaag caaaacaaga
     6181 taggcggtga tcagataacc gataaaatga aaaacagcag aagggtttag cacaatcgca
     6241 atcccagaaa taatataaac agcagctctt aataaggcgt atttttgaat attttgttta
     6301 atcgtctcaa acatataaga aaacctcctt tgacttattt taacgtgaac tagtcatttt
     6361 accaaattaa atgttacaaa atgagcgaag gagaagaata tctctggaaa aatcaacttc
     6421 tgaggatctt ttccattttt ttgccttttg ctagctcatc gatcatttta tctaaataac
     6481 gaatttcttt catcaagtta tcctcgatgt cttctactcg tacaccacaa acaacacctg
     6541 taatcaagga acgtgaagga ttggggacag gggcttccgc aaaaaattgt tcgaaagtca
     6601 cttgtttttg tagttgatat tctagttctg tttgggaata acccgtcagc caacaaatga
     6661 tttcatctac ttcctctttt atacgatctt ttctctccgc ttttgctaca tacataggat
     6721 aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc cataatcagt cacctttctt
     6781 tttttcaaat tatttactac ttatagtata taagaaaaag agaagaaaaa gcgattattc
     6841 aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat ccttttttcc gctctctgac
     6901 tttttacaat cttcatagct caagtgcgca tcagataatc gaatgctcct aaagctgcag
     6961 tagctcctga acccattgaa acaatgattt gtttgtatgg actatctgta caatcaccag
     7021 ctgcgaaaac gccggggata ttcgttgcgc catttttctc ggtcactatt tcatttcttg
     7081 gattcatttc gatcgttcca tctagccaat ccgtattagg gagtaagccg actagaatga
     7141 atactcctga aacagctagt gtatgcatct ccatcgtacg gcgatcgata taggagagag
     7201 attctacctg attcgtaccg ctgatttctt tcgtcgctgc atgtttgata acagtgacat
     7261 tcgcaagaga gactagttta tcctgtaaga tttgatctgc ttttaattct ggtaagaatt
     7321 ctagaacata tacatgtttt gctaacccag ccaaatcgat tgctgcttca acaccagaat
     7381 taccaccacc gataacagca atctcttttc ctttaaataa agggccgtca cagtgtgggc
     7441 aataagcaat tcctttattt ttaaactctt tttcgcctgg tacattgata gaacgccagt
     7501 gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga tataccgtta gctaagccaa
     7561 tattaataaa atgttgctct ttatttaagg aaacggcctg ttgattcatc ataatgtcga
     7621 tagggtaact acgcacatga ctttctactt gctgcatcaa ttttggacct tctgtgtaag
     7681 aggtaccaat catattttca atccctagtg tttcaacgac ttgtccgccg aaagaatcag
     7741 cgacaagtcc tgtacggatt cctttacgtg ctgcatagat agctgcacta ctagctgctg
     7801 gtccaccgcc aatcactaat acatcataag gttctttttc aaacaactct tcttcaatca
     7861 aagggccagt gattttttca acaatttgtt cgatcgtcat gcgtccgcta ctaaattctt
     7921 ctccatttaa aaacacagtt ggcactgcca tgattttttt atcatcgatt tctttttgga
     7981 acatcccacc ttcaatcatc gtatgagtaa tatttggatt taatacagct aatatattca
     8041 atgcctgtac aacgtcgggg caattatgac aagtcaagct aacataactg tcaaaatgca
     8101 atgtttgtgt tatttttttg attttaagac tcaaagattc actgattttc ggtgcccgtc
     8161 cactaatttg taacatagcc agtaaaaaag aggaaaattc atgaccgagg gggataccag
     8221 caaaaacgat tccactttct ttgccaactg gattgataga aaaactaggt gtgcgcatcg
     8281 aatctctttt ttcccaagag attttaggag acatttctgc tatttccttc gtaaattcag
     8341 ctagtttttg actattttca ctattatcca aactaacaga aaaaacaata ggagattcaa
     8401 tcagtgctaa atattgttcg agattttgtt tagtttcttt gtctaacata tccaccacct
     8461 aaatctttcc aacaagatca aaacttggtt tcaatgtttc gccactttct ttccattttg
     8521 ccggacaaac ttcacctggg tgggtacgta catattgagc agcgcgaatc ttatcaatca
     8581 gtgcactagc gtcacgaccg attccatctg cattgatttc catagcttga acgattccgt
     8641 ctggatcaat aataaatgtt cctcgctgag ctaatccttg ctcttcatcc aaaacatcaa
     8701 aaatacgaga aatctgatga gacggatcac cgatcatcac gtattcgatt ttaccgatag
     8761 catctgatgt atcatgccat gctttgtgag taaagtgggt atcggtagag caagaaaaga
     8821 cttcaacacc taaagatttc aatgtactgt attgttcttg taagtcttct aattcagtag
     8881 gacaaacaaa agtaaaatca gctggataga agcaaagaat actccagttc cccataagat
     8941 ctttttcact gacttctaaa aattctcctt gatgataagc atgtgcatta aattcttcga
     9001 tttttttccc aatcaatgac atagtaaaac ctccaataat aattatttat ttataattat
     9061 tgtaatttag ttggagttgc aaagtcaatg gatattttgt tatttaataa agattactca
     9121 aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga aaatgctact gctggaaata
     9181 ctcgggataa cgttgagtaa cagtttcctg ttctttcagc atcatgtgta agtgcaatcc
     9241 cgtttgaaat cgagcttctt ctggtttttt atcaactaaa gccttgagta aatcataatg
     9301 ttgttccatg atcgtttccc atggaagccc ctgaatggtc actcgtaacc agcggaaacg
     9361 ttctaaatgg gtattatggt catctaacca gttccaaact tcttttcttc ctgcaatttc
     9421 gaaacaagct tcatgaaata aattatctgc acggaaaaaa gcgcgttcgt catttatttt
     9481 gactgctttt tcttgttctt ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa
     9541 tttggaacaa cattcaatca tgatttgctc ttccaactgt tctcggacaa agcgagcatt
     9601 ttctgcagaa acaagattaa ttttggaaac atacgtacca ctttgaggta tcgtatacac
     9661 gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag
     9721 atgttcctcc agtgcctttt cagatatttt ccttccaggt tctaagtcag catagataat
     9781 ttgtttacga atcgcttgat aagcttgtga ttgcaagctg cttttcatct ttattcctcc
     9841 aaaatcattt ctatgaacgt agtataacag aagaggtctg aaaaacaatc tggtatgtca
     9901 cattaactag taaatttgta taaatagtaa aattttggtt gacatagcga tttttattgc
     9961 ataaaattca cttgaataca aaaactaata tgttagttta ggaggggaaa aatgattgat
    10021 ttaaacaact ggaggaactt tatccctgaa ttatctcaaa aagaagtaga aatacctcat
    10081 tttgaccgtg catcattaag agaaaatgga caaaaaaatc ctgtctggat ccatttcggt
    10141 ggcggaaatc tttatcgagg atttcatgca gaaatcgctc aacgtttagc ggatctaggc
    10201 gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg aagaaataat tgaaagggct
    10261 taccaagatt ttgatgattt gattcttgaa gtggtcatgc caagcagtgg agagttcgtc
    10321 aaacgtatat tagctgcgac agctgacagt ctgtattgtc atccagattg ttctgaacac
    10381 tttgataaga tgattcgaca ctttgaaaat cctagtttac aattagttac ttttgctatt
    10441 actgaaaaag gttatcgatt gaaagataca aataataaat atataggacc gattgcagtc
    10501 gatataaaaa atggtccaaa gaagccatca catacaatga gtattcttgc tgtgctactt
    10561 tataaacgtt ttttatctgg agcttggccg attgcacttg tctctacaga taatttttca
    10621 caaaacggta gtcaattacg tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa
    10681 ggttatgtac aaaatacatt tatccaatac ttatcgaatg aagaaattgt tgcgtttcca
    10741 tggacgatga ttgatcgtat caccccgaat ccttccttgt cagttagcaa aatgttggca
    10801 aaagagggct tttcaaatat gtcgattttc caaaccgcaa aaggaaataa tattgctcca
    10861 tttgtcaata cagaggacat ccattattta atcatggaag atcattttcc caatggacgt
    10921 ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg caacggtaga taaagcagat
    10981 acgatgaagg tgacgacttg ccttaatcct ttgcatacgt ctttagctat tatgggttgt
    11041 ttactggggt ataggagcat agctgaggag atgaaagatc cagatttatc gctgctagtt
    11101 tctgaaatcg ggtacaatga aggcctccca gttgtggagt ctccaggtat cattgatcca
    11161 aagatctttc tggaagaagt aattactgtg cgcttttcta atccaaatat tcctaatatg
    11221 cctcagcgaa tcgccacgga tacctctcaa aaggtggcga ttcgttatgg tgagacgatt
    11281 aaaaaatata tggaaataga agttaaagac ccgcagacac ttgtattcat accattgact
    11341 ctcgctgctt ggcttcgata ccttattggc attgatgatc aaggaaaacc attcatcccg
    11401 agtccagacc cactattgac agaccttcag cataagcttt ccgatattca actgggtacg
    11461 caagatacat cacatattca tgagaaaatg gctcctattt tgtcaaataa agaaattttt
    11521 ggtgtagatc tttaccaagc agagcttggc agaaaaatag aagaaatgtt catcaagatg
    11581 gtagcaggac caaatgccgt acgaaaaaca ttacaggatt acttacaaga acatggagga
    11641 aatttatcat ggaaatgact tttagatggt acggatcaca ggaagaaaag attaccttag
    11701 atgaaatcag acagattcct gcggtcaaag ggattgtagg cacattgatg gatataccag
    11761 taggagaggt atggcctagg gagaggatca aagaactaaa agaagaaatt gagtctcgtg
    11821 ggttaacact aaaagtaatt gaaagcgtca atgtgcatga tgccattaaa attggattac
    11881 cttcccgtga gcagtatatc gaaaactata agcagacaat ccgaaacttg gcagaatatg
    11941 gaatagaagt catctgctat aattttatgc ctgtatttga ctgggtgaaa tctgaccttg
    12001 actaccgctt agaagacggc tcatccacgt tagcttttat atctgatgat attccagatg
    12061 atccaagaga aatcgtcgcg cgcatcgaac attccagcaa tgaatttgga ttgccgggat
    12121 gggaacctga gcgcttagct cctatcaaat ccttgcttga agcctatgca tcagtagacg
    12181 aagaaaaact tcgagaaaat tttgcctatt ttctccaatc aatcatccca acatgtgaag
    12241 aggtaggggt caaaatggct gtacatccag atgatccgcc atactcgatt tttggattag
    12301 ctcgaatcgt aaagaatcga gaagatttag attggttgtg tcgcatagta gacagccctt
    12361 ataacggaat cactctttgt acagggagta ttgcagaaga tccagaaaat gatgtatatg
    12421 ggattttagc agaattttgc caaagagatc gaatcccatt tgcacatgtt cgaaatatta
    12481 aatttttagc taatcgagat ttctatgagg caccacataa atccgaatac ggttcattag
    12541 acatgtaccg catactaaaa acaatgtatg acaacggatt taacggatac atccgtcctg
    12601 atcatggaag aatgatctgg aatgagacag gaagaccagg atacggctta tatgatcgag
    12661 cattaggtgc tgtttatcta aatggattat gggaagcaat cgaaaaagga aacaaaagta
    12721 aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa taagaaggat gttcaaattc
    12781 ttgatacata aatccggagg ttgttaataa gcattcggat ttatgtatct tatttggtct
    12841 aggaagtaac ttgattttct tttttcgatt gatccatgag tgcttcttct tttaattcaa
    12901 agatatattc tgagatcctc ataatcactt ttttccagtc ttctggtgtg atgattcctt
    12961 ttaaaatata gatttttttg atatgagaag gcaattgttt caaagggcag tcgctgataa
    13021 cgatgttgca gttctccata aaatcatttt gtattttgat cgattcattt gaaaaaattt
    13081 gttctatctt aacgatcaga tctttactga tataaaaatc acgcgtatag ttcacaccaa
    13141 tcacaattcg cggctgtttt ttgtagcgat catacaaatg tactaggtct tcaaccatcc
    13201 attgcacatt ttccttttct aaaataactg gtaacaagtg ttgattttgt tctctgaaat
    13261 ccatataaaa ttgttcaatg gcggtttgga cttctttcaa tacgctatgt tcttgataaa
    13321 gcatgaaagt aggaaggggc gtatttggtt gattgaagtt aagaaatcca ttataaagtt
    13381 tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa
    13441 atacagtaca aagggactcc accatttctc ttgaataaag aacatgagga acgtttgctt
    13501 tttttaaaag tttgtattgc tccaagcttg tttcttccga ttcgattttc gtgatagaaa
    13561 gtcgagctaa aatattcact aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca
    13621 aaatatctac agagagatca ataaaaaagt caatatattc atactctgtg aattcttttt
    13681 gtacgtcttt cacagaagtt gcaggaaaat tctctttaca tatttttaat agaaaatata
    13741 atttatctat cataccgcta tttagtcggt caagtacttc ttttttaaaa aaagtattca
    13801 tcataaaagc cactacttgc ggatcttcat cataaaattc tgtagcagta cttgaaaaga
    13861 tgttccaata aatatctaat gctgcatatt gaatttgtac ctcttttcca gaaaaagact
    13921 ttctgccatt attagaaaag gagatagaca aaccgtacta tgctaaaaat gcattaatct
    13981 ttttcatttt cgtatacaaa taagaggcac tatagtttgt ttgctgactg aaactgataa
    14041 tcgaatttgt ctgacagctg aacaaagaag tcaagatctg ataactgact gattcgttgc
    14101 agtatcttcc aaatagacga taatagcagt aagccggatc aattccttca aaattcaact
    14161 ttaaaaaagg tttttgctta tcaatatgca gttctgttag attgagacgc tcaaagtctt
    14221 gttctaattc tttgatgtat agtaaaaaat ttgattcaga catatggaaa tgatctgcta
    14281 aatattgtgt agtaataaag aaacgatgtt cagataggaa aagaaaaata tctaattttg
    14341 tattgctttt tttcttcaag ataaacgttt taatcgcttc ttgagtttgc atgaaagagg
    14401 ctccttttta cttaaattgt taaaacttga aaatattact gtttttttca taaatttaga
    14461 caaaaaaaca gtaaacatat aggtagaatc tctactatct tatttcttgc atacaaaaaa
    14521 atcaaggagt gagagagtta tgagatattc aaccatctta atatcaacat ttgcagcctt
    14581 aagcttatcg cttactataa atcagacagt ccacacagag gagacagaaa cagccaaatg
    14641 gacagaaacc aagtgggcca acgtcaatcg aattaccttt caggatgaat tttcatcctt
    14701 caattcagga gtcgcaaaat taagaaaagg acaaacagcg accatcaaag caaaaattga
    14761 ctattcggga gacttagcac ctatactaca atcagctgtt acatttgaga atgttgatcc
    14821 cactttgtcg attggctatg acgagaacac tttgactatc caacacaagc aagcgatatt
    14881 taccttcacc gttacattaa atcaggactt gttaaaacca gcgcttttta ctgtcaaggt
    14941 atcagatgga ctaccgaacg atcatcattt aacgccgtat agtcagcggc agacaattga
    15001 gcaagattca gccatcgact caggaggaga tcttgttgaa gaaccaacag ataaaccaga
    15061 aaatgaaaat aagccagaag taccgcctac agaaaatcca gacggagaac agaaaccgga
    15121 aatagaacct ggagaagaac ccgatacaga aactcaacca gagccagaca ataaatcaaa
    15181 acctgaaata acccctggag aaaaaccaga tgtaactcca gaaccagata cggattctgg
    15241 aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa
    15301 accagaggta gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag
    15361 agaccaagga ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga
    15421 gagtaaaaaa tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc
    15481 acgaatattt gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt
    15541 tgctgtcctg aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt
    15601 atactgtgga gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt
    15661 aaacaaaaca gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa
    15721 agaaatatcc agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc
    15781 atactttatc tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc
    15841 gggatctttt tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc
    15901 aagccccaaa cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc
    15961 ggcagttctg ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg
    16021 acaatcaaaa tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct
    16081 atgaaagata gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg
    16141 aaaatcatga tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta
    16201 gtgagcaacg tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta
    16261 ttgacaaact tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt
    16321 aaaaacaatc ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag
    16381 atgaatgaca tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca
    16441 atcgaagcgc cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata
    16501 tattgtaaaa caggatctgc atcttttggc tgatgctcgt aaaaattaat aacggactgg
    16561 atcatgtcaa atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata
    16621 aggtaaacga tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat
    16681 cgctgaatga tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg
    16741 gttgtaatca tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga
    16801 cggcgtattt tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa
    16861 taacaaaaaa taaacacgtt attcattata tgatggaata tgaaaataag aaagagcaaa
    16921 aagaaaaacc gctaaatgac gttatcttat gagttggttt tcctcataaa atcgttcatc
    16981 tagcagtttc agcccttttt ctggtcttga tgaaaattat ctagcatttt tcgggtcaga
    17041 tgttgcagtg tatccaattc ttcctgttca agtggaagat aagacttgat cttttgtgga
    17101 ataccttctg cttgtttttg caacgcagca ccttgagcag tcagcgtaac atttacgatt
    17161 ctttcatcac ccgtttcacg ctgacgatta atataatttt tttctgctaa cttttttagt
    17221 aagggagtca gcgtaccaga atccagacac aaatcatttc ctagctcttt cactgaagtc
    17281 tttttctttt cccataaaac taacatcaca agatactggg tataggtaag attcagcggc
    17341 ttgaggaggg gagtgtagtg tctaatgatt tctttagagg cggcatataa aggaaaacat
    17401 acttgattaa ctaaatatag atcttccatt tttccacctc cgctgtttta ctatagtgta
    17461 tcatagcgga tttacacaaa aaagtctatt cactaactgc tttcagagta acttctatat
    17521 ttccagctgt agcttttgaa tacggacaca ctttatgcgc tgcctctaat aattcttgcg
    17581 tttcttccag agaaagacct tcgatagatc cttcaatctc tacacctaag acaaagctcg
    17641 tttcactttc tgaataaagg gaaacttgtg cactgacagt agaagcccca gagatatttt
    17701 tttgctgttt gatcaattct aatgcactgt taaaacatgc actgaaacca gctgcaaaca
    17761 attgttcagg gttggttgca ttttcgacgc gtttaccagg agctgcaata ttcaattcga
    17821 atgtacgatc gggattatgc acttctccag agcgtccgcc tgtgttgatc attttcgtag
    17881 aatacatttt tttcataaaa ttaccacctt taattatatt ttatacaatt gaattttaca
    17941 caatataatt gtggaaagca aagaaatgga ttggcataag ataaataaga gaaagcacat
    18001 gaaacagacg cgctttctcg aatagcaaac atatacaaaa aatatcagag acaaaaaata
    18061 atctcatcat aaacattaca tatatttctc aaaaagttgt tttctttctt caaaaattac
    18121 ttcacgatgg acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt
    18181 agtcacacca aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa
    18241 tgctttatta tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg
    18301 atgttcagca gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct
    18361 cgtaccatac aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat
    18421 cagttgtatt tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa
    18481 tacaaaatcg atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag
    18541 ttactagatt cttttattat accagctaaa gaaaacgcat tcaaaaaaat agcggattta
    18601 cttaccaact cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct
    18661 gctgacaatg acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa
    18721 ttcatgatca gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt
    18781 tgcctctagc agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc
    18841 caaataatgt tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata
    18901 agtcatgaca attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc
    18961 aaaaataaac agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt
    19021 attcaagtgg aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga
    19081 gttgttttta tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga
    19141 caacaaccga tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaac
    19201 caagcttccg aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac
    19261 caacataacg atattcaaca ctaaaataac tatagctcgg tcaaagccta aagcagcttc
    19321 cagtaatatt cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat
    19381 attgatagcg actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa
    19441 ttgtctcgtt tctttcattt attcacttcc ttaattcatt aataatactc ttatcttatt
    19501 ataacttact tttcacctta agttaacttt aattaataaa aaaattaggc tagtctaaat
    19561 tattatgttt cattcttcta gaatatactt ctacgccaaa aactgttaaa aaacttttac
    19621 tttagaaaat tggagaaatt gatcgcttaa gataaattca ggacactaaa tagtacagga
    19681 agtagactaa aacttttaaa agttttaagt ctatctattt ggcaagttta aaaaataaaa
    19741 attcggtatc aaaaaactgt taccgataac atttttgaaa gcgttgtctt ttgcttctct
    19801 atcacttata gtgatgacgt catattaaaa cacaaggagg aaatcaatgg atacagctgc
    19861 aatttatcat cgaccggaaa gtgaatatgc atttctatat acgaatacaa gatttcgtat
    19921 tcggcttcgt actaggaaaa atgatatcaa aaaagtatat gtgctatgtg gagatccata
    19981 taccataaca actgaaaaat ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac
    20041 caatgttcat gattattggg aaattgaagt gacgagcgaa acaagtcgct tacagtatgc
    20101 gttccatgtt gttggggaag ataacacgga ctgtttttac ggtgatcaag gaatttttcc
    20161 ttatcgagaa aatgtgatta ctgaaccaaa tttttatttt cgaatacctt attttcatca
    20221 aatcgatcga ttcactattc cagaatgggt aaaagaaaca gtatggtatc aaatttttcc
    20281 cgaaagattt gcaaacgggg acaaagcaaa tgaccccgaa cacactttac cttggggaag
    20341 taaagatcca gaccgagaag actttttcgg gggagatttg caaggtgtga tggatcattt
    20401 ggattacttg acggaccttg gcatcaatgg catctatttt tgtcccatat ttaaagccac
    20461 atccaatcat aaatacgata cgattgatta ttatgaggtg gatcccgctt ttggcgataa
    20521 aaaactttta aaaaacttga ttgatgaagc acataaaaga gggatacgta tcatgttcga
    20581 tgctgtattc aatcatatgg gtgtccattc accacagtgg caagacgttc tcaaaaacgg
    20641 agaaaaatcg atctataaag actggttcca catccgtttt ttcccagtag attcttatca
    20701 gatgactgac ttaccagaaa ctgctgaaaa tatcccatat gatacttttg cttttactcc
    20761 attcatgccg aaactaaata cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc
    20821 aacttattgg ataaaagaat tcgatattga tggatggcgt cttgatgttg ctaatgaagt
    20881 cgatcatcat ttttggaaaa aattcagaga agcagttaca gaaatcaaac cagacatcta
    20941 tatccttgga gagatttggc attcctctca agcttggctg caaggggatg aattccatgc
    21001 agtgatgaat tatgcattta cagattcgat taaagattat tttgcaaaaa agaaaatcac
    21061 agcttcacaa atggtcagtg gaatgaatca tcagcaaatg ctttacagag atcaggtgaa
    21121 cgaaggaaca ttcaacttat tagattctca tgatactgcc aggatcttga ccctttgcca
    21181 aggtaacaaa gaattgatga agtctgtgat ggcctttatg tttcttcaaa aagggtctcc
    21241 ttgtatctat tacgggacag aaatagggat gactggtgaa gatgacccgg attgccgcaa
    21301 atgtatgata tgggagaagg aggaacaaga tctagagcta ttcggtttta taaaagaact
    21361 tgtttctcta aggaaacagt tgtctaagat tatttctgaa ggttctactc agtggctaat
    21421 agtaaatgat cgtgaagata agctctattt cacacggaaa ctagagggac agatcatcta
    21481 tgtttacttt aatcagtcaa aagaaccttg ggtagtagaa caggaaaatg aggtgattct
    21541 tagtcaaaat tgtcagttgt tagaagacgg gaaagcagag atacgacaat acggattttt
    21601 gatcatgtgt tgaaaactgt taccggtaac aacaagaaag cgtttgcaaa tgaagatttg
    21661 tgcccattat aataagggtg taacagcacg ttgaatacaa aggaggaaat gaagatgaaa
    21721 aaaagaagtg tgaaacagtt gggtttaggg ttaatctcta taggaatctt aggcggatta
    21781 gcggcttgtg gcaatggtaa ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta
    21841 acagtttccg ttgattctgg atataaagat tatataaacg aaataaaaaa cactttcgaa
    21901 aaagaaaacg atgtgaaaat caagctagta gaaaaggaca tgttcgatca gttggaatca
    21961 ttagcgttag atgggcctgc tggaaaggga cctgacgtta tgatggcagc atatgaccgg
    22021 atcggtgcgt taggacagca aggacatctg gcggaagtaa aactgggaaa tgaagcagct
    22081 tacgatgaaa cagacaaagc ccaagtgact tatgatggaa aaatctacgg agaacctgca
    22141 gtaatcgaga cccttgtttt gtactataac aaagatttag tcgatacagc accatcaacg
    22201 tttaaagatt tggagaatct atccaaagac agtcgttttg cttttgagtc agaagcagga
    22261 aaaaatacag gatttttagc taaatggact gacttttatt attcctatgg tttaattgca
    22321 ggttacggtg gctatgtatt tggtgatgat ggcacaaatc cttcagatat cggtttgaat
    22381 aatgcaggtg ctgtagaagg aatctcttat gcaacagatt ggttcaaaaa tgtttggcca
    22441 aagggaatgc aagacataaa aagtgcagga gacttcgcca gccagcaatt catgtccaat
    22501 aaaactgcag caatcattga cggtccatgg caagcgcaaa catacaaaga aaacaatatc
    22561 aattatggtg tagccaaaat tcctactcta aataatggac agccttatca accgtttggc
    22621 ggaggaaaag gatgggtagt cagcaattat gctaaaaaca aagacctctc tcaaaaatgg
    22681 cttgattatg taacgaatca ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc
    22741 cctgcaaacc aacaagctag ggaaacagca aaggctaaaa acgatgaatt aacaacagct
    22801 gtgattgaac aatatgaaac agctcaagcg atgccaaaca tcccagaaat gggcgaagtt
    22861 tggacaggtg cagaaaactt gatgttcgat gcagcatcag gttcaaaaac accaaaagaa
    22921 tccgcagatg aagcagtaaa aacaatcagt gaagccatag aacaaaaata ctgattaaaa
    22981 tgataaccgt ccatttttat aaaaaatgga acaggtgctt cagataaaag tggcaagagc
    23041 gagatggaaa attatcttgc tcttgttgcc attatcttaa aagcagtttg cggtttagca
    23101 agtttagagg gggcattcag atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa
    23161 tcacgctttg ttcacttatt ccagggatgg ggcagtttgt aaatggccaa gcttttaaag
    23221 gtatcgtttt tttcgccata ttcgtcctat tcattggtca gatgcttctg ggcggatttc
    23281 atgcattggt aggattggtt actttgggaa gtataccaat ggaagatcat tcattattta
    23341 ttttgattga aggcactttg caattcatca ttacaatcat tttcttgatt ttttacggtt
    23401 tgaatctttt agacgcttat cgagtagcga agatgcgtca agagggcaaa aaaatcaatc
    23461 ggacgatgaa ggaagtcata cataatatag gtgatgaagg ttttccttat ctattgacac
    23521 ttcccgctta tcttctcatg atttttacga tcattttccc agtcctagtt acattgttca
    23581 ctgcatttac taattatgac ttcaatcata ttccaccagc cagtttaatt gattgggtag
    23641 gggctaaaaa cttttttagt atcttctttt taagctcata tcgtaatact ttttttgcag
    23701 tcttttcgtg gaccgtgatt tggactttat gtgcaacaac tttgcaaatc acccttggta
    23761 ttctcacagc ggttgttgct caccagaaat ttattaaatt caaacgtatt ttcggtatca
    23821 ttttcttatt accatgggcg gtcccagctt ttattactat catgagtttt tcgaatatat
    23881 tcaacgatag cgtaggtgcc atcaatactc aagtgattcc tttcataaac cagcttcctt
    23941 ttgtagatat cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta
    24001 tcttagtgca gggctggtta ggatttccct atatctatgt gatggttaca ggaatcttac
    24061 aatcgatttc agaagactta tatgaagcag caaaaatcga cggagcaaat gcatggcagc
    24121 gcttttcaaa catcacgctt cctgctgtct tcctagtggc agcgccaaca tttattacac
    24181 agtatacggg gaatttcaat aatttctcca tgatctatct gtttaacaat ggcggtcccg
    24241 gaagcgtagg cggaggagct ggttctactg atattcttat ttcctggatc tataaactta
    24301 cgactggaac ttctccgcag tattctatgg cttcagcaat cacgttgatt atctccgtca
    24361 tcgttatctg tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat atgggggatt
    24421 aacaatgaaa aaatttcatt cgagtgctcg attccaaaaa cgactttctc acttttttac
    24481 ttactttttt cttttaatac tgtcagtcat tatcatctat ccgcttttga tcacagcaag
    24541 tactgccttc aagtcaggaa atattgcagc ttttagttta gatctttcaa gtgactggac
    24601 tttaggaaac tttactcggc tattcagtga aacactttac gggacttggt ataaaaatac
    24661 attgatcatt tctgtcctaa caatgatcgt acaagtgact gtagtaactt tggcgggcta
    24721 tacttatagt cgctatcgtt ttatcggccg caaatcaagt ttgatgttct ttttagtgat
    24781 tcagatggta ccgactatgg cagcattgac tgccttttat gtcatggctt tcttattaga
    24841 agcgctcgat aagtactggt tcttgacaat gatctatata ggcggtggaa ttccaatgaa
    24901 tacttggctg atgaaaggat actttgacac tgtaccaata gatttagatg aatctgctaa
    24961 acttgatggg gcagggcatt tcaggatttt tttccagatt gtattaccgc ttgtcaaacc
    25021 gatgctcgcc gttcaagcat tatgggcttt tatgggacct tttggggatt tcatgttagc
    25081 taagttcctg cttcgaacac ctgaaaatca gactgttgct gtcggcttac aaacatttat
    25141 ttctgatgtg acaaaccaaa gagtctctct ttttgctgct ggggcaattt tgattgcact
    25201 accgatttgt ctattattct tctttttaca aaagaacttt gttaccggcc tgacatctgg
    25261 cggaacaaaa ggataggagc agagtatcat gcaaacattt ccgttaaact atttttccgc
    25321 attacgtacg ccaactcaag tgtttgcggg acgcaaacta ttatcatggc ctaaattttt
    25381 cttgatattt gtatttttag tttccttgat ggtcatgcct gtgactcttt tttatgccaa
    25441 tcagatccaa gcgattccat tagaacaatt tctatccgtt cattcgctga tcgatgaaca
    25501 aggaacccaa aaattttctg aacttgaatt atctgaaact gggttacaag catcgcaaca
    25561 aacgatagct gtgacccctg aaattttggt aggtgtgtct ttatctgaga aacaacaatc
    25621 cgatcatgga acttttattg attttgagaa ggaacaatgg gtgatccaac aaaaggataa
    25681 atctggtata cgccgctata cgatgaatta tagcccatct ttccaaccag attctgtacg
    25741 tacgcccgaa gatttccaac gatttttaga aagagaattt tatgccagta atcgtccaac
    25801 aatcatttta tcttatagtt taagtcttgg tcttgtccta tttgtcatga cgagtctcat
    25861 tttattcggg gcttcctttt tcttgtggat gacaagaaaa agccaacttt catccattca
    25921 tacattcaaa gagagtgcaa atttgatgct gaatgttatg ggaataggtt ccatggcagc
    25981 agcagttgta gggtttatcc actttgactt tatcttgatg ctaagtgtcc aaacaacgat
    26041 aactgtcttg ctgcttttat gggtttttgt caaaacaaga ttcaaagata agcgtgtcga
    26101 atgacaagtt taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt
    26161 ttttgaaaaa tttctgaaaa aattaaactt cttagttgat tcttttcttt tttagtatta
    26221 acgtaatagg gtagtttttt taaggaggaa aatcatgtta gagaaaactt tatacaatca
    26281 attattttca cgttctttct cgctccctgt cgaagtaaat tattgggacg gtacaacgaa
    26341 acaatacgga gatacggaca ctcctcccca aatcaaaatc aatatccatg aagaaatccc
    26401 aatgaaagag attacaagta acgcatcttt ggcactagga gaagcctata tggatcatcg
    26461 aatcgagatc gagggaagta tccaagcatt gatcaacgat gcttatcagc aagcaaacag
    26521 ctttttacga ggaaaagatt atctaaaatg gctgccaaag aaagaaaaac atacgaaaca
    26581 aaaaaacaaa gaagatatcc atgcgcatta tgacttaggt aacgactttt ataaactttg
    26641 gctagatcca actttgactt attcttgtgc ttatttcaat acacctgaag atacgttgga
    26701 acaagcacaa atcaataaag tccatcatat cttagacaaa ctctttatca aagaaggcga
    26761 aacgttgctt gatatcggtt gtggctgggg aacattgatg ttcactgcta ccaaagagta
    26821 taatgtcaag gcaactggga tcacactaag tgaagaacaa tacgatttga ttaccaaaag
    26881 aataaaagaa gaacatttag aagataaatg ccgtgtatta ttaatggatt accgcgaact
    26941 aaaaggcgaa acgtttgatc atatcacaag tgtaggtatg ttcgaacatg taggttctga
    27001 aaatcttgaa ggttatttca aagtggtaaa agatttattg aaaccaaaag gaacagcttt
    27061 gatccatgga atcagccgac aacaaggcgg tgcgacaaat gcttggatca ataaatatat
    27121 tttccctggc ggttatgtcc ctggtgttac tgaattagtt gataatatta caaaaaacga
    27181 tttgcaactg attgatttgg aaagcttacg tcgtgactac caattgacat tagaacattg
    27241 gacaaagaat tttcatcaag tccaagatca agtcacgaaa gaaatgggtg attccttcta
    27301 tcgaatgtgg gatctatatt tacaagcttg tgcagcttca tttgaatcaa gtaatatcga
    27361 tgtgattcaa tatttgcttg ttcaacctgg aaacaatgac atcccaatgc atcgtgcata
    27421 attgaggcaa aagtactgtt taaagactat ttaaaaagaa gctaagtttt atgatgctcc
    27481 agaagtgaac ttttttgtct aacttctagg attgtctcta aagcttagtt tctttttctt
    27541 tatattttca taataaaagt ttggttcttt aatttttaaa catgacagaa aattttttct
    27601 tcgttatact tgtaatagaa tactatttta taacaaaggg gatcgaatca atgaaaaaaa
    27661 ctgttattta tactagttta tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca
    27721 atcaaggcac atctgggaat acgtcttcca gtagcgaaca gcgctcttca caggtatcgg
    27781 actcctcaac aaaagcaagc acatcttcgt ctagccagac aaaaagttct agtgaagcaa
    27841 gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc caagataaat
    27901 acccagatac tgcaattact agtttggaat tagatagtga ttggggatct tacttctaca
    27961 agatagaagg tgtcgatgat caaaaagaat acgaagcaag aattgatgct caaaatgaaa
    28021 aaatggagac agagaatcca gaaactctag atcgagatga acaaaatggt caaaagaaaa
    28081 aagaagatgg tctggatgtt gataaattga tcacgattga ggaagcagca aaaatcgccg
    28141 tcgacaaagt aggaagcggt acggcaacag attgggattt ggataaagat ttaggtacta
    28201 cttattggga agtcaaagta aaagacggca atcaaagtac atcagtcaag atcaatagcc
    28261 aaacaggcga agtactgtca actgaacaag atgattaaga atttcttcgg aaaacaagag
    28321 ttgatttttc ttttagtcat gatacactag acaaaataac ttgtttgaag gagcgattta
    28381 cttgagttta gaaacgacct ttgccaatca tatctttgcc aatcctttga tgaatgcatc
    28441 tggtgtccac tgcatgacta cccaggaatt agatgaactg gctcattcag aagcaggagc
    28501 atttatcact aagagctgta cgatcaatga acgaaaagga aatcccgaac cacgctattt
    28561 tgatgttcct ttagggagta tcaattctat gggacttcca aacttaggtt tttcttatta
    28621 tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac cgttattttt
    28681 ttccattgcc gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag
    28741 tgggtttaat gggatcactg aattaaattt atcttgtccc aatgtgcctg gaaaaccaca
    28801 actagcttac gattttgagg ccacttatga aacactgaaa gaagtattca gcatcttttc
    28861 taagccgcta ggaatcaaat tgcctcctta ttttgatttt gctcattttg atcagatggc
    28921 tgatatcttg aatcagtttc cattgaccta tgtcaatgct atcaacagtg tgggcaatgg
    28981 tttgtatatc gatacagaac aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg
    29041 cattggcgga gagtatatca agccgactgc tttggcgaat gttcgcgctt tttatacacg
    29101 tttgaaacca gaaatccaaa tcattggtac aggtgggatt cggacaggac aagatgcatt
    29161 tgaacatctg ctatgtggcg caagcatgtt acagattggt accgagcttc ataaagaagg
    29221 gccggaaatt ttttctagaa tcataaaaga attaacgcag attatgtcag aaaaaggata
    29281 tacgtcaatt gacgaattta aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg
    29341 tcatgattga tcttcgtctt tttttataaa aaacactcgt ctgttcattc ctgtgcaagc
    29401 gtactcattg tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat
    29461 atgaatgatg caacagaatt agcaaaagag cttaaagaaa aagaatttag ttttcaagaa
    29521 ctagttacgg atatacagaa aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc
    29581 gcgcttgaac cattcgagag taattcccac gagctattga ttccccagtc agaaaaaggg
    29641 ccattggctg gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta
    29701 gctacttcag gatctcgttt atttgaaaca caccgagctt ctcatacatc taattatgtg
    29761 cggcaagcag aagcgatagg tctagttcct tttggacaaa ccaatgcgcc agagtttggt
    29821 ttcaaaaata ttacagaccc agtcatctat ggcccagcaa gaaacccttg gaaccttgaa
    29881 tgtacaccgg gcggatcaag tggaggagct gctgctgcag tttcatcagg aatcgttcct
    29941 ttagctggtg ctagtgatgg cggtggttct attagaatac ccgcttcgtt ttgcggattg
    30001 attgggctga aaccttcgcg agggacgatg ccagttggtc catatgcttg gagaggatgg
    30061 caaggagctg ctattgattt cggcttgaca gtttctatga gagacacgga ggctttattt
    30121 tatgggatga gaagtatcca ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa
    30181 acacatccaa gaaaaaaacg gttgaagata gctttttgtg ttgattcacc gatccactca
    30241 cagattagtg aagaggcagt acttgctgta aaaaaagctg tagatttcct tggaaaagaa
    30301 gggcatgatt tgacagagat tccttaccca ctagatggtc gtaaactgat cacgtcttat
    30361 tatcagatga atggcgcaga aacagcagca atgatccgct cgattgaaac aggcatccag
    30421 cgagctgtca gaaaagaaga acttgaaccc atttcttgga ctttgttcca atatggtcag
    30481 aagctctctg ccgctactta catcgattct ctgcatgtat gggatgaagc agcaattgtc
    30541 atggaaaacc tacatcaaga atacgatcta ttcctttcac caacgactgc atttaccgcc
    30601 cctaaaatca gcgaagattt gcaaagtgat gcgataagag cacagatgga acacatcagt
    30661 gattgttctg aagtagaagg tttagacgtt gtttcttcca tgtttgagga gagcttgaag
    30721 atcacacctt atacacagtt ggctaatctg actggacaac cagcaatcag cttgcctact
    30781 catttgacaa aagaaaacat gcctctagga atccaattta tggctgccag aggaagagaa
    30841 gatttgctat ttcaaatcgg ctatctattt gaagcaaatc atcagttcca tttaccaaat
    30901 ccatggaaat agcagaaaca aggagaagaa gaatgaagaa aaaaagattg tttgctctaa
    30961 taactagtgc cttttttatt ttaagcgctt gcggtacagc tgccgctaat caggaagata
    31021 acagctggag taatgtagaa aaaaagaaag aattagtagt agcgacttct ggcacactgt
    31081 ttcccgcatc ttattataat gatcaaaatg aattagttgg ttatgatgta gatttggcaa
    31141 aagaggtggc taaacggtta aatgtcaaaa tctcctttaa agaatacaac gtggatggac
    31201 aaatcacttc tgtttcaaaa agagaatcag attttgctgc gaacgatttt ggtatcacaa
    31261 aagaaagaga aaaaaaattc tccctttcag agactttcaa atattcattt gacagtatga
    31321 tcgtacgaaa aaaagatgat tcaggaatcc actcacttga agatcttaaa gggaaaaaag
    31381 cagctggcga acccaatact agctatatgc gtttagcgga aaaacacgga gctaaattag
    31441 tcacatatga caatgccacg aatgatcagt atttaagcga tgtagcaaat ggtcgaacag
    31501 atgttattct aaatgattat tatctgcaga aaatggccgt atctgcttta ccagatatcc
    31561 ctgtaaaaat actggagaac gtttatttca atgaaaacga aagtggtttt ttattgaaag
    31621 aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg aaagaagatg
    31681 ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag
    31741 aaacgatggc tgagtcggtg agtgagtgat gtactttcct ctaattgatt tttccctcat
    31801 gtggacttca cttcctttcg tactgcaagg cttggtatac acattaggga ttggcttcgt
    31861 gtcgtttctt ttaggaaatc tggtagggtt gcttttgaca gttttaggtc ttttggattg
    31921 gctgcctctg aatgtattta ttcgttttta tttatcgttc tttagaggca ttcccgcact
    31981 agtcctgctg tttctgttat attttgggtt accttatcaa ttaagtgcct taacggcttc
    32041 agtcatctgc tttacgatta ccagcagtgc atttattggt gaaatctatc gaggatcact
    32101 agcaggcgtt tcttcaggtc agtgggacgc tggtttagct tctggttttt ctttctttgg
    32161 ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa
    32221 cgtagctatg gatctagtga aaggtacttc gttagcagca acaatcacag tgccagaaat
    32281 atttcaaaaa gcaaagatca taggcggtcg gacattcgat tatttttctc tctatatttt
    32341 agttgcactg atttattggg tcatttgtat cgggatcgaa aaagtgcaac agtatttaga
    32401 aaaacgttct atgcatcagt caatctgaaa aatagaaatg gtgctagcgt ggctggcacc
    32461 atttctattt tttgtttctt gtatattctg cctagtattt taaaaccatt tcatgtaaaa
    32521 tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga ccccgctaac catcgaaata
    32581 gaatcctcgt tataccaata ttgcatcgag caaggcggct tggttgtaga agaagtcacg
    32641 atgcctgatg accaaggaat tgtagatacg cttagctgct tgacaaaatt tgatggaagc
    32701 cgagaatggc gatgctatga gctgaaagtg acaaaagcag attttcgctc tacagccaaa
    32761 ctgtcatttg ttggacatta taattatttt gttttaccaa gtaaactata tgaagaagta
    32821 aaaactgaaa tccctacagg aataggtgtt ttagtgtatc gtccgtacac ggttgaagag
    32881 gagatgcctg cctctggtac atttctgatt gcgaaaaaag cagcaaagaa agagcttgct
    32941 gtaccggaag aagctttgac aaaccgcttc atggcttctt tattcaggga agtaagaaaa
    33001 gcaaagcaaa tggagcgagg cgtaaaatat tttccaacag atcaattgta caaagaattg
    33061 aaaagacgtt cagaaaatca agatccattt tctaacgaac gatattatga acggttttta
    33121 gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc gcttcagcat
    33181 gattatgaat atttgaggca acaacagcag atccgtcggt tgcctacaga gccattggag
    33241 tgaaaaatat gtactatcct tatttaagag gaaaacaatt tgacctatta gcattaaaag
    33301 aagcactgtc gcgaggattg ctctccaata aaatccaacc agtgatcgaa ccagtacgtg
    33361 attccgctac gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat cgaatcgctg
    33421 tgatccaaaa tccccaagta ggacaattca aattattcga tcagcatgtg cacagttgga
    33481 cagtcaaaga aaatagctct gtcgtccctg cacagatctt aacgccagaa aatctcagtg
    33541 aagtactgga ttcacctcca gcgtttcttg ttttcgatgg acagcactac ccaaaagata
    33601 cagaggtgtg gaaacagttg gcaggagttg attcgaaatt tttgatacca gatactagtc
    33661 gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa
    33721 agcatgttga aagttatgca gataaaacag atgacttttt tagtgatgac tatctttttt
    33781 tccacgcaga cggatatatt ggattttctg atttcacgat cgaaggtagc cgttatttcg
    33841 ataaagggtt ccctagccga gcgcttgcta tccatctaac gtatattgat gcttacggca
    33901 atatacgcgt gaaacatttt gtctctgaca gtaacgactc tgctaaagac caagcattga
    33961 agtttcttga agcaggagat aaaatgaaaa aatggatcat gcgtcaccat catcaactct
    34021 tgattacttc aggaatgatt gaattattgg ctctttacca gcaacaaaaa ttccctggac
    34081 tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga acttattagt cacctgctag
    34141 atcatccaaa agattggctt aaatattatt caaaagttca ggaactatat gaagtgatcc
    34201 aatgattaga taatgtaaat gaaaaaggaa tagaaaagag gaaactggat gtatattcgg
    34261 caaacaagga aagaagaagt agaagaagtg gtagaaatca ttaatgccgc agcggatttc
    34321 attgcttcgc aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt
    34381 ttgaacgata ttgaaagaaa agaaagctat gtattaatag tggaagatca aattgctgga
    34441 acagctgcat tagtctcaag cgtggatccc gtttacacaa aaatagatgg tagttggaaa
    34501 gaatcaactg gtccttattt atcgattcat cgtgtcgctt tagcagaaag tactcgagga
    34561 aaaaaactag caaaaccgtt cttagaacat ttgttgacga ttgcagccag caaaaatatc
    34621 caagatatcc gtatcgatac ttatccagct aatgaaccga tggaaaagac catctattct
    34681 gctggattca cttatcaagg gatgattgaa tttccttttg aacacggcga aagaaaagca
    34741 tatcaaataa tcatcaattg actataaaat catctgagca aagataaaga cattaacact
    34801 actgaaaaaa acaatcatat gatagccaag aggttgtaac agaagtggcc agcttcaaaa
    34861 aacaagaaag aatttccgaa aatgcttctc aaatttttgt ggaattttag cgtattttac
    34921 gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg tgacatagct tttgtcacga
    34981 cctctcggct tatcctacga gtattattcg atacatttat atcaataatc ctttttttat
    35041 tttatctgca gcttcttgga acgctccatc gtttgacacg actaaaagtg aggggatagc
    35101 tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc gccagtctgg ctgaaatctg
    35161 ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat gtttctttag agatcgtaat
    35221 aaagatagga cggactaccg tcccgaaata ctcatataaa tgccaaaaac catttgctgt
    35281 caaaacggcg taacaatttc ctactgtcag tttcttttta atttctgctt tactggaacc
    35341 atatttatgt tgagcatatt ggtcccactc tgcaaactca tttcccttga tcatctgatt
    35401 gaatgtattg tcagaaacaa aataataatc ctcttgatct ttttccccag gtcttggtga
    35461 tcgattcgtg taagtaatga tcttttgttc cggctgaaaa atagcttctg cgagtgaagt
    35521 ttttccagaa ccagaaggac cgataaatac atagcaataa ttcattgtca tttctccttt
    35581 gatgattgaa caagtgaaaa agctttggaa aatatttcca aagcttttaa aagtaacttt
    35641 gcttttttgt atattatacc agcaagccag tcacaatagc acttaaaaag ctgactaaag
    35701 tcgctgttag caaaattttc aatccgtgtt ttgcgacaag atttcctttt tcttcattta
    35761 atcccttcat cgcaccagaa atgatcccga tagaagagaa gttggcaaat gaaacgagga
    35821 agacagatat aattgctgtt gtacgctcag taaaatggaa attaccattt gctaattctg
    35881 tcatagcaac aaattcattt gtaaccagtt tagtcgccat gatactaccg gcatccactg
    35941 cttctttcca tgggattccg ctgatgaagg caagaggggc aaagatgtaa cctaggatct
    36001 cttggaaagt gatgccgaat atcccgtgga agatagcatt gatcatggca atcaaagcaa
    36061 caaaaccgat aagcatagca gcaaccgtga tcgccacatg gaaaccatca agaatgtatt
    36121 ctcccaacat ttggaaaaat gtctgttctt tgttttcttc aatgatcagc tcatcttctt
    36181 tttcttctaa tacataggga tttacaattg aagccagaat ataaccgcca aataagttca
    36241 aaacaagggc agtgatcaca tatttagaat cgatcagtgc catataagat ccgacgatcg
    36301 acatagaaac tgtagacata gccgatacac tcatggtgaa caaacgtttc tcagatagga
    36361 agggtaattc cttttttatg gaaataaaaa cttccgattg tcctaaaata gcagaagcta
    36421 ctccattata agattccaat ttgcccatcc cgttgatttt acttaatcct agaccaacag
    36481 ctttcataaa taatggaaga atcttgatat agcgtagaat accgataata gcagaaataa
    36541 atacaattgg catcaatacg gaaagaaaga aaggtgcact tcctttattt gctacaccgg
    36601 caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag atgatcaaac atttttgcca
    36661 atacagaaac aaccgctgta ccaaaagttg tacgtaataa tacgaaacca agaacaagct
    36721 gtaaaacgaa catcacaatt agtggtttca atttgattgc ttttttgtta ctgctgatca
    36781 aaaaagcaag ccctaatgtg acaaataaac caataatacc taataaataa ctcaaataat
    36841 cactcctatt ccaaattccg ttttatttta cctgttttgc aaataaaata ctaatcttta
    36901 gtgaaatttt cattactttt ttagagtgaa acgcattcat tttccattag aaaataaagc
    36961 gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac
    37021 tatcgattaa gctaatagtt tcgttttcaa catttataag taaacttttt ctattggtaa
    37081 tatagatttt cggaagggta gacaggtgcg caggtaatat cgattcctag ttttttcaat
    37141 gtttgttcat catttttatt gaccatcaca gtagagtggg cttgtgctcc ctcaagttca
    37201 gctagtttat cataagcaac ttgagcgatt ggattcgtta ctgcactgat tgataaagcg
    37261 atcaagactt cattgatgct tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat
    37321 ccttgtattg tttcaagaac taatggtgac aataaaggaa tgtcatctgc gatatgggct
    37381 aaatgtttta ctgcatttaa gataactgcc gctgacgcat ccatcaatga acttgtacgc
    37441 ccggtaatga tgacttcatc tggtaattcg attgcaatca cagcagaagg atcttttgta
    37501 ggactttgcg cttgaatctt atctgcatag tcatgggcag ggactaccac gtttcggtct
    37561 tcaggacgta aaccgacttc ttccataatc agtttgattc gattaactgc ttcttcgtcg
    37621 atcaatcctt ttttgaagtc gcattctgtt tggaaataac gacggatgat ttcttgttta
    37681 gaggcttctt ggacaacggt atcatccacg atcccaaatc ctacacgatt cacacccatg
    37741 tcagtaggag attgataaac agattcttta ccagtaattt tttcaataat acgtttgatt
    37801 acaggaaatg tctcaacatc ccgattatag ttcacagcta ctttattgta ggcatcaaag
    37861 tggaaggaat cgatcatatt gacatctttt agatcgactg tggctgcttc ataagcaatg
    37921 ttcagcggat gtttcaacgg aacattccat actgggaaag tttcgaactt ggagtaacca
    37981 gctgcatgtc ctttttggct ttcatgatac agttggttca aggaagtagc gagctttcca
    38041 cttcctgggc caggagctgt cactacaacg attggtttag ttgtttcaat atactcattt
    38101 tttccaaaac catcttctga gacgattttt tctacgttga ctggatagtc ttcgatttct
    38161 gagtgtttat agaccttgat gtttcgtctt tctagtttat tgataaagac tttcgtggca
    38221 ggctgtccac tatagcgagt gataacgaca ctgttgatta ataagccata accacgtaat
    38281 tcatcgatta accgcaagat atccatatca taagtaatcc cataatcacc acggatctta
    38341 ttacgttcaa tatctccagc atagacgcaa atcagtattt cagcttgatc tttcaatttt
    38401 tgcagcaatt tgattttcgc atcttcttca aatcctggaa gtacacgttt cgcatgcttg
    38461 tcgtcaacta gcttcccacc aaattctaaa tataatttgt catagtggtt cactcgttcc
    38521 aaaatatacg ctgactgctc ttcaatatat ttttctgaat caaaaccaat ttttttcact
    38581 aggttccctc cttaagtact aaagcatttt ttcatttttt tgaggattcg tcaactggaa
    38641 gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct agatagttat
    38701 tcattggtat gatagttttt gataaggaag gtgctacatg aaaaaaagac agaaaaagaa
    38761 aaacgcatat aaacactata tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga
    38821 tcccgaattg gaacaattga cgtttactta tctaaacgaa gaaacgcagc taactcgtga
    38881 tgagcatcag cgcattcatt tcacaacaag agatctgcct agtaaataaa aaataagata
    38941 aaggtcatcg gattcgattc tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg
    39001 ctgtaccaaa ttttggtgca gcctgtttta tggagcattg cccgctcttt tttataacaa
    39061 ttttttacag aaaatggtac tattgaagta ttaataagtg aaaaacagat gagtaagagg
    39121 aggcaagtag tatgaaagag ccagatacaa atgcaaatta tgagccagac gaactagagg
    39181 agacgaatgc ggatcaggat acaggacaag aatcgtcttc tttttacgat atcaaagaaa
    39241 aagcaagcat gaatctttcc caattattag aagacttaca ggcttttgaa aaagctgtcc
    39301 gcgaagaaga tatccctgag atctaccgtt tatacaatgg ccgattgaat gaagaattaa
    39361 aaagaagctc aaatgaaaat cacgagatcg atcaattgct gatgcgtaag atccatgatc
    39421 gttttttaca ggaatttcct tttatggaac aagtagaaaa catctcgcca acgatgagct
    39481 attacaaaat cggcacttat taccatgatc ggcccacggt cggtattgat gcaagtctgc
    39541 cagaaatttt tgttttgcct aagatcgatg aagagtggga gaaatattcc caaccggagt
    39601 caacggacta tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact gctcagacag
    39661 aaattgaacg tttagatgaa caaataaaag aaatcaatcg acaaatgact gatttgaacg
    39721 acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga
    39781 agaagcaagt actatctaat gaaaaattag gctggcttcc ttatattgag acgcctgaaa
    39841 caatccagca gcaaaaagaa gcattgaagc aagaagcaag agcagaccag cttcgtgcag
    39901 ccatcgtaga aaaagaacaa aggcaaatca gacgctattt cggtagcaaa gagggattcg
    39961 gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa tgagaatcct aataatcaat
    40021 ctaatgaagg aggttccgag tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa
    40081 gaacaaacag aagatcatga agaaacgaac acttcattgg aagaacaaac aagtaatcct
    40141 ctggatgaca cagaagaaat ccctatgcta gaggaaacaa aaatacacga aaaagaaaaa
    40201 gtaactccga cgaatacgaa tactgcagaa aacacccatg cttacgaaat aactgaaaat
    40261 cccgggtatt tagaaggaaa agagcaagat catcaagata tcgtcagttt gattgaaaca
    40321 gcggaagcag aattagcgga tttacgtatg agaaaagatt tgatcgaatc agattttcca
    40381 caattattag aatcatatga tcgttttata ttgaatgaag aaccaatctc cacgatcgga
    40441 aagacaagcc tgatcgaata cttcacgtat gaattactga aaccgttgaa tgatattggt
    40501 ctggaacggt caaattcagc ttatgacaca tggaaaacaa gacatttttc gatcaattat
    40561 acgttgaatg gacaaaacga attagtcttt tcctttgtat taccgaccat cgatcaacga
    40621 taccaagtgg aatcgatgag tctcctaaac gtatcaccag agacaatgga aatcaatgtc
    40681 caagatgacc gggtgctttc tttgatccga cactggtcag tggatcgagt attctctgca
    40741 gggcagatca cgatattcaa tcacaaattg aatcaattat tagcccatgc aagaacactt
    40801 ggttttaccg ttaatcagac attactagac aatacgaagc cacttcgttt gaatcttcag
    40861 agcgaatttg aattgacgaa tgaagtattg gatgatattt ttatcgtggc aatgaaaaat
    40921 ccatcctacg atatggaaaa gatcaacgaa actacttatc aagtacgatt agataaagga
    40981 cagtctttaa cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga
    41041 gactacccac gatctgtcat tgatttcttt atcaattacg aattcttggt cccattaatg
    41101 gtgagaaagt cctgattgga taagttggat taccagatgt cattcagata attctgttcc
    41161 aaaggacttg cggcagttat ccagaataat tgtcacaagt cttttctgta tgtactaaat
    41221 ggacgtgaat aacttgctga tgaaggaggg atttcattag gaatgtccac aaataaaata
    41281 gcacagctta gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt
    41341 ccgaatttcc agccaatgac tgctattttt ttatttatag tactttactt aagcttaaaa
    41401 gatgcgttaa ttgtcatgag tctttctatt gcgatcagca gtttttattt tggtgttgga
    41461 ccatgggtca tcggccaatg gatcagctat actgttgttc tgagtttgtt ttcaatcgtc
    41521 tgcttgagaa aaacagtgca aaggaatcta tggttaaagg gagtctgttt ttttctggcg
    41581 ggcatggttt acggaatagg tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa
    41641 ccttggatct attaccttca aggggtgtcg tttgatttga tgcacagtat aggcaatgtt
    41701 gtgttctttc ttgtttttct gccagtcgtc aaacgttttt acaatcattc aggaggaaaa
    41761 aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc agtatactca ttttgacaag
    41821 ttgcagtaca acctatacac aatcggcttc caaccaaaag gaggaagtaa aaacatcgca
    41881 aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag aagtgttctt
    41941 caaaaaaggc gatgatttac tgagtatttt gaaacgaaat tttgaagtca aagaagataa
    42001 cggcttcatc acttcgcttg aagggcactc acaggatgag aaaaatcaac tctattggat
    42061 gtttactgtg gacgggaaaa gtgctactac tggtgccaaa gagatcaaat tgaatgatgg
    42121 acaatcggtc attttcaatt tgtctaaact ataagtttca taaaaaactg ttaaagggcg
    42181 aatattgcca taattcagtg agttcagttg atgaaagaag gaaaataaca tggaaaattt
    42241 taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg gctaatattc tagttgctat
    42301 cagcaaattt gtcggctatg caatcagtgg cagtgccgcc atgctaaacg aaagcatcca
    42361 cagtatcgtt gattgcagca atcagatttt gctgttattc ggtgataaac aagccaataa
    42421 aggacagagc gagttgcatc aatttggtga aggacgagca aaatactttt tcagtacaat
    42481 cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa
    42541 gttgacgcat ccatcccatg aagtgggtaa tccgatcatt gttatagcaa tactgttatt
    42601 tggtttggta atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca aagaattgaa
    42661 tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat
    42721 tatttttacg gaagacttgt gtgctgtaat cggtttggtc ttggctttag caggaacaat
    42781 attgacttat gcaaccggga ttgctgcatt tgatgcgatc agcggattgc tgatcggttt
    42841 cttgctgatg ggagccgcca ttttcctagc aaaagaattt tatagtctga tcatcggtga
    42901 aagcgtgaca gcaagcgatt tatcaaaaat caagttagct tttgaacgtc ctgagatcag
    42961 tcggctgatc gatataaaaa cagttcattt aagtccaacg gagattttag ttgcagcaaa
    43021 agcagatatc gttggtaaca aagagaatga agcttactcg gtcatcaatg atattgaaaa
    43081 aaatatgcgt cagtctcttc ctgacaagaa gatgtatata tacattgaaa cagataaata
    43141 tgacccaaat tattctcgta aataaaacgt ttattcatga attgaagcaa aaatgagaaa
    43201 aatataatct tgtttttaaa taaaacggct acaatttagc gaatttatgt tacaatgagt
    43261 ccgctgttta tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc
    43321 atggcttcaa gctttgcatg cagtgatgcc actttgtata agctatatac cagtaggatt
    43381 agcttgcgga gtattgttgc aaaaagttgg atttgatcct ctcttatcag gactgttatc
    43441 gattctggtc ttttccggtg gcgcgcagtt tttagttgca tctatgctta cgacgcaagc
    43501 atcatttgca acgacattat tgatggtttt ctttttagaa ttacgctata cattattagg
    43561 agcaagtctt gccggtttta tgaaaaaaga aaagcggacg tttttggctg tcttttctca
    43621 atcactgaat gatgaaaact atgcggtgaa ctacttaaag ttttcaactg acccttcctg
    43681 gaataaaaga aaggctttat atgtcaattg gttctcaatg actgcatggg ccggtagcaa
    43741 tatgatcggt aattttttcg gctcggtgat tcgagtagat gctgaccttg tccattttgc
    43801 tttgacggcc atgttcattt tcatgtttat catgcagatg aaaagcgcac tactgatctt
    43861 tacaggttta ttctcaggcg tattaggggt cgtattcatg gtattattcc aaaatacttt
    43921 cggattgatt gcagcaacag tcattgcttc atttgccgga tttatgctgg agaaggcatt
    43981 caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg agccgctctt
    44041 caacttcccg gaacaggagg ccaatcacca tgactaaact atatttgcta cttttagttg
    44101 cttgtctgtt catcgtggca tatatcccgc gcttgttccc gatgctttat tttactcatc
    44161 gaaaagtgcc atcctggttc agtgactgga tgaaatatgt accggtggca ttatttgcgg
    44221 cgttagcttt taaagatgtt ttcattactc atgaacatct ggatattgct tggaatatca
    44281 aaatagctgc aatgattcta gtagcaggcg tggcttacaa aacacgttcg atggcgttgt
    44341 cagttttgac aggacttgcg tctgtctttt tgctgtctat gctttaaact gttttatttt
    44401 attaaaaaca gagattgcga cataagttgt tcaaactgat ctatccgaac tgcatttgca
    44461 acagtcaatt ctggatgcaa gtttattcgt agggataaaa aagattagtg ttggcttatg
    44521 atgcaagctc ttttttattt taggaattag agagggattt gtccttctat gattttttgc
    44581 tataataggg aagatgttta ttttatgaaa gaatagttga gggaatttaa atgaataaaa
    44641 aattgatgca aggaacattt tggctaacct ttgctaatct tctatgtaaa gttttagggg
    44701 tagtctattt gattccctgg ctaagtatga tggggaacaa tcaagatggt atgcttgcta
    44761 cgacattgta taatgttggc tatcttcctt atggattgtt cctgatgctt ggtacagtcg
    44821 gatttccaaa tgcgattgca aaaaaagtag ctgtagccac aaaagaagga gataacgctg
    44881 cttgtcggct gatttttaga agtacgatca atatcatgtt tgtcatcggt atcgcctctg
    44941 ctgcactgat gtatttattt gctcctttac tagccggaat cagtccgatt gctaacgtta
    45001 ataatgggat actagctatc agaagtttat gcccatcttt gattgctatc ccaattctta
    45061 gtgcaatgag aggatatttc caaggaaaaa atgatctgcg gccttatgga acttctttga
    45121 ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg tacttattac ttgcgcgtat
    45181 tgactgacgg aaccatatta gaagctgtat tgatcagtac agtcgcttct ttcttcggtg
    45241 ggttagctgc gattatccat atgtatatcg tcggacgcag aaaagacttc ttcgaactta
    45301 aagatttttt gatttccagc cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta
    45361 tcattcggga aacactaccg tttatttttg tcggctcagt cattactatc gttcaaatga
    45421 tcgaccaagt aacgatgaaa ccactgcttc actttttccg tccagagatc gcagatcagc
    45481 aactggaatt cttattcagc cgtgcttctg tcaatccaaa taaattgaca ttgatcctga
    45541 tctccatggt aggaacagta gctataagca gcttgccaat attaagcacg atgaagaaaa
    45601 aagatcggat acaaatcgaa aaaacagtag gagatagttt ttcgattgcg ttattgattc
    45661 ttcttccttc attgactgga atgtctttat tagctggacc actatacaca ttgttttttg
    45721 gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt gctagcttct ttgtttttct
    45781 ctttattcac gatcctatct acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc
    45841 gattaactgt agaagcaatt atcctgaaag tcatcttcca agtaatcgga ttaggattag
    45901 ttggcggata cgggatgagc ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg
    45961 gctatcactt tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc agcaattttt
    46021 tcttaaaaac tttccgctcc accctgatta tgcttgcagt atgttttgcc gttttctttt
    46081 tgctgaatca gcgattgtca atggaatcaa aaagctatgc aatgatttat tgtgtggctg
    46141 taggaagtat tggcggctta gtatttttat tttctcaatt cggaaaaaat gggtttcgaa
    46201 tgttgaaaaa tgttaaacat catagcagaa gcaaagaata ggttaactaa tcgttttttg
    46261 ctgtcacttg aaaaacggca actcgtatga ttacgcttgt cgtttttcta ttttccaatt
    46321 ttagtttcat atctaatctt acttatgata tagttataag gtctggaaaa attctttgaa
    46381 acgaggaaat taaacacatg aaacttgcag tagttacaga tagttcggca tatctgccgg
    46441 aacgtatcaa gaaccatccc gacttattcg ttattccaat ccctttgatt atggatggga
    46501 aaatttataa cgaaggaatc gatatcgaag cggatgaata ttatggactt ttaaaaaaca
    46561 gtaaagattt tcctactact tcacaacctg tcgtagggga agttttgggt ttatatgagg
    46621 aattgaaaga gaaagggtat gacacgattt taagtatcca tctttcttca gggatttctg
    46681 gctttgtcaa cacattattt gcgatgaaag atgacatcag cggagtaacg gtcattcctt
    46741 atgactctaa gatcactagt atgccgatgg gacacatggt agaagctgcc ttagacttaa
    46801 atgatgaagg tcagtccctt gatgaaatac ttgcacatat cgatcgaatc cgagataata
    46861 cctacgctta tctgattgta gatgatttga acaatttagt acgaggagga cgtttgacaa
    46921 atggtgcagc gctgattggc agcttattaa aaatcaaacc gatcctgaca ttttctgaag
    46981 ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa agcatttgct cgagctgaag
    47041 aaattattgg tagaagaaat aacgaaatac aaccgccagt caaactttac gtgatacatg
    47101 caaataattt agaagtagcg attgcggaga aagagaaatt gcagaaaaaa tatccaaatg
    47161 cagaaatcga aattggtcat ttcggaccag tcattggtac acatttaggt gaaaaagcca
    47221 tcggcattgc catttcagcg caataattcc ttttattcaa aaaacatacc aaaaatattc
    47281 caacatcatc ttggactttc tacatgttac taatgagcgt gtaaaagaac gagatggtgt
    47341 ttttatttct atcaatgaag ctctttttct ttgcaatcct cacttcaatc ctctataatg
    47401 tcgcatatag tttagtaaat gagtaaacta ctaatttact tatttactaa aaataaaagg
    47461 atgaggagtt aaaataatgg aaataccaac aaatttaaaa cataaaccag taatcgttgt
    47521 agaagactat gataaagttg atggaagaaa tgcattaaat acggatgcaa aagggctttc
    47581 actgggcctt gctcagtgga atgatcgtgg aaaagtcgat atctcaggca aggtatggcg
    47641 acatactgga gaaaaatggt cacgacagtc ggaagaatta ccaatcacac gtattttaga
    47701 tttagcgatc ttagctgcac aaggaagttt atacttccaa gatgcttatc gttatgaaaa
    47761 attttatgat ccagaaaatc cagtcgtaga catcattgga ttacaaggga accgaatgac
    47821 ggtagaagta gataccaaaa atccaaagat cgatgaagac atcattttgt attacgatac
    47881 actacaaaaa gacggagaac tgattgggga acgattccgt attttaaaac gtcttttaga
    47941 tgagctagga tattaataga cagagatgat caaaagggga agagaagcat ggagaatttc
    48001 tcactaacag aaaaccgaac cttttcacgg ctagaaaagc aaatgatctg gaagaaaccg
    48061 aaaagccatc ggacaagtgc agaagaatgg cggatcgcct ccgaaatcaa agggaattgg
    48121 caagatcctg aaatgaaaat cagtaacgta cttttggaag gagatgccgg gtcggggaag
    48181 acacaattag ccaaagcact atctgctgat ctgcaacttc cctatacaaa agtgacctgt
    48241 tttgcagata tggacaaatc agatgttttc ggagccttat tacctgtagt agattctaat
    48301 aaggaacaag accaagaatt actagaagct atttaccaaa cagatacact tgaagctgta
    48361 ctgacactga tccagaacca ttacggcatt gatccttttt ctgctaaaga aaaattagca
    48421 gagctagttc aaaggatcga agctgattat ccagatgcaa tccattacag ctattaccct
    48481 tctgaaatcg ttcgagcgat tgaaaaagga tatttattag agattcaaga gccgactgtc
    48541 atccgagatg cctctgttct tgtggcactt aactcagcgc tcgaaccaaa tgggatgttg
    48601 aatttaccga caggaatcat tcgtcgccat ccagattgcg tcattgtgat cactacaaat
    48661 cgaaattacc aaggaaatcg cccattaaat gaatctttaa gagatcggat gcaacatgcg
    48721 gaaaaaatgg atcttcctgc cttagaagtc atggcagaac gtgcgatggc gaaaactatg
    48781 atccaaaaac aagaacttct cttaaagatg gcagagatta tccgtttgtt ggatgaaact
    48841 gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt cttattttta ttgggcaaat
    48901 acctataagc aaggacaaga tttactccag tcgatttatc caaaagtttt gtataaatta
    48961 tccactgatt cagatgaatt ggcaattctt gaacaagctt tgtctgacag tggattgcta
    49021 ctggaacttc aggacatatt gagaaaagaa aaatggggtc atcctcaatc tgagcataca
    49081 aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa acatcactga aaatgctgaa
    49141 gaaattctgc gcttagatga aaagaaatcg ttgtctgctg aagaaccaaa tgagggagaa
    49201 gagaaacaag aagaaacaat agaaaaaaac gagattcctc ctcaaatgca aaaagaacgc
    49261 agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa caggagatga ttctcaagaa
    49321 atgtctgctt ctgacatgga agcacatgat aaggaaatca agaagcaact gaataaagaa
    49381 gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag gtctcatcgt tcaccgacca
    49441 gctttccaag gaaaaacgcc ggaagcacgt gagctacaca tgcaagtaat gccaatagta
    49501 gaatcactta gccggcaaat tttagagtta ctggataatg aacaaacaga gacgtaccaa
    49561 aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag tagcatatgg tgatttgaga
    49621 aattttgaca aaaagaaccc gccacatgag caaccttctt tagcggtagc tgtacggatc
    49681 gatgaatctg ggtcaatgat acgggatgat cggatcgaag cggcaaaaaa agcagcgatt
    49741 gccattgccg aattttctaa gaaagtcgat atcccattgt tgatttacgg agatacagca
    49801 gaccgttcgt caagagaaaa gacttctttg ttttcttata aagaatttga agacggattt
    49861 caatggttaa atgaaaaatt agtcactatg aaaccaaggc aaaacaatcg tgatggtgct
    49921 gcacttcgtt tagcagcaga aaaattaaac agacaatccg caacaaccaa actgttgctt
    49981 aatattagtg acggtcagcc aaaagcgttg ccggattata caggcaaaaa agcaaaagaa
    50041 gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa tcctatttgt ctccgcagct
    50101 attggccaag acaaagaaga aatcaagtcg atctacggtg aaagtcgttt tgtagatatt
    50161 actgacttga atgaatttcc caagcaaatg attcaactga ttgcccgcta tctatgataa
    50221 aataaagctc aacgaacgca cttagcagat tcgttgagct tttcgtttct agttaccggt
    50281 aacaaggata cttctaaaat agaatcggtt ataatcaaga gggaccgtta aaggagtgaa
    50341 acgatatgat acaagaacaa aaatggtgga aagaagcagt aggctatcaa atctatcctg
    50401 ctagtttcaa agatagcaat ggcgatggtc aaggagacat caatgggatc cgtgaaaaac
    50461 ttaattattt gaaagatctt ggtatcggct ttatctggat caatccgatc tatcaatctc
    50521 catttgtcga taatggttat gacgtaagtg actatcaaga tatagaagag agattcggaa
    50581 cgatggaaga ttttgattta ttgctaaaag aagcacatga attagggatc aaagtgatta
    50641 tggatcttgt catcaaccat agttcggatc agcatcaatg gtttgaagaa tcgaaaaaat
    50701 ctaaagaaag cccgtatcgc gattattaca tttgggtcga cggggtagat ggcaaagaac
    50761 cgaataattg gacatcgatt ttcggcggtt ctgcttggga gtattctcat gaaactggac
    50821 agtattatct gcatgttttt gcaaaagaac aaccagactt aaattgggaa agcgagaaat
    50881 tgaaagaaga actattcaat atgatccgat ggtggttgga taaagggata gatggatttc
    50941 gtttagatgc gatttctcat gtaaaaaaag atgaatattc agtaaaagcc acagagaacc
    51001 ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca tttgaccgat ttgaaacatg
    51061 tttttgaaga gtacgatatc atgactgtcg gagaagcaag tggtgtcact gcagaagaag
    51121 gaccacaatg ggtagggaaa gacggttatt tcgatatgat attcgaattt gatcatatcc
    51181 atatctggca gcaagaaaaa gaagggcaat tagatgtcct taaattgaaa cttgcattaa
    51241 gcgcttggca aacttcattg gatggtatcg gatggaacgc tttatatatg gaaaaccatg
    51301 atgttcctcg tgccgtttca gtgtttggag atactcgtcc cgatttttgg gctatgtctg
    51361 caaaagcaat cgctatgatg tatttctttt tgcaaggtac gccatttatt tatcaaggac
    51421 aggaaatcgg aatgacgaat atgccctttg aatcgatcga tcaagttgat gcagtagatt
    51481 cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac aagagaagaa gcattggata
    51541 tcattcgtga aacaacgaga gacaacagca ggacacccat gcaatggact tcggaacaat
    51601 atgctggttt ttctacacat gaaccttggc tgatcactaa tccaaatacc aaaacgatca
    51661 atgtagaaca acaagaatat gaaccagagt cggtcctgca attctataag aacatgatcc
    51721 gcattagaca aaccaataaa ggtctgatat acggttctta taaagaatat cttcatgaac
    51781 atccacaact ttatgtctat gaaagatatt tagaagatga acaatatctt attatggtaa
    51841 acttaacaga atcacttgct gactatgaac ttccaaaaga agcagatcag tcctggacat
    51901 tactcttgtc aaattcctct agcggagaat ttgaagctaa aggcatacta gcaccttatg
    51961 aagctcggtt atataaaaca aacaagtaaa acttcctcgt taaagttcat tagttggact
    52021 ttgcgaggaa gtttttcaga ttgctctgtc aattactcat ttcaataatt cgctctagga
    52081 ccaccgatat atttaggacg agagcgaagg gctgtcacgt gagcttgtcc aagggttttc
    52141 aaacgaggtc tgacaggtac ttggacatgt ttgatatgca taccgattga tgtgtcaccg
    52201 atatcgatac cggcttgtcc aactacgtgt tccacttcga caggatcatt gaactggtca
    52261 aaagcagcaa cagaacaagc acctccagca tgtagtgcag gaacaacaga gacaatttcg
    52321 aaattttttg cttctgccac tgttcgttct actactaatg ctcggttcaa atgttcacaa
    52381 ccttggacag ctagcgcaat ttctttagga tccaacaact cttttaacgt acggatgatc
    52441 cattgaccaa cttccgcact ggaatttttt ccaatatgtc ctccaacgac ttcgcttgtg
    52501 ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg cctcagccaa tatgtcatta
    52561 actatttcag tcaattggtc ttttaattct ttttctgaca acttcatttt ctaaaacctt
    52621 ctttttcaat taatattgct tccgtgcttg aatacgttga agacttgcag ttataggaaa
    52681 ggcaagtccc attccaatca gattttggaa gatgtttcct ggtatagaag ccactccagc
    52741 cggccaatca tataaaatcc aaccggcgat accatagccg atgatcataa ctaagcttcc
    52801 taataaaagc cctaatcccc acatacgagt agaagatttg ctgaaagaac cagcaacata
    52861 tccttgtaac ccgtggatca ctaatgaaaa aactgcccac tgcggatatc ctgaaatcaa
    52921 atcgatcaat agaccactgg aagcaccaac aactaatcca cccgttggac cataaagaaa
    52981 agcagcggta tagatcccgg cctcgcataa tgtcacaaat ccattcgtag ccggtacagg
    53041 gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc agcatagcat agatcgttaa
    53101 ttgcttagtt ttcatttctc attcctcctt tttgaatacc gccagaccag acacttccgg
    53161 tatgatcgtt catcagcacg ccattagaaa tacaatgggc aacaaatttt ttactttctg
    53221 ctactgcttt ggtaaaatct gcccctaaca caagattcgc tgttattgca gaagaaaagg
    53281 cacaaccggc cccgtttact gttgtagtgt cgattttttt atgagaaaat acctcataat
    53341 gatcaattga atagagaaga tccacagctt catctccagc catccttgtg ccgcctttga
    53401 tcacaatgtt ttttgttcct gaagcagtga gttctttagc gagatggatc atttctgtta
    53461 acgaagaggg aagagcttga ccagaaagta aagcagcttc tgccaaatta ggcgtaatca
    53521 tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat atacaattga ttgatcacag
    53581 cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat tgggatattt tgttttgttt
    53641 ttatgaagtc tcgaacaacc atgattgctt cacttgaatt caataaccca attttgatac
    53701 catctaatga agtcatttta tcaatcgaaa ctaactgtcg tttaagcaat tccaaagata
    53761 tagattccag cataaatcgc tcattaatat ctagtgcccc tatagaagtc aaggcgctta
    53821 gtccgaatag accaaaattc tcaaaagttt ttaaatctgt ttggattcct ccaccagcaa
    53881 atggatcaga accgccaatt gtcaagattc ttttcataac attttcctcc atgaataaga
    53941 gtataacgaa attttcaaaa aagaaaaaca gccaattggc atatttttat ctcatccaat
    54001 ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt tctttcctta aatagttagg
    54061 acttttgtga ttttaattct ttctcgattt gaagccgttg cactttcatt gtagctgttc
    54121 gcgggattgc atcaaaatca cggatgatag ggacgttcaa gcgaggaagt tcatggactt
    54181 gctcccacca tcgattccaa tccatttctt tatcaggagc taaagcaata atcggttgtg
    54241 gagaatgatt tttatccctt acaatcacta cttctgccaa aaaatctaac gaatctaata
    54301 aaaagtcttc aatagctaat gtactattga tcgtttcgat caaatccact tgccgatctt
    54361 tcaaaaatag gtggccatgc ttatccataa aaccgtaatc tccgctatcc caccaatcgc
    54421 cataaacatt ttcctggaac cgtgcatctt ccttgtaata agtcaaagcc cgtcctttag
    54481 acagtaattg tatatgacca tctgtgtttt caggtaggac attcccaact gaatctgtaa
    54541 tccgagcgct tgtcatgtct tccagtccaa cgcccatatc tcttgcatca cttgttttca
    54601 aactctctag tgtatgagcc ttaagaatca ttggtccgca ttcactttgg ccatatactt
    54661 gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa tgtcttcatt gtctggttgt
    54721 ttatagcatc aaaagtggag tgatagtatc ggatacccga aaaagcttct ggatgctcct
    54781 tagcagtaaa acgccactga acaaagttat tcggatgggt ctctagtgca attggccggt
    54841 gagcttccaa catatgaaca acttttgaac tttgtgcgtt tgctaaaggc atcataggaa
    54901 atcccatgga catcaaagaa gagataccaa tattgaatct ggaatgcact ggagagatgt
    54961 gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac tgttttttgc catttcgtcc
    55021 gccatcccat agaatgagca gaatgacaaa taagtttagg gataccagtt gtaccagaag
    55081 tatgggtcat ataagaaatt tcatttttgt ctaaaaatga ttgagaaaca gacaaagcag
    55141 gttgttccat taaataaaga ataggaatac ccgtggtgct agttaatttt aatatcctaa
    55201 agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa cactgtaagc
    55261 cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt tgaaattttg
    55321 acaacctaat ttttctaagg aagaaaaaac agtctcaact gtttttcttt gttttccaag
    55381 taagctgtgg tctagttttt ctgatttatc catgttttta cgtgg
//
LOCUS       DACDNO010000014        49403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDNO010000014 DACDNO010000000
VERSION     DACDNO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.014"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            200..2734
                     /gene="secA"
                     /locus_tag="IWI22_000857"
     CDS             200..2734
                     /gene="secA"
                     /locus_tag="IWI22_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9168264.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2860..3961
                     /gene="prfB"
                     /locus_tag="IWI22_000858"
     CDS             join(2860..2922,2924..3961)
                     /gene="prfB"
                     /locus_tag="IWI22_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9168265.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4066..4752
                     /gene="ftsE"
                     /locus_tag="IWI22_000859"
     CDS             4066..4752
                     /gene="ftsE"
                     /locus_tag="IWI22_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9168266.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4736..5629
                     /locus_tag="IWI22_000860"
     CDS             4736..5629
                     /locus_tag="IWI22_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9168267.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5781..7229
                     /gene="cls"
                     /locus_tag="IWI22_000861"
     CDS             5781..7229
                     /gene="cls"
                     /locus_tag="IWI22_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9168268.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7566..8423
                     /locus_tag="IWI22_000862"
     CDS             7566..8423
                     /locus_tag="IWI22_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9168269.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8538..9458
                     /gene="pstC"
                     /locus_tag="IWI22_000863"
     CDS             8538..9458
                     /gene="pstC"
                     /locus_tag="IWI22_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9168270.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9458..10342
                     /gene="pstA"
                     /locus_tag="IWI22_000864"
     CDS             9458..10342
                     /gene="pstA"
                     /locus_tag="IWI22_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9168271.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10357..11163
                     /gene="pstB"
                     /locus_tag="IWI22_000865"
     CDS             10357..11163
                     /gene="pstB"
                     /locus_tag="IWI22_000865"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9168272.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11179..11937
                     /locus_tag="IWI22_000866"
     CDS             11179..11937
                     /locus_tag="IWI22_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9168273.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11950..12627
                     /gene="phoU"
                     /locus_tag="IWI22_000867"
     CDS             11950..12627
                     /gene="phoU"
                     /locus_tag="IWI22_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9168274.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12865..13896
                     /locus_tag="IWI22_000868"
     CDS             12865..13896
                     /locus_tag="IWI22_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9168275.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13893..14660
                     /locus_tag="IWI22_000869"
     CDS             13893..14660
                     /locus_tag="IWI22_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9168276.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14760..16328
                     /gene="liaX"
                     /locus_tag="IWI22_000870"
     CDS             14760..16328
                     /gene="liaX"
                     /locus_tag="IWI22_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9168277.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16628..16945
                     /locus_tag="IWI22_000871"
     CDS             16628..16945
                     /locus_tag="IWI22_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9168278.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            16945..17295
                     /locus_tag="IWI22_000872"
     CDS             16945..17295
                     /locus_tag="IWI22_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9168279.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17462..18400
                     /locus_tag="IWI22_000873"
     CDS             17462..18400
                     /locus_tag="IWI22_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9168280.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18415..19245
                     /locus_tag="IWI22_000874"
     CDS             18415..19245
                     /locus_tag="IWI22_000874"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9168281.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19289..20314
                     /locus_tag="IWI22_000875"
     CDS             19289..20314
                     /locus_tag="IWI22_000875"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9168282.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20329..21267
                     /gene="galU"
                     /locus_tag="IWI22_000876"
     CDS             20329..21267
                     /gene="galU"
                     /locus_tag="IWI22_000876"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9168283.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21392..21832
                     /locus_tag="IWI22_000877"
     CDS             21392..21832
                     /locus_tag="IWI22_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9168284.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21835..22437
                     /locus_tag="IWI22_000878"
     CDS             21835..22437
                     /locus_tag="IWI22_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9168285.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22496..23602)
                     /locus_tag="IWI22_000879"
     CDS             complement(22496..23602)
                     /locus_tag="IWI22_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9168286.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23812..24813
                     /gene="ccpA"
                     /locus_tag="IWI22_000880"
     CDS             23812..24813
                     /gene="ccpA"
                     /locus_tag="IWI22_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9168287.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25150..27495)
                     /locus_tag="IWI22_000881"
     CDS             complement(25150..27495)
                     /locus_tag="IWI22_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9168288.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27774..28676
                     /locus_tag="IWI22_000882"
     CDS             27774..28676
                     /locus_tag="IWI22_000882"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9168289.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28889..30664
                     /locus_tag="IWI22_000883"
     CDS             28889..30664
                     /locus_tag="IWI22_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9168290.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30730..32316)
                     /locus_tag="IWI22_000884"
     CDS             complement(30730..32316)
                     /locus_tag="IWI22_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9168291.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="IWI22_000885"
     CDS             32566..32790
                     /locus_tag="IWI22_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9168292.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="IWI22_000886"
     CDS             32932..34683
                     /locus_tag="IWI22_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9168293.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="IWI22_000887"
     CDS             34683..36467
                     /locus_tag="IWI22_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9168294.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="IWI22_000888"
     CDS             36793..37644
                     /locus_tag="IWI22_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9168295.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="IWI22_000889"
     CDS             37698..39449
                     /locus_tag="IWI22_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9168296.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="IWI22_000890"
     CDS             39453..39623
                     /locus_tag="IWI22_000890"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168297.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="IWI22_000891"
     CDS             complement(39663..40565)
                     /locus_tag="IWI22_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9168298.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="IWI22_000892"
     CDS             40796..42199
                     /locus_tag="IWI22_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9168299.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /gene="ascB"
                     /locus_tag="IWI22_000893"
     CDS             42199..43632
                     /gene="ascB"
                     /locus_tag="IWI22_000893"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9168300.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="IWI22_000894"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="IWI22_000894"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9168301.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="IWI22_000895"
     CDS             44917..46644
                     /locus_tag="IWI22_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9168302.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46716..47009
                     /locus_tag="IWI22_000896"
     CDS             46716..47009
                     /locus_tag="IWI22_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9168303.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="IWI22_000897"
     CDS             complement(47101..47961)
                     /locus_tag="IWI22_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9168304.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="IWI22_000898"
     CDS             48053..48481
                     /locus_tag="IWI22_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9168305.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /locus_tag="IWI22_000899"
     CDS             48643..48819
                     /locus_tag="IWI22_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9168306.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="IWI22_000900"
     CDS             48846..49292
                     /locus_tag="IWI22_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9168307.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        16012 a         8660 c        10596 g        14135 t
ORIGIN      
        1 gaaaacttgc agcagctact gacagctgcc gcaagttttt ttgagtttcc tcctctttta
       61 gatgaaatat gaataaatta tgaagttctt actttaatac gcttaagata tttcaattat
      121 ctataactaa tgataaaatg aaaagtaaag tctacatagt ctggataata cacagaagga
      181 aaaggagatt acacaacgca tggccaattt gattcgttca cttatcgaaa acgataagaa
      241 agaactaaaa cgtttagata gcattgcaaa aaaagttgaa gcatacgctg accaaatggc
      301 agcattaact gatgaacaat tacaggcaaa aactgctgaa ttcaagggca gataccaaaa
      361 aggagaaaca ttagatcaat tacttccgga agcttttgca gtcgtccgag aagcagcaaa
      421 acgtgtattg gggttatatc cacaccacgt acaattgatg ggtggaatcg tgcttcacga
      481 tggaaatatc cctgaaatgc gtactggtga aggtaaaacc ttgaccgcaa cgatgccagt
      541 atatctaaat gctctgactg gcgaaggggt acacgtagtt accgtcaatg aatatttagc
      601 aacccgtgac tcgacggaaa tgggcgaact ttataatttc ttaggattaa cagtggggtt
      661 gaatatcaac tcaaaatctt cggatgaaaa acgcgaggct tataattgtg atatcactta
      721 tagtacgaat aatgaattag gatttgacta cttgagagac aatatggtcg tttaccgcca
      781 tcaaatggta caacgtccgc tgaattatgc cattgtcgat gaagttgact caatcttgat
      841 tgatgaagcg agaacgccgc tgatcatttc agggcaagca gaaaaatcca cagctttata
      901 tacaagagca gacaacttcg tgaaacgttt gaaagaagaa gaagattata aaatcgatgt
      961 acaatcaaaa acaatcagtt taaccgaagc agggatcgaa aaagcagaag aaacattcgg
     1021 tttggataac ttatacgaca ttgaaaatac tgcattgacc catcacttgg atcaagcatt
     1081 gagagccaac tatatcatgc ttttagatat cgattatgtt gtccaagacg gaaaagtact
     1141 gattgttgac cagttcactg gtcggatcat ggatggacgc cgttattcag atggacttca
     1201 ccaagcaatc gaagctaaag aaggcgtaga gatcgaagac gaaacgaaaa ccatggcgac
     1261 gatcactttc cagaactatt tccgtatgta caagaaactt gcgggaatga ctggtacagc
     1321 taaaacagaa gaagaagaat tcagagaaat ttataacatc caagtatacc agattccgac
     1381 aaatcgtccg gttatccgtg atgatcgtcc agatttgctt tacccaacat tacaaagcaa
     1441 gttccacgct gtcgtgcaag atatcaaaga tcgctaccgc aaaggtcaac ctgtgttagt
     1501 cggaactgtt gcggttgaaa catctgagtt attatcagat atgctgaatc aagaacgtat
     1561 tcctcatgaa gtgctgaatg cgaaaaatca ctttaaagaa gcagagatca tcatgaatgc
     1621 tggtcaaaaa ggtgctgtga cgattgctac caacatggct ggtcggggaa cagatatcaa
     1681 gttaggactt ggggtaagag aacttggtgg tctagcagtt atcgggacag aacgacatga
     1741 atctcgtcgt atcgataacc agcttcgagg acgtgcagga cgtcaaggag accctggtat
     1801 gtcacagttc tacttgtcat tggaagatga tttgatgaaa cgattcggtt cagaacgaat
     1861 caaatctttc ttagatcgga tgaagatcgg tgaggaagat gctgttatcc aaagcaagat
     1921 gctgacgaaa caagtagaat ctgcacaaaa acgtgtagaa ggaaataact atgacacacg
     1981 taaaaacgtc ttacaatacg atgatgtgat gcgtgaacaa cgtgaagtaa tctataaaca
     2041 gcgacaagaa gtaatcatgg aagaaaaaag cttatccaag ccactaatga acatggtcaa
     2101 acggacaatc agtcgtgtag tagatgcgca tacacaatta gagaaagaaa attggaattt
     2161 ggaaagtatc atcgattttg ccggcagtac tcttgtccat gaagatagta ttactcttgc
     2221 agatatcgaa ggaaaaacag ctcaggagat caaagagtat ctaaacgaac gagcacaaga
     2281 agtatttaat accaaagcgg ctcaactaaa tggtccagag caactgttgg aatttgaaaa
     2341 agtcgtgatc ttgcgtgttg tggactctaa atggacagac catattgatg caatggatca
     2401 attacgtcag tcaatcggct tacgtgctta tggacagaac aatccattag tcgaatatca
     2461 aacagaaggc tataagatgt ttgaagatat ggtaggcgcc atcgagtatg aagtcactcg
     2521 aatctttatg aaagctgaga tccgtcaaaa cgtacagcgt gaacaggttt ctcaaggaac
     2581 agcttctcac tcagaagacg gagatgccaa agaaaatagt aatactagcg cgaaaaaaca
     2641 gccaatccat gtgaacaagg tcggaagaaa tgatccttgt ccttgtggaa gcgggaaaaa
     2701 atataagaac tgtcatggaa aaaatgcaaa ctaatttctg acagcaggat tggtgagaca
     2761 aattcttttt ttaagaattc gtctcacctt ctttctgtta tttgctgatt ctgttagact
     2821 agatataatg aaaaaagtat agtgaaagaa ggaaaaaaca tggaaatatc cgaaattcga
     2881 aatctcctga cagaaatgca ggaaaaggtc acaagcttca ggaggtctct ttgacttaga
     2941 ccagctggaa gaagacattg cggaagcaga agcaaaaatg gctgaacctg gtttttggga
     3001 tgatagcgaa gctgctcaaa aagtgatcag tgaaaacaat gcaaataaag aaacttatga
     3061 tcaattccat tctcttgctg aagaactgga agaaatcgaa ttgatggtgg agatgttgca
     3121 ggaagaacct gatgcagcca tgcaagaaga agcagaaaca cgtatcaatg cgctagatga
     3181 aaaaatgaag acatatgaat tatctatgtt gttagatggt ccttatgatc gaaacaacgc
     3241 catcgttgaa ttgcatccag gggctggcgg aacggagtcc caagattggg gcagtatgct
     3301 tttacgtatg tatacacgct gggcagagca gcatgggtat caagtagaga cactagatta
     3361 tcaagcaggt gatgaagcag gaatcaagag tgtgacccta ctgatcaaag gacacaacgc
     3421 atttggttat ctgaaatcag aaaaaggtgt tcatcgcctt gtacgtatct cgccttttga
     3481 ctcagcgaaa cgacgtcata cttctttttg ttcggtagat gtgatgccag agcttgatga
     3541 aacgatcgat atagaaatca atccagatga tttgaaaatc gatacgtacc gagcaagtgg
     3601 tgcaggtggt cagcacatca ataaaacaga atctgctgta cggatcactc acttgcctac
     3661 aggaacagta gtagctagtc aggcacaacg ttcacaattg aaaaaccgtg agcaagcgat
     3721 ggggatgtta aaagctaagc tttatcaatt agaagtggaa aagaaagaac aagaagcagc
     3781 tgcacttcga ggagaacaaa aagaaatcgg ctggggttca cagatccgtt cttatgtttt
     3841 ccatccatat tctatggtaa aagaccatcg gacaaacgta gagacaggaa atgtccaagc
     3901 agttatggac ggtgaccttg atttatttat tgacggctat ttaaaaatgc atctacaata
     3961 agaacccatt gttaaattga aataaaattg taagattttg caagcatgtt gaaacattgc
     4021 catgctataa tagctaaaga ctgaaaaaga tatggagaga acgccatgat tgaaatgaag
     4081 gatgtaatga agaagtactc aaatggtacg accgctatcc gcaatctctc aattgtcatc
     4141 gaacaaggag agtttgtcta tgtggttggt ccgtctggag ccggtaaatc tacattcatc
     4201 aaattgatgt atcgagaaga aaaagcttca aaaggaactt tgaacgtcgc aggtcatgat
     4261 ctaatagcaa tcaaaaaccg cgaagttcct tatctacgca gagaaatcgg cgtggtgttc
     4321 caagactata aattattacc aagaaaaaca gtatatgaaa atgttgcgta cgctatgcaa
     4381 gttatcggca gaaaacctcg ggatatcaaa aaacgagtaa tggaagtgct ggaccttgtt
     4441 ggtttgaaac acaaagttcg agtctttcct agcgaattat ccggtgggga acagcaacgt
     4501 gtctctattg ctcgggcaat agtgaatacg cctaaagtac ttatcgcgga tgaaccgaca
     4561 ggaaatctgg atccagaaaa ctcatgggaa atcatgaaat tattagatcg gatcaatgcc
     4621 caaggaacta ctgtcgtgat ggctacacat aacagtacga tcgtaaatac gattcggcac
     4681 cgagtaattg ctatcgaaaa cggccgcatt attcgagatc aagcggaagg ggaatatgga
     4741 tacgatgatt agaacctttt tccgccattt attagaaagt atcaagagtt tgaaacgtaa
     4801 cggctggatg acagttgctt cagtcagtgc ggtaacgatc acattgactt tagtcggtgt
     4861 attcatggca gttatcatga acgcaactaa attagctcaa gatatagaag gaaacgttga
     4921 tgtctctgta ttcgtagata tcggtacaaa agacgaagag atgaaaaagc tagaaaaaga
     4981 attgaatgac ttagatcatg ttaaaaaagt tgaattttca agcaaagaac aggaattgaa
     5041 aaaaatccaa aacgaaatgg gtgattcctg gaaattgttt gaaggtgaca ataacccgtt
     5101 atatgatgtg tatgtagtaa gtgctactga tccttcttat acaaaacaag tagcagaaga
     5161 agcagcagat ttgaagaatg tttctcgttc tgattacggt ggagcttcct cagatcggat
     5221 tttccaaatc gctggtgcag ttcggacatg gggattagtt gcagcggcat tgctgctgtt
     5281 cgtagcgatg tttctcattt cgaacacgat tcggatcacg attttgtcta gacagcgtga
     5341 aattcaaatc atgcgtttag tcggtgcaaa aaatggatat attcgctggc cgtttttcct
     5401 tgaaggtggc tggatcggtc tgatcggtgc gattcttccg attttgatca tcacatttgg
     5461 ttatgcatgg tttttccaat tgatcaatcc atcattatta cgttcccatt attcattgat
     5521 tcatccacaa aatatcgttt ggaaaatcaa tcttttaatg gtcggcattg gtgtgatcat
     5581 tggttcactt ggctcgatca tttctatgag acgtttcttg aaattctaat accacaatat
     5641 aaataaaatg agaaagttgg acagtttcgt tccagctttc tttttttatt ggctaggaga
     5701 atagaaggaa gcggcttttt tccctttact ttaaaaaaca acttgggtat tcttaagttg
     5761 gtagaagaag aggtgaagcc gtgaaaatta tcgttgacaa tttttttacg atccttttga
     5821 ttatgaatat tttgctttcc tttatcatcg tttttcgaga aagaaaggaa actgctcaaa
     5881 catgggcatg ggtgctcgtt ttgatgttca ttccagttgt aggatttatt ttatatattt
     5941 ttttaggaag agggatttcc aaagagaaaa tctttgattt gaagatccaa gggaaaatcg
     6001 gtaagaacct ggaaattgaa gaagacaaac aggcattgat gcgagggctt tatcctcatc
     6061 cgcctactgg acaagtagat gtgaaacaat tgatctatat gctcacggta tttgaaagta
     6121 cgctctatac gactaaaaat gaaatcactt tatttacaga tggtcgtgaa aaattcgatg
     6181 cgctgattga agatatcaag caggcagaag accatatcca cttccagtat tatatttatc
     6241 gcagtgatgc attaggtgaa gaagttcgag atgcattgac agaggcggca agacgagggg
     6301 taaaagtcag agtccttctt gatgcatggg gttccacaca agtttcttcc agcttttttc
     6361 agaatttgaa aaaagcaggc ggagaagtcg catttttctt tcctctattt gttccgtata
     6421 ttaatcctcg gatcaattat cgcaatcatc gtaaaatcgt agttattgat gggaaaatcg
     6481 gatatacagg tgggttcaat gttggaaatg aatatctagg attggtaaag aaatttggtt
     6541 attggcgaga caatcatttg cgtatttacg gagaagcggt gtataccttg caaaaccgtt
     6601 ttttgatgga ctggaattct cagcatacaa aagaagtagg ctattctccc aaattttttc
     6661 cgtccattca ctctactggt gatatagctg ttcaaatcgt gacaagcgga ccagataccg
     6721 aacatgaaca aatcaaaatg acttatttga aaatgatttc gttagccaaa cgggagatat
     6781 tgatccaaac gccttattac ataccggatg gatcgattca tgaagcactg aagttggctc
     6841 ttctttcagg cgtaaaagtt catatccaaa tacctaacaa accagatcat cttctcgttt
     6901 actgggcaac gtattctttc gctgcagagc taatcgagta tggagcaagg atcgaaacat
     6961 atgagaatgg atttattcat gcaaagacga tgattatcga tggtggcatt gtttcagttg
     7021 gctcagcaaa tatcgatgtt cgttcatttc gcttggactt tgaggtcaat acactggttt
     7081 atgatgaacg aatggcttca cgtgtaagaa aagctttttt tgaagactcg aaaatttcta
     7141 cgcatctcac aaaagaaatg tatgaaaacc gaggaataat catcaagatg aaagaaggat
     7201 tagcaagatt gatttcgccg ctcctttaaa acataaaaag gacatgagaa ccatcaacag
     7261 atggagatct cgtgtccttt tctaacgggg ccatttctga gagtacgtca gaaatggaga
     7321 aagcgtctcg catgagcgaa attaactttg gggtatctct tgttttttgg ggaaagcaag
     7381 aaataagcta atatatgcta ttaaaagaga cacatttcaa agccagtata gcatagaaac
     7441 ttttaatgag atagtttttt ttacaattat gagaatttat tttgttattt aatgattttg
     7501 attcgttttc atcaagtaat gaaaatgata aaataaacat actttaggat taaaggagat
     7561 ttctaatgaa aaaacttaaa ttattcttac caataatagg attagctatt cttttatcag
     7621 gctgttcaaa gtggattgat cgaggagaat ccattacggc tgttggctct tctgctttac
     7681 agcctttagt agaaacagtt gctgaaactt accagacaga aaatcctgga aaattcatca
     7741 atgtacaagg gggcggaagc ggaactggtt tgtctcaagt acagtcagga gcagtcgaca
     7801 tcggaaactc tgatttgttt gcggaagaaa aatcggggat cgatgcctca gcattagtcg
     7861 atcatcgagt agcagttgtt gggatcactc ctattgtgaa caaggatatt ggcgtaaaag
     7921 acatcagtat ggaaaacctg aaaaaactat ttacaggcga gatcaaaaac tggagtgaag
     7981 tcggtggaaa agatcagcca gtagttatat tgaaccgcgc ttcaggaagc ggaacccgta
     8041 gcacgtttga aaaatgggta ttggacggag aaacagctgt tcaggcacaa gaacaagatt
     8101 caagcggaat ggtgcgccag atcgttgccg acacacccgg tgcaatcagc tatgttgctt
     8161 tttcttatgt ttccgatgat gtagatacgt tgaatgttaa cggtgtagaa ccgacagatg
     8221 aaaatgtgac aacgaacaaa tggacgattt gggcatatga gcatatgtac acaagagatc
     8281 atccaaaaaa actgacaaaa gaatttttag actatatctt atctgatgaa gtacaagata
     8341 acatcgttgg agaactaggt tatatccctg tttctcatat gaaagtcgaa cgtgattggc
     8401 aaggaaatat cgttaaagag taatctttac acaaaattta caaacttagc taaactttta
     8461 catgttatta aaaccaaatt gatgttcaat ttgtacactg agtgagtatg ttaaacctga
     8521 ttaaggggga gtcatctgtg gaagatgtga aaaaagtatt attaacaaaa tcaaaacgtt
     8581 caaaaatgga acagcgtgga aaattcatca gttttttatg tatcgcgctg atcgtgttgg
     8641 ttgtattatc tattttttat tttgtagcta gtaaaggatt ggccaccttt tttactgata
     8701 agatcaatgt atttgatttt ctgtttggta cagattggaa tccaagccaa atcggagcaa
     8761 atgggaaacc aatggtaggg gcattgccta tgatcacagg ttcgtttatt gtgacctttt
     8821 tatctgctat cgttgccacg ccatttgcca ttggtgcagc agtattcatg acggagatct
     8881 ctcctaaaaa cggaacgaag atcttacagc cggtcatcga gctgcttgta ggtattcctt
     8941 ctgtcgttta tggttttatc ggtttatcag ttatcgtgcc ttttgtcaga agtatttttg
     9001 gcgggactgg gtttggtatc ttagctggta catttgtgtt gttcgttatg atcttgccga
     9061 ctgtcacaac gatgactgtt gatgcattaa aggcagtgcc acggcactat cgggaagctt
     9121 cattagcgct tggtgccaca agatggcaaa caatctacaa agtcgtgcta cgagcagccg
     9181 tgcccgggat tttaacagca gtcgtttttg gtatggctcg tgcgtttggt gaagccttag
     9241 ctatccaaat ggtcatcggt aatgcagcat tgatgccaac aggtcttact actcctgctt
     9301 ctacattgac gtccatctta acaatgggaa tcggaaatac aatcatggga acagttgaaa
     9361 ataatgtgtt atggtcatta gcgctgatct tgctattgat gtcattattc tttaatatcg
     9421 tgatccgtat gattgggaag aaaggagagc tgaaataatg aacgcaaaac gctcagataa
     9481 aattgctaca ggtgttttat atatagtttc ggggatcatc gtattgattt tagcagcttt
     9541 actactttat atcttagttc ggggaatacc gcacatttca tggtccttct tgaccgaacc
     9601 ttccaaagcc taccaagaag gcggtggaat cgggatccag ctatttaact cgatttattt
     9661 gcttttgatc acgatgatca tcagttttcc tatttcttta ggtgcgggta tctatctttc
     9721 agaatatgct ggaaaaaact ggctaacaga tgtgattcgc acgtcgattg aaatcttgag
     9781 ctcgttgcct tctgtcgttg tcggtttatt cggtttcttg gttttcgtta ttcaattcca
     9841 gtatggtttc tcgattattt ctggtgcatt ggctttgact gtattcaact tgcctctttt
     9901 gactagaaca gttgaggagt ctttacaagc ggtccattac acacaacgag aagcaggatt
     9961 ggccttaggt ttgtcacgct gggaaacagt gataaaagtg gtggttccag aagcgacacc
    10021 agggattttg actggtgtga tcctaagttc tggaagaatt tttggtgaag cagctgcatt
    10081 gatctatacg gctggacaaa gtgcacctgc gttagatttt acgaactgga atccattaag
    10141 tgtttcaagc ccgatcagca ttttccgtca agctgaaaca ttagctgtcc atatttggaa
    10201 gatcaataca gaaggcacga tgcctgatgg tgtttctgtt tcggctggcg cttctgccgt
    10261 gttgattatt gctgttttac tgttcaactt cggcgctcgt gcgattggca aacgattata
    10321 ccgaaaaatg acttcagcct aaggagaaga agcaaaatga aaaaatataa ctggaatgaa
    10381 acaaatgtca tcaccatgcc aaaagagaaa gaagtagcac tatatacgga tgatctacat
    10441 gtctggtatg gcgacaatga agcaatcaaa ggagtggatc tggaatttga aaaaaataag
    10501 atcactgcac tgattggtcc ttccggctgc ggaaaatcta cgtatcttcg ttcgttgaat
    10561 cgaatgaatg atgggatcgc taatacgaaa atcactggga aaatcatgta tcaaggcgta
    10621 gatattaatg cgcctcaagt ggatgtttat gagatgcgca aacggattgg catggttttt
    10681 caacgtccta atccgttcag caagtcgatt tatgacaata ttacatttgc tttgaaacag
    10741 cacggcgaaa aaaacaaaaa gaaattagat gaaatcgttg aaacaagctt gaaacaagct
    10801 gccttgtggg accaagtaaa agacagcttg gacaaaagtg ccttggcttt atctggcggg
    10861 caacagcaac gtttatgtat tgctcgtgcg atcgcgatga aacctgatat cttgctgcta
    10921 gacgagccgg caagtgcttt ggaccctatt tcgacaggaa cggttgaaga aacacttgta
    10981 aacttgaaag aagactatac gatcatcatc gtgacgcata acatgcaaca agcagctcgt
    11041 atcagcgact atactgcctt tttctattta ggaaaagtaa tggaatatga caaaactaac
    11101 aagatattta ccagaccaaa aattcaggca accgaagatt atgtgtccgg tcattttggt
    11161 taagacggag gcctcaacat gacaaaagag attatttcat ctaaagatct tcatctgtat
    11221 tatggaaaga aagaagcact aaaagggatc gatctaagct tcaatcaagg ggaaatcact
    11281 gcgatgatcg gtccttctgg ttgcggaaaa tccacttatc tgcgttcttt gaaccggatg
    11341 aatgacctga ttccgaatgt aacgatcacc ggaagtgttt tatataaaga caaagacatc
    11401 tatggtccaa aaacagatgt tgttgaacta agaaaagaaa tcggcatggt attccagcag
    11461 ccaaacccat ttcctttttc tatctatgaa aatgtcatct atggattgaa attaaaaggg
    11521 gaaaaagata aaaaagtcct tgatcaagta gttgaagaaa gcctgaaggc agcgtctgtt
    11581 tgggatgatg tgaaagacaa attgcataaa agtgctttat ctttatccgg tgggcagcag
    11641 cagcgtgtat gtatcgcacg tgttttggca gttaatccgg aaatcatctt attggatgaa
    11701 ccaacaagtg cgcttgatcc tgtttctact gggaaaatcg aaagtatgct gttagaatta
    11761 agagagcggt atacgatgat catcgtgacc cataatatgt ctcaagcttc acgtatttca
    11821 gataagacgg cattcttttt agatggtcac ttgatcgaat acaatgatac gaagaagata
    11881 ttcatgaatc cagaaaaaca agagacagaa gattatattt caggtcgatt tggataagga
    11941 ggaaacggaa tgctacgtac acaatttgaa gaagaattgc tgaatctcca taatcagttc
    12001 tacgaaatgg ggatgatggt gagcagcgcc gtgcacaagt cagttcgtgc atacatcaaa
    12061 cacgataaaa tcttagccca agaagttatc gaaaacgata taaatatcaa taacatggaa
    12121 acacgcttgg agaagaaaag ttttgaaatg atcgcattgc aacagccagt tacaactgat
    12181 ttgcgtatga tcattacggt catgaaagct agctcggatt tagaacgtat ggccgatcat
    12241 gcggtgtctg ttgctaaatc cacgattcgc gtaaaagggc agacgagaat accggaaatc
    12301 gaaaaagaga tctccgatat gtccgattat gtgaaaaaaa tggtggacaa cgtcctagtt
    12361 gcttatgtca aaacagacga aaaagacgca cgtacgatcg ccaatatgga tcaaagagtc
    12421 aatgactatt tcaatcgaat ctataatgca acaatcaaaa atatgcagga aaatcctgag
    12481 acagtcatca gtggaacaga ctacttgaat gttgccagtt atctagaacg gatcggagac
    12541 tatgtgacaa acatctgtga atggatcgtc tacttggcta cagggaaaat cactgagtta
    12601 aatacgaacc acaatgagat tttctaaact gaagctgtga ttatgccgtt tgtttaggaa
    12661 aagaggttgt gaaatcagcg gttgtgaaaa agctgttctg catcaagtaa taagaacagt
    12721 caaacaaaaa atagctcctc atatttttcg tttggctgga cttattaccg cagatgcaag
    12781 cttttgaaca ccgtttattc gggaaagggt ttgtgacaaa acatgtcaca aacccttttc
    12841 atcatttcat agaaaggata aagaatgcaa aaaaaaatta gcatcatcac tatatttttt
    12901 atttgtgtgc ttacttcatg ggttattcag catcaagccg ctttatatga agtacctgtc
    12961 atcgagatag aaaaagtacg aataaaagag gatcatcaat tgattacagg aaaactgatg
    13021 aacacggatc aaaaaggaga atcgatttat ttatcggttc cttatcaaaa aaatcaaata
    13081 gaaacagcaa atataaaagc gagacaaatg tatctagtcc aaatgcatgg agatgaatgg
    13141 cagctagtag caaaaaaaaa tgatggctgg cttttcttct tcacaagtat cttcattggc
    13201 gccatgttgc ttattggtgg aaaacagggt ctatttgctt taggaagtct catcatcaat
    13261 atcgttttac ttttactttt gttgctcatc tttactaaaa cagaagggat ttctctatta
    13321 aatatatcta tcctgttcgt cgttataggg atgattatta gcatattggc tttagatgga
    13381 tggaatagaa gcagtataat caaaatttca gctgcagtta cgagtgtttt tttagcattt
    13441 tttatttgtc ttttgacaat gaatcattgg aaagaccaag gactgcgcta tgaggaactg
    13501 aattatctga cacgaccgta tcgttctgtt tttctttcta gcgtactgat tggaacagct
    13561 ggaggaacac tcgataccgt tatcacagtc attgcgacat tagaagaatt gactaagcga
    13621 caaccagaaa taacagcgaa acagttatgg ctttcaggaa ggcaagtggg tcgagatgtg
    13681 attggctcga tgaccaatat ccttttgttc gtctatatca gtgggtcagt tccatcgctt
    13741 cttttatatt taggaaatca atggtcgttc aaagaaacga tagaacagca tttatcattg
    13801 gaatttttac gcgtcattgc tggcagctta ggaatagtat tatcgatccc tatctcggtc
    13861 ctcttttttc tgactgtaag gaggctgaaa cgatgaatgt acttattata cttgttggga
    13921 ttttagcaat cagcgtgcta cttattggcg gtacgcaagg tatgtatatt cttttaggct
    13981 tattcatcaa tttagggatc tttttcctgt tgttattcgg ttatcatcag aagtggccga
    14041 tactggcatt gtcggtcata ggatttttat tgattgctgt ggttatctta ttttttatca
    14101 acggttataa tctaaaaatg cgtgcagctt ttgtgagtat actgatcttt ttattttgtt
    14161 tcctactatt gcttcctatc actgattttt tagcaatcca aggttttaca tcggtagaac
    14221 tagaagaatt atctggctta gataagactt tagcgattga ttttagatta cttacgagat
    14281 cgttgcttct gattagtctt agtggtgctg ttttagatgc gagtgtagca atttctagtg
    14341 ggacattcga agtatatcag gcaaatccgc aattatcttt ttaccaattg cgccattcta
    14401 gtttcgctat tgctaaaaaa atattggcgt caacagtaat gacacttctt tttgcattca
    14461 tgggaagttc tttagcgttg atcctctggt ttattgattt agacattcct tttcagcaag
    14521 tgatcaatga aaaatcattt gttctagaat atacgatggc tattcttacc acattgtccg
    14581 cactacttgt tcttccttta acctgcgtga ctacagcata tctctttaca aaaaagaaag
    14641 aacaactcaa gatggagtaa aagttacaac ttaagtccta tgacattttt actgaatcag
    14701 tgcataatat ggatgtaagg aaaagcttgc aaacaatttt gcttaaggag gcaatttcca
    14761 tgaaagaaag agaacgtatt ttagatttag tgaaaaaagg aatcctttcg actgaagaag
    14821 gcttagattt attagaaagc atggcaactg aaaaagacga aaagcaaatc aaaaaagaag
    14881 cagacaaggt aactgctagt cataaagaaa aagatcaggc aagtcaattg atcgataaac
    14941 ttgaaaacgg cgaagaagaa atttctgagc cagttgatcc aaaggaaaga gaaagacaag
    15001 atcaagaaaa cttagaaaag atcttagatg aactagcaac tgaagcgaat aaaacttcgg
    15061 cgcgtctaga tgaagtaaat gctcaattcg ctgacaataa ggcagcacgc aatgagaaac
    15121 aggaagcatt gatgcagctg aatacaaaag aagaattagg cgagttgaca gaagaagaat
    15181 tagcacagcg tcagacgtta gaagcagaaa tcaaagaatt agaagctagt ggcgatacac
    15241 tgttagaaga acaattgaaa ttagaagcag aattaaaaga tatccgtaag aatcaatggt
    15301 ctgagaaaaa agaaacgttt actgataagt ttgaattgcc agatgattgg aaagatcaag
    15361 caacagatac actgaatcaa gtaggcgaaa aaatgtcaga agctggatca cagttaggca
    15421 aattcttgaa gaaaacattc caaactgttt cagaaacagt gaatgacaac atggaatgga
    15481 aagatgtcag cttacgtgtc cctgggattg cgacaacgaa atttgagcat gaattttact
    15541 atgaagctcc tgcagcaagt atccttgata tcaaggcagc aaacggaaac gtgacgttaa
    15601 aaacgtggga cagcaacgat gtgaaagttg aagctaagat caaactttac ggaaaaatgg
    15661 gtgctgaacc atttgaagca ttctctgaac ggagccagat tgaggttaat gaagatcata
    15721 tctccttcca aattccaaat aaacgtgtgc gtgcagacct tgttttctac ttacctaaac
    15781 gagtatacga ccacgcagct attaaacttc taaatggcaa catcatgatt gaaacattgg
    15841 aagctaaaga tatttataca aaatctacga atggtaacat catcgtggac caattaactg
    15901 caacgatgct ggaagtagaa ggtgtaaacg gcaatatcga tattcgaaac gggaacatct
    15961 tagattctat tatcgaaaca gtaaatggta cagtgacatt tggtgcgaca gcagaaaacc
    16021 tttctgtgtc tttggtaaat ggtgatgtac gtcttacgat taaagaagat aatttgaaaa
    16081 aagtagaagc aagttcagtg aacggaaatg tcaaagtcgc tttaccagat ggcatcggtt
    16141 tggaaggaca tgctaaaaca agtcttggaa gtatcaacag ccgtctgtcc aattacgaag
    16201 tagttcgtga gaaaaaagaa cggacaaacc aaatgttgca attccgtcgt gtttcagatg
    16261 gtgaaatcgc gcaagtgcaa ctttcaacga caacaggaag tatctattta aaagatacag
    16321 ataaataaag aggttgtgaa agagatgttc agccttgagc aataagaagc gattttgtaa
    16381 aatagcttcc cctattttac aaaattgtgc cttattgccg atagtctatc tcttgaacac
    16441 cgtttaccaa gagattatga acctgccaat tatcgccgag tcacaaggaa taatttcaga
    16501 aaaagctcgt cacgttttat gaatatggta atttgtgacc gagaattttg cagaaagaca
    16561 ccgattaaga agaaggttgt cacaacgatt ttcttcattt aaaatagtag aacagaggtg
    16621 agacatgatg aaaaaaaagt tgacaaaatc taataaaaat gttgttttga cagggacttt
    16681 agcaggaata ggagaataca tcgggattga tccgactgtt atccgtgtgc tttatgtatt
    16741 cctgagctta gttgcttttg gaagtccaat tattttatac attttattag ctttgattat
    16801 cccatctgcg cgttctgcac gaacaaacta tggacataac aatgcatatt atgaaaaaaa
    16861 caattatcgt gaacaaactc gtgaacaaac aaaaaaacgc aaagaagcgg aaaaaatcga
    16921 tgatgacgaa tggagtgact tttaatgtcg tactttcaac gtattttagt taatacgtta
    16981 acattcatat cattggctgt tctttttcct gcgatggtcc atgtgagcag tatttggacg
    17041 gccgttcttg ccagcttcgt attgtctgtg ttgaatatgt ttgtcaaacc gattttgacg
    17101 atactgtccc tgccctttac attagtcaca ttgggattgt ttacgtttgt tatcaatgca
    17161 gcgattctta aaatgacctc cttttttgtg ggagcagaac attttagttt ttcttcattc
    17221 ggtgcagcgt tgttagtagc tatcttgatg tcgcttgtca atgtaattgt cagcgaacat
    17281 aacagtagaa aataaaagaa agctgtgccg aactatcggc gcagtttttt tatttagaat
    17341 caggaatgcg ttttctatta cattggtaat taaataaaaa cgtttttttt aattggtagt
    17401 acttttggct agacaatgat aaaatgatag agaacactct aatagaaatg aggtagcatg
    17461 aatgtcagaa gttgtaaaag ttagccaatt agtgaaaaaa ttatctttgg aaatcgtgac
    17521 aggcgacgag aaaagcttag accgtgtcat cgtaactggg gacatctcac gcccaggact
    17581 agagttgact ggttatttta attattattc ccataatcgt ttacagctat ttggcagtaa
    17641 agaaatcagt tttgcggaac ggatgatccc agaagagcgt ttgatcatca tgagaagatt
    17701 atgtagtgaa gatatgcccg catttattat ttcaagagga ttaccagctc cagaagaaat
    17761 gatccaggca gcaaatgaaa atgggatagc tgtactgcgt tctcccatct caacttctcg
    17821 tttattagga gaattatcca gttatttaga tggtcgcctt gcccctcgaa caagtgtcca
    17881 cggtgtttta gtggatgttt acggattagg tgtactgatt caaggtgaca gtgggatagg
    17941 aaaaagtgaa acagcgttgg agttgatcaa acgcggccat cgattgatcg cagacgatcg
    18001 agtagatgtt tatcagcaag atgaattaac ggtgatcgga gaaccgccta aaatcctgga
    18061 acatctgatt gaaatccgag gaatcgggat cattgatgta atgaatcttt ttggtgcgag
    18121 tgcggtacgt ggctctatgc aagtccagtt ggctgtttat ctggaagcat gggcaaaaga
    18181 taagaaatac gaccgtctgg gctctgaaga cacgaccgtt gaaatcgcgg aagtgggcat
    18241 tccacaaata aaaatccctg tcaaaacagg acgaaacgtt gcaatcatca ttgaagtagc
    18301 cgcgatgaat ttccgtgcac gtacaatggg ctttgacgca acgaaaacat ttgaagaaag
    18361 attaagtcgc ttgattgaag aaaactcagg caacgactag caaggagtga caatatgtta
    18421 gcacaaatca atcgagttgc ttttgagctt gtagggattc ctatctactg gtatgcattg
    18481 atcatcgtat caggtatcat tatcgctatg tggcttagca gcagagaggc tgttcgggta
    18541 ggattaaaag cagatgatgt gacagatttt atgttgtggg gattgcctat ctccattatt
    18601 ggcgctcgct tatattatgt tttatttgat cttccccagt atatagctga tccaatccaa
    18661 atattcaata tccgttcagg aggcttagct atttatggcg gactgattgc aggcgcgatc
    18721 acgctgtatt tttttactaa atatcatttt atttctttgt ggactttttt agatatcgct
    18781 gcaccaagtg tattattagc acaggcaatc ggtcgttggg gaaatttcat gaaccacgaa
    18841 gcatatggac cagacacgac gcgaagcttc ttagaaagtc tgcatttacc gcaattcatc
    18901 attgataata tgtatatcga agggatctat cgtcaaccaa catttcttta tgaatcactt
    18961 tggagtttga ttggatttat tttgcttttg tttttacgaa agaaaccaca ccttttgaaa
    19021 aaaggcgaag tccttttatg ttatctaatg tggtatagtt tcgggcgttt ctttattgaa
    19081 ggattacgta cagacagtct ttacttttta ggtgtgatcc gtatttctca attattatct
    19141 gctgtgttat ttgtcggtac attgattcta tttatttggc gcagaaaaaa aggaaattta
    19201 ccggattatg accgctcatt aggaaaaaat caacaaatta tctgattttt agagcgttaa
    19261 aaatgaaaaa aatttcagga gtggtaaaat gaattataga gtagcggttt taggaccagg
    19321 ctcttggggt actgctttgg cacaagtcct cgccgaaaac ggacatgacg ttcggatttg
    19381 gggacatcgt gcttcacaag tagaagaaat caacacccaa cacacgaatc aacgttattt
    19441 accagatcat tatctacccg attccatcca agcattcacc aatatggaag atgcagtaaa
    19501 agacgcagat gcggtgctat tcgtcattcc aacgaaagca atacgttctg ttgcaaaaga
    19561 actagtgcct aaattgaata caaagccagt gatcatccat gcaagtaaag gattagaaca
    19621 gggaacacat aaaaggatct ctgacatctt gttagaggaa atcccagcag aaaaaagaaa
    19681 agctgttgtt gttctttctg gaccaagcca tgcagaagaa gtagcaaaac atgacatcac
    19741 tacgatcaca gctgcaagta cagatgcaaa agcagcaaaa tatgtacaag agctgttcat
    19801 gaataattat tttcggatct ataccaatcc tgatgtgatc ggtgtagaga tgggggcagc
    19861 tttgaaaaat attattgcta ttggtgctgg cgctattcac ggcttagggt ttggcgataa
    19921 tgcaaaggca gcaatcatga cgcgcggatt agctgagatc agccgtttag gtgtagcaat
    19981 gggtgccaac ccgctgacct ttatcggatt aagcggaata ggcgatctag tcgttacttg
    20041 cacaagtgtt cattctagga actggcgcgc aggcaacttg ttaggacaag ggcagcccct
    20101 agaagaggtg ctagccaata tgggaatggt cgttgaaggt gtgaatacta cccaagcagc
    20161 gatggaacta gcccaaatga tggaaatcga aatgcccatc actcaaacaa tctatgaagt
    20221 gctgtatgaa caaaaagaaa tcaaaacagc cgcaaaagaa atcatgttac gtgatggaaa
    20281 aaaagaaaat gaattctcat taacaatttt ttgaaggagc gaatgtatat gaaagttaaa
    20341 aaagcagtta ttccagcagc aggattagga acaagatttt taccagcaac aaaagctatg
    20401 gcaaaggaaa tgttgccaat tgttgataaa ccaacaatcc agttcatcgt tgaagaagcg
    20461 ttgaaatcag gtatcgaaga catcttgatc gttacaggaa aagctaaacg accaatcgaa
    20521 gatcactttg attcaaatat tgaattagaa acaaacctaa gtgaaaaagg aaagaccgaa
    20581 ttattgaaat tagtagaaga aacgacagat gtcaacttgc atttcatccg tcaatctcat
    20641 ccaaagggtt taggacatgc cgttcttcaa gcaagagcct ttattggaaa tgaaccattt
    20701 gtcgttatgc taggcgatga tataatggaa gacgaagtac cattgacaca gcaattaatc
    20761 gatgattacg aacgtactca tgcttctacg atcgccgtta tgcaagttcc tcatgatgaa
    20821 acttcaaaat acggaattat caatccagga gaagtattgg aagatgggtt atacaatgtc
    20881 aaaaactttg ttgaaaaacc agatccaagc aaagcaccaa gtgatttagc tattattgga
    20941 cgttacttat tgactccaga aatttttgat gtattgtccg agcaacaacc aggagccgga
    21001 aatgaaatcc aactaactga cgcaatcgat acattgaata aaacacaacg cgtatttgcc
    21061 cgtaaattca caggtaaacg ttacgatgtc ggtgacaaat ttggttttat gaaaacaagt
    21121 attgaatatg gtctaacaca tccagaagta ggtgcacctt tacgtgagta catcctgtct
    21181 ttaggtaaaa cattagaagc agaaggaagc aagatcgatc ataatccaaa aggaataaca
    21241 gaaatcaaag aagaaaagaa aaaataaccc ttttttctaa cgagcttcag ctgctaattg
    21301 aataaaaagt agttgaagct cgtttttatt tgtttaaaaa acacgaaaat gctatcctta
    21361 tagaaaagag agacaagaag ggagtttgat catgtcagga ggagaaattg caggattgat
    21421 cgcagcaatc gcctttgcga tacttgtcgt ttttatcgtt cgtgtgttgt tgcaagtttc
    21481 taaaacttta gaaaaagtag atcagactgt agcagaagca aatacgacga tcgaaatcgt
    21541 cacaaaagac gtagatcttt tgtctagaca agtagaaggc ttactggtca aaagtaatga
    21601 actgctcttg gatatcaatg gaaaagtcgc aacgatcgat ccattgttca cagcagttgc
    21661 tgatttaagt gaaagtgtat cagaacttaa tcattctagt aaaaacatcg cgacgaaagt
    21721 cggcggaatc ggtaaaacga cagctaaagc gacggttgcc ggaaaagtcg gtgggacagc
    21781 gatgaaattt tttaaaaaca aaaaacatac tgaaacgaca ggaggaaaat aaaaatggct
    21841 aaaaaaggtg gatttttatt aggtgcagta gtcggaggca cagccgcagc aatcgccgcg
    21901 ttattgttag caccaaaatc tggtaaagaa cttcgggatg aattagctgc ccaagcggat
    21961 gatttgatgg atatggcttc agattacaca gatatggcga aagaaaaatc gactgagtta
    22021 acaggtattg caaaagaaaa agcgcaagat ctttctcaac aagcaggtga tctagcaggt
    22081 agtgtcaaac aaaaagtaag tgaatcagca gatgaagcag gaagtgaatc tgacgatatc
    22141 ttgttaaatc ttaaagaaca atcagctgac ttatctaatc gtttcaaaaa aacggcagaa
    22201 gatatgaaaa aacctgcaga tgaattagga agtattgcta aagatacttc agaagatatc
    22261 atgattgatg taaaagacgc tgctggtaaa gcaggagata ctgtcaatga aggaatgtca
    22321 gaagcaaaac cagttgcaga tgactcaaaa gaaatgatga aaatggcaaa agaagatgcg
    22381 aaagaagcta aaaaagaaat aaaagataca atgacagatt catctacaaa tcactaaaat
    22441 cacaaattta tagaataaaa aggtgggagc ggactagctc tcaccttttt tactcttatt
    22501 tttctaaaac aagtaattct tttggtgtag tcgtgaatgg cacacagcca tcgcttgtca
    22561 catatacaca gtcttcgata cgtaccccta ctttttcagg aatatagatt cctggttcaa
    22621 gagagaagca catcccttct tcgattacta agtcatttcc ttctacaagg gaagggaatt
    22681 cgtggacggt tgttccgatc ccgtgaccta aacggtgatt gaaatattcg ccgtagccat
    22741 agctgttgat cacgtttcgt gcgatttgat caagttcacc tgctgtcaca cctggacgga
    22801 cggcttcttg tgcggcaagc tgtgcttcaa gagtgatgtt gtagattttt tcttggaagt
    22861 cggttggttt ttgataagca attgtacgag tagcatcact gcaatagcca ttccaaacaa
    22921 cgccaagatc gaataacaca agttcattcg gtgaaatctt cgtatttcct ggtgtaccat
    22981 gagggcttgc agcatttgct cctgcaagga ctaatgtgtc gaaagacatt tgtgaaacac
    23041 cttgtttttt caattggtat tcaatttctg caatgatcgc ttgttcagtc acgccttctt
    23101 tcactgcttt aaagccaatc tcaaatgcaa catcggccca tttaccagct tcgatcagtt
    23161 tgtctttttc ctctagagtt ttgatcagtt gtagacgttg gatgatggaa gttagatcag
    23221 tgtaagaatc cgttcccgtc aagatatcag agatagcatc gaagcgctcg acagtcaagg
    23281 cgttcttttc aatccctaca ttgccaggct gaccatagcg agcacgaagt tcttttccga
    23341 tgatctccca agggttttcg ctgtctaaat agccacggac atcatacgcc caactactgt
    23401 tttgagcatc ttcaacttcc aaagcaggag tgaaaaggaa aggatcttgt tcaagaggga
    23461 taaaaagagc tagtacacgc tcatgaggat cactttcgta tccggaaaag taagcgatgt
    23521 ggccagggtc gctgatataa gccaattcga cttggtgttg tgccatccat tgacgtagtt
    23581 cattgatttt tacgttattc atttggcaac ctactttcta aattaatttt gtattcaata
    23641 attgtaccac tgatttatta aaagaccaac gttctaaaaa ctgaaaaaag taaaaatttt
    23701 catgaaaaca gagataaaac ggttgcaatt cttgagatta tttgatattc tattttagaa
    23761 atgtaagacg tattttcaga aaacgtataa aatatgaata ggagaacaca aatggaaaag
    23821 caaacaatca ctatctatga tgtagcgaga gaggcaaatg tttcgatggc tactgtttca
    23881 cgtgtcgtaa atggcaatcc aaacgttaaa ccagcaacaa gaaaaaaagt cttggaagtt
    23941 atcgatcgct tggattatcg tccaaacgca gttgcgcgtg gcttggcaag taagaaaaca
    24001 acaacagttg gtgtaatcat ccctgatgtc agcaatatgt tctttgcttc tttagcacgt
    24061 ggtatcgatg atgtggcaac aatgtacaaa tacaatatta tcttagcaaa ttcagatggc
    24121 aacgaccaaa aagaagtcaa tgtattgaat aacttactgg ctaagcaagt ggacggcgtg
    24181 atcttcatgg gtcaccatat cacagacgaa atccgtggag aattttcacg ttcaaaaact
    24241 cctgttgtat tagctggctc tattgatcca gatgaacaag taggtagcgt aaacattgat
    24301 tatgtatccg caacaaaaga tgcagtaaac aaattggcaa aaagcggcaa taaaaaaatc
    24361 gcttttgtca gtggtgcgtt gatcgatccc atcaatggac aaaatcgtct gaaaggctat
    24421 aaagaagcat taagcgaaaa taacctacca tatagcgaag gattgatctt tgaagcacaa
    24481 tacaacttca aagatggtct atctctagtc gatcggatcc ataatagcgg tgcaacagct
    24541 gtctatgttt cagatgatga attagccatc ggtattttgg atggtttatt agaccgtggc
    24601 gtgaaagtac cggaagaatt cgaaatcatc acaagtaaca attcactact gacagaagta
    24661 gctcgtcctc gtttaacaag tatcacacaa ccattatacg atttaggggc ggtatctatg
    24721 cgcctattga cgaaaatgat gaataaagaa gaagtcgaag aaaaaacgat tatcttgcct
    24781 tatggaatcg aagaaaaagg atctacaaaa taataaaagg acaaccattc agaggttgtg
    24841 tgcctgccgt ttaactctgc gttacaagga gcaatttcaa aaaacagcat ctcatgtttg
    24901 atgaatattg tgactcgtga ttcggagttg gcaggagaac accgtttatt tgggaaaagg
    24961 ggttatgaca aaattttgtt ataatccttt ttttgtgtct taaaaaagag agaaagtggt
    25021 tgaaattaca tttatcgtat ataaaacaat ccgtgtatag atgctgcatt ttgttcacca
    25081 ccatcaaaaa aataagggac tgtgacaata ctattgtcac aatcccttat tactgtgtat
    25141 tctattaaat caatcatctt tctcatctga ttctttagtt gcttgattcg ctcttgcata
    25201 agctgaagcg gtgttccagt aatctgtata gttttcatcg gtaccgccaa cgccaaattt
    25261 aaaggcgctt ttcttcggtc catttttcgc ccatagcgat tcaactgttt ttcctggtgt
    25321 ttggatggat tttttattga cagtaacttt tccaggtgtt tgaccagtaa atccagatac
    25381 ttttttcttc atcacatcag atgataattc gaatttctca tcagtgccaa aaatgtcagg
    25441 ttttgcttgg taagcttgat tgatcagatt ggcaagataa ttagccgaac gtatccttgc
    25501 tttgctgtcc attccagtat tgtcatcgtg ccctgcccag ctactgatgg taatagacgg
    25561 agtagagaca attagccatg aatctcggta ttcattggta gaaccagttt ttccgaccca
    25621 atctgctttt cctagatttc cgtttaagct tgagatggca tctttgaatg gtgtagtgat
    25681 ttttgcattg atcacactac gcatcatgtc attcatgatc gatgccgttg cttccgaata
    25741 gatccggaca gagttgcttt cgtgtttata aattacattt cctgcgttat ctgtgatgga
    25801 atcgatgata tagccttctt catagacgcc gccgtttgct aaagcctgaa atccgttggt
    25861 ctgttgaagc gtcgtgacat ctactgtacc taaaggagca gattcaacgc cccaattgtc
    25921 atttgccggg tagttcatct ttgccaagta gtgctcgtaa gcatattgtt tcgatccacc
    25981 ggaattcaac acatcttgat ataagtgata agccgggata ttgttggacc attctagtga
    26041 ctcacggacg gtttggaagg tgttcgatcc ttcgttagta gcattgacga ttttctcgcc
    26101 agcattttct ccttcttgcc aagttgttgc ataatctgat acacgggatt ctgatccgat
    26161 aagccccatg tcgattgcag gtccataaac aagaactggt ttgattgatg agccagcttg
    26221 acgttctgta tcgaaggcat ggttattttg attctgactg taattccgtc cgccaacgaa
    26281 tccgtagatg cggccagttc tgttatccat caatacattt ccggtttcta cttgactgcc
    26341 agcaccatca tctaacatgt atccataatt ttcaacgcca ttttgcaatg cagcatagac
    26401 atctttatcg attgtcgaat ggattgtata tcctttattt tggatcgttt gctgtgcctt
    26461 ttgataataa gcgtcactga ccgatgaatc agacatatca gcgttgtctt tttcagccaa
    26521 ttgttgtgca atgattcttg ttgcttcatt catcacagta taatacaaga attctctgtc
    26581 agtctgttct gcgatttgtt gtggtagaaa gtcttttgta aggtcatagg cttttgcttc
    26641 ttcgtattgt tctttcgtaa tttggccttc acgatacata ctgaataaaa caaaatcttt
    26701 tctggcaagg ccggcagaaa gatcttcttt tagcgcgcct gtgttcgtgt atggactata
    26761 cgtgatcggg ctttggggta atccagcgat ataagcagct tgcggcagcg tcacatcttt
    26821 tgcatctaca ccaaagattc cttttgcagc ttcctggact ccagcaatgt tttgtccctt
    26881 attattacga ccaaatggtg agacattcaa atacgtagca atgatctcat cttttgaaaa
    26941 gtatttttct acttgtgcag aaagaatgat ctcgtttgct ttacgtttga atgttgtttc
    27001 atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa gttgatccac cagaagagcc
    27061 aataccagta gcttcagata ctaatgctcg taaaacagct ttaggaacgt aaccttggtg
    27121 tttatcgaag tattcatcct ctgtgctgat aattgcagtt ttcaataaag gtgaaatctt
    27181 atcagatgaa atagtcgtgc gcatcagatc tgtctggatt tttgatatct ctgtattatc
    27241 tgcataaaca atctttgacg tttctgtaat gtttcctagt tctgtctgca gtgctttttt
    27301 agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag ccagcgccta ttccaatacc
    27361 tagagctgcg aacatcaaaa gaagaacagt aataaaaaca gtcaatgatt gaaaaactct
    27421 aaaaataatc ttaggaacaa accatttttc tttttttctt ctattctgtt tttttcgagt
    27481 atttttgcgt ggcaaaaggg tcacctcaac taatggtttt atttcgctaa ttatagcaaa
    27541 taatagaaca aaaaaacaga caaggctgtt tttggcagga atttcttttt taaaagagtt
    27601 aaaacgtgag aaaatgatct ttattataaa agagttctta agcttttctt tagagaaaat
    27661 ttcagttact atggagaaca gggcaaagaa aaatcaatca ttttctttgt atatcatctt
    27721 accaatatat aggaattgtt agtaacaatt gcctttcaag gaggaatcca tccatgttat
    27781 taggctcaca cgtcagcatg agcggaaaga aaatgctgtt aggagcagca gaagaagcag
    27841 ccagttatgg atcttcgact tttatgatct atacaggcgc ccctcaaaac actagaagaa
    27901 aagccatcga agagatgaat attgatgccg gaaaagcgta catggcagaa aaaggtctaa
    27961 caaatatcgt cgttcatgcc ccttatatca ttaatttggg gaatacgatc aaaccagaaa
    28021 tgtttccttt tgccgtccaa tttctgagag aagagattgt tcgtgcggaa gctttaggcg
    28081 ctactcaaat tacacttcac ccaggtgctc atgtaggggc aggaacagaa gccggaattg
    28141 ctagaattat tgaaggcttg aatgaagttt tgacaaaaga acagacggca caaatcgctt
    28201 tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac atttgaggaa ttagcgcaaa
    28261 tcatcgatgg agtaaaattg aatgaaaaat tatccgttac ttttgacacc tgtcatacga
    28321 atgatgccgg ttataatatt aaagaagact tttcaggtgt aatggaagag tttgataaga
    28381 tcatcgggtt agatcggctt aaagtactcc atatcaatga ttctaaaaat ccgcaaggtt
    28441 cgcataaaga ccggcacgcc aatattggtt ttggaacaat cggattcgac acgctgaata
    28501 agatcgtcca tgatccaatg tttgacaata tttcgaaaat cttggaaact ccctatgtag
    28561 gcaaagataa aaagaaagct tacgcaccat atggaaaaga aatcgccatg ttcaaagcgc
    28621 aaacattcga tccagaagtg tttcctgagt tattagcaga agaagtgata ctctaagatt
    28681 ttctatctga cagcagcaaa aaaggaccag gcacattgcc ttgtcctttt ttgtttaaga
    28741 aacaaaggag tactttttgc agtctttttc atcatgcatc ttctcccccc tctttttaaa
    28801 tttagacaaa caaggtatac tggtagaaaa gtaaaaaaac gaaaagtttc acaagcaagt
    28861 ggcaaaacgt gattggaagg acgaaaagat gctgaacact ttaaaaccct tgatcaacca
    28921 actaaaaaag aaacatacat actttattct tggcatagcc ctattattta caattattcg
    28981 tttgtattta tatagtaaag caatctatgg aacagacatg tcggcaacct atgatgacca
    29041 gctgctgatt cgctatgctc aaaatatttt ggacggtaaa tggctagggg agtatacgac
    29101 tacaacactt tccaaaggaa tctcctacag cttattttta gtacttgcac ataaactgca
    29161 tctttcttat gcagttttgt tcggcatgtt cagtattttt gccagtatat tggttgcact
    29221 cgcgtttaaa ccgattgtga agaacaagta tcttctttta agtatctatt tttatttttt
    29281 gttttctccg attagtttta cacatgaata ttccactcga acatatcgta atgccctcgt
    29341 gattccggct gttgagatca ttatcgcttg tttattagct atttattacc gcaaatacgg
    29401 ttctagcaga aaattgctgc catggttttt agggctttct gtcgcttttc ctttcttttg
    29461 gttcattcgc gaagattcta tctggcttct tccttttact gtagtagctt tgggtatcag
    29521 tttcgtccag atcttgctag ggcaagactt ttctttcaaa gaagtaaaga agttaaaaaa
    29581 acgactcttt cttctctcga aacgggaatg gctgaaactt tcattgtttt tacttcctat
    29641 agttggcttt tccatcgtct atcaatggat cgaatataaa aatgagacaa catacggtat
    29701 tgcaaccatc aatgaccgtt ccagtggaga atttggacgt ttgatgaaac aactgatccg
    29761 aatagatgat gaaacgaact tgaataaaga aaacagtgag atatgggtct cacatgctgc
    29821 actaaataaa gcattagccg catctcctac attccatacc gtttctaccc gtattgacga
    29881 attatatacg actcacgcgt ggactcaagg tggaaaagta aaagaattac ccggagatat
    29941 catcttttgg gcattgcgag atgtggtaag cgaatctggt tattaccaaa acaatgcaaa
    30001 agaaaccaat gatttttgga aaaaagtcaa ccaagaatta gaagaagcct atcacaaggg
    30061 aacaatcaaa aagaaaaaag agttctatct gatgggatca ggagatggaa agacgatgag
    30121 tgatctgcca atcgtattcg atttccttca atcaggatac gcttatgcat ggcgatataa
    30181 agatttccat caaggaggaa attatagttt cggaacgcaa gaacaagtca aacaggctca
    30241 agaattatta aaccttccgc taatggagaa ttggatcgat agtaatgaac cgagaacacc
    30301 taatttccaa ttaacaaaat cagcaaaagt cgcgaatgtc atcataaaaa tttaccaagt
    30361 tctggctatt cccgtgctga ttgcttcaat agcagggata ttacttatga tactaggaac
    30421 cgtatttgca aaagctgatc gtgcctatta tagttcctct ttgattgtgg tcaccggatt
    30481 aatactgaca caattggttt ttcttttcgg tgtctcatgg ttttgttctt atgcgccaga
    30541 aaaaaaggac tattttttaa gtgtctacac tggtgcaggc gtgccattaa cgcaattcat
    30601 cgtagtaaca agcatgctag ttttcagtaa attaaatctg aaaaaagctt tatcttctaa
    30661 ataaaaagac ccttggtagc tagattttta acctaactat caagggtctt tgtgtggaaa
    30721 aatcagtttt tattcgattt cttctaccgt ttcctgcaaa agctgttcat attttttgtt
    30781 tcctttatat aaatcaccaa tcgtccatgc caacaggaga aggacaagaa caatgattac
    30841 ataagcgatg tgatcagcca atgctagttg agcggaagtg gcttgctcgc cgaagaaact
    30901 ttcgatctgt cctggtaatc ctcgtaagtt caagtaagtt agactaatca cagagaacca
    30961 gccaagaagt tttacgatga atgaattttt aaatcggcta cccatctccg cttggctgtt
    31021 ggtcatcatc agtaaaggaa tcatcgaaaa cggcaacgca aaagccaaga acacctggga
    31081 ttcattcatt aagttattca aagcggcatg ttcttcgatc gttccttttg tacttgtaaa
    31141 caaaacacaa atcaatacag gaatcacaga aagcaatcgt gtgaccagtc ttcttagcca
    31201 aatcgggata cgcatatgga tgaatccttc catgatgact tgaccggtca acgtaccagt
    31261 aatagtagag ttctggccag aagcaagaag tgctacagca aataaagtgg acaagatacc
    31321 agattttgct acagcaatca aaataccatt gctcatagct gatggatcag ataaagcttc
    31381 atacagacca aaaaaggaag gatcttggac agaaccggtt ttgaagacag cgacacccat
    31441 gatcaatagc agtgcattga ctagaaaagc cattgacaac tggatattag aatcccaagt
    31501 agaaaaacgt acagcttgtg ctacatcttc ttcgttttgg tgatcgatct tccgagtttg
    31561 tgaaactgct gaatgaagat ataagttatg cggcatgact gttgcaccaa taatccctaa
    31621 agctccagtc agcggagttt cgccgccgat gaccggtgag tctgagaatg tatgactgtt
    31681 agggataaag ccagcaaata aactgtgcca atctgggtta gacaagccaa cttgatagac
    31741 aaaaacgaat aaaatgacga aaattaagca gacaaccagt gcttcgatct tacgaaaccc
    31801 gacttttgtc agtagtaaga gaacgaaaac atctaataca gtaataaaga cggcaatcac
    31861 caatggaata tcaaaaagta agtagagggc gattgctgca ccaatgactt ctgcaatatc
    31921 tgttgccata atggctaatt cagtcaagat ccataaaatg acacctaatg tcttacttgt
    31981 gcgtgctcgg attgcctgtg ccaaatccat ttggctcacg attcctaatt ttgctgccat
    32041 atactgtaaa agcatagcaa tcagactaga catcaaaata actgacatca ataaatattg
    32101 gaaattttgt cctccggtga tcgaagtaga ccagttaccc gggtccatat acccaactgc
    32161 tactaacgca ccaggtccag agtaagcaag aagtgtttta aaaaaacttt tcccctttgg
    32221 tacatcaact gttccattta cttcagctaa agataaacca tttgtatgtt caataagtcg
    32281 atgcttatga aattgtttat tggattgatt attcaagaaa aaaccacctt tcctatttat
    32341 ctacgattac attctacacc tcaaaaagaa aaaattaaag aaaaagattc ggtacgccta
    32401 atttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cgctggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt ttt
//
LOCUS       DACDNO010000015        46669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDNO010000015 DACDNO010000000
VERSION     DACDNO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.015"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            100..570
                     /locus_tag="IWI22_000901"
     CDS             100..570
                     /locus_tag="IWI22_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9168308.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="IWI22_000902"
     CDS             792..1895
                     /locus_tag="IWI22_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9168309.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2116..3075
                     /locus_tag="IWI22_000903"
     CDS             2116..3075
                     /locus_tag="IWI22_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9168310.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3245..3925
                     /locus_tag="IWI22_000904"
     CDS             3245..3925
                     /locus_tag="IWI22_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9168311.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            4015..5049
                     /locus_tag="IWI22_000905"
     CDS             4015..5049
                     /locus_tag="IWI22_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9168312.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            5063..5398
                     /locus_tag="IWI22_000906"
     CDS             5063..5398
                     /locus_tag="IWI22_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168313.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            5382..7862
                     /locus_tag="IWI22_000907"
     CDS             5382..7862
                     /locus_tag="IWI22_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168314.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7918..9477
                     /locus_tag="IWI22_000908"
     CDS             7918..9477
                     /locus_tag="IWI22_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9168315.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            9474..10838
                     /locus_tag="IWI22_000909"
     CDS             9474..10838
                     /locus_tag="IWI22_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9168316.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            11009..12079
                     /locus_tag="IWI22_000910"
     CDS             11009..12079
                     /locus_tag="IWI22_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9168317.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            12094..12762
                     /locus_tag="IWI22_000911"
     CDS             12094..12762
                     /locus_tag="IWI22_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9168318.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12782..13360
                     /locus_tag="IWI22_000912"
     CDS             12782..13360
                     /locus_tag="IWI22_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9168319.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13761..14672
                     /locus_tag="IWI22_000913"
     CDS             13761..14672
                     /locus_tag="IWI22_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9168320.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(15050..15967)
                     /locus_tag="IWI22_000914"
     CDS             complement(15050..15967)
                     /locus_tag="IWI22_000914"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9168321.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            16169..16606
                     /gene="zapA"
                     /locus_tag="IWI22_000915"
     CDS             16169..16606
                     /gene="zapA"
                     /locus_tag="IWI22_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9168322.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            16664..17212
                     /locus_tag="IWI22_000916"
     CDS             16664..17212
                     /locus_tag="IWI22_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9168323.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            17327..19687
                     /locus_tag="IWI22_000917"
     CDS             17327..19687
                     /locus_tag="IWI22_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9168324.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            19774..20088
                     /gene="trxA"
                     /locus_tag="IWI22_000918"
     CDS             19774..20088
                     /gene="trxA"
                     /locus_tag="IWI22_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9168325.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            20270..22048
                     /gene="uvrC"
                     /locus_tag="IWI22_000919"
     CDS             20270..22048
                     /gene="uvrC"
                     /locus_tag="IWI22_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9168326.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            22193..23092
                     /locus_tag="IWI22_000920"
     CDS             22193..23092
                     /locus_tag="IWI22_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9168327.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            23085..24347
                     /locus_tag="IWI22_000921"
     CDS             23085..24347
                     /locus_tag="IWI22_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9168328.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            24508..26406
                     /locus_tag="IWI22_000922"
     CDS             24508..26406
                     /locus_tag="IWI22_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9168329.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(26736..27455)
                     /locus_tag="IWI22_000923"
     CDS             complement(26736..27455)
                     /locus_tag="IWI22_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9168330.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            27792..28124
                     /locus_tag="IWI22_000924"
     CDS             27792..28124
                     /locus_tag="IWI22_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168331.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            28111..30114
                     /locus_tag="IWI22_000925"
     CDS             28111..30114
                     /locus_tag="IWI22_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9168332.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            30133..30603
                     /locus_tag="IWI22_000926"
     CDS             30133..30603
                     /locus_tag="IWI22_000926"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9168333.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            30624..31208
                     /locus_tag="IWI22_000927"
     CDS             30624..31208
                     /locus_tag="IWI22_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9168334.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            31223..32209
                     /locus_tag="IWI22_000928"
     CDS             31223..32209
                     /locus_tag="IWI22_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9168335.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            32199..32510
                     /locus_tag="IWI22_000929"
     CDS             32199..32510
                     /locus_tag="IWI22_000929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9168336.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            32532..34313
                     /locus_tag="IWI22_000930"
     CDS             32532..34313
                     /locus_tag="IWI22_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9168337.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            34306..35682
                     /locus_tag="IWI22_000931"
     CDS             34306..35682
                     /locus_tag="IWI22_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9168338.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            35689..36324
                     /locus_tag="IWI22_000932"
     CDS             35689..36324
                     /locus_tag="IWI22_000932"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9168339.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            36354..37709
                     /locus_tag="IWI22_000933"
     CDS             36354..37709
                     /locus_tag="IWI22_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9168340.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(37901..39295)
                     /locus_tag="IWI22_000934"
     CDS             complement(37901..39295)
                     /locus_tag="IWI22_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9168341.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            39387..40358
                     /locus_tag="IWI22_000935"
     CDS             39387..40358
                     /locus_tag="IWI22_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9168342.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            40355..41137
                     /locus_tag="IWI22_000936"
     CDS             40355..41137
                     /locus_tag="IWI22_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9168343.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(41306..41677)
                     /locus_tag="IWI22_000937"
     CDS             complement(41306..41677)
                     /locus_tag="IWI22_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9168344.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            41811..42254
                     /locus_tag="IWI22_000938"
     CDS             41811..42254
                     /locus_tag="IWI22_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9168345.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(42297..43106)
                     /gene="yidA"
                     /locus_tag="IWI22_000939"
     CDS             complement(42297..43106)
                     /gene="yidA"
                     /locus_tag="IWI22_000939"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9168346.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(43125..44492)
                     /locus_tag="IWI22_000940"
     CDS             complement(43125..44492)
                     /locus_tag="IWI22_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9168347.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(44476..45306)
                     /locus_tag="IWI22_000941"
     CDS             complement(44476..45306)
                     /locus_tag="IWI22_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9168348.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            45469..45906
                     /locus_tag="IWI22_000942"
     CDS             45469..45906
                     /locus_tag="IWI22_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9168349.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            45982..46602
                     /locus_tag="IWI22_000943"
     CDS             45982..46602
                     /locus_tag="IWI22_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9168350.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        15349 a         8008 c         9910 g        13402 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact tcgccgattg taaaattaag
     2041 ctagacaact aaaaaaagtc atttgtgcta cactcaaaat agttccgacg aaagaattat
     2101 aaggagtgag tacaaatgac ctataaccat cttacaccaa ctgaactcgt aatgatagaa
     2161 gcttatttca atcaatcaca acctgtttca aaagtagcca atctgttaca acgttctcga
     2221 caaactattt ataaggtcta tcgttttctt aaatctggtg gaagcgcaat gagctactat
     2281 aaacaatata agaaaaacaa acgtaactgc ggaagacatc cgattgtttt acctgatgag
     2341 caacaggaat acattcaaaa aaaggttgtt caagggtggg ctcctgatgt ccttattggt
     2401 cgagcagagt ttcctattgc ttgttcggta cgcacactgt atcgaatgtt taaagataaa
     2461 acctttgaca ctcacgattt accaatgaag ggaaaaagaa aaccaaacgg tcataaagaa
     2521 aaaagaggaa aacaagggtt tcgccggtca atccgagaca gaaagacaga ctatacccag
     2581 tttgatcatg aatttggtca cctggaaggt gacactatcg tgggtggaaa acataaaagc
     2641 gcggtcatca cattagttga acggctatct aaagtcatca ttaccttgaa acccgaaggc
     2701 agacaggcta aagacattga aaataggctg aatcaatggt ttcgttgtgt cccaaaaaac
     2761 ttatttaaat ccatcacatt tgattgtgga aaagaattct caaattggaa aaagattagt
     2821 aataccaacg atatttcaat ttattttgcg gatccaggaa caccttctca acgaggattg
     2881 aatgaacatt caaatggctt attacgtaaa gatggtttgt ataaagctat ggattttaat
     2941 ggtgtatctg aaacctttat ccaatcggtg gcatcgaaac gaaatcatat tcctagaaaa
     3001 tcattacact atcggacacc aatggaagta tttttgagtt acgtcacaaa agatgatttg
     3061 tctaacttaa tttgacaaac gaaacttaat aaaaaaggaa gaatattcat gtaaacgatt
     3121 ttattgtttt tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc
     3181 atgaggattt tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt
     3241 tattatgaaa aagaacatga ttgggacatt attactttca acacttcttc taggcggctt
     3301 ggcaacacca gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga
     3361 accaacaaat accgtggagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc
     3421 agatccggaa aatccagcaa ctggccagac aggtccttta acactggatg tagttcccga
     3481 attgccattt ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc
     3541 aaaaaacgat acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca
     3601 agcacaaggg tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt
     3661 gcaaggcgct gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc
     3721 cactggacca actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat
     3781 atttacagca gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa
     3841 tatcacactg aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa
     3901 cctcgtggct ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat
     3961 actataggtg aaggattttt taccgcagtt gttgttgaaa ggatgtggtg gacaatgaat
     4021 caaagtaaac gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg
     4081 tcaatcagcc aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag
     4141 aaccaaatag ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag
     4201 acactagaag taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc
     4261 aatccggcgg taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta
     4321 gatgagacat tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat
     4381 gtatcagcac atgcagaaac aactgtacct atagaagtga aaatcccagc aaaaagcttt
     4441 aagggacgtg tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat
     4501 gctacagaag gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa
     4561 gaaagtcaag agacgataga accggatctg aaacttttat caggagattt ggacgaggtc
     4621 aacgcaaagc caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat
     4681 ttgatattta cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat
     4741 gcatttttag ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa
     4801 gcaaaagccg gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg
     4861 cgatttacac aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc
     4921 gtttttgcag tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt
     4981 ctagtgttat ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa
     5041 acaacatgat gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag
     5101 tttgctgttc ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat
     5161 tacattttat gatctaagtc caattccgca tccaagtggt tcagtcaatg gcgcatcaaa
     5221 acagcagaca cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac
     5281 gaatgaacaa aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc
     5341 gtggttgttg ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat
     5401 tttcattttt tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt
     5461 caccttcggc agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt
     5521 atgaaaatat cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc
     5581 aactaactgg ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa
     5641 atgatccttg gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa
     5701 atgtacttga tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt
     5761 tgccggaaca tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact
     5821 tgtatattgc cggtgccccc atcaaggtta ctagcggaag tttattatca gcaggaagcc
     5881 ataccttgcg ttttgggatg gggtcgtggg atacaaatac tctattgaca gatattattg
     5941 ctattattac gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag
     6001 ctaatgtcgc gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt
     6061 tgaccaaggg taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt
     6121 atgattcgaa taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag
     6181 gtagatttta tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg
     6241 caagtgcaga gataaatacg tggaatagtt acatctcacc aagtgattca acaggaaaat
     6301 ataatacatt tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct
     6361 ctaccaatag tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat
     6421 ttagtcgctt tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa
     6481 tactgactca tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca
     6541 gtgggaaaga tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt
     6601 acaagctaga cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc
     6661 tcctttcttc tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg
     6721 gttcgatcca ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag
     6781 atcaactttt ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac
     6841 tacctgcatt ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta
     6901 ctaacatgta tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc
     6961 ttcctcaaat aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc
     7021 ttagcagaca acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag
     7081 aaaatgtgac attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga
     7141 tctcacttcc tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta
     7201 tcgcagatac cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag
     7261 gagataccat tcatcttact tacaactatc aattaaatac tgtagatact tctttacaat
     7321 ccgtcagcat actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg
     7381 ctaagcttcc taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag
     7441 aattaacatt agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt
     7501 tgaagacaag ttattatgaa tcaaaaggcg atttttcttt tgatgtgcgg gatactcggc
     7561 ttccaagcac gtctccttgg caactgacag gtacattgac ttctttattt aagaatgatc
     7621 aaggtcaaga attaagcgga acaaaattat acttcaatca ttctggatca aagcaattga
     7681 ttcagcaagg tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc
     7741 tcgttgactt tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc
     7801 aacctggcgc aacgtatcaa ggcatggtca catgggaact gacagctggg ccaaccagct
     7861 aagtgattag aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg
     7921 agaaggaaac ttctgctgtt aaaatcagaa atgcttgaag gagtggactt ggaacttttc
     7981 agtttgcttg aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa
     8041 aaaggagagg tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg
     8101 aaagaagatc tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt
     8161 atccaagaag ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat
     8221 tatcattgtt acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat
     8281 ggctcatttg atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct
     8341 cgcagagtga aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg
     8401 cggctgaccg gtccagaaag tcaaattcgt tatttttatt tccaattatt ttggtttgga
     8461 cgtccctatc ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat
     8521 ctgctgcaaa aagagtttag ttttgctatt acagaagaag gacgattgaa atttgaaata
     8581 tggctttcca ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc
     8641 aatcttcctg ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg
     8701 acccgttact tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat
     8761 cttttcatga tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg
     8821 ataagcgaaa aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg
     8881 atagaagaat atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg
     8941 acagtgatgc aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc
     9001 caagaaggat taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt
     9061 gaaagattgg tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat
     9121 gcccgctttg aattatttgc tcgctatgca tctattttac ggacgctgat caagcagtta
     9181 gaattacgtc tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg
     9241 aaagaaatca tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag
     9301 aaagaaaagc agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag
     9361 tccgctaaag tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac
     9421 caatttttaa aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa
     9481 tcgtgatcat tggtgcttcc catgaaggca tcacagctgc cctaaatcta agaaaacttc
     9541 aaccgaaagc agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt
     9601 caaatggaat caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata
     9661 acatgcggac tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac
     9721 ttgatccaga caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa
     9781 cctatgataa attaatcgta gcgacaggtt cctctcctgt caccttgtat aaacaaattg
     9841 aagctgaaaa tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt
     9901 taaaagaagc aaaagaagta gtgatcttcg gtgctggtta cattggattg gaattagcag
     9961 atgctttgag aaataaagga tacattattc atctagttga ttatatgccg aatgttttat
    10021 caagatattt cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa
    10081 taaattttta tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat
    10141 ccgtacaact tctgtctcaa acaataaagg cagatatggt cattttttct gcccaaactc
    10201 gtcctaatac gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag
    10261 tcaatgaata tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag
    10321 tcagttttga caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata
    10381 tggcaagagc agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa
    10441 agataacagc agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg
    10501 aagcgccatt cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac
    10561 aatacgacga agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag
    10621 aaataattgg cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg
    10681 cggatatcgt gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc
    10741 tgtctgaata cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta
    10801 agagagcaaa tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg
    10861 tgtggggata taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg
    10921 gaagaatttt ttgagcataa tattcgccaa aaatttatca agtgataaaa ttgttgtata
    10981 aaatgattat ttcaggagga ggagttaaat gtttttagca ttaaatgaaa ttatgcattc
    11041 aaagttacgt tatgcgttag ttgcaggggt gatgtttttg atcgcctacc tggtattttt
    11101 tttgactggg ttagcttatg gcttagctca agataatcga acagcagtcg acaagtggga
    11161 ggcagattcg attgtgcttt cgaaagatgc aaattcaaat ctagggatgt ccatgattac
    11221 taagaagata gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc aaacacctgg
    11281 agtcgtgact agtaaagaca gtacagaaga aggaaaaatc aatgttagct tcttcggtat
    11341 cgataaaaat caattcatca tgcccaatct tgttgaagga aaagcttttg ataacgatga
    11401 tgaggcagta ggcgatatta gtttgaaaga agaatatggt ctagccatag gagatacagt
    11461 taagttatct ggtagtgaca agacgtttaa actaactggc tttacggatc atgccaaatt
    11521 caatgtctca ccagttctct atacaaccat caatgcttac caagaaatcc gcttcgaaaa
    11581 agaagataca agtgaaaatg cacggattaa cgcaatagtt gtcagaggta aaatcagtaa
    11641 tttacctgaa gatttagaac agatcaaaat ttctaaattc atcaatgaat tacctggata
    11701 taatgcgcaa gtattgactt ttggatttat gatcggattt ttgatcgtta ttgcggctat
    11761 tgtgatcggg attttcattt atgtactgac gatgcaaaag attaatatct ttggtgtaat
    11821 gaaagcacaa gggatcacag gaggatttat tgcgcgttct gtagtggcac aaacattcat
    11881 tttatcattt gttggtattc ttcttggtct attaggaaca gtgggaacat cccttgttct
    11941 acctgatgcg gttccattcc aaagcaattg gctgtttttt ggcgtcatta gcttgttgat
    12001 gttagtagta gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa aaatcgatcc
    12061 gttgaaggca attggataag aaggaggaag aacatgaaag caatagaatt taaagaagta
    12121 gaaaaaagtt tccaagatgg cgaccaagta gttaaagcat tgaaagaaac aaccatgagt
    12181 atcgataaag gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa aagtactttt
    12241 ttgactttgg caggcggatt gcagacgcct agcagtggct ctgtatttat caatgatcaa
    12301 gaattcagta atcaaaaaga aagcgaacgc gttaaactac gttttaaaga aattggcttt
    12361 attttgcaag cttccaatct ggttcctttt ctgacagtaa aaaagcagtt attattagta
    12421 gataagatta aacatgaaca tcgtcaaaaa gaagcaaacg aattatttga gcagcttggc
    12481 gtggataagt tgatcaacaa gtatccagaa gaactatctg gtggagaacg gcaaagggtc
    12541 gcaatcgctc gtgcattgta caatgatccg tccattattc tagcagatga accaactgct
    12601 agtttggatt cagaaaaggc acgagaagta gtcgatatct tggcaaaaga atcaaaagac
    12661 aaaaataaag caacgatcat ggttactcac gatacacggc tgattgacaa atgcgataga
    12721 atatttatga tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag caggtggaaa
    12781 aatggtccat cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag cgactgactt
    12841 gtttatgtcc aagggatatc tcggcacttc aacaagagaa atcgcacaaa aagttgggat
    12901 cacccagccc aatctctatc attatttcgg tgataaagaa aaactatata cggcagtttt
    12961 agaaaaccac ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa caagcgaaac
    13021 gggatttcaa gtaaccttga caaaaatggc gcagtttttg attgagacac atctggtcga
    13081 tttgtttctg atgcttcacg atcttgaaag caatctttca aaggaaacaa gagatcatct
    13141 tttctttcta tggaagaaaa attatcgtga accttttgaa gaaatctttt cagcaaacca
    13201 atctgtgcta agaaatggga tttcaaaaga aatcgcagca aggcattttt tcttagtgtt
    13261 agcaccttac atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg tggcgcaatt
    13321 gatcgattta tatttgaatg gtgtattagg agaagcgtaa agctgttata tagggagaaa
    13381 atagttaggt gaagaagcaa gatatgacga agatcgttat atcttgcttc tttatatagg
    13441 tagtttttca taaatacaaa tttgttattc tctcatgatt atcagggtat atttttattt
    13501 attatgtcgg tgttccagga agtgttttaa tttaataacg aagaaattaa ttgtttgtag
    13561 aatgaaggaa actatttatt gggtagatga atcaccacga atagtcaaaa ggagatttca
    13621 ggatatgcca aaaatgaaaa aaatcatttt agcagtaatg ttattttcta cggttatatc
    13681 ttttacagcc ccttttttgc ttacttaagc tgattttaag ttttggccct tagtctggat
    13741 ctacgaggtg agagtcaaga atgaaaaaaa taattggagc ttttgtattt gcaatcatta
    13801 tcattattat ctgttttcaa atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg
    13861 tcagcagtac gaacagtacg gttacaaaga caaaggaatc atttgatgat acagaacaag
    13921 agacattaat caaaatcgga aaaaatgacc gtacactata tacagatgac tcgctaacta
    13981 ccccacttgc aactataaac ggcggagagc tggcggagtt tctttctgaa acaaaaaatg
    14041 cttatcagat caagacaaat gatggctata ctggatattt agccctcgaa gatggaacca
    14101 aagtcacaaa gaatatacaa acaaaaccgg aagagctatc tgatgcagtg attgtattag
    14161 atcctgggca tggcggaaat gatactgggg cattaagcaa tgatgaacaa acagaagaaa
    14221 aagatataac attaagtaca gccataaaag taaaaaaggc gctagaggat gctggagcaa
    14281 cggtttattt gacccatact acagatgaat tagtacagct tggagatata tgcgactaca
    14341 gtgaagaaaa aaaggcagat gtctttatca gtttacacgc agactctacg gaatatgcga
    14401 atgaagcaac tggaatcacg acatattatt actatggaca agaggaaaca ttagcacaga
    14461 caattgctga ctccttcact gatctgcctc tggattcaag aggaatctcc actgggaact
    14521 atcaagttct tcgagaaaat cttcaacctt ctattttaat cgagatgggt tatatgaata
    14581 atgatagtga tttaaaggaa ttagtaacag acagttatca gcagcagatt gctgcaagtt
    14641 tgacacaagc attgaccact tattttcagt aataaataag cccagccaaa caaaaatagc
    14701 tcctcatatt tttcgtttgg ccgggcttat tatcgcagat gcaagctttt aaacaccgtt
    14761 taaggaagag gtcgtgagcc tgccgttcaa ctctgagtca caaggagcaa tttcaaaaaa
    14821 cagcttctca tgtttgatga ctattgtgac ttgtgaccga ggagttggca tgagaacacc
    14881 gtttaagaaa aaggttgtga aatcaacgtt ttgttctgag tattagagaa acaaacggaa
    14941 aaatagttcc tcatattttg ccgtttgttg agctaatacc gaggatcaga tgattgaaca
    15001 ccgtttattc ggaacaaggg actgtgacat gacttttgtc acagttccat tattttttta
    15061 gtagcttcaa agctttttct gtattggcaa aatgaagttt ggcatatttt tcaagcatag
    15121 gcatgccgcc tttctccaat agttttgccg ctacttggtc agatttcgtt ccagctcctg
    15181 acggaagggt gattccagct tcagctgatg ctttgtctaa ctcttccaaa aagctggcac
    15241 gactgatgat agaagcagca gcaacagcta aatgatattg ttctccttta gtaacgaagt
    15301 acagtttttc ttgtaattga tttttttcta aacgcgcata ttttttaaaa tttgcttccg
    15361 gcgtaaactg atcgatcaaa atcgcttctg gtttttctgg ctcgattttt tttagcagca
    15421 aatggatcgc ttgattgtgc aaagcgactt tcatccgaac ggcattgtat tttggttgga
    15481 tctcattgta tttttctgga gataagacaa gcagtttata ggggatggtt ttttttaaga
    15541 tgtcagcaag ctggatgatt tggctatctt ttaactcttt tgaatctcta acaccaagtt
    15601 tacgtaattc tgcaagctga tcacgtttca cataagcagc acatactgtt agcggaccga
    15661 aatagctgcc atttccgacc tcatcactac ctaaaacgga taattgactg atattttttg
    15721 gcaaagaaga ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc ccccatttat
    15781 cagcttctat tgctgcattc gtaccttgaa acatcacttt tccggatgta taagcggtaa
    15841 tggtcgtccc gtcttttttt gcagcaaagc tagtataagg aatctgttta tttaacaaat
    15901 aagagcgata gtatgctttc atttgactaa gttggtcgtt tgttgctttg ataatgacgt
    15961 tgctcataaa attctcctcg tttttataca taaaactata ccgcaactca cgacttttcg
    16021 ttttctaaga gattgcgaga catagcattt ttttaaagtt tatcatataa tagttaagta
    16081 tgtgaaaaat atttgtggag aacggtgaaa gaaccaactt catggtacac taaataaaac
    16141 tttcatcaaa taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct acaaagcggt
    16201 gattgccaac caaacatata ctattatcgg acgggaaacc aagcatcata tggatatcgt
    16261 aacgaagctg atcaatgaac agttagcaga attgaaacaa ttatcaccac aaatggataa
    16321 tgaacaagca gcaatcttga tggctgtcaa tgcgctttcg gatcaattga agaaacaaga
    16381 aagaatattg gagctggaag aagaaacagc agaattaaag aaaaaaatga tcaaatttac
    16441 agagcttgaa aatcgtgtga aacgcattga agcaatcgag aacgaagctc gggaagtatt
    16501 gaaagaaaat ggtcaagcag atcataccat taaaaatcat gtggaagcac aacagatttt
    16561 aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa
    16621 aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
    16681 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
    16741 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
    16801 agcaagtcat ttggaattat acattccata cccagctgtc accccaacat caaagttagc
    16861 attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag gaactgcttt
    16921 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
    16981 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
    17041 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
    17101 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc
    17161 atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata
    17221 gtgctggaaa atatttcgtt ttttgtacag agtagtttat ttaagaaggc tgcgtagcgt
    17281 atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat
    17341 ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc
    17401 acaaggaaaa gaagaactgg cagaattggt accagtgagt gagaaacaaa cgatcacaaa
    17461 ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat
    17521 acctaaaatt gaaaatatcc gtccccatat gaaacggatt gagatcggag cagacttgaa
    17581 tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt
    17641 cattgatgat ctttctgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca
    17701 gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg
    17761 cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga
    17821 acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag
    17881 tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg
    17941 cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggtcagactt tatttattga
    18001 accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa
    18061 tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat
    18121 catccacaat acttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg
    18181 taaagaagta aaagccatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca
    18241 agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa
    18301 agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa
    18361 gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga
    18421 aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga
    18481 gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt
    18541 caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg
    18601 tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc
    18661 aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata cgattaatgc
    18721 cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga ttggcgttcc
    18781 tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga
    18841 tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt
    18901 agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga
    18961 ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga
    19021 aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg cggaagaaaa
    19081 agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt
    19141 gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac
    19201 tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga
    19261 cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca
    19321 gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt
    19381 tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag
    19441 tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt
    19501 agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg
    19561 aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt
    19621 caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt
    19681 taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac
    19741 tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca
    19801 tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt
    19861 ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa
    19921 ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc
    19981 atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt
    20041 gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca
    20101 aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt
    20161 aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt
    20221 tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg
    20281 aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa
    20341 aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata
    20401 ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga
    20461 atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa
    20521 aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat
    20581 aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag acaaagcact
    20641 gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg
    20701 attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca
    20761 tttgggacaa tgcctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat
    20821 cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt cagagatcca
    20881 tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga
    20941 ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact
    21001 ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc aagtgttttt
    21061 cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc
    21121 agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc
    21181 taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac
    21241 gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgagtaagaa
    21301 tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat
    21361 cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc
    21421 ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga
    21481 tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga
    21541 tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga ttcgcgaagg
    21601 gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa
    21661 agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg taaaaaacga
    21721 taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac cgcttgagcg
    21781 aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat
    21841 cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga
    21901 agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat
    21961 cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa
    22021 cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt
    22081 cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc
    22141 ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga
    22201 agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca
    22261 aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac
    22321 ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca
    22381 tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta
    22441 tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag
    22501 aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa aagggaaaaa
    22561 gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac
    22621 gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt
    22681 aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat
    22741 tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg
    22801 aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac
    22861 gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa
    22921 aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg
    22981 gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc
    23041 gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga
    23101 ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat
    23161 tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata
    23221 gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc
    23281 aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag
    23341 agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa
    23401 atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac
    23461 aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca ccagaagaag
    23521 tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag
    23581 gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt tatgtagcaa
    23641 cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt
    23701 ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt
    23761 tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta
    23821 ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag
    23881 acggtatctc attggataac ttgattggac cattcttgtg gtattctctt ctattcatga
    23941 tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag
    24001 cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc ggatacatgc
    24061 ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata
    24121 ttccattctt atcttcatat attatgccga ttcgcttagc aaatgagaca gtcggtatgg
    24181 gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt
    24241 ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta tggagttcat
    24301 tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac aatcctaaaa
    24361 tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac ctattttatt
    24421 tattttacta tattggatat atattcattg aaaatagtgt ataacaactt tataatattg
    24481 agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact ttcagaaaaa
    24541 ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    24601 ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc ttttgatcat
    24661 attctgcgta tcggttcagg aaatcttaaa gaaaagatca aagacctttt ttctgaacaa
    24721 atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga atatgcatta
    24781 acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt gatcgatctt
    24841 ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt gccgaaagaa
    24901 tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg aaaaaaagac
    24961 tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg aagaagaaaa atttttaacg
    25021 aaacttgcac agacgatcca aaaatctgtc atcgatcatt tgcatatcgt tggagatatt
    25081 tttgaccgag gaactcaagc agccaaagtg atggatcgaa tcatggattt ttcttctgta
    25141 gatatccaat ggggaaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    25201 tgtttactga cacttttacg gattgcagca cgctataatt atttatttga attagagaaa
    25261 gaatatgggt tgaatttacg tccattgttt tcttttgctc atcaaactta tcagaagaat
    25321 ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa gagagcaaga agaattagaa
    25381 aaaatccatc aggcactttc tatccttcaa ttcaaatcag aacatcagtt attggatcgt
    25441 cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa aaatcgacta tgaagcatca
    25501 acgattgatt tagaaggtca gctctatgag ttgaaaggaa cttgttttca aacgatccag
    25561 ccagatgatc caaaaaaatt cttggaagag gaacaaaaag tgattgatag tttgatgtat
    25621 tcattccagc actctttgag aatgcaaaaa catatgacgt ttttgatcga aaaaggggga
    25681 atgtatctca cgaacaatca gcatgtgctg tttcatggct gtatacctgt agacgagaag
    25741 ggacagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggcttt
    25801 tttgaaaaac agataactga aagcgcaaaa gatctgactg caaaagaaga tttagcaacc
    25861 gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt tattcggcaa gagtaaaatg
    25921 gcgacttttg aacgttattt tctcacagag cctcatacgc acgtcgagaa aagcaatcct
    25981 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttagcaga attcgacaca
    26041 aaagaagaag ggtcagcaat cattaatggg cacacgccag taaaagtaaa aaaaggggag
    26101 tcaccgatca aagcaaatgg aaaagtattt gtcattgacg gaggtttatc caaagcttat
    26161 caaaaaacga caggaattgc cggttattca ctattatgca attcttatgg tttccaaatc
    26221 gtgacacatt atccgttttt gcctattgaa caacaatttg caaaaaaatc cgaccaaaca
    26281 aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac tcaatcgtga tacaacaaaa
    26341 ggtatggaac tgcagcagca gattacccag ctcaaaaggc tgttggacta tcgaaaggac
    26401 gattgagaag aattccagtg aacattgttc catagactgg agactgagac aaaagtcgtg
    26461 tcgcagtctc tttttccgga taaacggtgt tcttctgcca actcctcggt cacaagtcac
    26521 actattcaac aaacatgaaa agctgttttt tgaaattgct ccttgtgact cagagttgaa
    26581 cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gccagtttct cggtattaag
    26641 ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa tttttcttaa tactcgaaac
    26701 taaacagctt tttcacaacc ccctcagaaa gtctattaaa attcattcac aaagaaatcg
    26761 aataagcgct gttcagatgg atgtacacgg tgggtaagtt ccggatgcca ttgaacaccg
    26821 aggattttat gcatatcatc tttgctttga accgcttcaa cgagtccatc gttagaaaaa
    26881 gcgatggctt ttaataaagg tgaaagatct ttgactgcct gatgatggta agaattcaca
    26941 aaatatggat cggggagaag gttgcttaac aaactgtctg aaacaatctt gacttcgtga
    27001 gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta ctgaccactt tggatataat
    27061 gaaagatctt gataaagtgt accttccaaa acgacattca atagttggag tccccggcag
    27121 actgcaaaaa ttggttttct ttgtttcaat gcttccaaga tcagtgcctg ttcaaattgg
    27181 tctcgttgaa tatttgtttc cgttagcttt ggatgaggat cttccatgta aagttgtgga
    27241 ctgacatctt gaccacctgc aagaataagt ttgtcgattt gtccaatcag ttgaggagct
    27301 gaatctggag acatgactgg cagaataaga ggcaatcctt gagcatctaa aactgcatcc
    27361 acgaatcctt gcggtgtata gctcacttga tttccttgga aagtatctgt tgctcgaatc
    27421 agttgatttc cggcaatgcc gatgattggt ttcatttgta ttccccctaa agtagactta
    27481 ttgtaacaag gatttaaaaa aaattaaact atgtaagact tgcccctata cttatctgtt
    27541 cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa attcatgacg atagttgaaa
    27601 atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg gcgtaagctt
    27661 caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc tgcctatact
    27721 aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc tcttttttaa
    27781 atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa gcagaagaag
    27841 aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa gatctatgca acagaaaaag
    27901 agcaggaatt ggaaacgatc caaacaaact atcgacaaca actaaaacag ttggtggaag
    27961 aaaaagaaca atttgaaata caagaattag aaaaagaaaa aatgaaattg aaagcagaag
    28021 cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa gcatcagagt caagcgatcg
    28081 gtgcgatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga aaaagtgacc
    28141 ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg aatccaaaac
    28201 gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtgga gaagtatttt
    28261 ccagatacca cgattattga taaagaagca aaattaagcg ctttaggtaa tcggttgaaa
    28321 gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca aaagaaactc
    28381 catttgaaac gtcaagaact ttccttacaa gatttggaag ccaattattc cgaagaaaat
    28441 ttcataaata aattgaaaga agtccttgat ttaaaagaaa agtgggaaca actaactgaa
    28501 gaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca gctggatatt
    28561 gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt gagtgcagca
    28621 aattttgaaa gtttcaagga agaactgagt aagcttgatg acgtttatat ggaagaaata
    28681 gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc agttgctgtt
    28741 atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta tgaacaaaat
    28801 cctaaagaaa aactgcaaga gaccaagcag caattatcca ttgttcacga gcagcaaaag
    28861 aagataacga cagcgttagg tcagtgcagt ggctatatcc aagaatttga atgggtagaa
    28921 gaagtcacac ttgccgttgc agaacgagaa aaaataaaag aacggtttat ccatgcgact
    28981 tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt gctgcatgtg
    29041 ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt tcgaaacacc aaactcagaa
    29101 gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttgtcga accttttgaa
    29161 ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac accttggatg
    29221 acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta tggtttgctg
    29281 atgctcattg ggacaatact agctcaaaaa ctacttgttc taccacgcgg gatgaaacgt
    29341 ttcgtaaaat tctttgagat tctctcgata ccatcgatca tctggggatt gatctacagt
    29401 tcatttttcg gacagtcatt accaaaagaa ctgtttggca tccgcttgcc tttcccaatc
    29461 ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt cggtctgata
    29521 cagattttgg ttggattatt tgtggcagca aaggaaaata tcaggagaaa agattacttg
    29581 agtgctgtca gtgatggatt tgcctggcaa gggatcctaa ttgggatcgt tattgcgttg
    29641 gttggtgcca tgttgataaa aaatacagca tttgtttacc ttggcgctgg gctatcgatc
    29701 attgctgcct tatgtatctt gatcgttccg attatccaaa cgccgtctaa agtcaaaggt
    29761 gctgccaaag gtgcgtataa tctttacgga ctgaccggct atatcggtga tttagtcagt
    29821 tatacacgtt tgatggcatt gggtatttca ggcggaagta ttggagcggc attcaatatg
    29881 ctagttgctt tcatgccgcc agctgctcga ttcagtgtag ggatcctttt gatcatcgct
    29941 ttacacgcac tgaatatgtt tttgacactg ctaagtgcct atgtccatgg cgcacgattg
    30001 cagtatgtcg aattttttgg gaagttttat acaggaggcg gacgtgcctt tgatccgctg
    30061 aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa aaaacaataa ataattggag
    30121 gatttttata taatgatgga ttacttaatt agtcaaaatg gtggaatggt atttgcggta
    30181 ttagcgatgg caacagcgac gattttttca gggatcggtt cagctaaagg tgttggaatg
    30241 acaggtgagg cagcagcagc attgacaaca agtcaacccg aaaaattcgg gcaggctctg
    30301 attctgcaat tgcttccagg cacgcaaggt ttgtatggat tcgttattgc ctttttgatt
    30361 tttatcaact taggaaatga catgtcagtt gtacaaggac tgaactttct aggtgcttct
    30421 ctgccgattg cttttacagg cttgttttca ggtattgccc aaggaaaagt cgcagcggca
    30481 ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat ttttgctgcg
    30541 atggttgaaa catacgcgat tttaggtttc gttatttcct tcctattagt gttgaatgcc
    30601 taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct cagatgaacg
    30661 aagcagcaga gcaggaaaga gcggcgttag aacaagaaga gcgaatgaag atcgatcaaa
    30721 actttgaaca aaaacgtacg caagttgaaa cagaacatca aaaacaaaaa gaaaaacaaa
    30781 tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg ccagcaagta gaagtacgac
    30841 aagaaaattt gaatgcgaag caggaatttt taagacgatt gtttgcagat gcggtaactg
    30901 aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat caaaaatgca ttgtattcat
    30961 tacctttgac aggaaaagta gcattcattg ctggagaaaa atcagcagcc tatctttctc
    31021 agacgttgtt ggatgaatgg aacaatgaac ttccctttat gatggtttta agtgatgaaa
    31081 ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat aattttcttt
    31141 tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc aatcaattat
    31201 tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga ttcgcggaag
    31261 agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa attctgtcga
    31321 agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg acggatatga
    31381 atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct ggttaaagga
    31441 atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgttatactt tccataatct
    31501 gaaggttttg acaaaagctg agatgacaaa tcaaaatctg gaccatttgt atatcgacga
    31561 tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt caaatgaact
    31621 tcctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag agtctaccat
    31681 tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc gacggcttgc
    31741 cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg acttgaccaa
    31801 tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca tgacgacggt
    31861 actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag ataaagatat
    31921 ggaagattac acgaattttc tattagagac agattatgct gattttctgc gccctgtcat
    31981 ccgtgaggga aatattgatc tcgtaaagct tgaacagttg aaagatgatt atctaagttc
    32041 attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag cctttttgaa
    32101 cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa gaagccattt
    32161 cgaatcagaa acaatcagaa agcgggtgag acaagtctat gacgcataag atcggtgtag
    32221 ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt cgttatggta
    32281 caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag tatggcgtga
    32341 tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt tacaaggaac
    32401 aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg atagggctgg
    32461 aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg
    32521 agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt gtaatggctg
    32581 agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta ggagttatcg
    32641 gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa gagacttctg
    32701 gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt gaattaggac
    32761 cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc tttatggaaa
    32821 tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac catgagaaaa
    32881 aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga gatgttatcg
    32941 ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat gggataaaag
    33001 gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta tgtgtcattg
    33061 aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta agacgtagtc
    33121 ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag cgagtgattg
    33181 atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct tttggtgcag
    33241 ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg gttgtctatg
    33301 tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc cctgaattga
    33361 ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct aatacatcaa
    33421 acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt gcggaatatt
    33481 tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga tgggcagaag
    33541 cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt tatccagctt
    33601 atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt gcattaggat
    33661 cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct ggaggggata
    33721 tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg ggtcttgatt
    33781 ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc tattctcttt
    33841 attcaacaga agtagggaag tatatggatc atatccttca agctgactgg gcatccatgg
    33901 tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg
    33961 tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca aaatccattc
    34021 gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact tcaagagaga
    34081 aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc cgtgcgttgt
    34141 cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt gaaaggatcg
    34201 gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt attggtgaaa
    34261 tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat taaagaatat
    34321 cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc tggtgtcaaa
    34381 tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg
    34441 gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg tatcaattta
    34501 cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc ggaagacatg
    34561 atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga gatcctgcct
    34621 gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga ttatccagat
    34681 gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt tcgtgggcaa
    34741 aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc tcagatcgcg
    34801 cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc tgctattgga
    34861 atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg cgcaatcgac
    34921 cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat cgctactcct
    34981 cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca tgttttggtt
    35041 atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc agcacggcga
    35101 gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc tactttattc
    35161 gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc gattctaaca
    35221 atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat tacagaagga
    35281 cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat tgatgtactc
    35341 ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag agaagaccat
    35401 gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta
    35461 gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc gaagtttgct
    35521 gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc gattactgaa
    35581 acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt aaaacgaatc
    35641 aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat ggcacgattg
    35701 aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac aactgccaca
    35761 agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt catcctgttg
    35821 attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga agcaatgtct
    35881 gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt atttgttttg
    35941 ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt aaaagtacct
    36001 gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata cgggtatttg
    36061 aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct tccaaaactt
    36121 ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca
    36181 cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga aacgatctat
    36241 tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt gatcaaagta
    36301 aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa acgatgatta
    36361 aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca ttaggatttt
    36421 ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt aatagtgggc
    36481 atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat
    36541 tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg atcatgcttt
    36601 tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt gcattgataa
    36661 agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat ctagaagaag
    36721 tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtg atccaattga
    36781 ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg aaaggtatct
    36841 ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat ttgttaggag
    36901 acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct gctttgatca
    36961 tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat cagaaaaaga
    37021 agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta ttaggcggat
    37081 tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg aatatgtttc
    37141 aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga tattattcag
    37201 ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg atgtacatcg
    37261 gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt gtgttgctga
    37321 tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc cgtaccatcc
    37381 gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg ttgtgtgtag
    37441 tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc atcgaatata
    37501 ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga ttgacgccag
    37561 acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga cgagttggtg
    37621 tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca aaatacaaat
    37681 atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc tagtgattta
    37741 gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt gcaaaatgtt
    37801 cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct tttttattga
    37861 aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca tattttttta
    37921 ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta gaggataggg
    37981 aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa gcgtctggaa tgataaagtc
    38041 ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca ggatcaactg
    38101 gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa agagaatcaa
    38161 aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta tttgcttcat
    38221 ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc acatttatat
    38281 taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga taagcaactc
    38341 gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg ttaggtaata
    38401 aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat gaatggatat
    38461 catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc tctcgtcttt
    38521 tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag acaccatgaa
    38581 tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat
    38641 caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta gcgtatgtat
    38701 atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg atagccaatc
    38761 cattttgaac accggaaaca atagatagag attctaagga cggaatgact ccttgagttt
    38821 ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct tggtaattaa
    38881 gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca acgaatttat
    38941 tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt acttcattgg
    39001 tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt ccaatattcg
    39061 tatacagcaa acctttctca attccatatt tataagcctt gccaacggta gtaaaattta
    39121 aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc tgtaatttat
    39181 tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga cgcttcaatt
    39241 tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata ggcattttct
    39301 ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat tttagaagtt
    39361 aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa gtagtagctt
    39421 cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg
    39481 gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa ggcaaaaata
    39541 tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca aaagtcatta
    39601 ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag attttcttgt
    39661 ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc acctggattc
    39721 gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat gcagaaacac
    39781 ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt tctaccattc
    39841 gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat acttttatcg
    39901 ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca gtagaagatg
    39961 cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca tttcttcaaa
    40021 aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact cctgatacaa
    40081 gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat tactcagctg
    40141 ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta gaggaatctt
    40201 gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat aaaggagaat
    40261 cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt tgtgcagaat
    40321 atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt acgtagttga
    40381 tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg
    40441 gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg aaacagcatt
    40501 tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc gagaaatcga
    40561 tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt acaaaatacc
    40621 tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa gaattaaaga
    40681 ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat tgtctgaata
    40741 tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt tatttttcgt
    40801 attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta tcaaaaattt
    40861 tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag
    40921 aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa
    40981 tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc
    41041 atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa
    41101 agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat
    41161 aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag acgcaaactt
    41221 tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa
    41281 tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag
    41341 ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag tcagtaatct
    41401 caagaccagc agctttatgt tgctcgtgta atttttctag atcatcagct gctatagcga
    41461 tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta
    41521 attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat
    41581 cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa
    41641 gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc cttttccttt
    41701 tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt catgagaaat
    41761 ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc atggataaac
    41821 caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca gcatatattg
    41881 tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct ttttttcttc
    41941 ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag
    42001 aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt
    42061 actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt
    42121 ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag
    42181 aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa
    42241 aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac
    42301 agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca
    42361 tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat
    42421 tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata
    42481 ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact
    42541 tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa
    42601 cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc
    42661 atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct
    42721 tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc
    42781 gaatggagat gactgccgac acggcgtgcc attacttcga tctctaggaa atcttcatgt
    42841 gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta
    42901 taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca
    42961 cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca
    43021 atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg
    43081 tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc
    43141 aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa
    43201 tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt
    43261 tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg
    43321 tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca
    43381 taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata tccgaccttt
    43441 tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca
    43501 gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca
    43561 gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct
    43621 tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg
    43681 ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc
    43741 ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata
    43801 taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga
    43861 atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca
    43921 tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact
    43981 tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct
    44041 actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg
    44101 gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca
    44161 tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg
    44221 cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca
    44281 taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa
    44341 gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat caaatctaag
    44401 attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata
    44461 ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg
    44521 ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc
    44581 ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc
    44641 tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc
    44701 aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa
    44761 tcgcttcatg acttgcccac gtttttcctg attaccgttg acacttaagt acatcataac
    44821 agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct
    44881 taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt
    44941 tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt
    45001 ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat ctgatacgat
    45061 ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatcttcca gtgtttggat
    45121 cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata
    45181 ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt
    45241 taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg
    45301 gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat actctaaaat
    45361 caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca
    45421 aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca
    45481 caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa
    45541 gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt
    45601 tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca
    45661 gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat
    45721 aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt
    45781 acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt tcgaatcgat
    45841 tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca
    45901 gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga
    45961 ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa
    46021 ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt
    46081 tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga aattcgtgat
    46141 tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc attaggggat
    46201 aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat
    46261 ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc
    46321 tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat
    46381 ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc
    46441 gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa
    46501 gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa
    46561 gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag
    46621 aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacg
//
LOCUS       DACDNO010000016        44827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDNO010000016 DACDNO010000000
VERSION     DACDNO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.016"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            212..604
                     /locus_tag="IWI22_000944"
     CDS             212..604
                     /locus_tag="IWI22_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9168351.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="IWI22_000945"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="IWI22_000945"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9168352.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="IWI22_000946"
     CDS             3165..4775
                     /locus_tag="IWI22_000946"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9168353.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="IWI22_000947"
     CDS             5067..5936
                     /locus_tag="IWI22_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9168354.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="IWI22_000948"
     CDS             6101..7372
                     /locus_tag="IWI22_000948"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9168355.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /locus_tag="IWI22_000949"
     CDS             7369..8664
                     /locus_tag="IWI22_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9168356.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="IWI22_000950"
     CDS             8763..9026
                     /locus_tag="IWI22_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9168357.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="IWI22_000951"
     CDS             9471..9920
                     /locus_tag="IWI22_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9168358.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="IWI22_000952"
     CDS             9977..11395
                     /locus_tag="IWI22_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9168359.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="IWI22_000953"
     CDS             11395..12255
                     /locus_tag="IWI22_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9168360.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="IWI22_000954"
     CDS             12429..13202
                     /locus_tag="IWI22_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9168361.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="IWI22_000955"
     CDS             13349..13714
                     /locus_tag="IWI22_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9168362.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="IWI22_000956"
     CDS             complement(13862..15070)
                     /locus_tag="IWI22_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9168363.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="IWI22_000957"
     CDS             complement(15089..15367)
                     /locus_tag="IWI22_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168364.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="IWI22_000958"
     CDS             15560..16216
                     /locus_tag="IWI22_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9168365.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="IWI22_000959"
     CDS             complement(16658..17455)
                     /locus_tag="IWI22_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9168366.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="IWI22_000960"
     CDS             complement(17452..18636)
                     /locus_tag="IWI22_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9168367.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="IWI22_000961"
     CDS             complement(18661..19908)
                     /locus_tag="IWI22_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9168368.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="IWI22_000962"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="IWI22_000962"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9168369.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="IWI22_000963"
     CDS             21888..22202
                     /locus_tag="IWI22_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9168370.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="IWI22_000964"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="IWI22_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9168371.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="IWI22_000965"
     CDS             22887..23531
                     /locus_tag="IWI22_000965"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9168372.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="IWI22_000966"
     CDS             23546..23875
                     /locus_tag="IWI22_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168373.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="IWI22_000967"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="IWI22_000967"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9168374.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="IWI22_000968"
     CDS             24863..25687
                     /locus_tag="IWI22_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9168375.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="IWI22_000969"
     CDS             25680..26027
                     /locus_tag="IWI22_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9168376.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="IWI22_000970"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="IWI22_000970"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9168377.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="IWI22_000971"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="IWI22_000971"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9168378.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWI22_000972"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="IWI22_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9168379.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWI22_000973"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="IWI22_000973"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9168380.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31684..32451
                     /locus_tag="IWI22_000974"
     CDS             31684..32451
                     /locus_tag="IWI22_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9168381.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="IWI22_000975"
     CDS             32441..34483
                     /locus_tag="IWI22_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9168382.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="IWI22_000976"
     CDS             34764..34913
                     /locus_tag="IWI22_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9168383.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="IWI22_000977"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="IWI22_000977"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9168384.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="IWI22_000978"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="IWI22_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9168385.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="IWI22_000979"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="IWI22_000979"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9168386.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="IWI22_000980"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="IWI22_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9168387.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="IWI22_000981"
     CDS             39330..40412
                     /locus_tag="IWI22_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9168388.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="IWI22_000982"
     CDS             40412..41215
                     /locus_tag="IWI22_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9168389.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="IWI22_000983"
     CDS             41285..42025
                     /locus_tag="IWI22_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9168390.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="IWI22_000984"
     CDS             42036..43040
                     /locus_tag="IWI22_000984"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9168391.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="IWI22_000985"
     CDS             complement(43085..44782)
                     /locus_tag="IWI22_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168392.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14406 a         7823 c         9337 g        13261 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgattt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt agtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcattcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagc
//
LOCUS       DACDNO010000017        42154 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDNO010000017 DACDNO010000000
VERSION     DACDNO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.017"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            98..1162
                     /locus_tag="IWI22_000986"
     CDS             98..1162
                     /locus_tag="IWI22_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9168393.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1408..1833
                     /locus_tag="IWI22_000987"
     CDS             1408..1833
                     /locus_tag="IWI22_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9168394.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1826..2275
                     /gene="nusB"
                     /locus_tag="IWI22_000988"
     CDS             1826..2275
                     /gene="nusB"
                     /locus_tag="IWI22_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9168395.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2372..3226
                     /locus_tag="IWI22_000989"
     CDS             2372..3226
                     /locus_tag="IWI22_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9168396.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3227..4564
                     /locus_tag="IWI22_000990"
     CDS             3227..4564
                     /locus_tag="IWI22_000990"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9168397.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4569..4793
                     /locus_tag="IWI22_000991"
     CDS             4569..4793
                     /locus_tag="IWI22_000991"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9168398.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4795..5685
                     /locus_tag="IWI22_000992"
     CDS             4795..5685
                     /locus_tag="IWI22_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9168399.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5694..6512
                     /locus_tag="IWI22_000993"
     CDS             5694..6512
                     /locus_tag="IWI22_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9168400.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6619..7068
                     /locus_tag="IWI22_000994"
     CDS             6619..7068
                     /locus_tag="IWI22_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9168401.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7086..8768
                     /gene="recN"
                     /locus_tag="IWI22_000995"
     CDS             7086..8768
                     /gene="recN"
                     /locus_tag="IWI22_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9168402.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8823..8939)
                     /locus_tag="IWI22_000996"
     CDS             complement(8823..8939)
                     /locus_tag="IWI22_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9168403.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9012..9968)
                     /locus_tag="IWI22_000997"
     CDS             complement(9012..9968)
                     /locus_tag="IWI22_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9168404.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10139..10507
                     /locus_tag="IWI22_000998"
     CDS             10139..10507
                     /locus_tag="IWI22_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9168405.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10677..11108
                     /gene="mraZ"
                     /locus_tag="IWI22_000999"
     CDS             10677..11108
                     /gene="mraZ"
                     /locus_tag="IWI22_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9168406.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11129..12088
                     /gene="rsmH"
                     /locus_tag="IWI22_001000"
     CDS             11129..12088
                     /gene="rsmH"
                     /locus_tag="IWI22_001000"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9168407.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12093..12494
                     /gene="ftsL"
                     /locus_tag="IWI22_001001"
     CDS             12093..12494
                     /gene="ftsL"
                     /locus_tag="IWI22_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9168408.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12495..14687
                     /locus_tag="IWI22_001002"
     CDS             12495..14687
                     /locus_tag="IWI22_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9168409.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14713..15675
                     /locus_tag="IWI22_001003"
     CDS             14713..15675
                     /locus_tag="IWI22_001003"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9168410.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15689..17050
                     /locus_tag="IWI22_001004"
     CDS             15689..17050
                     /locus_tag="IWI22_001004"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9168411.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17076..18164
                     /gene="murG"
                     /locus_tag="IWI22_001005"
     CDS             17076..18164
                     /gene="murG"
                     /locus_tag="IWI22_001005"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9168412.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18179..19399
                     /locus_tag="IWI22_001006"
     CDS             18179..19399
                     /locus_tag="IWI22_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9168413.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19555..20880
                     /gene="ftsA"
                     /locus_tag="IWI22_001007"
     CDS             19555..20880
                     /gene="ftsA"
                     /locus_tag="IWI22_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9168414.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20903..22144
                     /gene="ftsZ"
                     /locus_tag="IWI22_001008"
     CDS             20903..22144
                     /gene="ftsZ"
                     /locus_tag="IWI22_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9168415.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22161..22838
                     /locus_tag="IWI22_001009"
     CDS             22161..22838
                     /locus_tag="IWI22_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9168416.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22852..23451
                     /locus_tag="IWI22_001010"
     CDS             22852..23451
                     /locus_tag="IWI22_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9168417.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23466..23753
                     /locus_tag="IWI22_001011"
     CDS             23466..23753
                     /locus_tag="IWI22_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9168418.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23802..24584
                     /locus_tag="IWI22_001012"
     CDS             23802..24584
                     /locus_tag="IWI22_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9168419.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWAENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24605..25312
                     /locus_tag="IWI22_001013"
     CDS             24605..25312
                     /locus_tag="IWI22_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9168420.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25574..28372
                     /gene="ileS"
                     /locus_tag="IWI22_001014"
     CDS             25574..28372
                     /gene="ileS"
                     /locus_tag="IWI22_001014"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9168421.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28421..29947)
                     /locus_tag="IWI22_001015"
     CDS             complement(28421..29947)
                     /locus_tag="IWI22_001015"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9168422.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29962..30609)
                     /locus_tag="IWI22_001016"
     CDS             complement(29962..30609)
                     /locus_tag="IWI22_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9168423.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30793..31122)
                     /locus_tag="IWI22_001017"
     CDS             complement(30793..31122)
                     /locus_tag="IWI22_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9168424.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31299..32027
                     /gene="sfsA"
                     /locus_tag="IWI22_001018"
     CDS             31299..32027
                     /gene="sfsA"
                     /locus_tag="IWI22_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9168425.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32043..33056
                     /locus_tag="IWI22_001019"
     CDS             32043..33056
                     /locus_tag="IWI22_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9168426.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33056..34333
                     /locus_tag="IWI22_001020"
     CDS             33056..34333
                     /locus_tag="IWI22_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9168427.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34396..37098
                     /locus_tag="IWI22_001021"
     CDS             34396..37098
                     /locus_tag="IWI22_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9168428.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37250..37567
                     /locus_tag="IWI22_001022"
     CDS             37250..37567
                     /locus_tag="IWI22_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9168429.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37597..37917
                     /locus_tag="IWI22_001023"
     CDS             37597..37917
                     /locus_tag="IWI22_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9168430.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38025..39485
                     /locus_tag="IWI22_001024"
     CDS             38025..39485
                     /locus_tag="IWI22_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9168431.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39553..39774
                     /locus_tag="IWI22_001025"
     CDS             39553..39774
                     /locus_tag="IWI22_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168432.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39805..39987
                     /locus_tag="IWI22_001026"
     CDS             39805..39987
                     /locus_tag="IWI22_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9168433.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39987..40400
                     /locus_tag="IWI22_001027"
     CDS             39987..40400
                     /locus_tag="IWI22_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9168434.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40523..41704
                     /locus_tag="IWI22_001028"
     CDS             40523..41704
                     /locus_tag="IWI22_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9168435.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        14096 a         7160 c         9076 g        11822 t
ORIGIN      
        1 aaaaagaagt tttccggaaa tttttattta actgtcgtgt acgaagaaaa gtttgctata
       61 atggaaaaaa gcgtgcaaag aaagagggag tttcattatg atgctacgtg tagaaaaatt
      121 gcgcaaaaaa atgcaagaag aaaatttgga ttcatttttg gttaccagtc cgtacaatct
      181 tcgttactta acgaacttta caggtacgac aggattagct gttattactt tggaaaaagc
      241 attttttatt actgattttc gttataccga gcaggcggca gcgcaggctc aagggtttga
      301 aattattaaa aatgtcggac ctatttttga tgaggtagca aacttagtac gcaaagaaga
      361 actgcactca ttagcttttg aggaaacaac agtctcattt ttagaatatt cggttttaga
      421 agaaatcatc gatgcggaat tagtacctgt ttcacagatg atcgaagaac tcagagaagt
      481 gaaagatgaa gaagaaatcg ctattatcga aaaagcatgt catattgcgg atatggcata
      541 tgatcacatc ctgaaaatga tccaaccggg catgactgag attgaagtcg caaatcaact
      601 agacttttat atgcgttctc ttggcgcttc aggtgtttct tttgatacga tcgttgctag
      661 cggattgcgt tcggctatgc cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga
      721 cttgatcaca ttggactttg gttgctatta cgaagggtac gtttccgata tgacacgtac
      781 attcgctatt ggtgatcctg gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc
      841 tcaattagca gttattgatg ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt
      901 tgctagagat tatatttcaa aacatggtta cggggaagca tttggacatt ctacaggaca
      961 tgggatcggt ttagagatcc acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt
     1021 tgtaccagga aatgtgatca ctgatgaacc agggatctac ttaccaggaa tcggtggagt
     1081 acgtattgag gatgacttgt tgatcacaga agatggaaat cgtgtgttga ctcgttcacc
     1141 aaaagaattg atcatcttat agtttcatca atagaagaag taagcgtaaa aatcagtgtt
     1201 cacagacttt gttatagaaa atcactcgat ttaatgatac actatagaag aacttttaag
     1261 ctgtaagaga taataaagtt gggtgttttc tccaattcat agcagtttgc agctgatcgg
     1321 cggcagtttt cttaccacgt gatcactatg ccgcatcata gagaattcgg atgtatctcg
     1381 tgcttaggca aagggaggaa taaataaatg gcagatgaaa aaaatttgat tttaaacgca
     1441 aaccaagatt taggagagat cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt
     1501 gcagcttcta aagtagaagg cgtatacgga atgcgcggaa cttttgccag caatgtcact
     1561 gaattattag gacgtgcagc tcacggaaaa ggtgtttact tagcaaacga ggaagatggc
     1621 ttgaaagtag atctttactg ttatctagaa tatggtgttt cagttcctaa agtagcaatg
     1681 gaaatgcagg aacgggtcag ccagcaagtc cttttcatga cggatatcga tttatctgaa
     1741 gtcaatatcc atgtagtagc tgttgttcct gaaaaattag aacaaccaac aatcgaagaa
     1801 ctttttgaag acgaagagga agagaatgaa taaagaatta tcaagacacg agattcggga
     1861 aatggcattg caggctttat ttccattaga cttcaatgca gacctaacaa aagaagatgc
     1921 gattttcaac gcaatcgaat tagaccaccg cgacatgatc aacgaagatg aatcagagtt
     1981 tgttcctgtt tatcttgata cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga
     2041 taaagtgatt gaaaaacatt taaaaaacaa ttggcacatc aatcggatct caaaaatgga
     2101 tttggtgatc ttgcgtatcg cgatttttga aatgacttat gtgacagatg taccagcggc
     2161 tgttgcttta aatgaagcaa tcgagttggc aaaaacattt agtgatgacc gttcacgtaa
     2221 attcgtcaac ggtgttttat ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct
     2281 agcttttttc tatgaatcat cataaacctg ttataaatcg ggaaaattct atggtagaat
     2341 gaaaacacaa aaaatatctg ggagtggtta gatggcagaa ttgattaacg gaaaagaatt
     2401 agctgaaaaa atgcaggcag agattgctga aaaaatccaa aaattgaaag ataatggtat
     2461 ccatcccgga ttagtcgttt tattggtagg ggaaaatcca gccagccaaa tctatgtaag
     2521 aaataaagaa cgtgtagcaa aagaaatcgg aatccattca ttggtagagc gttatcctga
     2581 gacgatttct gaagacgaac tacttgcaga aattgaaaaa tacaatcaag atcctagttt
     2641 tcatggtata ttggtccaat taccattgcc aaagcatata gatgaagaaa agatcctgtt
     2701 agctatccgc cctgaaaaag acgtagacgg ttttcatccg atgaatttag gcagactcct
     2761 tgcgggtaaa ccggacaaga ttccttgtac tccatacggc atcatgaaaa tgtttgaagc
     2821 ttattctatc gatccggcgg gtaaacgagc agtagttatc ggacgaagca atatcgtcgg
     2881 taaaccgatg gctcagctac tcttgatggc agatgcaact gttactgttg ctcattctaa
     2941 aaccgaaaat ctggctgaac tggcacaaga agcagatatt ttagtcgtag caataggacg
     3001 tggacatttt gtcacaaaag aattcgtaaa accaggtgct gtagtgatag atgtagggat
     3061 gaatcgtgat gctaacggta agctgatcgg tgatgtgaaa tttgatgaag tggagcctat
     3121 tgcaggctgg atcactcctg tgccaaaggg tgtagggcca atgactatca cgatgctgat
     3181 gtatcaaacc gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg cacaagaata
     3241 tttaactgtc actacattaa cgaagtattt gaaaagaaaa tttgatgccg atccttattt
     3301 agaacgcgta tatttaacag gcgaaatatc gaatttccgt ttacgtgcca atgcccatca
     3361 atatttcagc ttaaaagatg accatgcaaa aatatctgct attatgttca aaggagcatt
     3421 ccaaaaatta cgattccagc caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc
     3481 attatatgaa gcgggaggtt cttatcaaat ctatgtagag cacatggagc ctgatggaat
     3541 aggtgcttta tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt
     3601 tcaagggcct aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc
     3661 aagcggagcg gttatccgcg atatcatcac gacggtgaaa agaagatatc caattgtcca
     3721 attggttttg ttcccaacag ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa
     3781 tatccagaga gtagaagaga tgggcaattt cgatacaatg atcatcggtc gtgggggtgg
     3841 ttcgattgag gatttatggc cgtttaatga agagcgggtt gctcgggcga tttatcaagc
     3901 taatacgccg gtgatttcat ctgtaggaca tgaaacagat accacgatcg ctgatttagt
     3961 agcagatgtt cgggctgcca ctccaacagc agctgcagaa ttagctgttc ctgttttgtc
     4021 agaagagttg atgcgcattc aagaacggca agctagattg cagcaggcgt ttgtacgcca
     4081 gatccagcgc aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc
     4141 tgaaagattg tatgaaggtc agaccatcaa attagaccag ttgaaacagc gattatatca
     4201 agctacacag caaatttatc atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt
     4261 ggaacaagtg gctccgcttt atcgagttaa agcagctcgc caagaaactg cttatctaga
     4321 aaaacgctta gaagagaaaa tccaacaata tatgaaacaa cagcagcagc gttttgctca
     4381 agctgttcaa tctcttgatt tgttgagtcc attgaaaatc atgggaagag gctacagcta
     4441 tacgacgtta aatgatcaag tagtaaaaag tgtcaccgag attcatcaag gggatttgct
     4501 gacgatccat tatcaagatg gaatcgttaa aggacaagta caagagatag acaaaggaga
     4561 ataaaaatat ggaaaatcca acttttgaag agtcactgca agaattagaa aaaattgtca
     4621 tgcagttaga acaaggagac gttccattag aatcagctct agattcgttt aaacgaggaa
     4681 tggaattaag caaacattgc caagatacat tagctaaagc agaagagacc ttgacgaaaa
     4741 tgatgacaga atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga
     4801 ttaactgact tttcagctat tcatctacct gctgtagaaa aagaaatctt gtctttttta
     4861 gatgagcata caacggatag aggactctat gatgcgatga gttattcagt gaaagcaggc
     4921 gggaaaagaa tacgtccctt attactttta acggccgttg cttcctttga tgagcctatt
     4981 gatgttcccg tctatcaagt agcagctgct ttagaaatgg tgcatacgta ttccttgatc
     5041 cacgatgatt tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc taccaatcat
     5101 aaagtattcg gcgaagcatt agctatattg gccggagatg gtttgcttac aggagcattc
     5161 cagctaatca gtatggcaca cttaggtaat tctcctaagc ttctgctgct tcagcaatta
     5221 gctgtttgtg cgggatcaca aggtatggtt gctgggcaag ctgcagatat cgagggcgaa
     5281 tcgaaaaagc tttctttaga agaattagca tttattcatg aaagaaagac agggcatttg
     5341 attcgttatg ccttgctggc tggtggcata ttagcgaagc aacctgaaga aatcttattg
     5401 ctacttcaac gattagccga acatttaggt ttagcttttc agataagaga tgatctactc
     5461 gatgtgattg gtacaacgaa aacattaggg aaaacagctg gtaaagatga acgtatggaa
     5521 aaaaatactt acccgcgttt attaggccta gaaaagacac gtgaggcgtt ggagattgag
     5581 ctgttatccg ccaacaagat catagacaaa ttagaagagg acgttttttc ttttgatggc
     5641 gggctgatgc gccagatgat caagcaattt gatgtggaga aatgagggaa agaatggaaa
     5701 aagaacgcgt agatgtatta gcggtcaaac aaggattatt tgaaacaaga gaacaagcca
     5761 aacgttcagt gatggctggt ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg
     5821 gagaaaaaat tccagtgagt agtgaattaa gagtcaaagg taaaaaactt ccttatgttt
     5881 ctcgaggggg attaaaatta gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa
     5941 aaacattgtt agatattggt gcatccactg gcggatttac tgatgcagct ttgcaaaatg
     6001 gcgcaaaaat gagttatgct ttagacgttg gctacaatca gctagcttgg aaaataagac
     6061 aagatcctag agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa cccgaagact
     6121 ttaccttagg aattccagaa gttgctgtta tcgatgtttc ttttatttca ctaaaactga
     6181 tgctgcctcc attacatgct attttaaaag aagagggaga agtgattgct ttaatcaaac
     6241 cccaattcga agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga
     6301 cccatcaaaa agttttagaa gacattttgt catttgcggc acaaggtgga tatgatgtgc
     6361 tggaattaag ttattctccg atcacggggg gagaaggaaa tatcgagttc ttggctcact
     6421 tgaggaaagt acccgaaagc ggaacaatca attcagctat taatatggca gaagtggttt
     6481 caaatgcaca cgaacagttt gaccataaat aaattgtaac tgtgaaaatc aagtgagaag
     6541 tatgcggatt cttctttatt ataccaagga aataagctat cattgattaa gaagaatatg
     6601 ctaaggagcg aagacgtaat gagaaaaaaa gatcggcatc gtttgattac tcgattgttg
     6661 agtgagcaag atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata
     6721 tctgttactc aagctacgat ttccagagat ataaaggaac tgaaactgat caaggtaccg
     6781 gcttcagatg gcggctaccg ttatagcata ccagctgaaa caaacgaaga tgtaaatact
     6841 aaattggaaa aactattaaa agacgctttc gtggcagttg accaaatgga aaaatttgtt
     6901 attttgaaaa cattgccggg aaatgcttcg gcagcagcaa atttgattga taaacgttac
     6961 aagaaagaat tattttcgat tatcaatgat gacgataatg ttttgatgat tacacgaact
     7021 gaagaccagg cagttttatt gaagaaagac ttcctgcatt acctttaagt tgaggagcga
     7081 atgtgatgct acaagaaatc agtattacta actttgcgat tattccggaa ttgcggcttt
     7141 cttttcacga aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa tccattatta
     7201 tcgatgcttt aggtctgtta gcaggaggaa gaggttcaag cgattatata cgtcaaggag
     7261 cagaaaaatg tgtgcttgaa gggttgtttg aactaccaaa acaagaagga ttctctgaac
     7321 tgatggttga attagggatc gaaacagatg aggacaatct gattgttcga cgggatatgt
     7381 ccttaactgg aaaaaatgtt tgtcgagtga atggtcatat tatcacatta gctaatctga
     7441 gaagaatcgg cagttattta gtagatatcc aaggacaaaa cgaacatcag gaactgctcc
     7501 agcctgaatc acatcttgct ttattggatc gttttggaga tgtagctttc caacagaaga
     7561 aaaagagtta tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag
     7621 tgcagcaaaa tgaaaaatcc tatgttcaga gaatagatat gctccatttc cagcaagaag
     7681 aaatagctgc tgctgatttg caagtcggag aagaagagaa attaaaagaa gaaagagaaa
     7741 aacttagcaa ttatcaaaaa attgctgatg gattagctgc aggttacgga gcactgacga
     7801 ataatgaaca aaacagtgta gatggtgttg gcttggccgt ttcagaactg caagggatcg
     7861 cccatctaga tgtggagtat gaagcaattt atgaaaatat ccaaagtgct tactatttgc
     7921 tgcaagatgc aatcggcgat atgagtcgtc agattgattt attggagtta gatgagagtc
     7981 gtttagaaga agttacgcaa cgtttagaat taatccgaca gttgaaacgt aaatacggcg
     8041 aatccattga gtcaatattg gcttactatg acgaaatcac cgaagagcta gcttcttctg
     8101 atttttcaga aggtcaattg gacaaaatga aagaggaact agagcaaaaa gagcttctac
     8161 ttcagcaaca agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa
     8221 aatccattct gcatgaattg aaaagtctgt atatggaaaa cactgagttt gaggtacgtt
     8281 tcttaaaaga agaaaataga caattgaatc atgatggatt tgaccaaatc gaattttaca
     8341 ttacgaccaa tccaggagag ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac
     8401 tgtctcggat gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta
     8461 ttatttttga tgaagtggat acaggtgtga gcggaagagt tgcacaggcc atcgcagata
     8521 aaatttctca aatatcgaaa tactctcaag ttttgtgcat cactcatctg cctcaagttg
     8581 ctgcagtcgc cgactatcaa tattatattg tgaaagcagt gattgatgga cgtacgcaaa
     8641 cttctgtttc agagttgaaa acaaaagaaa gagaagaaga aattgcacgt atgctggctg
     8701 gaagtgagat tacaaaactc acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa
     8761 ggacctgagc cacctcgcag tggttcaggt ccttttcagc agctgatagc tatattttat
     8821 ttttacatga cactcattac agcagtcaat acaacagatc caatcgttaa aatcagcatt
     8881 gtccaaatga caaattttgt tactttagca aaagtacttg tttgtttttt ttcacgcatt
     8941 gttttcaacc tcgtcattaa ttagttgatt caagtgtaca atcttttttc catttccaca
     9001 agagggcttt tttataaaaa cttccctttt tttaaataat aggtagttac gatacaaata
     9061 aataatgtta atagaactag ccatataaag gcatcatccc tttcagcaat aggtaactta
     9121 atgttcattc catagatact gccgacgatt gtcggtatag ttaaaataat cgttaaagaa
     9181 gttaaaattt tcataatatt attcagatta ttcgatacga ttgctgaaaa agtctgattc
     9241 atttgttcta ctagttttaa ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc
     9301 aatacatcac gtaaatgatt tttcaaagca tgattttctt ggaaaagttt attttgcgct
     9361 agttctttca atgcatctaa attagcagaa gtcgcttctc tgaaataaac gaggcttttt
     9421 tggatatcca tgatttgata aagttgtttg ttttcagtgg atacttgaag tttggcttcc
     9481 aaagtatcgc attgagattg aagcgtagct aaataactgt tgtaagagag tgtgacctgc
     9541 caaatgaatt gaaagaagat attgagatta ggcgaaatat cactttttgg aaaggtctgg
     9601 ctaaagactt tctttaaaaa cagtggttca tgattcgtaa ttgtgataag cttctttttg
     9661 ggcgtcaaaa taatagagag cgggtatgta tccacttgaa aataaccgct aggacttgtt
     9721 ttagcaaaag gatattgtaa caataataac gctggctttg tcaactttga ttgattgaat
     9781 tcttctgtac gggaattttc tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt
     9841 tcaaattgat gtgtcagctg atgaatctct tcttgattag gacgttctac acaataccag
     9901 atcgtttggt cagtttcctt tccggtatta cgcagtttct cattatctaa tgtaaaatat
     9961 ttgatcatga ttttcctcct gttgcctgtt tcttttatta taacggatag ggaattggaa
    10021 tacatggaat gtgtttgtaa gatatagaat ataactgcat aaatttaaat aaaatgaaac
    10081 agttgtgtaa agtatataca gtatacttga agcaacgata aaaaaggagt ttacgttcat
    10141 gggcacattc acaccactta tcttgttttg gtatatttac ccggtaatcg ttttgtttgg
    10201 ttgccaattt cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa aagcccctga
    10261 tttggcgatt ccttttttgt ggggcggtat gcatgcattg tctagaaata cagggacaat
    10321 ttctatctta ccgtttttct ttatttctgt tttgctaatg ggaatttgta ttgctgtttt
    10381 ccaagcatat tattatgaag aaattattta tccgcgttat ttcaagatga cttggcgttt
    10441 ggtttttctg ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca tactttctta
    10501 cctatgaaaa aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt
    10561 tttaaaaaaa tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa aaggtggtag
    10621 aaagtggggg attgtggtag actaatttta gttagtggag gaatgggggg ctttcaatgt
    10681 tgatgggtga attccaacat aatattgatg caaaaggacg tttaatcgtg ccctcaaaac
    10741 ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg attggatggc tgtcttttcg
    10801 gctatccgat gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg ccgttggcta
    10861 aaaaagacgc acgtactttt gtgcgattct tctattcggc tgccactgaa tgcgaacttg
    10921 ataaacaagg acgaatcaat atacccagca cgttaagaaa ttatgcagct ttgacaaaag
    10981 gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag
    11041 atttttctgc agcagcagaa gagaactttg acgaaattgc tgaaagtatg attgatttcg
    11101 gactataaga ataggaagag gctgactaat gactgaaaca tttcaacact atacagtcat
    11161 gttgaaagaa acggtagatg gattgaacat caaaccggat ggcgtttatg tagactgtac
    11221 actcggcggt gccggacata gtgaatattt attaagccaa ctaagttcta aagggcatct
    11281 gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta
    11341 tgtcgaacaa ggtatggtca catttatcaa agcgaacttc agagaattag aagaacgctt
    11401 gagtgagcat gtagataagg tagacggcat tttgtatgat ctaggggttt cctctcccca
    11461 gctagatgaa gcagaacgtg gatttagtta tcatcaagat gcaccgttgg atatgcgcat
    11521 ggatcagtca gctcctttat ccgcttatca cgtaattaat gaatattcct accatgaatt
    11581 agtaaagatt ttcttccgct atggcgagga gaaattttcc aaacaaattg ctcgtgaaat
    11641 cgaacgagta cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg aaatcattaa
    11701 acaagtcatc ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa gaatttttca
    11761 agctgtgcgg attgcagtaa atgacgaact cggtgccgaa gaggcctctt tagaacaggc
    11821 aatccggtta ttgaaagtca atggaagaat cagcgtaatc acattccatt cattggaaga
    11881 cagaattgtc aaaagtatgt ttaaagaata tagtacggta caagatctac caccgggact
    11941 tcctgtcata cctgacgaat tccaaccaga actgaaagtc attactcgta aaccaatcct
    12001 tccttcagag gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc
    12061 agaaaaaata agaatgaaag aggagtagaa tgatggcaga attaaagaaa atgcaagatt
    12121 acgaatatga tttacctgaa ttggtggatc agccggaaca acaaatgcct gattcttctt
    12181 cgaatcaaga aatcattctt ccacaatcac cggcaaaacg attaaagcag atttctgctt
    12241 tggaaaaaat tgctgtggca agcgtggcga ttgctattgt tgctttatgt gttttaacag
    12301 ttatgctgcg cacaaatatc agcggagctg aaaaagaaat cacgtcaata caaacagaaa
    12361 tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta
    12421 ctgaacggat taaacaaatc gcagaagaaa aaggattgtc tattaatgac gacaatttaa
    12481 ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg gtttatggaa aagaaaaatc
    12541 taaatccgat gaacaatcgc aaaaaagtag gtatcatttt atttgctacg agtattggat
    12601 tgttcttttt atttgccgtc cgtttctcgt atatcgttat tggaggtcat gttgctggta
    12661 cctcattagc tgaaaaaaca aaacagttgt accaaggaag tgaagtagta aaagcaaaaa
    12721 gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa
    12781 ttaaagcgat actctcaaaa acatatacct ctggggataa aaaactgtat gttgaagaaa
    12841 agaattttga taaaatagca gaaatcctgc ataagaatct ttctatagac aaaaaagatg
    12901 cactgaatat tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct
    12961 atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga agcggacatg aaaaaagaag
    13021 gtgtcgctgg tctttatttt gtcgaccatc aggctcggat gtatccaaat ggcgtctttt
    13081 cttctcattt tattggatac gcagttccag acaaagatga gaatggctta gttggtaaac
    13141 taggtttgga gtctgcttac aatgatatct tgagcggtaa agatggtaag attatttatc
    13201 aaaaagataa ttttcaaaat cctctgccag gtacagtggc agaagaagaa aaagctgtcg
    13261 acggacaaga tatctacacg actttagaca gcagactaca aagttattta gaaacactga
    13321 tggatcaagt aaatgaagaa tatcagccag aagaattgac agcagtcctg atgaaagcaa
    13381 aaaccggaga aatcttggca atgggacaac gacctacttt caatcctgaa acaatggaag
    13441 gattgactgg aaaagatgct atctggcgta atttcttggt acaagatagt tatgaaccag
    13501 gatcaaccat gaaagttttc actactgcag ctgctatcga agaaggagaa ttcaacgaga
    13561 atgaaacctt ccaatctgga aaaatccaag tagaagatgc tacgatcaat gaccatgact
    13621 ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc atggtcaagt aacgtcggga
    13681 tggtcatact ggaacagcga ttaggcggac gatggtataa ctatttacaa aaactgggat
    13741 ttggacaaag tactcattct ggattagatg atgaagtaaa cggtgcttta cctacattaa
    13801 atattgtcga ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg aatttccaaa
    13861 tgatgaaagc tttcacttct attgcgaata acggtacaat gattcaacct cgatatatca
    13921 gcaaagtggt ggatccccaa actggagaag agcgaacaac tcaaacagaa gttctagggc
    13981 aacctttttc aaaagaaaca acagaaaaag tccgtgaata tatgagagat gttgtcgaaa
    14041 gcgaaaacta cggtattgct tatggtgtat atagtgtacc tggatacaat gtatcggcaa
    14101 aaaccggaac agcccaaatc gcttctgata cagggggata tcaaacagga gatacagcct
    14161 acctgtattc gattgtggag atggttcctt ctgaagatcc agactatgtc ttgtatctaa
    14221 caatgaaaca ccctaagaca tatgatcgaa tggcattggc taaaatcgca aatccattga
    14281 tgaaacgggc aatggatttt aaagaaactg aagaagattc agatacagaa acaaaaacag
    14341 aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc
    14401 aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta
    14461 ctgccaacgg tgatcagttg atctctggag aaaaactgat cctatatact ggtggtgata
    14521 agctgatgcc tgacgtaaca ggatggtcaa aagcagatat catgaagctg gggaaaatcc
    14581 tcggtattga agttagtttt gatggagatg gttattgtgt caaacaagaa cttgctccat
    14641 atgaaaaaat cactgaagac aaacttagtt ttactttaga agaataagaa gagaaatagg
    14701 agagaaaaga gcatggaatg gacacaagca ttgattccca ttgtcagcag ttgtgcaatg
    14761 acaattgccg caatgccgct atttattggt tattttcaaa tgaaaaagca aggtcaggcg
    14821 attcgtgagg agggaccaaa gtggcacaat agcaaagccg gaactcctac aatgggcggc
    14881 ttagtgtttc tgatcggctc tattttgaca ggaatttggg ttggagcttg gcaaaaacag
    14941 ctaactccta ctcttttcat cttgcttttt gtattagcgt tatacggagt aatcggcttt
    15001 cttgatgatt ttataaaaat attcaagaaa agaaacatgg gactgaactc gaagcagaaa
    15061 ttgattggac agattctagg gggaatcatc ttttatcttg tttaccgttc tgaaggttat
    15121 ccaggtgtat tgaatttctt cggactagat ctacctttag gaatcattta tggtattttc
    15181 gctctcttct ggcttgttgg attttcaaat gcagtaaatc tgacagatgg catcgatgga
    15241 ttagttgctg gcttaggaag tatctctttt gcagcttacg gtctaattgc ttggcatcag
    15301 catcagtatg atgtattaat catttgctta agtgttttag gaggtttact gggtttcttc
    15361 gtctacaaca gaaaacctgc caagattttt atgggagatg tcggttcatt agcgttgggc
    15421 ggacttttag cggcaatttc tataatgcta aaccaagaat ggacattgct tttgattggt
    15481 ttgatctatg ttatggaaac agcaagtgtg atgcttcaag ttacttcctt taagcttaca
    15541 ggaaaacgaa tttttaaaat gtctccaatt catcatcact ttgaaatgtg cggatggtcg
    15601 gagtggaaga ttgatacagt tttttggttg atcagcattg tgacttcgtt gatcacatta
    15661 tggatcgttt ggtaggagat gacgggaaat gaaaaaaata tcaacatatg aaaataagaa
    15721 agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg gccaaattat tacatgaatt
    15781 gggcgcactt gtcaccgtta atgatggaaa accttttgac gaaaatccgg aagcacaaga
    15841 gctgctttct ttagggataa aagtaataac aggtagtcat ccaatcgaat tgttggatga
    15901 agaattttca ttgatggtca aaaatccagg gattccctac agtcatcctt tagtagcaaa
    15961 agcccaagaa atgggtattc cggtaattac ggaagttgag ctagcttacg aagtagcgga
    16021 atgcccaatt attgggatta caggaacgaa cggaaaaaca acgacaacaa cgatgacagg
    16081 actactactg aatgcaggag cagatcaagg aattgcacgt ttagcgggta atattgggta
    16141 tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga tggaattgtc
    16201 tagcttccaa ctcatgggaa tcacagattt ccgccctcat atagcggtga ttaccaatat
    16261 ctatgaagca catattgatt atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa
    16321 ccttcaaaaa aacatgacag aaaaagatta tctgatcctt aactggaatc agtcagaact
    16381 acaagaatta gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt
    16441 ggaagacggt gtgtatgctg acgattactc aatatactat aaaaaagaaa aaataatgga
    16501 aatatctgaa cttggcgtac caggtaagca taatgtagaa aatgcactag ctgctatttc
    16561 tgtagcaaaa ttgtatggta tatcaaatga agctatacga gaaacgttgc attttttcca
    16621 tggcgtacca catcgtacgc aatatgtagg agaaatccaa ggcagaaaat tttataatga
    16681 ttcaaaagca accaatattt tagcgactaa aatggctcta agtggatttg aaacgtctaa
    16741 ggtcgtgtta ttggctggcg gattagacag aggaaataca tttgatgaat taattccttc
    16801 attaaaagga atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc
    16861 tggaaaaaaa gcaggcatcg agacaatagt aacagccgat tctgtagaac aagcagttcc
    16921 tttagcatta gaaaacagta cagatggtga tgtagtttta ttatcaccag cgaatgccag
    16981 ctgggatcaa tacccaaatt ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg
    17041 attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt acaggcggag
    17101 gaacaggggg acatatttat ccagctcttg cctttgtcaa ttatgtaaag actcaagaac
    17161 cgaatgctga attcatgtat gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg
    17221 atacaggaat gcctttccat accttggaga ttcaaggatt taaacgaaaa atttccatgc
    17281 ataacatcaa gacggtccga ttgtttctta agagtatccg tcaagcaaaa aaaatcctca
    17341 gtgattttca accagatatc gttatcggta ctggaggata tgtttccgga gcggtagtgt
    17401 acgctgcagc caaaatgggt attccaacag ttatccatga acaaaacagt gtaccaggtg
    17461 ttaccaataa atttttaagc cgatatgtgg atcggatcgc tctttcattt aaagatgcgg
    17521 cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca caagaagtag
    17581 cagatactaa aaaatcagat attttgaaaa catttgattt ggatccagaa aagaaaacag
    17641 tcttgatttt tggcggtagt caaggagcac ttaaaatcaa tcaagcagta tcagtttttt
    17701 taagtgaata tattccttct tcttaccaag ttttgtatgc ttcaggagat agatattatg
    17761 aagagatcct aaataaaata aatcctgaaa tacaaaatat tagtgtccag ccttatatca
    17821 aaaatatggc agaggtcatg gcttccagtg atctgctggt ggggcgtgct ggtgcaactt
    17881 cgatcgcaga attcacagct ctggggttac cggcagtgtt ggttcccagc ccatatgtta
    17941 cgaatgatca tcagactaaa aatgcaatga gtcttgttca cgcaggagca gcaaaaatga
    18001 ttgcagataa tgaactaaca ggtgaatcac taagtcagac agtcaacgaa atcatgggtg
    18061 atgaagaatt gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc cctgatgctt
    18121 caaaacgtct ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt
    18181 gagtaagaaa aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa acaatttgac
    18241 tccttggcaa aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa accaagagcc
    18301 aaaaaaagaa gacataacat tagatacacc cctttcagat tctgaagaag aaaaaacggt
    18361 tcaagaggaa cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat
    18421 caagcaaaag aaaaacggtg gaccatataa cggttctttt ttgaaccggc tgcccaattt
    18481 aaaaagtcaa cggcacaaag tactatttcg tcggttagct ttgatcatca ctgtattggg
    18541 tataccgctg atttttttga tctattatgt ttctccttat agcaaattac aagcagttgc
    18601 agtatcaggc aataagacag tcaacagcca agaaatcatc tctgatacaa aattgtcatt
    18661 aggagaaaat gtttggggac agtattttca tcgatctact tatatcgatc gccttaaaaa
    18721 agcacagcca agaatcgaaa cagcaaatat acatttcaaa gggatgaatg aattcgattt
    18781 ggatgtaaca gaatataaag agattgcttt gatcgcaaaa aacaatcaat actacccagt
    18841 gattgaaaat ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa atctccctat
    18901 cttggaagag ttcaaagact ctgcaaaaat caaagaatta acaaagcaat acaatcagtt
    18961 atcatcagaa ttacaaaaag caatttctga aatcaagtac acgccaagag caagcaataa
    19021 aaatctgatc caattgaata tgaatgacgg caatcaagtt attgtaaata tcaataatct
    19081 tgcgaaccaa atgaaatatt attcgcaagt agccaaagat atggatgaaa aaggtgtgat
    19141 cgacatggaa gttggtatct tctcgcatcc ttacggtagc actcaagaaa cggaagaatc
    19201 gggccaatcg gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag
    19261 tgaagaggca gaaacgcaaa aagcacaaac acctggatcg tctagcacaa acggatcatc
    19321 tgattcaaat gattcaagtg aaacaagtca aatagaagat aatccaagta caactccgtc
    19381 aagtttaaat gaaaattgat aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc
    19441 agtaatttat ggtagaattg gaagcagtga atatatctat taataagtat attttaaaac
    19501 ggacgctagt tgttcgcttg tatatggaat agtaagtagg agggaatccc attcatggca
    19561 aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct
    19621 gaatatatcg acagccaaat gaatattatt ggagtaggaa acgcaaaatc agaagggatt
    19681 aaccgaggca ttatcgttga tatcgataaa acggtccaag cgatacaacg agcagtacga
    19741 caagcagaag aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac
    19801 atgcttgaag tagaaaattg ccagggaatg attgcagtta acggagattc aaaggaaatc
    19861 accgatgaag atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat acctcctgaa
    19921 cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg atgggttcga gggaatcaaa
    19981 gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt tacaggacct
    20041 aaaacgatca tccataacat acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa
    20101 atggtgatta ctcctttagc gctgactgaa tcaattcttt ctgacggaga aaaagatttt
    20161 ggtacgatcg tcattgatat gggtggcggc caaacaacaa cggctgttat gcatgataag
    20221 caattgaagt ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa agatatctct
    20281 attgtattaa atacgtcatt taacaatgca gaagcgttga aaataaacta cggggatgca
    20341 tacccagaac ggacatcacc agatgaagaa tttccagtag atgtgattgg tcagtcagaa
    20401 ccagtgaaag tcgatgaacg ctacttgtca gaaattattt cagctcgaat ggaacagatt
    20461 ttcaacaaag caaaagatgc attggaccaa atcgaagcat tagagttgcc aggtggcgtc
    20521 gtattgacag gcggagctgc tagtcttcct ggtgttgtgg acttggcaca agaaatcttc
    20581 ggcgtaaatg taaaattgta tgttccaaat cacatggggc tgagaaaccc ggtatttaca
    20641 aatgtaataa gtatcgtgga ttattcagca aacttgagtg aagtgtatca attggcaaaa
    20701 attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca
    20761 aacacatatg agagctatga agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa
    20821 gagtccggag aaaatgtaaa aaataaaatc aagggtttct ttacaaatat ttttgattaa
    20881 ttcatcctgg gaggaataag atatggaatt ctcaattgat aataatatca atgacggcgc
    20941 agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc gaatgattga
    21001 agaaaacgtt aaaggtgttg aattcattac agccaacaca gacgtgcaag cattaaaaaa
    21061 ttcaaaagct gaaacagtta tccaattagg ccccaaatat actcgtggtt taggtgctgg
    21121 ttctcagcca gaagttggac aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc
    21181 tttagacggt gcagatatga ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg
    21241 tgctgcacca atcgttgcag gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt
    21301 gacacgtcca ttcacttttg aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc
    21361 tcgtttgaaa gaaaatgtgg atacattatt aattatctca aataaccgtt tattagaagt
    21421 tgtagataaa aagacaccga tgcttgaagc tttccgtgaa gcagataatg ttttacgaca
    21481 aggtgtacaa ggtatctctg atttgatcac agcgccaggt tacgttaacc ttgactttgc
    21541 tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg atggggatcg gtgtggctag
    21601 tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac
    21661 atctattgat ggagctgaac aagtacttct taacatcaca ggcggtttgg atatgacatt
    21721 gttcgaagca caagatgctt cagatatcgt agcaaatgca gcaactggcg atgtaaatat
    21781 cattctaggt acatctatca atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc
    21841 aacaggtatc gatgaatcaa aaaaggaacg caagtctagc cgaccagcta gacaagcaca
    21901 aatgcaatca cctgctcaaa agactgtatt agatatggat caagctaaac caacttcttc
    21961 agaagaagaa aacagttttg gcgattggga tatcagacgt gaacaaaatg ttcgtcctag
    22021 agttgatgat tccaattttg acaatatcga aaaaaaagaa tttgatacat tcaaccgcga
    22081 agaaacaaaa tctcatggcg atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa
    22141 ataaaggaga ggaagagcac atgattgctg acaacttgca gaaaatcaag caagaaattc
    22201 aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt tacattagta gctgtaacaa
    22261 aatctgttga atccgctcaa gctgaagaat tggttagagc tggcgttatg aattgtgcag
    22321 aaaaccgagt agataaatta ttagaaaaga aacaagattt atttgatttt cctgaaataa
    22381 agtggcattt aattggtaat ttacagcgca gaaaggtaaa attgattgta aatgaaattg
    22441 attactttca tgcgttggac agtttaagat tagcagaaga aatccagaag cggttagaga
    22501 aagaattatc ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa catggaattt
    22561 caccagatga gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg
    22621 ttgggttgat gactatggcg cctaaagaag cttctgatga gcaaatccat aaagtgtttg
    22681 gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg ttatgcccat gcaccatgta
    22741 gtgaattaag tatggggatg agtcaagact ttcaaattgc gattgaagaa ggtgcaacat
    22801 ttatccgtgt aggaacagcg ctattcaaag acgaataaag gaggaacaaa aatgccgctt
    22861 ttaaacagag gagcaattgc taattttttc ggattagctg atgaggaaga gtacgaatac
    22921 caagattatc ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag
    22981 aatcaatcaa ctcattctgt atctgctgcg aatactcatg aaaaacaaaa aacggcgact
    23041 tcacgtcctg taacgcatga aagtattcag ccggctgaaa agaaaaaagt aattgaacta
    23101 catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa
    23161 cctaaagcaa atcttgctgg aaaaatcact gtaatggaac caagagctta ttctgaagct
    23221 atggcgattg ctaaacgaat cattgctgga gaagcaacct tagtgaattt tcatttagta
    23281 gaagaaaaac aagcgagaag aatcgttgat tttctaacag gaacagttta tgcactagat
    23341 ggagatatca aacgtgttgg agatgagatt tttctttgta caccgactgg gatggagatt
    23401 gacagttcaa ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta agaggaggaa
    23461 ctacgattta tactattgca ctattacttc atttacttca acgggcagcc tatttctata
    23521 gcattatctt gatggtatat gcattgctct cttggtttcc aggcgggtat caatcagggc
    23581 taggacgatt attagctaag atctgcgaac cgtatatcag tttgttcgat cgtctgcctt
    23641 tgaggattgg acccttggat ttcacaattt taggggcaat cctgatcttg aacttagccg
    23701 tacaaggttt agcacagatc gttatatgga taactgtcca tttagtttat tgattgaagc
    23761 aacaaaatct ttattctcag aatagatagg aggggaaaat tttggatgca aatgtatacc
    23821 agcactttcg ggcagatgaa cgtacattta tcgattcggt aggcgattgg attgaacagg
    23881 ttcaaggaca atatgcaccc tatctgaccg aatttcttga cccaagacaa tcctatattt
    23941 tagaaacact gattcgacaa agcagtgatt tgcgtttcat gttttatggc ggatatgaac
    24001 aggcagaaag aaaaagatgt ttgattttcc ccgattatta cgaacctgta gaggaagatt
    24061 ttgaaacagc attgtatgaa atccattatc ctagcaaatt tgccagccta agtcatggga
    24121 aaatccttgg aactttagtg ggaacgggga tcaaacgctc ttactttggc gatatcatct
    24181 cggacggtga tcgctggcaa gtatttattg caaatgaggt ggatcatttt gttgctgcac
    24241 aagtaacaaa gattggaaag gttgctgtac gcttggaacc aatcaaatac acagaaatga
    24301 ttatgccgaa agatagctgg acacaagaaa gaggaacagt tagttccatg cgactagaca
    24361 gtgtgatctc tgagatttat aatatttcca gacagcggtc caagcagctg atcgaagctg
    24421 gtaaagtaaa agtgaactgg gcagaaaata caagacctga ttttgtactt gaactattag
    24481 atattatttc agtcagaggt tatggcagag tccagatcca agaacttgag gggaagacga
    24541 aaaaagaaaa gtacagggta ctgttcggtg tattacgtaa ataaaataaa aaggtggttt
    24601 actcatggct ttaactccat tagatatcca aaataagaca ttcccgacaa aaatgcgggg
    24661 atataatcaa gacgaagtag atgatttctt agatttagtt gttcgtgact atgaggaatt
    24721 gactcaaaga aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa aacttgagta
    24781 cttcaatgaa ttaaaagatg ctttgaacca atcaatcatc gtagctcaag atacggctga
    24841 taaagtaaaa acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa
    24901 agcagatgag ttagttgcga acgctgaaaa acgtgcacat caattaacaa ctgatgcaga
    24961 agaaaaagca agaaaaatct tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga
    25021 aactgaagat cttaagaaaa aaacacgagt attccatcaa cgtatcagct tgatgcttga
    25081 gtctcaatta gaacaagtga aaagtccgga atgggatgaa attttacaac cattctccag
    25141 ctacgtgact gacagccatg aagtgatcaa agaagtttta gcaaaacagc ttgacaatga
    25201 aaatgatact gatgtaaact cagacacagg agtcatagaa gcacctatga cggcatttga
    25261 tacacaggct attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa
    25321 agcactttgt tattcttaaa gcgagccgat gagagtggaa gttcggtaag acactgcatg
    25381 gtgaaagatc ctctacttga gacctgcttt tccgaaatta gagtaagaag cagcggctga
    25441 cctcgttatt ggtcacagag agtttgcact agatagatgc aactaaattt gggtggtacc
    25501 acggcatgtt cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa caaataaagg
    25561 agtgtatggt tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa aattccctat
    25621 gcgtggtaat ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat
    25681 ctaccaaaaa agacaagagt tgaacgaagg aaaacctagc tttgttcttc atgatggacc
    25741 tccatatgcc aatggtaata tccatttagg acattcattg aataaaatca gtaaagacat
    25801 tatcattcgt tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag gatgggatac
    25861 acatggattg ccgattgaac aagtattggc taataaaggc gtgaaacgta aagaaatgtc
    25921 catggcagaa tacttggaaa aatgtcgtga atatgcactt tcccaagtag ataagcaacg
    25981 agcagatttc aaacgcctag gagttgcagg agattgggaa catccttatg ttacattaga
    26041 tccttcttat gaagctgcac aagtacgcgt atttggtaaa atggctgaaa aaagctatat
    26101 ttacaaagga ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt tagcggaagc
    26161 tgaaattgaa tataaagatg taaaatctcc atctattttt gtagctttca atgtcaaaga
    26221 cggcaaagat attcttgatg aagatacagc ttttgtcatt tggacgacaa caccatggac
    26281 attgccagca aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc
    26341 cgatggccgt aagtttgtcg tagcgaaaga tttgctagaa acagtccaat cagaaatcgg
    26401 ttgggaaaat gtaacagttc taaaagaatt tgctggacaa gaaatggaat atatgactgc
    26461 aagtcatcca ttctatgaac gtgaatcact tgtgatttta ggcgatcatg ttactttaga
    26521 cgctggtact ggattagtcc atactgctcc aggacatggg gaagatgact atatcgctgg
    26581 tagcaaatat aaactatctg tagtgtcacc tgtagactca aaaggattgt ttacagaaga
    26641 agcacctgga tttgaaggta tcttttatga caaagcaaat cctatgatca ctgatctatt
    26701 aaaagaaaaa ggagctttgc tgaagctaga tttctttact catagttatc ctcacgattg
    26761 gcggacgaaa aagcctgtga tctatcgggc aactccacaa tggtttgcat ctatcgataa
    26821 attccgtcag aatattttgg atgaagtaga aaaagtagag tgggttatcc catggggtaa
    26881 aacacgtttg tataatatga tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc
    26941 atggggagta ccacttccga ttttctatgc tgaaaatgga gaagcaatca tcacaccaga
    27001 aacaatcgaa catgtggcaa aattatttgc tgaacatgga tcaattatct ggtttgaaaa
    27061 agaagcgaaa gaacttcttc ctgaaggatt tacccaccca ggttctccta atggcgaatt
    27121 tacaaaagaa aaagatatca tggatgtctg gtttgattct ggttcttctc atgaagctgt
    27181 tttacgtcag cgaccagaat tatcattccc agcagatatg tatttagaag gatcagacca
    27241 atatcgtggc tggttcaact caagtatcac gactagtgtg gctatcaatg gtgtagcacc
    27301 atacaaatca gttatttctc aaggatttgc tttagatggt gaaggccgta agatgagtaa
    27361 atctttagga aacatcattg caccagaaaa agtgatcaaa caatttggtg cagatatcct
    27421 tcgcttatgg gtatctagtg tagattatga agcagatgtt cgtgtttcta tggatatctt
    27481 aagtcaagtg gcagaagtat atcgtaaaat cagaaatacg atgcgtttct tattagcgaa
    27541 tacagaagat ttcgatccta aaaaagatac tgtttcgtac aatgatttac gttctgtaga
    27601 caaatatatg accgttcgat tgaaccaagt aatcaaagaa atccgagaag aagggtacga
    27661 caaatataac ttcatgcata tttaccgtac agttatgaac ttcttgacgg ttgatctatc
    27721 gtcattctac ctggattttg ctaaagatgt agtttatatt gaagcagaag atgattatca
    27781 acgtcgttgc atgcaaactg ttttctatca aacagctgta gctttgacaa aattattgac
    27841 tcctatcatt ccgcatacag cagaagaaat ctggtcattc ctgaaagaag aagaggagta
    27901 cgttcagtta agcgagtttc ctggttatga agagtttgca aaccaagaag aattgatgga
    27961 tacctggact gctttcatgg acttccgtga taatgtctta aaagcattgg aagaagcaag
    28021 aaacagcaaa ttgattggta aatctttgga agcaaaacta actgtttatc caaaacaaca
    28081 agttcgtgaa atgttaaaag ctttggatgc agatattgca caattattga tcgtttcacc
    28141 agactacttt gaagttatgg cagcagatga acaagtgcca gataacgcta tggtatttga
    28201 tgatgttgct attacagtgg aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt
    28261 tcgtaaagat gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat
    28321 cgttgaagac ttctatcctg aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa
    28381 agggggtgtg acatgttctt tgtcacaccc ccttttgtta tcaatcgttt aattttccac
    28441 gttcacgata ccagttatct ggttgccaga tccattcgaa tccgtctttt tcaagtagtt
    28501 cgaaagctgc tttaggtccc attgatccag ctttgtagtt gggaaaatcc ggttgtgttt
    28561 ggtcccaagc ttttcgaatg acatccacga tatgccaaga ttgagcgact tcttcccaat
    28621 gagagaagtt cgttccatca ccattcaacg catctaatag taatttttcg taagcttctg
    28681 ggctattttc aaccatttct gcactatttc taaattccaa tttgattggt tccgtttcaa
    28741 atccttgccc tacttctttc ccattcaagg ataaagaaaa tccttcagtt ggctgaatat
    28801 agatcgttag aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt
    28861 tgaatacatt aacaggaact tgtttgaata cgatgttgat ccgagtccct ttttcagtca
    28921 aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca acggaagtta tcgatagtga
    28981 atttgcctgc gacgaatgtt tcagtctctg attgagcatc cactttgtct tcttcgcgat
    29041 agcccttgaa ttgtacacca tccaattcac cttgagcata ttgcccgcga acgaagtttt
    29101 ctaacacttc ttcttcgtta taaatgcgga tagctttcaa tgctttgatc ttttctgtac
    29161 ggatttcttt ttcggaaaat gctactggcg gttccattgc aagtaatgct acaacctgta
    29221 aaatatgatt ttgcaccatg tctttaagtg cgccactatg atcgtagtag cccccgcgat
    29281 cttctacacc gaggttttcg ccaaaagtaa tttggatatt gtcgatatag cgattgttcc
    29341 attgagcctc aaaaatatta ttggcaaaac ggatagctga gatgttttgg atcatttctt
    29401 ttcctaaata atgatcaatc ctgaaaatat cttgttctgg gaatacttct ctgatttgct
    29461 ggttcaattc aaaagcagac tggtaatcag aaccgaacgg tttttctatg atcaaacgat
    29521 tgaagccatt ttttgtcata atattttgtg atttcaaatg agaaacgatc gttccaaaaa
    29581 attgaggtgc catagccaaa taatagacac ggttaccgtt taattgatag tgtccatcta
    29641 atcggtcaga caattctttt aatgtctgat aatgcgttga atcatttaca tcatgggatt
    29701 gatagtaaaa atggcttgca aaggtagttg cttcttcatc tgaaggattc aatgcacgga
    29761 ttgtttcttt gacaacttca cggtagtgct catcgctcca gggacggcga gcggttccga
    29821 ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca
    29881 atttacgctg ggctaggtca cctgtaccgc cgaatatcgt aaacaataca tttttttcat
    29941 tagacatgtg gatacactcc tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa
    30001 gctgacgggg attttctttc tatcattttg gatagtgatc ggtccttcat aagctgcgat
    30061 gtcaacgatc tcatattctt cgttgatatt caggtcaata gtgactaagt aatccaacaa
    30121 ttctttttca tcaagcactc gtgcgatacg cacttttgta ccgacaggat aggactccaa
    30181 tgtttgtcgg cgttcttcat ggacaacttc ctctttttta ggaatcgatc ctccatgagg
    30241 acaatgttca ggatgattca aatagttttc taaatgatct gcaagatgtt ccgaagtcac
    30301 atgttccaat acttcagcat catcatggac ttcattccag ctatagtcta aatgttcaac
    30361 taaaaaaact tcccataagc gatgtttgcg aatcagactg ctagcttttt gcagtccgct
    30421 tgcagtcaat tgtacacctt gataagggga atgttctaca agctcttctt tcacaagttt
    30481 agaaatcatt tcactgacag atgcagctga gacatctaga ctagatacga tttgtttatt
    30541 gttgactttc gttgtatctc cacctagttc taaaatgatt ttcaaataat cttctcgatt
    30601 tggtgtcata ttcgatcatc ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa
    30661 aaaacgttaa ttgactattt ttctaaataa aaaacaaagg atttttagac gtttttacaa
    30721 caaaaaacga aagatgatag agaagactca ttctttcgtt tataaaggat atatatatac
    30781 attgggaggg atctattttt cagataacaa cttatatgaa gtgtctgcag attcgtcata
    30841 aagagtttgt tcgggataag aaaagataag ataaaaacca tactgatcac gtacgactaa
    30901 acgatattca tctatttcta ccacagatgt gccaagagac tgaccgttga tcaaaaacgt
    30961 gtaatcagat gtaatctgta attgttgttt ttgctggcta tcactaaaga accatgtacc
    31021 ttgatacttt gagagtcctt ctgacttttc cgcttgtttt ttctttttca agtaaataaa
    31081 acttgagaga acggtaatga gtgaaccaag aaaatagatc atttttttta tcaccaaatc
    31141 actcctttat gagaaaataa tcgcatagac atattctttc ttctttattt cattgtaccg
    31201 aataaaagct aaaatttctc taagactttc gactggtttt tttactgttt ttctacaaat
    31261 tatgatagga tacatagtag gaagaaaggt gacataattt gaagtatcag caagtggaaa
    31321 tagctttttt tattgaccgc ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag
    31381 aaataattat tgcgcatgtc aaaaatacag gtagaggaaa agaggtcctg ataccaggcg
    31441 ctgaagtggc tattgtattt gctcctggac ctaaacggaa aaccgcctat gatctgattg
    31501 ctgtaaaaaa gaagggtgac tggtttaata tcgatagtca attgcctaat cggctggcaa
    31561 ttgatggtat tttagacggc acgatccttt taccaggatt aactggtcca tttacggtat
    31621 acaagcgtga agtaacttat cttcactcca aatttgatat ttatttagag acggtggtcg
    31681 gacaaaaagc atttgttgaa gtcaaaggta tgacacttga aaataaagcg attggtgctt
    31741 ttcctgatgc gcccacactc agaggattga aacatatagg agaattaacg tatgccgcac
    31801 aagatggtta tgccgcttat gtcttgttta ttgctcagtt cgaacatctt catctggcaa
    31861 cgatccatga agaaatgcaa ccagcactag ctgatatggt tcgacatgcg cagcaaagcg
    31921 gagttcaaat cctcgcctat aattgtcaag tatcggcaga tgaggtaact gtcaagcagg
    31981 ccattccatt tgatgtagat atcccttttg aagatcctat acaatagaaa gaaggtaaaa
    32041 taatgatcaa cttaggtatt atcggaacaa attggattac tcatcaattt gtccaagcag
    32101 ctttaagcac aaaaaaatat gaattgacag cggtatattc tagacatttg gaaaaagcac
    32161 aggaatttgg ttctcattat gaaggggata tcgaattcgc aacagatttg aagacttttt
    32221 tcaatattac tcatatggac accatttata ttgcttctcc gaatagtctt cactttagtc
    32281 aagctaagca agcaattttg gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga
    32341 cgccaaaaga aatggatgaa atcatccatt tggcaaatga acacaatgtg tatttttttg
    32401 aagcagcaag aaatatccat gaatcaggtt ttaaaaagat tgccgattac ttaccattaa
    32461 aagacgagat aatcggtgca aatttcagct atatgaaata ttcttcgcgc tatgaccaag
    32521 tattagatgg agaagaaccg aatatcttct cgactcattt ctcaggtggt gcattgatgg
    32581 atttaggtgt ttaccttgta tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc
    32641 attatttccc aagaaagatt tctactggtg tagatggtct tggatggggg atcttacgtt
    32701 atgagaattt tgatgtatcg atccagccag gtaaaatcgg tgattcttat ttaccgtcag
    32761 agatttattt tgactcagga acgctagtga tgaatggagt caatgcaatt gaacaagcac
    32821 aatttcatga tcgcgcgcat aaagaaatca ttgatttggc ggtaacagct gcagaaaatc
    32881 caatggcaga tgaagcagag gattttgcca atgtcatggc tcatccgaaa gatccagctt
    32941 ggggattgtt gtatgaagag tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg
    33001 atttaagaaa aaatggcggc atcaaattcg atgcagataa tgaggaagat ttttaatgaa
    33061 cgaatttgta caagtatttc ccgaaaattg gcagaaaaaa tatgaggata aagggtttat
    33121 ctcaccttct cttattcaac gagcagtttt tcagccacta agtaataaac aaagtatagt
    33181 agcggtttct cctacaggtt caggtaaaac actggcttat ctttggcctc tactactaaa
    33241 cgtggaacca ggtgaagcaa gtgcgttggt catttttgct tcttctcaag aactagctat
    33301 tcaagtagca gatgttgcac gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt
    33361 ggtgggcgga gccaacgtca aacgtcagat tgagggtttg aaggaaaaac cagaaatctt
    33421 gattggtaca ccaggaagaa tattggaatt gatgaaagca aaaaaaataa aagcacatca
    33481 agtgaaaaca atggtatttg atgaagcaga ccagctattt gacgctggga acagccaaat
    33541 cattgatcaa attcttcatc aagctccaac agagtatcag ctagcttttt tttcagcgac
    33601 agcagatcgt tcccttgaaa gcattgaaaa aatcactggg aaaacgtttg agacaattga
    33661 cgtgacagca gaggacgatt ctcgaaaagg actgagacat tactttttac gtgtaccgat
    33721 acgaaagaaa gatgactact tgagacgtct cacgcatata gaagattttc atggtctgat
    33781 tttctttaat caactgactg aattaggcgt aatggaagaa aaattgctgt atagaggtgt
    33841 accggtagga agtctggcct ctgatcagaa caagctactc cgaaaagcag cgctagaaca
    33901 attcaaaaag aaaatggtca gagcactttt gaccacagat gtggcggcta gaggtttaga
    33961 tattacaggt cttccttatg tagtgaatgc agaagttccg ttaagtgaag aagcttattt
    34021 gcatcgttcg ggaagaactg gtcgaatggg taatgaagga atagtaatta cactcgtaca
    34081 agataataat ctaagagatt taaaacggct tttacgtcca tcaaatataa cacttgaaga
    34141 gattttttta tacggaggta agctgcaaac tgagcagcca accaaagaag atgcagctcc
    34201 tgcagtaaat aaaaagtatg cgaaaacttt ttctggaaag gagcagaacc cagaatataa
    34261 caaagaaaaa ataagaaaaa aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc
    34321 aaggcgcaaa taagcgcctt tttatgctat gattatgaaa acgggaacaa aattggttgc
    34381 cagaggaggg gttatgtgaa agaacgtcaa gaagagatct tgcagctatt gacagaaaac
    34441 caacaaggat tgacagcaag tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca
    34501 agccgatatt taagtgaact atacaaggcc catcatattg taaaaaccgc tggccgacca
    34561 gtcgtttact ctttgccaac ggaaaagtca aaatctgatg aagtgcatgt cgactcttct
    34621 acgcaagtga cttttgaaac actcgttgga gaaaatgatt ctttaaaagt cagcatacag
    34681 caggcaaaag ctgcgattct ttatccaccg cgggggttgc atacgattat ttttggagaa
    34741 actggtacag ggaagtcgat gtttgctgaa tgtatgtatc attttgcaat cgattcagag
    34801 atgctttctg cagatgcgcc atttgtttct ttcaattgtg cggattatgc tcaaaatccg
    34861 caactgctct ttggacacat ctttgggatc aaaaaagggg cttacacagg agcagctcaa
    34921 gatagtcccg gtctaatagc caaagcagac ggcggtatat tgtttttaga tgagatccac
    34981 cgtttaccgc cggaagggca ggaaatgctg tttactttca ttgataaagg tatttaccgt
    35041 ccgcttggag aaagtgcaca agtacatgaa gcatctgttc agatcattgg ggcgacgact
    35101 gaatcttcag aaagtttctt atctacgttc aatcgacgga ttcctatggc aatcaccttg
    35161 ccgaatcttg cttcacgttc actggatgag cgttatgaaa tcatctctct ttttatcaaa
    35221 caagaagcga atcgtttgaa tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc
    35281 atgctttatg atgcggaagg aaatatcggt caagtgaaac gagacttgaa actagtctgt
    35341 gcaaaatctt ttttacatta ccgtactcat catgagaaaa agctaatcat tcgaaaagaa
    35401 gaactttccc ttcaagtaca aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc
    35461 gaccgattca tggacagcaa aagtcagtat ttgacatttg aaccaggatt tgctgatgtt
    35521 gtctggtcac aagatccaga acgaaacatg caggtctata acgatattga ggaaaaagtc
    35581 acttctcttt ccgaaaccgg tgtggagaac attgatctag agaccctcat ttcaaaagat
    35641 gtggatgctt atttccagac atatgtcaaa gaattgacta aaagttcgat tcccaaagat
    35701 cttttgccag atgaaatctg gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg
    35761 ttagaccgta tttataatga aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca
    35821 ttggatcgtg tgagagaagg tcatatgatc gtccacccag atttgaataa cgtacggaaa
    35881 aattttaaga gggaattcca ggtagcaatc gatctttcaa cgatcattga agaagaagaa
    35941 caagttgaga ttccttttga tgaaatcggc tttattagta tgtttctttc tatcaatcta
    36001 ggagaaagtg agcctgttaa agaaaacaaa gtagatgttc tcatattgat gcacggcaga
    36061 cagactgctt caagcatgtt ggagatggca caagaactat taggaacaaa agtagggaaa
    36121 gcttttaata tgccactggt tatggaagtt caaacaatgt atgaagaagt gttacaatat
    36181 gtcaaaaact atcaagagca tttgacgaat ggcctactct tgttaacaga tatgggatca
    36241 ttaaacacct tcagtgattt gatttatgaa gagactggca ttcggacgaa agctatcacg
    36301 atgacaagta ccatgattgt gatagaagcg atacggatgg caagtgttgg aagaagctta
    36361 gaagatattt atcaaaatat ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa
    36421 gcggaaaagt ctgaaacttt acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt
    36481 gaaggagtag ctgccaaatt atatcagcgt attatgccag tgattaatca aaataaggta
    36541 gaactgatcc agatgcagtt tttagaacgt gaatcattca aaaaacacat cgatgcctta
    36601 ttggaagaat atgagatcaa agcaattgcg ggaacagttg atgttgatta tcaaaatatc
    36661 ccattttttt cagcctatga cgtttttgat gatgaaaaac taaatatttt aaagcgtata
    36721 gctgatgatg agatcccgac agctaaaatt gtcgattcac ttgctggatc attaagcgat
    36781 gtcttttcta ttgatgaact gatccgttca ttgcaaaaga cagttcagca gatccagacc
    36841 gatttgtcga ttatcgtgga acctggagta gatactggaa tcgttatcca tttagccttc
    36901 atgttagacg gattgatcaa acaagaaagc catcggcaat ttgagaaact gtctattttc
    36961 tcaaaaattt atcgtttgga aatggatatc gttcgggcaa acttgatggc actagaacga
    37021 aaatatcagg taaaaattcc agaagatgat atcgcatttc ttacacaaat gtttcttgaa
    37081 aataaaataa aaaaatgaac acactaatca tactgtttac acactaaaca tctgtgtgcg
    37141 cactataaaa aagagctaaa aagcatgaaa acgctatttc agctcttttt tttattggca
    37201 tgcttcttgc gttatataaa agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa
    37261 aacaatcatg ttagtttgtt cagcagggat gagtacgagt ttattggtca caaaaatgca
    37321 aaaagcagca gaagaaaaag ggatggaagc cgatattttt gcggtctctg cctcagatgc
    37381 agataataat ttagaatcta agaatgtcga tgtattatta ttaggtcctc aagtacgttt
    37441 catgaaagct caatttgaac aaaaacttgc gcctaaaggt attccattag atgtcatcaa
    37501 tatgcaagac tatggcatga tgaacggaga aaaagttctt gcacaagcag aaaatctaat
    37561 gaaataaatc aagtatgcag ttgggggtat aacaacgtgg aagatcaaca aaatctagaa
    37621 gccatcatgg ggttaatcat gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt
    37681 gcggctgcta aaatcggtga ttttgagtta gcggatagga aaatcgctga tgcagaagaa
    37741 tcacttatac aagcgcatca tgcacaaaca gacatgttga cacaagaagc acaaggaaat
    37801 catatgcaag tgacattatt gactgttcac agtcaagatc acttaatgac atctattgcg
    37861 tttactgatt tagcaaaaga aatcatcgaa ctttatcgtc gcgtatacag cgaataaata
    37921 caacttcttt tttgaacgga agaagaaaaa tttttgaacg gattttttat tttgttataa
    37981 ggagaactct ctttatataa aaaacttata ggaggaagta aaaaatgaac ggactatcaa
    38041 gttggatgga gaaatacatc ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag
    38101 ttgctttgcg tgatgctttt atcggcacac tgcccgcaac aatggcggga tcagtcgctg
    38161 taatgatcaa tgcaattatt cgtgatttac cgcaacagtt taacagtacc tatgctgaca
    38221 gtttagcagc agacaaagga atctttgccg tattgaatgt catcatcgga atcaatggtt
    38281 ttgtctggaa cggtacacta gcaatcgccg gattgatctt tgccttttct tgggggtata
    38341 atcttgcccg agcttatgga gtaaatgatc tagctggggg gatcgttggt ttagctacct
    38401 tgattcaagg tatttccttt ggtccagcat tatcagcagc attagatgtg aatgttcctt
    38461 caaatgtcgc agatgcaatc aatagttctg agattggtgc aacgattgca gatggcacac
    38521 tgtcagttgg attatggggt tggctaaaac tagatcattt gaatggaaac gcttatttta
    38581 cagttatgat catgggagct atttcagtca ttattttttg taaattgatg cttgccaaca
    38641 ttacgatcaa aatgcctgat tcagtaccac cagcagtatc taaagcattt gcagccattt
    38701 taccagcgac gatcgcttta tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag
    38761 ctgatgggca actgatcatt gatttggtgc aaaaatatat tgcagaacca ttcctaggat
    38821 tatctcaagg gttgggagca gtattgatcg tcacagtatt tgttcaagta ttctggttct
    38881 ttggtattca tggaccaaat gtattggcac cagtgctaga gggaatctgg ggacaagcac
    38941 aactaatcaa tattgatatt ttccaaaaag gttataacgg aaaaactggt acaccagccg
    39001 tgttagatgc aatcgaagat ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt
    39061 ttgcatggtt tggcggatca ggcggaacga tcgtcttact gatagctatc ttgattttct
    39121 ctaaacgtgc agattatcta acagtagcta aattatccat tgggcctggt atcttcaata
    39181 tcaacgaacc agtgatgttt ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat
    39241 tcttgattgc tccagtagta gcaacagcaa ttggatggac agctacttat cttggcctag
    39301 tcgctccggt atcgcagcaa gtcacatggg tagtaccgcc gttcttattg tcattcttag
    39361 caacaggagc ggattggcgg gcaccgatcg taacattagt atgtatggtc gtgactttct
    39421 tgatttgggc accatttgtc atctctgcaa acaaaatgga ccctgccgca ggcgaacaac
    39481 aataagatga acgtgaggag cgggacgctt taagtttcgt tcctcataat gaattttcga
    39541 ggaggaactg aaatgattac aattgccagt gcaatgcaac aagatgaaat caaagaattg
    39601 ttagaaggat acaatgaaga aggaatgact tttacattta aagaaaaaca aggaatcaag
    39661 ctgttctttg aaacaagtgc agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta
    39721 atcaaagcac agccttgggg aacagtcctt tattttcaat caacagctgt ttagaaaatg
    39781 gtttaaaaag ataggatggt tgagatgata aaaaacggtt tatttttatg tagtattggt
    39841 tctttgatcg tgttgtatag tttgaatcca cattcgcaaa ggtatgattt gtatagtatg
    39901 gtcacaggtc tttttctaac aggattagga atctatttct tcatgaaggg gaaaaaaaga
    39961 gaagaagcaa aggagaaaaa taactaatgg aaatcacgaa aaaattaaat attccagcat
    40021 cgttttttta cgataaagtt atggactctg tccttttcga tgtacgtaaa gcgaccggca
    40081 aatcagtaac tagaaaacag ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata
    40141 accgggctcg cataaaaatc gaagaagtca ttgaaaacca gtcttataag tttcgtacat
    40201 cgacaacgaa gaatgaattc gtcgttcatt atcaaatcaa accattggac gattcgacct
    40261 gtgaggttca ttacacagaa aaaatggagt cttatggcat gcttcaaaag ttgaatgata
    40321 tgcttttagg aacaatcttg atgtatttta aacggagacg tttcaaaaaa atgctagata
    40381 tgatcgaagc aacgtattaa atcaatgaaa catggtacta tttagttatt gaaaaatgaa
    40441 gtaagctaga aggaattttt cataggaaga tagatctttc ctgcgaatcc tcactagctt
    40501 ttttgtagga aggggcagca aaatgacaaa caaagtaaga gagctaggtg tatcttcgat
    40561 tgaggaaatg tttgatttag ccacttatgc atttaatggg gcagatacac ctgaaaggcg
    40621 agaacgtttt cgtacacttg cggagaattc ttggaattac ggattttttg atgaggagga
    40681 tcggctaaca agtcaagtca tggcgacccc ttttccagtg aatttttacg gacaagagta
    40741 cctaatggcc ggcattggct atgttgcttc ttatccagaa gcaaggggac aaggcgggat
    40801 caatcagatc atggaaaaga tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta
    40861 tctggcgcca ttctcctatc ccttctatcg gcgctatggc tatgaacaat catttgataa
    40921 aatcagttat caactaaaca gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat
    40981 aaagcgtatg gattttgaag atgcaaaagc tgcaatcaga agaatttatg aacagatgcc
    41041 ggaaaaccaa cgagctggtc tgaagcgcga agactggtgg tatacgtata aattcaagca
    41101 aaaaggcaat taccagtttg ctttgcattt tgatgataat gaggaagcaa cgggatatat
    41161 cgtttatcag cttgcaacgt cttcctttat tattgcggag tggggatttt tggatcatga
    41221 tgctttttgc agccttgttc gcttcgtaag ttctcacaat ggtgcttttg aaacatttga
    41281 atattcatgc ggctacggcg gtaatgatca aaattatcta ttagaaaatc catttgcttc
    41341 tgtaatgatc acaccttata tgatggcgag gatcgtagat atttctttgt ttttggaaaa
    41401 ttatccattt aaaaagagac aagctgcatt tgccctacaa atagaagaag atcattatgc
    41461 gaaatggaat gaaggaatcg tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa
    41521 agtagaacaa acaaaactcc caatcgttcg aggaagtatc caacatatca cgcagctatt
    41581 attagggtat cgtaaagtgg aagagttggt attccaagaa agaatacagg tgacaaaaga
    41641 atatgaagaa acgttaagct caatcttgcc acagcaacag ccaattctta atgactattt
    41701 ttgataaaaa acgggtgatg acaatatttt tgtcaccacc cttttgtttt tcatttggca
    41761 tttgaaggaa aatgttaaag taaatcctct tttttcgtaa atttgaattt tttgttttct
    41821 tttgttttag ggttttcttt tttgtggtct tggattgctt ctttcgtaat cttttcccga
    41881 ttttttttag atgaatctaa gcgttctgcg tatacttttt cttttggctt atcattcatt
    41941 ggaaaggacc tccctacatt atttcattct acctctagtt tatcatctgt taaggactag
    42001 tggaaacgtt ttgcctaact tataagctga tttttttagc agataagctt gcaagtaaaa
    42061 aacaaacaag ctacaattga tttattgaaa ttgttcctgt agctcagtag gatagagcaa
    42121 tcgcctccta agcgataggt cgctggttca attc
//
LOCUS       DACDNO010000018        41494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDNO010000018 DACDNO010000000
VERSION     DACDNO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.018"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1273..1938)
                     /locus_tag="IWI22_001029"
     CDS             complement(1273..1938)
                     /locus_tag="IWI22_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9168436.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IWI22_001030"
     CDS             2064..4100
                     /locus_tag="IWI22_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9168437.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IWI22_001031"
     CDS             4104..4742
                     /locus_tag="IWI22_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9168438.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IWI22_001032"
     CDS             4770..5198
                     /locus_tag="IWI22_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168439.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IWI22_001033"
     CDS             5655..6566
                     /locus_tag="IWI22_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9168440.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IWI22_001034"
     CDS             6783..7220
                     /locus_tag="IWI22_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168441.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IWI22_001035"
     CDS             7315..8199
                     /locus_tag="IWI22_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9168442.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IWI22_001036"
     CDS             8423..11590
                     /locus_tag="IWI22_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168443.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IWI22_001037"
     CDS             11610..12155
                     /locus_tag="IWI22_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP9168444.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IWI22_001038"
     CDS             12183..12638
                     /locus_tag="IWI22_001038"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP9168445.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IWI22_001039"
     CDS             12635..13393
                     /locus_tag="IWI22_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9168446.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            13894..15093
                     /locus_tag="IWI22_001040"
     CDS             13894..15093
                     /locus_tag="IWI22_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9168447.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            15115..15327
                     /locus_tag="IWI22_001041"
     CDS             15115..15327
                     /locus_tag="IWI22_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9168448.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            15402..15878
                     /locus_tag="IWI22_001042"
     CDS             15402..15878
                     /locus_tag="IWI22_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP9168449.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            15875..17683
                     /locus_tag="IWI22_001043"
     CDS             15875..17683
                     /locus_tag="IWI22_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9168450.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            17760..17975
                     /locus_tag="IWI22_001044"
     CDS             17760..17975
                     /locus_tag="IWI22_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP9168451.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            18067..18930
                     /locus_tag="IWI22_001045"
     CDS             18067..18930
                     /locus_tag="IWI22_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168452.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            18961..19503
                     /locus_tag="IWI22_001046"
     CDS             18961..19503
                     /locus_tag="IWI22_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP9168453.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            19517..19939
                     /locus_tag="IWI22_001047"
     CDS             19517..19939
                     /locus_tag="IWI22_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP9168454.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            19869..22268
                     /locus_tag="IWI22_001048"
     CDS             19869..22268
                     /locus_tag="IWI22_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP9168455.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            22300..24291
                     /locus_tag="IWI22_001049"
     CDS             22300..24291
                     /locus_tag="IWI22_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP9168456.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            24314..24565
                     /locus_tag="IWI22_001050"
     CDS             24314..24565
                     /locus_tag="IWI22_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP9168457.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            24555..25784
                     /locus_tag="IWI22_001051"
     CDS             24555..25784
                     /locus_tag="IWI22_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP9168458.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            25781..27862
                     /gene="topB"
                     /locus_tag="IWI22_001052"
     CDS             25781..27862
                     /gene="topB"
                     /locus_tag="IWI22_001052"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9168459.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            28011..31931
                     /locus_tag="IWI22_001053"
     CDS             28011..31931
                     /locus_tag="IWI22_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP9168460.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            31932..32876
                     /locus_tag="IWI22_001054"
     CDS             31932..32876
                     /locus_tag="IWI22_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168461.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            32880..33083
                     /locus_tag="IWI22_001055"
     CDS             32880..33083
                     /locus_tag="IWI22_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168462.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(33383..33619)
                     /locus_tag="IWI22_001056"
     CDS             complement(33383..33619)
                     /locus_tag="IWI22_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9168463.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(33802..34173)
                     /locus_tag="IWI22_001057"
     CDS             complement(33802..34173)
                     /locus_tag="IWI22_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168464.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(34170..35498)
                     /locus_tag="IWI22_001058"
     CDS             complement(34170..35498)
                     /locus_tag="IWI22_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9168465.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(35459..35788)
                     /locus_tag="IWI22_001059"
     CDS             complement(35459..35788)
                     /locus_tag="IWI22_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9168466.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(36046..36417)
                     /locus_tag="IWI22_001060"
     CDS             complement(36046..36417)
                     /locus_tag="IWI22_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9168467.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            36562..36747
                     /locus_tag="IWI22_001061"
     CDS             36562..36747
                     /locus_tag="IWI22_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9168468.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            37690..38349
                     /gene="vanR"
                     /locus_tag="IWI22_001062"
     CDS             37690..38349
                     /gene="vanR"
                     /locus_tag="IWI22_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9168469.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            38352..39695
                     /gene="vanS"
                     /locus_tag="IWI22_001063"
     CDS             38352..39695
                     /gene="vanS"
                     /locus_tag="IWI22_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9168470.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            39866..40672
                     /gene="vanY"
                     /locus_tag="IWI22_001064"
     CDS             39866..40672
                     /gene="vanY"
                     /locus_tag="IWI22_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9168471.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            40685..41494
                     /gene="vanW"
                     /locus_tag="IWI22_001065"
                     /pseudo
     CDS             40685..41494
                     /gene="vanW"
                     /locus_tag="IWI22_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT        11616 a         9542 c        10338 g         9998 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaat acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaatttt aggttatcgc tgaaagcccc ggaaatacgg
    13681 gcatttcgca ggatatgaga actcaattta ctaccaattt actactttcg gattgagccg
    13741 catatatgct gaaccatact gctaataccc gccaccccaa atacggcaca tcataaattg
    13801 aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat gggagaacag gacgccgctt
    13861 ttttatgccc aaatagagaa aggaggcgac gccatgctat ttacttccgg ccctgaccgg
    13921 gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc actacgacgg cggcggggcg
    13981 gacgcgccgg aggattgcgg cacctgccga ttttaccgcc cggattggaa atatcaattc
    14041 tgccagtttg cagagtgtcc ctatcagccg ggcaagctga ccgactatgc agcggtcaaa
    14101 ttcacagtga aaggagcgaa caacgctatg gcagttttcc gtgtggaaaa gaacaaaggc
    14161 tatacggtga tgtcaaatca ccacctgcgg aataagaacc tgaccttaaa ggccaagggc
    14221 ctgctgtcgc agatgttgtc cttgccggag gattgggact ataccctaaa aggactgtcc
    14281 tatatcaacc gggaaagcat cgacgcgatc aggacagcgg tatgggagct tgaaaaagct
    14341 ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca aaatggccga tatggtctat
    14401 accatctacg aacagccggt attggaaaac ccggtattgg aaaatccaac atcggataac
    14461 ccggtgttgg aaaatccgac atcggataat ccggcgtcgg aaaatccaac gcaattaaat
    14521 aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa taattacaga tggacaaagt
    14581 acccattcca ttcctatccc atccccgacc cctgccccta tggagggcga ggcagcggct
    14641 acgccgccgg aacggaaaag gacaggaaag gatgaggctg tacggattta tcgggaaatc
    14701 attttggaga acatcgagta tgcctatctc attcaggaca ggagcattga ccgtgaacag
    14761 cttgacgaaa tcgtagacct gatgctggaa accgtctgca ccgcccgaaa gacaatccgt
    14821 gtcgccgggg acgactaccc tgccgagctg gtgaaatcca agtacatgaa gctgaccggc
    14881 gaacatatcc gctttgtcct tgactgtctg cgggagaaca caacgaaagt ccgcaatatc
    14941 aaacaatacc tgcgggctat gctgttcaac gcccccagca ccatttccaa ctactacacg
    15001 gcccttgtcg cgcacgacat ggcacagccc gattggggaa agcctaaatc cggcctgccg
    15061 gactattcct gcggcgaggg cgaaagcctg taacgagaaa aggaggacga ccctatgaag
    15121 cagggcgcat tgatttttga cgagcgaaca gaccgttacg acatccgctt tgatctggcc
    15181 gactattacg gcggcctgca ttgcggggag tgtttcgacg tgatgatcgg cggcaaatgg
    15241 aggcccaccc gcattgaaat ggccgaaaac tggtatcttg tcggcatccg ggccgacgac
    15301 ctttccggcc tgcgggtgcg gatttgacct atatctacct gcggcgtctg gtggttccgc
    15361 cttagacgcc gcttttgact ttctgacaag gagggatgcg attgcaggaa gaagttactc
    15421 aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac ggctcaagtg ctgcaagcgg
    15481 cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa cacgctgcac cacggccagc
    15541 agagcctaaa gcagcttaaa aagcatgggg ctgatctctc caacattgag atcaccgagg
    15601 ccaacattgg agcgttcaag ccatgcgcca agaaatacgg cgtggatttt accttacgaa
    15661 aagataagac cacccagccg ccccactacg ttgtgatttt caaggccaag gatgcagata
    15721 accttgatct ggcgtttcgg gagtttaccg ccaagacgct ttccaaggag cagcggccct
    15781 ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc ggcccagacc aagcagcggg
    15841 ccaaggagaa aatcaaggaa agggggctgg aactgtgaag cctgacgtaa agaaattgct
    15901 gatcctgaac ctcccgtatc tgatttttgt gtacctgttt gcgaaatgcg gggaggcgta
    15961 ccggctggcc gctggtgctg acctgtcggg gaagctcctg cattttgcgg atggttttgc
    16021 ggcggctttt gccaacccgc tgccaagcct gcacctgttt gacctgtgta tcggtatcgc
    16081 cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc aagaacgtga agaaataccg
    16141 caaaggcatg gagtatggca gcgcccgctg ggggacggcc aaagacatag cgccttacat
    16201 cgaccccaag tttgaaaata atatcctgct cacccaaacg gaacggctta ccatgaccgg
    16261 caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg ctggtgatcg gtggcagcgg
    16321 ttcagggaaa acgagatttt tcgtgaaacc caacctgatg caatgctttc cgaccacgga
    16381 ctatccgact tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg aaaccggcag
    16441 gatgttccag caggcgggct accgcattaa aatcctgaat accattaact tttccaagtc
    16501 catgaagtac aacccctttg tctacatcca ttcggaaaag gacgtgctaa agctggtaaa
    16561 taccctgatt tccaacacca agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa
    16621 gtcggaacgg ttgttttatt cggccctgat cggctatatc tggtacgagg ccccagcgga
    16681 ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc agtgaagccc gcgaggacga
    16741 cccggagttt caaagccccg tcgatctcat gtttgagcgt ttggagcaga aagaccccga
    16801 ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc
    16861 catcctgatt tcatgcggtg ccagacttgc gccctttgac atccgggagg tacgcgagct
    16921 gatggaggac gacgaaatgg agctggacac catcggggac gaaaagacgg tgctgtatct
    16981 cattatgagc gatacggaca cgacctttaa tttcattctg gctatgctcc aaagccagct
    17041 aatcaacctg ctttgtgacc gggctgatga tgtgtacggc gggcggctgc ctgtccatgt
    17101 acgcctgatt ttggatgaat ttgccaacat tggccaaata cccaatttcg ataaactcat
    17161 tgccaccatt cgaagccgtg aaatatcggc ctctattatt ttgcagagcc agtcgcagtt
    17221 aaaagcgatt tacaaggacg ccgccgagat catttcggat aactgcgata gtgtgctttt
    17281 ccttagtggg cgcggcaaaa atgccaagga gatttccgat gcgctgggga aagaaacgat
    17341 tgacagtttc aacaccagcg agaaccgggg aagccaaacg tcccacggat tgaactatca
    17401 aaaattagga aaggagttga tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg
    17461 tatcttgcag ctacggggtg tgcgcccgtt tttatcggag aaattcgata ttaccaagca
    17521 tccccggtac aaataccttg ccgacgccga caagaagaat acctttgacg tggacagatt
    17581 tttgactatc acgcgccgga aacggcagca agtggtaacg caggatgaag tattcgacct
    17641 gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg cttttttcat
    17701 tccaacaaca aaatcaaaaa atcattatga gccggaaacc ggcagaaaga ggtaaattta
    17761 tggcattttt taattccgca gttactactt tgcagactat cgtgatcgga ctgggcggcg
    17821 ctctgtgcgt atggggcggc atcaatctgt tggagggata cgggcaggac aatccggggg
    17881 caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg cgtggccctg atcggtgtca
    17941 ctctggttcc cctgctgtcc gggctgctgg gctgatcctg gcctttgtcc ctgtccaaac
    18001 ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg agggcgcggg aaaggaggtg
    18061 ctacccgtga ttagtgaaat cattgaagaa tggataaaag gcatcctcat ggacggtatc
    18121 acgggcaacc tgtccgggct gttcgatacg gtaaatgcca aagtcggaga aatcgccgcc
    18181 gacgtgggac gaaccccgca ggcatggaac agcggtattt tcaatatgct gcacagcctg
    18241 tcggaaaccg tgatcgtccc catagccggg gccatactcg cccttgtcat gtgctatgag
    18301 cttatccaga tgataacgga aaagaacaat atgcacgact ttgacagctc catgttcttc
    18361 cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa cgaacacatg gaacatcgtc
    18421 atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa gcgccggtgt gattatcggg
    18481 cagacatcca ttgattttaa caccctgata ccagaccttg aaagccaatt ggaggctatg
    18541 agcgtgggcg ctcttttggg gctgtggttc caaacccttg ttgtgggcct caccatgaat
    18601 atcctttcca tctgcatttt ccttgtgacc tatggccgca tggtggaaat ctatgttgtc
    18661 acgtcgctgg ggcctatccc ccttgccaca atgggcaacg cggaatggcg cggcatgggc
    18721 caagggtatc tgaaatccct gctggcgctg ggcttccaag cctttttaat catggttgtg
    18781 gtgggtatct acgcggtgct gatacagaat atcggttccg cttccgacat atccggcgca
    18841 atctggggct gtatgggcta tacggtgctg ctgtgcttct gcctgttcaa gaccggcagt
    18901 atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc caagcataca caggaggaac
    18961 ttgtttattt caacgtacaa catcatttac gctgacccgc cgtggcggta tgactgcaaa
    19021 aacgtacagg gcgcagcgga aaatcactat tccaccatga gcattgacga gctgtgcgcc
    19081 ctgccggtgg aacggctggc gtccaaagac tgcctgctgt ttttgtgggc cacattcccc
    19141 atgctgccgg aagctctgcg gttaatcaaa gcgtggggct tttcattcaa gaccgtggct
    19201 tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt tttacgggct gggccgctgg
    19261 acaaggggaa acgcggaaat ctgcctgctg gcaacacacg gcaagccgaa gcgtcgttct
    19321 gcctctgtcc atcagtttat tatcagcccg attgaacaac acagcaaaaa gccggatgtg
    19381 acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc gcgtggagct gtttgccaga
    19441 cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg aatgtgacct tatcctgacg
    19501 tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag gacttaaccc atgtgaaaag
    19561 caaggttgtg tttgggctta ccaaacggca actgatctgt ttttccgggg cgctggtgat
    19621 cggcgtcccc ttatattttc tcacccgtga ctatcttacc aacagcgcgg cggccctgct
    19681 catggtattt gccatgctgc cgggcttttt gcttgccatg tacgagcgtc acggccagcc
    19741 ccttgaagtg gtgattatgc agatcatcca gtgctgcttc atccagccaa aggagcgtcc
    19801 ctatcagacc gacaacacct acaccgccct tgtgcggcaa taccagatgg aacaggaggt
    19861 aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga aaaaacaaag ctgacccgcg
    19921 cccagcgaaa agaaattgac gcggtaatcc gtaaatataa gggcgacggc aggccccaca
    19981 cggcgcagca gagcatcccc tacgaggtca tgtacccgga cggcgtgtgc cgggtatcgc
    20041 ccggcgtctt ttccaagtgc attgagtttg cggacatcag ctatcagctt gcccagccgg
    20101 acacgcagac ggctatcttt gaaaaactgt gcgacctgta taactatgtg gacgcctcca
    20161 ttcatatcca gttttctttc ctcaatcgga aagttgaccc ggtgcagtac gccaagagct
    20221 ttgagatcgc gccgcagggg gacgacttcg acgacatccg ggccgagtac accggcattc
    20281 tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa gaccaaatac ctgacattca
    20341 ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt gaagcgcatc ggctttgacc
    20401 tgttaggcta tttcaagagc atgggggccg tagcccatgt gatggacggc tgggaacggc
    20461 tgaacctgct gcatggcgtg taccacccgg acggcgagat attcaatttt gactggaagt
    20521 ggctggcccc gtccggcctc tccaccaagg attttattgc cccgtcctcc ctgtgcttcg
    20581 gcaatgcaaa gacattcggc atgggcggca aatacggcgc ggtaagtttc ctgcaaatcc
    20641 tgtccccgga gctgtcggac gatatgctgg ccgacttcct caatacggaa agcggtgttt
    20701 tagtcaatct ccatgtgcag gccattgaac agaccaaggc gatcaagacc atcaagcgca
    20761 aaattaccga ccttgacgct atgaagattg cggagcagaa aaaggccgtc cgcagcggtt
    20821 acgatatgga cattcttccc agcgaccttg ccacctacgg cgaggacgcc aagaagctgc
    20881 tgacaaagtt gcagacccgc aatgaacggc tgttccagtt gacgttcctt gtcctcaacg
    20941 tggccgacac caagcaaaag ctgaataacg acgtgttcca agccgcaggc gtggcccaaa
    21001 agcataactg tccccttgtc cggctggact accagcagga acagggcctt gcaagcagcc
    21061 tgccgctggg cgtcaatcag atcaagatac agcgcagcct tacgacttcc agcgtggccg
    21121 tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg cgcggccatg tattacggca
    21181 tcaacgccaa gtcccgcaac atgattatgc tggacaggaa gcaggcccgt tgccccaatg
    21241 ccttgaagct cggcaccccc ggcagcggca aatccatgag ctgtaaatcc gagatcgtca
    21301 gtgtgttctt aacgacgcct gacgatattt ttatttcaga cccggaggcc gaatattacc
    21361 cccttgtcaa acggctccac ggacaggtaa tccggctgtc gcctaccagc aaggactttg
    21421 tgaacccgct ggacatcaac ctgaattatt cggaggacga taacccgctg gccctgaaat
    21481 ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg caagaacggg ctggaagcca
    21541 ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta ccggccctat ctggcagacc
    21601 cccgcccgga gaatatgccg atcctgtccg acctccacaa agcgttgtta gatcagcatg
    21661 tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta tgtgtccggc tccctgaacg
    21721 tatttaacca ccgtaccaac gtggacatcg gcaaccggct tgtgtccttt gacattaagg
    21781 agctgggcaa gcagctcaaa aagctgggga tgctcattgt ccaagaccag atatgggggc
    21841 gcgtcacggc caaccgaagc caaggaaagg ccacatggta ttttgccgac gagttccatt
    21901 tgcttttgaa agaggaacag acggcggcct attctgcgga gatttggaag cggtttagga
    21961 aatggggcgg cattccaaca ggtgccaccc agaacgtcaa agacctgctt tcctccccgg
    22021 aaattgaaaa tatccttgaa aacagcgact tcattacgct gctcaatcag gcgtccgggg
    22081 atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga acagcagaag tacatcgaca
    22141 attccgaacc cggcgagggc cttttgatct ttgaaaacgt ggtgctgccc tttaccaacc
    22201 ccatcccgca caacacccag ctttacaaga ttatgacgac ccgcttaaac gaggtggcgg
    22261 gagtatgacc cggcgacgcc acaaaggagg tgaaccacca tgaaagagct gaaagccaag
    22321 gcgaaagtga cgcaggccat gacccgcgac gggctgacgc tggaaaatca ggccaccggc
    22381 gaggtggaga acatttccag ccgggaggcc gaacaggatt tgtccgcgcc ggggccgggc
    22441 ggcaccgccg ataagctgct ggaacgggcc gacgccatcc atgacggcca taaggccaag
    22501 aaagccgcga agcaggccgg ggctgtggcc gccgaggggg acgctgccct gcaccggcca
    22561 tccgcccggc tccaactgtc ggaggaagaa cgggccgacc cggatttaca gccctatatc
    22621 cgaaaatcgg acaaacgggc cgataagctg gataaggccc gcgccgccat ccccaagaag
    22681 cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg gcaaagcaaa gaccgtcctg
    22741 cactttgaca aacaggacaa ggggcctccc tccctgaaac ccaatccggc aggccgtcct
    22801 gtttccgagg tttttctatt cggccacggg aaaatccatg aagtcgagca tgaaaacgtg
    22861 ggtgtggagg gcggccataa gggggaggaa cttgccgagc ggcaggccgg aaaagccatc
    22921 cgcagcacca tccggcacca caggctgaaa ccctactggg acgcggaaaa ggccgagcgc
    22981 aggctattgt ctgccaacgc ggagtatttc taccagaaat ctttgcggga taacccggag
    23041 atcgcacagg cggccagcaa ccccatttcc cggttctggc agaaacagca gatcaagcgc
    23101 cagtacgcca aggccgcccg gacagcgggc cagaccgcag gcgcggcggc acagggcgcg
    23161 gccaagacag ccggggcctc cacgaaagcg gccaaggccg cagccaaaga aagtgaacgg
    23221 gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg ccgtccttgt gttggggctt
    23281 ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg ccatgttcgg cagcgccgga
    23341 agcggcatca cggcgtcctc ttacttttcc gaggatagcg atatgttggc cgccgaggac
    23401 gcctacgcgg agctggaagc cgagctgcaa gactatctgg acaactacga ggccacccat
    23461 gactatgacg aataccattt tgaccttgac gagatcagcc atgaccccta tgtgctgatt
    23521 tccattctgt cggcgctcca tgacgggata tttaccatcg gggaggtaca gggtgatttg
    23581 tccatgctct ttgacaagca atatatcctg acagaaaacg tcgtggtaga aacccgttac
    23641 cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca cccatacaga cacctaccaa
    23701 gtgccgtatg actattacat ctgctacgtc acgctggaaa acttcgacct gtcccatgtg
    23761 ccagtctata tcatggacga ggaaacgcta agtttctatt ccctgtatat gtccacgcta
    23821 ggaaaccggc ctgatctgtt ttccggcagc cagtacccca acgcctccac cctgacgccg
    23881 ccgacctact acgacgtacc cccggaggct ttggaggatg ccacctttgc cgccatgctc
    23941 aaagaggcgg aaaaatatat cggttatccc tatgtgtggg gcggcagctc tcccagcacc
    24001 agctttgatt gcagcggctt catttcgtgg gtaatcaatc atagtggatg gaacgtggga
    24061 cggctggggg cgcagggcct atataacatc tgcacccctg tttccagttc gcaggccaag
    24121 cccggcgacc ttgtattttt caagggaacc tacgacaccc ccggcgtgtc ccacgtcggc
    24181 ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc cgatttccta cacgaacctt
    24241 acttcaaact actggcaatc ccatttttac agttacgggc gtttacctta acgccagaaa
    24301 ggagctttca attatggcaa ccaaacttga ccgcatcgaa aaggacatcc agaaaaccaa
    24361 gtctaagatc gcggagtttc agaaacagct tcgggagctg gaaacgcaga aaaccgaaca
    24421 ggagaatttg cagatcatcc agcttgtgcg cggcatgaac atgaagccgg aggaatttgc
    24481 cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc gcaacccctt accatgaaca
    24541 ggaggacaat gcccatgaag aataagacca gacggatttc ccgtattctc cccgcacttc
    24601 tggccgtgat gctctgcgtc acggcctttc ccgttaccgc ccttgcaggc ggcaatgacc
    24661 cggcaccggc cccgctgccg gaggctacaa ccgaacccgc tacgggcggt ttggagcctg
    24721 aaacagacga aaccggcacc gccacggacg ggacagaaac cgaggccgaa aaagataccg
    24781 gccctctgac cggcaaggac atcaccgacc tgttttccgc gctgttcggt tccaaggtga
    24841 gcattgccgc cacggacgac ggcatccaga tcaccaccgg caccgacaaa aaagaacctg
    24901 aacagaccgg caccgtcacc accaacggcg gcaggctgaa tgtccgcacc ggcgcggggc
    24961 ttgacaagac cgcgttcacc cagcttccca atgggacgac ggttgaggta gtcggcacag
    25021 acggcgactg gattaaaatt ctcttgccgg agcgcatcgg ctatgtccat tccgactata
    25081 tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg cggtttttcc ctctccattg
    25141 acccggagga aattgcttct ctgctggaac tgttcaatgg cggcggggcc agcgccgccc
    25201 tgtccccgga cgggaacctg tcactgattg acgacatcgg cagcgccgcg aaaaatggca
    25261 agcagtttat caccgtggaa accaaaaacg gcaatgtgtt ttatctgatt attgaccggg
    25321 acgacgaggg aaaagaaact gtccatttct taaatcaggt ggatgaagcc gatctgctga
    25381 cgctgatgga ggacggggaa accgtcaccc ccgctgctgt ctgctcctgt accacgaaat
    25441 gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa aaccaacctg accgagtgca
    25501 gcggcccgga gccgcaggaa ccccagccgg aggaacccga agccccggag gaagaaccca
    25561 agggcggcgc gggcggcctg atcgtgttcc tgctggtggc gctggcgggc ggcggtgcgg
    25621 ccctttacta tttcaagttc aaaaagccca aggctgaaac caccggcagc gacgatttgg
    25681 gcgagtatga ttttggggag gacgaggatt tggaggacga ggaacccgaa accgagtttg
    25741 acccggaagc tgattttgtg agtgaacagg aggacgagga atgacatata aacttgtaat
    25801 cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg atcggggcca cggagaaaca
    25861 cgacggctat ttggagggga acggctatct ggtgagctgg tgcatcggac atctggtgtc
    25921 ctttgccgac gccggacgct atgacgagcg tttcaaaaaa tggcgctatg aggatttgcc
    25981 cattctcccg gaaacatggc agtacatcgt ccccgacgac aagaaacagc agtttgacgt
    26041 tctctgttcg ctgatgcagc gccccgacgt gaccggcctt gtgtgcgcca ccgacgccgg
    26101 gcgcgagggg gaattgattt tccgtttcgt ctaccagatg gcgggatgcc aaaagccctt
    26161 tgagcgcctt tggatttcct ctatggagga cagtgctatt aaggacggct ttgcccacct
    26221 gaaaccgggc ggcgactatg atccgctgta tcaatcggcc ctgtgccggg ccaaggccga
    26281 ctggctgatc ggcattaacg ccacccggct tttttccgtc ctgtaccaca agaacctgac
    26341 tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg gacagggacg ccaagattac
    26401 cgatttcaag aaagagaaat atcacatcgt ccatatcacg gcgggcggcg cggacgcggt
    26461 aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc aaggcggctt gtgcaaaagc
    26521 gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg gaacagccgc ccaagctcta
    26581 tgaccttacc accttgcagc gggaggctaa ccggcttttc ggctataccg ccaagcagac
    26641 ccttgactat gcccaggcac tgtatctaaa gaagctgctg acctatccga gaacggatag
    26701 tcaatatctt accgacgata tgctgcccgc cgtggaaagc cttgtgtccg gcctgtggga
    26761 gctggtgccg tttgccaagg gcctgaacct ttccccgcag tttgaccgca tcctgaacag
    26821 caaaaaggtg tccgaccacc acgccattat ccccaccgct gaatttgtga agcagggctt
    26881 tggcactctg gccgagagtg aaaaaaagct gctgtccctg atctgctgca agctcttatg
    26941 tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc acatttacct gtgccgggca
    27001 ggagtttacc gccaagggca agaccgtcct ttcccccggc tggaaagaga ttgaacgccg
    27061 gttccgttct tccttaaaga ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc
    27121 ggagctttgc gagggccaga cctttgcaga cgtggccgcc tctgtcaccg agcatttcac
    27181 cacgccgccc aagccctaca ccgaagatac gcttttgtcc gctatggaac gggccggggc
    27241 cgaggatatg ccggaggatg ccgagcgcaa gggattaggc acaccggcca cccgcgccgc
    27301 cattctggaa aagctggtgc agatgggctt tgtccagaga aaaggcaagc agctcattcc
    27361 caccaaggac ggcatcaatc tggctgtggt gctgccggag gcgcttactt ctccccagct
    27421 tactgccgaa tgggaaaacc ggctgaccga gatcgccaag gggcaggccg atcctgacga
    27481 atttatggac ggcatcgaga cgcaggcgcg ggagctggtg cagacctatt cctgtatcag
    27541 tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct atcgggacgt gtccccgctg
    27601 tggtgaggct gtctatgagg gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt
    27661 cgtcatgtgg aagaatgacc gattttttga ggaacgcaag aaagcgttca ccccgaagat
    27721 cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac
    27781 cggcaaaacc tacgacggga cggtgctttt ggctgatacc gggggcaagt acgtcaacta
    27841 ccgcattgaa cacaggagct aaaggacagt aacacagcaa gcatagggag tgtggctaca
    27901 ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg ggatttttgc ttgttgggga
    27961 agtccccaac cccctatttt ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag
    28021 tattttctat ccaagtccat aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc
    28081 tggatttgcc caccacaacc gaaacgctgc aagccgccct gcgggatgtt ggcattactg
    28141 ccgacaatcc gcaggatttt tttatcaacg ggtattcctg cccggaggac agacaccttg
    28201 ccattcccta tgacatggtg ctggccgccg acgtggacga gctgaatttc cttgccgccc
    28261 ggctgggcca gcttgacgcc gccgagatcg cagagctgaa cgccgccctg caaaatcccc
    28321 gtggcggctt tgagaatatc ggccagatca tcgactacac cgaaaatgtg gattactatg
    28381 tgcatcttcc cgacgttagg ggggatggac agttgggcga ctactacctg aaccgttccg
    28441 gcatggtgga tatgccggag gaatggaaag ccgggattga tacggcgcag tttggacgcc
    28501 acattgccca gcaggaacaa ggcgttttta cccagtacgg ctatctggtg agaagcggcg
    28561 acgaatggca gcgcgtccat gagggccagc cggtgccgga ggaataccgg gtgatgcact
    28621 acccccagcc ggaagctgac cgggaggaac cgcaggccca gcagccggag gcatccgaaa
    28681 gccccgacaa agcgccgccc ccggttatcc cgatcatgct ggacggcatg aacagcaaag
    28741 aacgcatgaa agtgattact gaccgtctgg aaaccggcgt acaggagctt tttgaaagta
    28801 gccgctatat ggcttactta tccgctatgt ccaagatgca cgattacagt ctgaacaata
    28861 cgctgctgat cgccatgcag ggcggttcga ttgtggcggg ctataacagg tggcaggatg
    28921 aatttgaccg ccatgtgaag cggggcgaga aaggcattaa gatactggcc ccggccccgt
    28981 tcaaggttaa gcagcagatg gagaaaatcg acccggccac ccagcagccc gtgatcggcg
    29041 cggacggcaa gcccgtgacc gaagaacagg aagtgacggt tccggccttt aaggtggtga
    29101 cagtctttga catcagccag accgagggca aggattttcc catgccctat atcagtgaac
    29161 ttaccggcga cgtgcagcag tatcaggatt tctttgccgc gctggaacgg acatcaccct
    29221 atcccataag cattgagccg atttcagacg gggccaaggg ccgctgcttc tacgcaaaac
    29281 agcgcattgc catcaacgag ggcatgagcg aattgcagac cctaaagacc gccatccatg
    29341 agattgccca tgccaagctc cacgccattg accgggaggc ccccgccgag gaacaggcgg
    29401 cccggccaga ccgcagcacc cgcgaggtac aggccgaaag cgtggcctat accgtctgcc
    29461 agtattacgg gctggacacg tcggactatt cttttggcta tgtggccgat tggagcagcg
    29521 gcagggagtt atccgagctg aaagcctccc ttgaaaccat ccgggccacg gccagcgaat
    29581 tgataaccga gatcgacggc cattttgccc agcttcagca ggagcgcgag gcccggcagg
    29641 aacaggaggc cgtcacggtt gacgttaccc cggaggcagc ggccccggcg caggccgagg
    29701 aagaacaggc cccggccctt gatccggcag ccgagccgct tgtgactgtg ctatggagcg
    29761 aaagccccca gctaaaggac ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg
    29821 cccgtctgga tgcagcatat ccggcagagg caggctatga caaaaccgcg ttccgcattg
    29881 actgtatctt tcagggcgag ccggacagct acgaggggcg gcaggatttt ggagataggg
    29941 acggttccct gatcgaacat atccagagct accaccaatt ttacgcgcag gacgaaaact
    30001 ggaaaaagca tgtaatcgct acaaccggca tagagggctg ggagcaggac aaggcccatc
    30061 gggatatgct gttaggcgag tttgtcccct atatgaagct gcattgcagc cttgcccaaa
    30121 tggaacagac ggcgcagggc cgcttacagt ccggggaaac cctgacgccg gaggaaacgg
    30181 cctatttggg ggcgcttctc tcttatgtgc aggagtgccg ccccatgctc aatcagggcc
    30241 agtatcagtt gccggagccg ccgaaactgg ctgacttcga ccagagcttg caggactaca
    30301 aagcacaggt acaggccgag atcgcgcagg aggccgccgc agccggtatg accgtggagg
    30361 aatacgcggc ggctggctat gaagcacccc aacaggacac cttttccatc taccagcttc
    30421 gaggcgggga ggaaacccgc gactatcggt ttgagccata cgagcgttta caggccgcag
    30481 ggctggccgt ggacagtgcc aactatgacc ttgtttatac ggcaccgctg gataaaagca
    30541 cgtctttgga ggatatttac cgcacattca acatcgacca cccggcagat tttaggggac
    30601 acagcctttc cgtttccgac gtggtggtgc tgcaccagaa tggacaggac accgcccact
    30661 atgtagacag cttcggctat atggaagtgc cggagttttt gcgggaaaat cccctgcgta
    30721 ccgccgaact gtctacggag cagaacgaaa acatgattga cggcattatc aacaatacgc
    30781 cgcccgaacc gcagccggag gaacccgaac agctatcttt tgaggatgtg gcagttgctt
    30841 ccggccagac acaggaggaa cccccggagg ctgcgccccc gcaaacggct gtccgctatt
    30901 atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc catgagcttt tctgactacc
    30961 ggcccggcag cgccacggcg gaataccggc actatgtcga tcaggccgtg gaaattgcgg
    31021 agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa gatagacagc ctccttgaca
    31081 cctacgcccg caagctggcc gagaacatga ataagggcta tgagattacc gcccgcgtcc
    31141 cctctgtcct gattgccggg ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg
    31201 ccgccagtga ccgcaacatg gaggaatttc agtacattca gggcttgctt gacaaaatcc
    31261 gcagcaccgg catgggtggt atcagcgccg acgatccgca ggccgtttcc aagctggaaa
    31321 agaaactgga aaagctggaa gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc
    31381 gcaagcatgg gacgctggac ggatgccccc atctgaccga aagaggcatt gaaaacctga
    31441 aagcggatat ggcgtcgggc tggcactatg agaaaaaacc gttccagtcc tggcagctct
    31501 ccaacaataa cgccgagata cgccggttaa agggccgcat tgaggaattg acccgccaaa
    31561 aggaggcggc ctatgtggga tgggagtttg acggcggcac tgtggagatc aaccgggtgg
    31621 ccaaccgcct gcaaatcttc tttgagggga agccggatgc tgctgtacgc gacgagctga
    31681 aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca
    31741 ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc cctttccggc gaaaagccca
    31801 gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc ctctatccgg gcgcagctca
    31861 acgcggccaa gaccgaacag gccaagcagc caccggcgcg ggaaaaatca aagggattgg
    31921 aggtagagta aatgcagaag tttgaaaatg tggacgttct ggccgcgctg gaacagatca
    31981 tgcggcagaa caccgcgttt tatcaaagtg actttggcat tgacaaagac attatcaggc
    32041 gggatgccgc cagcgatcag gccgcagaca agaccctgct ctggatgtcc cggccctccg
    32101 ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga cacccgacag tacaacacat
    32161 ggaaatttta cggggagcag acccgtgaca agatactggc ctacgcggtg gagctgaccg
    32221 gcacacaggg cggcactatc cggggcaatc tctatgagct ggactatccg cagcatttcc
    32281 ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg gctcttttat gagcatggca
    32341 cccgtgacat cccggagagc cagcgttttg acggaagccc tgaccgggta ttgggaaact
    32401 ttttgtacta tgaggcccag ccccatgacc cggctgtgct gcaggaggcg ctacggacgg
    32461 agcagcacag ccgggggcgg gccgtccccg gcgatttcaa agtccacgtc accgctttgc
    32521 atgacagccg gattgaaacc gaggcccggc gtattgtgga cagcctgaaa gatctgtccg
    32581 cgcctaacag ccccaataaa acgcatttca tggtggagat ctccccgcac tttgcggcga
    32641 ttgcttccac caaggataat gaccggctct ttgccatgct gccctataaa tccctgtgtt
    32701 ttaccgggat gaaaggcagg catggcgtgt atgccgtgat taaccgggac gagcgccggg
    32761 atgtgagtat tcgcaggccc cggccctcta tccgcaagca gctatccgac agcaaacagg
    32821 ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa tgaattggag gtataaggaa
    32881 tgatgcactt taccgtggag gaagaaaacc tgatctgtat gtaccacaat gcagaccggc
    32941 gcagaacgat aaacaggctc accgccgtca tgccggttat ggatggggat atgcgggcgc
    33001 aggcccagca gaccctttcc aagctggaac gcatgacgga tgccgactat gacggccaga
    33061 agttccactt cacagacgaa taacgaacag cagacgccgg gcgcggcggc catgtggtcg
    33121 cttgcgtccg gcgtcttttc tttttgccta ttcctacgca tttagaaccc ggtattgtcg
    33181 gggataggat gaagtattcg cttgcccctg caaacatgcg ggagaagcct tgcagcgggc
    33241 ggttttcaat cccttaaatg cgtaggttgc agctataatt agacccctga aacagatata
    33301 gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa
    33361 gagggtgcag ccctcttgcc gtctatctct gttccagcgg gaagctcggc aatgcttcca
    33421 gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat gactttcttc tttttgccgt
    33481 ttgcctgtct gatctccacc gtggaaagct cgttgatgta gtcagcataa tgggccaaca
    33541 ctttttccgt ggcccacctc tcaccggcaa cggcggcttt gatcgtttca tagtccagca
    33601 gcttttcttg tccgtccatc ctgatacccc cgtcagactg attttttcta tgataccata
    33661 ttcggaagca tggaaccaga gaaaacggcg cttttctgct ggtaaattct acgcatttag
    33721 aacagcactt cggcggggat aggataaaac acccacatac cccgctgacc atgccggaaa
    33781 agccttgaaa tataagggct tttacagctc taagtgcgta ggtttccagt atcttttccg
    33841 ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag caggccgcgc agtatttggc
    33901 ccggttagac ttcggcgtga acgtggcccc gcactcacag cagcgtttca cttcgtcccg
    33961 gtctttggtg atctcggcat acagggccgc atccagcggg aggacagcgg ccttaaacca
    34021 gcggcacaga agcgaatagg aaatgctctg tacgcaaata cattcttcgc cgtcgtccag
    34081 caacaggcag ttgccgtcac aatagttgca gcactcatgc gtcagcttcc gggccttgcg
    34141 atattgaggg taattcattc gtggaatact catagctctt gcgtccattc ctttcccggc
    34201 gtcatacgca aaatgccgtc cacattctgc ttgatgatgc cgtattcctg catctgcttt
    34261 ttcgcttcgc cgtacttctc atacagccgt tccttttcct cggccaatcg cttgtactcg
    34321 gctttcaggg cgtacagatc ggggagcttt ttcacaccct gtccctttag ggcctttgcc
    34381 gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc ggaattttgc cttatccgct
    34441 gcattgcggt attccatagc cacgggccag agctgcttgt aggtagaaat attctttatc
    34501 agcaccgcca tatccgcaag ccggtgttcc atagctttga gggtagcggc ggcctcgtcg
    34561 ctggcggcgg tgatctcggc cactttgctt tccaaatccg ggtaggtgtc tatcccatgt
    34621 tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc
    34681 tcatagcctg ccgactgctg ggccttaatg ttgttttcca gatcaatccg caaagaaatc
    34741 cccttgcgct ctttgggcgc tctggtgata acgcggcctt tgatacgctc tttgatggct
    34801 tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa agcgttcctg tccgggagcg
    34861 cggaaagaaa taaactttcc cggcttgatc tcatagccct gcgcctgcat gagccgcagg
    34921 aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct
    34981 ttccagctcg tccctttccg ctgggcgtcc cactcggcat agctcttgcc cttactctgg
    35041 ccgggcttca ccacggacaa tccgttttcc ttgcacagcc tgtcgctggt gcgccggata
    35101 taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat ttaccatatc cacggcacag
    35161 aaaataatgt gattgtggac gtggccctta tcaatatggg tagtcagcac atagggatat
    35221 ttcccttgca gcacttcctc ggcaagctgc ttcccgatct catgggcctg ttccggcgtg
    35281 gcttcgccgg gggcaaagga ttgtatcagg tggtggccca gattattgcc cttttggtat
    35341 gcctgcgcca gcagcatttc aaattcaatg tccgctgttt cataagagca ggcaaaggag
    35401 gccaccagca gcttgccgtc agttttgtcc gggttctcga tgtagtcaag ggcttttttc
    35461 agcgttgact tgataggatg tatctttgta actgccatat ctgcgccagc gcccccttta
    35521 tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt cagcctcttt gcaatctgat
    35581 tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca
    35641 tgttgacgat atagccgtca atgagcatct tccgggcgta ggctgaaaag ttcttcgtat
    35701 gcagcagcgc cattttctgt tcaatcatag cccgttcctc cggcgtcaca gggacgcgca
    35761 gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc tttccattca ggggtttggg
    35821 gatttcccca acaagcaaaa cggacgcggc ccggcaggga cggggcaggc cgtttttccg
    35881 ggagtggcta cactccctat gcttgctacg ttactgtttc tccactaaca agacgtttcc
    35941 aaagccccaa actacccgca aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa
    36001 gctacgctgt gcagctcccg ctaaaagtaa aacagaggat gccagttaca catcctccgt
    36061 ttcattcttc ttcggctttt caattagtat ggcgctgtca atcgcgccct cgatgatcgg
    36121 caggtattct tcggggcaga gtttcagctt gtggctgacc ctttgccgca gttcgctttc
    36181 ctcccgcatg atctccgggt taaagtagcg ttccacagga agcccgcagg ctttaataag
    36241 ctgtatgaca accggcagac tgggaatggc cccgtcgttt tcgatgttgg caagatagcg
    36301 ccagtcaata tttattttct ctgccattgc tttgcgcgaa acgcgctttg cttgccgggc
    36361 cgctttcaca tccgcgccga aagtttcaaa gccgggacaa tcttcaatat tcgccatatc
    36421 gcacctccca cttacattgt atatttcata ctctgcccgt ggaatgttgg tatatgcacc
    36481 tttcttaaag tttatatttc acattttgtt tcttttattc ttcgagcaaa agaattataa
    36541 tgtaccctgt ggaggtgtac tatggactac atgacgttga aagaggcagg cgagaaatgg
    36601 ggtgtgcccc cacgatggat aaactactat tgtgctgcgg gacgcatccc cggcgctgta
    36661 aagatggcga cgatctggct cattcccaaa actgcggaaa agccgattga caggcgtaca
    36721 aagaaaggaa aggcggttac tgaatagtat aatgaaaccg gcgcattttg gatatggttt
    36781 tgaagagaga gaaagcatgg tattttgtca cggaaaaact ataagatgat agatggcagg
    36841 ctgctccaga ccgataaaaa gtattctcat ctgaaacaga aagaaaagat ttacacatgg
    36901 atgtatgaag aagcgaaact ttatcatgac agaaatggaa aatgcccgaa acaaagacga
    36961 ggaagctgct gctgtatcag ccgtatatgg tagaattgaa aaggccggca tctggattct
    37021 ttatggtgaa gtctccagac attggataac tgctggtcta atgcgccttt gagtatgggg
    37081 tgattgattt taataccggg gaggtacggc tttttgagga aaatattctg ggatttacat
    37141 ttggcgatta ttacatttcc tgtaactgca aggacacgga tgatgaagat atgaggaata
    37201 tagacttgct gattttctac tgtcttgaac ggtattagga ctgaatctgc tgcgcggagt
    37261 gcggcaagag cgaccggagg gagtacacac aggccctgcc tgtgtgaaaa gccataccgc
    37321 aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag gcttggggtg tccccaacaa
    37381 gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag aatatccgta aaggatattc
    37441 tgatccattg cttgccagta ttatcgggat gttttgacaa ccgctgtaat cgaaacatcc
    37501 caaacattaa gaaggtgtta attattatta tcttgattta attcgtttaa accaataata
    37561 tagatacata caccatgaaa agaagcgagt atcccaatga cggatgatta aaatccgaaa
    37621 agaaatcgct ttgaaactac agggaaacta cagactgtta tgttatcttc ttaaaatgga
    37681 gggattttta tgtcgatacg aattctactt gtcgaggatg atgatcatat ctgtaataca
    37741 gtaagggcgt ttctggctga ggcaggatat caggtggatg cctgcacaga tggaaatgag
    37801 gcatacacca agttttacga aaacacttat caactggtta ttcttgatat tatgctgccc
    37861 ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga aaaatgatac tcccattctg
    37921 atgatgacag ccctgtcgga tgacgaaaac caaatccggg cgtttgatct agaggcagac
    37981 gactatgtaa caaagccatt caagatgcag attttactaa agcgggtgga agccctgctg
    38041 cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca ggctgacact tctgccggag
    38101 gatttcacgg tactttgtga cggtacagag ctgcccctga cacgaaaaga atttgaaatc
    38161 cttttgctgc tggtgcagaa caaaggcaga accttaacgc atgaaatcat tttgtcacgt
    38221 atatggggat atgactttga gagtgatggc agcacagtcc acactcatat caaaaatttg
    38281 cgggccaagc tgccggaaaa tatcatcaaa accattcgcg gtgtaggata ccgattggag
    38341 gaatcataat aatggaaaga aaagggattt tcattaaggt tttttcctat acgatcattg
    38401 tcctgctact gcttgtcggt gtaacagcaa cactgtttgc acagcaattt gtgtcttatt
    38461 tcagagtgat ggaattacag caaacagtaa aatcctatca gccattggtg gaactgattc
    38521 agaatagcga taggcttgat attcaagagg tggcagggct gtttcactac aataaccaat
    38581 cctttgagtt ttatattgaa gataaagagg gaagcgtact ctatgccaca ccaaatgcca
    38641 atacatcaaa tagttttaga cccgactttc tttatgtggt acatagagat gataatattt
    38701 cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta tcaagggctg acaattcggg
    38761 gaattgttat gattgcgata atggttgtat tcagcctttt atgcgcgtat atctttgcgc
    38821 ggcaaatgac aacgccgatc aaagccttag cggacagtgc gaataaaatg gcaaacctga
    38881 aagatgtacc gccgccgctg gagcgaaagg atgagcttgg cgcactggct catgatatgc
    38941 attccatgta tgtcaggctg aaagaaacca tcgcaaggct ggaggatgaa atcgcaaggg
    39001 aacatgagtt ggaggaaaca cagcgatatt tctttgcggc agcttctcat gagctaaaaa
    39061 cgcccatcgc ggctacaagc gttctgttgg agggaatgct tgaaaatatc ggtgactaca
    39121 aagatcattc taagtatctg cgcgaatgca tcaaaatgat ggataggcag ggcaaaatca
    39181 tttccgaaat actggagctt gtcagcctga atgatgggag aatcgtaccc atagctgaac
    39241 cgttggacat agggcgcacg gttgccgagt tgctgcccga ttttcaaacc ttggcagagg
    39301 caaacaacca gcggttcgtc acagatattc cagccgggca aattgtcctg tccgatccga
    39361 ggctgctcca aaaggcacta tccaatgtca tattgaatgc agttcagaac acgccgcagg
    39421 gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa atgccgcctt tttgttttga
    39481 acatgggcgt tcacattgat gatactgcgc ttccaaggct gttcacccca ttctatcgca
    39541 ttgatcaggc gcgaagcaga aaaagtgggc gaaacggttt aggacttgcc atcgtacaaa
    39601 aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt
    39661 tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttga
    39721 ccgacaggta taaccctgcc ggtctttttt tgctgctaca ggaaaactac agactggcta
    39781 cagggaaagt acagatgcgc ttgccataat aacaatcgta ccagccacaa atcgtagttt
    39841 tattgcaaag gaggcattcg atcaaatgga aaaaagcaac tatcattcca atgcggatca
    39901 tcacaaacgg catatgaaac aatccgtgga aaagcgggct tttctatgtg cgttcattat
    39961 ttcgttcaca gtctgcacgc tgtttttggg gcggagactg gcttccgtat tggaggcaac
    40021 acagataccg cccatccctg caactcatac aggcagcagc actgacgtag tggagaattt
    40081 ggaggaaaac gctcttgcca ccgccaaaga acagggagat gaacaggaat ggagcctgat
    40141 tttagtgaac aggcagaacc ccatccccgc acagtacgat gtggagcttg agcaactatc
    40201 aaatggtgag cggatagata ttcggatttc tccctatctt caagatttgt ttgatgccgc
    40261 aagaactgat ggagtttacc cgattgtcgc atccggatac cgaacaacag aaaaacagca
    40321 agaaattatg gatgaaaaaa ttgccgaata taaggcgaaa ggctacacct ctgcacaggc
    40381 taaagcggaa gcagaaactt gggtggccgt gccgggaacg agcgagcatc agcttggtct
    40441 tgctgtggat atcaatgcgg acggaattca ttcaacaggc aacgaggttt atagatggct
    40501 ggatgaaaac agctatcgtt ttggttttat tcgtcgctac ccaccagaca agacagagat
    40561 aaccggtgtg agcaacgagc cgtggcatta ccgatatgtt ggcatcgaag ctgccacaga
    40621 gatgtacaac caaggggttt gccttgagga atatttaaaa ccagaaaaat gagaaaagga
    40681 tgttatggac agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc
    40741 gcagagaaaa atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac
    40801 gataggggaa aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca
    40861 caataccgga tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc
    40921 aaagacctta aacggcttat tgataagacc gggggaaacc ttttctttct ggtggctggt
    40981 acgccatgcg gacaaagata ccccctataa agacggcctt acggtgacca atggtaaact
    41041 caccaccatg tcgggcggcg gtatgtgcca gatgagcaat ttactatttt ggatgttcct
    41101 gcatacgcca ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc
    41161 aaacagtgac gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa
    41221 agtgcgaaac gataccgact gcacctacca aatatgagtg accttagatg atgagaaaat
    41281 catcggtcag gtgtccgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag
    41341 tatccagtat gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca
    41401 agttgcctta ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa
    41461 aatctgttat cccctcccgg aaagtgtgga tatt
//
LOCUS       DACDNO010000019        40408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDNO010000019 DACDNO010000000
VERSION     DACDNO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.019"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..411
                     /locus_tag="IWI22_001066"
     CDS             4..411
                     /locus_tag="IWI22_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168472.1"
                     /translation="MLSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELTPLDRN
                     GIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEK
                     RNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            421..624
                     /locus_tag="IWI22_001067"
     CDS             421..624
                     /locus_tag="IWI22_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168473.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            835..1419
                     /locus_tag="IWI22_001068"
     CDS             835..1419
                     /locus_tag="IWI22_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9168474.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1790..3115
                     /locus_tag="IWI22_001069"
     CDS             1790..3115
                     /locus_tag="IWI22_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9168475.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            3108..3458
                     /locus_tag="IWI22_001070"
     CDS             3108..3458
                     /locus_tag="IWI22_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168476.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3455..3634
                     /locus_tag="IWI22_001071"
     CDS             3455..3634
                     /locus_tag="IWI22_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168477.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            3770..4060
                     /locus_tag="IWI22_001072"
     CDS             3770..4060
                     /locus_tag="IWI22_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9168478.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            4428..5216
                     /locus_tag="IWI22_001073"
     CDS             4428..5216
                     /locus_tag="IWI22_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9168479.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            5203..5529
                     /locus_tag="IWI22_001074"
     CDS             5203..5529
                     /locus_tag="IWI22_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168480.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            5878..6918
                     /locus_tag="IWI22_001075"
     CDS             5878..6918
                     /locus_tag="IWI22_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9168481.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            7754..8086
                     /locus_tag="IWI22_001076"
     CDS             7754..8086
                     /locus_tag="IWI22_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9168482.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            8677..9006
                     /locus_tag="IWI22_001077"
     CDS             8677..9006
                     /locus_tag="IWI22_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168483.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            8993..9334
                     /locus_tag="IWI22_001078"
     CDS             8993..9334
                     /locus_tag="IWI22_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9168484.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            9448..9687
                     /locus_tag="IWI22_001079"
     CDS             9448..9687
                     /locus_tag="IWI22_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168485.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            9754..9993
                     /locus_tag="IWI22_001080"
     CDS             9754..9993
                     /locus_tag="IWI22_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168486.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            10079..10750
                     /locus_tag="IWI22_001081"
     CDS             10079..10750
                     /locus_tag="IWI22_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9168487.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            10803..12779
                     /locus_tag="IWI22_001082"
     CDS             10803..12779
                     /locus_tag="IWI22_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9168488.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            12792..13448
                     /locus_tag="IWI22_001083"
     CDS             12792..13448
                     /locus_tag="IWI22_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9168489.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            13544..14371
                     /locus_tag="IWI22_001084"
     CDS             13544..14371
                     /locus_tag="IWI22_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168490.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            14381..14629
                     /locus_tag="IWI22_001085"
     CDS             14381..14629
                     /locus_tag="IWI22_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168491.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            14660..16771
                     /locus_tag="IWI22_001086"
     CDS             14660..16771
                     /locus_tag="IWI22_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9168492.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(16809..18866)
                     /locus_tag="IWI22_001087"
     CDS             complement(16809..18866)
                     /locus_tag="IWI22_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9168493.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(18980..19192)
                     /locus_tag="IWI22_001088"
     CDS             complement(18980..19192)
                     /locus_tag="IWI22_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168494.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            19379..19846
                     /locus_tag="IWI22_001089"
     CDS             19379..19846
                     /locus_tag="IWI22_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168495.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            19843..20085
                     /locus_tag="IWI22_001090"
     CDS             19843..20085
                     /locus_tag="IWI22_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168496.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            20103..20405
                     /locus_tag="IWI22_001091"
     CDS             20103..20405
                     /locus_tag="IWI22_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168497.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            20476..22530
                     /locus_tag="IWI22_001092"
     CDS             20476..22530
                     /locus_tag="IWI22_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168498.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            22530..24362
                     /locus_tag="IWI22_001093"
     CDS             22530..24362
                     /locus_tag="IWI22_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9168499.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            25057..26973
                     /gene="ltrA"
                     /locus_tag="IWI22_001094"
     CDS             25057..26973
                     /gene="ltrA"
                     /locus_tag="IWI22_001094"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9168500.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            27090..27890
                     /locus_tag="IWI22_001095"
                     /pseudo
     CDS             27090..27890
                     /locus_tag="IWI22_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            27887..30418
                     /locus_tag="IWI22_001096"
     CDS             27887..30418
                     /locus_tag="IWI22_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168501.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            30468..30800
                     /locus_tag="IWI22_001097"
     CDS             30468..30800
                     /locus_tag="IWI22_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168502.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            30801..31394
                     /locus_tag="IWI22_001098"
     CDS             30801..31394
                     /locus_tag="IWI22_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168503.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            31557..33581
                     /locus_tag="IWI22_001099"
     CDS             31557..33581
                     /locus_tag="IWI22_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9168504.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            33581..34786
                     /locus_tag="IWI22_001100"
     CDS             33581..34786
                     /locus_tag="IWI22_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9168505.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            34802..35446
                     /locus_tag="IWI22_001101"
     CDS             34802..35446
                     /locus_tag="IWI22_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168506.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            35457..36263
                     /locus_tag="IWI22_001102"
     CDS             35457..36263
                     /locus_tag="IWI22_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168507.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            36285..36515
                     /locus_tag="IWI22_001103"
     CDS             36285..36515
                     /locus_tag="IWI22_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168508.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            37061..37336
                     /locus_tag="IWI22_001104"
     CDS             37061..37336
                     /locus_tag="IWI22_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168509.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            37378..37800
                     /locus_tag="IWI22_001105"
     CDS             37378..37800
                     /locus_tag="IWI22_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168510.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            37819..39411
                     /locus_tag="IWI22_001106"
     CDS             37819..39411
                     /locus_tag="IWI22_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168511.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            39423..39758
                     /locus_tag="IWI22_001107"
     CDS             39423..39758
                     /locus_tag="IWI22_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168512.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            39905..40099
                     /locus_tag="IWI22_001108"
     CDS             39905..40099
                     /locus_tag="IWI22_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168513.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            40131..>40408
                     /locus_tag="IWI22_001109"
     CDS             40131..>40408
                     /locus_tag="IWI22_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168514.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMG"
BASE COUNT        14854 a         6174 c         8022 g        11358 t
ORIGIN      
        1 tttattttat ctttaggtat tttaactatt ggaattagtg taactggaac tacaattcct
       61 acatttgctg atagtacaaa tgaaccaact actgtagtag ataataacca agaattaact
      121 cctttagata ggaacggaat agccattggt aacccaacta acgacttaac tgttactgaa
      181 aattatttaa tagtttctcc attacttgct cgagcagctt ctaaaacgat acgagtgaca
      241 ggaacgatga tttttgataa agttggttgg ccttctagac ctcctcaatc acggaattgg
      301 aaagaaaaaa gaaatggtta ttggtactat ggaaaattgt atttacaaag ttataaatca
      361 gctggaaaca attggattgc gacttattca ggaaccttaa ctagaaagta gggatttttt
      421 atgccagaat ttgtgaacac accaaatttt tatcaagata gcgatattct aagtaaaatt
      481 aatttaccga aagaagaatc agctttacaa gttcagcgaa ctttacagga gacacaaaaa
      541 attcaaaaat caatttctaa tctagaagat tctgaagaaa aaagaaaaaa aagagaccaa
      601 aaaacatatc aaaaagaaaa ataatgattt taaaaaaaga gtgtctagtc gacattcttt
      661 ttttctaatt gaaagtgttc ataaaatcac ccatttctgg acacttcctt tttttgcttt
      721 atttttcaaa aaaacaattc atttcatgtt cataaaccca taacaatatc tatgttcagt
      781 taaactatat ggataaagtt atgttacaat aaaattaatg aaggagtgat ttttttgaaa
      841 ataggatacg cccgggtatc tactggttta caaaatttgg atttacaaga agacagccta
      901 aataaatttg gttgtgaaaa aatttttact gatcatatga gtggggcaaa aagtaatcga
      961 ccaggattag aaatggccat tgattttgtt cgctctggcg atacacttgt cgtttggcgc
     1021 ttagatcgtt taggtagaaa tatggaagat ttgatttcaa tcgtcaatcg actgaatgag
     1081 cgtggtgtga gttttcatag tattcaagaa aatattacga tggacaaatc aagttctaca
     1141 ggacagctca tgtttcattt gtttgcggca tttgcagaat ttgagcgtaa tttgatcctg
     1201 gaacgatccg cagcaggacg agcagctgct cgtgcaagag gacggtttgg tggtcgtcct
     1261 gaaaaattgt ctgaacaaga tttggaatta ctgaagactt tagtggatag cggtacacct
     1321 attaagacta ttgcggaacg gtggaacgtc tcaagaacca ccatttatcg ttatttggca
     1381 aaaatggagg agaaaaatgg aaagacaaat tctcattagt ttttgtcatt taatgctgaa
     1441 aatttttgcc atttatctag aaaagtgaca atagtatgag aattttttgt aagtgtctac
     1501 tgataactta ttatcaaatg ttgtaaggat tttattcatt tgttaatatt aaagaacgga
     1561 actccaaaaa agtccgtact ctaatttgca gtctctcact cttttagagt gagagttttt
     1621 attagatttt caaattaatt agtaaagtga tagaataaat tgatcataca aatcataaga
     1681 agaagcaggg tgatagcata cctatgcttc tttttttatg tatttagcta tttcttcttt
     1741 acaacatcga acatatgttt gtataatgtg gaagaatgga gtgttattta tgatggatta
     1801 ttcaaaagag ccagtaaatg attacttttt aatcgatatg aaaagctttt acgcaagtgt
     1861 cgaatgtata gaaagaaatt tagatccatt aacaacagaa cttgttgtta tgagtcgatc
     1921 tgataatact ggttcaggat tgatattagc ttcttctcct gaagcaaaaa agcggtatgg
     1981 tattacaaat gtgagtagac cacgtgactt accacaacca tttcctaaaa cactacacgt
     2041 tgttccacca cgtatgaacc tatatatcaa gcgaaatatg caggtaaata atattttcag
     2101 aagatatgtg gctgatgaag atctactgat ttactcgatc gatgaatcga ttttaaaagt
     2161 gacaaaatca ctgaatcttt ttacgactga agaaacacga agtcagcgaa ggaagaagct
     2221 cgctcaaatg atccaagaac gtattaaaga agagttaggt ttgattgcta cggtaggcgt
     2281 cggcgataat ccccttttgg ctaaattagc cttagacaat gaagcaaagc ataatgaagg
     2341 atttattgcg gaatggacgt acgaaaacgt tcctgagaaa gtatggaata ttcccgaaat
     2401 gacggacttt tggggaattg gttctcgaat gaaaaaaagg ctcaaccaaa tgggaatttt
     2461 gagcattaga gaccttgcta actggaatcc ttatacgatt aagaatagat taggtgtgat
     2521 tggattacag ctgtacttcc atgctaacgg aatcgatcga acagatatag caatcccgcc
     2581 agaaccgaca aaggaaaaat cttatggtaa ttcacaagtg ttaccacgag attatactag
     2641 aagaaatgaa atcgagctgg tggtaaaaga aatggcggaa caagtagcca ttcgtatcag
     2701 gcagcataat tgtaaaacag gatgtgttca tttaaacatt ggtacgtcga tcttagaaac
     2761 gagaccagga ttttcacacc agatgaaaat acctatcaca gataatacaa aggaattgca
     2821 gaattattgt cttttcttgt ttgataagta ttatgaagga caagaagtaa ggcacgttgg
     2881 aataacctat agcaagcttg tgtacaccga ttcgcttcag ctggatttat ttagtgatcc
     2941 acaaaagcaa attaacgaag aaaatttaga taaaattatt gacaagattc ggcaaaaata
     3001 tggttttacg tcgattgtac atgcttcttc aatgttagaa agcgctagaa gcattactag
     3061 aagcacactt gttggtggtc atgcgggcgg aaatggggga attaaaaatg attgattaca
     3121 atgatcgtgg acgtttaaag tgggcagggt tttatttatc tgatcatact gaagaaattg
     3181 atgccgacaa tgaacaacga accagtcaaa atttagcaaa agaacaaatg acgactgaag
     3241 agattgcgga agtattgaac gctgcagtca taaaaaatag atcagtatct attcaaaaag
     3301 aagaacgaaa cgctgaagga tactatccag aagatattgt tggcaagatc ttaggtagtg
     3361 atgagctggg gatctacgtt ggaaaagaca aaattcacta cgacgaaata cgtcatatcg
     3421 aattttacca ggataataaa tggagtcgat tagaatgaac attatccagc aatatgagct
     3481 aaagtatata acctttgatc agctatcaga agagatttgg ggttatggcc aacgtttgat
     3541 aaatgaggtt ggtgttgagc gcttctcttt ctatgtagaa gctgcagcag gttatcataa
     3601 ttttaggttt tatattttcc cgttatatat ataaggtatt ttacggattc tatagagtta
     3661 tctctatgaa tccttttttg tttttacaaa taggagtaga tactagaata atgcgtgtgt
     3721 taaactatta ttgcaaacgc aataataaaa gaaagtaggc ttacgcacca tgtctatcaa
     3781 tacatttgtt tactcgcacc cgatcaatgt ttacattatc aagaatttag gaatcactgt
     3841 agaacaattt tgtgagttat atgcctaccc acaaggaacg gttgcaagct ggataacccg
     3901 acaacgtaga ataaaatcat tacctgcttc attcgtttat gatttaagcc tggcctctag
     3961 tttaaacatg tctgatgttt atgaaaagct cctgtcttta gaaaaagaat atgattcctt
     4021 caagattaaa catgataaaa aaataaaaaa acatatataa tatcaatcac aattgtataa
     4081 tattacgata tcttaatatc atgatatcgt aatattgttt ttttctaaga aaatgcacta
     4141 taagaaggga attattagta acaatataga cagtttaata atgtttaaat tattctagtt
     4201 gctgctcgca aaacatattt cttatgatta ataaaaaatt ttaaagtaat aaaaagtgat
     4261 tattttactt tcgaataaca tatgaataat ctttatatcc gaaccgatat atatctttaa
     4321 aacaaaataa tcacacaata ttacaatata atgcatatac gatattgcaa tatcacaata
     4381 ttatgatata attatattat aatataagat gaatggggga tattttaatg gcaagaatat
     4441 tgtcagtagc caatcaaaaa ggtggagttt ctaaaactac gactagcgtt aatttagcag
     4501 catgtttgaa atttgtttac ggtaaaaatg tacttttagt ggacattgat cctcagggaa
     4561 atgctacaga taatgtaggt ttcgatatag atggaacaaa ccaacctaca atttatgaag
     4621 tattaaaagg ggaggctgac attacagatg ctattctgga ctataaagga attgatgttt
     4681 tgccagccga catagccctt tcttctgcag agagggaatt tactcaagta ggaagtgaac
     4741 acaggcttaa aagagtactt caaccaatca aggacaacta tgattatatt ataattgatt
     4801 gtccaccaag tttaggaatg cttacagtca acgcatttac tgtttctgat gaaattatca
     4861 ttccagttga agctgcgtat ttctcattaa aaggactaat aaagttaagt gaaactattg
     4921 aaacggtaaa agaatatacg aacgacaagc ttgaagtacg aggagtccta tttactaagt
     4981 ataataagcg ttataacatt tctaaagaaa tgacgactgc agcaaccgaa atttctagag
     5041 tcattggtgc aaaaattttc aaaacttata ttcgtagaac aataaccgtt gacgaagcgc
     5101 aagctgctgg atcagatcta atcaatttta ataaaccctc aacagctgaa gatgactata
     5161 aagcatttac agaagaatat ttaaaagaag ttggtgattt tgatggcaga agataatcgc
     5221 agtcaagctg cagtaagtag actactagga aataaaaata aaaagaacta caaaccaaaa
     5281 gaagtagaag aaatcgaatt aaccaagaaa aatataagta aagtcataaa agatcaagga
     5341 agtagtgtaa gaacagcaga tagcattcgt ataaatccaa ttctaatgtc agcactcaaa
     5401 tattggacga ctattgctga atcagacaaa agtaaaccag acgttataga agatgcgcta
     5461 ttaaaatcca tacctgaaga atacttgatt gaaggatata aactagcaaa gaaacaaaat
     5521 aaaatttaat ttacattcaa tatcacgata tcataatatc gtgatatttt ttttataaaa
     5581 atcagtagta tcaaagctga taagtcgctt atcttcatat tgtaatatta cgatatcgtg
     5641 atataatgtt attgtaatat cgtgatatag ttaccatgag cttaaaccct gaaatagagc
     5701 ggtttgcttt aatttatttt ttaaggtaaa ctaaaaaaag ataaaaaaag actactgctt
     5761 taacaaagtt tggcgactta tcagcagtag aaaaatatat agtggataga tatatttgtt
     5821 taattcattg taacatagtt ttgaacaaaa cgctatccta cagaaagagg attggctatg
     5881 aatgatttta attattacaa atcaaaagaa atttatcgtg aaaaatatta tcaaatgcct
     5941 aaggtatttt ttactaacga aaaatacatg gatctttcaa atgatgcaaa aattgcttat
     6001 atgctactta aagatcgatt tgattattca gtcaaaaata actgggtaga ttctgatgat
     6061 aatatattct tcatattcac agttgaagaa ttaatgaaat tgttacaatg ccgcgaaggt
     6121 aaagtatcta agatcaaaaa agaattagaa aaagcaggtc ttctgaaaca gaagagagga
     6181 agagtaaata aacgtgatgg taagattgaa tctatgccta atttattgta cttaggcaag
     6241 ccagaagtta cgaatcaaga cgtctttaaa atcatggaag aggaagacaa tactgattcc
     6301 accgtaattg cgaaaatcgc aaatacggca aaaccgcgaa aaatcaatga ttctaccgta
     6361 attgcgaaaa tcgcaaatac ggcaaaatcc agtcatatca aggactcaac cgtaattgcg
     6421 aaaatcgcag acaatctatt ctattctaat tctatagaca ctaatagaca cataatagac
     6481 actgaaactg accagttaca aaatcaagtt ttgctagata acttcgtcga catcatgcaa
     6541 gaggacagta taaatacatt tgtaccagaa aatgtattaa acttgataaa aacattttct
     6601 tcaagttaca gtgaagctca acaaaccgtc caaactattc ataatgcaaa gaaaaaagct
     6661 gaagaattag agcaaactat tgtcgtttat gaagaactgg aatcttatgg aattgatgct
     6721 gacaaaggat tatatatgac tttactgaaa gcttatcaaa aacaaaaaac tgaaaaagta
     6781 aacaatttgc agaatctaat tttcgtttat gtaaaaaatt ggtttgtcga aaaagctatt
     6841 gcggcaaagc ttgcaaacga acaaagagaa acatttaacg aattaccaac agttagtacg
     6901 gaaaattggt tagagtagtt gataaaaagc aattactata aaaatgatca attttaaatt
     6961 agaacttttt tatgatttta attttattac aaatattaca aataaatagt tagtattccg
     7021 gagtttttag attaaaagtt tatgaaaaca atcacaatcg taggttttaa attcataatc
     7081 gttagatatt ttagttattt ttcactcaaa aaaataggga actagtgttt gcatatgtgc
     7141 ttttttgagt tataataatt catgaagaga ggtaattcac ttatttcccc aagctgaaaa
     7201 gcctgatatt acttcttttc aggcagtgct gctaatattc aattacactt ttttgtatat
     7261 ttttagttgt tttaaattta attgtctgct atgcttgtga tgaattgatt ttcgttctgc
     7321 acttttggac gatgaatttg gacaattgaa aatacaaaaa aaagaaaggc cctgtgagac
     7381 tcggaaggct ggtaaccttc gtcgacgtcc acaggtggca ttaagatcac tcccttaatg
     7441 cgtttacctt tcagtgttac ggtattaccc gtaacggatt tagtataaca gatattcaaa
     7501 agtgatacca ctttttttgg ctgtgttaag gttaaaaata cgtgatatta cgatgaatct
     7561 atgcctaagc ataatgttat aaaaatgtcc gtgtggctct ttgccgtatg acgaacgctg
     7621 aaattagagg gaacgcatgt ttggatgcgt tccctctttt ttgtttgtca tgaacgaaaa
     7681 tcaattctct atttttgaaa aataacaaga aaaacttgtt aaaaatattt aaagtaagtg
     7741 ggtggattat tttatgtcat atacaggaat tttgagtctc gaagatattt gtcactacgg
     7801 taaacggtgt accgctacag aaaagatcac gaagaaacta tcaactggtc aaagtaaaac
     7861 cgttgtgcag tgtaagaaat atataatcca gaaagacaaa gtctcagaag aaatgattta
     7921 ttacattggg aaacaaaaac aaataatttt aaaagatcct atccctttaa aagagctata
     7981 tccaacaatc aaacatgttt atgaccaaaa cggtgtactg attggtcgaa gaaaaaatgg
     8041 agtcttgcgt tgtaccgcaa aagggatggg gcgtttaatc agctgaattg atcattctca
     8101 taacttgtat tttatcgttt ttcgcttgct tgaaaatatt gtcgagactt ctaaaggaac
     8161 ttagaatcac tagaaaaata aggagtagac ttgatctatc aacaattcct ttcggggggt
     8221 gacggggtag gtttttacga ttggtaacgt aaaaaattgg tcaatcgagt aaatgccctt
     8281 gtttggatag tggtgaaatg aatttgtgta aataatatgc acagagtaaa caagagggaa
     8341 tttagaagtt agacaagtta gaatagcgcc tagattaatc atgagcagct actaagggat
     8401 ctaggctttg acgcaaactc atggaataag gagtaggtct tctcgtaatg gggaggacgc
     8461 acaagaaatt gtgtcgtgtg ctgcagtaca tgtagatact tgcgaagacg ttgatctttg
     8521 gatcaatcga gcatttaccg ttttctacta gggtttctcg tttcggacgc cataaacgag
     8581 tcgatttatt ttatatattt ttgagtgaac gaaaaacgat aaaaatattc gttatgaagt
     8641 aaaactaatt tagtagatag caaaggtggt agaggaatga ttcaaaaatt tgcgaaaatg
     8701 agtaaaactg catggattga aaagcttgaa aatgacttga gtgtcatgcg attagctcca
     8761 aagaaattca aacgagagat tgcagaaaaa gaagcaattt tgtcggtatt gaatgcggta
     8821 gacgataaat gtccagtaaa aattagtttt tcagatatag caacaattat cgaaggtatt
     8881 tattatggtg aagttgatga agagatatct tctacaaaag atttattaga ttttttggat
     8941 aaaaacaaag atacctttat agtactaact gattatgagg aggttacaac aaatgaaaaa
     9001 ttatgatccg aatagccgat ggggaacaca tacgattaag gtttctttcc aacgttggga
     9061 ttacaaagga tttgtgacgt ttagaagagg cggaaattgt aaaggattag atgttttagc
     9121 attagacgag gatgatttat acgatcaaaa attgactgat aatccaattg gattcggctt
     9181 gctacctgaa gatgatcaag gtaatgaatg gtttaaaatg acgcttatga atgataatgg
     9241 agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg tcagcgttga
     9301 aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa ttttacagat
     9361 aaaggcttag aaggagtcac gacgattaat aaaaaggtag tattacctta ggcattgagt
     9421 aaggaattta tttaggagga aaattagatg gcgcgaccag ttttagaggc gatggatatg
     9481 gatactttga caaaaggaag gtatgtacag ctgttagctc gtggttattc acctaaagaa
     9541 gtattcaaat ccctgtctaa aggaacagat atggaaaaag aaagattacg aaaagaattc
     9601 gattattgga gaacccataa tggaatcaag gatttgaaac cagcacggcc agtaattaaa
     9661 cgaaagaaaa agataaccca agactgaagt gtagagcgta tgtgatcata ggctttttta
     9721 tttttgcaaa aaacaaggaa ggtaggaaaa ctcatgaatt atgaagaaat cgaaaacaga
     9781 aaaaaagtgt cgaaagaaat ggaagaaaag ttgctaaaaa cgatgaaaca aaaacacctt
     9841 aaaagattat ctgtcatgca atatatcaat gatatgcaga taacaggaaa agagaaagct
     9901 tgtttgttag gttcaatgaa aaactttgaa cagttgagac gaacttatgt gaaaaaaagt
     9961 tctaattgtc aactattgtt agaagtctca tgacattcat taatagaatt catttttaaa
    10021 actttttcta tggaaatgag aaaaagattt tttgttttgt ctgaaagggg gtagctggat
    10081 gagaaaatgg ttatttggtt ttttaggagt cgctttaatc gtcttttgca gtgtgtttgg
    10141 ctatgttagc taccaaaaac atgaaggaaa ggtgtttaag caaaatatag aaaaaaagat
    10201 gcccgtagat caaatcaatg aacatgcaaa gagttataag gaggatgcaa cgaacgtcaa
    10261 taatgatatg tctttggggc aaatgttgtc catacaaaaa gaagcaatcg aaatgggtgt
    10321 caataaacaa gcctttgcac aaattcagat acctgcactt ggtttagcat tgccaatttt
    10381 taaaggggca aatcaataca ctttgagtct tggagcggct acttattttt atgaagatgc
    10441 cgaaatgggc aaaggaaact atgtcctagc aggtcataac atggaaatgc caggtgtttt
    10501 attttctgat attcaaaagt tgtcattagg ggaggtgatg gacttagtta gtaacgatgg
    10561 cgtctatcgt tacaaggtaa cgcgtaaatt tatcgtacct gagtacttta aattaatcga
    10621 tggcgttcct gaagagaact ctttcttgtc tttaccaaag aaaggagaga aacccttatt
    10681 aacgttgttt acttgtgttt atacgtcaca aggaaaagaa agatacgtgg tacagggaga
    10741 gctacaataa caaggtttta atcaacgcga gtcgttgaaa tatgaaatga ggagagtaaa
    10801 aaatggcaat aaatcggtat gtaaaaaaag ctggaatgct tgttggaatg ttaggaatca
    10861 ttgcaagctg ctttcagggg tatcaagtac acgcagaaga tgtcacgcaa aaaacaccac
    10921 cagagaaggt caatatcacg gttcataaat taatgtacga tcaaggaacg caattgaatg
    10981 ttgatattga cgggatcaaa aacgatggtt atacgcacga tcaatatcct acaggtgtaa
    11041 cgaaatacaa caaggcagat tatggtgatg ttgagtttac gttaggaaat attacggatc
    11101 aagtattacc gagagaagat tctgatctaa ccaacgcgaa agtggacgag attgtacaag
    11161 atgtagaaga caagggatct aacagtgagt acgtcaagaa tgcaaccaac aaaattactt
    11221 cagctgtaga tgaaaatggt gagatcactt ttactgatca accagcctat gtcaattcca
    11281 aagggaatgt atatgtggtt tatgaaagta aatccgctgc tggattagtc acacaaaaag
    11341 caaaaccaat ggttgtgatt gcgccaatga cagataaaac aggatcagga tttttaaaaa
    11401 atattcattt gtatcctaag aacattgttt ctaagttgag ttttgaatta acgaaatttg
    11461 gtgacgatgg cgcagcccaa agcaaacaaa cccttttaaa aggggcaaaa tttgagttat
    11521 acaagggcga acctggaaaa ggaacaaaat tgggtgatct tgtatcggat gatcaaggaa
    11581 agctgactgc gactgatttg actttaggaa aatactattt tgtggaagtt ccttcggaag
    11641 ttgtagtggg atcagatcaa gaaccaacag cagatcaata tttattaggc gcagacgctc
    11701 gcaacgatgc gcataataaa ttaacctttg aaattaccaa tgacggcgtg acaagtgact
    11761 taaaagcttc ctacgtgaac tataaagcac cagtgatcga taaaacagtg acaaatggaa
    11821 caggacaaga acattctttc caaattggtg acgcagtgaa ttatcaagga acgattcata
    11881 tccctacaga tattgcaggt ggaacagatg ggattacagt aaatggtgtg aagagtgaaa
    11941 cttctcctta cagcgtattt aagtggggag atacagcagg acaaggactt tcttatgtcg
    12001 ctgctaaagc aaatatcaaa gtaacaaata aagatggatc tgtggtatta aaagaaaata
    12061 cagattataa aatccaaaat tcagaaaatg gctttgtgat cgattttatc gtgaataatg
    12121 gccaagtaag tgatactgta gcaagtttac acggacaaga tcttcaaatg acttataaca
    12181 tgtatgtaaa tgacagcgca gctgttgcca atccattaac aaatagcgtg gatttcgttt
    12241 ataacaacaa tccatttaat caggaagaac accacgaaaa aacgaaagct gacgttgtga
    12301 cttatggtgc aaaattctta aaagtcgata gcggactatt tggaacagga atcaaagcaa
    12361 caccgcttga aggcgcagaa tttgctgcta aaaacgcaga aggaaaatat tatggtggat
    12421 tagttgatac ggataaagac ggtgtcaaag aagcagtatg ggtagacgat gtagcaaatg
    12481 cagcgatcct aaaatcagat aaagaaggtc actttgaaat cactggttta actgaaggtg
    12541 aatattcact ggaagaaaca aaagcaccaa ctggttacca aaaattaaca gaagatattg
    12601 catttaaagt aaacaaaaac tcatttaaag aggaaaatcg aatcactgtt aaaaataatc
    12661 aaaaagcagc tattccatta acaggtagta atggtttcca aacgtatgta ttgatctctt
    12721 gcttgttgct aggagcaggg gcactttctg cagttgttta ctttaagaag aaagcctaga
    12781 aggatcagct aatgattatt cgtaagcccc caaaaagaag agtcatgcac aaactgatga
    12841 tatttttttg gttgctgtcg gcagtagcag tctatttaat tccagctgtg gaaacaaatg
    12901 tagcgaaatt tcaacaacag caaagaattt tagccattga aaatcttgaa aaaacagatt
    12961 cttctttgga tgttgcgaaa atagagttag gcgatcctgt tggtgttctt acaattccaa
    13021 gtattccttt aaaactacca atttatgacg gaacttccga caaaatttta gagaatggtg
    13081 tagggattac ggaaggaact ggcgatatta caggaggaaa cggtaaaaat cctttaatcg
    13141 cggggcacag tggactttat aaagataatc ttttcgatga tttgccttca gtaaaaaaag
    13201 ggcaaaaatt ctttattaag gtaaatgaag aacaacatgc ctatcaaatt tatcgaattg
    13261 aagaagttaa agcagaagaa ttacaaaaaa attatttaga ttatctcatg cctgaaaaag
    13321 accaagatag gatcacgtta atgacatgca cgccaaaagg tgttaactca caccgctttt
    13381 tagtgtatgg tgaacgagtg tcattcagta aagaagattt aaaactacag aaaaaagcaa
    13441 aaaaatagga aatcaatttc attggattgg cgattagcac tgctattcgc tttttttgcg
    13501 ttcctattat ttttatttat aaaaaaataa ggaggagatc ggaatgaata agagaaaatg
    13561 gctgtttgtt tgtgtagcag ctctctttgc aactgcagct tactcacaga tatcttttgc
    13621 tgatagcagt actactccaa ctaccagtca gacaacagat agttcaacaa ctgcaacaag
    13681 tgacacggac agttcgaaga acactgcaga agaaacggca gattcaagta cgagttctac
    13741 tgaatcagcg gaatcaacgt cagaaagttc ttcagagact tcagaaaaac tgcaagaagt
    13801 agaagatacg caagtaatca agcaaaacat tcatattcaa aaaattattt cttcgaatcg
    13861 aatcaacaat caaggaaggc aagtgactca gcaagaaggt gtcaatggtg caaagtttat
    13921 tgtctacgat atcacgaata tcttagaaga aatgacacaa gatgataatg agaaaaatca
    13981 atcggtagaa ataattgagt caaaattgaa agatcgagca aaaaaacttt catacgatca
    14041 gttgaaaaaa gtgactgaag gagaaaccca aacaattgat gatcaggaag gcgtgattga
    14101 tttttcaatt gaagtacctg caaacaaaaa acaagcatac tatatcgtca atgaatcaag
    14161 ccctgaaaac atttcaaata gcgaagatat tgttttattg acacctgttt cagatgaaaa
    14221 gggtgaattt ttgacggatg tttggttata tcctaaatca aagaaatcag agccgaaaga
    14281 agaaattaaa aaagttgtat caactggtgt aaagaaaaac ttttttgata attgttggga
    14341 ttttttcact catttatttt ttaacgatta ggatgaaatg atgaaaataa taaaaatgtt
    14401 agtttttaat ctatcttttt tagccgcaac aatattgtgt tgcggtttag ttttttctca
    14461 attatcaatt tacccaagat atcaacgatt gttcgttcat tttctaaata tccagcatgc
    14521 ttccatgcca ctagaagtga ttaacaagct cttaatcaat ccaattttta tggctagttt
    14581 gtttcttctt gcaggattgg taacgtgggt attaggaaaa aaagaatgag aagcttacaa
    14641 tcgtttaaca aaataataga tggagggcat tatggaaaat aaaattcatt gcactacggg
    14701 aagagtattt actttagtgt tgtttttgtt ccttcttctt tttgcaaggg gaactgcagt
    14761 ccaggcctta gaaaaagttg atccgagtca aatcaaagaa aataataatc tggtacttta
    14821 tcatgatagt gattcattga gaaatatcga agtaactggg atcaatggat ttaagatact
    14881 agatgagtat tcaactaaaa aacaaaaagt acttgcttgg ccaaaagtca aagatttgaa
    14941 aagcacgtca aaaattgttg cccattatca aaaggtgggc acttatctag gaaaagaaat
    15001 tgattgcgac gtaacttttt ctaattttgt agcgcacgct ggggttaacc aagacggatt
    15061 agctaaaaca attaatggga aaaagtatta taattatttg ctctttgata aaaacattta
    15121 tcgaggcttt ttcatgcagt caatcaacgt tatgaatgtt aaactagatt ttaagtatgc
    15181 aggatcaaaa acaaaagtag ctttgagtga accacagcca gaaattaatt caaaaatgag
    15241 atcgatgaga gcagctggta ttgagttgaa tgatacattt ctatctattt acagtttaaa
    15301 tggacagtat cagcaacgtg tgtcttggta taatgacaat aatgaatctg tcggtgaacg
    15361 taatattgat atgagagaat ttgttgggta cgaaaaaatg gatcgcttac cttattttgt
    15421 cacaacagat acagcatgtg cagaatacaa agctcctgcg ttatcaacaa acaatttaac
    15481 ttcaaaagta gtgggaggac gtgcagaaaa ggcttatagc tcgaatgatc atttcaccga
    15541 tgttgtagga gctgatactt atcacagaag tggtgtaacg tatacgcttc aaggcgcttc
    15601 cccaacattc atgattggcg caaatacgaa tagtatgatg tttagctttg atactgcatt
    15661 gctttggaca ccacaaccat cgaagcctac aaaagaagtg tttaacaaag ctaatactga
    15721 agaggcagca cacaatattg acaaaaaagt gattccacaa ggatcagatg tttactatca
    15781 tattcaccaa aagtttgatg cattaacagt caacacaatg aacaaataca aatcatttaa
    15841 aatcactgat acctttgaca gcaaaaattt tgatatggta tcggatggga aaaactatga
    15901 tggcgcattg tggtatgttg aagcgaatgg gacttggaaa aaagctgata caggaggaaa
    15961 attaactgta tcaggtaata cagtcactta tactgcttct gacagctttt tagctaatgc
    16021 tgcaaattac ggtagggact acattttgca gctccatatg agagcaaaaa atgatgcaaa
    16081 aggaattgca ccaaatgtgg ctactgtaac gtttaacaat gatccgaaca aaggaacaca
    16141 aaaaacaaat aaagtagaaa actatttctc tcttaaacca aataaaaaag tggaacaaga
    16201 tgggaaagat gttgatggtc gaaatgatgg taaaaaagga acgcctacag ctcctttaaa
    16261 tgcaggatct gaagtacaat atctagtcac acaaaaatgg catacaaaag gtgtagacat
    16321 tgcgctcgat cattacaagc aatttagtat tcaagatcca gtggaagctc gtttgacgta
    16381 taaagaaggt tctgcgcgag tgatagatat gagtacagga aaagatatta cttcagaagg
    16441 tatactaacc tatgatagcc attcaagaac gctgaaatgg gaagcttccg ctgatttctt
    16501 aaataacaat cttttagatg gacgagaaat tcaactgata tttacagcta aaactccttt
    16561 gaatgaagaa ttggatatcg acaaccaagc aaaagtttca gtagaaagta ttgagaaagc
    16621 aactaatgta gtaacgattg gggtaaaacc taacttacct caggtcattg tgccaaaaac
    16681 tggatcgaat catttagtgc taactgttat tggaagtata ttatttctta ctttagcatc
    16741 attgggctat gtagttttaa aactcaaata aatataaaaa caaggctgat tttcagcctt
    16801 gttttctttt atttgaagat tggattcgca ctaataaata cattatctac gccaattcta
    16861 ccagcagagt caactacaaa attatgacct aatccgagaa tactttggtt attataaagt
    16921 aaatttgcat tatgggcagg gctatttttc catcctacca tggcatcgat agcaccatcg
    16981 tctgataaaa ggtcatcaat atacagccca gtttgactta tgttttctcc cttagcacca
    17041 tagcagccta agctttccaa taaatcttgg aaagtaccac caccagggcg atagtgactg
    17101 aagctgactt gtaattcttt agctcgaata tctgcagcct gttgaacaat atcattgaat
    17161 tgcattgggg caacaccatt ttgttggcga taatcattta accaagcaag ttctttttga
    17221 cgcataaagt tacgtaattc gtctaatgaa atctttttat atttgaatgt gattgtttta
    17281 ttttgatcga cgttattaat ggatcgttgg ccgtttttat ccacggtaac ggtaggatcg
    17341 tcatttctgc acaagaaata attttgatca gtcatatttt tccaaggagt agagaagcta
    17401 tccccttcgt atactgtttt tgattcacta gagagaacag cgttggtatc agcatttaca
    17461 tgattaacag tcacattaaa tttacgagtg tagttgaaaa tataattttg actttcagtc
    17521 acattgttta aggttactga atttgttggt tctacccgat cgtctaaaac atatccttga
    17581 atagaaggag cagttaaagt gacactttca ccgtgagtga cgttgattat ctgttctttc
    17641 aaaatatcaa ctcgcccctc atttttgtag ttgttattgc acaacaaatt gatttgatgc
    17701 acttgattat aattgaatgt gattgttgta ttacctgtga ttgttttgct ttgagtatca
    17761 ttaccaatca ctttgtagct aaaattatct ttagcacctt cgctatattt aaatgctttt
    17821 gccttagcag tgtaagtaga accagaagtc acttttgctt gatctgtagc aatcacttga
    17881 cctgtttgat tgtcaatatg gttaatggta accatatatt cttttggcgt ttcaggtttg
    17941 tgttgcaacc ctttaaatgc agcatcaaga ctatttttag cattgtttac ttgtgtttga
    18001 gaaaccttag aatcgtctag tactttttta gcattggata aagcagtctg aaaattattc
    18061 caagattctt ctgtaaaatc tcctttagca gttgatttta ctcggttgta taaagcttgt
    18121 aattcagctt tattcacagt aggaactggt tttttcttta atcctttgta agcagaatct
    18181 aatttatttt tggcactatc cacttgcttt tgagtcgctt ttgagtcatt taacactttc
    18241 ttagcattgt ttaaagcggt ttggaatgcg ttccaggtat tgtcggtata gtcacctttt
    18301 ttcgttccct taacgctcgt ataaagagct tctagagccg atttattcac tttgggttta
    18361 gaggtgtcag cgccatacca accgatacct tcgtatttcc agccatagtc tttggttaac
    18421 cattgtactt ctttccaact tgcagtgtag tgatgtgagc cagattttac accaggaagg
    18481 aaaaggcggt agatcggttt attatttttt tcggcagaac gccaggcagg cccttcataa
    18541 gtccagccgt aatttttagt caaccatttc acttcatccc aactagctgt gtaatggtga
    18601 tcacgaagac ctttattgta caaacgatag acaagatggc caccgctagt cggtgcttcc
    18661 caggcggggc cttcataatt tccccatttt atctttacta gatggtcacg ttcttttttg
    18721 ctagaggtgt aaaaatgttc attattattt ggattgtaaa ggcgatacat gacgttagtt
    18781 gaagcagcgt caacagatcc agcaagacta agtgaggcag caaatgttcc aatagctaaa
    18841 acagtttttg gattaaataa tttcattttt tcaactccta aatttaatta aaaatgttta
    18901 catgattatt atatatcggt taaccgatat agtcaataaa aaataagaaa agccttatat
    18961 aattttggct tttcttattt tatttgttag ttgaagtatt aatatcaacg atttcttcag
    19021 ccaatttatt taatttcata gcatttatga atctcatgtt gtctatacta gagcgtttgt
    19081 tggcaaggtc acttactgta gttaagggta cgccagattc tatggatata gaacgaactg
    19141 atttaccact atcaaacagc aaccattcga ttttcttttt atcagcgtac atttcaatgc
    19201 tccttcttgt tttttcttta ttatagctct tttttgtaat gaatcacaat atcaagagaa
    19261 aaattttcta tagaaaggtg gatttttatg agtattcgtt ctccgtgtat ccaaatgagg
    19321 tttgttgcta ctttcgttat ttgatatact aaaaataaac aacgaaagaa ggaataaaat
    19381 gaagaaaaag caactatggt ttgtctctat gtttgcaata ttttttatta ttttaagtgg
    19441 atgttcaagt aataagaaat cggaagctaa agaagataat agtttattgc aaccaggaac
    19501 tgtttggaaa gaagaagtag gtggtcttat tcataattta aaaattatag atgatactac
    19561 ctgggaatat tctgaaagtg tatggcatcc agatcctgta caaataacag ttaagaggca
    19621 aaaagactat gaaggtttag aaagatacaa aatagtagat tctgcaggca ttagggaatt
    19681 tataaacaaa cctgattcat tatttattgt tgttccctat gagaaggatg gcgtaaaaaa
    19741 aataattttt cttggatcta gcaaggatga gaagcgaacg aaagagaaat taattcatga
    19801 tggatcacaa tcaaataaat acaaattaca aaagactagc gagtgattgc tagtcttttt
    19861 atttgtcctg aaggaggaat cacagtggca aaagctaaaa cgccatttga aaaagccgcg
    19921 tctaaaatct tagaacgccg cggtgtatct gaaacacagt ggaaacaaga agtccttgaa
    19981 aaagcacaat ttgacctatt acggggaaaa gacaaggact tagaaggata tatctatcaa
    20041 cgtgaacgtg aaaagcttgt tttcgatgaa attattaaac actaaaagga gcgaacaatt
    20101 ttatgtttga atcaattaca atgaattttt taacacaacc gaaagtagta gctgcggttg
    20161 atatttttag agcattacaa actgccgcaa ctagtttgaa aaataatctg atgatttttg
    20221 ctgctgttgc ggcagtggct tccattgctc ttggaggtat tttcttcttc tttgggcgaa
    20281 gaggagctga aaacgggaag acacacgtag gttatgtatt gttgggggta ggagttattt
    20341 tagctgccgc ttctattgta gtttcaatgt tctcaatgtt tggacaaacc ccacaaggac
    20401 tataaaaaaa caagtagaag gagtaaaaag tacgtggcta ctttttactc ctttttttat
    20461 ggaaggagaa tattcatgtt ttttacgatt tcaggcaatg caaaattgga taaagagtta
    20521 ataagaaaag caacgaagaa agtgggaaag cgtacttcct ttttaccgaa aacaattaaa
    20581 tattctgtca ttgaaacgga agaacagcta catgtgttag gacaagtgtt agaacaagag
    20641 aatcaatctt ctgttaaatt gactctcacg ttacatgata aagaaggaaa aatagaaaag
    20701 gctggcacag aatacgtttt tggctcactg gattatcaaa tttcagaagg tttttatcct
    20761 acattgattg atttaatccg cctagatata tttaaagata cggatgaaac catgagtgtg
    20821 gaagagtctt atcgaaacga aaaaatttta caagttttag aagaagagtt agaaagcgac
    20881 caaagaaaag tgaatcaagt gagtgaagtc gaagcaaacg aagaagtacc atgggaacgc
    20941 ccaatctttc caaaggatga atcaaaagaa aaagagaaag gaattattga cgaacaagct
    21001 gacattttga ctcataaatc aatatttgag gaagagaacg aggtaatggg tcaattggag
    21061 gtagatccct ttgaagaaac aatggaggat gaagagacgg ccgaagtaga agttgatcca
    21121 gttgaagcta atgaagaccc aacatggact gaaggggatt ctcaggaata tattgaacca
    21181 cttatcagtg aagaagtaga gaaaaatgaa acagagatac aaaaggcaac ggtatttgat
    21241 tatattttgg atcaatatcc gccagagcat gaatggatga aagaaaaaaa taaattattt
    21301 ttaaatgaac tttattcgaa atatcaatta cctcaaacct atcaagcatt tttagagagt
    21361 aaagaagaat taatctcaca gggtaaaggc acacttttag aacagttaga gtctgtccaa
    21421 tcaacaaatt ggcatgagct tgcacaagaa gatttattag ctacttttga agaacgtcaa
    21481 caagaaacag atcaagaaat taatgagtat gcgacggaac agtcgtcgaa ttgggagaaa
    21541 gcaaaagagg aactaaataa agaagaacag cgagtgattg aagaagaagt acaaaaaatt
    21601 aaagagcggt acgaaaagaa acgtgacaat gcgtattcct tgtgtgtaga aaagatcaac
    21661 aattttactc agacgaatca agaagcatta tcgaaagaaa aagaaaactt attaaaagaa
    21721 cgtataaaag aacgaaaaga agaattttac cgagggttgc gtactgataa gctaaggatt
    21781 agcaaagaat taaatgaaca actgaataaa ctctatcaaa ctacgactga agttctgatg
    21841 gaaaaacacg agcagatcca acaagaatta caagcacaaa ttccaaaatg gaaaaaagag
    21901 tatgaaattc accaacaaga attgaaagaa caagaagaaa aacaacggaa aaaagaaaaa
    21961 gagcgcgaac aattagaact aaaaaaacgt gaacaggatc ttgcggaaca aaaaatggcg
    22021 atagaaaaac aacggctaga acaagaaaag gaaaaagaag cagctgctcg taaggaacgt
    22081 caagaacaaa tggatacatt gcgacaagca cagttaaatt atctgacaca tcctcaacca
    22141 gtagctgctg ttcaaacgcc agtgaacaac caagcaaatt taactccttc accccagcgt
    22201 tcctttaacg gatggatggt gggatgtatt gcaagtttaa gcttgcttct aggtggagga
    22261 acagtatttg cttttaatca tatcaatcaa tcttcacagg ctgattctgt agccacaacg
    22321 gcgtcaatca aggaagaagc ccaggaacaa gcaagaaaag agtatgaaca acaattagaa
    22381 gaattattga atcgtaccaa tcagtcaaca acagagtcaa gcatggaaaa tagcaaagag
    22441 agtgattcta gtaaggctaa agaaggatca agcgattcaa ctgtacaatc aaacacttcc
    22501 gaaagttcag aatcaaatga ggtgaaataa tgcgtgttag agaaacaacg aagcgaaaag
    22561 acaaacgatt caatttagaa aagaataggt atctttctca tttctatcgc tatactccct
    22621 caggagaaga gctaccagaa aaacatgaca aacgcatttc gaaaaagatt ttttatagtg
    22681 gacttgtata cctcccttta gagtatttag taacaagagt accagaagtt ttcaaccgat
    22741 tggaaattgt taagggaagc ttacaaggaa taaatctgtt tcaattacag gaaatgccac
    22801 aggtctttca ttttgattca gctggttttt ggaatccatt tactcctaca actagtttgg
    22861 gtacacgttt agcaatttcg acagtttgtg tggttccagc tgtacttatg ggattgaaaa
    22921 tggacatgtg gtttcgtcct ctagcagatg gacaaaaagg ggataatcga ttaatgacct
    22981 taaaagaaat ccaacagatt tatccgcaaa tacctgaaac aaaaactact tttccaggtt
    23041 atggaggtat tccaatcaca cattacaaaa aatattggta cattcaaaca gatactgtta
    23101 atacgtgtat tgttggtacc tctcgatcag ggaaaggtca gattgaagtg ttagccacca
    23161 ttgacaattt atctcgtgca gaaaaacaaa gttcaatggt cgttaatgat ccaaaaacag
    23221 aattatatgt tggtagtaaa gatgaactag aagctagagg gtatgatgta tatgccttta
    23281 atatcaccga tccactgcaa tcaatgagtg ataatccatt ggcactcatc gtgaaatact
    23341 ggaaacgagg agacatagat actgcaacac agttaaccaa tactttcaca aatacgattt
    23401 attatgatgc caacgcttca gataacaaat actttaacga aaacgcgcag aaagctgtca
    23461 atgcgcttat ttttgcgttg ttagaacaag ctgatcaaac aggagattac tccaaattga
    23521 caccgaataa tctcgttgag cttctttctg agattggcgg ttttaattat tatccagatc
    23581 cagacaatga attcaaacag ttgaatgcct tagatgaatt tatgaatcag ttgccacctg
    23641 gaaacattgc aaaaaaacaa tatggggcaa cgaaaattgg atctgataaa gcgaagggaa
    23701 atattttatc aacggcaatc acgggattga atccatttac tctaacgaaa attgcaaaga
    23761 tgacttctca atcaacgatt gattataaat cggttgggtt tccaaaatac ttagatttga
    23821 aattacatga aagtctttta aatcaacgag tcgtagtaga attttatcat aagggaaaga
    23881 aatttcatga agaacgtgtc aaagtaggct ataaaggatt tagtgaaatc aattttgatt
    23941 gtcatttagt tgcaggtgat caagtttgtt taaaatacga tcatcaagga aaagaatatg
    24001 tggcttggta tgaattttct caaagagtat tgaaagacga agagggtaat atcgtttatc
    24061 ggaaaaaacg aggagaaacc catttgccag aattagaaaa agaagccgtt ctaacattag
    24121 atgaggaaaa aagtaactta ttagtagagt ctattcggat gcactatacc gataaaccaa
    24181 tggctatttt tatgcttaca cctgattatg atccatctaa ccatatcatt gttagtattt
    24241 ttcttagtca gttatataaa gaattatcta cgcaatgtgt taagactaaa ggagataaat
    24301 gtcaccgcag gattcatttt attttagatg aatttgggaa tagtgtgacc tgtttagcct
    24361 aaggataaaa gaggctagtt ccaacgaaca ttggaataac tttactatcg agaaatcaaa
    24421 tgacggggta acgccctgaa gggtttccac tgatactgcg acatgcgtta gtaggatgtg
    24481 catccaaacg cgtatgaagc tcgctgaagt cggcagaagg gtaccgtaag gaaagacacg
    24541 atacgtatct accacgcaag tgctctagag gtagggtatg tacctgatat gtcgggtgtc
    24601 ctaaaaagtt caataaggtg agaatgattt gattatctga caaacttgcg aatggaatgt
    24661 ctgaaacgaa taactcatag aaatgtgagg gggaaggggg atacgtcctc tttccattga
    24721 ctcgaggttg agtaaaaatg tgtttctatg aaaaaccttg catggttaaa ggctatggag
    24781 tagtcaacag gcaaaagcaa gcacctaagt tgaaatctat ggatagatag taaggaacag
    24841 gggaagcatc tagcacggag ggagcatacc gaagatgtgt ttctaaggaa aaaccgaaat
    24901 cggttataac ttaggtgaat agatgtgaaa gtagagccac gaccagtgaa aatttcgtaa
    24961 tgaaattgga ggaacaggct caagtcgtta tttataatat aagaaaccta tttaaaatca
    25021 tggattctcg taagactaaa aaggtcacga accagaatgg agcgtgataa aaccatgact
    25081 aacacaaaag gaattagctt aaagaacaaa caactcagac acgccgaata ctacgacatg
    25141 actgagactt tcgatgaatt gcacgcaatg agtgttgcaa aatcaaattt taaaaatctc
    25201 atgaaattca ttattgatga caataatatc tttctagcgt atagaaatat caaaaagaat
    25261 aaaggaagtg caacggccgg atacgatggt gtcactataa aagacattga aaaattggca
    25321 tgtggtgact ttttaactat tattaaaagg cgatttaagg attacagacc aagaaaagtt
    25381 aaaagagtag agatcccgaa accaaacgga aaacttcgac ctttaggaat tccttcaata
    25441 tgggacagga tagtccagca atgtattcta caaattttag aaccaatatg cgaagcaaaa
    25501 tttaataagc atagctatgg attcagacca aattgttcta cagaaaacgc catagcagat
    25561 tgtcttgcta gaatcaatca aagtaagatg aattatgttg tagatattga tataaaagga
    25621 ttctttgacg aagtaaacca taccaaactt atgcgtcaaa tatggacaat ggggattcga
    25681 gataaacaac tccttgtaat tattcgtaag atgttaaagg ctccgattgt tatgcgaaat
    25741 ggcaatgttg tttttccaac caagggtact ccacaaggag ggattttaag tccactgctt
    25801 gcgaatatat gcctgaatga atttgattgg tggatagcta atcaatggga ggaacgaact
    25861 gtcaaagagc tgaaacacca ctatcgaaaa aacggagcat gggataaagg aaatatctat
    25921 tcgaaattaa gaagccacac aagtttaaaa gagatgtatc tggtacgcta tgcagatgat
    25981 tttaaaattt tctgccgaac aggagaagaa gcccaaaggg ctttttatgc cacacaaaaa
    26041 tggctcagag aaagattaaa gctcccaatt tcaatggaga aatctaaagt tacgaaccta
    26101 aataaagctg aaagtgaatt cttgggtttt tcaataagac tccacaaaaa gggaaaaaag
    26161 aaagtggctt tcacgtacgt ttgtcgcaaa gcagaagaaa acattaaaaa taaactaaaa
    26221 caacagatta aaaaaattca atacgctgga gcaaaaaaaa ataggcaccg agcaataggt
    26281 gactacaaca tgatggtcat cggtatccac aattactatc ggatagcttc acattgtagt
    26341 gtcaatttca ctgagattta tagaagtgtt aggacagtaa tagaagcaag actgaaagct
    26401 agaggaatga cgaaaaatgg aatctatgga caagatcatg aaggataccg tccgtatcta
    26461 aagtctaagc aaatgagata tattgaaggt tttccgatac ttccaattgg ctatattaaa
    26521 aaaagaactc cactcaacaa gcggagaaat ctaaataaat ataccaagga agggagaaag
    26581 ctcattcatc aagagcaaca agctatagaa agctggaaaa tcaagtatct tgtaaaaggt
    26641 tttagtaaca ataagaaata cacggtggaa ttttctgata acaaaatatc taagtttatt
    26701 tcccaaaaag ggaaatgcgc tatcacagga aacgaacttt tgatggaaga aattgaatgc
    26761 catcacatta acccatatca tttgtccaaa gacgatagtt acaagaacct tgtcattatc
    26821 tctagagaaa tccatagatt aatccatgca actagtgaag aaactattct gaaactgctt
    26881 cggttactga aactaaatca ctatcaaatt agaaaactaa acacgtatcg aaaaaaagca
    26941 gctaattttc caatatcaat caaaatgaat tagataggaa acaaattata ttgtaattaa
    27001 cgatggaacg ccgtgtgcgg tgaaagtcgc acgcacggtg tggaccgggg gaaaagacgg
    27061 agataatatc aaactcttac ctatcggaat tgccaccaat ggataatatg gaaggaatca
    27121 tgacggtcac agctggccga aacatgttgt gggatctgtt tattcaatct tatcagcaac
    27181 tttatgcgaa gtatggagaa caaaacgcag cgattatcaa agaaaattgt cagacacata
    27241 tttatattat gtcgacagat gataatacca tcgaagaatt ttctaaaaaa gtggggaata
    27301 agacggttga acaagagaac agtaccatca attcattagg tatgaatact catatcaatc
    27361 gcaatgttga ttcagatcgt atcttaactc ctgaaagaat ttctactttt ttagaaggtg
    27421 aaacgattgt actcgctcct ttaaaacgac aagatttgaa aggtcataaa atacggccgt
    27481 atcctatttt taatcatggg gaaacgaatc ttccttatgc ttatcaatat attgaagatt
    27541 tcaatccatc gtgggatatc aatgatatag acatacattg tacacatgcg gacttggatc
    27601 ttaaggcttt acaaattgat tatgattatt tcctaccaga tgaatatgct agaaatctct
    27661 atcatggact gcatgatcaa ttagaagaaa aaaacgaaga aaatatgttt tataaaatcg
    27721 ttcaatctct tgagaatgag acggctaaaa aattgttaat tcaattttac gaagaacaag
    27781 cagcagatcg acttcaagaa tatgttgaaa agttgatcaa aaatgaagaa atttctgaaa
    27841 tgattgggaa acggttgtta ttggaatctg aaaaggtggt gaaagaatga tcattggagg
    27901 aattttttct ggcatagccg actggtggtc aggcttattc ggagaagggg atgcaaaccc
    27961 caaaaccgta acgaaagtct tacaagatta tggtaattat ctgcagtatc aagatgtgtt
    28021 cagttggatt tgtaatactt ttttatgggg gttgatcaaa gggttatata atctcaatac
    28081 tttactagaa aaaaccattt atcaaagctt tgatttaaaa gacttattga acgccgctgg
    28141 ggtaaatgaa ctatttcaaa gtttgattag taaggtattg gctctttttt gtgtcgtcac
    28201 attaatttat ttaggcttga aattttttat gagtaaacac ccaccaaaag tcaaaaatat
    28261 cttggttaat ttatttatgg ccatgatctt gatttttggt ggtagttctt taatcgatga
    28321 agggttaaat atcagtaaaa gcttttacgg agacgtcact gcagcaaata aagcagataa
    28381 aacgggttca cctgcttttc aaattattca aaataatgtg atcgatgtca gtacattact
    28441 agcaactgac ccgaataaag tagaatccct gccaacagac aaaagaaatg ctttaacagc
    28501 gaaaaatttt aaaacagcga atatcaatgg gattattact cctaaacaag ctaaagaaat
    28561 ggcggataaa gataagagtg gtttatcaga tttacaaaag gatcggttaa gagcgttaca
    28621 ataccgactg gaactggatg ataatggaaa agaaattcct gtgcaaatca gtgatgaagg
    28681 cttagccaaa tatgtttata cttcagggta tcgtcgatac attagtagtc cagggattat
    28741 tttagctggt gaatcttctt tggcggtggc ctatttattt atcctattta cgatcgttac
    28801 ttgcattatt gagctggtat ttaaaaaatt ctatttagtg attgccgcgt caacggactt
    28861 tgagacagga caacggatga aaagtgctat cgaagatatt tctcaatcct tcttgttatt
    28921 ggcatttaca attttagaat tacgaatcta taccttaatg ctttcaggta ttggtgattt
    28981 gcatgcggca ggaaagatca atggattctt gtatgttgtc gctttgattg tactaaccat
    29041 tgcgctattc aaaggctctc aatcagttac gaaaattttc ggcgttgata cgagtctgaa
    29101 aaacggcagt aattctcttc ttagcatgtt tgccttagga aatattgcga aaaatgtggg
    29161 tagcggagct aaaaaccttg caagtgctgg taagagtggt ttaagccatt taaatgatat
    29221 tcgtaaaaat ggatttggcc atcgaaaaga tgttacccct gataacgggg aagaacaatc
    29281 agacgataaa gctacaactg tacctaatag taagaaaaca gtttccgaaa tgttcgggaa
    29341 agcgaaaaat ggtgtttcta aagccgcaga aggtcttggt tacgtcaatg aacgtgggtt
    29401 aaaaggaaca gctgaagatc ttacagaaaa agccaaagac accttagaag ataaattaga
    29461 cggcacgaga gctaaagaat tggctgaagc tgtgaagaat ccatccgaag cactagaaaa
    29521 acaaaaagaa aaagcttcag aagtcaaaga aggactaaaa gatgcgtatg gtgaaggaca
    29581 agtcaatgca gcagtgaaag ctaatacaac aaccgaaaaa gaaaatcaag aaggtatagt
    29641 gattccttca gtcaaagatc accaagaagc gttgcactct aatgaaaatt cgattgaaga
    29701 gaaaaattcc atagaagaaa gaaattcaga agaaggaaaa aatggacagt taccaaaaac
    29761 tgatccagaa caatatcgct ataaacctaa cagtgacctt gaaaatgaga agcgaatttc
    29821 agatttatca aaagtaaatc aagaagacaa agcagaaaat gagggacagt taccaaaaac
    29881 tgatccagaa caatatcggt atagatctgt taactccgaa aatgaaaaac gcatttcaga
    29941 tttaccaaaa cgaaatcaag aagtacaaac ggatgaggat tccatcgttc tgccaaatga
    30001 taatggaatc gaagttggaa ctgaaaaatc aacgaaaatt ggtttaccta aggcacaaaa
    30061 tttaccaatg aaagatagtc gtttgaatca attcaaggat aatgatcaaa gttttgttca
    30121 tcaatcacct gcaggtactg ttcaaatgaa accaagtcgt ttgcctgaaa atggaaatct
    30181 gacaactgtt tcttacaatt tatctgaaag tggtgagaca gggaatgcgt ccgttcaaaa
    30241 tgttgtgaca agagaagctg caccaaaacc tattcaaaac gttcatacag gttacaagcc
    30301 agcaaatgtg gcatttactg caccaacaaa aataaatgtg acaagagaaa ctaaaaacag
    30361 gaacaatgca ttgaaagaac aactattgag ggatatcaaa caggatatca aaccataaaa
    30421 ggcctacaag ttaggccttt tctttatatg aaaaggagaa atcaattatg gataaatata
    30481 taaacatgaa aaaccctcca agatttagtg gagaatggaa gtttttcgct tttttctttt
    30541 tagatctatt aacgcttggt ggctgctttt tttgggcgaa aatggtcaat aatcttgtcc
    30601 acttaccact cttttttgca ctcttgaatt atggcttatg gattgcgata gcgttagtga
    30661 ttgtttggcg accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta
    30721 ttttttttgc ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta
    30781 cgtataagga ggatgaataa atgcgagtca aaggacaaaa ttcagttgaa aggaaatcca
    30841 ttttgcagtt tggaaaacca gaagagcgct ctatcttgga cttatttccg tataaatcct
    30901 gtacagaaga gggagtctta attactagag aagatcattt tcaacgttat tatcgtgtag
    30961 tttctacaga cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga
    31021 ccaccgtcat gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg
    31081 atctaacgga acaaatcgtg caaaagcggc agttattaga taaaaatcgt atggaacagt
    31141 ccttaggaag aaatctacag cgattaagaa aatatgaaaa gaaattagta gaggaaatcc
    31201 aggaattgaa acgagcagaa caggaacgcc ctgatttaag cttctttttc gtcattgaag
    31261 gaaagacaat caaggatttg caaaccagaa accgtcagtt aatgcgatct agtggcattt
    31321 tagggttaaa accactctac aaaaaagaat tgatcaagat tctttatcga atgaataata
    31381 tgaatgatga gtaggaatgg ggaagagaga atgaaatatc cgttgatatg gttagaaaaa
    31441 gatgttccga aagaagagac cactagaaaa tttcctcaca ctttaagcct gggtaagttg
    31501 ggaaaaggaa aatcttttaa ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt
    31561 tagaagcaat taaaacagct ttttcaccac aaaatgatca aaaggtactt gatccattaa
    31621 agcaactagg aaatcgggaa atagaaattt taaaatccaa agggtatgat ttagactttt
    31681 taaaacaagt gatgccacaa ggtggattaa atttcgatga agagtatgtg acctatggaa
    31741 atggttattc acgatgtgtg acagtttatc actacacaaa aaatcctacg cttttttggt
    31801 tggccaatct catgaacaat cctcatacag tggcaacact agatgttagc acagatgaaa
    31861 agcaaaaagt gattgatcaa atcaatcgtg ccttagatga attaaatgat cggtcagata
    31921 atgaacgaaa agcgactgac cgaaataaaa gcgcggaaga atacctagaa ctagcggaat
    31981 atgcccgaaa attgacttct aatggggaaa tttctaaaca agtcaaagtg agagtacatg
    32041 tttacggaaa cacgattcaa gaagtagatg aacgagcaca ggaactgatc gaaaatctca
    32101 aagggatgga ttatcgtgca gtgacctatc tgcacaaaac gaaaagagac tggcaatcga
    32161 tgttcaccat gttcgatgaa caagctgaat ggtttggaaa tatcaaaggg caatcactac
    32221 catcgctaaa cattggcgga ggtttcccgt tccaccacca aacgctaaaa gatccaggtg
    32281 gaacacaata tggggaaacc atgacaggcg gcccgtttgt ttggaatgcc acgtataaaa
    32341 atagtgtgcg gttgtctacc aataccttgc ttctaggaat gatgggagct ggtaaatcga
    32401 ccgttcttaa aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg
    32461 aaaagggaaa agactttatt ccctttttaa aggaatataa cggcattatg gtgcgtttag
    32521 acggatctga cgggatgatc aatcctttag aaatatttgc cacacgtacg tatgatgaag
    32581 cttccagttt atatgacgat ggatcagtca aagatttgaa gatcaatgaa gcagcgtcct
    32641 atcaaacgca cttagacaaa gtggtctatc aggtacagtt agtgagtcct cagctaaaag
    32701 gaacgatgaa agcagaattt aagacctatt taaatcgttt ttatgaagag tatggaacag
    32761 taccacgcgg ctttacttcc tcaaattcgc gttcaaatac tgatacgcaa gtcacaggta
    32821 aagatccaga agcttatccg acgttcaaag aatttttaga ctttttagga caactggaat
    32881 taccaggtgc ttcccaagag aaaaagaatc gaaaagaaga aatggaatcc atcgtagaat
    32941 ccttatgtga gacgtatggc atgatttttg atggtcatag tacgattcga caccttgacc
    33001 aacaacagtt ggtctttttt gacattgact ctattacagg cttagatcga ggggtacaac
    33061 aatgtcaaat gtacatggct atgacgttga tttggaatca ggcattgatt caaggaagaa
    33121 gacaacgacg tttaattaaa gaaaaacaaa tggaagaaca agacttcaaa tgcttcttgg
    33181 cgatgattga tgagtgccat aatgcgatta atccagattt tattgaaacg gtaaattatg
    33241 tcacaaactt tcaacgagaa atgcgtaaag tagaagcaat gactgtttta gctacacaat
    33301 ccccacaaga aatggtgcca gaaaatatgt cttctgataa cttctcgaaa ctgaagaaag
    33361 tctttgaatt ttcttctacg aagatcttta tgcgtatgga tgaatctatt ttacgtcata
    33421 tagaaggatt agttggtaaa tcaatgacaa actcagaatt agaatccatt cctcaacaaa
    33481 atcaaggaga cgctgtgatc aattttggct caaaagaaac cattcgtgtt cattttagtc
    33541 cgaacaaacg tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc aaaatgaaag
    33601 tttaatcaag cagtatgtta agcgtcgtgc aaagcgacgc ttatttttgt ggttgtttgg
    33661 tacttctgca ggtttgatta ctattttgat tactgtgttc gtgacccttt ttctaatact
    33721 tgcggcgggt tcgattgata attctgattc agattcaagt agtggtggcg aagcttttac
    33781 aggagaatat tcggaaggct taccgattta taaagaaatc aaaggaagag gccctttttc
    33841 tgatgagatt gcgcaatatg ctgtaggtgc agcagtgaaa tataaattat taccaagtgt
    33901 gatcctttcg caatacggtt atgaatcagc gtttggtacg tcggcgtccg caagaaatga
    33961 tctcaactat tttgggatta cttggtttga tggttgtctg tttcctaaag gaactgcgcg
    34021 aggaatcggt ggtattgaag gtggctggta catgaagttt cccaactcaa aagcagcctt
    34081 ctcctattac ggctttatgg tagccacaca atccaatttt aatgcatgtg taggaaataa
    34141 aagccctggc gcaagcttat tgatcttagg acgaggtggt tatgccgctg caggaattac
    34201 tgaagatagt ccgtattata caggttgtat gtccattatc acttctaata aattaaccga
    34261 atatgatgaa ttcgccatca aacattgggg tgaaggtggc aataacaacg gcacgatcac
    34321 tggtgaatgg acgaatccat tccctggcag cagtttagat aaaagttcct tcagtggtgg
    34381 tcaattattt ggaacaaatc caggcggtga attccgtccg aatggcttcc atgatggatt
    34441 agactttggt tcagtcgatc atccaggaag tgaaattcac gcggtgcatg gaggaaaagt
    34501 cgtgtacgtt ggcaatcctg gtatttcagg tttaggtgca tgtgtgatcg tgattaatta
    34561 cgacggtttg aatatggtat atcaagaatt tgcgaatagt acaggaaatt ctcgtgtgaa
    34621 ggttggcgat caagtgaaag ttggtcaagt aatcgggata cgagatacag cacacttgca
    34681 tttaggtttc actcgaatgg attggcgtca agcacaagga cacgcattca cagacgatgg
    34741 tacatggatt gatccattac catctttgaa tagcagcaaa aaatagatca ggagaaaaaa
    34801 tatggaaaat aaaactttaa aaatcgtctt aacagctgca attagcgtta gtttaatcgg
    34861 caatatcgcg ttgttttggc agctgaaaga agaacaagag acaaatacct ttaaaattga
    34921 agaagtggct aaaaacgcgt ctgagaagct caaaaaaaat tcctctgagg cacaggcgaa
    34981 gatcgacaag ttaaatcaaa ccatcaatga gaaaaaccaa gaaatcgatc agctgaagca
    35041 acaagatggc acaaaagcaa aagaggaagt aactgaagca caaagaaaga cggcagaaga
    35101 gttcggtcaa ttggccattg ataaatcatt aagtagaaat gaattaacgg aaaaactaaa
    35161 agatgtcgca acccaagaag tgatcgacaa attaagtcct gcagacgatg atcaccaaca
    35221 tgatgattac ggaagttact cctttacatt aggtgacgtg caaacttatg ctcaaacaag
    35281 cagtgtaaaa gaggaacaaa attttgtcgt atttgttgat tatacaatcg gcaatccgca
    35341 attcaaagat gtcaaaccac aaaaaataaa aggtggatta actgtcactg aaaaacaagt
    35401 agatggtcaa tggaaagtca tagactttag ttatttcact cgttaggagg aagaaaatga
    35461 gtaagaaaaa aggagaaaga caacgtttca aaaaacgaaa aagttatctt caatggctgg
    35521 gaatctttat gattctcggc tttttgattc ttgctagtcc gatttatacg aatcagaagg
    35581 tagatatacc agatgaggta agcacgaacg cctttttaaa cacgaaagag ctgacaatgg
    35641 tttcaaaaga aaaaggagcg aaccagcaat tagtttttga atttgcgatc gatgatgaag
    35701 atcaaagctt gttaaaagaa ctggctaatc ttaattatcg cgtagaagta aagacagcta
    35761 aaggggatta tcaagcaata cgagccaaag tgatcaaggt gtctgatgat tattttgcag
    35821 tgaagctaac gcatgtacca gaagaatata tcgccatgcg tttaaccatt attcctgaaa
    35881 aaattgatcc aaaagtggat atgcaagaac cacaagatct gatttattac gtgcacgaag
    35941 ataaggttaa agatcaagtg aaagatgaag attatgaaca acacgcgatc aattacaaag
    36001 tgaaaggata caagaaagaa caaaaagcag ctcaaaaaca aatggaatct ttccaagcaa
    36061 ccattgattt gaatgaagaa ttagtgaaga agctaaaaga tcaattgcct tatcaagtcg
    36121 cagatgatca ggaaaatact gaaaacaaga ttaatagcta ccagcaagaa attgaaacat
    36181 cgaaaacgca actgaaagat caagaagaga tcattcaaaa acttcaagaa aaaattgatc
    36241 gtatgacaaa agaaaagatt tagaaggaca ggtggagtga aaaaatgaag cgaacaaact
    36301 tggctgacaa agatactttc gctgagcaga aagtattgat taaagcctat ctcaaagttg
    36361 taaaagctaa agaaatatgt cgagaagtga atttatctaa cacaacaata tctgagttga
    36421 agaatggtcg aaagaattta gatactttaa aattcagatc atttgaagct ctatatgtag
    36481 ctgctaaaaa atattttaca gctgataaca aataaaaaac aggagtattt tatgtgaaaa
    36541 aagtatataa aaaatgctta tgaatcagct atatcacctt aaccatttgt taatgagatg
    36601 ggagaaaaca ttaaccacga ttctaacgca aacatcaagc acataaaaca tttaacactc
    36661 ctcctttctg gctgaagatt tcaaccaaac aggaagacag cgtatcaatt atagcataca
    36721 ctttttctta aattcatttt tcatatgaca aaatatttcc ttctaatcga caaagttaga
    36781 aggatttttt attgtctaaa tacacaaata accttaattt ttaaagagca taaggaaacg
    36841 tgatatcact ttctcacgaa aggtgttagg taggctgtgc ctaccacgtt tctaagaaag
    36901 ataaatgata tcaaaatttt ggatttgtgt gtagatttgt gggtaggatg aaagcgaatg
    36961 tgggcagtaa aataagcgtt tgtgtgtagc aaaatcaaaa aatgtgtagg gatatgtgta
    37021 gctacacata tccctacaca taataaattt ggaggaaaat atggacttat taattagaaa
    37081 tattgaagaa aagtatatta ataaaattga caaacgttgt gaagaactta cggtcaagac
    37141 tggcaaaaaa tggagtcgaa atcaatacct aaaagttttg atcgaaaatg attttgatca
    37201 cgctcttctg aattataaaa aagaccaatt tgatcggttg ttagaaaaat ttgtggatat
    37261 tcaaacttat aatacgaaaa ctttagaaga atatattgca acaaataatc aattaattgg
    37321 tttgttaatt gaataataaa aatagtattg tgaatttata aaaggaggaa atcaattttg
    37381 gcaaaagcaa caagaaaaca tgtatatctt tacgatatgc atatcgaagt acttgatgaa
    37441 ataatttcta ctcatgaaga tatatctaat tatagtgaag cagtcagaca tttgtgtatg
    37501 atgtatgatc caaaaatcga agaaaaaata aatccaattc aaattaaatt gaatgcaatg
    37561 ggaaaagaaa tttctatttt aaccgaaatg gtttcagagt ttgcggatct tcatttgaaa
    37621 gatacaggaa tactaattgg tacaaattca actgtttatc aagaagcaga agaacgtgtg
    37681 gaaggaaaaa ttaaatccaa tcaaaccaaa aaatattctt ctaagttaaa gaaaccagtg
    37741 aatgaatctg ttcataccag agtagatcca tttgatccaa cgaagattct aaaaagataa
    37801 aagtagaagg agaacgtgat gaataaatcg cctgcagtta ttcttacttg tcaatttacc
    37861 ttgcccaata aaaaatcttt ttcaacatac attgattata tgacaagaga aaaagcactg
    37921 gaagaaatgg aacgacgtac gcccgaagaa gaacaggaac tgcaacgaat caagaatgct
    37981 ttagaggatt tttatattcc tgaaggagaa actttttcgg aaagtaagac ggataaagaa
    38041 ttcagtgaaa cgtcgaatga agctcaatcg ttgatggaac atggaatcaa ttttgacaag
    38101 ttagaggaac aagattttac caaatatctt tcttatatga cccgtcacta tgcgttagaa
    38161 caaaagaaag aactgacgca aagggaacaa aaagaatacc aacggttgaa ccaagcaatg
    38221 aaaaaatatc aaacagagcc acataaaaaa gcttcaggaa atcttcctgg agttttttct
    38281 atggcttcag acgaagtgaa aggaagtgat ctgaaggaga tcaaacaaat cttccaaaga
    38341 ggacaacaaa aaggctcaat catttatcaa gatgttatct ctcacgacaa ttcgtatctt
    38401 gaaaaaatgg gcttgtacga tcccaaaaca gataccttag atgaagaagg tttgaaagca
    38461 gctggaagaa aaatgatgga gacattattc gagaaagaac aattgaatga aacaggttac
    38521 tggatggcaa cgattcaccg taacactaag catatccata ttcattttgc agttgttgaa
    38581 aaagaaaata cgcgtaaacc gtatactgag attgaaaatg gtgtgagtta tatcgtaccg
    38641 aaggggaaga gaagacaagc aacattagac gcaatgaaaa ccaattatgt acatgagtta
    38701 gaacgtttct cttatgaaaa ggatcgaatg atcggtatgg agaaacgaaa tttattgaca
    38761 cgaaaaagtg atttgcgtaa caccttaaca aaagtagtaa aagaaccagc acgttacgat
    38821 cgacacgcca tgaacttatt aaatgaagta tataaaaccc tacctgaaaa acgttcggaa
    38881 tggaattatg gagatgaaaa tcgaaccaag ctttcaccat ttacaagggg aaaacttgat
    38941 gagttaacca aatatgtttt aacaaaagaa cctggatatc aagaatatat acagatttca
    39001 aaagcattga aagaagaagc caaaagcctt tatggggaaa gcaaaagaga aacaaagaat
    39061 gcagaaaaaa acgccctctt tgatttaaaa aagcgcacag gaaatgcgat tttaacagaa
    39121 ctgaagagac aggatagtca aataaaacca ttaatagaaa gtgtcaatca gatgcaatct
    39181 atttacaatg aacgatcctt tcgaacactc aaacagttgc caacaaatga atacaagtat
    39241 agttatcaag aattcgtaaa gcgtaggaaa caattccaac aaagagttgt cacgcagcga
    39301 agcttgcgtc aattaaatcg aacaattaat gcacatgaag aaaaatacca cgcaagtaaa
    39361 gcatatgaag agcaacagcg aagaatccaa atggaacaag aaagacaata ggaggaagag
    39421 aaatgaaaca atatttagat caatggaaag tcatagaagg atcactaaaa gaagaacgaa
    39481 tcgaacaact tcctgattgt ttagaaaaag agcatttgtt tcagataagc gaaatgttga
    39541 gaaacgaaca gtttgatcca aatcaatttt tagttgttga gtacccagct actggtgtgt
    39601 attgttgtaa tcatgtgaag ggtgaaaaat attttattat tcaagaatac gaaggtaagt
    39661 tagcgcctta ttatactact tgggaaataa atgaagaggg aatcaataat ttcccatgtg
    39721 aaagcattga agagtctatt tctctcacgg aatgttaaat aaatagagac gtaaaagagt
    39781 cctgaacgtg attgtccagg actctttttc aatgaattgt tttccgccaa ttcactgagt
    39841 ggtgcgattc aattttatta tatcaaaaga aagtgagatt ccaaagtgaa gggaggaaga
    39901 agaaatgact cagacaacga ttggtgttgt ggttgaaaaa agcagggata atctcgtttt
    39961 tgtgacagaa attcaaacag gtagagcttt tgtagtcaca gacaaagctg caaaagcgta
    40021 tcaaaatggc aatattttaa cgttaaatct gactacgaag atgttcgttg atgcagtaga
    40081 aaattatcca tttgtttaga caaacgatat tttacaagga ggaaacggac atggcagaag
    40141 aaaaagaacc actagataat agtcgtttgg gaaagtccaa aagaaaatta gtacggttgc
    40201 aaaacgaatt aaataatcaa tttcaaaagc tatttgatca ccaaaaattg acaaatgggc
    40261 aaccaatgaa tgacaaacga aatggtcgtt catggtttaa ccagcaagaa aaaatagaaa
    40321 ataaaataca aagtttgaga gaagagatca agcaacaaga aaaacaggta gaaaaacttg
    40381 aaagacaaga agagctaaag gaaatggg
//
LOCUS       DACDNO010000020        37265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDNO010000020 DACDNO010000000
VERSION     DACDNO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.020"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            260..598
                     /locus_tag="IWI22_001110"
     CDS             260..598
                     /locus_tag="IWI22_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168515.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(803..1042)
                     /locus_tag="IWI22_001111"
     CDS             complement(803..1042)
                     /locus_tag="IWI22_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP9168516.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1018..1365)
                     /locus_tag="IWI22_001112"
     CDS             complement(1018..1365)
                     /locus_tag="IWI22_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9168517.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2097..2678)
                     /locus_tag="IWI22_001113"
     CDS             complement(2097..2678)
                     /locus_tag="IWI22_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9168518.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2861..3487)
                     /locus_tag="IWI22_001114"
     CDS             complement(2861..3487)
                     /locus_tag="IWI22_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9168519.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3509..4840)
                     /locus_tag="IWI22_001115"
     CDS             complement(3509..4840)
                     /locus_tag="IWI22_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9168520.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4995..5396)
                     /locus_tag="IWI22_001116"
     CDS             complement(4995..5396)
                     /locus_tag="IWI22_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9168521.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5482..5688)
                     /locus_tag="IWI22_001117"
     CDS             complement(5482..5688)
                     /locus_tag="IWI22_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168522.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5783..7285)
                     /locus_tag="IWI22_001118"
     CDS             complement(5783..7285)
                     /locus_tag="IWI22_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9168523.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7303..7758)
                     /gene="argR"
                     /locus_tag="IWI22_001119"
     CDS             complement(7303..7758)
                     /gene="argR"
                     /locus_tag="IWI22_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9168524.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7856..8473)
                     /locus_tag="IWI22_001120"
     CDS             complement(7856..8473)
                     /locus_tag="IWI22_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168525.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8694..10067
                     /locus_tag="IWI22_001121"
     CDS             8694..10067
                     /locus_tag="IWI22_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9168526.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10142..11254
                     /locus_tag="IWI22_001122"
     CDS             10142..11254
                     /locus_tag="IWI22_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9168527.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11326..12963)
                     /locus_tag="IWI22_001123"
     CDS             complement(11326..12963)
                     /locus_tag="IWI22_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9168528.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13131..13739)
                     /locus_tag="IWI22_001124"
     CDS             complement(13131..13739)
                     /locus_tag="IWI22_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9168529.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14123..15274)
                     /gene="queG"
                     /locus_tag="IWI22_001125"
     CDS             complement(14123..15274)
                     /gene="queG"
                     /locus_tag="IWI22_001125"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9168530.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15293..16045)
                     /locus_tag="IWI22_001126"
     CDS             complement(15293..16045)
                     /locus_tag="IWI22_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168531.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16186..17136)
                     /locus_tag="IWI22_001127"
     CDS             complement(16186..17136)
                     /locus_tag="IWI22_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9168532.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17137..17976)
                     /locus_tag="IWI22_001128"
     CDS             complement(17137..17976)
                     /locus_tag="IWI22_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9168533.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17973..18719)
                     /locus_tag="IWI22_001129"
     CDS             complement(17973..18719)
                     /locus_tag="IWI22_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9168534.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18957..20327
                     /locus_tag="IWI22_001130"
     CDS             18957..20327
                     /locus_tag="IWI22_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9168535.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20382..21278)
                     /locus_tag="IWI22_001131"
     CDS             complement(20382..21278)
                     /locus_tag="IWI22_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9168536.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21559..22236)
                     /locus_tag="IWI22_001132"
     CDS             complement(21559..22236)
                     /locus_tag="IWI22_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9168537.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22327..23232)
                     /locus_tag="IWI22_001133"
     CDS             complement(22327..23232)
                     /locus_tag="IWI22_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9168538.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23407..24831
                     /locus_tag="IWI22_001134"
     CDS             23407..24831
                     /locus_tag="IWI22_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9168539.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            24877..25908
                     /locus_tag="IWI22_001135"
     CDS             24877..25908
                     /locus_tag="IWI22_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9168540.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25915..27102
                     /locus_tag="IWI22_001136"
     CDS             25915..27102
                     /locus_tag="IWI22_001136"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9168541.1"
                     /translation="MEIKRSLNTKFAEIFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27258..28640
                     /locus_tag="IWI22_001137"
     CDS             27258..28640
                     /locus_tag="IWI22_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9168542.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28612..28998
                     /locus_tag="IWI22_001138"
     CDS             28612..28998
                     /locus_tag="IWI22_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168543.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29198..29866)
                     /locus_tag="IWI22_001139"
     CDS             complement(29198..29866)
                     /locus_tag="IWI22_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9168544.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(30055..31481)
                     /locus_tag="IWI22_001140"
     CDS             complement(join(30055..30908,30908..31481))
                     /locus_tag="IWI22_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9168545.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSLVQFRKTTVTVV"
     gene            31724..32179
                     /gene="tnpA"
                     /locus_tag="IWI22_001141"
     CDS             31724..32179
                     /gene="tnpA"
                     /locus_tag="IWI22_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9168546.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            32444..32542
                     /locus_tag="IWI22_001142"
                     /pseudo
     CDS             32444..32542
                     /locus_tag="IWI22_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(32765..32995)
                     /locus_tag="IWI22_001143"
     CDS             complement(32765..32995)
                     /locus_tag="IWI22_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9168547.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(33225..34043)
                     /locus_tag="IWI22_001144"
     CDS             complement(33225..34043)
                     /locus_tag="IWI22_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9168548.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            34204..34893
                     /locus_tag="IWI22_001145"
     CDS             34204..34893
                     /locus_tag="IWI22_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9168549.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            34907..36409
                     /locus_tag="IWI22_001146"
     CDS             34907..36409
                     /locus_tag="IWI22_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9168550.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            36422..36898
                     /locus_tag="IWI22_001147"
     CDS             36422..36898
                     /locus_tag="IWI22_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9168551.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(36995..37105)
                     /locus_tag="IWI22_001148"
                     /pseudo
     CDS             complement(36995..37105)
                     /locus_tag="IWI22_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10767 a         7289 c         6787 g        12422 t
ORIGIN      
        1 ctatataaca ctaagtaacg caaaattccc ctttgtaaac tatctagaaa attgttttgt
       61 ttaaagtata tcttattgga tataaataga aataaattgt agccagttca ttttttcttt
      121 aactaaaaaa ataatccaag aaacattgaa cctatggatc caaaagctac ccaaatacta
      181 tagctaaccc cccgctacct tcaattttgc agataaaacg ctttgggaga aaatttcaaa
      241 gacaacaggc catcctatca tgcccgatta taaacaaaaa gctggctatt ccttttgctc
      301 tgacaaattt caattgggaa agttcacgaa actagcaggt gaagaagttt caactgttcg
      361 gatcaaagaa tgcccgcttc aaatagaaac attggttcaa cgaatttcaa ctagacaaga
      421 ctttgctatt gtagaacgtg agatcgtagc tatttttgta gaagaaggga ttttacatga
      481 tccaactcat atcaatgtgg ataaatggca acctcttatc tataaattca gagaatatac
      541 aactgccaac cagtcgttaa ctttgaattt tagattccaa gaattcaaag agtcataatg
      601 ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac gagtaaaaaa caaatagaag
      661 ctatacatta gaaaggtcga ctgtgaccct atagatgcag tatatgcagc ttatgagcat
      721 ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc tttttcgttt tttgccgctc
      781 tttctttgaa aaatcgatta tgttatacct tctccattac taattcaggc atctgctgag
      841 taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg ttcgatttgt tcttcagtca
      901 agacaagcgg cattcggtca tggattggtg cgactgtgcg gttaggttca gtagtcatta
      961 tgattgattc tgccattttt tcatcctgat ggctacgata aaaaccgcca atatacacta
     1021 ttttttcctt atttgaaaac ataaatttgt ctttttcttc attccactca ttgggaaaac
     1081 acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg tctctgcacg
     1141 ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca tgatttccgg
     1201 agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg gaaaaacttc
     1261 tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc gataataatt
     1321 ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa tccctcccaa
     1381 tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc tttcaatttt
     1441 tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta ttctcctgcc
     1501 aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt ttgaaattgt
     1561 tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg tgttcaaaag
     1621 ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagctatt ttcacaaaat
     1681 ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa cggtgttcaa
     1741 aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc tatttttatt
     1801 tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac taaacggtgt
     1861 tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc
     1921 cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa tacacggtgt
     1981 tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga gaagcaattt
     2041 tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc taattattaa
     2101 agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg cctaagtagt
     2161 gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc aatcaacgct
     2221 ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc caggatattt
     2281 tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg gtattcttca
     2341 tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata agtgatctgt
     2401 gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc tggctgtttt
     2461 ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg cgcatggatc
     2521 accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc ttttcctgta
     2581 tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt ataaataaga
     2641 tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct ttttcatttc
     2701 tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt ttaaaaaaga
     2761 ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata gctagatgtt
     2821 cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt tgctctaata
     2881 cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac gctgcatcca
     2941 ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg aatccactct
     3001 ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca ccaagaatta
     3061 ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct ggtccaaaga
     3121 ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat tcttcgataa
     3181 aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca aacatctgct
     3241 gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca tgtgggtaaa
     3301 aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt accatttcaa
     3361 tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca aagccaccgc
     3421 ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta tttttattat
     3481 tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga gcttcactca
     3541 ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt ccattcatag
     3601 ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt gcccctagaa
     3661 tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct tctaatactt
     3721 gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg ccttgtgctt
     3781 tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat acactgtaag
     3841 gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga tccataatga
     3901 ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg tctccaatcg
     3961 cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct ccgcgatctg
     4021 ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt cttccagttg
     4081 cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta aaggtgacgg
     4141 cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg aattcgattc
     4201 ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca cgtggaagaa
     4261 attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac attgatgcaa
     4321 attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat tgttcctgat
     4381 ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg attggtaaaa
     4441 tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc actttttcac
     4501 cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg tccatcactg
     4561 tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg cccgtcaatt
     4621 tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg aaaatcaatg
     4681 atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt gttgactttt
     4741 caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc ttaccgccct
     4801 ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg cttctttttt
     4861 acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca aatcatctgc
     4921 tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag tctggttcga
     4981 tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct agttgctttg
     5041 gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc ttcgctgatg
     5101 ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc taaattataa
     5161 agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt gatcatatgt
     5221 tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc tggatttgtt
     5281 ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt tggtgcctcg
     5341 acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt tttcattgta
     5401 ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg acaagtaagg
     5461 cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta ctatttttcc
     5521 aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa aataaatagc
     5581 aatcttttct acataatctt ttatatgatt gattttaaga tacatatata ccagaaagtt
     5641 gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt tttcctcctt
     5701 ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc gtccaagcaa
     5761 ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca gaaaaatgtc
     5821 cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca tcattttcta
     5881 tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag ccgtttccgt
     5941 atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag ttcatccctt
     6001 tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt tcgcgtggtt
     6061 catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc aactctttga
     6121 tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc aggatctttg
     6181 cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg tgtccgcttg
     6241 tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct ttattgatta
     6301 catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca atgaatcttt
     6361 gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc tcttttaata
     6421 cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca gaagcactga
     6481 caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct tcatttgcta
     6541 aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg atatgattag
     6601 gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct aagctattca
     6661 atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg attttccaac
     6721 catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg actaaatctt
     6781 gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg ctttttattt
     6841 tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct tctgaggtaa
     6901 agaattttat tccattataa taatagggaa gatggctagc agtcagcata accgcagcat
     6961 cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta gctaattcga
     7021 aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct ttcagtgctg
     7081 gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt ggatcggttg
     7141 atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt accatgtctg
     7201 tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata tccgatccat
     7261 tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc tcattttttc
     7321 cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa tgaatgtatc
     7381 tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc aattcgttac
     7441 cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag actggatacg
     7501 caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa cagatggctg
     7561 gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta acttcatttc
     7621 tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat gcagccgcgc
     7681 aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga tcagtgcttg
     7741 tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa agataagtaa
     7801 tatttcttca tattttggta ttttttactt ttattttttg ttttcattca ggttgctaac
     7861 ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat tttcttggat
     7921 cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct tttgcgatga
     7981 atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg tgtaagattt
     8041 gggcacaaag accttcttcc atctttttga atactacttg ttctttcaag tcttccggaa
     8101 gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag tcaggctgtc
     8161 ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc aaatactttt
     8221 cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa tcaggaatca
     8281 gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga caggctactc
     8341 ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag aatgattttt
     8401 taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct ttacgccatt
     8461 caattttttc catttttctt cctccgcgaa attccattta gttatttatt atcatacctc
     8521 tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct agaataagtg
     8581 aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc ggtttctatt
     8641 tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg taaatgacaa
     8701 aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg tcttttgcgg
     8761 gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga cagtttgatt
     8821 taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt tcgatcattg
     8881 ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt ttagcttcaa
     8941 atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc atggtcctat
     9001 tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg atgttccata
     9061 ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc ggtactttga
     9121 caacggccat tgctccagca attggcccca cttatggagg cattatgact tcacatttta
     9181 catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg attatcggct
     9241 ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg ataagcgttt
     9301 taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg ggaacagttt
     9361 ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac tatcgaagta
     9421 aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca ttccgcttgt
     9481 ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt gtcttgccga
     9541 atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg atgcttcccg
     9601 gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat caatatggtc
     9661 cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta gcattattcc
     9721 tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt tatatgatcg
     9781 gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg aatgaagaac
     9841 tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc gctgtggcta
     9901 cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat aactatgaag
     9961 tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg cttctcgtaa
    10021 gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt ccatatacgg
    10081 caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag gaggcttttt
    10141 tatggaaaaa attgccatta tcggtggcgg gatcattggt atgaccctag ccagttactt
    10201 agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg caactaaagc
    10261 tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat ggtatcgtct
    10321 ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac ttgatcaaac
    10381 gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag ctgaattggc
    10441 tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg aatggctttc
    10501 acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac tgcgtatcag
    10561 tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc gtttagcaca
    10621 aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg attcttggct
    10681 catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac ctggcccggc
    10741 acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc aaaaagggca
    10801 gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg ctatgcttga
    10861 cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg ctacccatga
    10921 aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat tgactaagag
    10981 tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg agttccgtgt
    11041 aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc cagatgatcc
    11101 aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc cttatatcgg
    11161 ctatctatta gccaacttct taaatactgg tcattggcct acggaagatt atcaaaagga
    11221 catctccacc tatattacga aaaatccatc atagaaaaac agctttttca agtaaggatc
    11281 ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt tcaatttctg
    11341 aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc ctatctgagc
    11401 ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat aggacgtatc
    11461 ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa atggaccaaa
    11521 cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg tcgtttcagg
    11581 tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg tttgctgttt
    11641 caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac ggaaaggatt
    11701 agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat tttccgcgtc
    11761 tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt cattgatctt
    11821 gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga tcttttcgct
    11881 aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc catttccgtt
    11941 tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca cgcccacatt
    12001 ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt gtgttttatc
    12061 aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa cgaaagcatc
    12121 gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa tcgttgattt
    12181 tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac tagcatattg
    12241 tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc aagcaagcgt
    12301 caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg ctgtattatg
    12361 gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt cgggatcaca
    12421 aaagatataa agatctccct catcaaagga aacagacttt tgataagtct ctaagatctt
    12481 cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa atccttctgg
    12541 aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta cttttgtatg
    12601 gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct cgtatttgct
    12661 ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc ccacaatgct
    12721 tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt cagcatgtga
    12781 gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat aagctgtctt
    12841 cctatcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa tcgatcgaat
    12901 actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct cttttgtact
    12961 catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca ttattcgagt
    13021 ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac aaaaaaaccg
    13081 agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc ttagtaccag
    13141 ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga tgttacatat
    13201 tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta tgaagcatct
    13261 aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc tgattctttt
    13321 tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc tggggcagca
    13381 ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt tgctgtttca
    13441 gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga atcatttgaa
    13501 gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat cgaatttttt
    13561 tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat cttagataat
    13621 gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt tcctacaaac
    13681 attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa tgaattcata
    13741 ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt atcatttaga
    13801 aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat tatttaatat
    13861 ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg ttgtataagc
    13921 aaagtaatcg tttttctatg tttttttatt tttgaatgaa aaacgcagat tctgtaacat
    13981 tcactgcaat aatccataaa aaaaagcaaa aaaatttttg atttttatca aattctctta
    14041 aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca acaattactg gacaaaaact
    14101 ttgaataatg ttaactattt ttttatttta actttctccg caataatttc tgggcatgtt
    14161 ccaattcgtt tataacgact tcttctgttt ctttagctaa tatttcatct aatacatcaa
    14221 gccatttttc cggttctttt gttcctagac gtccaatagc ccaagctgct gttcctctga
    14281 tgacagggcg gacatctgtt aaacattctg aaatctcagg caatgccgtt cgatcaccta
    14341 gattagctaa agcaatcaat gcgtttctct gcaaaggttt cttcccacgc catgaaccag
    14401 ctaaatgccc aaattgctgt ttgaagtctt tattagacat cgaaagcatt ggctttaatt
    14461 ttggatatat ttcgtctact tttggttcca tctcttcatg aaaatgaaaa tcttttcctt
    14521 gattgtaagg gcagactaac tggcagatat cacatccata aatgacattt cgcatttttt
    14581 tacgatattc ttccggcatc atcccttttg tctgagtttg ataagacaaa catcgctggg
    14641 cattcattct tccgtcacca agcaatgctt ttgtcggaca agcagtcacg cagcgcatac
    14701 agtcaccgca accaaattct cctggtttat caggctcaaa tacaatatta gtaacgatct
    14761 caccaagata gacaaatgaa ccgaactctt cagtgatcaa caatccgttt cgtccaataa
    14821 atcctaaacc agctcgctgt gctacagcga catcgatcag ttctccagta tctacttgcg
    14881 gagctagacg ccattcctct tctttttgcc atttttctgc tcttgtttgg atgaattcga
    14941 tcaatcggtt caaacggtct ctgagtatat catggtaatc gatcccccaa gaagcacgag
    15001 caaactgccc gcgtttttca tccctaggca cttcttcatg gatcctagta ggatacgcaa
    15061 gtgcaatgga aatgatcgat ttcggctgat caaatgtcat ttctggataa agacgctcat
    15121 cgatatttgg atgttcaaag ccagaagtat ggccagctgc tttttgttct tccaacgatt
    15181 cttttaatga atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt cctaatcgtt
    15241 tgctttcttg tatgatttct tcttttaatg tcatggtctt cctcgcttgc tgttaacggc
    15301 tgtataaccc ataatatagt ttttcttttt tcagctggac aaactggatc tgctgatcgg
    15361 caatcttctt ttcttccatc catttttttc ttgcagcaaa tacttcccaa taatttgttg
    15421 aaattaattt tcttttgtta tctaatgcat taaaaaaatt caatgaaact ggttttgtac
    15481 ttaacagttc atggagcctt acctcttccc atggcgaaaa ctcagacaga tcgcgggaat
    15541 acaatacttc ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc gggattttcc
    15601 caggcctagc aatttctact agacgattat aaaacatatt cattaatttg ctgttggcaa
    15661 taaattgact tttataatag gccgactgga tctttttgat tactccttct gctttgatga
    15721 ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc gttttgttta actaatacgc
    15781 caggtgtgaa gccgactgga gaaaggaaca caaaataacc gataccggta ttttcatcta
    15841 aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag ctcttcatct ggttctgttg
    15901 tctcattttg atttgctatg aataatttag ctaattctgc ttcaagaaat tgaaggtctt
    15961 gatccttgtc ggtattttcc tgctggctga tcaatcgaaa gacttcttct ttaatcgtat
    16021 atttatcttt acgtgtaaaa ttcatagcca caccttcctt ccttactcta actataacgg
    16081 aatttcttca aaaaaggtat ctgtacgacg aaaaacagca taattttttt tcatatctct
    16141 tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc acttttcagc tttcttttgt
    16201 ttgtgacaaa ccttcatgga tcgtttcaat attccatttc atcatctgat agtaagaatc
    16261 tccttgttct ccttcttttg ctatggaatc agtgaacagt ttatcataga ttttcaaacc
    16321 cgtttctttt gctacccgtt ccatgcttct tgagtctaca ctggtttcca cgaataaaac
    16381 cggtacttct tttgctctta tctgatcaat gatcgctttc atttgatcag gcgtaccttg
    16441 actttctgta ttgatctccc atatataagc tgctggcaaa tcatatgctc gtgaaaaata
    16501 cttgaatgcc ccttcacttg ttactaataa ttttttgttc tcttcgatag aagcaaaact
    16561 ttccttcgct tgtgtatcta attcttttag tttttccaca tatgcttctg catttttttt
    16621 atagagtgtc gcattttctg catctttttc agagaatata cgtgcgattt cctccacata
    16681 ttttattccg ttagataggt ctagccaagc atggggatct gcttttgtat gttcttcacc
    16741 gctagttaaa taaagaggtt ctacattttt gctaactgca aaataatctt ttccttcttc
    16801 ttttttagcc gtttccatca agttatcgaa ccagctgtta cctgtttcaa gattcaaacc
    16861 gttgtataaa ataacatctg catcgcttgc ctttttgatg tcttctggta gtacttcata
    16921 ttcatgcgga tctgttccga ctggtacgat actgtggata tctatttgat ctgttccgac
    16981 ctcttttgcc atgtccgcca agatcgaatt ggtagccacg actgacaact tttctttact
    17041 tccttcatcc gcttggtcag aagcctgctt tccgcaaccg gctaatacca agaaactaac
    17101 tgccaagatt aaaaagagtg atttttttgt tttcatctat ctgcttcctt tctgaaataa
    17161 aattcctttt gttggagaaa ataaaaaagc aaggataaaa aatgcggtac atgctaacac
    17221 gatcgtagct cctgatggcc aattgtaact ataactgaaa aacacaccga caactgcact
    17281 tagcataccg attcctgcag ctgtaatgat cattttcagt aaatggttcg ttagcaagta
    17341 agccgtggcg gctggtgtga tcaacatggc aatcaccaag attgttccta ctgtctgtaa
    17401 actgactaca gccactaatg tcaaaaagaa catcaaaaga taatggatca gcgatgtgtt
    17461 cagcccataa gcttttgcca ttgtcggatc gaatgatgtg atcttcaatt ctttataaaa
    17521 gaaaaagaca aatatcaaga cgattccact aacgagtgct gtcagcaaca aatcgctttc
    17581 cctaacagct aagacattcc caaataaaat atgatacaaa tctgtcgcac tctgggcata
    17641 tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag acaattccaa tcgctgtatc
    17701 atttttcaat cggctccgct gagtcacgaa accaatcact gctgctgtta gcatcccaaa
    17761 agcagtggct ccaaagatgt aactaaaacc aaacatataa gaaatggcta cacctggaag
    17821 aactgcgtga gagatagcgt ctcccatcaa agacatccct cgtaaaataa taaaagaacc
    17881 gatgactcca gaagcaagtc ctacaagcat ggaagtgacc aaagcgtatt gcaggaattg
    17941 ataattcatt aatccatcaa taaaggattg gatcattttt tctctcctcc ttttactaaa
    18001 tgtctgatga tctcaccata ggccaattga aggttcttcg atgtaaagac ttcttgtact
    18061 ggaccggaag cgatcagctg tttatttaaa agaataacct catcgaaata ttcttctact
    18121 ttatgaagat catgatggac gatcaaaatc gtttttcctg aattcttcaa ttcctgcaag
    18181 atgtcaaaga tctttcgttc acttaacgca tcaatcccta cgaatggttc atctaaaaag
    18241 atccattctg ctccttgggc tagagctctt gcaataaaaa ctctctgaag ctgtccaccc
    18301 gatagttcgc tgatctgtct tttggcatat tcttccatcc ctactttttt caaagcttgt
    18361 cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa ttcgtaaaga tggatatgtt
    18421 cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa gatccaattc acttcgctgt
    18481 tccacgtagg ctattttttt acggactgag tttaccggct gtccagaaaa ggtgacttta
    18541 ccagtagtag ctggtatcaa ctctagcaaa gatttcataa atgttgattt tcctgcaccg
    18601 tttggtccga taataccggt aattttttgt ggatttatcg tcaagttgat agttttcaaa
    18661 acagttttcc cttgataagc ggctgaaagg tcttgaatta caatggccgt acgttccaaa
    18721 tcttacactc ctttttaggt ttacctaact ttttatttaa atatagttta actaaaatgg
    18781 aaagcatttt caagtagaat gatgaaaaag tttttttcga attttcctcg cattttatga
    18841 aaattttctg tagaatcaca tcaagagttc aataaggcta acctaataat gaaaaacatg
    18901 tgttgaaacg tttgattatt ttttcttgtt gactcaacaa cgaaaggagt ttttgtatgg
    18961 aagaagaaaa gttaactcga agtcttagtg cccgtcatat ccaaatgatc gcactaggtg
    19021 gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac catacgttgg acagggccct
    19081 cagtgatggg agcctatgcg attgcaggat tctttttgta tttgattatg cgtgctttag
    19141 gcgaaatgct ttacgttgat ccttcgactg gttcttttgc aaattacgcg agcgagtaca
    19201 tccatcctgt ggctggctac ttgaccgctt ggagtaatat ttttcaatat attgtcgtag
    19261 gaataagtga agtgattgct gtaggatctt atatgaatta ttggtggcct gatttgccgg
    19321 caattattcc cggaatcatt gtcgtcttgt ttcttatgct ggcaaatctt atttctgtca
    19381 aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa agtcataacg atcattttga
    19441 tgatcattgc aggtcttggg gtaatcttat ttggatttgg taatcatggt caggcagtag
    19501 ggatttcaaa tctttggaaa aatggtggat tcttcactgg aggcgtcaaa ggatttttct
    19561 ttgcattatc gatcgttgtt gcttcctatc aaggaatcga actgatcgga atgactgctg
    19621 gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt ccagtccact atcggtcgaa
    19681 tcttgatttt ttatattggt gctattttta tcattgtgag tatctatccg tggaatcaat
    19741 tgagtgagat tggttcacct tttgtacaaa ctttttctaa agtcggcatt acatttgctg
    19801 caggactgat taattttgtc gtgattacag cggcattatc aggttgcaat tcagggattt
    19861 tcagcgcaag tcgtatggtc tatacacttg ccataaatgg aaagatgtct aaaaaatttc
    19921 tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt agcaatctca ggtggaatct
    19981 taattggtct gatcttaaat tatcttctac caatgttcat caaccagtca gaaaatattt
    20041 tcgttttcgt atacagctca agtattttgc ctggtatggt tccttgggta gtgattttga
    20101 tcagcgagat tcgcttcaaa aagaagcacg cagataaaat gcatgatcat ccttttaaaa
    20161 tgccactatc tccttatacg aattatgtga cacttgcttt tttagctatc gtattgattt
    20221 tcatgtttat caatcctgaa acgagaatct cattgattat tggactcgtt ttcttgcttt
    20281 acatgacgat ccattacttt gtacgcgaaa aaagaaccgg aaaataaaaa aagaacgaag
    20341 cttcacctta gtggaagctt cgttcttttt tattgctttt atcaattcaa ttttagcgtc
    20401 tcaccgggag tcaggaaata attctccatg ctcatcccat tcagcgacaa gatcgtttct
    20461 actggtacgc cagttcgttc ggctatttga tttggacctt ctccttccaa aactacggtt
    20521 gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag aggaatctgt tgtgtcttgt
    20581 gttgttcgag cttgcatttc agaagcttct accgactctg ctgtctcaga atcagaagat
    20641 gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg cgctagtctg caaataaata
    20701 tcggaaatct tagcgacatg attcgtataa ttagccgata attgattgat caaagtatct
    20761 aagtctacac cgtctttatt ttctgctgcc tgatacgctg cacgtaattt tctgatccct
    20821 tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag caactgctga taaaccagct
    20881 attccctctt gatttccctg aatttctgtc tggtattctt caatcaatgc aggcgtcttt
    20941 tcttcgatct ttttcgaata atcatttacg attgcttgaa gttgctctgg caaaccattt
    21001 gctgtttttt cttcagaagt ttgtgtctct tttgttttag ttgatgactc acttgtttgt
    21061 ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg tacttttcgc ttctgacgaa
    21121 ttttttccat gaaaatatag acccgcaact cctcctgcta tgatcaaaaa cacggcaact
    21181 gctgccccaa tcatgatcat ccgcttacga gctgcctgtt tttgctgtct tcttcgggtc
    21241 atccgaacat tttctcccca ctcattttca tcatacattt ttttcaccac caatttttat
    21301 gtataatagc gttatcaaac gcttcatatt cttattgtag caaataattg tcgctgtgca
    21361 catttttatc tttgttattt ataagaaaat ttcaccagtt gagatgattc caagattgtc
    21421 cgtcgatttc tccttgcagc tgcattgttc gttcgtctat ctcagccatc agtttcttta
    21481 tttcatataa gagcgcacgg tcttgatatt ctgatttttc gcttaattca tctaattgtt
    21541 ggatcagcca atcatttgtc ataatgaagt tcctttcatt tcttgcagtg taaaggtcaa
    21601 gtcttcgatc tgctcttttg cctcagtcag tgcttctacg atatatgctt gtttttcaga
    21661 ttcttcttcc tgcattttct gattttcctg tactctttga ataaaccagt caatccgctg
    21721 agtcagcgat ggtttgcctt ccaatgagag atactgctga taagcaatta gcttctccca
    21781 acgtttttcc tgggataaat acataaattg tgctaaaacg aatggcggaa gataggtcat
    21841 atggaattta tttgcgattc gctgatacgg cctcaaaaat tcggaaatcg tatattcttc
    21901 ttttcctcca gcttggtatt ctttttccgc cacacctgtt gtcaatacga ttcctagttc
    21961 tttccctttt aatacatgtg ctgctcgttc caagacatta tcttgccaaa tttttaaatg
    22021 agcaggagaa ctataccagt aaaacggaaa ctggaaaatg atccgatcat gctgggccaa
    22081 gagctgtagt tcagtctcta tatctatttt tccgtctgga taacttgatt ccaaatgatg
    22141 ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat tgctgattta aagattcttc
    22201 gatcgttgga tggctaatga tgataagtgt acgcatccct tttcctcctt tttagtcttc
    22261 gctttacagt gtagcataac cgccacaagt ttcttttgcc gatgcttgtc attttaacca
    22321 gactaatcag gttgtccaag aaactgcggt ttgtttttcc aactgttttt tcaaggtatc
    22381 caaagcaata ttcttaaaat tttcgttttt catacgttta cgatagttcg tagccgtttc
    22441 tccggtactc tttttgaaca ttcggctaaa tacgactaga ctattgaacc ctacttgata
    22501 ggcaatactt tccacggatt catccgtcag agaaagaaga aacgcccctc gattgatccg
    22561 taccccgatc aaatattcct tgatactggt gcccatatga tttttgaata ctcgcgacaa
    22621 atggctccga ttgaccgcta aatgctgggc gatttcctca acagacaatt tttcagcata
    22681 atgtgcctcg atgtatttga ctgtttcaac agctaaagag gaatgaacct tttgtacacc
    22741 tccgctgacc tttccgccat cttttaaaag gactgataaa aaacggtagg ttaatgctgt
    22801 taactgtaat tcattttcca aggtttcttc cgtatctttc atgcattcta aaataatgtc
    22861 gatatatttt tgtatgtcag aagagaccat cgtgtattgg tcttctctga aaagcgactg
    22921 ccgaatgatt tcttctgcag cactcccacc aaatgaaagc cagcaataca tccacggatc
    22981 ttctccatct gataagtaaa acgtcgagtc tcctggacga atcaagaaca gatcaccttt
    23041 tttcaattgg aattgttgat ttttcacatg ataagatcct ttgccttcca tcacgacatg
    23101 caggatgtaa tcattgcgaa tagcaggacc aaaagaatga aacggcatcg ttttagagaa
    23161 accacaaaaa tcaaaataca attctttttc tacttgttga tgttcctttt ttaaaaaaat
    23221 cgattgctcc acgtctatcc ctccttactc ctttagtata atgcaacaaa aagttaaata
    23281 aaagtcacag aaatatatta aaaaggaagc gatttcttgt taagtttaag tcattaaaag
    23341 atctttgtta ttcgttgtaa ccggtttact tagtattata tattgaacag gagaggaata
    23401 cagattatga aaagcaaaat gatccaacgt gtgtcctacg ctttaggcgc ttttggacac
    23461 gacacattct atgttacatt aagcacttat tttatggtat tcgtgacaag ccagatgttc
    23521 gcaggaacag ataaggcaac gaatgcaaaa atgattgcta cagttacaag cttagttgta
    23581 ggtatccgct tagttgaaat tatttttgat cctttgattg gtggcatcat cgataatact
    23641 cgtacaaaat acggtaaatt caaaccatgg ttagttatcg gcggcgttgt tagttccgta
    23701 ctcttggtag caatctttac caatttcttt ggcttagcta ctcataatac gactttattt
    23761 atcatattat ttgcaatcgt tttcatctta ttggatagtt tttattcatt taaggatatc
    23821 gctttttggt caatgattcc agctttgagt acggaaacaa aggaacgtga aaaatttgct
    23881 acgtttgctc gtttcggctc ttctctaggt gcaaatggta ctactctagt cgttgtccct
    23941 atcgttactt tctttaccta tcttgttact cataaacata ttgaaggcgc ttcaggctgg
    24001 ttctggttcg gtgtgattgc tgctgttatt tcaggcggta ctgcgattat cactgccttg
    24061 ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg aaaaagctgg gatcaaagat
    24121 gtgttcaaag cagttgctca aaatgatcag ttgatgtggc ttgcccttgc ttatatcgct
    24181 tatgcgattg ccaatgcagc aacaactgcc gtattattct acttcttcaa atatgtactt
    24241 ggccaaccaa ctgaattttg gatcgttggt ctagttgctg ccttacttgg tttagtagct
    24301 gttccactat tcccaatgct tacacgcgtc atctctcgtc gctacgttta ccttggaggg
    24361 atcgcaatga tgatttcttc ttactttttc tttacgatcg caggttcaag tttgccaatc
    24421 gttatcatcg ggctagtatt gttctacttc cctcaacaat tgattttctt atccgctttg
    24481 atgacgatca cagactctgt tgaatatggt caatggaaaa atggtatccg taacgaagct
    24541 gttactctat caattcgccc tttactagat aaaatcgctg gtgctttgtc taacgggatc
    24601 gtagggtttg tcgctatcgc ttctgggatg actggtaatg ctactgctgc tgatatcact
    24661 gctcatggcg taacgacttt caaagcttat gcattctatg ctcctgctgc tttgatgatt
    24721 ttctccgcta ttatttttgc ttggaagatt acattgacag agaaaaaaca tgcacaaatc
    24781 gttgagaaat tggaaaacaa attggctcca gctgatacaa tggaagatta acttcacgat
    24841 gcagtagttc cttcccatta agaaaggatc ttgaaaatga agattacaga aaaagatttt
    24901 ggccaaggtt atcacttgat aaccttggag aacaaaaaca agctagcact ttctatctct
    24961 gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc gagaattagt tttgggattt
    25021 gacaccgctg aagaatatat cgaaaaagat ccgtatatcg gtgcttctat cggtcgcacc
    25081 gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa aaacttacca attagccaca
    25141 gatccaaaaa ctggacataa tctgcatggt ggtgctcctg gatttgaatt aaaaaaatgg
    25201 tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca tttttactac tacaagtccc
    25261 gacggagaac atggttttcc aggtacaatg gatgtggaaa tccgctatac tttgacaaaa
    25321 gataatatct ggcgagtgac ctctcgcggc accagtgacc aagatacttt attcaatccg
    25381 acaaatcatg tttacttcaa cttgacggga gatgcttctc agtcaatcga tcaacatgag
    25441 ctttggctca atagcgaagc ttatgctcct ttgcgaacag atagtattcc tataggggta
    25501 aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac ctaaaaaatt agcgtccgtt
    25561 tttgctagtg atttggatca aaaaaatctt gttgacggaa tcgatcatcc atttttctta
    25621 aaagaaaccg gcttgggtaa agaagccgct cgcttgacta gccccgataa aagaatccaa
    25681 gtagatattg ctacagatgc ttctagtgtg gttattttca ctgcaaattt cggcacagaa
    25741 acaccagaaa tgcgcaaccg aaaactggca catcacggtg gcatcacatt tgagactcaa
    25801 acagctccag gtgcagagcg cttctcagca tttggttcga ttcatttaaa agccggatca
    25861 gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa aggaataact catcatggaa
    25921 atcaaaagaa gcttaaacac aaaatttgcc gaaatattcg gacctaaaaa aactacacaa
    25981 tacttctcac caggacgcat caatttgatt ggtgaacaca cagattacaa tggaggccat
    26041 gtcttccccg cttccatcac ttatggaacg caaggtgtag cagcacctcg agacgataat
    26101 aaaatattag tttattccac aaactttgac gatatcggtg tcattagttt tactttagat
    26161 gagctagaat atgataaaaa agcaaactgg gcaaattacg taaaaggaat ggtccttaaa
    26221 ttaaaagaag ctggacactc aatcgatcat gggtttgagt tattagtgga aggaacaatc
    26281 cctaatggtg caggactttc cagtagcgct tcattagaat tattagtagg tgttgtatta
    26341 gaagatctgt ttgatttaaa aacagatcgc ttggaactag tccaaacagg taaaaaagta
    26401 gaaaatgaat tcatcggtgt caattcaggt atcatggatc agtttgccat cggcttcggt
    26461 gagctagatc atgcaatctt attagataca aatacgctga aatacgaaat ggttccagtg
    26521 aaactagatg gttatgcaat cgtcatcatg aatacgaata agcggcggga attggcagat
    26581 tcaaaataca atgaacgccg tagcgaatgc gaagaagcct tggcacgttt gcaaaaaact
    26641 ttgcctatct cttctttagg agacttagat gaagaagaat ttgaatcaac tattgaccaa
    26701 atcggcgatg atacattgat acgcagagct cgccatgccg tttctgaaaa tcagcggact
    26761 ttgaaggcaa aagctgaatt agaagctggg aacttagaag catttggtca actgttaaat
    26821 gattctcacc actctcttcg ctatgattat gaagtaactg ggatcgaatt agatacacta
    26881 gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac gaatgacagg agctggattt
    26941 ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac ctgccttcaa aaacaatgtc
    27001 tatgatgaat acatgaaagt tgtcggctat gctccggaat tttatgttgc acacattgga
    27061 aatgggacaa caaagatcga tccagaagct gttcatgttt gataaaaaaa tcaacgatct
    27121 cagctaaaaa caaatagata aaaataacct ctcagcaatt tccaaatgtg agccttttgc
    27181 tcgtatttga aaattgctgt tttttattga cacggaaaga gaacgtgtgt tctaattgtg
    27241 ttgaagaggt gattgttatg tgggcgaatc aaaatccggt atttgtttat gaaaaagaac
    27301 cttcaagaga tattttatgt attgactgca aatcttttta cgcctccgta gagtgcgttg
    27361 aaaaggatct tgatcccctt acaactaaat tagtagtcat gtcctatccg tccgattcga
    27421 cagaaacacg gggtagcgga ttaatcttgg cttccagtcc tgcagcaaaa aaagcttacg
    27481 gcatcaccaa tatcagccgt gccagagatc ttcccttccc ctatccaagt gatttatata
    27541 ttgttgcccc aaggatggcc tattatatgg aaaagaacag agaaatcaat tctatctata
    27601 aaaaatatgc agacgaagcg aaccatcatg tctattccgt cgatgaaagc ttccttgatg
    27661 taacaaatag catggcactc tttggtaaaa caaatgcgta cgacatggct aaactgatcc
    27721 ataaagatgt gtttgagcag accggaatct acacgactat aggaattggc gataacccgc
    27781 tacttgctaa actagcctta gacaatggct caaaagacgg aaagcaaatg tttgctgaat
    27841 ggcgttatga agatgtacca gaaaagttat ggaaaatctc agatatcact gacttttggg
    27901 gaatcggcaa acgaacagca aaacgtctga aacaaatggg tatacattct gtttatgatt
    27961 tagctcacgc ggattattat cacttgaaaa gcaagctagg ggtcatcggt actcagcttt
    28021 atgcccatgc ttggggcatc gatcgaagtt ttttgggaca agaatataca ccaaaatcaa
    28081 aaagtatcgg aaatagtcaa atcctagata gagattatac aagacgagaa gaaatcgaga
    28141 tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt gagacgatct gcagcaaaaa
    28201 cacaagtagt cagcttatgg gtaggatact ctttaggcta tcttgatcca aatggcaaga
    28261 ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc tagcagagat ctagcagatg
    28321 ctttattgac cattttccac cagcattacc actaccagga tattcggcgt gtaggcgtga
    28381 actgttccga cctcgtatac gcgacaggtt tacagcttaa cctttttgaa gatcctgaaa
    28441 agcagataaa tgaagcgaaa gttgattttg ttgtcgatac gattagaaaa aaatacggct
    28501 ttcaatcaat catccatgcg catagtctct tgcaaggagg acgcgccatc gcacgtagtt
    28561 ctctcgttgg cgggcatgca ggtggtatga gtggattaga aggtggaaaa gatgagacgg
    28621 acagcaaaaa aattcattga ctataacgag tatcgagatc ggccatttgg attaaaatgg
    28681 gggactgctt ttgcgatgga agaactagtc aacggtatca aaaaaaatga agaagaagca
    28741 cgcaaacagc ctcttcctaa acaacaaatg acattgcaag aaatcgatag tatactgaca
    28801 gaagcctttt tatctcgtaa agcagtaatc attcagctca atttaaaaga tgaatttgga
    28861 agactcttgg atgaggtcga agggcacttc attggtgaag cctatgaaga ttactttatg
    28921 attgaccatc agccgatatt gtgggaagac gtacggtata ttgagttaaa ggaaacaaaa
    28981 aaatggtttg tgacatgaga ggttgtgaca gaagtgatca acttcaaaaa ataagaaagg
    29041 atttttcgaa aattgcttct taaatttttg tggaattttg gcttattttt cgaagaagca
    29101 acttctggaa caacgtatat tagatatcag aggttgtgaa aaagtcgctt agttttgagt
    29161 aacaagccaa ggcgctgatt tcacaacctc tgtctatcta tccatctatt cggtaagatt
    29221 catgcatgac gtcttcttta tttagtatgt agagcccttt ttcttctgac cattcaacat
    29281 aaatcagctg ttttacatct gcatagcctt ctttcttcaa gttctccata agccatgctt
    29341 cagtcaattg attggactga agcactttat gctgaatttg cccttcatct acaagcagga
    29401 tcgataaagc tttgtcttca tctgattttt tcaatacact catttgaccg gcattctcta
    29461 ggataacatg tttggcattt tcaagaaaaa agcattcttg ctgtcttaag attgctcgca
    29521 actgctccat ctcgatatgg ttatttgtca actctgtcat gttgatgacg ccgtctttga
    29581 ttagaacaga tggctcgcct ttgacccaac gattgacctt ttcatttttt tggacgatac
    29641 gttcgatacc ataaatcatt actgcccaaa gcgcaatggc aaataggaca tgccctacat
    29701 ggacattgtc atcataggtt gattcttcta agatcccacc taaaacaagc gtatagacta
    29761 aatcaaacgg tgtgatttct gacatggatt tcttcccaag caaacgtacc actaataata
    29821 aaccgatcaa cccgaacacc agtttcattg taattgaaaa aatcattttt tctcccccta
    29881 actatctgtt aattaaatta tatatgcttt ctcacgaaca cgcatggaat atacaaggaa
    29941 gaaatgtatc cttttactaa aaaaaggact gtgacactgt agtgaccccc aaaagttaga
    30001 cttttttgga gtggagaaat ccactccttt tttttatgct ctaaaatgat atttttacac
    30061 aaccgttacg gttgttttac gaaattggac gagactttgc cagtttaatt tctgttttat
    30121 ccgttcatta ttgtagtaat agatatagct atcgatggct gattttaatt cctcgaaaca
    30181 gttataaact ttcccgtgaa aaatctcttg ttttaataag ccaaaaaaat tctccataag
    30241 tgaattatct aagcagttcc ctttccgaga catgctttga aagattttgt actccttcaa
    30301 tttgtttcta taagctttca tttgatacgc ccaaccttga tctgagtgaa acgtacgacg
    30361 atagggacaa tcgttagtgg tctggatagc ttcctccaaa ccttccatta ccgctaatgc
    30421 attgggcttt tcagagatac gataggataa aatctcacta ttgaacatat ccataaaagg
    30481 atctagatac aatttctttt gacggataat gcctttggca tccacttcat ggtatttaaa
    30541 ttcagttgta tccgtcgtga ttttctgatg gcagatactg gtgtagaaac gacgatggat
    30601 ccgatttttg gctactgctc cgacttgtcc tcgataagaa ttgtagcgcc gtgatttacg
    30661 tgtgtatgtc gttactttta ttcctagctt cttgatcagg cgctgaactt tctttttatt
    30721 gatagagaaa ccgcggtttc ttagttcatt ggtcatccgt aaacagccat aatccttgtg
    30781 cttctcccga actttaagca tttccgcttc aatctcttga tctggatttt tacggtccaa
    30841 tcgtttttgc cagtacataa agattgcctt agggaacttt aaagtttcta gaagctcaac
    30901 taatttaaac ggtcctcgga ggcttgcgat gattcgtgcg attgcttcat tcgtcgttgt
    30961 tgggcttcct gttttcgcag cttcctcaat tcttttaaaa aggcattttc aatttgaagg
    31021 gaacggactc gtttctccaa ctcctcgatg cgttcacgtt ctttagacgt tgctttagtt
    31081 gtagattttt tcttattttt gggttcattt ttcttagaca agataggtgg acatcctttc
    31141 gtttttgaga gtccatcgat cccaccctca cggaattgac gcatccaact agcgatcaaa
    31201 ttagggttgt tcatgttaag tgtgttggca acttcacgat aggacagctc acttgttaga
    31261 tataactcta tcgcatccag cttaaattga acagaatatt ttttcttttg tcgctttctt
    31321 actagaccct cttcaccaaa ttcttgatag gcattgatcc agcgcttcac ttgtgatgga
    31381 gccttaatgg aatatttctt tgccagataa tcacttccgc ctttaccatc caagtaatca
    31441 tgaacgatct ttaatttaag ttcaaaagta tacttagcca taaaaaatac cgacctccaa
    31501 aagttagatt ttttggtcta acttttgggg gtcggtacaa aacttaaaag ttttagaccc
    31561 ttttattttc ttaatgactt cagtcagttt tgtgcgtaaa gcgtgcgaaa caagaaaacc
    31621 gcgcgtttct acgcgtggaa gaagtatctt tagcgataaa aactcctttc gatatactag
    31681 tgagtggcta ccaaccgctc atcagatcga aaggagtttt ttcatgtcaa atgacgataa
    31741 aagtttagca tatactcgtt ggaattgtaa gtatcatatt atttttacac caaaatatca
    31801 acgaaaagta atctatgggc aattacgaaa agatcttgga aaaatactta ggaagttgtg
    31861 cgaaatgaaa gatgtggaaa tcatagaagc acatgcaatg ctagatcata ttcacatgct
    31921 tgttcgagta ccaccaaaaa tatgtatatc cagttttatg gggtatctga aaggtcgtag
    31981 tgcggtacta atccacgagc agcacgcaaa tctcaaatat aaatacggaa acaaatcctt
    32041 ttggtcgaag agatactatg tgagtacagt cggattgaat caaaagacaa atgaaaaata
    32101 catccgtgaa caagaagctg atgatcgcat tcgatatatt ataagtaaaa gagagtacgt
    32161 agaaccattt aaaaaataaa gagaagtaag tgagtgaaga tgagcagttg gcggtctttt
    32221 aaaaatcccc ttttaagggg cagcgagtgt cgaccccttc tagggggatt aaaaatacac
    32281 ccgtcctcac gggtgtatgg gttactgata gacttgattt taactggtgt cagaaactta
    32341 tgtccagaaa gggtgatgaa attttggcaa tttacggcta ttcaagaatt agcacaagca
    32401 accaagatta caaaacacaa attcaaacat tagaaaatgc tggcgcagaa ataatcttcc
    32461 tagaaaatca ctggattatc gaacaccttt ggaagtattt ttgagttacg taagtattga
    32521 tgatctgtct aacttaattt gacaattaag agtatattta cttcagatgg taaggcaaca
    32581 aaataaaata caataggcag atgaactaat aaataaaatc tgtattaaat acagataaat
    32641 taatataaaa atcaaaagga tcataaactt ttaacaagtg atgaaatggg tggaaattga
    32701 ttcttataga cagtttgtct cataacattg aagtattttg caattttagc attgaaacac
    32761 ttttttaatc ttcaacgttg ttcttataaa caagatgatt gggaatcttt tgaattaaca
    32821 ctgtatagct tagaacaccc tttatagaaa acattcgctt agcaatctta ataattcctt
    32881 gtgcccgatg tcgtttagat tcagtaggtt cctgctcatc gatcatggaa aaaatttgaa
    32941 ggatcagatc cttaataaat ttatttaaga gaggatgatc cattgcttca tccatactcg
    33001 ataaactagt gataatgagc tacacgtttt tctttcaagt tgatcaatag cttccacgat
    33061 aaaaatatct ttagataaaa tcaagaaccg taggaactac agggatattt ggtcaataag
    33121 agccaccgct attagtaaaa aataagctat caagtatgct ctcatcccaa gaattgccta
    33181 cttcaatcgt taaaaaccgt taggtttagc taagtggggg tagttcactc aatactttta
    33241 tcaataatag agcgcaaaac tttttctctt aattcaactg aatcaatatt attgttttgt
    33301 tcataaccat gaactaatag atcacaaata taaagttgtg atatttttcc agctaaagat
    33361 ccaccattaa gaaattcttc aatggctgtt tgtaaaacta agtctgcaga ctttcctata
    33421 ggtgaatgaa tatagttagt tatagcaata atttttgcgc catttttttt agctattttc
    33481 aatgaatcat aagtatcttt cgttttccca gataaagaaa aaatgattac taaatcatta
    33541 actgttaata aagaagcgac ttgggcttga aaatgagggt ctagtacagc ttttgcttga
    33601 actcctacgc gaagaaacat attttctaaa tcaagactag tatttccact agatcccact
    33661 ccaaatatat atatgttttt tgattgagtg atcattttaa tggcttcatt caatttttct
    33721 tcgtctaaaa gcctaatagt ggaatagaga gcttctgtta aactattagc aatttcatca
    33781 tagtacttta cactagtcgg atgttctttt ttttgactaa agtcttcctt tgcaatttca
    33841 atttttaaat cagaaaaacc agaaaaacca attttttgac aaaagcgaat aatagtagca
    33901 tcgccaactt ttgtgttttt cttaatatca ctcatcgtac tataaataat agttttccca
    33961 gaatttaaaa taaaatcagc gacttttttt tcagacttcg ttaaaaatgg ataattttct
    34021 ttaattaaat agatataact catacaaact cctctacaat ttcaactttt ctccattata
    34081 ctccaaaata ataaaaaaaa ctcctaaata ctccattttt atctttactt ttatgaaagc
    34141 ggatgctata ttgtttttgg agttattatc caaaaataat aaaaaattgg agcgtttagg
    34201 ataatggata aaaagaggtt tttagatctt atttcaaaag gtttgattgt ttcatgtcaa
    34261 gcattacctg gtgaaccatt ttacaaagag gaagaaagtt taatgcctta ttttgctttg
    34321 gctgctcaaa gggctggagc agttggtata cgtgcaaatt ctgttcgaga tattattgaa
    34381 attagaaagt gtgtatcatt accaatgatt ggaattatta aaagagatta tccaccagaa
    34441 tctccattca ttactgccac tatgagagaa gtagatgaat tagtagaaac cggtgttgag
    34501 gtaatcgctc ttgattgcac tcttagaaat cgacatgatg gactatctat aaattctttt
    34561 attactaaaa taaaagaaaa atatcctcat cagttattta tggcagatat ttctacattt
    34621 gaagaaggca aaaatgcata taaagcagga atagattttg taggaactac tttaagtgga
    34681 tatactgagg aaagtaaaaa acaagatggg ccagatatag atttgattaa aaaattggta
    34741 gaagaaggga tttgtgtaat tgctgaggga aggattcata ctccagaaca agcaaaagag
    34801 ataaaaaaaa taggagttgc aggtattgtt gtgggtggtg ctattactcg accacaggaa
    34861 attgcatcaa gatttgttaa agcattaagt tagggggaga aataaaatgt ttaaaaaatt
    34921 ttcacaatta ggacgcgctt tcatgttacc aattgctata ttaccagtag ccggattact
    34981 attgggactg ggaggagcgt taacaaatga gagtgctata aatgcttatc catttttaga
    35041 tcaaccgtgg ttgcatacaa ttttaagtat tatgagttat gctggaaatg cagtttttgc
    35101 aaacctagca ctaatttttg caattggtat tgctgtagga cttgcaaatg gagataaagg
    35161 aacagctggt ttagctggtg gagtagctta tttagtatat actgcaacta ttagtgggtt
    35221 tcttgaactt ttttcagcaa aagatgcaac actagataca ggtgtagtgg gatcaattgt
    35281 tgttggtggt actgttgcat ttttacacaa tcgttataga aaaattgaat taccacaatt
    35341 cttaggtttt tttggaggat caagatttat tccgattgtt tctagtttag ctgcaatcat
    35401 tattggttct ttcttttatt taatttggcc accaattcaa aatggtttag tagttgctgg
    35461 tgaacaaatt gcccaaatgg gtagtttagg aactttttta tatggttttt tattgcgttt
    35521 aacaggtgct gttggactac accacaccat ttaccctttg ttctggtata catctttagg
    35581 tggtaccgaa actgtagctg gatcaacaat tgctggagcc caaaatattt tctttgccca
    35641 actagctgat ccaaaccaca ctggtctgtt tacttatggt actagatttt ttgcaggaag
    35701 atttgcaaca atgatgtttg ggttaccagc agcatgttat gcaatgtatc gtgcaattcc
    35761 taagaaaaat cgtaaaaaga atggtgggct ttattttagc ggggccctta catcattttt
    35821 aactggaatt actgaaccag ttgaatatat gtttttattt gttgctcctt ggctttatgt
    35881 gattcatgca ttcttagatg gattatcttt ttattttgct gatatcttaa atattcgtgt
    35941 agggaattcc ttttcaggtg gtttgatcga ctttttatta ttcggtgtac ttcaagggaa
    36001 tgacaaaact aattggttaa aattaattcc ttttggtatt gcatgggcat ttatatattt
    36061 ctttgttttt acattcttta tcaaaaaatt taaggtagct attcctggaa tggaagaaga
    36121 atcagaagtt cattctgaaa aagtaataaa caaaaataca tcattacatg aagagtctct
    36181 tatcattatt gaagctcttg gtggaaaaga aaatattgag tctgttgaag cttgtgcaac
    36241 tcgtttgcgt gttgctgtta cgaatgggga gattattcaa aaacaaacaa taaaagacac
    36301 tggtgcaact gctgtcttcg aagtgaaggg tggcatccaa gctgtttttg gagggaaagc
    36361 tgacttatta agtcaagaaa tcaatcaaat attaggaaaa gataattagg aggtttttaa
    36421 tatgtttgaa attttcaaaa agaaaaaaca agaaactttt tatgcacctt gttccggaaa
    36481 aatcatttcc atcacagaag taaatgatcc tgtcttttca caaaaattac taggtgatgg
    36541 ttttgccgtc atacctgatg gttcaaaaat ttatgctcca ttggctggtc gagtaacaaa
    36601 tatatttcct actaaacatg ctattaatat aaaaagcaac tcaggcattg attatttgat
    36661 tcatattgga attgatacag ttgaattaca aggagagcct ttttcgattt gtgttcaaga
    36721 aaatgatgag gttactcctg aaacctcgtt ggttgatgtg gattttgaca aaataataaa
    36781 atctggtaaa gatacttctg ttatagttgt ttttccggag aaaaatcagt tcaatgattt
    36841 aataatagat aatggtcgaa ttgctcatgg gaattcatgt ggaaaaatca ctttttaaaa
    36901 aagtgtatat aaaaagctat aaaataagct aaataggata tttgtcaaat gaagttggtg
    36961 aacagaaaga actgttttct ttctgttcat ttttttattt tattagtaat gattccccat
    37021 gccaaatcat gatccgtaat ttaaagttat aaaagcttcg aaatccattg gcatttcgtt
    37081 ggaatacctt gatatgatta ttcaaatata aaatatagag agaagagaga gagggaagat
    37141 atttataaag ggcaaagtta tgtttactca acttaggaaa tagaaataga tcaatttata
    37201 ttctcttgtc aatcgaatat gaaccttagc actatatacc aaatatagta aaatgctgaa
    37261 agatg
//
LOCUS       DACDNO010000021        36567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDNO010000021 DACDNO010000000
VERSION     DACDNO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.021"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWI22_001149"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IWI22_001149"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9168552.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWI22_001150"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IWI22_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9168553.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWI22_001151"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IWI22_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9168554.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWI22_001152"
     CDS             complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IWI22_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9168555.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWI22_001153"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IWI22_001153"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9168556.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWI22_001154"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IWI22_001154"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9168557.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="IWI22_001155"
     CDS             complement(8052..8522)
                     /locus_tag="IWI22_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9168558.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="IWI22_001156"
     CDS             complement(8568..8993)
                     /locus_tag="IWI22_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9168559.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="IWI22_001157"
     CDS             complement(9060..9329)
                     /locus_tag="IWI22_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9168560.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="IWI22_001158"
     CDS             complement(9340..10935)
                     /locus_tag="IWI22_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9168561.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWI22_001159"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IWI22_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9168562.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="IWI22_001160"
     CDS             complement(14488..15048)
                     /locus_tag="IWI22_001160"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9168563.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="IWI22_001161"
     CDS             15401..16375
                     /locus_tag="IWI22_001161"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9168564.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="IWI22_001162"
     CDS             16545..17945
                     /locus_tag="IWI22_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9168565.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="IWI22_001163"
     CDS             complement(18023..18745)
                     /locus_tag="IWI22_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9168566.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="IWI22_001164"
     CDS             complement(18824..19330)
                     /locus_tag="IWI22_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9168567.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="IWI22_001165"
     CDS             19453..21252
                     /locus_tag="IWI22_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9168568.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="IWI22_001166"
     CDS             complement(21320..22894)
                     /locus_tag="IWI22_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168569.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="IWI22_001167"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="IWI22_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9168570.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="IWI22_001168"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="IWI22_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9168571.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="IWI22_001169"
     CDS             complement(24671..25309)
                     /locus_tag="IWI22_001169"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9168572.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="IWI22_001170"
     CDS             complement(25310..25960)
                     /locus_tag="IWI22_001170"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9168573.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="IWI22_001171"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="IWI22_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9168574.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="IWI22_001172"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="IWI22_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9168575.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="IWI22_001173"
     CDS             complement(29103..29843)
                     /locus_tag="IWI22_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9168576.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="IWI22_001174"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="IWI22_001174"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9168577.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="IWI22_001175"
     CDS             complement(31214..32152)
                     /locus_tag="IWI22_001175"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9168578.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="IWI22_001176"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="IWI22_001176"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9168579.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="IWI22_001177"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="IWI22_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9168580.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="IWI22_001178"
     CDS             complement(35312..35617)
                     /locus_tag="IWI22_001178"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9168581.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="IWI22_001179"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="IWI22_001179"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9168582.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9885 a         7988 c         6706 g        11988 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcgc ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 caaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc cagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcgattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 gtcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct tcttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagct aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgttcttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg atatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg cttaata
//
LOCUS       DACDNO010000022        35849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDNO010000022 DACDNO010000000
VERSION     DACDNO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.022"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(176..2347)
                     /gene="copB"
                     /locus_tag="IWI22_001180"
     CDS             complement(176..2347)
                     /gene="copB"
                     /locus_tag="IWI22_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9168583.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2367..4553)
                     /locus_tag="IWI22_001181"
     CDS             complement(2367..4553)
                     /locus_tag="IWI22_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9168584.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4553..4762)
                     /gene="copZ"
                     /locus_tag="IWI22_001182"
     CDS             complement(4553..4762)
                     /gene="copZ"
                     /locus_tag="IWI22_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9168585.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4775..5215)
                     /locus_tag="IWI22_001183"
     CDS             complement(4775..5215)
                     /locus_tag="IWI22_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9168586.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5289..5828)
                     /locus_tag="IWI22_001184"
     CDS             complement(5289..5828)
                     /locus_tag="IWI22_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP9168587.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6443..7909)
                     /locus_tag="IWI22_001185"
     CDS             complement(6443..7909)
                     /locus_tag="IWI22_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9168588.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(8220..10301)
                     /gene="cadA"
                     /locus_tag="IWI22_001186"
     CDS             complement(8220..10301)
                     /gene="cadA"
                     /locus_tag="IWI22_001186"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9168589.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(10825..11184)
                     /locus_tag="IWI22_001187"
     CDS             complement(10825..11184)
                     /locus_tag="IWI22_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9168590.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(11214..11555)
                     /locus_tag="IWI22_001188"
     CDS             complement(11214..11555)
                     /locus_tag="IWI22_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168591.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(11552..12226)
                     /locus_tag="IWI22_001189"
     CDS             complement(11552..12226)
                     /locus_tag="IWI22_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9168592.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(13279..14577)
                     /gene="asnS"
                     /locus_tag="IWI22_001190"
     CDS             complement(13279..14577)
                     /gene="asnS"
                     /locus_tag="IWI22_001190"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9168593.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(14617..15807)
                     /locus_tag="IWI22_001191"
     CDS             complement(14617..15807)
                     /locus_tag="IWI22_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9168594.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIETIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(15828..16355)
                     /locus_tag="IWI22_001192"
     CDS             complement(15828..16355)
                     /locus_tag="IWI22_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9168595.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(16402..19191)
                     /locus_tag="IWI22_001193"
     CDS             complement(16402..19191)
                     /locus_tag="IWI22_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9168596.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(19338..19538)
                     /locus_tag="IWI22_001194"
     CDS             complement(19338..19538)
                     /locus_tag="IWI22_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9168597.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(19990..20310)
                     /locus_tag="IWI22_001195"
                     /pseudo
     CDS             complement(19990..20310)
                     /locus_tag="IWI22_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20813..21931)
                     /locus_tag="IWI22_001196"
     CDS             complement(20813..21931)
                     /locus_tag="IWI22_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168598.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYYEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAIFRY"
     gene            22401..23708
                     /locus_tag="IWI22_001197"
     CDS             22401..23708
                     /locus_tag="IWI22_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9168599.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSESKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            23827..25025
                     /locus_tag="IWI22_001198"
                     /pseudo
     CDS             23827..25025
                     /locus_tag="IWI22_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25052..26320)
                     /locus_tag="IWI22_001199"
     CDS             complement(25052..26320)
                     /locus_tag="IWI22_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9168600.1"
                     /translation="MEKQLPSTVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(26483..27130)
                     /locus_tag="IWI22_001200"
     CDS             complement(26483..27130)
                     /locus_tag="IWI22_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9168601.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(27538..28041)
                     /locus_tag="IWI22_001201"
     CDS             complement(27538..28041)
                     /locus_tag="IWI22_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9168602.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(28164..28928)
                     /locus_tag="IWI22_001202"
     CDS             complement(28164..28928)
                     /locus_tag="IWI22_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9168603.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DVEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSK
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            29036..29311
                     /locus_tag="IWI22_001203"
     CDS             29036..29311
                     /locus_tag="IWI22_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9168604.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29907..30857
                     /gene="yidC"
                     /locus_tag="IWI22_001204"
     CDS             29907..30857
                     /gene="yidC"
                     /locus_tag="IWI22_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9168605.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(31036..32010)
                     /gene="lacD"
                     /locus_tag="IWI22_001205"
     CDS             complement(31036..32010)
                     /gene="lacD"
                     /locus_tag="IWI22_001205"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9168606.1"
                     /translation="MKKMSIEKQECLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDESVEINVQKQIVIERIDSECEAEGMPFF
                     LEILSYDATISDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVKGYG
                     QETCYSQAEASTYFYEQSKVTEQPFIFFSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(32007..32465)
                     /locus_tag="IWI22_001206"
     CDS             complement(32007..32465)
                     /locus_tag="IWI22_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9168607.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKEVYRK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKTEIATILTKEIGEDI"
     gene            complement(32462..33925)
                     /locus_tag="IWI22_001207"
     CDS             complement(32462..33925)
                     /locus_tag="IWI22_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9168608.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEGLEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTILADNEAVDTLESETISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMLKTVGDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     SGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVVFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPINKAAYVTGTALLAQGN
                     TTFMAGVSVACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDNKAANRTVIEEGRAGGLS"
     gene            complement(33907..34857)
                     /gene="pfkB"
                     /locus_tag="IWI22_001208"
     CDS             complement(33907..34857)
                     /gene="pfkB"
                     /locus_tag="IWI22_001208"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9168609.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQKKQIQTEFIEVPGMTRINVFTQVNQTREE
                     YKLVNQGPEIPQAAVHNFLNQIRALQAEDYLCVSGSLPRGMSPKVLVEISRICQEKEI
                     HLIIDSSDQEVMGCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENV
                     LLSLGEKGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLVGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            35000..35749
                     /locus_tag="IWI22_001209"
     CDS             35000..35749
                     /locus_tag="IWI22_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9168610.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFTDRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMREN"
BASE COUNT        10261 a         7703 c         6255 g        11630 t
ORIGIN      
        1 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaatc
       61 aattaatcaa agtaagataa caaaaaatct gagccagaat gaggttcatt ctgacccaga
      121 tttgattttg tacaacccat cagtccttat tgacaaagag cccctttttt cattattatt
      181 tgattttcaa tgtcaaagca tttagagcta caactaccgt acttaacgac atcaagacag
      241 caccaactgc tggacttaat atgataccaa ttggcgcaag aattccggct gctagcggaa
      301 ttgcaagaat attgtaacct gccccccacc agaggttctg gatcattttc tttcgcgttt
      361 gcttggctag atcaagaaaa tgcaaaatat cttttggatc gctgtttgtc aatacgacat
      421 ccgcagaatc gattgcgata tctgtccctg caccgattgc catcccgatt gaagcacggg
      481 ctaaacttgg cgcatcattg atcccatcac ccaccatgat tacttttttc ccttggtcaa
      541 ggtatttctg gacaacagct tctttatcat ctggtaataa accgccataa tattcttcga
      601 tccctaaata atctgctact gcttgggcag cgtttttatt gtcaccagta agcatgacag
      661 gagtgatttt tctttctttc aatgttcgga taaattcttt tgcttctgtt tttactacgt
      721 cacctaaagc aacgattgca gctagatgat tttctaaaat caggaagctg atggtatttc
      781 cttgttcttg gtaaggtttc aataatgctt gttctacgtt taatccgaga cgctcaactt
      841 ctttttcatt aactattttc acttgttgtt ttttgactgt tgcctctagc ccgactcctg
      901 aaagattttt tagattttca gcttgataag gcttgatttc atgttctttc aaataattca
      961 taatgccaac agccaatgga tgattggcat tcgcttccaa ggctcctaga tattgaagga
     1021 tttcattttt attgaacgca tcatcaagta cttcgacacc cgttacagta aattttcctt
     1081 ctgttagtgt tcctgttttg tccagcatga tcacatctag atcattggct tgctccagtg
     1141 cattgcggtt tttcaaaagt aatccgtttt tagctgctat tgatgtggag cgagcaacaa
     1201 ctaatgggat agctaaacct agagcatgag gacaggcgat gataaagact gttaccatac
     1261 gttctaatgc attaggaaga tctgtcaaga acaaccaggc gatgaatgcc aggataccca
     1321 caattaatgc gacataaaac agccattttg ccactttgtc agataatgac tctagcttgg
     1381 atttttctcc ttgtgctttt cgcaccattt ccataacctt cgctagataa ccattttcac
     1441 cagttccagt tacttcgatt tcgatcgttc catccccatt gatagaaccg ccaatcactt
     1501 tgtcattggc ttgttttttg attcctcttg attcgccagt cacagctgat tcgtcaacaa
     1561 ttgtttcacc tttcaagatt acaccatctg ttggcatttt atcaccagac cggacgatca
     1621 aacgatcacc ttcgtttact tctttcaatg aaactgtctc ttcttttcca tctttcgtca
     1681 agcgtttcac tgattctggc agtaattctg ctagtttttg taaagcattg ctggcattcg
     1741 acacagcatt catttcaatc cagtgaccta gtagcataat aacgatcaaa gtagccaatt
     1801 cccagaagaa atccatgacg tgtgtatgtg gattgagcag atttgcgata aacgaataga
     1861 cactataaat atatgcaact gtaatcccca tcgcgatcag tgtcatcata gcaggacttt
     1921 tttgtttcaa ttccatctta gctccactta aaaatggctg accgccatag ataaatagta
     1981 tagttgctaa gatcaatacg agccaatcag aaccaggaaa cgttacttga aatggaaatt
     2041 ccattcccat cattggtgaa aaaagaatga ttgggatagc taggataagc gatagccaaa
     2101 atttttgttt gaaattcccc atgtgcatgg aatgatccat gccgccgtgt tcgtgatcca
     2161 tgtgcatcga atggtccatt tgtttgctgt ttgctatatc tccatggtta tgtcccatgt
     2221 gcatggaatg atccatctga ctatggtcat gtttcatctc catctgtttt tcatgagatt
     2281 ctgtactttc tttgtttact acttcttttt tatgctcatg ttttttctcc atatcgttcc
     2341 cattcatttt tactccatcc cttctcttac tttttcatgt gtcgatttaa gctcagggag
     2401 ttcaacaata cactgacgga actaaacgcc atcgctcctc ctgcaatgat tggattcaaa
     2461 aagcctagtg cggcaaaagg aataccaatc gtattataga caaaagccca gaaaagattt
     2521 tgtttgattt ttctcaatgt caacctagac aactcgattg tttgtgcaat ggatgccaaa
     2581 ctgctattca tcagcgtcac atcggctgtt tccatcgcaa tgtccgttcc acttcccatt
     2641 gccataccga catcagctaa tgctaaggcc ggtgcatcat tgattccatc gcctgccata
     2701 ccgacttttt tgccatcttt ctgaagtttt tctacgtagg ctgctttatc ctctggaagt
     2761 acttcagcaa agatatgctt tggatcaata ccaacttgtt taccaatcgt ttcggctgcc
     2821 agtttgttgt ctcctgtcag cataaaaaca tctaatcctt tattctggag ctgcttgatt
     2881 gcttgtttgg cttctagttt gatttgatca gccacggcaa tccacccgat cacttgcttt
     2941 tcatcagcta aaaacatgac tgtttttccc tgctgttcta attctaacgc gtgttcttgg
     3001 tattcatcaa atgataggtt caattcaatt aatcgtttac gtgtacccgc aaaatagcgt
     3061 actccattaa tcgtaccgct gatcccagca cctgggtggg ctgtaaaatc aatcatatcg
     3121 tatgttgctg cttcttgcag ttttccgtat tccacgattg ctttacctag tggatgttca
     3181 gaagcttgtt ctaaagtata aaaaatagat aggacttctt ttgaaccgac tagatctgta
     3241 actttgggct ttccttctgt aatcgttcca gttttatcca aaacaatgct gtctaagtga
     3301 gcagctgctt ctaatgcttc tccgccttta attaaaattc catttcgtgc accaacaccc
     3361 gttcctacca taatagctgt tggggtcgct aaacctaatg cacatggaca cgcaatgact
     3421 aatacagaga cactatggat caatgcgagt tgccaatcac cagtgatcaa acctgtagct
     3481 atgagcgtga taaacgcgat gactaatacg atcggaacaa aaatccctga gatccgatct
     3541 gcgatttttt ggattggggc tttgctccct tgagcatctt ccaccatttg aatgatttgc
     3601 gctaatactg tctgcttgcc gacttgtgtg acttccatgt gtagcagacc attcgtattg
     3661 attgtcccgc caaaaagcgt gtcatccgga ttcttttcta caggaagatt ttcaccagta
     3721 agcatacttt catctatcgc actgcttcct gaaataattc gaccatctgc tggaatctgc
     3781 tcaccaggac ggatgactag ttgatcacca acgactactt cttcaatcgc taacgtttct
     3841 tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa gagacatcaa ttgtttgatt
     3901 gcattacctg ttttcgtttt tgctgtatgt tccaaatatt tgccgagcaa aatcagcgtg
     3961 atgatcatac tgctgctttc aaaataaaga tcttgggggt gtccacggaa aaatccgttg
     4021 taaatgctca atgcaaaggc agccgatgtt ccaattgcta ccaatacatc catatttggt
     4081 gctttcgtct tgattgcatg gtacgcacct ttataaaaac gagcaccgac ataaaattgg
     4141 acaggagcag acagcactaa ctggacgatt ggaaaatgaa aaaagtgaac aatcgccgca
     4201 tggcttccta acatcatagc aatcattgac aacatcaacg gcaaagtcaa aaccgtactc
     4261 aatataagat cgaatagcat ttttttcaaa tacgcttgct tttcttctgc aattttttgt
     4321 ttgtgggctt catcgtacaa aattgcacca taaccgatgt tttctacgct ttggattaat
     4381 ttttcagtgg tcgtcccttc atatttgaca cttgcttttt cagtggccaa gttgactgtg
     4441 gcattgacca caccaggttg tccgttcagc tctttttcga tcctagcaga acaatttgca
     4501 caagtcatac ctgtaatgac gaacgtatcc attttggttt ctgctgttgc catcaaatca
     4561 cctctgcttt ataacccaat ccattgattg cttgacaaat cttttcggat tgaacttttg
     4621 tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt tactttcact ttttgcacgc
     4681 cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca atgattgcag ctcatacctt
     4741 cgattgaaaa ttgttgtttc attttttctt cctcctattg atgttgttta cattcacatt
     4801 gacctgggat acagttacat tcgatcgtat ctactggttg cttttgttcc agttgtttca
     4861 aaatctcatt gatatcttct tgagtcaaag tggcttcttc tactagatcc gcaatcgtct
     4921 ctccaatcct ttttgcacag atatgggaaa aaagattttc cgtcgcactg cgaactgttt
     4981 ccatctccga gacagctgga tgataaatga atttttttcc ttcttgttct gtccagatca
     5041 cttctttttt taccaaacgt cccagcaaag ttttgatcgt tgctggtttc caatccatcg
     5101 aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg tccaagtgtc caaataacac
     5161 gcatgatctc ccactctgaa tcactgatct ttactggcgt tttgactttt tccatttatt
     5221 tcacaccttt cgtttacaaa tgtaatcatt agcttatgaa tacagattac gcttgtaaac
     5281 aaaaactgtc aattattttt gctctctatt ttcaatttag ttaaaaattc ttgtttctct
     5341 cgtcttgttt taaggatgat cagtttattt cttgcattag ctacttggat cagctgctgt
     5401 attttcggtt cattgttttc ttcaaaatgc cagataaaag ataaaaattc taagtattcc
     5461 ctgtttagtt tctctgaaaa tgattcaggc atatctggcc gagatttttt gtttatcttt
     5521 ctttttagag aacgccaaac tactcggtag atggcgagat aacgcggcac tttaagccaa
     5581 atgatcttat ctgcttcttt aatcctttcc tctaatgtag aagtataatt tccttcaaca
     5641 atccatgacg gattagatac cataaacgat tgctgttctt gcaaaaacca cttttttgcc
     5701 tgaatactgt agtctgttgt atgccataaa aggtctagcg gcagtagcgg aaagtcggtg
     5761 atttctttga tcttccttgc gagggtcgat tttcccgacc cactttgtcc aatcacgatg
     5821 atcttcattc ttctccctca cttctctttg gaatttggtt cgatcatacc acttataacg
     5881 ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaataaat
     5941 ggtgttcaat cagctgacct tcagcattaa ctcaaaaaac ggcaaaatat gaggagctat
     6001 tttttcgttt gttcctctaa tactcaggtc aaatcgctga ttttacaacc tcttccttaa
     6061 acggtgttct cctgccaaca cctcggtcac aaggcacaat agtcaacaaa catgagaagt
     6121 tgttttttga aattgctcct tgtgactcgg agttaaacgg caggctcact acctctttct
     6181 taaacggtgt tcaaaagctt gcatctgcgg taataagccc agcaaaacga aaaatatgag
     6241 gggctatttt tgtttggctg ttcttattac ttgatgcagg acagcttttt cacaacctct
     6301 gattcacggt gttccagaag tagcttctgc gaaaaacaag ccgaaacttc acaaaaattt
     6361 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     6421 tcttgtaaca agtatttcta ttttaaggct gaaccactgt ttctggttca gtgatttctt
     6481 tgtttttgtc tcgacgagca attgctggca agatcatacc taataagatc aagacaattg
     6541 gtgtgatgat attcgtgaat aattggaacc accatgcttt aggatcagca gtataatcta
     6601 cttttggcac cattcctagg atacatgcaa acgcagtgaa ggcaaagcac caagcaccaa
     6661 agataaaacc gatttttgga tttttaacaa atttatattc agaattgaat ttcttataag
     6721 ctttattcaa catcatatat gccaagaaca cccataggta acgcattggc atgacaactg
     6781 agttcaagtt cgtcaaccat tttactaatt cattcatatc tttgatacca aagatcggca
     6841 acaaaataat gattcctact agtatacctg tcagaagata accattcacc aatgtacctt
     6901 tacgattacg tttacgtagc cagtttggta caaattctgg atccgcatct gctaacaaga
     6961 tttttagtgg tgcatcaatt gaaaaggcta atgcggagat ttgtccaatc atgtttgtca
     7021 atgcgtaaat gatcatcaat gagttaccga taccatagta attacctaaa atttgaaaag
     7081 cactgtatgc cccatttgcc atcaagtctt taggaatatt atcgctagca aacagcatac
     7141 ccattgcgac tgagcctaat acagcacaaa tccctaccat tgctgctaag aagatcatcc
     7201 ctttaggaaa ttcctttgca ggatttcttg tagagttgac ataaggggat attttttccg
     7261 ctcctccaac tgcaaatact agcatcgaaa cggttgtgaa ataactgaaa tcaaatgttg
     7321 gaatatatgt tttcacttga ttcatatgtg gtgtggcaaa ttccatcgtt ggattgatga
     7381 acggtgccgt tacagctaaa agaataaata agatcgacat gacgaacatc gctgttccgg
     7441 ccagtccgcc aatcactttc aaagtagaaa gtccttttgt tgataaatat aaaaaggcaa
     7501 gaaatataat aagtgaaata actgctacca tttgtactga catcgtattt acgatatttc
     7561 catttccttg gactgcccaa ccgccagcaa tcaggattgc ttgaggcttt tgtgccaaat
     7621 aaggaatatg gacgacccag taagtccaag cggcatagta tgccagtcgt ttattagatg
     7681 tcgtattttc gacccatgaa ctgacgcctc ctttactatc cttgaaggct gaccctaatt
     7741 gtccaacgat caatgcgtat ggaataaaat aaatggccaa aatcaatagc caagaggtga
     7801 caactgatac tccttgctga gcatagttat taactacatt ccccatgccc caaaccatgt
     7861 taaatgcaat taacgcaaca gtaaaccagc gtaattgttt gttgttcatt cgttctctcc
     7921 tttatgataa aaatttgtta tccagatgta atcatattca atttttaaca aacttacaag
     7981 acagtgttat tttatgtgtg tattctttta tttaatacac atcgtttttt catatttttt
     8041 ccatcaaaaa aataagttat tttcttttat ttcatttacc ttattgaatt tcaagctatt
     8101 ttgaattttc tgcaaaatta aaaacattag ttattttata atcctatcta ttttctttac
     8161 atttttcatt aagaaaagga tataaaaaaa agaggacgtt cattcgtcct cttcattgat
     8221 caacgtttat tttttacatg caagcttcgc atggcattca aaacagctaa tactgtcacc
     8281 ccaacatctg caaatactgc atctcccatt gatgcaaaac caaaagcacc taaggcaagt
     8341 accaaaattt ttatcccaat agcaaagaca atgttctgtt tgacgatttt caatgttttc
     8401 tttgctaaat ggattgcttc tgcaatcttc gatggttgat cattcatgat gactacatct
     8461 gctgcttcaa ttgcagcatc gcttcccaat ccacccattg cgattcctac atctgctctt
     8521 gctagcaccg gtgcatcgtt gattccatct ccgacaaatg cggtcttttt attgtttctt
     8581 tgtaagattt cctctaaccg ctgcacttta tcttctggaa gcaattcgct atatatcttg
     8641 tccacgccta cttgcttccc gatatgatca gcaatttttt tcgaatcgcc cgtcaacatg
     8701 actgtgttct tcacaccagc cttttttaat tgaaccaatg cgtctacggc atcttctttc
     8761 aaggtatctg caataacaag ataaccactg taactttgat cgatcgctag ataaagcaat
     8821 gtaccgattt cttgacttga aggaaaagag atttcaaatc tttccatcaa tttgtgatta
     8881 cctgctaata cttgtttgcc atcaacttca gctaagatcc cttcacctgc aaattcttgg
     8941 atattagctg ttggtaaaag cggacctgcg tagccttcta aaacagattg agcaatcgga
     9001 tgtgtagaaa actgttctgc actcgcaaca tattgcatga atatctttgg atcgattttt
     9061 gtatcgatcg tctggatcga aaagtcacct ttcgttaatg tgccggtttt atcgaatacc
     9121 actgtctcag tttgtgcaag caactccaag taatttgaac cttttactaa tacaccgatt
     9181 ttacttgctc ctccgatacc gccaaagaaa ctaaggggta cggagatcac taatgcacat
     9241 ggacatgaga tcactaaaaa tgtcaatgct cgatagatcc attcagaaaa cgccgctcca
     9301 gaaacaacaa gtggaggtaa aacagctaat aaaacggcca atcctacaac gactggtgtg
     9361 taatagcgcg cgaatttagt aatgaaattt tcagctggtg cttttttact gctggcattt
     9421 tctactaatt ctaataattt gctgatagta gaattttcat aagatttttc tacttgaact
     9481 aaaaatggtt tatttttatt gataaatcca cttaatactg tttcaccaac atatacagat
     9541 ttagggactg attctcctgt aagtgctgac gtatcgacta atgattctcc ttcgatgatc
     9601 gttccatcta acggtacacg ttcaccaggc ttcacaagta caaactgacc gatctgcacg
     9661 tcttcagggt tcacggtcat caatccttcc gtcgtctgca cgtgagctgt ttctggacgg
     9721 atcgccatca attcgcgaat cgattttctt gaatgactga ctgccaatcc ttggaaatac
     9781 tcgccgattt gataaaatag catcactgct acggcttctg gatattcacc gatcaacata
     9841 gcgccaatcg tagcaacaga cataagaaaa ttctcatcaa agatttttcc tttgacgata
     9901 ttcatcactg ccttcttcac tacatcatat cctatcagca gataaatcaa taaataccct
     9961 atcaagcgaa ttggttcatt aaaatcaaat aatagcaata aagcgatacc tgcaagggaa
    10021 aataaaatcc ggaacagcgt atttctctct ttttcatttc catgttcaga tactttttcc
    10081 ttatctattt ctgttacttt gacatctggt tctaattcct tcactacttt ttttatttct
    10141 tgttcaatcg tagccaaatg atcttcctct gcgtcaatcg ttagttttcc agaagtgaag
    10201 tttaccgttg cttccttgac tccattgatc ttctgaactc ctttttcgat tttcattgca
    10261 cagttcgcac aatctaaacc ttctaatctg taacttttca ttttaatgac ctcctcgcct
    10321 caacatatga atatctcttc ataactaatt ataaatgaac ggttattcat atgtcaaggt
    10381 atccgaaaga aaaaatgaaa aacatctgca tcggaaaatt tcttccatac agatgttttg
    10441 gcttattctt tctttttctt ttcgatcatt gccactgctc ttatccaacc cgcacttgcc
    10501 ttcttgatct tgacaggata gcaactaagt ttgaagcctg ttgctggcaa tttgtctaaa
    10561 ttcattaatt tttcgatagg acaatatctt ttttcaatcc ctgcaaaatg tccttcccag
    10621 atattcgaag ctccactacg ctttcaaaaa tatagaaaga gttctttaat gaaaattatt
    10681 tcaactttcc ataaacagta accccatata aataaaagac gtaaaagtca catggaagaa
    10741 cttatacagg agaacgcata gagtcaggtg aaatacatgt atcccacctg actctatgct
    10801 ttatctaacc tttattattc tcttttatac aatttctgca ccgaatggca tcaacgccaa
    10861 tcgagcaagt ttgaaagatt gcttggcaaa aggtatccct atgatcgtga tacatagaag
    10921 gatgccgctg acgacatgtg cgagagcaat tggtaggcct gaaacgatca gccagatgat
    10981 attaacaatc aatgacatgc cagactggtg ataaattact tctttgccaa aaggcgcaaa
    11041 agaaagggag gcgatcttaa aacattgcat accgattggg atacctatga tcgtgataca
    11101 ccaaagtatc cccgctgcta accagctaag accaccgatt agtccgccga atataaacca
    11161 gatgacattt cctaaacaac tcattgtatt tctccttcta ttactgtttt tgattaaaac
    11221 tgggcaaaaa atgattttat tcttcttttt ttagcttcca gtatcaaata ttctgcaaat
    11281 tttttttgga tttcttccgt attttctttt gttactaatg tgtgttggat tagttttcct
    11341 gatggtcgct gatccaatcg ttcttctgac tgttgcacgt ccgcttgcgc aaatccgata
    11401 atccgttttt cttttgttac agcaacgagc aatagtttat tcttatcatt taggtcagct
    11461 gccgtttttg cttttacgaa tgattctgga tgatcagtaa tcagctgatt gatttgaggt
    11521 agatcttctt ttactgccca ccgtatttcc atcatttctc acttttcttt cgctcatttt
    11581 tatctccact aaaaaaatcg actgcggcaa ttgcatctat ccagctttgt gcaatgatct
    11641 catgtccgcc aatcgttggg tggaccccat cttctcttgt atatttcgaa aaaccataaa
    11701 caatccctaa agcattgaat ataccatcta aaggaaccag caatgcgcca tattccttag
    11761 caagtttacg aacaatttgg attttagggt ctaagtcttt acgccagcca tgacggtcgt
    11821 cggttatagg aagtacaaac ggctccatca agatgatgtt gcagattccc actgtttgaa
    11881 gttcatttag taattttcga taaacggatt caaattgctg agcagatttc tcagttccga
    11941 atatagcctg atcgctagta ttatgccagg tatcattgat tccgatcaaa atcgatacat
    12001 agtcaggttg taaagctaaa caatcttctt gccagcgtac ttgcagatct gcaattttgt
    12061 ctcctcctat cccacggttc atgcattcaa attccatcgt tggatattgg gagattagtt
    12121 ttccggcaat caaaagtgga taacctttac ctagatcagt tgcttctgct cggtctcttc
    12181 ctgcgtctgt aatgctatca ccgataaata atatacgttt tttcatttgt caaaaactcc
    12241 tttttgaata aaacgatgat tttcttcctt atattatccc ataagcccta tcaagacgaa
    12301 agagaaaata tatgtataaa aaatgacacc agcctagtcc cttcaaacaa ttgaatgaac
    12361 gctcatcaag tgtctagttt tttatgctat ttttttctta acgtgccgga ttccccttga
    12421 ttccttcgtt gtttggtcca gagagatcga tcacacctaa gcttccgtca atcagaccac
    12481 tgatgacgcc atcagaacat atatttatag gctcatctaa gacttctagt aaatgtccct
    12541 ttactaatat ctcttctaga ttgcttggga tagattggtc ataactttat ctggtccgat
    12601 gatttcaagg gtgaattgaa cttacatatg gattattgca tgctgaaaat acggacctac
    12661 tccatcttcg aatgttacta ttagtttgtt ttcaggtgct agatgctgct ttatttcaac
    12721 agcagaagca tcatcaaatc agatttccat ttgaattacc ttctttgtaa atgtttgatt
    12781 gattgcgatt tcgttataga accttgtgaa tcaaatagca atttcttttg ctttcttctc
    12841 ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa agaactgcga gagttcgcag
    12901 ttctttttta gaataaacgg tgctctccgg ccaactcctc ggtcacaagt cacaatagtc
    12961 atcaaacatg aaaaactgtt ttttgaaatt actccttgtg actcggagct gaacggcagg
    13021 cgcacaacct cttccttaaa cggtgttcaa aagccagttt ctcggtatta agccaaattt
    13081 atgcaaaaat gtggaaagcc atttccacaa aatttttctt aatactcgaa actaaacagc
    13141 tttttcacaa cctctccact aaacggtatt caaaagctta catctgcggt aataagtccc
    13201 gccaattcaa aaacatgaac agctgttttt gcttggctgt tctaattact tgatgcagaa
    13261 cagctttttc atcacctctt atggataaat tcggttcaac aaacgtggga atggaatagc
    13321 ttcacgtaca tggtcgatac cagcaatcca agtgactgtt cgttcaagac ctagaccaaa
    13381 accagagtgc ggaacagaac cgtatttacg tagatcaaga taccatgaat attctttttc
    13441 atccaaaccg tgattacgga tttgttctag caagaaatca tagtctgttg cccgttcgct
    13501 tccgccaata atttcaccgt aaccttctgg tgcgatcata tctgcacaga tcaccacgtc
    13561 ttctcttgtt ggatgtggtt tcatgtagaa tggcttgatg gcttttggat agtttaggat
    13621 aaagacaggc tgttcgaatg aattcgcaat gaacgtttca tgaggtgaac caaagtcttc
    13681 gccccattcg atgtcatcaa atccattttt cttcagtaat tcgatcgcat catcataagt
    13741 aatacgaggg aatggcaatt ttgtataagt ttttaagact tctttgtctc gtcctagtac
    13801 atctaatgca tattcgcaat gatctaaaac attttgtact aagaatgcta catattgttc
    13861 ctgtacttct aaactttctt cttgatgagt gaaagccatt tctggttcga tcatccagaa
    13921 ttcgatcaaa tgacgtcgtg tttttgattt ttcagcacgg aaagtcggac cgaatgaaaa
    13981 gacttttccg aaagccatag cagcagcttc catgtataat tggccacttt gagataaata
    14041 agctttttga tcaaagtaat ttgtttcgaa tagatccgta gtgccttctg gtgcagaacc
    14101 agtcaagatc ggcggatcaa ttttgacaaa atgatgttga ttaaagaatt catacgttgc
    14161 acgaatgatt tcatttctga tctgcataat cgcatgttgt cttgaagaac gcagccataa
    14221 atgacgatga tccatcaaga agtctgttcc gtgttctttt ggtgtgatcg gataatcgtg
    14281 gctctcgctt accacttcca catttgtaat tccgatttca taaccaaatt ttgaacgact
    14341 gtcttcacgg atttctccag ttactaacac ggatgtttct tggtgtagat ttttagctgt
    14401 ttggaaaact tcttccgaca cttcgctttt tacaactaca ccttggaaaa aggctgttcc
    14461 gtctcttaat tgtaaaaatg cgatttttcc gcttgatcgc ttatttgcga cccaagcgcc
    14521 gattgttact gtttctccca catggttttt cgaatcgatg atttgaattt gttccacgta
    14581 tgactctcct tttatattcc ttgaatttct ttaggattaa ttttgacttt tactctcaat
    14641 aaattgcttg attctgcgta ccgcttcttc taacgtagcg cgatcagtag cataactcat
    14701 gcggacattt tctggtgccc caaagccttc gccagtcaca agcgctaccc ctgtttcttc
    14761 taaaagatct tctacccatt gtgtgacatt ttcatattta cacatgcgta gcgtttcttt
    14821 tacattagga aaaagataaa aagctccttg tggcttttca agagaaacac ccggcagttc
    14881 tgccacaagc gaatataacg tattcaggcg ttcttcaaag gcgttccgca tttcttccac
    14941 tgtatcttgc tcgcctttca atgcttcaac agctgcatat tgactgactg ctgtcggatt
    15001 actggttgac tgggaagcaa cagcgatcat tccattgatg atcacaggat ttcccaccgc
    15061 ataaccaatg cgccagcctg tcatcgcata tgttttagac acgccgttga tgatgatagt
    15121 ctgatttttg attgcttctg aaatcgttgc aattggagta aactcattgc cattataaac
    15181 taaacgacca taaatatcat cagcaactat caaaatatca ttttttactg cccattcacc
    15241 gattgcctgc agttcattct tgctataaat cattccagtc ggattcgatg gagaattgat
    15301 gattacggct tttgtttttt ctgaacgtgc ttgttctagc tgttcaacag taactttcca
    15361 gctattggat tcttcccctt tcacaaagac aggaagtcct tctgccagtt ttacttgctc
    15421 accgtaactg acccagtaag gcacaggaat gatgacttca tcttgcgggt caaggatcgt
    15481 ttggaaaagg gtatacaaag caaatttcgc tccgtctgtt acgactgttt gtgaaggttc
    15541 atagacaagt ccatagtttt tcttcagata atcaacgatt gcctgtcgaa gctctgggat
    15601 tcctgctgtt ggtgtataat agctagcttt cccattgcgg attgtttcga ttgctgcttc
    15661 ttggatgttt tctggtgtcg caaaatccgg ttctcccaca gtcaaactca gcacatctct
    15721 gccttttgct tttaatgcct tggcctttgc cgcagctgcc aaagtgacag acggttctaa
    15781 tcgttttgtt ctttttgata actccatccc attcattcct ttacctttta aattcccttg
    15841 attgtcttga cttcatctcc agattcaaag ccaatcaggt aataattcag tttcccattt
    15901 gtttcttttc ctactacttc ccacacaggc tgatcatcat acatccctaa agcagctttt
    15961 tcgatttgga tttcaggatg ggccgcagca attgtctgtt ttgcttcgtt ttcagtcagt
    16021 ccctttgttt gatccaatac ttttactttt tcaccagatt taggaatgat aacggcgatt
    16081 tcttttcctt tatcatttgt acctgtcagt gaaaagtatg tcttctttcg agtaaaccaa
    16141 taaaaatgat cggtttcctc aagatttgca tatttctttg cgatttctac tgcttctttc
    16201 ttcgcctgca ccgccggacg actggaacgc aagtaaaaaa tcgagctgat cacgatgacc
    16261 gctaataaaa atacgatcaa gccaagtaac acgcgattaa tcgttttttc ctttgtctgt
    16321 tttttcttca agggtcgtcg ctcctttatc ttcattcctt ttcattatag cagattttaa
    16381 ttatgaaaaa agacagctcc tctaacttcc ttcttcattt ttctttaaga attcatggat
    16441 atcctgatag atttcctcca tagctagctc tttgatcggt acttttttcg gtaatgcatg
    16501 gatgatccgt gcgccatatt ttgcagtgac tagtcttcga tccaaaataa gcaatacgcc
    16561 acgatctgtc tccgtcctta tcaagcgacc taatgcttgg cgtaacttca aagctgcttt
    16621 aggtacggat tcctggtaaa aaggattgat cccctctgat tctaaataag cgttcctagc
    16681 tttgaccatc gggcgaagcg gattatcgaa gggtaaacgt gtgactacaa cgatctgcaa
    16741 agcatctcca ggcaaatcta caccttccca aaagctgtca gcgccaaaca atacgctttt
    16801 ttctgacaaa atgaagcgtt tcaaaagctt ctcccggctt ccaccaaacc cttgagctag
    16861 tatttctcgt cctttttcta gcatagacag atgcattttc tgatagacct tctgcaaaac
    16921 atcatgagaa gtgaataaca caagtattgg ccgatcttct tgttcgatca gacctgtaag
    16981 tgctgtctct aaataatcga tatatgcttg attgctgact tgatggacgg caggtgcctc
    17041 ttttggcaca tagatccttg cctgtttctc ataatcataa ggagaggcaa tcactttaag
    17101 aggtgctgct tcaatgccaa gcctcttaga gagatagtgt cgattagggc cgatcttcag
    17161 cgttcctcca atataaagaa tacgatcata tcgaggatac caaacagtcg acggcaacat
    17221 agccgcttga aaatcatgga gatgaaaaat catctgctgc gctgtgttcc caggaacgaa
    17281 ccaatggatc aaatgatctc gccagtcttc cagccattga tccatcagca agtattgctt
    17341 gtctaattga tcaaataggc tatataaact tgctaaatcg atccgcatct ggtttgtcca
    17401 gctttcttgg ataatctcaa aagtcagacg catttgtgac tgtagctgct ggatctcctg
    17461 ataaaaaagc tgcaggtgat caatggctgt cccagattcc agtggcagct gatccaacat
    17521 ctcttttgtc atcatgactt cttctggata ctgtccgtta ggttgcttgc cgctcactaa
    17581 actcattaat ccttctgcca tacaaatctg tgcttcgcat agcgcagaaa gttcttctcg
    17641 ataaatcgat aaccagcgta aggcttcatg ttctttcggt aaatagcttt gtattcggtc
    17701 aaacagctga tttggttctt ctaattgctg gatttgttta tggaaatagt taatacttaa
    17761 tcgtttgtca tttacttttt cagcgatttc cggcaaatga tgtacttcat cgatcaacaa
    17821 aaatggacta gctggaagca atggttcttt tcgctgtgtt tcttgtacta aaaacgcatg
    17881 attgacaatc aaaaaattac tttgcctgat tttcttttgc aagaaacgaa caaagtcttg
    17941 ttcgtaaaat gactgatttc ttgctaaaaa tccaatacct ctgtgcgcga tatctgcaaa
    18001 gaatgaatgg ttcaaatttg ttaaatgtaa ttcatccaaa tcaccagtct tcgtttgcgt
    18061 cagccaaact aagaccgcca tttgatacaa agcatattgt ttttgttctg gcggttccac
    18121 aagtgtgcct ttaaaacgct gcaaatcaat atagtggcga tagcttttga taattacagg
    18181 ttgtatgggc tgatctagca attgattcag caatggaatg tctttttcta tgatctgctg
    18241 ctgcaaaact agcgaaacgg tactgatgac agcgggtttt tcaggtgtag ccaaataact
    18301 caatggcagc agataaccga ttgttttacc cattcctgta gctgcttcga tcatcaaatc
    18361 ttttgattca tcttttgtga aatggtcgta gaccgcattc atcagtctgt tctgttcttt
    18421 tcgatacatc agtgttttac caaacatttt ttcttttgct tgtttttttc ttggatatgt
    18481 ccgctcgcca taatggacgg atgtgaatag ttccacttct tttttctgca aagctagccc
    18541 atcgacgatt tctaatgagg gatcaagtgg ctctggtttt tctttcattt cttcaatcac
    18601 atgataaatg aaacgactcg tatccatacc tgtgtgtcta cttaatcgcg caatctgctc
    18661 taacgtgatg attggcaatt ttctcatccg ttcttcgatc agcaaaagaa gttgacctgt
    18721 cacttcggca tcactatctg cctgatgagg attctcatga ataaatccaa ggctttctga
    18781 caaatcagct aagcgaaagc ttggttccgt cggcaagaaa atttgtgcaa gttctactgt
    18841 atcgatgcct ggtatcttca aactaggaag tccgcatcgc atgagttcat ggttcaaaaa
    18901 attataatca aaataaatat tatgcgcgac aaatatggtg tctgccaaaa gattgtaaat
    18961 cgtttgggcg acatcttcga aataaggcgc tttttggacg cgttgattcg tgatccctgt
    19021 taaatgctgg atttgttttg aaatcttccg ccctggatga atatcgatcg aaaaacgtga
    19081 agtgatccga cctgattcaa tcatgacgca gccaaattgg atgatccgat cttcttttgg
    19141 attcgtccca gtcgtttcga tatcaacgac tgcataagtt cttggtttca aaatatcttc
    19201 ctcttctcct gcctctaatg ttcttttgaa attatactac aaagtgcaaa aaaatactct
    19261 catcgctttt taaagaaaat agacaaaaac gcccagacac gcaaatgtct gggcgtttat
    19321 tttttagtat aggttaatta agctttagta acgtttgttg cttgaggtcc gcgttggcct
    19381 tcttcaacat cgaaagttac tgcttggcct tcttctaaag ttttgaatcc gtcgccttgg
    19441 atagctgaga aatgaacgaa tacatcgttt ccgttttctg cagtgatgaa tccaaatcct
    19501 ttttctgagt taaaccattt tactgtacct gtttccataa taaaaatcct ccttgtgtat
    19561 caacacatat tttgcaatta cttgaatggt gtatatctgg aagatgttta tttgaaacaa
    19621 gctaccacag attaccgaac aaaaactact cctatagtat atcataccgt ttacaaaaaa
    19681 gctagagttt ttcgactttt tttattaaaa aaagttttct cagcgattac ataaagtgaa
    19741 gaaccgaact ttttttaaaa taaacgacat attgttcagt tggtgttttt ttgctctgat
    19801 gttcttcttt aaactaaaaa aattattctt tctgactact tatcggttct gtttcaacgg
    19861 tcgaggctgg ggaagattct ttagtaggtc tgatgttctc atggtatttc tgatagccta
    19921 tgacagtgac cagaagaaca aggcaaacca atacgatcaa cgagattagt ttttcttttt
    19981 tgctcatatc tatacctcat aaataacagg atgatgtcca tcatatttct gagtatgagt
    20041 aaaagcaaaa gctgcagctg tagaatgtaa gaaatgccag caactttctt aatcacaggt
    20101 cggacagtac caaaagtaac caaatcttgg ctgcaaagac cgttcttctg tttcaggaat
    20161 cgcagaatca agatttggtg tctgtacata aacatttttg tacagacgtg tgcctgtaat
    20221 aggagaccgc tgtgtttctt cagcaaaaac tggctgactt gttaaactta gggatagaaa
    20281 cagcaaacac cataacagtc tttttttcat aaagacttcc ttatcgataa gtttttccca
    20341 attaaaatca tatctcaaga acgcccgtta ttcacatttt gttcataaat gatgtttttt
    20401 gtatcataat cgtcaaaaag aggtcgtgac cacgtcacaa cctctttttt aaacggtgtt
    20461 ccagaagtag cttcttcgga aataagccga aatttcacaa aaatttgaga aataattttt
    20521 gtcaatctct tcttatttct tgaagctgac cacttctgtc acaacctctt ccttaaacgg
    20581 tgttcaaaag cttgcatctg cggtaataag cccaccaaac aaaaaatatg aagagctatt
    20641 tttgcttgct gttcttatta cttgatgcag aacagctttt tcacaacctc tcatacacgg
    20701 tgtttaatca gctgaccttc ggtattagct caaaaaacgg caaaatatgt gaaactattt
    20761 ttccgtttgt ttctctaata ctcaggtcaa aacactgatt tcacaacctc ttttaataac
    20821 gaaaaatcgc tgtcagactc ttttcatctg gcgcatcttt ttgatccaaa ataaacactt
    20881 ttccgccatt aatgatcaca tcatctgcta atgcattcaa cacttgacgc cgatcatatt
    20941 ctgtttcagg atcttcaccg aaaccatcag ctaagtttga cgtagcgata aataaatgtg
    21001 acacttttcc ttctttcgca gctggaacga tatcaactaa ctgatcgatg aatttcttat
    21061 ctaagagttt attgtattct tttgattctt gagctgcgag ttcacttgac agcttcttcg
    21121 ccccttcttc gatatctcta aaggatagct gcgcaggaga agcagaaatc gcaacgtctg
    21181 cacaataata gggattttta gcaatttttt tgaacatcgt ctgattttct ggtagagcaa
    21241 ataaataaat cggccattgt tctggatttt cgaaagaatc cttcaagtac gtatcaactg
    21301 cttggtaata gttgacccaa tcgatctcta cttcttcatc ttttggattt acgccatgga
    21361 aagttactcc atctcgggct ccgcctcctc cattagaaga gaagttcaaa ccgccacctg
    21421 tcagttcaga tcccaatgca gtaacgacat caaccggtgc cccttctggt agagatactt
    21481 cttttacttg tttgttgctg acttcataca atttcatgga gtcgcggttc aatcccaata
    21541 aataataaga atagttgaac tgcccatttt tgataatagc cagtaaataa ggaagatcac
    21601 ttacatagta ttgatcgtct accgggatac tcaaacgatg gatataggtt tggtcagcag
    21661 ttataatgac cgctacactc gtagttgcac tacgccaaaa cgaaggatct cctaacaaat
    21721 tttcaatttt ttcttggaac ggggaccagt catgttcgta atattttttc tcaaaacgtt
    21781 tctttgcttc tttcgcaaaa tttttgaatt tgatctgatc tttttccaca tcttgatggg
    21841 cgacatgcgt attcagcatg atcgtgacga atggtccttt agcgccttct gaagtcaatt
    21901 gtgacaataa ttctttatcc ttttttgcca taaacgtacc cctcctaaaa aaatcattcc
    21961 tttgtttcta tctaaagtct accacaacac tatttttcca tacaataaaa cgtatctaaa
    22021 aaaataaaac ggtttctacg gttttaaatt tagctatttt tttaagttat cttcataatt
    22081 tcctcaatta tttagcccca gtcttcaact attcttcatg aaatgctgat aaactaagaa
    22141 aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat taagtattat
    22201 cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc ctattcctgt
    22261 aaaagtacgt aaaaacgacc gctaaccttc cataacgtgc gttcttatcc taaagttttt
    22321 tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag cgaaacaaat
    22381 taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta ttattggcta
    22441 gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct gaagatacgt
    22501 tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag attctttatg
    22561 atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc ggtctttaca
    22621 tcgttcttga tcaagtaaaa gaaggaaaac tatcatggga tgataaagtt tcgatttcag
    22681 attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg cataaagaaa
    22741 acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc aatgcttcaa
    22801 tggtggcctt ggcagaaaaa atctctggca gcgaatctaa attcactgaa cgaatgaaag
    22861 aacaattgaa agattgggga atcaaagatg caacgatcgt taatgcatca ggactgaata
    22921 actcttatct tggcgaaaac cgaccagaag gtactggaga aaacgacgaa aatcaaatgt
    22981 ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggattttcca gaaatactgg
    23041 acgtatctag cactacgaca caaatgtttg gcgaaaacac gcaatcacca gtggaaatgg
    23101 tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg gacggattaa
    23161 aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact aaaaacgatc
    23221 aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg agtgcccgct
    23281 tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa aaagagctcg
    23341 gaaaagctaa cgcaagtatt cctagcctaa aaaccattga tgtaaaagac ggaaaagaat
    23401 caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac gatatggata
    23461 ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac aacgagatcc
    23521 aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg gcagacgata
    23581 aattaggcta tttagaagac aacaatgcgc catcaacaga gatcattacg gataaaacag
    23641 tggaaaaagc gaatatgttc gtgattggct ggcgacgcgt cgcagaattt ttcggcaact
    23701 tgttttaatt gaacaataag aaaagcatct ttccatcgga aagatgtttt tttgctttta
    23761 ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga aaaaggagtt
    23821 gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat tggggtattc
    23881 tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta cggcagtacg
    23941 tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta ttcgaccttt
    24001 tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt cacagaaaaa
    24061 cgaaggctgc tgaatggtct tttattcaat gttttcctca tcagtttcgg catctacttg
    24121 tttgtcttgc tgtatgaaac gcaaaatatt ttccttggcg gactgatcgc cttgatcacg
    24181 attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt tcttttttgg
    24241 aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt aacgttgata
    24301 ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa atatttgccg
    24361 caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct atttatcgta
    24421 ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg atacaatcaa
    24481 gacttcatcg ttgtgttagg cgctgggttg atcaatggag aaacagtttc tcctcttcta
    24541 gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc tacattaaat
    24601 ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc agaggccatt
    24661 gcgatgaaac aatatgccat ggaacaagga attcccgaaa gagacattct tgtcgaaacc
    24721 aattcgacaa ccactttgga gaacatgctc tattcaaagg aaatcatgga tcagcaaatg
    24781 aaagggccat accgcgcgat cttcagttcc aacaattacc acatttttag agccggtctt
    24841 tatgcgcgac aagccaaatt gaaagcaaat ggtattggtg caaaaacagc tttctactac
    24901 ttgcctaatg cgttttgaga gaatacatcg ccatcttgat gatgaacaaa aaaagacata
    24961 tgatcatttg cggattgctt ttcgcaggct ccgcattctt atcgctgatt actttacttt
    25021 tctaaaaaaa caaggtgtga caaaacatat gtcacacctt gtttttttct ttcattctct
    25081 taactattgg caaaaagagt atcccttttt tgaccgctac ttgctgagca gaataaatac
    25141 cgctgttgcc tgaacacata aagctgatca agcagatcat aaagaagtat actgccgctt
    25201 gtgaaccgaa caattcgatc cccatcacaa agcaggcaat cggtgtattt gtcgcaccag
    25261 aaaatactcc gataaagccc aatcctgcaa gaaaaggaac agaaacatgg agcaaaggcg
    25321 ctagtgctgc accaagcgtt gcaccaattt caaacaatgg tgtcacttct cccccttgat
    25381 atcccgcgcc taaagacaaa actgtgaaga ataacttccc aataaaatca tagacatgtg
    25441 cctgtccatt aaatgattct tgaagtaacg gaagactcaa tccgagataa cgttgtgttt
    25501 gcaaaatcaa tgctgctgcc acaactacag ctccacctat cgtatttcgc caaacaggat
    25561 gaggaatcca ttgtgcatac cgtgctttta aaaagacgat cgaacggctg aatacccagc
    25621 caatcaaacc aaaagcaatc ccagcaacaa aaactttgaa gaacagcaac atactccatt
    25681 cagggatcac acccatttgg taatgtaggt gagaaacacc atagctttct gtcataaaat
    25741 ttgcaaacag tgcagcaaag aagctaggga agatcgcttc tgttcggact ttcccgattg
    25801 ccaacacttc taatccaaat aatgttccag ctaaaggtgt gccaaataca gaactaaaac
    25861 ccgcactgat tccactgata atcaagattt cccgttctgc tttatccaat cggaacaaac
    25921 gtgctgtatt atctgctaac gctcccccca tttggactgc tgttccttca cggccaacag
    25981 atcccccaaa taaatgggta gttatggttc caaacagtgt aagtggaata agtcgcaacg
    26041 ggattttttc ttctccacca tttccttgat cgatcactaa gttgtttcca cgactagaaa
    26101 gaccgccgtg atacgcatag agataagcaa aaagcgcacc actaactggt agtagataaa
    26161 ggagccaagg atgtgatagt cggatatctg tcacaactgt caggctattt aagaaaaaag
    26221 cagacaagct ccccatgaat ataccgagaa tcgctgtgat caccagccac ttcagcatgt
    26281 acactaaaat gcgtactgta ctcggtaatt gtttttccat aaaaaattcc tcctcaataa
    26341 aaaaacgcct actaaagaaa ggggctgtcc ccagacttta gtaggcgtca ttagctatct
    26401 ttcgttagca tttaaaggcg aacaccatca cctattcact tccattaaaa gatagcatat
    26461 ttttctgtaa aaaacaataa aattatcgtt ctttctttac acgttcagca aattcataga
    26521 aaggagccaa tttcgttaac tgttgattac attggtcact atcatgacaa atataatttc
    26581 cctttttcgt atacgtgcca tcagaacctg ctttcgttgt agaaagaaaa agcgaaacat
    26641 tcgatgtttt ctgacagatt gcacagattc ccttatttat cgttggtgat aaagtaccgc
    26701 taatcccttt taatttttca tcttcgtaat agaccatgaa ttttttctgc gtacctggat
    26761 cattccagcc gatgtaagta tgttcttgca agtcgatttc attccaagct ggttgtttga
    26821 gttttttcac ttttcggaat aatttctcga tttgtggttt gctaggctgc ataaacggct
    26881 gaacgatttg cttcagctcc tccaatgctc tttctgcttg cgcttttgtc agttttacgt
    26941 ctaaaactgt cgttaaaaat gtatcgattt ctggcagttc gtgatctaat atgtctcgaa
    27001 tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg acagatgagt
    27061 acgcatttaa taactgttct acttctcttt ttataaaaaa atattggtat ggttttattg
    27121 tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg tagaaaaaag
    27181 tgatcttctg caaagtatgt gttacctgaa gatcccggac tcttttaaat aagatacgtt
    27241 ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat tcattcgata
    27301 aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa aggactgtga
    27361 caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct tgcatctgcg
    27421 gtaataagcc gaaacttcac aaaaatttga gaagtaattt tcgtaaattc cttcttattt
    27481 cttgaagctg accacttctg tcacaacctc aatatggtct attattggtt ttacctgtta
    27541 aatcgaggat tctttcccaa agtaacgttg ccatctctta cgcatacttg cagacatggg
    27601 ctgagccgct tctttgactg cgcagtattt gtccacgaat gtcacaatat atccttcttt
    27661 atacttaggc ggtgcgatcg ttgcacccca catgtgcttc aaaatgatat ctcgctcaag
    27721 atccgacaaa tcggtgattt tttctgcatt ctttacagca attcttggat ggatatacgc
    27781 atgtgtcccc tcgtcaaatt tcgtcgtacg ccaatcatag tagaacagat catgcaaaag
    27841 tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca gataactata
    27901 gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg tgtaatttgc
    27961 taattttttc actgcttctg tttctaaaag atcttgtaca taagacatat attcttcgtc
    28021 ttcacgccat ttttctgtca taattatcga tctgccacct ttttgaattt cattttcttc
    28081 tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgctct agataaaata
    28141 gctaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacgat ccctgctgcg
    28201 actccgacat tcaatgattc cgcatttcct ttcattggga tatatacgtt ctgatcagtc
    28261 aaagacaaga tttcctgact tactccctga ccttcgtttc ccatgattat agcgaaatcc
    28321 tctgtttttt ctactttgtc taatgcatgt gcttcttcat ttaattctgt accgtacacc
    28381 gtaaaccctt tgcctttcat tacagggatc acttctgcca aatctttttg taacacaggc
    28441 aaatgataat tgcttccctg catgcttcgc agcacctttg tactataaat atctgccgtt
    28501 cctttaccta ataaaacacc agccatacca aacgcatctg ccgtacggat catcgttccc
    28561 acatttccag gatcctgtac attatctagt aaaagccagc ctcctgaaaa atctggcgta
    28621 tctttagatg ttaacgtcat cactgctaag atcccttgcg gtgtaggtag atctgacata
    28681 gcggtcatga cctcatctga aactagaaaa gtagaaatct ctttgtttac agccagccag
    28741 tctccccatt ctttcatacc tctctgtgtc acaaaaagtt cttttatttc tacatcagac
    28801 gacgcagctt cttcgatcag atggaaccct tccaaaagat attctgcctt ttcttctctg
    28861 tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttgc tgattggatc
    28921 tctttcacat tccttcacct cacctgatta tatcatggaa taggtgcttg agcttgtttc
    28981 atcctaaaaa aatcggtatc attgatttat aaaacaggaa agaaaggtga cgaacatgcg
    29041 caaagttaaa atgaatgtcc aaggacgtgt ccagggcgtg ggatttcgct atatgaccaa
    29101 gatggttgct gatcagttag gcgtgaccgg aacggtaaaa aacgaagacg acggctcagt
    29161 cacaatcgaa gcaatcggaa atgacgacat catccaaaaa tttatcgaag aagtcaagaa
    29221 gtctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaggatc cgatgattga
    29281 ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaggtcgctg agttttgagt
    29341 attaaggaaa attttgtgaa aatagctttc cacatttttg cataaatttg acttaatacc
    29401 gagaaactga cttttgaaca ccgtcgatca ggttgcgaaa aagctgatct gcatcaagca
    29461 ttaaggactg gttgtgaaaa agtcgcttag ttttgagtat taagaaaaat tttgtgaaaa
    29521 tggctttcca catttttgca taaatttgac ttaataccaa gaaattgact tttgagcacc
    29581 gtcgatcagg ttgtttttga ggcgctttgc ctcaagtatt aaagataggt agtaaaaaag
    29641 ctgatctata acttgcaata aatttgctta acccatattt tccagacaga acaaaaaagt
    29701 ttttagtttt ttaaataaga aagaaacact tggaatcact cttttctttc caagtatttc
    29761 tttcttgatt ttttgtcttt tttcaaaatt tttcgctacg aaaaagaaag aatatttata
    29821 tgaaatttct atgaagttga ttgaattcca tgcgcttttt tagtatagtt aagtttgtgt
    29881 aaaaattata gaagaggaag tattctatga ataaatggaa aaactggtta ttgggatccg
    29941 gacttgtcag tatcctgctc tttttatctg gctgtgtgcg gatggattca aacggaaacc
    30001 ctgacacttc aggaatcgtt tatcgagtac tcgtccatcc tatgggccaa gcgattacat
    30061 atctagttga gaatttcaat tggtcttacg gctgggcagt tattgcaatg actgtgatcg
    30121 ttcgtatcat catcttgcca ctaggtatca gtcaatcgaa aaaaacaatg atccaatctg
    30181 aaaaaatgca agcgttaaaa ccacaagttg aagcagcaca gcaaaagcta aaagctgcaa
    30241 caacacgtga agaacaaatg gcagcacaag cagaaatgca gcaagtatat cgcgaaaacg
    30301 gattgagtat gaccggcgga atcggctgtc tgcctctatt gatccaaatg ccgatcttct
    30361 ctgccttgta ttttacggct cgctacacag aaggtatccg tgaatcatct ttttacggca
    30421 tcgatctagg ctcaccaagt atggtgcttg tggccatcgc aggggtcgct tatctgcttc
    30481 aaggatatat ttcaacaatc ggtattcctg aagaacagaa aaagacgatg cgtacgatgc
    30541 tgatcgtctc tcctgctatg atcgtcttca tgtcgatcag tgcgccagcc ggtgttacac
    30601 tttactgggt agtcggtggt gtgttcagct gtctgcaaac attcatcaca aacgttatga
    30661 tgaaaccacg tttgaaagca caagtggcag aagagttgaa acaaaacccg ccaaaacaag
    30721 tcgtcactcc aagaaaagac gtgacagaat cagcaaagcc agctgacaac aaacaagcaa
    30781 caaaacaaat caaacaacca aaaaacagta caaataacaa aggccgcaac gcaggaaagc
    30841 agcagaagcg gaagtagata gtaaataaga gtgtaataaa aattcattaa cagttttagt
    30901 aattaaaaat atcgaccaag atttttgaaa aagaaatctg gtcgatattt ttatttaagt
    30961 gaatgtttaa tttgaatcat cgattgatcg ctatctaatt gcagcagcat ctttttatga
    31021 agaaacaact ttttcttatc tatcaacgta gacaggtgtt gctgtttctt ctaatagtgt
    31081 acttaattct ttaatatttt gttttccttc ttctaacatc caactactcg ctttttcttc
    31141 gccttcggta acaaaaggtg ctactccctt cgcccatgtt gctcgtccac ataagacacc
    31201 attgaatgtc gaacctgatt gttttgcaaa acgcaatgta tcttgaaata gtgcagcgct
    31261 gactcccgca ctaaaaaaaa taaacggttg ttcagttact ttcgattgtt catagaaata
    31321 agtggatgcc tctgcttgac tataacaagt ctcttgccca tatcctttca caaatctcat
    31381 attcactggt acttctactt tcaatacatc cacttgataa cgttcttttg aaaattctcg
    31441 catggcttca atcactttat gcggttttac ttttgcatag gcaagagaag acggatcgct
    31501 aatagttgca tcataagaaa ggatctctaa aaagaaaggc ataccttctg cttcgcattc
    31561 tgaatcaatc cgttctataa ctatttgttt ttgcacatta atctctactg attcatcaac
    31621 gtcatagtac aataaaaatt tacaagcatc cgcacccact tcttttaacc ttttcactga
    31681 ccaactattc agtaaatctg gtagtcggcc cggcattttc gcatcatagc cggttttttc
    31741 ataagctagc aacaagccac attctttctc tcgaagcgct gctgcaggca gaccatattc
    31801 tggatcgagt aaaatagcgg aagcatacgg agtcaggtac tgagaaacca tctgtttaaa
    31861 ttggatgata tcttctgatg tagcttcttt atattggttg atcatttttt tcaaagcacc
    31921 acgctggtcg attgctaacg cgctaataat tccagcttca tccgtcaatc gcttcaaaca
    31981 ttcctgtttt tctatactca ttttcttcat atatcttcac ctatttcttt agttaatatt
    32041 gtggctattt ctgtttttga ttttgctgct ctcaactgtt ctgtaacctc ttttcgcatc
    32101 aatgatcgtg caacgagaga caataatttc aaatgagtcg ttcctgcttc tgaatctggg
    32161 ataaataacg caaaaataaa atcaactgac tgtctatcta aactgttcca ctcaatccct
    32221 ttgttcagtg tgacgataat caaactaggt tcgttgattg tttgagactt cgcatgaggg
    32281 atagcaaaac ctgacattat tcctgtagta ccttcttttt ctctttctag aagttttcga
    32341 tatacttctt tttcgtcatg agcgattcct aaacagaatg tttgctttgc taaaaactta
    32401 aatgcttctt cttgactatc taatggctgt tgtaaaaaaa tgttctcttc atgaatgatt
    32461 gtcatgataa gcctcctgct cttccttctt cgataactgt acgatttgct gctttattat
    32521 ccatcttctt gtctttatca gttactcttt tcttttggat caaacctagt atcactccac
    32581 caacgatcga accaataaga atcgctgtaa cccattggat ttttcccgtc actacaggta
    32641 acactaaaaa accgccatgt ggggctggta cttggacacc aaacagatag gtcaagacag
    32701 cagcaattga tgagcctaac atcatcgttg gtaatactcg gatcgggtct ttcgcagcaa
    32761 acggaatagc tccctccgta atatgagttg atcctaaaat aaaattaacc aaacctgcat
    32821 tacgatcatt agtatcaaaa tatttaccaa acaataaagt tgcaaaacca gtaatcaacg
    32881 gtggtgcaat gcaagctact gacacgccag ccataaaagt cgtgtttcct tgtgccaaaa
    32941 gagctgtacc tgttacatat gccgccttat taatcggtcc tcccatatca aacgcacaca
    33001 tgcaaccaac aatcacgcca agaacaagtg gattcgaatg ttcaaatcca gctaaaaagc
    33061 tcatcattcc ttcatttata gccttcattg gacccgccag caaagacatc acgatgccga
    33121 taaacactac accaataact ggatataaga agattgcctt caagccatct agttgtttag
    33181 gtaaaattgt cagtagcttt tgcaaaaata agataaaata acctgctaag aacccagcta
    33241 aaatacctcc taaaaatcca gtaccgccac tcatggccac gattccacca acaaatccaa
    33301 cgactaaccc tggacgtttc gcgatccctt ctgcaatata agctgctaaa actggaacca
    33361 tcatccccat tgctgcatcg ccaaccgttt ttaacatcgc cgctgtagca ttgtattgtg
    33421 catggtctgg atcaaaagaa tagattcccc aaacggcaaa tgaaatagca atcaatactc
    33481 cgccagctac cacgaaaggc aacatatgag agacgccatt cattaaattt ttatagatgc
    33541 tgcgaccaaa attagcacca cttatcgttt ctgattctaa agtatcaaca gcttcattat
    33601 ctgctaagat ggtccctttt cctgccaatg cttcgtcaat cagccgttgt gcatccttga
    33661 ttcctgtact gacagaaaca tcaatgactc gttttcccgc aaatctttct gaatggacat
    33721 ctttgtctgc ggcaataatg acagcatctg cctgtttgat ttcttcagcc gttaattcat
    33781 tctcgatccc aatttgtcca tgggtctcca ctttgatcgt gatgcctttt tttctagctg
    33841 cttgctctaa tccctcttgc gccatgtagg tgtgtgcaat tcctgtcgga caaccggttg
    33901 ctgcaattag ttgatatttt cccatctttc gaacctctct tccttgattt tgatttgttt
    33961 acttaattcc tccacatcca aaaaatcagt cagtccttta cgaaaagcag tagagctgcc
    34021 tgctgctatg cttttacgta acgtttcatc aagcggtcgt cgattcatgt atcctactaa
    34081 aaaagtgcct aacatggcat cacctgcaca tgctgtattg acaacttttc cttttggcga
    34141 gtttcctcga atcaccctgt ctttcgtaaa aagaatcgct cctttctcac caagggataa
    34201 caaaacattt tctgcacctg actctaccag tcgttgtcca taaacaaagt aatcttcatc
    34261 tgtaaccatt tttcgtccga accaagaaga aagttcttcc tcattcggtt tcagcaaaaa
    34321 tggtcgatag ggcaaacaac ccatgacctc ttgatcactg ctatcaataa tcaaatgaat
    34381 ctccttttcc tgacaaatcc gactgatttc cactaaaacc ttcggagaca ttcctcttgg
    34441 taaactccca gaaacacaaa gataatcctc tgcttgtaat gctcttattt gatttaaaaa
    34501 attatggaca gctgcctgcg gtatttcagg tccttgattc actaatttgt attcttctct
    34561 cgtttgattg acttgtgtaa atacattgat acgcgtcata ccaggcactt caataaactc
    34621 tgtttggatt tgtttcttct gtaaatagtc ttctatatat tttcctgtga aaccagcaga
    34681 aaaacctaat gccgtacttg ggaaatctag catctttaat actagtgaca catttacccc
    34741 ttttccgttt gcctgaatgt catcctcctt tgtacggttt accataaaag gatgcatctc
    34801 ctctgtttca ataaatagat caattgccaa attcatcgtg catgtgtaga tagacataac
    34861 caatgacctc cttgaatcta ttatgaaata cgcgaaagag aaaatgtttt cttaatcgtt
    34921 ccaacacaaa gaaaaccttt acatttataa acacatcgtg ctatttttta actaactttt
    34981 agtgaaagaa ggaaagaata tggatcacaa attaagtgaa acagaacagt acctctggaa
    35041 ctttatcgaa caccacatcc tagagatccc taactactct atcgtgaaac taagtgaaca
    35101 agcaaatgtc tcaacagcca ccattgtgcg gacaatgaag aaaaaaggct atgaaggatt
    35161 tacttcgttt aagcaccatc taaaagaagg agaaaacaag aacatcaatt tttctttctt
    35221 agataaagta gacaaaggta tccgtcgagc cattctaaaa aatgaacaag aagtggtacg
    35281 tacgatcaat atgttggaaa tcgggaacat tgaagatgca atacaaagaa tcaaatttac
    35341 tgaccgggtt tttgtctttg cccgaggatt ttcagaaatg attggtcaag agatgctcgt
    35401 aaaacttcaa ttaacaggaa aaaattgtca aatgcatact gatccagaga ttatcaaaag
    35461 catcagtcgg aaactaacta agaaagacag cgttattttt gtttctttga acggcgagac
    35521 aaaagaactc gtcaccgccg caaagaactg ctatgatgca gagattggtt cgattctagt
    35581 tacagccaat cattcctcta cactcaaaga ttattgcgag ataaatctcg taggtttcaa
    35641 atcagaaggt tcttatttcc ctgattatga agtacgttca cgcttgcctc tccagatcat
    35701 cgcgagaatc ctgcttgacg cctatgtatt gcgtatgagg gagaattgag aagcagttta
    35761 ttatggttgg ttacagttta aaacatcaca ttttttattc tttatattgt atggactcac
    35821 gtaaactttt tatgtataaa gttattcac
//
LOCUS       DACDNO010000023        35385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDNO010000023 DACDNO010000000
VERSION     DACDNO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.023"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(98..1117)
                     /locus_tag="IWI22_001210"
     CDS             complement(98..1117)
                     /locus_tag="IWI22_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9168611.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1139..2146)
                     /locus_tag="IWI22_001211"
     CDS             complement(1139..2146)
                     /locus_tag="IWI22_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9168612.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2139..2804)
                     /gene="pgmB"
                     /locus_tag="IWI22_001212"
     CDS             complement(2139..2804)
                     /gene="pgmB"
                     /locus_tag="IWI22_001212"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9168613.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2797..5091)
                     /locus_tag="IWI22_001213"
     CDS             complement(2797..5091)
                     /locus_tag="IWI22_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9168614.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5351..7516
                     /locus_tag="IWI22_001214"
     CDS             5351..7516
                     /locus_tag="IWI22_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9168615.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7820..8653
                     /locus_tag="IWI22_001215"
     CDS             7820..8653
                     /locus_tag="IWI22_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9168616.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8755..10455)
                     /locus_tag="IWI22_001216"
     CDS             complement(8755..10455)
                     /locus_tag="IWI22_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168617.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10962..12122
                     /locus_tag="IWI22_001217"
     CDS             10962..12122
                     /locus_tag="IWI22_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9168618.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12138..12581
                     /locus_tag="IWI22_001218"
     CDS             12138..12581
                     /locus_tag="IWI22_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9168619.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13064..13594)
                     /locus_tag="IWI22_001219"
     CDS             complement(13064..13594)
                     /locus_tag="IWI22_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9168620.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13699..14250
                     /locus_tag="IWI22_001220"
     CDS             13699..14250
                     /locus_tag="IWI22_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9168621.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14403..14876)
                     /locus_tag="IWI22_001221"
     CDS             complement(14403..14876)
                     /locus_tag="IWI22_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9168622.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15014..16486
                     /locus_tag="IWI22_001222"
     CDS             15014..16486
                     /locus_tag="IWI22_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9168623.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16483..17193
                     /locus_tag="IWI22_001223"
     CDS             16483..17193
                     /locus_tag="IWI22_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9168624.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17545..17829
                     /locus_tag="IWI22_001224"
     CDS             17545..17829
                     /locus_tag="IWI22_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9168625.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18110..18529)
                     /locus_tag="IWI22_001225"
     CDS             complement(18110..18529)
                     /locus_tag="IWI22_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9168626.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18531..19115)
                     /locus_tag="IWI22_001226"
     CDS             complement(18531..19115)
                     /locus_tag="IWI22_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9168627.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19162..20637)
                     /locus_tag="IWI22_001227"
     CDS             complement(19162..20637)
                     /locus_tag="IWI22_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9168628.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20818..21129
                     /locus_tag="IWI22_001228"
     CDS             20818..21129
                     /locus_tag="IWI22_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168629.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21244..23580)
                     /locus_tag="IWI22_001229"
     CDS             complement(21244..23580)
                     /locus_tag="IWI22_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9168630.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23722..24390
                     /locus_tag="IWI22_001230"
     CDS             23722..24390
                     /locus_tag="IWI22_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9168631.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24393..25436
                     /locus_tag="IWI22_001231"
     CDS             24393..25436
                     /locus_tag="IWI22_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9168632.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25673..26554)
                     /locus_tag="IWI22_001232"
     CDS             complement(25673..26554)
                     /locus_tag="IWI22_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9168633.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26713..27441
                     /locus_tag="IWI22_001233"
     CDS             26713..27441
                     /locus_tag="IWI22_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9168634.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27597..27914
                     /locus_tag="IWI22_001234"
     CDS             27597..27914
                     /locus_tag="IWI22_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9168635.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27907..28515
                     /locus_tag="IWI22_001235"
     CDS             27907..28515
                     /locus_tag="IWI22_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9168636.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28531..29361
                     /locus_tag="IWI22_001236"
     CDS             28531..29361
                     /locus_tag="IWI22_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9168637.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            29499..30011
                     /locus_tag="IWI22_001237"
     CDS             29499..30011
                     /locus_tag="IWI22_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9168638.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            30037..30909
                     /locus_tag="IWI22_001238"
     CDS             30037..30909
                     /locus_tag="IWI22_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9168639.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            31085..31447
                     /locus_tag="IWI22_001239"
     CDS             31085..31447
                     /locus_tag="IWI22_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9168640.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31456..32208
                     /locus_tag="IWI22_001240"
     CDS             31456..32208
                     /locus_tag="IWI22_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9168641.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32222..34024
                     /locus_tag="IWI22_001241"
     CDS             32222..34024
                     /locus_tag="IWI22_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9168642.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34301..35341
                     /locus_tag="IWI22_001242"
     CDS             34301..35341
                     /locus_tag="IWI22_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9168643.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT        11053 a         6255 c         7288 g        10789 t
ORIGIN      
        1 ttttttacag ataaacaatg tgttagaagt agtatcttct tttacataaa aagctgacca
       61 taggataaat ggtcagcgta tttagtgagt cagctattta gtatgggttt tctaaaggaa
      121 cgactgtctc tcttttgatc aagcgatgag ggatgaccaa acgtacgcca ctgctcaatt
      181 cttcatggat caattcgcgc agcttttgca tagcgacacg tcctaattcg gagatatcga
      241 tatcgatact tgtaaggtaa gggtgtgtta atgttgagaa gatcgagttg ttgaagctaa
      301 tgacggagag tgtctctgga atgacatagc catacatttg cgccaactgc atcgtgcgaa
      361 gggcgaagat atcgtcaatg accaccaatg ctgtggcttc gttttctttc aacgttttct
      421 caaaggacag ataatcctcc ggatgctcta gtactacact ggataacgca ggaagaccag
      481 ccatcatcat tgctttttga taaccgaaat atcgttcgaa atacaaactt tcatgagtcg
      541 tgtttgtcac aaacaggata ttcttatgtc cattctggat caaaaattct gttgcttgtt
      601 tacctagaag ctggttatca ttatctacat aaacgatttc gttctcatga tgataaggct
      661 gtccgatcaa agtgaaaggg atgtggtttt ggtataaata atcaatgaca gtatcttgac
      721 tatctgaata cactagaata aaaccatcta cttgtttttg taaatgcata cgctggacgt
      781 tctctaacag caattcaaaa tttttagcag tagcgattgc tgtagtcata tcataattcc
      841 gcgcttcttc gttgattgct tccatgatct caagataaaa tgggttaccg atccgctctt
      901 ttgaatcaag agggggtaag attacgccga tggcactgga aatgcgtttt cctagattgc
      961 gggcagtcat gttaggaaca taacctagtt catccataat ttttcggact ttcttctttg
     1021 ttgtctcgga aatactagga tgatcgttga tgacacgaga gacggtcgag gtggcaacac
     1081 cggctttttt cgccacatct tttacggtaa tagccatgat aaaaacacct ttctcttgtt
     1141 attgtgcaga gaaccgataa atcgtctgac tgatccgttc ttgaccagga tggaggttga
     1201 tcgatccaaa tttggggaaa tgaacgatat cagggatttc ttgcggttcg attgctaatc
     1261 cgtagtttga atgcatctgt tttccattga cagagaaatc agcttcgaat ccagtcgtag
     1321 aaaataatac catactttta ttagaagttg ctatagtcat ttggcgacca gaatgcttct
     1381 cgaataattg taaggaagtt ttggaaagcc taggatgatg caaaagaaat acatcatcta
     1441 gtccatctgg ataacatgca aggatctctt gcagtgttgg ggattttcta aagtcgattg
     1501 gcagtttttc tttttttgtc caatttccag taggcagttt ttcttgatct aattcaagca
     1561 caccatctaa aaagaccgtt aactgatgtt catagatgtc tcttgttcca tttcctgaaa
     1621 ggttgaagta ggcatggttg gtcggattgc agatggtttc aacaaggcta ctagcagagg
     1681 ttgtcatctc taattcgttt tttgttaaac ggtaagtgat cgtagcagtg attggtcctg
     1741 gataggtaga aaattcatct tttaagtaaa agaccacacc tattgatgat ggagttttga
     1801 aaacttctac ttcccaaaat tggaaagacc agcctttcgt gccaccgtgg atatggtgtg
     1861 atccgcagtt tttctccaat ctatgctcac cccaagaagc atcccggatc cttccggcta
     1921 caggtccgac agttgctcca aaaaagcttt tgtctgttag aacatcttcg aaacgatcgt
     1981 aagacaataa aacattttct gattttcctt ctttgtctgg tgcgaagatt tggtgcaggc
     2041 gggcaccgta attgagaaga acgacttcta agtagtcgtt cttcaatgtg attttcttta
     2101 tttcagtctc tggaactggt acgacggaga tctctggatt atccatggtc atgccacttt
     2161 tctttcaagt aatcgatcgt aagaaaagtc gtatcaggga caagcgggat ctcctctcca
     2221 aggtcttcag cacgtcccac gccaattggt tgagcgccac ttgagaggat ggcttggata
     2281 ccggcttgcg cgtcttcgat cccgatacat tcttctgctt ttagtccgac agcttttgct
     2341 gcaaggagga agatatctgg cgcaggtttt ccgtttgttg cttctgccgg gtcggcaata
     2401 gcatcaaagt aaggtgtaag acccattttt tcaagtagga atggcccgtt tttacttgca
     2461 gaagcaagag caatcttgat tttttcttct tttaaagaat ccagtaaagg cacgaccccg
     2521 gggaagacat cttttggttc gatcgtctgg atcatctcta aataataatc gttttttctt
     2581 ttcgctaatt ctgcaaattc ttcctttgaa aaagagtgtt cttttccgcc ataagctaaa
     2641 agcaaagaga gtgagtcttc tcggctgacg ccttttagct gttcgttgaa tgtgtggtcg
     2701 atcgtaatgc cgatttcctg accaagcttt cgccaagcac gataatggaa ttcagcagta
     2761 tcggtaatga caccgtctaa atcaaacaat acccctttaa acatttgctt cctcctttct
     2821 tagcgttatt tctagacgat ccgctaaaga ataagactgt tcatagacgt aaatttctag
     2881 aggagaacct tctaataact cgaaagtaac ttgttcatct gtgactgaga tgaacaataa
     2941 acgtccgcga taattgatat ggaaagaata agctttccaa gtttttggca gaaatggtgc
     3001 gaatctcaac tgatcatggg cagttttcat ttgagcaaat ccttggacaa tcgacaacca
     3061 gctaccagtc atggaagtga tgtgcaagcc gtcttctgtg tcgttattgt aattatccaa
     3121 atccaagcga gctgttcgct ggtacatttc tacagctttg ctttccattc ctaattctgc
     3181 tgctaaaatc gaatggatac ttggcgaaag ggaagactca tggacagtca ttggttcata
     3241 gaaatcaaag tttttttgct tttcttcttt tgtaaaagta tcattgaaga aataaatccc
     3301 ctgtagtaca tctgcctgct tgatgaagca agatctaagg attttgtccc aagaccaatg
     3361 ctgattcaga ggaagatcgc ttgtgggaat atcggataca ggcatgagat ctttatctaa
     3421 gaatgtgtca tgctggacaa agattcctaa ttcttcgtca actgggaagt acatgttctc
     3481 tgcgatatct ttccatttgg ctagttcttc ttcgctgata gtgatagcag cttctttttg
     3541 ataagttaaa taattttctc gcgtgtaacg caagacccaa gctgcgatcg tatttgtata
     3601 ccaattgtta ttgatattgt tctcatattc gtttgggccg gtcacaccgt ggatcatgta
     3661 tttatttgct cgttttgaaa aatggacacg gtctgcccag aagcgtgcga tttctgctaa
     3721 aacttccagt ccttcatttt tcaaatattc cgtatcgccg gtgtaattcg tataattgta
     3781 gattgcatag gcaatcgcgc catttcggtg aatctcctcg aatgtgattt cccactcgtt
     3841 atggcattcg acaccagtga aggtgaccat aggataaagc gcgcctttta atccttgttg
     3901 ctgagcgttg tggattgctt gcggcagttg attatgccga tatttcagca gattcttagt
     3961 tacttcaggt ttggctaaag caagataaag agggacagca tacgcttctg tatcccagta
     4021 agtggctccg ccgtattttt ctcctgtgaa tcctttgggg ccgatattca atcgttcatc
     4081 ttcgccgtag taagtagaga atagttggaa caaattgaat cgaatacctt gttgtgcttc
     4141 ttcgtctcct tcgatgacca catcggcaag cttccatcgt tctgcccaag ccgctgtttg
     4201 ctcttctttt gcttggatat aattgtttgt ctggttcgtt aattctgaga cagctttttc
     4261 tgcttgctgc gcttctggaa tatctctact agtaagaacg ataatttctt tttccactga
     4321 ccatgtctca ccttgagata attctgcaga caagcaacca tgtaatgcta aagtatcttc
     4381 gtgatagtca gggatgactg attttccatt aacgaaatgg cgcattgctg cagtaacagt
     4441 aaattgttca atcccaaagt tgtttggaat agttttagtc gtcaaatagc cgatttcttc
     4501 tatagttcca cgatcaatct cattccaaaa atgttcatca tagttgctgt cttcgttttg
     4561 aacatttcca tccaattttg atacgagttt gactgttccg ctaccttcaa gcatttctat
     4621 agttaaatag atataagctg attctttttt gacaatgctc agaaaacgtt cgaaggaaat
     4681 gcgtacttta ttagtagaag tagtgattgt gaatgtacga gataatacac catttttcat
     4741 atctaattcc cagtaaaagt cttctggttc caaagaagcc aagtcgatca gctgatcgtt
     4801 gatcgaaata tctatcgcga taaagttcaa ggcgttgatc acttttccaa agtattctgg
     4861 ataaccattt ttccaccagc cgacacgtgt cttatcggga taccagacac ccgccaaata
     4921 agtaccttga tggtggtccc ctgagtaagt ttcttcaaag ttcccgcgca tccccatata
     4981 accgtttcca atacttgtta aagattcttg cagtcgaaga ttttttttat ctaattgtgt
     5041 cgtgctgatt ttccatggat caatagcaaa tagtcgtttg atctgtttca tttttctcct
     5101 cctaatgagc tgttttatac gttcataata aacgcaaccg attgcacaag tcaatagaca
     5161 aaagaaaata tttcatagaa aaaagcttaa aaaaataaat ttttaggaga aaattataga
     5221 agggaacaca cgtattatag gttttataga atggtataga cagaaaaata aaaaataaag
     5281 catttacaaa aaagaaaacg cacgctataa tacgtttaac gaaaccgatt gcgtaaagga
     5341 gctaaacgga atgaaaaaga tatttagttt tgaattttgg caaaaatttg gaaaggcttt
     5401 aatggttgtg gtagcagtta tgccggcagc tgggttgatg atcagtatag ggaaatcaat
     5461 tcccttgatc aatcctgatt ggacgccgct tgtcacaacg ggcggagtaa ttgaaaatat
     5521 tggttgggca attattggta atcttcatat tttatttgct ttagcaatag gaggaagttg
     5581 ggcaaaagaa agagcaggcg gagcatttgc agctgggatc tcatttattt taatcaatcg
     5641 gatcactggg gctatctttg gtgtcacaag tgacatgctt gcgaatgaag atgcatttac
     5701 ccacactctg ttcggtacga agatcatggt taaaggattt tttacaagtg tattggaagc
     5761 accagctttg aatatgggag tattcgtagg gatcatcgct ggatttgtcg gggcaatggc
     5821 ttataataaa tactataatt atagaaaatt gccggatgct ttgtcatttt tcaatggaaa
     5881 gcgctttgtt ccgtttgttg ttatcttatg gtcaacaatc gtggcattga ttcttgctgt
     5941 ggtgtggcca aacgtacagg ctggaatcaa taattttggt ttatggattg cagaatcaca
     6001 agaaagcgca ccgattctag caccgttttt atttggaaca ttagaacgtt tactattgcc
     6061 atttggattg catcatatgc tgacgattcc tatcaactat acacagcttg gcggatcgta
     6121 tgagatttta tcaggcgcac aggcggggac acaagtattt gggcaagatc cgttatggtt
     6181 ggcttgggcc actgatttag tgaatcttaa aggtgcaggg gatatgtcgc aatatgagtt
     6241 tgtgttgcat aactggacgc cggctcgttt caaagtagga caaatgattg gttcttcagg
     6301 tatcttgatg ggaatggctt ttgcgatgta tcgtaatgtc gatcctgata agaaagcaag
     6361 atataaatca atgtattttt cagcagcgtt ggctgtcttt ctaacaggtg taacagaacc
     6421 attggagttc atgtttatgt ttgctgctgt gccattatat gtcatttatt cagtgatcca
     6481 aggggctgca tttgcgatgg cagatatctt gccattgcgg gtccattcat ttgggaatat
     6541 cgaattgctg actcggacac cgttagctat caaagcgggt ctaggtggag atcttatcaa
     6601 cttcgtgttg tgtgtgatcg cttttggtgt tgttacgtat ttcttagcaa atttcttgat
     6661 caaaaaattc aactttgcta caccgggacg taacggaaat tacgacaatg acagtgaaga
     6721 aaccgttgta tctaacagcg gaacaggaac agcagatcag caagtggtcc agatcattca
     6781 tttgctggga ggaaaagaaa atatcaaaga tgtcgatgct tgtatgactc ggttacgcgt
     6841 aagtgtaaat gatcgagaaa aagtagggac tgaagaagcc tggaaacgag caggggcgat
     6901 gggattgatc gtcaaagaca atggagtcca agcagtttat ggaccaaaag cagatgtctt
     6961 gaaatcagat atcgaagatc tgctgcaatc aggagcagag attccagagc cagaaatggt
     7021 ttctacagga aatggacaag caggtagtaa gcaatactta ggtatagaac aagaactagt
     7081 ttccgcagct tctggagaag tgattccttt atctgaagta aaagatccgg tattctcaca
     7141 aaaaatgatg ggtgacggct ttgctgttat tccaacatca agagaagttg ttgcgccgat
     7201 cgctggaaaa gtgacaagta ttttccctag caaacatgca attgggctgg aaacgaaaga
     7261 tggtatcgaa gttttgatcc atatgggaat cgatacagtc caaatgaatc agccagcttt
     7321 tgagattgct gtcaaagaag gccaagaagt aggaacagga acgaaattag ctgagatgga
     7381 tcttgatgtg atccaaaacg aagaaaaaga tccaacgatc atgatcgtat ttacaaacga
     7441 taaagtagaa gaagtagtga tcaagcaatt aggtacagtg acagctggta aagtgatcgg
     7501 ctccattaaa atgtaaggag aggttgtgaa aaagctgtcc tgcatcaagt cataagaaca
     7561 gccaaacaaa aatagctcct cctatttttc gtttggttgg gcttattacc gagaagcagc
     7621 agtttgaata ctgtttagtg gagaggtcgt gaaaaagctg ccctgcacca agtgataaaa
     7681 acagccaaac aaaaaatagc tcctggtcca ttgagcttat tactgaggag ttagcaggag
     7741 aacactattt gctggagagg ttgtgacaaa aattttgtca caaccctttt gtttcgaaat
     7801 gaagacaagg agaaaaccaa tgaaattcat gacactcaac acgcatagct ggcttgagcc
     7861 agaaccagag aaaaaattgc aagaattagc agataagatc ctgttagaag attatgaagt
     7921 gattgcttta caagaaatca accaattact ggaatctgaa gaagtagaac ctgcgaagct
     7981 aatgaagttc tgtccggtaa agaatcaagt acctatccgt aaagataatt ttgcctaccg
     8041 tttagttcag cttttaaagg aacacggaaa agagtatttt tggagttggg aaatgagcca
     8101 catcggttat gataaatatg aagaaggaaa tgcactgctt acgaaaaaaa cattggaaag
     8161 tcaggtcctt accgtctccc aatttcaaaa gaaagaagat tatcagacac gcaagatctt
     8221 gattgggaaa acaaagatag atgatcagga catttttgtc tcgagctgcc acttttcttg
     8281 gtggacagat aaaaaaagtg gcttttattt tgagtggaaa aacttggaaa actacttttt
     8341 ggaaactcgc gtaccacttt ttttcctagg tgatttcaat aatccagtag actctcaagg
     8401 ttattatact gtaagagaaa gttgccttct tcttcaagat tcgtatgttg tcgcgaatga
     8461 aaaaggaaaa gcagctactg tggaaaagaa aatagatggt tgggaacaaa atacagaaaa
     8521 attgcgtata gattttattt tcgtccctga aggaatgcag gttaaaaaat atcaacggat
     8581 ttttgatggg atcgatagtc caatcatcag tgatcactac ggggtggaaa tcgaattaga
     8641 tgtgaacgaa taaaaaatat aacatataac ataaaaaaac tgattaaaat aaaactctcc
     8701 aaacgattat accgtttgga gagttttttg atcggatgta tttatccctt ttgcctaagc
     8761 acgtaaaagc ttgtatctat gataaataaa taaacatagc aaggtaaacc agccaatcaa
     8821 agtaagagaa agaatgaaat agaaatagat tggcgtttga taatgaagcg tgagctcgtt
     8881 gattcctttt tgctgcttca cgattggggt cccgatatct gttacttcat aatcagtcaa
     8941 tgtttttcca ttttgctgta gcacagtccg atcgtagacg ataacaggga tatgaacagt
     9001 tttgtcccca gtgtttttcc acttgattgt caaaagaccg cctggatgtg tttttataaa
     9061 agttgtttcg ttttctagta tttcttcgtt ataccggtcg tatttattgt cttttgtctc
     9121 cttatagatt ggcagatagt caggtgtggt tttttggaaa acgaacaagc tggtagacat
     9181 atctttgctg aagaatgttt tccttgcttc ttccggcgta tcgaatagat aagtatgtcg
     9241 actgacaaat gtttcatttt cccacttttc cagatgctgg tataagttct ggatcgtctg
     9301 tgctgaaaaa ataagaatgc caaatatact taaaagacgg aaccactttt gttcagacca
     9361 ctggttcagt gaaagggcaa ggtaaagtag aagcaatagc gtaaatggca cgaaaaatcg
     9421 aaatggaaac tggatcaaac taataaaagg cacatttttt tcgatcaatg tcgtccatgg
     9481 aatcaagcta gtagaaagaa aaaagaaaac aagacttgtg tacaacacga ttttttggaa
     9541 taaggtgttc ttttgttgaa agaaaataaa aagcaaaaaa cctgataata tcaatatagg
     9601 aaaggcctta gggtaatgga gccaataact gccatccaga tctatcgcct tttgactcat
     9661 ttctctgtta acaaagggag gaagaatctt attcccactg taaacaatag ctaatcctgc
     9721 ccaaatattt gctgtcagaa caaaataaag caagaccgac ttaatcagtt gaagcagtgc
     9781 ttttcctttt tcagtctggt taaaaaagta ataaagataa aaagggatat acatcaaagc
     9841 taacatgatc gaagacagta aatggatctg tgtcataagt cccactgaga cagcgacttt
     9901 gataacaggg aactttttat gaaggacgaa atcagcaata gggatcatac ataaaggata
     9961 aaaacaagtc ccccagctag tgaatccttg gttgatagac cagtattgaa tagaatacgt
    10021 actcatgaat aaaacggcta ctggtacact tacccaattt cgaacattga catatctcag
    10081 taaaatcagc atagaaaatc ctgaaataag ataaagaatg aaatttgaaa gaatctgata
    10141 atgaaaccag ctacctgcaa gcaacacgat caatccttgt aaataagcga aaaatgggcc
    10201 gtaaacggca ttcacgatcc tgccactttg ctggaatcca taaagggaag gaaaataatg
    10261 gaaatcccca tatttaattt gttgcgcggc atcatagaac cgattgtaat ggaatggggc
    10321 atcagcaccc aaaattagtt tatgtgtgaa taaatgaggc atgagaaatg ccaaagcaat
    10381 gaaaaaaatt gctaaaaatg ggccatattt tttcaattgt cgaactcctt tgcaaacttt
    10441 agataaattc atcatatatc ctatgcgaga ggtaggcaag aaattagaaa actcatgaaa
    10501 atatttatga attttacaag aaaaacataa gtgaaagttc taaggagaga ggatgcgaca
    10561 taagttttgt tgcatctcct tgttttgaaa cagaattttt cttaatactc gaaacaaagt
    10621 agctttttca tgacctctcg gtaaacggtg ttcaatcagc tgcttctcgg taataagctc
    10681 actagcctag aaaatatgag aagctatttt ctgactagcg ctcttattac tcaaagcata
    10741 acgcagtttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    10801 gcccaaccca acaaaaaata tgagtagcta tttttttggg ctgtacttat tacttgatgc
    10861 aggacagctt tttcacaacc tcatcggctt tcattttcta tttttttcct gaacccatat
    10921 aataaaagaa agaacaaaaa aacttgcaag ggaggacaca tgtgaagcag agtaagcaaa
    10981 aacagtctcg tcgacttttg tttcgtttgt ttttattaat atgtatcgtc gtagggttac
    11041 tgatcgcttt atatccattt tatgtggatt ctttaaatag ttttattgat caaaaacgta
    11101 tcgaacaagt acaaaaacgg accgcagctg aaattgaagt gcagcgaaag aaaatggagg
    11161 agaaaaacca acgattaact gaccaaggat tcaatcctgg tactgatcct ttcaatgaaa
    11221 agaacagaaa tgaaagtaca acatccagtc aattagagga atggctgatc ggaagtatca
    11281 atatacctaa gatccaatta aatatttcac tttatgatcg gctcaatggt aggatccttg
    11341 aaaatggagc aggcgtcttg caaggaacct ctttcccgct tggtggaaat tcgacgcatt
    11401 cagtgatctc tgctcacagc ggtttaccta atcgacggtt atttaccgaa ttagatcgtt
    11461 tagaaaacgg agacacgttt attttgactg ttttggggga gaaattggct tatcaagtta
    11521 aaaatataca agtggtactg ccagatgaca cgtctgttct aacgatttca gaaggagaag
    11581 acttagttac cttgcttaca tgtacgccgt atatgatcaa cacgcatcgt ttgctagtga
    11641 caggacatcg gattccttat aacgaatcgg taaaaaaaga agaaggaaaa ggaaatcagg
    11701 aaagaaccat gcggcaatgg tttattcttg caggaacgat aatagctgta gtcatactgt
    11761 tagtgtttat cggacgacta atctatcagt atagattaag taaaaaagtg cttgattttt
    11821 catttattat tagtgattcg tcagggaaac cggtaaatgg tagttcattc attttgaaac
    11881 ataagaaaaa aacacttact agaaatggtg tgccgttttc agtacaaagc gaccatcatg
    11941 ggaaagtcaa gctggatcaa ttgcctggtg gaacttatcg aatcgtttca gctgatgatc
    12001 ctaaagttgc tgtttctttt ggtattagaa aagtaaaaca agagaagata tacttttttg
    12061 aggggcgaaa acttgtaaaa gagcttcaaa aaaatggttt ttattttaaa atcaatgaat
    12121 aagtaaggag agaaaaaatg atccgcacaa caacaaaatt gtctgccaaa gaactggaat
    12181 ctgttctatc gatttggctg catgctaact tggattctca tgaattcatt ccatcaactt
    12241 attgggagaa tcaagtagaa tctgttagga aagctttacc agatgcagtg atttacttag
    12301 cagaaataga tcaagaaatt attggttttg ctggggtgat tgatcagttt atcgcaggaa
    12361 tctttgtgaa aagggaatac cggcaacaaa gaatcggtac gcaactaatg aaaaaaataa
    12421 aagaaaccaa taaaacactg actttgacag tctatcaaaa aaatcaatca gctattcatt
    12481 tctatctttc acaaggtttt acaattcaaa cacagcaaaa agatgaagca gtaaatgagg
    12541 aggaacttgt gatgaaatgg aatcaaaacg ctgatttttg atgaacagaa taacgaaata
    12601 atgctcccag cctaattttt tgctgggagc attatttcat tttgaagtca ctaatttaac
    12661 tgtgtgaata tgcagttgtt acaatctttg ataaacggtg ttcaatcagc tgaccctcgg
    12721 tattagctca aaaaacggca aaatatgagg aactattttt ccatttgttt ctctaatact
    12781 caggtcaaaa cgctgatttc acaacctctt gataaacggt gttcaaacgg ctgcttctcg
    12841 gtaataagcc cactagccta gaaaatatga aaagctattt tctaactagc gttcttatta
    12901 ctcaaagcat aacgctgatt tcacaacctc ttgataaacg gtgttcaatc agctgaccct
    12961 cggtattagc tcaaaaaacg gcaaaacatg aggaactatt tttccgtttc tttctctaat
    13021 actcaggtca gaacgctgat ttcacaacct ctctttcaca accttatttt actcgtttat
    13081 aattttcatc gaagtatttt ccgctacgga tatcatctgg cattccatcg tatggttctt
    13141 cgctaataac atcttcattg tctatacctg catcacccat ataagtgatc gtagcaaatt
    13201 caggaacaag taattttgga taagtttcat atttttcacg agctgcttcc atttctgcga
    13261 tatgttcgtt ttggaaagta acagtgattt ctggatgttc ttcaacccat ttagggaaaa
    13321 agatcattcc gtgaagtttc ttttcatttg gcataaagtc caatgccacg tctgtccctg
    13381 ttggctctgt ccaagtgatt ttgaatacgc cgtccgtcaa tttaacgata tcagcttctt
    13441 ggtcttttac ccaacggcct ttgaccattc cgccatggat acggtaatca acagtatggt
    13501 catttttggc ataccattca tattcccaac cattgtcata cgtatagata aagtgagttc
    13561 caataaaatc ttccaattct ttaaattctt tcatgtttaa gcatctcctt ataaattaaa
    13621 acatgtaata aactacatgt tttcgattac tcatttatga tagaatggat ttaaagattt
    13681 gtcaaggagg gaaaactgat ggcaagaaaa aatacacttc gatatatttt gttagggtta
    13741 ttaagtaaga agaagatgac aggatatgaa ttgaatcaat cgttcaaaaa tgaaatagga
    13801 gaattttggc aagcaaaaca tagtcagatt tatcctgaac ttgcaaaaat ggaagagcaa
    13861 ggaatcattc agcatcaagt agagatcaca ggtgaaaaat tggaaaagaa agtttatgaa
    13921 ttaacagaag aagggaagga acaattggca gagtggattc atacacccac caatgaacta
    13981 ccggttaatc gagacgaatt tgttctgaaa ttgtatttcg tcaaagacgt gaatgatccg
    14041 gcattagtag aaattattca gcagcagcgt gaccttcatg aagaaaaacg acttcatcta
    14101 ttagagcgaa aaaaaacgat ctttcctact gaaaaagaaa aacatgaaaa ttacggacac
    14161 tacctgattt tagcgcatgc gatcaatcgg gaaacagaat atgcaaattg gctaacagat
    14221 gtattagaag aaagtaaccg aaaaaaataa aaaaatatcc gaacagtgat ggagaattcc
    14281 tttctatatg aaagaaacgc agatcaccgt tcggatattc ttatgttcaa acaggccttt
    14341 tttcaagtat ttatgtagca tctacttatt aaaaagcttg gtacttagga gattagtggc
    14401 gtttagagtt cttttaacaa ctgttcgtag tcagcttttt catcgacagg aaggagcttg
    14461 taagttaatt cgtctgtctc gttgtcatga atgatgtaca tcttagcatt ctcttctttg
    14521 aagttaccga tttgtaaaat cgtatagcgt tcgtcgatca attcgatatc tttgattgct
    14581 tttgaaaaaa ctttacggac atggtgcatt ttcttttggc ggatttctgc cttttctgca
    14641 gcttttgaca aagtaaatcc gtcatctaaa tattttttga tcagttctac ttttatgact
    14701 gtaggtaaac gatattttct aggacaattc gcatcagctt cgatcgaacg aatgaatccc
    14761 ttttgttccc aataccttaa ttgacgtgga gacacaccta cgatatcact taattcactg
    14821 atgccaacaa ccaattgatc attttccaac aaacgtttaa gggaagcccc aaccattttt
    14881 gtcactcctt ttgtcctctt gcttccctta attatatccg tattaactta tgtgtcaagt
    14941 aggttgacaa atagcgaatt taggactaaa gtattctagt agtttcagaa aaaattcaga
    15001 aagaaggaga aatatgagta acaaccaacc cgtagatatt tacggaaaac catataatcg
    15061 gacactttta gttgttgtct tattaattgg tacattttgt accgttttga atcagacctt
    15121 attgacgaca gcttttccta cattgatgaa agctttcgat atttcagcgt ctgatgtgca
    15181 atggttaacg actggtttct tgctagtcaa cgggatcatg atcccaataa cagcatggct
    15241 gatcaataaa ttcagttcaa gaaatttata tctttctgcc atgacgatct ttttgatcgg
    15301 aacgattact tgctttacag cacctaattt tagtacatta ctaatcggtc gtttgatcca
    15361 agcttgcggt gtgggagtat cgatgccatt attgcaaaat atcatgctat ctattttccc
    15421 gccagaaaaa agaggatcag caatggggat gtcagggatc gttattggac ttgctcctgc
    15481 tttaggccct acattatctg gttatatcat tgataactac cattggcgtg acttgtttgg
    15541 tatggtactg ccaatcgttg ttcttgtctt ggttttagca ttcttcctaa tgaaaagtgt
    15601 tattccattg tcaaatccta gcattgatat tttatccgct attttatcaa cgattggatt
    15661 cggtagttta ctttacggat tttctagtgt aggagatgac ggctggggaa gcttgaaagt
    15721 aattggtttc ttagttgttg gtgtgatttt catcgccttg ttctcatggc gtcaattaca
    15781 tttggaacat ccattcttgg aacttcgtgt attcaaatca tctacattca cgattgcagc
    15841 tgttttagct ggtgtaacaa atatggcgat ggtaggggca gaaatggtcg taccattgta
    15901 tattcaaaat attcgtggag aatcagcatt ccattctggc ttgatgttgt tgccgggtgc
    15961 attgatcatg ggggctatga tgcctgtgac gggtgcgatt ttcgataaat acggcgcgaa
    16021 acgtttagcc atcacaggga tgttcatttt gacagcggca actgcaccat ttgctttctt
    16081 gacaaaaacg actccagttt tatatatcgt gatcctttat gcgatccgta tgtttgggat
    16141 ctccatggtc atgatgccgg taacgacttc tggtatgaat gcattgccaa ataacttgat
    16201 cagtcatggg acagcggtaa ataatacatt ccgtcaaatc gcaagctcga ttggtacagc
    16261 tattttgatc agtgtgttga cgaatgtgac aaaagcaaat atcccaagca aatctgtatt
    16321 ggataatacg ccattagctt ataaagatca ggcaatcaat gccacactgt caggttacca
    16381 tgccgcgttt atcgtagcga tcatctttgg gattcttggc tttattattg cctttttatt
    16441 gaagaaaaaa gaacctgttc taatgaaagc gggggataat gcatgattat tggagttttg
    16501 attgtcagct tgcttgcttt tgctttaacg aacgtgttcg ctaaaaaaac atggcaaacc
    16561 tttttatctt tgattttcgg actaatcttc gtcgcttcac taagtttgat cgtcgctaat
    16621 ttgtccaatc actttggaat ggaaaaagta acggaaacaa aaacagaaaa aattgtttcc
    16681 agtgctgatt cccaaggagc agatatgctt ttgtataaag ctttagggaa tggaaaagaa
    16741 aaagtgtatc tttaccgaac aaatgaaaaa caagaaaaac caaaagcaac tggtacggat
    16801 aacgaaacaa ataaagtaga aaagacagac ggcgatgctg aaaaagtgac gaaaactact
    16861 tattgggaat acaaaaacga catgtataaa ttctggttca atattgctga taacaatcac
    16921 gaatatgaca gccgagtgaa tacattcaaa atcccagaga catgggtaga attaagtacg
    16981 gaccaagcag ccaagttagc tgaattagtg aagaaacagc aaagtacgat ggaaagtgaa
    17041 gcaaaagctt atgttcaaga cggtatggta aaagcaatga ctgaaaatcc aaaaatgagc
    17101 aaagcagagc aagaacaacg tacgaaagcc ttagctgctg aattccaaca acaagcattc
    17161 gcaaaattag tgaaagaagc aaaaggcgaa taatattttg aacaccatgt aggaggttgt
    17221 gaaaaagctg ctctgcatca agcagtaaga acaatcagat aaaaatagca ctccatattt
    17281 ttcatctgat tgggcttagt gccgtagatg caagcttttg aacaccgttt aaggaagagg
    17341 ttgtgagcct gccgtttaac tctgagtcac aaggagcaat ttcaaaaaac agcttctcat
    17401 gtttgttgac tattgtgact tgtgaccgag gagttggcag gagaacaccg tttattcgaa
    17461 aaaaggaact gcgacaagac ttttgtcaca gttccttctt ttttttgcta tgctatacta
    17521 gatgtaaaaa ataggagaaa taaaatgagg gaaatttatt cttttgaatc aaaaatctat
    17581 gcttccgaag tcggcaaggg cggggcatat attatttttc cgtatgatat aagaaaagaa
    17641 ttcggtgctg gaagagttaa agtccaagca acttttgaca atgtgccgta tgaaggcagt
    17701 atcgtgaata tgggcgtgaa aaatccagat ggttcagtct gctacatcct tggactacga
    17761 aaagatattc gtaaaaacat cggaaaagaa atcggtgata ttgtagcagt tacagtgaag
    17821 caaaaataaa agaggttgtg acaaaagacg tgtcacaacc tcttgataaa cggtgttcaa
    17881 aagccagttt ctcggtagta agtcaaattt atgcaaaaat gtggaaaccc atgtttacaa
    17941 aatttttctt actactcgaa acgaagcggc tttatcacaa cctcttgata aacggcgttc
    18001 aatcagctgt ccctcggtat tagctcaaaa acggcaaaat atgagaagct atttttccat
    18061 ttttttctct catactcagg tcataacgct gatttcacaa cctcttcttt cataatggtt
    18121 tttcaccatc tttataataa actggttctt tgatatggaa atctattttt tccaaccaat
    18181 ctaaaatctt gtaacctaac tcttctgaca tcggaaaaga ggcgttgatt tgtgcattgg
    18241 agatatggtc aacatggata ccggaagtga tcgtacaact tcccggaacg gcagttttga
    18301 tgatcacagc tattttttcg gctaagacat cgtctttatg gaaccgacca tcgtggctag
    18361 ggaaacgtac agtttgcatc tctgtatcac gagaaaatgt agtgactgtt ccgatatgtg
    18421 gtgtatctcc gcctgttaaa gtgatcaata agtctttacc gattacttta acagcagctt
    18481 ggatcgtgaa acctagtttg gttacttcaa ctatcgtttc tttttccata ttaatcaccc
    18541 tgccaacgtt ttgaatgctc atttacaatt tcaaattgat ccaaaagttt cattgtatgg
    18601 tgattaacaa gatcttggat cgtttgaggc tgattataaa aagcaggaat tggtggtatg
    18661 atttgcacac ctagtcgtgc taatttagtc aagttttcca agtgaatggc atttaatggc
    18721 gtttcacgag gaacaatgac taactttcgt tgctccttca ttgttacatc agcagcacgt
    18781 gcaatcaggt tgtcgcctaa accaacagag atacctgcga ctgttttcat acttgccggt
    18841 acgatgatca ttccatcggt caaaaatgat ccgctggcga cagcagcacc taaatctttg
    18901 tttgaataat ggtaatcaaa caggctttcg aattcagtta gtgataagtc ggattccagc
    18961 tttaagttct gttttgccca tggactgatg attccgtgtg tttctatcat cggatattct
    19021 tttagtttct tcactaggtt gatggcataa atggtgcctg aagcgccact tacaccaatc
    19081 acgatttttt tcttccgttg tgccaattgt tccattttcc gcactccttt ttatgatgtc
    19141 aaaacagttg cagatcattc attatttcaa atatttttcc caatcttcta cttcttggaa
    19201 ttttgcacgt acaaaatgtt ctttcatatc gaaaggaact gtaccgtcaa agatagtttt
    19261 tgcacttatc ccacggaaac gaatagagcc aggattatat tctggtaatt ctgatgggtc
    19321 caatggatgg ttcctcattc ctgggatcac gatgatatct ttatctcctt ggaaacgagt
    19381 gttgaaagtc caaactacat cgttcatgtc gaaaatatca acatcttcat cgactaaaat
    19441 gactgtcttc aattctttga aggctgataa agcaagaata gctgcttgac gctgaatacc
    19501 ttcatccgct tcgttttctt tacggatttg catgatggtc atcaatttac caccacctgc
    19561 aggaggattg tagacattta ctacttttcc tggaatagct ttatcgacca aggcaagaat
    19621 acttgcttct gtaggaatac cggccataga tacgtgttct tcactgggac caatcgttgt
    19681 ttgcatgatt ggattgtctt ttcggtgagt aactgctttg actttcacta cttgtacagc
    19741 tggattagca tccccagtat agccaggaaa ttctggcatt gcttttcctg agttcgtgtt
    19801 gatatcttct tgcattgttt catttggcat gatttctgct tcaatgatga attcagcacg
    19861 agcaataccc acttcatcga ctgctatacc atcaacgatt tgaataggtt cgttacgcaa
    19921 agctcctgca caccatagtt cattgtagcc aagtggagtg gtaggtggtt cgaaagtcgt
    19981 gccaatggca attgctggat ctaaaccaat attgatagtg ataggcattg gtttattcaa
    20041 ggcttcgaat tgtttttgga agtgaccaat gtggcgtccg ccaggcatga tgtacatacc
    20101 gatcgtatct ttatcttcta gaaccatacg atggatcgtt acatcgctca ttgttttttc
    20161 tggatctgat cctaagacta cacccattgt gatgtatgga ccagcatctt caggagtatt
    20221 tgtaggtgca ggcaaaatgt tgcgaatatc gaagtctgca tcggtagcta gatgaacgac
    20281 ttcttgtgct ggagcgtctg ctttgtctac tttcacaggt ttgattggat tttctacagc
    20341 gtctttcaag aaacgaccaa gtgttcgata atcatggtgc aaaatttcac ctacacgttt
    20401 acgactagcc attgttccga tgttcacacg tgtgccgggg aaacctttga tattgttgaa
    20461 agtcatagct ggtccttctt gagtcggacg ttttactgta ccgccagcac cgatataacg
    20521 gtaaacacca gataattctg cttcaggatc gatttcaata tctgtttcgt gatattgacc
    20581 tggaagtgtt tttagttctt cgatgacttt tcttaaatca tacggttgat ctgtcatgag
    20641 agattcctcc ttagttgtct ttacctatct atagtaaaag gtgaaaaaga ggaaaacaat
    20701 tcaacttgtt cggtaaagta ttctacaatg gaatggatgc gtttttttga taatagaata
    20761 aactatgata tacttatcac taaaagaaag cgcatactta gtgttggagg aaggaaagtg
    20821 aaaaaaatgg aggagtcatt tcctaaagca gtaaaagtgg aaaatatagc gaatattttg
    20881 aaagttacat ttgaaaacgg agaagtaaaa tacgtgaaat ctcattggac agaagagata
    20941 accgatgcct tgcaatttgg aaaaaaagga agaggaaaaa gaaaaaatct tctcgcactt
    21001 tctcgtaata tgtggattgg gactgaagta acgattgaag cagatggaac agtattcata
    21061 aatggaaaag acaggtatac gccagaagag ttgtggtaca aaggaaagaa aagtatacca
    21121 gaattataaa aaataattaa aaaaaacagc gcgaaacaag gaacatatta cttcctcgtt
    21181 tcttgctgtt ttctactatt tcagcgctct tatgactcaa atctaaatgt ggttgtcaca
    21241 accttattct gcacgcaaag cgatagcggc gtcttttttc gctgccattc gtgcaggaag
    21301 atgaccgcca agcatcgtca atatagtaga gacgatcact agaataatcg catggacagg
    21361 atttagttga gcaacatttt ttagatccgt catgttgtat aagatgctat tgatagggaa
    21421 cgtagctagc catgcaataa acacacctaa tatcccagaa gaaataccta aaatacacgt
    21481 ttctgcgtca aatacgcgag tgatgtcttt tttacgtgca cctaaagctt ttagtacacc
    21541 gatttctttt gtccgttcaa tcacagatgt ataagtaatg atactgatca tgatcatact
    21601 tgttactaaa gagatccctg caaaagcaat aaggacataa gtgatagcat ccatcaatcc
    21661 gccagtcagt tcagtcatcg ttccagctaa atcagtataa atgattttat cttcatctga
    21721 tttgccttta ttgtaatcat ctagataatc caagatcttt tctttgtctt caaaattgtt
    21781 cggataaatc atgatacttg aaggcaagct gtctccacct aaataactaa gcaatgtttg
    21841 tttggcattt tcgtcaactt tttcagtagt taagacattt acaccactgt ctctttgagc
    21901 tttgacgatt tctgattctt tgttttcgtt gacgatttga gtcgttaatt gatcgctata
    21961 agcgattcct ggagataaga gattcattgt agaagaagat ttcacgcgta ggatccccga
    22021 gatcttcaat tcatcagaag cattctgata catcgcatca taatctgtat tagggataaa
    22081 attcccagtt ggaagttttg tatagtaagt attgttgttt actaatttga atgttgtacc
    22141 aacgatatca tcaaaatcaa gtttttcgtc ttctttcaca tcaaatccaa gattcttcaa
    22201 tgcgttgata ttagtattat tattgccgtc tacgatcagc acgacatctg ttgctgaggc
    22261 tggataactt ccggcaagta aagaataatt gtcttttaaa aagttttctt tgctagtgtc
    22321 aagctgtgta gggaaacttg acactcccac acctgtcatc gctgacatcg tactagataa
    22381 agaaagagat tccgtatcag gattttgatt cgagaagctg acaggctgta ctttgccatt
    22441 tacatcacgt aaaagattga ttcctgtagt acgggtaaat ccgatattat tacttaagtt
    22501 aggatcgatg tctgttacgt aatcgacgta tgtttgatca atcttattcg tgtgctgtgc
    22561 tttatcttct tcgctgactt ttgcagtaat cgttttgctg tctggataat ccgctttgct
    22621 tgcacccaat ccggcatcgt cacgagtctg gttagtcgtt accttagaaa tagtaatcgg
    22681 aaatttcgcc atcgtttcag cttgcgtgtt atcgatttgt ttttggaatc cagaagaaag
    22741 ggacagaacg atcgcgattc cgatgatccc aatactggaa gcaaaggagg tcaagaaagt
    22801 acgtcctttt tttgttcgga tattgttgaa ggaaagcttt aacgctgtcc aaaaactcat
    22861 ctttgttctg cgtaagttaa attgatcatc ttttggtcgt tcgatgtgag gatgggaatc
    22921 tgttaagatc ttaccatcag aaaattcaat gattcgatct gcgtattgat gggccagttc
    22981 aggattatgt gtgaccatga tcactaattt ttctttcgat aactcttgga tcaattccat
    23041 gatctggata cttgtttctg tatctaacgc tccggttggt tcatcgcaca aaaggatatc
    23101 tgggtcattg gctagtgctc gagcgatggc cactcgctgc atttgtccac cagataactg
    23161 atttggcttt ttatgcatat gatttgtcaa gccgactcgg tgcaatgcat cttctgcccg
    23221 ttttcgtttt tcgtctttag aaacaccact taatgtcatc ccaagttcaa cgttttcaat
    23281 aatgcctaga tgaccaatca agttgtaact ttgaaaaatg aaaccgatag agttgtttcg
    23341 gtaggcatcc cagtcactgt ccttaaagtc tttagtagac tttccgttga ttaccatatc
    23401 accggaatca taattatcta gtccaccaat cacattcagc atagtcgttt taccagaacc
    23461 actaggacct aaaatcgcta cgaattcttt ttgtctgaaa gcgacagaca caccatccaa
    23521 tgctttggtg gtggtttcgc cgactttata atattttttt atatctttta attgaagcat
    23581 aatcaccctt ctcccgtatt gaaatattcg gtatactaat aagtatatca gaacaatgat
    23641 cgtagtatac gaaataaatg ttaactgaag atgaactaca agaaaaaatc tagaaatttt
    23701 tcaaaaaaca gagggagatc aatgaatcgt attttagtag tagaagatga tccgcaatta
    23761 cagttgcttt accgttctgt gttggaacga gcgggctttt cagtcatcac cgcattgaat
    23821 ggagaggatg cgctgaaaaa actagaagcg acacgtatag aattaatcat tacagatatc
    23881 atgatgccga aaatggatgg ctatgacttg ctagactcat tacgtagcac cagatttgac
    23941 acacctgttc taatgattac ggcaaaggcc gattttgagg ataagaaacg cgggttcaaa
    24001 ttaggagcag atgattatat gacaaaaccc atcgatgtca atgagatgat cctacgtgtg
    24061 gaagcattgc ttcgacgttc aaagatcaat tacagcagta gcttgataat cggacaaaca
    24121 aaattagagc tagatgctta tacagtgtta caagatcagc attcgttgat tctgcctcaa
    24181 aaagaatttc tgcttctgta taaattactg tcttatccca acaagatctt cactcgccaa
    24241 cagttgatgg atgatatttg gggattagat acgaatacag aagaacgaac gatcgatgta
    24301 catatcaaac gtttaaggac ccgttttgaa aagacggaag attttcaaat catcacagta
    24361 cggggactag gctataaggc ggttatttaa agatgaaaaa aaagctattt tcacaattat
    24421 ggatctattt tgctagtatt gtcttcattt cgatcttagt gacactgctg tgttttctcg
    24481 gttttatttt cttattgtct cgacattcta tctcgcctgc tgaatcggct ggaaaaccaa
    24541 cgttataccc gttattcgtc tttgcggggt tcagtatggt cgtgggtaca ggaatatcga
    24601 tttttgttgg gcggcgtatc ctgcatccaa tcagtgcgct agggacaaat atgagtctgg
    24661 tagcaacggg agatttttcg attcgaatgg atgaacagca gaaagtagca gaagtacagc
    24721 aactgtataa agatttcaac gttatggtcc aagaactcaa tagtatcgag acactgagga
    24781 atgactttgt gtccagtgta tcccatgaat tcaaaacacc actagcaaca atccaaggat
    24841 atgttcaatt gttacaagcc ccaaatctct ctgacgaaga acgccaaata tttttatatc
    24901 ggattatcga aagtatcacg cagctttcac aattaacaga gaacatctta aaattgaaca
    24961 aactggaaaa ccagcgtatc caactggaaa agaaagagta tcgattagat gaacaaatca
    25021 gagaagtaat cgtttttttt cagcctaaat gggagaaaga acagctagaa ttggatatcg
    25081 aacttgctgc agtaaattac acaggcaatg aagagttttt gtaccaagtg tggctgaata
    25141 tcatggataa cgccataaag tacaatcaaa tcaatggaca gattcatatc aaactatttg
    25201 agacagccac agagattgta ctggaagtga ccgattcagg tgtaggaatg aatgaagaga
    25261 ccagagatcg aatgtttgaa aaattttatc aaggagatac aagtcgtcaa atttctggga
    25321 atgggctagg actttcactg gttaaaaaaa ttctagaact gcatgatgga cgtattgatt
    25381 atagcagtat cgaaggagta ggaacgacag caatgatacg gttaagtaaa caataggaaa
    25441 aagaataaaa cccctaaaaa ttctattgaa ggtttttagg ggtttttagt cgtttttttg
    25501 tgattaggga aatgttctta cggttctttt ggcataaaaa tcgctgcttt cttattgaac
    25561 tacgagaaat gttagaggtg tcattgaaac aaggacatga tgcaagaaag agagcgaaag
    25621 tcatgcgtcg ctctcttttt ttgggtggat agatggacag aaaatgtagg gcttagtttt
    25681 cttcttgatt taaatactcc caaaatgtat ctgaagccaa agaatgctca ccgactttac
    25741 gagtaaatgc aagataactc tctgttgttg gagaaatagg gagaatcaca gtattttctt
    25801 ttaatagatt cttgactgtt ttttccataa caatagcaat ccccatgtca tttgcaatca
    25861 tattgatgat caagtcgatt cttgctcctt tataagagat ttttggttca aatccagctt
    25921 cgcgacacag atctagtaca ggttgataca agttcgtagt ggggccaagg agcaaaaagt
    25981 tctccttttt cagttcgctt agatccaatt tctttttact agctaaagga tgatttttag
    26041 gtagaactgc tacaaaatcg tctgtttcag taggcaacca ctcaatcgaa cttggtgggg
    26101 cctggaaact tctagcaaag tggatcgtgt cactatgttc attggcgtca ttgtaagtta
    26161 attcgacact ttccacttct ttcaactgga ctgtaaattc aggatggcgt ttcaaaaact
    26221 cggtgatttt ggaaaaacct ttataattga tcattgtcgg tatcgttaag atatttaatg
    26281 aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg tattgttcta
    26341 cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg atttttctat
    26401 gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc tgtttagaaa
    26461 tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt gtttcggcta
    26521 aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt attccaataa
    26581 agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta caatctttct
    26641 tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt agaaaaagag
    26701 aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt gtttggcgga
    26761 ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca gctgacgatg
    26821 gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat gaaaaatatg
    26881 ccggctgtta tttttgcttt agtgatagga acagcgattg gtttgattgt tcatttaggt
    26941 aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat cttccctagt
    27001 gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt cattgttcta
    27061 ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac aggagacagt
    27121 acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt tgcgtgtaat
    27181 ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta tattttgttc
    27241 ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga ttttaaagct
    27301 tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa aatgttccca
    27361 gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat gtggacaaat
    27421 tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct gactaaaaag
    27481 gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa ctatattata
    27541 tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt gacgtaatgc
    27601 acatccagat tccaactgtt cttcttgatg gtactgtgtt agcagcatta agcaaggaag
    27661 atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc agtgaatcta
    27721 cactatatcc ggttttgaga aggttgaaaa aagaaggata tcttgagacc tatgatgaac
    27781 cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt cgtcatttag
    27841 aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta ttggaggaga
    27901 gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat tcctgaagac
    27961 gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg aaaaacatta
    28021 gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt aaaaatcagt
    28081 tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa aagacaaatt
    28141 cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat tccactagca
    28201 ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct catttttagt
    28261 atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat ctcaggaatc
    28321 agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg tctgttcgcc
    28381 acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg gcttttttat
    28441 gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag agatctgcat
    28501 tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt gattaccggt
    28561 attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt aggcgctcaa
    28621 aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga gacaaaaaaa
    28681 atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc ggtcatacgc
    28741 ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga aataaaggag
    28801 aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat cattagttta
    28861 gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt atctgaattg
    28921 acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa aaaagtgaat
    28981 actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac aataactgcg
    29041 aaagatgaat taggctcact tcgactagat gaggccaatg tggataactt gatcgtcaca
    29101 ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg aaaagtcatc
    29161 ctaaacggtg gtaatttatc tttgcatgag atggaaaaca gtggctatac tatccaaacg
    29221 tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt gttagaacaa
    29281 ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac gacggatcaa
    29341 gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa aaacagcaaa
    29401 gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag taaaataaga
    29461 aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa gaaggtcaaa
    29521 gagaaaagtt aaagacagaa atggaaagat tacatactta tatcactcaa ctaagccaaa
    29581 ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat tcagaaggtc
    29641 gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg aagaaagagc
    29701 tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat gggaagaagg
    29761 aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg atggatctag
    29821 aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca aaattggatt
    29881 atttaccgca ggcagccgga gggacacagg caagcggttg gtacatcact gaagacaaag
    29941 agttagaact agaaggtgta atggcgcgca ttcgaaaaaa aattaaaaaa gtaacattag
    30001 atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag agcattttat
    30061 atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg attgtttttc
    30121 ttctatttag gcatgtggca agcgaaagtt tgggcacttt ttttaagtat ttttctttgg
    30181 ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca agcaaaagta
    30241 attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt ccttacgata
    30301 cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag ttctgtttta
    30361 aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt ctttaaagtc
    30421 gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt agaaatccaa
    30481 agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt caatgagaat
    30541 gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa agagctgcaa
    30601 gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca tttagcctat
    30661 gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat tttgtctgct
    30721 cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga gcaaatcgaa
    30781 gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa taatttactt
    30841 gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga catgaaagac
    30901 cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat tattgtcttg
    30961 aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat gatagttgat
    31021 tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga gagaagggga
    31081 aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac ttgcaggggt
    31141 gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa ttacgacaga
    31201 tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact atagatatat
    31261 cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg gacaattgac
    31321 tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata aaggtgtcac
    31381 ttcgtatgaa gaagtcgaaa aaaaagacgt tccgaaaaca gcactggaaa aactaattaa
    31441 agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa agtatacgga
    31501 aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt agaaaaaggc
    31561 gaatttattg ggatcatggg cgcttctggt tctggtaaat cgacactttt gaatatttta
    31621 tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga tgtgaccaca
    31681 ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat tttccaagac
    31741 ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct ttctttacaa
    31801 ggcgtcaaac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg tttatccatc
    31861 tctcatattt tggaaagcta tccttctgag atttctggtg gacaaaaaca acgggtcgcc
    31921 gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc tacaggtgcg
    31981 ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa tacaaaagat
    32041 catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg ccaacgtatt
    32101 ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca aagccgtgat
    32161 tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg ggagattgac
    32221 tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact acctggtgtt
    32281 attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa tggcaagcaa
    32341 cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt tccgattcgg
    32401 ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt cctttttgat
    32461 gacgatgcgg caaaaagact atgcaatgtt tatgatgcta ggagcaaaag gcagaaaaat
    32521 cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt tagtgggttc
    32581 agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc agttagatct
    32641 caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg ttttgttttt
    32701 tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca aaaaaccgat
    32761 attagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata aattcttgtt
    32821 tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata tgatgaaaca
    32881 cgtggggacc tatcaagtct ttggtatagg agtagcctta gttacgatcg tattaggaac
    32941 ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga aatcagaaca
    33001 gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc gtattcgtga
    33061 atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg gcgctttgac
    33121 ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt ctggatatga
    33181 tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac tttcaccaac
    33241 actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata atgcaagtga
    33301 atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc cgaagatcac
    33361 gaaagaaaaa gttcctgctg aaaaaatgaa tgaactgact gtacaagagg aacttcgaag
    33421 ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat cagaatttga
    33481 tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact tccaagcaaa
    33541 cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat ctttgcaaaa
    33601 agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc tattctctgg
    33661 ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg ctagctgttt
    33721 gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa tgcttgaaaa
    33781 gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag gcgtgttatt
    33841 cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa tgtttggtct
    33901 attcatgcaa aatccataca aagatatttg ggttccgttt acgatcttct tcgtattgta
    33961 tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga aaaagagatt
    34021 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag ctcctcctat
    34081 ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttgttcggaa
    34141 aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt attagaatat
    34201 agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt ccatgtatac
    34261 tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca atgaacaaaa
    34321 ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat ctttgtctgt
    34381 cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg aaaaacgaga
    34441 gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt atatcggtgc
    34501 ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag atgatgaaac
    34561 gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg cacctagctt
    34621 tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat cggtcatttt
    34681 ctatcagact agcccagaca tggaaaacgg atttcctgga actttagatg tagaagtacg
    34741 gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca gtgaccgcaa
    34801 aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc cagcacagtc
    34861 aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca ataaagatac
    34921 aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc aacgtcctaa
    34981 aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg gcggtatcga
    35041 tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat tgacaagccc
    35101 agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga tttttacagc
    35161 aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc atggcggtat
    35221 cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg gagatgtcac
    35281 tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata taaagaaata
    35341 gaataaatta aaaagggagt gtgacagtgt cacatcccct tttta
//
LOCUS       DACDNO010000024        34350 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDNO010000024 DACDNO010000000
VERSION     DACDNO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.024"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IWI22_001243"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IWI22_001243"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP9168644.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IWI22_001244"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IWI22_001244"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP9168645.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IWI22_001245"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IWI22_001245"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP9168646.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IWI22_001246"
     CDS             complement(4028..5044)
                     /locus_tag="IWI22_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9168647.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="IWI22_001247"
     CDS             complement(5146..5343)
                     /locus_tag="IWI22_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168648.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="IWI22_001248"
     CDS             complement(5315..6634)
                     /locus_tag="IWI22_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP9168649.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7375..8355)
                     /locus_tag="IWI22_001249"
     CDS             complement(7375..8355)
                     /locus_tag="IWI22_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9168650.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="IWI22_001250"
     CDS             complement(8368..9246)
                     /locus_tag="IWI22_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9168651.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="IWI22_001251"
     CDS             complement(9262..10062)
                     /locus_tag="IWI22_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP9168652.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            11454..11780
                     /locus_tag="IWI22_001252"
     CDS             11454..11780
                     /locus_tag="IWI22_001252"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9168653.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11788..12696
                     /locus_tag="IWI22_001253"
     CDS             11788..12696
                     /locus_tag="IWI22_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9168654.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13325..13576
                     /locus_tag="IWI22_001254"
     CDS             13325..13576
                     /locus_tag="IWI22_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP9168655.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            14118..14276
                     /locus_tag="IWI22_001255"
     CDS             14118..14276
                     /locus_tag="IWI22_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9168656.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            14348..15574
                     /locus_tag="IWI22_001256"
     CDS             14348..15574
                     /locus_tag="IWI22_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9168657.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="IWI22_001257"
     CDS             complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="IWI22_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9168658.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="IWI22_001258"
     CDS             complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="IWI22_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9168659.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(17033..17500)
                     /locus_tag="IWI22_001259"
     CDS             complement(17033..17500)
                     /locus_tag="IWI22_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9168660.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(17620..19158)
                     /locus_tag="IWI22_001260"
     CDS             complement(17620..19158)
                     /locus_tag="IWI22_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9168661.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            19238..19924
                     /locus_tag="IWI22_001261"
     CDS             19238..19924
                     /locus_tag="IWI22_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9168662.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(20125..20652)
                     /gene="msrA"
                     /locus_tag="IWI22_001262"
     CDS             complement(20125..20652)
                     /gene="msrA"
                     /locus_tag="IWI22_001262"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9168663.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(20736..21413)
                     /gene="pgmB"
                     /locus_tag="IWI22_001263"
     CDS             complement(20736..21413)
                     /gene="pgmB"
                     /locus_tag="IWI22_001263"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9168664.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(21410..22540)
                     /locus_tag="IWI22_001264"
     CDS             complement(21410..22540)
                     /locus_tag="IWI22_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9168665.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(22540..24723)
                     /locus_tag="IWI22_001265"
     CDS             complement(22540..24723)
                     /locus_tag="IWI22_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9168666.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(24720..25745)
                     /locus_tag="IWI22_001266"
     CDS             complement(24720..25745)
                     /locus_tag="IWI22_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9168667.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(25790..26617)
                     /locus_tag="IWI22_001267"
     CDS             complement(25790..26617)
                     /locus_tag="IWI22_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9168668.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(26614..27681)
                     /locus_tag="IWI22_001268"
     CDS             complement(26614..27681)
                     /locus_tag="IWI22_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9168669.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(27707..28543)
                     /locus_tag="IWI22_001269"
     CDS             complement(27707..28543)
                     /locus_tag="IWI22_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9168670.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(28540..29409)
                     /locus_tag="IWI22_001270"
     CDS             complement(28540..29409)
                     /locus_tag="IWI22_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9168671.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(29479..30762)
                     /locus_tag="IWI22_001271"
     CDS             complement(29479..30762)
                     /locus_tag="IWI22_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9168672.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(30776..32461)
                     /locus_tag="IWI22_001272"
     CDS             complement(30776..32461)
                     /locus_tag="IWI22_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9168673.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            32736..33761
                     /locus_tag="IWI22_001273"
     CDS             32736..33761
                     /locus_tag="IWI22_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9168674.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         9804 a         7370 c         6045 g        11131 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac attctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag ctactacggt aacaactata aattcttttt gattatctgt caagagttag
    10141 ttcaataaca cacatataat aattagctta aaaacaatct ttttattaaa aagaaacatt
    10201 ataatatgaa tgtttgtgaa aaaataatac aacttagtca ttctttacaa cgatttatag
    10261 aaaaacaacc tctttaaata taaaaggtac gggttactga ttacttgtaa atctttacgt
    10321 tccagtttta ttctttctaa aaaatatact cacttctttt cttagacttc tttttttgat
    10381 tctcctcttt atggtcttgt ccttttttgc cttcaaaata tttaaataaa cccctttcaa
    10441 aagcatttct aggttttgga caataaggaa tgactttttt cttcatataa atacctcatt
    10501 taactatttg atatcatcac attatcaaaa aaaataggtt ttttattaaa actatcttga
    10561 cctttcggga gatttcactg tgatttattt ttattcccat acttttttcg ttggtcactt
    10621 tcgtatacct aatctaagcg atcatcggcg acggtgcttt tacaacgaat ggcaggacaa
    10681 aacaaggggc ggaaaaactt ttttgcctat ttcataaaaa actttttcct acagcaatcc
    10741 cttgttttgt cctaccacac gttgtgaacc caaagcacgc cgacgaacgc ttagctatac
    10801 gaaaatgacc tccaaaaaca aaaaagtgag gtgaatagaa acaaaaagaa attgaaatct
    10861 cccaaaatgt cgtccgcctc ggagacaact tgctgctgaa aatggttggt taattcattt
    10921 aagtattaaa taagataaat catcaaatta atcggaaaaa tagcatgatt ggccttatca
    10981 aaaataaact gtaaaaactt atatttctat gagtggtgga acaatgctaa taccaaatta
    11041 atttatcgat tacccacctg cacaaaccaa agatagcatt attaggaaac gaatcaatca
    11101 gcgaatccag cattataatg aggatactaa aaataaagtt ttcataatta taaatccgcc
    11161 ttcctacgaa aggcaccaat gaggtgatga aaaccttaga gcgctttctt tcttttttta
    11221 ttccgtgtta ccctttcctt gcaggtccct ggtacactac agaacccgag gaggtgagtg
    11281 gcggggctgt atagcggcaa ccccgcataa ttatatggtg tcggtcatcc gttaaggcat
    11341 caatgaatgg cgcaaacccg tagcccgtaa acttatctct tccgaagttg actcgatttc
    11401 gccggatgac cagtccctac caaacctgta ctattttttt acaggaggtt gctatgttca
    11461 aaatctttcg taaaaattgt tgtggcttgg atgtccacaa aacgtggatc tatgcgtgta
    11521 tcggaatcac agatgcgaat ggtcgtacag tgtataagca agctcgcttc tcttccttta
    11581 caaaaggtct caatgaatta gctgaatggc ttgctaaata caactgtaag gatgtgtgtt
    11641 tggaatcttc cggtaaattt tggattcctg tttttaatat tcttgaaaaa acttgttttg
    11701 tcacactggc tcatccgaag tacaccaaac cccagaaagg aaataaaaca gatcgcaaag
    11761 atgcaaaaat ggatttgtga tctctttatg tgcgacatga tcaaacccag ttttattcct
    11821 ggtcttgaaa tccgccagct tcgtgatcta atgcgttatc ggacgaaact gaccaacatg
    11881 aaaacaagtg aaaagaatcg tgctttaaac tgcttaaccg tttctaatct gaaactagat
    11941 gatgttttta gtgatgtgtt cggaaaatca tccacttcta tcatccaata cttgttagaa
    12001 catcccggtg aatcatttga tgttcgacca tttatccatg cccgttgtaa aactcccgtt
    12061 gaagaaatcc aagcggcagt tgagggggcg atttctgtac ctcaagcgat taagcttcgc
    12121 caatgcctca accatattaa cgagattgag aagcatcgag aagagatcaa tctagagatt
    12181 caagctcttg cagaaccctt ctcccctgtt ttggaactac ttcgtaccat accgggctta
    12241 gacactcagc caataacagc gattgcgatt ctttcagaaa taggaccgga tatgtccgtg
    12301 tttccaactt cgaaacactt tatttcttgg gctggatgct gtccaagaaa cgatcgcagt
    12361 gcccgacgaa caaaatcaac acgtatctct cgtgcagggc agtcgttgaa accgctactt
    12421 gtacagattg ccaatgctct tctcagatcc aaagaacatc cagaatttaa aaatcgctat
    12481 cgtagaatca aagcgaatcg tggacacaag aaagctatca ttgctatttg taagatgctc
    12541 ctgaccgcta tctggaatgt cttatcaaag gtggaaccct attctgctaa aggatatctg
    12601 gagccaaaac ctgcgaaaga agagaaagtt ctgacgcaat cccaagctct ggacctttta
    12661 agaaaacgcg gatataccat tatagatagt gcttaaacca aagttacgtt gattcttcct
    12721 aaatagactc gttgtcttcc atttcaaaaa ttttgaaaac ggtaggctta tttagtgtac
    12781 cgttcttcag gtatcttcaa atagatttat tttcaaactt atttcctttc ttcttttact
    12841 ctcctatagc ttagcattta agtgggcact cgtgtcacct tgctagatag ctatgaatgc
    12901 cgtaataagc ctcctagtat ataggcagac tgccaattat aactgattca atccacatta
    12961 tgataaaatc caccttttcg tttcatatgt gttgaaatat cacatatgtc aaaagtgcac
    13021 gagtcttttc attcagtgct ttgtaaatac tcaaaattat tccttttcac tttaaaaaaa
    13081 agataattag tacaaaatca gaaatgagga aagtattagg atggactaga aaggagttgt
    13141 tgaatttgtt gcaacagatt ggtcatttat caattaaaaa ttaggtgaag ttttattcat
    13201 cactgcagct gctgtttccc agagaataaa acgtctagaa gaaactggtc agttactgta
    13261 ttatcaagca gcaattgact acgaacaagc ttgattacta accaaagcgt ttatccacct
    13321 gttgattaaa tcaaatcaaa aatcaggttt ttatacgttc ataaaagcaa tctccagtgt
    13381 actcgaatgt gactgtatca taggtcctta ttcgatgtta atcaaaatgg ggtttccttc
    13441 taccgaatca ttggacagtt ttatcaatga tttatcaaag tttggacgaa ccaacaccca
    13501 aatcgttttc tcgactcttg tttccttaat ggtttatcag ttctggaatg tttatcatgc
    13561 gcttttatta aaatagaaaa gagctttttt catgcgacaa agcagtgatc atttattggc
    13621 acatgcaatc tttgtggatc cattttttga aaatacgata tttagttttg acccaaaaat
    13681 ctttaaatac gtatttgata atttatctta acaaccagat aattttattc agcaccgtat
    13741 tatctatatt gaattctcgg aatcaagata aatttttttc cgtttttgtt tcttttttaa
    13801 tcttcttctt gttattaata gtgattagga tgatattcct atattgatct ttgataatta
    13861 gatataattt ttgaataacg ttccctgcta ttcgagctag aaatacaagt acgccatgac
    13921 ctagttagcg acaactactt agagatttct tttgttctgg gacaacaaaa tgccatgaca
    13981 atagcagtaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc
    14041 ctatgtgctt acccaaagcg gttcaaaaat tttaaactat tattttagga gatgatttta
    14101 taaaatcgaa agcctacttg aataaaaaac aagcctgtga atttctagga attgataata
    14161 atactttaga taagtggatt cgtgtatata attttccagt aataaaaatt gatggcgtag
    14221 ttcgctttga cgtagaggat atcaaagatt tcatgaatac attcaaaaaa aattgaaatt
    14281 ttcttctcgt cacatatact ataaatagcg aaatgtactg ccttactttt taaaggagtg
    14341 aggaagtttg cctacaaagc tttcaaatgg gaaatataaa actaatctga ggtatccaaa
    14401 aaaattcaga gaaatcactg gaattgcatc agagaagtat caaaaagtat ttccaactag
    14461 gcaattagct attaaggccg aaagggaaat gaaaaagaag attgaaacag ttttaagaga
    14521 ggagaatgca aactcactag aattgaaagg aaaaataaaa tttaaagatt tttatgaatc
    14581 aaaatggctt cctagatatg aactaggtca aactactagg tcaaacaggg ctccaagtaa
    14641 tgtaacaatt agtaatacta aggatatatt ccgattgcac atccttccaa tgtttggtga
    14701 atatgcaatg aattatctga attcaaatac tgagataata tcagatgaat taacaaagaa
    14761 atcgaaagaa tacgcaaata taaaaattat caaaggctat gttcgctcta tgtttgatat
    14821 tgccgaaata ctatgttata tcgaattcaa ccgaactacc aaaattattc aatgtattac
    14881 cgcgcctaag aaaatggctt tagaagaaaa aaggataagg gaagggaatc aagcattatc
    14941 atcagaagag ctgactgatt ggatagaagc agtaaacaat gacttaaata atcacttgct
    15001 tacacttcac gattacacac ttttcatgct taccttatat ctaggtgata gaaaaagtga
    15061 gacttatgct ctgcaatgga aatatattga ttttaataaa aatacggttc gtctcaaata
    15121 tgctttagat aaatatcaaa gaaagacaca tacaaaaggt tggaaagaca ccgttattca
    15181 ggcaccagaa gttgtgatga cattattaag agaatggaaa tcagctcagg ctgaacaatt
    15241 gttgaaacta aaaattatac aaacacctga ccagtacctc tttacctatt ccaaaccttc
    15301 tggagaagta aattgtcctg ttcacgctga ctacttaaac tatcgaatca atgcaataaa
    15361 aaaaaggcat cctgaattgg ctcaattatc tccacacaaa cttcgtcata cctatgcaac
    15421 tatagctagg gaaggtggag caaatatgaa tcaaatctct aatgctttaa cgcactcgga
    15481 tatttctact acaaaaatat acgtgaacac acctgacgta gttgataaat ctgtttttga
    15541 agcatttcaa aaaggattga ataagtgtga ttaacatgtg ttctcaaatg tgttcttatt
    15601 ataaaaaaag acactttcca aataattgaa aagtgtcctg aaatattgat ataaaaagaa
    15661 tattaacgtt ttgagaattg tgaagcttta cgagcttttt taagacctgg ttttttacgt
    15721 tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca
    15781 ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg
    15841 tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg
    15901 aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt
    15961 ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta
    16021 cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag
    16081 tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat
    16141 acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca
    16201 gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc
    16261 aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt
    16321 aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg
    16381 aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat
    16441 ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata
    16501 tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga
    16561 gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt
    16621 caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt
    16681 cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa
    16741 gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca
    16801 agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga
    16861 gctgaacggc aggcttacaa cctcttggta aacggtgttc aatcagctga ccctcggtat
    16921 tagctcaaaa aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag
    16981 gtcaaaacgc tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt
    17041 cgatctctgg cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg
    17101 ttttgaattc gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga
    17161 ttcctttttg gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt
    17221 ctggaatagt acgatcccag cggccaattt catcagggat ttttgtgtta tctgcaaatt
    17281 cacgcaatgt agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta
    17341 actgagcgtt caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg
    17401 gtcctctcat gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa
    17461 ctaattgatt taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt
    17521 ttcttacact taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg
    17581 agaacgattt tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa
    17641 ttcttctcgc agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac
    17701 aaataatgga tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc
    17761 tttcaattct atcaattcct gtgcttcact gatactttgg atctttccga tgcttctcca
    17821 gcctaaaatc acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta
    17881 acccagtaca tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc
    17941 acggatctct gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag
    18001 acagatccct tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc
    18061 gataaaactg ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg
    18121 atgaaaaatc agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc
    18181 atatggattt tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc
    18241 tgcccaatca aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg
    18301 atccagaaca taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa
    18361 acgaagtgct ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa
    18421 ccctagatta gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa
    18481 acctagttct gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac
    18541 ttgcggcaat tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa
    18601 ttcatggata tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc
    18661 aaacaatcta taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa
    18721 ttcaggttgg ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg
    18781 cgtcccagtc tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc
    18841 gtccgctgct cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc
    18901 caacaatatc aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga
    18961 aggcaactgt ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg
    19021 atcttgttct gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc
    19081 tgcttcttgg gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa
    19141 tccgattgaa acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta
    19201 agcttgcgta tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg
    19261 ttgctgtatc ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat
    19321 tatttatcct agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat
    19381 tccgattctc tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc
    19441 cttctactat ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt
    19501 ttcttccgca agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt
    19561 gatggattta ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt
    19621 atggattttt tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat
    19681 cgtaggcggt gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt
    19741 gatgctttct tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc
    19801 tagtctgttt ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga
    19861 aaagctgcct ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta
    19921 ttgaaaaaag gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc
    19981 aaatccttgt gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca
    20041 atcagctgac cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt
    20101 tccataagta gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc
    20161 tgcacgtcca gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc
    20221 ctgatggtaa tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg
    20281 cttatcgaat cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc
    20341 ttcttcatca ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc
    20401 taatgcgtca gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat
    20461 aacttctgga tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac
    20521 ttgttcataa gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat
    20581 ccctggctgc tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc
    20641 tttttttgtc atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga
    20701 taacgttttg cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat
    20761 aaatgccaaa acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca
    20821 gaaggaagat ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct
    20881 tgacaaccag cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc
    20941 tgtgctcctt tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca
    21001 ataaggacat caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg
    21061 cttgctgatc ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt
    21121 gcaccaggca aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt
    21181 ttttgctcag caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat
    21241 gacagcaatc gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc
    21301 tcagtgaatg gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga
    21361 gctgtgtcta caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac
    21421 ctccttgaaa atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat
    21481 tatcagtggc ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa
    21541 cggccgaaat gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag
    21601 ctatcacagg aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta
    21661 gactttgtcc atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt
    21721 tatcgaactc cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa
    21781 atgctaacag tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt
    21841 gaggggcaaa tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt
    21901 ctttcaagcc tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat
    21961 tagtaacatc agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt
    22021 tatcaactgt agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt
    22081 gatcgtaaaa agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac
    22141 ttccgccaac gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt
    22201 ctcctactcc tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca
    22261 atccgacgat ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt
    22321 ctttgttttt gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt
    22381 ctgctaatga tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag
    22441 cctgaacaaa aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca
    22501 gactttcttt gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa
    22561 gatcagttct ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt
    22621 cgtctctttt tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt
    22681 ccaaataaaa ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag
    22741 agttccattc gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc
    22801 taatgaagca gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag
    22861 gcacgcttct tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat
    22921 ggcgtgaatc gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa
    22981 attcttagaa acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag
    23041 atctgcttgc ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat
    23101 agactggctg ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata
    23161 cgtgtcatct tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa
    23221 aaaattttga cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc
    23281 cacattataa tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc
    23341 atctggacca atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag
    23401 ccaaaaagct gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc
    23461 ctctgttgct tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc
    23521 tgcggcagct gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt
    23581 gggtgtctct tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg
    23641 ataatctctt gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt
    23701 ctttgctact tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca
    23761 gaaaacatgc cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg
    23821 atcattgcgg ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc
    23881 gaaagaagaa tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga
    23941 catcgactgc tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt
    24001 cttaaacggg tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa
    24061 ggacgtattt tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg
    24121 gaatacacct gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt
    24181 ctggtaggat gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg
    24241 ctgtgtacca aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc
    24301 ttcggtcaac ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc
    24361 aacacgacaa gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg
    24421 tttaagctct cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc
    24481 aagagaaaaa tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac
    24541 taactcggct gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct
    24601 tacttgattc cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat
    24661 ggtcatctga ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt
    24721 cataaggtaa taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg
    24781 atgacatatc cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc
    24841 acaaaatgag ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa
    24901 accagctttc catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga
    24961 tcgccacaaa aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc
    25021 agttggatga tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg
    25081 aatttgcttg gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg
    25141 aagctgtacg cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca
    25201 agcatgtgaa tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac
    25261 gttccccagc ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc
    25321 ccgagaacat caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat
    25381 ccataagcca aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca
    25441 gctgtcatag ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat
    25501 acatgttcga agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca
    25561 ttcagcattt gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct
    25621 tctgctcgct ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt
    25681 tgataataag cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc
    25741 ttcatttatt tctctctcct ttgcttattt acttattgtt attctttctc tattctttct
    25801 ctaaatcatt taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct
    25861 gataaatttt cacttcattt tctcctcgca aacgacattc atataccata taaccttcat
    25921 actcattttg aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt
    25981 ggtagcgatg attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg
    26041 cttttgaaat atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat
    26101 gtttcaattg attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt
    26161 cttggtatcg gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat
    26221 ctaaataacg aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa
    26281 ccatcggcgg taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc
    26341 ctccaacctc gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc
    26401 gttcttcgat aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga
    26461 tccctgtttc tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt
    26521 cgatttcgta ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc
    26581 ttgggaaaaa tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac
    26641 gcccattttg atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct
    26701 ttctgcaaat ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa
    26761 taattcccaa catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg
    26821 ttcactagat gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag
    26881 attaaaccca gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc
    26941 gatccctatt aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc
    27001 tactccacgt ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc
    27061 tagcgtgtaa tcggcaccgt gtttttctgc taattctcta cgctgcgcta tgggatcgat
    27121 cccaacgatg gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc
    27181 gcctaaaccg aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc
    27241 taaagcgaat tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag
    27301 tttacgtagt ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata
    27361 agtgcagact aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt
    27421 accgacaatc atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc
    27481 ttctcttggc aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa
    27541 tcctctaaag tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg
    27601 tacttcgttc ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc
    27661 taatgcaact aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc
    27721 cgccttcgac taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg
    27781 gtaaagcggt caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga
    27841 ataatgtgtt caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag
    27901 caaataaata atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag
    27961 aaatagccaa tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca
    28021 ttgctgcttc ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg
    28081 cagtaggaag tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac
    28141 ctagaccgga aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa
    28201 cggtgtaaaa gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt
    28261 atgccccgct gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac
    28321 tatttttgaa atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt
    28381 caaaagtcca ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct
    28441 tgaatgatgt catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa
    28501 tcgtcgcgta aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt
    28561 tcgtgagagc aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc
    28621 ttttccaaga tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac
    28681 tttattcgtc agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac
    28741 aatcacagtt gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca
    28801 agcatttgca ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat
    28861 agccaaaaat gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat
    28921 aaagaagcta gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct
    28981 atctactaac agatcattga cgattccata actattattg aacatatatt tccaagcaaa
    29041 aatcaaagaa atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct
    29101 aaatctgaac ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc
    29161 tacaactaag aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact
    29221 taaaatttca agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg
    29281 catatcgaaa aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa
    29341 taaaagcatg cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg
    29401 acgattcatt gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac
    29461 cattggcaga acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac
    29521 atcttcgttt ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat
    29581 atctcccatt ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa
    29641 agcactagca atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta
    29701 accatccagt tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac
    29761 ccacttttcc atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt
    29821 ttgtttctcg tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg
    29881 atctttttgt gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga
    29941 aggcaaaatg taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt
    30001 gatttctgtc acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga
    30061 caatgcttct ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt
    30121 tgctccattt gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt
    30181 aggcatagca attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa
    30241 aatatcatcc caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata
    30301 ccaaatacct tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga
    30361 acgaacaagg tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt
    30421 ttggactgcc gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact
    30481 gacttccatt acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa
    30541 cgctgattct tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc
    30601 agcaaccagt tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg
    30661 aatcgttgtc tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa
    30721 tccagtagct agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt
    30781 ttattatttt cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc
    30841 aatggataat ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg
    30901 ctgattgatt ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa
    30961 aactgataaa agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt
    31021 tttctttttt tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg
    31081 gatctgtctg caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc
    31141 gattctctcc tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa
    31201 tataaattgc cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt
    31261 tgcttctcca ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta
    31321 tctcatatgg actggtcgtt ccatctgggt ttttcttgta gtttactcgg gcaccctctt
    31381 tcttcagctg ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat
    31441 tcaatccgat accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga
    31501 aatcgatggt ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa
    31561 gggtcaaagg tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa
    31621 aataactgat attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag
    31681 ctgcttcatc agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta
    31741 tacaagaagt tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag
    31801 cgcctttttc taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt
    31861 taaaattcaa gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt
    31921 ttttgtcaat gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat
    31981 ctgtatccag tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt
    32041 cctgaaacca agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg
    32101 tttcgcttct catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat
    32161 aatttacaac ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa
    32221 ctctaaatgt atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt
    32281 gctgctcttc aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat
    32341 gcagtttgcc tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag
    32401 aggcggtttt ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca
    32461 tcggattccc ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg
    32521 cttactgtca atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa
    32581 gcttatgtat caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag
    32641 ctattcctaa attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat
    32701 tctaaaacgc tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg
    32761 cgaaaaaagc taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata
    32821 tctctgataa agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga
    32881 gcaaaattgc aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc
    32941 gtacttctca tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg
    33001 ccgtttctga acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag
    33061 agctagaaaa atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa
    33121 gctctacgat ggatgaaaca cttctttttg aattggatca atacaatatt cctattgtgg
    33181 taatcggaaa agtcggaaac gactacacga acatctactc agtggacacg gataatttcc
    33241 aggatagtta cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc
    33301 tccatgctcc gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct
    33361 tatttgatca ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag
    33421 aagatagcat ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt
    33481 tttctaccga tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc
    33541 agatacctga tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt
    33601 cgccacagtt gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac
    33661 ttttattcca actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg
    33721 agattactct cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac
    33781 agaaacactg aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac
    33841 cccctcccgg tattttagcg aatttttctt attttccatc tgttttagat agctgtggat
    33901 aactataatg tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca
    33961 cgttgtgtat aagtttttat agttcgtttt tttaaacaca tttaccctta tttcaatcca
    34021 tgttatttgg ggatacttat ctctaaaaca taaatagaat aacagacatg tctactggtt
    34081 ttgttttatt cgaggaatta ttcaataata gctagcaaaa tattaaaaat aaccgctcta
    34141 tcttcccatc aatttatttt gcattcaaaa aatgatctat ctttactttt gttgattgac
    34201 ttctcttgga agatgatcgt tccgatacct taattggata cgaaccgatg ttgttaagat
    34261 cctttcccac ttttcttcta gttagacaaa ttattttaga caaaccaaaa aagaggttgt
    34321 gacaataatt tgtcacaacc tcttttttaa
//
LOCUS       DACDNO010000025        34017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDNO010000025 DACDNO010000000
VERSION     DACDNO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.025"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(87..317)
                     /locus_tag="IWI22_001274"
     CDS             complement(87..317)
                     /locus_tag="IWI22_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9168675.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(403..1290)
                     /locus_tag="IWI22_001275"
     CDS             complement(403..1290)
                     /locus_tag="IWI22_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9168676.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(1379..2602)
                     /locus_tag="IWI22_001276"
     CDS             complement(1379..2602)
                     /locus_tag="IWI22_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9168677.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(2619..3698)
                     /locus_tag="IWI22_001277"
     CDS             complement(2619..3698)
                     /locus_tag="IWI22_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9168678.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(3829..5556)
                     /gene="ptsP"
                     /locus_tag="IWI22_001278"
     CDS             complement(3829..5556)
                     /gene="ptsP"
                     /locus_tag="IWI22_001278"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9168679.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(5556..5822)
                     /locus_tag="IWI22_001279"
     CDS             complement(5556..5822)
                     /locus_tag="IWI22_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9168680.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            6301..8535
                     /locus_tag="IWI22_001280"
     CDS             6301..8535
                     /locus_tag="IWI22_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9168681.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8683..9003
                     /locus_tag="IWI22_001281"
     CDS             8683..9003
                     /locus_tag="IWI22_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9168682.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(9208..9432)
                     /locus_tag="IWI22_001282"
     CDS             complement(9208..9432)
                     /locus_tag="IWI22_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP9168683.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(9630..11210)
                     /locus_tag="IWI22_001283"
     CDS             complement(9630..11210)
                     /locus_tag="IWI22_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9168684.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(11611..12987)
                     /locus_tag="IWI22_001284"
     CDS             complement(11611..12987)
                     /locus_tag="IWI22_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9168685.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(13115..14233)
                     /locus_tag="IWI22_001285"
     CDS             complement(13115..14233)
                     /locus_tag="IWI22_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9168686.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            14375..14641
                     /locus_tag="IWI22_001286"
     CDS             14375..14641
                     /locus_tag="IWI22_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9168687.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGMPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILLKMLACLIFI"
     gene            complement(14733..15266)
                     /locus_tag="IWI22_001287"
     CDS             complement(14733..15266)
                     /locus_tag="IWI22_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9168688.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(15360..16538)
                     /gene="mutY"
                     /locus_tag="IWI22_001288"
     CDS             complement(15360..16538)
                     /gene="mutY"
                     /locus_tag="IWI22_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9168689.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(16531..17328)
                     /gene="recX"
                     /locus_tag="IWI22_001289"
     CDS             complement(16531..17328)
                     /gene="recX"
                     /locus_tag="IWI22_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9168690.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            17435..18817
                     /gene="rlmD"
                     /locus_tag="IWI22_001290"
     CDS             17435..18817
                     /gene="rlmD"
                     /locus_tag="IWI22_001290"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9168691.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(18991..19569)
                     /locus_tag="IWI22_001291"
     CDS             complement(18991..19569)
                     /locus_tag="IWI22_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9168692.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            19891..20361
                     /locus_tag="IWI22_001292"
     CDS             19891..20361
                     /locus_tag="IWI22_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9168693.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(20469..21695)
                     /locus_tag="IWI22_001293"
     CDS             complement(20469..21695)
                     /locus_tag="IWI22_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9168694.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(22449..22649)
                     /locus_tag="IWI22_001294"
     CDS             complement(22449..22649)
                     /locus_tag="IWI22_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9168695.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(22783..23391)
                     /locus_tag="IWI22_001295"
     CDS             complement(22783..23391)
                     /locus_tag="IWI22_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9168696.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(23476..23946)
                     /locus_tag="IWI22_001296"
     CDS             complement(23476..23946)
                     /locus_tag="IWI22_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9168697.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(23994..24908)
                     /locus_tag="IWI22_001297"
     CDS             complement(23994..24908)
                     /locus_tag="IWI22_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9168698.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            25054..25857
                     /locus_tag="IWI22_001298"
     CDS             25054..25857
                     /locus_tag="IWI22_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9168699.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            26035..26982
                     /locus_tag="IWI22_001299"
     CDS             26035..26982
                     /locus_tag="IWI22_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9168700.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(27050..27343)
                     /locus_tag="IWI22_001300"
     CDS             complement(27050..27343)
                     /locus_tag="IWI22_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9168701.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(27371..28036)
                     /locus_tag="IWI22_001301"
     CDS             complement(27371..28036)
                     /locus_tag="IWI22_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9168702.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(28176..28808)
                     /locus_tag="IWI22_001302"
     CDS             complement(28176..28808)
                     /locus_tag="IWI22_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9168703.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(28815..29882)
                     /locus_tag="IWI22_001303"
     CDS             complement(28815..29882)
                     /locus_tag="IWI22_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9168704.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(29879..30610)
                     /locus_tag="IWI22_001304"
     CDS             complement(29879..30610)
                     /locus_tag="IWI22_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9168705.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(30778..31257)
                     /gene="greA"
                     /locus_tag="IWI22_001305"
     CDS             complement(30778..31257)
                     /gene="greA"
                     /locus_tag="IWI22_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9168706.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(31393..32568)
                     /gene="mltG"
                     /locus_tag="IWI22_001306"
     CDS             complement(31393..32568)
                     /gene="mltG"
                     /locus_tag="IWI22_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9168707.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            32733..33893
                     /locus_tag="IWI22_001307"
     CDS             32733..33893
                     /locus_tag="IWI22_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9168708.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT        10146 a         6929 c         5620 g        11322 t
ORIGIN      
        1 aaaaaagaag ctgaaaagca actaaattag ttgcttctcg gcttctttta ggcatcttca
       61 acttcaaatg tcaattattc taaaatttat tggattaagt catagatttc catcgctacg
      121 atatcgatat tatcaaattc gtattgctga tgagtatctg tttcagttaa ttcaaatgta
      181 tcggtcgctt tatcataagt tactgtacac ttctctacgc cttctttttc aaaacggcga
      241 acttgcagtt cattatctgc tgactcgacc atagcctcca gtcgttttat gattgcgact
      301 aattgtgaat gtgtcatcgg aggtcctcct tttcttattt ctgttatatt gtatcaaaag
      361 catagcctac ttgcattgat tttctctaaa aaaactcgtt ttttatgatt ttttttctgg
      421 tatttttcct tcaggccacc aaagtttgat caaagcttgg gttccgtcat tctcaaaacc
      481 tttatctgcc aactcttcat atagtttcaa tgcttgggta gttgctggca atacaaggtc
      541 cattttttct gcttcttcta atgcaatctt taaatctttg ataaaatgtt tgacaaaaaa
      601 accaggtgtg taatcttctt ttaaaatacg aggagaataa ttgcttaaag accagttagc
      661 tgcacttcca cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt ttttttgtgc
      721 atagacaagc atttccgtca ttcctgtcat cgtacctgca atcatgatct gattagccat
      781 ttttgtatgc tgacctttcc cagcagaacc atgcaatgta aatgtcgtac caaattcttt
      841 gaataaaggc agtacttgat cataaacggc ttcttctcca ccgaccatga tcgtcagtgt
      901 accattcttt gctcctaagt caccaccgga taccggtgca tctaacgcat ctgcgccttt
      961 ttcttttgct gtttttgcaa ttttttctgc caggcttggt gtacttgtag tcaagtcaac
     1021 taaaatatgt cctgagatat ctgcagaaaa aatccccgac tgtccatagt agatttcttc
     1081 tacatctttg gggtaaccta ccattgtaaa gagaatatta ctttgttctg ctaccgcttt
     1141 tggtgtatct gcccagatag ctccttctgt tactaaagga tcagcttttg cttttgtccg
     1201 attatagaca gctacttcgt gtcctgcttc tagtagatgg cgtgcgacag cacttcccat
     1261 gacgcctgtt ccgataaaac caagtttcat tttttcgacc tcctaaaaat caaaaatcct
     1321 tgcttctatg ctagtatacg ctagaaacaa ggattttttc ataaaaaatg tttgaatttt
     1381 atcttttaaa tgaatttaat tttattttga ttcgttcaat cgagtcttct tcttcttttc
     1441 ttcgcatctc ttcttcatga taaacaacca tgtcttgata aaattcgatc atagcttcgc
     1501 cgaaatgtgt agatgaaata tcataaagtt tagcttctaa aacttttgga tctttaggaa
     1561 cttcactgga aatataatca ataagtgctg gtgcaaagtc ttcgtcggtt tcaaaagttt
     1621 tccctaatga aggatgatcg aacaatcggt tcaaataagc atttccttcc acgacacaag
     1681 gtgttccggc tgccatcgct tctgtataag taagcccttg tgtttctgaa gtagaagcac
     1741 tgacaaaata atctgccgct ttataataaa gtgctacatc attgttgtcc acttctcctg
     1801 tgaactgcac aaattcttct aactgcagtt ctgagatcaa ttcctctaac tcttctacat
     1861 aaggtccttt tcctacgata actaatctgg cgttaggata agcggagatg acctgaggaa
     1921 acccattaat caaggcttgg atattttttt cataagaaat cctgcttaaa gaaagaatca
     1981 tcaaatggtt ttcctgaaga cctagtgact cccttaattt gctgatatcc tgctgagtga
     2041 tatcagaacg tttgaatttt tcaatgtcga taccagtagg aatcacgcga agcggtgcta
     2101 ttacaccata gctacgtaat gtatcaataa cacgttcact tggacaaact acacctgtcg
     2161 tatgatttgc aaataatcta gacaagtatt ttacatggga ttgcctgatc acttttcctt
     2221 taccgatata atgaaggtag tcttcataca tcgtatggta agtatggatc agaggtattt
     2281 tcaacttctt agctaccatc ttcccaagta tccctgctcc gaattctgta tgggtatgaa
     2341 taagatctag ttctagttct ttggcaacca aataagcata ccacatgcct ctaacaacaa
     2401 ctcttcgatc cttgaaagaa ataaatggta cacttggcat tcgaatcaca tctttttcca
     2461 acttatccgc attaggatcg gtcgttgtaa aaatatagac ttcatgccca tgtctttcta
     2521 attcttgttt taatgttttg atcgatgtag caactccgct gacttgcgga aagtaggtat
     2581 cggtaaaaaa gccgattttc atgtttttcg cctccaaact agtccttgac ctgatttact
     2641 ttttttaatt tgctagagac tttttcattc agtacacttt cgtatacatt tttaagttct
     2701 tcaccgattt tattgatact tcgctctttt gctgtttgat aaccggcttg tgtcgtactc
     2761 ggtagttctt gattggtaat ctgttggatc agtcggataa attcttcgtt tgtcttcccc
     2821 atatagcagt tcttgctatc ttctaaccag ccattataca ctggaatatc tcgaacaagt
     2881 acgttttgtt tgcttgctaa agcttctaaa acaacgatcc cttctgtctc ttcataagaa
     2941 gggaaaaaga atagatctgc acctgaatac gcaccttcta tgatctctcc acgaatgtaa
     3001 ccaggaaaca ggacattttc aggatgatct tcttttacaa ttttccggat gttccgagga
     3061 atcgagtaca tgggaacatg accaaaccaa ataaatgtgt attcgggcat cttcttagca
     3121 atttctacga aatcaatgat tcctttacgt tcgaaaaaca aaccgacaca aatgattact
     3181 ttttgatccg gagacagttt gaaatattct ctgaactttt gttctttcat tggatcaggg
     3241 cgatatttct ctaagtcgat cccattggaa atagcttgaa tcggcacacg gatactatag
     3301 ctttccaaca actgttttga ataaggtgtt ggcgtgatca gatagtccgc tttttggtac
     3361 aatcctacca gatatttttt tacgatagga gaaagctggt tcgatccgat aaaggaattt
     3421 cggaaatctt cctctgtaga atgcgcatga tagacaactt tttttccttc tcttctggct
     3481 ttattgacca tattatgact attgattccg tatgtattga tgtgcaggat gtcataatct
     3541 tctttttcat ctagtgtata agagataccg acttccgtca gagcacgttt ttgatgatca
     3601 agcgcacgcc cgattccaga ttttgcaagc atcttttcac tttcaaaata taataatacc
     3661 ttcaccttac gtcccccttc acttatatag tataacatag cctacaaact gtcactcaaa
     3721 cttaagccct atttttatct attcttactg acaaaaacgt tttcttaaaa aagaacaaaa
     3781 aaactggcgt agctctttta caagccacac cagcttttga tacgttgttt attttacata
     3841 ttcatgaaca agttcaacga cttcttccat cgtatcgcat tctttcaatg cacgatcagc
     3901 aagttcagcc attttcgctg tgtccaagcg tttcattaaa ctacgcgttt taagaataga
     3961 tgtcgcactc attgagaact catctagacc catacctact agcaatggaa cagctgtttg
     4021 atctccagcc atttctccac acatacctgc ccattttcct tcagcgtgtg ctgcatcaat
     4081 tacatttttg atcaaacgta aaattgatgg gttatatggt tggtacaagt aagaaacgcg
     4141 ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa tcgttcgtac ctacagagaa
     4201 gaaatctact tcttttgcaa acttgtctgc taagactgct gctgctggaa tctcgatcat
     4261 gatgccgact tggatcgtat cagatacttc cttgccttca ctgatcaatt tttgtttttc
     4321 ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt gtagctacca tagggaacat
     4381 gatacgtaag ttaccatgaa cagatgcacg aagcaatgca cgcatttgtg tacggaacat
     4441 ttcgtcgcct tgttcagata aactgatacg caatgcacga tatcctaaga atggattcat
     4501 ttcatgaggt aattgcagat atggtagttc tttatctcca ccgatgtcca ttgtacgaac
     4561 aactacaggt tttccttcca taccttctaa tacagctttg taagcttcat attggtcatc
     4621 ttctgttggg aagtctggag aatccatata caggaattca gtacggtaca gaccaactgc
     4681 ttctccacca ttattatgga caccaactag atcttttggt gttccgatgt ttgcagctaa
     4741 ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt tttaattttt cccattctgc
     4801 tttttgtgct gcgtaatctg cacctgcttt ttcaaattca gctttttgtt catctgtagg
     4861 atcgatgatt acatcgcctt cgatcccgtt gacagccaaa atatcgcctt ctttgacttt
     4921 agcagtaatt tcttttgtac caacgattgc tggaatttct aaagaacgtg ccatgatagc
     4981 agaatgtgaa gtacgtccac caatgtctgt tacaaacgct tttacaaaat tgcgatctaa
     5041 ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg accacttctt cgttgatcat
     5101 tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa atacgtttag caacatcacg
     5161 aatatctgca gcacgctctt gcatataagc attatcttcc attgcttcga acataccgat
     5221 atacatatcc gttacttctt ttagcgctga ttcagcattt acgctgttgt ctttaatatt
     5281 ttgcttgatt tgacctacca tttctggatc tgaaagaacc atcaaatggg catcaaatac
     5341 ttgcgcttct gcttcaccta agctttgcgc tgctttttca cgaatttgct gcaattcttc
     5401 agtagattta gctaatgcag catccaaacg agcttcttca gccgcagtat cttctactgt
     5461 tgccttactg aatgttaaat ccggttgaac tagcaggtaa gcttttgcaa cggctacacc
     5521 gtcactagcg gcaatccctt ttagcatttc aaccattatt cagataagcc ttctttcttc
     5581 attgtttcaa cgattgctgc cattgcatca gcttcgtcag caccttctgc agtaatagta
     5641 acatcagaac cttgacctac acctaaagac ataacgccca tgattgattt caagtttact
     5701 gatttacctt tatactctaa gtttacatca gagttaaatt tgcttgctgt ttgtactaat
     5761 agtgtagctg gacgagcgtg gatcccagtt tctgctacta cgtgaaattc tttcttttcc
     5821 atattaatcg gtctccttta agtgagtgtt gtttttatgt gagggttttc ttttttagta
     5881 attcagagaa tcctaacaaa tgccaccctt tcatctgtaa agattaacat tttttttcgt
     5941 tagatacaac agttttcatt caatttcaga taaaagtatt caaattattc cggattcttt
     6001 ttcatccttt tcttcacatt ttataacaaa cgtgtttatt ttctttttct tgtcgctttc
     6061 tcttctcctg acgtttatca ttctttcctt tgaccctctt tgttttcata tgaaacgaat
     6121 tacttgcttc cctttttatt tttagtataa taagagtcaa aggtcaggaa tggtcaatag
     6181 agatttcacc ttttctacct cataaatctc ttaaaaaact aggggttatt cttttacctc
     6241 atgaaaaacg agatgtaaaa tatggatgta cccatttttg tgaaaggacg tgaatgattc
     6301 atgctttgtc aaaactgcgg caagaatgag gcaacgattc acttgtatgc cactgtcaat
     6361 ggtcagcgga cacaactcga ttactgtcag agctgttatc aaaaaatcaa aaatcaacaa
     6421 aatggaggtc tgactaatat ggcccaaaat gatccatttg gatttggcag tttggacgat
     6481 cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca aacaccacca
     6541 acacaatttg gcggaaacaa caacttcaat ggtggtcagc cacctcaagg caatacagaa
     6601 ggtttattgg gagaatacgg aatcaatatc acagaacaag cacgacaagg tgatatcgat
     6661 cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa tcgccgaacc
     6721 aagaacaatc ctgtcttgat tggagaacca ggtgttggta aaacagcagt tgttgaaggt
     6781 ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa agaagtcatt
     6841 cgtttagatg tcgtatcttt agtccaaggt actggtatac gaggacaatt tgaagaacgt
     6901 atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt catagatgaa
     6961 gttcacgaaa tcgtcggtgc tggttctgct ggtgatggaa atatggatgc tggtaatatt
     7021 ttaaaaccag ctttagctcg aggagaattg caaatggttg gtgctactac cttaaatgag
     7081 taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt acgggtagat
     7141 gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg ttatgaagac
     7201 tatcatcatg taaaatatac tgatgaggcc atcgaagcag ccgcaacttt atccaatcgt
     7261 tacgtccaag atcgcttctt gccggataaa gccatcgatt tgctagatga aacaggttct
     7321 aagaaaaact tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa actgcaagaa
     7381 gcagaagaac aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc agcttattat
     7441 cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc agctcactga agaggaaaca
     7501 cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg tattccagtg
     7561 ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact tagcagatga cttgaaagct
     7621 catgtcatcg gacaagataa cgctgtagat cgagtagcga aagcgattcg ccgaaaccgt
     7681 gttggattaa acaaacagaa tcgtccaatc ggctccttct tatttgttgg accaactggt
     7741 gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac tattcggttc acaagattca
     7801 atgattcgtt tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa attgattggt
     7861 tctcctccag gttatgtcgg atacgaagaa gctggtcaat taacagaaaa agtacggaga
     7921 aatccttaca gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga tgttttgcat
     7981 atgttcttgc aaatcttaga tgatggtcga ttgacagatg cacaaggaag aacggtcagc
     8041 tttaaagata cgatcatcat tatgacaagt aatgccggaa ctggtaaagt agaagcaaac
     8101 gttggttttg gtgcggcacg cgaaggtgtt actcgttctg tattgaacca attaaacaac
     8161 tactttacac cagaatttct gaaccgtttt gacgggatca tcgaattcag tgcattatca
     8221 aaagaaaact tgatgacaat cgtcacacta atgctagacg atgtcaacca aatgctggca
     8281 gcacagcaac ttcacatcga agttcctacg aatgtcaaag aaaaattagt tgacttaggc
     8341 tatgatccat ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca aatcgaagat
     8401 ggtatcgctg aattttatct tgatcaccct tctatccatg agttgaaagc aaaactggat
     8461 aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga atcagccgaa
     8521 gaaacggctg aataaatcca gttttatagc gtgagacggt atttctttga gaaatgccgt
     8581 ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga aattataata
     8641 agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt ggtaaatgta
     8701 acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc ccatctagtc
     8761 aaggtctaca cagccggaaa taccactatt gtctacactg aagcccctga acataatgaa
     8821 attctgctga tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga tgctaaaaat
     8881 tatttcttag ataaaatcaa agacgccgtt taccacgagg atgaaatcaa agtaatcgaa
     8941 ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat taggcgctac attagccgtg
     9001 taaacaaatt ttgaacataa taattaaaga gcgtgggaca aaagtaaaga acacttttgt
     9061 ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagcta
     9121 aaattcacga aaattgcttt tcaaattttt gtgaattcca gcttatttct tggagttaaa
     9181 cgcttctgtc tcagcctctt tttctttcta gctttttgat ttttctcttt tcttttgtac
     9241 ttttctcttt tcattttcag ttctacgcgt gtctttattt ttaacttttt cctctttatg
     9301 gatgattgat cgtacttttt gtgcaatttg ctcgtttttg tcccagtctt ttaaatctaa
     9361 taatcgacat aatcgtaaca tctcttcttt tttctgtttt tttctttcac gtatttgcca
     9421 ttctgtttcc atgtgtcaca ctctcctttc tttaatcata tgtacgcaaa ataattttta
     9481 tatttttttg attagacatt tctatgattt taatatagta aaaagagccg tcacggaaaa
     9541 tccgtcacgg ctctaaacaa aatatgagag actgtttttg tttggctatt cttattactt
     9601 gatgcagggc agcttgtgtc aagacttctt tacatcaaac tttttaattt tacattagga
     9661 tatttatccg caaaccagcg ttctgcaaat tggttctcga ataagaacaa tggttggtcg
     9721 aaacggtcac gagccaagat attgcgactt gaactcatct tctcatccaa atcttctgga
     9781 tcgatccagc gagcgatttt gtgtcccatt ggagacatga ttacttcggc gttatactca
     9841 tttaacatac gatattggaa tacttcgaat tgtagctgac caacagctcc aatgatatat
     9901 tcttccgtta gatatgtttt ataaagctga atcgctcctt cttgaactaa ttgatagatt
     9961 cctttgtgga aggatttttg tttcatgaca ttttttgcag tcactttcat aaacagctct
    10021 ggagtaaatg aagggagttc ttcataagca acttttagtt ttccttcgaa caatgtatcc
    10081 ccaatctgat aattgcctgt atcatataca ccgatgatat cgcctgctac tgctttttcg
    10141 acgttttctc tcgaatctgc cataaattgt gtgacattgc ttaatttcag ttttttattt
    10201 gtacgttcca accagacatc cattcctcgt tcgaatgttc cagaacagat tcggacaaaa
    10261 gcgattcggt cacgatgggc tggattcata tttgcttgga tcttgaatac gaaacctgaa
    10321 aattcttcct cataaggact taccatctga tcatcttgtg ttttgtgtcc ataaggagct
    10381 ggtgccaatt caacgaaagt ctctaaaaaa gtctgtacac cgaagttagt caatgcagaa
    10441 ccaaaaaata ctggtgtctg atcgccacga gcaatttttt cttgattaaa tgcatctcca
    10501 gcctctttga ttagttctac ttcttctaag acttgttcat ataaggaatt ttcagttaga
    10561 ggatgatttt cgggaagcag tccatcttca tccaattcta ctaatcgttc atcctgataa
    10621 ttttccggac gataaaactc tacacgatta tggtagatat cataaagacc ttctagccct
    10681 ttacccatac cgattggcca attcatcgga taagattcga tatctaatag ctcttctaat
    10741 tcttctaaca attccaatgg ttcacgacca tcacgatcta gtttgttgat aaaagtaaat
    10801 atcgggatgc cacgtttttt tacaacttgg aataatttct ttgtctgtgc ttcgatccct
    10861 tttgcactat cgattaccat gaccgcacta tccacggcca tcaaagtacg ataagtatct
    10921 tcagaaaaat cttcatgtcc tggtgtatcc aagatattta ctcgtttccc ttgataatca
    10981 aactgcataa cagaactagt aacagaaatc ccacgttgtt tctcgatttc catccagtct
    11041 gatttggcaa aattacctgt ttttttgcct tttacggtcc cggcttgacg gatagcccca
    11101 ccaaacaata gtaattgttc tgtaatcgtt gttttcccag catccggatg ggaaatgatc
    11161 gcaaaagttc tgcgattgtc cacttgttgc tttaattctg gattattcat aacctaactc
    11221 catttcttcc attcaattat ttatattttc tctaggcaca gattgtccga tctcttaaag
    11281 aaagacaatt tcatataggg ctgtgacaaa agccgtgtag cagtctcttt ttccgagtaa
    11341 acggcgttct agagacagcc tatcagcaat aagcccaccc aagccaaaaa tacgaaaagc
    11401 tgtttttgct tggctgctct tattacttaa tgcaggatag ctttttcaca acctttgtca
    11461 actactacaa ctgaatcagt atagcatgga ttcatcgaat gaaaaagcca ccaaaccata
    11521 agagtcaaat atttttttgt cagctgacta aaagaaaaga cgtgttgctt gtttattcaa
    11581 gcaacacgtc tttcataacc aagtttccaa ttatcttctt ggctcgtcat cttcgaattc
    11641 ttttctgaag tatcttcgtt cttcttcagg ttccgcttct gtttcttccg gatcatgaaa
    11701 aatgacacgg attttaagga ttcgagaacc ttccatttct tcagaaacca gcgtaatatt
    11761 atcgacatca aacgataact tttcgccttc atccggaatc atgcctaatg ctgtaatcaa
    11821 ataaccagcc atcgtatcta catcactcat atgcagatca ctgtcaaaag cttcattgaa
    11881 ctcatcaatc agcatacgtc cttggatgat atattcgtgt tcatcgattt tttcataaag
    11941 attctcaact tcatctgatt catcatcgat ttctccgacg atttcttcta aaaggtcttc
    12001 tagtgttgct aacccaacga cgccgccata ttcatctaat aaaatagcca tctgattctg
    12061 cgtctttttc atttcatata acagatcatc aataaagacc gtttcaggaa cgaataaagg
    12121 ctcctgcatg attttcttga tatctaaatt atcaaaaccg aatttatggg cagcttttag
    12181 taaattcttt gtatgaagga ttccgactac tttatctttg tcttcgttat aaacagggat
    12241 tcttgaatag ttttcggata aaacttcatt cacattttct tccacagtgt cgttgatatc
    12301 aatcataaat gcatccgttc gtgggaccat gacttctcgg gcaactttcg tatcaagaga
    12361 aaaaacccct tggagcattt ctaattcttc attattcaat acgccttcgt tttcaagcat
    12421 ataacgcatt tcatctctgg tcatttttga atcttcatca tcaaaagtca tcggcgtgat
    12481 acgcgccaat aaacttgtgg aagcagaaag cagccagacg aagggcttag ctactacacc
    12541 aatcaagcgt atcagccctg aagtgaactt tgcgacttct tctgatttat ttaaagcaat
    12601 acgtttaggg tacaattctc caaaaacaat cgatacatag gtcaaaatag ccagcacaat
    12661 gatatttgca atattttttg ccgctgtgcc gccacctaat accggagcta accgttccga
    12721 taaagtatta gctagtgaag caccagacaa aatgttgacc aaagtgattc ctacttggat
    12781 tgtcgatagg aaattatttg gattctgcaa tatttttaaa agcttttgag atttcacatc
    12841 tccttcatca gctttttgct cgattcgatt cttattgatc gaaacaacag caatttcgga
    12901 agctgctaaa aaagcattga tcaatgtcaa aacgatcaat aataaaatct gcgctaacag
    12961 cgactgactc tcagggtcag cattcataat cgcattctct ccttataaaa ttaattttta
    13021 gtttacgata gaaaaaaacg tggcactata gccacgttaa aatggtatca caaagcaatt
    13081 ttctttgcaa taaaaacaag tgtactaaaa ctacctacga ggtcgtagca attttcttct
    13141 tgttttcatt ttgcttgtct tctttgacga ttttgattat ataggaaatg atcgtcttgc
    13201 agattgcgta aaaaggaaca cctaagaaga taccaagcaa tccggcaata tttccagcta
    13261 ctagaagaac gataatgatc gttaatggat ggattttcag tgatttccca attacattag
    13321 gataaatgat attcccatcc aactgttgga cgattagcac aaccacacaa caaagtaatg
    13381 cttttaccgg atcattgaag actgttacta gaaatgcagg tgctagtcct agataaggtc
    13441 ctaaatatgg aattaggttt gtcaatcccg caatgacccc aaataagaaa gcataatcca
    13501 caccaattac catatatcct aaaaatgtga aggtaccaac gaataaacat tcgattgctt
    13561 gtccgctaat gtaattagcc aatgttttat tcagttgccc caataactcc acaatttggt
    13621 ttcttctttt ttctgggaaa aactgttgga tgtttggcac tagtttttct ccgtctttca
    13681 acatatagaa caagatgaat ggtgctgtaa tgattactac ggttgccgaa gcgatcgttc
    13741 caacgatcga acctaaacta ttggatactc cactaaggaa ttgctgaatg attgtcccgt
    13801 aagaaatgtc taattgctca aaatatttat tgatatctac ttccttgaac aaaggcaatt
    13861 cggctacctc ttttaaccag ctttctacct gtttgatgaa atcaggcata ttagatgcca
    13921 atgaggaaat ttgctgtact agacttggga tgacactaag taaaatccaa ataattgctg
    13981 caactaaaag gagcagcaca attgcgacag cccatattct tttgattttt gctttcatca
    14041 acagcttgac aataggattc aataaatagt aaagaaagcc tgcaatcagt acaggtgcaa
    14101 ataacgtgga aaaaaatgtc ccgatcggtt cgaaaataaa gtttatttct gatgaaatca
    14161 tgatcaatgc agctatgatc aataattcaa gcgaccaaaa cattagttta gactgtctaa
    14221 gtttttcaaa caatagattt cctcctttct ttcttactag tatatattat attcgttaaa
    14281 aattctttta ttcttttttt atttttgaaa acttgccata ctttttattt gttttatttt
    14341 ccagtatact atacgaaagg aaggtggcta gaccatgaaa gttgtacaaa caaaagatac
    14401 aatgagcaat atttacttag atgctgtacg gatcaggcat caagtttttg tagtcgaaca
    14461 gggtatgcct ttgtctcgag aaatagacaa agatgaagcc cattgtatcc acttcgtcct
    14521 gtactcagat aaaaaagaac cgcaaggaac tgtacgtctt ttgcctttag aaaacggaaa
    14581 aatgaaatta cagcggatgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
    14641 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
    14701 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
    14761 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
    14821 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcggata attcatcatt
    14881 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
    14941 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
    15001 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
    15061 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
    15121 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
    15181 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
    15241 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
    15301 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
    15361 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
    15421 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
    15481 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
    15541 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
    15601 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
    15661 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
    15721 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
    15781 gctccagaat gattttgaag agccgcactg atataataaa cgtcttttgg ctttgctttt
    15841 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
    15901 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
    15961 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
    16021 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
    16081 actcgcatca cgttgccgtc aaccgcgggt tctggaagtc caaatgcgat acttgcaatc
    16141 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
    16201 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
    16261 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
    16321 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
    16381 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
    16441 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
    16501 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
    16561 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
    16621 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
    16681 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcca tcgcttgttg
    16741 gatcacttct tgagaaaaac ctttcgtcat caatgtttgt tggatttttc tcaataattc
    16801 tttttggctt tttccgcgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
    16861 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
    16921 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
    16981 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
    17041 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
    17101 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
    17161 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
    17221 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
    17281 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
    17341 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
    17401 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
    17461 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
    17521 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
    17581 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
    17641 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
    17701 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
    17761 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
    17821 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
    17881 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
    17941 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
    18001 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
    18061 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
    18121 aaaaaacgca atgatctgtg agatcaatga acgactgccc gaagttgttt caatcatgca
    18181 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
    18241 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
    18301 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
    18361 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
    18421 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
    18481 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
    18541 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
    18601 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
    18661 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
    18721 taaaaagttc aagattgaat atttacagtc tgtcgacatg tttcctcaga ctgcacgttg
    18781 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
    18841 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
    18901 ccgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
    18961 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
    19021 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
    19081 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    19141 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    19201 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    19261 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    19321 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    19381 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    19441 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    19501 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    19561 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    19621 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    19681 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga accaaatcat
    19741 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    19801 tcatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    19861 aaactactat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    19921 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    19981 ggagaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    20041 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    20101 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    20161 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    20221 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctaca tcatcgcctg
    20281 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    20341 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    20401 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    20461 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    20521 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    20581 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    20641 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    20701 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    20761 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    20821 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    20881 aactggtctt gtttgattgt ttcaaacgca ggggtgtagt tcgtcgctac atcaagcatg
    20941 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    21001 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    21061 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    21121 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    21181 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    21241 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    21301 gccatttcga caccaccaaa tgcataggca tgatttaatt tatctaccgt tccataacct
    21361 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    21421 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    21481 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    21541 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    21601 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac aaatacccct
    21661 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    21721 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    21781 ttaaaaaaca tttttgtgta acgattacta ttttttaaaa tttctcaaaa ttcttaacta
    21841 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    21901 ctttttttac gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    21961 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    22021 ttatcattcg atttcacgaa aatttcttcc tatgactggt ccttttgtta aacccatctt
    22081 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgatttc
    22141 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt
    22201 aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt
    22261 tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc
    22321 ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat
    22381 ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca
    22441 acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc
    22501 gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag
    22561 aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg
    22621 ttgaaccatt taaccgttcc gtgttccata aatgtatagc ctccaaaaaa gttcttgcaa
    22681 gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta
    22741 ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa
    22801 acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg
    22861 gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt
    22921 ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt
    22981 ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc
    23041 ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt
    23101 aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc
    23161 gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa
    23221 taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg catttaaatt
    23281 cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata
    23341 aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt
    23401 cctcctcaat gttttcttta taatgtctac attacaaaga gtaacaaaga gaaggaaatt
    23461 cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc
    23521 agtttcttta aaaataaaca acttgctgaa aatatagttc gctaggataa caataatttg
    23581 agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc
    23641 ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag
    23701 tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc
    23761 aaacaataca gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata
    23821 ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag
    23881 atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg
    23941 cttcatgagc caacctacct tattaaaaat tcttatttat catactctat atttcaataa
    24001 ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg
    24061 atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc
    24121 ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc
    24181 ttttcgctat actgccaaat atggacatga tgaacattct ttaccccatc gattgcttca
    24241 atttcttgtt ccattttttc atagtcaata gcaggtgcac tttgtatcaa aatagcggca
    24301 gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata
    24361 actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt
    24421 accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt caaactttca
    24481 tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct
    24541 actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca
    24601 acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt
    24661 tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt
    24721 gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca
    24781 agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc
    24841 aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct
    24901 tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc
    24961 accaatcatt cagatgtttc cctaaagcac ttgtatttca cttgatactt cgctagaatt
    25021 gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt
    25081 tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt
    25141 gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa
    25201 gtcttaaata acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca
    25261 atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc
    25321 attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg
    25381 atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat
    25441 acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa
    25501 tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca
    25561 ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg
    25621 atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa
    25681 gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt
    25741 gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca
    25801 ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa
    25861 ataaagacaa ctcgttattt ctacttaaat agaccctttt agaggttgtg acattttttt
    25921 gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc
    25981 aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca
    26041 gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact
    26101 atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag
    26161 atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa
    26221 tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa
    26281 catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta
    26341 ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg
    26401 aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt
    26461 ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc
    26521 tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat
    26581 caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag
    26641 gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc
    26701 atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac
    26761 cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat
    26821 cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat
    26881 tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt tgccacccat
    26941 gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt
    27001 tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt
    27061 taaagtgata ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt
    27121 taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatccatt tccactttct
    27181 ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg
    27241 ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg
    27301 agtcaaacca tttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc
    27361 tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa
    27421 taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg
    27481 tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt
    27541 cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt
    27601 tatctgacag ctctaagtag tctagaatat tctttgatac taagttatgt gcaatccgta
    27661 tccccatgtc tcgctcaggg tgtacgacac gatctgctcc gatcttctct agtacacggg
    27721 catggtactc attttgggct tttgctaaaa tattcggtac gcccatttcc tttaccatca
    27781 gagtaactaa gatgctggct tggatatctt ctccaatagc gacaattaca tgatcgaaat
    27841 ttcttatacc taatgaacga agtgtcatct catcctgtgc atttgccaca acagcatggg
    27901 tggctatatt catatattca ttgactcgat cttcattact gtcaatagcc agtacttctt
    27961 gtccagcttc gatcagtgta cggcagatgc ttccgccaaa tcgtccaagg cctacaattg
    28021 caaaattttg tttcaccatt ttcctccttt agtatcttct tttctcagta taacaaagcc
    28081 tttccaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt
    28141 ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttaaac
    28201 gcatagatag ctgcttgagt tcggtcttct acctctaact tagctaatat attcgacaca
    28261 tgcgttttga ctgttttcag tgtgataaac aattcatccg cgatttcctg attacttttc
    28321 ccttcagaaa tcagcatcaa aatttcattt tctcgattcg ttaattcttc atgcaatact
    28381 ggatcatttc gccgactcat tttttccatc atttttgttg tcacttcagg ttctagtact
    28441 cgctcgccgc gttgcgtagc ccttatagca tcagctattt catgtgctgt agacgtcttc
    28501 aacaaatatc ctgctgctcc tgcttcaatt gcaggataaa ctttttcatc atcaataaaa
    28561 cttgtgacga taatgatttt ggcttttggc caatctttta agattgcttt tgttgattcg
    28621 atgccatcca tttcttccat gactagatcc attaaaatga catccggtcg aagtgccatc
    28681 gccttttcat agccttgacg tccattttca gcttctccaa tcacttcaat gtcttcttgg
    28741 atagataaat aagaagagac gcctaagcgt accatttcat ggtcatctac taataaaact
    28801 tttatcatct ttatctatgc ctccttcatc aaagggacct tggtctccac gctcgtacct
    28861 tgccctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata
    28921 ttgttcaatc cataacttcc cgttttggtt tcattcatat taaatcctgt cccgtcgtca
    28981 atgatacgta ataaaagatt gttatctatt ttatgcaaat agacttctaa ttcattagcc
    29041 ttagcatgtc ttaatgtgtt tgataacaat tcttgaacga ttcggaacaa gtgatcctca
    29101 atggaactag ttaattttac atcttctacc tcccatttta gcgaaatctg gattttattc
    29161 tgcaattcct tcaataactg ctcaatgcct tgttttaaac ttttttcttc taaattgact
    29221 ggacgcagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgcc
    29281 accattttca actgcttacg gaacatttct ggtgtctcac ttttttctgc ttgctccgtc
    29341 aatgccgaca tcatcatcat cgctgcaaat aattgctgtg aaactgaatc atgaagctca
    29401 cgagctaatc gatgcctttc taattttaaa atctcttctt tggtttgtcc atctacatat
    29461 tgaggacggc tagtcataat ctgtaattca ctggatactt cgatcatttt ttcttttatt
    29521 ttagtaattt ccatatcaat atctttgatg tacagatcat ccgacgcgct tgggatcggc
    29581 tgatgcaaaa gtttggattc ataattgcca gaagaaagtg cacgaagctt ttcttcgatt
    29641 ttcccgtatt ttgccttttg aataagtgat aacaatagaa atacgatcaa tcctacacct
    29701 aatgaaattg ccatcaaatg gaagataagc ggaacgtata ataaacgcgc acgcagcaat
    29761 tctccccacc aatgactttg gttactggca taaaaataag tgaaaagtga aaatagaata
    29821 ataaggaaag ccgcaatccc cgaataaaca gctagcattg ctttggatat tcttcccatc
    29881 atattcgtat cacctcgaca tctccaacca aagtattggt aatgatcttc aatctgcgag
    29941 gattttcatc ataatcactg ctgcagattt tgatctgttc gttttttaag gaaaactgct
    30001 cttcttcaaa caagacattt cctactaatg tcgcatgctc taaacgaata gccactccca
    30061 ggggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt
    30121 ttggcaaaag tgtattgccc aaatcgatga tagtatcacc agaaatgatg gcaatgttga
    30181 tatcatccca ttcatagacg tcgtttccaa tacgttggtt accaaataat tgctgtcttt
    30241 tacgctcatt attatgtgtt tttaattgct ctgtttggac catcatgatc tgtttttttc
    30301 gccaaaaagc attttttgtc aaatctattc ctgaaatctc tacacctttc agcccgataa
    30361 ataaaacggc caatacaacc atcatccata aagcaggact gttgactaag ctaaaaaaaa
    30421 tgaccaagct ccctaagatc aattgaaaat tttgaaattt agtacgagga tatttgcgca
    30481 tagctagata tatattgaaa ataccgaata ttactaaaag caaaagtcca gtattgttta
    30541 cgatttgcca aacagcaaat aacagcagca gtgcttcgac taccacaaaa aaacgccaag
    30601 aactattcat cgctttttct ccctcctgac acattctcat ttcatcatac ctattttttt
    30661 gactgttggc tataggactt tagatgtaag atcgttcagt gaaacctgca atactcaaag
    30721 agaaaagatc tagagtgcgt catcagtaag atgaactctc tagatctttc agtcagacta
    30781 cgcttgttct actttgacga ttttaactaa catatcgcca cctggtgtag caatggttac
    30841 ttcatcattt ttacgtttgc caatcaatgc ttgcgcgata ggagaatcat tggaaatctt
    30901 tccagaaaac gggtctgctt cagcacttcc gacaatcgtg tactcttctt catctccatc
    30961 aggcaactcg atgaacgtaa ctgttttacc gatcgatacc tcatctgcat ctacgccgtc
    31021 attatcgatg atttgtgcaa aacggatcat attttcaatt gttgtgattc ttccttcaac
    31081 aaatgcttgt tcatctttag ctgattcgta ttcggagttt tcggaaagat ctccgaaacc
    31141 gcgagcgatt ttgattcgtt caatgatttc tccgcgttta actgttttta gttcttctaa
    31201 ttctttttct aatttttctt taccttctag agtcattgga taaactttct ctaccatgtg
    31261 taacgtctcc ttttttcgat aaaataccgc aaaaggtaat cttttgagga atatagtcca
    31321 aaaaagatta ccacgctttt cttaaaatgt aaatgttttt ctaaaaatga tcaatgacta
    31381 gcccctgttt ttctaagaat tatttacata tttttgcact agttccatat gttcttcata
    31441 cgttttagcg aaataaacat ttccagtgct cgtatctgct acgaaataat aatagtcatt
    31501 ttctgctggt tctaatactg ccttgattgc ctcttcgctt ggattgtcaa aaggaccagg
    31561 cccatagccg gtattggtat acaaattgta tggtgaatct acagctgtat cttcatacgt
    31621 caccaattct ttatgttctc ctaatgcata taaaacagag atatctgact gaagtggcat
    31681 atttgccttg attcggttaa agaagacttg tgcaatattt ttacgatcat tttcttttac
    31741 gccttctttt tcaactagag aagccaaagt taaaacttct tgtacagtca aatttttctg
    31801 tttgatagct gaataatagt tttgcatgac cgtatttgtt ttgttcacca tctcaatcac
    31861 aagatcctta agcgaagtac tcgtgtagta atcataagta gctgggaata agtagccttc
    31921 caaacggtat tttgtgttct cagctttaga ggcactttcc aacaattctg ggaatttttg
    31981 atacaactcg ttaaagaaag tctcatcatt tactaaatct aaaaattctt tcttgtcttt
    32041 accagttacc ttcgctaccc gctcagcaat ctgatcgatg tcgtagcctt ctggaatggc
    32101 aattttccca tctgcaactt ttgttggctc agaagtgccg ccttcttgca gctgtttgcc
    32161 aatctcgtca agagtcatat taggcgctaa ctgataatag ccagcttgaa agccagttaa
    32221 atttttaaat tttgtataat aattgaaaac aatcccgctt ttaataatat tgtccttctc
    32281 caaaatctca ccaatctgct tgttagaaga gccactcggg atctccactt ggactaattg
    32341 gtcatctgat ttatctaaag gcttcaaccc gctatctaca taacgataca cggtaaatcc
    32401 aagaaatccg ccaataatta ataatgtaag agcaatcact aaaatgatct tcctcacaat
    32461 cttatcttct tttttacgca gatttctccg cttgtcgatt atatctgctg tagctgcctt
    32521 actggcttct tttttatctt tacttgggtg ttgtttgttt cctgacaaaa caactcctcc
    32581 ttactatcat cttcgtaact ctgtttatta tacatgaaat ttctactgcc gtgaattatt
    32641 ttaccaaata ttatgtcaaa attgtaagtg gcaaatactc tttttgagga ttacaatgaa
    32701 aaatagttaa aaatggatag aaaggaatta acatgtcaaa aggaaaacat cgaaaaaaac
    32761 aaaaaatttc tatttctttt cttgtaattt tattattgtc tgtcagcttc ttttcaacag
    32821 gcatcattat aggaggtcaa ttaacaaaaa tacctcattc ccaaacttat aaagcggagc
    32881 caacacaact tacttccaat ttaacgcccg atgagcagag gcttgctagc aaaattgatc
    32941 aattactaca aaaatcagat tatatcggtt cgatttatgt tcgcaaaaat aatcgcgtac
    33001 tattagaaaa aggatatgga tatgcaaatc tgcgcgtaaa agaatcgaat tcaccttcta
    33061 tttactatca aatcggttcg attcaaaaag caatgactgc tttactgata ctaaaacaaa
    33121 tccaagctgg taagttaact tttgacacaa agttagcaga attttatcct cagattcctg
    33181 gaagtcagca aatcacaatc aaaaatatgc tttatatgcg ctctggtctt catcgaactg
    33241 cttctcccga caaacctatg tcggatagtg aaatcattca gttcgccctt catcatttga
    33301 aacttactga ttatgatacg tatcattatg aaccattgaa ttatacattg cttacaggga
    33361 tattgatcaa actaacgaat cagccttatg aaactcttct aaaaaaagaa atcattcgtc
    33421 cactccatct agaacataca gatttttatg acaatgtaaa aaattcgcct caacatgctg
    33481 tatcttacca gatgtctgca actgacgatt attcaaaagc attgatcgag cctgaaaccg
    33541 acatccgaaa tgaattaggt acaggaaaca taagtatgtc tgtttatgac ttgaacaaat
    33601 tctttaccag tgttctttct ggcggtatca taccaaaaga actacttttc tcattgtggc
    33661 aaaataatgg tgataaacat ccttacagcg gcggtgttta cagtggaaac gattatatcc
    33721 ttgctcaggg gaatatcaat cgattccatg cagtagtggc gatgaaaaaa gacactaaag
    33781 atgcaatcgt catggaaagt aatatccaat cggataagaa catcaaattg ccggccactg
    33841 atttaagaaa tcagatttat gaactgatgg aaggtgttca cttatcaaat taaattttga
    33901 cataatatta gttgtcatct ctgtatagtc aatttagtta gtactctatc tgaaaagtag
    33961 aaataaatta acaaatatta tctgtcctta ttatttgtta atttatttct tatttta
//
LOCUS       DACDNO010000026        33951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDNO010000026 DACDNO010000000
VERSION     DACDNO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.026"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(44..1183)
                     /locus_tag="IWI22_001308"
     CDS             complement(44..1183)
                     /locus_tag="IWI22_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9168709.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINAVFDSLYKNDN"
     gene            complement(1299..1934)
                     /locus_tag="IWI22_001309"
     CDS             complement(1299..1934)
                     /locus_tag="IWI22_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9168710.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLILLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTAKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2017..2439)
                     /locus_tag="IWI22_001310"
     CDS             complement(2017..2439)
                     /locus_tag="IWI22_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9168711.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2457..2777)
                     /locus_tag="IWI22_001311"
     CDS             complement(2457..2777)
                     /locus_tag="IWI22_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9168712.1"
                     /translation="MNPYEKIKELTKQNGISVRELEKRLGYSNGYFSKWKSVSPNSEG
                     LAKVSDYFGVSIDYLLGRDKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            3078..3854
                     /locus_tag="IWI22_001312"
     CDS             3078..3854
                     /locus_tag="IWI22_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9168713.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            3869..4072
                     /locus_tag="IWI22_001313"
     CDS             3869..4072
                     /locus_tag="IWI22_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9168714.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            4088..4426
                     /locus_tag="IWI22_001314"
     CDS             4088..4426
                     /locus_tag="IWI22_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9168715.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(4681..5346)
                     /locus_tag="IWI22_001315"
     CDS             complement(4681..5346)
                     /locus_tag="IWI22_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168716.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDPLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            5401..6099
                     /locus_tag="IWI22_001316"
     CDS             5401..6099
                     /locus_tag="IWI22_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9168717.1"
                     /translation="MQELVILKNKEAVTTSIQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNTKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            6277..6618
                     /locus_tag="IWI22_001317"
     CDS             6277..6618
                     /locus_tag="IWI22_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168718.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            6611..7282
                     /locus_tag="IWI22_001318"
     CDS             6611..7282
                     /locus_tag="IWI22_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9168719.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            7288..7974
                     /locus_tag="IWI22_001319"
     CDS             7288..7974
                     /locus_tag="IWI22_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168720.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            7981..8832
                     /locus_tag="IWI22_001320"
     CDS             7981..8832
                     /locus_tag="IWI22_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9168721.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPSKKSKAKPVRHKYGEYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSESKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERAN
                     RQSKAPF"
     gene            8844..9113
                     /locus_tag="IWI22_001321"
     CDS             8844..9113
                     /locus_tag="IWI22_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168722.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            9275..9595
                     /locus_tag="IWI22_001322"
     CDS             9275..9595
                     /locus_tag="IWI22_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168723.1"
                     /translation="MDKKAAWRKLMLLIQDENWQEDEAVVAEVQRLEKIANGRIRKKP
                     DKRKLRKGKIIVVLYEGNILMQGTARELSAETEYTSGTIRTYAWRNHVDKKGHEYKYL
                     EGSK"
     gene            9592..9981
                     /locus_tag="IWI22_001323"
     CDS             9592..9981
                     /locus_tag="IWI22_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168724.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVGIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRV
                     FKHKKMDYEYEVIGNIYENSELLEEQR"
     gene            9978..10571
                     /locus_tag="IWI22_001324"
     CDS             9978..10571
                     /locus_tag="IWI22_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9168725.1"
                     /translation="MNKQETIKKLESIKAIGNDAIAACYNESMNAGITLMKKIDEPQK
                     HVMPKFFDKWAKQVLEKRDKFYAISLIARAGWGYGVDFELSENGLSSENKELLYWLVD
                     KCSDNYPNKKKAIEALLYGYEVEKEPLYHVKLKIPGVTYYLIQTFLMPGDTHMCFSAA
                     TERYGAKWKNTFTESEIKAIDERYWPFAVPVEEEAEG"
     gene            10568..10945
                     /locus_tag="IWI22_001325"
     CDS             10568..10945
                     /locus_tag="IWI22_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168726.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQQIVFEWLKLTAPTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKEQFEVLAAFAQWGLEQEEAE"
     gene            10942..11145
                     /locus_tag="IWI22_001326"
     CDS             10942..11145
                     /locus_tag="IWI22_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP9168727.1"
                     /translation="MNNRHRRITKLRKQELNVLKAKFEKEYGVSAEEAYKVASQCVAD
                     ASNAIRKFGISILSDDRKWEDIE"
     gene            11142..11351
                     /locus_tag="IWI22_001327"
     CDS             11142..11351
                     /locus_tag="IWI22_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168728.1"
                     /translation="MKVYVVRFGNKFYRSDERSIGNNVLFLVDFLQNARWFDNLEEAN
                     QVSRRLGGLTQVYELVTIDHEGVER"
     gene            11348..11641
                     /locus_tag="IWI22_001328"
     CDS             11348..11641
                     /locus_tag="IWI22_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168729.1"
                     /translation="MKLKDGFYASSHGIGGLMLGMPTKNPKTRKKPKFKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            11671..12111
                     /locus_tag="IWI22_001329"
     CDS             11671..12111
                     /locus_tag="IWI22_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9168730.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            12186..12653
                     /locus_tag="IWI22_001330"
     CDS             12186..12653
                     /locus_tag="IWI22_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP9168731.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGMSIYVTAQRNNTSEESVKAESNKIIIQFASSLELVAFK"
     gene            complement(13063..13416)
                     /locus_tag="IWI22_001331"
     CDS             complement(13063..13416)
                     /locus_tag="IWI22_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168732.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
     gene            14347..14691
                     /locus_tag="IWI22_001332"
     CDS             14347..14691
                     /locus_tag="IWI22_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9168733.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            14696..14977
                     /locus_tag="IWI22_001333"
     CDS             14696..14977
                     /locus_tag="IWI22_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168734.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            15080..15394
                     /locus_tag="IWI22_001334"
     CDS             15080..15394
                     /locus_tag="IWI22_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9168735.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            15372..17066
                     /locus_tag="IWI22_001335"
     CDS             15372..17066
                     /locus_tag="IWI22_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9168736.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPGLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENAGTGFTPLVF"
     gene            17086..18264
                     /locus_tag="IWI22_001336"
     CDS             17086..18264
                     /locus_tag="IWI22_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9168737.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRAQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYKLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            18227..18913
                     /locus_tag="IWI22_001337"
     CDS             18227..18913
                     /locus_tag="IWI22_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9168738.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKREEKIAAFTAAL
                     NKTFGQGDVK"
     gene            18913..20073
                     /locus_tag="IWI22_001338"
     CDS             18913..20073
                     /locus_tag="IWI22_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9168739.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTRA"
     gene            20083..20958
                     /locus_tag="IWI22_001339"
     CDS             20083..20958
                     /locus_tag="IWI22_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168740.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            20955..21266
                     /locus_tag="IWI22_001340"
     CDS             20955..21266
                     /locus_tag="IWI22_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168741.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            21256..21609
                     /locus_tag="IWI22_001341"
     CDS             21256..21609
                     /locus_tag="IWI22_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9168742.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            21599..22000
                     /locus_tag="IWI22_001342"
     CDS             21599..22000
                     /locus_tag="IWI22_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168743.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            21993..22397
                     /locus_tag="IWI22_001343"
     CDS             21993..22397
                     /locus_tag="IWI22_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168744.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            22409..23017
                     /locus_tag="IWI22_001344"
     CDS             22409..23017
                     /locus_tag="IWI22_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9168745.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            23037..23399
                     /locus_tag="IWI22_001345"
     CDS             23037..23399
                     /locus_tag="IWI22_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168746.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            23402..23584
                     /locus_tag="IWI22_001346"
     CDS             23402..23584
                     /locus_tag="IWI22_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168747.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            23601..27020
                     /locus_tag="IWI22_001347"
     CDS             23601..27020
                     /locus_tag="IWI22_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9168748.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFIDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAVATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            27071..27808
                     /locus_tag="IWI22_001348"
     CDS             27071..27808
                     /locus_tag="IWI22_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9168749.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFTCTGLSKIQIYRNGLR"
     gene            27818..30109
                     /locus_tag="IWI22_001349"
     CDS             27818..30109
                     /locus_tag="IWI22_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168750.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNVGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            30133..32376
                     /locus_tag="IWI22_001350"
     CDS             30133..32376
                     /locus_tag="IWI22_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168751.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGNYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAETEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGV
                     DYSPADGKKILIRYRFVDNGTGKIVLDLPINSNSITQEWKEFSITGTTPTFGPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGPTATPFQPNLLAEPYNMCREYPNENIVNPTVKF
                     PIKTSNNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQLQHQATLLP
                     VEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEKGNTRTPNIS
                     EYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQ
                     LTASINEVDTTAKDARTKANANATAIDELDNKIDERINDAATTAFYGAQGEVSNKTKV
                     AEYGVGTGYTTTAVGETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLY
                     KDGTSVDNLVSYGVDALQNRFAASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTL
                     VLTRLSD"
     gene            32454..32696
                     /locus_tag="IWI22_001351"
     CDS             32454..32696
                     /locus_tag="IWI22_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9168752.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQVYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            32712..32909
                     /locus_tag="IWI22_001352"
     CDS             32712..32909
                     /locus_tag="IWI22_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9168753.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMIVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            32920..33939
                     /locus_tag="IWI22_001353"
     CDS             32920..33939
                     /locus_tag="IWI22_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9168754.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT        12024 a         5166 c         6884 g         9877 t
ORIGIN      
        1 gatacagggg tttgttttgt gtttgtgcca aaaatgtgcc aaactagtta tcatttttat
       61 ataaactatc gaacacagca ttgatttttt ctttatcctg atcttcaagc tctcgaacaa
      121 tatgaagata agtagacatt gtagtttcta aactgctatg tcctaaacgt ttcgatacgc
      181 taagtatatt tactccttga taaagtaaaa tagatgcatg tgtatgcctt agtccatgca
      241 aagtaaattg cttttcaatc cctaattctt ttaatttttt gcgcaaatat ttgctgacgg
      301 cgtttgatga aactaatcca ttttttaagt tgaaaaaaac aaaattatga gggtttttaa
      361 cttcaaaatt ctcgaataat tctttttgat tgattttaaa cttttttaag agatcaatcg
      421 tatgtttatc aatggaaatt ttcctatttg aagtttcgtt ttttgttttt ccccattcat
      481 ttaatttata attccatgtt ttattaatag tgatgatttg atcttcgaaa tcgatatctt
      541 cccatgttaa tcctagaagt tcagcaaatc gcattccagt agcaccagca actaacacga
      601 gcataggaga agagtactgc gcagaaaggt tttcttctac gagtttcatt aaagatttaa
      661 attgatcata atctaaatat ttatcttctt ttggcttcaa tgaatctttt cctttgatta
      721 ctgcttttct agttggatca aacggaatta atccttcttc tacggcatct ttcaacgatg
      781 ctctaatatg gttattaaac ttaataacgg tggattttac atggtctttc gcatacttat
      841 ttaaaaagcg ttgatatcca actctatcta aatcagaaat caataccgct ggcatatatt
      901 tctttatgtt cattaacgta tcttcgtatt ttctataagt gataggagat actgttcctt
      961 ctttataaag ttgcatccaa tcctcaaagt aatctgataa taatagattt ttgcgatccc
     1021 ctttaaggcc tttgttcaag ttatattcta attcgttggc ggcatctttt gcttcgcctt
     1081 ttgtcttaaa tcctgatttt cttatcttcg tgtattttcc gtcatctttt ttgtatgaaa
     1141 tttcgtattg ccaactattt ccacgtttga ctaatcttgc cataattgaa tcagctctct
     1201 ttctctgata caataggaac tataaagaag cctactgtat aggtttgttt tttcatagaa
     1261 cacgcttgct ttggacggtg ggcgtgtttt tatatcaatt agttatgaag agcaattgaa
     1321 actgtctctg ttccaaagta acctgtttgt gcttgcaata cattattttg tgcatttgct
     1381 tgggcttcag agatatcaaa aactatttta ccagtttgtt gcatatcagg atttaagctt
     1441 tccataaaga aagagttagt gatattacca ttttcatctt gatttgccga catagatgca
     1501 gccgaatcag cttcgaaagt ttttccatct gcttttaatt tgaagaatga gctatcaaca
     1561 gtcacagcct tatcacctgc attttttaca gataagtcaa ctactaagaa agtgtctttt
     1621 gcgttagtag gaagtacaga tgggccaact tgttttacta cttcaacgga gtttactttg
     1681 tattccattt tccctacagc aacgtcatca ccgattttat aagtcttttc ttcttttgct
     1741 gtagtttctt ttgtagtttc tttattagta cttgttgatg ttgcagtagc tgaatctttg
     1801 ttttctccgc cacttagagc accaccaata ccaaaaaaca aaagtaatat taatacccaa
     1861 aaccacactt ttttgtaaaa aggtttacta actttataca ttttcccatc ttgacccata
     1921 actttttttg ccatttaaat attcctcatt tcttgttata atatatttgt gatctcagaa
     1981 atgaggtatg agtccgtgtt gcagcacggg ctttttttac tgtgcataag agtatttttt
     2041 cttgaaataa gactggcaaa cattaaaaca ttctgttctt aacttattat tgatagaata
     2101 gaactccatg aaattttcta atttgaactg agattcatca gtcagttcgt tctcaataaa
     2161 gatatttagt aaaatcataa ttgcgattct atcagcttca gtttcgaatt ttgaatgaaa
     2221 agttgtagag ttatcgtaca gtactgaata ttcaaaatgt gaagcaatga aatgaccgag
     2281 ctcgtgggct aaatgaaaag cttcagaact gtcttcgtgt agtttttcat tcagaaatac
     2341 tattcttggt tttggataat aaaaacctgg ttcttccatt tccatataga ttaactttaa
     2401 attatactca ctcagcattt ctttcaactt taaatacata caacccatca ctccaactat
     2461 tcattttcct ctaaagcttt agcaattgca atcgctttac gcattgtctc cttagatatt
     2521 tcttttccat caaaagaaaa aacagtatcg tcttctgata aatccacatg tttaggggtt
     2581 tcttttttat ctcttcctag aagatagtct atagaaacac caaaataatc tgatactttt
     2641 gccaaacctt cggagttagg tgatactgat ttccatttac tgaaatatcc atttgaatat
     2701 cccaatcttt tttctaattc ccttacggat attccgtttt gctttgttaa ttcttttatt
     2761 ttttcgtatg gattcattga tatgtcaacc tttctagact gacaagaaaa aatatagaat
     2821 aaaactctat ttatctgttg acaaattaga gttaaacctc tatacttgtt cttgtaaaca
     2881 aatttaacaa ctaaaaagac aacaaaaaac acttttgatt attaaacgcc aaccgccaaa
     2941 aaagctttta aaatcaatgt ttatatgtct tatttaacta tgtacaaagt atagaataaa
     3001 actctatgct ttgtcaattg aatttagaaa aaagttgtta aatttgttta cgaatataaa
     3061 agaaaggaat gagaaatatg aacacaccgc aaatttttaa tttcgaacaa aacgaagttc
     3121 ggacaatttt agtaaatgat gaaccatatt ttgtaggcaa agacgttgcg agtgttttgg
     3181 gttattcaaa cactaaagat gctttgtcgc ggcatgtaga tttggaagat aagatggggt
     3241 cgcgaatcac gacctcaggt caatcaagag agatgacaat catcaacgaa tctggtttgt
     3301 acagtttaat cttaaaatca aaacttccct ctgccaaaaa atttaaacgt tgggtaacaa
     3361 gtgaagtgtt gccagcaatt agaaaacatg gaggttatct aactccagaa aaagtagaag
     3421 aagctttgct taatccagat acaatcattc aattagcaac tcaactaaaa gaagaaagaa
     3481 ctggaagatt aatcgcagaa caaaagattg cagagtacga acctaaaatc tcctatttag
     3541 atagcatatt atcttctaca gattcagtaa caattagtca gattgcagca gattatggga
     3601 tgtctccaca acagatgaat aaattacttc ataaactagg tattcagaaa aaagtcggta
     3661 accaatggtt attgtgcaaa aaacacatga accaaggata cacaaaatct catacaactg
     3721 agatcccgaa agccgatggt ggcactaaaa ttgtaatgaa taccaaatgg acacagaaag
     3781 ggcgtctatt tatctacgaa ttactaaaaa aagaaggata ttaccctcaa atggatttag
     3841 aggaaattgg ttagaaagga gttttagtat gactgacatt gcagaaatca ctcaacgaga
     3901 tagagaaaaa atcaaagaat atgtcgaaag ttcaaagttc ttaacttaca ccatgctcgc
     3961 tgaaagattc ggcattagca aaagctactt atctttaatt ttaaacggta aaaagacttc
     4021 tgcagaagca aatagaatca tagattcgat tattactatg tacgaattgt aaagggagga
     4081 acagctaatg caatatctag aagcgaaaat cccagttcca gaaggctatg taattatctc
     4141 ccaagtggat tatgaggagt taaaaaaagc tgatgatact ggtagatgga tgacgttgcc
     4201 agaagtgcta gaacggatta acagaaaata tgattggttt acttctagag ttttaaagaa
     4261 cccaagatat agaaatatta tcgatatcga aaaaaacaaa aatggatttg tctattatcc
     4321 agttgaagga agagacacat atctattttt aagaagtaaa acacttgaat ttttagaaac
     4381 aaatttttcg gaaatcttaa ggaggcaagc ggatgggaaa atttaataga gcgctagtat
     4441 tcagcgcacc gctaatcatc tacgctttag gactttgggg aagcagacaa gcgttgatag
     4501 gaacgattgt ttacatggtt tggattttta tggggctgga tgaagctgag tacaaaacaa
     4561 aaaagccagt cgggagggac tgactaatga aaaaaagttt aatgactata aacgaaaaac
     4621 aattgaaaga aaaatttaat gatctcatca aagaatttgt aaaagaaact ggagaatttc
     4681 ctaatcaaat tcatctagtt gcggaggggc atagccggta tcaagctgtg aaatttgaga
     4741 tgaagaaaca aatctattga atttagcagt acctctactt gtaagaacat ttattgtaat
     4801 gtttttttcg actaaagatt catcgtatct aaaaaccaac gtagtaattt ttgactcata
     4861 cggtggaatg gtaataggta atgacaacgg aggaaatttt gatagatcag cacctttttt
     4921 taaagattta ccagaatacg cacgtatacc atgtggaagt tcgtcataca ttgattttac
     4981 cgttacagaa taattatcac caatcattgt aaaagcattt aaaggatcag gaattccaga
     5041 aatcgaaaac tcaataattg taacaggtaa agaactgtta tttgttaaca atacagaatc
     5101 tataatcctg aatttatttt gatggtaaac atctggtttg gaatcacttt ctctatctat
     5161 caaccattct tcgcattcag cgaagaaatc caattttaaa ttaaattttt tattttttct
     5221 atctgtatag gagatataaa aagcaaatgt tgaaaatact agagacgtaa taggcaaaat
     5281 aaaatcttta atggttttaa caatgttatt ccagtcaaat tgtttaataa agtcaataag
     5341 actcaattta taccaccacc attttttatc tacattatat caaagaggag agaagaaata
     5401 atgcaagaat tagtaatttt gaaaaataaa gaagctgtga ctacgagcat acaagtggca
     5461 gaaagctttg agaaaaaaca tcaacatgtt ttaagagata ttgatgcact aaaaaaagat
     5521 gtgtccaatt ttggacagat gttttctgaa ggtaatgagc cagattccta cggaagaaat
     5581 cgacgagttt tcttcattag cagagatgga tttttcttgt tagctatggg atttacagga
     5641 aagaaagcta tttacttcaa acaaaaatac attgaagcat tcaacgaaat ggaagatgtt
     5701 attcgcaaga atactgttcc tcaaacaatt gaagacatga tgatctacca actagaagaa
     5761 atgaaagatg ttaaaaaaga tgtctccatg cttaaagata ctatgcgaat tagcggacaa
     5821 caagagtttg aaattaagca aaaaggaaat acgaaagtta tggaagttct agggggaaaa
     5881 gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg gtctgaattt
     5941 aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt cgatgatgct
     6001 gtttcattta ttgaaatgtg gttaccagaa actgcgattc gcatggaaat cgatcaactg
     6061 aacagacaac aaagactttt tggtgatgaa aatgaataga gctgaagcgc taagaatcgg
     6121 gacggtaatt gctaatcgct ggtggagaca caataaacca atcatcctaa gccaacaaca
     6181 tattgacaag caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc aagcaaagag
     6241 tcacaaagaa aacacatcat aaggagattt tagcatatgg aaaaagaact ttccactcta
     6301 gatcaatatt tgactgatcc tagttggggc aaatcgaata tcaaggaaac aaataatcga
     6361 aaaatcagac gaaatctttt gacggatgaa gaactagcat gtgatcaaga cgatttggga
     6421 aattttgtga gtatttggga tcatgtctat cttatccatt tatcgaggaa gtccaaaaaa
     6481 cctgaatata tctatgtcat cgaagatggc ttgattgatg cgctagaaga gtatgacaga
     6541 gataacttga ttgatatctc ttattacgga tcaggtaaga aatacattgc tgaaatggag
     6601 gcagaatttg atgagtgaaa acaaagggac aacgactttt gaaaaacttt ttagtcgcaa
     6661 gttaaataaa atactcaaga aaaaaggaaa ttttgattat ttatcttggg ctcacgcatg
     6721 ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt ataaacacta
     6781 cagagttgtt tctggaactc atcaagactt tcttgttgag gaatataaac cttttcttat
     6841 ggatgaaact gggacttatg tatctgtctc agtaacggtt aaaggacaca cggaaaccga
     6901 attatttcct gttttagatt atcgaaacca accagttgtt aaaccaaatg caatgcaaat
     6961 caataactca ttgaagcgat gctttgtgaa agcattggct ctacacggac tgggattata
     7021 tgtatttcaa ggggaagata ttccaacacc acctagaatc gatacgaaga aattaaacat
     7081 gctagagacg attctagaag ctttcaatga gcagatgggt aaagatatga ccaaaacctt
     7141 aatcgaatat gttaatgagc agacagataa attagggctc ttagctgata acgttgaaac
     7201 tattgaacag ttaagctatg agcaatgtgc cttgatggag cgagcaatag cagctaagag
     7261 aaaagaatta gataagaagt gatatgagtg tttaaaccat taatcgattc atattcagcg
     7321 gttctgaaaa agttcaaagg aaaagacata ggtgcaacca tcaatgagga agtgaacatt
     7381 gatcgactaa agacgatgta tgacggctac gatggtgatc gagttattga aattcgtttt
     7441 attgatccta gacgtttcac agtacagcaa cgaaacttca tctatgcact cataggcgat
     7501 attttcatcg atacaggcat gccaacggac ttctggaagg aattctttta cttccgcttt
     7561 gaaggtgtca cagggcgcaa aataagcctc aaagatgaat cgaatacgac tgtgagtgat
     7621 gctaacgtct tagcaaatat catcttagat ttcatctttg aacatcatat tcctttcaaa
     7681 gaaggctatg agattttacc agcgaatcaa gaatattact tctacaaatg catcacaaaa
     7741 agagtctgtt gcatctgtgg caaaacagga gctgatatcg atcactttga caaagcgcta
     7801 ggaagacgaa agcgcaaaga agttgatcat tcagagtaca catttgcagc actctgcaga
     7861 atccatcaca cggagaaaca caagataggt gtgatcaatt tcaaaaataa atatcaaatc
     7921 aaagggatca agttaaacca ggaaacaatt aagaaactta gaataggagg ataaaaaata
     7981 ttggctgaca acaaacgata ctactattta aaactaaaag agaacttttt cgatagcgac
     8041 gagatggttc ttttagaaag tatgccagat ggatacattt attctaatat tcttctcaaa
     8101 ctttatttaa gaagtctgaa acatgaaggc aaactaatgt ttaatgacag gattccattc
     8161 aattctacaa tgcttgcgac tattacaaga cactctgtgg gagttgtaga aaaagcagta
     8221 caaatatttc gtgatttaca acttattgag gtattagata acggagcaat ttatatgtct
     8281 gatatacaaa gttttattgg gaaatcttca actgaagctg ataggaagag aaaatacaga
     8341 aaagaaattg aggaagcaaa acagaattta ataacaagtg gacaaatgtc ggacaaatgt
     8401 ccggacaaaa ctacaccaga gttagagata gagttagaga aagatataga gttagagaaa
     8461 gatatagaga atgtaacgcc ttcgaaaaaa tcgaaggcta agcccgtccg tcataaatac
     8521 ggagagtata aaaatgttct tttgtcagat gagcagatgg agaaactcaa aatagaattc
     8581 cctaatgact accaagagcg aatagaacgg ctatctgagt attgtgaatc atctggtaag
     8641 acttataaaa actatttggc aactattcga agctgggcaa gaaaagaaaa aagtgaatct
     8701 aagaacgcaa gcagtggata caagcgcaca ggaagacgag agaagcttcc tgagtgggca
     8761 atcgaccaag aagcctatct caagaaaaaa gcgctagaac gagctaatag acaatcaaaa
     8821 gcaccattct aagaggtggg aaattgaaaa tcgattatct agaactaatt aatgaaatag
     8881 caaagtataa aactggtgag gaaatagaaa tcctgagaga cgtatatgat caactcgaag
     8941 aagctggaat cgaaggaatt aagaatgatc gttcgagttg gagtaagctc agatactatt
     9001 tcgcacttta tatcgataca acacaattaa gaaatttagc atataccaaa ttactatttg
     9061 ttgattgtgt taaaggcttg caaaaacatc ttaatgaact tgagcaggtg taatcagatg
     9121 gacctaaaga catttacagc acagattgaa ctaatgcatc aagaagcttt aagacaaagt
     9181 gtatcgtacg aagacaagtg gctcaacacg ttccacggtg gacgtgagag cgcacttgat
     9241 caagtactca aattattgaa aggggaacgt cgggatggat aagaaagcgg cttggcgaaa
     9301 attaatgttg ctgattcaag atgagaactg gcaagaagat gaagcagtgg ttgctgaagt
     9361 tcagcgtcta gaaaagattg ctaacggacg tatacgaaaa aagcctgata agcggaaact
     9421 tcgcaaagga aaaatcatcg ttgttttgta tgaaggtaac attttgatgc aaggaacggc
     9481 aagagagttg tctgcagaaa ctgaatatac gagcggaact attcggacgt acgcgtggcg
     9541 gaatcacgtt gataaaaaag gacacgaata taagtatttg gagggcagca aatgataccg
     9601 aagtttagag cgtggtacac accatttaaa ggtaaaacaa ttggacaaga aatgaaatat
     9661 gggcaagcag gaaggttgat cactcatgct gaaatggctc cagataaata tgtgcttatg
     9721 caatccacag gactgaaaga caagaacggt gtggggatat ttgaagggga tgtagttctt
     9781 gtcaatgtaa gtaatggttt tgatcattta gttaatgaaa aaacggttgt acaagaatcc
     9841 gagtttcatt ctggattagt ttgtaaatca ttaattagtg gaatggaata tagagtattc
     9901 aaacacaaaa agatggatta tgaatatgaa gtcatcggaa atatttacga gaattcagag
     9961 ttattggagg aacagcgatg aataaacagg aaacaatcaa aaagttagaa agtattaaag
    10021 cgataggaaa tgatgcaata gctgcttgct ataacgagag tatgaatgca ggtattacgt
    10081 taatgaaaaa aatagacgaa ccgcagaaac acgttatgcc gaagtttttt gacaaatggg
    10141 caaaacaagt cctggaaaaa cgtgacaagt tttatgccat ttctcttatt gcacgtgcag
    10201 gttggggata tggtgttgat tttgaactta gcgagaacgg attatcatca gaaaacaaag
    10261 agctgttgta ctggcttgtt gataaatgca gcgacaatta tcctaataaa aagaaagcaa
    10321 tagaagcttt gttatacggc tacgaggtcg agaaagaacc gttgtatcac gtgaagctaa
    10381 aaataccagg cgtaacatac taccttatcc aaactttttt aatgccagga gatactcata
    10441 tgtgtttctc agcggctaca gaacgttatg gggctaaatg gaaaaatact tttacggaat
    10501 cagaaataaa agcaattgat gaaagatact ggccatttgc tgtgccagtg gaagaggagg
    10561 cagaaggatg aaaaaattgc accaactcat atctgagaaa gaaagcgaac tccaaaattt
    10621 agaagatagt ttgggattgg ggtttccaat tgttgagcaa gctaaaatga ctcaaatcag
    10681 tcatctacga cttgaattgg aagatttaag acaaatagaa aaatcaattc aactcaacga
    10741 caaccagcaa atcgtgtttg agtggttgaa actaactgcg ccaacaggaa agccaatgca
    10801 agttgtattt tggatgatga ataatgcagc atgggggcat ttagacgaat taagggatcc
    10861 tttgatggaa ttaacagaca aagagcaatt cgaagtccta gcagcattcg ctcaatgggg
    10921 attagaacag gaggaagcgg aatgaacaac agacatcgca gaataacgaa actaagaaaa
    10981 caggaactga atgtactaaa ggcaaagttc gaaaaagaat acggagtttc tgcagaagaa
    11041 gcatataaag tggcaagtca gtgtgttgct gatgcgagta atgctattcg taagtttgga
    11101 atttcgatat taagtgatga tcgtaaatgg gaggatatag aatgaaggtt tatgtagtaa
    11161 ggttcgggaa taaattttat agaagtgatg aacgctctat aggcaataat gtgttattcc
    11221 ttgtagattt tcttcaaaac gcaagatggt ttgataatct cgaagaagct aaccaagttt
    11281 cacgacgact tggtggatta acgcaagtat atgaactggt cactatcgat catgaggggg
    11341 tagaaagatg aaactaaaag acggatttta cgctagtagt catggtatcg gcggtttaat
    11401 gctaggtatg ccgacaaaga accctaaaac acgtaagaaa ccaaaattca aagtcggtga
    11461 catggttcgc tgcgaagcag aggagttcat ctatccgttt cgtggatatg tagagcatgt
    11521 ttataatcac tcagcgatca ttcgcattga aaacacgatg gaatgtgaca agcggttagc
    11581 gaaaagcaaa gagaatttag cagtggtgag attggtggat atggaagtta taaaatctta
    11641 attttgcttt tttacaacta tcctacaaca atgagcgtta ttccacaact ggaaggagaa
    11701 gaggcaattg tgaacatttt agagaatata gatattaagc aaacaaggaa aaatgctaga
    11761 cgattactaa aaagatacag aaatttagaa cgtttggtcg gaccagtgaa aatagacttt
    11821 tccgtgatga ctgttaccaa aaatttgaaa ttcacaattg acagtcaaaa cgaagaaatc
    11881 attgaagcga taagtactcg agattcggtt atcgaagcat taacacggct aagtagaatc
    11941 catttccagg tactttatta tagttattgt tttcctaata agatgtcaat gtatcaaata
    12001 ggagaaaaat taggatattc cgatagaact atcgagagaa tgaaggcagt agctttggtt
    12061 gaatttgctg aggcgtataa atcaggagaa ctcatttcac gtacaaaata aaaaaagtcg
    12121 gattcctccg actatgaata atatttccga cataagtatt atatcataat tgggggaatc
    12181 agaggatggt acttttcgat gtaaagaaat atgagacacc aagcgcgaaa gatgttgata
    12241 tggaacgcac aaaacataat gtcgctgtgt ttctttcagc atatctatca gctagatgta
    12301 gagtaggtca acctcgagaa ccaaaagtga cagcatctta ttccttggtt ccaccttcta
    12361 cagctgatca tatatttgaa gcagaaagaa tgctgatcga taaagaagaa gcacaggaag
    12421 aatttgagta tctgcataaa ttgtttgtcc gaggctattc agcgatacag catccgcata
    12481 agcctgatgt gactgaaagg cgcaagaaga tattctatga tcgttatatc aatggcatgt
    12541 cgatttatgt aactgcccaa agaaataata cgagcgagga atctgtaaaa gcagaatcaa
    12601 acaaaatcat cattcaattt gcttcttcct tagaactggt tgctttcaag tagccagttt
    12661 ttacactttt tatacccttt tattaccaat ttggtttcca ttttatacct tttttatacc
    12721 aatcacttac ctattcaacg ttgtattatg atagtgtcga aagattagga aacaggatcg
    12781 acaaaataaa atgtaaggga ggaaatctcc ctcatcgttt aattaagctt cgataggcag
    12841 caacggaaat attaagaata aggatgtgaa tttcaactcc ttctaaattg ttcttattat
    12901 ctatcatccg ttgctgtcta ttaatttatg tattggaggg taagaatatg aaattatcga
    12961 ttgaaggtac ttcaggagaa ataaaagaaa tcctctcagc tatttgtaat agccgagaga
    13021 ttagcaattt aatcaatata gataatttaa atgaatctat tgttactcca agttcccgag
    13081 agaaattgca gaaatctgat cagcaaaaac aactaaaaat gggatattag tagttctctc
    13141 gcctatgatg gtaacatctt tcagaaagat agatttgact accaaacttt gctcatcatc
    13201 tttttctata tcatcaattt tttttgagcg aaattctgca aaagaatcca tcatagcatc
    13261 aataagagga tttactttta cttcagcatc aaagtctata ggagttccgc aaattgttgc
    13321 tcctccagtt tgaataataa tattattgtt agaattttta gcgaagagtt caatatattc
    13381 aatcatgtct gaacggtaat catcaatttt tgccattttt ttaccacctt aaattatttc
    13441 agcggaccac tcgctgataa ataaaattat acgcttagta tttattttca caatattaat
    13501 ttatcgtaat aacttttgag taaatagaac aaaaaacctg cactagtttc cgctagtaca
    13561 ggcagtgact atatcggttg ataatctagc atatattaaa aataattgca agaaaagaaa
    13621 tttatagtgg tttataccaa attatcaaag caatgttatt ttgttgctgt ctattgtttt
    13681 ttaattattc acacgataac taaaggtggg tgaagagaaa tgattccatt aataatttca
    13741 atttttgcgc tctgtcttaa tgtctatatg attggattta aaaatgggca aaataaaaaa
    13801 tagtagcagc caagaataat tttatagtgt cactgtggcg gaaagggtag acgctaagca
    13861 tgtgtgctag gtcaatgctt cggcaaccat gcaaggttcg attccttgcc agtgactttg
    13921 gcaaccgagg catcggcggt ttaaaaatat aggggtgcgc aatttcgtac gcgttttgtg
    13981 catcgtgtag gttgctatta cataactggt taggttagat tgagatttgg gattcggtac
    14041 aaatgaatcg tcaaatgtct caagcacagg atcggaaacg tccctgcctg tgcattacat
    14101 attagatcac tcgttgagtg gtctttttat tttgcaaaaa ggaggtaaca acaatgtata
    14161 gaccacaata cttagaacag aagcatgaag taatcactgt gcaaaacggt aacggtgaga
    14221 tagtacgaaa gtatagaaga ccaataaaga gcaatacata taaacgaaag gaaagcaatg
    14281 aagttattcc attgtatggc aaaagaatag ctaagtatta aataagattg cgaaaggaga
    14341 tgggacatga ccgaggaatt ctatagatgg ttattacagt tgataagaga agatcgtttg
    14401 gttaagttct atcagtctcc taaatggcgc aggcttagag agaaagcgat gaaacgagat
    14461 cactatgaat gccaagagtg tagaagacta ggtaagtatc atagagtaga gaacgttcat
    14521 catataaagg aagtcaagga tagacctgac ttagctttag atttagataa tcttatttgt
    14581 ttatgtgttg aacatcataa tgaagttcat ggcagatatc ttacagcatt agataaacaa
    14641 gagaagaaga tagaaagctt tgctaacttc gatgcaagtg aaaggtggta agtgcatgat
    14701 catcaatgac aatggcagag agtatgacac agaaaagatt gaagagtatt catcttatac
    14761 tcaaggatta attaaacgtt tgatatacgt tcgctatgta ggtattaggg atctgttatc
    14821 agataactgt tgcagtaaat acaaagtgaa tcaagtaaga gaagcgttga ataaagataa
    14881 taacgttgaa agaataaaaa atatttttgg atatagcatt gaagagatta attattacat
    14941 tgacttcgct gaagctttca ttccgatggt gagataaccc ccccttaaaa taaatcgcaa
    15001 attttttggg ggtgatgaaa cggagggggc tgtcaggaaa agagattttt tcgaacttta
    15061 tcatgaaagg agggctaaaa tgtttaaaaa cgaattgtct caaaatcgct acagagaaaa
    15121 attacgccgc tctttaataa gccaattgga aagtcagaaa acaaatattg agccattctt
    15181 agataatgtt gatcgttata tcagtttatg ggaaacggcg atatcactgg aagaagatat
    15241 atccgagaac ggcattagac tggagaatgg taaaaagaat gaatcagtag cgttgcttgt
    15301 ttctgtcaac aaacaaatgg gattgatgtt ggataaactt gccattactc ctgaattggt
    15361 aggtgaagca aatgaatcaa ttcctgagtt ataagcatat tgaaaattgg ttcaaagcta
    15421 tagaagaagg cactatcaag gtatgcaaag agcaattatt gctaaaaaat tatctagaag
    15481 aaagagtctt tactagagaa gatatttact tcgataagca gatggtagag gattcaatca
    15541 atataccagc acaatacttt ccattcgaat taattccgtg ggaaaaattt ctacaatgtt
    15601 ttatttatgg tgttcgatgg aaaaaagata aaacgctagt gttcaataga tatctttcat
    15661 taatgggacg tggtaatggt aaaactggtt ttgcttcttg gaacaatttt ttcttgctga
    15721 ctgcaaaaca cggtattaaa aattatgata ttgatatcta tgccaataac gaaagccaag
    15781 caaagactag ttttgatgat gtatttaaag taattaaaga tcatcctggt ttagataaaa
    15841 aagtatttaa agctacgaag gaagttattc aaaatatcgc tacaaacagc aaacttcgtt
    15901 ataacacggc aaacgctaga acaaaagatg gtaaacgacc aggagcaaac cgctttgatg
    15961 aaattcacga aaatgaagat tattcaatga taaatgtagc tacttctggt ggtggtaaaa
    16021 ttcgagatta tagagaattt tatgatacaa ctaatggtca tgttcgtggt ggtccgcttg
    16081 atgacattat agaagaatca aaaatgattc tttctggaga acttggaatt gacaaggatg
    16141 gagcagaatt ttctagtttg tttccattta tttgtcgctt ggataatgat aatgaagttg
    16201 atgatcccga tatgtgggaa aaagcttgtc caactattaa ttacaatgca gatttaaaac
    16261 ggaaaatgtt tcaagaatac tctcaaatgc aacgtaatgc tggtttaaga cttacgttca
    16321 tgacgaaacg aatgaataga ccaatggaag atacgcgatt tgctgtagct tcatatgatg
    16381 atgttcttca tacgaaagaa aaagaatttc ctgaaaaaat ggatgaagtg ataggaacag
    16441 ttgattttgc tgatagacga gattttgcca gcgttggttt gttaggaaaa tatgataaag
    16501 atgtgtattt tacacaacat acttttatcc acgaatcagc tcttcgatta cagaacataa
    16561 aacgagaggt tatagatatt tctatagatc aaggaaaatc acagatcgtt catgggaaaa
    16621 atatagaagc tgattatatt gtaggttggt ttcttgaaat gagtaataaa tattacatta
    16681 aaaaaatcgc tatggatatg taccgtgcaa aaatattgaa gcccgcttta gaagaagcag
    16741 gttttactgt agaaattgtt cgaagcggat ctgttacaca tggtatgtta aaagacctag
    16801 ttgatgacct ttttattaat caacgtttat tttttggtga cgatgcgatt atgcgttggt
    16861 attgcatgaa tgtatatgaa gagcatattt ctaatggaaa tatacgctat gaaaaaatag
    16921 aacctgaaac tagaaaaacg gatggctttt tttcattcct tcatggtttg aattttttag
    16981 atgatattta tgattctgct cctgtaacag tcacaaatag ttcagtagaa aatgcaggaa
    17041 ctggatttac tcctctagta ttctaacttg aaaggaggtg agaaagtggg gatttttcaa
    17101 aaggcggtag gatacttcac aaaaaaagca acggttcctt tagaagaata cttttgtaaa
    17161 ttgcaagttg attttgtgta tcgaaaattt gcaattgaaa cttgtattga tttgattgca
    17221 aatgcgatga gcaaagcgga attcaagtca tatgaagatg ggaaaaataa aaagaatgat
    17281 ctttactata ggctgaatgt agctcctaat aagaaaaata atgcaacaga atttagaaaa
    17341 aaactgatca ggagattaat attctacaat gaagtattga tcgtttctcc gtctaataat
    17401 tctagcgaaa tatttattgc ggatagttgg gatgtcacag agtatgcatt aaaagatgat
    17461 gtgttttctc aagtgcaaat taacaacata gtccttgata gagaatttct agaaagtgat
    17521 gttatctata taaaatacgc agatcaacaa attaggcaac tagtcgatgc gtattatcaa
    17581 gcgtatggga aactcatttc tagtgccatg aatgtttaca agcgttctaa cgctcgtaga
    17641 tacgtactga aagggaattt attccgagcg caagacaata caacacaaga tcaaatcaat
    17701 aaaatgatga catcacaatt taaggctttt atggaagctg ataatgcagg tgcggtattt
    17761 caattacaaa atgagtacaa attagaagat ttcagcggaa actttcaaag caattcaaga
    17821 gatataaaaa acttaataga cgacatcttt gagatgacag cagcagcgtt tcacgttccg
    17881 aaaaacctac taaagggaga catgagtgga ttatcggatc aagtggacgc ttttttaatg
    17941 tttgaaatca taccgattgc tgaacttatt caggatgcgt ttaacgctag tctctatgaa
    18001 gtagaagaat acttgtcagg gaattttgta cgtgtggata caactatgat caagattact
    18061 agcttcaaag atttggttga cgctattgat gtaggcatta gaaatggggt atttacaatc
    18121 aacgaaggaa gagaacgcgt tggaaatgat cgctctgata aggcgatggc agatgaaata
    18181 tttataacta aaaacaatca acaagtatcg aaaggaggtg aggcgaatga cgacaatgaa
    18241 aacatttcta gcagtaaaga atgaaggcgc agtaccgcaa atttttattc agggatttat
    18301 tggttctagt tggttctttg aagggaatac tgacaaggga atcaaaaata ttttggatag
    18361 tctaggtgat caagaagaga ttgaagtagt aattaattca aacggtggag acgtatttca
    18421 agggattgct attgggaact tacttaagtc aaataaagca aaagttaacg ttgtgattaa
    18481 cggcttagcc gctagtgctg cttcaattat cgcaatggct ggcgatacta taaaaatcta
    18541 caacaatgca caattgatga ttcaccgcgc ttccacatac ggagaaggaa atgtcgatga
    18601 cttccgtacg attgctgacc aactggaatc aattgataaa tcggtaaagg cttcatataa
    18661 aacacgattc aatggcacag atgaagcatt gcaagaactt cttgaaaaag aatcgtttat
    18721 ggatgcagaa acagctttga gttatggatt ggtcgatgaa attatcgatg cagaaaatag
    18781 ctcaggtact gaagctaaaa aagaacaaag cgttgaagaa attttgaatg acgttaaaga
    18841 aaaaagagaa gaaaaaattg ctgcatttac agcagcatta aataaaacat ttggacaagg
    18901 agatgtaaaa taatgacagt taaaaattta aaaggtgtaa cagctgcaag cgaccaattg
    18961 atgaaagctt ttaaagatgg taacgaagaa tcttttagcg cagctatggt aagcttatct
    19021 aaggaaattc aggataaaat tttagaagaa gcaacagcaa aaaatcaaga tcaattagta
    19081 ttaatgaacc gtggtcagcg tgtattaact acacaagaaa caaaattcta taacgaagta
    19141 gtgaaaaacg aaggttttgc aggggttgaa gaattagtgc cagctactgt atttgaacgt
    19201 gtatttgaag atttagaaca atctcatcca ctattgcaaa aaattacttt tgttaacaca
    19261 actggtgtaa cagaatggat tgtgtcacgt ggagtcaatc cagcatggtg gggtaaactt
    19321 tgcgaagctg ttaaaaaagt tttagataat ggctttgatg taattaacat gaagcagttc
    19381 aagctatcag gttatattcc tgtatgtaag gcaatgcttg atttaggtcc agtatggtta
    19441 gatcgttatg tccgtactgt tttagtagaa tcattgagaa ttgcattaga acaagcaatt
    19501 gttgatggta ctggtaaaga tatgccagtc ggaatgatgc gtgacatgag caaacaaact
    19561 agcggagaat atgctgaaaa aaaagcagaa cctattacag ctttagatgc tgtaactatg
    19621 ggcggtttga tggcgcgact atcaaaattc aatatcgaag gtgtgaatga tccgatttat
    19681 cgtaatgtga atccttctga tgtggtccta attgtgaatc caactgatta ctggtctaaa
    19741 gtattcccag ctaagactgt actaactgct aatggagaat acgtacaagt attgccagta
    19801 ccagtttcag atttgcagtc aacggctgtg ccagaaggaa aagcagttat tggggtagcc
    19861 tcagattact tcatgggtgt aggatctaca ctaaaaattg aagcttcaga tgaataccat
    19921 tttgttgaag acgaacgcat ttatctagct aaacaatatg caaacggtca acctaaacgt
    19981 aacgatagtt tcattgtatt agatattagc gctttgggaa ctactactac aactacaaaa
    20041 ccaacaacca caacaactac aacacgagcg taggtgatca gaatgaagta tattctttgt
    20101 cagccggcaa tcaatcggtt taaatgggag cttgaagttt gtttaactaa tctgaagaaa
    20161 ctaggaatca aagatatcgt attgcttttc agcagacacg atgatcagat tcctattttt
    20221 tttgagaagg aatatggtgt tgaagttcat gtgtacgatg atctgcggga cgacaaagag
    20281 tatattcctt cgattaaacc atatttatgg tggaaatatt tagaagaaga tcattcgcgt
    20341 gaggacgacc gatatttcta tatcgattcg gatgtcattt tcaataaaag aattaatttg
    20401 cgcaaattgc cttctaaaga tgatgtttgg tattgtagcg actgctgtag ttatctaagt
    20461 cttgattata ttagaagctg tgaaaacgga gaaaatattc taaaagatat ggcaaacatt
    20521 gtaaatgtta cagtagaatc tttggaaact ataaacacta attcaggagg cgcacagtgg
    20581 gttattaacc gtcctaaagc gaattattgg aaaaaggttt atctggattc taatcggcta
    20641 tatcgctacc ttagagggca aaaaacaaat atacaaatct ggacagccga gatgtgggca
    20701 cagctttgga acatgatgta tttcaatatt ggtcctaaag ttcacgagga attagacttt
    20761 tgttttgcta ctgatccaat agaaaaagtt aaagaagtaa aaatcttaca caatgctgga
    20821 gtaacaacaa acgatgaaga tttatttttc aaagggagat acgtgacttc tacgcctttt
    20881 gatgaagatt tatcatttgt aaacaagaaa aaatgctctt acgcatatgt taaagcaatt
    20941 aaggcggtgg ttagatgacg cctgaacaag tgactgaaga attgctaata gctgtgaagg
    21001 ataatattta cgttacctgg aacgaagaag atgagtcaat taaaaagatg atagctaaaa
    21061 atgctgttta tcttcaaagt aaagtgagta caacactttc tttttctcct gaaagcttag
    21121 aatacggatt gctaatcgaa agatgtagat acgactggaa tcgtgcttta gatgagtttg
    21181 aacaaaattt cgctagtgag ttattaggtt tcattcaaca ttatgcgcta caagaatata
    21241 ttgcaggtga tgtgaatggc gaataatcgt agactcgaag aaacattcaa cgatggttgg
    21301 ttaaagattt tgacgcaaac taccaaaaga aatgaactag gaaaaaagat tggtgtagaa
    21361 gatacagaaa tcacttcttt aaaatttaga aatctttcca tgagagatag tgatataaca
    21421 gctatggatg cgatgggatc gaaattaact aagaaagtaa agactccatt tcatccaatc
    21481 gccaagaaat ttaataaaga tcaatatttt atcgtaatcg atagtatgcg ttacaacgtt
    21541 atctatgccg attacgataa tttttatatc tatttttatc ttgaaagtgt gggtgaatat
    21601 ggtgattgat aattctaaag aaaaagaacg tttaaataag caaatttctg ctatcaaaac
    21661 ttccttagaa gagcattttg gcctcaaact ctttcaagac tccgtaggcg aggatgagct
    21721 acctgatgat tttaattact tcattctcga aacaggagaa atagaaatga tcactgagcc
    21781 aaaatatagc gtgggtcaaa atctatatct aactttctat tcagaaaata gagaagattt
    21841 aacaggagac tcactagata ttatttcatt gattcaaaat cgttcgattc gttttcagag
    21901 aatggatccc aatcatttaa aactagagaa ccaagatcgc tatatcgatc aattggtatt
    21961 tacgtttaga cgattattga agagtgattg tcatggctaa aaatagttgg gagctaaaaa
    22021 taaatggaca tgatgaactt cttgtgcgga tggaacgcta ttcaagcgag agcgaacgac
    22081 tgattaacga agcattgaaa tcaaaaggtt cggctattgc agtggatagg attacagaaa
    22141 aaattcctgt ttctgaagca gatttaagaa gagggcacca acacgcaaaa aatagtcgtc
    22201 cacttaagac tcaatacatt aatttgggtt tcatcattag acctacaaga aaatttgagt
    22261 atttaaaata tcctgatttg gggataggta cttctaaaag aaatcagccg gacgaattta
    22321 tgagaagagg attaggtctt gcacttgatc caattacaga acttctgatt cgtcaattcg
    22381 ataaattaaa taaatagggg gaacaacaat ggctaaaaca aaaactgtag taacaacgtt
    22441 cgataacgta agtatcaaac gaattgcttt taattttaag aacgcaggaa atgcaatcgc
    22501 aacgaattgt aacggacaat tagatggcga aacagaaatg caaacggtgg ttaaaaaatg
    22561 tggagcgaca gaagtaaaat caaaatctaa accaatcaat atgacggtaa caattactgc
    22621 acatgtaccg atggaagttt atcgacgttt caatgggttg aaacaagatg gacgtattaa
    22681 accaggcatt tactcttacg gtcctgattc cgtaggcgaa gatttctcac ttgctgcaga
    22741 gatcgtggat gacttcgaag aaaatagaaa gttagttggt atgttagcat gcacttcgaa
    22801 tacaggatta acattctcta ttgaaaatgg tgcggatgaa gtagctgcgt tagaactaga
    22861 aacaaaagtt atgcaagatg aatttggtaa attttatcat gaagcaattg ttgcagaact
    22921 tgaagaagac ttaacagatc aatggatgac gaatctatct gctgatgtga ttaaaaaaac
    22981 ttcagttgtg acaactacgg ccactcaatc acagtaaaaa aaacggaggt agcgaaatga
    23041 acgaagatta ctcaaaaatt gaactaaacg atggaacaat tttgaattta gaacctaaac
    23101 tgaatatcaa gaaattattg atgatcaata gagattttaa cacagacgag tttgcaaaaa
    23161 tgactgtggg aaaaggatcc atggatattt ctgttattca aggtgcaaag gctgtgtata
    23221 ttgcttaccg ccaagcgaac atgactgatt atatttcatt cgatgaattt atcgataaat
    23281 gggattttga tatggctact gccagctata tttatcaatt gatgatgttc aaacaagcac
    23341 gcgatgctta tcaaaaagaa tttgaaaaag caaataagga aaaaaagctt caaaagtaaa
    23401 aatgccaaag ctcttagttg aaacgtgggt cgatgtctat tcgatgttga ccgacgtttt
    23461 ttctatgcct tcagatttgg ttttaagcga tatctgttta gatgacattt tgcaaatggc
    23521 ttacaacaag agtgcttatg aaggatggaa aaactatgca ataaaccaat ctcagaaaaa
    23581 ctaaagaaag gaggtaaaaa atggctaaaa agagaacaga agcagaagta actttcatag
    23641 ctaacgatga cggattgaaa tctacgttaa aagaaatcag tgctgaatta actaaaaata
    23701 gagcagaatt aaaactagaa caagctcaat tacaacagac tggttctgaa tcagacaagt
    23761 taggaagtaa attatcttct ttagaaaagc agtatgaatt acaaagccaa aaagttgaag
    23821 taactagcca acgtttagcc aatgccaaaa aatattatgg agaaaattcc accgaagttc
    23881 agaaacttga gagagaactg attaaccaac aaacagcgca acaacgtttg tcaaacgaaa
    23941 ttgataaaac gagtaatgca ctagctcaag caaaaggcga aatacagacg tacgagtcta
    24001 caatgcaaca gttggatagt gaacaaaaaa atgttcaagc tagtgcttct ctgattgaat
    24061 ccgaatacaa aaaatggcaa gtaactgctg gtcaatcagc ttctgaatcc gagaaattag
    24121 cgaaagccca agaatatgtt tctcaacaat ctgaaaatgc agagaaaacg atagatatcc
    24181 tgagacgaca gttagaagct acacagtctg agtttggcgc tacatccaca gaagcaatgc
    24241 agatggaggc gaagcttaat gatgctgaac gtgaatttga agagttagga caagctgcta
    24301 aaaatgtaga tacaactaac ttggacgata tcggaagcaa aatagatatg aataatttaa
    24361 tggaagcttc tgacgtttta agcgacattg gcgataagct tacagaatta gggaaacaag
    24421 cagtggactc agctaacagt gtaggtagtt cccagagtaa gatacaagct aattttggtt
    24481 tgactaaaca agaggctgaa gaattaacga atgtagccag agacatttat tataaaggtt
    24541 ttggagaatc gttagatcag tccacagatg cattgatttt ggtaaagcgt aatttaggcg
    24601 atttaaataa tcaagattta caaaatatca cggaacaagc tatggtccta gaaaacacca
    24661 tgggcgctga tatggatgaa acgttacgtg gtgtaaatgg cttaatggtc aatttcggct
    24721 tgagtgctca agatgcaatg gatttaatgg tttcgggtac tcaaaacggt ttagataaaa
    24781 cgcacgaatt aggcgacaat atggcagaat atagccaatt atggagtcaa atgggatatt
    24841 cagctgatga aacgttcgga atgcttcaaa atggtttaga tgcgggtgct tataaccttg
    24901 ataaagtcaa tgacttagtt aaggaaatgg gaatatcgtt aacagatggt cgatttgagc
    24961 aaaacatgga tatgtttagt gaaagtacta gaaaagcttt tgaagagtgg aaaaatggcg
    25021 gaggaacaca aaaagacgtt attaattcca tgattcaaga ttttagcaat atggatggtc
    25081 aatacgacca attaaataaa gcttctacaa tttggtctgc acttggcgaa gataacgcga
    25141 tgaaagttgt ccaatctttg actgatgtta accatacatt tgatgatgtt agtggatctg
    25201 cacaaaaaat gaatgaagat tctactactc cgttacaaga attaaacgga aaaatagctg
    25261 aattaaagga ttcattagct cctataggca acacaatcat agatgcactc gaaccagtaa
    25321 ttgattttct aggaaagatg gctgatgcgt ttaataatct tccacaacca gtacaggatt
    25381 atgccgtagc gattggcgga ttgactgctg catttacttt attaatgcca ataatagttg
    25441 gcttcatggc tctaggtggt cctactacat taataatagg agcagttatt actgctattg
    25501 ctggagttat agcaattata aaaaactggg gcgcaattac tgactggttt aagggaatat
    25561 ggagtaaatt cactgattgg ttgggtggta cttgggaaag cataaaagaa ggtgcctcat
    25621 cagtttggga tggagtcaaa gaaacctggt ctggatttgt agattgggtt caagaaattt
    25681 ggcaaggagt ttctgattgg tttggagagt tatggagcgg attagttgaa ggagcttcca
    25741 acatctggca aggagtccaa gagacttggc aaacattcat tgattgggtt tcaaatattt
    25801 ggaacggagt caaagaagta tggtcgatta tttgggcaga cattgtagga attgttcaaa
    25861 taccatggac attaataacg tcattgattc aagctggtat taatattatc gtgggtattt
    25921 ttgatgtagc tggacagtta ttaggcgcag cttggcaagc tgtttggaca cctatttctg
    25981 atttccttaa aaatacttgg gatactatga cacaatggat aagcatcgct tggaatggaa
    26041 ttgtaactac attccatact atatttgatc cagtagtggc atggtggaat ggtatatgga
    26101 catctattag tactacggct tcaaatattt ggaactcaat tagtgcaaca gcttctagta
    26161 tttggaatag tatcaagaat acaatcacta gcttggtaca agcagttgct acagtaattc
    26221 aaaatatttg gtcaactgta tctagttggt taggtggaat ttggaattca atcagctcta
    26281 cagcatcaaa tatctggagt agcgtgacta gtagtataag caatgctata aacgcagcta
    26341 aaagtgccat tcaaagtgtt tggaatagta tatcttcgtg gatcagcgga atttggaacg
    26401 gtatcaaaaa cactgctttg aatctttgga atggaattac aagcactatt agctctaaag
    26461 taaacgatgg aaaaaatgca atttcaagcg gttggtctaa tctaacaggt attgtttccg
    26521 acatattcaa taatgttaaa agcacgatag ctaatatatg ggaaggtatc aaaaagactg
    26581 ttagcgctcc gattgattgg attagagata aaatcagtag tgtctttgat aatttgaata
    26641 tttctatacc acatattccg ttaccacatt ttaaattgag cggagaattc aatccattga
    26701 aggggaaaat cccaacattg ggtgttgatt ggtatgcgaa aggtagtgtg tttaattctc
    26761 caaatattat cggtgtcggt gaagcaggac ctgaagcagt tttacctttg aaaagatctg
    26821 tgctgcaaga aattggggat cgtatcttga gtagcacctc agtttcatct agggcacaaa
    26881 cgattcaacc tgtgaacaat tacgaattca atttcacaat tgatggtaac gcagatgaag
    26941 tcactatgaa gcaaacaact caacaaatca ttgatagcat tacaaaagtt caaaatgata
    27001 atgcttcggc atggcgttaa acaggagagt atttctcctg tttttttagt tttaaaaagg
    27061 atgtgaaaaa atgactgatt gtatacattc tataatcgat ggatttcctg attatttgca
    27121 taaattggct ttagcggaaa gaccaaccat accttctcca aaaagacaga gagttgaaac
    27181 ttctgtttta ggacggctag gtggcttagt acaagattac tcgtttgaag acatgtcgtt
    27241 tacattgcac tataactatt tagaagatgt ggaagaccat caagcattca agcaatcgtt
    27301 ttatatcatg cgtcattggt taaactatgc aaagaaatta gaattctctg atgatcccaa
    27361 cgtctattat gttatccaga ctatcgatat tggggatgca gaaaacgata ttgttgaatg
    27421 gggagagttc gatgtaaata tcactgcgaa accattcgca agagttcaag aagatgtacc
    27481 tataaccgta gataaaccac agtcatttaa cttgctgaat aatagtttag aagaaagttt
    27541 tccaaagatt atcatcactc cttcagctac ttcatgccag ttcatcttaa atgattatgt
    27601 gtttagtttt gaaggcttag tagtaggaac tgatgtagtc attgatagtg atttaatgct
    27661 ttgctacgaa gagcaatcgg acggagatat tttagatcgg tccaacaaaa tgaagaccat
    27721 gcaatatcca acgttgcaag tggatattaa tcattttact tgtactggtt tgagcaaaat
    27781 acaaatttat cgtaatgggt taaggtaggt gaaatagatg atcgataatt tactaactat
    27841 ttacgataaa aatgacgcga ataatttagc tgaacattta tatgatacgc aaggtttagg
    27901 cgctttgtca gactggttaa cagctactgt tagcaataaa ctaaacggag ccgagatatt
    27961 tcagggtact tattcaataa acggaactaa tgcagatttg attgtagaag gacgtattat
    28021 tcagtgttat gtagatgaaa atcgagcaaa acagcgtcta cggatttatt atgcaaaaac
    28081 ttctgtaata ggcaatacga tagaagtaaa agctgaacct attttcaatg atataagaaa
    28141 atcggtgttg aataaatatg acagcggaac agaaaaaatc actgctactc aggcatggca
    28201 aaacgcaaaa gttttagcga aaccagctat cccttcgcag ttttctttct cgtcattagt
    28261 agatacgctt gctaatgtga agatagaaaa ggcgaatttt ttagaattct ttggtggaaa
    28321 agagggatct attctagatc gatttcatgg tgagtttcta aaagataata acacattacg
    28381 tcatgaaaaa agtctaggta cggatcataa aatcaaagcg atttatacta aaaacttaac
    28441 tggtcttgac ttagagattg atgctcaaag tgttttagtt ggagtttatc catttattag
    28501 cagctcttca gaaggagaag acgagatcac tctaccagaa gaagttattt tcacggatta
    28561 cgtggatgat tatcctgctg gatatgtttc ttttgttgat tttaaagaca aagcgactga
    28621 tgtagccaca ttaagggaag ctgctaaaga ctggttgaaa acaaacatag ataaacaaaa
    28681 accacaagtg agtggttcga ttgaattagt accattgagg catcaaagag gctatgaaaa
    28741 atttgttgat ctagaaaaag tttcgatggg tgacggagta gatgtgtatc atccacagtt
    28801 aaaagtgaat atgtcagcga gaattgtgga atatacgttt aatgttctaa ccaattcata
    28861 cgataaatta gttgtaggaa acgtcaaaac aaacttctta gaaaacacag agaataatgt
    28921 cagcaatttg attaatgatg ccattgatca attgaaaaac gttggcgaaa tcagtgattt
    28981 aatcaatgat attgtagatc atcaaactga tatgattact ggtcaaaatg gtggttatgt
    29041 tttattagat cctaaagaag cgcctagtcg tattttgatt atggacacac cagataagaa
    29101 taccgcaagg aatgttttac aaatcaacaa tgctggtatt ggtttttcta aaactggcat
    29161 taatggaaca tatgaaacgg catggacgtt agatggcgga ttcaatgcct cgtttattac
    29221 ggctggtgaa atagtaggaa ttactattag aggtactaca ttaattagtg atggtgctga
    29281 ttatagaaca agtattgcta atggcaaaat gacttggtac tcaaaaaaag ttaacaaaga
    29341 tattatggag ctagaagcac gtgattatgt aagtgctgat gccggtattg tatcatacac
    29401 catgaaaact ggtggtggtt tcatgattag aaatccacag ggtaacttgg tttttagtac
    29461 gtgggataat ggtaataaca gaccgtttct atcttttggt gcgcccaatt tcaggtatag
    29521 caatgctagt tatgtaactt ctggcgacgg tagttcttta agcattaatg gtagtgcggg
    29581 taactcatgg gaatttaagg tagctggtag gactatgaaa tttactagtg atggtatgct
    29641 aacgttacca ggttgttttt ttggttcatg ggaagatggg aaacttgcta ggtttgaaca
    29701 atcaacggta caagtatata aagattttac tgttagaggt actaaaaact caactgtacc
    29761 aacagaacat tatggacaac gactattgaa cgcttatgaa actccagaat attatttcgc
    29821 tgattatggg gaagccgtta caggtgacga tggtaaagtt cgtgttgata ttgaccccat
    29881 gtttgctgag acagtaaatc taagtcggta tatgacacat gtgacaccta cagaactagt
    29941 tttgtgtgct gttactcatg aagatattga ccatttcatc attgaaacta gtaagccaaa
    30001 cgtattagtt agatggaatt tagtggcaca ccgtctaggg tatgaagata ttagattaaa
    30061 agaggataca gcatatgata gcacagtgct tgaccaaaaa cgtttttaaa acgaagacaa
    30121 ggaggtatat aaatggctag cagtttatat aatttggctt tagatttcag caaagaatta
    30181 aactacacca aagctattat ggctcgtcag ggtgataaag ggattacggt gacggttaaa
    30241 ccatatttaa atggcttgca gatggatacg agtggcggaa catttacttt aaaaggaaca
    30301 acaccatcta accgttacgt agataatgtt gcaactagcg taactagtga agaagtcacg
    30361 ttttctcttg atggcacatt tatgagtgaa gcaggatatt ataaacactg ctacgtagaa
    30421 tatagaaaag acaatcaaat tctaacaacg caagatatca tttttttctc actaggagtg
    30481 tctgacattt cgcaaggtca agccgatgaa tatgtttcgc aattagaaga gttgattcga
    30541 aagtataatg aaacttttga tgcttttatg gctgaaacta aaggtagagt ggatagctta
    30601 aatcaacaga ttactgattt aactggtcaa gctaaaacgc tacaagacaa gttagatgct
    30661 ctgaaagaag aaatttctaa gttaggtaac ttacaagtga tgtacagtaa cagcatcgat
    30721 ttcgggaact atgattatag tgggaatcct aatttgttaa gtaagctatc atacgactta
    30781 attgaaaatc aaaatacttc agctggaaca ctttctaaag gtgaaaactc gtttaaatat
    30841 aataagatat cagctgaaac ggaaggtgga gtagagttat attataaacg aagaggtata
    30901 gctaactggt taccctctaa taaaacgctt gtaatgaccg ttaaacttag agctggagtt
    30961 gactatagtc cagctgacgg aaaaaaaata ctgattagat ataggtttgt tgacaatgga
    31021 actggcaaga ttgttttaga cttacctatt aacagtaatt cgataactca ggaatggaaa
    31081 gagtttagta ttactggaac tactccaaca tttggcccgc aagcatacca tccttggata
    31141 caatttaggg ctcaagatgg gatacttggg gaaatagaaa tgagctatga catcaaaatc
    31201 gaagaaggtc caacagccac accgttccaa cctaacttat tggcagaacc ttataacatg
    31261 tgccgcgagt atcctaacga gaacattgtc aatcctacag ttaagttccc aattaaaaca
    31321 tcaaataatc ctatttctgg ttttgatatt tcagaagaat taataatagg tgaaacttat
    31381 acggtatctt taaaagggac taagccagct actaaagaat tccacttata ctacggagaa
    31441 gcaaattacc agcagttgca gcatcaggca actttacttc ctgtggaagg attaacagat
    31501 gtgtggagtg ccacttttac agccagaaat actagtgaaa cgacaaattt acttagagta
    31561 gcgttatggc aaaaacctaa ttctgcaaca tatgatactg ttcaaattga ctggctcaag
    31621 attgaaaaag gcaacacccg aacgcccaat attagtgagt ataaatatcg tggtactggt
    31681 atgcgtgatt caaacaatcc aaaagattat gtttgggatc tagcaccaga atatgtcgaa
    31741 gataatttgg ccacagatat taaaatttct gaaattactg gtaaagcaaa caattatacc
    31801 gatgggaaag tatcggagat taattcgcag ttgactgctt caattaatga agtagacacc
    31861 acagctaagg atgctcgaac aaaagcgaat gctaatgcga ctgctataga tgaattagac
    31921 aataagatcg atgaacgcat taatgatgca gctactaccg cattttatgg cgcacaaggt
    31981 gaagtctcta acaaaacgaa agtagcggag tatggagtag gaacagggta tactactaca
    32041 gctgtaggag aaacacattt cgaacgtcaa tctaacggat acgttaaatg cttaaaagct
    32101 ggtaagtatt caattagtgc gcaaatacgt gtgcaattgg gcgggaaata tgcttcatgg
    32161 ttatatactg atttatataa agatggaact tctgttgata atttagtatc ttatggggtc
    32221 gacgccttgc aaaatagatt tgcagctagc ggaaacattg taactgattt agaagtaaac
    32281 gatgtactaa atatgcgtac tgaaatcggc gttgcagatg gaattttaca atttacatct
    32341 tgccgtactt tagtcttgac tagactatct gattaataca atatttataa ttttgctatc
    32401 aacacgctca aaggagggtg ttttttattt tgcaatgaaa ggaggctagt tggttgaaag
    32461 acgaagcaat acaagacgtg gtagaacgct tagtgcgtat tgaaacgaaa ctggataatt
    32521 acgaattatt acgtgaaaaa gcggaaagtg caaaagatag agcggatcag gtatattcta
    32581 ttgcgcttaa taatgcagaa gacatcaaag aaatgaaagc caataataaa tggtcgtggg
    32641 gttacatgat tggtttaggc attacgatca ttggctattt cttgactaaa ttgtaaagga
    32701 ggtgagaaga aatgatttta ccagataagt attatcaagt cattaaatgg acagttttaa
    32761 cagtattgcc agctgcatct gctttagtag ccatgttagg gaaagcgtat ggatggaatg
    32821 gaacagatat gatagtgctg actatcaatg cagtagcaac atttttaggc gttatcactg
    32881 gtgtgtcggc ttataatttg aaaaaatagg aggaaacaaa tgaaaaagaa aattactatt
    32941 actgcgatga gcctgttaac ggctcttttt ttattgccaa ttaacggatt tgcctatact
    33001 atcaacaatg aatttaattt gggcgcaaat gaaggtagct cacaagtagc aaataatcag
    33061 tacattttac tgcatgaaac ggctaatgaa acagcaacag gacgcaatga agcgcagtat
    33121 atgcaacgtt catggactag tgcttacact gcttacattg tgggtgatgg cggaattgtt
    33181 taccaagttg gacaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgccaac
    33241 agtcctgtgc agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac
    33301 aaggcatacg ttgaattggc tagagattca gcaatgaaat atggtattcc attaacgttg
    33361 gacactcctt ataaccaacc gggaatcaaa tcgcatttat gggtaacaca aaacatctgg
    33421 ggcgatcata cagatcctta tggttatctt tctgaaatgg gcgtaagtaa agaaaaatta
    33481 gcatatgatt tagctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca
    33541 gtcattgatc caactagagc aggtgcagca aatcctacgc tgacagatgg aacaaattac
    33601 gcccacattg atcagtttgg ggaaatcgaa aacgcaaact tgcatgtggc tggatggcac
    33661 attgctaact ataaatacga gtatatcttc attatggact acaatactgg aaaagaacta
    33721 gctagagtaa gagctgatgg gatttataga ccagacgtaa atcaagctta taatacttta
    33781 ggaaatgttg gctatcatgt atctttcaat atgcgtaatt ttcctaataa gaaagtctat
    33841 gtaatgatgc gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt
    33901 catgataaac gctggtactt gaatattcct aaacgataaa aatagcccct c
//
LOCUS       DACDNO010000027        33636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDNO010000027 DACDNO010000000
VERSION     DACDNO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.027"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(105..476)
                     /locus_tag="IWI22_001354"
     CDS             complement(105..476)
                     /locus_tag="IWI22_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9168755.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            691..876
                     /locus_tag="IWI22_001355"
     CDS             691..876
                     /locus_tag="IWI22_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168756.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(959..1147)
                     /locus_tag="IWI22_001356"
     CDS             complement(959..1147)
                     /locus_tag="IWI22_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168757.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(1171..1434)
                     /locus_tag="IWI22_001357"
     CDS             complement(1171..1434)
                     /locus_tag="IWI22_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168758.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            1592..1849
                     /locus_tag="IWI22_001358"
     CDS             1592..1849
                     /locus_tag="IWI22_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9168759.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(1920..2111)
                     /locus_tag="IWI22_001359"
     CDS             complement(1920..2111)
                     /locus_tag="IWI22_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168760.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(2252..2572)
                     /locus_tag="IWI22_001360"
     CDS             complement(2252..2572)
                     /locus_tag="IWI22_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168761.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(2595..3029)
                     /locus_tag="IWI22_001361"
     CDS             complement(2595..3029)
                     /locus_tag="IWI22_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9168762.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            3364..4002
                     /locus_tag="IWI22_001362"
     CDS             3364..4002
                     /locus_tag="IWI22_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9168763.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(4326..4409)
                     /locus_tag="IWI22_001363"
     tRNA            complement(4326..4409)
                     /locus_tag="IWI22_001363"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4373..4375),aa:Leu,seq:cag)
     gene            4937..5437
                     /locus_tag="IWI22_001364"
     CDS             4937..5437
                     /locus_tag="IWI22_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9168764.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(5627..5971)
                     /locus_tag="IWI22_001365"
     CDS             complement(5627..5971)
                     /locus_tag="IWI22_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9168765.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(6125..7045)
                     /locus_tag="IWI22_001366"
     CDS             complement(6125..7045)
                     /locus_tag="IWI22_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9168766.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            7232..7723
                     /locus_tag="IWI22_001367"
     CDS             7232..7723
                     /locus_tag="IWI22_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9168767.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(7747..10122)
                     /locus_tag="IWI22_001368"
     CDS             complement(7747..10122)
                     /locus_tag="IWI22_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9168768.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            10448..11074
                     /locus_tag="IWI22_001369"
     CDS             10448..11074
                     /locus_tag="IWI22_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9168769.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            11097..11801
                     /locus_tag="IWI22_001370"
     CDS             11097..11801
                     /locus_tag="IWI22_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9168770.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            11993..12826
                     /locus_tag="IWI22_001371"
     CDS             11993..12826
                     /locus_tag="IWI22_001371"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9168771.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            13023..14951
                     /locus_tag="IWI22_001372"
     CDS             13023..14951
                     /locus_tag="IWI22_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9168772.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFSESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            15089..15544
                     /locus_tag="IWI22_001373"
     CDS             15089..15544
                     /locus_tag="IWI22_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9168773.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            16014..16283
                     /locus_tag="IWI22_001374"
     CDS             16014..16283
                     /locus_tag="IWI22_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168774.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            16683..16937
                     /locus_tag="IWI22_001375"
     CDS             16683..16937
                     /locus_tag="IWI22_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9168775.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(17026..18072)
                     /gene="holA"
                     /locus_tag="IWI22_001376"
     CDS             complement(17026..18072)
                     /gene="holA"
                     /locus_tag="IWI22_001376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9168776.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(18110..20383)
                     /locus_tag="IWI22_001377"
     CDS             complement(18110..20383)
                     /locus_tag="IWI22_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9168777.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(20376..20915)
                     /locus_tag="IWI22_001378"
     CDS             complement(20376..20915)
                     /locus_tag="IWI22_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9168778.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(21051..21269)
                     /locus_tag="IWI22_001379"
     CDS             complement(21051..21269)
                     /locus_tag="IWI22_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9168779.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(21266..21511)
                     /locus_tag="IWI22_001380"
     CDS             complement(21266..21511)
                     /locus_tag="IWI22_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9168780.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(21627..22355)
                     /locus_tag="IWI22_001381"
     CDS             complement(21627..22355)
                     /locus_tag="IWI22_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9168781.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(22514..23557)
                     /locus_tag="IWI22_001382"
     CDS             complement(22514..23557)
                     /locus_tag="IWI22_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9168782.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(23547..24038)
                     /gene="coaD"
                     /locus_tag="IWI22_001383"
     CDS             complement(23547..24038)
                     /gene="coaD"
                     /locus_tag="IWI22_001383"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9168783.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKNL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(24035..24595)
                     /gene="rsmD"
                     /locus_tag="IWI22_001384"
     CDS             complement(24035..24595)
                     /gene="rsmD"
                     /locus_tag="IWI22_001384"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9168784.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(24714..25052)
                     /locus_tag="IWI22_001385"
     CDS             complement(24714..25052)
                     /locus_tag="IWI22_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9168785.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(25036..25473)
                     /locus_tag="IWI22_001386"
     CDS             complement(25036..25473)
                     /locus_tag="IWI22_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9168786.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(25463..26587)
                     /locus_tag="IWI22_001387"
     CDS             complement(25463..26587)
                     /locus_tag="IWI22_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168787.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(26608..30036)
                     /locus_tag="IWI22_001388"
     CDS             complement(26608..30036)
                     /locus_tag="IWI22_001388"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9168788.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(30062..31225)
                     /locus_tag="IWI22_001389"
     CDS             complement(30062..31225)
                     /locus_tag="IWI22_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9168789.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(31228..31530)
                     /locus_tag="IWI22_001390"
     CDS             complement(31228..31530)
                     /locus_tag="IWI22_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9168790.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(31644..33416)
                     /gene="recQ"
                     /locus_tag="IWI22_001391"
     CDS             complement(31644..33416)
                     /gene="recQ"
                     /locus_tag="IWI22_001391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9168791.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         9866 a         6808 c         5738 g        11224 t
ORIGIN      
        1 ctttttgcag ggggattatt gtattatttt tactacttgt atttagttta cataattaaa
       61 ttatgaataa taaaatcggg cgtattctta atacaaaaac tgatttaccc aataattgct
      121 aatgctttct ccggaacttt ttctctagga acttcctcat acgtttctat gtgtgctcct
      181 ttattgctaa ttttcaaata atgattttct tttaacacag aaagaccatt aaattcaacc
      241 gttcgttctt tcccattggc atcagcagct ttctgcacat accttgctgt accatattca
      301 tttacttcct ctggtttttg tgtctttaca tagacctctc ctttttcaac taatggattt
      361 agctgatcaa gtataccagg gatttctcca taacttcctt cagtataata tttcagtcca
      421 aatagcaaaa taccactaat cgccgccagt ccaatcaaat atttaatcac tttcatcttt
      481 ctacctcctt gttatatatt cagtatatgg gtcagttaaa gaaattccat ccgtcccaag
      541 ttggagattt ttctcctact ttagaagcga gaatcgagtg tttttttagt aaaaatgatc
      601 gttatttgat attattgacg tgtggataaa tcagtttttc tcgttttgtc catgtaaaaa
      661 atcatgttga tagcaaagga tgtgttcaaa atgaatcaag aagaattatt tcaaaaagtc
      721 aaggaaatga ttaagaacgg caatttcgat ggcgcaaaac gatttatcga agaacataaa
      781 gaacaattag gaccttataa agagaaagcc caaaatcttt taaaagatgt aaatatcgac
      841 agtgtcaaaa acaaatttaa aaatctattc aaataagtaa aactttcact tttgtaaaat
      901 caaaggagga caatgctgct gcatcgtcct cctttggttc ttttctgaat acttcatact
      961 aaaataaacg tgtcggatcg taatgataag tcacattgcc gttcagtgag gcttttccca
     1021 ccacatattt acgattctcc aacttcagcc acgcttctcg aaaagccatt aattcttctc
     1081 tttttacatc aatcttttcg atttttccat cgatcaaatc ttgaataagt tgttcatatg
     1141 gattcatttt tttcctccat ctgctgtcaa tcaataagtt gtaaaaatag gttcatatgc
     1201 ttttttggta tctagatctt ctctgacttt gactgtcgta taaataattt tttctactgg
     1261 gatactataa taacttcctt caccattatt gaatcggtga acgtctttct ttgctaaaat
     1321 accatttatt ttattcgtca gtacacgttg ttcgacactg ttacttacaa catagtaaat
     1381 cacccgaata ttattgatca aatgcaaatc gatttctaac actacatgat ccatgaaaat
     1441 cccactcctt ctttcacttt gattatagca agtttctcct ctaatttcaa aatcgataca
     1501 gattttaccg gataaatcca caaaactgct tgcgccattt cgcacaaagt gttattttgt
     1561 atcatggact ttatttatcg gaggcaatag tatgatcgcg atcggtcaat ttgtttttta
     1621 tattcctttt tttattatga taagcattct tttctattac ataaaatgga caaaaaagaa
     1681 gttttcagtt ttactggcct ctttacctgc tgtatatttt acttatcaaa ttttttcttt
     1741 taggcattgg gaaacaacgt ctgtattaat aacacacatc atcgaattga cgctttcagt
     1801 aatttttctt attatttgga tctatttttt atataagaat cagaattgaa tccctcgcat
     1861 gtttaaaaag aaaacgatta aagatgcagc aaatttctgc cgatctttaa tcgttttttc
     1921 tattctgtta taccaaaaaa atgtttgaat cggtgaagga tcgttccttg ttttaattct
     1981 ataaattctt gtgcgatcgg gtacttttct tccaaatttt ctttgttctt ttcgatcagg
     2041 cttttcgctt cttcatattt tttatctgca catagtgctt ttacttcctg taatactttt
     2101 cctgcttcca tttgcctcac cctcttgcca ttttattgct ttaagtttta tttaagttat
     2161 acgcattatt ctagtttcat cccaaataac cttttttcat ttttactcct cgaactaatc
     2221 agttttctaa aggctgattg gttcgtcttt tttatttttc ctccatttca ttctgctctt
     2281 ctgttaattt atctaatgct ttgattgatg ctgcggctct tgcataatca tagctgtata
     2341 attccataaa tttatcaggc agaacaaacc acgctggatg ataaccgtta gctgtgacag
     2401 ggaatccttt atcttgttgg ttatctccat cccatctcag agcaacacct ttttttcctt
     2461 tccactttac taaagcgatc gagtatccct tctttgtttg tgtgccgtct tcaataattg
     2521 ctaaaatttc tattttttct tttggcgcac ggacttcttg aggacgaacc attttctatt
     2581 cctcccttat ctcattactt cacgaccaac acactgcaag gggcatgtga tacgacataa
     2641 gaagcggtcg atccaactaa tgcttgttgt atagcacctt ttcctgtcgc tcccataacg
     2701 atcaaatcaa tattttcttg tccagggatg acattggcaa tcaaatttct tggatctcca
     2761 gtttcaacta ttgcgtggat ctcagcaatg ccgtactctt tggcatcatt gatttttttc
     2821 agcatggcta cttctgttct ttgtttttca tctgcataga tttttgaaaa ggcataggca
     2881 ctatgggtca gctcaacttt gttgatgatt gacacgataa ataagtgggc ttcattgtcc
     2941 tttgctaagt caagggcttc agcgaacgct ttgatcgatt gtcttgatcc gtcaactgct
     3001 accatgatgt tctggtatct tttactcatt ggaaccgcct cctttttctt ttattgtaac
     3061 atctcaagca aaaatacaaa aaatatacgc aatatgtaaa taaaatgatg aacaaaagca
     3121 atttctttca agttatttat acatttcttt tctcatcaga acacctgttc taatcccatt
     3181 atttctgcat tatgtgaaat tcctcatcat ttttcacatc gagcgaacat cattctgtta
     3241 caaaaaacgt ggatttgtca taaattgtca tgcttctttt acctatgaaa tattttcacg
     3301 tgttaaagta ttcctcgttg aacacaacaa ataaaacttt ttaacgaata aaggagattt
     3361 ttcatgacat cacttaaaac tttacttttt ggaacaactt tggctgccgg cgctgcattc
     3421 ttcatgggaa cgactgctca tgcggacgaa gcttataccg ttcaatcagg tgatactcta
     3481 tcaacgattt ctcaaaaata cgttggtgat aactccttga tcaatgccat tgcagaatca
     3541 aattcaattt ctgatattaa cctgatctac tcaggacaac aattaacaat cccaacagaa
     3601 ggctctgcac aagcagctgc tgaacctcaa gcagccgttc aagaagcacc agtccaagct
     3661 gaacctgttc aagctgaaca acctgttgta caagaaacgg ttcaaacgga aacacaagca
     3721 gcgcctgttg ctgaaacaca acctgctcct gctgtaacag aaactgctgc tactccagca
     3781 agtacaagct ctgcaaaaga atggatcgca caaaaagaat caagcggttc ttacacagca
     3841 acgaacggac gttacatcgg ccgttaccaa ttagattctt cttacttaaa tggtgactac
     3901 tctgctgcaa accaagaaag agtagcagaa caatatgtta cttcacgtta tggttcttgg
     3961 gaagcggcga aagcattctg ggaagcaaac ggttggtact aagattcatt atttgaacgt
     4021 ttaaacacat tcaaaatata gtaagaggtc atgcgattta ttctttcgca tgacctcttt
     4081 ttgagtttaa gtttagtctt tattccttaa ctatcgtttg ccagtatttc ttgtatcaga
     4141 atctataaat ctactatttg aatcaatcta ttaggtttta aactgtccca ccttcacaca
     4201 aatttttttt gatttatttg aatctggttc cgttgtttaa aatttttcct atattctttt
     4261 agataatatg ttattttctt tctcatcgct ccaggtgtac cttctcttta taaaaaaaca
     4321 aattatgcgg ctaagaagac tcgaacttcc acgaggtcac cctcactagc gcctgaagct
     4381 agcgcgtctg ccaattccgc catagccgca tcacatcttt tagtttagga ttacatgtgt
     4441 tttttgtcaa gaacatattt ttgttctttt tcatttctgt acgccctccc ttatgccatc
     4501 tacacaaata attcatccga gcaactccat catctattga gggttggtga aaaaatctgt
     4561 tttatccata tgatcgattt caatgttaaa cgaacgactc tgggattagt aactatttta
     4621 tttttttctt catttctttt ttcctctttt tctcgattca gctatcttct ttcatttgga
     4681 tattgattat ttcttctgtt gattactatc cttctggaat tctagacgct gaagtaattc
     4741 acgttttctt tcttctagag atcataagta acgaatgaac gaacttcaaa taattttttt
     4801 attgtaattc atatttgtat tgttcgatga ttcttaatac gatatgacac aataaataaa
     4861 aataggcatg gctatttaca acaatagtgc tatagtagaa aacaaatgaa taaggttact
     4921 taaagaggtg aatacgatga aactgatcaa actatgggaa gcagatttaa agaaagctta
     4981 tcagctacaa actagctttg gaccaaatga gagtggattt atcaacaatg cttatggcta
     5041 tacatttgct gaatttttag attatgtaaa aaaatgcgaa aaaacgtctt taggaatcga
     5101 tcttcctgag agttacgttc ccgcaaccgt ctttatttta gaaagtgatg aacatgagta
     5161 tgtaggaatt tttaatttgc gccataaact gaacgaaaca ttaagaaacg gcgctggcca
     5221 tattggttac ggcatctccc ctaattatcg taaaaaagga tatgccacac aaggattagc
     5281 attagtacta caagaggcaa aaaaaatcgg cattgatgag gcacttttgt ctgtaaataa
     5341 aaaaaatctt ggcagtctta aagcacaaca aaaaaatggg gctgcgattt atcgtgaaga
     5401 tgagaaagaa tactatacga aaatcaaact gatgtaaaaa agaaggattc caaaaggaaa
     5461 gggatttgaa tgtcctttat caatagaaca catttattta attaacttat aatatagcag
     5521 acaaaatttt ataaacaaag aaacctttta tctcttttct tctagaaagg ataaaaggct
     5581 tttttgtttt tcctatttgc ttctcatagc tagtcagcca agatttctat cgtatcccca
     5641 acgcaatccg tgcataacgg ctcatcttat ctgtcgtcca tgctggatac cagactagtt
     5701 tgacttctgc tttcgttact tctggcactt cttttaatgc gtcatggata gattctgtta
     5761 aaatatctgc taatggacag cccatagttg ttagagtcat cttaatgact gtttctccat
     5821 tttcaggatt gaattctata tcataaatca agcctaaatt gacgatgtcg attcctaatt
     5881 caggatcaat caccatttcc aaggcagcaa gaatacgttc tttgattagt tctatttctt
     5941 gtccagaccg ttcttggttt gcttcgctca tcgtacgaca ctccttaaca cgatttttat
     6001 ttatcatact ctttttttag ctgcttgtaa atgagaattt agaagtaaga ttctcgttta
     6061 aatttataaa aatgttctta ttagatgtaa tagattaaat ttattctaaa ataaacctga
     6121 ttcctcattt tttttgtgaa cgatttttta tttctggcaa tacgccagtt gttcgataca
     6181 ttttttgttc gttttcattt gcaaaccggc caaatactga atgagttggt aaaatattga
     6241 taaatgcatc gtcgtcgatt tcatgaacga tctgatcaag atcatacatt tcgtagcggg
     6301 tgatgaccat catgatcatg ttcccttgtt tttttgaata gccaccgaca gacggcagta
     6361 atgtcattcc acgtaccatc cgttttgcta gttcttctgt tacctcttct ggcttgacgg
     6421 taacgatcat tgctgttact ttttgatgac tcgtgtggat ggcatcaatt acttgagtca
     6481 ttgcataaat ggaaataatc gtatacaagg cactttccca gttaaagaag aagcctgcga
     6541 tgactacaat gataccattt agaatcagca tgtagtttcc gactgttttt ccagttgttt
     6601 gtgaaagtac aagagagatg atatccatcc caccagttgt aaagcccatt tttaaagata
     6661 atcctacgcc aagacctaat aaaacaccgc ctactagggc attcatcaaa atattatcag
     6721 tgatctgtcc gactggaagc agcatcgtca tcaccgatac acatacaaca ttcacaaaac
     6781 taaggatcgt tgctcgtttt cctactttga gaaaccctaa tacaaacaca gggatattca
     6841 aaagaaaaat aaatgttcct gtgtctatct tgatatgaaa ccattcatat aaaagcgagg
     6901 aaatgatttg agcgatccca ttcatccctg cagaaaagac attggctgga atcaaaaaat
     6961 aatttaaagc gaccgcagcg gtcaatccgg ttatcaaaat aaccactatt ttctttaatc
     7021 tttcattttt atgatacgca gtcataattt tactcacaag cacctctccc ctactttggt
     7081 attccaaatg taagaataac agtttatcta ttaaaataaa atacttcgaa ctcaaatgag
     7141 aagaatttta gcaaacaggt aagaatctgc cctaatccta tgataagata gatgtatggt
     7201 aaatatttat atgtatatca ggagggttcc tatggcaaaa gatgctagct tcgacattgt
     7261 atcagaagtc aatacagaag aagtaaaaaa tgcgattcag caaacaacaa aagaattgtc
     7321 caatcgcttt gacttcaagg attctacagt agaaatcaaa ttagagaaca gcaatctagt
     7381 tgtattgagc gatgatgatt tcaaaatcga acagatcaaa gatgtattat ttggtaagct
     7441 gatcaaaaga aatgtgccaa tcaaaaatat ccatttttcc gaatcagaac acgctctagg
     7501 tggcaaagct cgacaaactg ccgatttggt caacggaatc gatcgtgaaa acgcgaagaa
     7561 aatcactaca gctatcaaaa atgaaaaact gaaagtaaaa gcacaaatcc aagaagatca
     7621 gattagagtg acaggtaaga accgtgatga tctacaagct gtgattgctt tattacgtaa
     7681 acttgactta ccaatcgagt tgcaattcac taactatcgt taatcaaaaa acgaggctga
     7741 cgtctgtcat tcctcgtttt tttgatttac tgcttctttt gataaccaaa aacgctgtat
     7801 tccttcttcc aattccccaa cacttcgtac ttctcttgct gtttgccaat gttgtgggaa
     7861 taaacgtcga taagaaaaac tagaaaatcg ttgatctgcc aatacaacga ttccttgatc
     7921 cgccatgcca cgaatcactc gaccaccagc ttgcaagact ttgttcattc caggaagctg
     7981 ataagcgtaa gcaaatccaa ggtgttcctt ctcatcataa tatgatttga tcagttcctg
     8041 ctcatgattc atctgcggca aacctacacc tacgatcata ctgccgatca atcggtcccc
     8101 tctcaaatcg attccttctg aaaaaatccc gccaagtacg caaaatccaa tcaatgtttc
     8161 agcgggattt gtttgaaatt ttgttaaaaa tcgttcgcgt tcttcctcat tcatgtccgt
     8221 ttcttggatt agcacattgc tgtcgggata ttgctgtctg aatgcttcca ccgcaagatc
     8281 taaatattga taagaaggaa agaaaacgaa ataattcccg acctttccat cagcaaaagc
     8341 atgaatcgtc tctactaatt ttgccagact gtgttccctt tttcgataag tcgtctctag
     8401 gtaatttgcg atgaaaactt gctggtttcc ttctgggaaa ggactcggca gtttgtaagc
     8461 cagacttttt tgtcccccca gtgtttcctg ataataagag agtggtgaaa aactagcaga
     8521 aaaaagaata ctgctttttc ctttatccaa cgactgctca agaaacaagc ttggatcaat
     8581 acaaaactct tttactatca gatcgcgata cgttttttcg actgttgttt cgtagtgatc
     8641 atcataaaat tcactgacct tcaaaaaatg aagcaaatca aaataataag gaagcaattg
     8701 ttcttgtgat gggtgctcag gaaattctgc cagccattct ttaatttttt cgcttaattg
     8761 ataacctgcc tttactaaag attcagccgg tgctttttga tgatgataat cccaatgatc
     8821 ttcttttgct atttctcgga tcgaatcaaa ttcttttaat agtttgttga aacggcgatg
     8881 aagttttttg aattcctttg gtatagcttc cttacttcgt ttcgtttttt cataagatag
     8941 ttctgccgaa tacatctctc gggaacgatt caccaaatta tgtgcttcgt caatcaaaaa
     9001 cagatagtcc tcgttttttt catcttcaaa aaaacgtctt aaataaacag ttggatcaaa
     9061 taaataattg taatccccaa tgattacgtc acaccagata ctgacatcta atgatagttc
     9121 gaaaggacaa agtgtatgct ttcttgcata agtttcgatg acttcacgag tgatctgatt
     9181 ctcatgattc aaaagatccc ataaaccttc attaatccga ttgtaataac cattggcata
     9241 tgggcactga tctggattac aggtcgtctc atctaaaaag cagatcttgt ctttcgccgt
     9301 caaagtgaca ctttttactt cagctcctgt atcttttaat gctgtcatag catcttcagc
     9361 tacttgtcta gttattgttt tagccgttag ataaaagatc ttttctccct cttcttcccc
     9421 tacagctttc aaagcgggga ataacgtaga gattgttttt cctgttccgg taggtgcttc
     9481 tacaaacaat ttctgtttcg tacggatcgt tttataagca gctaccgcta gttcacgctg
     9541 acctttccta tactcatcat gggggaattt caaagccatc aaagaggcat tgcgtacagt
     9601 gcgccagttt tcttgaaaaa caagccattc tttataatct tcagtcagct ttttaaaaaa
     9661 agtttctaac tgctctcttg tctgatgttc aacctttctc gtgatcactt cttctgccgt
     9721 ctggaagtaa gtcaattgca gatttatttc ttgtaaatca tgttcttgac tatagatata
     9781 ggcatacacc cttgcttgat gaaagaaaag ttccacttgt tctggttcta aatcttcaaa
     9841 tcgtggttcg gaggttttga tttcatctat ataataaata ccgtctcttt tgaaaatacc
     9901 gtctgcacgg ccttcaacga tcaggtaata ttcatctaat tgaactgttg tttttaaaaa
     9961 gacttctttt tcatagtttt ctcccgcatc tttttggagc ttgcggtgga ttctcgctcc
    10021 ttctaatgca gtatggttac tttttttacg attgtcgata cttccacgtc gcaaaataaa
    10081 ttctactaag tgccgcacag atatttttga ctgtttgatc atttccacca cctctttctc
    10141 tctaagatta cctgaaaaaa cctagtttag caaaaagagc tctctttcca aacataaaaa
    10201 aacaccctgc tgattaggca gggatatagg atatagaatt tggatttttg gatgaatcta
    10261 atgtaacaag gacatattaa aatgctatga aaacttactt tccttctctt tagaatatcc
    10321 aaatactttt catatagtct gataattcag ataagtaaag agaaataagt acctgttttt
    10381 gtttcctagc aatgaatgcg ttatactaat ggatgaaaga agttttggct atcaaggagg
    10441 aatgcacatg catatcgaaa gattaaaaac aggaatgatt gaagaaaact gttatttggt
    10501 ctacaacgaa gaagcacttt tgatcattga ccctggagaa gatgcggaac gaatcaaaca
    10561 agaaatcaaa aaaacaaacc aacagccggt agcgattttg cttacccata ctcactacga
    10621 tcatatcggt gcagtggaac ctttacgcgc gtattatcaa atcccagttt atgtcagccc
    10681 actagagcaa aaatggctag gtgatccgat tttgaatctt tctggactcg gtagacatga
    10741 tgatattgct gatgttattg tctcgcctgc agaatatgaa tttgaaatga aaacttatac
    10801 tttaggaaac atgtcttttc gcgtcgtccc taccccaggg cattctatag gaagcgtcag
    10861 ctttattttt gatgattttg tcgtttctgg cgacgccctg tttagaggaa gtatcggtag
    10921 aaccgatctg tatacaggga atttagaaca attacttcat agtattcgga cccagctgtt
    10981 tgtattacct gatgaatttg cagtctatcc aggacatggc gatgcaacga cgattgaaca
    11041 ggaaaaaagg acaaatcctt ttttcaatcg ttaaaacgac ttaggaggag aataaaatga
    11101 cagaaactac actttatatc gtacgacacg gaaaaacgat gttcaataca attgaacgag
    11161 tacaaggctg gtgtgataca cccctcacaa agcaaggaca agaaggcatt cattatttag
    11221 gtcgcgggtt aaaaaatgtc gacttttcat ttgcttattc tagtgacagt ggtcgtgcaa
    11281 tcgaaactgc ccgtattatt ctaggagaac attctaaggg aaaagaaatt ccttatttta
    11341 ttgaaaaaag gattagagaa tggtgtttcg ggtctcttga aggtggatat gacatggaaa
    11401 tgtggggtgt gatccctcgt gtattgaatt ttaaaacata tgatgaaatg tttaccacag
    11461 aagtttcttt tgaacaaatt gctaatgctg tatacaactt agataccgct gggtgggcgg
    11521 aaacttatga aacattgcga gaccgtgtat ggacaggatt tgaagatatc gcccatcact
    11581 gcgaaaaaaa tggtggtgga aatgttttag tcgtatctca tggtttgacg atcgcctttt
    11641 tgcttaatct gatcgatcct aaacagcctg ttcgtgctgg gcttttaaat ggcagcgtaa
    11701 caaaggtcac ttatgcaaac ggtcgatttt ctatccacgg catcaatgat acgtcttata
    11761 tcgataacgg gaaaacacat agtcgcgact cgtttctata atggggatat tttaattttt
    11821 accagagaat cataaaaggc aaagtctgaa cgagggcttt gtcttctttt ttctcttcgt
    11881 gtacttatct gctttatttc tcaagttttt cttaatatta aaaaacagat tgggtttctc
    11941 aaaaaataac ggtatactat aagatgtatc ttaattggga aggaaggaat ttatgttttt
    12001 tgcaaatatt atcaaagcag tgattctcgg tattattgaa ggaataacgg aatggctgcc
    12061 catcagtagt actggtcatt tgatcctagc agatgaattc atcaaactgg atgccagttc
    12121 acaatttatg tcgatgttca atgtcgttat ccaattaggg gcgatcttgg ctgtcgtcgt
    12181 tctttatttt cacaagttaa acccattttc tccaagtaaa tcaacggtag aaaaaaaaga
    12241 tacgtggaca ctttggttta aagtgatcgt tgcttgcata ccagccatca tcattggttt
    12301 gcctttggat gattggttag aagcaaattt ccacaagttc ttaccagttg ccattgtttt
    12361 gatcgtttac ggtattgcct ttattattgt cgaaaaacgc aacaaaaaca aaacacctcg
    12421 ttggacttct ctgaatgact ttacgtacca agcagctctt cttgttgggt tattccaagt
    12481 cttggcactt gttcccggaa catcccgttc tggcgcaacg atcctaggat ctatcttgat
    12541 tggtgcttcg cgttttgtcg ctacagagtt ttcatttttc ttagggattc ctgtcatgtt
    12601 tggcgcaagc ggcttgaaga tcgtcaaata tttagctgaa gggaattcat tcaaaatgga
    12661 agaaactgtt gttcttcttg taggaacatt cgtatctttt gtggtttccg tcttagctat
    12721 caaattctta ctaagctatt tgaagaaaaa cgactttacc gcttttggct ggtatcgtat
    12781 tatccttggt atcattttga ttggttattg gtttattgca atgtagaaaa aaccagaaca
    12841 cttctcttat ttgagaaatg ttctggtttt ttattttctt taataataat cattataaat
    12901 aataaaaaca acatcaagct taatcaccaa atgataacca ttatcgttct catttagctg
    12961 ttattattat caattacatt tttattatgc tatccttgtt ttgacaagga ggtctaataa
    13021 cgatgactaa tcagaaaaaa gcttttgcag caacaatcgg ctgcctttta tttatgatct
    13081 tcggttttat cttcgaccga ttctctctta ccatcacccc acttttttat atcagtgcca
    13141 ttatcatcgg tggatataaa caaacatcgg aaggcttgaa agaattgtgg aatgatcgga
    13201 cactgaatgt ggatttattg atggctttag ctgctattgg tgcctgtttg atcggtaatt
    13261 actttgaagg agcaatgttg acattcatct tttgtttaag cggtgcatta gaggaataca
    13321 caactaataa aagtcaaaaa gaaattactg ctttgatgaa tcttcaacca caaaaagccc
    13381 aattattaaa agaaaatgga caaatgcaag aagtagatgt ttctcaacta aaggtggata
    13441 acttggtatt tgtcgctaaa ggagcagctg ttccgataga tggcttttca gaaagcacac
    13501 aggcaacgat cgatgagtct gcattaagcg gtgaatctgt gcctgtagaa aaatatttag
    13561 gcgatgagat gtatgcagga acgctgaatc aaggaaatcc catcacgatt cgagtaacaa
    13621 aaacgagttc cgaaactgtc tttgctaaga ttatccaatt agtagaagaa gcgcagaata
    13681 cgccaactca aacagcttca tttatcgaac gaatcgaaaa taattatgta aaattgattt
    13741 tgcttgctgt tcctctaatg atccttctcc cccacttttt cctaggttgg agttgggatg
    13801 aaagctttta tcgaggaatg gtattattag ttgtggcttc tccttgtgcg cttgtcgcat
    13861 ctgctacacc agcttctcta gctgctcttt caaattctgc aaaaaatggg attttaatca
    13921 aaggcggtat ccatttagaa aagctttctg aattaaaagc gattgccttt gataaaacag
    13981 gaacgctcac taaaggaaaa ccagttgtaa ctgacagctt tttctttgat gaaaaggatt
    14041 tggcacaaca acttcttgtt gctatggaac aaaaaacgac gcatccactt gctcatgcga
    14101 ttttgcaata tttcgattgc acaatacctg aagaaatcca atacttacca atagaagaaa
    14161 ttactggttt tggattacaa actacctatt taggtgcgca atggaaagtt ggcaagcacg
    14221 catatgatcc tgagacgatg ataatcagca aagagattgc ggaaatgatc gaacgtttgg
    14281 aaaatcaagg caaaaccgtg atttatcttt ctaaagatca gcagttaatt gctgttttag
    14341 gtctgctaga tattccaaaa gcaaataccc aacaagtaat ttcctacttt aaatcccaaa
    14401 acatccatac aagtatgata accggcgacc attctggcac agcaaaggcc attgctgaac
    14461 aagtagggat tgaacattac tatgcaagct gtactccgga agaaaaaaca caactagtca
    14521 aaaaagagaa tgaactttac caagtaaatg cgatggttgg tgatggagtc aatgatgcgc
    14581 ctgctttagc agccgcttct ttaggtgtcg ctatggggca agggacagat gccgctatgg
    14641 atatcgcaga catcgtctta atgaaaagcg acttaaataa atttgtctac agtcatcggc
    14701 tatctttaaa aatgcggcgg atcatcaaac aaaatattat tttttcgatt cttgttattc
    14761 tcttgttgat tgcttctaac ttcctgcaat ttttaaatct acctcttgga gttgtaggac
    14821 acgaaggaag cacaatattg gttattctga atggcttgcg tttactaaga cctttacctg
    14881 atctttctga ttcttcaaaa aaatgtaagt cttgtccact atacaaagcg cggctgtctt
    14941 ctgtcaaata agcaaagtgt ttttttatga ttttgtttat tttatgaaaa aaacagctta
    15001 tgaataggtc ttgaaggatg aattcctact tttgtaactt tctaaaaaca tgttatgatt
    15061 gtttcgaaga aagtgaggaa tatatttcat ggaaagttgg ctttttttag gaattatttt
    15121 acttgtagca attttagcta aaaatcaaag tctgatgata gcggcagctt ttgttttgat
    15181 tatgaagctc ataccagcct cccgttcctt tttcccttgg cttcaaggca aaggaatcaa
    15241 tattggggtg acgatcatca cagcggctat tctaatccct atcgcaacag gagaaatcgg
    15301 tctacgtgat ctattagaag cctttaaatc ccctattggc tgggtagctg ttttttgcgg
    15361 tgggctagtg gctgtcttat cttccaaggg tgtaggattg attgcttctg atccacaaat
    15421 cactgttgca cttgttttcg gcacgatttt aggtgttgtc ttcttaagag gaatcgccgc
    15481 cggaccggtc attgcttctg gtatcactta ctgtatctta caagtatttg ctttaggaaa
    15541 atagaacaaa ttttgtctat tttaggagat tcttaacacc cccaatttac tgatccgtga
    15601 tatactgaca acgaggtgat acaatgttcg aaaaattaaa gtatttcata ggaacaatcg
    15661 gctggtttct tctagcggta cttttgattc tgggcttagc ttatctatta tttcctgatt
    15721 tgatacaaaa tgtgtttgat actgcttttt gattgaccta ttaaaacgat ataggggatg
    15781 ctcctgcgat gcaggggctt tttttaatca atgttttgta ataatttagg ccgtgccaat
    15841 gagacttcct atagaattcg atgctctttt ttctttttgg ctcttctgct cttttttgtt
    15901 atactagatt acagtggaca aaagaaagaa tgatcgcatc cacttcaacg aatatcaaaa
    15961 tggaagaatt agtgcctgtt ttcaggttac tttgaaaaga aaggaagacg cttgtggaaa
    16021 cttttgaagg tcttttaatt gatacgtacg ttttacaaga acagccaatg atgctgactg
    16081 ggatcaactt gcagcttgag gaccgtgtga tcaatggcct gcttaaagat cgctatttgg
    16141 caaaatatat cttacgtttg cccctaaata aatatgtgct ggaagtaagt ggcaaatgga
    16201 accgtcggaa ccagttggtc ataaaaaaaa tgttgataaa aaatatcgat gaatacatta
    16261 aaataatcgg tacaccagaa tagcctttac tgatcaaagg atactggtta taaaataact
    16321 gcagtttctt aatagaaaaa caaagaacaa cagggaatcg tatccctttt caaaaaaaac
    16381 tttgcaaacg gttctctttg ttatataata acttagtacc aaacattccc attcaaactt
    16441 tgtttaaata gataaataaa aatctagttt ggtttgacgc tattgcctcc cacaatagcg
    16501 tctttttgat ttttttcagc ttgtactatc cgtttttact gaaaaaaaat cctaataaaa
    16561 gactgttaag gaaaaaaact tgtcatgttc atattgcaaa gcacgctgcc tttaggtata
    16621 ataatgaatg ttgaattatg cggtaacacg cgtaagaact taagttatcg gaggtgaaac
    16681 taatgccaaa cattgaatct gcaatcaaac gtgttcgtac aagcgaaaac gctaatgtga
    16741 aaaattcatc tcaaacaagc gctatgcgta ctgcaatcaa aaaatttgaa gatgctgtag
    16801 cttcaggtgc tgacaacgta gatgcgttat ataaagaagc tgttaaagct attgatatgg
    16861 ctgaatcaaa aggtctaatc cacaaaaaca aagctaaccg cgacaaatct cgtttaagca
    16921 aaaaaatcgc taaataaaag aatcggcttc ggccgatttt tttatttgtc aaaaaaagga
    16981 caaaaacaaa cgaccttccc gcatgctttg ccctcgtctt tcttcttatc gatttcttaa
    17041 caactgccct gtgcgcaaaa cgaatagttg aaaacttaat tctttttcga tttgtcccgt
    17101 tttgatttga taatccatct caactaattg atcaaacaac tgtaccagca aatgttcatc
    17161 gaattttttt acttcctgca tggcaagttt tacacgatat ggatggattt tcaatgtttc
    17221 agcaatattt gcctgctgat aaccaatctt tacaagaaat tttgtctgta aaaataaacg
    17281 taattgagaa agcaaaatcg cattaatttt gattgtttct tccccttgca taactaaatc
    17341 ttcgtataaa cgcaaagctt gttccgtctt cccttttaaa atatattggg tcatgtcaaa
    17401 aatattatgt tctaacgttt taggaactaa ttcttctact tctcttaccg tgatttgctg
    17461 ttgattttgt ccataaagga ccaatttctg taactctctc atcattttgg ataaatctaa
    17521 gtcggtcaac ctcaagaata aatcaattgc acttcgttcc attttgattg aggaattctc
    17581 taaggtattc atcagatatt gacgtacttc tttttcattc aactgttgga cattgatgat
    17641 aattgctgtt tttttcaatt gtttcgttac ttttttccgt tcatccaact tttcatatgg
    17701 cgcaaagaat actaatacag tcgattctaa tggttctttc aaataagccg tcaataaatc
    17761 agtattttgt tcgattccat tgtttacttt ttcacctgtt aaaaaataag gattctctac
    17821 gaatactaac cgttgatctc cgaaaaacgg caacgtctct gcttcagcta ctacctcatc
    17881 taaacttgca ttttccatgt caaaacttaa aaaatttaag tcatcttcgc cctctaattg
    17941 gatttttttg acaatttctg tccgaacttg atcttggaga aacttttctg ttcctaaaat
    18001 caagtaacaa gatgcaagtt tttctttacg tattttttgt aactctgctt gtatactcaa
    18061 attactcacc actttcaaaa attatcctac catgagcaga tgagaattac tagtctatca
    18121 ttgtttccac tagcccatga ggatgaaaaa ctggatgcca gcgataggtg atcattcctt
    18181 gttgatccgt tcgccatgtt tttatctgat tttcttctag tgtctccaat acttcgctgt
    18241 gcgggtgacc ataacgatta tttacgccgc aggatataat cccatgtttt gcttcaatat
    18301 gggaaagaaa atctgtgtta cttgacgtac ggctaccgtg atgacctaat ttgactacat
    18361 cggctttcag attaggataa atcaaaagca attgtttttc cattgcttga ttcaaatctc
    18421 ctaaaaataa aaagcgccta ttgttatatt ccatccatag accaatcgaa tcttcatttc
    18481 ttccctctcc cttttcaaaa ggagtcaata cataaagatc ataataaccg ctgatcctgt
    18541 ccccatgttt gatttcaatg atctcagtac catttttttg agcttcagat aaatgccttt
    18601 gtaatggctc atttcgtcca gcgccccaac cgatataaat ttttttgata gtaaaatgct
    18661 gagataacgt gggtaattct cccacatgat ccgtatcgtc gtgtgtcaag atgagtttat
    18721 cgattacact tatcccctga ccttttagat atggaacgat atttctttca gcaggaggac
    18781 gtatcatacc ttgcgcccac ttttctcttt caaagtgtaa ctgacctcca gtatcgatca
    18841 aaatcgtttc ttttcgaaaa ggactttgca aaaagatact gtctccttga ccgacatcga
    18901 taaaagcgat cgttgaagaa aagcgtaaaa atggcaaagt caaaaaaata aaagaaaaaa
    18961 gaccaacata aaacaagtgt ttcacaggtt gttctttcaa cctatccata caaatcagta
    19021 atccgaaaat atacatccca gatagcacaa ggggcaaacg tcctataatg agagacgacc
    19081 ctgaagaaaa atcgattatt cgctcaaata agattaatcc ctcttctatc agcaaaagta
    19141 aagtttccgg cagccagcga ttgaagagtg tgagaaataa cacaccagga agaaaaagaa
    19201 acttgaatac cggtgcaatc agtaatgtaa aaagactacc taaaataggc cactcataaa
    19261 aatgccatac aatcaacggc atcgtgatca tagtaaacag gacgtgagag gccgtttttt
    19321 tcaaccaatc tccatcaaaa gaaatcagga taaacaaaaa actcatgaaa aaactaagct
    19381 gtccacccgc atgaaaaaga aatcgaggat caaatagtaa aagaagccaa aagacaactg
    19441 caaataagtc catggtggaa taatgcttat tcagcaattt gactagcaat cgcaataaaa
    19501 acagcaaact agcacgcaaa acagaaatag aacttcctgc aatagaaaca taacagatag
    19561 ttagtagact caacggaata atactgacgt gtagtggcca tccgatacgt cgaaataaat
    19621 aatacagcca ccctaaataa aattggatgt gtagacccga tagactgaac agatgcagca
    19681 aaccactctc cctaaaaatt tcttctggtt ctctgaatcc tgcatcttta tagccccata
    19741 ataaagaatt aatatataca cttgtttttt gcggaaattc agattggaca aaggagataa
    19801 aagatcctct ttttctctga agccagtttc gccagtgatt ttgcatggtg atctgttcaa
    19861 tcttttgaat ttggaaccgt ccagaaattc tatggttcct gaagtaagct tcttggtcaa
    19921 aattataaag attccgcttt tttgctggag agatgaactc gccttcagca gtcaattcta
    19981 ttccctttcc tcggaagtta agccatgttt tcaattctgc ttctgtgttt acttgataag
    20041 aaagctggag cttttgttta ttctctgata accttccttc cattgtcaaa aaattaccat
    20101 ttgcacgaat cgtatctgta aatacagtaa tttgcgcaga tgttgttttt tcctctaaaa
    20161 atgaatactc ttgtagccta taagaaatgg ataccgttat gctacacaaa atacacaagt
    20221 acgcgataaa acgtccttct tttagcgaaa accataaaaa aattccggca agaagcaaac
    20281 gataacccca attgtcagtg taatacaaca aacagataat catcgacgta attgccggaa
    20341 aaatcaaccg atttcgaaca tgcctttcta aaacgctaga catgttttcc tgttaattca
    20401 ttttgaagtt ttgaaaaatg ctgtgagtct aaagcaactt ggtgtacaga tacacctaat
    20461 tgttcgatca acgcttgggc ataaggatca tttcgatagt catggagata attgatccct
    20521 ttcacaccag cttgcaacaa agcctttgta caggccaaac atggaaaatg tgtgacatag
    20581 atttctgaac cttccgtcgg gataccaagt tttgcacact gtaggagtgc attcatctct
    20641 gcatggatcg ttcgcaagca gtgtccatcc acgacgtaac acccttcatc tatgcagtgg
    20701 acatctccac tgactgctcc attatagccg ccagcaatga tccttttttc tcgaacgagt
    20761 gtcgctccta cttctaaacg tgtacacgta ctacgtaaag ataacagtac tgcctgagcc
    20821 ataaagtact ggtcccaagg aatgcgttca ttatgtgact tatcagtaac tttttcttta
    20881 ttcgtatcgc atgcttttga agatttttct aacatgtttt atattcctcc gttttttcaa
    20941 gttgattaaa aagtactcat tgcttaaaca aaatattctt taaatataac gaatcatcct
    21001 gtctgacaaa agatcctacc cttcactgcc gaaatgaagt ccgtcatcca ttagcccatt
    21061 ataaaaattc ttcgaatcca tttgttcaag aaattccgta taaaaatcga tacttcacaa
    21121 tcatggcgtt ttgctacttc tgtgagaaca gagatatatt ctttgatcaa cttgttattc
    21181 cgatattttt gttttgtctc atcgactgct ggtggcgtga taaagaaagc atgatcagaa
    21241 ctatagtctt ttttcagctg aactatcatc cactgcagat tttttggtac gtttctttag
    21301 aaagttgttt atgctctgct agatcattcg tcccaatgag aataaatact ttatctgctg
    21361 tatagctaag tacatctttg gcaaagcgtt ctcgtagagc ttgactgtta ttgccgctga
    21421 cagccgtatt cataatatga atcccagata ttttattttg gagatagaca ttcagcattg
    21481 gtttttttaa tgtttctttg cgaacaatca agctatttcc tatccaaaaa tactacctcc
    21541 tcgtcctgcc aagctcttag aattggatta ttcttagtat ccattagcgt ccatttattg
    21601 ctcgtgccag tcgattttga atcattctat ttaattgtaa cgaagttttt tagtttttca
    21661 actgtctttt caccgatacc agaaatctcc tgcaggtctt ccactttctg gaaggatccg
    21721 ttttcttctc taaatcgaat gatttcttgc gctttgactg atccgatccc aggaatctgc
    21781 tgaagttctg atggagtggc tgtattgata tttattttgg gaggctcgga atctgctgta
    21841 ggctgttttc cttccgagga tgccaactga tcaagatgtt ctggcagctc ttcacctaca
    21901 gcagggatat ataggacttg ctgatcttct atcttcgcag aaaagttgat tcgatcgcta
    21961 tccgcttctt ctaataatcc acctgcacgc tttagtacat catagacccg ttcttctgaa
    22021 gaaaaggaat agataccagg ctccttcaca cttcctttga tatcggtgta catgaccttt
    22081 ggacgagcat cctcttctac atcgtttcct tcagctgatg gatcttctcc actggaacta
    22141 aacgaagaaa gtatttcttt tgcagcagtc tcttctgttt ctctataaaa tagcaaattt
    22201 gataataaaa aaccacttgc gatacaaaag ataagaacta tcatagacat ccaaatgatc
    22261 aatttgggtg aagctagtat tttcttgctg attttttcta tcattgttct tttttcaaag
    22321 aatgaccagt aaaaatcatt cattactcct ttcatttaaa attgaagaaa cagtgagctc
    22381 tggcaaaatc gtcccttcaa catctaatta cgtcaatttt ttcttttgtt tttattttta
    22441 tcgtgaagat ttttgacgaa aaagaggggt tgtgacagaa ctcgtgtcac aacccctctt
    22501 taattattta gtttcatttc attttctcta gatagtcaag cgcttcttgt actgttttta
    22561 ctggtacgat tttcattgag gaattcaatt ttttagcggc agcttttgct tcttcgtagt
    22621 tggtttttat ttcaggatat tctttcttaa catcgtctga aatcgtatca tctggtgcaa
    22681 agaaaatctc tacgccagct ttatccgcac ttgccacttt tttatcgatt cctccgattc
    22741 ggccaacttc tccttcactg ttgatcgttc cagttcctgc aatttgctta ccatgtcgca
    22801 agtttttacc agtgacttgt tcatagattt ctaaagtgaa catcaatccc gctgatggcc
    22861 caccgataga gccagaatta atacgaacat ctgtatctga ttcgatctcg gtatggtcag
    22921 taagcgtgat acctattcct gcctttttat ctgttggtaa ctcgatcaga tcaccagaag
    22981 ctgttttttc ttcaccatta tgcaaataac tgatttcgat tgtttgtcca actttttggc
    23041 tttttacata atccataaat gcttctgtac tttgaaagct tttcccgtct acttttgtta
    23101 ctgtatctcc aactgaaagc ttgccaaaga aattcgatgt tttctctaca ctcatgacat
    23161 atacgccttt gaattccatt ttataaggct tgtctgcaag ttttaacgct tgttcgattg
    23221 ctgcattttg ggaagaatcc atataatact gttgcatttg attatattct tcatttgtcg
    23281 ccccaccagt caattcttct tcactgatca attcttcaaa aggggtcatt ttagctttta
    23341 aagctaagaa acctgtagct tgcctgatac caacagaggt aagagagaaa gacccagaac
    23401 ctttatcttc cttatcattg accgtaatta gttctttcag atcgatcgtg gctcccggtg
    23461 attctacata atacggcaag ggaactacta caaatccaat gatcaacatt ccaaatatga
    23521 taatcagtac tgttttcaac cattgtttag ctttcatttg attcacgctt tccagctaaa
    23581 gcttcgttga tcactggcgg taagtagctg ctcacatctc cgccaaagtg cagtacttct
    23641 tttaaaatac tggaactcac gtgactatat tctggttttg ctaataagaa gacagtttcc
    23701 aattcatcat atagatgatg attcatctga gcaatctctc gttcatattc aaagtctttg
    23761 atacttcgta tgcctctgat caacgcatct gcaccaatct cttttgctgt ttctaccgtc
    23821 aattgatttt cttgatgcat gacttttaca ttaggaagat gagaaacagc ttttgtgatc
    23881 aatgtcattc tttcttctgg gggaaacaaa tttttctttg aagtattgat aaaaacacca
    23941 attactacct catcgaataa tttagccgca cgttccaccg tatctaagtg accttttgta
    24001 aaaggatcaa aacttcctgg gaataatgca cgtttcattg atttccctct tttctataga
    24061 tagttacggc cgttatgcca taaatctgtt ttcttatttg atggaatcca gccacagttt
    24121 ctggcaattc gactgtttta tccgtttcgc atacgataac tgcggtttta gaaaaaagtc
    24181 ccagttcttc cattttatgg atttgatcga ttattttttg ttttgcatat ggcggatcaa
    24241 gaaacaagta gtcgaattgt tgtttctccg ctgctagccg ttcgattgct ttgtccgcat
    24301 caagttttat tgtggtaaat ttctctggtt ctttcgtaat ttcgatgttt tctttgatga
    24361 tttttagcgc ttggtaattt ttgtctacgc ataccgcatg actgcagcct ctagatactg
    24421 cttcgatagc taaaccaccg cttcctgcaa acagatccaa tactgtctct ccatcaaaat
    24481 atggtccaat catattgaat atggattctt ttactttatc tgtagttggt ctggtgttcg
    24541 cccctgctaa actttttaaa cggcgcccgc cataatttcc tgatattatc cgcatctact
    24601 cactccttag aaatggtact ggtactagta taccaaaaaa atacttggac aatgatcaaa
    24661 aatcgatcgt tatccaagtt attttttatt taaaaactgc tcataagaga tttttaaaga
    24721 tgttcagcta atcggatttc tgtatttagc tcttcaattt cgaaaccttc gtcttttaat
    24781 actgttttgt ttcctatttt ttcggcaaaa ttcatttcga tatccggtcg gtaagaacgt
    24841 tctacgtttc gaacaaaatg taatttacgg attcgttctt ctgtttcttc caatgtctct
    24901 tcatttaagt aaagcacgac atatttcatt cttcttgaga cataatgcaa caagccgaac
    24961 tttttcagat tctttaattg ttttaagctg tagacccata cgatcagccc gcgtcgtttt
    25021 gttatttcaa attcactatt gtctaattgc atgacaatta cctccgcatc caaagtgttt
    25081 tttcgtttca aaaaacgggc tacctgcatc tactttgatt tcattagaaa cttcttcggc
    25141 aagtacttgg acaatttgat ccaacagact ttgcagctgt gtttcactat atcggaaagc
    25201 tgcaacatgc tcatccatat caagctgtct ttttgctttt cgaacagctc gttttttttc
    25261 acgataatca ggtgcatact caccatatgc cgctacattt tcaaatgctt ctttcttaga
    25321 caagaacgct tctttcaact ggcaaacttt tgcagattct tccattgact gtttatgttc
    25381 aagataagaa gccatcgttt tactcttttt tatcatttca gcgacttgga ggcattgatc
    25441 ctccagttga aacagctctt cgttaacgat cactgctatc actactttct ccgttttctt
    25501 cttgcaataa gactaacata ttttctttag aaaaagcaat gcctacagaa tccgttcccg
    25561 ggtccatgaa acggtcatgg ttgtaaggat cgctgtacca atataaaaac gaaaaagtag
    25621 cgtcaatcgc cggatgaata aatagcccac tagtattttc taatttttgt ttactgatta
    25681 acttctccaa ttcatcattc gtcagtgaga atttcactgt cgtttgagca ctgcctagcg
    25741 atttttgcca atcagccata cgttcctctg acaatatttc ctcaggcctt gtcaaaaaat
    25801 catgtagtag cagctgactg tggttttgaa aggcaagtgt ttgattgtat atgggaagtt
    25861 cgtcttctct tctaagcata tttaataaag tcgtactttt tcgttcttta acgcgattga
    25921 ttgattccca atcagccgat ttatctgctt gataatgtgg aaggccttca ccaaacactt
    25981 gatatggcaa tagtttcaaa agactatctt tatctaaata cacaactgaa aataggccgt
    26041 ttgttgtttg atcaaaaaat aaaatagcaa aagtgtcgtt atcaaaagca attagcggac
    26101 gataattcat atcctcttcc attagttcga ttccaacttc tgtatcatgg tactcaaaag
    26161 tgaaattagt atagatagta gtgattttcg taagatcttg catcgtcatc cctaaataaa
    26221 acggcgcgat atcagcaggc cctgtttgca gtactttaac agttgtgact ctgctgtctt
    26281 ggatgttcgc ttcaaatgtg agatgactag ctttattttc ataatatctt gtttcaaaac
    26341 caaatccgct agttaatcga tcataggctt ggccaaaatc cttttccagc tgatcgatcg
    26401 gttttccgat ataagtagaa aaaccagttg ctttaagctc ctggtatttc cacgttgtca
    26461 tttggtttga tatttgttga tcagagtttt ccttcacttc aagttttggc ggaaacaaga
    26521 ctggttccat gtagaatgcc acaataacta atagcagaat cagccaaaac aaacccagtc
    26581 ttttcatgtt tccccacctt tcgcttatta tttctcagtc acttcaatca aaaggtcacc
    26641 agacgaaata gtgtctcctg aagacacata tacatgtgcg acttctccgt caaaacgtgc
    26701 ttcgatggcc gtttccattt tcatcgcttc agtaatcatc aacgtatcac cttttttaac
    26761 gttatcgcct tttttcacta atacatccaa aacagaacca gacatcgttg ctccaatttg
    26821 ttctcggttt gtcggttcag ctttcaatcg ggtttggacc gtactgatga tcgaattatc
    26881 tttgatagtg atttcacgtc tttgtccatt caaattgaag aacagtacgc gattcccttc
    26941 gatatcgggt tctccgattt catctaaacg aatgataagc gtttttccgc gttcgatttg
    27001 tacattgatt gtttcaccca aacgtatacc ttggaaaaat gttggtgtgt ccagtaatgt
    27061 aacatcgcca aattgattat atgctttttg atagtccaaa aagacttgcg gatacatcaa
    27121 atagcttaat acttcgtctt gttttggttc atagccaatt ttttctgcca attctttttt
    27181 tacttcgttg aagtcgactg attcagccaa aagacctgga cgttcattca atgcaggtct
    27241 gcctttaaga ataatctttt gcaatttttt aggaaatccg ccaacgggtt gccctagttc
    27301 tccttggaag aaagtcacaa cagattcagg gaaacttagt gtttcgcctt tttcataaat
    27361 atcttcttct gtaagatcat tttggaccat aaatagtgcc atatctccaa ctactttaga
    27421 tgatggagtt actttaacga tatcgccaaa catcaagttg acagtatgat acatttgttt
    27481 gatttcatcc cagcgatgac ctaatccgac agcttttgcc tgttgctgta gattcgaata
    27541 ctgtcctcct ggcatttcat gcatatatac ttctgtttca ggagcattca agccgttttc
    27601 aaacggtttg taatacatgc gcacatcttc ccaataatgg tttaatttct gtgcattttc
    27661 aatcgtgatt tctggcagcc gtggaccatt gaccagtgca taatacaagc tgctcatgct
    27721 aggctgacta gtattcccac tcattgcact cattgccacg tcaacgatgt ctactcctgc
    27781 ttttgaagca gcagaataag tgatgattcc attcccgctg gtatcatgag tatgcagatg
    27841 gattggcaga tctgtcgttt ctttcaattc actaatcaaa cgataagctg cctgcggttt
    27901 aagcagccca gccatatctt tgattgcaat gatatgcgca cccagttgtt ctagttcctt
    27961 tgccatgtct ttataatact gaacattgta ttttgcccga cttggatcgt tgatatcgcc
    28021 agtatagcaa atagctgcct ctgcgatttt accagtatcc cgtacagctt ggatactttt
    28081 ttccatttgt ggagtccagt tcaaactatc aaagattcga aatacatcga ttccttgcgc
    28141 tgctgcttct ttgacaaatt ccaccaatac attgtcagga taattagaat acccaacggc
    28201 atttgaacct ctgaaaagca tttgcagcaa cgtattaggc attaaagaac gaatttttct
    28261 caaccgttgc caaggatctt cattcaaaaa gcggtaagcc acatcaaatg ttgctccccc
    28321 ccacatctca cttgaaaaga gttcaggaag accttctcca gtcaaacgtg caatctgctt
    28381 gaaatccttt gtccgaacac gagttgctag cagactttga tgggcgtctc taaatgtcgt
    28441 atccgtcaac aacaaatttt cctgtccttt gatccattcg accaaagcat cggcaccctt
    28501 cgcatctaat acattcttag ccgttacgta atcagttctt gtgattagat ctttaggcat
    28561 acgtggttct tcataaaatg gtttttcgcc actttcgata cctggaaaac cattcacagt
    28621 aatttcaccg atatatttca tcgttttgtt tccgcggtct cgtagtcgag gaaattcaaa
    28681 taatgttgct gtactatcaa tgaaggttgt ttttgcattt cctgattgga attcaggatg
    28741 tgtgatgaca ttgagcataa atggaatgtt tgttttcact ccacggattc taaattcttt
    28801 caagcaacgt tccattttct gaatggctgt ttcaaaggta gcaccatgcg tacatacttt
    28861 tactagcaat gaatcaaaat acggtgtcac tacatagccc gcgtaagcat ttccgacatc
    28921 cagacgtacg ccaaaaccac caggtgaacg atacgtatcg attttccctg tgtctggtaa
    28981 aaagttattc aacgggtctt ctgtagtgat tcggcattga atggctgaac catttaattt
    29041 aattccttct tgctgaggca atccgatttc tttatgcaga tccaaacctt gagcaatcaa
    29101 cagttgcgtt gttacgatat caatatctgt aatcatttct gtgatcgtat gttccacttg
    29161 gacgcgaggg ttgacttcga taaagtaaaa atcatctcct tcaacaagaa attctactgt
    29221 acctgcgttg acatacccga catatttcat caattggaca gctgcctggc agattttttg
    29281 acgttgctgt tcattcatag agacacaagg cgctacttct actacttttt ggtgacggcg
    29341 ctgaacagaa caatctcttt caaaaagatg gatgacgttt ccatgcgtat ccccgagaat
    29401 ctgtacttcg atatgcttag gattagcgat gtatttttca acatacactt catcactacc
    29461 aaaggctgct tttgcttcgc ttttcgcacg ttcgtagccc tctcttgcac tcttttcatc
    29521 gtgagctaca cgcatgccgc gtccgccgcc gcctagagct gccttgatca taatcggata
    29581 accgtattgt ttcgcaaact ccaaggcatc gtctatagaa gcgattggtc catctgtgcc
    29641 tggaatcgaa gcaatccccg cttcgatagc tgcagctttt gctttgattt tatctccaaa
    29701 aatatctaaa tgatgcagtt tgggtccaac aaaaatgatg ccttcttctt cgcaccgttg
    29761 cgcaaaattc aagttttccg acaaaagacc gtaacccgga tgaatagcgt cagctccgca
    29821 ttctttcgcg atagaaatga tcccttcaat atctagatac gcatcaatcg gtttttttcc
    29881 ttgtcctact aaatatgctt catccgcttt gaaacggtgt acggaatatt catcttcttc
    29941 tgcatagatt ccaacagttt taattcctaa ttctgtacaa gcacgaaata cacgtacagc
    30001 gatttcaccc ctgttagcaa ctaaaacttt tttcatcgaa ccactcctac atcttttttt
    30061 attataaacc taagctcttg cgtttttcat ccgcactgat gttgaggaca aagccgacac
    30121 aaatcgaaag catcagcaaa ctggagcctc cttgacttaa aaatgggaac gtgatccctg
    30181 tcaaaggaat aacgccagta attcctccaa gattgacaaa tacttggatc aaaaacattg
    30241 cgccgatccc aatgcacaaa agcgaattga acggatcttt tgaacggata cctacaagga
    30301 caatgcgcac aatcataaag aatagcaacg ctaatatcaa aatccccata atcactccca
    30361 gttcttccac tacgattgca taaataaaat cggtttgtgc ttcttgtaaa aatccttttt
    30421 tctggatact gttacctaat cccctgccaa atagtcctcc gttgaacatg gcatagtagc
    30481 cgttgactag ttgatgccct ttatttaatt cgtctgagaa aggattttgg taaatagcaa
    30541 atcgattata gatatactgt aaacgtccgg gtaagatctt tccgtttgtg atattgatca
    30601 gccagatcgt aaaagtactt agacaaaagc ctcctactcc tacaatcaaa gtatacacat
    30661 aattgactcc gctggctaaa agcagaacca aaataatcaa aaagataact gccgcattac
    30721 caaaatcggg aagaatagcg actaatgcag taagtccgat aaccattgca agtggacgtt
    30781 tcacagtccc aagaaaatct ttttgtacac tgttttgtct tttagataaa gtcagagaca
    30841 ggtaccagat agaaatgatt tttaggtatt cggcaggttg gatcgagaat cctgcaattt
    30901 gcagccaacc tttcgcccca ttgatctcct taccaaaaaa cacaacaatc agcagcaaaa
    30961 tcgtcaaaac tgcgatggct gccatgatca aacgctggtt tttcaaaaca tctgttttca
    31021 ttttataaag tagtgcgata gcaatcaagc taagcaccca aaaaatactc tggttaatga
    31081 ctgatgcgga cggattttgt ccgttttcta ataatagata cgacgtagaa ctatatacca
    31141 tgatcaatcc caatacacat aaaattaaat agggaatcaa gatgctatag tctaatagat
    31201 gccttttttt cactttctta ggcaagtcta ttcctccttg ccaacttctt ttttatcata
    31261 aatttcttgg taaacctcac tatataactg attcaattct cgttctagct cacttaacat
    31321 cagctggccc tcgttctttc cgattactcc tatacggaca gcaaaaccga cttgccggga
    31381 aaagccgtac atctgtgtat ctaccacttc ttcgaaggct ttgcattgtg agatacagag
    31441 actgttgttt tgatttcgga ttaatcgttt gatccgttct gcttcatcat ttaatacttg
    31501 gatcgcaagt tttttcgaca aagaatccat ctgtgcttac ctcccattct aattccttaa
    31561 ttataacata tttgattttc ataaggagca aaaaaaacag atttaaccag ttgtttttat
    31621 tcatttttac gtattcgttc tttctactta tccagaacgt tagagtccgc attttttatc
    31681 cgagataaaa aggcttcgcc atatttatca agcttgcttt ggccaactcc ttttatctga
    31741 agcatctcca atctgcttga tggacgtatt cggctcattt ctttcaatgt ggaatctgaa
    31801 aatacgacat acgggggaac gcctgcttct gatgctagat ccgttcgcag ttcccgcagc
    31861 acttcaaata gtgtatcggt ttcttcatcc gatagctgct ggactttctt gggttccttt
    31921 cgataaacag attcttgtcc tttcaatact tgtatccctc tttctgttac tttcaaaaca
    31981 ggatactcac cattgatggt cagaagatag ccattcgata tcaaatattc aattaactgc
    32041 atcgtttctt tttgcgattg gtttttcatc agtccatagg tagataaatg tccaaaacct
    32101 aaagcttgca gcttttgttc tttcgatcct gctaagactt tcatcagcat ttgtttacca
    32161 tagttctcac ccatacgttt taagcaagaa agaaccatct gtgcttctgt cgttacttct
    32221 actatttctc tgtcatctaa acagttacca caacggttgc acggactgct tgtctcgcca
    32281 aaatagttta ggatgtattg ctgaagacag gactcaatat acacgtattc tgtcatcgct
    32341 tttaattttt catattcttt ttgcttttgc ccttcttctc tttgggactg ctggataaaa
    32401 aacttttgga cttggatatc ttgaggagag aaaagcagga tggcttcact ggaaagacca
    32461 tctcttcccg cccgaccagc ttcctgatag tatgcttcta gagagccagg aatttgataa
    32521 tggataacaa aacggacatt gcttttattt atacccatac caaatgcatt tgtcgctaca
    32581 atcagctgca agcgatcgta taaaaaggct tcttgcattt ctgttcgctc attctcattt
    32641 agaccaccat gatatctacc cacggaaaaa tcgaattttt taagcaaatg atagagacgg
    32701 tctacttctt ttctcgttgc tgcataaatg ataccagatt gatttttgtt gatctttaaa
    32761 tattcgatta gatattgttc ttttttttga tctttaatta cttgaaatcg tagattttct
    32821 cgttcaaaac cagtctgaat atggttgttt tctggaattt ttaaaagtct ttttatgtca
    32881 gcagctactt gcacagtcgc tgtagctgtc aaagcaacaa ttacaggacg atttggcagt
    32941 tgatctagct ggttcgccat ctgtaaataa cttgggcgga aatcatgccc ccactgagag
    33001 atacaatgtg cctcgtcaat agcgatcata cggacgttcc atcgcttcat tgcttggata
    33061 aaatccggca tgataaaacg ttcaggagcg atataaagta aacggtactg ctgtctatcg
    33121 atttcttgga agcgtctagc tgtttcgtaa ccatctaacg agctattgat aaaagtggcg
    33181 ggtataccta attgattcgc cgcgtccacc tgatccttca tcaaagagat taaaggggac
    33241 acaaccacag taagcccatc taatagtaaa gctggaagct ggtaacagat tgatttccca
    33301 cttccggtag gcatgattcc taatacattt tcctgatcaa tgactttctg gataatttct
    33361 ttttgaccag gtctaaactc atcatagcca aagtattgtt ttaataagtc gttcatcttc
    33421 ccgctccttt ctcttgccat tttacttcga ttttcttcga aggacaagtt gatctccttt
    33481 cgtgaaacat tatcttatta tgaaacaaaa actctctaga aataagtttt ttaaaagtct
    33541 tttaaacgtc tttcggcgta ttttctgaat ttatgtccat tgacaaagat gacttctcca
    33601 cagaaaagag ttggacaaaa aaagaaacca gctatt
//
LOCUS       DACDNO010000028        32532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDNO010000028 DACDNO010000000
VERSION     DACDNO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.028"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            130..504
                     /locus_tag="IWI22_001392"
     CDS             130..504
                     /locus_tag="IWI22_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9168792.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(618..1247)
                     /locus_tag="IWI22_001393"
     CDS             complement(618..1247)
                     /locus_tag="IWI22_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9168793.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1381..1453)
                     /locus_tag="IWI22_001394"
     tRNA            complement(1381..1453)
                     /locus_tag="IWI22_001394"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1418..1420),aa:Thr,seq:ggt)
     gene            1667..1740
                     /locus_tag="IWI22_001395"
     tRNA            1667..1740
                     /locus_tag="IWI22_001395"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1701..1703,aa:Asn,seq:gtt)
     gene            1695..1844
                     /locus_tag="IWI22_001396"
     CDS             1695..1844
                     /locus_tag="IWI22_001396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168794.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2337..3665
                     /locus_tag="IWI22_001397"
     CDS             2337..3665
                     /locus_tag="IWI22_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9168795.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3867..4700
                     /locus_tag="IWI22_001398"
     CDS             3867..4700
                     /locus_tag="IWI22_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9168796.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4716..5453
                     /locus_tag="IWI22_001399"
     CDS             4716..5453
                     /locus_tag="IWI22_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9168797.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5608..6576)
                     /locus_tag="IWI22_001400"
     CDS             complement(5608..6576)
                     /locus_tag="IWI22_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9168798.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6808..7641
                     /locus_tag="IWI22_001401"
     CDS             6808..7641
                     /locus_tag="IWI22_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9168799.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7675..8514
                     /locus_tag="IWI22_001402"
     CDS             7675..8514
                     /locus_tag="IWI22_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9168800.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8540..9100
                     /locus_tag="IWI22_001403"
     CDS             8540..9100
                     /locus_tag="IWI22_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9168801.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9299..11020
                     /locus_tag="IWI22_001404"
     CDS             9299..11020
                     /locus_tag="IWI22_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9168802.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11185..12327
                     /locus_tag="IWI22_001405"
     CDS             11185..12327
                     /locus_tag="IWI22_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9168803.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12343..13554
                     /gene="thiI"
                     /locus_tag="IWI22_001406"
     CDS             12343..13554
                     /gene="thiI"
                     /locus_tag="IWI22_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9168804.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14127..14774
                     /locus_tag="IWI22_001407"
     CDS             14127..14774
                     /locus_tag="IWI22_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9168805.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            15167..17812
                     /locus_tag="IWI22_001408"
     CDS             15167..17812
                     /locus_tag="IWI22_001408"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9168806.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18122..19435
                     /locus_tag="IWI22_001409"
     CDS             18122..19435
                     /locus_tag="IWI22_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9168807.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19425..20078
                     /locus_tag="IWI22_001410"
     CDS             19425..20078
                     /locus_tag="IWI22_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9168808.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20133..20825
                     /locus_tag="IWI22_001411"
     CDS             20133..20825
                     /locus_tag="IWI22_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP9168809.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20975..21175
                     /locus_tag="IWI22_001412"
     CDS             20975..21175
                     /locus_tag="IWI22_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9168810.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21429..23108)
                     /locus_tag="IWI22_001413"
     CDS             complement(21429..23108)
                     /locus_tag="IWI22_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9168811.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23110..23322)
                     /locus_tag="IWI22_001414"
     CDS             complement(23110..23322)
                     /locus_tag="IWI22_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9168812.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23715..25445
                     /gene="efrA"
                     /locus_tag="IWI22_001415"
     CDS             23715..25445
                     /gene="efrA"
                     /locus_tag="IWI22_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9168813.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25442..27202
                     /gene="efrB"
                     /locus_tag="IWI22_001416"
     CDS             25442..27202
                     /gene="efrB"
                     /locus_tag="IWI22_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9168814.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27297..27494
                     /locus_tag="IWI22_001417"
     CDS             27297..27494
                     /locus_tag="IWI22_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168815.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27647..28132
                     /locus_tag="IWI22_001418"
     CDS             27647..28132
                     /locus_tag="IWI22_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9168816.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28236..28829)
                     /gene="clpP"
                     /locus_tag="IWI22_001419"
     CDS             complement(28236..28829)
                     /gene="clpP"
                     /locus_tag="IWI22_001419"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9168817.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29009..29536
                     /locus_tag="IWI22_001420"
     CDS             29009..29536
                     /locus_tag="IWI22_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9168818.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29627..30364
                     /locus_tag="IWI22_001421"
     CDS             29627..30364
                     /locus_tag="IWI22_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9168819.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30368..31285
                     /gene="miaA"
                     /locus_tag="IWI22_001422"
     CDS             30368..31285
                     /gene="miaA"
                     /locus_tag="IWI22_001422"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9168820.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31287..32516
                     /gene="hflX"
                     /locus_tag="IWI22_001423"
     CDS             31287..32516
                     /gene="hflX"
                     /locus_tag="IWI22_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9168821.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10771 a         5522 c         6875 g         9364 t
ORIGIN      
        1 tacactctga gatgtaagaa atatcataag atatttctta catctttttg ttttgagcag
       61 gactttagat aattttatta atatatgcta gcatgctttt gtaagaaaaa gaaagagggc
      121 gtattagtta tgtatcttaa aattacggaa caagcacaag aaagattaca aaaatatatt
      181 gatgaaggcg caacagttat attggattta gatgatggcg ttggggaata ttctaaaatg
      241 ggtgtttgct ctttagatac tagttttcgt ttacttcttc ttgataaaga acagagaaaa
      301 aatgattata aactccaatt agatagtgat attggggatg tttatataaa agattattca
      361 aaaatgtaca tggatgaaca tatgaccctt tctttagatc cacgtctagg tgtatttact
      421 cttgaaagcc caagtggttc tttagacagt catgttcaat tagtagatct acgaccagaa
      481 aaattctctg gaataaaaca ataactttct ctctattgtc cataaaacta gtctcaatca
      541 agctgtattg aaaaaacaag acaacattct taccatggta aataaaaaga cgacacattt
      601 tgtcgtcttt ttttaaatta actattttcg ataaaagatc gcatagcttg tgccattatt
      661 tctggctttt ctctattcaa ttcatgtttt ccgtttttaa taaagagtag ccggccattc
      721 gttgaaagtt tatataaacg ttttgctgct ggtttattcg catgatcttt ggtaccgcat
      781 aaaatcaatt taggaagtcg tgatgtacga agctgttttt tgcagtctaa tgtttcaagt
      841 gaacgaagta gggtaagggt ttgtttttta gatagaccta atttttcaaa tatcttagaa
      901 ggtacgcagt aaaaataaat attctggaaa gtaagcaata agcgattagg agactcaaat
      961 tgtggagcga ctaaaacaac cttttttaaa tatggagaag gagttgcaag atagtttaaa
     1021 gcaataacag cgcctaaaga taatccacat aaaataaaag gttcctttat ctgattcaat
     1081 tgtacttcga tgtatttggt taagtcgtct aaagtagcag tgtctgtgct gtttaaatga
     1141 ttgaacaatg aaagactttt gcaagaataa ctattgggta gataagagat tacatctttc
     1201 catgattgtt cgtcttggcc tagaccatgc aaaaaaacaa ttttcatatt gctctccttt
     1261 ttatagttat catatagact ttctttgtac tattaaaata taaacattca tttcttacgt
     1321 cataaaataa aaaaacagcg cagacacatt gttaaaccaa catgttgcac tgtttaagga
     1381 agccacttgc cgggcttgaa ccggcgacct cttccttacc atggaagcgc tctaccagct
     1441 gagctaaagc ggcattatca ttaatacaaa taataggata acgaatatta atgtatttgt
     1501 caacagaatt ttttcgaaaa aatgtgtttc tctaatagga aaacagattt tagaaaaaat
     1561 aaaaataaca aaaaatgcga acggtttctt tgataatata ataaaaaaat tcaaaaaaag
     1621 tattgcgtta tttagaagaa attgatatta tattcatgta cttgattccg gcatagctca
     1681 gttggtagta gcgcatgact gttaatcatg atgtcgtagg ttcgagtcct actgccggag
     1741 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
     1801 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
     1861 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
     1921 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
     1981 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
     2041 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
     2101 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
     2161 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
     2221 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
     2281 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
     2341 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
     2401 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
     2461 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
     2521 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
     2581 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
     2641 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
     2701 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     2761 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     2821 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     2881 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     2941 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     3001 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     3061 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     3121 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     3181 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     3241 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     3301 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     3361 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     3421 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     3481 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     3541 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     3601 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     3661 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     3721 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     3781 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     3841 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     3901 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     3961 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     4021 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     4081 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     4141 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     4201 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     4261 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     4321 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     4381 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     4441 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     4501 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     4561 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     4621 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     4681 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     4741 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     4801 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     4861 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     4921 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     4981 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     5041 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     5101 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     5161 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     5221 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     5281 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     5341 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     5401 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     5461 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     5521 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     5581 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     5641 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     5701 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     5761 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     5821 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     5881 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     5941 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     6001 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     6061 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     6121 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     6181 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     6241 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     6301 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     6361 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     6421 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     6481 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     6541 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     6601 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     6661 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     6721 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     6781 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     6841 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     6901 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     6961 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     7021 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     7081 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     7141 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     7201 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     7261 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     7321 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     7381 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     7441 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     7501 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     7561 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     7621 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     7681 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     7741 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     7801 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     7861 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     7921 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     7981 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     8041 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     8101 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     8161 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     8221 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     8281 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     8341 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     8401 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     8461 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     8521 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     8581 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     8641 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     8701 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     8761 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     8821 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     8881 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     8941 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     9001 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     9061 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     9121 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     9181 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     9241 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     9301 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     9361 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     9421 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     9481 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     9541 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     9601 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     9661 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     9721 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     9781 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     9841 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     9901 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     9961 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
    10021 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
    10081 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
    10141 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
    10201 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
    10261 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
    10321 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
    10381 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
    10441 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
    10501 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
    10561 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
    10621 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
    10681 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
    10741 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
    10801 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
    10861 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
    10921 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
    10981 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
    11041 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
    11101 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
    11161 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
    11221 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
    11281 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
    11341 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
    11401 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
    11461 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
    11521 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
    11581 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
    11641 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
    11701 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    11761 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    11821 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    11881 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    11941 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    12001 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    12061 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    12121 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    12181 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    12241 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    12301 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    12361 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    12421 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    12481 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    12541 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    12601 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    12661 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    12721 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    12781 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    12841 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    12901 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    12961 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    13021 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    13081 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    13141 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    13201 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    13261 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    13321 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    13381 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    13441 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    13501 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    13561 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    13621 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    13681 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    13741 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    13801 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    13861 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttgcgaa
    13921 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttac
    13981 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgttttat ttgtctcaca
    14041 cccttttcct tcctacttgt aaccttgctc ccgtgttaga aacatgttac actaaatatg
    14101 tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa
    14161 ctgcgaaaag attaccttta tatttacgct acttgaagat gctggatgac tcaggtataa
    14221 gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat tccttctgca acgatccgca
    14281 gagacttctc gcaattaggc gagctaggca gaagcggata tggctatgat gtcccttttc
    14341 ttatcgatgt attcaataat atccttaata ctaaagaaga aaaacgaatc gcattagtag
    14401 gatatggaaa tcttggcaaa gcattgaaac acaacaattt tagaagaaat gaaaatctaa
    14461 atatcgtgtg cgtctttgat aatgatccag cactgatcaa tcgagtaatt gatggagaaa
    14521 tgatctatcc gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa
    14581 tctctacggt accaagtaaa tattcacaat ctgctattga tgaaattgtc aaaggaaatg
    14641 taaccgctat acttaatttt gcgcctgaca gagtaactgt cccagcttat gtcaatgttc
    14701 aatacattga tttgacaacc gagttgcaga cgcttattta ctttgatgag aattactcag
    14761 aagttttttc ttaggaaaat ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt
    14821 ctttcaaaaa ttttttagta ctaaatgaag tggttttttc acgaccactt gttgactaaa
    14881 aaaaaaaacc attgtagaat gaagcataac gctgaacaag aggagtaact ggcacattca
    14941 ttcttagaga gagaactatc tgctgaaagg ttctcaatga caaccagtga aggtagcttg
    15001 ggagtttctg tcttgaatgg agtagagata gacgagtcgt gatcgttaac tcacttgaga
    15061 ggcaataatg attgattatt gcaatttagg tggtaccgcg taaatcatac gtcctagaat
    15121 acgaaagtat cttaggacgt tttttagttt aaaggaggaa aacagcatga cggaagaaaa
    15181 taacctatca acaaaatata atccgcaaga agttgaagct ggacgttatc agcattggtt
    15241 agaacaagat ttgtttaaac cgagcggaga taaaaaagca aaaccttatt caatcgtaat
    15301 cccgccccca aatgttactg gaaaacttca tttagggcat gcttgggata cgaccttgca
    15361 agatatgctg attcgtcaaa agagaatgca aggatatgat actttatggc tgcctggtat
    15421 ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg aagcttagag agcaagggat
    15481 ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga
    15541 gtatgcgagc cacatccgcg aacagtgggc taaaatgggc ttatctttag attacagtag
    15601 ggaacgtttt acattagatg atggattatc tgaagcagta cgtaaagttt ttgttacctt
    15661 atacgaaaaa ggtttgattt atcgtggtga atatatcatc aactgggatc ctcaagcacg
    15721 aacagctcta tctgatatcg aagttattca taaagatatc gaaggcgcct tttatcatat
    15781 gagctatgaa ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac
    15841 gatgttggga gatacggcga ttgctgttca tccagaagac gagcgttatc aagcgctcat
    15901 cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc ccaatcattg cagatgaata
    15961 tgtagatatg gaattcggga caggtgtagt aaaaatcact ccagcacatg atccaaacga
    16021 ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg atgatggtac
    16081 gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt tttgctgctc gtaaagcaat
    16141 cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga tccacagtgt
    16201 gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt
    16261 aaaaatggcg ccgttagcga aaaaagccat tgataaccaa gaaacagatc aagcagtgga
    16321 attcttccct ccaagattca atcaaacgtt tttacgttgg atggaaaatg tccatgattg
    16381 ggtaatctca cgacaattat ggtggggaca tcaaattcct gcatggtatc acaaagaaac
    16441 aggtgaaatg tatgtaggga tggaagcacc agcggatagt gaaaactggg tacaagatcc
    16501 tgatgtttta gacacttggt ttagttcagc tttgtggcct ttctcaacaa tgggctggcc
    16561 agatacggaa agcgaagatt ataagcgata cttccctact agcactttag taaccggtta
    16621 tgacatcatt ttcttctggg taagcagaat gattttccaa agcttggaat ttactggcga
    16681 acgtccattt gagaatgtct tgattcatgg attgattcgt gacgaacaag ggcggaagat
    16741 gagtaaatca ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt atggagcaga
    16801 tgctctccgc tggttcttat cgaatggttc tgcaccagga caagatgtcc gcttcagcta
    16861 tgaaaaaatg gatgcttctt ggaactttat caacaaaatc tggaatgctt cacgtttcgt
    16921 tatcatgaat atcgaaggaa tgaccgtaga agagatcgat ttatctggag aaaaatcagt
    16981 ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt
    17041 tgatcgtttt gaatttggtg aagcaggtcg acaactgtat aactttatct gggatgattt
    17101 ttgtgactgg tacatcgaaa tgagtaaaga agtactttat ggagatgatg ctgtttcaaa
    17161 acaaaccacc cgtagcatct tagtccatac acttgatcag atcctacgtt tattgcatcc
    17221 aatcatgcca ttcgttacag aagaaatatg ggaacatatt cctcatcagg gaaattctct
    17281 agtagtggca gaatatcctg ttgtacgtcc agagtttgat gatgaaacag cttctaaagg
    17341 gatggaagta ttgaaagaat tgattcgagc cgttcgaaat attcgttcag aagtgaatac
    17401 accattgtct aaaccaatta cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt
    17461 tttagttgaa aatacaagct atatcgaacg cttctgtaat cccgaagagt tgacgatatc
    17521 aagtgagatc actgcgccag atctagcgat gtcagctgtt ctgacaggtg cagaaatctt
    17581 cttgccgtta gcaggattga tcaatattga agaagaaatc aaacgattag aaaaagaact
    17641 ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt
    17701 ttctaacgcg ccggaagaag tagtagaggc agagcgtgca aaagaaaaag attatttaga
    17761 gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt actattcaat aagaatagaa
    17821 aaagagagtg tgaaatcagc tagctattac taaaagctag ctgattgaac atcgttacag
    17881 gaaagagttt gagcctcact cttagcttcg attcataagg agatgtgaca taaattttgt
    17941 cgcatctctt ttttagtcta ctcttattat gtaggagaag gagggaggtg gatcatgtat
    18001 caagagtcaa taaagtagtt gataaaatcg ttcctctctc tgtttctttt ctctttgtca
    18061 tacttttatt tttcttatgt gttctatttg ttataataaa agagaaatgg gggaattgtt
    18121 gatgacgatt gaagaagcaa tagaatggat ccatagtcga ttaccatttg gttcacgtcc
    18181 tggccttgac cgagtcgaag ctttattaga agtggtaggg catccagaga gaaaagtaaa
    18241 aacgatccat atcgctggaa caaacggaaa aggttctact gtagcttatt tgagaagcct
    18301 attagaagag gctgggctga cagtaggtac atttacttca ccttatatcg aaacgttcaa
    18361 tgaacggatc gcaatcaatg gcaaaagcat tccagacaat gatttgatcc agcttgtaaa
    18421 aaaatatcag ccactgatca aacaacttga tcaatttgaa gcagtagctg gtattactga
    18481 attcgagaca ttaacagcaa tggcatttga ttattttttg atgaagaaag tggacgtagc
    18541 gattatcgaa gttggcttag gcggcttatt ggattctacc aatgtagctg taccaatgct
    18601 gacgggaata acaacgattg gacttgatca taccgaaata cttggagata caattgaaga
    18661 aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat
    18721 tgccccagaa gcttttactg tcatccaaca aacagctaaa gcaaaaaatg caccgcttta
    18781 tgtatacggg gaaaattata aaataaacta tctgcatcct gatgagatgt ggggagaagt
    18841 atttgaattt tttaatgaaa taggaaagat ccctcaatta accactccgc ttttagggca
    18901 acatcaagct gaaaatgcag gagtagccat cgaattgtat tttgtttact gtcaattgga
    18961 aaagctgcct ttccgagaaa aagatgtttt gaagggattg aagaaatctc gatggccagg
    19021 aagaatggag cgtttgagta acgagccatt agttgtttta gatggtgccc ataatacgca
    19081 tgcaatgaac cggcttgttg agaatttgaa aaaagaattc aaaggatatc aaatcaacat
    19141 tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt
    19201 accaaatgtc catatttatt tgacgacctt tgaacatcca aaagccatcg aattaagtga
    19261 agtatcagat ttagcaagtg agagagtatc aatcgtttca ttgtggcaat tcggattagc
    19321 agatatattg gaaaagatga caaatgaaga cgtattagtt gtgaccggtt cactttattt
    19381 catttcagaa gttcgccatt tattaataca attaggagga gatcatgaag tataatgcag
    19441 tgatttttga catggatggt ttgctgttcg acacagagtt agtgtattac gaagccacac
    19501 aagtagtagc agacaagatg ggttttcctt atgataaaga cctgtattta aaattcttag
    19561 gtgtttcaga tgaagaagta tgggcgaatt atcatatgat tttctcggaa tttggcaaaa
    19621 caagtgtaca aagattcata gatgattcat accaggaaac gcttgaacgt ttttcgcaag
    19681 gagcggtaca aatgaagccc ggtgtgttgg aactcctctc ttttttagaa gaacatcaga
    19741 ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat tgaactttta ttgaagaaga
    19801 acaatctgtt tgaacgattt gagacgattg tttctgcaga aaatgttcaa cgagcaaaac
    19861 cagacccaga aattttcttg ttagctaatc agaagctagg tacagcgaag aaagaaacat
    19921 tgattttaga agattcacaa aatggtattt tagcagcttc tgcggctgag atcccggtag
    19981 tcatggtacc agatctgctt ccaccatccc aagaattgat caaaaaaaca gctggggttg
    20041 tttcttcttt acatgaaatc cctgcattgt taaaataaaa atccatctct tttcgtatct
    20101 tattttagat aagatagaaa ggagatgttt ttttgggaaa acgattgatc aaagaagtac
    20161 ctactagctc tttaccaagg gaacgtatgg aaatttatgg cgcggaagct ttatcggatc
    20221 aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg
    20281 ctggaaatct gttaaaaacg tttggaggac tggcctcttt acgacaggct actttgcatg
    20341 agttagaaga gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg
    20401 aattaggtcg acgaattcag acggatcatg aaagagcggc tccaccggtt cgtaccagct
    20461 acgaattggc ccagcaattg attctggaaa tgaaagatca caaacaagaa catttggttt
    20521 gtatttattt ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc
    20581 tgaatcagtc agtcgctcat cctagagaaa ttttccatta tgctgtacgt tattgtgcag
    20641 caaggatcgt tttagcgcat aatcatcctt ctggtaatgt tattccatcg cagcaggata
    20701 tgaattttac caaaaggata caaaagtgtg gagaaatgat ggggatcact gtactggacc
    20761 acttgatcat cggacgcaaa cgatatttta gtttgagaga agaaggaatg atggaagaga
    20821 agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac aattatattg taattttgtt
    20881 tgggaataga agttgaaaca tctgttgcac ctctatcgta cacgagcaat gttacaattt
    20941 aaattagtgc ttttggcacg aggaggatat tattatggaa aacggaacag taaaatggtt
    21001 caacgctgaa aaaggatttg gatttatctc acgtgaagat ggaagcgacg tattcgttca
    21061 cttctcagct atccaaggtg acggattcaa aacattagaa gaaggtcaag cagtgacttt
    21121 cgacgttgaa gaatcagacc gcggcccaca agctgcaaac gttgtaaaag cttaatttta
    21181 tctgtaaatc aaattgatgg tagacatcaa tttttacgag agaacaaagc gagtaacaaa
    21241 aatacctgcc acaagagaca atttctcttg tggcaggtat ttttttgtat gaaaaagtaa
    21301 ataaatttaa gaggctatga aagagccacc ttgtatcaag gaataagaac aaacaaaaat
    21361 ggcttctcat gtttttcatt tgttttctct tatattcagg acaaggtgct gatttcataa
    21421 cctcttattt attcttcatc tggtgtcatt accattggca aaatcattgg atgtctttct
    21481 gtacgttcga ataagaaagg ctgaagcgcg ccagtcatcg cttctttcaa tttatattct
    21541 gaacagtcag gtccattcaa acattctctt aaagcgtgga ataagattcg ctgagcttca
    21601 ttgatcattt ctcctgattc tcgcatgtag acaaatccac gagagagaat atctggacca
    21661 gccaagattt cttttttctt gatatccact gtcgcgacag ctaatacaag accttcttct
    21721 gaaagcaggt gacggtcacg taaaacgaca ttaccaatat caccgatccc gctaccatcg
    21781 atatacacat ctccagccac aaaatgccca gcaggacgcg cagaatcgct agttaacgct
    21841 aaaacatcgc cattttccat gatgaagcag ttttctgcag gaacacctac atcttgtgct
    21901 agagaagcat gtactttcaa catgcggaat tctccgtgga caggaataaa atattttggc
    21961 ttcatcaaac gaagcattaa tttttgttct tcttgtccac cgtgaccgga agtatggatg
    22021 ttattgattt ttccgtgaat cacttctgca ccagcttcag acagcaaatt gatcaaatga
    22081 ttgacacttg ttgtattacc aggaataggt gaacttgaaa agatcactgt atcgtctggc
    22141 tggatttgga tttgacggtg agtaccattc gcgatgcggc tcaacgcggc catcgcttct
    22201 ccttgagaac ctgtacatag gatcatcgtt tcattggcag gcaagcgatt gatttcgtgt
    22261 gcatctacaa atgtgccgtc tggaactttg atatatccta aacgttgacc gttgacgatg
    22321 gcattttcca tgcttcgtcc gaaaacagcg attttacgac cagttgccac agcggcatct
    22381 gctgcttgct gcaaacggaa gatgttagaa gcaaagctgg caaaaatgat acgtccttcg
    22441 atttttgaaa agatcttcaa aatagaatgc ccaatcgttt tttctgattt agtaaatgtt
    22501 ggaatctcag catttgtact atcagataaa aggcacaaga ctccttcttc cccgatacga
    22561 gccattttat gcaagtttgc tggttcgcca acaggagtga aatcgaattt gaagtctcca
    22621 gtcgctacga tatttccaga aggtgttttc accacgatcc ctaatgcatc tgggatacta
    22681 tgggtcgttc tgaaaaagct gacagaagtc tttctaaaac ggatgatcgt atcttcattg
    22741 atctcatgga gctctgcatc tcgaagcaag ccatgctcat ctaatttgtt tgtgatcaac
    22801 gctaatgcga gaggaccagc atagatagga atatttgctt gtttcagcaa gtagggtatt
    22861 ccgccgatgt ggtcttcgtg cccgtgagta atcaccaagg cttttacttt gtgtaagttt
    22921 tgtatgatgt agctgtagtc gggaatgaca tagtcaattc caagtaaatc gtcttccggg
    22981 aatttgatcc cggcatcaat caaaatgatt tcatcttgga attgtacccc atacgtattt
    23041 ttcccgattt cacctaatcc accaatagca aagacgcctg tctcgttatt tttgatggaa
    23101 agtttcatat taaaactcca ctaatgagaa atcagcattt tctttttcgt actctagatg
    23161 acttccttct agaagttgaa cgaattcaat attgtatggg gtattttctt ccacacgttg
    23221 gcgagcgtct acatcgcttt ttgcctcaat gtaaagagat tttgtttctt cccgtttagg
    23281 gttgcgtact tttgtttctt gataataaac tttatagatc attgttttgt ctcctttatc
    23341 tgcgtaaagc agtgatttag ctagttgatt tttttagaca acacaaaacg aggggacaag
    23401 ccacctggtt tttcatatgc tttgactaga atactggatt cgagtcaaag agacaagcct
    23461 cgtttttgct tatctgttcg gatatttttt caaccgtacc ttctgtataa aaaatataca
    23521 acaattaatt gttattctag cataaaacag caaataagta tagaaatcaa ctagattgaa
    23581 agaaaaaatc aatgtttatc atcccgctat aatcataatg acttttttat gagagaagtg
    23641 ttctattaat aggctcgttg tgctatcatt tttagtaatg aaaatgtaac gaaagattaa
    23701 aaggagatga gaaagtgaaa ctaatgtggc attataccat gcgttataag aaattcttgt
    23761 ttttcaattt catatgcgta tttggtttta ttttgattga attaggactt cctacgattt
    23821 tagcacgtat gatcgatgta ggaatccgca atgatgattt tgattatgtg aagcaacaag
    23881 ggatatttat gatcgttatt actgtaatcg gtattatttt gaatatcttg ttaggttatt
    23941 ttggttcgag gattacgact aatatcgtag cagatattcg agatgatttg ttcgaaaaga
    24001 tccaaagtta ttcgcatcag gaatacgaaa atctaggcgt atcttcattg atcactcgta
    24061 caacaaacga tgcttatcag atcatgttgt tcctgcaaaa tattttacgt atcggtttta
    24121 tggcaccaat gatgttcatt gtcagtttgt acatggtcat gcgtacaagt gtgacactca
    24181 gtttgtatgt agtaggtgca ttacctttac tattattggc agtcgtagcg attgctaagg
    24241 tgtctgaacc gttatctaaa aagcaacaaa aaaacttaga taaaatcaac agtatcttgc
    24301 gggaaaatct ttcaggattg cgtgtgattc gtgcattcgt caatgaaaag tttgaagaaa
    24361 aacgttttgc tgtagtcaat gagaattatg cttctagttc aaaaagctta ttccgtttga
    24421 tggctgttgc acaaccgggt ttttatttct tatttaatat cgtaatggta ttgatcatct
    24481 ggagcggggc agtgcaaatc gatgcaggcg agcttcgtgt aggagatttg attgcgttta
    24541 tcgagtatat attccatgcc ttgttctcat tcatgttatt tgctagtgtg ttcatgatgt
    24601 atccaagagc agctgtttca gctggacgta tccaagaagc cctagatgcc aaaccttcga
    24661 ttgtagaaga tccaaatggt atatctgata caaaaactaa agggtatatc gaatttcaaa
    24721 atgtaacatt tgcttatcca ggccattcgc aagagccagt gatacgaaat gtcagtttta
    24781 cagcctctcc aggagagacc gttgctttta ttggaagtac cggtagcgga aaatctacgt
    24841 tgatccaatt gatccctcgc ttttatgatg tgagtgaagg aaagatcttg atcgatggag
    24901 tagacgtgag agattatcgg ctaagcaagt tgcgagataa gattgggttt attccgcaaa
    24961 aagcgctgct atttactgga acgattgcag ataatttgcg atacgggaaa gaagatgcta
    25021 cgcaagaaga aatggaacgt gcagctgaga ttgctcaagc ttcagaattc atttctcgaa
    25081 aaccagatgg atttaatgaa catttatcag aaggtggttc caatttttca ggtggacaaa
    25141 aacaacgatt ggcaatagcc agagccatca tacgccgtcc ggaaatctat atttttgatg
    25201 acagcttttc tgcattagat tatcaaacgg atgcgaagtt acgtgctcgt ctgaaaaaag
    25261 aaacgacaga atctactgta ttgatcgtag cacaacgtgt gggaacgatc atgcatgcag
    25321 ataagatcat cgtattaaat gaaggagaag tcgtcggtat ggggacgcat cgggagttgt
    25381 tggaaaaatg cccgatttat tatgatattg ccgcttctca attatcaaag gaggaattag
    25441 catgaaaaaa accttttctt ccttaaaacg tttagctcga tatatcagac cgtatcgtga
    25501 gacttttctt ttagtgatcc tatttacagt tctgacagtg gctttcaacg tggctttgcc
    25561 atatgttaca ggacttccta ctacagaaat cagccgtaat attgccaatg gcgaatcaat
    25621 caatttttct tatatattca aatgtctcat ttggatcaca gttgttggaa tagggtattg
    25681 tatttcgcaa ttactttctg gtgttttgat gacaaatgtc gttcaaagtg ccatgcatga
    25741 tttgagaaga gacatagaag aaaaaatcaa tcgtcttccg gtttcatatt tcgataaaaa
    25801 ccagcaagga aatattttgt ctcgtgtgac gaatgatgta gatgcagtca gcggtgcttt
    25861 acaacaagct tttattggta tcgtaaatgc gatattggga attacgttgg ctgcagcaat
    25921 gatgttttat atccagccga tgatggcgct gatctctatg atcatgattc caacatcgat
    25981 ctggatctca aaacgagtga tcaatgcttc gcagaaatat tttcaaagca tgcagaattc
    26041 tcttggtgag ctaaacggct acgtccaaga aaatatgaca ggttttagtg tattgaaagt
    26101 atatggacgc gaaaaagaaa cattggaagg attcaaaaaa gttaatcaca gcttgaaata
    26161 ctttgggttt cgtgcagcat tcatttctgg cttgatgatg cctctagttc aattaactgc
    26221 ctatgctact tatattggta tggctgttct aggaagcttt tatgttatta ctggggcgat
    26281 cgttgtcggt caactacaag cattcatcca atatatttgg caaatcagtc agccaatggg
    26341 gaacgtgaca caattgtctt cagttttgca aagtgcatca gcggcaacga aacgtgtatt
    26401 cgagatttta gatgagccag aagaaaaaga aaacaaagta gatgcttcac tgccagaaac
    26461 gattttagga gatgtgacat tcgatcatgt cgattttgca tatgatccga agaaaccatt
    26521 aatccaagat ttgaattttg aagtgaaagc tggtcaaacc gtagctgtgg tcggtccgac
    26581 cggtgccggc aagacgacat tgatcaatct attgatgcgt ttttatgacg tagacaaagg
    26641 agcaataagg attgatggaa tcgacaccaa atcaatgagt cgcagtgatg tgcgttcgct
    26701 atttggtatg gtcttgcaag atgcatggct ttatcaagga acgattgcag ataatatccg
    26761 cttcggaaag cttgatgcga cagattatga agtagttgat gctgcgaaaa cagcaaacgt
    26821 agatcatttt atccgaacga tgccagatgg atatgatatg gccatcaatt ctgagggaga
    26881 caatgtgtct ttaggacaaa aacagttgct gacgattgcc cgagcagtgg tctccgatcc
    26941 taaaatcttg attttagatg aagcaacaag ttcagtagac acccgtttag aagcgttgat
    27001 tcaaaaagca atggatcgcg tgatggaggg acggacaagt ttcgtcatcg ctcaccgttt
    27061 atcgacaatc cgtgatgcag atctgattct agtgatggat caaggtcaga tcattgaaaa
    27121 aggaacgcat gagagtttat tggcacaagg tggattttat gaaaaactgt ataacagcca
    27181 atttgctgag gaagcagaat aagtcatata agaataaaac agtttaaaac ttattttttg
    27241 ttctctattc ttgataatgg taaagtaaag ttatcaagaa aagtgaggac acggagatgg
    27301 aacagttttt aaatgaaaaa attactgaag ccaaaagaga agttgatcgt ttaacgcacg
    27361 tctgtcagac gaagcctttt gattcagctg atttttttga aaatgagttt caaatcgaac
    27421 gcttggcagc acaaatagaa gtgtacgaat taattttaaa aaaagtaata gaagaaaata
    27481 tagatgtaca ataaaaacag gtaaaacgtc agttaccaga tactgtaaat agacatctgg
    27541 cctgagcagg ttttacctgt tttttttctt atgaaataaa tatatgctat gataccttta
    27601 ttcaatagga ttagtttcta caaaaaatga aaggagaaaa ataaatatgg aacggtttat
    27661 agcatcaaat gccacagtga ttggtgatgt gacattgtca gaagatgtga cgatctggta
    27721 tcaagctgtt ttacgcgggg acagtaattg gataaaaata ggacaaagaa cgaatatcca
    27781 agatggtaca atcatccatg tcgatcatga tgcacctgta gatattgcgg agaatgtgac
    27841 ggtaggacat cagtgcatgc tacatggctg tacaatcgaa aaaggggcgc tgatcgggat
    27901 ggggaccacg atattgaatc atgcggttat tggtgagaat agtttgattg gtgctggatc
    27961 attagtaaca gaaggaaaag ttattcctcc taatgtcttg gcttttggtc gcccagctaa
    28021 agtgatccgt cctttgacaa aagaagaaat acaaaaaaac aaagaaaaca tccagcatta
    28081 tgtcgagata ggtcggatgc atgcgcaagg aaaattcaag gagcaaagat aggcagtagc
    28141 atccataaaa taaaaaagga tggtcagttt gagttgacca tcctttttta tttggtttag
    28201 ataagagagc ttaaaaaagc ttcctttgaa acagattaat ttaatgaaga actgttttcc
    28261 atgacttcat cgatcaatcc atatgctttt gcttgttcag ctgtcatata gttgtcacga
    28321 tctgtatctt tttcaatcac ttcaagcggt tgtccagtgc gttcagctaa aattttgttt
    28381 aagcgttcgc gtgtttgcaa aatatggcgt gccgcaattt cgatttcagt tgcttgccct
    28441 tgagcgccgc caagaggttg atggatcatg atctcagcat ttggtaaagc aaatcgttta
    28501 ccttttgtac cagctgtcag caagaagctt cccattgaag cagccatacc cataacaatt
    28561 gtttggacat ctgctttgac aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact
    28621 gaaccaccag gagaattgat ataaagatag atatcttttt cagaatcttg agcgtctaag
    28681 aaaagcaatt gggcgatgat cgaattagca agatcatctg ttacttggcc gcttaacatg
    28741 atgatacggt ctttcaataa acgtgagtag atatcgtaag ctctttcacc tcgtgaagat
    28801 tgttcaatga ctgtaggaat taaattcata atagagttcc tccttgattt cgtttagaaa
    28861 tagtgtacca taatttagtt tctaatacct aaaatgtagt atacattttt ggtcaaaaaa
    28921 ggtcaaataa aaaacttagg caaattagtc gcataaaccc gctttatggt atgataagca
    28981 gagaaacaaa acaaggagga acaacagatt gatagttggt attactggcc ttatcggagt
    29041 gctgacaggt gctgtagttg tctatatcgg catgatactg caaatgcgtc acttggaaaa
    29101 taaagaacag cgtagacgtg aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca
    29161 attaaataaa aagatcaacg agatcttgca aaaacggaca acgctgatgg aagaatatgt
    29221 cagctttgat gcatttgatg attgttatat cacgattgac gattttgcgt atctccaatc
    29281 attttcagcg caaaatagtt tttatctgcc aaattatatt ttagacgagc tatttaagaa
    29341 tatctcccat cgaaaagtga ttttatctcc agatgaaaca atgaagattg gtggatatgc
    29401 ttataaaggc ggacgcgtga ttttagagaa ttttacggat agtctgactg aaatgatcaa
    29461 tgaaaaaaaa gctcagttga agactatgac gcgccagccg attggtttct tctcgaaaga
    29521 tgatgtttgg agctgatatt ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt
    29581 tctttttttc ttaatgatac aatagaactg aacggagtga agaaagatgt tacaaatcta
    29641 tgcccatcgc ggaagcagcg gcacccaccc agaaaatacg cttcctgcat ttgcagaagc
    29701 tgtacgagta ggtgcagatg gtatcgaact agatgtccat ctaagcaaag atggttattt
    29761 gatcgttatg cacgacgaag aagtggaccg gacgactaac ggtaaagggc tgattcgaga
    29821 aaaaacattg gaagaactca aaaaattgaa tgcagggggc aaattcagca aagaatttcc
    29881 agcagctaaa gttcctgaat tgagagaagt gttaggactg ctcctccaaa aaaattaccg
    29941 agggatgcta atgattgaaa ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa
    30001 attagccgaa acattaaaaa gtagagattg gccttttcaa catgcatatt gcagctttca
    30061 tttggattct ttggaaaaaa tcgcattact ggaaccggag atacagctgg acttattgat
    30121 gagtacttct gataaaaaga tccaactagc agcagaaacc ccttatatag aaggagtaca
    30181 tccaaaaaat acttggttgt tcgaacatcc agtccaggca gctgattttc caaaagcaat
    30241 tcgtgcatgg acgattaacg atgaagaaga aatggccaaa tgtcaagaac tcaaaataga
    30301 tggaataata actgattttc cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag
    30361 gtaaaatatg gaaaaagtat tagtgatcgt cggtccgaca gcagtcggta aaacagcgct
    30421 aagtgtggaa ctcgcacaac tatttggagg agagatcatc agtggtgatt cattgcaaat
    30481 ctataaaaat ctggatatcg gtacagcaaa aatcaaagaa gaagagatga ataatgttcc
    30541 gcatcatttg attgatgtga tcgaacctac tcaacgttac tcagccgctg attttcagca
    30601 ttcaggtcga caattgatta ccgagattgc gaaccgaggg cacctaccta tcatagctgg
    30661 tggtacgggt ctctatatcc aatcccttct atatgattat cagttaggag cgacagaaga
    30721 agcgaatcct gagattagaa aaaagtatga aacgttagct gaaacaattg gttcccaagc
    30781 attatggaat cagttgaaag aaaaagaccc ggcagctgct gagaaaatcc actggaacaa
    30841 tcaacgcaaa gtgataagag ctctggaagt tttagagacg acaggacata gtatcacggc
    30901 acctaaagaa gagccaaagc aattgtatga ttacctcatg attggattag atacgaagcg
    30961 tagtattctt tatgaacgaa tcaatcaaag agtcgatttg atgctagcag aaggtttagt
    31021 agcagaagcg cgtaaggtgt atgagcttgg agaagtccag gccagtcaag gtattggata
    31081 taaagaattt ttcccttatt ttgaagggaa agaaacattg gaagaagcaa cagaacaagt
    31141 caaactacat tcgagaagat acgcgaaacg tcagttgacg tggtttcgta accgtttgga
    31201 tgcacagtgg tttgatctta taagtgagcc aaatcaaaaa agtcagattg agcaaacaat
    31261 caaaaaatgg ctggaggcgg aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa
    31321 ccgaagcaaa ccagcgttac ttttcagagt caatggaaga gctagttcag ctgacgaata
    31381 ccgcatcagg agaagtcgta ttcacaatta cacaaaagag accacaagtg gatcggcaaa
    31441 cgattatcgg taaagggaaa ttacaagaat tagttcaaca agcggatgcg catgaagcgg
    31501 atttgatcat ctttaatcat gagctaactc cgagacagag ccaactgatt accgatgctg
    31561 ttggtcttcc ggtaattgat cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc
    31621 gttcgaaaga agggaagctg caggttgaac ttgctcagtt agaatacctg ctgcctcgtt
    31681 tagtagggca agggaaaaca ttatctcgtc ttggcggtgg gatcggaaca agagggccag
    31741 gtgaaacaaa gttagaaaca gaccgccgac atatacggaa taaaattttt gctgttaaga
    31801 aagaattgaa agaagtagaa gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg
    31861 agatttttca gattggactt attggctaca cgaatgccgg gaagtcaact atcttgaatc
    31921 tattgacaca agcggatact tattcgaaag atcaattgtt tgcaacgtta gatccattga
    31981 ctaagagatg gcgttttgct gaaggatttg agattactgt aacggatacc gtcgggttca
    32041 tccaagacct gcctacgcaa ctgatcgatg ctttccattc tacattagaa gaaagtcaaa
    32101 atatggatct tttgctacat gtagtcgatg ctagttctcc agatcgtatt ttacaggagc
    32161 agactgtctt aaaattgatg gatgaactga atatgaaaga aatgccgata ctgactgttt
    32221 acaataaggc agaccagatc gacccagcga tgttcacgcc tacgctattc ccaaatgtat
    32281 tgatttctac tcagacgaaa gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc
    32341 tgatggaact aatgacccct tatacacttt ctgtgccaag caacgaagga cagaaattaa
    32401 gcgagttgag aagacaaaca atggttttgg acgaagcgta catggaagaa acaaacgaat
    32461 atgaagttaa agggtttgca aaaaccaatt caaaatggtt aagaaaaaac caataaaatt
    32521 ttattttttt aa
//
LOCUS       DACDNO010000029        31643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDNO010000029 DACDNO010000000
VERSION     DACDNO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.029"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(135..512)
                     /locus_tag="IWI22_001424"
     CDS             complement(135..512)
                     /locus_tag="IWI22_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168822.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            768..1496
                     /locus_tag="IWI22_001425"
     CDS             768..1496
                     /locus_tag="IWI22_001425"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9168823.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1496..1750
                     /gene="purS"
                     /locus_tag="IWI22_001426"
     CDS             1496..1750
                     /gene="purS"
                     /locus_tag="IWI22_001426"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9168824.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1752..2423
                     /gene="purQ"
                     /locus_tag="IWI22_001427"
     CDS             1752..2423
                     /gene="purQ"
                     /locus_tag="IWI22_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9168825.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2424..4646
                     /gene="purL"
                     /locus_tag="IWI22_001428"
     CDS             2424..4646
                     /gene="purL"
                     /locus_tag="IWI22_001428"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9168826.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4631..6070
                     /locus_tag="IWI22_001429"
     CDS             4631..6070
                     /locus_tag="IWI22_001429"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9168827.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6102..7145
                     /locus_tag="IWI22_001430"
     CDS             6102..7145
                     /locus_tag="IWI22_001430"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9168828.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7142..7720
                     /gene="purN"
                     /locus_tag="IWI22_001431"
     CDS             7142..7720
                     /gene="purN"
                     /locus_tag="IWI22_001431"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9168829.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7717..9252
                     /gene="purH"
                     /locus_tag="IWI22_001432"
     CDS             7717..9252
                     /gene="purH"
                     /locus_tag="IWI22_001432"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9168830.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9268..10542
                     /gene="purD"
                     /locus_tag="IWI22_001433"
     CDS             9268..10542
                     /gene="purD"
                     /locus_tag="IWI22_001433"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9168831.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10664..11578
                     /locus_tag="IWI22_001434"
     CDS             10664..11578
                     /locus_tag="IWI22_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9168832.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11711..13276)
                     /locus_tag="IWI22_001435"
     CDS             complement(11711..13276)
                     /locus_tag="IWI22_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9168833.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13269..14720)
                     /locus_tag="IWI22_001436"
     CDS             complement(13269..14720)
                     /locus_tag="IWI22_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9168834.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14734..15750)
                     /locus_tag="IWI22_001437"
     CDS             complement(14734..15750)
                     /locus_tag="IWI22_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9168835.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15970..17058
                     /locus_tag="IWI22_001438"
     CDS             15970..17058
                     /locus_tag="IWI22_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9168836.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17112..18638
                     /locus_tag="IWI22_001439"
     CDS             17112..18638
                     /locus_tag="IWI22_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9168837.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18640..19845
                     /locus_tag="IWI22_001440"
     CDS             18640..19845
                     /locus_tag="IWI22_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9168838.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20102..21079
                     /gene="guaC"
                     /locus_tag="IWI22_001441"
     CDS             20102..21079
                     /gene="guaC"
                     /locus_tag="IWI22_001441"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9168839.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21343..22767
                     /locus_tag="IWI22_001442"
     CDS             21343..22767
                     /locus_tag="IWI22_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9168840.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23228..24337
                     /gene="pdhA"
                     /locus_tag="IWI22_001443"
     CDS             23228..24337
                     /gene="pdhA"
                     /locus_tag="IWI22_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9168841.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24340..25317
                     /locus_tag="IWI22_001444"
     CDS             24340..25317
                     /locus_tag="IWI22_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9168842.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25342..26985
                     /locus_tag="IWI22_001445"
     CDS             25342..26985
                     /locus_tag="IWI22_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9168843.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26993..28399
                     /gene="lpdA"
                     /locus_tag="IWI22_001446"
     CDS             26993..28399
                     /gene="lpdA"
                     /locus_tag="IWI22_001446"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9168844.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28561..28836
                     /locus_tag="IWI22_001447"
     CDS             28561..28836
                     /locus_tag="IWI22_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9168845.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28842..29609
                     /locus_tag="IWI22_001448"
     CDS             28842..29609
                     /locus_tag="IWI22_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9168846.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29777..31606
                     /gene="typA"
                     /locus_tag="IWI22_001449"
     CDS             29777..31606
                     /gene="typA"
                     /locus_tag="IWI22_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9168847.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT        10357 a         5434 c         7117 g         8735 t
ORIGIN      
        1 aaccaaaaaa gaagcagatc cgttagcggt tctgcttctt ttagtttatt atattttctt
       61 atcaataata ggaacctgat tttaaaagcg gctacctctt taccgtgcct ctttacatac
      121 aaaaaataga ggaattaagc gtaaatggct tggctattat aactaaaatg ctggttatgg
      181 agatgttgca gcaagttttc gagaaaagcg tattccatct cttgtccatt gaataagatc
      241 tggatacgat aatgattttt gaaacgcttc tgttcatgaa tctgactgat tgaaagttcg
      301 cctttgtgtt ctgtctgata aagaaaagtc cttttacggc attcagcaca gcgcttggtt
      361 gctcggcgga tttttcggaa tgtataaaaa ctagtatctt tattgcataa ataaacgtct
      421 ttgatcgaca gcaaatcatt atatagacag gaacgttctt cagctgttaa ttctgataaa
      481 aaaagctgac cttgttttat tgcatcttcc atcggcaagt gcctcctcga acccattatc
      541 caaattttta gttaattcta atataccatc attaagtata aatgtaaacg cttgttgatg
      601 gtataagatt ttcgtttctg ataaaacacg aacattaaaa gaatctaatg ccttaaatgt
      661 acatattttt gttgatttat aaaaagatga ttgctaaaat ttaaagtgaa aaaacgaaca
      721 aaaaacgatg tatttgcttt taatgtaaat aaaaaggaga gacgtacatg gagaagaaag
      781 aacttttgta tgaaggaaaa gccaaaaaaa tatatcttac ttctgatgag aacttgattt
      841 ggatagaata tttagatcaa gcaactgcgt tgaatggcgc gaggaaagac cagataagtg
      901 gaaaaggaga attgaataat caaatcacaa gtgcaatttt ccgttatctg actcaaagag
      961 gaataaaaaa tcattttatt gaacaattgt ctaaaaccga acaattagca aaaaaagtac
     1021 aaattatccc tctggaagtg gttatccgaa atattgcagc aggaagtttc tcaaaacgtc
     1081 taggtgttcc cgaaggtaaa gaattcatca aaccaatcat tgaattctac tataaggatg
     1141 accagttgga tgatccattt atcaatgaag agcatatcta tttcttacaa cttgcaaagc
     1201 cggaagaaat cagtgagctg aaagcccaag cgttggctat taatgaaatt ttgaccgaat
     1261 tatttgctaa ggtcggtttg gttttagtag atttcaagct agaatttggc cggtcgaatg
     1321 gacaaatttt gcttgcggat gaagtaacac cggatacttg ccgattatgg gatgaacgta
     1381 cgaatgaaca tatggacaaa gacgtttacc gcagagatct aggtgatttg atcccagtct
     1441 atcaagaagt atataaccgt ctatcggaac taactgaaga aaaggagcga gtatgatgta
     1501 tttcgtaaaa gtatatgtaa cctataaaga ttcagtgtta gatcctcaag gggaggcagt
     1561 aaaaaaagca gtccatcgta tgggatatga aacaatcgaa gatatccgaa taggaaaata
     1621 ctttgagata caacttgctg atgcagaaga accaatcgat acagtgattg aaacgatttg
     1681 tgacaagctg cttgcaaatg taaatatgga aacataccga tatgaaatca aaaaaatgga
     1741 ggaagtataa catgaaattt gcggtcatcg tttttccagg ttcaaattgt gacgcagata
     1801 tgttatgggc agttcgcgag atcatgggcg ctgatgccga atttgtacgg catgatgagt
     1861 cctctttaga aggatttgat ggggttttac tgccgggagg attctcctat ggtgactatt
     1921 tgagatgcgg tgcgattgct cggttttcta caatcatgga agaagtgatt cgttttgctg
     1981 aagaagggaa accagtattc ggtacatgca atggattcca gatattgaca gaagcaggac
     2041 ttttgccagg tgtactgaga cgaaatgagt ctttgcattt tatttgcaaa actgtaccat
     2101 tgaaagtcaa ccccaagacg gcctttactt cagagtatga agccgacgaa ataatctatc
     2161 ttccagttgc tcatggggaa ggaaattatt tctgtgacga agaaacactt caagaactaa
     2221 aaaaaaataa tcagattgcc tttacttata atgacaacat caacggcagc gtggaaaaca
     2281 ttgcagggat tacgaacaaa gcagggaatg tgttaggaat gatgcctcat ccagaacgtg
     2341 cagtagaaga cttgctagga tcagaagatg ggaaacggtt ctttgcttcc attttaaaaa
     2401 acttcggaaa ggtgaccgta tagatgaaaa cattgaaaga accgacagct gaagagatca
     2461 agaaaaaaag aatctatgcc gaatggggat tgactgatga agaatatcga atgatcgaag
     2521 aagatatctt aggaagaatg cctaattata cggaaactgg actgttttct gttatgtgga
     2581 gcgaacattg ttcctataaa aattctaaac cggttctacg caaatttcct actagtggac
     2641 ctcatgtgtt gcaaggccct ggagaaggcg caggcatcgt cgacatcggt gatggactag
     2701 cagttgtctt caaagcagaa agtcacaatc atccttcagc cgtggagcct tatgaaggag
     2761 ctgcgacagg agttggcgga atcatcagag atattttcag catgggggct cgtccaattg
     2821 cgttgctaga ttcattgagg ttcggcgaat tagataatcc aagaacgaaa tatttactag
     2881 aagaagtagt tgcaggtatc ggcggatacg gcaattgtat cggcataccg actgtaggcg
     2941 gcgaaattgc atttgatcct tgttatgaag gaaacccatt agtcaatgca atgtgcgtag
     3001 gtttgattga acacaaacat atccagaaag ggcaagccgc tggcgtcggt aattcgatca
     3061 tgtatgtagg tgcaaaaaca ggtcgcgacg gtatccacgg agcaactttt gcctcagaag
     3121 aatttaatca agaagaagaa cagcaacgtt ctgctgtaca agtaggcgat ccttttatgg
     3181 aaaagctgct gcttgaagcg tgtttggaat taatcttgca tcattcggat tctctaatag
     3241 gtatacagga tatgggagca gcaggacttg tttcatctag tgcggaaatg gcttcaaaag
     3301 ctggctcagg acttatattg acattagatg aagtcccaca aagagaaact gggatgacac
     3361 cttatgaaat gatgttatcg gaatcacaag aacggatgtt gatttgtgtt aaatcaggag
     3421 aggaagaaaa aatccaagaa ctatttcaga agtatgatct agatgcagtc actatcggta
     3481 aagtaacaga cgacgggcaa tatcggctat atcatcaagg agaagaagta gcaaatctgc
     3541 cagtcgatgc attagctgaa gatgcaccag tttatcataa agaaatgaaa gaaccagctc
     3601 gaatcgtaga atttcagcag ttagctcctt atcagccagt tatcgaagaa cctggacaag
     3661 tattgcttga tctgctgcag caaccgacga ttgcttcaaa acgttcaatc tacgaaactt
     3721 atgattcaca agtacaaacc aatacagttg tccgtccagg aagcgatgct gctgtgatga
     3781 gggttagagg gacgaataaa gcgttagcta tgaccacaga ctgcaacgcg cgctatctgt
     3841 atttggatcc aaaaatcgga ggacaaatag cagtggcaga agctgctaga aatattattg
     3901 ctagcggtgg taagcccttg gcgatcaccg actgcctgaa ctatggttct cctgataaac
     3961 cagaagtttt ttgggaatta tcgacttcag ctgacggaat tgctgctgcg tgtgaaacgt
     4021 taggaactcc tgttatttca ggaaatgtct ctttatataa tgaaacggat ggtcaagcaa
     4081 tctatccaac accaatgatt ggtatggtcg gattgattga ggatcataaa catatcacta
     4141 ctcaggaatt caaaaaatca ggggatctaa tctatatatt aggtaagacg tttgcagatt
     4201 ttgatggcag tgaattacaa aagatgcagc ttggaagaat cgaaggtgtg atccgaaact
     4261 ttgatctatc cattgaaaag agaaaccaag agttagtatt gacagccatc caaaacggat
     4321 tgattgaaag tgctcatgac tgttcagaag gtggcttggc aattgcactg gcagaatcag
     4381 ctttcaaaca tcagctaggg atatctgtac agtttgaatt gtcctctgct caattatttg
     4441 cagaaactca atcaagattt gtgttgacag tcgcaccgga aaataagaca cgattcgaag
     4501 aaatgatggg agatgctgct gttttagcag gtaaggtgac ggatgaagcg ataatcgaaa
     4561 tttcagcaac tgacggtcag atcaaaatag aaactgcagt agcaagaaag tgctgggagg
     4621 atgcaatcgt atgtcttatg aagtgaagag tttaaatgaa gaatgcggga tcttcggtgt
     4681 ttggggtcat ccagacgccg ctagtgtggc atacttcggg ctccatagtc ttcagcatcg
     4741 aggacaagaa ggtgcaggta tcgtgtccaa tgatcgagga aagcttaagg ggcatcgaga
     4801 tttggggctg ctttctgagg tattcaaaga tcaaagaaaa ttgcagcagt taacaggaga
     4861 agctgcaatc ggacatgtac gttatgcaac agcaggcaat ggaagtgtgg acaatattca
     4921 gccttttcta ttcaaatttc atgatcagtc gatcggctta gcgcataatg gtaacttgat
     4981 caatgctcgt tcccttagaa aagagttaga ggaaaatggg gcgatcttcc attctaactc
     5041 cgatacggag atattgatgc atttgatccg acgaagtgaa gaaacagatt ttttaaataa
     5101 attaaaaagt gctttgaatg aagtcaaagg tggctttgcc tatttattaa tgacagaaac
     5161 ggcaatgatt gccgctttgg acccaaatgg ttttcgtcca ttagcaatcg gacaaatgaa
     5221 aaacggagca tacgtgattg cctcggaaac ttgtgcatta gaagttattg gggcacgttt
     5281 tataagagat gtccagcctg gtgagatagt gatcatcaat gacgaaggaa tccagattga
     5341 ttgctttacg aaggacacgc agctttctat ttgttcgatg gaatatatct attttgccag
     5401 accagattca aacatcgcag ggatcaatgt gcatactgca cggaaaaata tgggacggaa
     5461 tttagcgaaa gaatcgccag taaaagcaga tatggtcgtt ggcgtaccta attcttcttt
     5521 gtcagccgca agcggttatg cggaagctag tggaatccct tatgaaatcg gattagtaaa
     5581 aaatcaatac attgcccgaa catttatcca gccaacgcct gagctaagag agcaaggtgt
     5641 tcgaatgaaa ttatctgctg tacgaggagt agtggaaggt aaaaaggtga ttatggtcga
     5701 tgattcaatt gttcggggta cgacaagcag acggatcgtc catcttttga aagaagcagg
     5761 ggcaaaagaa gtccatgtga ggattgcttc tcctcctttg aaatatccat gtttttacgg
     5821 gattgatatt caaacgcgta aagaactcat tgcggcaaat cacacgattg atgaaatcaa
     5881 agaatgtatc ggtgcagatt ctttaggttt tttaagtgaa gagggtctga tcgaatcgat
     5941 cggtctggaa aaagatgctc cgtattcagg attgtgtatg gcttatttca acggagatta
     6001 tccgacgccg ttatatgact atgaagaaaa atatcaggaa tcactaaaag aaaaagtatc
     6061 gttcttttaa aagcagacca agaagggagt atatgaattc aatggcaaat gcatacgcaa
     6121 aagctggcgt agatgtcgaa gctggatatg aagtagtaga acggatcaag aagcatgtca
     6181 aaaaaacgga acgtttaggt atgatgggcg ctataggagg attcggcggt tgttttgatt
     6241 tatcggtctt tcaattaaaa gagccagtcc ttgtctcggg gaccgatggc gtggggacga
     6301 aattagcagt ggcaatcgaa gcagacaaac acgatacaat cgggatcgac tgcgtagcga
     6361 tgtgtgtcaa tgatatctta gcacaaggcg cagagccgtt atatttctta gactacttag
     6421 caacggggaa aaatgatccg ttacgtttgg aaaatgtagt aaaaggtgta gcagatggat
     6481 gtgttcaagg aaatatggcg ctgattggcg gagaaacagc tgaaatgcct ggtatgtact
     6541 caggtaatga ttatgatcta gccggatttg ctgtagggat agctgaaaaa tctcaattat
     6601 tgacaggaga aacgatcaac ccaggagatg tgttgcttgg aatagcatct agcgggattc
     6661 attcaaatgg gtactctctt gttcgcaaaa ttttctttga gcagcagcag ttttctttat
     6721 catcaaaact gccagaattg aaaaaaacac ttggcgaaga attgcttgag ccaacgaaaa
     6781 tctatgtttc ttccttgctt ccgctgatca aagaaaagaa aataaaagga gctgcacata
     6841 tcactggtgg agggtttgta gaaaatatcc cgcgaatgtt accagaatct atggctgcac
     6901 aaatcaaact tggctcatgg ccgattcttc caatattcgg ctgtttacaa aaatatggtg
     6961 aaattcctga gatggagatg tatgaaatct tcaatatggg catcggtatg gtcctggcag
     7021 tcgaagaatc gaaagtaaaa gaaatagtac acgaaattga gcaagctggc gaaaaagttt
     7081 atgaaatcgg gaaagtgatc gccaaagaaa aacatcgtgt ggtgtttata ggagaagaac
     7141 gatgagaatc gctgtttttg cttctggaaa cggaagcaac tttcaagcgc tagccgatta
     7201 tctatcgaaa aaaggattgg aatcttcgat tgactggttg ttttgtgatc agcctgaagc
     7261 atatgttttg aaacgtgcaa ctgcgttgag tgtaccggca gattgttttt cacctaaaga
     7321 atttgattca aaaaaagagt atgaagaagc aattttgcat aaattgaaag aaaaaaagat
     7381 cgatttgatc gtattagctg gatatatgcg aatcattgga ccggtattgc tgaaaaatta
     7441 tgataaaaga atcatcaata tccatccgtc tcttttacct gccttccctg gtttgcatgg
     7501 catacgtgat gcctttgaag ctggagtaaa agaaacgggt gtcacaattc attatatcga
     7561 ccaaggtgta gatactggac caatcatccg gcaggaaaaa gtgagaattg aacaggagga
     7621 cacgttcgat tctttagaag aaaaaatcca tagagtagag catcgaatct atccagaagt
     7681 gattagtgaa attattgaaa atggaggcta tctgaaatga caagagcatt gatcagtgta
     7741 tcagataaaa ctgggattgt agcattcgcc caaggtttga ttgaacaagg agtagaaatc
     7801 atttcaacag gaggaacaaa gcatgcgttg gataaagctg ggatcaaaac gctttcgatc
     7861 gaagaagtga cagggtttcc tgaaatgatg gatggccgtg tcaaaacact tcatcctaaa
     7921 atccatggtg gtcttttagg aagacgcgat ctccctagcc atatggaggc aatggaaacg
     7981 gaaaatatcc aaccaatcga ctttgtttgt gtcaatttat atcctttcaa agaaacgatt
     8041 atgaaaccag atgtcgaaat agcagaagct attgaaaaca ttgatatcgg tggtcctagt
     8101 atgctccgta gtgcagcaaa aaaccatgaa tttgtcacct ctgttgttga tccatctgac
     8161 tatgaagaag tactttcaga attacgtcag tatgggcata cttcactttc tttcagacaa
     8221 aaactagcgg caaaagtctt tcgtcatact gcggcatatg atgcactgat cgctcaatac
     8281 ttaacggatt gggtggaaga aaaagaaccg gaaaaattga cacttacgta tgaacgaaaa
     8341 caaacgcttc gttacggtga aaatagccat caagccgctg ccttttatca gtccgctctt
     8401 ctggaaaaat attcgatcgc agcagcaaaa caactccatg ggaaagaact ttcttataat
     8461 aatatccgag atgcagatgc tgcgctacgg atcatgaagg aatatgacga gccaacggta
     8521 gtcgccttga aacacatgaa tccttgtggt attgggacag catctacgat tttagaagcg
     8581 tgggaagcag cgtatgaagc agatcctgtt tcaatctttg gtgggatcat cgttttgaat
     8641 cgagaagtag atctgccaac agcagaacag atgcataagc tgtttctaga aatcatcatt
     8701 gccccttctt atacacaaga agctttggaa ttactgcaaa aaaagaaaaa catccgatta
     8761 ctgacagtag actttgcgca ggaagaaaat caaaatcgag aagaaacggt ctcagtctta
     8821 ggtggacttc ttgtacaaga tcaagatctg gcaatagaaa atgaagaaga gtggaaagtc
     8881 gtgacaaaac gtaagccgac tccagcagaa gcaaaagcat tagcgtttgc ttggagagct
     8941 gtcaaacatg tgaaatctaa tgcgatcgta ctggcaaacg aacgtcagac agtaggaatc
     9001 ggagccgggc agatgaatcg agtcggttca gtaaaaattg cgattgaaca agcgcaagag
     9061 aaaatggaag gtgcagtttt agctagcgat gcttattttc caatggatga ttcggttgaa
     9121 tatgcagcca aacatggtat aaaagcaatc attcagccag gaggaagcat caaagaccaa
     9181 gattctattg atatggcgaa taaatacgat ctagcaatgg tctttactga tgttcggcat
     9241 tttagacatt agagaggagt cggataaatg aacattttgg tgatcggtgc aggtggtcgt
     9301 gaacatgcaa taagtaaaaa acttttagcc agtccgaagg tggatacggt gtattgtgcg
     9361 ccaggaaatc cggggatgac aaaagatggt atcaattgtg tcgctatcag tgagcgagat
     9421 cattcagcgt taatccattt tgttaagaaa gaggctattg attggagtat cgttggtcct
     9481 gaagttcctt tgttaaatgg gatcgtcgat gattttatgg cggaaggatt gaaaatcttc
     9541 ggtccgacaa aagcagcagc tctgatcgaa ggttcgaaaa gttttgctaa acaattaatg
     9601 gatacttatc aaatcccgac agctaaatcc agaagttttg aatgttacga aaaagcagca
     9661 gcgtatatca aagaaaatgg tgcgccaatc gtagtgaaag cggacggctt agcagctggg
     9721 aaaggtgtag tcgtggctac cacagtgaag gaagcgctgg atgcggcaga acaaatgttg
     9781 aaccagcatc gatttgggga tagtagcaaa aaaatcgtcg tagaagagtt tttggcagga
     9841 gaagaattct cccttttggc gtttgtccgt aataccaagg tgtatccgat ggtcatttcg
     9901 caagatcata agcgggcatt tgataacgat aaagggccaa atacaggcgg aatgggtgct
     9961 tattcaccag taccgcaaat ttcaaaaaag atgattgaaa aagctgtgga agaagtttta
    10021 aaaccagcgg ctttgggaat gaaagaacta ggacgctctt tcacgggtgt tttatatgcg
    10081 ggtttgattg ctacgaagga aggaccaaaa gtgatcgaat tcaatgcgcg tttcggtgat
    10141 cctgaaacgc aagtcgttct ttctcggttg aagagtgact ttgcgcaagt aatcgatgat
    10201 ttattagaga atcgtacagt agagttaaag tggcagcaag aaggatacaa tgtaggcgtt
    10261 gtggttgcag ctgccggtta ccccgaagaa tatgagacag gaatggttct accagatttt
    10321 ctggaagaag agctttctgt ttactatgct ggagtttcct caaataacgg caaactagtt
    10381 ggagcaggag gacgtcttta tttagtagaa gcatacggtg agacgatcaa agaagcacag
    10441 aaaaaaattt acaccgcgtt aatggataaa acgacagatg gaacatttta tcgtacagat
    10501 attggagaaa aagctgccaa tagcgaactg atcgtgaagt aaatcagctg cttccattta
    10561 taaaaaatac aacaaacata atcggaagcg ccgattttag tgaagccttt tcataaagag
    10621 tgtggtacac ttttgttgtg tatataaata aggaggagtt cagatgaaaa aaattcaatt
    10681 aggtacttct gatcagaaag cagctgcagt gatcttaggc tgtatgcgta tcaacaatgc
    10741 ggaagatcca gtaaaagtga ttgagacagc ttatgaaaac ggcattgatt tttttgacca
    10801 tgcagatatt tatggaaacg gtgaatgtga agaaatcttt gcagatgctc tagcaaaaac
    10861 atcaatcaaa cgagaagatc tttttattca aacaaaatgt ggaatcgttc caggtgtgat
    10921 gtttgatttt tctaaaaaac atatcattga atctgtagaa aaaagtttga cccgtctgaa
    10981 aatggattat gtggatgctt tacttcttca tcgtccagat acattggtag aaccagagga
    11041 agtagctgaa gcgtttgatg aattggaacg gtcaggaaaa gttcgttatt ttggcgtcag
    11101 caatcaaaaa ccaatgcaaa tcgaattgct gaaaaaatat gttcgtcaac cattagtagc
    11161 taatcagctg caatttggct tgaaacatac tggaatgatc gatcaaggga ttcatgtcaa
    11221 tatgacagat gaagcaagta tcgatcatga cggtagtgta cttgattatt cccgattgca
    11281 tgatatgacg atccaagcat ggtcaccata ccagtatgga ttttttgaag gtgtatttat
    11341 cgggaacgaa aaattccctg aattaaacca aacattagaa gaactagcag ctaaatatca
    11401 aacgacacca actggcttgg cttctgcttg gattttacgc catccagcca atatgcaagt
    11461 gatcgccggt acgatgaacg tagggcgaat caaggaaatc gctcaagctt cagaaattaa
    11521 tatgtctcgg gaagattggt acgaattgta ccgtgctgct ggaaacattt taccttaaag
    11581 attgtcataa gcaggtcaaa agggctataa attaaaaaga cagaataact tgaaagctgt
    11641 tttcagcttc gagatattct gtcttttttt attgcaaatg gaaagagtat cattgtacgg
    11701 atttcgttta ttagttattt ttgtttttaa ggaaaagctg tgtccgatat tcttttggag
    11761 aaatcccttg ttttttctta aataaataac tgaaatagtt agggtcatta taaccaattt
    11821 caaaagcaat atctgaaata cgatagtctg attgctgcaa taattttttt gctaattcga
    11881 ttctttgttc tgtcagataa tcgatgaatg tttggttaaa cgattgagaa aaaatcgtac
    11941 tgaaatgaga gggacttaat gcaacttgtt gagcgaccca agttaaggac atatctggat
    12001 cggtaaaatt ttctttgata aaatccaatg catggttcaa aattggctga tatttgttcc
    12061 ttgaaggctg tgagggcaga gcagttaaga aagaaatgaa catataaact gtcgaacggt
    12121 aaagcgagtc gtcggcgctg attgctaagc gttgactggt ggatgtgcaa tgaaccattt
    12181 cttgcgggat tttcttttct ttcaggcaga catgctggag tagcttagtc aagacaaata
    12241 aacgatataa tcgtgttcga gttggtgtgc cttgtggttt cgtcaagtgt tcgatgtact
    12301 tagatacttc gtttttcgat aatgaagaca aatcaagtga gaggtcaaag taaaagacag
    12361 aatcaactgg aagtttcgct tcttgaatca aatcctcata gctgataatt tgttctttaa
    12421 gggaactggc ggtttcaagt atcatctgcg tgatctcaaa agatgcaggt aattcgctga
    12481 ttctttctgc gacaggacca atggcgacag aaaaattatt tttttgtccc ttttctaatt
    12541 catggaccaa cgtttgggct acctgataac tggtctcgat cacattttct tcaagagaat
    12601 caactattaa aaatttgatg aatcgagagg aaacagcaga gaagatcact tgttgattct
    12661 ccctaaataa aaacttcaaa taatcactga gatgagcgta atcttcaaag tctgtactga
    12721 cttggtttgc tgctaataat acggtcattt ttttccctgc taaagagcga ttgaaagatt
    12781 cagcaagttt caatgtttca ctgatcgtaa gtgagccttt gaacaaaccg ttcaagaaat
    12841 gatttttttt caattcgaaa acaagatcag aagaagtgtg cgtttctttt tgctgcaaat
    12901 ctaaagcagt tactgctttt tctattgctt gtttcaattc gctactttta atcggtttaa
    12961 gcagataatc atctgcctgt acctggattg ctcctttcat ataatcgaaa tcgtcgaatc
    13021 cactaataaa tatgagacga gtccaaggaa gtagtttcct tgcttctttt gaaaaagtca
    13081 acccgtccat gaaaggcatt tggatatcag tcagaatgat gtcgggctgt aagtcgataa
    13141 ttgaagacag tgccatctca ccatctccag cttcgccgac ataagtgatt ggataataag
    13201 aaccaaggac agttatttgt tcccgcaagt tattgcgaat gaggtattcg tcttcgatga
    13261 taaaaacttt atacatgttt gtttcctcca attgttttgg gagatagagg gatcatcagt
    13321 gtgacagttg ttccttctcg gtatttgctg tcaatcgtga taggtttttg atcatttccg
    13381 taataaagaa ggagacgctt gtttacgttg taaagtccgt agccgatact gaaatctgaa
    13441 tcgatccctt tggctaactc tttacgtatt tctgccagtt tttctgcagg gatgccaatc
    13501 ccgttgtcta taactttgaa ttggatcatc tcatcaattt tgcatgtggt aatacgaata
    13561 aatcctttgt gacggacgta cttcgttcca tggtatacag cattttcaat caatggctgc
    13621 aagatcattt ttaaaataga aacaggaaaa agtgtttctg gtatatcgat cttatagtca
    13681 agcatatggc cgtatcttat ttttaagatg actagataat cattgatatg gctaatttct
    13741 ttttcaagag gaatccagtc ttgtcctttg cttaaagacg tacgaaaaaa gccggaaagt
    13801 gcatgggtca tagtcagagc ttcttcaaag cagtcttgtt cgatcaaaga aataatggca
    13861 tccaaactgt tataaataaa atggggagta atctgagctt gcaatacacg aacttcactt
    13921 tgtgctaaat ggtattgttt caaagcgttc tcccgtaaag actggtttag atcttccgcc
    13981 attttattca tgctagcggt aagtgtttgt aattccagtg tttccggcag ggttaaacga
    14041 aagccaaggt tccctgaaga aagttgttct gacatctcga tcagctgatt gatcggttct
    14101 tggacctttc tctctaaaaa gcgacgattc tttttcgcat agaaaaaaat ggtactaaga
    14161 actccaagtt ccactattgt taatagaatg agcgagcgga caagtgtcgt attttttttg
    14221 cttgctaagt cgatctccac acgaacaaaa tcttgtaaga tatctgacag cagttgagtg
    14281 acagaatcta cctgattgat gatctcttca ttggtatcca taaaatcttt tgccaagata
    14341 ttattgatca gctcgttttg ataatcttcc agtgtatcga tcgtacgtag cgatacatct
    14401 aacgtactaa tttctttaag tgtatttgta ttatttttga tctctcgaat atcattccta
    14461 agttcttcta ggatattttc ctcattgaac tcatctacgg ttacttggcc agaaatcaag
    14521 tcccacatac tttccaaaac tttttgatca accttcaaag caattgcatc tgctttttgg
    14581 atatttgcga tcgtctgttg ataaatcaaa agctgttgcg aatataacag tatgctcagt
    14641 agcaaaggga taacagcaaa tagaagcatg gctttgtttg cttgggcaaa aatctgtttt
    14701 atgcttaaat tttttgtcat gatacttctc cagtcaataa tttctggttg ggatgttagc
    14761 tatattctgc tgggaaaaga cagtctcgga aataaaaata tctttaggta atgtttttcc
    14821 tgtttgaata tttccaaaat aacgagtaac ggtattggct acataccagc cgacatcagg
    14881 tttacactcg actacacaat tgacgattcc tgagcgtagc ttctcgatca tatcttcttg
    14941 tccatcaata gttacaatga tcaaatcttc tccaggtttg attgatgttt cttttatcgc
    15001 agctaaagca ccgtgagtca tttcatcact atggctgtat aatacatcga tttcggataa
    15061 atcatttgtt tctaaatatc gtttcatctg gtcttttcct ttcatccgaa taaaatcacc
    15121 gacaattcgg tgaacaatag tcatcggagg tttatggtcg atggttccat ctttttttag
    15181 ggttggttta cgattgattg attccaaaaa tccttcggag cgtagcttgg tcggtgtgga
    15241 gttctctgaa ccagcaagtt ctaggattct gatttcctgt ttctttgaat ttgtgaagtg
    15301 attgctgaca aataaacccg cccgatttcc ttctgctttg aaactggggc caatgtgggt
    15361 gacgaaaagc tcgggatcat tcgtttgaat atgtctgtcg acaagtatga cagggatccc
    15421 tgctcgcttt gcttctttta atacagagtc ccacccggat tcttgcaaag gagtaaaaat
    15481 gatcatatcc actttatagg caatgaacgt ccgtatatct tggatttgcc gttcctgatt
    15541 catgtagcca tttcggtaga gaacttggta atcttctttt tcaagcgcag tgataattga
    15601 ttcagtatgc tttttccgcc agctactctc cgttcctgat tgcgaaaaac caacgactag
    15661 tgggtcaggt gcttcctttg tattaggtgt acaccccatc atttggtaag cgattacaac
    15721 aagggctaaa aaaaataaaa cacgtttcac tttaaatcac tccatctgga tttgttagta
    15781 ctatcttatc agataaaagg gaaaatcaaa gtcaaaagga atcaatcgaa aaaaatccaa
    15841 gaaacttaaa aaaatccaag acaaaaaaag tacacctaaa aaatttaaag taaatcccat
    15901 aaaaaaatca atgttaacgc tttctttatt agctatactc ggtgtatcaa ttgaaatggg
    15961 ggataaaaaa tgaaaaacaa atttagaaag attgttggga ctatcgctat tttgaccgca
    16021 agcattctat taagtgcgtg tggcaattca ggatctgcag atgatagtac ggggtatgta
    16081 ggaatcgcta tgccgaccaa atcagcagaa agatggatcg ctgatgggaa caacatggtc
    16141 tcggaattag aaaaactggg ttataaaaca gatcttcaat atggagaaga taaagtagaa
    16201 aatcaagtag cacaaataga aaatatgatt acaaaagggg tagatacact tgtgattgct
    16261 tctatcgatg gttcggcttt aactgatgtg ttagctaaag caaaagaagc agatatcaag
    16321 gtgatcgcct atgatcgttt actgatgaat tcagaaaatg tcgattatta cgcgactttt
    16381 gataattttg gagtaggtgt atcgcaagca gcttatattg aagagcactt aggattgaaa
    16441 gaaggaaaag gaccattcac gattgaatta tttggtggct cgcctgacga caacaacgca
    16501 ctaatcaatt acaacggagt gatgtctgtt ttacaaccat atatggacaa cggtcagttg
    16561 gtcgtgccat ctggacagac gagtttcagt caaatcgcta cgcttcgctg ggatggttct
    16621 acggcgcaag caaggatgga taatctgtta agcgcaaatt atactgacca aacattggat
    16681 gctgttttgt cgccatatga tccaataagt ttaggaatca tttcgtcatt aaaaggcgtg
    16741 gggtatggga gtgaaagtaa acctttacct gtgattactg gacaagatgc gacagttgct
    16801 ggcgttaaat cgatcattgc cggggaacaa acacaaacga tctttaaaga cacacgtatc
    16861 ttagcaaaaa atacgattga aatgatcaaa gcaatcagtg atggagaaga ggttccggtc
    16921 aatgatactg aaacttatga taatggggtg aaaactgtcc ctacgtattt agcaaataca
    16981 gtatctgtcg ataaagataa ttatcaagca gagctgatcg acaccgatta ttataaagaa
    17041 tccgatctga aaaattaaga ggaaccaaac aaggaactaa agagatctaa gtcagagaga
    17101 ggataagaat tatggcggat tgtattttaa aaatggcaga tatcaccaaa aaattttctg
    17161 gaatccgagc gttagattgc gtgaatctga tgattgaacg tggggaaatc catgcattgt
    17221 gcggagaaaa cggagcggga aaatcaacat tgatgaatgt cttgagcggc ttgtatccat
    17281 atggctccta tgacggggaa atcttttatg atggaaagct gtgtcaattt aaagatttga
    17341 aagatagtga agaaaaaggc atcgtcatca ttcatcaaga attagcacta agtccttatc
    17401 tttcaataaa agagaatatt tttctcggaa atgaacaagc agaaagaggc gtgattgatt
    17461 gggaactgac agaaaagaaa acatatgaac tgctgaataa agtcggtttg tctatcgacc
    17521 cgaatcttct agtatcccag attggcgtag ggcaacagca actagttgaa atagctaaag
    17581 cattatcaaa atccgtccgt ctgttgattt tggatgagcc taccgctgca ttgaatgaag
    17641 aagaaagcgc gaatttgctg agattgatcc gagagttgaa agagcaaggt gtaacctcca
    17701 tcattatttc acataagctg aatgaaatcg tggatatcgc agatcgaatc accattttac
    17761 gggatggaca gacgattgag acactagaaa atgaaatgat cagcgaagag catattattc
    17821 gcggaatggt gggcagagat ttagttgatc gttatcctga aagaaaaccg gatattggag
    17881 aagtttattt taaagtagaa gactggacgg tctatcaccc tcttgatcac gagcgaatca
    17941 taaacgacca catcaacttg catttgaaaa aaggagagat tgtaggaata gctggactga
    18001 tgggagcggg cagaactgaa ttcgctatga gtctatttgg acattcttat ggaagcaaga
    18061 tatccggacg tatattaaag gaaggggcag agatatcagt taagaccgtt ccttcagcca
    18121 ttgcaaacgg actcgcttat gtctcggagg accgaaaatc tttaggactg aatctgctga
    18181 tggatattcg ggaaaacgtg acgctgagca gtttggataa aataagtcaa aaaggagtat
    18241 tagataaaga aaaagaagta actgaggcag aaaatttccg gaaaaagatg cgtataaaaa
    18301 caaactccat ccatcaaatt gtcagtagtc ttagtggtgg gaatcaacaa aaagtcgttt
    18361 tatccaagtg gctgatgaca caacccgata ttcttttttt ggatgagcca acccgtggga
    18421 tagatgttgg ggcaaaatat gagatctaca cgattatcga agaaatggca gcaaatggca
    18481 aagctgtctg tcttatctct tctgaacttc ctgaaatatt agggatgtgt gatcgaatct
    18541 atacgatgaa cgaaggacgt attacaggag aagtctcgcg agaagatgca gatcaggaag
    18601 tcttgatgaa attaatgacg aaagaagagg tgggttaagt tggaacaggc aaaaaatctt
    18661 acggataaaa aagagaaaaa actaaatttg aaaagtagag cagctgatct ttttggtcgc
    18721 tacagcatgg tgatcatttt agcgggacta cttattgcgt ttcaattgat gacggatggt
    18781 attttctggc gtccattgaa tataactaac cttgttctgc aaaatagcca tatattagta
    18841 ctagctgcag ggatgttatt agttgtgctc ttaggtcatg tggatttgtc ggtagggtca
    18901 gtgatggcgt ttgttggtgc aattgcgggc atgatgatgg tcaatcgcca aatgaacccg
    18961 tggatgacag ttatcgcatg tcttgcagta ggcgggctga tcggcgcatg gcaaggtttt
    19021 tgggtagctt atgcagggat accggcgttt atcgtgactt tagcaggatt gctgatgttt
    19081 cgtggactga cacaagttgt tctaggaggc caatctctag caccttttcc agctgttttc
    19141 caaaaaatgt ctacgggtta tttaccagac ttaagcgaag gaagcagcct tcacttcttg
    19201 acattgattt taggaatgat catcacgatc gtgttagttg ttggtcaatg gcgaacaaga
    19261 aatcgtcgga aaaatcattt atttgaagtg gaatctccaa cagctttctt ggtaaaggcg
    19321 gtatttacag cagtcgttgt actaggaata acttatatct ttgcctctta tcaagggtat
    19381 ccgattattt tgatcattct tggtttgatc gttgcagcat atgggttttt gacaaataaa
    19441 acagtagcag gtcgacagat ttatgcgacg ggcggaaatc gaaaagcggc tgaactatca
    19501 gggatcaaaa caaagcgaat cacgttctgg gtattcgtca acatgggagt catggcagct
    19561 ttagctggat tagttttagc tgctcgattg aatgcggcaa cacctcaagc aggtacatcg
    19621 atggaactgg atgcaatggc ggcagtatat tttggtggtg cttcgacttc cggcggcatt
    19681 ggaacaatca tgggtgcgat tgtcggtggc ttggtcatgg gtgtattgaa caatggcatg
    19741 tcgattttag gcgtcggggt agactggcag caggcaatca aaggattgat cttgctttta
    19801 gccgttgtgc tagacttgta taataagaaa aaacgaacaa cgtaggagaa cgccggttat
    19861 tcagaaaaaa gactgtgaca agacatttgt cacagtcttt ttttcgctaa aagcgaacat
    19921 tcattgtttc gtttgtatag actaaaaata atatatgttt ttttaccatt cgtataaatt
    19981 ctctttttgc tcttgatttt tttgcttaat tgagttaaat tacatagggc gctaaaaaaa
    20041 gccttgatta aattaataat gttcgtgttt tgctggatgt gaacatcagg aggataatgt
    20101 aatgaaagta tttgattacg aagatattca attaattcct aataaatgta tcgtaaatag
    20161 ccgttctgaa tgtgatacaa cggtcacatt aggtaaacat acattcaaaa tgccagtagt
    20221 gcctgccaac atgcagacga ttattgatga aacaatcgca gaattcttag cggaaaatgg
    20281 ttatttctat atcatgcatc ggtttgatga agcagctcga attccgttta tcaaaaaaat
    20341 gaaaaagcgt ggattgatta cctcgatcag tgttggcgtc aagaaagagg aatattcctt
    20401 tatagagaag ttggcagaag aatcgctaaa tcctgattat atcactatcg acatcgcgca
    20461 tggacatgct aactcagtta ttgatatgat tcagcatatt aaaaaatatt tgcctgaaac
    20521 ttttgtgatt gccggtaatg ttggtacacc ggaagccgtc cgtgaattgg aaaatgcggg
    20581 cgcagatgca acaaaagtcg ggattggtcc aggaaaagta tgtattacaa aaatcaagac
    20641 aggctttggg actggaggct ggcaactggc agctcttaga tggtgtgcga aagcagctcg
    20701 caagccgatc atcgctgacg gtgggatcag aacacatggc gatattgcaa aatctgtccg
    20761 ttttggtgcg acaatggtca tgattggttc attgtttgcc ggtcacgagg aatctcctgg
    20821 cgaaacaaaa gtcgaaaatg gaatcgtgta taaagaatac tttggttctg cttcagaatt
    20881 ccaaaaaggc gaaaaacgaa atgttgaagg aaagaaaatc tggatccaac acaaaggatc
    20941 tttgaaagat acactagtgg aaatgcaaca agaccttcaa tcatctattt cttatgctgg
    21001 aggaagagat ctggaagcca ttcgaaaagt tgactatgtc atcgtgaaaa attcgatttt
    21061 taatggagac acaatctaaa aaataaataa aatattagga tgttgtgaaa aagttgtcct
    21121 gcattaagta ataagaacag ccaaacaaaa atagttcttt atatgttttg tttggctgga
    21181 attattatcg cagatacaag cttttgaaca tcattattca gaataaggga ctgtgataag
    21241 atttttgtca cagtccctta tgttttgcaa aaaaagtttt tcttttttct cgtaaattcg
    21301 gtaaaatcaa aagaaaaaat agacaataga aaagagaagc ttatgtggga aaaagtagag
    21361 aaaaaacaag gaccgattta tcgtcaaatt atggaacaga ttatgagaga aatcgaaaac
    21421 ggaaggatca atcctggaag tcaacttgaa tcagaacgaa aactagggca actttttggt
    21481 gtgaatcgtt ccaccgttgt ccatgcattg gaagagctta gggagcttgg ggtactaacg
    21541 agcaaacgtg gcagcggatg gtttgttaat cagactgaat gggggaaatt cgctgttcca
    21601 agaatcgatt ggcggcaaat gatctctcct cgttatgagc agacggactg gtatgagcaa
    21661 aaaataagag aagcaaagca gatccaaaaa acatcgtttc ttgatttata cgctagcgag
    21721 atgccagatg agcttttgcc gaattttgag tttccttctt attctcttga agaaatctta
    21781 gcggaagaaa aagaaatgga tcttttaggc tatactccgt tacgtaacaa aatcaaacgg
    21841 tatttggaaa gtaaatttga attggcattt tcccccaacc agcttcttgt gactggtggc
    21901 ggtcagcaag caatcttttt gattttacaa acgattcttt cagctggaga ggggatagct
    21961 gtagagtcac cttctttttt ttaccgatta cctcttttca aagcttcagg aacgcgttta
    22021 tttgggatac cgatggatga agaagggatc gacttagaaa aactgaaaga atcgatccat
    22081 aaaaataaat tgaaagcagt gcttatcaac ccgaattttc aaaaccctac aggaaaagtg
    22141 atgagtcaaa aacgacgaga aaagctagta gaattgtgtc gacagtatcg gcttccaatt
    22201 attgaagacg atgtatttac ggatctttca tttctaaacc aagaagaagt ccttcctata
    22261 cgaacgattg atccggataa tgtgttatac gtcggttcgt tatctagggt tttaggaaaa
    22321 acaacgaaaa tcggttgggt gataggacct gagactttta tctcgcaact agctcaagcg
    22381 caagaaatga tggagttttc gatgaatatc cttacacaaa tcacagtggc taatgtattt
    22441 aatgaagcaa ttgatttgaa aatgcaagat gtaagaaaaa agttgaaaga aaaaagccga
    22501 ctgataagcg cttggggaga ttcacaatcg tttttcaagc ttcattcaat tttaggtggt
    22561 tactatgcct ggattacttg ggatgggaaa aaactagacc ccgaactagc agaaaaagta
    22621 gtagatatgg gtcttggaat tgcaccaagt ctactttttg ggcaagatat aaatggaatc
    22681 agaatcaatt acagtaggct ggatgaaaac caaataccgt tgttttattc aaagatgaaa
    22741 attctagaaa agtggttaac agaataattt cacaaagtaa atagattaaa gaatataaca
    22801 gcttggattt aaatttcaag ctgtttcttt tttttcggaa aaagaatacg atataaagaa
    22861 actaagaaaa aaccattatt tgtacgtccg ttcacgatgt gtaaataaaa taaatgtttg
    22921 caacaaaact gtataacacc aacgttttta aatagaaaca gatggttgta agtttgatta
    22981 gaaaatagaa aaaatgcaga tgaaatataa aacgctttcc ttattgtgat tttattcact
    23041 aatgctgtta atatgcggat tggcataatt tcctttcatc gtgaaaattt gtgcttttag
    23101 cttgtatttc tcataaatcc atgataattt attaatgaag acggttaaat agtgatacag
    23161 ttttattcac tgtttcaatc gcagaatgaa gagattttct tatagatgag agaggaatgt
    23221 gtgacaaatg gcaaacaaaa aagcaccgat cgacttccaa gcactgctag atgaagtcaa
    23281 tgcagatttc ccagtatatc aagcacttga tcaagatgga aacgtagtta acaaagattt
    23341 agtacctgat ttatccgatg atgaactcgt tgaattaatg actcatatgg tatggtctcg
    23401 tgtattagac caacgttcaa cagcgctgaa ccgccaagga cgcctaggct tttttgcacc
    23461 aactgctgga caagaggcaa gtcagctggc tagtgcttac gcatttgata aagaagatgt
    23521 tcttcttcca ggttatcggg atgtacctca actagtaaaa catggtttac ctttatctca
    23581 agctttttta tggtctcgag gacatgctgc aggtaattct tatccggaag aattgaaagc
    23641 tctaccacca caaattatca tcggtgcaca atatgtacaa gcagccggtg ttgcattagg
    23701 tttgaagaaa cgaaacaaga aaaatgtcgt cttcacttat actggtgatg gcggttcatc
    23761 acaaggtgat ttttacgaag caatcaattt cgctggcgct taccatgcaa atgccgtatt
    23821 ttatatccaa aacaacgggt atgcgatctc tacacctcgt gaaaaacaaa ctgcagcaaa
    23881 aacacttgca caaaaagctg tcgcagctgg gattcctggt attcaagtcg atggaatgga
    23941 tccgcttgca gtatatactg tatctaaaat ggcacgggaa tgggcgatca gtggaaatgg
    24001 accagtccta atcgaaacac tgacttatcg ttatggacca catacactat ctggtgacga
    24061 tccaacccgc taccgtacaa aagacacaga tgacgattgg cagaaaaaag atccattgat
    24121 ccgcttccgt aaatatttga cggaaaaagg actttggtct gaagaaaaag aagaacaagt
    24181 catcgaagca actaaagaag aaatcaaagc agctattgct gaagccgaca aagtaccaaa
    24241 acaaaaggtt tcagatttct tgaaaaacat gttcgaagta tctccacaat caatcaaaga
    24301 acaaattgca atttatgaag cgaaggagtc gaaataatca tggcacaaaa aacaatgatc
    24361 caagcaatca cagatgcctt agcacttgaa ctagaaaatg acgagaatgt cgttgttttt
    24421 ggtgaagatg ttgggaaaaa cggaggagtt ttccgggcaa ctgaaggatt gcaggaaaag
    24481 ttcggagaag accgagtatt cgatactcct ttagctgaat ctggaatcgc aggattgagt
    24541 ttcggtcttg cgttagaagg atttcgtcca gttcctgaaa tccaattctt tggttttatt
    24601 ttcgaagcaa tggacgaagt agtagctcaa atggctcgta caagatatcg aatgagcggc
    24661 actagaaatc tacccatcac aattcgttcg ccatttggtg gcggtgttca tacaccagaa
    24721 ttgcattcgg ataatttaga aggtttgatc gctcagtctc ctggtattcg agtagtgatt
    24781 ccttccaacc cttatgatgc aaaaggacta ttgatttctt ctattagaag taacgatccg
    24841 gttgttttcc tagaacacat gaaactatat cgctcattcc gagaagaagt gccggatgaa
    24901 gcttacgaag ttcctttgga taaagcagca gttactcgtg agggaacaga tgtatctatt
    24961 atcacatacg gagcaatggt gcgtgaagca atcaaagcag cagacaatct tgcaaaagaa
    25021 aatatatctg ttgaaatcat tgatcttcgt acagttgcgc cattagatgt agaaacgatc
    25081 attcaatccg tggaaaaaac tggtcgcgta gttgttgtcc aagaagcaca acgacaagcg
    25141 ggcgttgccg cgcaagtagt ttctgagatc tcagaacgcg ctatcctttc attagaagcc
    25201 ccaatcggac gagtttctgc acctgataca gtcttcccat ttggacaagc agaaaatgtc
    25261 tggttgccaa atgcaaaaga tatcgaagac aaagtaaaag aaatcgcgga attttaagaa
    25321 aagaagggac gaaacaaagt catggcttat caatttaaat tgccggatat cggtgaagga
    25381 atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat
    25441 gatacattac tagaagttca aaatgacaaa tcagtagaag aaatcccgtc acctgttaca
    25501 ggaacggtta aaaatgtgat tgttccagaa ggtaccgtag caaatgttgg ggatgtatta
    25561 gtagaaatcg atgcaccagg acacgaagac aatgaagggg acagtggtgt agccgcagaa
    25621 tcccaaactc cagctaaacc ggctgctgaa cccactgttg atacagaatc agctggatcg
    25681 tcaagcgaag gagtcttcca atttaagtta ccggatatcg gtgaaggaat cgcagaaggt
    25741 gaaatcgtca aatggttcgt caaaccagga gatacgatca atgaagatga tacattacta
    25801 gaagttcaaa atgacaaatc agtagaagaa attccgtcac ctgtcacagg gacagttaaa
    25861 aacgtgattg ttccagaagg cactgtagca aatgttgggg atgtattagt agaaatcgat
    25921 gctcctggac acaatagtgc accgtcaact agtgcgccat cagctgaagc accaaaagaa
    25981 aaagtggaaa catcaggttc agcaagtgtc gttgaagctg ccgatccaaa taaacgtgta
    26041 ttggctatgc catctgttcg tcaatttgcc cgtgaaaaag atgtagatat ctcacaagta
    26101 actgcaactg gaaaaggtgg cagagtaaca aaagaggaca tcgaaaactt cttggcaggt
    26161 ggtccaagca gtgcaccagc gaaatcagaa gcgccagaag cggcagcacc gaaagaagca
    26221 gctcctgcag cagaaagcaa accagctgca ccagcaaaac cattcaagtc caacttagga
    26281 gacttggaag aacgtgtagc aatgacgcca acacgtaaag ctatcgctaa agcaatggtc
    26341 aacagtaagc atacagctcc acacgtgaca ttgcatgatg aagtagaagt gtctaaacta
    26401 tgggataatc ggaaacgatt taaagaagtt gctgctgcaa atggtacgaa acttactttc
    26461 ttgccatatg tcgtgaaagc tttaacagcg actgttaaga aatacccagt attgaatgca
    26521 tctatcgacg atgcaaacca agaaatcgtt tataaacatt actacaatat cggtattgca
    26581 actgatacgg atcacggact atatgtacca aatgtgaaag atgcagaccg caaaggaatg
    26641 tttgctattg cagacgaaat caatgagaaa gcaaaattag ctcatgacgg caaactatct
    26701 gcagaagata tgcgcaatgg aacaattacg atcagcaata tcggatctgt aggtggcggt
    26761 tggtttacac cagtcatcaa ctatccagaa gtagctattt taggtgtagg aacgattgca
    26821 caacaaccaa tcgtaaatgc tgaaggcgaa atcgttgtcg gtcgtgtgat gaaactgtca
    26881 ttaagtttcg atcaccgtat cgtagatgga gcaactgcgc aacaagcaat gaataatatc
    26941 aaacgtcttt tagctgatcc agagctatta atgatggaag gatgataaac aaatggtagt
    27001 tggagatttt gctgtagaat tagatacagt tgtaattggt gcgggacctg gtggctatgt
    27061 agctgccatc cgcgctgctg aaatgggaca aaaagtagca atcattgaaa gagagtatat
    27121 tggcggtgtt tgtttgaatg tcggctgtat tccttcaaaa gcactgatag ctgcaggaca
    27181 ccattatcaa gagtcactag attctgaaat gtttggagta actagtgaaa acgtcaaact
    27241 agattttgca aaaacacaag aatggaaaga aaacaaagtt gtccatacgt tgacttctgg
    27301 tgttggtttc ctattaaaaa aacataaggt tgaaacaatc gaaggagaag cgttcttcgt
    27361 agatgatcac acactacgcg tcattcatcc agattcagct caaacttatt cgttcaataa
    27421 cgcaatcatc gcaacaggtt cccgtccaat cgagattcca ggatttaaat tcggaggacg
    27481 tgtgttagat tctactggcg gattagcatt gaaagaagta cctaagaaat ttgtgatcat
    27541 cggtggcggt gtgattggtg ccgaattggg cggagcgtac gcaaacttag gagcagaagt
    27601 aacgattctt gaaggctcac cacaaatttt accaacttac gaaaaagatc tagtaaaatt
    27661 agtagaagat gatttcaaga aaaaaggcgt aacagtcgta acgaatgcaa tggcgaaaga
    27721 agcagtcgat aacggtgaca gcgtgacagt gaaatatgca gtggacggga aagaagaatc
    27781 cgttactgct gattatgtga tggtcactgt aggtcgtcgt ccgaatacag atgatatggg
    27841 cttagaacaa gcgggtgtcg aagtaggaga acgaggattg atcacggtgg ataaacaagg
    27901 acgtacgaac gtacctaaca tctatgccat cggtgatatc gttccagggg cggcattagc
    27961 acacaaagcc agttatgaag caaaaatcgc tgctgaagca atctctggca aaaaagtagc
    28021 tgtagattat aaagcaatgc cggcagtagc cttcactgat cctgaattgg cttctgtagg
    28081 tatgacgatc aaagaagcaa aagatgctgg tatcgaagca aaagcatata aattcccatt
    28141 ttcagggaac ggtcgggcat tatcgcttgg taaaacagaa ggattcatcc gtctagttac
    28201 gacgatcgaa gataacgtgc ttattggcgc gcaaatcggt ggagtcggcg caagtgatat
    28261 ggtctctgaa ttagctttag caatcgaatc aggaatgaat gctgaagata tcgccttgac
    28321 gattcatcct catccatctc ttggcgaaat cgtcatggat gcttcggaat tagcattagg
    28381 tttaccaatc catatttgat ttgaataata caggcgaaac gcaactgaaa aatcagttgc
    28441 gttttgtttt ttagaattat caataatatt attatgtaaa cttaaaaata tgacaacttc
    28501 ttggtttatt accacttcct atgctacact gaagaggaat atgaaatagg agagttgaag
    28561 atgcaagaat atcagtatcc acttgattta gattggacaa cagacgaaat ggtcattgta
    28621 atgaagatgt gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt
    28681 ttgaagactt atcagcagtt taaaactgtt gtcaaatcca ttggtgagga aaaacgattg
    28741 ggacgggaat ttgaaaaagc ttccggttat tctttatatc gtacagtgca gcaagcaaaa
    28801 aaaaatccaa acaaaacttt gagaatgaac gggtgagata aatggaacag atgattgatc
    28861 atatcaagtc ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa caaacattaa
    28921 caatctcgca aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga aacatccaga
    28981 attttctaat cgaaaaaatc cattcctttg atccagaggc gaaaatattg gcagaagaag
    29041 aagggtgtaa tcaattatcg gatctcgatg gaagagtctt tatcatcgat cctattgatg
    29101 gcaccttgaa ttttgtgatt gaaggggaaa atttttgtat tatgctcgct gtttatgaat
    29161 ccggcgttgg aaagcttggt ttcatttatg acgtgatgag aaatgaactt ttttggggtg
    29221 gaaaaggaat aggcgtgtat aaaaatgatg agccattgcc tagaccagca aataaatcct
    29281 tatcggaagg cttacttggt gtgaatagtt atcttttcgg tcataataaa tatggaatcc
    29341 gagaaattgc ggaaagcagc atggggacac gtatgtacgg atgcgctggt cttgaactag
    29401 tggcaatctt gaaaggaaac cacattgctt atatttcaaa ccttagccct tgggattatg
    29461 cagcgggaaa tgtcttattg gaagagtttg gtatggtaac aagcggtctt tttggtactc
    29521 ctttgacatt tgaaaaaagg gaatactatt tagcagggac ttcttgtgca tatgcagaaa
    29581 tcgcagacct tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca caaaaatgat
    29641 aaaaacgagc aaaagattaa ttttctcgaa aaaatgaaaa cattttacta tatttcatga
    29701 aaaaagtctg ctataattag cagtcgagta aaatttgccc tgtcataaag aaccaataaa
    29761 aaaggagtta gttacattga attatagaaa cgatatccgt aacgtcgcaa ttattgccca
    29821 cgttgaccac ggaaaaacaa cattagttga tgaactatta aaacaatcag atacattaga
    29881 cgcacatacg caattgcaag aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg
    29941 tatcacgatt ttagccaaaa acacagctgt agactataaa ggtattcgcg taaacatcat
    30001 ggatactcca ggacacgcgg acttcggtgg cgaagtagaa cggatcatga aaatggttga
    30061 cggcgttgtg ttggtcgtag atgcgtatga aggaacaatg cctcaaacac gttttgtatt
    30121 gaaaaaagca ctggaacaac atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc
    30181 ttctgctcgt cctgagcatg tcgtagatga agttcttgaa ctattcatcg aattaggagc
    30241 agatgatgat cagttagact ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag
    30301 cttgtcagat gatccagcag atcaagaacc aaccatggca cctatttttg atacaattat
    30361 tgaaaaaatc cctgctcctg tcgataacag cgacgaacca ttacaattcc aagtttcatt
    30421 acttgactac aacgattatg taggtcgtat cggaatcgga cgtgtattta gaggaacaat
    30481 caaagtagga gaccaagtag cattaattaa attagacgga acagttaaga aattccgtgt
    30541 aacgaaatta tttggtttct ttggactaaa acgtctagag attcaagaag caaaagctgg
    30601 agacttgatt gctgtctcag gtatggaaga tatctttgtc ggtgaaacag ttactcctgt
    30661 ggatcatcaa gatgcgttac ctatccttca cattgacgaa ccaacattgc aaatgacttt
    30721 cttagttaat aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat
    30781 tgaagaacgt ttgatggcag aacttcaaac agatgtatcc ttacgagttg aaccaacaaa
    30841 ctctccagat gcatggacag tttctggtcg tggagaattg catttgtcta tcttgattga
    30901 aaacatgcgc cgtgagggat acgaattgca agtatctcgt ccagaagtta ttgaaaaaga
    30961 aatcgacggt gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac cagaagaata
    31021 tatgggtagt gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag atatggtcca
    31081 tacaggaaac ggacaaatcc gtttgacatt cttaacacct gcccgcggtt tgattggtta
    31141 ttcaactgaa ttcctttcaa tgacacgtgg ttacggaatc atgaaccata cttttgacca
    31201 atatctgcca atgcttcctg gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat
    31261 cgatacagga aaagcaacga cttattctat catgagtatc gaagaacgcg gtacagtctt
    31321 cgtagaacca ggtacagaag tatatgaagg aatgattatc ggggaaaaca gccgtgataa
    31381 tgatttgact gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa
    31441 agaccaaaca tctgtcatca aaaaaccaaa acaattgacg ttggaagaat ctttggaatt
    31501 cttgaatgat gatgaatatt gcgaagtaac accagaaagc attcgcttga gaaaacaaat
    31561 tttagagaaa aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg taaaaggatg
    31621 aagagagaaa ccagcttgtt ata
//
LOCUS       DACDNO010000030        31428 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDNO010000030 DACDNO010000000
VERSION     DACDNO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.030"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..109)
                     /locus_tag="IWI22_001450"
     CDS             complement(<1..109)
                     /locus_tag="IWI22_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9168848.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVK"
     gene            complement(139..1230)
                     /locus_tag="IWI22_001451"
     CDS             complement(139..1230)
                     /locus_tag="IWI22_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9168849.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(1331..1765)
                     /locus_tag="IWI22_001452"
     CDS             complement(1331..1765)
                     /locus_tag="IWI22_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9168850.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(2349..3806)
                     /locus_tag="IWI22_001453"
     CDS             complement(2349..3806)
                     /locus_tag="IWI22_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9168851.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(3803..5698)
                     /locus_tag="IWI22_001454"
     CDS             complement(3803..5698)
                     /locus_tag="IWI22_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9168852.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(5756..7531)
                     /locus_tag="IWI22_001455"
     CDS             complement(5756..7531)
                     /locus_tag="IWI22_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9168853.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(7581..9077)
                     /locus_tag="IWI22_001456"
     CDS             complement(7581..9077)
                     /locus_tag="IWI22_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9168854.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(9271..10893)
                     /locus_tag="IWI22_001457"
     CDS             complement(9271..10893)
                     /locus_tag="IWI22_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9168855.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(11343..12656)
                     /locus_tag="IWI22_001458"
     CDS             complement(11343..12656)
                     /locus_tag="IWI22_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9168856.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(12901..13125)
                     /locus_tag="IWI22_001459"
     CDS             complement(12901..13125)
                     /locus_tag="IWI22_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168857.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(13145..13513)
                     /locus_tag="IWI22_001460"
     CDS             complement(13145..13513)
                     /locus_tag="IWI22_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9168858.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(13709..14419)
                     /locus_tag="IWI22_001461"
     CDS             complement(13709..14419)
                     /locus_tag="IWI22_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168859.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(14437..14933)
                     /locus_tag="IWI22_001462"
                     /pseudo
     CDS             complement(14437..14933)
                     /locus_tag="IWI22_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(14970..15422)
                     /locus_tag="IWI22_001463"
     CDS             complement(14970..15422)
                     /locus_tag="IWI22_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9168860.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(15557..16162)
                     /locus_tag="IWI22_001464"
     CDS             complement(15557..16162)
                     /locus_tag="IWI22_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9168861.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(16612..17232)
                     /locus_tag="IWI22_001465"
     CDS             complement(16612..17232)
                     /locus_tag="IWI22_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9168862.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(17234..18103)
                     /locus_tag="IWI22_001466"
     CDS             complement(17234..18103)
                     /locus_tag="IWI22_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9168863.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(18126..18533)
                     /locus_tag="IWI22_001467"
     CDS             complement(18126..18533)
                     /locus_tag="IWI22_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9168864.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(18552..19841)
                     /locus_tag="IWI22_001468"
     CDS             complement(18552..19841)
                     /locus_tag="IWI22_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9168865.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(19868..20170)
                     /locus_tag="IWI22_001469"
     CDS             complement(19868..20170)
                     /locus_tag="IWI22_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9168866.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(20195..22288)
                     /locus_tag="IWI22_001470"
     CDS             complement(20195..22288)
                     /locus_tag="IWI22_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9168867.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(22777..23505)
                     /locus_tag="IWI22_001471"
     CDS             complement(22777..23505)
                     /locus_tag="IWI22_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9168868.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(23651..26272)
                     /locus_tag="IWI22_001472"
                     /pseudo
     CDS             complement(23651..26272)
                     /locus_tag="IWI22_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(26386..27717)
                     /locus_tag="IWI22_001473"
     CDS             complement(26386..27717)
                     /locus_tag="IWI22_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9168869.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(27683..29161)
                     /locus_tag="IWI22_001474"
     CDS             complement(27683..29161)
                     /locus_tag="IWI22_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9168870.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(29466..31301)
                     /gene="tdc"
                     /locus_tag="IWI22_001475"
     CDS             complement(29466..31301)
                     /gene="tdc"
                     /locus_tag="IWI22_001475"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9168871.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
BASE COUNT         9124 a         6694 c         5211 g        10399 t
ORIGIN      
        1 tttttactaa tacatcataa aaatgagtca gttcacttaa ccacaaacct gtaggacgct
       61 cgatcttttc gtacttttct acatttgtta atacgatcaa tactttcatt ttcccatcct
      121 ctttctataa aaattgcgtt attgataacg tgctttcaag aaattatata atgggcttgg
      181 caaataaagc tctttatatg cttcttcaaa aggtttcgtt acaaattcat cctcacggtt
      241 ttccaataat ttctctgccc agtcaggatc gacgatcatt tgctgtccaa ttgctgcaag
      301 ttctgcatat ttcaatacct gctctacatc ttcgcgattg cggatcccac cgacaccaac
      361 taaagggatc tttccttgaa tcgtgttatg gaccacttca agaatcgatt tatcgttgta
      421 tttatcagaa cgagctattc gatcatacat atttaatgaa acatgcagat aatctagttt
      481 tgactcacgt aatcgttcaa gaagccagat cgtgtcttca aaacggatac ctggtgtttc
      541 atattcttct ggtgagaaac gataccctac gatgaatggt tttttagcat attgttcaac
      601 cgctgagaaa accgttgtta ataattcttc aataaaacga taacgatttt ccaagctacc
      661 gccccattga tcctctcgtc tattcgaatg cggagagaaa aattgttgga tcaaatacgt
      721 atttgctcca tgcaactcga ttccatcaaa tcctgattca atcgctctac gtgtggcttt
      781 gccaaatgct tggattgttt caacgatttc ttcttcactc attgctcgtg gtgtttccgc
      841 atcaggacgt tttgctgcaa ctgcacttgc agatacaggc tgttctccag ataaagttgc
      901 tcgacttgtc attcgtccac cgtggaaaat ctgaataatc gcttttgcgc cttctccttg
      961 gattgctttc gctagctcac ttaatcgagg aatcatttca tcatgagcga tgctgagttc
     1021 acctggccag ccttttccga tatcttggac atttgcagca ccagtaataa cagcaccaag
     1081 acctttagaa cgttgtttat aataagtgat ctcatcttcg gtgatcactc cattgaaaaa
     1141 gctgagttgt gtcgtcatag gcgacagcat caatcggttc ttcaattgaa ctcctgaagg
     1201 taaagtaatt gattgatcaa attgtgacat tgtcattcct cccataattc gctctgatta
     1261 acctatcgag aatcatctat cattaaacca ctcgattagt tgtacatgca actaatttgc
     1321 ttaaaaaatt ttattttttt tgtaacttat ctgttgcatc attcattgtc tgggctaatt
     1381 ctttacttgc ttcatttccc aaaactgctt tgtatgactg atgaagttct tcccagctac
     1441 catcgacttc cttagcaaat tggcgtcctt gttctgtaag ataaactaat gacaagcgac
     1501 cttcgctaac tgttttgacg tatcctttat attctaattt ttcaacaaaa cgagcaatcg
     1561 ttgatggggc ggaatagagc atttgtgcaa tatctttttg tggtattcca tctttttctt
     1621 ttaatatcaa taaaataaaa ccataagttg gtgccagacc tgtccttcta aacgcttcat
     1681 cagctaattt attcaacatg cgatctagct ttttcacgct aaagaaaaga cattcatcta
     1741 acgctttttc aattggcttt tccatttcat cccctccatt ggcgtacata tcatagtatg
     1801 atttttattt tttgtcaaaa aacacggaat aatgtccgcc tcctctgtct agatttgaaa
     1861 aaaagaagta gccatcttca aagaatttcg gcttgttttc gacaaaactc cttctggaac
     1921 accgtgaatc agaggttgtg aaaaagtcgt ttagttttga gtatgaagaa aaattttgtg
     1981 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgaaaaact ggcttttgaa
     2041 caccgtttat atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa
     2101 accaaaatag ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg
     2161 tacaccgttt attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt
     2221 tcaaaaaaca gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg
     2281 agaatactgt ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt
     2341 tccttttatt ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat
     2401 tttttcttta accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat
     2461 ccataacgtt ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac
     2521 gtataaccaa ataaatctac tccatcttcg atagcttcct tcatctgctc aatatgctgc
     2581 ttcaaatagt caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata
     2641 gctccgagtc cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc
     2701 aaagcaattc tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac
     2761 ggatttttaa ttcctccagt tattaagttt ccttggaccc cttccaaatt ttctggatga
     2821 gcactgactg cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct
     2881 aagatttctt catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt
     2941 tgcgtatacg ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt
     3001 tgctgctcaa ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga
     3061 tagctgatca tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct
     3121 aaagcacttg ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga
     3181 tggttttctt caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca
     3241 ttgaaagtca tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa
     3301 cgaacgtaaa actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat
     3361 aacggcattt cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg
     3421 tcaaacactt gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga
     3481 ggaaaaattc ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat
     3541 aaacggatgt cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa
     3601 tacttgctgt tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga
     3661 tctgtccatt tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat
     3721 cctttttcat ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat
     3781 aaaaatcctt ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt
     3841 gattattttt cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa
     3901 ccattgtggt cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata
     3961 aatcgccttt acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta
     4021 aattcactgt atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc
     4081 caattgcatg tttcgttgga aaaactgttg aaacttcccc gtttacagga ctgtagattt
     4141 ttccgtccgt tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat
     4201 cgtttacttc agataaatca attatttcac ctgctgcagg agtacttaac actatatcct
     4261 ttgaggattc ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac
     4321 tgatcatcgc taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg
     4381 ggaaagacaa aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta
     4441 ctccaccaaa tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag
     4501 tcacaccata aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag
     4561 ataaactttt tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt
     4621 gcgcagctac ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat
     4681 tgttggtcat tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa
     4741 atccgccgat aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag
     4801 ctgcaatcag ctgacttgca taatctgcaa ttggacctac aactaataaa gtaattggga
     4861 ccatcacgat gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt
     4921 tgcgaatctt aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg
     4981 tacttgtata tcttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac
     5041 tatatgcact gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac
     5101 gatctgtatt gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa
     5161 atgcatcagc tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca
     5221 aactcgtaca taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag
     5281 tcggggcaaa aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag
     5341 ttcctctctt tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat
     5401 acacttcatt tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag
     5461 tgatcacacc ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg
     5521 taaagcgtaa ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa
     5581 atcggacaat atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta
     5641 atgccgtagc aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc
     5701 aattagtacc ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc
     5761 cgagcaaatc actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg
     5821 agacaaaaaa aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat
     5881 actctgtgat ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat
     5941 ttaacggaag cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct
     6001 ttttatggat ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct
     6061 ttttttcttc aactatggtc agcttttcac gcaaaatctg ataaatctcc gaagattcaa
     6121 actcatctag aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct
     6181 cagtagtcaa aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca
     6241 ctggttctat ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta
     6301 gtgtattcaa aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa
     6361 aaggattcaa cgcttcggca agatacaaaa ccaaataagc gctttcagct aaattgggac
     6421 gtaattgaaa atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg
     6481 agatgatctt ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca
     6541 tttgtttgat atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat
     6601 ctaaaaaacg aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt
     6661 tttcatcatt aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt
     6721 cttgattgga aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca
     6781 tcggaataaa taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg
     6841 tcaaaacgag ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg
     6901 atataagaac ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac
     6961 tgatttgttg aggcaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga
     7021 aaattctttt tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca
     7081 gttctaattt aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg
     7141 ctgtttttga aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa
     7201 acaataagta cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt
     7261 tcgtcacctc ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac
     7321 ctgaacgttt atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga
     7381 tttcagtttg gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta
     7441 tccaatgatt cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca
     7501 tttgcttaca cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt
     7561 cacttgcaga ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta
     7621 gactggataa tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg
     7681 ctcccatcat tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt
     7741 cctgccgata cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct
     7801 tttattgcct cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg
     7861 tggattcgat tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat
     7921 aatggtaatt cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca
     7981 attggccaac tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt
     8041 gctccgccag tatctacgcg catttctgct tcttttttat agacagcgct acgataataa
     8101 ctaaatgcaa tataatctac cgtgttagct tgcaagtact gttcgtctcc atctaacatt
     8161 actggtgcac agtcttcttc cttccatatc ctgtaaatat aagctggata atgccctcgg
     8221 cacatgacat cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc
     8281 tctggatcac agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc
     8341 ttagcatttg gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata
     8401 tgatgacagg cttgataaat ctcttttttt gttagtgtag caatgggttc aagtttatct
     8461 ctagcaggga atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc
     8521 tcattaaagg tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca
     8581 taaaattcaa aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat
     8641 gctaaggggg tttcataatg actaagagta attactggct cgatatcata tcgatttaat
     8701 gtattgatta gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct
     8761 cttggaaata ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc
     8821 agtaaacgaa tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa
     8881 tggagtgatt cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta
     8941 ggatgctggt tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc
     9001 aaccatgcac cttcacattg attagcagct actgctccgc cccataaaaa attttctgga
     9061 aatatttcct gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata
     9121 gctcgattca tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga
     9181 atgctactct tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga
     9241 aaacactcgt taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata
     9301 aggtcgtaaa tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac
     9361 ccttaaagct tcacgtggaa cagtgaacgt ctgcttttca ctggacatat tacaaataac
     9421 gagccattgc tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc
     9481 cactaattca tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt
     9541 cttgtacata taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt
     9601 ataatttgga ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc
     9661 gttccattgc ataggcgtcc tagcattgtc tcttcctttc gcattgattg attccaagat
     9721 ttcctctttt gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc
     9781 ttttgcttcc gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata
     9841 gatataaggg gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt
     9901 tcgatacaca tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca
     9961 aaataaacta ttccatcctt catcacctaa agatgtttgc catttggata atactttttt
    10021 cagttcagag atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa
    10081 tcctatgtgt tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag
    10141 tttagcgatt tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc
    10201 aaaagttgct tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat
    10261 ttctttatcc ggttctttgc caatcatatc gatcacgtcc atacgaaagc caccaatacc
    10321 tttatcgatc cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca
    10381 attcaaatca ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc
    10441 caactgccaa gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac
    10501 cggatcacgc caaatataat agtcccgata aggattttct ttatttttac gggcctcaat
    10561 gaaccacgga tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt
    10621 tcttttcttt gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat
    10681 gattgcttga taattactga tatcataccc attgtcatca ttcggagact cgtatactgg
    10741 agaaagccaa attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc
    10801 ccgcaaatct ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata
    10861 aacaactgaa tggtgccacc atttttgttc catgattttc ctcctataaa tggatgtttc
    10921 tattttttgc ttaatcattt attttagtct ctctcactaa aaattgtaac gctttcaaat
    10981 agctattgca ccttgttatc ggtaacagaa taaaacaaaa aaacagctga ggaaattccc
    11041 tcaactgttt tttgaaagga ttgatttatt cagggaagaa tgctgatttc acaacttctt
    11101 tataaacggt gttcactcag ctgtccctcg gtattagctc aaaaaacggc aaaatataag
    11161 aaactatttt tttcgttttt ttctctaata ctcaggccag aacactgatt tcacaacctc
    11221 tttataaacg gtgttcactc agctgtccct tggtattagc tcaaaaaaag gcaaaatatg
    11281 aggaactatt ttttcgtttt tttctctaat actcaggcca gaacactgat ttcacaacct
    11341 ctttatttca cattcaatat ccacatgaat cccatgaata caaagaacag accatacatg
    11401 atgggagata cttcttttcc tcttttagct gctaacatgg taattggata gaagataaaa
    11461 cctaatgcaa tcccatctga aatactataa gtcaaaggca tgccgatcaa gatcaagaat
    11521 gaagggatcg caatctctaa gtttccccat tcgatttgtc gcaaagattg tgccatcaat
    11581 actcctacca taatcaatgc tggtgcagtt acttgtgaag taacaactga tagtaatgga
    11641 gaaaagaaca agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat acctgaacgt
    11701 cctccaacgg cgataccagc tgatgattct acaaatgcac caacaggaga agtacctaat
    11761 aaggaacccg caagcatagc tgttgaatca gaagctaaag ctttccctac acgaggcatc
    11821 ttgttgtctt tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc tgctgtatca
    11881 aagaaagtca caagcaagaa tgtcagcact acgacccaca tttgaagaga attgatgtcc
    11941 cctacatgtt ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc ggcaacaatt
    12001 tcagaaggca ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc tgccatcccg
    12061 atgaagatgc cacctggtac acgccgaaca agcaagatag cagttacgac taatccaaaa
    12121 atcgtcaacc aagtagaacc aacgttgagt gatcctaatc ctactaatgt agattcattc
    12181 gaaacgatga tcccaccttc gctcagacca aggaaagcaa taaatagacc gataccgcca
    12241 gaaatcgcat atttcaaatc tgctgggatc gcatcaatga tcatttcccg cagcttgaag
    12301 attgtgatca aaataaaaat caaagaagca acgaatacgc cggctaatgc tgtttcccat
    12361 ggaatcccca ttcctaaaca aacagaataa gcaaaaaaag cattgatccc aagagcaggt
    12421 gccgttccaa ttggataacg ggctaatacc cccattaaaa tacagccgat cgcactggct
    12481 aatgcagtcg cagtaaacac tgctccttca tccattccag aagcacctaa tacagttgga
    12541 tttacaaata agatataagc cattgagata aatgttgtaa atcctgccag tacctcacgt
    12601 tttaccgtgg tgttcaattt ttctatttcg aagtaggaaa gtattttttc cttcatcttc
    12661 catcccccta aatccgaaca ttataaataa agtccgttaa aatttcgatt tattaataaa
    12721 acaacgtgtt ttatcatata ctaaaacaaa acataagtaa acaaaaaatt cggattttaa
    12781 gcaaaatccg aacgttccat ctgattaaat ccctttatct caaagttcat gaacattcac
    12841 tttggattac aagaatttct ttattgtgtt aataaagttt cttcattcta gtctcaaaaa
    12901 tcaatagtgt gatttgattt taccatttcg tttctgttct tctttgatga attcttttgt
    12961 atcgacagga ttcagattat ttagtccgcc aaaacctgta tcgaccattt ttccccagcc
    13021 ttttgtcttc ttttttcctg atttgagatt ctttgctcga agcttctcac tatgttttgc
    13081 tcgccgatac ttcaaataac cttctaatct tcccattaga tccactgttt ctcctccttt
    13141 ttattcaatc agacgactca gcttttccta taacaaaata attgccttca gggtccgaaa
    13201 aattgaaagt caagacgcca ttgttgtctg tgatttctcc tggttcttcc atgttctcgt
    13261 agatagtttc aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct aatacctctg
    13321 gtgaaaactt ctgcacaaat acttttggga atacgcctaa ttctaattct tttgagatgc
    13381 tgagtacaat actcttaaat tcttctggca attcgattgt ttcagaaatt tctgcaccaa
    13441 ttttttctac aaagaatcgg caaatcatct ctacgtcatt gacataaagc atcacacgtg
    13501 ttttttgtat catcgtttga cccttctttc tatttgattt tctttcctgt ctataaactg
    13561 attccattat atcacgaaag cgcaaacacc ttgtgtttga tactattcaa gaaaaataaa
    13621 gcaatagttt ctaggattgc tttcctccac aaactttttt tacaaaaaaa aaacggcaat
    13681 gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca tctttggtta
    13741 gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc aacgcttcga
    13801 ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc agcaccgctg
    13861 cttgtgtttc ttccgtcagc atcgttcttt gggcttcacg ccagttgaga cagcgacaga
    13921 tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca tcggaatctg
    13981 cacctaaagg caggaatgat tcgtcacctt gggcttttcc taaatgcaga tttcctgcga
    14041 tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata ctattataga
    14101 tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt ttcagtaaag
    14161 cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc tgacgccact
    14221 gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt gcttcttttg
    14281 ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg tggttatcga
    14341 taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag aaattggtat
    14401 ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct tgatgaatag
    14461 atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt tgataaatcc
    14521 ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga gccaaatggc
    14581 tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg taccgatacc
    14641 ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat gatcagccat
    14701 ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct cttgatcgat
    14761 actcactagg aaaaaatctg tttctttatc ttttagctct tctgtaagtt tttctagtgc
    14821 ataagcttgt gtcaagtaat cagctaaatg ctccggtaat tatcttttcc aaatgtatct
    14881 gtaaatgttt cgatactaat ttgttgtagt aagtctacat ctgaaggcgt catttttcga
    14941 atttcgtatt tattcacggc ttcttccttt caatagatcc ttttattgcc ttttttgaca
    15001 aaattccaat cttcgctaac attcttgcat actttattta aaagatattc caattgtttt
    15061 gcttcttcgt cagaaagacc atttaatgcg actgcattgg agtagttgtt ttctcgaatg
    15121 atgaaaggat agatttctgc tcctttttta gtcggataga gtttcttgat ttttttattt
    15181 tctttatctt ccaaacgttc gatcatccca ttggattcta atttcttgat tgctcgggca
    15241 gtcgttgtac gatctacttt gatcatttct gctagttttt cttggatgat tccaggattt
    15301 tcgcaaatac gtactaaata aagatactgt cctcttgtta aatcgacttc tttgaattcg
    15361 atattgctaa tagagtctag cgctcgggca atcattccga tgtctcgaag gatttcagcc
    15421 atttcatccc acctttattt tgtttgtaag actagtttac ctctttttgt tgcaaatgca
    15481 actattttta ataaaaaaat caagttcttt tttaataact agtaaagtat attcttttct
    15541 tcttttacga aaaagctcaa tcacaataga tctggttttt gttttctaaa ataagtaact
    15601 gtttcttcgt tgttcgcttt tctgatagct ttggcaactc ttctatacta aaaaatccca
    15661 tgttggaagt ttcattattc tcaataaact tggcctcttc accgtggatc gcacaagcaa
    15721 aaacaagttt ataatactga aaaagctgtg gaatatcttt ttgtttattc gtatcaaaaa
    15781 ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc ttcatagact tctttacgca
    15841 cattttcttc aggagacaat cctatttcag caaatccacc aggaagtgac cattctttcg
    15901 tccgtttatc ttcgattagt aggaccttgc catctttttt gatataggca cgaacatcta
    15961 cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc ttttatttct tcgaaagttt
    16021 cttttcctat ttcactgatc agttccaatg aaatcgtccg gatctcctca aaacgctctt
    16081 tatcaaatac atcttttcca taatatagac cagcttgaga aagtgcgagt agtcttttgt
    16141 attgagccaa cttcttgttc attctcttcc cttctttttt agcaaatgta tgactatgta
    16201 atcaagtata gaagactggt ttaattacgg caaataaata taatgaaatc tgcaataaat
    16261 aaaagaatgt taaaaaactc gagaacaaaa gacattcgac ttctctgggg ctgtgaataa
    16321 atggcgttca aaagcttgca tctgcggtaa taagccccat caaacgaaaa atatgacgag
    16381 ctattcttgt ctggctgttc ttattaattg aggcaggaca gctttttcac aacctcccca
    16441 ctaaacggtg ttctcctacc aactcctcgg tcacaagtca caatattcat caaacatgag
    16501 aagctgtttc tttgaaattg ctccttgtga ctcggagctg aacgacaggc tcacgacctc
    16561 tcggtaaacg gtgttcaaaa gccaattttt cgatattaag ccatttcttt gttacttatt
    16621 gtcattgatt tctttttcta atttatccaa tagtggagtg taatccactt gagtatagtc
    16681 tgttacatca aattcatata gtgttcctaa ctggaatttg tcatattttc ggtcggcgat
    16741 gatctttttg aatgcttctt tcgtaatatg attggtccct ttcgttcgat cttccaatac
    16801 gtcaccatag tgataatgat ccatgatata gctcaaatga cgatctgatt cacgatcacg
    16861 tttgtaccga cgttgccata acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg
    16921 atatccgtat ctatctgcta attcttggag tcgtgctttt tgcttatcac taaaaggata
    16981 ctcggacaca atcactttct ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa
    17041 aggataaact ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa acccgatgga
    17101 ttctgcatag tattccttga tttcatcgag cgtgatggca tacatatctg gaaatcgttc
    17161 ccgtagttta ttcataagat atgtttttcc tgtaccagga ctaccagcca gaagaatgaa
    17221 atactttttc atctcattta tttcctaagt taattggact ttccaagttc ataaacagtc
    17281 caatcttatg cttgacaatt tcttttactg ctttatttgc tggaatcaca ttatggcgaa
    17341 gagacttgtt gatttctgtt tcactaaagg tttctttagc tactttcgtc cagttgacta
    17401 aaagctccgt tccaacattg aatttgcgga tcccgttatt gatcaattca ggataatctt
    17461 cttctttcac acctgttcct ccatggataa ctagggggac atcaactact gcatcgattt
    17521 ctttcagcaa gggaatgttt acttctgttt ttgatttgaa ttgtccatga ttcgtaccga
    17581 tagcaatagc taatgcatcc acgcctgtag cttctacaaa tttcactgcg tcttccggtc
    17641 ttgtatatac cttgtcattt tcagcgacat ggattccttc ttctgtaccg ccgattgttc
    17701 caagttctcc ttctacggaa acaccgtgcg catgggcata ttctactact gcttttgtct
    17761 tcaagatatt ttctttgaaa ggtagatggg acccgtcata catgacagaa gtatatccgc
    17821 tatcgattgc ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgt aagactgcat
    17881 caaccatttc ttcttctgca atagctgtca ttacattgac taacacatgc atgccgatat
    17941 atttagcggt atcgacactt gtttgaataa tgattggcga acccatttct tttgctgcgc
    18001 ctagcatggc aggaagcatt tctaggttat gcgtattaaa agctccaact gtaaaattca
    18061 actcttctgc tgtttttgtt acttctttca atgttgtata catcttttat tgccacctta
    18121 tctttttatt tgtttccttt gatcgttact tgttttgcaa tttgtcgcat ctcatccatt
    18181 ttgcccatgt agtaatcgat cccttgatca tcaccagcac gagcagcatc tgcacgcttc
    18241 tggttataga ggctcatcag ttttttatac ccatctccga tcgtctgttt attttccaag
    18301 ctcttttcta aatattggat ggcttgatcg ttctcatgca attcagaaaa tttaagtcca
    18361 acttgttcat aaagttccgc taatttatgc ctatcttggg tagtggaaat ctcttgtttc
    18421 aattgtgtaa tctctgattg aatcttttcg gtcgcttctg gcgatagggc tttttcttct
    18481 tttgccagaa cttttttcgg ttctttttct tttttcttaa aaaatccaaa catcattttc
    18541 cctactttca attaaaacgc tttcaaaact gggcttaaga tccaggcata taagagtgct
    18601 gcggctactg tatctacatc ggttgctgta gcattgatga atcccatcga attaaagatc
    18661 gttactagca aagcgggcaa taatgtaata aagaaaccat gagcaatacc gccaatgatc
    18721 gctcctcggc gtccgccaac tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa
    18781 ttggttagca taccaggtaa aatcatcgct aagccgaaca tgggcaaagt aaacatggca
    18841 ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga cggcatttgg gctataagga
    18901 aagaaaactg gacaatctaa ggcagggatc gcatcaggaa ctaatttcat agctatccct
    18961 cgaaatgccg gcacgatttc acctaacagt agacgtacac cagctagcaa tatatacaca
    19021 cccactacga attggatggc ttgtaaaaag gcaaacatga tatagttttg atctcctgat
    19081 aattcagaag cgaatggttc gcctgcaaat aaaacagtga tgatatatag cgggaccatc
    19141 acgaccatga cagacaaata agtatcttgc agaaattcga aatgtgtcgg caacttgata
    19201 tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa gcttagcaac accggcttca
    19261 aataagtacc cgatcgtaca aaaatgacct aaagcaatat catttgatcc agtgatttta
    19321 cgaatgatcg gttgcgctac tgccggcata gcaatggcga aacaagctcc gataaaacca
    19381 ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc
    19441 gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga
    19501 gccaaaataa tattgaatac aaaaatggct aagaacgtca gtgcaatatc tctacctaaa
    19561 cccaaatcat tcattgcttg atcattgata gattcaatag aagggatgat cccttgcata
    19621 tgaaatcctt cagtaaagat tttcccgaaa taagttaaac ttccaacgat gatgctcgaa
    19681 ccggcactca atacttgaaa tcctaggagt gtttttaacg tgccagagat gacttgtcct
    19741 gctgatttct tctgcagtag tagacccaac atagcaatca atgcaatggt gatcgatgcc
    19801 tgagtcaaaa tattttcgat gataaaattg ataacgctca tgtttttacc ctcttttctt
    19861 tttgtattta gataactccc ttatcttgca gaactggggt gattttttct tcaatttcat
    19921 tttttgaaac aatatttttc aaatagatca tcgccgtttt aggatcaact gcgaactttt
    19981 cgaactgcga acgaaaattt tctgctgtga tgatcacatc tggtttcatg gaaactgcgg
    20041 aagaaatatc acaatggtcc agtttggctt ctactcctaa tttgttcaaa acatcctcag
    20101 cagacatctg tgctgcaaaa ctacttccta aacctgcgcc gcatacgaat aaaatgttca
    20161 atttgttcat ctttgttctc ccccgtatca tttattattt ctcaactact gaaaaaagca
    20221 gttgtttgaa atcttcgata tttgtgcatt caatcaatcg atccaacttc tcttcgttgt
    20281 tgatcaattc aattagttct ttcatgatgt tcagatggga gtaggagtct actgctgcca
    20341 aacaaaaaat aatctttact ggatccattt caggattacc gaaatcgatt ggttgctcaa
    20401 tcgtagctac gctgactcct aatttattga ccccatcttc tggtcgggca tgggctagtg
    20461 ccatgtgttt gccaatcacg atatacggtc cgtaatcatt cactgcacga atcatcgctt
    20521 cgacatagcg ctcttcgaca atgttttctt taaccaatgg tctagcagct agttcaatcg
    20581 attcctccca agtagtggct ttttctttga tcatgatatt gctgtccgtc aaaatatctt
    20641 ttaacatagg ctggatctcc cttttgttga tttctaaatt gtttgtttcg aataattttt
    20701 ctaacttgcc atagatgatt cgatctactc ggccaccact ttctttcatg acatctatta
    20761 tttgagtgaa taactccgtc gattcatgac tgttaggctc taaccgctgt agttcttcat
    20821 ttcttttcaa aaagtcttct ataaccaaga tattcgtctc agttaaaatc ggatcgacaa
    20881 ctaaaagcgg cttatttcga taattcaatt gaaaggtgct gaaaaccaga ttgacatcca
    20941 atttatctac taattcgatt tcttttgaac ttaaaactgc agtaatctcg atctgaggaa
    21001 acttttcttt cagattctct gctaacagat ttccagtagc catcccatgg ttacagacaa
    21061 cgactgattt ataaacataa ttcaagtctc gcttgattac actggcgatc gtcgaaaagt
    21121 ggatgacaag aaaagctatc tcgtcctcca gtatcttgcc accaattact tcttcgatcg
    21181 taggaacaaa atgagtgact gcgcggtaga ttgggaaata atttgttcgg atgttttctt
    21241 tcaatggatt catgacttgg atatgattct tgattcttac aatcagtgca gccatgtgct
    21301 tatagagatt ttcgaataag gcttcttcct ttagatggaa aggtacgtgt gtctcgttct
    21361 ctacaaattg gatcaattgg atcgttagta attgagcttg tacccactcg attgaattgg
    21421 agatatctct agcattgaac gtctcaatag tgaaacgaag ataatttctt tctacttgcg
    21481 ggacattgtc caattgaaat tcctcgatca tgtggtcaat aaatgcagaa aagctactgt
    21541 cttttatttc tgtatttttc caattaatgg cagagataaa ttcatgtttc ctgattcgct
    21601 ggatccagat caatgtaaaa agcaggattt gttttttata aatatcatct tcttgtttca
    21661 acaacttatt ggaatagatc tcctgtattt ttttgatttc ctccaaagga aaataatgga
    21721 gaaagatttt ttgagccggt gttaattgtg catcttgatt cataaagaag tcggtcattc
    21781 ccagattctg attgataaaa tcatagatca tcgtacggat agatcgttct gaacctttat
    21841 aaaccagtcc ttgctttcca taactgacga tctctatccc ataactgacc agtgttgctc
    21901 gtaaccgtct catatcttca tctaatgtac ttttagagat ttgaaattct tcttcttttt
    21961 tgtttagatg gatcgggatt cggctgtaag caacggctaa aactagatca aaaatacgtt
    22021 cgcgccgatt caaaaagccg tctttcacat cttttatttc agtttctaat cgagcttgct
    22081 tttccttttc cagcttcaac aaaaaacctt tgttgcgaat tgtacggatc tccggaagct
    22141 ttttactgct caaccagata ttcgcttcaa gaacttcctg acgaatcgtc cgctgactga
    22201 cattgaactc acttgctaga tgttttgctt ttactggata ttgggctgtc atgattctgt
    22261 gaagtatttc aattgttctt gttttcatag aaatgatctc cttctgattt caagtatatg
    22321 gttccgctta cataataaaa agtagatatt tctgccactt gaagtggaaa gaaagtaagc
    22381 aaagcaaaca aaaaataagc catatgcttt ttcatctcct caacgaaaaa gacatacgcc
    22441 tattattaca aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa gctgtcctgc
    22501 atcaagtaat aagaacaact aaactaaaat agtgcttcct attttttgtt tggttgttct
    22561 tattatcgca gatgcaagct tttaaacacc atttagtgga gaagctgtga cagagatgtt
    22621 cagccttgag cgataagaag cgattttgaa aaatcgcttc ccatatttta caaaatcgtg
    22681 ccttattgcc gataggctat ctctggaaca ccgtttattc agaaaaagga gctgcgacaa
    22741 ggcttttgtc acaactccgt tcataaatat ttcctgtcaa tccagcataa tcggttgaat
    22801 aaaatcggct tgccatcttt tttctttctc agaaaaatca aaataccata tctgagaaaa
    22861 atactgtcgt tgtgtcttac ttcctgagat caatcgccca tcttttcgat cgactgaata
    22921 atcacagcat ctgaaatcaa ttcggatggc aaaactattt tcgtggatct gtcgataatt
    22981 tcctacttcc tgaatagtta tttttttagt atgatttctt acaccgtctg ctttgttttt
    23041 ttgcagaact ttctgatgtt cggcaaacag cttgtctgta taacagcttc tggctttttc
    23101 aggagtttct tcttcccaag cggattggat cttcatgaat atcttttgga tttccaaaaa
    23161 ctgtgctttc ttttctttag ccgttcctga catgcgggac aatatctccg atttcagcct
    23221 tttcttttgt cttttattct tgacgataga aaaagcagaa acccctccta ctgacaaaac
    23281 agctaatgca agaaaccccg tttgctcata actagaagga cctgtacttg gaccataata
    23341 cccataagaa tctctgccgt aagaacgact gccaaaataa ctgcccccat atgaacgacc
    23401 gccaaatgaa cctctgcccg aacttcgacc gctagatgta cctccaccat gaccacccgc
    23461 gacagcgaat ccttgggtcg gcagagcaaa caaacatagg cccactaaca aaatgattgc
    23521 taaataattg attttttcat tgtcttcctc gttatctttt attacaaaaa actattttta
    23581 tcatagcctt gctaaaagga agagtcaata accagatgca taaactgttt ttttagcaat
    23641 tttgtcttat ttagtcattg agtaacatcg gacagatgta tgtgtcggca gtccataatt
    23701 cctctatctt cagcatgaac aagcttgcag acaagagatc tgcgcttcct ccaggagaga
    23761 tttggttttc ctgacaaaag tgcaacagtg ctgtaaatgg acgatttggt aaagcacttt
    23821 gactagcagc atgagcaagt ttttgcattt ccactaacgt tttctgatca ctgcgatgca
    23881 atacgcatgt gtcatctagc gaactcatca aaactaacaa catatgcatt tgtttctgta
    23941 catatgaatc attatctagc tccttagctg cttggattgc aagcctgata tgcggatacc
    24001 ctaatatcgc ttcttcatat gcaccattta cgccatattt ttgcttcacc ctatgaccat
    24061 gagtggtttt tttatgacga tattttgtat caggaaaagc agcaagcttg ctgacatcat
    24121 caagtatttt cgataaagaa aaaggctgtt ccatactgat tttttgtgca atgacactga
    24181 tcaatagccc cattgcccaa atcgctcctt tatgggtatt gatgccattt gtagcaatca
    24241 gcattgcctt ttccgctttt cgcccaatct cagcaattct ttctcttaac tcctgatcaa
    24301 tagcataatg ccatgctatt tgagccattt cttcaaaaaa aggagttagc gtcttagcag
    24361 acttatccaa aatgcgccga ccttctttta cggcttttgc gatcgtatgg aaattatcat
    24421 ctcctaaaac catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcga
    24481 ttcctatatc ggctcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga
    24541 cgatttctcc atttttctgc aaagctttca cgattcgcag tttgtgttct ggtgtcgttc
    24601 gagcaaagac gtcgactttt ggtacttgtc gaatcaactc ttcttcagac ataagatcca
    24661 aatcgatccc ttctaaaact ttagcagtat gttttaagcc aatttgttcc gcgatcgctt
    24721 ttgctgtatc tttgtgatcc cccgtaatca ttttcactga gattcctgct tgttggcatt
    24781 cctttacagc tttgactgca ctttcttttg gcggatcaat caacccagca atccctgcaa
    24841 aagttaattg ggttaaagac tcatgcgtga gttcctgttt cgaatctact tttttgtaag
    24901 caaatcctaa aacacgctga ccttttttcg caaacgctgt ggctgtttga ccccatgctt
    24961 cttattctga agaagaaaga gttgatttag ccaataatac ttctggcgca cctttgacgt
    25021 aaatcacccc tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagagctga
    25081 acggaatttt tgcttctatt tttttcaaag gcagatcggc tgtttcggtg tggcgaatca
    25141 gtgcaagttc tgtcggattg ccacttaaat tttctgtttt ctgttcttct ttcgtcttta
    25201 attcctgaca atttttcata ataaacagta cgcgagtttc ttcctttgct gctacatcca
    25261 caactgtcat ttcatttttt gtcaatgtcc ctgtcttatc tgaacagatc actgtcatag
    25321 aacctaatgt ttctacagaa ggcagtcctt tgatgatcgc cttttctttt gccatttcat
    25381 gtactccgac agataggatc atggtcaaca cagctggcaa tccttcgggt accattgaca
    25441 ccaccaaagc gatcatagct gaaagaagga tatgccaatc catgccatag cggaatgttg
    25501 taaaaacaac taagaacaag atcaaacaaa gaatgccttg aaaaatttgt ttgttcaatc
    25561 ggttgatctt cttgatcaga ggcgtcgttt gttgttcgac cgattgcagt gcttgattga
    25621 ttttaccgat ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt
    25681 gtacaagcgt tcctgaaaaa gccatatttt tttggtcacc gattgattct tctgctgcca
    25741 aaacggaatg atttttttca gatgcttctg attctcccgt caaaatcgat tcttctacta
    25801 gtagatcatg tacttcgatc aaccgaacat ctgctggaac gacgtctccg gcacgtaaga
    25861 caatgacatc tccttttact agttctttcg cagggatttt ttcttgcatc ccgttcgata
    25921 gtactatcgc ttcttgtccc atcagctgct tcaaaccatt caacgattct tctgcttttc
    25981 tttcttgcca ataactaacg agtccgttga cgatcactac gaataaaata atacttcctt
    26041 cgatgtattc gctcgaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca
    26101 gatcagtaaa gtgttttgct atttttttcc atcgttttgt ctgctgtctt gcttctatct
    26161 cattatatcc ttgatcagtc aggcgtttct gccgttcttc ttgcgacaag ccttctgttg
    26221 atgtttgata gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc
    26281 ctcctgttta attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat
    26341 ttcagggggg tctgaaataa ttcgttttgc agaatgtatc tgggcctaac tgatatttct
    26401 attgcttatt cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg
    26461 aataaagcgt gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa
    26521 caaagcaaga tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac
    26581 atgtgcacac aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa
    26641 aagctgaaaa aacaatcgac tatcagctac aaaaaaatgt agacaaagac cgacaaaaaa
    26701 taacatccct gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa
    26761 ccaatggggc aaacttttct cttttgctgt ttgatataaa atcctgctag tactgtagac
    26821 tgcactattt gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc
    26881 gctttggcaa tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca
    26941 atgccatgga acaacaaata aaatcgacaa aatcgttcct atatagaaaa agaggatacg
    27001 ccaaggcaga cagttgattg catttttcaa attcttttgt gggtcactcg tctcaccaac
    27061 tgttaatccg atcatttcaa ttccagcaaa actgaataaa accataggaa atgaagcaat
    27121 cattccagca aagccaaacg ggactaattc attgagctgt ccaaaatttg ctttgattcc
    27181 aatagactgg gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa
    27241 aatccccaag atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc
    27301 aacaaggttg actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac
    27361 atgtacaaac cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc
    27421 aagccagcaa agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata
    27481 gtcaacgaaa tcactgatag agttaaatgt actatttgct aaaaataaat cacccatgac
    27541 ccgcatcaaa ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc
    27601 tgctgatgca attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa
    27661 tgcgattaac tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa
    27721 gttcaccttt ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta
    27781 ttttttcaat acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta
    27841 gattgatcca tgtcccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata
    27901 aaacaataac gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa
    27961 atggtcgatt caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga
    28021 catagacgat acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga
    28081 cgccaggaat cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa
    28141 taacggattt ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg
    28201 caccttttct agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt
    28261 ggactgctac accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac
    28321 caagaatttg gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt
    28381 ttggtacgac atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta
    28441 acgcggcaaa tactccgcga atatacgttt taactggatt tttcaaccga gtgatataca
    28501 cagaggacat ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg
    28561 atgcagtcgt agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg
    28621 gtacgatccc aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca
    28681 cgtacaatcc taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat
    28741 tcaaaaaagt gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca
    28801 aaccaagcgg cacattatcg ataatattac caaacaacgg agcaagtgct gaagcgacca
    28861 tcaccatccc aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt
    28921 ttcctagagc attcgatacc caaagttctg gaccaccttt ttggggaaac attccggcaa
    28981 attcaccaga gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa
    29041 aaatcatcgt ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg
    29101 cgcaggtcat tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca
    29161 tctttattcc ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga
    29221 acagaaatga caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt
    29281 gaacgatctt agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt
    29341 ttataaaaaa gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt
    29401 tgatgaagca aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat
    29461 cagttctatt ggatgatttc gtttagtttc ttttccattg ctttggcgat ttcttttgta
    29521 taaaaattga aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga
    29581 gtgattttct gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga
    29641 atcggagaat caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca
    29701 ccataaacat cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg
    29761 atagcgttca aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca
    29821 ggatgattca atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg
    29881 agaaattctc tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg
    29941 gtcacattca atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct
    30001 tttgatcctt ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac
    30061 acatatggcg cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct
    30121 ccagcagcat aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct
    30181 gaaatagctc tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca
    30241 tgatgttctt caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt
    30301 agaaacagtg aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc
    30361 ccttcatctt ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct
    30421 tcttctgtcg ttcctacgac agcaaccaca cctagaattg ggattttttg ttcagctaat
    30481 tcacgaattg ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata
    30541 gcaaccatct gatctaagcc tactccgcaa atatctaacg ccttcatcca agaatagtgt
    30601 tttgtttgag gcaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa
    30661 gctgctttga cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct
    30721 tcaacagata aattcaaaag ttcccactca gacatttttt tcacttgttc aggcagcact
    30781 tccttgacag ctaaaggaat cgatttgatg cagcgggcgt accataatcc ttcaagatta
    30841 gcaatagaac catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca
    30901 aaatcctgtc ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca
    30961 ttatttgggt tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca
    31021 gcattcattt gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat
    31081 cgctgactga cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga
    31141 gtgagcttat ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat
    31201 ccaagatgtt catctaccat cttgttcaac aacattttat aaactggtcc attttcagct
    31261 ttgtcgccta taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt
    31321 tgttggtcaa tgtctcgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc
    31381 aaaaatgaga aatttttttt tataacatca aatccacgta gattttta
//
LOCUS       DACDNO010000031        30131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDNO010000031 DACDNO010000000
VERSION     DACDNO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.031"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(428..1372)
                     /locus_tag="IWI22_001476"
     CDS             complement(428..1372)
                     /locus_tag="IWI22_001476"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9168872.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1668..2612)
                     /locus_tag="IWI22_001477"
     CDS             complement(1668..2612)
                     /locus_tag="IWI22_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9168873.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2833..4047
                     /locus_tag="IWI22_001478"
     CDS             2833..4047
                     /locus_tag="IWI22_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9168874.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4069..6084
                     /locus_tag="IWI22_001479"
     CDS             4069..6084
                     /locus_tag="IWI22_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9168875.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6167..7540)
                     /locus_tag="IWI22_001480"
     CDS             complement(6167..7540)
                     /locus_tag="IWI22_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9168876.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7566..7850)
                     /locus_tag="IWI22_001481"
     CDS             complement(7566..7850)
                     /locus_tag="IWI22_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9168877.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7852..9867)
                     /locus_tag="IWI22_001482"
     CDS             complement(7852..9867)
                     /locus_tag="IWI22_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9168878.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKTLLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10046..10351)
                     /locus_tag="IWI22_001483"
     CDS             complement(10046..10351)
                     /locus_tag="IWI22_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9168879.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10440..11471)
                     /locus_tag="IWI22_001484"
     CDS             complement(10440..11471)
                     /locus_tag="IWI22_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9168880.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11678..12553
                     /locus_tag="IWI22_001485"
     CDS             11678..12553
                     /locus_tag="IWI22_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9168881.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12705..13547)
                     /locus_tag="IWI22_001486"
     CDS             complement(12705..13547)
                     /locus_tag="IWI22_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9168882.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13639..15516)
                     /locus_tag="IWI22_001487"
     CDS             complement(13639..15516)
                     /locus_tag="IWI22_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9168883.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15513..16934)
                     /locus_tag="IWI22_001488"
     CDS             complement(15513..16934)
                     /locus_tag="IWI22_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9168884.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16937..20326)
                     /locus_tag="IWI22_001489"
     CDS             complement(16937..20326)
                     /locus_tag="IWI22_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9168885.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNEVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20583..21974
                     /locus_tag="IWI22_001490"
     CDS             20583..21974
                     /locus_tag="IWI22_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9168886.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNGRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22047..22694)
                     /locus_tag="IWI22_001491"
     CDS             complement(22047..22694)
                     /locus_tag="IWI22_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9168887.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22702..23388)
                     /locus_tag="IWI22_001492"
     CDS             complement(22702..23388)
                     /locus_tag="IWI22_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9168888.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23394..24467)
                     /locus_tag="IWI22_001493"
     CDS             complement(23394..24467)
                     /locus_tag="IWI22_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9168889.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24689..25363
                     /locus_tag="IWI22_001494"
     CDS             24689..25363
                     /locus_tag="IWI22_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9168890.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25368..26708
                     /locus_tag="IWI22_001495"
     CDS             25368..26708
                     /locus_tag="IWI22_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9168891.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26857..27843)
                     /locus_tag="IWI22_001496"
     CDS             complement(26857..27843)
                     /locus_tag="IWI22_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9168892.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28567..29598
                     /locus_tag="IWI22_001497"
     CDS             28567..29598
                     /locus_tag="IWI22_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9168893.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29707..>30131
                     /locus_tag="IWI22_001498"
     CDS             29707..>30131
                     /locus_tag="IWI22_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9168894.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT         8936 a         5640 c         5406 g        10149 t
ORIGIN      
        1 tttaaaaaat gataaacctc tctcgaacgg ttttttatca aaaatcgtca tttttccatc
       61 aaaatctgct tatgcaaaat tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt
      121 ttaattgaat gataaaaacc agtttcttta ttcagttaat caaatatgaa cgaattagat
      181 atgaatcatt gtatttgccg ttgcggaagg attttttaat taaccacaga taaacattga
      241 taacgattcg cttattgtaa tcaacagatt aaatttcata tgttcatatg tacggataca
      301 ttttcggacc gattaatttt aaccattctg tttatttgct tacctattag atatcaataa
      361 aaaactgctt aacagctatt tcagctgtta agcagtttta ggatttttag ttcatattca
      421 ttgtttctta taatacagtg ttcaatactt ctcttaaagc acttgttgat ttgctgaaca
      481 ttgctttttc ttcttcgttg attgataatt caacaacttc acgaacacca ttttcgtcaa
      541 cgattgatgg cacacctgta aagatatctt tttcgtcgta ttcaccagtt aaataagcag
      601 atacaggcaa tacagcttgt tcgttattca agatagcttt tacgatacgt gcagtactca
      661 taccaatacc atagtaagtt gcttttttac gatcaatgat ctcataagca gcattacgga
      721 ctttatcagc aatcacatct aattcatctt gggcaatacg atggtctttt tctacaattt
      781 caaacactgg tttaccaccg acagttgtat gtgaccatgc agcaacttct gaatcaccat
      841 gttcacctaa gatatagccg tggacgctgc gtgggtcaac ttttaatttc aatgcgattt
      901 ctttacggaa acgagttgta tccaatgttg ttccagtacc gataacacgt gatgttggca
      961 gaccagattc gttccaagcg atatatgtca aaatatctac tgggtttgaa gcaacaacga
     1021 taataccgtc gaatcctgat cccatgattt cttttacgat ttggcgcatg attgacgcat
     1081 ttgttttaac taaatctaaa cgagattgac caggtttttg gttgatacca gctgtgataa
     1141 caacgatatc agcatcttta cattcttcat agccacctga ccatacagcc acattttcat
     1201 cgccccaagc cataccatct aaaaggtcca atgcttcccc ttctgctttt tcttgattca
     1261 catcgatcaa tactaattca ttagagattc cttggttgat cattgcataa gcgatacttg
     1321 ttccaacaaa acctgtaccg acgattacta ctttacgact tgtttttttc atgaataaac
     1381 agctcctttt cataactatt tgttactttc atgaaaagta tagtcttgtt tattcatttt
     1441 atcaacatgt taattataat agttgttatt ataatttttg atggaaacga attcctgaat
     1501 acgcataaca gataaatgac aaatgtttac acctatatcc cctgataata caaccaaaag
     1561 tttagaactg aaaataaatc caaaaaaatt cgctgcaacg atataataag acagcaatcg
     1621 cacctaaaac aattttcctt catatgcttt agtgaaagat tttcttctta atgtttattt
     1681 ctccattcat tgggagtaca tgttgaatac ttcttgaata atttgaataa agaagtcgtt
     1741 tgtttatatc cgatacgatt agcgatttct tccaacgaaa aactcgtttc ttgaatcagt
     1801 tccaacgcaa agtcgaattt tttctttaga accagttctt gaaaggaagc atcaaaatac
     1861 attttcaatt tattactaag atagaccgga ttgtagccaa aatgagctgc tgtttctttt
     1921 aacgtacaag aagtaaaatg cttatcgata taatctaata ctctaagcat ctcattttct
     1981 gcgcatccat ctgctgatga aaagctttgt tgttctaata aatgagtcaa ttcgatcagt
     2041 aatagtgatg tatagagatt taacgaacgg atcttatgac tgtatgtatc aaaataaatc
     2101 aacagcatat tttcaatcaa atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg
     2161 aattgtgtat gttccccttt ttcttttgat gcatttatca aaaaagaaga aagtagatta
     2221 tttgtgcttg ccaaatgatt caatatctct gtagataagg tttcttcttt taataagatg
     2281 ttgattaaaa tatcctcttg ccctgtgtat tcgattcttt gagtagcttc tttatcaatg
     2341 atcaccacgt cactttcttc catctctatc ctttctccat taataatttg taaacatgta
     2401 ccgctataca tataattgat ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat
     2461 cgacggtgtt tactgatatg aatatttccc tgttgaaata gatcccctcg gtggattttt
     2521 ggcggtgtct gaagatggta agctaagttt aaatcattga tattcattcc ttgttcacgc
     2581 tgcagagttt cgattgattc ttcgttacgc aaaaaaaatg tctaattcag ctagttccaa
     2641 ttgactccct cctttttgta agcggtttct ttcgcttatt agttcacgct gtactgctat
     2701 tatatgcaaa gctatttttg gtttcataaa gactttctat ggttgggaca tgaacttcct
     2761 gtgattccgc tattgagaga ataaccactt catactatat ttttgataag cgcttacaaa
     2821 aatgaaagga acatgcttat gaataaaaag atgatgaaat tttctctgct gtcagtctct
     2881 cttattttga cttctgctgg tgcagtcacg tctgcaattc ccagtatgtc agctacttat
     2941 gccacgcgtt ctttatctgc tgttgaatta ttaacgactg ctccctcttt tatggtcact
     3001 atttttgttt tgattagtag ttttattgct aggaaaatcg gacaaaaaca aactgtgatc
     3061 atcggattag tgattgcatc gatttgtggt atcctccccc tcttctcaca aagttacagt
     3121 attgtcttag cttcaagagc tggactaggt attggctttg gattgatcaa ttctttggca
     3181 gtcagcatga ttgctgcctt ttatcacgga gatgaacgag cctctatgat tggttttcaa
     3241 agtgcatttc aaggatttgg tgcagcttta atgacatttg ttgcaggaca gctagtacag
     3301 ttcggttggc aatatacctt tttagtctac gccatcagct tacctattct tgtcttgttt
     3361 gttttgtttg ttcctacacc tgaaacaact gaacttcaat cgcatggcac agccccaaca
     3421 agacaagtaa tcaaaacgaa tcctaaaatg attagttatg gtatttttct cttgctggtc
     3481 atcgtggtat ataatgctgt atcgattaaa ttagccacag tcctgacgac ttattctcta
     3541 ggaaacgaaa ctaatgcagc gaatatctta acaatcatgc aattggcaag tatggctgcc
     3601 ggagtttgtt ttggctggct tcagacaaaa gcacatcgct atatgcttac actttcttta
     3661 ctactgatgg gaatcggctt tgtattgatt gccactgtga taaacctgta tgtgattggg
     3721 tttggtgcga tcctaacagg aatttctttt tcgttattca ttccttactt attcaaccaa
     3781 gtaagtatcc aagcgcccaa gtacgcggag agtttcaata cttcaatctt attggttggc
     3841 gctaatttag gtactttcat tgcaccatat ggtttgctct ttctctcttt tgcttctctt
     3901 gggagtaaaa cacttgctgt tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt
     3961 attagccttc tagtagtaca caggacgaat agaaagacag tgataagagt agatgaagaa
     4021 ataaaagaac aattgacaac aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa
     4081 aaacataaag atagatggct ttggtatcct ggagactttg agatccgcca tggactgcta
     4141 caaaatttcc aacgggaaga acgaggtttt gactggccag cgtactggta catggatgat
     4201 tgccatcgga acgtcaagtt taagagatat tactttcttg atcaaccttc catgtttaaa
     4261 gtgacgatcc aaggagttgg gtatgtagaa attaatggac aaaaacatcc atgtggaaaa
     4321 tggctgactt gtccagctgg taaagcaaaa attcgtatct ttgtaggaca tacatcaggg
     4381 ctaccagcga tgtttatcga gggagacgaa gtaaaatcag atattggctg gacagcaagt
     4441 aattttattg aagaatatcc agctggctgg agtccgcttt atgtcgatat cgcaaaagat
     4501 ccaaatcaaa tctattatca aaaagaatgc gtccatccag tcactgaaac ggagattggc
     4561 ggaggaatct tattcgattt tgggcgagca gtgaatggct gtgtagaact aatatctctg
     4621 gatgaccata tcgaatctat cactctatgt tatggagagt ccgatgtaga agctatggat
     4681 atcgattatt gctactataa gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc
     4741 gcttttcgct atttgtttat accagaggtt tctactggaa tggttcaact tcaagttttt
     4801 catgaatttt tatctctttc gaaacaagct gctttttcaa gcgattcttc tttactgaat
     4861 cagatctggt cagtatcaga agaagccttc aagttatgta gtgatttgtt ttttatcgac
     4921 ggtatcaaac gagatcgctg gatttggtct ggagatgcgt atcaatcatt catgatcaat
     4981 caatatcttt tttttgacga ggaaatcaat aaacgaacga tattagcatt aagaggacaa
     5041 aatgaaatca agcagcatat gaatactatc gttgactatt cgatcttgtg gttgattggt
     5101 atcgaaaacc actatatgat gacgaaagac tatgaatttt tacgcatcgt ctaccctaag
     5161 atgcaatcat taatgagata cttgatggag caaacgaatg aattagggtt tatttatggg
     5221 agagcaaaag attggatatt tattgattgg tctgaaatgg ataaagaagg aacgcttgct
     5281 gcagaacaag tcttattact gaaagcctat cattccatga tcacttgcgg agatatctta
     5341 gctgaaacat atggagagaa cgaaaattat catactcatc tctatcaaga aaaatatcaa
     5401 ctattgaaac aaaacctatt tgactacttt tgggatgaag aacttggcgc ttttatcgat
     5461 tgctatgaat ctgggagaag aaatgtgacg cgtcatgcga atattttcgc tgttttattc
     5521 gattttgcta acaaagacca acagcaatcg atcttaaaga atgtcttatt gaatgacact
     5581 atcacacaga ttactactcc atatttcaaa ttctatgaac aagatgcttt atgcaaactc
     5641 ggacagacga atatcgtcta ccaaacgatt cttgattatt ggggagggat gttatctcat
     5701 gatgcggtaa ctttctggga agaatacgac cctactcaaa caggaaatga aaagtacagt
     5761 atgtatggag atccttatgg aaaaagctta tgccatgcat ggggagcaag cccaatctat
     5821 cttttaggac gctattttat cggtttgtat ccaacaaagc caggttatga agaatttatg
     5881 atacaaccta atctagattc atttgaaaca ctgaattgta cgctacctat caaagatgga
     5941 aagatttcgc ttgaaaaaac aaaagataag atcgtgatta cttctgatag aacaggcgga
     6001 acagtgaaaa tgaatggtaa aagttatcct cttctagcaa aacagccact tgtcttaaca
     6061 agctattcaa aaataaatgc ataaaacaaa aaactgcttg cgacatatca gaagacttta
     6121 aatcttcttc tgatattgtc caagcaattt ttattttgaa gtttgcttaa ttctgttctt
     6181 tagaaacaat gtttgttctt tttccggcaa aaagaaagct agataatacg acaagtaaaa
     6241 agaaagcgat gattccaact gtcaaggcta tttgaccgta ttctcctagt ttcccaagta
     6301 caagtcctgg tactagataa tcagtatcgg caaatgtaga agaagcgatt ttaagatctc
     6361 ctaatactgg aaggataaat aatggcagcc aactaatcaa taagctattg aaagcagaac
     6421 ctaataccgc ccctcttcta ccgccagtcg agttgccgaa gacaccagaa gcagctccgc
     6481 agaagaaatg tcctactacc ccaggaatga taactgtagt ttgcaagcct atcatagcaa
     6541 acatggatag tgttcctaca aagaagctaa caaaaaagcc aattaataca gcattaggtg
     6601 catatggaaa aacgatagga acatctaatg ctggtttaga atcgggtacg agttttttag
     6661 caatcccttg gaatgctgga acgatttctg ccaacaccat tcgaacccct tgaaggacga
     6721 taacaaaccc agctgtgaat gttccagctt ggatcagtgc aaagattaca gcgttgtctc
     6781 cattgcttag ttctctttca acaaatccag gaccagctaa caaagcaagt gcgacatata
     6841 cgatcgacat caagagcatg atcgatacag tagaatctcg caaaaagctg aaacttttag
     6901 gaatctttat gtcctctgta gaacggtctt tgtttccaaa aaactttccg atccatccac
     6961 tagaagcata accgatattt ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt
     7021 tcttcataaa cggttgacaa agagcaggcg taatagttaa tagtgttcct tcgaagaccc
     7081 cgcctaataa gatcatccac aaatttgcag taaaccccgc gcttactaaa atgactgctg
     7141 ttagacaaga aacataaagc attgcttgac ctgtaagaaa aatgaattta tatcttgtga
     7201 atctagcaag taaaatattc acgatcatcc ctactagcat gatcaaagca gtaggtgtac
     7261 cgtattgttg caaagcaatc gccactactg cttcattgct aggaactact ccttgagtat
     7321 gcagagctgt ttggaacatc gaagcaaaag gggttagtga attactgatt aaaccagcac
     7381 cgccgatcag tactaggaat ccgacaaatg ttttgatagt tccttggatc acttctgaaa
     7441 atttcttctt ctgaagaacg agaccaagca gtgcaatcaa agaaacgagg atggagggct
     7501 gactgacgat tgacacaaac aaattcaaca atttatccat tgatagttcc tcccttgaat
     7561 catttttatt cttttgacaa gatcttcgtt actttttctt ttacttcttc gctgtcaatg
     7621 atcgattgca aaggtacgag cttttcatca ggtatagaag ttagcgtatc tttcaaagaa
     7681 tgctcaatga agaaataatc agccatatcg ctagatacac ttccaacatc cgtatgactt
     7741 acttcaaact cgtccacatc tgctccaaga tctgcaagtg cttgtttgat gttcatctct
     7801 accataaaac ttgttcctaa tccactttga catactgcaa aaattttcat atcattcgtc
     7861 tccttttctt atgatcgcta atatttcttc ttttgttgat gcgtgtaata aatcttgaag
     7921 attttgttta ttagaaagta gctttgttaa atttgccaaa gcccgaagat gtgcctcatt
     7981 atcgatagca gctaaacaga tgaacaaact aatgggatgg gctggattat caagcagatc
     8041 cacaggttgt tgaagtttca gcaaagacat ccctacttcg tttacgccat cttctggacg
     8101 agcatgaggt aatgcgatgt gatcgccaat atggataaac gcaccatact gctccacgcg
     8161 ctcgatcata gctttgatat aactttcttc tattttattt tctgataata gcggctgtgc
     8221 ggcaaatgtg attgcttctt gccagttttt tggttgatcc gataattgga tcatttcttc
     8281 tgttaataat tcagatagca taggacgttg atcctccttc ttttctaaaa gtttgttcat
     8341 tttacgattt aatacttgat agagcttttc ttcgtctacg ccttctttta attcgatata
     8401 aggcagtagc gccgacaaca attcttgggc agaaggcatt gaaaatcctg gtatcagcca
     8461 atctctttgt acttgattga tcagctgatt tttttccaag tcagtcatga ttggatgcag
     8521 aacatagaga gactttttct tatcagtaag tgtgagaggt acagtagaaa aaataatatc
     8581 gtaagaagtt tcgtcaacct cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat
     8641 catcgggaat aattgctgaa gttcactttt taagatcaat gaagagctaa tgccacttgg
     8701 acagacgatc acggctctta tttttctatt tctttcttct gcgtccgctt ttctgatctc
     8761 tccgccaaat agtatagtga aaaaaccaat ctcttcttca gggatgctct tccctattcg
     8821 tctttctaac ggaagtaatg tttttcttgt catttcgtag atggaagcat attgatgctt
     8881 aatttgatcg atcatcacat taggtaaata aaaagaatag gaaatacgaa aataggcagg
     8941 gaccaagtga taaaagacat tcatcaataa ttcacgaaac gaagcaaatt ctacggccat
     9001 caggaattcc atgcgatgga tgacttctga agaacattcc agcaaaaaat caagtgcagg
     9061 ctcctggaca tctccttcaa cgatcgatgc taagcaaaga gagaaaaata taatctccga
     9121 attttgatgt tcggaatagc caaggccagt caatacttgt cgactaatgt cagtgactgc
     9181 aagagaagcc aacagttcag catcttttac cagttccatg gatgtttttc caattcgaat
     9241 ggttgtcaaa ctcaaataat aagctaattc atccattcga tttggtgcga atcgaagatc
     9301 tgatagtgaa atcgttttat ctagcaaatc tctaactttg gcgtaataat caatggaaag
     9361 ggttgcggtt aaatacgtga gcccaaatct tccagcaagt gactgcatca aacgagcagt
     9421 gcgatttttt gcttctcttc ttaatattgc ttctgaccct agcagcctaa aaccagtttt
     9481 cctagtatac gacagctgac aatcttccac ttctaattca tttcttatct ttttgatatc
     9541 tgacaggact gtccctttag aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga
     9601 cgttgcagtc gaataaacca tcaaataaag aagatctctt cgctcttgtt cggtatacac
     9661 agcttcaaat cgagtaatat ccaaaaattt ttctgcccag tcgattgctc ggaatttctt
     9721 tatacggata cgggtatcat ccacatattc ggcaaaagga gttaattgtt tgatcgcctc
     9781 gcttgtattt acttcgatga tctctgctgc tttttctaga ctgatttctc ttggtgtttt
     9841 caggagataa ctaattttgt agttcacagc gctgttcacc tcctacttat attctagtat
     9901 ggtagcgctt ttaaatcgag gggtgtttgg tacaacttac agttggattt tttttacgac
     9961 tgtaccgaac gatttctatc gaatggatac agaaaaaaag caggtgacca aatgtctcct
    10021 gcttttatga attgatttcg tccgatcaat gatgatcatg tttatttttt gaggtcagtt
    10081 cttcaaattt ttctttgaca tcttcaaccg ctttttcgat atctttttca aagtgattgg
    10141 gctcattagg ctttgtttcc tcttctacag aatttgaacc agataaagct gtttctttat
    10201 tttctgtttc accagagttt gtgccagcta atgattcttc tttgtcttct gtttcaccag
    10261 aattagttcc tgctaaagcc acttcttctt ctgttttagg ggccattgaa cctcttaatc
    10321 gtttgtgttt ttcaccgtca taatttgtca tgttggttcc tcctagtagt tctatacttt
    10381 ttcatcgtac aataagacag tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt
    10441 catatttttc cttctgattc ttgaatgctt ttgattcctt cttccaaaat tttcatttgt
    10501 aagattgttt cttcatcttt gatgactttt tctgcgccat taactaatgt ctcgtaaatc
    10561 ccttcgtaga cacggctgta atcaccaacc tcggaaatta ctttttcttc atgataactc
    10621 ccttgttcat ctatataggt caatacacca taatgttcgg gcaaatcgat accaaagtct
    10681 tcatgatcag gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt tgtttgtttc
    10741 acgaacatcc ctttttttcc gtaaacgacg aaacttggac gttctttgat gcggaaataa
    10801 ctggatttta tcgaaatttt catatttcca tagaaaaaat ccagatcgaa gtaatcattc
    10861 attcgtcctt tccctaaaag ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa
    10921 taagaaagaa cttgatctac tgtatgacaa gcatgtccat ataaatagct gttcgcagta
    10981 gaaaattcat gcgtattttc tggtacttcc ggtctgaaat aatcataatg catttctact
    11041 tctaaaatat cccctaattt tccgctttcg attacttttt ggactgtcaa gaagtcagaa
    11101 tcgaaccgtc tgttttggta acattggata aatagacctt tttcttttgc taatgcaaat
    11161 aactcttctg cttcctttga cgtttctgca aaaggtttct ctactagtac attcttacca
    11221 tgttcgagtg ccatttttcc atattcataa tgaaattgac taggtgtagt gatgacaact
    11281 aattggatct caggatcatc ccaaagatca gacagctgat cagtatattc gatgtcttcg
    11341 aatgttttcc acactggttc agatggggaa aaaatttttt tgatattgat tttgttcgta
    11401 agttttcttg aaaaaggcaa atgatagcga tttgtactct ttccattgcc aagataagcg
    11461 attgtaagca tgagtggtgc ctccttttat tgatgttttc agtatatcct agaaaagcgc
    11521 agaaagtatt tataatagct attctggtaa aaaagttcag aaaaacactt gttatcattc
    11581 ggtttggtga aattaatgtt caaagtaagg ataaaaatca aaaagatagt agcggaacca
    11641 agtgtttatc agtaagatag aatttggagg tcagatcatg cttattgaag aaaaactaac
    11701 aaaacaagag ttgtttacca cgacagagaa acggattgtc gattacatcc gtaggaatat
    11761 cgaagctgca gtctacatga cgattgaaga attagcaaaa gctacgtata cttcccattc
    11821 agctatcatt cgtttatgca aaaaaatggg attcagcgga tttaaagaat tccgtttcga
    11881 gctggggcgg gaagttcatc aattgattgc tcagttcaat caaacgattg atcctaattt
    11941 tcctttttca agtgaagata gcactcagca aattgcaaag aaaatggcgg atttatcgat
    12001 ccagtcaata aaaaaagcac aattgcagat tgaacatgaa gatttagatc aaatcggtaa
    12061 agtattgaac aaggcacagc gaatctttct ttttgcaaaa ggtgattctc aaatcacggc
    12121 aaggaaattc cagaataaaa tggtcaagtt gaataagttt ttgatcatgg ctgaagaata
    12181 cagtgattct tcttggaatg cagccaattt gactagcgag gattgcgcca tttttattag
    12241 ctacagcggt cgcatccatc attatgaacg aataatgacc tatttgaagc atgtagaggc
    12301 ccctaccttg ctgattaccg gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg
    12361 tcttgttata tcgcaggagg aatatgattt tgctaaagta gcgactttct cctctcaaat
    12421 tgccttcgat tacgtattaa atacgttatt ctcagtgata tattctcaaa attttgaaga
    12481 gaatatccgc aacttgaaaa acaaacaaga attgatgcaa aaaggccttc ttaaagaata
    12541 tcggaaaact taagaaaaga tcctcattta gtaataagaa agatagtgtt tgataggctc
    12601 tctttgatat catcataaaa acaggctgaa gttttctttt tcaaacctca gcctgtttgt
    12661 ttacggaacg attcgaatag atatattata gaaatgagga atagctaact tctgtttctt
    12721 attttgaacg attgacgaaa aagatatata agaaggagga aaatcagtat acatgcagct
    12781 aacaatccat acaggaaaaa tcgtttgttc tcggatactt tctgtacttc tttttgaact
    12841 tcttttgcat gataaggaac ccggtgccct ctgacaagta agcgatgact gttgaccata
    12901 taaggtgtac aagttaggag tgtcacgaaa tcctgccctt tttcgattct cagatctttt
    12961 gtatcagtcg gttctaccgt cttgatctga tcgacttgat aagcgagtgt ccgtccattg
    13021 atttcgatat agaaagaatc acctaaagtc agtttcggta agtctgtgaa caactttgct
    13081 tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat tttctccccc cgtaggataa
    13141 gacgttcctt ccaataatga ggcacctttt tctagcagca cagtattcgt ttcatcaaaa
    13201 attggcaaat ggacattgat ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa
    13261 taggttctat cctttttcgc aggttctttt ttcttagtaa agggatctgc acctggattt
    13321 ccaccttctt ttgctaattg ctggtttttc tttgtcatgt tttcttggat tttagccatg
    13381 acttcttcat tttctttgac cgcttgttgc tgataatgag agatgatctg ctgatccaga
    13441 taattattca gcgcgtcgct aacaaatgga taagctagta tccctattcc tgagagaagc
    13501 aagaaaagca tcagcagatc tagaagtttc cttttctttg gtttcatttg tgttctcctt
    13561 gtgatgactg tttgataata aaagaaaaag gagcatttgc gtaatccgct gctccttttt
    13621 ccaattcttg caaaaagact aaatctgact gtgcttgcgt ctagcaaagt acagtccagc
    13681 aatcagtaga aggactactc ctgcaccgat atacacatag attcccttac cgcctgttga
    13741 aggaagtgta cctttgtgtt tatttggtat tttttcagga gaaatcaact gtcctgctgt
    13801 tacataagat tgttcatcga tagtaaatgc tacacggttt gttaatggaa catatttttc
    13861 tggcgctttc gtttcttcca gatagtacgt gccatatttt agacctgtca catcgattaa
    13921 accgttactt gtggttgtaa aaaccgttgc tgattctttc gccgataccc agctgacggc
    13981 ttttgtggat gggtcgatcg ataaatattt cgctgtgtca ctatcttgat cacgaacgac
    14041 gaattctgct ccagcaagtg tttggtctga tgtaacgtca ccatctactt taacaaatcg
    14101 tttgccccca gtaacgacat cgactgacgg tggtgtttga tcatttgtat gcccgttatc
    14161 gacatttgct tggttagaaa atcctttggt tggatctgct ttttcgttca aatgcatata
    14221 gtaaacgaat ttcaatgtac cgccaggagt taatgaagga atataattcg gatcaactga
    14281 aaccgtgaat ccgtctgttt gctcagtgac agaataattt actgggtcga tttctttatt
    14341 tccatcatat aaggcataag catacgttcc tgaagaatca ttatcaaatg ttaaagcagc
    14401 gtcatgagta tcgataagtt tgaatgttgt gtacttgttt tgcgttcctt ctttatcagc
    14461 gatacctaat gggatattga cagaaatctc atattgaatt ttttcaccga ttgcgacatc
    14521 ttttccattc aattgaggtg ttgttttatc aactttagac gtatcatttt tgatggtctt
    14581 ttctactggt gtttggctat ttgccaggat ctcaaaaggc gtgattgttt gcttttcaat
    14641 catttccgca ttatcaggag cttttacttc ttctaaatta tattttccaa cttctaaatg
    14701 attaacgatc aactggcctt tttcaataga tgcctcggca aagtcattgt tgccgatgtc
    14761 ataagaatga ccagtaatga aatgtttggc tttggtttga tcagttgtcc aagtgtacaa
    14821 tccatctgtg acactttgga tgtatttttt gacgcttggt gttccttctt ctttagaaat
    14881 aataaattct gctccattta gtgcttcgtt ttcggcagta ccgatttttg taactttcaa
    14941 cgttccatca ttaccgactg tatttttagg gtagagatgg atagtatcta gttcttctgt
    15001 cccgtattta taagagccat ctgcttgttt gatcatctca tatacaggga aagctaaaac
    15061 catgtttgcg gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt agactgcatc
    15121 tttcccattt tgttttttag gtaaagataa agtgacattt ccatcagcat ctgtcgttcc
    15181 tgaagcaact ggtgtaccag gagtcaaaga ctggactgct tgttttgctg catccacgga
    15241 cgctccttta tctcgttgcg cataaaattc ttgagtgacg ttataaactg aaaatgaaat
    15301 atcggctaat ccttggtatt gatcgaattc gctcatttct ttcccgctgt tttggattaa
    15361 aggatcgggt aaatcagtca ttttcttttt atgaaggatg acttgagctg ctgtctcctc
    15421 tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac
    15481 tcctaacata acgtttattt ttttatgatt tttcattttt tcactcctgt tctttttttg
    15541 ataaacaacc agattgcaaa gcttgcgaat accataccaa caacgcctaa aagtgtattt
    15601 ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt cttttttatt gactactttc
    15661 aatatactgt ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa cggaatttct
    15721 gtttcactta aaatataacc agaaggtgct tcgatctctt ttaatcgata acttcctgtt
    15781 ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag cgcatctgat
    15841 tcgttctttg tccacttgta tccgttggct gtttgctgga ggtaatttcc tgctttgttt
    15901 ttaataagga atactgcgcc agctaatcgt ttatctggtt tatttgtctc gattttgaca
    15961 aattgcttac tccctgtata tacagagact ttctttgtac tgacaccaaa accaaaatcc
    16021 agtgttccgt tgttgttgat ttccttgttc gcctgtgctg tactgcttag ctgcatctga
    16081 taagaaatgg tcagtttttt tccagcatat ttttccaacg aagggatcga aaataaaaca
    16141 tcaaatccgt gaggtttctt gtgtatgaca aagccttctg tcagcttttc tccattcgat
    16201 gaaatcgtta aactattagg taaaaacgtt aatgcttcat ccgcactatc tgacaattcg
    16261 aacttttgat agtcaaggat atttacagga attgtcgtat gcaaggaata agtgattttt
    16321 tcaccaatcg taaaatcatt tctaggctct tctaatacct tctcaaaagg tggatcaggg
    16381 tagtcgtttt cttcattttt aggatacaaa tggatagttg aaagtttttg attgttttgt
    16441 ccatgtacag gaagaacaac taccatattc tctgcctttt ctttgacgac ttctggtact
    16501 ttggattcaa tgaaaagata cgctttatct cttttttctt gatctttagc ggccagagaa
    16561 aaagaagcga taccatcttc gttgttaaga gttgtagttg ttgcttctgc ggtaaacata
    16621 ccggaagacg caccgttttt tgcgatctct gcttgtgctt cttctaccgt tttgcccttt
    16681 tcccgtagat ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac accatttaat
    16741 cctcgatacg tttgtaataa agctttttct tgtccgtcat ttggatgatt tttcggcagt
    16801 tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct
    16861 gttgctacct gagtaaaagc tggtttaaat agtaacaaga agagacacat actaagccaa
    16921 ccaagttttt tcatatttag ctgccccctc tttctttttt caaaaaaaga taccaaatag
    16981 aaaacgcaca accaatcatc cctactagat agattcctaa acggccaatt cctcccgttt
    17041 caggtaatgg tacttttgcc tgattcgtga tgtctaaaga aatctggttg tttttggctc
    17101 ctggtgacag aacagattca tgatcttgtc catccacttg aattgaccca tcttcgtgta
    17161 taactatagt aactggctct tttagacctt ggtatccttc tggtgtaaaa gtttcggtca
    17221 gcgtataagt tccaggtttt aaattttcga atagaaaggt atctgtttct tgtccatctt
    17281 ttggcgattc taagtccatt tctggtccct gcagtctgaa ttttgctcct tttaatgtct
    17341 gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag
    17401 tgagcgtcca tacattattt gtcatctcgg tattttttcc cgtgtaaatg atttttccat
    17461 atttatcgat ttggaattca taatttcccg gcgaagtatc atatccggca ggaccagctg
    17521 tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa actggcaaga cctttttcat
    17581 tcgttgtttg gctgtcaatt ggctgataag taccatttgc ttcttttttc tgtagggaaa
    17641 atgctgctcc tttaagattt atctctttgt ccgtcccttg catcgcatac ttacgtactg
    17701 ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca tcggaagtag
    17761 tgactgtttt tccatcaatg gttaccgtac catcagaagc gatcttgatt tcccaagtag
    17821 tctttttgct taatccatgc ccttctggag cttttgtttc tgtcagcgta taggtcattt
    17881 ctttttgcaa tttgacattt tctggaagag aataggttcc gtcgccatga tctgttagta
    17941 aagtatcaat agaatctcct gttaatttga aaacagcgcc aataagatcc ttttcacctg
    18001 tagaggaatt tttcgttatt ttcaagttta acggtgtgaa ttgtttagta tttttccatg
    18061 tcattgcatc tatttgttga gaatcgtatc ctggtacagg taattctttg attgtttggt
    18121 aactgaatgc ttgaccttga ttattgtatt gaggtagtga taatatctct tgataacttt
    18181 ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt gttgtatctt
    18241 tagctggttt aatgattcga atatatccgt ttttccaagc tgcagcattt gtcgtatgtt
    18301 cccgttgaat ctcaaaagta acttgatctg gacgatcagc tagattgttg tcaaattctt
    18361 cccataactt tttgatgtga agactgactc cgggagcttt agcagatggt ataccgaatt
    18421 cagctaattc attggtatca atgtttggct ggaaagttgt cctgccgttc atttgatacc
    18481 agaaatttgg atggaagtcc tcattttctg tttggattct cacttggtaa tggatttgga
    18541 tttcttggtc ttttcctaaa tagatctgat tgctcttgat ggtatttcct tctatggaaa
    18601 tagatggagt gaccgttgta ggacttgtcc ccacactctt gactgaaagt gtaccaggct
    18661 gataaatgaa tggttctgcg attggatcat ttatttgtcc attgcttaca gttgctgaga
    18721 tctgtacagc ttttttggct aggtattcag agatatctgt tgcatgatca gctgattcat
    18781 agtaaagatc gcctttttca tctgatgaaa ccatttgtcg catacgagac tctacttctg
    18841 cttttgagag accagctgcc ggatcgcttt gaagttggat gccaagacca tgtatttcga
    18901 ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc gataaacgta ctgtcgattc
    18961 ttctggatag attgttttgg tcaggtgcat catagattct tgagattaga gaagtatttc
    19021 ccggccgatc ttgcgtatta gaaaactgag ttccgtaata attgctgctt gattgtgcgt
    19081 gtacccgctg tactttatag gaaaatgttg gtacaccatc cgtcagcaaa acgatcactt
    19141 ttttatgtcc atttggaacg gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa
    19201 aagtaccacc atttgtccgt gaaggtgtaa tggattttac ttcatttttc actgaatcaa
    19261 atgaacccat ctgtactgca ccgttactgt agctatatcc ttcgcttgag tagccgacat
    19321 atcccatatt gattttgtct gtgataccgc tatctgctaa agtatcgaca aaacgatcga
    19381 caccaatctt tacttcaccg atccgattat tgtcgttcat acttcctgac cagtctacga
    19441 ccaatacgag atcaagaggc gtgatatctt tttgtacatt tccacgagca ttcaaataaa
    19501 catcaaacaa tccgggtgta cttgtttcct ttgcatactt tcgtatcgca tagtctgctt
    19561 gattaccggt tcctccatat tcgatatacg aatgggtccg atcattagga tctccattcc
    19621 aagaatttat accatcccat tgatttgttc cgtttttatt gccttgatga tcaagaacat
    19681 ttgtatttcc tgtaggttgc caactggctt taggataagt gccctgatca tctgttgtat
    19741 attctgtagg aatcgtttga ttagatggca cactcacagc tggcaaggat aatgtaggca
    19801 atgtaaatgg gctgacagaa ttttcgtttt cttttgatga ttcttcttct atttcgtctg
    19861 gctttggtac tgacacatct gtatctttct tattttctgt ctcatcaata atgtcatatt
    19921 ctttattatc gacgatcagc ttgtttgcct ggctgtggta aagtgctgga tcgattggaa
    19981 aactcagatc gatagtttgt ttagtgtttg atgagatttc caacgtaatt ttcccgctat
    20041 ttacctgata agcatagcca tctccttctt ttctttccac atcacttaag taattttcga
    20101 ctagaccaat ttctttttct atcttttcag cactggtgtt attcgcattt agcttgatgt
    20161 tcagtgcttt ttttccagac tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg
    20221 ttggatcatc tgtagtgtct gctattgcag ataatggcac aaagtttgat aaactcaata
    20281 ttatcagcat gaaaataact ttcagtttct tccctgtggt cgtcattttt cccctccttt
    20341 ttcttttttt ctgttttgac gtgaatagtg aaaattatta aaataaacag gcgttacata
    20401 attttattta ttttaatatg ataattatta tacgaagggc gctaaaggga atcacaccaa
    20461 ttttcatacc aattggtttc atttatgaag ttgattcctt cgattaatga gcgtttcatt
    20521 gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt gttggaacga aactatagaa
    20581 agatggaaaa aatcattcga attctgtcca tttctgacaa gtggatgact aaaaaagaac
    20641 ttgccaaacg aattggtagt tcagaatcta catttgtaag gtatatcgaa gagatcaaaa
    20701 tccgctgggg aacggatgta acgattcaat cttcacaaaa attggggtat cgtcttgaac
    20761 atttgaatgt accagcttat ttaaatgttc tgatggaaat gtcgcaaagt tctacaaacg
    20821 gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac gattgagtat tattgtgaaa
    20881 aaatttccat cagccggtct tcttttacac gtaagttgaa acaatgcaat caaattttag
    20941 aaagctattt attaaaagtg atcgttgacc aaggttttca gttaactagc cttaaaagtg
    21001 agctccagtt gcgtatcttt gttacctttt tctttttaag ttactacgga agacgcacgt
    21061 tgccatttcc aatcgaaaaa aagaagatca aagaaattat ggtcaagaat aattgtcaga
    21121 tgaatttgtt tcaggaagaa agcccatatg agcaatcttt ctttattatg tattttgttg
    21181 tgcatctcat gagagaatcc caaggaactc catttgaatg gaaagctgga gactcttatg
    21241 tacagttgat aaatatcgat aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat
    21301 tgaagtacag cagctatata aaagtagtga actattttgt caatacgatt ttttcagaat
    21361 tttcggagaa agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg tcctactgtt
    21421 cgttttcatc cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc
    21481 aaatttattt tttctcccgt attttcccat acgatacgac tcatttgacg atccgtgcaa
    21541 cttactttgc acagcagatg caacaccgcc agatacgtct atatgctgga tttcgtgcag
    21601 aacttcaatt ttattccact ctactgaatc agaatctgat ttattattta tctagctata
    21661 tcttttggat gcttatcggt aatcctgaaa ttcaccatta tacttctgat aaaaaaatac
    21721 tgattatcag cgacttaagt cttcgacata gtcaatatat tgaagaatat atcagtgata
    21781 ttcttgctgt gcataaaatt cactctgaga caactgctat tactgaagat cagctgtcga
    21841 aatacaatct tctcgagtat gatctgatcg ttacgaatca acccattttg aatgcccatg
    21901 ttcctcatat attgattgat gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct
    21961 tattcgagtt atgaataatc aaaaaacagc tatgtataaa ccaccctgat aaaaatttca
    22021 ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca acgagattta
    22081 tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag atccgtcaaa
    22141 gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc cgacgattga
    22201 atggagaaat gattgattct taataattcc agccattttt cgttcattgg agaatcagcc
    22261 gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa gtataacctt
    22321 tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt ttcgatcccc
    22381 aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg gactatgtga
    22441 gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata tttataaaaa
    22501 gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg gctgatgtgc
    22561 tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc gttcgataaa
    22621 ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat acgtggatag
    22681 cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca agtacccatc
    22741 ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag taaccataat
    22801 agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt catatgcatg
    22861 atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct cattatataa
    22921 agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag gataactatt
    22981 tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg aatcttcttt
    23041 ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa
    23101 attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc gttttttttc
    23161 tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg gtgtttgcaa
    23221 tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa tgatgctgac
    23281 gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc
    23341 agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt ttattaacct
    23401 cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa tccgcctagc
    23461 attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa aacagcaaaa
    23521 ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc aagcagaacg
    23581 cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa gaaatttttg
    23641 atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat cacgtacatg
    23701 aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc atttaaggtc
    23761 atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt taataactgt
    23821 agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac gatcccattg
    23881 atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc agtccacgta
    23941 tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa gataccgaca
    24001 atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt atagcctgaa
    24061 tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg ccctttcgtg
    24121 agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt
    24181 tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc ttcaatgtat
    24241 ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa
    24301 acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga aagtccattc
    24361 gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc gacgatcaag
    24421 ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg tttttcctcc
    24481 ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc taaagtgaca
    24541 atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc tttcgataaa
    24601 accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc atgataaact
    24661 aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag aagacgataa
    24721 agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa cacaagttta
    24781 tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg caatcttgga
    24841 tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga aagaaaaaat
    24901 acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga ttgaaggatt
    24961 tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac tgatcatgcg
    25021 cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc tgaagtataa
    25081 ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag ttttgcccat
    25141 acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa tcattttgac
    25201 aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga ttacagttat
    25261 tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg ataaagaaat
    25321 caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg aatggagata
    25381 ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt gcttttatct
    25441 tttttctttt gggattcatt actttacaga tattaaacca atctgcatac agagaaacag
    25501 atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag atacaaagat
    25561 atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga acaaacatgg
    25621 aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta
    25681 aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat
    25741 taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat gaagacagtg
    25801 gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat caagatggcg
    25861 ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta gcgacttttc
    25921 gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt atcagttatt
    25981 acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa aaagaatttg
    26041 ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat agtttggaac
    26101 atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc gctcaagcat
    26161 tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc gctcggaatg
    26221 attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat atcgaacaac
    26281 ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt ctccataacg
    26341 atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg gttattttgc
    26401 tggataatgc attaaaatat acgaatccag aagataagat cattttggat tcatctgtta
    26461 accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt gaagaagatc
    26521 aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa gagactggcg
    26581 gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat ggacagattt
    26641 ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg ttgaagccta
    26701 aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt
    26761 gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttctttaa
    26821 gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat tgcggtcttc
    26881 aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat cttctgtcac
    26941 tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa taactgtatg
    27001 tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat cttccaattt
    27061 ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga cgatttcttc
    27121 acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac
    27181 ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta gttgaattgt
    27241 atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc
    27301 agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg gatccgttgt
    27361 attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc cagtttctaa
    27421 tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg tatcttctgt
    27481 tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag aaatatctcc
    27541 tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat aggtatcaaa
    27601 agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat ccatccgatt
    27661 ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag atgctgcttc
    27721 atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca accgttcaat
    27781 tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat ttttctcttc
    27841 catgtaaatt cctcctagca aatagattta tgattggtat attgttactt ataaatttat
    27901 tctataggag acgattgttt tctacaaata tttagattgg aatttgatta aaacaattta
    27961 attgaaattt gaaaacattt atgttaatga aagtcatatt ctaataaaat gtcatttgtt
    28021 tctatatgaa tttttatgaa aaatttcata taatttccac tcgttgtgat ggtttaattt
    28081 atccaatcca tcaataagca aacaacttat ataaaaaagg attaattctc aatataattg
    28141 ctcctaccat agaataaatc ttcatgtaaa gtaatgaagt accaactatt tctaatactt
    28201 ctttaagaat tatcccaaca atatcgttag acaaaccatt attgaataaa tggagagcca
    28261 atcgattctg aaaaaatgtt ttctaatttt tcggaaataa aagaaagagc ctcctgccat
    28321 tagaacgaac caaatccatt tcattgctca tgaagtaacc acatagctaa aaagaggata
    28381 aatcaattaa aagatcaaaa gaatgactgc actaataaaa taactaaaaa tgtatcttct
    28441 tacttcatat cttttagcta gtacttagtt aaaaatttgc ttcctaagat caagacaaac
    28501 tgtctatctt catttgatga aatgcggtaa aatatagaaa atgaacacta ttggagggaa
    28561 tttgtaatgg acagcaaaac tagaaaacaa ctgcaagaaa agttaaagca acgtacacac
    28621 ttgctccctt ccgtctttca acagttttta gaagaaaatc gttcttccct ttcattaggc
    28681 agtcgctatg aatacgcaaa agattttcat ctgtttactg attatttgag aaaagtattt
    28741 tctgaagaag tggatgatca gctgattatc catttagaaa aacaagatta tcagaatttc
    28801 ttagctaaaa tctatcgaca tactcgttcg ttccaaacta cagcgaacca agaaacggag
    28861 caattgtttg aaaatagcta ttacgggatt tcaaggaagc aatattcatt aaatcatttt
    28921 ttacgatttt tgtatcaaaa aggactgatt gaagaagaga tttctctttt gggtacaagt
    28981 ttaccggaaa caaccaaagc agctccgcga tatatcgatg cagaactttt caaagatttt
    29041 gagcatttgt ttatcccgat tgaaggcttt caaacaagca gagaagctag ctttgaagaa
    29101 aagaatagac cgaggaacct tgcaattact gccattcttc tgtattgcgg attaaagatc
    29161 catgaagtag tagaacttaa acgtaaagat gtatctttga ccaagcagat tcttaatctg
    29221 aatagaaaag aaaacagact gaataaagta ccaataccaa aagaagcgat gccatttctg
    29281 gagaactatt ttaagttaac agcacaaaat acggacgaaa attcttttgt ttttcgttcc
    29341 tcacatgatc agcaaatttc tcctcggact attcgccaaa ttttgagcaa aattacagta
    29401 tataaaaacg tatcgccaga actatgcaga aaaacatatc gttattatgc agaactatat
    29461 ttagatgatg ctgacgcggt aaattattta tgtggtaatc gatacctctc atcccattct
    29521 tttcaagata tctgggaaaa gatggttgat ttttcttatc acgaattagc ggcattcgtt
    29581 cgggtttctt ccatataaaa caaatgtatg atcttttttt cacaataaac tatgttatta
    29641 tataaaggat tatgttacga ttatcttgaa tttgtatatt acttaacaaa agataatgga
    29701 gtgtgaatga tggagaccat aacgtattcc cctttgaact tatttacgaa ttttcaagaa
    29761 gctgcagcta acttttccga gaccccgatt atctttgatg agccactttc agctttccca
    29821 gaattaggac ttgaaacaac ttacaaaaaa tgctgcgaaa gtgtgttgaa acgggcttat
    29881 caattggctc atcttggagt gaaatccgga gacaaagtta ttatttataa gagttccgct
    29941 tttgacacat accttctagc tgtttctgtg tcttaccttg gtgcagtgcc tgtcatgact
    30001 tcttatcatc taccaacaac aacaatggaa gtatttatcg atcgtttaga agaccctttc
    30061 attttatttg atgatgagac aaaaaagcga gtgagagaaa tttcaaatgg aacaaaatca
    30121 aaacagattc c
//
LOCUS       DACDNO010000032        29901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDNO010000032 DACDNO010000000
VERSION     DACDNO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.032"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1097
                     /locus_tag="IWI22_001499"
     CDS             <1..1097
                     /locus_tag="IWI22_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP9168895.1"
                     /translation="AGYSADSAANLLRQDPVFKAVLDRKELASQSSLSRFLDRLSEEN
                     IHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQTNYNTHYQTYGYHPLVA
                     FDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPNTDILVRGDSGFATPGVY
                     DSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREVQYFSTTYQAQSWSQSRR
                     VCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGTMENFIKEAKAGFYFDKT
                     DSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHSIRLKFLKVAGKLVQTGR
                     RVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1538..3178
                     /locus_tag="IWI22_001500"
     CDS             1538..3178
                     /locus_tag="IWI22_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9168896.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3332..4654
                     /locus_tag="IWI22_001501"
     CDS             3332..4654
                     /locus_tag="IWI22_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9168897.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4716..5147)
                     /locus_tag="IWI22_001502"
     CDS             complement(4716..5147)
                     /locus_tag="IWI22_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9168898.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5211..6047)
                     /locus_tag="IWI22_001503"
     CDS             complement(5211..6047)
                     /locus_tag="IWI22_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9168899.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(6237..6539)
                     /locus_tag="IWI22_001504"
     CDS             complement(6237..6539)
                     /locus_tag="IWI22_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9168900.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(6565..6996)
                     /gene="ruvX"
                     /locus_tag="IWI22_001505"
     CDS             complement(6565..6996)
                     /gene="ruvX"
                     /locus_tag="IWI22_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9168901.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6993..7259)
                     /locus_tag="IWI22_001506"
     CDS             complement(6993..7259)
                     /locus_tag="IWI22_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9168902.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7513..8238)
                     /locus_tag="IWI22_001507"
     CDS             complement(7513..8238)
                     /locus_tag="IWI22_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9168903.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8604..8674)
                     /locus_tag="IWI22_001508"
     tRNA            complement(8604..8674)
                     /locus_tag="IWI22_001508"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8640..8642),aa:Gly,seq:ccc)
     gene            8791..9543
                     /locus_tag="IWI22_001509"
     CDS             8791..9543
                     /locus_tag="IWI22_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9168904.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9559..10038
                     /locus_tag="IWI22_001510"
     CDS             9559..10038
                     /locus_tag="IWI22_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9168905.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10289..11869)
                     /locus_tag="IWI22_001511"
     CDS             complement(10289..11869)
                     /locus_tag="IWI22_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9168906.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11862..12605)
                     /locus_tag="IWI22_001512"
     CDS             complement(11862..12605)
                     /locus_tag="IWI22_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9168907.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KRLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12781..13557)
                     /locus_tag="IWI22_001513"
     CDS             complement(12781..13557)
                     /locus_tag="IWI22_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9168908.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(13805..14641)
                     /locus_tag="IWI22_001514"
     CDS             complement(13805..14641)
                     /locus_tag="IWI22_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9168909.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(14652..15581)
                     /locus_tag="IWI22_001515"
     CDS             complement(14652..15581)
                     /locus_tag="IWI22_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9168910.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15773..16126
                     /locus_tag="IWI22_001516"
     CDS             15773..16126
                     /locus_tag="IWI22_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9168911.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(16200..16856)
                     /locus_tag="IWI22_001517"
     CDS             complement(16200..16856)
                     /locus_tag="IWI22_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9168912.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            17082..19016
                     /locus_tag="IWI22_001518"
     CDS             17082..19016
                     /locus_tag="IWI22_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9168913.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(19102..20325)
                     /locus_tag="IWI22_001519"
     CDS             complement(19102..20325)
                     /locus_tag="IWI22_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9168914.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(20319..22217)
                     /locus_tag="IWI22_001520"
     CDS             complement(20319..22217)
                     /locus_tag="IWI22_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9168915.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(22211..23329)
                     /locus_tag="IWI22_001521"
     CDS             complement(22211..23329)
                     /locus_tag="IWI22_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9168916.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(23412..24245)
                     /locus_tag="IWI22_001522"
     CDS             complement(23412..24245)
                     /locus_tag="IWI22_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9168917.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(24238..25155)
                     /locus_tag="IWI22_001523"
     CDS             complement(24238..25155)
                     /locus_tag="IWI22_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9168918.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(25382..26506)
                     /locus_tag="IWI22_001524"
     CDS             complement(25382..26506)
                     /locus_tag="IWI22_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9168919.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            26689..27597
                     /locus_tag="IWI22_001525"
     CDS             26689..27597
                     /locus_tag="IWI22_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9168920.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            27738..29351
                     /locus_tag="IWI22_001526"
     CDS             27738..29351
                     /locus_tag="IWI22_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9168921.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9303 a         5874 c         5116 g         9608 t
ORIGIN      
        1 ttgctggtta ctcagctgat tctgcagcta atctcctgag acaagatcct gtgtttaaag
       61 ctgttttaga taggaaagaa ctcgcttctc aatcttcact ttcccgattt ttagatcgac
      121 tatctgagga gaatatccat gaacttcaag ctttgaacca agaacttatc gataaagcac
      181 gcctcatccg taatgatacg gaattaatca ttgatttaga ttcgacccat tcagatacat
      241 ttggtcacca agaacaaacg aattataata cacactacca aacctatggt taccatccat
      301 tggtagcttt tgacggattg actggtgact tcttaaaagc tgaactacgt tcaggaaatc
      361 aatatacatc aaaaggcgta aaggagtttc tcacaccttt attagagcac tataatcact
      421 ctctaccaaa cactgacatc ttggttcgtg gagacagcgg gttcgctaca cctggtgtgt
      481 atgattcgtg tgaatcaaaa aagagtcatt atgttattcg actgaagaat aatcgtagac
      541 taggtcaaat agctgagaaa tcagtacttt atggcgataa tcaaaagtgg gaagaacgag
      601 aagttcagta cttctcgacc acttatcaag cacaatcctg gtcacaaagt cgtcgcgtgt
      661 gtatacgctc aacacgtgaa gcgggcgaac tactctttcg acatgaattt atcgtgacga
      721 atctatcaga aaatgtttct cctgatacca tcttttctat ctatgccaaa cgtggcacaa
      781 tggagaactt cattaaagaa gcgaaagctg gcttttactt tgacaaaaca gacagtcctc
      841 gctttttgga gaatcatgtc cgaatgatgc tcagcttgat agcttacaac ttagtcaact
      901 tcttaagaac gattggcttt gaagaagtcc aaaagggaat gaccattcat tctattcgat
      961 tgaagtttct gaaagttgct gggaaattag tccaaacggg tagacgagtc tatctcaaat
     1021 tatctagtta tcatgtgtat cagaatgaat tctacagggt ctttgctcgc ctgaggcgag
     1081 ccagtcaatg gatctaatcc aacaatctaa cgattttttt actgggaagt tatccaggga
     1141 gaagtatgct caaaatgcct tacacccaaa atttattcct caatattttt gaacagtgaa
     1201 tgtttgaagt aactttttag tcaaaatcag ttgataggga tattagtgat acatatttca
     1261 ataaaatgtg ccaaagaatt aaagctatga attattcagg attctattta tctatctaac
     1321 atatccttta ttcttctact tttctttctt tagcatataa gagatacgca tagtaatcaa
     1381 gcaaggattg ttgatataaa aagaacaaat accgctattt tgatttcttt tgactctatt
     1441 actcatataa ataaaattta ttatacctgc tctttttgac tatatcctca tctgactcct
     1501 atacttcgtt tgtattaaaa cttaggagga atcattcatg cttacagggc taaacttatt
     1561 aaataatcct tttctaaata aaggaacagc ttttacaaaa gaagaacggg caaaatacgg
     1621 aatcactggt atgcttccaa gtactgtcca aacattggaa caacaatctg tccaagctta
     1681 cggtcaatac ttaagcaaac agaccgattt ggaaaaacgt atcttcttaa tgaatctatt
     1741 caatacgaac cggacattgt tttataaatt gatgggcgaa catttagttg aattcatgcc
     1801 tgtcgtttat gatcctgtcg ttgcggattc aatcgaacaa tacaacgaaa ttttcttgaa
     1861 accacaagat gctgtatttc tttcaatcga cgaaccagaa aatatcaaag caagtttgaa
     1921 aaacgcagca gatggacgcg acattcgttt gatcgttgtg acagatgcag aaggaatctt
     1981 aggtatgggt gactggggtg tgaacggtgt tgacatcgct atcggtaaat tgatggtcta
     2041 cactgccgcg gctggtatca atcctgcaca agtcttgcct gtatcaatcg acgctggtac
     2101 taataacgaa gaattattga ataatccatt atacttaggg aatcgtcatg ctcgtgtaga
     2161 aggcgaaact tactacgaat tcattgatca attcgttcaa tcagctactg aattattccc
     2221 agaattgtta ttacattggg aagattttgg acgaggaaat gctgcaacga tcttagaaaa
     2281 atacgaagat aaaatcacta ctttcaatga tgatatccaa ggcacaggaa tcgttgtact
     2341 agctggtgta ttaggtggat tgaatatatc aggtgagcaa ttgaaagatc aaacgatctt
     2401 gacatttggt gctggtactg ctggtgtagg tatcgcaaac atccttttag atgaaatgat
     2461 ccgtcaaggt gtacctgaag aagaagcacg ccaacatttc tatcaagtag ataaacaggg
     2521 tttattgttt gaagatacaa aaggattgac tcctggtcaa atcccattcg ctcgtaaacg
     2581 ctcagaattc actaatgctg atgaattgac aaacttagaa gcagttgtaa aagcaatcca
     2641 tccaacgatc atgatcggta cttctaccca acctggagca ttcactgaag aaatcgtgaa
     2701 agaaatggcg gctcataccc cacgtccaat catcatgcct ttgtctaatc caacaaaatt
     2761 ggcagaagca aaagcaaaag atttgattga atggacggac ggaaaagctt tagttggaac
     2821 aggtatccct gctgctgatg tggaatataa cggtgtaact taccaaatcg gtcaagcaaa
     2881 taatgcgttg atgtacccag gtttagggct tggtttgatt gcttcaacag ctacacgagt
     2941 aaatgctgaa atcatttctc aagctagtcg agcattaggc ggaatcgttg atgtaacaaa
     3001 accaggagca gctatcttgc ctccagtagc aaaaatcact gaattctctc aaacgattgc
     3061 tgaaactgtc gcaaaatcag ttgttgcaca aaaattgaat cgtgaagaaa tcacggatat
     3121 caaagaagct gttgaatctg ctaaatgggt tcctgaatat aaatctttag aagattaaga
     3181 ttgttatagt aaaagaacat acagaaactt tgtatgacgt ctaaaaagaa aaaagcgact
     3241 gcggcaaaac agcagccagc caactactgc tcttttgccg cttctctttg gcttttttca
     3301 tacgatacag aataagaaag gatctgtaaa aatggcacaa aaaacaacaa ccgcaactgc
     3361 agttgaggaa aagaaaaaag aagggttttg gtcaacaaaa attaccggcg taagtttgcc
     3421 cctttatttg atcatggtcc ttgtcctgat cattgcaatg gctttaggaa aattaccgac
     3481 gaatatgatt ggcccaatcg ctgttttggt cattttcggt aatttatttc attttatcgg
     3541 aacaaaaatc cctatagtca aaagttattt aggtggtggc tccgtttttg cgatctttgc
     3601 ctctgccgcg attgctacat ttgggatctt acctgagtat gtagtaaaat ccacagaaaa
     3661 ctttgtcagc aatatgggct ttttagactt ttatattgct gccttgatta ctggtagcat
     3721 ccttggtatg aaccgtaatc tattgatgaa agcttctgtt cgttttattc ccgttgcact
     3781 tatttcaatg gtcgtctcct tttttgccgt aggactcgta ggtatgctta tcggaaatgg
     3841 ttttgcaaat tccgttttat atatctcatt gcccgcaatg gctggtggtg ttggagccgg
     3901 agctgtacct ttatcaacga tttatgcaaa tgttttaggt acggacgctt cttctatcat
     3961 ttcccgcctg attcctgcga cagcaatgac aaatgtccta gctatcattg gtgcagcgtt
     4021 agttgctcgt gcgggacaat caatgccaaa attcaacggt aatggtaaat taatggaaaa
     4081 aggcgacctt ggcgatggaa aacctagaaa tgtaaaacct tccattccac aattaggtgt
     4141 cggtttgatg gtatcattga ctttcttcat cttaggacaa atctgtaact attttgttcc
     4201 taaagtccat gcttacgcat tcatgatcat tatcgttgtt atctgtaaaa tcgcaaacct
     4261 attaccagaa tattacgaag atgcagctat catgttcaac aacttgattg tcaaaaactt
     4321 gacagcagct gttcttgccg gtatcggtat tgcgttattg aatctaaatg tattggcttc
     4381 tgctttgaca tggcaatttg ttgtcttatg tttgacaagt gttatcgcga tttcagtagt
     4441 ttcaggattt gtcggtcgtt tgtttggttt atatccaatc gaatcaacta ttactgctgg
     4501 cttatgcaac aactcgatgg gtggtacagg aaatgtagct gtcttatctg cagctaatcg
     4561 gatggaactt attgcttttg ctcaaatggg caatcggctt ggtggtgcca tcgttttgat
     4621 catctcaggt ttcttgatgc aattactttc ttagtcaaaa aaaatacctg atcatcaagt
     4681 atcggcttga tgaccaggta ttttttgata aaaatttaca ggattttcaa aaatcttttt
     4741 tgcattttat cggaatgata accgatgcgt tcataaaatc ggtgtgcttc tactctgtat
     4801 tctgcagaat taagtcttat tgctgatatt cctcgtcttt tcgcttcttc tacgccagtc
     4861 atcaaggcct ttccgattcc tcttttttca gcatgtttag acacagcaag tgccataata
     4921 ttgaacatcg gatcgctgaa aatcgattca taggactccg cttgtacata gccagctgct
     4981 tttttagaag taggatcttc atagaccaag aaaaaatttt tttctggctc cttcaacagt
     5041 ttcttcattt gtctcaccgt aacggttaat ggtacatcat atcctaattg ttccgcattg
     5101 atttctcgta gtgcttctgc atctcctaaa cgcatttccc gcaacatcca aatcaccagc
     5161 caatttttat ttttttaaat gaattttttt atcacagtat actccttttt tcacttaaaa
     5221 aaagactttt cagtgatttt gacttgcttg aatccagatc cttctaatgt ttgcaagatg
     5281 acgaaagcat tgtaaagatt gatcaaaaga accacgatga caatcgcttg aaaaataaag
     5341 gtcacttttt ttggagacaa aaccctgcta aatcttgctc cccacaaacc tccaataaca
     5401 gcagcgggga taacataaaa caacatagat aaatcaaagc gcataaagct acttgttaaa
     5461 gcaatcgtta ccaattttgc cagttgagaa aagaaaatcg tagatagaga atatagtgtt
     5521 gcttctttta ttggcaatgc aaacatcagc atcaacaaag aaacattgac tggtccacca
     5581 ccaataccta aaaaactagc taaaaagccc agaacccccc cacatataag gtaccaagtc
     5641 catgacgtca aatgaaattt tggctgatga tgcttggtgt agaagaacgc aaaaaccaag
     5701 gtcaataccg tcaaaaaaat ttggatcagc tgcacatgct tctcgttttc aaataactgt
     5761 aaaaaaactt cgaatacaat gttgccgact actcctccaa caacagctcc actagatacc
     5821 caaccgacaa tctgccaatt cagtgatttc ccgcttgata cttgtcgcac agtcgaaaca
     5881 atggacatcg tgaataccgc aactgttgag taaaaagaaa tccctgcaac agaatgggcg
     5941 ccgataagat caagaatcgg tttgatcaac acgcctccac ccatcccgga aatagcgcca
     6001 actgtgttcg ccagaacgat caccacaaaa taaatcagac caatcatctt gtatttcctt
     6061 tctcttgttt atatctttta gcgattgtta ctcttttctt ttattataca gtaaaagaaa
     6121 caaacgaggc ttcgaaagct tttctacttt ttaaggaaat ttagtgctaa acgaaaaaac
     6181 cgggctctac ttttcagaga gtccagcttt tcttacacct tatcgattct tttgttctat
     6241 tcttcttccg ccatgaatgt attgaatact tcttcgatca tatcccattc tgcgtctgtt
     6301 tcgatttgtt ctaactcgcc ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt
     6361 aattctacat cttcatcttc tgaaacacca gctggatata aaagaacata gttacgtcca
     6421 aattcttctt gtccgtcaac tgttagcaag atttcgtata atgtttcatt tccttggtca
     6481 tccaccaatg tgatgtgttc gtgtccttcg tgttcgtggg aatggttatg ttctgtcata
     6541 agattacctc ttttcttttt attattaaaa taattgctta cctgagccgt ctaaataatt
     6601 ttgtaaaatc atcacagctg ctaatttatc gatgactttt tttcgtttcg ctcttgaagt
     6661 atttgcctgc tctactagca tacgttctgc ctgaacagtg gttagtcgtt catcttgata
     6721 atcaaccggc aaggagaata gttctttgat cttctctcca tatgacatgg aagcttctgc
     6781 ccttggtccg atggaattgt tcatattctt aggtaatcca actacaaaac ggtctacttc
     6841 gtactgcgcc actaattcct tcaaccgatc aaaaccaaac tcttcttctg cctcattgat
     6901 tcgaatgatt tctactcctt gagctgtcca gccaaaggga tcgctcacag caacgcctac
     6961 tgtcttagaa ccgacatcca agcccattac tctcataaat tcactccatg atttgagagg
     7021 tagtctttga caattacttc catgatttca tcgcgttcgt gacggcgaat caaatttcgt
     7081 gcatcacgat aacgaggaat gtaggccggg tctccagaaa gcaagtagcc gacaatctga
     7141 ttgattggat tgtagccttt ttcttccaaa gcccgataaa caatcgctaa tgtttcgctc
     7201 acttcttttt tgcgactatc gtcaaaacca aaacgtacgg tttcatctgt aaaacccatg
     7261 attctacacc tctttcccgc aaggtaattc cagcttacct tctacaccct tattttactc
     7321 gaagaaaggg aaaactacaa acaaatattc caaacttctg aataaattaa gaaacacgat
     7381 ttgtctgatt tttccttttt tctgtggctt tttcaaaagc cctctttcca taaagtatac
     7441 gctatatttc tttgaaagta tgtttttaca caattttttc ataaagttca taaaaaattc
     7501 gaattgctgc tactaatttt tcgttttcgt tgcttgccct gtctcataat tgatggtgta
     7561 gccatcttga acattgtaga taaagacatt gaattcaatt tttttgtctt caatgctttc
     7621 tgcttccaac tgcacacctc ttgctaccag ttcatttccc tcgaaatgag gagtgacacg
     7681 atagcgtaca tgatgatttg tttccttgat atatgccgca atatcatttt cgtgggcaag
     7741 catgtacgga gcattcaacg aacgtgtccc tgtcattaag tttttaatgt tattattttg
     7801 accagtcaac tggaaaccaa tcaaatggct gcggttgtat aaccattgtc cgtcacttaa
     7861 tttcttttgt ttccaacctg tcggatcaat atacaaaggt tctctctttt cagtaggaaa
     7921 caagtctttt cccaacatcg cattggcttg tcctactctg ttcaatttgt ctatatttga
     7981 atagctttcc catgtccctt tggaaagact aagatcctct tttgtaaact ccggctgatt
     8041 cccattgatt tctatttctt gatgttttcc atcatattct ggcacatccg ctacttgaga
     8101 agcttgcggt gtggaatcaa aaatggttgt aatttgttgg aagatatctg aatctttttg
     8161 tgtggtggaa ataccgagtg cccctgcaat cacagcagcg acgatcccca cgatcgcctt
     8221 ggttgttttt ttgctcattt ctttactcct ttactaacag ttccctaaat cttaacatat
     8281 gttcccctat aaataaatac tggaaagaaa tagaaacagt aaaaaaataa aaccagtttt
     8341 tagacggctt aagaatacaa aaaaagagac ggattcatcg tctctactca atcacaaaac
     8401 actatttaca aggtatttat tatcattcaa aaaatttttc ttaatactca aaactaaacg
     8461 gcttctgtac gacctcttgg taaacagtgt tcaaaagctt gcatctgcgg taataagtcc
     8521 aaccaagtca aaaacataag aagctttttt gcttgactat tcttattact tgatgcaggg
     8581 cagctttttc acagcctctt ttaagcgggt gacgggaatc gaacccgcga catcagcttg
     8641 ggaagctggg attttaccac taaactacac ccgcgataca aaaacagtat acgcctcttt
     8701 cactccttcc tcaagttttt ttattcttta cgcttccgat atgttttgta gtaaaataaa
     8761 actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga tctgttgctg
     8821 gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat gtatcgggta
     8881 gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt cagctatgtc
     8941 actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat ggaacaaatt
     9001 accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc acgtaaatat
     9061 gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat cgaccgagac
     9121 cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag tggaacaatt
     9181 atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat tggaggattg
     9241 ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt atcggaattt
     9301 ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg cttaggaagc
     9361 aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt tcaaattacg
     9421 aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg cggaacagaa
     9481 gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca gcttttttat
     9541 taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag ttttttagcg
     9601 actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg ttgtggtctt
     9661 tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg atctgctgct
     9721 tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt ctctaaacgt
     9781 ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt ccctggtggt
     9841 ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc ggctcattat
     9901 gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt atcagaagtc
     9961 tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat gaaacgaaaa
    10021 aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga gacgttcagc
    10081 cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg agcaataaga
    10141 agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt ttacataaaa
    10201 ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa aggggctgcg
    10261 acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac gttcgttgat
    10321 aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga atattcacta
    10381 accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata atactgatga
    10441 tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg ttaaataatt
    10501 ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa taacgtagtg
    10561 acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa aacaaaggca
    10621 gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc tgcaatatcc
    10681 atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa gaacggataa
    10741 acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc tgattaactg
    10801 ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta ttatctaaat
    10861 cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt ggagttaaca
    10921 aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga gcattgtaac
    10981 taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt ttgaactcac
    11041 tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt acagcaagga
    11101 aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt tgagaaagtg
    11161 gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga tctgttaact
    11221 cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat gcgatcagca
    11281 tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac actgctgttc
    11341 gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa aatagagaaa
    11401 acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc catccggtta
    11461 aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca actaccaaaa
    11521 tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact tgcaggtcat
    11581 ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct aacataccaa
    11641 aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata gcaaacatag
    11701 caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac aaaagataga
    11761 tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc tgtggattaa
    11821 catataaaag attaactcga attaattcta ataattgtcg cttatacata cggtccatct
    11881 cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc atcaggtgag
    11941 ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt caaaatagcc
    12001 agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat gactgttttt
    12061 ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg aggatcaagt
    12121 ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt gccgatcaaa
    12181 atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg atgactatac
    12241 atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt gtctaattca
    12301 taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa aatatctggg
    12361 gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt caatttcatt
    12421 ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaatct tttaatcgta
    12481 gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg tcttatcgag
    12541 agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt gatgaattca
    12601 atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg ttattggtgg
    12661 agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc tgctacttgg
    12721 gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc tttgttttct
    12781 ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat caatacttct
    12841 ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta ctctgctaat
    12901 agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata aatactcata
    12961 ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt catctttcaa
    13021 tactaggcct attttatttt tataacctgg catgctcatt agataaagga tttcatcgta
    13081 atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt tcgtagcatg
    13141 ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt ttttattttt
    13201 ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat taataacagt
    13261 tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat tcttttctct
    13321 aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt ctaggaaaaa
    13381 aatatctgag gaatagattt cattattttt aatgaattca acaaattgta taacgttcaa
    13441 tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt ccaaaatttc
    13501 tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg ttaccataaa
    13561 aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt caacatacat
    13621 gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa accgatatca
    13681 aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca tcatgcaaaa
    13741 aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta tttattttgt
    13801 tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc tcagcgtttg
    13861 taacaactac agatgggatt tcacgcttga atggttgata atagtctgat tggatggttc
    13921 cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata tgcgtcaaca
    13981 aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac aagaagatat
    14041 ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt tttttgccaa
    14101 tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt tcatctgtga
    14161 tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg tctaagtagt
    14221 ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc atctccggat
    14281 cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg atcgtagtgt
    14341 cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc agtacataag
    14401 cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg tgtgcctttt
    14461 cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa agagcagata
    14521 aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga tcgagtttgc
    14581 cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa aaccgcagca
    14641 tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat cagtccgatc
    14701 agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt tttgatatcg
    14761 accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag acttaacaaa
    14821 gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc caataacagc
    14881 atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct agttggtaaa
    14941 taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc tgtatcaaaa
    15001 aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg attttttggt
    15061 tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc tgctacttta
    15121 gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca gacaccaatc
    15181 acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca agaattgcca
    15241 aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt cagcataaaa
    15301 gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat cggtacgatg
    15361 ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat agagaagata
    15421 cgattttcag gaagccatct cttcacacgt tctggggtca aaaatacttg taaggcgcct
    15481 gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga taagaaaatc
    15541 gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct cctttttatt
    15601 tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaacaagt tgctctgtaa
    15661 atttttacac aactcttatt ttttaccaaa gcacttcaat tgactttttc tgttattccc
    15721 attaaattaa ggtctcatac atatcacttc tatcataaaa gggagaataa aaatgaaaca
    15781 aaccactaac ttgactgaag ttcaagacat actgcaacag tcatccgttg ccgtcattta
    15841 tctttcgatg cctaattgca gtgtttgtca cgctgttcgt ccacgactag aggaacttct
    15901 cacacattat gatattccgg cacttcattt ggatgcattt gaaacaccag aagtagctag
    15961 taaatttgaa gtgctgactg cgccagttgt cctgattttt catcaaggaa aagaagtctt
    16021 tcggcaagca cggttcatcg actttaaaaa aatccgttat ctactagatg aactgacagc
    16081 agaagatgat tccctttctt atgaagaaat cttccgaaca aaataatcgt tgtccaaaca
    16141 gaaaaaagcg attgaacaag tgaaagtttt gccttcattt gttcaatcgc tttttatttt
    16201 tattctgctg tattttcgga atgcgcattt gcgtcagaat gcaagaaata gatcaaggtt
    16261 tgcaattcat tcgtcaaatc aacattttgg atcaatacat ctgacggagc attcaatcga
    16321 gcagcggtaa agttcaagat tcctttgatt cctgcttccg ttaactgatt gactacttct
    16381 tgtgctcttt cagcgggtat cgttaaaatt gctacttcga tttgttggat cttgatttgt
    16441 tctgacatat cttccattgg ataaacgggg ataccgtcta cgatgcgccc aacgatttcc
    16501 tcattcacat caaaagcaca actgatacgt atgctattac tttgatggaa tttgtatttt
    16561 agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa ttgatcgtcg
    16621 tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat cataaccata cccacgtttt
    16681 cctaattcgc caaaataaga aaaatctcga cggatcgttg cactatctac ttgaacggct
    16741 tcacttaatt cagttgaaga cacttttttc tttccagcat tatttaaaat ccttaaatag
    16801 cgataataca aaggcagtct tttagctgtc gcctttggaa taatatgatc tttcacgaaa
    16861 tgacctccac gtattgtcta attacatctt tgatttctcc atcatagcag tatttatagg
    16921 aaaaaatcaa ttccattgct cagatattgt gaaattttat tctttattca tttatttatc
    16981 atttctgcaa atctcgcagt ataatggaat ttatggtaga ctactaattg aaaaatagtt
    17041 ttttatttga ggagaattca caaagtagaa aagaggattc catgatatta ttacaagcca
    17101 atcagattgc tcgccatttt ggtgcagaag tcttatttga aaacatacat ttagaaatca
    17161 gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg actttattaa
    17221 agatcattgc tggaattgaa gcacctgatg aaggaaagat cgctaaaaat aaaacagcga
    17281 ctctcggata cctcgctcag gacacaggac ttgattccaa tgaaacagtt tggaacgaga
    17341 tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag ctagagattt
    17401 tcattagcga aggtacgcca gacacacctg cttatagttc ttatttaaaa gaatacgacc
    17461 ggctgcaaca cgatttttca gatcgaaacg gctatggtta tgaaaatgaa attcgttccg
    17521 tattgcatgg gtttaaattc gattcttcat tttatgatca gaaaatcgat actttatcag
    17581 gcggacagcg cacacgttta gctttagctc gcatgctttt gcaacaccct gacattttga
    17641 ttctagacga accaacgaac catttagata tcgagacact ttcctggctt gagaattatc
    17701 tgcaaagcta tactggtgca ttattgattg tttcccatga ccgttatttt ttagataaag
    17761 tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa ggtaactatt
    17821 cgaaatatct agatttgaaa gcagaacagt tagcaagtga atggaaggct tatgaaaagc
    17881 agcaaacaga aatccataaa ttagaagact tcgttgctcg gaaccttgtt cgcgcctcta
    17941 ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg ctggatcgtc
    18001 ctcaagggga tgaaaaacaa gcaaactttc tattcagtat agaaaagcct tctggaaatg
    18061 tagtcttgca aatcgaagat gccgctatcg gatatgataa tgtcacctta tctgaaccaa
    18121 tcaatctaga tatccgtcgt caagaagcga ttgctctagt aggtccgaat ggtatcggga
    18181 aatcgacgtt gctgaaatca atcattcacc agatcccttt tatccgtgga aaagaaacgc
    18241 tagggacaaa tgtcaccatc ggctattacg atcaaggtca agctgatctg catacaaaca
    18301 agacgatcct agcagaattg tgggatgagc atcctaccac tccagaaaaa gagatccgaa
    18361 gtgctttagg tgctttctta ttttctggcg aagatgtaga aaaaccaatc cctctgctaa
    18421 gtggcggtga aaaagcacgg gttgctttag ccaagttagc gatgaacaaa gaaaattttt
    18481 tgattttgga tgaaccaaca aaccatttag atattgacaa caaagaagta ttagaaaatg
    18541 cattgatcga ttatgacggc acactgcttt ttgtttccca tgaccgatat ttcatcaatc
    18601 ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag caaagtctat ctaggagact
    18661 atgattatta tttagagaaa aagcaggaag aagaagaaat cgccgaattg ttggcacaag
    18721 atgagccaga atctgctcct gctcccacga acaaatctga ttattaccaa agcaaagaac
    18781 agcaaaagct tatccgtagc cttcaacgaa aaattactca ggttgaagaa gaaatggcgc
    18841 ggatcgatga atggatcgat cagttggaac aagaaatggt cgcgccagaa aacctggaag
    18901 atcacgtaaa actcaatgag ctgaaccaag atttagaagc tgctagacaa gatcaagaaa
    18961 caaaattgac ggaatgggaa gaactaagct tggaattaga agaatatgaa aattaacgaa
    19021 aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc tttttttatt ttatcaaaca
    19081 aaaattccat atttaccttt ttcaccaata agaagaccaa ttactagtca ttctcactaa
    19141 agcttctaaa taatagtaat caccccaaat acaaggtgta tttaccccat tatttttggg
    19201 cttattataa acagactgtg ttaaaaagcc atcttcttct tccgttgtag catatctttc
    19261 tttcaattgt cgcataattt tccaaacagc agactggtat aagttctttt ctgttttgtc
    19321 taatagacct gattctatta attctaataa tccacaaacc atgatcacca tactagatgt
    19381 atctttttct tctctgctag aatcatcaaa gatcaaatcc caataacaaa tggtatcttc
    19441 tggcaaccga ttcataaaat aattggctaa tattttgctt aaatcaaaat atttttgttc
    19501 tttcaaataa cgataactta atgcaaaacc gtaaatcccc catgcttggc ctctagccca
    19561 acaggaatta tcactataac cttgatgcgt acttccatat cttggttttc catctttgac
    19621 atccatataa aatgtatgat aagttgaacc atcttctctt actaaataat ctgctgcatt
    19681 ttgaatatga gcctgagcag cttttttata tttttgattt ccagtttctt cactcgccca
    19741 atacaataat ggaagattca tgcaacaatc catgatcatt ctaccttgtt gtgaaggatc
    19801 actaggatct ccccaagctt gtataactcc tgccttctcc caaaaacgct gaagtaatgt
    19861 ttctgcagca tcaattgaag cttccttcat ttttcgattt cccgttattt tataatctgc
    19921 tacggttgat aaagaataca aaaaaccaag atcgtgatga ttagtgataa tttgacagtc
    19981 aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt tgttgaaata aaggatcacc
    20041 cgtcttctca aataacaacc aattaattcc tgtccaaaat ccttctgtcc aaccaatatt
    20101 gtctcttaat ggataacgtc cttttatagg acactcgctt gggaattgat actgactgtt
    20161 actgatacgt ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct cattattcca
    20221 ttctttttcc attaattctt tataacattc atttatccat ttttctggat gtattatctc
    20281 ttcaaacatc actttatcaa gcatttcccc accagccttt agcataattt tagtcttttt
    20341 ttattgtttt tattttggat agttagcccg gttgtcccat caaaatcaat tttgggacga
    20401 ttgttccata cttctaaact ataatcccga tcttgatgag caaccactcc tgttccgagc
    20461 caatgggttc cttttccaaa tgtaccctct aatccaggaa taaaagtgtt ttcccatgtc
    20521 atcaagtttg tattgggcaa acccataata gctgtggatt ttcgtttaat agtatcgtca
    20581 cttaaacaga taatacctga aaagccagct tcactcaccc tatagttttc cttgtcttct
    20641 tctatgatta ttccttcttt ctcaggagaa cattcaatag caaaccctgt ctcatataga
    20701 acatattctg tatctaattc tactttgtgt acgcgaatct gccagccttc aaaaggaata
    20761 agccaagttc tgatttttgc ctgtaaagta ggttcccatg tagaggcaag tacctctttc
    20821 ttacatacaa tgtctcgtac attatttcga acgatgaact gtccataggt tttgatgctc
    20881 tcattcttaa aactggtcgt gatatatgtg tccgctcgct gaatccctag cattgaatcc
    20941 attgccattt ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa ttgactagaa
    21001 taagcaaatt ttaaatattt ttcattggtc aatgtatgga aatttggccc taattgtccc
    21061 gcattcaaaa aaaatgtatg tccattatca tgttgtatga gcaaattggc ttctgaaaga
    21121 agtttagttc cttcacgaat aggcataggc atttctttag ccgtccaata ctcatggtct
    21181 tcaggtagcg ccaacaataa aaaaatctta ttcagccata atggagataa cgtactatta
    21241 tagggttcag tcaatgtcaa ttgagggtag gtataaccaa tattcaggat acctttttca
    21301 tcaaatattt ctttttgcat ccaccatctt atattccgat tgactaaccc cttgatttca
    21361 cctaatttaa aaggcaaaag atcggcccac aacatagctg accaaaagct tgaaactgca
    21421 aaacgataaa tcatgcttcg accaaaaggc acatttgcac catcttccgc aaaaaaatga
    21481 atatagtctc tagaaaaatc aatagctcgg ttcttatata tctggctaat gggcttttct
    21541 ggttctagta cgctataaat caaaccataa taatgaaatg caaaaggatt ataataatct
    21601 tggcgacctc ttgaactgtc tcgataccag ccattttgaa gataacaatt ttctatatta
    21661 tcaaatgctt ctttcagtgc tgattcttta actggataac caaatttttg gataattgtt
    21721 ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac attcatattc aaaaatttgc
    21781 gagaaccaat gaataatctg gcttttttct cctgtatcta attttttcca taataattgt
    21841 tcatgatgat ataacgctaa taacattgcg ggcatttcta ccatacgctg gtctctttca
    21901 tgtatttctc cccaataatc gggatgggaa ggatctgttc cagcttttat tcctgataga
    21961 attaatttca accatcgttc atctaattgg tcaattgcgg gccccgctcc ccataaaacc
    22021 cgagagaatc cttccattcc tgcaatttta tttccatatc ctaccccttg atgtatataa
    22081 tcaatttgtg cgccacttgc tcgataaaac ggctggagtg gttgatttat ctttttaaat
    22141 aagtcaaagt aatcctgtct ggtccttaat agtggtactg gatgtttcat tgtattttct
    22201 cctcttttta ctaccaaata gctaattccg actgttctaa tcgaagtaag ccttcaataa
    22261 aaaaataatc agaatagatc agtggcacat tataagtcag ttctctatgg tatgcttctg
    22321 ctccgcctcc aatgatccca tcttcatcca tattccaatt tgcatagtgc ttttctgtct
    22381 tttggagtat attgatagca gcttcatgat acatttttga tcgtacagag gaaacatatc
    22441 ctgctaattc cagaagaccg catgcagcgc atactccagc actggtatca atattcgaat
    22501 cttttacagc aaaatcaatg agaggaatat aaccagtttt ttcgattttc tgaataaaat
    22561 ttactgctat cttttctgct atagctagat agtacggatt ttttgtgtaa cgataactta
    22621 acgcgaatcc atataaagcc catgcatttc ctctgctcca acaagaattc tcattgtatc
    22681 cttgcccgcc aagtaaccga atatatttcc ctgattctgc atcaaattct gcgatatgcc
    22741 cggttgatcc atctttcctc actagatact tagctgttgt atctgcatgc tgccgagcaa
    22801 ttttagagaa cctcggatca ccagtttccc tcgaagccca atacagtaat ggtaaattca
    22861 tcatactatc aataatcatc caaccaggtt tatggtcatt ccatgctact aaatagttcc
    22921 ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg taaccctagt tgtcgactat
    22981 gtttgttttg agactgttga taatgtgcaa tagccgatgg taagaataaa aaacctacat
    23041 catgatgaac gtctgaaaaa tggtacaggg cagattctaa taattgttct tgttgtattg
    23101 ctgcctcttt gaacttttct cgttttgtct tgtgaaagag ctgccataaa ataccactcc
    23161 aaaaaccatt ggtccaccag tcgattcctc tgtcctttat tacgtctgta tattttccgt
    23221 tgtaagaaat atatggaatt ttattcccca ttctgtctaa ttccgcatct atcttttcag
    23281 aagctacatt caaaattttt cttgaccatt ccttcgtctg ttcattcatg gatatcccgc
    23341 cattctttta atttttatga atgtggagtt gctcctagaa cgaactagat ggttaacatt
    23401 cgttattttt tttacttatt tgcagctcca atgtcattct ccttaaattt agactgcgcc
    23461 cacacataaa tcaagaccat tgggatcact gaagttgcta aaattagcat aattgcgcca
    23521 taattaattg gctcacctgt actagaaacc gtcatatttt tgataattaa gggaaccgta
    23581 tatttatctt tatctgaaag catgactaat ggaaatagat attgattcca acttcccata
    23641 aagataagta aaccactagt aataatcgta ggtgataaaa tcggtacagc gattttcgta
    23701 aaaatcaccc actcattggc accatcgatt ctggcagcct ccatcagtgc tgtaggaaat
    23761 tgactgctat attgtcgcat cataaacact gaagttgtac tggcaatcga agggatgatc
    23821 acacctgcat aagtattcga taatcctaaa cgactcataa tgtaaaataa aggaatcaat
    23881 gtggtaaaaa aaggcaccat cctagatagc ataactgtat tgaacaacag tttattcccc
    23941 ataaaacgat actttgctaa tgcataaccc gccattgtag aactggctgt agctaaaact
    24001 gtactgatta cagcaataac aatcgaattc caaataatca agggataatt atatctcgcc
    24061 atgatggctt gccagttttt taatagcata tccccaaact tgaaattgat tccgccactt
    24121 aaaatttctt gattactttg cgttgaagct acaatcatat ataaatatgg catcaaaaat
    24181 attaaggcac caaacacaat aatgacgtac gtaaaaattc tgctttgtat cttttgttta
    24241 gtcattttta tcacctccga atttaaattg aatagccgat aatataacag atacaaacac
    24301 catcgtccag gcaatagcag aggcttttcc aaaatcaacc atttcaaaac ttgttttata
    24361 taaattgact cctacgacca aagctgaatt ttctggtcca ttggaagtcc caaagaatat
    24421 atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg ccattaaaat
    24481 gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg atgctccatc
    24541 tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta aaaataaaat
    24601 agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc gtatccaaaa
    24661 aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc cattgaataa
    24721 cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg atgtaatggt
    24781 tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg aattcaataa
    24841 aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca tgataatcaa
    24901 aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat aattgtcaaa
    24961 tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg agataatgat
    25021 gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc cataagcact
    25081 ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct tgcttggtaa
    25141 cgaagattgc atcatcgtta agtcctccta atatttttta atcaccagtt aattttacgc
    25201 cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa tcacctgttt
    25261 gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa gtggctggta
    25321 aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat ttttgtcctc
    25381 cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag ttagatgtag
    25441 ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata aatgtaagaa
    25501 attgttgtgc cacttgtgga tttttggcat ctttagggac ataaaaactt gatcctcctg
    25561 atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc atgcgccatt
    25621 tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg ccagcaacaa
    25681 acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa tgtacaatat
    25741 ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta gtattcagac
    25801 ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg ttgataaaca
    25861 attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt tcttttaatg
    25921 cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct acacctattt
    25981 cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta gcccaaggaa
    26041 taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca taaaactgat
    26101 cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct tgaaattttt
    26161 ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata tcttcgtgtg
    26221 ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca ttcacaattt
    26281 ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt tcaacatagt
    26341 ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt atcattggat
    26401 ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag ctgaataata
    26461 gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct atcccttctt
    26521 tcttttcaag ttcttatata aaacgctttc acttatgtgt attaacggaa ttagtaacac
    26581 aaatatttat ataaaaaaat attttacttg tcgttttggc ttttcatttt tgttataata
    26641 gtcattaatt ataaatagaa agctttttat ataaaaagag aaaggaaagt ggttcttttg
    26701 ttaacaaaca accaatcctc caatcttgtc aaaaaaacac taacctattc catagatacc
    26761 catcttcatt tatctgctgc tccctatttt cgactactaa taaatgaaag cgctaattta
    26821 tatgctgaat ttgcacataa atctatagaa ctagataatc cttccctttt attcattgat
    26881 aaacagcaag aagtccaaat cttttctact caaaaggaaa cacaaacttt tatcattatc
    26941 cagttttatt ttgatgagca attattagca catattacgg gacaatcttt ttctatagat
    27001 caagaaataa catctatcat tacttttctg aaaaatgaac agttcaaaat tgattatcta
    27061 aaacatagta agccagctat ttcctatgat caggaaatca attctttttt catactatgc
    27121 caacagatga gcattcattt gctttcctta ttagttactt attgtctgca aaaaattcaa
    27181 ttgtcttcct taccaaggca ttatcaacag gaacttgctt tattgacaat accttcaaat
    27241 gagaaagtca ttcctaaaaa aaagcatatt ctttattcag atgaattagt tgatccaatc
    27301 atacgatatt tagaagaaca tctttcagaa aattttttgc ttgaacaatt agcccaaatt
    27361 ttttttatca gtccagccag cttaaaaaaa cattttagaa aagtgaatgg aaaaagtgtt
    27421 attagctatt ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct agaaaatgaa
    27481 ttaacctata cagaaattgc agatatatta ggatttcatt cgatccatca tttttcttca
    27541 gcttttaaaa aatatacagg gttatcccct tcaaagtatt atctatcctt aaaatagaaa
    27601 aaaattttta ggttttatcc acaaattatt tctctatatt ttctgtctat tcaaaataaa
    27661 caataatttt ccggcacaat tatttgctgt tttttaccat aacatagtac gatcaacgta
    27721 gaaaaggaga tgtttttatg aaagttgttg tcattggcgg tgtagctggc ggtccttcat
    27781 ttgccactcg tttccgtcga ttgaatgaag cgcacgaaat cattatctat gaacgcggag
    27841 aaaatatttc ttatgcaagt tgtgctttgc cttattattt aggaggtgtg atcactgacc
    27901 gtgactcgct gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac atcgatgtct
    27961 ttactaaaca cgaagtaaca gcaatcgatc cttctactaa gcgattaaca gttaaagacc
    28021 tatccacaaa tgaagaaata aaaacagatt acgataagtt gatcatctct tctggtgcta
    28081 gaccagatta tccggatatt cccggagttt ttgaagcaga aaacggcttt gtcctccgta
    28141 gtgtgacgga tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca caacatgtcg
    28201 tcattcttgg tgcaggtgtt atgggtctgg aattagctga gaatttcaag catcgcggct
    28261 tagacgtgac tttaatcgat caattgccac aagtcgcttt cccttatgat ccagaaattg
    28321 ctaatttagt ttatgacaaa ttgctgaaag aaggattagc cgttcattta gaaacaagag
    28381 ttactgagat ccgtgataaa ggtcgagaaa tagtattatc agatggctcc gtcctttctg
    28441 ctgatatgct aatttttgct gttggtgttt ctccgaagaa tgaagtggtg aaagcaaccg
    28501 gcatacaatt atcagataca ggtcagatca ttgttgatga ccagttacaa accaatctgc
    28561 cggacatcta tgcgattggc gatattatcg aaacgactag tgtagtgact ggtcagccga
    28621 tccaaagtat gctttccagt gcggccaatc gtcaaggaca tatgctggca gatattttaa
    28681 atggtacgcc tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa atctttgatc
    28741 atacagcaag ttatgcggga atgacagaac atgaattaaa agcatcaggc ataacaaatt
    28801 ataaaactgt ttttatcact ccctttgacc atgcctattt ctatccagaa gctacaagat
    28861 taaatctaaa gctgattttt gatgcagata gcggtcgtat tttaggtgga caagcatttg
    28921 gagaaaaagg tgtcgataaa cggatgggag aactttctgt agcaatcacc ggaaacttga
    28981 cagtctttga tttgcctgat ttggagttgc cttactctcc accgtattct actacccgtg
    29041 acccgttgaa tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc gtagaaacga
    29101 tcaaagcaag tgatataccc gaaaacgatt tgaaagaagc gttcttttta gatatacgtg
    29161 aacctaataa agcaccttct ggcagtatct cggcaacaaa aaatattcca atgaatgagt
    29221 tacgagatcg aatcaatgaa atcccaaaag ataaaaaagt ttacattact ttcagaagag
    29281 gattgaatac ttatacttct gcccgaatct tggcaggttt gggtatcaaa gcgattttga
    29341 ttgaagaata aaaaaacgac cgcggtgggt agccgcggtc taggagttat ttttacttga
    29401 ggagtaaaaa tgaaaaagta tatagggttg ttggttggta tgtatataga atagaagata
    29461 aatgtgaaga aattgtgcat tatttcttca catttattag gttatagttt aagaaagttt
    29521 aaacattgcg caatttaatt attttagctg tttcttttca taaaaaaaca tgttgtgact
    29581 ctgagacaaa tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa tattacgagg
    29641 catcatatta atctatcagc actagtacct aaactagcat ccctttcgaa tattttttct
    29701 tatataataa agtttgcttt cactcctcta atctcaccaa atcgactggc ttgcgttgtg
    29761 tgatactagg aactgattgg tttagaacga cttttctatt gtaatcatat ttttcgccat
    29821 cctcgttgtc ttctaatcct tcaatgaatg atccctaatg tgtgtattaa ataatttaat
    29881 ttttttgttc ctatttattt a
//
LOCUS       DACDNO010000033        29783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDNO010000033 DACDNO010000000
VERSION     DACDNO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.033"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(594..1985)
                     /locus_tag="IWI22_001527"
     CDS             complement(594..1985)
                     /locus_tag="IWI22_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9168922.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAVAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2198..2584)
                     /locus_tag="IWI22_001528"
     CDS             complement(2198..2584)
                     /locus_tag="IWI22_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9168923.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2640..3263)
                     /locus_tag="IWI22_001529"
     CDS             complement(2640..3263)
                     /locus_tag="IWI22_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9168924.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3327..3995)
                     /locus_tag="IWI22_001530"
     CDS             complement(3327..3995)
                     /locus_tag="IWI22_001530"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9168925.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4007..4447)
                     /locus_tag="IWI22_001531"
     CDS             complement(4007..4447)
                     /locus_tag="IWI22_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168926.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4506..5177)
                     /locus_tag="IWI22_001532"
     CDS             complement(4506..5177)
                     /locus_tag="IWI22_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168927.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFLPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(5362..5760)
                     /locus_tag="IWI22_001533"
     CDS             complement(5362..5760)
                     /locus_tag="IWI22_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9168928.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5757..6530)
                     /locus_tag="IWI22_001534"
     CDS             complement(5757..6530)
                     /locus_tag="IWI22_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9168929.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6527..7210)
                     /locus_tag="IWI22_001535"
     CDS             complement(6527..7210)
                     /locus_tag="IWI22_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9168930.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7377..8264)
                     /locus_tag="IWI22_001536"
     CDS             complement(7377..8264)
                     /locus_tag="IWI22_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9168931.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8261..9130)
                     /locus_tag="IWI22_001537"
     CDS             complement(8261..9130)
                     /locus_tag="IWI22_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9168932.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9267..10553)
                     /locus_tag="IWI22_001538"
     CDS             complement(9267..10553)
                     /locus_tag="IWI22_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9168933.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10826..11404
                     /locus_tag="IWI22_001539"
     CDS             10826..11404
                     /locus_tag="IWI22_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9168934.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11404..12333
                     /locus_tag="IWI22_001540"
     CDS             11404..12333
                     /locus_tag="IWI22_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9168935.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12330..14066
                     /locus_tag="IWI22_001541"
     CDS             12330..14066
                     /locus_tag="IWI22_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9168936.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14129..14965)
                     /gene="pheA"
                     /locus_tag="IWI22_001542"
     CDS             complement(14129..14965)
                     /gene="pheA"
                     /locus_tag="IWI22_001542"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9168937.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14962..15471)
                     /locus_tag="IWI22_001543"
     CDS             complement(14962..15471)
                     /locus_tag="IWI22_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9168938.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15464..16759)
                     /gene="aroA"
                     /locus_tag="IWI22_001544"
     CDS             complement(15464..16759)
                     /gene="aroA"
                     /locus_tag="IWI22_001544"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9168939.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16772..17863)
                     /locus_tag="IWI22_001545"
     CDS             complement(16772..17863)
                     /locus_tag="IWI22_001545"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9168940.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17880..19046)
                     /gene="aroC"
                     /locus_tag="IWI22_001546"
     CDS             complement(17880..19046)
                     /gene="aroC"
                     /locus_tag="IWI22_001546"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9168941.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19048..20112)
                     /gene="aroB"
                     /locus_tag="IWI22_001547"
     CDS             complement(19048..20112)
                     /gene="aroB"
                     /locus_tag="IWI22_001547"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9168942.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20145..21167)
                     /gene="aroF"
                     /locus_tag="IWI22_001548"
     CDS             complement(20145..21167)
                     /gene="aroF"
                     /locus_tag="IWI22_001548"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9168943.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21826..22800)
                     /locus_tag="IWI22_001549"
     CDS             complement(21826..22800)
                     /locus_tag="IWI22_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9168944.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22781..24664)
                     /locus_tag="IWI22_001550"
     CDS             complement(22781..24664)
                     /locus_tag="IWI22_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9168945.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24682..26907)
                     /locus_tag="IWI22_001551"
     CDS             complement(24682..26907)
                     /locus_tag="IWI22_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9168946.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            27022..28056
                     /locus_tag="IWI22_001552"
     CDS             27022..28056
                     /locus_tag="IWI22_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9168947.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(28123..29136)
                     /locus_tag="IWI22_001553"
     CDS             complement(28123..29136)
                     /locus_tag="IWI22_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9168948.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(29214..29402)
                     /locus_tag="IWI22_001554"
     CDS             complement(29214..29402)
                     /locus_tag="IWI22_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9168949.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         8582 a         6437 c         5257 g         9507 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgtaaaat atgaggaact atttttgttt
      421 ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttattc acggtgtttc
      481 agaagtagct tctcggaaat aagccgaaat ttcacaaaaa tttgagaaac tatttttagg
      541 aaacttcttc ttatttctta aagctgacca cttctatcac aacctctttt tgactatgca
      601 ttcacatcga tcactttaga atcatcattg atgacatcgc gatctaaatc tttgatgcgt
      661 gcacttgtca cgataccagc taccatgcta ccgctgacgt ttactgctgt acgtgccata
      721 tcgattaatg gttcaacaga gataactaac ccaacgattg ctactggcaa gttcattgcc
      781 cctaaaacga tcagtgaagc aaatgttgcg cccccaccga ctccagctac accaaaagaa
      841 ctgattgtta caattgccac aagcatcaaa ataaattcca aactaaagac attcactcca
      901 gctgctggtg cgacgatcgt tgccaacata gctggataaa cgccagcaca gccgttttgt
      961 ccaatagaca aaccaaaact tgctgagaaa ttagctgttg catcatctac tcctaaagct
     1021 tttgtctgag tctcgatatt caatggcaaa gctccagcac ttgaacgtga agtaaacgca
     1081 aagctcaata ccggaaaggc ttttttgaaa tagttgattg gattgacttt tgcaccaatc
     1141 aaaatcagtg agtggacaat caaaacagca agcaatgcta cgtaagaagc aacgacaaat
     1201 ttacctaaat tgacgatagc tgcaaaatca cttgttgcca aaacgtttgt catcaaagca
     1261 aatactccat aaggtgtcaa acgcaaaaca agcgtcacga tccgcataat gattttatac
     1321 agactgtcga ctaaatctga aaagaatgct gcttcttttg gcgcttttct tttcacgcct
     1381 agataagcga ttccgacaaa tgccgcgaaa ataacgactc caatcgtact tgttgcacga
     1441 ctacctgcta aatcagcaaa aacgttttta gggataaagg ataaaatctg ctgtggaatc
     1501 gataagtttt caactgtttc ctgacgtgtt gccagttcag agattcgtgc tgtttctgca
     1561 gctccttgtg tgaatttagc tccttggagt ccgaacagca ttgttgaacc aattccgatc
     1621 aatgctgcaa cagctgttgt tccaagtaaa gaagtcaata cagtcgcact aattttccct
     1681 agcttttcag aaactttcat ttttgtgaat gcaccaatga tcgaaataaa cactaaaggc
     1741 ataactagca tttgcaagaa tgatacatat ccttgaccta caatcccgat ccattccatt
     1801 gattgagtaa cgactttgtc gtctacacca aagatcaatt gaagcagacc gccaaagacg
     1861 ataccaactc ctaatgctgt aaagacgcgt gttgaaaatt ttgtatggcg cttttgcata
     1921 cgatagaaca catataataa tgccgcaaaa actagaacca ctaaaactgt gatgagtgtt
     1981 gtcatttttc tcctcctcat attattaatg acaagtccat cgaaggaagt aaaggttccc
     2041 gagaatgatt cttcaaccat cctcctgaaa aaattttttt cagtgacttt tatattttag
     2101 acgattttta tcgaaatgac attctttttg cttacaatta gtaataaaaa aacatacctg
     2161 tgatagcttt aatctatcgc aggtatgttt ttactgtcta ttcttgtttt tctgattgaa
     2221 aattcaattt taccccaggc tgatctaagg cgatctctgt cacctcataa gacattcggc
     2281 gtacgatatc gaataacggt gctactagct cgtccacttc ttcttgtgtg acatcatttt
     2341 gaccttcaat tttccgattg aggatatgat tgatctgact cacacttcca gataaaacat
     2401 atgtatcaaa cactaaacgg aactctaagc gtgctcctac gatgctatgt tctttcggat
     2461 agttttcgat cgtttctttt aaaggcgaga agcctacttt caaatctgtt tctacttctt
     2521 ggttcggcaa tctcatatca taatgaaatg cttctacaac ttctttttgt cttctgattt
     2581 ccatataatt ttatcactcc tttatgaaaa ttataacata tctcctttga aaggggatac
     2641 tactttttaa tgatgctctg caaggtaagg atttcattca gatttcctac cacataatct
     2701 gccgggatct cacccagaaa atgatcgatg tcgtaaagac aagtagcgac tcctgcattt
     2761 ttccctgctt cgatatctaa acgacgatcg ccgatcatca tcgtgtctgt tctttctaaa
     2821 tgaaaagtat cgatcaaata atttaaagat gctggatcag gtttacgagg aaagtcctga
     2881 tcgatcccta ctacttcctc aatcagatgt gccaatcgat gttcttttaa caggccccat
     2941 gttgattctg tcaaacggtg ggtcaggatg aaatgacgac cgccgttgtc ttttagcatt
     3001 tccagtactt gtttcgtttc tggaaatgga cgggaaactt ccgtactttc tttttcatat
     3061 cggtgaaata attcattaaa ctctctggca ttcaagtttg attcattgat caaataacta
     3121 gtagaatact ttttcatttt gaagtagatt tcttttttgt ccatactgat accaaaatct
     3181 tttagtgctt gccatgcacc atctaccatt gcgggatacg tatcaaacaa tgtaccatca
     3241 aagtcccata tataattttt catgttcctt ttgttccatt cctttgcttc ttttttctat
     3301 ctctcacttt tagataaagg tgttctttaa agtatttttt tgtccagaat tgtcaaatca
     3361 tctgctaatg tatactggat tggttgtgca tttgggatat cgatcgtatc catttgatct
     3421 tcaggaacat cttctaaata ttttgtcagt gctcggatgc tattaccatg acccacaact
     3481 agtaaatctt gccgatcttt tagttttggt gctagatgat cttgccataa gggaatgatc
     3541 cgattcactg tcatttccag actttctccg tatgggatca aatgcggatc caagtgatca
     3601 tatctgcggt caaatctatt ttctgcggtt aacggcggaa cttccgtata tcctctgcgc
     3661 catgttttta cttgatcttt tccgtatatt tctttcatct tatctttgtt tttacccaca
     3721 agatccccat aatgccgttc atttagacgc catgttttta ttataggaag ccataactga
     3781 cccatttctt ctagtgcgac ttgtccggta agaatcgcac gtttcaaaac agaggtataa
     3841 acggcatcga attgaagtcc ggcttcaagc attttttgac cggcaactgc tgcttgttct
     3901 tttccttttt ctgttaaggc gacatctagc catcctgtcc agtaattttc aaaatttgct
     3961 tcgctttcgc catgacgtaa taatactaat ttcattcttt tctcccttaa acattcgttt
     4021 cgaattttat accacatgtt ttgcatgtca cacgtattcg tccttttcct ttaggtgcac
     4081 gctgcttttg atgacaattc gggcactcaa aaaaagtata actttttcga tccattttga
     4141 cttgcttttt gttttttatc tgatgatatt tttctttgac tctatcaaga tatttcaaat
     4201 aacgttgatt ctcatttagt ctggggtaga ttttttttga taggaaacga aatatcatcc
     4261 atccgaaaaa gacgatgaac aacaattgtc cgattctata gggcaaccaa cgacttagca
     4321 aactaaagac aagcgcagcg atcaatagac cgcgattcaa ctgatcaaat ttcgcatatc
     4381 ttccctgcat aagtttgtga tagttctgct gaaatcgtat tagcctttct aaccatgttt
     4441 gcatcatatt atctcctcca tcatctaata tttattatga caaaaaaatc caaaaagaaa
     4501 aagccttaat tggccttttc cattgcaact tggtgaagga cattccgatt tctggtaaag
     4561 tcaatcacaa tcactttctt tttatcttct ctgttgacta ctcgaaatgt gtccgtcaat
     4621 ggaagaaaca gcttcttaga tggtagctgt tgcatacgta taaaacgatg gatcatcgta
     4681 atcaattgag gaaattgaat agacgtgtga agcagacctg gatatttttt caacagctgt
     4741 gtcgttttga tcagattccg ttttttactg atcactttta aaaagctttt tagaatcttt
     4801 tgctgacgtc taaaaataga aaaagaattt tcgtcatcta tttgataacg catttgtttt
     4861 tgcagttctg ctgcagtcag atggatcttc ccatcgtttg tatcgatcat taaaccttct
     4921 ggaaacagtt caaccactgc tcgatcccaa gattcaagag aaagctcgat ataatctttt
     4981 actggtaatt ccaattctac cttaaaaaag tgcaaaacag ccgacagccc ttctgcttga
     5041 tagatttctc ctaatgttag cccctctaga tggaactctg gtaaaaaatg cagcaatgtt
     5101 ttattttcct gcaattgaac atatagaaat gtagatcgct caatcagagt atcttttaaa
     5161 acaactaaaa aattcatttt tctccccctt ttttcatcat ttttaaaata gcatattttg
     5221 aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc ataagtattc
     5281 acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt gtttgtgttt
     5341 atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat tttcatctaa
     5401 aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac caatgacttt
     5461 gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga tgccttgacc
     5521 aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca caatatcttt
     5581 ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag aacctcttcc
     5641 atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa catcttcgcc
     5701 cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa gatctttcat
     5761 ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc atcttttcgt
     5821 ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat cattgcaacc
     5881 acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat tgccacgctc
     5941 attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt atcaaaagcc
     6001 attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc gtatcctggg
     6061 atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa tcctactgct
     6121 gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct cccctcgctc
     6181 aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc tactaagaac
     6241 cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc aatagcactg
     6301 cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa aaggatcaac
     6361 ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt gcgcatacga
     6421 aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc caaaatgact
     6481 tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat tggaagatca
     6541 cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc attccaaaac
     6601 tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca agggccaaga
     6661 taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca atcggcataa
     6721 attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata gccatcaatt
     6781 gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat actccagtgc
     6841 cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat atatcatata
     6901 cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc ccgatagcaa
     6961 atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc cctactagat
     7021 ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt ccgatcatca
     7081 tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt gcaaaaacaa
     7141 acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat
     7201 tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc
     7261 gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca
     7321 aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat
     7381 tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca
     7441 gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa
     7501 gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact
     7561 aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag
     7621 gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct
     7681 aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag
     7741 atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa
     7801 accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga
     7861 accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc
     7921 tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg
     7981 atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga
     8041 aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta
     8101 ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt
     8161 aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg
     8221 acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta
     8281 ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg
     8341 ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg
     8401 tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca
     8461 atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg
     8521 tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct
     8581 ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc
     8641 aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg
     8701 gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata
     8761 gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg
     8821 ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg
     8881 ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt
     8941 ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat
     9001 tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca
     9061 tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt
     9121 tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa
     9181 gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc
     9241 gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt
     9301 gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct
     9361 acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc
     9421 catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa
     9481 gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg
     9541 cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct
     9601 ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt
     9661 tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct
     9721 gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat
     9781 tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa
     9841 agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc
     9901 tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca
     9961 aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct
    10021 acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct
    10081 gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat
    10141 attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca
    10201 atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa
    10261 tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc
    10321 agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc
    10381 tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag
    10441 acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa
    10501 cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc
    10561 cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa
    10621 aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca
    10681 aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt
    10741 ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa
    10801 gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca
    10861 gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat
    10921 agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat
    10981 cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa
    11041 acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt
    11101 taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga
    11161 tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag
    11221 cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca
    11281 aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc
    11341 gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca
    11401 ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc
    11461 ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt
    11521 caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc
    11581 aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga
    11641 aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca
    11701 agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag
    11761 aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca
    11821 atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa
    11881 ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat
    11941 ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa
    12001 aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa
    12061 gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc
    12121 atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt
    12181 aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc
    12241 ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct
    12301 tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct
    12361 ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt
    12421 tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg
    12481 tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat
    12541 tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac
    12601 atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa
    12661 tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg
    12721 gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga
    12781 gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc
    12841 gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg
    12901 tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg
    12961 tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat
    13021 caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg
    13081 aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta
    13141 cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga
    13201 ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg
    13261 atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg
    13321 gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga
    13381 tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga
    13441 agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata
    13501 atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag
    13561 gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc
    13621 cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg
    13681 gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga
    13741 atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc
    13801 aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg
    13861 ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa
    13921 tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa
    13981 cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta
    14041 aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt
    14101 taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg
    14161 gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct
    14221 ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc
    14281 tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg
    14341 ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt
    14401 ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct
    14461 ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg
    14521 tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg
    14581 cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact
    14641 gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat
    14701 tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat
    14761 gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa
    14821 cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat
    14881 acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt
    14941 ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat
    15001 cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga
    15061 aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg
    15121 aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta
    15181 aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc
    15241 accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt
    15301 tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc
    15361 ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc
    15421 gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc
    15481 ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt
    15541 cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac
    15601 cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga
    15661 tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca
    15721 ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga
    15781 ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa
    15841 tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg
    15901 attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat
    15961 ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg
    16021 gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta
    16081 tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga
    16141 tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa
    16201 cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg
    16261 cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga
    16321 ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt
    16381 ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa
    16441 aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt
    16501 caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct
    16561 cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat
    16621 cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga
    16681 tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta
    16741 tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat
    16801 tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca
    16861 gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg
    16921 cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa
    16981 ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt
    17041 cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg
    17101 ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc
    17161 tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa
    17221 agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat
    17281 tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat
    17341 gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt
    17401 tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata
    17461 gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga
    17521 caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat
    17581 ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg
    17641 ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat
    17701 cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac
    17761 tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag
    17821 caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt
    17881 tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa
    17941 tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca
    18001 acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt
    18061 tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg
    18121 accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta
    18181 ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag
    18241 cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata
    18301 ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct
    18361 aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt
    18421 ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt
    18481 acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct
    18541 gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga
    18601 attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt
    18661 tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt
    18721 cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca
    18781 gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa
    18841 ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc
    18901 atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct
    18961 tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg
    19021 gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc
    19081 tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg
    19141 gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag
    19201 atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc
    19261 agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac
    19321 tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt
    19381 atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt
    19441 tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc
    19501 gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat
    19561 gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc
    19621 aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt
    19681 gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg
    19741 aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat
    19801 tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc
    19861 gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag
    19921 gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc
    19981 aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga
    20041 tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg
    20101 tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga
    20161 atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt
    20221 tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg
    20281 atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa
    20341 attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat
    20401 gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt
    20461 tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat
    20521 tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca
    20581 gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac
    20641 tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat
    20701 tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat
    20761 ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc
    20821 attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc
    20881 acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg
    20941 aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca
    21001 tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact
    21061 cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact
    21121 ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct
    21181 ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca
    21241 ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt
    21301 atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca
    21361 tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc
    21421 tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt
    21481 gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt
    21541 cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta
    21601 cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt
    21661 ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca
    21721 tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag
    21781 ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa
    21841 gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca
    21901 gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc
    21961 tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc
    22021 ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt
    22081 atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat
    22141 gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc
    22201 ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa
    22261 aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc
    22321 tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga tttggtagga
    22381 aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt
    22441 ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt
    22501 tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca
    22561 cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa
    22621 aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc
    22681 gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc
    22741 gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat
    22801 aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat
    22861 tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt
    22921 ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc
    22981 ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg
    23041 aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa
    23101 ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat
    23161 cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt
    23221 ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag
    23281 accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa
    23341 gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc
    23401 ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg
    23461 cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt
    23521 tgatctttgc attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc
    23581 gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat
    23641 tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat
    23701 actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct
    23761 gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt
    23821 ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca
    23881 gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac
    23941 atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa
    24001 ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg
    24061 cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg
    24121 ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca
    24181 accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta
    24241 gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg
    24301 aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat
    24361 gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa
    24421 tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg
    24481 tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact
    24541 gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat
    24601 gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt
    24661 tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc
    24721 accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg
    24781 cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg
    24841 gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt
    24901 aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata
    24961 atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg
    25021 ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat
    25081 agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc
    25141 tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt
    25201 catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa
    25261 cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc
    25321 agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc
    25381 ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca
    25441 tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg
    25501 ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct
    25561 acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat
    25621 catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat
    25681 ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt
    25741 gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc
    25801 ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc
    25861 ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc
    25921 acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag
    25981 gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct
    26041 gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg
    26101 gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg
    26161 atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa
    26221 tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc
    26281 tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc
    26341 cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat
    26401 cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc
    26461 cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa
    26521 gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa
    26581 gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg
    26641 agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac
    26701 atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac
    26761 tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga
    26821 aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt
    26881 atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac
    26941 ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat
    27001 ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc
    27061 tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact
    27121 actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa
    27181 tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa
    27241 gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg
    27301 catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta
    27361 gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg
    27421 ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat
    27481 atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat
    27541 ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg
    27601 aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat
    27661 cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta
    27721 cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc
    27781 gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa
    27841 gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg
    27901 accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag
    27961 cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt
    28021 agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt
    28081 cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac
    28141 aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta
    28201 ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa
    28261 ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc
    28321 agttatatac gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa
    28381 ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa
    28441 aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc
    28501 gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg
    28561 tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc
    28621 actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc
    28681 taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg
    28741 cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc
    28801 aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag
    28861 aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata
    28921 cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt
    28981 ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg
    29041 tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa
    29101 gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt
    29161 aagcattctg attataccat agtgttattt cctatcaagc tctcctagat gatttaatga
    29221 ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca
    29281 aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga
    29341 tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc
    29401 atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt
    29461 ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac
    29521 attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat
    29581 cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa
    29641 aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag
    29701 aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa
    29761 tcgccaaaat aatgtagact aac
//
LOCUS       DACDNO010000034        29570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDNO010000034 DACDNO010000000
VERSION     DACDNO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.034"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="IWI22_001555"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="IWI22_001555"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9168950.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IWI22_001556"
     CDS             complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IWI22_001556"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9168951.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IWI22_001557"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IWI22_001557"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9168952.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="IWI22_001558"
     CDS             complement(3376..4683)
                     /locus_tag="IWI22_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9168953.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="IWI22_001559"
     CDS             complement(4686..5267)
                     /locus_tag="IWI22_001559"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9168954.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="IWI22_001560"
     CDS             complement(5839..7131)
                     /locus_tag="IWI22_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9168955.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="IWI22_001561"
     CDS             complement(7128..7751)
                     /locus_tag="IWI22_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168956.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="IWI22_001562"
     CDS             complement(7754..8590)
                     /locus_tag="IWI22_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9168957.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="IWI22_001563"
     CDS             complement(8607..9494)
                     /locus_tag="IWI22_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9168958.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="IWI22_001564"
     CDS             complement(9510..10793)
                     /locus_tag="IWI22_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9168959.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="IWI22_001565"
     CDS             complement(11476..12159)
                     /locus_tag="IWI22_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9168960.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="IWI22_001566"
     CDS             complement(12177..12794)
                     /locus_tag="IWI22_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9168961.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="IWI22_001567"
     CDS             complement(12804..13628)
                     /locus_tag="IWI22_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9168962.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="IWI22_001568"
     CDS             complement(13621..14181)
                     /locus_tag="IWI22_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168963.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14354..15310
                     /locus_tag="IWI22_001569"
     CDS             14354..15310
                     /locus_tag="IWI22_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9168964.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15615..16571)
                     /locus_tag="IWI22_001570"
     CDS             complement(15615..16571)
                     /locus_tag="IWI22_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9168965.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16675..17277
                     /locus_tag="IWI22_001571"
     CDS             16675..17277
                     /locus_tag="IWI22_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9168966.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17365..18864)
                     /locus_tag="IWI22_001572"
     CDS             complement(17365..18864)
                     /locus_tag="IWI22_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9168967.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18880..19404)
                     /locus_tag="IWI22_001573"
     CDS             complement(18880..19404)
                     /locus_tag="IWI22_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168968.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19702..20451
                     /locus_tag="IWI22_001574"
     CDS             19702..20451
                     /locus_tag="IWI22_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9168969.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20442..21227
                     /locus_tag="IWI22_001575"
     CDS             20442..21227
                     /locus_tag="IWI22_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9168970.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21196..21912
                     /locus_tag="IWI22_001576"
     CDS             21196..21912
                     /locus_tag="IWI22_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9168971.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22014..23033)
                     /locus_tag="IWI22_001577"
     CDS             complement(22014..23033)
                     /locus_tag="IWI22_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9168972.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23052..24110)
                     /locus_tag="IWI22_001578"
     CDS             complement(23052..24110)
                     /locus_tag="IWI22_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9168973.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24331..25479)
                     /gene="nagA"
                     /locus_tag="IWI22_001579"
     CDS             complement(24331..25479)
                     /gene="nagA"
                     /locus_tag="IWI22_001579"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9168974.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25555..26391)
                     /locus_tag="IWI22_001580"
     CDS             complement(25555..26391)
                     /locus_tag="IWI22_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9168975.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26575..27792)
                     /locus_tag="IWI22_001581"
     CDS             complement(26575..27792)
                     /locus_tag="IWI22_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9168976.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28175..28258
                     /locus_tag="IWI22_001582"
     tRNA            28175..28258
                     /locus_tag="IWI22_001582"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28209..28211,aa:Leu,seq:gag)
     gene            complement(28498..29496)
                     /locus_tag="IWI22_001583"
     CDS             complement(28498..29496)
                     /locus_tag="IWI22_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9168977.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8842 a         6304 c         5064 g         9360 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttaa tgtttcaagt cctctcctct
    14281 ttattcttcg tgatttttgt tataataata gacagacaag aaactcaatc caactatgaa
    14341 ggagcgtttt cagatgaaaa aatatcaatg gggaatcgtt ggtcttggta ccatcgcaca
    14401 tgagtttgcc gaaagcttca atcaagagac gagcgaacta acagcagttg cttccagaac
    14461 ttcagaaaaa gcggaaaact tcgctcaccg ttacaatatt ccaaaagctt acggaagcta
    14521 tcaagaaatg ttagacgatg ctgaaatcga catcgtgtac attgctgtac caaatagaca
    14581 gcatatcgac catatcttgg cagcattgga agcaggcaaa cacgttctat gtgaaaaagc
    14641 catcacgatg aataaaaaag aactggctga cgctatgcgc ttagcagaag aaaagaattt
    14701 gatcttagca gaagcgatga caatcttcaa catgccactt tatcagcagt tacgctcaat
    14761 aatggataca ggaaaactgg gggctttaaa aatgatccaa gcacctttcg gcagttacaa
    14821 agaaccagac cctaaaaacc ggttcttcaa tccagaatta gctggaggag ctctgttgga
    14881 tatcggtacg tatgctgtat ctttcgctcg tttctttttg agctctcaac ctgaagtagt
    14941 tgcctctaca atggttcctt ttgaaacagg cgtggacgaa caatccgtca ctatcttgcg
    15001 taacaaagaa aatgaacttg ctgctgtcag cttgactttt caggcaaaaa tgccaaaagt
    15061 cggtatcgtt gcctttgaga acggttatgt gacgattgct gattacccgc gtgctgatcg
    15121 tgcggagatc ttatttacag atggtacaaa ggaattcatc gaaagcggga atacatctga
    15181 agcaatgaat tacgaaataa agaatatggt acacatgatt gaaggtgact tgcccaatcg
    15241 ctctctcttc ttgacaaaag atgtcatcga aatcttggat caaatgcaag ttctatggaa
    15301 aaatatgtaa ttttaaaagg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagcc
    15361 aaacaaaaat agctcctcct atttttcgtt tggctggact tattaccgca gaggaaagct
    15421 tttgaacacc gtttaagaaa gaggttgtga gcctaccgtt cagctccgag tcacaaggag
    15481 caatttcaaa aaacagcttc tcatgtttgt tgaatattgt gacttgtgac cgaggagttg
    15541 acaggagaac accgtttaaa gaagaggttg tgaaatcagc tttttgacct gaatattagc
    15601 tgaattcaca acctctattt ttggattttt tgttgggaga attctgcctt gatttcaata
    15661 aaacttttaa aagactgcaa aaactggaac aattctgctc gatcctcgaa ttcagggcga
    15721 ctagtcggca gaggcatttg ccgatacaat acataaactt cttcaatacg tgttaaaatt
    15781 tgtctgccat catttgactc tgaaaatgtt tctgcagtat aaatcaataa accatagata
    15841 tgttttccat aataagctgg ttgctggatt ctctctagat tttccagcat gtctttaatc
    15901 acattggcct gtgctcttct catagagaag taagtttcgt agtatagcgg ctgtctgagc
    15961 cagtagtttt cttgatgttc tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa
    16021 tgttcgcatt tgatctgcac atcttcttta ttttctgata aaagaaattc tgccatttgt
    16081 ctcaaaatat ttcgaaattc tttttcgatc tgtatttggt tttccttcag ctgcttcgta
    16141 cgatctggca tgtaaacatt tgcaagcaaa gcaaatacta atccaatcgc cattaatccc
    16201 gcttcattgc cgatcagctg ccaagacatg ttttcctcca ctaaataatg agtgaccaaa
    16261 accgaattta ctactatccc ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg
    16321 aataaaaaaa gactgaagcc aaaaatagta tacccaacag caaaaaaact gaacagtgct
    16381 gttgccgtag ctaatataaa ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc
    16441 ttggtatttc ctacacttaa taatgcaatg atccctgcag ctggccaata caacagcgat
    16501 aagctacccg ctgtgaccat cgaaaagaag acactgatca ctgttttgac tgttcgcatt
    16561 cctatacgca tttacttccc ccttttttat tttaattata cttgaataat gaaaaactag
    16621 ctatcagaca aaagtttagg tatgatagta aaaagaccaa aaaggagaat ctctatgcaa
    16681 tttggccgtt ttcgactagg tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt
    16741 atcttgtttt ttcatttgac tcaacgagga atcccgatga ttgctgcatt atctgctgtt
    16801 ttttctttgc gtcaggactt gactacaacc gtttcttttg gaaagtctag gatcatcggc
    16861 aatacattag gtggtacatt agctatcatt tatttttttg tcaaaagcta ttttcacaat
    16921 gattttttgg tagaattgat cttgcttcct attctagtta ttattgtgat cgtcgtgtca
    16981 gatggtatta acaataattc cggtatcatt tccgcaatag ctacgttgtt gctgattgcg
    17041 ttgagcatcc ctcaaggcga atccgctttg tatgctatcc aacgtgtact agatactttt
    17101 atagggacat tcattgcaat cggtatcaat ttctttcttc gtcctcctga aacagaaaag
    17161 aaggaagaac tatcagaaga tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca
    17221 aatttacaag agatagaagc taagatccaa aaacaaaaga aaaatagcga ggactaattt
    17281 tcttttgcaa taaaataaaa aaatggagag aagaacttta aaaagtcttc ctccattttt
    17341 gattgaacgg tgaagtgtac tactttattc ttccttcatc gagttttgtt ctgcctcttt
    17401 tggcgcttca tacatattta cttggctagt agtcccacgt gtcgacaaat tctctacgat
    17461 ttccttcaag tcaagtcctg ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt
    17521 tgtggcataa tttgtcactc ggttcgctcc actggtttct cctgaactgc ttcctgtatc
    17581 gacaacagag attttttcga tatttcctag cggctgtgct gcttcgcgca tcaattgcgg
    17641 tagcatatcg ataaccatac tcaagacagc tgcttcgcca tattccttga aggcattggc
    17701 gatcttttgt tttgcttcgg cttctgcttt tccttttgct agtgctgctt cggcttctgc
    17761 ttgtccagct aaacgtgtct tattggcttc cgcttctgcc agtgcttcca cacggaatcg
    17821 ttctgcttcg gcttcagtca cttctttgac tttttgagct tgtgcttctt gttctttagc
    17881 gtaccgatcg gcatctgctt ttttcttcac ttccgaatca tattgctttt cacgacgtgt
    17941 gatttctttt tcttccagtt cgatttgttt ttggcgttct acgattttaa tctgcatttc
    18001 ctgttcgatt acttgttgtt gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt
    18061 tgctttagca acatcttgtt cttgtttgta ggttgctaat ttcagttctt tttcttttaa
    18121 tgattctgcg atctctgttt gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg
    18181 agcttttttg atacgcgttt cttttagagc ttccgcttct gctatatctg catctctttt
    18241 gacttgggcg attcttggtt tccctaaaga gtcaagataa ccgtttttgt ctctgacctc
    18301 tttgatagtg aaagaaacga tgatcaaccc catttttgca aggtctacag aagcgacttc
    18361 ttgtacgctt tgactgaact tatcgcgatt ttgatagatt tcttctacag tcatagaacc
    18421 aagaatcgag cgcaagtgtc cttctaatac ttctcttgct tcattttcca gttcttctct
    18481 tgtcttgcct aaaaactgtt ctgctgctgt agcaatctct tctacagatg aaccgatttt
    18541 gatgatcgaa gtaccatcac acatcaccgg tacgccttgt tccgtataca cttcaggggt
    18601 ggagacgtcc aatttactcg ataatagact gatccgattc gaacgttgaa aaactggcaa
    18661 gacgaatgcg ccacctccac gcacaatttt gattttgtta ttgctttcat caatgtgcac
    18721 atttttgctt cctaaataac ttccactgat gatcaacgct tcatccggtt ttgctgtctg
    18781 gtacttcgct acaaagacga tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc
    18841 gataaaaaca actggtattt ccatttaaca cacgctcctt taagctttta tcatgctttc
    18901 ataaggcatg acataacaga ttctttcctt gatctcaatg atcaatactt tcgttccccg
    18961 ctcagctgtc tgttcttggg ggctgtaaaa acaagctgga cgactaatgg aaccggtaac
    19021 acttttcaac tgaatttctc ccatacctcg tacagggatc ggtgtgatca caacagctac
    19081 ttgtccttcc agagtttttt cagaagacga catcgttgat tcggcatgtt tcatcggcat
    19141 caatatatag aaattaagca aaaataccaa cactatcgat atgcctccac tagcaataaa
    19201 taaaaataaa gatgattgat ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa
    19261 agttaaccaa gggataataa gcatcggatc aagcgggcca tcaaaatcga atacatctcc
    19321 aaaaataatc aataggactg ccaatcctgc acaaatcagc aatgtatacc aataaatcgt
    19381 ctcaagtgcg attcccccta tcatatacac gcctccttcc atttctacgt ctatcatacc
    19441 aatgtttgaa aattctgtat agcttctttt accgactcag tggtgttttt tataggaagg
    19501 ttgtgtttat cgttgacaat ttgttaaatt tttgttttca tgttaaaatt ttcacaataa
    19561 attctttcga ggtagcctat gtttcctttt tttaagaaaa aaccattaaa gctccagaac
    19621 acgatcatcc ttgtcgtttg tttctccaca ttcgtttctt tgattgtttc tgccatattg
    19681 atcaggaatt ttattctttc gatgaaccga tctagtgtgt cagataaaat ctctagcatt
    19741 gctaaaatcg ttgccaatga taaactcatc caaaaaaagc tgcagactgg tgacccgaca
    19801 catgcgattc aaggaataac aaatgaaatc atgattgcat cgaatttaga ttttatcgtt
    19861 gtcatggacc ctgaaggcat tcgatattct cacgcagacc caagttacat cggtcttcct
    19921 ttctcaagtc atgaagatgt gaaaaaagtg attggaggac aagaattttt ctctgaaaaa
    19981 gaaggaatgt tagggttcgg cttccgctat ttcgtcccta tttattatga tcaagagatt
    20041 attggtgcag tttgtgttgg cttaacaaac aacacgattg acaatcaagt caaagatgga
    20101 caaaaaatga ttgttttttc attgattatt ggacttagcg taggcatcat aggcgccgtt
    20161 tttcttgccc aaaaaatgaa aaaagtactg cttggacttg aaccacaaga aatagcagaa
    20221 cagttaagtg aaaaaacgat tatcgaaaat gaagtagatg aaggaatctt agccatctct
    20281 tctgataaaa ctatcctctt gatcaataca cctgcaaaaa acattttcta ccaaacagag
    20341 cagctattgg atttcgaagt aggaaaaaaa tcagtgataa tctatatgac gttttgttta
    20401 aagagacgat tgagcatcaa acgaccgttc ggactcaatc catgtattta aatgggatag
    20461 aaactatttc tagcgttaca cctcttttta gcaatgaccg ttttttagga gcagtcgcaa
    20521 ctttacgaaa tcgatcagaa gtaatgaaat taatggagga gcttgggggg acgaaacaat
    20581 atataaactc catgagagct caaacccatg aattcatgaa taaacttcat gtcatttctg
    20641 gtttgattga tttgaagaag tatgaagaag tcacccattt tatccagcag ctaaatcaaa
    20701 attataaaaa agagattggc caagtctcaa gtctgatcaa agtacctgca attgccggtt
    20761 ttttattagg caaaataaac gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg
    20821 attcttttat ccctaaaatg gagatggatg actccatcca ttcacttttg caagtgttag
    20881 ggaacttact tgataatgca aaagaagccg tgctacaaaa acatgaaaga aacggacagg
    20941 tccggattca tttaaactat gacacagaag ctgaagtatt catcgtgacc gtctgcgatg
    21001 acggtgtcgg gatatcagaa gaaatctcag aaaaaatatt tacgctaggt ttttcgacga
    21061 agggaatcaa tcgtgggttt ggattgaatc tcatccattc aatcgtcaaa cgccacggtg
    21121 gatttttaga attttccaaa caaacaaatg gcggtacaat cgtctatcta gaattaccaa
    21181 aaaaggaaga tcaaaatgat gacaaatgtc ctgataatcg aagatgatcc aatggttgca
    21241 gaattgaatc gtacttttgt ggaaagcatc cctaatatga acgtaatcga aagtttcaga
    21301 aatgtagaag aaggaaaaaa atatttcaga caaaaaaacg cagatctgct gctgcttgat
    21361 gtatacatgc ctggcgaaag tggtatccat tttttatctt ggcttagaca gcaaggaaat
    21421 gatatcccag tgattttgat cactgctgca gatgatatcg aaaccataaa gaatgtgatc
    21481 cgctacggtg ccttagatta tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg
    21541 atcgaaaagt atcttgcttt ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa
    21601 ttgatcgatc aagtattcaa ccacaccact cccattcacg aaataccatc attcatcgat
    21661 ttgcctaaag gtctgtcaaa actgacttta aaaaaaataa gtgcatttat tctaaaagaa
    21721 gatactattt tttcaacaaa agaactggct caaaaaagct cactgtcacg tatatccgtc
    21781 aagaaatatt tgaatttttt agtagaacaa gatatattga acgaaagaat caaatatttg
    21841 gaaatcggcc ggccgctaac ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca
    21901 gattactgtt gagtcatcga attatcccat acaaaatagt ccaagtatac agaagtaaca
    21961 actgtttact tggactattt ttaaacattt tattgacgaa tatccatgtt ttcttatctt
    22021 aattgacggg ctttaggaag agatacctca tttttcgttt ttccagtttc tattatttca
    22081 cgaaagttct cgaaacttgt cgaaatcata tttgataatg cctcatctgt atttgaacct
    22141 acatgcgggg ttacaagaac tctagggtac atttctacta attgctggat agcagggtct
    22201 ggaatcgtct cccatggctt gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca
    22261 tcggttgcaa aaccagctaa atgattgctt ttcaaagctc ttaaaatagc ttcattatct
    22321 tgaagctctc cacgggctgt attaatcaag atgctacctt ttttcatttg agctaaaaat
    22381 ccttcattga tcatttgatc gttttcacct gggatatgag gtacatgaat actgacaata
    22441 tcgcttttgg ctaataactc ttcaagcgag tggaacgtca atactttttt agcagcttct
    22501 gtctgatgaa tatcgtagcc gatcactttg gctcctagtc ccttaaacaa aacagcttcc
    22561 gtcaacccta tcctgcctgt tcctatgatc cctactgtgc aatttctgat ttcacgacta
    22621 aacatttgct catccactcg aaaatctttg aaagatgttc ggatcgtcgt atacgcagta
    22681 tttctcaaaa gcatcatggc aaaagtcaaa gataactctg ctatcgcatt tggcgaatac
    22741 gatggtacac gggctactgt cataccaaaa tcagctgccg cttgtagatc gatatgattg
    22801 actcccacag tccgtgtaaa aacataacga atcccatatt cctgcatttt tgcaagattg
    22861 gtctcatctg ctatacaatt tcccctcaac aatacagcat catggtcctt tgctgacacg
    22921 atattgtcat gtgtcaacag ttcttcataa agactgagat cataatggta aatattcaat
    22981 ttttcaaaaa aatctatctc gttcggacga acaccgtaac atgcgattct catactttct
    23041 tcctccattt cttatgaaaa taaacctatg ccatgaatca tttgtaatac aacgatccat
    23101 acaggtgtca gtaccacagc aaaaatagtt gacagtaagg atgcattgga ggctaaaagt
    23161 gcctccctat caaaactgat agcataggct gctgccactg ttgcagctgg tgtagccatc
    23221 atgataacga ttgttgccag tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa
    23281 actgctagaa tcacgatatt caatgcaggt acaaatacca ctttgactag actgtaatac
    23341 catgaagttt tttcagatgc agcttttttg aagcttactt cacctaatgt tgcgccgatt
    23401 gctagccacg ccaatggtga acttaaacca gctaaataat ccatcggctg aaatagccac
    23461 ggtgcagtct ggtcaattcg caaaaacgct acttggctta tctctccttc tgcacctgta
    23521 actgacactt gtggcagata cccttggaac agccaaatga aaaggccggc aaaagtagcg
    23581 ataatgattg gattgacgaa catcgctttg atatttttag cttccatctt caagccactc
    23641 atttttatgt atccataaga atacaaaaag attcggtagc caatattgaa aattgaggaa
    23701 tacatcacgc cgacaggacc ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta
    23761 gaaccaaaga tcgtcaggac acgcaacgtt gtttgcttat cccctttata gcttaagaaa
    23821 aaaggttttg aaactagaat aagaataata taaataacta atccccaaat caagacattg
    23881 attccttgca tcagtaattc tgtattgata tcttgcatga aagctcgaaa tgccagtgct
    23941 ggcaaagcca ctgttaagac gactttcgat agaatttttc caacagattc agaaaaaatc
    24001 cccttttttc tacaaacaaa ccctaataat ataataaaaa ctgttgaagt gattgcactg
    24061 acaatcgaca tatctgtcaa agtcgatcga aaaatgtcaa ataagttcat ctttctcctc
    24121 ctaattatgt tcatttattc acaataaaaa aagcttttat tttgctaata cgtattaaca
    24181 tttttaattt tatctttaca ttgttactta ttgaacaata ggaaattaaa gaaacttttg
    24241 aaacttatgt aaagtgattt ttactagaac acaaaaaaaa ccttaaaacc aaacttaaga
    24301 agtttagttt taaggttaac ttaaattata ttatgcgtgg aaacgttcca cgccatctag
    24361 ataagttgct tccaatgtca tatcagggtt caatacgata aagtctgcat cgcgtccttg
    24421 agcgattttt ccacatgtat catcgatttt acagctgatt gctggaatca gactagccat
    24481 catgactgct tgttctggtg ttgctaatcc ccagtctaca acatttttaa ttgcttcttt
    24541 caatttcaat atacttccag ctaagttgcc agattctaga cgtgctgttc cttcttttac
    24601 tactactgga aattcgccta aattgtaatt tccatcaggc atcccaccgg ccatcataca
    24661 gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc attaggattc ccgcagcatt
    24721 tggatgtaca tgatggccat cacaaatcaa ttcagaaaag acatgttcta aagataataa
    24781 cgcacccacc atacctggtt cgcgatgatt caatccacgc ataccattga aagcatggac
    24841 aaatacactt gcgcctgctt caacagcttt ttgcgcttct tctagtgtag cattactgtg
    24901 tcctagagaa acaactactc cttcttcagt cacttgtttg acaaactctt ctacaccttg
    24961 acgttccggt gctaaagcaa tttttttgat taaaccacct gaagcttctt gccattcatg
    25021 gaaagtatcc aaatcaggat ccccgaaata acttgggttt tgtgcccctt tgtgttcttc
    25081 tgtgaagaaa ggaccttcaa aatagattcc tttgatttta gctcctggtg cttcctgata
    25141 cattttgccg attgtctcag ctacatcttt taaacgttct ttgcttgacg tcaatgttgt
    25201 tggtaaaaat gaagtcactc cacaagataa cagaccttca gacatcactt tgatgccttc
    25261 tgcatcatta tccattacat catgattcat atagccgtga atatgtgtat ctaccaaacc
    25321 aggagcaatc catttgccgc tttggtcaat gatgttgact tctccctcag gtagttcttt
    25381 ggtgtattca ccaaaaagtc catcggtaat ttcaagatag cctgcatgtt tgacacctga
    25441 cgctaaaaag aatttgtcag catagataaa agttctcatt ctattctctc cctttgtttt
    25501 tctacctcta aagtactctt atttagagaa gaagtcaaga atggtctaca ccatttacaa
    25561 attttttgaa atttgtttca cagcttgtgc taagccttca tagaatgctg ctctttctgg
    25621 ttcttcttta cgcttgtata ttaagctttt aagcacaaat aaatcattga ggtaatagta
    25681 gctattcttc tcttgagccc aatagattcc ttgattcacc tgttctaacg cttcgtcgat
    25741 ttcattttga tggaataaga aatttccata attataaaaa atttgcgaaa attcactttt
    25801 tgatcgttca tcagctgatt gatgcagcag ttggatgctt cgactcaaat agtagcgtgc
    25861 ttcagccgtt tttcctgcca caccatatat ttttccgata caactaatca gttgtatttc
    25921 tgtgtcagaa acatggattc tttctttttg gctagagtag cttaatgctt ctttcaaata
    25981 ataaagggac tgagagatat tttgacgcaa cgtgaattcg caaacaccga gataataata
    26041 atagcgttga aagtcagttg cttgatacag attttcagaa atatcagaag attttagtac
    26101 ttgttctaac ttccgataat cacgtttttg gaaacaacta tccaacagtt caaataattt
    26161 atcgtttgtc cgagtagttt ttcgagagag attcatcaca tgatcaatcg ttacccctag
    26221 ccgttgacaa atctgcatca tcaccaatac attcggcagt tcctcattat tttcgatccg
    26281 acttaataca ctttgtgaac aaatatcatg cgaaagcatt ttttgcgtca ttttttgttc
    26341 ttttcgaatc tctttaatga tcgttccaaa ggactcgatt aacgtttcca taagtatcct
    26401 ccccaaaata tgcgtattcg cattctattc gtattttact ttaggaagtc gcaaacttca
    26461 agaaaagact tgcaactaaa taatatactt tagttattta agaaaactcc actagaaaga
    26521 gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa tttggttttt aaaatcattt
    26581 ttggcgatag gttgctagga attctctcat tttcttagct gtttgtttca aatcatccat
    26641 tttaggaaga taaacgattc gaaaatgatc tggctggttc caattaaatc caccaccatg
    26701 tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg aatttttcat catccaaaat
    26761 attgaatcgc tttgtatcaa tttttgggaa tatataaaaa gcagcttttg gttttacggc
    26821 tgaaagacca ggaatatcat tgattgcatt atagatatat tctctttgtt catagatcct
    26881 tccgcctggc aggagcagct catcgacact ttgataaccg cctagcgcgg tttggataat
    26941 ttgctgagac aatacattgg aacacaaacg catggaggac aacatattca gcccttctat
    27001 atagcctttg acgtgtgact tatctccact taggaccatc cagcctactc gaaatcctgc
    27061 tacacgatga gatttggata gaccgttcag cgtaacaacg aacaaatcag gcgctaatgt
    27121 agcaatcgga atatgttcta gaccatccat cactaagcga tcatagattt cgtcggaata
    27181 aataatcagg ttattttgtc gagctacttc aacgatttct tctaataatt cttttggata
    27241 aagtgcccca gtaggattat taggattaat gataacaata gcttttgtcc gactcgttat
    27301 ttttgatttg atatcatcaa tatcaggata ccattctgcc tcttcatcgc aaatataatg
    27361 aacaggtgtg cctccagcta atgcaataga agccgtccac aacggatagt ccggcattgg
    27421 aaccagtact tcatctccat tgtccagcaa cccctgcata cacatcgtga tcaattcgct
    27481 cactccgttg ccggtgtaga tatcattgat cgtcacattc ggaaaatgtt tcagttggca
    27541 atattgctca attgcttttc tagcagaaaa gatccccttg gaatctgagt agccttctga
    27601 gtcacgggca ttcatgatca aatcacgaat gacttcgttt ggtgcttcaa atccaaaagg
    27661 tgcgggattt cccgtgttca attttaaaat actaatgccc tcttcttgca tccgctccgc
    27721 ttcttctaga acgggaccgc gaacatcgta actgacacct tctaatttgt tggatttttc
    27781 aaattctctc atcacttagc caatccctca tttcaaaaag ttagaacttt ccaaattttt
    27841 tgttatttac tatttcaata tcatactcca tcctagaaaa ttttgccact tatttaggaa
    27901 aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac ttaattgtca tgatagtcat
    27961 tccatagatt cgctgcccaa tccattgcaa ttccacaaat gattcctagc ataaaaccaa
    28021 gcaaaaaacg tccgaatgca agtccaatca atacacctaa gatcattccc agtcctgaaa
    28081 attctccgtc atatttctcc atcttaataa gtcctcccat ttttgagctt gacaactatg
    28141 taaaacctcg ctatcattag tgtgttatta ttacgcagat gtgttggaat tggcagacaa
    28201 gcatgattga gggtcatgtg agcgttagct cgtgtgggtt cgagtcccat catctgcact
    28261 acaactcgtg aagttaattt tgcttcacga gtttttttgt ttgaacgttt ctttatgcca
    28321 ttatatacat atcactgtat cctttacaaa aagaacgtac aatcatgtaa acagcctgag
    28381 gtgagtatat gaaggtcgga aacaagctac tcgtagaaac ttgcaagtgt taaaacaatt
    28441 aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc gagggatttt ttgtatatta
    28501 tctgtttatc accgttatta taaaggtaca aataccaagc acggttaatc caaattgcat
    28561 aaattcattt ttttgtacac tcatgctttt cattagctct ttaactgcca ttagtactaa
    28621 gatggcgata acaacataaa gtatatagta cgtccgcatt ccggaagccc atactgtggg
    28681 agaaaatccc ataattaacc ttgaacaaaa tcccattatc atcaaaataa ctaaaaaaat
    28741 agaagttttc ttattgtcaa aacttaaata aattccaaca attaaacata gtaataatgc
    28801 caagactact aaagtagcta tccaagtacc tggataaaaa agtgacagtt tcgttcctaa
    28861 ctctgtaaag agattattca ggttactact actccaaatc attgcccttt tatttccatt
    28921 gacgtaagta aagctttgac ccattgtatt accaaaataa ataatcaggt ttaagaaaaa
    28981 cggtaatgca gtcaatatac gcacataata gttttgacat ttcctatatg ttagaaggaa
    29041 aattaagaaa aatagcaata aaaataagat attcatgtct aaaaaaaacg gctttccaaa
    29101 tgatgaaata cctaaatcta acttattaag aaagttgaaa tttttatatt caggaaacca
    29161 tttattaatt tcatgtgcag cacgtaatgc attccctgga gttgtcaaag agaaaattaa
    29221 ttctgctaag cttattattg aaaatactga taacttataa ttatatcgtc cattgacaat
    29281 caaatataaa ctaactatac ttgttagaac aaaaaaacag gcgttgactt gttcttgatt
    29341 agttaaaaat atcaataaag gaatacttac ccaatatatt tttctattta tttcttctcc
    29401 tcttaagagt tgaaaaaacg gataaaaagt tagtaaacta aatgctactg gccactgata
    29461 gttcaaggtg gttgctctcc aaccggtttc ccccattact gtcaatggta ttaaacagta
    29521 tatagagcaa accaataata aatttttaac atttattttc tcaaaaacat
//
LOCUS       DACDNO010000035        29264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDNO010000035 DACDNO010000000
VERSION     DACDNO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.035"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..129)
                     /locus_tag="IWI22_001584"
     CDS             complement(<1..129)
                     /locus_tag="IWI22_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9168978.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTD"
     gene            complement(241..1272)
                     /locus_tag="IWI22_001585"
                     /pseudo
     CDS             complement(241..1272)
                     /locus_tag="IWI22_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1354..2043)
                     /locus_tag="IWI22_001586"
     CDS             complement(1354..2043)
                     /locus_tag="IWI22_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9168979.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(2149..2517)
                     /gene="rplL"
                     /locus_tag="IWI22_001587"
     CDS             complement(2149..2517)
                     /gene="rplL"
                     /locus_tag="IWI22_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9168980.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2570..3073)
                     /locus_tag="IWI22_001588"
     CDS             complement(2570..3073)
                     /locus_tag="IWI22_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9168981.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3399..4088)
                     /gene="rplA"
                     /locus_tag="IWI22_001589"
     CDS             complement(3399..4088)
                     /gene="rplA"
                     /locus_tag="IWI22_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9168982.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4192..4614)
                     /gene="rplK"
                     /locus_tag="IWI22_001590"
     CDS             complement(4192..4614)
                     /gene="rplK"
                     /locus_tag="IWI22_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9168983.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4957..5625
                     /gene="sdaAB"
                     /locus_tag="IWI22_001591"
     CDS             4957..5625
                     /gene="sdaAB"
                     /locus_tag="IWI22_001591"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9168984.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5638..6510
                     /gene="sdaAA"
                     /locus_tag="IWI22_001592"
     CDS             5638..6510
                     /gene="sdaAA"
                     /locus_tag="IWI22_001592"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9168985.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6601..7620)
                     /locus_tag="IWI22_001593"
     CDS             complement(6601..7620)
                     /locus_tag="IWI22_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9168986.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7793..8680)
                     /locus_tag="IWI22_001594"
     CDS             complement(7793..8680)
                     /locus_tag="IWI22_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9168987.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8757..9302)
                     /gene="nusG"
                     /locus_tag="IWI22_001595"
     CDS             complement(8757..9302)
                     /gene="nusG"
                     /locus_tag="IWI22_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9168988.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9403..9573)
                     /gene="secE"
                     /locus_tag="IWI22_001596"
     CDS             complement(9403..9573)
                     /gene="secE"
                     /locus_tag="IWI22_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9168989.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9597..9749)
                     /gene="rpmG"
                     /locus_tag="IWI22_001597"
     CDS             complement(9597..9749)
                     /gene="rpmG"
                     /locus_tag="IWI22_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9168990.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9911..10555
                     /locus_tag="IWI22_001598"
     CDS             9911..10555
                     /locus_tag="IWI22_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9168991.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10611..11114)
                     /locus_tag="IWI22_001599"
     CDS             complement(10611..11114)
                     /locus_tag="IWI22_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9168992.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(11127..11173)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11711..12676
                     /locus_tag="IWI22_001600"
     CDS             11711..12676
                     /locus_tag="IWI22_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9168993.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12775..13947)
                     /locus_tag="IWI22_001601"
     CDS             complement(12775..13947)
                     /locus_tag="IWI22_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9168994.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13934..15079)
                     /locus_tag="IWI22_001602"
     CDS             complement(13934..15079)
                     /locus_tag="IWI22_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9168995.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(15093..16025)
                     /locus_tag="IWI22_001603"
     CDS             complement(15093..16025)
                     /locus_tag="IWI22_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9168996.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            16142..17221
                     /locus_tag="IWI22_001604"
     CDS             16142..17221
                     /locus_tag="IWI22_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9168997.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17218..17847
                     /locus_tag="IWI22_001605"
     CDS             17218..17847
                     /locus_tag="IWI22_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9168998.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17957..18184)
                     /locus_tag="IWI22_001606"
     CDS             complement(17957..18184)
                     /locus_tag="IWI22_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9168999.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18287..20875)
                     /locus_tag="IWI22_001607"
     CDS             complement(18287..20875)
                     /locus_tag="IWI22_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9169000.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20895..21554)
                     /locus_tag="IWI22_001608"
     CDS             complement(20895..21554)
                     /locus_tag="IWI22_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9169001.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21603..22106)
                     /gene="trmL"
                     /locus_tag="IWI22_001609"
     CDS             complement(21603..22106)
                     /gene="trmL"
                     /locus_tag="IWI22_001609"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9169002.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22327..23193)
                     /locus_tag="IWI22_001610"
     CDS             complement(22327..23193)
                     /locus_tag="IWI22_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9169003.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23302..23943
                     /locus_tag="IWI22_001611"
     CDS             23302..23943
                     /locus_tag="IWI22_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9169004.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(24005..25366)
                     /gene="mgtE"
                     /locus_tag="IWI22_001612"
     CDS             complement(24005..25366)
                     /gene="mgtE"
                     /locus_tag="IWI22_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9169005.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25384..26283)
                     /locus_tag="IWI22_001613"
     CDS             complement(25384..26283)
                     /locus_tag="IWI22_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9169006.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26285..27082)
                     /locus_tag="IWI22_001614"
     CDS             complement(26285..27082)
                     /locus_tag="IWI22_001614"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9169007.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(27060..27764)
                     /locus_tag="IWI22_001615"
     CDS             complement(27060..27764)
                     /locus_tag="IWI22_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9169008.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27895..28470
                     /locus_tag="IWI22_001616"
     CDS             27895..28470
                     /locus_tag="IWI22_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9169009.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28588..>29264
                     /locus_tag="IWI22_001617"
     CDS             28588..>29264
                     /locus_tag="IWI22_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9169010.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8612 a         6181 c         5261 g         9210 t
ORIGIN      
        1 atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata tgttagaaac
       61 agttctcata atttccttga atgttataaa agtatctgta taatgttcag tatattttga
      121 tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg cgtttttttg
      181 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgggt
      241 aaatacagta ttatttcggt tgcttgctgc ttcacctagc ccttgtactc gtgtttgatt
      301 ggcgacttga ccaaattgat tcccgacgtc taataaaata atagctaaaa gcaaaatggc
      361 caaatggctt ccgccaacca ataaagcaat aaagcttatt gtttgcataa caagtcccat
      421 atacacgatt tttctttcag aataagaatc tgccagtcgt cccacgatag gtgcagcaac
      481 tgctccagac aaaccaaaaa tcgctaatat ccctgcttcg aatgttcccc aattgtaggc
      541 gggactgctg atataaaaaa tcaacgtaga ccaaaagatc gaaaatgttc caaacatgaa
      601 aaatccactg actgccgctt ctcttaatag acgttgagaa gcaactaaat gcggcaaact
      661 tttaagtgaa tcaaggtatg aaacagctgt actactttgc tggacagaac gcggcatttt
      721 ccagtaaagc agaagtgtaa gaattccgac tgcacataaa gcaaataaat agatcatccg
      781 ccaagaaaaa agactggcaa caataccaga taccgtccga gaaagcaaaa tccccacgag
      841 taaaccgcta agcaatgtac ccatgacctt ccctcttgct tttggtcccg ctagtaccgc
      901 accgtaagga atgatgattt gcggcacgat cgaaagtaat cctattaaaa aagaagcgca
      961 tgcaaataac gtaaacgaag gcgcaaaaaa agcggccaac agagagacgg cagataacgc
     1021 taccattcga atgatcaatt ttggacgatc caccacgtcc cctaaaggaa ctaaaaacag
     1081 taaccctaac gcatagccta attgggtcaa catcgtcaat atcccgactg cacttgtgct
     1141 aacagataaa ctagctgcta cttttgttcc aatcggctgg atgtaataca tattggcaac
     1201 aacaacgcca caagtgattg ataatagtaa aataagcgaa gttgttaaag gtgttttttc
     1261 attactcttc attgatttcc cccatttcct atcttagttt acaacttatg gaaaagattg
     1321 gaaatagaaa aatacaacta ttcgccacac ttattatttg tgcttttgtt ttccttgttt
     1381 tttcttgcga tctttataaa tgaagacaac taaccctact agcacagtaa cacttgcaac
     1441 caacgtgact gcagttttgt tatatccgtg aaacagagct tctccaccac acgcatacaa
     1501 cacagacgaa gggatgactc caatcacgat cgacaatatg atttgctgta gaggcaattt
     1561 catcgcatct gcagcaaaat tgaccataga agatggaata accggtatca tatagcctaa
     1621 cattacacct atttttggat gtttcatttg tcggattgct tgcacccaat gattcgtttc
     1681 ggttttctta tctaaaaatt ttaagcgatg cataaggaaa attgcagtga tgttaccaag
     1741 agcattaccc aaaatgttga tgatcgcacc tgtcagtggt ccaaagctca aaccagcaat
     1801 gactccaact actgaagtag gaatacctgg aacagcacac atcaccgctg tcaatacgat
     1861 caagagtatc gatgcaaaag tcccatgcga acgaatatgt gcgagtagct ctgccttact
     1921 cgctctcgga tttaaaaata attggatgtc tgtctgatat tctaaataca accgatagcc
     1981 cactaccagt atcagaatga ttcccaacga gatcaacatc aatcggatta ttttcttttt
     2041 catatttcct cctgaaaaaa atctatattc attaaataat agtttaacat aaaaacaaaa
     2101 aagcaccgaa atccaacata ggatctcagt gcttttaaat agtaaaaatt atttaacagt
     2161 tacagatgcg ccaacttctt caagtttagc tttcaattct tcagcttctt ctttagatac
     2221 gccttcttta actggtgcag gagcgccatc aactacagct ttagcttctt tcaagcctaa
     2281 accagttgct tcacgaactg ctttgataac tttaactttt tggtctccag ctgcagttaa
     2341 ttctacagtg aattctgttt gttcttcagc agcagcagcg ccaccagcag cagctacagc
     2401 tacaggagca gcagcagata cgccaaattc ttcttcaata gctttaacta ggtcgttaag
     2461 ttctaggata gttgcttctt ttaactcagc aacgatgttt tcaatgttca atgccattat
     2521 aatttcctcc gtttaattgt tcttttttat ttaatgcttt atgcggcaat taagctgcat
     2581 ctccatcttc tttgtctgcc actgctttga cagcgtaagc cacgttgcgg actggcgctt
     2641 gtagtacaga tagcaacata gatagaagtc cttcgcgatt tggcaatttt gccaatgctg
     2701 tgatttcttc caatgaagag actttacctt cgatgatacc gcctttgatt tctaatgctt
     2761 ttgcttcttt agcaaattcg tccatgattt tcgctggtgc gactacatct tcgttactga
     2821 atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc ttcaaggcca gcttttttag
     2881 ctgcacgtga aagaatagaa tttttgataa ctttcatttc tacgttagca tcacgaagtt
     2941 gtttacgtaa acgtgttact tcatcaactg ttaatccgcg gtagtcaacg actactactg
     3001 atgcagctgc tgtaaatttt tcgcttactt catcaacgat tgctgctttt tttgcgataa
     3061 ttgcttcact catttttgag tttcacctcc tgaattttga tggaagagtt ttttgcactg
     3121 aaaaggcaag tcacttttat agttactttg ctcaaccagt attgttcatc atctcttcac
     3181 ttcgttcata gtgattcaca ataaaaaact ctatgccacc gcagacatag agggactatt
     3241 gaatatattc aataaacgtc ctcggtagga aattaaggct tgatgccacc tactgtcttc
     3301 ggtacaagtt aattattaac ctcaactagc ttagcatgct aaactagttg agtcaagaat
     3361 tatttttcag ttggatcaat caacattcta atatttaatt agaaagaagc ttggtcaaca
     3421 tggataccag gtccaaatgt tgttgtgatc gttacatttt tcatgtattg acctttagct
     3481 gctgatggtt tagcttttag caatacatca tggattgctg caaagttttc aactaatttt
     3541 tcatcgtcaa atgaaacttt tccgattggt acatggatat ttccagcttt gtccacacgg
     3601 taagttactt taccagcttt cacttcgttt actgcttttg ttacgtccat tgtaactgta
     3661 ccagttttag ggtttggcat taagcctttt ggtcctaata ctcgtcctag acgacctaca
     3721 gtagccatca tatcaggtgt tgcaacaact acatcaaagt caaaccatcc gttttggatt
     3781 ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc ttcttttgct
     3841 ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac cagttccgtt tggtaataca
     3901 acagcaccac ggatttgttg gtctgctttt ttagggtcta cgttcaaacg gtatgcaact
     3961 tcaactgttg catcaaattt tgcaatgttt gtttcttttg ctaaagctac tgcttcggca
     4021 acaggataaa ctttacttga atcaactttt tttaatgcat cttgcatttt tttgctcttt
     4081 ttagccattt tggttcctcc ttgattgtgg ttataacggt tgtacctccc acgtgttcta
     4141 tatctttcaa tatagagcca actgagaagc tgctgtctta gggtgtatag attattctac
     4201 agtgatcccc atgcttcgtg cagttccttc aaccatgcgc attgctgctt caacgttagc
     4261 tgcgtttagg tcttccattt ttaattcagc gatttctctt acttgatcgc tagaaacttt
     4321 cgcaacttta tttttgtttg gttcgcctga acctttttcg attttagctg cttttttcaa
     4381 tagaacagca gctggcggtg tttttgtaac gaatgtgaat gaacggtctt cgtatacaga
     4441 aataacaacc ggaatgatca aacctgcttg atcagctgta cgagcgttga attcttttgt
     4501 gaatcccatg atattgatac ccgcttgacc tagtgcagga cctactggtg gagctggagt
     4561 tgctttacct gcaggaattt gcaatttaac gattttttct actttctttg ccacgagaca
     4621 tacctcctta agtccgtgat gtggttaatt ggagatgcat atttctcctc ccacgtttta
     4681 tctgatctga aaaaatccag acaaataatg tgcaaaacaa tgcacactgg tacagtatag
     4741 caaatttatt tgttttttca agattttttt ctgctatttt tacattttgc ttgaataaac
     4801 gctaaattta ttctaaaaaa acaacaattt ttcgttctca tttcttcttt tatgttacac
     4861 ttgcgctagc atggttcaac agatgaaagc aaacaataac acggtttttt agacccttgt
     4921 acaaagattc ctacataaca aaatgaggtg gagaaaatga aagatttaag gtatagaagt
     4981 gtctttgata ttattggacc tgtgatgatt ggtccaagta gctctcacac tgccggagct
     5041 gctagaatcg ggaaaatcat acgtagtata tttggcgaac agcctgattc tgtagatatt
     5101 tatctatatg aatcatttgc aaaaacctat cgggggcacg gaacagatat tgcattagtt
     5161 ggcggcctgt tagggatgga acccgatgat gaacgtttag ctgattcttt gaaattggca
     5221 tatgaagccg gtatggaagt cttgtttgtt cctaagagtg aaaaagccga acatccaaac
     5281 tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg catttcgatc
     5341 ggcggtggca atatccagat ttctgaattg aatggattca aaatttcttt aagcatgggt
     5401 actcctacct tgatcgttgt ccaccaagat gttccaggta tgattgctaa agtgactagt
     5461 atactttctg agaccaatac gaatatcggc actatgacag tgacacggga atcaaaaggt
     5521 gaaaatgcca ttatgataat tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga
     5581 ctcaaacagc tgccaaatat cgacagcgta aattatttcg attaaggagc taaaaaaatg
     5641 tttttatcga ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt agcagaattg
     5701 atgattgcag ttgaaatgga aatgagtggc cgttcccgta gtcacatcat cgagatcatg
     5761 gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg gaaaagaagg tgttacctcc
     5821 gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg attatttaaa tgaggcagac
     5881 tttttaagtg gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc
     5941 aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct
     6001 ggtgtgctga tggctgtccg agatcggctt gatttgacac atgaacagca attggaattc
     6061 ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca acgcttctat cagtggtgca
     6121 gaagggggat gtcaagcaga agttggttct gcaagtgcaa tggcagcagc agctttggtt
     6181 tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag tagccattac attgaaaaat
     6241 atgatgggac tgatttgcga tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt
     6301 aatgctttag gctcttctca agcttttatc tctgctgata tggcattagc gggtatcgaa
     6361 agtgtcattc cgccggatga agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg
     6421 tcgattttca aagaaacagc agaaggtggc ttagctgtta cgccaactgc taaacgactt
     6481 gcggaagaga ttttaaataa gagggcgtga taaagctcct taatttcgag tattaagaga
     6541 ttagttatct tcataacaaa aaacgaagag aggactaatt gcccttctct tcgtttttta
     6601 ttaatccatc aatttcgtat tttttacttc atcgatcgtt tttgttcctg caagttgcat
     6661 cgtgatcgac aattctttgt tcaggtgttc aaaaacagat gttactcctt ctgccccacc
     6721 tagattcaac ccataaatca ctggtcggcc aatcgcaact aagtcagcac cacttgccag
     6781 agctttgaag acatgctctc ctcgacggac accagagtcg aaaatcactg gtacacgttt
     6841 gttgactact tcggcaattt ttggcaatac ttcaaaggac gcaggacctc catctaattg
     6901 acgtccacca tgattggata cccagatacc atctgcccct gcagagatag cgattactgc
     6961 atcttctggc gattgtattc cttttacgat aactggaaga tgggtgattt ctttgattgt
     7021 tttgatgtca tctggtgtca agccttgttt tgcagctgca taaatttctg cgattccttt
     7081 tccttcgcca ttaccacttg cactttgttc gctatacgca gctaagttag gcattggtaa
     7141 tggaaattgg aattgattga tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc
     7201 cgttaaaatg atagctttag cccctgcttc tactgctttg ttcaaaatga actcattgaa
     7261 tctgtcgtct ttactcatat ataattggaa aaactgcggt gcgcctggcg ctgcatcggc
     7321 tgcgtctttg attgtcgtat ttgcataagt gctgatggaa aagattgatc cagcagcagc
     7381 tactccttta gctgtatcag cttccccttt ttcatgtgcc aatccttggg ccgctgatgg
     7441 cgcttggata atcggtgtat ctaattcaat tccgaaaaca gatgtatgca aatcggctga
     7501 gtcgattccg cgtaatatcc gtggcatgat tttctttgta ttaaaagaag tcgtattttc
     7561 tttcatcgtc cactcatctt cagaaccacc gcgaatatag ccaaaagctc ctgcttccat
     7621 gcgctctttt acacgacctt ctagttaggc aatgtttaca atatcgattg gatgttcttc
     7681 attacttgta tgataagtca tagtagatcc ttctttcatc ttcttgatta catataaatc
     7741 atactaaact tacaaaaagt aagcaaacat ttactctcag atacttttat cgctattctt
     7801 tccaaataaa tcgacttaca agctgatcta cttcttggtt ttcatgaagc atactatgag
     7861 aggcattttc acctgtcact actttttcct ctatttcatt tccgcgttgc ttcaacaacg
     7921 catagaccgc taatgcactg tttaacggta ctgttccatc actcaagtct gtttcatcta
     7981 gttgaccagc aatcaagaaa aatcgagtgg ttataggaat attaccgatc atttgctggt
     8041 aatcttgata gcggttagaa acgacagcag gcccctttgc taactcatct gtcagagact
     8101 gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat gtctgaactt
     8161 gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta actcctccca
     8221 tggaatgccc cactatgttg actttatcta tgccgtagtt ttctttcaga tacaaaagac
     8281 aagttttgat ccattctgct tgattccact cgttattttt attatcttca aacaatactt
     8341 ggaccatcgg attgtctgct tttccagaca aatctcctgt tgcttgtatt tgtccatctg
     8401 ttcctactgt taaaaccaat tcttttactg cttgctgatt tttttccaaa cgccgcaaca
     8461 tccctttaaa ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg
     8521 ttttttctat cactttactg gattctgccg ttttttcttt atcagaaact tcagtagtat
     8581 ttatcttcgt cgtctggcaa ccacttaacg caaacaaact gataaggact aaagctgctg
     8641 cagttgcacg tttgcataaa tttttgattg gttctgtcat acacctcttc ctttccatat
     8701 agaaaaaaga aagacttgaa aggccaatca tatgatcgac atttcaagtc tctttattaa
     8761 tcgattttat ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg
     8821 atgttgactt tcaatttttc tttttctccg tcaacttctg ttacttgtcc ttctaatcca
     8881 gcaaatgcgc cttcgatgat tttcacgact tcgccaactt ctacttctag atcattttgt
     8941 cgcaggctca taccgataga acgcaagata tgattgactt cttctggaag taatggtgct
     9001 ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca caccaggtgt gttgcggaca
     9061 acataccatg aagcgtctgt catgatcatt tcaacaagta cgtatcctgg gaatgtttta
     9121 tgaacgattt ctttttcttt cccatttttg acttcagttt ctttttcttc aggaacaacg
     9181 acacggaaaa taaagtcggc cattttcata ctttgcgcac gagattcgat atttgctttt
     9241 actttgtttt catatcctga atatgtatgc aagacatacc agtttttttc aaaagattcc
     9301 attgaaggct cctcatttca ttgtaaaata aaaaaacctc agtacacccg aagtttttct
     9361 ttcgcttcat tatagcacta aaagtacgtt gtggctagcg agttatttta aaatccagcc
     9421 aaataagctt tggatggctg tatccattac gtaaaacatc aatccgaaaa taatagaagt
     9481 ttcgatgact actaacgtat ctttacggag ttgttttctg cttggccaag ttacttgttt
     9541 gatttcatct tttacgcttt ttagaaattt catgagacaa cctcccttgc tactgtttat
     9601 ttcgtttctt tatgtaatgt atattgatta caatatttac aaaacttatt gatttccaaa
     9661 cgttcgccgc tttttccttc actgactgat tttgtgtagt tgcgagaccc acaaacggaa
     9721 caagcgagtg ctgctttttt ctttgccatt tacttctgcc cccttgctga gaatcagact
     9781 tatacttcat aacagtatca cgtaactttt tacttgtcaa tttgtgaaat aaactatcta
     9841 ttgttttgga taaaattatg atatgattaa tttgaaatta agctaaaatg agacaattgg
     9901 aggtatagcg atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac
     9961 agagactagt acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt
    10021 gccgatctta gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta
    10081 tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc
    10141 tacaaagttc atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct
    10201 tccttatatt gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac
    10261 gttgcttaat atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat
    10321 tgcttccaca ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag
    10381 cagcattgcc agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt
    10441 gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga
    10501 gaaaaagaat tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa
    10561 aagcgaaaaa agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc
    10621 ttttcaaaat caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg
    10681 ataaactcac ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga
    10741 actaacatat aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa
    10801 cgcatcttta catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat
    10861 gtttggccgc caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg
    10921 atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa
    10981 ctttctgaaa tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac
    11041 gctagataaa gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt
    11101 ttcgtttttt gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat
    11161 gaaccgcggt tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg
    11221 tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg
    11281 ggatatggta taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta
    11341 ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt
    11401 gagcaacctc ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg
    11461 aaccttggaa cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt
    11521 ttttcggtct tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa
    11581 ttttgtaaca gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt
    11641 gctaattatt ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca
    11701 ggaggaaatc atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa
    11761 tggctataaa gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac
    11821 acttcattat agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga
    11881 aacattgatt ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat
    11941 catcgtagga ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt
    12001 agaattccca gttgcttata ttccagcagg ttctggaaat gattttgctc gaggggcagg
    12061 ccttccaaaa aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa
    12121 agtccacata atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa
    12181 tttcggtata ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa
    12241 gcgattgaac aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt
    12301 attcactcaa aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc
    12361 aaacgcattt ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt
    12421 tccaactgct gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt
    12481 ctttaaaatc ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata
    12541 tttccatcac tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca
    12601 agaagatgga gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca
    12661 gttgttttgg tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt
    12721 cggaaaaaag gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt
    12781 ctttgcccaa ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat
    12841 tgaaaacacc gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa
    12901 tgtttgattg taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt
    12961 tggtgtgaat gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac
    13021 cccaccgata aagcaactgc ctgtgataaa aatatctttg atcccaccgc tggtttctga
    13081 gttttttacc aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa
    13141 agtatttaaa atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac
    13201 cattagaagt aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac
    13261 tttgcgattc aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt
    13321 gcgattaaac gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata
    13381 agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc
    13441 acttagctca acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc
    13501 gattgtttta tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg
    13561 tgtaacaact gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcgccatctt
    13621 tcctacttcg acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt
    13681 aaaataaaga gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc
    13741 cttgtcattc aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa
    13801 ggcgattttc gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa
    13861 tgtggcaaca acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa
    13921 tgtccaaatc gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta
    13981 acgcaaaaag gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat
    14041 cagtgtagaa ataaagttta tacaaacttt cgctgatcaa gttgacgaga tttaattgac
    14101 caacaatcgg aatatgaaga tcaatccaat acttcacttg ttccgacccc atcataccag
    14161 caaaaaagct catcgcaatc gtcactgaaa tcaagatgct atccttttgc accagattca
    14221 tttttggtat caaattacca accaatgtac ctaacaaaat cgcacacaac gaccccagag
    14281 cggtaacaat catcaaccat tgccaacgtt caccaaagtc tacttgatag ataaaacgaa
    14341 agacagctaa cataatataa atctctgtca aaacaatcgt aaagcttgct gccaaattag
    14401 cgattgaaac taacgattgc tttctaggaa tcaaggacaa gcgaatacca tttgacgatt
    14461 gatttgcttg ctgatcctgt acattgcgga ccccccacat cgtcccgtag agaatcgcca
    14521 ttccaatcag agtgaagaaa taaaaacttt tgaaactcat attacgacct gcatgaactt
    14581 cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg cgcgagatca acgttctcca
    14641 cttctgccac tttactcgaa attataccaa tacgctgtaa aaattgatcc atgacgtttt
    14701 tcatcaacgt ctgactcatt cctgcttgac ccacatggag cgaaacagat gaaccatcaa
    14761 tgagataaaa accagccaaa tcatctgcat ttagcttttc attcgcttct acttccgata
    14821 gttcctgtac ttcaaataat ggtttatcat tgacttcgac ttgttctaag actgtggtaa
    14881 aattttccga aagcgctgtt tgatccgctt tgattacaat tccagtatta atcgtttcaa
    14941 gattgccacc ttgatcaatc tcgccaaagg ccgccataaa catcaatcct aaaagaattg
    15001 gaaaggccaa tgtccaaaac aacatggatt tatttcgcag cagtattttt gtgcgatatt
    15061 tataaaaatg ccaaaacatt atttcccctc ctctagtctc tcaaatcctt gccagtaatc
    15121 tctaaaaaga catcattcaa ggacgcttct ttggttgcta tttttccgta actgatcttt
    15181 tcttcttgta agattgtaag aatcggcaac aatactgtat ttagttcagt agttgttact
    15241 tggagctgac cttttttata agcgattcca tcagaagcta ttgttcgctg aatcgctgac
    15301 aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata
    15361 tcttttagag attctttggt tcctttagca atcacctgtc cttgatccat gatcacgatc
    15421 tggtcgcaaa gaatttctac ttcttccatg taatgtgttg tatagacaat ggttgctcct
    15481 tgtcgattca gctccttgat actttctaaa attttattcc gactttgggg gtctacagct
    15541 actgtcggct catctaaaaa aattaaccgc ggcttgtggg caatcccaca tgcgatgttc
    15601 aaccgacgtt tcaagccacc actaagttct cctggacgaa acgaaacata tttttctaat
    15661 cccactaatt gaatcacctc ttcaatcaag gctttccttt tctctttttc ggaaacatat
    15721 aaaccgcaaa aataatcgat attttcttgc acacttagtt cgtcaaagac cgcaacgtct
    15781 tgaggaacga ccccaatcat tgccttgaca gcataatttt caggagtcat tgttgtcccg
    15841 aacaatttaa tggttccttt gtcataattt aacaaagaaa gcatacagtt gatggcagtc
    15901 gatttcccac tgccgtttgg acctaacaga cctaagactt ctccttcttg tacttgcaga
    15961 tcaaaatgat tcaatgctac aaaatctcca tagcgtttga ctaatttatc aatttcgata
    16021 atcatcatga aaaactcctt ccaaatttct tgttgatctg actataataa agtgcaagaa
    16081 aagaaactag tgacgattgg ctaaaccaca catgacattt gtcacaatga aggagtctgc
    16141 tatgcataaa tctcggctac tcaacgaaac tagctttcta ctggttatct cattgctttt
    16201 gctggtaagt caaggattcg ttgctatgca agttatctat ttattgatcg ccgttttatt
    16261 cactggtgtc agttatttac tgcctacaaa gccaaattgg attttccaag ttattctatt
    16321 tcttttatgt gcctttttac ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat
    16381 cgcccctgaa gaaagccacc ttgattactt gtttttgtta ggttttttgt tgatttcgac
    16441 ggttggtaat catcccccca tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt
    16501 ttacttacga aaaaaagatc aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa
    16561 agatactcat tgggaaaaac aacaagagtt atcggaacaa aataaagcat tggccctgtc
    16621 acaagaaaca caattagcct tgcaaattgt ggaagaacgg aaccgaattg cccgagacat
    16681 ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta caaattggtg cgcttgaaat
    16741 gatcaatcaa gaaccaaaac taaaagaacc attagaaaat ttaagtgcca ccgttcacac
    16801 tgggatggat cggattcgtc aaagtgtcca tgacctccat gagacatcga tctcttttca
    16861 acactcctta gagtttctga tcgctgattt tccttcttct gttacgattg aaggaaactt
    16921 gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta atggtcatca aagaagcctt
    16981 aaccaacagt atcaaacata gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc
    17041 ggccttttat cgcttgcaaa tcacgaatga tggcaccagc ccacaaaaaa attctgctcc
    17101 cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa caaaagggac aagtccatat
    17161 tttccaagaa aaccagcaat ttcagttaac gatcatttta ccaagggagg gacaccaatg
    17221 ataacagtag tcattattga tgatgatcca tttgtgacga aatcgttaca aaccatttta
    17281 gaaagcacga aagagatccg tgtgttaggc attggtcatt gtgccaagga tgcgctagtc
    17341 ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca tccgcatgcc taaacaaacg
    17401 ggcatcgatg ctgccaaaga aattttggct aaatttccca aagccatcct tttgcttttg
    17461 acgacattca aagatgaaga atacatccgc gaagcatttt ccatcggtgt caaagggtat
    17521 ttaatcaaac agaacttgca agcgattatt ccttctgtca aatccgctta taatggacag
    17581 gccgtatttg gtaatgaaat catcgagact ttcactcaaa tgatgaagaa taaaccgacg
    17641 attgatagtc atgcttatgc ttcttttagt gaacgtgagc ttgcaatcat caaagaagta
    17701 gctgccggga aaaacaataa agaaattgca gatgccttgt acttgagtga cggcactgtt
    17761 cgcaattaca tcagccaatt actagaaaag ctagatttac gggatcgcac gcaattggct
    17821 atctattttt atcagcattt gcaataagtc ttatctaaca caaaaaaacc tgaatgatgc
    17881 tccttcgcat cattcaggtt tttttcacat cttctattca attgtcccgc aaaccatttc
    17941 ctcacgattg aaagaatcaa ggattcgcag ggctgccagg attcgttgga ttgttcggcg
    18001 tgttaggcgg aaaaataggc gccttgtcta acgttttttc tagttttgat tcttcacgat
    18061 cgtctcgttc tacatcagga ctttcattca atttgaattc tttttccatc tcatctaact
    18121 ttttaaagtc attgccagat tcttttgcta atttcgctaa tttatcttcg gtttctttag
    18181 ccataaagta acgcccccct tttgatttca gcttagcata tcacactgaa aaaacaaaag
    18241 aaaacgctaa ccaacgatca tttttagcta tttctcatct ttaaaatcaa gcattttttg
    18301 ccggtataaa atcgtaggga gaactgtttt ccataccaat catcggatct actaactggt
    18361 tatcgatcac ttctgctgta agaacgaaat ctttagaaac agcaggttct acctcttctt
    18421 tcttttcttc tccaacaaat gaagaaaaca gatctggttc cactgtttcc attacttggt
    18481 ttttcgtttc attcgtccca ctgtcatctt ctacaggtag cttttctttt acaacccgtg
    18541 gctcttttgt atagttccca tcttcaaatg gatcatttcc gaccaaagaa tcttcataag
    18601 ctgctagttt ggtccggaga gtgatattca ttttatcatt atccatgatc cttgatttca
    18661 acgtagtcat ccggcttgct gattcattcg ctacacaagt gacataggct tgctcttccc
    18721 ctaacaaaat caacagttcc ttactgcgag tcactgccgt gtagagcaaa ttacgctgaa
    18781 gcatccgctg atattgatgg accatgggca aaataaccat cttgaattca ctgccttgtg
    18841 atttatggat tgagcaacaa taagaaagag tgattttatt ccattcgttt cttttataag
    18901 tgacctcatt tgcatcaaac tggataacta attcatccac tttatcttca gaatcttttg
    18961 ccaagatgat acccgtgatt tctcccatat ctccattgaa tacattcaat tcgggagtat
    19021 tgactaattg gaggactttg tctcctatcc gataaacggt gtcgttccaa tgtacttcct
    19081 tttttccagg tttccctgga ttgaatatct cctgcatcat tttatttaac gcatcgatcc
    19141 ctgcaggtcc ccgatacatt ggtgctaata cttggatatc ttgtgcacgg aatcccttat
    19201 ttttcgcttt ttccacaatt ttagagatca agggttcaat ttgataagca tcacttcgga
    19261 aataggaacg atctttttga ttctttgtga aatcttttgg cagacgtcct tctttgatct
    19321 catgggcaag aggaataata ctagaaccat caccttgccg ataaatctga ttgagttcca
    19381 ttttgggaat ttgttcgatt tccaaaagat catggagtac ttgaccagga ccgacagagg
    19441 gaagctgatc tttgtctcct actaagataa cttgcatatt cgtcggaatc gctttgaata
    19501 atgtgttagc cagccatgta tcgaccatag acatctcatc aacaatcaaa agtccgcctt
    19561 ctaattcttt cgtactgact gaaggtgttt tttcccgtcc attcagcccc agaagacgat
    19621 ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg tttagctgct cggccagttg
    19681 gtgctgcaag tagaatagga aaaatttctt gggtatattt tgaaggatcc aattccagtc
    19741 cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac agtagttttc cccgtacctg
    19801 gtcctcctgt taaaataaat agaggggaac ggattgcttc agcgatagct tgttcttgcg
    19861 aaggtccata ctgtatgccg aatatttttt cgatacgccg aatattcttc tgcacttctt
    19921 tttcaggata atggatttct tttttacgac tgagaaggcg ctggatcgaa ttaccgatcc
    19981 cccattcaga aaagtacaaa ctgttttcgt aaagattagt ctcttcttgt tgtatctttc
    20041 cttcttcaac aagttggata attacagtag caacctgatc aggctggatt tcaattgggc
    20101 gacttgtttc taaaatacgg atcgtttgct gaagcaatgt ctccgccggt acgtatgtat
    20161 ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat agctgctcga attcttctcg
    20221 gtgaatctgc tgcaataccg atttgttcgg ctagattatc tgcacgtttg aacccgattc
    20281 cttctatgtc ttcgactagc tggtacggat tttcttcgat gatatccaaa gactcgttct
    20341 tataggcctg gtagatactg aaagctaact gactaccgaa cccgtaacga ttcagtccca
    20401 tgatcacttg atccatcccg tggtttagcc gaatcgtctc agcaagcatt tctctttttg
    20461 gcttcgtcaa accggagatt tgttttaata ccgaaggatc agcaatgatt ttttcgatag
    20521 catcttctcc cagcaattct acaatttttt ctgctgtttt cttcccgatt cctgggaatt
    20581 tttcgctgga aagatacgct acgatcccat ttgcagttgt tggcttttct tgctcatatc
    20641 tttcgagttt aagctgcctt ccgtatttag gatgatccac taattctcca taaaaacgat
    20701 aagtctcatt ttcttgaata tcgccgaaac ttccggtgac tacaatctct ttttcacggt
    20761 aatcagctgt ggtttcactg atgtgtacca aaagcacttt gtaaaaatta ctaggatttt
    20821 gaaaaaaaat cgcggcaact gtaccaacga aataaggcat ttcttctggc tgcatcggaa
    20881 gccgcctcct tcttctatag taaaggttct ggtgctgttt cagttcctaa aaaatctgtt
    20941 tggttccata ctttcagttc atatggcaat aatctgcaag gctcttcagt ctctaaaatc
    21001 gtcaaactac tattgaacaa tccacccatt tcacgcaact cacttagctc ttttcctgtc
    21061 atccattgaa tggctgcagt taaagaagct ccatgtccta cgaataaaag tgggccatct
    21121 ttatgtcggg caaccgcatc tgctacaaca gtttcgatac gtgtgattgc cgattcaatc
    21181 gtttctccgt gaaaaggaga aggatcatac agatctaaat gattacgcaa attcggcaga
    21241 ttatccggat atttctggta catttcatcg atcgactgac cttctaaagt acccagccct
    21301 agttctctta gcttatctgt ataaaaaatc tctaccggac gagcaagctc tttgttgata
    21361 ccttctgctg tatcacgtgc tcgctttgct gtactggaat aaattgcttt aaaaggaaca
    21421 tattttacct ttttcccaaa tgctttgatt tcttcgtaac tttgtggcaa aagcggagaa
    21481 tcgccattgc tcccttgaaa acgtctttcc tgattccatt gtgtcttccc atgtcgggta
    21541 aaatatagtt ccatacgcag caaactcctg tctttctttt attttactct ataataaact
    21601 gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt ctaaattagg gaatccttgc
    21661 tggcgaagtg cttcatacac tatcattgca gctgtattcg ataaattcaa tgagcggaca
    21721 tgctcatcat tcatagggat gcgtaaacac ttttcttcgt tttcacgcat aaatgtttct
    21781 ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct cttctccatc agcaaaattt
    21841 tcgtcactat atactttatg ggcaaatttt gagatcaaat gtagtttacg attacctaaa
    21901 aatgttaaaa aagcttctaa attactgtgg taagtaatat ttacatcatt ccaataatcc
    21961 aaccctgccc gttttagatg tttgtcatca gttgaaaaac ctagtggctc gattaaatga
    22021 agcggagtat tcgtggctgc acacgtacgg gcaatatttc cagtgtttgc agggattaat
    22081 ggttcaaata aaacgatatg attcatcagg atcattcctc tcatatttat gttcgtttct
    22141 attttctgac aatttgattt ttgcataaaa aaaaacgtat caactcggtg tcagtcactg
    22201 cgagtcgtta cgttagtgaa gctcttctca ctaactttta tcagaacagg aacctgataa
    22261 aaaccaatga aaaaacaact tgttgctaat ataacaccct caactttttt ttgcaacctc
    22321 tactcattaa aaactgctag aaaaacgttt tctcttagtt ccttttaata attctatgtc
    22381 aattgtaatc ttcttcaaag gattcttata taagtctgct ttgattcgct cttctacctg
    22441 ccattctaaa ggtgacatgg ccaaaagatc tttttgattt tcctctggta tctcaaattc
    22501 ataggttaca cgctgacgtt cagttaattt cattgcttca gcaaacttgg ctactacacg
    22561 ttcattggaa taactctgtt tttcaggttg attcggataa aaagcctgac gtagttcctt
    22621 taaataattt tcttgtggca ccaccttcaa cacttctcca tcttctgaga gtacacgttg
    22681 gaattcttgg taatgagaag gcgaaaagat atttaaaacc gcgttgaatg tttgatcagc
    22741 aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct tcggcagatt gttctgtagc
    22801 tgcgtagact ccttcttttg aaatatcaaa tccaacagca ttttgaatct ttttcaattt
    22861 tgaaagttca ttcaagaagc taccttctcc gcagccaaca tctaatagcg agacttcttc
    22921 aggtaaatgc tgcgcaatag tctcaataac gggctgatac atgccagatt ggatcattct
    22981 acgccttgga acgaacattc cttgatcgta ttctgtttta accgcatggg ataaaaaata
    23041 aagtgttcct tttttggaaa ggtcaaaacg gtgattattt ttgcatatca agccattatc
    23101 agactttttt acttcttctt ggcaaaccgg acagcggaac agctgatgat tttctgccaa
    23161 aaactggcgt cctcggtcaa ttttttttaa catagttccc cctcatttct gttttatctt
    23221 accgttagat gctcactttg gcaactgcta aaaatatgat acaatgccta gaagaaagtg
    23281 aaagataaag gagaagatta gatgatcaaa gaattttgcg ctgaaaacta cacatctatt
    23341 cctctagcaa ttgctagagg agccgatcgg atcgaattgt gtgacaactt agcggtcggt
    23401 ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa gttatgctgg ggaaaaagat
    23461 atccccgtaa tgacgattat tcgtcctaga gggggcgact tcgtctataa cgatatcgaa
    23521 ttgaaaatca tgcatactga tttgattgaa gcaaagaaat tagggacgga tggcgtagta
    23581 tttggttgtc ttactccttc aggctggcta gacgaagagg ccttagaact attgatcgaa
    23641 acagcagaag ggttgcagat caccttccat atggcttttg acagtattcc tgaagatcgg
    23701 caattcgaag caatcgattg gctggtagaa catggtgttg accgtatctt gactcatggc
    23761 ggtcctgctg acaaaactat tgtagaaaat ttcccacggc ttaaagaatt gattgcctat
    23821 acagatggcc gcttaatcat tttgcctggt ggtggtgtca ctgcagagaa tgcagcacat
    23881 gtagctaaag aattgaacgt aagcgaagta cacggaacca aaatcgtgga tcttcagtta
    23941 taatcttata aacaaaaaaa catttgcaac aggcataatg cttattgcaa atgtttttgt
    24001 ttttttacat aaaatagccc ataaataaag aagcaatatt gaaataaatc agtacactcg
    24061 tcaaatcgct caaagtcgtg ataaagggac cgcttgctac tgctggatca aatcctagac
    24121 gatccattag catcgggatt aaactacctg caagattcgc cactgtgatt gcaaatagca
    24181 tagccatccc tatgacaaac cccaagataa aattatgttt ccaaaaacct acgattagaa
    24241 aaatcgtgat ccccgtaaca gctcctgtga caacgccggt caagacttca gccaaaacaa
    24301 gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag tcgacgaaca gccacagcta
    24361 gcgactgggt tccagcatta ccggcagttc ctgtaatcag cgaaatgaag actgctaaaa
    24421 tactcgcttc acttaccaag tcttcatagt gactgattaa agaagcggtc gacataccta
    24481 aaaacaacag agtgatcagc caaggcaaac gtcgagacgc tgcctttact ggattttcat
    24541 taatttcttc cacattgacc ccggcaagtc ctgaataatc gctagcagct tcatcatcaa
    24601 taacgtcgat gatatcatcg actgtgacga ttcctaacaa atgatcatca taatcggtaa
    24661 caggaagagc taagaagtca taatctctaa atgtttgtgc cacatcttct tggtcgtcgc
    24721 ctacatgaac tgaaagcact cgttcgctca tcagatcaga aatcattgcg tcgtcatcat
    24781 tcacgattag gtcgcgcaga gaaataaccc ctactagctg accttcttga ttaacgacat
    24841 agatgtaata gattgtttca gctacatctg cttcgttctt cagcacgtac atcgcagagc
    24901 gaacagtttg attggctacg atcgaaacaa attcagttgt catgatcgcg ccagccgttt
    24961 catcctcgta atggagaagt tccttgattt cactggcatc gtctgccgac atcaaactta
    25021 aatatttagc aatttgcttt ttatccagcg tattaagcaa gtcaacagca ttatctgtat
    25081 acatctcaga aagcatatca gctgcatagc taggacgcat ttcatctagg tattctttca
    25141 tatgttcgtt gtcttcttca atcacatcga acatatccgc taattcttta ggcgacagat
    25201 aggaatagat cagctgcctg ttctcttctg taagagattg gtagaattgc ccctgctcat
    25261 agatatgcat ctctaaaaaa ctgtttctga atgcctgaat attattttcc cttaaatctt
    25321 gagtcaactg ggcaaatctt tcctctaatt cttgattttc atccaaacca atctcacctc
    25381 gccttattct tcaaaccgtc tcacaactgc ctccatgtct gcggctaaag gctgcttgaa
    25441 ctccagcaac tcttctgtaa atggatggta aaaacgcaga tcataacaat gaagggcttg
    25501 tctttttatt cccagatcca ttcgcccgcc atacatatca tcaccgagca gggcacagcc
    25561 aattgcggaa aaatgaactc ttatttgatg tgtccttcct gtatgcagct ggatatcaac
    25621 taacgcgatt tctgttagtc gcttctctat ccagtactca gtctgtgccg aacgtccttc
    25681 atcagagacc attcgcttca atagcgaaga ataatctcgg gcgatcggca aatcgatatg
    25741 tccatgtgcc gataaatctg aaagccttcc actgactaag gcctgatatt ttttcacaaa
    25801 cttcttttca cgaagttgca tatctaattt tgcgtgcgca aatccatgct ttgcaaaaag
    25861 catcaagcca gatgtatccc gatctagtcg cgtcaccaca tggattactt ggttttcgta
    25921 accttgtttt ttataataag cctttacacg attagccatt gtcccatttg gatggtattg
    25981 ggcaggaata gacgaaacgc ctgctggttt atttactacc agaacgtgtt catcttcata
    26041 cacgatatcg atcggtgttt catccaaaag tacggtttca tgttctcctt cagcaggaat
    26101 cacaatggtt accttatcat ccttagctag agaaaacaag acattttcta cttgatcatt
    26161 aactttgatt tgaccgcctt gaaattttat tttcgctaac agaccctttg agatcccttt
    26221 ttcttttaaa aaatatttta cttgctgaga atgatctttc tcataatccc atgtaaactg
    26281 catatcaatc ctcactaata aaggcatctt tcacacgatg ccaaaaatgc atatgtcgat
    26341 aagaagcaaa atggatccgt tcttctgcta ttcgataata aattgagcgg atatttgctt
    26401 ttgcgacatt caattgatca accgttacaa gataatcttc tgtattttcc aattttattt
    26461 ctagccattc atcatgggca atcacgatcg gagaacccaa agtacgaaac actcgattat
    26521 tcagtgaagc aatctctgcc aactgaaagg cattgatact aggatggatc acagcaccgc
    26581 cgacactttt attatatgct gtcgaaccag taggcgtaga aatcgtcaat ccatctcctc
    26641 taaaactttc aaacagctcg tttttgatgt atacatcggc taccatcgta cgattggccc
    26701 gtttaatcgt tgattcattc agtgctaaaa aatgctggtc cggtgtttca tccaaataac
    26761 tgatccgaac atccagcaag ggataactaa cactttgttc ccggttcgtt cgtaagctgt
    26821 ctactagttc ttccagctcg taatcccgcc aatccgtgta aaaccctaaa tgacctgtat
    26881 gcacgcccaa gaaacgcaca tcattcagac aatgactgta gtgatggaac gccgacaata
    26941 atgtgccgtc tccgccgact gagatgacca attctggctc atgatcatct cgttgaatcc
    27001 ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc tatagtcttt gcttctttat
    27061 tatgaacgat cgctactttc atcccctgcc tcctctcttt cgctttcttt cttcaaaagc
    27121 gttcgatagt agacatcatc tgattttcct cttccatggg agaataaccg ctgtgcttct
    27181 tggatttctt cacggatact ggacatctct tcatccaact gataggcagc ttctgccgca
    27241 cgctgaagtc gttcactcat ttcttctgga aaaacgcctt gatatttata attcagcgaa
    27301 tgctcaatcg ttgcccagaa attcatcgca agagtccgaa tctggatttc cgccaaaatt
    27361 ttcttctcac cgctgatcag ctgtacagga tactcgatca ccagatgata agaacggtag
    27421 ccacttgctt ttttatttgt gatgtaatct cgttcttgga gaactttcat gtcttttctt
    27481 ttacggatga tctcaacaac ttgatggata tcttctacaa actgacacat gatccttaac
    27541 ccggcaatat cagacatttc ttcttctaaa cgtcccattg gaatatgccg taattgtgat
    27601 tttgttacaa tactatcgac tggcttgact ctcccagtga caaattcaat cgggacatgt
    27661 cgattttgtt cacggaactg tttgcggatc ccccgcagtt tcactttcaa ttctgataca
    27721 gtttgttcat atggtgctaa aaatgcttcc caatctcgtt ccatagctga tacctctact
    27781 tcattaacat tcattgttta ttctaccata tatgcaaagt tttttcgtac tcttgattta
    27841 tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa aaggagtcga tcaaatgaca
    27901 caaatgcttg aaatcgaata taaatctatg ctgacaaagc aagagtacga gatattcatc
    27961 gctcactatc aattaacaga taaagatttc cgtgtacaga ctaatattta tttcgatacc
    28021 gttgatgaac agctgaagaa acaaaattgt ggcctacgga tccgtttcgt tggtcatcaa
    28081 gctgaatata cattaaaaac acctgctgaa aaagggcgtc tcgaaacaac ggataccttt
    28141 ataacagaag aagcagaagc atttatcttc gaaaaacgtc tgcctcgttc aggcgcagtt
    28201 tttcaaaaac tttctgattt gaatatcgat cctgcttcat taatccaaac cggaaagctt
    28261 acaacaaaac gagctgaatt ccctattgaa gaaggacttt tagctattga tgaaagctgg
    28321 ggagacaatc tacacgattt tgaactggaa ctagaggtag gggatgcatc tgtgggaaaa
    28381 attgctttta taaatttttt aaaacgtttt tctttacctt atcgtcctgc taagaacaag
    28441 atccaacgta tgcttgaagc taaaaattag aaacctttca ttttcacaag gcacttgagt
    28501 tcattttttt acttttttct gttaagttat aggatgtgaa agaatctagt cactagttgt
    28561 atagaatcta ttttggggga gattcatatg atcgaaatct atttatttgt taatccttta
    28621 ggggaatact gtttcgaaat ggaacaacag ttattacaat ttatcgaaga agagtacggg
    28681 acaccatcaa aagaaaagat gcaatttcgc tttcttcctt tggtcaattt gcagacaatt
    28741 ggggatgtca tgcaacaaaa aggaatttct cagaacaatc ttgaggaacg taatcactta
    28801 ttctcaatga cttattctgc agcattggac tgcaaagcgg cgcagtttca aggaaagaaa
    28861 aaaggccgcc gttttttgat caaattgcaa gaagcagtcg gctgtaatga cataccatat
    28921 tctcaagaac ttgctgaatc catatttgaa gaaatcggcg gcgatttgaa tatgtttaaa
    28981 gaagatcgtc agtctgactt cgttaaagaa gtattctttt ctgatcaaaa aattgctcgg
    29041 gaaatgggca tcacaaaaca cccttctgca gtcgtttaca attatgcctg cgaccgtgat
    29101 tttggtgttt tagtagaagt aaaaggtaca agtagccttt ggagaatcac agagctgtgt
    29161 aaaacaacag ataactcata tcaagttttt aatggtgaac atttacatac aaatgattct
    29221 cagttccatt caagaactag ccatttacac ttgctttcta atta
//
LOCUS       DACDNO010000036        28792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDNO010000036 DACDNO010000000
VERSION     DACDNO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.036"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(56..643)
                     /locus_tag="IWI22_001618"
     CDS             complement(56..643)
                     /locus_tag="IWI22_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169011.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            818..2140
                     /locus_tag="IWI22_001619"
     CDS             818..2140
                     /locus_tag="IWI22_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9169012.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2295..3071
                     /locus_tag="IWI22_001620"
     CDS             2295..3071
                     /locus_tag="IWI22_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9169013.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3064..3633
                     /gene="rnmV"
                     /locus_tag="IWI22_001621"
     CDS             3064..3633
                     /gene="rnmV"
                     /locus_tag="IWI22_001621"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9169014.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3626..4510
                     /gene="rsmA"
                     /locus_tag="IWI22_001622"
     CDS             3626..4510
                     /gene="rsmA"
                     /locus_tag="IWI22_001622"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9169015.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            4961..5590
                     /locus_tag="IWI22_001623"
     CDS             4961..5590
                     /locus_tag="IWI22_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169016.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="IWI22_001624"
     CDS             complement(5714..6823)
                     /gene="ugpC"
                     /locus_tag="IWI22_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9169017.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7032..7454
                     /gene="mgsA"
                     /locus_tag="IWI22_001625"
     CDS             7032..7454
                     /gene="mgsA"
                     /locus_tag="IWI22_001625"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9169018.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7777..8313
                     /locus_tag="IWI22_001626"
     CDS             7777..8313
                     /locus_tag="IWI22_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9169019.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8353..10116)
                     /locus_tag="IWI22_001627"
     CDS             complement(8353..10116)
                     /locus_tag="IWI22_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169020.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10119..11834)
                     /locus_tag="IWI22_001628"
     CDS             complement(10119..11834)
                     /locus_tag="IWI22_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169021.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(12613..12837)
                     /locus_tag="IWI22_001629"
     CDS             complement(12613..12837)
                     /locus_tag="IWI22_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169022.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            13037..15805
                     /locus_tag="IWI22_001630"
     CDS             13037..15805
                     /locus_tag="IWI22_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9169023.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            15886..16305
                     /locus_tag="IWI22_001631"
     CDS             15886..16305
                     /locus_tag="IWI22_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9169024.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            16317..16787
                     /locus_tag="IWI22_001632"
     CDS             16317..16787
                     /locus_tag="IWI22_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9169025.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            16804..17583
                     /locus_tag="IWI22_001633"
     CDS             16804..17583
                     /locus_tag="IWI22_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9169026.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            17570..18391
                     /locus_tag="IWI22_001634"
     CDS             17570..18391
                     /locus_tag="IWI22_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9169027.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            18408..19457
                     /locus_tag="IWI22_001635"
     CDS             18408..19457
                     /locus_tag="IWI22_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9169028.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            19470..20474
                     /locus_tag="IWI22_001636"
     CDS             19470..20474
                     /locus_tag="IWI22_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9169029.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            21107..22126
                     /locus_tag="IWI22_001637"
     CDS             21107..22126
                     /locus_tag="IWI22_001637"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9169030.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            22169..23050
                     /gene="galU"
                     /locus_tag="IWI22_001638"
     CDS             22169..23050
                     /gene="galU"
                     /locus_tag="IWI22_001638"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9169031.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            23350..23727
                     /locus_tag="IWI22_001639"
     CDS             23350..23727
                     /locus_tag="IWI22_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169032.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            24333..24947
                     /locus_tag="IWI22_001640"
     CDS             24333..24947
                     /locus_tag="IWI22_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9169033.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            25720..27459
                     /gene="yfcC"
                     /locus_tag="IWI22_001641"
     CDS             25720..27459
                     /gene="yfcC"
                     /locus_tag="IWI22_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9169034.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            27616..>28792
                     /gene="iadA"
                     /locus_tag="IWI22_001642"
     CDS             27616..>28792
                     /gene="iadA"
                     /locus_tag="IWI22_001642"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9169035.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     "
BASE COUNT         9330 a         4938 c         6000 g         8524 t
ORIGIN      
        1 aacaatcatc ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact
       61 tgattttatg gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca
      121 gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat
      181 agagctttgt caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa
      241 accaatgatt atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat
      301 aaaaatagat cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata
      361 aaaagaaaat cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat
      421 acaacatccc ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca
      481 aagtcagcaa tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta
      541 atgtgaacca atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg
      601 ataaataatc tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg
      661 tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga
      721 gtttttcact gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga
      781 aaattatacc aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga
      841 aatcaatctg tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt
      901 cttcaaaact gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg
      961 gcttttgggt ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc
     1021 aatcccggaa actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag
     1081 ctttttatta ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc
     1141 gattatcttt gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt
     1201 gattgcagtg atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg
     1261 tgcttctgta caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat
     1321 atggggatta ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa
     1381 agatgttact tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga
     1441 cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc
     1501 taaagcaatt attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt
     1561 ttttttgaaa actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc
     1621 atcagacgtc atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc
     1681 ggtatacggt gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc
     1741 tttagaagac gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt
     1801 tccttatgtt cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc
     1861 tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt
     1921 tatcggtgtc tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc
     1981 actttatcca atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac
     2041 gatgatgacg caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc
     2101 agtctattat caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag
     2161 aaaaagtacg aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt
     2221 cttctatttt gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa
     2281 aaggagagat aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg
     2341 aagatattcc agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg
     2401 tgggcttcga tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata
     2461 tatacagtat tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa
     2521 ataaattaca agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg
     2581 attatcattg gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg
     2641 ctatcgcaaa agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca
     2701 cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca
     2761 gtggtgatta tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg
     2821 gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg
     2881 aacgattact tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc
     2941 gaaatgaacc aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc
     3001 cgacagaaga agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg
     3061 aacatggata agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga
     3121 aggctgcggg aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa
     3181 gagatcctga tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat
     3241 cctgattttt caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa
     3301 catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag
     3361 catgcaagtg atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc
     3421 caaacggctg tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg
     3481 gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac
     3541 ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa
     3601 gcaatgaaaa tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag
     3661 aacaaaagag attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt
     3721 tctgactgaa ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac
     3781 gaacgtgatc gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc
     3841 aaaacaagtc gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat
     3901 gcaaccctat gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac
     3961 agctgttaga gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta
     4021 ctatattacg acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact
     4081 agtcgtcatg atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc
     4141 ttatggttca ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt
     4201 accaaaaaca gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag
     4261 aagagatata ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc
     4321 ggcatttcag caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga
     4381 tgaccagaca aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg
     4441 tcgtggggag actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa
     4501 tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa
     4561 taagaacagc caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttattaccgc
     4621 agatgcaagc ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa
     4681 gtcacaagga acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta
     4741 cttgatgcaa gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc
     4801 acatctccta tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt
     4861 tgtatggatt tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag
     4921 tcgacattta tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg
     4981 aaaactaacg atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc
     5041 taaaatcatg attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc
     5101 gatgtttttt acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt
     5161 tttaacaacg ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat
     5221 tggggcttgg tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt
     5281 tgctaattgg cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga
     5341 aatgctagta gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca
     5401 aggatatttt tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt
     5461 tgattataat attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt
     5521 atttacggca gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac
     5581 gacatattag acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc
     5641 tctaagatga gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct
     5701 aacagggggc aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc
     5761 ctttgctgat gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat
     5821 ctacttttga gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa
     5881 gtaattcgga aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta
     5941 cttgttcact gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac
     6001 ctttttcaac taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat
     6061 ctgtgataac accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa
     6121 agccggcaac gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt
     6181 ggataaatcc gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat
     6241 cgtgagtaac atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac
     6301 gcatggctac acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt
     6361 ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag
     6421 cagctggttt tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac
     6481 gttttttgat gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa
     6541 aaacagtcat atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt
     6601 taggtgccac gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt
     6661 ccagtcccgc gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa
     6721 cgataaattc acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat
     6781 tgtcatattt tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt
     6841 cttttttagt tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc
     6901 atgttgaaca agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat
     6961 atccataaga aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag
     7021 gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga
     7081 aattagcaac agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga
     7141 ctgggcagag gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt
     7201 taggcgggga tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt
     7261 ttcttcgaga cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt
     7321 tatctgatgt ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa
     7381 gaggactgga agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc
     7441 cactttcatt ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta
     7501 gctataaaag ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata
     7561 aacaataata ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg
     7621 ggaaaagatc ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg
     7681 gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat
     7741 gatacgatgt agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca
     7801 cgtatgattg ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta
     7861 gcaatcgaga ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc
     7921 attctttttg gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg
     7981 gccctcttct cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga
     8041 gcaatctacg gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct
     8101 acgcttagtg tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg
     8161 taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata
     8221 tggttaccga ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa
     8281 attttaagaa gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa
     8341 gactgcttgt ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc
     8401 tttttgaagc agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa
     8461 tatctggtcc gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc
     8521 ttccatcatt gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct
     8581 ggtagcttca tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa
     8641 cagttggcgt tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt
     8701 ttccggcaaa cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc
     8761 tgcgtcactt gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag
     8821 ccaagtatct tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg
     8881 gatgtcatgg tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa
     8941 attaacaagt gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg
     9001 ataagcagta aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac
     9061 atgatcaaaa gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa
     9121 atcaggcttt tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc
     9181 ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga
     9241 gtagatcgta aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa
     9301 taggccgcca actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg
     9361 gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag
     9421 cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt
     9481 accgattcgt tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa
     9541 atttgtttgt gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca
     9601 aagaacgacc aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat
     9661 gattgccact ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc
     9721 catatcattt gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag
     9781 tggcaatcga ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc
     9841 taaccgattg cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc
     9901 accgaataga ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat
     9961 ttgatcgata ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat
    10021 tcctagtatc acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg
    10081 gcggagcgtc tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg
    10141 acgatttcct gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca
    10201 atcttacctt gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg
    10261 gagataataa tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct
    10321 gtttgataat ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc
    10381 aaagctcttg caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc
    10441 atgacttttg tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt
    10501 ttcagagctt cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca
    10561 cgaatagtcc cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga
    10621 tgatccagct gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca
    10681 tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg
    10741 attccaagga ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa
    10801 tcaggagtat aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg
    10861 ggagtagtac tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc
    10921 cgatttcctg aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt
    10981 gccaaaagca tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta
    11041 ccaatattta ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc
    11101 aaggttgtag caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct
    11161 agatcatttg tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg
    11221 atgacacgaa caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct
    11281 tgcacttttt ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa
    11341 ataggcaata atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc
    11401 actgacccga tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct
    11461 agctgaagct gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga
    11521 ttcaactcgt tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta
    11581 ccaaatcctt gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca
    11641 atcacagaca tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt
    11701 tttagtccat tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct
    11761 tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt
    11821 aataactgaa tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta
    11881 ctaccacatt gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga
    11941 aagttttatt atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa
    12001 atgagaaaac atgccagtct cttcattcat aggaacgtct attttactta atttttctat
    12061 aaatgaattc tgcataaaaa atatatcaag taaatgttta tagatgaaac tctatttact
    12121 tcttttttaa tttgttcttg caatcttttt tgctacgtaa tttttgtaaa aaagtgggtt
    12181 gagagaaatc taattgattt tcaaagaagg agtatgaaaa tgagaaaaat atttattcgt
    12241 ttattgtggg tactatctta gaggaataaa taatagtgga gaaaactatg gtttggcgca
    12301 gattctatca ggtttgatgg aacgaagaca acctcccgac atcaagtcaa aagctacttg
    12361 agattttgat ctgaaaataa aaaaagaaaa caaggtagga acatcaatgt ttctaccttg
    12421 ttttcgtaat caatgggaaa aaactaagag tggtgtacgt aatcaataaa aaggatggtg
    12481 acaacagtac ctctttactc tctgaaagca tcataactcg tttttcctca ttatgccaat
    12541 aaattgttgt tataccacat ataaggattt gtaatagcta aaaacagaca ggaaagttga
    12601 aatctgtgaa tcttattttg tatggaagga tttatagaac gcaaaaatca agataaagat
    12661 gaataaaaat cctgtttcgg ctatatattg cgtatctacg ccaataaaac gagtttgttc
    12721 tgtcatagcc atataaagca aaggcaaaaa tacaactaca gcgagataag atccgatgat
    12781 ttctcgaaaa gttttccagg aagtatgttt cctgttttta tatttatccg atttcattga
    12841 atagtcatag cctccttatt ctttcatatg ttgaaattcc agtataaaaa ctggaaggaa
    12901 ctgtacgttt tttagaataa aaattataag ggataagtag gctgctgtct ataataagat
    12961 atatttttca attctttatt aaagatttta ttgacaaaat gaatccgttt tctatgatgg
    13021 aggaggggtg aataagttgt tgaagaagga aattttatgt tacttagaga accagacagc
    13081 ctttttcgat ttagaacata tgaacgaggt ctttacagca caaaggttag ctgatcattt
    13141 tggggtaaaa agaaacacag tcagtcatta tttgaaccag ttaaatgatg agcaactact
    13201 agtcaaaatc aattctcgac cagtgattta tttccacaaa gaagcttttg aaaaacaatt
    13261 ttttaagtta cgcacgaatt tttatttttc tatcgatgat ctcaaagagg aacagccttt
    13321 tttcgcgcaa ccaaaagact tattctcttt tatgatcgga tacgacgcca gtttgaaaga
    13381 aagtatcgaa caaatcaaaa cagctcttta ctatccagat ggcggattac cacttttgat
    13441 tacaggcgaa agtggcacag gtaaaagtta tttagttaat ttggtctatc agtattgtct
    13501 gcttcatgat ctgctagaag attctgcacc attcatcact tttaattgtg cacaatatgc
    13561 agataatcct gaacttttga ctagcaattt gtttggacat gttaagggag cgtacactgg
    13621 tgcagaagaa aataaaaaag gggcttttga agcagcagat ggtgggatct tgtttttaga
    13681 cgaggtacat cgcttaacac cagaaggtca ggaaaaactg ttcacctatc ttgatcaagg
    13741 tgtgatttat cgtatgggag aacctaattt gtcacgccgg atcaaaacac gtctcttttt
    13801 tgctacgact gaagagatta ctagtcattt cttaacaact ttcattcgaa gaatacctat
    13861 ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat gaacggctag aactgattta
    13921 ctcttttttt ctcgaagaac aaagaaaaat ggggagccct ttgtcagtca gtggaccagc
    13981 tttgtcactt ttaacaagtc agaaattatc cggaaacata ggagagctga aaaatatcgt
    14041 caaagtttca gcagcaaaag catatgctga acagcgtgaa cagcctgaaa tccatgtcac
    14101 gatccatcat ttgcaaaaag aactacttgc gcagcctatt actaaaaata cagcacagca
    14161 aggaatctat attacgcaag accagacctt agagcaatta atggagaagc aacagcccga
    14221 acagcagcga atcgttaaaa gttttgagca aatcctaatt gatttcaaga aaaataactg
    14281 tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg attcaattat ttgatttttt
    14341 gctgtttgag accagtagac aagaaaaaca cgagctgctg gtttattgga cacaatctat
    14401 ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc aaattcgatg gaaacagtgt
    14461 ctatgccttg agttactatc tctatcagcg aagcactatc aaatggttac cggaagaaca
    14521 tgatattatc caactaatca aagaattaga acaacaagtt tctcagtctt atccatctag
    14581 ttatcactat gtccaacgaa ttctggaatt aagtaagatc aaattagata ttgaagacac
    14641 aagtatggat cggattttac tgacgatcta tctaaaaaaa gcgaaatggt caaaagaaaa
    14701 acgactgcca aaagcgatga ttgctgctca cggttatgca actgctagca gtatggcaaa
    14761 tgtagtaaat cgtttattac aagaggatct atttgaatca tttgatatgc cactagatgt
    14821 cacaccacag cagattgctt cagaaatcct tgattattgc gaaaacagcg atgtttcaaa
    14881 tggattgatc atattagtag acatgggatc tctgaaagaa atccatcagt tttttaaaaa
    14941 acagttatcc gtaccgcttt tgattttgaa taatgtgacg acacctttag caatcacagt
    15001 aggggaatgc ctgcaaaaaa atgtatcatt agaagagata gcagaagaga ctgttcgaca
    15061 gatccagcca gaatggcgac tgctctatcc agaggaaaac aaacccaaag cactcattac
    15121 tacttgcttc actggaatcg gtacggcggt ccacttaagt gaactgctag aaaaaagctt
    15181 acctacggct tgtcagttga aaatcatccc atatgaatat cagcaattaa aagataagaa
    15241 aaacagtgac ccattatttt ccatatacga agtcgtagga atgattggca ccacagatcc
    15301 tgatatcacc aatatcgact atctttcatt agaagacctc atctcggggg aaaagatgtc
    15361 tgtactagct gaatggttgg acactactat gaatgcttca gagaaggaaa tattcaatca
    15421 gcgcatcatt cgtaattttt cattggaaaa agtactggat tccgtgacta ttctagatac
    15481 agagaaggtg atgggagaaa tcgatcattt tatgagagaa ttagaggtgc agttgaatcg
    15541 aagcatcagc aatcccaaaa aattagcgtt gtatgtccat gtgagctgtc tcattgaacg
    15601 attgatccga caaatgccaa tcgaaaccta tcaaggatta gagaaatgga agcagtgtca
    15661 aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc attgaagagc attatagtgt
    15721 catgatcccc aattctgaga ttgcctatat ctatgatatt ttgagtgaag atacagagtt
    15781 tctctcgata gaagaagaat tttgaacata agcaagttgt tgccactaat tttggcatgc
    15841 aacttgcttt tttagtatgt gtaagtgaaa agaggagatg aaaaagtgga tagagaaatt
    15901 atcttagctt cccatggaaa atttgcttca gggatattag attcattaga gctgatctat
    15961 gggaaaaatc attctattac tgtattcgat tgctatactg atgagaattt tgatttagcg
    16021 gaaacagtaa ggcagctttt tatgaggtac aaagataaag aaatcattgt tctgacggat
    16081 ctttttggcg gaagtgtcaa caacgaattc ttacaatata tcaatcggga tcatgtctat
    16141 ctcgttgccg gattgaattt gccactattg attgagctag tatctcggtt ggacgctgaa
    16201 gtagagacaa ctgttttgat ccagcaggtg ctaactgatt caaaaaaaat gatccagttt
    16261 tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat tttaggagga agaaaaatga
    16321 ttgtattgac aagagtcgac catcggttat tacacggaca agtagctttt tcatggacac
    16381 aaacaatagg cgcagactgt attttgattg ctaatgatga tgtacctacg aatgagatac
    16441 gaaagacaac gatcaaatta gccaagccac aaggagtcaa actagtgatc aaatcaattg
    16501 atgactcgat tgccgcgttg aaaagcgggg tgacagataa atataagtta ttcatcgtag
    16561 tggaatcgat cgaggatgca tataagctgg cagaagcgta tccagaaatc aaaaccatca
    16621 acttaggagg gatcaaggca aaacctggta ctcgcagcat cggaaaagcg gtaaatattt
    16681 taccagaaga ggaggaacgc ttgaaacgat taattgacaa acaaatagaa gtagaaatcc
    16741 gtcaagtacc agcagataaa aaagtggatg taagagaagt tttgtaagaa aggaagaacc
    16801 tcaatgattg tacaagcagt cttattagga attgttgcat ttgtcgcaca atcagaatat
    16861 gcactaggga catcgttgct ttctcgtccg attgtgacgg ggctgtttac aggaatcgtg
    16921 ttaggtgatg taaaaactgg gatcattatg ggcgcaactt tagagttagc atttatcgga
    16981 tcgttctcag taggtgcttc gattccacct gatgttgtga ccggtgggat tttagggacg
    17041 gcttttgcta ttacagctgg agccggtaca gaaaccgcgc ttttactagg tttgccgatt
    17101 gctacgttga cattgatatt gaaaaatatt tacttagggc ttttgattcc aattatgaac
    17161 cataaagcag acaattatgc agaagaagga aattataaag gagtcgagca tatgcacttg
    17221 cttgccggta tcggtctttc cttgatgctt ggtgttgtgg taatggtttc ttatatggtg
    17281 ggaagcaata cgatcggaaa tctattaaat atgattcctg acttcgttca aaaggggttg
    17341 tcagtagcta cagggttgat tccagctctt ggttttgcga tgcttgctcg tttgctgatc
    17401 aacaaacaag ttgcaccata ttttttctta ggttttgcta tagcttctta cttagagatt
    17461 cctgtgaccg gagtagctat ttttggtgcg attttagcag ttgtggtagt caatattatg
    17521 aatgtccgtc aactaccact tcaaacaact gcaggggagg tagaagaaga tgaagaattc
    17581 tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat cacgtatttt ggcgttcttt
    17641 tcaaatggag ttttcttgga attatgaacg tcagatgaat atggcttatg tttatgcaat
    17701 gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa atggcagcag ctttgaaacg
    17761 gcatctagag ttttttaaca cgaccccaca tatcgttaca ctgattttag gaatcacggc
    17821 tgcaatggag gaagaaaata aggatgatcc tgaatttgat gtgactgcga tcgatagtat
    17881 caaaacatct ttgatgggac ctcttgcagg gttaggagat tctttctttt ggggaacttt
    17941 gcgattgatt gccacaggtg tgggtacctc tttagccatg caaggaaata ttttaggccc
    18001 tatcttgttc attttgattt tcaatattcc gcacattctg tttcgctatc tagcaacagg
    18061 ttggggatat cgcttaggaa cgggtttttt gaaaaagatc caggcaaacg gtatgatgga
    18121 aagtttgaca ttaggcgcgt ctattattgg actgatggtc gttggagcaa tgactgccac
    18181 gatgatcacc atcaatattc ctttaaaaat cggttcagga gaaaatgctg tgactgttca
    18241 aagtattttt gatgatatcg ttccaaatat cttatgtctt ggagcatttg gcatagtgtt
    18301 ttatctattg aagaaagaag tgaaaccatt aacgatcttg atcggactag ctttctttgg
    18361 aatttttggt tcgtggatag ggatttttta aaaaaggagc agagaaaatg tttacgatgc
    18421 aagattatat ctatgaagaa aaggaagtac taagtacgat cttgaaaaag aatgattttt
    18481 cgacaagaga acatatgaaa aaaacagtga atttactgat cttagcaacg ggttcatctt
    18541 ataacgcttg tttagcagcg aagccagcat tggaatctta tggtgatctt actgtcgata
    18601 ttcaagaacc tttttatttt ttcaattatg gaaaactttc tccttcaatc gatactgtga
    18661 ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga agcagtaaaa atgatacaaa
    18721 aacaagggtt acctgttgtt gctatcacaa atgacgtgca aagtccactt gctttagaag
    18781 ctgcacaaat tattgattta ggtgccgggg tagaatcagt aggcttcgtt acaaaaggct
    18841 attctgcgac tgttttacag ttactactct tagggatagg aattggaata agtaaaaata
    18901 aaatcagcaa aaaaatagaa caagactaca tgcaacagtt gcgcaaaata atcaaccatt
    18961 taccagcgat tattcaaaaa acggaagaat tttttgatgg atatcaaagt ttatttcgtt
    19021 tagcacagcg tttcgtcacc attggatatg gtccaaactg gggaacagct aaagaagcag
    19081 agacgaaatt gactgaaaca atccgcgttc cgtcacaagg atttgagtta gaagcttata
    19141 tgcacggccc atacctggag gcagatgctt cgcatttgct attctttatt gaaggggaaa
    19201 gtgtaaataa ggaacgttcg caaaagttgc aaagatatat gagtcggtac gttggagaga
    19261 ctctcaccat taccacaaaa aaagcaagaa acgagaaaac acttggatta gcaatagaat
    19321 gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt tcaactattc gcttataaaa
    19381 ccgcagtagc taaaggtatt gacttgaaca aaaagatttt tgaagacttt gatacagtct
    19441 tgaaaagtaa attataagag gtggaaatca tgctgaaatt caatgaagaa gaacaaatca
    19501 aagcaataaa aggcgcacta gctttacgtc cgcaagtaga agaaatcatc gataaacttt
    19561 atgcggaaaa atttgacgct gtctattatc taggaatcgg tggaacgtat gcttcctcga
    19621 tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc tgtctttgta cagcatgctg
    19681 cagaatatta cactactgga aacaaaagat taacaaaaga ttctatcgtt attttgtcct
    19741 ctgttaccgg tacaactcaa gaagtagtga aagcagtaga acaaatcaag aaagtaggag
    19801 cgacactgat tggatttatc gataaagcga acagtaagtt gtctcagcta tgcgattttg
    19861 tggtcactta tccagctcct ggaacagaac aaatcaaatt ttttatggca gcagatcgat
    19921 tgatgtattt gaatggagaa ttcgaagctt attcagaata ctatgaacag ctcgaacgtt
    19981 atttaccaac agggttagtg gaagcagaga aaaaagcaga tgcatttggc ttatcatttg
    20041 cagaaaaaca tcgacgtgat agtatgcatt attttattgg agcagggaat caatggggag
    20101 ctgtttattc atatgccatg tgttactggg aagaacaaag ctggcttcca tcgaaatcta
    20161 tccatgctgc cgagtttctt cacgggacgt tagagatcgt agaagaaaca accccagtca
    20221 cattattttt aggcgaagat gaacagagag tgttatctga acgtgtggct aaacttttgc
    20281 caaaaatttg cagcaattat acattgatag acacaaaaga ctatccagta gaaggtatca
    20341 gtgaaaaata tcgcggacgt gttttgtctt tcttattgat gcatgtagtg acacaacgca
    20401 tcgatgctca tgtggagcag ttgaactgcc atccattaga gatacgccgc tactatcgtc
    20461 agtttgatta ttaggataga ctggataaaa agaatataaa aaataaagag agcagcgata
    20521 actgctccct ttattttttt aaaatattgg catcaattac gttataaaac acattttttg
    20581 cattatctac agttcaactt ataatctgtt taatgggaca tgtgggtgcc aaccattttt
    20641 tgttatgcaa tagtactatt tagttgttgc atggatccgt agtaaaagtc caagtacaac
    20701 gagttgcgcc agtttacata gcgttttgta tccagaatgt tgactaaaac aaaagttcct
    20761 atgtcaaccg tcaacagaga caatctgtcc gttgcaggac ctgacagatt tagaaatgtt
    20821 caatctgtct cttatgtatt ttaaatcaat actcactagg agaagtttcc taacctccta
    20881 atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc ttaggaacaa gcaaagcata
    20941 acgagtgtaa aaataaaatg tagactattg taacatatca ttgattattt caagtaaaaa
    21001 cgataaaaga agtaaacaaa cacttaaaaa caatggaaat tagtcccaac tttaagaaat
    21061 gtatactata atggaaatta tcaattattt atttggagga atatccatga aacaaaaaat
    21121 cgcagttctt ggtcctggat cttggggcac tgctttagca caaacattgg cagaaaacgg
    21181 gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt gatgaaatca ataagtatca
    21241 tacaaatcag cattttttac ctgatttgac gatcccagaa tcgattatcg gatataaaga
    21301 attggcagaa gcagtggata acgcagatgc tattttattt gttgttccaa caaaagcgat
    21361 tcgttcagtc gcacaggaac ttattggtaa aatgaatact aagccaatca ttatacatgc
    21421 aagtaaagga ctagaacaag atacgcataa acgaatctca gaagttttgg ctgaagaaat
    21481 tccagaagaa aaaagacagg caattgttgt attatctggt cctagccatg ctgaggaagt
    21541 ggcagtacat gatatcacaa caattacagc tgcaagtatt gatcaaaaag cagcagccta
    21601 tgtacaaaaa ctgttcatga atgattattt caggatttat acaaataatg atgtgattgg
    21661 cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt ggtgccggcg ctatccatgg
    21721 attaggattc ggagatgatg caaaagcagc aatcatgact cgaggattgg cagaaatcag
    21781 ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt atcggtttga gtggtgtggg
    21841 ggacttgatc gttacttgta caagtgttca ttctcgcaat tggcgtgccg gtaacttgtt
    21901 aggacaggga cataagctgg aagaagttct agaaaacatg ggaatggtcg ttgagggtgt
    21961 ggcaacgaca aaagcagcag tagagctagc tcaacagcta ggtgttgaga tgccaattac
    22021 tcaaacaatc tataatgttt tgtataatgg agaagatatc aaaaaagcag caaaagaaat
    22081 catgttacgt gacggaaaaa tggagaatga attttctctt cgttaaatag atgatcaata
    22141 gttaatcgtc gtttaggagg aacagcaagt gaaggtcaaa aaagcagtca ttcctgccgc
    22201 tgggctagga acacgttttt tgccggcaac aaaagccatg gcaaaagaaa tgcttccaat
    22261 cgtggataaa ccaacgatcc agtttattgt ggaagaagca ttggattcgg gaatcgaaga
    22321 catcttgatc gtaacgggaa aagaaaaaag acccattgaa gatcactttg atgcaaatat
    22381 cgaattggaa aacaatttgc gggaaaagga aaaaacagaa ttactcgctt tagttgaaga
    22441 aacgactcaa gtcaatcttc acttcatccg tcaatctcat ccaagaggac tgggggatgc
    22501 cgtgcttcaa gcaaaagcat ttataggaaa tgaaccgttt gtcgtcatgc tgggggatga
    22561 tttgatgaaa gatgatatcc cattgactca acaattgatg atggattatg aagaaacaca
    22621 agcttccgct gttgccgtca tgcgtgtacc agaaaatgaa acatctaaat acggaattat
    22681 tgatcctaaa acagatagcg aaaagggtcg ttgcagagta aaagggtttg tagaaaaacc
    22741 tgaaatggga aaagcaccaa gtaatttagc cattatcgga cgttatctac tgactcctaa
    22801 aatatttgag atattagaaa ctcaagaacc aggagcagga aacgagatac agctaacgga
    22861 tgcacttcag actcttaacc agacagaggc ggtctatgcg agagagttta aaggaaaaag
    22921 atacgacgta ggcgataagt taggatatat gaagacaaat attgaatatg gacttcaaca
    22981 ttcggaaatt gggacttcat taagtgcata tattatccag ttgagtaaaa agctaagaac
    23041 agaaagttaa ctggaaataa gtggcctgct gagaatgaaa ggctatttgt tattaaagcg
    23101 gcagcttgtg cgtcatttgc gcaactgtcg ttttagtttt ttgactctag atcagttgag
    23161 tcgtggaaaa cgaaagatag aaaaagactt tcgtgaatag attcagaaaa tattatgcta
    23221 atggtttaat ttttttttaa gttgtataaa agaaaatgat tacataagag aaatgaaagc
    23281 caagccagaa gattcatagt agaataaaaa ggcgaatttt ttaattataa aaaaaggtgt
    23341 gtgttcacta tgggaaaatt agtcgctgtt gcacagtcgc tgcttgatat actcaagtgg
    23401 ctgatggaag gaagcaattg gatatttgtg ttgccactat tgcttattag tctatttatc
    23461 atgtttcgca tggtcagaaa aaaagaaacg tttctgattt ttatctggta tgcagcatcc
    23521 gttggggttg ctaccttgat ggttgaattg gcgttaggta gttgggaagc ttgcgtattt
    23581 gctttatttt ttgctgtact gtatctaatc atttatatcc catatatgaa aagaaagtct
    23641 cggataggca gaaaaggtag acatacaact actttttcta gtacagattc tctcgaacaa
    23701 agtagaagaa cgagaagaag acggtaatat ttagtaggta tacgcaaaaa aagataaaat
    23761 aatggataaa ctattgagat gagagattta tttaattggt ggaaaaaagc ttttttcact
    23821 tatataaaag aatctttttt aaaggaatgc ttgttgtatc gggataagcc attccttttt
    23881 ttatgttatt tatatatata taaatcatac caatgttttt ttgtattttc ttgcttgcac
    23941 ttggataaga gattacttac aataaattca gatagaaaca gctgaataaa aaaaggctcc
    24001 ctgaccctta gttggcgcta agaatcaggg aatgaccagt cacacacata caccctgacc
    24061 gatcattttg ccatagtcaa tgcagtgcat cgacctaatt tattgtacct aatctagggt
    24121 gaaaattcta cccctatgta gatattttcc atggtctgca cgttgtttta tttgctatgt
    24181 caaggtattg ggcgtattcg ttcaatgcct tttttgtgtt tgaaacagca aagaccacat
    24241 gtgttactgt ctaactactg tttttttatg tacttcctta gattaggcag ctgtttctat
    24301 tcatgtcaag tatagctagg cgggagagat aaatgagttt atgttatttt gactattatc
    24361 accaattgga agagaaatta aggatagaag agccgctgat gcttcaagct ggcacttgga
    24421 tcgcgaaaca aattaatcaa ggtggtagat tgcagatata tgctagccgc acactaagtg
    24481 gtgtagcatt tgaattttgg gatcaaaccc cggatcttgt accaagtata ttgattgaaa
    24541 atccagccgg aggtgtctat gaaacgctag aaggtgcagg acaggcaatt attgatcagc
    24601 tttctgtcaa atcagaagac atttttcttc ttctttcgaa tgaaggaaga aacccggcaa
    24661 tcatcgaact agctcagtgg atcaaagaaa atggtcatcc attaatcatt gttactggat
    24721 ttgatttgtc acgaagtatc aaatcgcaac atagcagtgg tttgcgatta tttgaatttg
    24781 ctgatttggt tttggacaac cacgccaatt tacatgatgc agtcttgact ttgccaaatc
    24841 tagatccagc gatctgcgga acagcctctc ttgcaacgat ctttttgctg cagcaagttt
    24901 tgtattttac agcaaaacag ctagtccagg acacaagtgg aaaatagtta tccacatgtt
    24961 gacaaagttc cttaaaaaaa taatgttcca cataaaaatg gagcgtttga aaaaaagaaa
    25021 acgattatta atgaggatta acaacgacta ctttttttgt tttagttatt ttaagatgga
    25081 tcatctttga ttttaagctt gttttgttta aagtttgtta tttttataag ttgtttcaca
    25141 gttatgagaa atgagttata agaatatgta gtgtcagaaa aataaaaagt agtggaaaaa
    25201 gagacaagaa agaatccata tggattcttt cttgtctctt aatacacgtt gatgaaagag
    25261 tcttccccaa aactctttac aaagtaatta tagtacattt gttatcatta ttgcaatgaa
    25321 gtttacctga tataacaaaa ttgtccaaca ggataccttt ttctaataga aataaaatac
    25381 acaaaaattg aaggatagat aatggatatt tataagtgtt tattattttt tttgcaatga
    25441 aattcaattt ttcaatgatt gtaaaatgaa aaaaacagga cgagattttc tctaatctaa
    25501 aaataaaaaa agaattttaa ggaaaaacag agaaataaaa tacttaatat aaatggaaag
    25561 agaaaaaaat aatcatacat aggagaaaaa aaacaaaata gaaaaaagtg attttctaat
    25621 aaattattca tgtggaaatg tcatttttac tgtataattc atttgattat ccatttttta
    25681 ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac aaattcttca
    25741 ggaaaaccga aacgcgactt cactaaatta tcgacacccc atacgtatgt cattattttt
    25801 ggtgtagtca tctttgcatg gatactgact tttgtcgttc cagcaggaaa gttcagcacg
    25861 caagatattg aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt tttacgtcaa
    25921 gattccttcc gttatgctta cgagctggat aagtcgtatg tgtttgatca attagaagaa
    25981 ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg attggaaaaa
    26041 gtcattgctg acggagaaaa aaatcttacg caggaaaaac tagatgaaat ctccttgaca
    26101 gatgatgtgc tttacgatca gtacggcgaa aatatctatg atacttctaa aaaattgcat
    26161 aaaacagcca aaatatgggg aacagatgat tttggcggct ttggtttctt aaattttgtt
    26221 tttgaaggac tagtatctgg agataagtat ggatctgctg tagggatcgc tgcattgatc
    26281 cttgttgttg gaggtgcttt tggtattatt atgcgtaccg gagcgatcga cgcaggtatc
    26341 tatgcgttta tcagtaaaac taagggactg gagcgtttag ctttaccgct attattcttt
    26401 gctttttcat tcggtggcgc tacattcggg atggcagaag aggtaattcc attctcaatg
    26461 gtgatggttc catttgtcat tgcgttagga tatgactcga tcgtagcagt aaccgtgact
    26521 tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag cgtagcggtg
    26581 gctcaaggga ttgctggaat ccccgtgttg tctggtgcga cattccgatt gattatgtgg
    26641 gtagttgtta ctgcattagc cgctggttat atgatgatat atgcagaaaa aatccgtaaa
    26701 aaaccagaaa gttcgttgac ttacaaatca gatgattatt tccgttctca tatcaaaaag
    26761 acatctgatg aaaataaaaa attcatgctt gggcataaat tgatcttatt agagatgtta
    26821 gttgtccttg tctggatcgt ttggggcgtg actcaaaaag gctattacat tccagaaatc
    26881 gcttctcaat tctttgtgat gggattagtt gctggaatca ttgctgttct tttcaaatta
    26941 gatggaatga aagtcaatga catcgcttct tctttccaat ctggtgcagc tgatttagct
    27001 ggtacagcaa tcgtagtagg tatggccaaa gggattcttc ttgtcttggg aggatctgat
    27061 gcatctgttc cttcagcttt gaatacgatt ttgcatggga tcgggacggc gttgaccggt
    27121 gttccagctg tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt caacttggtt
    27181 gttacatcaa attcaggaca agcagctttg actatgccaa tcatggctcc actggcagat
    27241 ttagtaggtg tatccagaca agtagcagtt cttgcttatc agcttggttc aggttttatg
    27301 gacgctttta cacccgtatc agccagcttg attggtgtgc taggtgttgc acggatcgaa
    27361 tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt tttaggaact
    27421 attttcatca tgattgccat tgcaataggt ctgcagtagt actggagacc tgtaaataaa
    27481 aaagtaatcc aaacagtaga taaggactcc aagaaccgtc attttacggt aaaatcaaag
    27541 gagtaaagat cctatcgttt ggattatttt ttaagatcct atcatcagtt atacttaaag
    27601 aaagaggaaa aatgtatgaa aatcattcga caaattgaag tgtttgcacc agattactta
    27661 ggaaaaatgg atgtactgat tgccggtggg aagattttgg ctgtagaaga acagctagaa
    27721 ggcggctacg aaggagtgga agtagaagaa ctttcaggtg agggaaaagt actaaccccg
    27781 ggtttcattg actgtcattt ccatatttta ggtggaggag gagaaggcgg ttatcaaaat
    27841 cgaacgccag aagtcacatt gagccaattg actacagctg gtgtaacgac agttgtcggt
    27901 tgtttgggaa cagacggaga aggtagagat atgactgcgt tgatcagcaa agcgaaaggt
    27961 ctggaagcag aaggaatctc cacttatgta tacgaaggtt cgtaccgatt gccggtaaaa
    28021 acagtaacag attctatcat caaagacttt ttgacaattg ataaaattat cggaatcgga
    28081 gaaatcgcag tatccgatca ccgttcttct cagccaagtt ttgaagagtt cgctcgtgca
    28141 gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat caatgtccac
    28201 ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga aacagaaatt
    28261 ccgatcacac aatttttgcc tactcatgca aatcgcacac cagaattatt tgaagcttgc
    28321 gtggcatttg ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga tcctgatttt
    28381 tgggagaaga cagatggtga agttcgcttc agcaaagcac tgaaacgatt gatcgaggaa
    28441 gatgtttctt tagataattt cacgatgacc tcagatggac aaggaagctt gccttatttt
    28501 gatgaaaaca atcatttcct agggttagga gtaggaagtg ccaaggcatt gctagtaggg
    28561 atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg agctataacc
    28621 agtaatccag cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat tggtgctgat
    28681 gcagatctgt gtatcttgga taaagaaacg cttgatattg atacagttat cgctaaagga
    28741 gaaataatgg tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa aa
//
LOCUS       DACDNO010000037        25895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDNO010000037 DACDNO010000000
VERSION     DACDNO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.037"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(179..586)
                     /locus_tag="IWI22_001643"
     CDS             complement(179..586)
                     /locus_tag="IWI22_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9169036.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(775..1602)
                     /locus_tag="IWI22_001644"
     CDS             complement(775..1602)
                     /locus_tag="IWI22_001644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP9169037.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            1911..2129
                     /locus_tag="IWI22_001645"
     CDS             1911..2129
                     /locus_tag="IWI22_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9169038.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2150..2785
                     /locus_tag="IWI22_001646"
     CDS             2150..2785
                     /locus_tag="IWI22_001646"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9169039.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2978..3217)
                     /locus_tag="IWI22_001647"
     CDS             complement(2978..3217)
                     /locus_tag="IWI22_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169040.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3241..4764)
                     /locus_tag="IWI22_001648"
     CDS             complement(3241..4764)
                     /locus_tag="IWI22_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9169041.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4782..4904)
                     /locus_tag="IWI22_001649"
     CDS             complement(4782..4904)
                     /locus_tag="IWI22_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9169042.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4934..5917)
                     /locus_tag="IWI22_001650"
     CDS             complement(4934..5917)
                     /locus_tag="IWI22_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9169043.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IWI22_001651"
     CDS             complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IWI22_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9169044.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6111..6488)
                     /locus_tag="IWI22_001652"
     CDS             complement(6111..6488)
                     /locus_tag="IWI22_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169045.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IWI22_001653"
     CDS             complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IWI22_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9169046.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IWI22_001654"
     CDS             complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IWI22_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9169047.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7506..9278)
                     /locus_tag="IWI22_001655"
     CDS             complement(7506..9278)
                     /locus_tag="IWI22_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169048.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9232..10986)
                     /locus_tag="IWI22_001656"
     CDS             complement(9232..10986)
                     /locus_tag="IWI22_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169049.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11096..11665)
                     /locus_tag="IWI22_001657"
     CDS             complement(11096..11665)
                     /locus_tag="IWI22_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9169050.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(11683..13107)
                     /locus_tag="IWI22_001658"
     CDS             complement(11683..13107)
                     /locus_tag="IWI22_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9169051.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(13109..13777)
                     /locus_tag="IWI22_001659"
     CDS             complement(13109..13777)
                     /locus_tag="IWI22_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9169052.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(13908..14492)
                     /locus_tag="IWI22_001660"
     CDS             complement(13908..14492)
                     /locus_tag="IWI22_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9169053.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(14823..15056)
                     /locus_tag="IWI22_001661"
     CDS             complement(14823..15056)
                     /locus_tag="IWI22_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9169054.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(15071..16021)
                     /locus_tag="IWI22_001662"
     CDS             complement(15071..16021)
                     /locus_tag="IWI22_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9169055.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16018..16860)
                     /locus_tag="IWI22_001663"
     CDS             complement(16018..16860)
                     /locus_tag="IWI22_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9169056.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(16993..17718)
                     /locus_tag="IWI22_001664"
     CDS             complement(16993..17718)
                     /locus_tag="IWI22_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9169057.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIVHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(18179..18493)
                     /locus_tag="IWI22_001665"
     CDS             complement(18179..18493)
                     /locus_tag="IWI22_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9169058.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(18509..20104)
                     /locus_tag="IWI22_001666"
     CDS             complement(18509..20104)
                     /locus_tag="IWI22_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP9169059.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(20127..21017)
                     /gene="iolE"
                     /locus_tag="IWI22_001667"
     CDS             complement(20127..21017)
                     /gene="iolE"
                     /locus_tag="IWI22_001667"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP9169060.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(21034..22059)
                     /gene="iolG"
                     /locus_tag="IWI22_001668"
     CDS             complement(21034..22059)
                     /gene="iolG"
                     /locus_tag="IWI22_001668"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAP9169061.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(22083..23090)
                     /locus_tag="IWI22_001669"
     CDS             complement(22083..23090)
                     /locus_tag="IWI22_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9169062.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(23203..24363)
                     /locus_tag="IWI22_001670"
     CDS             complement(23203..24363)
                     /locus_tag="IWI22_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP9169063.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(24439..25893)
                     /locus_tag="IWI22_001671"
     CDS             complement(24439..25893)
                     /locus_tag="IWI22_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP9169064.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         7945 a         5296 c         3974 g         8680 t
ORIGIN      
        1 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
       61 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
      121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
      181 agtaaatgta gtaatctttg ctttcttttt tctcaatact aataactaac ttatggggta
      241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
      301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
      361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
      421 tttccttgcc atcaatttga acgatggcat atttgatagc atcttcagtt gtttcattct
      481 ccttttctat agagaataca aataagggaa taaaagaggg aataatcaaa atgccaataa
      541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
      601 taaacatatt agaatttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
      661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
      721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
      781 cgggtttttt tgtataatca tttgacactc aattttaggt aaactagcta cccaataatt
      841 taatgattca atacttttat ggctacccga tgtttcaaag atcgtcgaaa aatcactatg
      901 ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat ttttatccgt
      961 aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa aacttgagat
     1021 accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact ctttgaacca
     1081 gcccattgaa taggaagtct catctatatt aaaaagattt gtatttagaa ttttcgataa
     1141 gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact tagcaattag
     1201 accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta aaagtaatat
     1261 gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt cttcaactac
     1321 attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat cccaaaaacg
     1381 ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca aatcaatgtc
     1441 taaagggttt ttagctaata taattgcttg aataattgga attggtttgc tgattcctga
     1501 agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta gttcaacaat
     1561 caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa gtttttctcc
     1621 agttctttta aaacctttct tgtcgactaa taccccatca acatttactt cccctatgtc
     1681 atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat ttagaagtat
     1741 cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt catagggaac
     1801 ctctttctat accttgaatt tacactttaa gtgcaacaca taaaaatgag tcataatcga
     1861 tatccaacta gtataagttt gtcgagaaca ctcctagaag gaaaataatt atgagccagt
     1921 attgtcatct tactatactc aaacgagaaa gaatctttct gcttcacgaa gaaggatttt
     1981 ctattagtat aattggcaac gacattcagc atagcccgtt tactatttta catgaactga
     2041 gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa aaaaattatg cttatcgaag
     2101 aaaaaaatgc ggacgttatc acattctaaa tgatccgtga gtttggtcaa ttgttcgtca
     2161 tttatttgtc gattttcagt ggtcgccaga gaaaatctcg aatcgtttga agcatgaaca
     2221 tatggacttt tcaattagtt ttatcacgat ctaccgtgca atttgcaatg atcttttcga
     2281 tacagaaccc ctctcacacg gcaataaagg cctgatccgt tcattgcacc atcgaggaaa
     2341 aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag atttgcctta ctcattcgat
     2401 ccatggccat ccaaaatccg cagatgatag aagtgatttt ggtcattgga aagccgatat
     2461 tgtagctggc aaaactagtg gtacttgttt agtgactttg acagacagaa aatcatgctt
     2521 ccttttagca ggaaagaagg tccccagaaa gaaagccgct tatgtgagcg agaaaatgat
     2581 tgaactttta agcagtattc caaatgaggc ggtcaaaacg atcactccag atagaggaaa
     2641 agaattctcc aatcatttcg cagttacaga agtattgaat aatatgccct tttattttcc
     2701 agatcctcat gcccttggca acaatgaaca aattaaaata cgaatagact cattcgagaa
     2761 tacttgccaa aatcgaaaga gatagctacg gtagataact caacgataaa tcagatgatt
     2821 gaaaaactga ataacagacc tagaaagtgt ttagattgga gaacacctta tgaagtcttc
     2881 tttgataaag tgttgcgctt gatttgacaa ttcaagaaat aataaaaagc agatcaaatc
     2941 caaataaggg aaaaatgatc tactctggta ttatcaatta attggccaag ctattatgcc
     3001 gttttgctaa acgtctactt cttccagtat tgaccttgat aaaacaacaa tcaggatccc
     3061 gttttaatac tctatgaact gtgtcaatac taacatgtaa agttgcagca atatcagcta
     3121 atgtactccc atttaatctc atttgctttg caaattgata acgccgataa gcatcaatat
     3181 tcttggaatc catagacaat aatacttttc ttaccatgta tttcctccaa agattggatt
     3241 ttaatgggcc atcatttctt gagcaatctc atgctcagat aatgaagctg gatagaaagt
     3301 aggccaatta tgcatttcat tataaagttt ttcttgactt tctccaccaa agaaaatatg
     3361 gaaatgctct gccttacttg gtgcaattcc atgatcgcta aattgaatat atttaaaacg
     3421 accagcacct gcatcatcag tttcaaaatt aaaacgaaca ccacggtttc ctttttcata
     3481 attcaaaatt ttgtatccaa catatttgta tgtgtattga tgatgttcac cgttgactac
     3541 aaaatcaatg gtgttgtctt taatgttgat agtatcaata tctgtcttat agccagtagt
     3601 ataataatct ttgtactcgg cagctgtcat gtctttattg agttttgctt tgtaatcgaa
     3661 aacttcatcc aaaattccat cttttaacaa cggataaaca gattgccatt cgcctgtata
     3721 atcactcaat tcgcgatcct tcacatcggc atctttaaag tacccattag caactgtctt
     3781 ttcatccttc gcttcttttt ctggaactac ctcttcacca gcaaccatag ttgttttctc
     3841 taacgctttt agattgtctt ccattactga aacataatct tcaccattat ccatttgttc
     3901 ttttgtaaga ctttcaagag gattcaatac ttccaatttt atcccagctt cattggccaa
     3961 agtttttgcg attttatcat tagcattctt ttcaaagtaa atataattga tcttattttt
     4021 tttaacatac tcttttaatt cagctaatcg accagaagat ggctcttctt caggacttag
     4081 tccagcaata ggaacttgta tcaatccata atccaaagct aaatatccaa aggctgcatg
     4141 ttgtgtcaca aaactttttt gtttcgcttc tttaagacta gtggtatagt cagcatccaa
     4201 ccgtttcagt ttagtcaaat acttttcagc atttgtctca aatacttttg cttttttcgg
     4261 atacaattta actaactgat ccttgatatt tgaaacttcc ttaattgcca ttttgggtga
     4321 aacccacgta tgaggatcta gttcatgatg gtgatcttct tctccgtgat catgatcttc
     4381 ttcttcacta cctggcaata aaatcatgtc ttttgttcct tctactacat taggcccagc
     4441 tttcttccaa ctttctttag cttttggtac ccatgattcc atgttttcgt tatgatatac
     4501 aaaaacatca gcatcatgag cttttgccat atccttagct gagggctcat agtcatgagg
     4561 ttctgatccc gctggaacca ttaaatccac gttcgcttca tcgccgacga tgtttttggt
     4621 gaaatcatac attggataga atgttgttac aatttctaat tttccatctt tatcattcga
     4681 tacttttgca tcattagtgc tggagcaagc tcccaataat cctagagctg ctatcccaat
     4741 aagtcctaca ctgattttct tcattctttt tccttcctta attatgctaa tttcttcatt
     4801 ttattttgtt tatgttcttt gataattttt aatgcccttt ttctagtttt gatattcggg
     4861 cttttaagtc gtcgatatgc actagatata tccgttttct ccattagaaa ttacccctaa
     4921 tcaatactta tcattatttg ctttccgcca gtaatttatc aaaactctgt ttgatccttt
     4981 cttcatccaa catttttcca attagaataa tagtggtttg gtctttttgt cgagtggtgt
     5041 attgaaaatt aacctgttcg tttacccctt gtatggcaac tataaaatct tgatcttgca
     5101 aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt ggttaaaatc agccggtcaa
     5161 ttaattggaa taaaaaatct ttttttatgt cttgatctga agttaagaga attgatttaa
     5221 actcatgttc ttgatgatgt tgatgatagt gtttgccatt atagtgattt tctgatgaat
     5281 cactttcaaa aactaaggga tgattgaatt tttccaaatt ataaaaatcc tttttttgaa
     5341 tcgtttcact atgtgtgaac attcgaatat cggataaagg gttaatactt ttgatttctc
     5401 tctgtaattt ttttagtgca gataaattca agttttgttt tctagacaca aaaatgcgat
     5461 ctgctatgac aagttgctta agggcttgtg gttctctttc aataagttca aaactgtcac
     5521 tatcaataac agtcaataaa ctatcgatat atactcttcc tcttagttga ggcgctgtta
     5581 aaagtgtttg aatgattggt tggggatctg ctatacctgt agtttcaatg atgatttgtg
     5641 aaattggttg ttgatgttct tctattgttt ccagtaaaga aatcaatatt gctaataaat
     5701 cactgcgaag actgcaacac atacatccat tatttagttg taacacccgt tcctcgctat
     5761 gaactaataa tttgtgatca atcccaactt caccaaactc attttcaatt ataataattt
     5821 cttccctggg gtagggtgag acttgcaagg cttgattgat tagcgttgtt tttccagccc
     5881 ctaaaaaccc agaaacgatt gtaactggta tgcccatttt gtcacctttt taccacttag
     5941 ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt ggcgaatact tttttaattc
     6001 taaccgttca gggttattcc gtttattttt gctggtgaga tacagacgtt ctccagtttc
     6061 gacacattcc aaaataatat tttgacgcat aaatcttcct ccttcaatac ttatcgatct
     6121 tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta actcgccaga ttccaattgt
     6181 gaaactaaat tttctttcat atcaacaggt atctcccaat aaccatcaat acattgaaat
     6241 aactcctcgt catactcttc aaaaattgga tagattctta aaaaatcatc aaatgataag
     6301 ccttggtcct catagtaatc tttgactaat ccattttcaa ttgcctgttg ggtcggtaaa
     6361 cccgtttcag taataattcc tctctctacc atcgaacgat aaaaagtgca tttatctagc
     6421 gattgctggg tcgtttcaag ataatacttt aatccttctt caactaaagg tgggtaaata
     6481 atttccattt tgattccctc ctaaaagtta tatattaaac tattttgaag ctcctttaat
     6541 tactttcttt ccattatagt acccttttaa agatacatga tggctacgtc tataatcacc
     6601 attcgcttcg tcaaatgaaa tttctggttt tactacagtt tgatgagttc ttcttaaccg
     6661 tttcttcgcc ttagatgttt ttcttgctgg tactgccatg atatttcctc cttttaccaa
     6721 cttgcttttt ttactccagg aatcaaccct tgatgggcaa gttctctaaa cttaatacgg
     6781 gacataccga actttcgcat ataccctctt ggacgaccat ccgtctgatc acgaagcctc
     6841 agacgattcg gatttgaatc tcttggcaat ttagcgagag cggtgcgatc tcctgcggct
     6901 tttaattcct gacgtttatc tgcgtacttt tcaatcatcg ccatttgttt ctttgcttta
     6961 gcaattttcg acttttttgc catatacctt tatactcctt ttattttgat ttagacatct
     7021 caaatcgtaa tcgttacgtt ttgacactga tactttacca acaagagttg ctcttgtcaa
     7081 caataaataa catgaaataa aactcatatg aatagataac tatttgaaaa cgaatttatc
     7141 ggtacataga gataatcaaa tcatttaaaa gaatatgcag tttaagctct gaaaaataag
     7201 aattcacttt tattagtcca atcaaacaca aataccgttt taagtagctt ttcctctact
     7261 taaaacggca atatgaaaga aactataact ttttattcaa tcttttatgt ttaactggtt
     7321 tatcgccaat tttttaaaac tttgattatt ccttatatta taagaataga cattaagtga
     7381 actaattcaa tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt
     7441 attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta
     7501 tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc
     7561 tttatgagat ttttgtatat ttctgacctt cgatacaact cactatgagt accttgatca
     7621 actatttttc cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga
     7681 tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt
     7741 ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt
     7801 ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg
     7861 ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta
     7921 gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata
     7981 ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta
     8041 aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat acagccttca
     8101 tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga accactagca
     8161 cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg
     8221 gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga
     8281 ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga
     8341 gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt
     8401 attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag
     8461 tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc
     8521 atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt
     8581 ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat
     8641 tctatagttt cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg
     8701 gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa
     8761 ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa
     8821 aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca
     8881 acgtcagaca tgatagtttg agataaatct gataaattat gttttgaaaa ataggataat
     8941 ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat gcgcactttc tttataagta
     9001 ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaacg
     9061 aacgatactg taaaataaag ccaaggcggc tttacatttt ctaaaactaa ttcgtctatc
     9121 agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa gctagaaata
     9181 gatgctttaa ccaaatcttc tgcgccctga ttagttaaag cgaatttgtt tttataaaaa
     9241 cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata
     9301 cacttgatgt ttagccatta attcattatg atttccacgt tctactattt tcccttctga
     9361 caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta tcataataac
     9421 tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc
     9481 aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc
     9541 aatagctata cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt
     9601 atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc
     9661 aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg
     9721 attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga
     9781 atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc
     9841 tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat
     9901 tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa
     9961 gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag
    10021 ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc
    10081 aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa
    10141 aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa
    10201 aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac
    10261 aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc
    10321 tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc
    10381 tcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat
    10441 aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat
    10501 tagaaccaat ccaattttaa aattaatgaa aaagcctaag gcaactgata aaacaggtgt
    10561 tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc
    10621 gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact
    10681 tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag
    10741 gatatgagaa aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac
    10801 agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta gagacttaag
    10861 aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa
    10921 agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac
    10981 tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc
    11041 tttccgtaat agcgtaaatt agagatgtac aaacagatag aaggtaaact acgttctatt
    11101 ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta
    11161 caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg
    11221 ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata
    11281 taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag
    11341 gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc gccatactaa
    11401 tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa
    11461 caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata
    11521 tgattccaga taataatcca aaaatccctg gggatatgct gtgaccaaac gccccaccaa
    11581 tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa
    11641 taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt
    11701 aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt tttttattcc
    11761 accatttttt aaataaataa ttctattgca tgttttataa agaaattccg gatcatgagt
    11821 ggctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa tcctttccat
    11881 attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag tttttgacaa
    11941 tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa acggtaattg
    12001 atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat ctttactaac
    12061 tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt ggtaatccat
    12121 ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta tctttccttt
    12181 ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca caccgttttt
    12241 ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt ttagaatatt
    12301 ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga tatagggaac
    12361 ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa
    12421 caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa
    12481 ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa
    12541 atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc
    12601 atccaatata attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg
    12661 ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc
    12721 agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga
    12781 aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac
    12841 gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa
    12901 taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt
    12961 taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac tacctttttt
    13021 tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac
    13081 attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa
    13141 cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt
    13201 tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag
    13261 ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag
    13321 acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag
    13381 tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt
    13441 tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac catgagatca
    13501 ttacgaccgg caacattcga aatacaatat ataaggatgt cccaaaaaat atagtaaaaa
    13561 actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga
    13621 tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga
    13681 aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat
    13741 ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga
    13801 tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta
    13861 tcaaaaagca taactgagaa caattctcaa tgcaagcttt tttttaatta actcagtaat
    13921 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
    13981 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
    14041 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
    14101 tctagacaac agactgtctt ttttgtcaca aatgatgtaa ttttttcaac aatatcttga
    14161 tatttggttc cagctgattg gaataaaacc aaatcgaata tacgtttatt ctcaaaaata
    14221 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
    14281 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
    14341 atatgcggct gaataatttc ttcaaatagc gcttccttag aagtaaaatg tttataaaaa
    14401 gcacccgtag ttacgcctgc ggtcaagcac agttgcctta tcttagtttg ctgaaatcct
    14461 actttttcaa aatggcttag tccactttct attaattttt ggtgagttgt aatataatct
    14521 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctatattatc ttacacagga
    14581 cttgaagttt caatcgttaa cctctattac tgaactcatt ccttagagct aaactaatga
    14641 gaaagagcaa aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
    14701 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
    14761 gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagcc
    14821 ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga
    14881 aatcctatat ctaaatagtc ctcatatcta agtttagtca gcatctcact tacagatgct
    14941 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
    15001 aattcctgaa ttgctagtaa ataaattagt atagtagttg ttttaatttg taccatacct
    15061 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
    15121 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
    15181 agataggaat attggtatct tttgatactg ttttcattgg tctatcgtcc acactgcttt
    15241 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
    15301 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
    15361 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
    15421 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
    15481 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
    15541 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgggga tcttccttcc
    15601 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
    15661 ctttgttttc ctctttgttc gcatttttta ctaattttgt aaaccaagca tttcctccag
    15721 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
    15781 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
    15841 ttttatcttt tgctatattt tcagtaatat ctgctaaaat cgaattagta actactacct
    15901 ttaatttttc gttcgaagcc actgtatctt tgtctttact atttgttgaa catgccccga
    15961 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
    16021 taactttctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
    16081 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
    16141 aatgaacaaa cctagcaatg atgagcacgc tccgactgtc gccgaaataa atatcatagt
    16201 ttttaaactg tttgcatata aatatgctgt agcagctggt gtaattaaca ttgcaacaat
    16261 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaatcattaa
    16321 tagataatga taaaaactga cattcatccc cattgctttt gccattaatg gatcaaaaga
    16381 agtgattaat aattctttaa aaaatagaac aactgtaacc aacactaaaa cagaaacgcc
    16441 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata agatatgaaa
    16501 taaatccgtt gagctatttg caactcctat aaggataact cctaatgcta aaaaagaact
    16561 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
    16621 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
    16681 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
    16741 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
    16801 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
    16861 attattcccc ttgtatatta gagttagggt tttaaccttc cggttttgat tggataaccc
    16921 tatgaaccat cgttatttta atttggtatt aactcatttc ttttgagata atatatttat
    16981 tggattgaga ggctaagccg ttcccccctc tccaataaat atagtatctc cataggcttt
    17041 ttttaaattc tcttcattaa atacagattc tacacttccg tgaacaatta gatttcgatt
    17101 caaaagaata atattatcaa aatacttctt taccttgctt aaatcatgat gcacaattag
    17161 cactgttttt ccctgatgtt tcaattcttt taacgtatcc ataattattc gttcactaac
    17221 taagtctatc cctacaaatg gttcatctaa aaatatgaaa tccgcatttt gagcaagaca
    17281 tcttgctaaa agtacacgtt gaaattgtcc tccagataat tctcctattt gatgattaga
    17341 atattttccc atatcgacct tttccaaagc ctttgaaact ctttgccatt ctgcattttt
    17401 aattctttga aaaactttca ttccggcata tgttcccata gatacacatt cttttacttt
    17461 tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct tcaccagttt
    17521 tttattgata tcttcattat taatggagac ctctccctga tgagggataa tatttaacat
    17581 tgctttaagc aatgtagatt ttccagcacc atttggacca ataatacctg ttaaagcacc
    17641 ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac aaacattaag
    17701 ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca cgatcatatg
    17761 gtatacaaaa attatatttt agtcaaccct aaattttata atttagattt ttttctgttg
    17821 ttgaagatta ggatgtcaaa attatttgaa actactcgta tagtaaatag taagtaattt
    17881 tcaaaacacg aagaaaaaca aaaagaaaaa aggaaacgcc gaagcatttc ctttcagtga
    17941 tttaatgaga acaacacgat ttgacttaga gtctcaaaaa gtcgaatagg tacactcatt
    18001 taagtgtctc cattcgactt tttggggatg agctggctat tgaggtccgc ttaccctatt
    18061 gaataaatac aagattgggt atttctaata agtccgccct tcatattggg gtgatcgggg
    18121 cgaacatgcc atcttttcat ataccacctc ctcgacactc aataatcaaa gattctcgtt
    18181 aatcttcttc gatgcttgca atgatgtttt caatattcat ataacgaaaa tcttcttgtt
    18241 tgatataaac gatctgagcg gtacattttt cctttttgat gatttctttt actttttcct
    18301 cagaacgtgc gacctgcggc gttaaaagta aaacgtcata gtctttataa ttcagttttt
    18361 cttcaacgtt tcggatcgtt gcataatcta tctcatacga tagatttttg gctctgatcg
    18421 tttcaagcat ttttgatacg agcagcgacg cactgattcc ggtctcgcaa ataatcaata
    18481 gatccttatg cataataccc tcctgttttt agcctgcaaa gccaattggt gaaaatgcca
    18541 cataagttaa aataacaatg actaccaatg ctatagcagt caccttggtg tatttccaag
    18601 gctcgatatc gacctttgta tcgaatgagt tcaacacaaa ttctccatca ggtttcaatt
    18661 tggaaaccac catcaaaatg ataatgtcca gaacgaataa tgccgtcaaa acataaagga
    18721 aatggatatt ttggaaaagc atttttgata gtgcatacaa gacgatatgc acggcaatcg
    18781 tcgtttttgc cccaaaagca gttgcttttt ttgaataaaa ggcaaagaga atgatcacga
    18841 gcagcggcat gttgtatagt ccattgaatt cttgaacgaa ctgatacaat ccagccggtg
    18901 caaaagaaat gaatggcgct acgataatgg agataatccc cacaacgacc gttactgttt
    18961 taccagcacg agcgatttgc ttatcatttg ctttcttatt gataagcggt ttataaaaat
    19021 ccagtgaaaa caaggtcgat gtcgcgttca aagctcccgc aaatgatgat aagatcgcgc
    19081 caaagataac agcggcgaaa ataccaaaga tcattttagg caatacttca ctgactaaaa
    19141 aaggatacgc gttatctgga tttgacatta aactatcgcc aaaaatattg cgagcgacga
    19201 tccctggaaa gacaaggaat aacgcaccta agattttgaa gacgccgact aggaaagcgc
    19261 ccttttgtgc ctcagctaaa ttcttgccgg acaatgcttt ttggacgatc atttgattgg
    19321 tacaccagaa atacagattg ttaaacagca tccctgtaaa tagtgtcggc catggaacaa
    19381 cagccgagtc gatcgatccc acagagttaa gcagccatgg cgtgtttacc acaacgtgtt
    19441 cgatcccgcc aacaaagctg ccgtcaccaa gcttcattaa gcctaaaatt gggaccagca
    19501 gaccacaagc cagcagaccg actccataaa tggagtcact attggcactt aacgacaagc
    19561 cgccgatcag cagatagagg atgccgacaa taccaattgc catcgctaca attatgatcg
    19621 cgacgatcgg ctggacgccc agcagctcat caatatggaa gatcttattg aacaccagcg
    19681 atccagaata aagaaccacg ggtaagaaag aagtcatata ggtcacaata aataagatcg
    19741 aaataattcg tttggtaacc gtatcataac gaatctcgat gaaatctgag attgtattga
    19801 tgccgtattt aaaatatttt ggcaagaata tcacagccaa agctacgatc gcaatcgtag
    19861 cagttacttc ccaggccatt acttccatcc ctgcatgata actttgacca ttttgtccga
    19921 ctatttgttc ggtggacagg ttcgtcatga tgatcgtgcc ggcgattggc aaagcagtta
    19981 agctccgccc tcccatgaag aacccttctg atccagaagt atcgacactt ctggatctag
    20041 aataggcaaa taaccataca cagatgacaa tcactaggaa actgattata gaaaaaagat
    20101 tcatcgtcta gctccttttc aatagattaa agaccggtga cctctcgaat gtagcttctg
    20161 gctttgactg catattcaaa tggattggct ttagccgggt cctgttctgc ttcaactacg
    20221 atccagcctt catagtcgct ggcatggatt gctgtccaga ttggagtgaa atcaatcatc
    20281 ccatcaccag gaaccgtgaa gacgcctttc ttcacgccgt ttaagaagct gtctttttcc
    20341 ttttctactt cttcagccgt ttcttgacga atatctttga aatggatgtg tttgatacga
    20401 tcttgatgac ggttgtaaac cgcgattgga ttttcaccag aaaagaccaa atgtcccgta
    20461 tcgaacaata atttaacttt gtctggatca gtcatttcca ttaaacgatc gatttcttcc
    20521 gtcgtttgaa cgccggtacc catgtgatga tggtaaacga tctccatatc cttttcgtgg
    20581 gccaaatccc ctaatttttc taatccttcc gccagttttt tccactcttc atcagtgaaa
    20641 actggtttat catcaaagac tggcgctgtc ataatccctt ggatactatg accctgctca
    20701 gctactacga tgactttagc gcccatcgca tgtaagaaat cccgatgtgc tttgaaggct
    20761 tcggcagttt cttcaaaagg tttagttgtc agaaacgcac tgaaccatgc actggcaacc
    20821 tttaagccgc gaatattcaa atattgttcc aacacctctg gatcttttgg atatttgttg
    20881 ccaatctctg ttccggtgaa cccagcaagg gccatttcac tgatacattg ttcaaaggta
    20941 ttttcactac caagctcggg catatcatca ttggtccacg cgattggtgc gatggctaat
    21001 tgaatttgtt tggtcatatt ctcacactcc tgcttatttt tttagttgta ctaaactctt
    21061 cgtatcgaat gattcttgac aagccaacgc gatcttagtt gattgcaatc catcttctgc
    21121 attaaccgct ggttgacgtt tttcttgaat acatgagaca aattcttggg cttcacgaat
    21181 gaaggcttct ctgaatcgtt ctgggaagtt ttgtgaacat ggacggatga tcccttgatc
    21241 gttcatgatc gtcacaagat ttttctcagg cgcttgcgct acccgcagca ttcctttggt
    21301 tcccatgatc tctgtttcga catgatagcc atgggctgcg tttctgccgg caactagtaa
    21361 agctattgtt ttatcgctca ggctcatcat tgcggctcca gtttcaagct caccagcctg
    21421 atccaattct ggatacgccg catttttccc aatcgcccac acttgatcga cttcttggcc
    21481 ggtaaaccag cgaaccaaat caatatcatg gatcgccata tctaagaaca agccgccgct
    21541 gtcgttattc atggcaaatt tgacaaagct ttccaagcct tcgctcggat cgatcccata
    21601 acaacgaatc agtgtgattt cgccgatttc gccatcttct accattttct tcgcatactg
    21661 atacgactca tcatagcggc gcatgaagcc taacatgaag acctgttcag gatgctcttc
    21721 gatgactttt tgggtctttt taatatcttc aacttcaagg cctaatggtt tttcagtaaa
    21781 gacatgcttg ccattttcca tcgccaagcg gacttggtca cagtgaaagc ctgatggtga
    21841 aacaataaag actgcatcga tcacggaact tttgaccatt tcttcatagc tggaaaaagt
    21901 atgcttgacg cctaatttct tctgtgcgaa tgctaattct gtttcaacta aactgcacgc
    21961 agccgtaagc tcacaacaac tgacacctgt tgctaaattt tgtgcatgta cttttcctaa
    22021 tcttcctaaa ccgacgatgc ctactctaat gttgttcatg aaatacctcc gttttctgag
    22081 aattactcat tgtatagtgc tggtcgttca tccatttgaa tcattacttt tgtttttgtt
    22141 tcacgagact catgacaagc catcatggtg atcgctgcta catacccatc ccaagcggta
    22201 ggaccgacaa ccgcatcatt tttgatcgaa tcgatccaca gcttgaattc ataatcatag
    22261 gcatccaaaa agcgggtctg ccaatctggt gaaacaggtg tgtaattttt ttcttccgat
    22321 ttaattcgtg tataggctgg gtccgtcatt cctacttctg ccgtttcgaa aaccacatca
    22381 catttaatat cgtagccaaa gcggcaattc acaaagattt ccaaatcaat cgttacaccg
    22441 gattttgttt gaaagatcag cagctgcgga tcatgcaaat cggcatggga gttgctggtt
    22501 tttttcggca agatcatttg aacagattca taatcttcat ttagcaacca gcgaagcaca
    22561 tcaatctcat gaatcgcggt gttgctgatc gccatcggtg ttgtgtagtt ttcatctgcg
    22621 ctaggatttc tatgggcaca atgcatcatt aatgcttctc cgtattgctt cgattcgatt
    22681 gcttctttca gttcaatgta gccgcggtca tagcggcgca tgaagccaac ctgaaccaat
    22741 ttcttcccat aagcaacttc cgcatcaacg atccggcggc agcctgctgc atccatcgct
    22801 aatggctttt cgcagaaaac atatttccca tgcttgattg cttctaacac atactgctca
    22861 tgggtcgggt cccaagaagt cacaacaacg acatccacat cttcagaagc gatcaatgct
    22921 tctccggttt caaagaattt agcatcgaca cgtttggcaa cctctttagc agcttttaca
    22981 ttgatatctg agactgctgt cacataagcc ccttgtgttt tctgattgat cctgtcgata
    23041 tgctcgatgc cgattgcacc tgttccgata acgcctactt ttactctcaa ttacttatcc
    23101 ccctgttctg ttgtttaagc acacatagta tgcgcttaca aaattattcc tgtgtagctt
    23161 ttattcttaa aaggtagagc cctctctttg ctccgccttt tactacccat aagcttgtac
    23221 gtataattcc ttaatttgac tatgactagg caccttttta ttattttgcg gacttccgct
    23281 ggtcaacgca tccgcggcca ttttgtctaa ggctgccata aacgcttgct gatcaatacc
    23341 ataatctcga atcgttggga tcgacagctg ctcacagagc tcttggattt ttttaatcag
    23401 ataatccgca gcctcttcca gcgataaatc ttcggctgcc ggataaataa atctggcgat
    23461 cgtcgctaac tcagtctgaa tagcatcctt tgagaattca aaaaagaccg gcaataacat
    23521 cgcatttgat atcccatgag gaatgtgaaa aagtgccccc accggtcgac tcattccatg
    23581 aatcaatgtg actgacgcat tcgaaaaggc aatccccgcc tccgttgaag ctaagctcat
    23641 ttgttctctg gcttcagtgt cgtcgctatt ttcatatgcc tgagaaagcg tgttcaaaat
    23701 tttcttcatc gcttccagtg aaaatagttg cgttacttca ttggctttga ctgaaacata
    23761 agattccaaa gcgtgacata gcgcatcgac tcccgttgcc gcggttactt ttggtggtac
    23821 agttttcgta aaatcagagt caacgattga gatctgtggc atgaaaacga gatccttcat
    23881 catcattttg actgctgttt ttgtattggt aataactgcc acatcggtta cttccgaacc
    23941 agttccagca gttgttgaca ctgcaatcag cggaatgccc gcttgcagct gatcaaaatt
    24001 tcttgcatga atcgcttcga gacttaattg attagcagcc aaaacggcta ccaccttggc
    24061 tgtatccaga cagctgccgc caccaaaagc aatgatatga tcacattgat gcttctgaaa
    24121 aagcttcagc gcatcataaa cattttcttg cgtcggttcg gcttgaatgt ctgtatactc
    24181 cgctaattga tcatctgttt ttcccaacag ctccttgatc ttttgaagat gcccggtctg
    24241 ttccataacc gtatcactaa tgattaacgg cttgttgcct agttgttctg attcttccat
    24301 aagaaattgg attgttccgc cgccaattct taaattggca ggcattttca gttgaatatt
    24361 cacagaaaaa acctctctct ggcttatagc ctataacaga atcctcgaag ctctttaaca
    24421 gagcttcgaa gcaaatcttt aaatctcact ttgcgcataa cgtgctgtaa caactttccg
    24481 atgggtataa aactcaacac tgtctttgcc attggcatgc aaatcaccaa agaaagagtg
    24541 cttccagcct gaaaaggcaa accaagccat cggtgcaggc accccaagat tgacccccag
    24601 catcccagca tcaacatttt ctctgaagta gcgaatcgct gatgcgttgt tggtatacag
    24661 acacgaacca ttcgcaaatt cagattcatt cgtcagctca acggctgcca tcaaatcttt
    24721 tacccgcacg attgatgcga ccggggcaaa aatctcatcc tgccagatct tcatatccat
    24781 cgtgacatta tcaaaaatcg ttggtccaac aaaatagcct ttttctggaa tgttttcgcg
    24841 accatctagt accagtttcg ctccttcatc gacccctttt tggatatagt caaaggtccg
    24901 ctgctgtgca tcttttcgaa tcactggtcc taaaaagacc ccttcgtcca agccattgcc
    24961 gatcttgatc gcttttgccg cagccttaaa tttttcaaca aactgatccg cgatttcctc
    25021 ttccaaaaca atgacggagg ctgccatgca gcgttcacca gcagaaccaa aagctccgcc
    25081 aataaggtcc ttgactgccc gatcaatatt ggcatctttc agcacaacaa catgattttt
    25141 cgctccagtc aacgcttgta cccgttttag attttttgtt ccttcacggt aaacgtattc
    25201 accgacattt tttgatccta caaagctgat gccttttacc agtggatctt gcagcaatgt
    25261 gttgacaaca tcgtgggcac catagacaac attaaaaaca ccgctaggaa agcctgcttt
    25321 ttcaaccagc tcaacaattt tctccatcaa caatggtgtt ttttctgaag gcttcagcac
    25381 caccgtattt cctgccgcca gagccatcgg gaagatccag aagggcacca tcattggaaa
    25441 attaaacggt gtgatccctc cgattactcc gattgggtaa cgataattcg tgatctcaat
    25501 atcagtcgcc gctgttgata aagaatctcc catcattaaa ttggtgattc ccgctgcgtg
    25561 ctccacattt tcgattcctc gttgaacttc tcccagtgac tcggacaatg atttcccatt
    25621 ttccagcgtg atcaaacgcg ccaattcact tttatgatct gttaaaagct gatgcagctt
    25681 gaacagataa cgtccgcgaa caggaacagg aacttttttc caagtttgaa acgcagcatc
    25741 ggcagcttta actgcttcgg ctgtctctgc tttgctagac aacggtacat tcgccagcgc
    25801 ttcttctgta gccggattca ttacttcttc aaactctgtt gagcgactag cgacccaacg
    25861 accatcaata taatttctta aaaatcttgt cattt
//
LOCUS       DACDNO010000038        25672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDNO010000038 DACDNO010000000
VERSION     DACDNO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.038"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            171..515
                     /locus_tag="IWI22_001672"
     CDS             171..515
                     /locus_tag="IWI22_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9169065.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="IWI22_001673"
     CDS             516..935
                     /locus_tag="IWI22_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9169066.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1027..1377)
                     /locus_tag="IWI22_001674"
     CDS             complement(1027..1377)
                     /locus_tag="IWI22_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169067.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="IWI22_001675"
     CDS             complement(1343..2674)
                     /locus_tag="IWI22_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9169068.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2930..3496
                     /locus_tag="IWI22_001676"
     CDS             2930..3496
                     /locus_tag="IWI22_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9169069.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3641..3728
                     /locus_tag="IWI22_001677"
     tRNA            3641..3728
                     /locus_tag="IWI22_001677"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3673..3675,aa:Ser,seq:cga)
     gene            4062..5036
                     /locus_tag="IWI22_001678"
     CDS             4062..5036
                     /locus_tag="IWI22_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9169070.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5361..7256
                     /gene="asnB"
                     /locus_tag="IWI22_001679"
     CDS             5361..7256
                     /gene="asnB"
                     /locus_tag="IWI22_001679"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9169071.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7451..7897)
                     /locus_tag="IWI22_001680"
     CDS             complement(7451..7897)
                     /locus_tag="IWI22_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9169072.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8125..8274
                     /gene="rpmG"
                     /locus_tag="IWI22_001681"
     CDS             8125..8274
                     /gene="rpmG"
                     /locus_tag="IWI22_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9169073.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8362..8880
                     /locus_tag="IWI22_001682"
     CDS             8362..8880
                     /locus_tag="IWI22_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9169074.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8957..9913)
                     /locus_tag="IWI22_001683"
     CDS             complement(8957..9913)
                     /locus_tag="IWI22_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9169075.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10079..10885
                     /locus_tag="IWI22_001684"
     CDS             10079..10885
                     /locus_tag="IWI22_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169076.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10882..11871
                     /locus_tag="IWI22_001685"
     CDS             10882..11871
                     /locus_tag="IWI22_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9169077.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11868..12872
                     /locus_tag="IWI22_001686"
     CDS             11868..12872
                     /locus_tag="IWI22_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9169078.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13004..13546
                     /locus_tag="IWI22_001687"
     CDS             13004..13546
                     /locus_tag="IWI22_001687"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9169079.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13566..14264
                     /locus_tag="IWI22_001688"
     CDS             13566..14264
                     /locus_tag="IWI22_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9169080.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14285..14497
                     /locus_tag="IWI22_001689"
     CDS             14285..14497
                     /locus_tag="IWI22_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9169081.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14497..15459
                     /locus_tag="IWI22_001690"
     CDS             14497..15459
                     /locus_tag="IWI22_001690"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9169082.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15477..15872
                     /locus_tag="IWI22_001691"
     CDS             15477..15872
                     /locus_tag="IWI22_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9169083.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16035..16400
                     /locus_tag="IWI22_001692"
     CDS             16035..16400
                     /locus_tag="IWI22_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9169084.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16397..18208
                     /locus_tag="IWI22_001693"
     CDS             16397..18208
                     /locus_tag="IWI22_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9169085.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18270..18437)
                     /locus_tag="IWI22_001694"
     CDS             complement(18270..18437)
                     /locus_tag="IWI22_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9169086.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18685..19674
                     /gene="galE"
                     /locus_tag="IWI22_001695"
     CDS             18685..19674
                     /gene="galE"
                     /locus_tag="IWI22_001695"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9169087.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19686..21170
                     /gene="galT"
                     /locus_tag="IWI22_001696"
     CDS             19686..21170
                     /gene="galT"
                     /locus_tag="IWI22_001696"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9169088.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21194..22174
                     /locus_tag="IWI22_001697"
     CDS             21194..22174
                     /locus_tag="IWI22_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9169089.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            22263..23087
                     /gene="tsaB"
                     /locus_tag="IWI22_001698"
     CDS             22263..23087
                     /gene="tsaB"
                     /locus_tag="IWI22_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9169090.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23071..23613
                     /gene="rimI"
                     /locus_tag="IWI22_001699"
     CDS             23071..23613
                     /gene="rimI"
                     /locus_tag="IWI22_001699"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9169091.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            23620..24084
                     /gene="rimI"
                     /locus_tag="IWI22_001700"
     CDS             23620..24084
                     /gene="rimI"
                     /locus_tag="IWI22_001700"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9169092.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            24081..25097
                     /gene="tsaD"
                     /locus_tag="IWI22_001701"
     CDS             24081..25097
                     /gene="tsaD"
                     /locus_tag="IWI22_001701"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9169093.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
BASE COUNT         8327 a         4400 c         5377 g         7568 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggcaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactataact tgaaatgaaa gatttggaca actatcattt aggtcaaaac taaaaatgta
     2761 agggaaccca ttgaatttaa ccaaattttt ttaattttgc aaaaaaaagt taaagaagac
     2821 tgaaaaaaaa tttaaaatat aacgttttga gtaaaatgtt tgcaaaaaat gctaaaaata
     2881 gggtacaata aaacaacata tactgcagta aagaagtggg ggagttttaa tgagaagaaa
     2941 agtctatacc aaggatcaaa ttctaaaagc tgcttatgat gttgttgcaa aggaagggtt
     3001 taaaggattt actgcgagaa atattgctaa aaagatgggg atttcgacac agccgattta
     3061 tctggaattc aaaaacatgg aagatttaaa aaatacgttg ctggattcta tctttgaaca
     3121 actaaaagaa gatgttttcc ctattgaacg tactggtgat aaaatcattg atctcggttt
     3181 gaactatatc tactttgcaa aaaagcatca tagtctttac atggcattgt acattgatga
     3241 acatggggac ggaaagaaaa tgcatgattt ttcatacaat tatttccgtg aaatagttaa
     3301 agaagatgaa agatacaatc aattatcgga tgaatatgtc gatgctctgc atactggtac
     3361 atgggtagca gtgactggct tagcgtcatt gatgtcatca aacatcatgc atccaacaga
     3421 agaacaaatc attgcactga tcaaacggac aattgactcg attttagcat tagataatcc
     3481 tgaaataatc agatagtgtt ctatcagtat gaatttgatc ggtaagtccg ttggctgcaa
     3541 actgtcaatg gacttttttg ctaaaaaaag cgatttctgt tatttatcta ttagaaatag
     3601 gcttgacatt tcgtgaaaaa agtttaaaaa tagtacttac ggaaggttgg cagagtggta
     3661 atgcaccgga ctcgaaatcc ggcgaaccgg ctaataccgg cgcgcaggtt caaatcctgt
     3721 accttcctta tatcaacgat tagaggttgt gaagaagctg tcctgcatca agtaataaga
     3781 acggccaaac aaaaatagct tttcctattt tttcgtttga ttgggcttat taccgcagat
     3841 gcaagctttt gaacaccgtt tgttcggaat aaggggctgt gacaagactt ttgtcacagc
     3901 cccttattgt tgtactttta acctacaata ttttttgatt ccccctgagt actatttttt
     3961 ataaatgcta gtaaatcaat cttctttggc ctttttttga taaaaaaaat actagatgta
     4021 agataaaagt gtaaaaacga tttgttttag gacgggaaac gatgtgtacc gcaatcactt
     4081 atgcaacgaa agaccattat tttggaagga attttgatta tgagatgtct tataatgaag
     4141 tggtcactat tactccaaga aattatcgtt tcgattttag aaaggtaaaa aatttagata
     4201 aacattatgc aatgatagga atagcagctg gggtatccaa ctatccactc tattatgagg
     4261 ctacaaatga aaaagggttg agtatggcag gactgaattt tcctggcaat gctgattata
     4321 aagaattaca agaaggaaaa gataacgttg caccatttga gtttattcca tggatactag
     4381 ggcaatgctc aaccatagac gaagctaaag aattattggc tactatcaat ctagtcaata
     4441 tcgattttag tgaaaaacta cccttgtcac ctttacactg gttattagct gataaagaaa
     4501 aatcgatagt aattgaaagt atgaaagatg gacttcattt atatgataat cctgtcggtg
     4561 tgttgacaaa taatccgcca tttgattatc aattatttaa tctgaacaat tatcgctcgc
     4621 tatcaaatgg aacaccagaa aatcattttt caaaccagat cagcttgaat gtttatagtc
     4681 gtgggatggg aggattgggc ttacctggag atctctcttc tgtttctcga tttgtaaaag
     4741 ccacatttac taaaatgaat gctgcatcag gtgattcaga atctgaaagt atcagtcaat
     4801 tttttcatat actaggctca gtcgagcaac aaaaaggagt atgcgatact ggagaaagaa
     4861 aatacgaata tacgatttat tcttcttgct gtaacgttga taaagggatt tattattatc
     4921 gtacgtatga agacagtcaa attactgctg taaatatgaa taaagaagac ttggatcgtc
     4981 atgaattggt cagttatcca gtcataaaag agcaacagat aaactatgtc aattaattaa
     5041 tgaatagatc cgtttggtat catattttct gatacagtca taattagaca cgatctttag
     5101 gtcgtgtctt tttttgctgg tttttttgga aatagcatcc actaatattt ttttaacacg
     5161 aagagaataa ggtagcctca aaaaagaaaa catggtaaat tagatattgg gactttattt
     5221 tctgtaaaaa cagaaacttt catcttttgt tttcacgtga cgaaattgta agagagaaaa
     5281 tattcgtccg ctatttattc tatgataaga tgcatatgaa tgaaataaac agtaatttta
     5341 tattaggatg tgaaagttaa atgtgcggta ttgtaggttt tgttaacgac aaggacaaca
     5401 aaaaaacaat tattaataca atgatggatc gaatcgttca ccgtggacct aatagttcag
     5461 gagaatatat cgataaacat gttgctttag gattcagaag attaagtatt attgacttag
     5521 agggcggtac tcagcctatt tacaatgaag atcggacaaa gatcattatt tttaatggcg
     5581 aaatctataa ttaccagcct ttgagagaag aattgattgc agctggccac gtttttcaaa
     5641 cacatgcaga tacagaagtc cttttgcatg gttacgaaga atggggaaca gaattattgc
     5701 aaaagatccg cggaatgttt gcttttgcta tttgggataa tgaaaagaat gagttattcg
     5761 gtgcaagaga ccactttggg atcaaaccat attattatgc agaaatgaac ggaacattta
     5821 tgtttggttc agaaatcaaa agttttctgc ctcatcctga ttttaacaaa gaattgaaca
     5881 aagaagcgtt gaaaccttat atgactttcc aatattctcc attaaacggc gaaacattct
     5941 tcaaagatgt ctatcgtttg cctgaaggcc attattatac gtataaagat ggaaaaatag
     6001 acattcagca atattgggat gcagattttg aaacaaaaga aacgcattcc cgacaagaat
     6061 ggatcgaaaa aatcgacgaa accgttcaag cttctattga agcacatacg gtcagtgatg
     6121 tagaagttgg ttctttctta tccagcgggg ttgattccag ttatgtaacc tctgtattga
     6181 aaccagatca ttctttctca atcgggtttg atgataaaac ctataatgaa gcgattgaag
     6241 ccagaaaact aaccgaacta ttggacttag ataatacagc agcagtgatc gacggagaca
     6301 tgtcgttcaa agcattccct ttgatccagt atcacttaga tgaacctgat tcaaatcctt
     6361 cttgcgtacc tttgtacttt ttagcaaatc ttgcttcaca aagtgtgcgg gtagtccaat
     6421 ctggagaagg ggcggatgaa ctgtttgcgg gataccaaac gtatggattc catacgaatt
     6481 caaaattcat ccgtgtgatt gctcaaggat tgaaaaaatt accaaaagga acacgctata
     6541 atttaggacg taaaatcgga aaaatgaaaa atttccatgg acggatccat ttatatgaat
     6601 cacttgctcc tgcaaaagaa tattttatcg gtcatgctcg agtgttcgaa gaatcagaag
     6661 catcagaagt gttaacacca aaatatcaaa cagcaccatc tgtggatgaa atcatgactg
     6721 ttcattacga gaaaacagaa ggaatcaaag atgaagtcaa taagatgcaa tatgtcgatc
     6781 ttcatcaatg gatgccaaaa gatatcttgt taaaggcaga taaactttct atggcaagtt
     6841 ctttggaagt ccgagtacct ttattggata tcgaagtcat gaaattggct caacaaatac
     6901 caagtaagtt tttattgaat caaaacaaca caaaagatat tttcagacaa gctgcgaaca
     6961 aacatcttcc ggaagaatgg tcgaatcgtg tgaaattagg attccctgtg ccaatcaaag
     7021 cttggttgaa agaagaacac ggctacgaac aagtcaaagc attattcgaa gcagattttg
     7081 caaaagaatt ttttgatcaa gaaaaaatca tgaagttgct agatgaccat cacgaaggac
     7141 gtaaagaaga gcaaagaaaa atctggacaa tcttcagttt cttgacatgg tataaagtct
     7201 actttgtaga tgaaacgatt ccgcaagcag aagcaatcga ctatgtgact gtttgatgaa
     7261 aatcaaatat caataaaaaa gaggttttct cctaccgttt aacactgagt gataagaaac
     7321 aatttcaaaa aacagctctt cccgttttat agatattatg acttatggtt cggagttggc
     7381 agaggaacac cattgtttcg aacataaggg ggttgtgaca cgattttcgt cacaacccct
     7441 ttacatatgt tcaataatcg atttcttttt cttttgtccc atctttttta taaatgaaca
     7501 aagttgattc tttgttttgt gctacttgct ttcctttttc gattgcttct ttttttgtat
     7561 caaaatggtt gcttgccttt tttgcccctt tagatttcac gatccattgt tcttcctcat
     7621 attctacgat cacatctgaa tcaagcagtt ttccagcacg aggattcgta tcgtgttcat
     7681 ccgtcttgct tggatttttt tctttttcga aagttcgttt ttctgattca ctagcatctt
     7741 cataccattt ttttgcttga tcaatggcaa ttgggatcgc acggttatca gggtaccctt
     7801 catctaataa tgcatttgcg atgtcgattg ctttttttct tgtcaattta tctaagtttt
     7861 tcattgatgc aggaaagtcc ttcatgttcc atggcatatt agtatccccc tttattaaaa
     7921 aacataatct atataaacaa gtgtaagggt atttatacaa aaagaaaaag attttgtttg
     7981 agaagtaaaa ccgttgtatt tatcgtcaag tagttttact taacaaaatc gtgaaaaaca
     8041 ctagcaatct agaaaaaatc atggtaagat aatcaagtct gagagtcatc ttagaggatt
     8101 cttgtaaaaa tgggagggaa aaacatgcgc gtaaacatca ctttagagtg tacttcttgt
     8161 aaagaacgta actatctaac aagcaaaaac aaacgtaaca atcctgatcg tttggaaaaa
     8221 caaaaatatt gcccacgcga acgcaaagtt actttgcacc gtgaaacaaa ataatgtttt
     8281 taaagttctc aattccttgc tggtcaaaga attgagactt tttttatctc aaaacatcta
     8341 aaatcataag gagcggataa tatgtttatt tccatgtggg cacaagataa aaatggtctg
     8401 atcggcaaaa atggtttgtt accatggcga ttgccaaatg atatgcgttt ctttcgcgaa
     8461 cacacaatgg atcgaatttt agtgatggga agaaagacct atgaaggaat gggggatctt
     8521 tcacttcctt atcgccatat catcgtgttg acaacgcaac caaattttcg cacgaaagaa
     8581 aatgcggaag taatgcattc gatcgatgaa cttttgtcgt atagtcaaac gatctcagaa
     8641 gatatctatg tttcgggtgg tagtcggatt tttcaagaac ttcttcctta cactggtcgg
     8701 atctggcgaa cattgatcga tagcacgttt gaaggcgata cgtatattgg gaatattgat
     8761 ttttcagact tcgcccttat agaggagtat gaggggataa cggatcaaga aaatctatat
     8821 gcccatcgtt ttcaaaaatg ggaaagagta aaaaataggg ttgcttcaaa agaagaataa
     8881 aatctatgcg tctagtttgt taataagaaa aaaagcatct cacgactaat cgtgaagatg
     8941 ctttttttag ttaaaatcaa tttagttttt ctttgatatc attgaccatt tcctgactgg
     9001 caataggtcc gttatatagc caactagatt caggtccgaa ttgcagtaca tgattgtctg
     9061 tcactgcttt tagattcttc cagcttggtt cattgaacat ggcagcatct ttgtcactgt
     9121 tcacaaggat gatgtagtca gcatctaatt cagctagctt ttcagaggaa acagcagacc
     9181 agtcagaagt ggcttttttg gagacctctt ccactagatc aggaacacca aattccagat
     9241 cgtcgtatac gattcttcca cttgaccgat ttttgctgac cataaaggct gagttgttgg
     9301 tcacccacaa cactgcagct gtcttgttct cgattttatc tgctagttct tctcgagttt
     9361 tcttgatgga tttttgataa tcggaaatga ttttttctgc ctctttttct tttgccaaag
     9421 ctttgccgac atcttttagt tgttcttccc atgtgacatc tgttccgttt tttacaacat
     9481 aagttggcgc gattttgcta tattgttcgt atttcccgcc ttcgacagta gcggaagaag
     9541 aaatcaacag caaatcaggc tcaaagctta acactttctc ataaggaagg tcataagaaa
     9601 tcgttgggac atcctttaaa tcatcttgca gataatcttg aatggaacca ctgccaacag
     9661 tccactgggc gataggtttt tcccctagtg caatcagata gtcttctaag taactgccga
     9721 tgatccgttt gggcgcgttt ggaatctcta ctttatgtcc aagtgtatct gtgactgtat
     9781 gagtactatt ggatgaagtc gtcgaattgg cagttgtagc tgtttcttta tctgaagagg
     9841 gggagcaagc gctcagtact ccagccagca gcaatatgct agataatgtg aatacttttg
     9901 tttttaattt catttctttc ctcctaacat aattgagaat ctttctcatt ggttattatt
     9961 cctgatattt cgaacgctgt caaccgcttc atttggctat agatatgaaa gtagcgcttg
    10021 acgaaatgtt gaaacagcgt cataattgag aatgattttc agcgaaagga tgggtgaaat
    10081 gacgagatta agagcagctg acattgcagc cggctacgaa aacaaaaaaa tcattgaaaa
    10141 catgtctgtt acgattcctg aagggaaaat cactagtttg attgggccaa atggaagtgg
    10201 aaaatctact ttattgaaaa gttttacccg tatactacct gtgaaatcag gaaagatcat
    10261 tgtcaataac tctgatatct ctacatacag ttcaaaagaa cttgcaaaaa aaattgcatt
    10321 attagcacaa actagtgaac accctacagg tatgacagta gaagaagtag tatcttacgg
    10381 acgataccct tatcaaaaac tgttcagtgg catggacacg caagatcatg aagcaattga
    10441 gtgggcgttg gatgctacag gtctagctgg atttcgtatg cgggatatcg caacgctgtc
    10501 tggtggacaa acccagcgtg tctggatcgc tatggcgctt gctcaggaaa cagatatctt
    10561 gatcttggat gaaccgacaa catttttaga tccggcacac caattagaga tcctacagtt
    10621 attgactgaa atcaatcaaa tgaaacaaac aacgatcttg atgtcgattc atgacattaa
    10681 tcatgcttca cgtttttcag attatttatt agcaatgaaa aacggtaagt tgatcacaga
    10741 aggtacacct gaagaagtga tcacaaaaga gtgtatgaaa aaaatatttg agattgatgc
    10801 aacgattgtc acgcttccaa gaacaaataa accagttttt ctaacctatg atttgttgcc
    10861 aaaagagcag gaggatcaat tatgaggact atgaagaaaa atagactcct gcttttggtc
    10921 tgttttgttc ttttgattat tttttttgtt gctctttcaa atggcgcagc gactgtggga
    10981 aaagcagatt tttggcaggc gttgtttgat tttgatagat caaaacaaag tcaccaaatc
    11041 atacgaaata ttcgtttgcc tcgtgtggcg ggagcatttc ttgtagggag ttgttttgca
    11101 ttgagcggtg cattgatgca aggagtgaca agaaatcctt tagcagattc aggcttacta
    11161 ggaatcaacg cgggtgcttc gttggcaatg gcccttagtt ttgctttttg gcctaaagct
    11221 tctgattttt ctgtgtttct aatgtcattg gcaggttctt ttgctgtaac tttgctcgta
    11281 tttggttttt tgcagttttc gaaaatgggc atgaatcctt tacagctgat tttagctggc
    11341 gtagctatta gttctttttt tactgctatc agccaagcac ttacccaatt ctttaatctg
    11401 cagcaggatc ttgctttttg gtttgtaggc ggagctgcca atatcacatg gaaacaatta
    11461 gcagtattgt ttcctttata tatacttgct gttttggttt ctttttcatt aggaaaagca
    11521 atatctttga ttcatttgag tgaaacacat gcattagctc ttggaagtca tcctaaacga
    11581 acgcggttga ttgttttctg tctagttgca gtgttagcag ccattgccgt ttcgttagtc
    11641 ggacctgttt catttattgg gctgttggtt ccgccagttg ttcgcggaat ggttggaacg
    11701 gactatcgtt atgttctacc tgtctctttt ttagttggcg gtggattggt tatgttggcg
    11761 gattttacag cgaggatgat caatccccct tttgaaacac cttttggatt agtcattgca
    11821 ttgattggcg ttccgttttt actgatacaa gtaaggaggg aacaacggtg aaaaaaatat
    11881 attggacgtt ctatttcttg ctttttgtac ttgtcatcct gtctataggt agtttaatga
    11941 ttggtacgcc ttttatttac ctggatcagc tttttgccac gttcataggg aaagaatcat
    12001 cttctttggc gctgattgtt agagaatttc gagttcctcg attactgatc agtttattag
    12061 caggagcttg tcttggcgtt tctggttata tcttgcaagg agtaacaaga aatgaattgg
    12121 cagattctag tatattgggc attaatgccg gagcaggatt tttagtgatg ctttatttag
    12181 gtttcttttc gcactattcg ctgccgtttc tattgctagt tgtagccttt ctaggtggtg
    12241 ttttagctgc ttgctgtgtc tatcttgcgg cctatgatcg aaatggcttt ttgggtatga
    12301 atcgaatctt attgtccggt attgccgtta atgccggcct ttctgcatta acattgctat
    12361 gcacgatcca attatctaag gaaaattatg gatttgtgaa tgcatggtta gcgggatcaa
    12421 tctggggtgc gagctggcct tatgtttggg cattgcttcc atgggcggtt atcttaattc
    12481 cgctaggagg actttatgca cagaggattg tattgctatc atttggtcaa gaacgcgcgc
    12541 aaggattagg ccttaacgtg gctaaaagtc aaaaaatcct tttgttgtta gctgttgctt
    12601 tggcaagcgg tagtgtagcg gtgacaggta gtttatcttt tgtaggacta ctagctcctc
    12661 atgctgcgaa gctagtcgta cgtaaagaaa ataactggac ctttgttctg agcggtttgt
    12721 tcggttccat ttttctgctt agtgcagata tggttgctag agtgattcta ctaagtgggg
    12781 aagtacctac aggaattttg attgcccttt ttggtgcgcc atattttctg tatgcacttt
    12841 ttatcagaca aaaacacgcg ctgaacagtt gaaattcatt aaaaaaggaa ggccaatcat
    12901 ttctaggtct tctttttatt ctatttatgc atttatttga aagttcgatt gctatttttc
    12961 tttttttcct ataccatttt atgagagaag gaagggtgag tttttggacc aattgaaagt
    13021 aagattacgc cggaaaggaa tcgaaaagct gaaaaatctg gccaaaagtg gcaagaaaaa
    13081 ggaaaaagaa cagcgaatct ataaacagct attcgccagc gagtactgga agtcagcaaa
    13141 agtgataggg acgactttat cgaatgaatt tgaactggat acacagccgg ttatccaacg
    13201 agcagaaaaa gaacaaaaga aaatagttgt acctaaaact ttgccgaaac gtcagatggc
    13261 gttttatgaa atggatgaac agtccgttct tgtacgttcg agttttggtg ttcttgagcc
    13321 acaatcagag aagctctatt tgccggagat gatcgatctc ctcattgtac caggtattat
    13381 ctacaataga gccggttatc gaatcggttt tggcggtggt tattacgacc ggtatttaaa
    13441 taattttcat aaaaaaacat gtagccttgt ttttcgtgaa caattgaatg aacaatggca
    13501 accggagagc tttgatcaga agatccaaca attgtttatt gactaaaatg aaatgggagg
    13561 ttttcatgaa ttatcaacag caaataaaat tgcgtgcatg gttaagacga ccgttatgga
    13621 cctatgcttt tttagggata cagacagcgg tcttcctcat gatggaatta tttccgcgac
    13681 ttgagatccc ttattatgcc ggaatgtatg gtccttatct tgttcacttc aatgaatggt
    13741 ggcgtttagt gacaccaatc ttcatccatt ttggactgat gcatttcgta atgaactcac
    13801 tgatactgta ttttatgggg cagcaaatcg aggcaatcta tggccattgg cgttttttct
    13861 tgatttatat gttcagcggg ataatgggaa atacagcaag ctttgctttt aacgaagcga
    13921 atgttctgtc tggtggtgca agtacttcga tattcggttt gttcggtgcg ttgtttatct
    13981 tagggtttca ttttaaacat aacccagcga tccagcagct agtcagacat tttcttttat
    14041 ttattgtgtt gacatttgtt tttggtcttc tggatacctc tgtagatgtt tggggacatg
    14101 tcggtgggct aattggcgga ttagtgttag ggaatgtttt gggtctaccg aaacaacaga
    14161 caagctattc gatacaccaa aggattcttt ctacactagt ttttgtattt ctttttgtaa
    14221 tatgtatttt actgggttta aaaaaatatg gtttacttgt ataatatgaa ggaagtgctt
    14281 aaagatggag agcttatatg atgtacagca gctgttcaaa aaatttggca tctttatcta
    14341 tgtgggtgca cgcatctatg acattgaatt aatgactatc gaattgcgta atctgtatga
    14401 tagccgtttg attgatcatg atacatacat gacagcgtgg cgcatattaa aaagagagca
    14461 ccgaattgaa gaaagccgaa agaaaggaaa tttgtaatgg acaagaagat tattgggatt
    14521 gatttaggtg gaacaacagc aaaatttgca attttaacac cagaaggaga gattcagcaa
    14581 aaatggagca tcgataccaa tattttggat gatggtaagc atatcgttcc tgaaatcatt
    14641 gaatcaatca atcatcgttt gaatttatat aatatgaaag ccgaagactt tatcggaatc
    14701 ggcatgggta ccccaggaag cgtggacagc gaagctggaa cggttattgg tgcgtataac
    14761 ttaaactgga cagaagttca atttgtgaaa aaactgatcg aggcaggaac aggaatcaaa
    14821 ttcgctatcg ataatgacgc gaatgttgct gctttaggtg aacgatggaa aggtgctgga
    14881 gaaaacgatc cagacgttgt attcgttact ctaggaacag gtgttggcgg aggtatcgtt
    14941 gctggcggaa atttgattca cggcgtgagc ggagctggag gagaaatcgg acatatcact
    15001 gtggatcctg aagggttcga atgtacttgc ggtaaacgcg gatgtttaga aactgtatct
    15061 agcgctacag gtgtggtacg cctaggtcgt ttcttggcag aagaatacgc aggggattca
    15121 aaactgaaag caatgctaga caacggagaa gaagtaacaa gtaaagatat ctttgaaatg
    15181 gctcaagaag acgatccgtt tgcattgatg gttgttgatc gcgtatgctt ctacttaggt
    15241 ttagcttgcg gaaacttggg gaatacatta aatccgtcaa gtatcgtgtt aggtggagga
    15301 gtatcggcag caggagaatt tttacgtagc cgtgtagaga aatattttaa agaatttact
    15361 ttcccgcaag taagagaatc aacaaaaatc aaattagcag agcttggtaa tgaagctggt
    15421 gtcattggtg ctgcctcatt agccttgaaa tttgcttaaa aaaacggagg cattacatgg
    15481 ttttatgggt aattaatatc attttgctat tgattgtgct tgcaatcatc ctttgggaag
    15541 tctatctacg agtaatggcc aaacgttcag caacgacttt gacagaagaa gaatttcgag
    15601 aaggaatgag aaaagcacaa gtaatcgatg ttcgcgaaaa agatgtgttt gatgctgggc
    15661 atattttagg tgcaagaaac atcccttaca ctgttttgaa agattcatta ggatctattc
    15721 gaaaagacca gcctgtctat atttacgatc aaaagaaaag tttgagcatc cgtacagcaa
    15781 ataaattacg taaagcaggt tatcaagaga tttatttact taagggtgga tatgacggat
    15841 ggtctggaaa aatcaaaagt aaaaaagtat aaaacagatg attgtatgac agctttaaaa
    15901 accgaatcaa tcattagatt ttgaccaaga tcacatgaac ggcgcaatgg atagttgtgc
    15961 cgttcatttt ttttattttg tgctattatc tacatatata agctaagcga aaaggtagaa
    16021 agaaggaaac aactatgatc aaattaaaac gtgtttacct tgatgctgaa aaagaggatg
    16081 gctatagaat actcgtggat agattctggc cacgaggaat ttcaaaagaa aaagcgcagt
    16141 tagatctgtg gttgaaagaa attgcaccta gtaatgaagc acgcaaaact tttgaccatg
    16201 taccagaacg atttgcttca tttgcaaaga aataccagca agaattgatt ttcgaagaag
    16261 aaaagaagca agctattttt aaactcagtc aacttatgaa agaacatcca gtcattactt
    16321 ttgtttatgg tgcaaaaaat gaacatcaga accatgctgt tttattgaaa aaatttatcg
    16381 aaatggaggc atgcaaatga aaatagggat cattggtgcc ggacctagag gattaagtgt
    16441 agcagaaagg attctccata ataatcagga cagacattct cttgaacttt ttcttttcga
    16501 tcccaatggt cctggaggtc gagtttggcg gttgaatcag ccaccggagt tattaatgaa
    16561 ttctgtttct caacaagtga cgctttttac agatgaaaca ttgtccagtg gaggattggt
    16621 aacacaggga cccaacttgt ttcaatggag taaaggacct gctacagatt atatcgaaca
    16681 gacggtaacc aaaaataagc aactaattat agaagaaatc aatcgtctgg aaaaagatca
    16741 gcaatctact cgatgtttat acggactcta tcaacgctgg tttttctcaa agttgcaaga
    16801 agaatttcca gcttctattc agtttgttta ttctttagta aaaaaagtaa gcaaaagaaa
    16861 agatggttat cttttacaga cagatcagca aagctattct atcgatcagc ttgttttagc
    16921 aagcggtcac tgggaaaatg atgagacaga agaagaaaga gtgtgggtag actacgcaaa
    16981 gaaaaatgtt ttactgtatc agcttccttc taatcctgca gatgtagatc tttcgaagat
    17041 accgcctaaa gaaaatgtct ttttaagagg tctaggctta agtttttttg attatgtcgg
    17101 attgtttaca gtatcaagag gagggcgctt cgagaacgaa aagggaaaac tggtctatca
    17161 tccatcaggc aaggaaccga ttgtatacag tggatcaaga agggggctcc cttattaccc
    17221 tcgaggaaga aatcaaaagc aaggcggagc aatggcatgg ccacggttac taacaaaaga
    17281 aaacctggag caatggcatc agtcgggatt tttgactggc gaaacatttt ttgactatct
    17341 gaaaaaagac gttgagttgt tttattacaa gaaaatcatc gaagaatatc acttgccgat
    17401 tccaaagaaa aattttgagc atgattttct ctccctctct tcagaggagt gtctcggaaa
    17461 gcatccagca ctaatccctt atagatggga ttggtcgtat ttagaaactc ctcttgccta
    17521 tcagaatgag cccttttcag aagctagccg aatatttatt gaagggcaga taaaagaagc
    17581 ggaaaaaggc aattgcacag gagcatttgc ttcaacattt gatgcattaa aagactggcg
    17641 tgatccaatc cggcaggcta ttgaatggca cgttttttct gctaaggaat acaaagaaat
    17701 actatgggga tggtttactc atctaaatag ctttttaacg atcggtccgc caatcattcg
    17761 gacgaaagaa ttagcagcac tgatagatgc gggtattttt catttgatcg aaccgccatt
    17821 tgatattcag caaaagcaca accgctatgt tatttctcta caagataaag aaatcagcag
    17881 tcgctatttg attgaagctc gattgccaca aacaagcttt tctcgttcaa gtaatcctgt
    17941 cataaaaaat ttgaaggaaa acaaacttgt tcgccctttt tcattttcag ataatacagg
    18001 aagttatcat tcagaagcta tagatgttga gcgaggtacg aatcgcgtga ttgatgcttc
    18061 aggggaaatc cagtctgact tttattgcta tggaatacct gttgaaggtg tggattggct
    18121 aactgcagca gtagctagac cttatacaga tccatggaat ctgagacagg cagataaaat
    18181 agcacaactt atattgactg acaaataaaa atacgcatga tgacttatct gttttttaga
    18241 aagtcttcat gcgtattttt aaataattat taacgagaaa cgcgtttacg cattgctttt
    18301 ttacggtttt cttcgatcat tgcttctttt tcgtcaattg gatcgatcac tgtttttttc
    18361 acagaagcaa ccaaaccagc aacggcagcg gctgttgcca gacttcctac taatattcca
    18421 gatacaaatt ttttcattga atccacctca tttattattt gttatatcta ttatggaaca
    18481 aaagagaaaa aaaggctagt tttacgtttg tttgtaaacc tttgcaaaaa atccttgaaa
    18541 atgtttacta aatgttttat tataaattta ccatatgaat ttacgaaatt ttactttgtt
    18601 tgttttcacc ataaaagtaa acttcgtttt ttaaattata tagaatcaac aaacacaatt
    18661 ttactaatca aaggagtgtt ttttatgaca attttagtac taggcggagc cggttatatc
    18721 ggttcgcacg ctgtagacca gctagttcaa aaggggtatc aagtagctgt ggtagataat
    18781 ttgctcacag gtcacaaaca agcagtacat ccagatgctc atttttatga aggagacatc
    18841 cgggataaag aatttctacg ttccgtattt gaaaaggaac cgattgaagg agtaatccat
    18901 tttgctgcaa gctctttagt aggagaatct gtcgaaaaac cattgatgta tttcaacaac
    18961 aacgtatacg gtatgcaaat cttacttgaa gtcatgcacg agttcaacgt gaataaaatc
    19021 gtattctctt ctactgcagc aacttatgga gagccgaaag agtctccaat cacagaagat
    19081 acaccagcta atcctaaaaa tccgtatggt gaaagtaaat tgatgatgga aaaaatgatg
    19141 aagtggtgtg accaagcata tggcatgcgc tatgtcgctt tgcgctactt caatgtagcc
    19201 ggagcaaaag cagatgcatc tatcggagaa gatcatacac cagaaacgca tcttgtccca
    19261 atcattttac aagtagctct tggtcaaaga aaagccttgg ctgtttatgg agacgattat
    19321 gatacaccag atggaacatg tatcagagat tatgttcaag tagaagattt gattgctgca
    19381 catattttag ctttggaata cttgaaagaa ggaaatgaaa gcaacttctt caatttaggc
    19441 agcagcaaag gatactcagt caaggaaatg ctagaagcag ctagagaagt gacaggaaaa
    19501 gaaattccag cagaaatagc accaagaaga gctggagatc ctagccgatt agtcgcttca
    19561 agtgaaaaag cgagagagat tctgggctgg aaacctgaat acacagatat caaagcgatt
    19621 atcaaaacag catgggattg gcatgtcagc catccaaatg gttatgaaga ataggggaat
    19681 gagatatgtc aatcagtcaa acgatttgtg attttgccac acttgcgatc gaagctggcg
    19741 gatggatgga aatggaccgc ttatatttac agaatcgtct tttagggatg gtaggagaag
    19801 atgctctaga aagcattgaa gtaagaccag ttaaggaaga atctgtagac ttattggatc
    19861 agcttgtcgc taaggcaaga gaaaatggtg ttctttcaga attgccagca gaacaagaga
    19921 tttttgaagc gcaattaatg gattttctca caccgccacc ttcagtcgtc aatgcattct
    19981 ttgcacagca ctattcgaaa agcccagagg aagcaactga gtatttcttt aatttaagta
    20041 agaaaaatga ctacatcaaa acaagagcca tcgcaaaaaa catcaaattt ccggcagagt
    20101 cggagtatgg tgaactggaa atcacaatca atttatctaa gccagaaaaa gatccaaaac
    20161 aaatcgcagc agaacgcgag gcggtaaaag tagactatcc tacctgcatg ctttgcatgg
    20221 aaaacgaagg atatagaggt cgcttgaact atccagcccg caccaatcac cggattattc
    20281 gaatgaatct agacggtgaa agctggggat tccaatattc cccttatgct tattacaatg
    20341 aacattcgat cattttatca gaacaacacc gcccgatgaa aatcacaaag gaaacatttg
    20401 ctagactgct tcggattact gaagtcttgc ctcattactt tgttggttcg aatgctgact
    20461 taccaattgt tggaggatcg attctttctc atgatcatta tcaggcagga cgtcatgaat
    20521 ttcctatggc aaaagcaccg atagaaaaac ttatcaaact agaagattat ccgcaagtca
    20581 tcgcaggagt cgtgaaatgg ccgatgtctg tgatccgttt gcaatcaaca gagaaaaact
    20641 tgcttattga agcagctaac tcagtattgg aaaaatggaa agcatattcg gatgaaacag
    20701 tatctatcag agctttttca gcagatggta caccacacca tacgattacg ccaattgcac
    20761 gaagaaggga caattacttt gagttggacc ttgtccttcg agacaacaat gtttcagaag
    20821 aacatcctga tgggattttt catccacata aagatgtcca acatatcaaa aaggagaaca
    20881 ttgggttgat cgaagtaatg gggcttgctg ttttaccacc tcgtttgaaa ccagaactag
    20941 tagaagtaga gcatttctta ttaggagaag aaaaccagat ggctgaatat catcgtccgt
    21001 gggcagaaca attaaaaaaa gaacatcctg atttgacgaa agatacggtc accgatgtcg
    21061 tacaaaaatc tgtaggtcag gtttttgcac gtgtactgga agacgctgga gtattcaagc
    21121 gagatgaaaa aggacaggca gcgtttgaac gatttactgc gattttataa acgtaaaaaa
    21181 ggaggagaga caaatggcaa caattaaaga cattgcgcag ttggcaggag tttctcctgc
    21241 tactgtttca agagttctca actatgatcc tgatctttcc gttggcttag ccacaaagca
    21301 aaaaattttt gaagcagcag aacgcttaaa ttatacgaaa catcgtaaaa atgcagcaaa
    21361 tactaagcaa acgcttcgcc tgatccagtg gtacgacgat acggaagagc ttgaagatct
    21421 ttattattta tctattcgcc taggaatcga aaaaaaggca gaagaagcaa acgttacttt
    21481 attgaaagaa acgtgggatt ccattagtga tcttccggca gatggaacga tcgcactagg
    21541 aaaattcgac gaagctcagt tattagaaat gaaaggatcc caaaatgcct tgcttttggt
    21601 agattctgac ggcacttctc aaggaatcga ctctctagtt gtcgacttta agtcaagtgt
    21661 tcaaaaagtc attgaacact ttgtctccca taaagcgcaa catatcgcta tgcttgctgg
    21721 aacagaatat acgaagaaaa accatcagcg gctaaaagat cccagaatta tggcatttca
    21781 acaagttttg gcagaaaaag gctacgacga aggtccaatt atcgaagcag atttttctgt
    21841 cgagtcagga tatcaagcag tgaagcagta tttaaaaacg aatccgcatg ttcctgatgc
    21901 gctttttgca gctaatgatg cattagcgat tggcgcactg aaagcgattc aagaaaaagg
    21961 tttagtggta ccagaagata tttctgtgat gggcttcaat gatgtcagtg tagcgaaata
    22021 tgtaacgccc tcgctttcta ctgtaaaagt ttataccgaa tggatgggag agctagcagt
    22081 ggagacgatt cttcaattaa gtaattccaa agcaccggtt tcaagaaaga ttattgtgga
    22141 aacagagttg attattcgag aatcaacgaa ataaaagatg gaaaagctgt gggaaaatgg
    22201 agtgataccg cagctttttt gtgtttcagg aaggggcaaa atttgcccgc cagcagataa
    22261 tcatgtatta ttgtactagt tttgcctttg gtattttcat cctcagaata aaaaagtatg
    22321 ctacaatgga aggcgagaat gtcatagcag aaaggatcaa atatatgatt acattaggaa
    22381 tagatactgc aaaccaaact ttggcgatcg gtgtggtaga agacgaacaa attcttggac
    22441 aaatacaaac aaatatcaaa cgcaaccaca gtgtaacact gatgcctgca atcgaccaat
    22501 tgtttgcaga tcttcaaata agtccaaaag acattgaccg aatcgcggtt tctgatggtc
    22561 ccggttctta tacagggctt cgtataggtg taacgacagc aaaaacgatt gcttatacat
    22621 tagataaaga gctagtagga gtatctagtc tgaaaaccat cgcggctaat tgcatcggtg
    22681 tggataatat tattgtgccc atgtttgatg ctcgaagaaa aaatgtatac gctggggcat
    22741 accgctggaa agaaaataga ttagaaactg tacttgaaga tgtacatatt tctgcttctg
    22801 atttgttcac acaactacaa aagctgaatg aagctattta tttcgtcggg agtgattgtc
    22861 acaaattctt ctctgaaata aaggaaatat tgcctagtgc gatgattaat acagttccag
    22921 catgggatat tccaagtggt gtaactgtgg ctcaattagg tgctttagtt gaaccagttg
    22981 cagagaaaca agcttttctt cctcgctatt taaaacgagt agaagcagaa gaaaaatggt
    23041 tggagacgca cacacctgga gacgaaagtt atgttgaaaa aatttagagc gtatgccaga
    23101 agcttttttc aagataaaat gatttataca gaaaaaacag tcgaacaaat cgatcgtcgt
    23161 tacgtactaa gagaattgac tacagaagat atcaaagagc tgctggatgt cgaacgagaa
    23221 gtctataatg gcgaattgcc atggacaaaa agtgccttta tatcagaatt gaagtcaccg
    23281 cttcctcatc tatatctagg tatctttgat aatggaagaa tgattggttt tatcggttca
    23341 agagcgttcg gtttagattg ccatatcacg aatattgccg ttcgaccggc atatcagcgt
    23401 aaacgaatcg gttcgctgct tattgacgaa atcgagaact ttgctataat gaatcgctgt
    23461 gagacgatgt cattagaagt gcgtatgagt aatcaagatg cacaaagact ttatcggaaa
    23521 ttggggtttc aagcaacaac gatcaaaaaa agatattaca ctgaaacaaa agaagatgcg
    23581 ttggatatga taaaactttt agaaaatgag tgagattagt tgatttgcag aaaaaaagat
    23641 tttgatgaag cgtacttggc agaatgcgta tggatgatta gcgaaaatgc ttatccacat
    23701 ggttcacctt ggacgaaaat gcagtttttg gcagacatcc aacagcctca ctctgattat
    23761 ctgcttttag ctgaagataa ccagatctgt ggctttattg gatactcgaa agtaatagat
    23821 gaagtcgaga tcacaaatat tgctgttgca gtttcagaac aaggaaaagg gcatgcacgg
    23881 catttgttac aattactgat tacggaacaa ataaaagatt cgctgcatgt ttttttagaa
    23941 gtacgtctat ctaatgaagc agcaagaaaa ctttatgaat cagaaaagtt tcgtatatta
    24001 ggtaaacgga aatcatatta tcgcaatccg acggaagatg cgatcattat gagcagaaag
    24061 ttaaaaacgg agacgatgaa atgaatgaag aattgattct ggcgattgaa tcaagttgtg
    24121 atgaaacgag tgtagcggtc gttagaaatg gaacagagat cttatctaac atcgttgctt
    24181 ctcaaataaa cagtcataaa cgttttggtg gcgtagtacc tgaagtagct agccgccatc
    24241 acgtagaaca aatcacctta tgcttggaag atgcactggt agaagctggt gtgtcggcag
    24301 aagatttatc ggcagtcgct gtcacttatg gaccaggact tgtcggctcg ttgctgattg
    24361 gtatttctgc agctaaggct tttgcttggg cacaccaatt gccgcttatt cctgtaaacc
    24421 atatggcagg gcatatctat gcagcccgtc tggttaaacc attccagttt ccattgatgg
    24481 cacttcttgt tagcggagga catacagaat tagtctatat gcaagaagat ggatcatatg
    24541 aaatcattgg ggagacaaga gatgacgcag caggagaagc ttatgacaaa gttggaagag
    24601 tattaggtct ttcttaccca agcggaaaag aaatcgatca gctagctcat cagggaaaag
    24661 ataattacca ttttccgcga gcgatgatcc atgaagataa ttatgatttc agttttagcg
    24721 gattaaaaag tgcatttatc aatttggtgc ataatgcaca acagcgtgga gaagatctag
    24781 acaaaaatga tttagcagct agctttcaag caagtgtgat cgatgtattg attactaaaa
    24841 cacttcgagc ttgtcaaaat tatccagtca agcaactgat tatagctgga ggtgtagcgg
    24901 ccaatcaagg gttgcgtgaa ggattgcagg atgctttggc aactaaactc cctgaggtag
    24961 aactagtgat tccccctctt cgactttgtg gagataatgc tgcaatgatt ggagcagcag
    25021 cacatgtgga aatgcaaaaa agaaattttg ctagctatca gttgaatgct gatccaagct
    25081 tggttttatc tgaataaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
    25141 cccaggaaaa acggcttctc atgtttttga cctggctgtt cttattaccg cgtatgcaag
    25201 cttttgaacg ccgtttaatc aagagttcgt aagcctgtcg tttaactctg attcacaagg
    25261 aacaatttca aaaaacagct cctcatgttt atgaacatta tgacttgtga ccgaagagtt
    25321 gacaggtaca caccgtttaa tcgaaatagg gctgtgacac gtttttgtca aagccctatt
    25381 ttttacatac ttttaatcat tattgttatt ttgctatttt ttacagctta tttatcatta
    25441 tccgttcctc tgattgccct cactgagtaa tcgtttattg tcgaggaata tcgaataatc
    25501 tagatcggat gaggacaaga cgcttcatct agttaatcta acagtatttt ttgcaaacag
    25561 tgaatattac ataactgaaa cacacataga aaatctttga gtcttgtcca gtgatctaaa
    25621 actgccggat ctttcagaag ttttagggag gaaaataagc agtgaccctg at
//
LOCUS       DACDNO010000039        25640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDNO010000039 DACDNO010000000
VERSION     DACDNO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.039"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            127..3654
                     /locus_tag="IWI22_001702"
     CDS             127..3654
                     /locus_tag="IWI22_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9169094.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IWI22_001703"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IWI22_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9169095.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IWI22_001704"
     CDS             7442..7783
                     /locus_tag="IWI22_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9169096.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IWI22_001705"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IWI22_001705"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9169097.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IWI22_001706"
     CDS             9140..11560
                     /locus_tag="IWI22_001706"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9169098.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IWI22_001707"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IWI22_001707"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9169099.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IWI22_001708"
     CDS             12994..13731
                     /locus_tag="IWI22_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9169100.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="IWI22_001709"
     CDS             13745..14560
                     /locus_tag="IWI22_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9169101.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="IWI22_001710"
     CDS             14573..15220
                     /locus_tag="IWI22_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9169102.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="IWI22_001711"
     CDS             15236..15892
                     /locus_tag="IWI22_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9169103.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="IWI22_001712"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="IWI22_001712"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9169104.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="IWI22_001713"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="IWI22_001713"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9169105.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="IWI22_001714"
     CDS             18234..18752
                     /locus_tag="IWI22_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9169106.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="IWI22_001715"
     CDS             18859..19359
                     /locus_tag="IWI22_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9169107.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="IWI22_001716"
     CDS             complement(19639..20559)
                     /locus_tag="IWI22_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9169108.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="IWI22_001717"
     CDS             20724..21737
                     /locus_tag="IWI22_001717"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9169109.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="IWI22_001718"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="IWI22_001718"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9169110.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="IWI22_001719"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="IWI22_001719"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9169111.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="IWI22_001720"
     CDS             complement(23377..24138)
                     /locus_tag="IWI22_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9169112.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="IWI22_001721"
     CDS             24327..25421
                     /locus_tag="IWI22_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9169113.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25507..>25640)
                     /locus_tag="IWI22_001722"
     CDS             complement(25507..>25640)
                     /locus_tag="IWI22_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9169114.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8493 a         4442 c         5407 g         7298 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tataggttat
    25501 gatttgtcaa attaagctag aataaaatgc ttcttgtaca tagctcaaaa atatttcgat
    25561 tggtgttctg taattcaatg attttcttgg aatatgatta cgttgattgc tgacactgga
    25621 aataaatgtc tgattcactt
//
LOCUS       DACDNO010000040        25632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDNO010000040 DACDNO010000000
VERSION     DACDNO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.040"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(74..922)
                     /locus_tag="IWI22_001723"
     CDS             complement(74..922)
                     /locus_tag="IWI22_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9169115.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1146..3086)
                     /gene="thrS"
                     /locus_tag="IWI22_001724"
     CDS             complement(1146..3086)
                     /gene="thrS"
                     /locus_tag="IWI22_001724"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9169116.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3423..3602)
                     /locus_tag="IWI22_001725"
     CDS             complement(3423..3602)
                     /locus_tag="IWI22_001725"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9169117.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3649..5472)
                     /gene="cadA"
                     /locus_tag="IWI22_001726"
     CDS             complement(3649..5472)
                     /gene="cadA"
                     /locus_tag="IWI22_001726"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9169118.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5750..8029
                     /locus_tag="IWI22_001727"
     CDS             5750..8029
                     /locus_tag="IWI22_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9169119.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8122..8769)
                     /locus_tag="IWI22_001728"
     CDS             complement(8122..8769)
                     /locus_tag="IWI22_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169120.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8948..9667)
                     /locus_tag="IWI22_001729"
     CDS             complement(8948..9667)
                     /locus_tag="IWI22_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9169121.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9798..10949)
                     /locus_tag="IWI22_001730"
     CDS             complement(9798..10949)
                     /locus_tag="IWI22_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9169122.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11166..11906)
                     /locus_tag="IWI22_001731"
     CDS             complement(11166..11906)
                     /locus_tag="IWI22_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9169123.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11910..12266)
                     /gene="rsfS"
                     /locus_tag="IWI22_001732"
     CDS             complement(11910..12266)
                     /gene="rsfS"
                     /locus_tag="IWI22_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9169124.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(12290..12883)
                     /locus_tag="IWI22_001733"
     CDS             complement(12290..12883)
                     /locus_tag="IWI22_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9169125.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDVEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12867..13514)
                     /locus_tag="IWI22_001734"
     CDS             complement(12867..13514)
                     /locus_tag="IWI22_001734"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9169126.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13527..13838)
                     /gene="yhbY"
                     /locus_tag="IWI22_001735"
     CDS             complement(13527..13838)
                     /gene="yhbY"
                     /locus_tag="IWI22_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9169127.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13854..14963)
                     /gene="yqeH"
                     /locus_tag="IWI22_001736"
     CDS             complement(13854..14963)
                     /gene="yqeH"
                     /locus_tag="IWI22_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9169128.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14960..15487)
                     /locus_tag="IWI22_001737"
     CDS             complement(14960..15487)
                     /locus_tag="IWI22_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9169129.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15714..16499)
                     /locus_tag="IWI22_001738"
     CDS             complement(15714..16499)
                     /locus_tag="IWI22_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9169130.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(16492..17361)
                     /locus_tag="IWI22_001739"
     CDS             complement(16492..17361)
                     /locus_tag="IWI22_001739"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9169131.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17362..18741)
                     /locus_tag="IWI22_001740"
     CDS             complement(17362..18741)
                     /locus_tag="IWI22_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9169132.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18749..19168)
                     /gene="fabZ"
                     /locus_tag="IWI22_001741"
     CDS             complement(18749..19168)
                     /gene="fabZ"
                     /locus_tag="IWI22_001741"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9169133.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19193..19669)
                     /gene="accB"
                     /locus_tag="IWI22_001742"
     CDS             complement(19193..19669)
                     /gene="accB"
                     /locus_tag="IWI22_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9169134.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19672..20907)
                     /gene="fabF"
                     /locus_tag="IWI22_001743"
     CDS             complement(19672..20907)
                     /gene="fabF"
                     /locus_tag="IWI22_001743"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9169135.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKRDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20926..21663)
                     /gene="fabG"
                     /locus_tag="IWI22_001744"
     CDS             complement(20926..21663)
                     /gene="fabG"
                     /locus_tag="IWI22_001744"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9169136.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21675..22592)
                     /gene="fabD"
                     /locus_tag="IWI22_001745"
     CDS             complement(21675..22592)
                     /gene="fabD"
                     /locus_tag="IWI22_001745"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9169137.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22699..22923)
                     /locus_tag="IWI22_001746"
     CDS             complement(22699..22923)
                     /locus_tag="IWI22_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9169138.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22976..23938)
                     /locus_tag="IWI22_001747"
     CDS             complement(22976..23938)
                     /locus_tag="IWI22_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9169139.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23948..24388)
                     /locus_tag="IWI22_001748"
     CDS             complement(23948..24388)
                     /locus_tag="IWI22_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9169140.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24694..>25632
                     /locus_tag="IWI22_001749"
     CDS             24694..>25632
                     /locus_tag="IWI22_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9169141.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMIASADVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQSEIELLNK"
BASE COUNT         7117 a         5646 c         4439 g         8430 t
ORIGIN      
        1 ttgatggatc atttatttgt ggaaaactac gttttgttat tctaaaaaac ttatataaaa
       61 aatagtaagg tttttatttc gaatccataa aatattcttg gattgcttct cgtgtttttt
      121 tcactttttc gctaagcttt tcgtttttag ccatatagaa ataaaaaatg atatcgatcg
      181 tagcaaattg ggccaaaagt gaattagttg ccgcacttcg attcgtgatt tcttttggtc
      241 tagatgtttg tacgagtaca tcggctatct tgctaagcgg gttatttcca aattgcgtca
      301 acgcaatgat ttcgatattc atgcttttcg ccagctcggc taatgcgact acatctgcac
      361 ttctacctga gtttgaaaca agccaaagca ctttcttttt ctcattgctg acaagttgcg
      421 ggagcaagac gtggatatct ttttcaaaaa tgattggctt accgacacga gaccattttt
      481 gcagaatatc ttctactact aaagaagagg cgccaacccc atataaaaaa acagtctctg
      541 ccgattccaa acactctacg actttttgga tcgtttcttc ttctagaata tctactgttt
      601 cttttatcgc tacttgtgca ttgttaccaa gtttgttttt gatagagtaa aaggactcat
      661 ttgcttctat atcactaaaa ccttcatcga ttccagtctg tagttctgtt gaaatcgtga
      721 ttttgaagtc cgtcagactt tgaaaaccga tttttttgga aaaacgtaca actgtcggcg
      781 cactcaaccc ggaagcttct gctaattcat tagctgtcat tgtagggacc ttctcaatat
      841 taccgataat gtagttggcc aacttttttt ctgctgaaga ataatcttcc atcatacttt
      901 caatacgact aattacgctc ataagttcct ccaccctttt aatgatagtc tatgtaagta
      961 taccatatca gacccttatt cacggtcgta tactggataa aaaacaaaaa aacaatccgc
     1021 ccaacagaat atcttatccc atcaatccca cagattctat tgagcatctg tgtctttgag
     1081 gaatacctgt tatctattgt tcggattgct ttctatctac tactttttta caggaaattt
     1141 acctattagt tttctctgct gtaatttttg acttctgctt ccatagcatc aataaagata
     1201 ttcaaatctt ctacagctgt ttctttactt ccataacgac gtacgttcac tgttgcatct
     1261 tccaattcct tgtctccaac aactaattgg taagggatct tttgtgtttg agaggcacgt
     1321 atcttatagc ccattttttc attacgatca tcaacttcta cacgcaaccc cttcatttgc
     1381 aaacgttctt tgatttcata agcataatcg ccatgtgcat caacagaaac agggatgatt
     1441 gttgcttgta taggtgccaa ccatgttgga aaagcgcctt tgtatacttc tgttaaataa
     1501 gcgacaaaac gttccattgt agaaacaatt ccgcggtgga taactactgg acgatgcgta
     1561 ttttccccgt cttctccaac atatgtcaaa tcaaagcgct ctggcaataa gaagtctaat
     1621 tgtatagttg ataatgtttc ttctgtacca agagctgttt tgacttgaac atctagtttt
     1681 ggtccgtaaa aggctgcctc gccttctgct tcaaagtaat ctagttctaa ttcatccata
     1741 gcggctttca gcattgcttg ggctttttcc cacattgcgt catcatcaaa atatttatct
     1801 gtattgtttg gatcgcgata gctcaagcgg aaacgataat ctgtaatatt gaagtcagca
     1861 tatactgcca ccatcaattc aagcgtacgt ttaaattcat ctttgatttg atcaggacga
     1921 acaaatgtat gaccatcatt caacgtcatt tctcgtacac gttgtaaacc tgacaaagca
     1981 cctgattttt catagcgatg catcattcct aattcagcga tacgaattgg taattcacga
     2041 taagaatgga tcgtgttttt gtagaccatc atgtgatgag ggcagttcat tgggcgaaga
     2101 acaagcattt cgccatctcc catatccatt ggcgggaaca tgtcttcttg ataatgatcc
     2161 cagtgtccag atgttttgta caactcaaca tctcccatta ttggtgtata tacatgttgg
     2221 taacctagac taacttcttt gtctacgata taacgttcga tcgtgcgtcg gattgttgcc
     2281 ccttttggaa gccagaatgg caaaccagaa cctacttctg gggaaaccat gaacaagtca
     2341 agttcttttc ctagtttacg atggtctctt tcttttgctt cttcacggag acggatatat
     2401 tctttcaaag ctttcttgtc aaaaaaggct gtcccgtata cacgttgcat catttgattg
     2461 tttgagtttc cacgccaata agcacctgcc actgataata atttaaatac ttgtatacgg
     2521 ccagtggatg gaacatgtgg tccacgacac aaatcgatga attcgccttg ttgataagca
     2581 gtaatcactt cttcttctgg caattcacta atcagttcta ctttgtacgg atcacttgca
     2641 aaaatttcta atgcttcctg ttttgaaaga acacggcgtt cgatcggcag attttctttg
     2701 acgattttca tcatttctgc ttcgattgcc ggcaaatctt ctgcagttac ttggttttgt
     2761 ccattatctg tatcgtagta aaaccctgaa tcgatagctg gtccaacgcc aaagtggata
     2821 tttggataga ggcgacgcat ggcttgggcc atcaaatgag ctgctgaatg acggactaag
     2881 ccaagtgcat cttcatggtc tggtgtgatg atctcaatac ttgcatcttc ctcgattgga
     2941 cgagtcaaat caacgagttt tccgttcact ttaccagcta aagctttttt tgctaagctt
     3001 tttgaaatgc tttcagcaat tgccaatgtt gttgttccag actcaaattc tttgactgca
     3061 ccatctggaa aagtaatttt tatcataata gtttcctcct tgaaatgttt tgattgatca
     3121 gatcatcaag ctgaaaaaca aaaaaatcct agcatctctt gaaaagagat actaggacga
     3181 aaagttcgtg gttccaccta atgttcaaac gcaaatgcgt tcctcaagcg ttgtaacggt
     3241 ccgaccgccc ttgcttcaac aagggtttcg aaagtggtca tcctttggtt ttctcctgga
     3301 aaagtttcag cctagctttt cctctcttat cgaagactct caaattcagt tggctttctc
     3361 atcaatcaat tatgtgttta tttaaccact aataaataga aaaatcaagt gggaaactaa
     3421 acttattttt tccattcccc atttacaccg tatgttcctt ttttcatctc attgatgatg
     3481 acatggatgt gttcttttgg tgcctgcgtg tttttatgaa cagcttccgt gatatctttc
     3541 atcatatttt ctaattgttc cggactgcga ccttctacta attcgacatg tacgaatggc
     3601 ataatgctct ctcctctttc tgatcgttct tttttcaact cgattctatc atactatgtt
     3661 tgtcttgggt actgaatcag gaaaaatggc tggatctttt tttgctctca aggcactcaa
     3721 aatagagacc gtatccacga cttcttgcaa aacagctccg aataatgcgg ggattagtcc
     3781 cgtacttgca attaacatca aaaaaataca aataaagatg ccaatcagta cagactgttt
     3841 cgctattctc atcgtatctc ttgaaatcac tactgctttt gctacacgca agagatcatc
     3901 tttcaagatg accgcatctg cactttcact agcagctgta gaaccatgag cccccattgc
     3961 aataccgata tctgcagcag caagtgctgg tgcgtcattc acgccgtctc ctaccatcgc
     4021 aacaggtctt tgatctttag ggatactttc taaaatggtc agtttatctt gaggcaagct
     4081 acgggcatgt acagaatcta attggacttt ttctgcaatc gtttgagcaa tggcagatcc
     4141 atccccggtg atcatcatca aatttttcac atcaagtttt cttagctcct tgattgtctt
     4201 tgctgcttct gggcgaatct gatccgaaaa taggatcgtt cctgcaaaag atcgatcaat
     4261 tgttacatag attgctgtat tggacgtttc tatttcttca gctgaaacaa aagaggcacg
     4321 tccaatcttt acttcatgat tattgactac tgcttggatt ccttgaccgg tcacttcttt
     4381 cagttgacta acaggataca gttggctgtt tttcacgttg ttcactaacg aacgagctaa
     4441 gatatggcta gattcctgtt ctgcactagc tgcatacaga agtagctctt ctttttttat
     4501 tccattgact ggctgaatcg cctctacttc caatatccct ttggtaagcg ttcctgtttt
     4561 atcaaaagca atcgtttttg tacgtgcgag cttttcaatc gttgttccag tttttacaac
     4621 gatcccgttt ttacttgaac ggctcattcc tgcaactaaa gcaatgggcg cagctagaat
     4681 cagtggacaa ggtgatgcca ctaccagtac ctcagcaaat cgtaaagggt cttttgagaa
     4741 ccaccaagaa ataccaccga tcaaataagc aatcaacgtg aaagggactg cataacgatc
     4801 agctaaacgg acaaatcggg caggcttttc ttttgattct tcgactagtt taataatcat
     4861 ttgatactgg ctgtctgcgg cacgtttttc agcgatcatt tggatcgatc cgtctccatt
     4921 taaagaccct gacataatct gatctccagg tttcttggtt tccgggcggg actcacctgt
     4981 caaagaagat tcatcgaccg aagattctcc tttggtcact cgaccatctg cgggtacaag
     5041 ttcgccaggc ttgactagta actggtcatc aatatttatt tcatcgatta ctatatcctg
     5101 caattttcct tctttgaatc gatgggcaat tcttggggtg ttatctaaca aagactgcag
     5161 ttctttaccc gctcgtttat tcgcgtaatc ctctagacta tctccgcccg taagcatgat
     5221 caatatcatc aaactagccc agtattcttt gacggctaat gttgcaagga ttgcggtaat
     5281 cgctaataca tctacgccat atcttcctga acgtaacgtc ttgatcattt ccaaaaacat
     5341 caagaaggcg agaatccctc cagtaaatgc gacaatcgca aatgcaaggg ccggctggtg
     5401 aaaaacaaat tcacttaata aagctatccc accaataagc aaagtaagta aaaatttttt
     5461 gaaatgtgtc attgtttttt ctcctctttt atgtgactta gagaggttag taataaattt
     5521 ccagctgcct ctttcatctt aagcatagtc cttgactagc actttttaaa acaataggcc
     5581 ttaattgaaa acaactatca ctatcagcgt ttccaaaatt catcagttct acaaacaaaa
     5641 atattcagca aggattactt tttcgattca tcagttaaaa agtacctttt ttcgaaaaac
     5701 tatgctatac ttttaattcg ttatttacgg cggaaggagc aaacatgcga tgaatgagaa
     5761 acaactagaa caaacgcacg tggatgaaac gatcgaacta atccagatcg aacaaaatat
     5821 gctggataca aaacaacaag aattgactca ggaaatgaat cattcgacaa aagacacagc
     5881 taatcacaag attcgagctg gttccaatga agctttttat gaatctgctg ttgaatatcg
     5941 ccaacatgag caggaattgc ttttgaaata ccatactctt gaagcacagc aaaaacgcct
     6001 gcaaacgttg tctgtcatga aagatagtcc ttattttgcc cgtatcgact ttaaagaaga
     6061 ccgtgaaaca gaaacattat atctaggtat cgcttcttta agagatctgg cagaagaaac
     6121 aatcgttatt gactggcgcg caccaattgc caatctttat tatgaaggtg agattggtcc
     6181 cgcattttat gaaactgaaa cagatagaat agatgtcgat ctgcagttga aacgacaatt
     6241 taaaatccaa gaaggacgta ttttatctat ggtagatacg tcggaagtaa tcaatgatga
     6301 ctttttgcta gagatattgg atgaatcatc aagcaaccat atgaaaaaca tcgtctctac
     6361 gatacaaaaa gcacaaaatg caatcattcg ggacacaaca agtaaagtaa tgctgattga
     6421 aggaatcgcc ggaagtggaa agacatccgc tttgcttcaa cgtattgctt ttctgttgta
     6481 tcataatcgc aagtggctcg aggctgaaaa cgttcttcta ttttcaccca accatttgtt
     6541 ttctgattac atctcgactg tattgccttc attaggtgaa agcggcgtgc ctacacagac
     6601 attcaaaaat tatttggtgc agctgctgcc agagttttct ttactagatg aacagcaaca
     6661 agaaagtggt tttttgctag gggaaaaaga tccgatacaa gtgatgaaaa gcggattgac
     6721 cttagtcgat cagattgatc gctatatcca aggcatcaca aactacggtc ccttgttccg
     6781 tgatatgaaa ataaatggcc gtacgatcct atcaaaggaa tcgattcgcc aatggtataa
     6841 agaaacaaat gaacaattac ctttacatca tagactttct ctattacaaa cgaagctcct
     6901 gaaaaaatta ggcggtctgc aaaaagacga aacacgtcag caatgggtca aagatctggc
     6961 ggaagaacag ctccaagaac tatacgcaac agatccaaat cttgaatata cagaacaaaa
     7021 ggagcgttca ttacgtaaaa aactggccaa ccaaatcgta cgtaaaagat tccgtcgaat
     7081 ccatcgaggg atcatgaatt atcaatttgt caatataacg aaacaatatc tccactttct
     7141 acaaactgtg ccactttctc ttctaacacg acataaaatc agcgagacca ggtggcagga
     7201 acatttgaca actgttcgta cacacttgcg tcaaagagaa ttgcgtcaag aggatgctgt
     7261 actattcttt ttactgatgc gccgaacata tccagtgacg gttacacaca aagctcgcta
     7321 tatctttatt gatgaaatgc aggatttcgc accatcacaa gtggcattgc tgcaagcctt
     7381 gttcccaaaa gcaaatttga ctttctgtgg agatctaaat caaaatgtct ttggaaatga
     7441 aacgatcgtt ggatcattgg aagacctttt cgtggaccgt gaagtcactc gtttccaatt
     7501 aacgacaagc tatcgctcca caaaagaaat cactgatttt gctaatcatt ttattgtgaa
     7561 agaacatcaa gtagaaacaa cagcaagaaa aggagacctt cctttgctca tccaaggaac
     7621 aacagatgat atcaaattga attggttagc agataccatt cgaacgactc agggaaaagc
     7681 ccggtattgg cgtacagcaa tcatagctaa aacacaagaa gaatgcgaag ctctttatgc
     7741 tcaactgcca gacgagttga aacaacaagt ccagctgatt ttaaatgaga gtgactttat
     7801 gaaacgttcc attattttac ttccagcctt tttagcaaaa ggtctggagt ttgaccgagt
     7861 tttcttgtgg gatgtagatg cagaacattt ttcttctcag caagacaaat tgatccttta
     7921 caccatgtgt acacgtgcga tgcatgaatt gacccttttt acctccaata atgaaagtcc
     7981 tttgttaaac aatatggata tttcatctta tgagaaaaaa gtactgtaag taaaaagaca
     8041 actgctatca tcaatgttag cagttgtctt tttatttaca ataataattg cagaaaaagt
     8101 aattcaatca tttaaggtca tttattctat atataaatcc aaatctgttt caggatattt
     8161 taatttgaca agcgaaataa tcattttcag ttcttccatg ttttctggat attctgggtc
     8221 aaataccttg ctatgacggc caatcccgcc attgtcgatc aatcggtaaa ttcgattcca
     8281 ttcaatgaat tggtcaattg attggaaatt gctgattgga tagccgccac aatccaaaat
     8341 ctctgataca cgaaatgcta ggtatgcact atcgatcaca ttattttttt tcgtgtctat
     8401 cttctcattt ctttcgataa gaattttttc aggacgatgc ttcaagctcc actctaatag
     8461 tagaaagaaa gcctccatcc gatactcttc ttcaaataac cgaaaggctg aatcagaacg
     8521 acgaaaaagc agcggaatca gataattttg tctataaatc agcattggtg cgcggtccac
     8581 catacactct aaataacggt tcagataccc tgtaccgaca tagtttccta atcgatagcc
     8641 ttcaacgccg aatcttgcgt atttttgcgc taattttgaa caatcagtta cgttcacagc
     8701 taactcttct actttgcggt aaccgcttaa gatacgttca aaaggttcta aagtgatttt
     8761 tgcttccata ttcttccccg tccatcttcc acatacttca tatcatttca catgatactt
     8821 atgctctcat cataccacag gttgaattag aaaagaaaat aaatcacaca aaaaagcaag
     8881 acaatctgtt agtgagattg tcttgctttt cgaaaaagac tgaatacttt tcgttaaaac
     8941 tggttgctta gtcttctaat gccacattat ggaacacatg ctgtacatct tctagatctt
     9001 ccagtgcatc aatcattttt tcaaatgttt cttgatcttc tggagatagt gttacctcgt
     9061 tttgagggat catttggatc tcagcgattg aaaaatcagt gattcctttt tcttttaatg
     9121 cttcttgtat actatggaaa tccgctactt caccgtaaac gatgatttgt ccttcttctt
     9181 ccattacatc gcgaacatcg atgtcttttt ccattaggta ttcaaggatc tcgtcagcgt
     9241 cttctcctgc aaaagcaaag attgctgtat tgtcaaacat gtaagaaacc gctccggaaa
     9301 cacccatgtt cccgccattt ttcccaaatg ctgcccgtac gtctgctgct gttcgattca
     9361 cattgttcgt caatgtatca acaatcacca ttgagccgct tggtccaaat ccttcatagc
     9421 gcaattcaga gtatgtttca tctcctgcac cttttgcttt ttcgattgct cggtcaataa
     9481 tatgttttgg tacattataa gttttagcgc gatcaatgac gaaacgtagt ttttggtttg
     9541 catgcggatc aggatcacct gattttgctg ccgcgtagat ttcaatccca aattttgcat
     9601 aaatccggct attattagca tctttagcag ttttctttgc tacgatattt gcccatttac
     9661 gtcccatatt ttcacttcgc tttctgattt gatagtttca taacgcaaaa acgtcctgtt
     9721 ccattatact tgtgaatcac gattctgtga agcttttttt ctttcttcct tcattcttct
     9781 catttttagt caattatcta tactcggatt gggatttgcc cgaaattctg ttccggaata
     9841 ttgcctccca attgatagat ttgatcactt ctaactaaca gttccgcttc ttgtgcctct
     9901 ttttttccga ttcgggaata aatagaaatc tctttttttt cttttaagaa tcttcttcca
     9961 acatcggtaa atccaagtag tcgaagataa tttttatttt gttggacagg aatctcttgc
    10021 tttttgatat tcagcaagac ataacaagat aatcgttgga tccgtgtcca tgtatatctt
    10081 tttgttttta cctttgacac aaaatcctca aaactatcag cagttgcagc tgctgttttc
    10141 atgcgcgatt ctattccttc ggtcatctga tagatatctg tcagttccag caaagtagaa
    10201 gataacaacc gataacgcaa atacggccaa taagattccc aagtctgcag cgaataagtt
    10261 gaaagatctt cttctgtctg aggcggcaca agtccagcta ttctttttcc ttcttctaac
    10321 gcatggcgaa tggctgttgc actggctatc ctctgatctg tgatgatttt ttcatgatag
    10381 cctgcacttt tacgtcggat cggatgtaat tccatagggt ggacatactt ggcattttcc
    10441 tttgcataac ttaatcccaa gatatgattt ggcgaagaaa aatcaagcgt aatatcggga
    10501 tacagactgc gaaaaacctc agtcattttt tgcggataac tatacgtttg atcagacagt
    10561 tcttgaaatg cctcttctat cctatcttga ttctcttgga caaagcgtcc gaaactgctg
    10621 taatcaaacg aagtctcttc ttctgttcca aaacacaaac tgtcacagtc cagtgtttgc
    10681 aagattttta ctgcaccttt agcaaaatag tcagctgact gaaccgccca ttcgacggga
    10741 agctccacta ctaaatccac accattttgt aaagcttcat tcgcgcgatg ccatttatcg
    10801 atgatagccg gttcgcctcg ttgcaaaaaa ttcccgctca ttacagcaat gacgacctct
    10861 gctcctgttg tttttcgtgc catttccaca tgatagcgat gtccattgtg aaaaggattg
    10921 tactctacga taatcccgca gcttttcatg cgcatcctcc ttcatagtta tcgataaaaa
    10981 agaaatagag acaagtaaaa aaaggcaaaa atcggaaaaa atacccgaac tatataaact
    11041 attctcgcaa acacaatact tctgcgacga aaagctgcac tgcaagagca atgcctatat
    11101 cgttcggata tataatttat gttgaagatt tattcttttc ttgttaactc gagcaggcaa
    11161 aaccgctagt ctttgtatgc tacaaagaac cagcgctttg atttctctga tgctgcttcg
    11221 tctgtaaaat cagaaaatgc ttcgactttc gaaaagccgc tgttttccag cattcttaaa
    11281 taactttcca atggataggt acgctcttga tgtagttcat cttcacgaat aaatcgacca
    11341 gacccttctt gttcttcaac gaaaaaggta agaaaatgtt cgatgctgtg tttctcgctt
    11401 cctggataac tatcccacaa aaaagcaaaa ttatcccctt gataatgata gctatattct
    11461 ggaaaaattt catctacttg ataggtggag tgtacgtcaa ataaaaatcg gccattttct
    11521 tctaacgctt gatagacttc atcaaacact tgctgtacct cttgtctatt tttcatatag
    11581 cacagcgaat cagaaaaaca ggtaattgcc tgatattgtc cgatttctga taaatcgagc
    11641 atattgcctt ccacaaattg gatcgatgcg tcttcttcat atgctcgctg gctggcaatc
    11701 atcaacatct cttctgaaag gtctaaccct gtcactttat atccatcttt tgcaaaggca
    11761 actgctaatg ctccagtacc gcatgccagt tccaaaatat tttttgtgtc ttcttgtaaa
    11821 tgacgtttcg aaaaagctag ccattgctga taaagtgttt cgtccatcac ttcatcgtaa
    11881 atgaaagcaa atgtttcata agccatatgt taatccaacc attcactaag atcgactagc
    11941 ggagcatctg accatagttt ttccagatta tagaaacttc gttctggtgc atggaaaaca
    12001 tgtacgatca catctccaag atcgatcaat acccattttc cgccttcttt tccttcaata
    12061 cgttttactt catatttgtt ttcttcttct ttatcaatga tttcttctgt aattgcgttg
    12121 atttgacgtt cgctatttgc tgaacagatc atgaaataat ctgccaacaa ggaaacattt
    12181 cgaatgtcta aagcgataat gtcttctgcc cgttttgaat ctgctgcttc aaccgcaatt
    12241 tttaattgtt ctttactatc tatgatgatt tcctcctaat atgagcagct tatttttttg
    12301 ctacccagta attatatgtt tcgattgttt ttggataaat ctgttgttct tgcgcaagca
    12361 aatgggccaa tgtatgtttt gtttcaaatg ctacagcttc gtctagatca gcccatgcaa
    12421 tggctcgtgc atcttgtacc ccaggaaaat ctcgacctgg ttcaatatag tctgctacat
    12481 aaatgatttt gtcaaggaga ctcatcttag cagccccagt cgtatgcacc cggattgcat
    12541 gaagaatttc tacatcggtg attcctaatt cccgctctac aaacgaagcg ccaacaagac
    12601 catgccagat tgcattatta taattcaata actctggatc aaaaccgtct cttacgatta
    12661 ccattttgaa ctcctcatct ggtcgttctt ttgcataatc atgtgtcaat gcagcaatac
    12721 tagctttttc aggagaagca ccgtattttt tggcaagagc cacggctgtt tcttctactc
    12781 caagcacatg cttgaagcgt tgttcgctca ttctcatctg tattttttgc atcaggtttt
    12841 ctctagaata agcagtatat tcattactat agtccatatt catacaaccc ttcattttga
    12901 atataatcaa ttactttatc tggaacaagg tagcggatag aacagccttc tttgatcttt
    12961 tgacgtattt tcgtggaact gatgtcgatc tcaggtacat ctacccaaat tacaggatat
    13021 ggagtatccg ttgtgtatcc tggtcgacgt atacccacaa agttgaccat agaagtcagc
    13081 tcatcgattt tataccattt tggcaaatat tctaccatat ccccaccgat aataaagtaa
    13141 taatctgtat ccggattgtt ttgcgtcaat tccttcatag tatcataggt gtaacttttc
    13201 ccaccacgag ctaattcaat cgtttcaatc tgcaaaaaag gattgtcttc cacagctaat
    13261 tccaacatgt tgagtcgatg ttttgcatca atcgtctgtt tctcatcaac atgaggtggc
    13321 tgataagaag gcatcaaaaa tacgcggtca agacctaaat tccggccagc ttgttcagcc
    13381 atcactaaat gtgcaaggtg aacgggattg aatgttccgc ccaagatccc tacttgcttt
    13441 ttcttctcta agaattgagg catttcttca ggaaataatt gtgaacgaac aaatgtctta
    13501 gcttgcgtat tcatcatggt cactccttat attgctttta cttcttttga tagcctttga
    13561 tacttttctt ttgtggaagg tttgtataat acaataacac gtccaatgat ttggacgatt
    13621 tcgcaatcta tttctttttc taatacttga gctgcttctt ctgcaatctc atctgtattt
    13681 tgaagtaacg atactttgat cagttctctt ttttctaaag cttcttcaat ttgaacgatc
    13741 attgctgaat tgatcccacc ttttccgatt tgaaaaatcg gctgaagatg atgcgctttg
    13801 ctacgtaaaa aacgcttttg ttttcctctt aaactcataa ttttccttct tccttaaatc
    13861 aatgctttgc gtttcaatac atctacaccc tttggcgccc agcccgcaat tactactggc
    13921 gctgttacag tgatccagcc tagaccggca aaaacgatgt ctgttttttc tttcacagaa
    13981 aactcaaaac gaacaagctc cggaaatgcc tcgacttcat caggacgagg aggctgtaat
    14041 aatccaccta catgtttttg ataaaattcg tctgcttttt ccagtttcgt tcgatgaata
    14101 tttacatcat ttgacacata agcaataaat gaagcacgat tgccgctgat aaaatcaaat
    14161 cttgcaagtc cgcccaagaa aagtgtttgc ccttcattca gctgataaac tttaggtttg
    14221 atttctttta acggtgcaat gatccgcaaa tcttttttac ctaagtagtg cgccatttga
    14281 tggcgatgga taattcccgg tgtatcaatc aaaaaatgcc cgtcatctaa aggaatctcg
    14341 attttatcca aggtcgttcc tggaaactgc gaagttgtga ttacatcttg tactcccgct
    14401 gtatttttga tgatttgatt gataagcgtc gattttccga catttgttac tccgacgata
    14461 tagacatccc gtccatcgcg gtattcttcg attttttcta atagttccgt catctcatga
    14521 gatttcttag cactagttaa taatacatct actggacgaa ttcctgcttc gtaagcacgt
    14581 tccttcatcc attgtgttaa tttagttttt ttcaatgatt taggtaatat atccactttg
    14641 ttcccaacta aaagtactgg attgtcaccg acaaaacggt gtagtcctgg gattagtgaa
    14701 ccattgaagt caaaaatatc caccacattg acgatcaatg catctttctg ccctaattca
    14761 ttcagtaagc gcaaaaagtc gtcatccgtc aatgagacat cttggatctc attataatgg
    14821 cgtaatctga aacaacgctg acaatagact tccccggaag ctaaaccttt ttccaaagcc
    14881 gtttgcggcg tgtatcctaa ttcaccgggt ttgtttgttt gtataacggc accacagccg
    14941 atacattgta attcttctgt cattcgattc cacctcgcca gcccatatca gggtgttttg
    15001 ctaatagata ggccataatt cttttttccc aaaaacgatt aaattgtgtc ttccaactat
    15061 ctgttgcgat aatgggtttt acaaggatgc tacgtatccc tgcacgattg gctccacgta
    15121 tatcagtcat aatctgatcg cctaccatta ctatctcatc atctgctaaa tccaattgtt
    15181 tctttgctat gttgatccct cttgctaaag gtttcattgc tcgtgcaaca tacaaaagat
    15241 caaatttttc aatcgctcgc gcgacacgtt ttgaattatt gtttgaaaca acaacaacag
    15301 ggattcctgc atttttcatt tctaaaatcc aagtaagcaa ctcttctgtt ccatctggat
    15361 tattccatgc aatcaacgta ttgtctaaat cggtcaatac tgctttgatg cctagattct
    15421 ttaattgggc aggtgtaatc tgatagatgg cttctaccat ccatgttggt ttgtaatttg
    15481 aaaacatgct gtctcctcat ctgcaaaaaa tgaaggagca cataaagtac tcctcaaccc
    15541 cgtattatac tgcatatcag caaaaaaaac tacctctata ctcgttcgtc tttcagaaaa
    15601 agcatttctt tacaaaatga ccaaaaataa agattgatca cagaaaaatt tagaaacaaa
    15661 aaacaactaa gcgagcaaga ccattcttta ctctttgctt agttgttaaa cacctaatat
    15721 tggcggaacc tcttgtatct tttttccagt aattcatgct ggtcgaatcg aacaagttcc
    15781 gtcaatcttt caatcaacgc ttttttcata atgtgtgcga ctttatatcg tggcaactgc
    15841 tcgccgttga acgtttcagg aataactcga tcaacgattc ctaactcgcg tagttctggc
    15901 gcagtaattt tcatcaactc agctgcttct gcggcgcgac taccgtcttt ccacaaaatc
    15961 gacgcaaatc cttctggaga aagcacagca taaatcgttt cttccagcat ccagacttca
    16021 tccgctaaac ctagagcaag ggcaccgcca cttccacctt caccaatgat gatggcgatg
    16081 atcggtacct ttaaatcaga catttcgagc agatttctgg caatagcctc tccttcgccg
    16141 cgctcttctg ctcctacacc gcaatatgca cctgctgtat tgatcaatgt aatcaccggt
    16201 ctattgaact tttccgcttg tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg
    16261 ggagaaccaa aatttctgcg gatattttcc tgcagatccc gtcctttttg aataccgatc
    16321 actgttaccg gtttgccatc taatcgagcg attccaccaa caatcgcttc gtcatctgca
    16381 aacaaacgat caccatggca ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag
    16441 cttgtccatc gttctttagc tcttgctaac tgaacgacat catgtgctgt cttatccacg
    16501 atctatccat cctttctgtg tatgcaattc aagaagcgta tggattttct gcttcaattc
    16561 catgcgggga acaatctgat cgacaaaacc atgagataat aagaattcag ccttttggaa
    16621 atcttctgga agttcctgct tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat
    16681 caagctttgg ggttctgcta gaatgatatc gccttccatt gcaaaactgg cagttactcc
    16741 tccggtcgtc gggtccgtta aaacagttag ataaaaaaga ccttcattgc tatgccgttt
    16801 gacagcagca gagatttttg ccatctgcat tagagaaaag attccttctt gcatccgtgc
    16861 gccacctgaa gctgtgaaca agacgactgg aagactttcc tttgttgctc gttcgaataa
    16921 tctggtgatc ttttctccga caatcgttcc catacttccc atgataaaat taggatccat
    16981 cactccaata atgaagggaa tcccttgaat ggttgcttta ccagtgagga ctgcttcatg
    17041 aagtcctgtt ttatcttgca tttttttaat tttttccgta tattcgggaa atgctagcgg
    17101 atctttggtt acaagatccg tatcccattc ttcaaaactt ttttcatcaa taatcaaagc
    17161 tagacgctgc catgcaccga ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc
    17221 accgatgtct ttcgtataaa ggatatgttt gcaatttgga cactttgccc acatgttatc
    17281 aggaacagat ggcgagttag caggtttgct ctctctatta ggattgatcc gaatatattt
    17341 ctttttctta aacagagcca tctatgcgcc tcctatctcc gtttctggtg tccaattcgg
    17401 taaaaattct tcttgtaaaa atgctgtgct gtaatcacct gctatgactg cagggtgact
    17461 aatcaaatcc atctgaaatt ctgcgttcgt gatgattcct tctgtgataa attcacttaa
    17521 ggctcgctgc atcttcatta atgcttcaaa acgcgtattt ccatggacga ttatttttgc
    17581 aatcattgaa tcataatatg gcgggatcgt atatcccggg taaactgcac tgtctacccg
    17641 cagtcccatc ccacctgctg gaagcagtaa gttttggatc ttccctggag aaggagcaaa
    17701 atggaaagca ggattttctg cattaatccg gcattcgatt gcgtgtcctt ggaatactac
    17761 ttcttcttga cacacattca aaggctcacc ggctgctatc tccacctgtt tttttactaa
    17821 atcgattcct gtgaccattt ctgttactgg atgttccact tggattcgag tattcatttc
    17881 catgaaataa aaatcgcctt cttcatccat cagaaactcg atcgttccgg cattttcata
    17941 attgactgct ttagctgctc gaacagcagc gtctcctagt gcttctcttt ttgtttgtga
    18001 gatcactaca gaaggagatt cttccagtac tttttgatta ttacgttgca aagaacaatc
    18061 tcgttctcct aaatgaatga cattgccaaa atgatcacct aaaatctgaa cttctatatg
    18121 gcgagcagga taaatgattt tttccaaata catatcatca tttccaaatg ccgctgctgc
    18181 ttcttgctga gctgaagaaa aatgctgtgg gagctcttct tttgattgaa ctttccgaat
    18241 gcctttcccg ccaccacctg cagctgcttt cagcatcact ggataaccaa ttctgtctgc
    18301 tatttccaat gcttcttcta ctgtagataa tacaccagta cttcctggaa tgactgggac
    18361 atttgcttct tgcatcaatt gacgcgcatt gatcttattt cccattgcat cgatcgttgc
    18421 acttttagga ccaataaatg tgatattgca ctcttcacac atggaggcaa atcggctatt
    18481 ttcagataaa aaaccaaatc ctggatgaat tgcttcggct tttgtcacga ttgcagcgct
    18541 cagtacttcc tgaacattca gatatgaatc aacggctttt gccgggccaa tgcagattgc
    18601 ttcatctgct aactgtgtat gaagtgcttc ggcatctgct tctgaatata tcgctacggt
    18661 ttggatccct agttcgcggc atgcacgaat gatacgtaca gcgatttcac cgcggttagc
    18721 tatcaatact ttagaaaaca tatttcacct atccaatcat aaaggttaat tccgcttcgg
    18781 ctactttttt accgtctact ttagcgattc ctttgcctat tccggctgaa gctttcactt
    18841 tcaatatctc aacttcaagg tataaggtat ctcccggtgt aactttctta cggaattttg
    18901 ctttgtccaa tccgccgaaa taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg
    18961 caactgctcc tgcttgagcc atcgcttcaa caattaatac ccctggcata acaggttctt
    19021 ctggaaaatg tccttgaaaa aagggctcat tgatcgttac atttttctta gcaacgatcc
    19081 gttctccttc aaccatttct tccactcgat caatcaaaag cattggataa cgatgtggaa
    19141 tgatttcttt tatttcttgt atgttcattg atttccctct ttcctgcttt gcttattttt
    19201 gctgaatcct aaagagtggc tgtccatatt cgacaactga ctcgttttct acaaggattt
    19261 ctgtgatcac tccgtcggcc tcactagtta tctcattcat cagtttcatc gcttcaataa
    19321 tgcagataac ttctcctttg gcaacttgat cacctacttt tttgaaagct ggtttgtcgg
    19381 gcgccggttg cagataaacg attcccacga ttggcgaaac aacctcgatt ccatcaaata
    19441 ctgccgattc tttttctttc attgtttctg gagcagttgt tgcttgttca ggtatgacat
    19501 tgactggctc cgaaactggc aacgtttcgt ttgtttgaac catattcatg cctcgacttg
    19561 ttttattttt attcatatac aattcaaatt gaccttcacg caaatcgaat tccgttagcg
    19621 aggattgatc aaattgactg actaattctc tgatttcatt gatatccaaa attattcctc
    19681 ccagcgtttc atgcacagaa ccgcattatg tccgccaaac cctaatgaat tactcattgc
    19741 ataagtaaaa cttgcaggtt tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc
    19801 tcgtttttct acattgatcg taggtgggat aaattgatgt tctaatgcca gcaaagttgc
    19861 caaggcttcg atcccgccgg ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct
    19921 gacgtaagta tctctgaaat tttcacctaa tgcgatttgg attgcttttg cttctgcctg
    19981 gtcattggct ggtgtacttg ttccgtgagc attgatatat ccgacttgtt ccggctgtac
    20041 accagcttct tccatcgcaa gcttcattgc tttagacgca ccagaaccat ctggagtagg
    20101 agctgtcata tggtaagcgt cacagtttgc accgtatcca atgatttctc catagatttt
    20161 ggcaccgcga gcttgtgcat gttctaaaga ttcaagaact agaacacctg ctccttcacc
    20221 catcacaaaa ccattccgat tttcgtcaaa cggaatcgaa ccacgattag gatcagttga
    20281 ttctgagagt gctgttaaag ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc
    20341 tgttcctcca gcaagaatga catcagaata gccatgtttg atgttacgga atgcttcccc
    20401 aattgaattc gttccgctag cacaagctgt tacgatagct gtacaaatac cttttgcacc
    20461 tacgcgcaaa gcaatatttc cagctgccat gttagcaatc gccattggta cgaacattgg
    20521 agaaactcgt tttgcacctt tttcatgtag acgaataact tggttttcga ttgttggaag
    20581 accaccgatc ccagatccga tgatgacgcc aaaccggtct tgattcattt tttctttatc
    20641 taaaccgctc atctccattg cttctaaagc agcgtagatt ccatagatcg aaaacatatc
    20701 cattcgtttg ctatcttttt taataaaata tttatcgtaa gggaaatctt tgacttcacc
    20761 cgctaaggta atccctgttt ctgaagcatc aaatttagtg atcggtgcga tgccattcgt
    20821 cccctctttt aagctattca aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc
    20881 atagccagta attaccactc gattcatttg tttgctctcc ttttcttatc cgtgcatgac
    20941 caatccgcca tcgacattga tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac
    21001 tgctgtttgt gcaatatctt ctacttgtcc gaaacgctgc atcggaattt gcttttccgc
    21061 ttgttccttg actttgtctg ctaaaacttc cgtcatatca gttgtgatga atcctggcgc
    21121 aatggcatta catgtaatgc ctctagttgc agcttctcgt gccacagatt ttgttaatcc
    21181 tactacgcca gctttactag ctgcataatt tgcctgacca atatttccaa tcaacccaga
    21241 tacactagag agattaatga tggccccttc tcgctgcttc atcatttttt tcaatacgtg
    21301 ctgcgtcata ttgaaggttc caatcaaatt gatatcaaga cattttttga aatcttctgc
    21361 atccattcgc atcacaagtt tgtcatttgt gattccggca ttgtttacta acacatggat
    21421 cgatccaagc ttttcttctg cttctttgat catttgtccc gctttctcat aatcagagat
    21481 gtcaccggaa actcctacgc attttacacc gaatgcttcg atttcttcta tcttttcttt
    21541 tggaatctct ccgcgaccat tcaaaatgat atttgctcca gcttttgcaa aagctaaggc
    21601 catcgctttg ccgattcctc ttgtactacc tgtgataaat acattttttc cttttacatc
    21661 cataacgttc ctccttatag tattgctatt gtttcatcaa atgtctgctt attctcaaca
    21721 cgcagaactg gtattgtttt atcgattttt ttcataaatc cgcttaatac ttttccgggg
    21781 ccgacttcga tgacttgttc gatgccgatt gtttttaact tatggatact ctcatagaaa
    21841 cgaacaggtt gcatgacttg ctgttcaagt agtcccgcga ttgtttcttt cttcattatt
    21901 tccgttgttg tgttgcttat gataggaaaa gaaggttcag agaattgaat ctgacttagg
    21961 tccttcgcca gtttttttgc tgccggttct aaaatagctg tatgaaaagg cccgctgaca
    22021 tttagaggga tcattctttt gaaccctttt tcttttaata gtaaaacagc ttcgtctact
    22081 gctttttctt cgccaccaat cacgatctgc tgaggtgtat tataatttgc aggagatacg
    22141 atgccgtact tgctagcttc atgacagctt tcctcgatcg tttcaatcgg cgcattcatc
    22201 actgcaacca ttttcccgct tccagctggg gcagcttctg tcatataagc gcctcttttt
    22261 gctactaacg caactgcttc agtgaaggat agcgcattac tagctactaa tgccgaatat
    22321 tctcctaaac tcagtcctaa agctgcatct gggatgattc catgctcctt caataagcga
    22381 taatacgcga tgctgactgt tagaatagct ggctgtgtat attgcgtctg atccaaacgt
    22441 tcattttctg taaagcaaag ttcagccata tcgtagccga gaatctcact tgcttcatca
    22501 aatgtctgct ttacaacggc ttcttcatat aaatcttttc ccataccttg atattgtgca
    22561 ccttggccac taaataagaa tgctgttttc atgtttttct ccaatatatc cagaatatat
    22621 gtttgattct tcctattcaa aaaggccgag acattcctat ttcaggaaat gtcccagccc
    22681 ccaacctaat tttaatgatt atgcagtttg tttttctacg taagtcacta aatcttgaac
    22741 cgttttgatt ccgtcttctg tttcgatttt cacgtcaaat tcatcttcaa tttcattgat
    22801 gatttggaat aaatccaagc tgtcagcttc taagtcttct tggatgtcag tagtcaattt
    22861 gatttcttca gtttcttttc ctaattcttc tgcgatgatt tcttgaattt tttcgaatac
    22921 cataataata tattcctccg atattgtttg ctttttattt ttataattta acagtctaca
    22981 actccatgag aatacttccc catgttaaac ctccgccata gcctgtaaat attactttct
    23041 gctgttttcc caaggtgatc tttccactag aaactgcttc atccaataag atagggatgc
    23101 ttgcagcaga tgtattccca tatttatcca tattggtcag gaacttttct tgcggcatct
    23161 ttacttttcg ggcaattttc tcaataattc tgaggtttgc ctgatgcaaa agcagataat
    23221 caactggttt gtctttcatg acttcttgga tattgtttgc aacgtctcgt acggcaaaat
    23281 caaaaatatc tctgccagtc atttgcaaat aatacgaacc ttctgaatct tctgaataga
    23341 acggattttt attttgctgg tagccagcgg tcaatgagct acttctggta ccgtcagctg
    23401 ctaatttttc tttcagcaat tttttctgtg gattcgcttc taagagaatc ccacctgctc
    23461 catcgccaaa caacacagca gtcgtacgat cagaccaatc gattagctta gatagtgttt
    23521 caccaccaat cactagtccc ttagttgagc cgcagcggat cattttttca gccatgctta
    23581 acgcataaac aaatcctgag cacgcagcac tgatatcaaa agcgatagca tgagttgctc
    23641 ccaatttccc ttgtacttgt gctgctacag atggcgtcgt gtaatcgggc gacattgttg
    23701 ctactaaaat aaaatccaaa tctttagggt ttagattttt ctgttttatc aatttttccg
    23761 ccactaaaat gcataaatca gaagtctcct gatttgttac acatcttcgt tcacgaatac
    23821 ccgtccttga cgaaatccat tcatcagagg tatccatcat cttggaaaga tcattattag
    23881 taataatctt ttcaggaagg acgctggctg ttgcagtaat cattccaaat tgttccatta
    23941 aatatcctca cttatagtct tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg
    24001 catgctgttc ctccgtagcc attccgtcta agatgttctt gaccatttca cgatgaaaat
    24061 gctgatggac ccgaaatagt aactttcctt ttttcgttag tcccaacttt acaacgcggc
    24121 ggtcatcttc actgcggata cgttcgacat agcctttttt tactaacttg ttaatagcag
    24181 tggtcaaggt cccaacagta attgatagtt ctttggctac ttctgaagtt gtttttttct
    24241 tgtacatccc gattgcctcg atcgtgtgca tctctgtgat tgacagatct ttgaattgag
    24301 attttttcag ctcagactct tcgattgtca aaatatcatt gaagacacta acaagataat
    24361 catttactgt ttttaaatct ggttccatgt tccaccgtcc actaattagt ttgattatca
    24421 aatcttttga tgttcaaact atatcttaac ttttttcaaa atgcaacctt aaaagatcat
    24481 ttttttacta tatgagcttt ttttaagatt ttactcattt aaaacaactg ataaatcagc
    24541 atttataatt tcttttatta gaaaaatcat ctatctcaca tattttgatt atcaaagtat
    24601 tttctgtgaa cagaactata gaattcctta aaacttgtcc attatttcaa aatttgttat
    24661 agtcttttta ttcaaagcgt aaaaggagat cctatgttaa aagaaaaaat gaaaactctc
    24721 cccatgctgc cgggtgtcta tcttatgaaa gaccgtgatg gtaaggtcat ctatgttggt
    24781 aaagcaaaac ggctcaaaaa tagagtaagc agctattttc atcagaataa acagcattca
    24841 aaaaaagtat tacgtatgat tcatcatata acagattttg atttcgtcgt agtagacacg
    24901 gaattagatg ctttattgct cgagtgtcaa ttgatccaac attaccgtcc tttttataac
    24961 cgacaaatga attatttcag taattataat tatgtacata ttaccaataa gggatttgta
    25021 ttaacagata ctcctacggc tcgaacatac gggccttttc gtctctataa aaaaatgccg
    25081 tctattttgc gtataatgga agaaacttat caaatgcctt ggttgtctga aattagtttg
    25141 ttagctttgc gagcccagct tcctgaccta caggaaatga cttttgaaca gaaaaaaaag
    25201 gaactccaag ggctgtttca aggccaaaat aaaaagctac taacttactt gaaaaaaaga
    25261 caacaacatt tcatctatca gttgaatttc gaaaaagccg gtatgcttca aaaagatatc
    25321 gaattagtta cttattttat tagacggatc caggaacaaa agcatttttt aagaacacca
    25381 tcgctcacct tcagcatgcc attagcagca gatgagagtc agaaaaagca ctacctcgtt
    25441 tgttatggtc aacttgcaga aacaatgatt gcctctgctg acgtttctcc tgacttctat
    25501 tatgaaaaga aagaagctca tttatctttg aaacgtcaat tgtcaaaaga agaaatcgat
    25561 cctgtacaaa tcttgatcag ttaccgcaaa aaattagaaa aagaacagtc tgaaattgaa
    25621 ctgttaaaca aa
//
LOCUS       DACDNO010000041        24965 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDNO010000041 DACDNO010000000
VERSION     DACDNO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.041"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(8..2389)
                     /locus_tag="IWI22_001750"
     CDS             complement(8..2389)
                     /locus_tag="IWI22_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9169142.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="IWI22_001751"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="IWI22_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9169143.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="IWI22_001752"
     CDS             3090..3629
                     /locus_tag="IWI22_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9169144.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWDEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="IWI22_001753"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="IWI22_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9169145.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="IWI22_001754"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWI22_001754"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="IWI22_001755"
     CDS             4736..5902
                     /locus_tag="IWI22_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9169146.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="IWI22_001756"
     CDS             5948..7444
                     /locus_tag="IWI22_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9169147.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMEDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="IWI22_001757"
     CDS             7525..8208
                     /locus_tag="IWI22_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9169148.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="IWI22_001758"
     CDS             8853..10043
                     /locus_tag="IWI22_001758"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9169149.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKRM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLVTYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKAGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="IWI22_001759"
     CDS             10145..11098
                     /locus_tag="IWI22_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9169150.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWDLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDAMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="IWI22_001760"
     CDS             11167..12516
                     /locus_tag="IWI22_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9169151.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETRGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12698..13021)
                     /locus_tag="IWI22_001761"
     CDS             complement(12698..13021)
                     /locus_tag="IWI22_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169152.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPQALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13150..14313
                     /locus_tag="IWI22_001762"
     CDS             13150..14313
                     /locus_tag="IWI22_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169153.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKLAFDSGTVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14383..15732
                     /locus_tag="IWI22_001763"
     CDS             14383..15732
                     /locus_tag="IWI22_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9169154.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15777..16208)
                     /locus_tag="IWI22_001764"
     CDS             complement(15777..16208)
                     /locus_tag="IWI22_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9169155.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMEKSLA"
     gene            16596..19238
                     /gene="alaS"
                     /locus_tag="IWI22_001765"
     CDS             16596..19238
                     /gene="alaS"
                     /locus_tag="IWI22_001765"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9169156.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDAPTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19438..20034
                     /locus_tag="IWI22_001766"
     CDS             19438..20034
                     /locus_tag="IWI22_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9169157.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20156..21304
                     /locus_tag="IWI22_001767"
     CDS             20156..21304
                     /locus_tag="IWI22_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9169158.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFDDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21342..21755)
                     /locus_tag="IWI22_001768"
     CDS             complement(21342..21755)
                     /locus_tag="IWI22_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9169159.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21834..22265
                     /locus_tag="IWI22_001769"
     CDS             21834..22265
                     /locus_tag="IWI22_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9169160.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22364..22597)
                     /locus_tag="IWI22_001770"
     CDS             complement(22364..22597)
                     /locus_tag="IWI22_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9169161.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22779..24449
                     /locus_tag="IWI22_001771"
     CDS             22779..24449
                     /locus_tag="IWI22_001771"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9169162.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGIIAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNEWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24614..>24965
                     /locus_tag="IWI22_001772"
     CDS             24614..>24965
                     /locus_tag="IWI22_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9169163.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRE"
BASE COUNT         8015 a         4395 c         5268 g         7287 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagggt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactggg
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaaac
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atccttgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgga agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaatttac ttctggaaca
     8281 ctgtttacca agaggtcatg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcatgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaata
     8521 aagatggaag ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atggcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaag gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg gtcacctacg
     9481 ttgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagcaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acgactacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg ggatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgcgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aagagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgc tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttatccc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgcc
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcactct ctgccaaaca aaatatttta tttaaatgag gagtacgtgg aagaaatgtc
    12601 aaaagtactc gttaattttg atcacgtaaa tgtcgttttg tctacaatga aatagcaaag
    12661 taaaagacag aaatcgggct caaccgattt ctgcctttta ctttgatttg cgtaactgaa
    12721 ctattcggcg tacgccgcga taaatattta ataaaaccaa gcaaccagaa atgaaaatgg
    12781 gaagccaaat cagatagaca gggtagccaa gcactttaga caaagcttgt ggagtcattt
    12841 tatccatttc aagaagtgct tgctgccaag tctgaagatt gaagactagc atgaaacccg
    12901 aaataatcag gagaataaat cctaaataat gaattgtagt tctttttctt acataagcga
    12961 tcgtagcata aaggaacagt aaaaaaatag gtacgccaaa aacaaagtac atattcagca
    13021 tctcgacacc ccctttgtgt ttatcttact ttactttgaa agaaatgaga aataatagcc
    13081 tcgttacttg cttgaaacag caaaggactg ctaagataag agagatacaa agaatcaagg
    13141 aggagtcata tggcaagtta tatcgaaccg attcgttttg cgctgattgt ttttccattt
    13201 ttagccttag cgatctcatc tgtttttttt atttatgaat atcgaaagta tggaacattt
    13261 ctagtaacaa gggcagttat tttgtattct ttcgtttttt atttattgtg tgcgtatttt
    13321 ttggtgatct taccacttcc ggcaaaggag acggttgccc aaatgactgg gccaaagatg
    13381 gagctgcatt tatttgcttc atggcaacat ttcagagaca acacggtatt ggtcttgtct
    13441 gaacctagta cctacctgcc ggcaatgaag cagagtgtat ttttggaacc agcttttaat
    13501 gttttattac tcataccatt tggcatttat ttaagatatt atttccggct ttcttttatg
    13561 aaaacgatga ttgctagttt ctgcctttct ctattttttg aattgacgca gctaagtgga
    13621 ctttatttta tctacccaag accataccgc ttatttgatg tgaatgattt agtgacaaat
    13681 acattgggtg gaatcattgg ttttgtcgtt acgccgttat ttacatttat gcttcctaca
    13741 agaagacgaa tggatgaact ttcttatgaa aaaggaaaga ctgtctcatt gactcgacgc
    13801 ttggtcgctt atttgatcga ctggacattt ttatctctta ttaatttggt agcggcgatt
    13861 ttctttaaac ttgcgttcga ttcagggaca gttacttctc atatctggtg gcttttatta
    13921 gaagtagggg tatactttat gattgtaccg tttataacca atggatggac acttggcaaa
    13981 aaaattgtgc gtatccgttt ggtggaagaa gaccgcgaac gaatttcttt aaaagcattg
    14041 atgatccgct acgggtatct gtactttctt ttttatggtt taagctggtt ggggcagttg
    14101 cttcagcaaa tcatgaattc gacaacgaat acggtgttgc agatgagtgc agtattcatt
    14161 tctctcggtg tcttgttact gcaaggtatt tttattttaa atatcttgct atcatttttg
    14221 agaaaaaaac ggattctgtt ttatgaaaaa gctagtcaca catatactgt tagcacgatt
    14281 ggccaaacaa aggatgatga ttctcgaaat tagctgagca aaaacccttt gtctgtgtta
    14341 taatgccaaa gtcgtgataa tttaaagaga agaggtgcac gaatgaataa ttttgatata
    14401 ttccgcttca aaccgtttat caatgaagcg ttaaccgcaa agggcttcaa acagcccaca
    14461 gaggtccagg aacgtttgat tcctttaatt gcaaaaggaa aaaatatcat tgggcaatct
    14521 caaactggta caggaaaaac acatacgttt ttgttgcctt tgtttgaaaa aatcaatgca
    14581 gaaaaagagg aagtacaagt tgttattaca gctccaagta gggaactagc tgcacagatc
    14641 tatgaagcag ctacacaaat cgctaaattt tctacaccgg agatccgtgt ctcgaatttt
    14701 gtcggcggta cagacaaaca gcgtcaatta aatcgtctgc atcaccaaca gccacaggtc
    14761 gtagtaggca cgcctggtag aatattagac atgatgaatg aacaagcgct gaagattcac
    14821 accgcatttg catttgttgt cgatgaagcg gatatgacat tggatatggg ttttttaaat
    14881 gaagtagacc aaattgcaag tcgattacca gaaaagctgc agtttttagt tttttctgcc
    14941 actattccag aaaaacttcg tccattctta cgaaaataca tggaaaatcc aatcattgaa
    15001 gaaatcaaac caaaagcagt tatttctgaa gacattgaaa attggctgat ttcaacaaaa
    15061 ggtaaagatg agaatcaaat gatttatcaa ctgctgacaa ttggtcatcc ttatttagca
    15121 atcgtatttg ccaatacaaa acagcatgtg gatgaaattg ccgattactt gaaaaaccaa
    15181 ggcttgaaag tagcaaagat tcatggagac atcaccccaa gagagagaaa acgagtgatg
    15241 cgacaagtac agaatctgga gtatcaatat gttgtagcga ctgatttagc tgctagaggg
    15301 atcgatatcg aaggtgtttc tcatgttatc aatgcagaaa ttccagaaga tctagacttc
    15361 tttatccatc gtgtcggacg tactggacgt aatggcttaa aaggaacggc cattacgtta
    15421 tattcgccaa atgatgagca agccattgat gcgatagaaa aattgggcgt ctcatttgaa
    15481 ccaaaagaaa tcaaaaacgg ggaaatcgtc aaaacatacg atcgtaatag acgtgccaaa
    15541 cgagaaaaat caaaagatgt attagatccc acgctgattg gtcttgtgaa gaagaaaaag
    15601 aaaaagatca agcctggtta taagaaaaaa atcgattggg caattgcaga aaaaaataaa
    15661 aaagaacgca aaatcgaacg cagacagcaa agccgtactg ctcgtaaaaa taagaaaaat
    15721 tcacataaat aaaaaacaag aggttgtgac aaaaggcgtg tcacaatctc ttgtttttaa
    15781 gctagacttt tttccattcc gatacaaagt acttcatctt caataaaagg ttctgtgcaa
    15841 gtatgatacc cttgtttttc ataaaaatct acagccgtca tttcagctac tacataacta
    15901 gtctgacaac cttctgaaac cgcccgtttt tcagcagctt caagcaactg accgcctaac
    15961 ccttttcctc ggtattcttt tgctacgcaa aatcgttccg gcttgaagca agtaggtgtt
    16021 ttcttttcat aacgcaaagt agctactggt cttttttgat caagaaatag ataataggtt
    16081 tgttgcggga tatcttttcc gtcgaattct agttctgcag gaatcccttg ttctaataca
    16141 aaaacttgat atctcaaaaa gcagctagct gcgcgtatcc attcatccgc tccaactatt
    16201 tttttcatca tattcctcct tgtcatgaat agcagagatg tattgattcc tttattctta
    16261 tcgtaccaaa aatcgtggag tatgaccaaa aggaaatgaa aataaaaatt gagatattga
    16321 cagtgatcaa tagaatgcgt atactaatta agtacgaaaa ggatatgatt tgttttttaa
    16381 gactcacaga aaattctttc ttggctgaaa aaagaatagt cactaatcaa attgctccct
    16441 ttttctattt tggtagaaat accgacgcag ctcagcgtta tcgagtttaa gaggtaatga
    16501 ttacacatca ttgcaatcaa ggtggtaccg cgagttttcg tccttggctg tgatggtgtt
    16561 tttttgtatt taatttatag aaagcaggta atgacatgaa acaattaagt agtgcacaag
    16621 ttcgacaaat gtttcttgat ttcttccaat ctaaaggaca tacgattgaa cctagtgcgt
    16681 ctttgatccc cgtgaacgat ccaaccttat tgtggatcaa ctctggtgtt gcaacattga
    16741 aaaaatattt tgatggttca gttgtgccag aaaatccaag aatcacaaat gcccaaaaat
    16801 ctattcgaac aaatgatatc gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg
    16861 agatgctggg taatttttcg attggtgact atttcaaaaa tgaagccatc cactgggcat
    16921 gggaattttt gactgatcca aaatggatgg cattcgatcc agaaaaactt tatgtaacag
    16981 tttatccaaa agatacagaa gcaaaacgca tctggcatga agaagttggc ctgccactag
    17041 atcacattat tgatgtagaa gacaacttct gggatatcgg tgcaggccca tgtggtccgg
    17101 atacggaaat tttctatgac cgtggagaag cttataacga tttagcagaa gacgatcctg
    17161 aaaactatcc aggaggagaa aatgaacgtt atttagaaat ctggaactta gtattttcag
    17221 aattcaacca taagcctgat aacacatttg aaccacttcc tcataaaaac attgatacgg
    17281 ggatgggatt ggaacgcatg gtctcaatcg ttcaagatgc gccaacgaac tttgagacag
    17341 acttgtttat gcctattatc cacgccgtag aaaagctaag caaacaagta aaatatggtg
    17401 aaaacccagt aacagatatt tcttacaaag taatcgcaga tcatattcgc gccctttcat
    17461 ttgctattgg cgacggtgca ttgccatcga atgaagggag agggtatgtc ttacgccgtt
    17521 tgcttcgtcg tgctgtcatg catgggaaaa aattaggtat tgaacaagca ttcttatatc
    17581 gtttagtccc tgttgttggg gaaatcatgg taagttatta tcctgaagta cttcaacaaa
    17641 aagaatttat tgaaaaagtt atccgtacag aagaagaacg tttcaatgaa acaatcaatg
    17701 aaggtttgga aatcttaaat caagtgatcc aagaagtaaa aaacgaaaac gggacgactc
    17761 tagcaggaaa agacatcttc aaactatacg acacatatgg cttcccggta gaattaacgg
    17821 aagaggtggc agaagatgaa ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg
    17881 atgcacaacg cgaacgtgct cgctctgccc gcagtactga aaaatcaatg ggggtacaat
    17941 ctgctttatt gacagatatc aaagtagaaa gtaaattcat tggatacaca gaacaagaat
    18001 cagatagtca attattggtc atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag
    18061 gtgaagctca aatgatcttt gatcaaacac ctttctatgc agaaatgggt gggcaagtag
    18121 cggatcaagg atggatcatg aatgaagtag gcgaaatcgt agcagttgtt acagatgttc
    18181 aaaaagcgcc taatggtcaa tttatgcaca ccgtagaagt aaaagcacca atgagtgaag
    18241 gacaaaccta tcatctattg attgatcaat cgatgcgcaa tcggatcatc aaaaaccata
    18301 cagcaaccca tctactgcac aaggcattga aagaagtact tggcgagcat gcaaatcaag
    18361 ctggttcttt agtcgctcct ggacatttac gattcgactt tacccacttt ggtcaaatca
    18421 cttcagatga attagctgaa atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc
    18481 cagttgaaac aattgaaaca gatatcgata cagccaaaaa catgggtgca atggctttgt
    18541 ttggtgaaaa atacggacat gacgttcgag tagtaaatat tggcggctgg tcaatcgaac
    18601 tgtgcggagg cacacatgta gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg
    18661 aatcaggaat tggtgcaggc gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg
    18721 aactacttga agaagaagaa aaacaactaa aacaagtagc aacaattgtt aaatcacctc
    18781 aattgaagga agttgtttca aaagcggaac aattgcaaca gcaagtaaga gagctacaaa
    18841 aggaaaatga acaattagct agcaagttag cgaatcaaca agctggtgat gtattcaaag
    18901 atccagaaac gatcaatgga aaaacaatca ttgcagcaca agtaaaagtg aaagatatga
    18961 atcagctgcg tcaattagct gaccaatgga aacaaaaaaa tatttcagat attcttgttt
    19021 tagcaaacga acaagaagga aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg
    19081 aagctggact aaaagctggc gacttgatta aagcaatcgc accgaaagta ggcggtggcg
    19141 gtggtggacg tccagacatg gcacaagctg gcgggaaaaa tcctgctggt attccagatg
    19201 cacttaaagc tgcgaaagaa tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa
    19261 acaaaagttc aaacattgaa cgaaacatga ttatgggggc gaagacaaac aagtcttgcc
    19321 ctcatacttt tttcaattgg ctggcgcatc taatcaaatg gtcggattcg gatggtcttt
    19381 agttgacgaa agtgacagaa atttttacga ttagtataaa taaaaagggg tgaaaaaatg
    19441 aatagtagaa ataagacctt tcgactagtc ttacgtgcga tcttgttagc aatcatcatc
    19501 gtacaagcga tggttccttg gctggggttt attccattag gattcattag tttaacaatt
    19561 atccatatca cagtaattat tgctgcagta gtattaggcc caaaagatgg catggtgatt
    19621 ggcttgtttt ggggaatcgc tacaattgtt cgtgcctatg ccatgccaac aacgccattt
    19681 gatacattag tgttcacgaa tccgattatc tcggtagttc ccagagtact tgttggctta
    19741 gtagcaggct tggttttcca ctggatttat caaaaaaata aatcaataac gatcagctca
    19801 gcctttgcag gggtactggg ttcattggtc aatacagtct tagtgttagg attcatggga
    19861 ttattttata caggtgcaac ggcagatgcc tatggtgtag attctgctct tttatttaaa
    19921 accttagcag gggttgcagc aatcaatggg ataccagaag caataggtgc gggaatcata
    19981 acaccattac tagcaaaagc gttatttgca gcaacaccgt tgaaaccaga ataatgacca
    20041 tcttttaata gtaataggac gcacgataga tagaaaaagg aagagaggag aggaaaacaa
    20101 tcgtcctctc catcttttta cctgagcaac aagcaaacta gaaggggtaa aaaacttggc
    20161 taagaaacaa gaagaaaaaa agaaacaaat accgcaacag cggacaaaaa aaggagtact
    20221 agcgggtatc ttaggattgg ttacgaatct gatcttgttt gtctctaagc taatgatcgg
    20281 gcttttttca ggaagtgtct ccattatggc tgatgcagtg aacagtctat cagatactgc
    20341 ttcatccata ttgactttga tcggatttaa aattgctgca aagccagcag atcaggaaca
    20401 tccttatgga cacgaacgct tcgaatatat cagtgggtta tttgtttcga tcatcattac
    20461 ttatgtagga tttcaatttt tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt
    20521 ctctttaaca cccatcgtct tttttgtcct aatcatttcc atctttttga aatttgcaca
    20581 aggcaaaatg tatcaggtta ttgcgcaatc cattgattct gaaacattga gagctacagc
    20641 aacagatagt tataatgatg tctttactac actggctgta ttgctttcag cagcaatcga
    20701 atgggtaact ggatggcgta tcgatggata tataggtttt gctttagcat tatatatcct
    20761 ttatagtgga ttgacgatgc ttcgaaattt tgtatacgaa ctattaggaa gtcgcccaac
    20821 gccagcagag attgaagaaa tggaagagca tttaaatgct tgcgatgata tacttggcta
    20881 tcatgattta ttggtgcata actatggacc caataaacgc tttgcatcag tccacatcga
    20941 agtagatgaa tcaatgaatt tgaatgtggc tcataagatt attgatctga tagaaaaaga
    21001 ctttaaaaag tctttggatg tggaacttgt ctgtcacttg gatccaatgc ctgttgataa
    21061 cgaaaaatac tataatatct tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg
    21121 gcttaatatt catgatttta gagtaattca tcaaggaaaa acgctgcaat ttgatgtagt
    21181 ggtccctgag aaattccagt ttgatgatac agaactagaa agaatcttac gtagtcaaat
    21241 agaagaaaaa atcggagatt accaattgga aattacgttt gatcacaatt atcttttata
    21301 ttaaatgaga aactataaaa agactcctta aagtttttaa tttaaagagt ctttttttct
    21361 tttaattgtt tatggagagc ctgacgtgca agattatctg ctcctttatt tttgttttcg
    21421 ggcaaccatt gcaggatcaa taaagaaaat tcggatagaa gttcttggat tctttgaagg
    21481 tagggaagga agagctcgtt cttcgttgcg tttttgtcga ccgtagaaac tagtgttttg
    21541 ctgtctgaaa aacagaaaat cgtttcattc tgtaagtcgt ttttctttag aaattctaaa
    21601 gtttttaaaa agacttcgaa ctctgcttga tggtttgtcc ctatagaaag cgggatagat
    21661 agctgttctt gtttaccttg atagacaatc aagattccgc ctccactagg accagggttg
    21721 ccttttgtag aggcatcaat gtatactttt aacataaaaa gagattctcc tttttgttca
    21781 aactaatgtt atggtaagat tatatcatta ctgaatcggt gagaggttag agaatgaaaa
    21841 aaaatattcg atggcagcct gaactggcac atacgatcat ctattggtca ttgaccttga
    21901 tcattctgtt ttttagtctg attctttctt tagaaaacac aggtccttat tggaaaagca
    21961 attttgtttt aggtattttt cttgtttttc tatttctagg tttgcgacgt tcttttattt
    22021 taaaatcaaa ctctgtgatt atacgttatg ctgctttttg gaaagaccga gaaatatctg
    22081 tcggacagat tcgatctttg acgccaacta atactggggt gcaaatgatg ttgcaagatg
    22141 agaaagcccc atctgttttc atgatgcgaa aaaaagatca agtagtcctt tttgatcatt
    22201 ttgaaaaaaa cggacccaaa tcagaatcaa ttccttatcc ttatacgaaa aaaagaaaaa
    22261 gctaatttta aaaatcagaa cctaaaaaaa cgaattttta caggaaaaga aaagagtgag
    22321 acgtacggtt catcccgttt agcgtctcac tctatttgtt tatctattca tgcatactag
    22381 tatcggtagt ctgaacgttg cctttcttga catgggcagc ttgtgttcct cgattccctt
    22441 cgatgatctc aaattcgacc acttgatttt cttccaaagt tttgaatccc tcttcttcaa
    22501 tcgcagtaaa atgtacaaaa atttcttctg tgtcatcata actgataaag ccgtatcctt
    22561 ttttgttgtc aaaccatttt acaattcctg tggtcaaagc actcacgacc tttctaaagt
    22621 ctgatcatag ccttcattat aaagagtatt tataagtacg tcaaataaat taaaaacctg
    22681 taattttatg ttttttttat tataaaaaaa ggtataattg gcatatctta acaaagaaga
    22741 tattttgttg aataaagttc gtgaaaaggg gtaatcaaat gaagaatgat ctgcaaattt
    22801 cacaagaaac agagctagaa ccgattgttg aagtggctag taaattagat cttaaggaag
    22861 acgatttgga actgtatggg aaatataagg cgaaaatcaa tttttctgga atcaatcgat
    22921 caaaggataa cgctgagggg catttgatct tagttacttc catcaatcca acaccagctg
    22981 gcgaaggaaa gtcaacgatc actgtagggt taggagacgc gctcaataaa atcaataaaa
    23041 aatctgttat tgcattgcga gaaccctcac ttggtcctgt gatgggaatc aaaggtggtg
    23101 ctgcaggcgg aggatatgca caggtattgc ctatggaaga tatcaacttg cattttactg
    23161 gtgacatgca tgctatcaca acagctaata atgcattatc tgctctttta gataatcata
    23221 ttcatcaagg aaatgaattg aacatcgatg cccgccgagt gatttggaaa cgggtagtcg
    23281 atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt gggtgggcca atccaaggtg
    23341 ttccaagaga agatgggttt gatatcacag ttgccagcga aatcatggct atcttgtgtc
    23401 ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag gatcgttgtc ggctatacat
    23461 ttgaccgtca gcctgtgact gctggagact tgaaagcaga aggcgctctg gcattactgc
    23521 tgaaagatgc aatcaagccg aatcttgtac aaacaattta cggaacacca gcttttgtcc
    23581 acgggggacc gtttgccaat atcgctcatg gctgcaatag tattttagct accaagacag
    23641 cattgagatt agcagattac gttgtcacag aggcaggatt tggtgcagat cttggcggag
    23701 agaaattttt agatatcaaa gtacctaact tgaaaaaagc gccggatgct gtggtgattg
    23761 tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa aaaagatgaa cttaaaaatg
    23821 agaatcttga tgctttgaaa gtaggttttg caaatttaaa gcgccatata cgtaatatgg
    23881 aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt cgtaacagat acagacaatg
    23941 aactcacact gttggagcac ttgtgtgaaa atcaggggat tattgccaag agagcgagtg
    24001 tatgggcaga tggagcagaa ggcggcgttg atttagcaaa agctgtagta cgattgattg
    24061 atagaaaaga agctaattac aagccattat accgcctaga agaaacgata caagaaaaga
    24121 cagaaacaat cgtcaagaaa atttacggag gaaatggtgt tgttttcagc aaaaaagcgc
    24181 aaaaacaaat agaagagttt acgaagaatg aatgggacaa actgccgatt tgtatggcaa
    24241 aaacacagta ttcattttct gatgatcctt cactgctagg tgcaccggaa gatttcacca
    24301 tcacgatccg cgaaatcatc ccaaaattag gggcaggatt tttagtagcc ttgactggag
    24361 atgttatgac gatgcctggt ttgcctaaaa aacctgctgc attgaatatg gacgtgacaa
    24421 atgacggaga agtgttggga ttgttttaag cttgattata atctaccgta tactaaataa
    24481 ctttcattaa acatcgaagg agctgtattt tgactacagc ttccagactg tagacaaact
    24541 cctaaattgg gagtgttgtt tgcagttttt ttgttttata ctcattgtat aaactattct
    24601 gggagtgatt ctaatgatga gaaaacaatc gattgaaggt agaaatcaat ttgctatgtt
    24661 aacaattgat gatcttgtac ctaaagatca cttagttcga aagattgatg ctgcgattca
    24721 gtttgatttc atttatccca ttgtagaatc tacgtattcg acccatggtc gtcccagtat
    24781 tgatccagtg gtcttaatta aattagtatt tatccaatat ttatttggta tccgttcgat
    24841 gagacagact attaaagacg ttgaaacaaa tgttgcttat cgttggtttt taggttattc
    24901 attcgaggat cctatcccgc atttctctac ttttggtaaa aactatgttc gaagattcag
    24961 agaaa
//
LOCUS       DACDNO010000042        24867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDNO010000042 DACDNO010000000
VERSION     DACDNO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.042"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IWI22_001773"
     CDS             complement(<1..626)
                     /locus_tag="IWI22_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9169164.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="IWI22_001774"
     CDS             899..3370
                     /locus_tag="IWI22_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9169165.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="IWI22_001775"
     CDS             complement(3473..5209)
                     /locus_tag="IWI22_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9169166.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="IWI22_001776"
     CDS             complement(5206..5910)
                     /locus_tag="IWI22_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9169167.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="IWI22_001777"
     CDS             6041..6544
                     /locus_tag="IWI22_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9169168.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="IWI22_001778"
     CDS             6504..6842
                     /locus_tag="IWI22_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9169169.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="IWI22_001779"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="IWI22_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9169170.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="IWI22_001780"
     CDS             8393..8971
                     /locus_tag="IWI22_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9169171.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="IWI22_001781"
     CDS             8975..9496
                     /locus_tag="IWI22_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9169172.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="IWI22_001782"
     CDS             complement(9575..10129)
                     /locus_tag="IWI22_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9169173.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="IWI22_001783"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="IWI22_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9169174.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="IWI22_001784"
     CDS             10669..10920
                     /locus_tag="IWI22_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9169175.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="IWI22_001785"
     CDS             11045..11836
                     /locus_tag="IWI22_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169176.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="IWI22_001786"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="IWI22_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9169177.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="IWI22_001787"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="IWI22_001787"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9169178.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="IWI22_001788"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="IWI22_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9169179.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14019..14312
                     /locus_tag="IWI22_001789"
     CDS             14019..14312
                     /locus_tag="IWI22_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9169180.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            15155..17482
                     /locus_tag="IWI22_001790"
     CDS             15155..17482
                     /locus_tag="IWI22_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9169181.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            17608..17937
                     /locus_tag="IWI22_001791"
     CDS             17608..17937
                     /locus_tag="IWI22_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9169182.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            17977..18981
                     /locus_tag="IWI22_001792"
     CDS             17977..18981
                     /locus_tag="IWI22_001792"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9169183.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            18978..20407
                     /locus_tag="IWI22_001793"
                     /pseudo
     CDS             18978..20407
                     /locus_tag="IWI22_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            20613..21785
                     /locus_tag="IWI22_001794"
     CDS             20613..21785
                     /locus_tag="IWI22_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9169184.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(21947..22402)
                     /locus_tag="IWI22_001795"
     CDS             complement(21947..22402)
                     /locus_tag="IWI22_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169185.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            22992..23414
                     /locus_tag="IWI22_001796"
     CDS             22992..23414
                     /locus_tag="IWI22_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9169186.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(23580..24773)
                     /locus_tag="IWI22_001797"
     CDS             complement(23580..24773)
                     /locus_tag="IWI22_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9169187.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
BASE COUNT         8402 a         4581 c         4925 g         6959 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatc agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaacgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt
    13861 ggtaggggag aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc
    13921 actatgataa tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac
    13981 ataaatggag tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc
    14041 aagaaaagat gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa
    14101 gttcaacaaa aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag
    14161 tgacttatac agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt
    14221 atgtcgaaca aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc
    14281 gggcatatca gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa
    14341 gttcaagtat gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc
    14401 tataatacaa agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag
    14461 cgattcgcag ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa
    14521 tcaattggat gcgttacatg aataaagata attctctttt atttgtacga ataaatacca
    14581 aaacgtacct cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc
    14641 gttcttgaaa caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc
    14701 tgagcgttag aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga
    14761 atacaagatc agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg
    14821 acacatttct cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat
    14881 aatcccgtcc taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa
    14941 ttaatcaagt tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa
    15001 aataaaatct ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa
    15061 agccccaaac tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa
    15121 aggattattt gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa
    15181 taaaatccaa cgtatatgtc aaaaacattt tgtttctaga ctcaaagctg tatctaaaag
    15241 aaaatactta catcccatta tgatttcgat catcttgatt tttactttct tagggagctt
    15301 atcaagtatt tatgtaaacg tttatacaga aacgtcaaat tttgctaacc aaacgacaga
    15361 ggagacaaat agtccaccct attctgaaat acagtccata gaaagcacca atcagtctag
    15421 caacaacgag acaacctcaa ccacatcctc gactcaactg attgatccga cagaaaaaga
    15481 aatcacagca gcaaaagcag caggggagaa agcttattca gaaacaggta gaagccagac
    15541 atttagtact gttgcggcca ctgcgaattt caacggacat accataccag atggatatac
    15601 agtgatatat gccaaagatg gagtcattgt tgcggttaac ggatcgatga gtgcggataa
    15661 aactgcgtta caaggcgtaa ccaaagcaat cgcttttatt gataaccgtc cagatatcca
    15721 taatctgttt attggctcca cagtaaatgg tactactatc acattcccaa aaagtatcta
    15781 tatgggggtg gctaccgctg gaaatacgat cgattcagtt acacccaatg cggataaaag
    15841 ttattctttt acaccctcaa catggggagg gcttcctgcc ggaggaggta tgagtacttt
    15901 ttcaatcggt atacagttag caaatgatat tccattcacg gggtactcga tcaaaaatgg
    15961 gcagatcgtt gatggtaagg gaaatgtttc aaattttcca acctatcaag cagcaacggt
    16021 tcaggtcaat gcgaatcgga caggaacttt ttcttataca gtcaatggtg gagcagcaaa
    16081 aatcggaacg tacgggacta cattatctgg aatccatgtg ggtgacaaag tggttatcac
    16141 tccaacagca gtaacgggct attcttttgc acaaacaaac ggttctttca cagccctaga
    16201 aagtaacgtc gatacggtga cctatacagg caaacaaaca ccaatcacta ttaagtatca
    16261 agactatttt ggacagatga ttgctccttc taaaataatg aatcttactt acggaagtgc
    16321 tgcacaagac ctgacgacta atacgcctac tatcaacgga tataccttta cagctgtttc
    16381 agcgacagaa acaaagaacc aatcagcaat gagtgtttta gctagtttag atacaaatgg
    16441 aaatgtaatc gtaaaggacg caaatggaaa acagatttct gaagtcatcc tatattataa
    16501 gacaaatgca acagtatcaa tcaatgcaaa tggcagtaag tactatgatg gtctttctgt
    16561 tgtaccgatc gttaaatatg cctttaatga taaaacagaa tcaacatccc ttttaaacaa
    16621 tgatctcagt attgatcaaa tcacatggaa tccaactgat ttcaaagcga tgaatgaaaa
    16681 gggagttgaa gtaactgcgc ctactgaaat tggaacctat acgtcttggc aattgacaaa
    16741 aagcggacta gctaaactag ctgccagaac aaactattta tttactatcg tgcagacatc
    16801 tgatgtctat acgattaagc aaatcagcgg tgcggtagag ctcagtggca gtaagacata
    16861 tgatggaaaa gcaggtgtcc cgtcaatcca tgtcaagcta gcagagggtg tcactagttc
    16921 gcttactcca gtacaagtgg cacttagcag tatggattac acagcagatg cacaatcagc
    16981 aaaaaatatg gttaatgctg gcagctatac aattaaacta acgaatagcg gaatagacaa
    17041 agtcaaagca gcaaatccca agttgtcatt tactgatata gccaacacat caggaactta
    17101 taccatctat aaagctaacg cagtgattac tgtcgatgac gctgccttta attatgatgc
    17161 ccaaagtcac tcgatttcgg ttggaaatct ccacgttacc ggtgttgtct taggcgaatc
    17221 attggattat accctaacag ttaacagccg gacagatatt ggatcacaaa tagtaacgat
    17281 tagtttaggc gaaggaagtg taaacaataa ttatacgatt atcgcaacag ataccgctaa
    17341 gttgacgatc aatccgactc tcttgcttcc ttcgacaggt gggataggtc tactgccctt
    17401 tgtcttttta ggactgatct ttattttttc tggcttcttt tattttatcc atcgtaagaa
    17461 aaaagcaggt gaacagcgat gaaaaaaaca aaaaatgtac gtgtggatat atttgctgtt
    17521 ctctttttta tcctactggt cttttttata ggttctccga ctgtgtcagc tagagaatca
    17581 cctattacat ttactttgca taatcgaatg ttcgaaagcg ataagatgcc aagtatcagt
    17641 caaaacgacg gaataaatat cccggataac tttcaaggag taaaagggtt gaacggcgtt
    17701 actttcgcta tatatgatgt ctcggatgaa ttttacaagc tgcggtcaga aggaagtagt
    17761 gtggaagacg cacaaagaaa actagcacaa aaaagtgata gtgaaaaaat actggcagaa
    17821 aatgtgacaa aaacagtaga cgaggaagag ggcattgctt cttttagtgc atcggacaaa
    17881 gatgagcaag gtcgtgacgc tgtttaccga tttgcggaaa taaagacttc agattaagta
    17941 aaagaaaaat cagcaccatt tgtcgttgtg cttcctgtga cagattcatc taatcataaa
    18001 ttgacgacca tccacttgta tccaaagagc gaacaaaaaa tacctgctgc actcacatta
    18061 acaaaaacag tagagaataa gcagacagat tttgcagatg gggataaagt tccttatctc
    18121 atcaccgtaa caattcctga gaacatcaac gagataggaa cctatacgat caaagataca
    18181 gctgatcctc agttatggct agaaccagaa acgatcgatt ttttgattgg tggagacaag
    18241 attcatacat ttcacacaca aaaaacaaaa catggttttg tcttatcaga ttctggaaaa
    18301 gatcttagtt cgtttgctgg gaaaaaatta acgatccgtt accaaatgac attgaaagaa
    18361 aacagtgaaa aaacgacatt tgataacgat gtacgtttga cgactgacaa tcaaacagta
    18421 gaaacacatc ttgccatcca aacaggaggc aaacaatttg tcaaagtgga tcggcaaacg
    18481 aaacaaaaac tagcagatgc tcaatttcta gtcaaaaaaa atgaccaata cttagcgcaa
    18541 gaaaacggga taaatcattg ggtagccaaa gatgattcaa gagcaacgat ttttacttcc
    18601 agtaaagatg gaacattttc tgtccatgga ttgtcttttg gaagttacga actagtggag
    18661 attaaaccgc ctaaaggata tatattgagt caatcaacca ttctctttga agttgaaaaa
    18721 ggcagttatg atccgcttca tcctatacca ttggaaatca tcaacgaacc aaaagcaaat
    18781 ccgacgaata atggaaaaac gccagttcag atgcagacaa atggtccatc aaatgaaaaa
    18841 aatggaggaa gctttccaaa aacaagcgaa gagatgcttg gaggtttctc aattttaggg
    18901 ttgttgctag tcctttcaac agggacagct tggttttata aaaaacaaca aaaaggaaac
    18961 aataggaggg aaataaaatg aaaaacatca aaacaaaaat atggacgttt ttaggaactg
    19021 ctatcatgct gcttcctttc gttctcggcc taggaactgc agaggtttct aggccgtatc
    19081 acctacacca gaaaatgtta cggttaattt gcataaattg aaattcactt cagcaccaga
    19141 aaatcaaata aacaacggaa cggaattgac atttccgaat agcgaaccgt tgaacggcgt
    19201 tgaattcaac gtatatgaca tcactgctac gtactatcca agtaaagaca cagcagttcc
    19261 agcagacgct acacccttcg caagtgtaac gacttctggt gaaggattag ctaacctgac
    19321 tcttccaggt aaatctgatg ggaaagatgc tgtatacgtc tttgtagaaa caccaaagcc
    19381 cggtgtcgaa acgtcgccaa atatcgtgtt aagcttaccc ttttataacc cgaattcatc
    19441 agaccctgac aaacctttag acacgattca cctatatgct aaaaatgtca tcaaagaaag
    19501 caatatcaaa atccagaaag ctgggaactt ttcaggggta gataagttag caggggcgca
    19561 attccagatc aaaaattcag agaatcaatt cgtgaccggt tttgatgaaa acactggtgt
    19621 cgctctctat gatcctgctg agtcagcgtt gacattcaca actgatactg aaggatccat
    19681 cagtgtcaat ggattaatag acggcaatta tactttagtt gaaacaaaag caccagatgg
    19741 ttatcaatta accaatccac aaaaagaaac tacttttata gtaaaaaacg gaaaattgca
    19801 acaaacaacc ggtatcatcc atggcgcaga tgaagccaat cttgggaatc caaacggtgt
    19861 agtcaatcgg gaaaatacaa ctacggataa cacgattact gtagaaaatg tccaaaacta
    19921 cggtgatttc cacttcatca aacaagatgt gaacacgcaa gaaaaattag ccgatgcaga
    19981 attttctatt ctaagagcaa aagatccaac tgttaaatta atgaaaaaaa tagcaaatct
    20041 tgcctcagga gaatacccat atgcttggtc agatgaagtc acaggttctg actgggaaga
    20101 agtgaaattg atttctgatt cagaagggaa attttcaatc gaagggctga aacaaggggc
    20161 atactgcctt caagaaacaa aagcaccaga aggctacgca ttacctaaga atgaagctgc
    20221 gtacactgaa tttacagtag atgatgatcc aacaacttct gatgaaacaa cgatcgataa
    20281 ccaacctaaa ggaatcttgc cttcaactgg tggtatgggg atcatcgtat tcatcttggt
    20341 aggtactgct ttagttggag gagcagtgat ctacttcaaa aaacgccatc aagaaacaga
    20401 agcttaaact attggaaaca aaaatcatac gataaataaa cgatagtgat tcaaatgggg
    20461 agaaaggttg tgacaagtgg tcggtctctt ggtattcgct caacaaacgg caaaatagga
    20521 ggaactattt tgccgtttgt tgagcgaata ctcaggtcaa ggcgctgatt tcacaacctc
    20581 ttcatataca tattagaaaa taggtggaca tgatgaataa aagcagaata agaatactgg
    20641 acatcttcat ggcaatcatt ttagtcgttg ggatcgggat cttttcttat ccttttgtag
    20701 aagattcact gaatgatttt cttgcccagc aaatgatcat ccattatcaa aagcaagcat
    20761 cgaataagaa ttcagaggaa ataaaaaaac agcaggaaaa aatgacaaag aaaaaccaac
    20821 agttggcaga gcaaaacgta tcaccaggga taggcagttt caaccaagca gttgatgcaa
    20881 aagcattaaa agatttacct agtaatgcct tttttatgga acatatgctt ggagtcatcg
    20941 aaatcccaaa aatcaatgtc agcttaccta tattcgatca gacaacggaa atatttttgc
    21001 aaaaaggaac gtcattattg gaagaaagtt cttatccgac tggcgggaaa aacacccatg
    21061 ccgttctttc tgggcatcgt ggactcccag aagcaaaatt atttacagat ttgcctaagt
    21121 tgaaaaaagg agaccagttt ttcatccaga tcaatggaaa gacattggcg tatcaagtag
    21181 aaaaaatcca agtggtctta cctgatgaag tagattcttt gggtatccaa aaaggcagag
    21241 atctcgtgac tttgcttacc tgtactcctt atatggtcaa tacacatcga ctgcttgtga
    21301 cggggcatcg tattccttat caagcaaaag aggcaaaaaa agcgatccaa ggaattgatc
    21361 aatggaaaaa atggaaattt tttatttggt ttatcggtat actacttgga tcaatcggct
    21421 tagtttggct actcatcgct tatttagatt ctttggccat cgctaagcga aactatcctt
    21481 tatcttttta tgtaaagaac acaaacggac gaccaatcga aggaatggtc ttttctgtaa
    21541 aaacgctcaa cggcaaacac tatattacta gagaaaaagt tccatttgtg aaagcttctg
    21601 atgaatacgg actagtgaga ttttctgatt taaaaggcag gaactatcgc ttgcaacatg
    21661 aggagctcct gcttaaaatc catgtaaaac ataaacattc gaaacagttt tcaatgaaat
    21721 tgaaaaaagg aagatataaa ctgcgaaaag aaaaagaggt gtactatttg attgagaaag
    21781 aatgaatgaa gatgtccaaa aaagcagagg gaacagacta aatgaaaaaa gagagaagct
    21841 gacgaaatgg acgtgtaaac tgctattttt ggtttactat ctggaacgcc agcttctatt
    21901 tttttatgtt tcagaagaat tttattatta tagtataact tttgaattag tttccataac
    21961 atttggagca aggggttaaa ccacgagctt gagcttcgct taaagtggca ggggtatacg
    22021 taccatttcc acatttatgt gtatggtatt ttgaaccggt cggtgtgaca tagacagttt
    22081 gttcattatt ttgctcagga gctgcggctt gtgcctgctg agaggcctgt tcagctgctg
    22141 cttgctgtcg cgcctgttct gcttgttgct gtgcgattgc ttcttgttgc gcttgttctg
    22201 cttgttgtcg tgcaatttct tcttgctttt tccgttcttc ttctgcttct tttgctttga
    22261 ttgtggaatc aattgcagca agacggcttg tcaactcttg tttgttttca ggtagcgctt
    22321 ggatagctgc tgaagcagtt gcatagtttt cttttgtagg atttgcttca gcttgtgaca
    22381 aagcagattc cgcttctgtc actttttttt ctagtgcagc tttttccgct ttttgtttct
    22441 cttcttcttt tttcttttca atttgttctt gttctttcga tgaatctgcc gtactttcgg
    22501 agatcgtggt actgctgctg gaagcctgag ctgaacttgt gctttgagta gtagacgtag
    22561 tagcaccaca cccactcaac aaacttcctg cgaccaaaaa caatgcaact aattttttca
    22621 ctaaatttcc tccttaagta aaaaacaacc gttttcaata aatgtccttt tcaattgttt
    22681 acttacttgt tataataatt gtgttatgaa tagtgattga ggaaacttaa tctaatcccg
    22741 ttttgataca cgggattttt tgctatagtt ctgttattaa gtatgcaata ataaaatatg
    22801 tacgttattt tattattaga ttattgacat ttatataata aacgattaaa tgattcatat
    22861 caatagaata cttaggaaaa aaagagaaat aataacaggt ttatgcacta ttgacggtgt
    22921 gacaatatga tatttgtatc cgtttttttt cttgtatgta gtatagtaaa gtagatacag
    22981 gaggagaaaa tatgcagact tttttaacaa aagtttctca aaagaaaatt aaaatgattc
    23041 atttactgct tgaaacagaa cgttggtgtt caatagaaga actacaaaac gaattgaagg
    23101 tatcaagtaa gtctatcttg aattatctaa cagatttaga agaattgttc caaaaatatc
    23161 ctgataaggt cattttgaag aatgagaata atcggcgctt ttctatggaa aagcaggaga
    23221 acttcccaat ctacattatt tatcttcatt tctacagaaa atcatataat tatcatttga
    23281 ttgaattcat gtatcaacat cctgagaaaa atttggaaga ttatgcagac gctcaatata
    23341 cgagtgtatc cacagttttt cgttatgcta agttattagt aaagtacttt gaaatgacgg
    23401 acgtcaaaga atagtagccg ccagatagaa ggaaattagc agttgaaaaa gctgccctgc
    23461 atcaagtaat acgaccagcc aaacggaaac agtgtctcat gtttttgatt tggctggtct
    23521 tattaccgca gatgcaagct tttgccacac ctctttcttg tcatttacag actcaactat
    23581 caatgctgca agctttccct taatgctgta acaccagtaa gagttgtcaa gaccagcaga
    23641 ctgactaggt aaataccgct caaaaacaag attacggcag ttaaactata atgatccatc
    23701 aagaaaccga tgacgataga agaaaaacca ccaacagctc gtccaaggtt cataatgaaa
    23761 ttattcgccg tcgcgcggat ttctgttgga tacaaactac taatcactgc gccatatcca
    23821 ccatacatgc cattgataaa gtagccgact atcacagcgg ctgctaccaa actccactgg
    23881 ctatgggcta acacaagtag atagacagat aaggatgaac aaattaaaaa caaagaaaag
    23941 gctagtttag gccccaattt atccatgatt atgccaaaag tcatcatccc caaagacatg
    24001 cctaaaatag tactgaccat ccacaatgaa gacccagaaa ccgaaagccc taatcgattt
    24061 tgcataattg aaggcagcca attcattaac ccaaaatagc cggcaatttg gacagttacc
    24121 atgatactaa ggccacatgt ttgccaagca agatgttttg attcaaacag tctgctaagt
    24181 ttggttttct tttcggcaga ttgtacagat ttgaattcct ttggttcagt caagccgcga
    24241 cgaatgaaca agaccatcaa caccggcagt acacctacga catacaacat cttccaacca
    24301 aacgttggaa tgatcacagc agctaaaatc gcagccaaag ccgcaccgac ctgcgcacca
    24361 ataccagcaa ccgaagaagc tcgtccaatt tgtttttttg aaaaactttc ggaaatcaag
    24421 gacatacacg ccccgtattc tcctcccgct cctacacctg cgataaagcg aaagagataa
    24481 accaagtaga tgtttgaagc aaaatacatc aataaagaag caatcgaaaa cagaaggacc
    24541 gtttgagaaa atactttcac acgtccatat ttatcagcta tgatgccaaa aaaaatacct
    24601 ccaatgagca tccccaaatt agtgatcgtt gaaatgaaac ccgcttgcgt accactaata
    24661 tgaaaagaag taatcattga agataatgaa aaagctaaaa acataatatc catattctct
    24721 aaagcaattc ccgccgatgt cgataataat aactttcgct gatacgttgt catttttata
    24781 ccccttttcc taaaatttcc ctaaattgtt aaaagtatat ccaaaaaaga ataaaaaaac
    24841 aagacttttt tatttgaaat aatacac
//
LOCUS       DACDNO010000043        24271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDNO010000043 DACDNO010000000
VERSION     DACDNO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.043"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..572
                     /locus_tag="IWI22_001798"
     CDS             <1..572
                     /locus_tag="IWI22_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9169188.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="IWI22_001799"
     CDS             576..1217
                     /locus_tag="IWI22_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9169189.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="IWI22_001800"
     CDS             1214..2131
                     /locus_tag="IWI22_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9169190.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="IWI22_001801"
     CDS             2128..2799
                     /locus_tag="IWI22_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9169191.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="IWI22_001802"
     CDS             complement(3340..3828)
                     /locus_tag="IWI22_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9169192.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="IWI22_001803"
     CDS             complement(4029..4295)
                     /locus_tag="IWI22_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9169193.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="IWI22_001804"
     CDS             complement(4295..4558)
                     /locus_tag="IWI22_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9169194.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4551..4823)
                     /locus_tag="IWI22_001805"
     CDS             complement(4551..4823)
                     /locus_tag="IWI22_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169195.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5034..5219
                     /locus_tag="IWI22_001806"
     CDS             5034..5219
                     /locus_tag="IWI22_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9169196.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="IWI22_001807"
     CDS             complement(5361..7184)
                     /locus_tag="IWI22_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9169197.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="IWI22_001808"
     CDS             complement(7782..10487)
                     /locus_tag="IWI22_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9169198.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="IWI22_001809"
     CDS             complement(10793..11740)
                     /locus_tag="IWI22_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9169199.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="IWI22_001810"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="IWI22_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9169200.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="IWI22_001811"
     CDS             complement(12375..12737)
                     /locus_tag="IWI22_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9169201.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="IWI22_001812"
     CDS             complement(12748..13869)
                     /locus_tag="IWI22_001812"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9169202.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="IWI22_001813"
     CDS             complement(13885..14235)
                     /locus_tag="IWI22_001813"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9169203.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="IWI22_001814"
     CDS             complement(14365..15876)
                     /locus_tag="IWI22_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9169204.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="IWI22_001815"
     CDS             complement(16198..17550)
                     /locus_tag="IWI22_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9169205.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="IWI22_001816"
     CDS             complement(17703..18779)
                     /locus_tag="IWI22_001816"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9169206.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="IWI22_001817"
     CDS             18975..20720
                     /locus_tag="IWI22_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9169207.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="IWI22_001818"
     CDS             complement(21000..21437)
                     /locus_tag="IWI22_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9169208.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="IWI22_001819"
     CDS             complement(21421..22794)
                     /locus_tag="IWI22_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169209.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="IWI22_001820"
     CDS             complement(23116..23604)
                     /locus_tag="IWI22_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9169210.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="IWI22_001821"
     CDS             complement(23626..24228)
                     /locus_tag="IWI22_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9169211.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4488 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DACDNO010000044        23820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDNO010000044 DACDNO010000000
VERSION     DACDNO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.044"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(45..779)
                     /locus_tag="IWI22_001822"
     CDS             complement(45..779)
                     /locus_tag="IWI22_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9169212.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1106..1516
                     /locus_tag="IWI22_001823"
     CDS             1106..1516
                     /locus_tag="IWI22_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9169213.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1509..2210
                     /locus_tag="IWI22_001824"
     CDS             1509..2210
                     /locus_tag="IWI22_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9169214.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2210..3796
                     /locus_tag="IWI22_001825"
     CDS             2210..3796
                     /locus_tag="IWI22_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9169215.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3815..4036
                     /locus_tag="IWI22_001826"
     CDS             3815..4036
                     /locus_tag="IWI22_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9169216.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4033..4794
                     /locus_tag="IWI22_001827"
     CDS             4033..4794
                     /locus_tag="IWI22_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9169217.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5174..5683
                     /locus_tag="IWI22_001828"
     CDS             5174..5683
                     /locus_tag="IWI22_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9169218.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5758..6297)
                     /locus_tag="IWI22_001829"
     CDS             complement(5758..6297)
                     /locus_tag="IWI22_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9169219.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IWI22_001830"
     CDS             complement(6437..7048)
                     /gene="rpsD"
                     /locus_tag="IWI22_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9169220.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7364..8161
                     /locus_tag="IWI22_001831"
     CDS             7364..8161
                     /locus_tag="IWI22_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9169221.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8189..8824
                     /locus_tag="IWI22_001832"
     CDS             8189..8824
                     /locus_tag="IWI22_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9169222.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8844..9617
                     /locus_tag="IWI22_001833"
     CDS             8844..9617
                     /locus_tag="IWI22_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9169223.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10025..10822
                     /locus_tag="IWI22_001834"
     CDS             10025..10822
                     /locus_tag="IWI22_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9169224.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10800..11213
                     /locus_tag="IWI22_001835"
     CDS             10800..11213
                     /locus_tag="IWI22_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169225.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11197..13842
                     /gene="mutS"
                     /locus_tag="IWI22_001836"
     CDS             11197..13842
                     /gene="mutS"
                     /locus_tag="IWI22_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9169226.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13860..15968
                     /gene="mutL"
                     /locus_tag="IWI22_001837"
     CDS             13860..15968
                     /gene="mutL"
                     /locus_tag="IWI22_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9169227.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15990..16550
                     /locus_tag="IWI22_001838"
     CDS             15990..16550
                     /locus_tag="IWI22_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9169228.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IWI22_001839"
     CDS             complement(16635..18623)
                     /gene="treP"
                     /locus_tag="IWI22_001839"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9169229.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18846..19559
                     /gene="treR"
                     /locus_tag="IWI22_001840"
     CDS             18846..19559
                     /gene="treR"
                     /locus_tag="IWI22_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9169230.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19636..20082
                     /gene="msrB"
                     /locus_tag="IWI22_001841"
     CDS             19636..20082
                     /gene="msrB"
                     /locus_tag="IWI22_001841"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9169231.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20252..20854
                     /gene="ruvA"
                     /locus_tag="IWI22_001842"
     CDS             20252..20854
                     /gene="ruvA"
                     /locus_tag="IWI22_001842"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9169232.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20867..21868
                     /gene="ruvB"
                     /locus_tag="IWI22_001843"
     CDS             20867..21868
                     /gene="ruvB"
                     /locus_tag="IWI22_001843"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9169233.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21897..22475
                     /locus_tag="IWI22_001844"
     CDS             21897..22475
                     /locus_tag="IWI22_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9169234.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22527..23009
                     /locus_tag="IWI22_001845"
     CDS             22527..23009
                     /locus_tag="IWI22_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9169235.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23126..23500
                     /locus_tag="IWI22_001846"
     CDS             23126..23500
                     /locus_tag="IWI22_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169236.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7774 a         4284 c         5115 g         6647 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaagacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg aaaagcatcg
      841 gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat aaaaaaatga
      901 attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt taaaagttct
      961 ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt tttctcaaaa
     1021 attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta gtaaaatgaa
     1081 agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca ttattatttt
     1141 cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc ctggaagtat
     1201 cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc ctgaaaaaat
     1261 cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc ctgctggtgt
     1321 aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac tgggagcagt
     1381 cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag gtgtagaagc
     1441 tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg aaatggagga
     1501 gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg actttaggag
     1561 tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg aatcctctcg
     1621 tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc tataaagact
     1681 ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg gcacttgcca
     1741 tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta atggctgcga
     1801 ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac ttcttctctc
     1861 ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct atttctgtcg
     1921 atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt gtctctacag
     1981 gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc gaaagtccag
     2041 ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca aaagcaatcg
     2101 agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac ggaatattga
     2161 ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga tgcgaaaacc
     2221 actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga aagacgtgta
     2281 tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga ttctcctcct
     2341 agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag ttgttcagct
     2401 ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc agcagtttag
     2461 cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact tttatgatac
     2521 gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta cgctgaaatt
     2581 ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat ttgcgcaaaa
     2641 gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac agcaactgca
     2701 aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag aagaaatcat
     2761 tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct ggccatttga
     2821 aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt tttttaatgt
     2881 tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct tttttttaag
     2941 aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga tttctgacaa
     3001 tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt attttcaagt
     3061 agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat attatagtga
     3121 tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac cgccctttac
     3181 agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg ggctgccagc
     3241 agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt tcgcctatct
     3301 ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta atcatttagt
     3361 tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta gacacaaaca
     3421 tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc tgactgtgtt
     3481 taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt taatgacaga
     3541 tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt tccgtaatga
     3601 gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc ttctgatttc
     3661 gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg tgactgaaga
     3721 actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc aaaaacaaaa
     3781 actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa ttttaccaga
     3841 attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac tttatgttgt
     3901 tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat tgatcaaaac
     3961 aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag cagatgattg
     4021 ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt atttatcaca
     4081 ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca agatgctttt
     4141 gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg atttcctgaa
     4201 acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca agcggtatcg
     4261 aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg gatattggat
     4321 gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta tgagacgaat
     4381 gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg ccaaaaaagc
     4441 ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg tgggatcgct
     4501 tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct ggattacgca
     4561 ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc aatgaatgcg
     4621 gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac accagtcaag
     4681 cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc tcctcaagca
     4741 agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa ataaccttgt
     4801 gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg atgataaaaa
     4861 aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa ttctttttaa
     4921 taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa acgatattca
     4981 aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag ctatttttat
     5041 ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc gacacttcag
     5101 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
     5161 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
     5221 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
     5281 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
     5341 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
     5401 tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
     5461 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga cagcatgcgt
     5521 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
     5581 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
     5641 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
     5701 aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct tttttgatta
     5761 atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat aagctgctcc
     5821 agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa atgcagttgt
     5881 aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg cagcaagaga
     5941 gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt cgatcatcca
     6001 accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg gcccaaataa
     6061 aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc caagtaaaag
     6121 ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg ttaatccaat
     6181 agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct gagaaaggac
     6241 agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc ggttcataac
     6301 gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa acaaagtgat
     6361 tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc ggtctttttc
     6421 aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga gcttcgtcga
     6481 tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc aatttttcag
     6541 cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct tttactgtta
     6601 cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg tggtatgaag
     6661 ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat tgacgtgctt
     6721 gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt tgttctagta
     6781 agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt acgaacaagt
     6841 tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt tcagttaatt
     6901 gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct ggtgcgtaag
     6961 ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga cgagaaattt
     7021 tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat aagtttttgg
     7081 agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat cttcaccttt
     7141 gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat gagttattat
     7201 ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca cgaatgtcaa
     7261 gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa tgagagggta
     7321 tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc cggtatcacc
     7381 attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg aaaacagctt
     7441 ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag gaacggctgt
     7501 ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa agattttata
     7561 cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg aattaggaac
     7621 atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt taagtaaggc
     7681 agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat tatgtggatt
     7741 tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta tgttagaatc
     7801 aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga tgtttgcgaa
     7861 tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg tcggaaaagt
     7921 gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac acgtgatagc
     7981 gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg atggaatgac
     8041 agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata ttggtggcgg
     8101 tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct atgtggacta
     8161 aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa aaaatgagga
     8221 aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg gagtacctaa
     8281 gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt tatctatttc
     8341 tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt atcagtatgt
     8401 agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact tagtcgtatt
     8461 gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga gaaaagtata
     8521 cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat tgtttgaaaa
     8581 aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt ataaacagca
     8641 aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa aggaattctt
     8701 tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg ccgattgggt
     8761 aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa gtaagtattt
     8821 ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat attgatggaa
     8881 cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg gaaaaattac
     8941 gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat gcgcaagaga
     9001 tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc ggttttttag
     9061 accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg ttcgttcaag
     9121 aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc aagcgttctt
     9181 cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct gtcttgcctg
     9241 aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca tttttcgatc
     9301 aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc tggcatgaga
     9361 acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg cacttagcag
     9421 aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa aacgaccgtg
     9481 aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca gaagtacaag
     9541 cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag gcattgaaag
     9601 agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt aataagccca
     9661 gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc gcagatgcaa
     9721 gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc aagaaataag
     9781 aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat ttctgaagaa
     9841 gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg tcatagtccc
     9901 atttttttat tttatttttc aaaactaaga aataccagga aagagttcct tttgtttgac
     9961 cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag aagaggaatt
    10021 tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag agatgattac
    10081 tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg taaatggtga
    10141 gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt tgcaagacgg
    10201 cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct ttgagtttgt
    10261 aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc ctggacaagg
    10321 gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc aggctcgttc
    10381 ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag aagaagccag
    10441 aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg aaaaacaagc
    10501 aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata cacacgttca
    10561 gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg ttgggatgac
    10621 aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa agtttctgac
    10681 tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa gcggctgtat
    10741 cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc agatcaacga
    10801 tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag ccggaaattc
    10861 aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc attcgctaca
    10921 aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga gaaaaaatca
    10981 aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca gcagaaaaag
    11041 aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg cttgttgtag
    11101 cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca actatgatac
    11161 aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac taaaaatact
    11221 cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt tttgttctat
    11281 cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc acagcttttg
    11341 gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat gtgcggtgta
    11401 cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta taaagtagcg
    11461 atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg cgaagtgatc
    11521 cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa agacaacaat
    11581 tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc cgatttaagt
    11641 acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa tgaagcctct
    11701 gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct tcgtgaacag
    11761 ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga aaacgcggaa
    11821 tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac cggcaaacta
    11881 ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa agcagtagaa
    11941 taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct agaattaagc
    12001 caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga tgaaacaaaa
    12061 acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat ccaggcaaaa
    12121 caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt tgaacgtatc
    12181 gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg tcgagtagct
    12241 ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga acaagtgcca
    12301 atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct gctttcagaa
    12361 atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga tgatccgccg
    12421 ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct cgatatgtac
    12481 cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa agagcgtcaa
    12541 gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta ttatatagaa
    12601 attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa acaaacttta
    12661 gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca aatcctcgaa
    12721 gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag agaagaagtc
    12781 aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt agatgtgcta
    12841 caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact tgtcagtgac
    12901 aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt attaggacat
    12961 caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt gctgattaca
    13021 ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac tgttctgatg
    13081 gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt tgatcggatt
    13141 tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt tatggtagag
    13201 atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat tttgtttgac
    13261 gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc aatcatcgaa
    13321 tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca tgaactgacg
    13381 gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt tgaaaaagac
    13441 ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag ctacggcata
    13501 catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc agatatcctg
    13561 catacgctag aaaatggcga taacggacac catactgttc cagctgaagt agaagagaaa
    13621 actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa tcaagaccat
    13681 atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga attatccgaa
    13741 aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat gacgccaatg
    13801 gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg cggatatcga
    13861 tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg ggagaagtag
    13921 tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac gccggcagta
    13981 ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg attgataacg
    14041 gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg acaagtaaga
    14101 tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg gaagctttgc
    14161 caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa gaaaaacaag
    14221 gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca ccactgcgtc
    14281 aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc ttgaaatacg
    14341 tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga ctggcattga
    14401 gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg gcaactgctg
    14461 gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc gcaaaaaaaa
    14521 tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt tcattgcctg
    14581 aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt tttatcaaga
    14641 acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg gtcggtcgtt
    14701 tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta aatgttcatc
    14761 cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta attagtaacg
    14821 ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat aatctacggt
    14881 ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat ttgtcctacg
    14941 cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga gagacaggta
    15001 cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat aagtctgtgg
    15061 aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca gaaaaaacag
    15121 cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa acagtggaaa
    15181 aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat gaagaacaag
    15241 cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca ctccatcctg
    15301 aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag aaattagaaa
    15361 cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg cacggaacgt
    15421 atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct gcccaagagc
    15481 gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat ctgcaagaat
    15541 tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta aaagagcaaa
    15601 aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac agctttattg
    15661 tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt cgggagatga
    15721 tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa gcaacagcaa
    15781 tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa gcacaagcac
    15841 gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc catggccgtc
    15901 cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga atccaagacc
    15961 cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc caatcaccaa
    16021 gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct gctgatatcg
    16081 atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg gctgaagaaa
    16141 aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt gccagtgaca
    16201 cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct gaggctgaat
    16261 tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt gttttaaaaa
    16321 caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat cctttgacag
    16381 acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca ggggcatacg
    16441 gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat tacagtatcg
    16501 taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa acaaaaaagc
    16561 ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt gaaaagatta
    16621 cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc cgggcgaaca
    16681 agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa agcaacaata
    16741 atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc aatcagggtc
    16801 tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc ctttagttca
    16861 acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag aataccaatg
    16921 gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata gactttgccg
    16981 tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac tgggtcattt
    17041 acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc ttttgttgca
    17101 actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt tgcgccattt
    17161 gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat cttattcaaa
    17221 attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc aatgatcatt
    17281 gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg caaaccgcct
    17341 actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc taatccagag
    17401 ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa catagcaggt
    17461 tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc ttctttttta
    17521 ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat atttgataaa
    17581 gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat atcgatggca
    17641 ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc gaatacggca
    17701 ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga gatccagctt
    17761 ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt tggtaacaat
    17821 gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg gaagaagatt
    17881 tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc ttgataacca
    17941 atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc ggctgtggaa
    18001 gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa aacgattcct
    18061 aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac tggaagaaaa
    18121 tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca aaacttcgag
    18181 tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac atttctgaat
    18241 ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat ttctgctaaa
    18301 acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt tccttgttct
    18361 ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt agaaacatcg
    18421 tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac gctaggtata
    18481 tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg catacgagtc
    18541 gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa cagcttctcg
    18601 gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg tctatgcaag
    18661 ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt attaaaaagt
    18721 acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa aaataggaac
    18781 attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg agtgagggaa
    18841 aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt ttagcgaaag
    18901 aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt tatggtgttt
    18961 cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat attcaaaaaa
    19021 aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct atttctggat
    19081 tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg atcatccata
    19141 cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg gagatcggtg
    19201 cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt atattagaca
    19261 ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca caaggatcga
    19321 tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa aaggagatca
    19381 cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat gacacttatg
    19441 cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag tattctgagt
    19501 cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga aaagtgtaaa
    19561 tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga taggatcaca
    19621 aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact gaaagaaaag
    19681 ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc atttagcggg
    19741 aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg tgaaccgtta
    19801 ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag caaaccaata
    19861 acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg aatagaagtt
    19921 cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc agtagatcaa
    19981 ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata tgaaaagatg
    20041 gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata cacaaagagc
    20101 ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg attgctattt
    20161 gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca ataaagggaa
    20221 cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag gtgtggttac
    20281 ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc agatcgcact
    20341 aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt atgtccatca
    20401 ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag aaaagcaact
    20461 gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg caatcatggc
    20521 aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca cttatttgac
    20581 gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc taaaaggaaa
    20641 attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa acacaaaaga
    20701 cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca gtgatcgaga
    20761 aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg atgagtatct
    20821 acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga cagatgaata
    20881 cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc ttcgaccaca
    20941 gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga tttacatcga
    21001 agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc ctcctggatt
    21061 aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata ttcgtacgac
    21121 gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg aactggaagc
    21181 aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag aagagatgct
    21241 ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc caaccgcaca
    21301 tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac gggcaggtat
    21361 gctttctgca cctttacgag atcgatttgg catcatttct catatggaat attatgacga
    21421 acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag agatcattga
    21481 agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg ctaatcgctt
    21541 gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg atcgtcctat
    21601 agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt atgttgacca
    21661 gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt taagtacgct
    21721 ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac cgtatctgat
    21781 ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt atgcctatga
    21841 acattttggt tacgactatc atatatgatt tttgctttta gattggagga aaatgaatga
    21901 gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg tgccaaacgc
    21961 aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc aatcgacaaa
    22021 cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat tgtgaggatg
    22081 cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag caagcattga
    22141 aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg ctttctgacc
    22201 cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc aagatttttg
    22261 aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat gctcgttttt
    22321 tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat gaagaaacac
    22381 cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta tattcttatc
    22441 gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg ttggaaaagc
    22501 aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt ttgggaaata
    22561 tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta gaaggaaggg
    22621 aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga aagacaccgc
    22681 accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat atggttgctc
    22741 ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg gatcaagaaa
    22801 atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc catttgtttt
    22861 taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat accaataact
    22921 ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat atttggattg
    22981 atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag gcgatgttag
    23041 agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa gtaacacttt
    23101 cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa tgaagaagct
    23161 tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa atgacactga
    23221 agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc aaaaagctgc
    23281 tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta gcaagtatga
    23341 aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga tgatccgcct
    23401 acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa aaataacaat
    23461 cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag atagaaaagc
    23521 acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc tttaagctga
    23581 tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt cggttgatta
    23641 gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct agccggggag
    23701 ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt tggtatgctt
    23761 atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt tgttaagttt
//
LOCUS       DACDNO010000045        23571 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDNO010000045 DACDNO010000000
VERSION     DACDNO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.045"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWI22_001847"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWI22_001847"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9169237.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWI22_001848"
     CDS             complement(1172..1867)
                     /locus_tag="IWI22_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9169238.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWI22_001849"
     CDS             complement(1880..2596)
                     /locus_tag="IWI22_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9169239.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3531
                     /locus_tag="IWI22_001850"
     CDS             2926..3531
                     /locus_tag="IWI22_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9169240.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3856..4179
                     /locus_tag="IWI22_001851"
     CDS             3856..4179
                     /locus_tag="IWI22_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9169241.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4361..5731
                     /locus_tag="IWI22_001852"
     CDS             4361..5731
                     /locus_tag="IWI22_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9169242.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5861..7162)
                     /locus_tag="IWI22_001853"
     CDS             complement(5861..7162)
                     /locus_tag="IWI22_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9169243.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7392..9383)
                     /locus_tag="IWI22_001854"
     CDS             complement(7392..9383)
                     /locus_tag="IWI22_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9169244.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9527..9793)
                     /locus_tag="IWI22_001855"
     CDS             complement(9527..9793)
                     /locus_tag="IWI22_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9169245.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IWI22_001856"
     CDS             complement(10303..12429)
                     /gene="pnp"
                     /locus_tag="IWI22_001856"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9169246.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IWI22_001857"
     CDS             complement(12617..12886)
                     /gene="rpsO"
                     /locus_tag="IWI22_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9169247.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13137..13700
                     /locus_tag="IWI22_001858"
     CDS             13137..13700
                     /locus_tag="IWI22_001858"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9169248.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13953..14474)
                     /locus_tag="IWI22_001859"
     CDS             complement(13953..14474)
                     /locus_tag="IWI22_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9169249.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14477..15319)
                     /locus_tag="IWI22_001860"
     CDS             complement(14477..15319)
                     /locus_tag="IWI22_001860"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9169250.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15312..16037)
                     /locus_tag="IWI22_001861"
     CDS             complement(15312..16037)
                     /locus_tag="IWI22_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9169251.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16213..16509
                     /locus_tag="IWI22_001862"
     CDS             16213..16509
                     /locus_tag="IWI22_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169252.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17077..18669
                     /locus_tag="IWI22_001863"
     CDS             17077..18669
                     /locus_tag="IWI22_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9169253.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18682..19422
                     /locus_tag="IWI22_001864"
     CDS             18682..19422
                     /locus_tag="IWI22_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9169254.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19590..20939
                     /locus_tag="IWI22_001865"
     CDS             19590..20939
                     /locus_tag="IWI22_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9169255.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21067..21657)
                     /locus_tag="IWI22_001866"
     CDS             complement(21067..21657)
                     /locus_tag="IWI22_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9169256.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21804..23156
                     /gene="brnQ"
                     /locus_tag="IWI22_001867"
     CDS             21804..23156
                     /gene="brnQ"
                     /locus_tag="IWI22_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9169257.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6750 a         4746 c         4490 g         7585 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 agcacaggtg aaggtggcga accagaagtg atcggttcaa cagaagaata atactgaaac
     3541 aatcaggtgg tgttagaagc ggcgggtcag cagcttttaa cacgcctttt ttatttctaa
     3601 tcgtttattt tcatatgggg cttccacttt tgtaaatggc tttacaaacg aaaaatccta
     3661 atttaccaag cttttcctcg catactttta tcgtaccttt ataaaaattt atactttgaa
     3721 aaaaagtatt caattattgt tgcatttttc ctgattcttt attagaatga accctcgttg
     3781 aagtaagtat ttctagttta tcctaggatt aattataaaa ataaaacatt cgttgattcg
     3841 aaggaggtta aacagatgtt tgaaagtttt gatagcagcc gcaaccgtta cgcttcagtt
     3901 ggtgtcgtat caagtttgcc agatgaattg attgacagta tatggtttat tattgaccta
     3961 gatcttaagg gagtcattcc attagaaaat atattaacct ttgatttgat cgaaaatcgt
     4021 ggaaaagtga cgatgcattt ttctcaagca ggaagcaatg tggaaatgga tattgatttg
     4081 ccgtttgcct attcgaacca atttcctgct caagtatacg cctatgatga cggtacgaga
     4141 gaaacgatcc ttttaccaag tgagattaga caatattaag gtgatagata aacgtggatg
     4201 tcccttattt tacagaccag gccaagttgt tgattctttg actcaattct tggcttcttt
     4261 ttatggagtt tcataaatta tcattgattt ctaattggta ttctcaggat aaacgctata
     4321 tactactaac taaattttaa agatataagg agtttaaaag atgcgcgatt taacaaaagg
     4381 aagccccgct aaactgattt tgatgttcac tgttccttta ctgatcggaa atgtttttca
     4441 gcagttctac aatatgatcg atatgatcat cgtgggtcag acgctaggta aaaatgcgct
     4501 ggcagcggtt ggtgcaactg gaagcctgac atttttgatc attggctttg cccaaggttt
     4561 aacagctggt ttagccatta ttaccgcaca acgttacggt gcaaaagatt atcggggtct
     4621 gaagaaaagt tttgctgcta gtgttgtcat tagtcttgtc gtcactgtga ttttaactgt
     4681 cctaagtctc ctgtttattc gtccgatgct tcaattgatg caaactcctc ctgaaatact
     4741 tgatcaagca caaacattta tttcaattat tttattaggg atctttgcat cagtcagttt
     4801 caatttgctt tctaatgtca ttcgcgcact tggtgacagc cgcacaccac tattctttct
     4861 gatcattgcg gtcatcatca acgtcgtact tgatttgatc tttattatct attttggcat
     4921 gggtgtggaa ggtgcagcta ttgctacagt aatcgcacaa gtttcttcca gcgtactgtg
     4981 tctagtgtat atcaagaaaa aaatgccttt gttacagtta cgcaaaaaag atttcaaatt
     5041 tgaaaaagaa gagtttgctg tacacctaaa tgctgctttg ccaatggcgt ttcaatcttc
     5101 gatcatcgct gttggtgcta tcgtgttaca agcagcacta aatagtctag ggacagatgt
     5161 cgtagctgct caagccgctg ctggtcgtat cgaccaattt gcaaatcagc cgatgatgtc
     5221 atttggtatt gcaatggcaa ctttcactgc acaaaactat ggtgcgaaag aatatggccg
     5281 aatcttgcaa ggggtacgtc aaactttact gatgagtgtc ggatttagtc tcttagcagg
     5341 tgctatcgtc attttcttcg gtcatttctt tgttcaattg tttgttagcc catcagaaac
     5401 acgtgtattt gaattagcac aaacctactt caatatcaat gggtctcttt actggatctt
     5461 agcagtcctg ttcattttac gctacacact tcaagggctt ggacaaagca agatccctac
     5521 gatcgccggt atgatggaat tgcttatgcg ttcttttgca gctatcgttc ttacaggaat
     5581 gtggggatac ccaggagcag cagcagcttc tcctttagct tggatcggtt ctgttgccgt
     5641 gttgctttac tcttatatgc gttcgatgaa acaattgaaa cacatgggtg aagaggaaca
     5701 tttcacatta gaacatgcag aagcaagata gccttgatta aaagaaatcg ttgcggctaa
     5761 aaatgaaaac ttttattgaa aatatcttct tcgtttaccg aaaacaggtt ttttagataa
     5821 caaaaaggct gtgacaaaaa atgtcacagc cttttttctt ttattctatt tttctttccg
     5881 tggactctac tttgaaataa tcgatatgtg catgctggta ttctaatacc gtgaaacgga
     5941 atgatgcaat ctcaatgatt gttcctgctt ttatttcttt atatcggcta gaagcaaagc
     6001 ccgcaagtgt agtaaaattc gtttcttcaa actctggaat ctctacatga aaataacgct
     6061 ggaaatcatc taatgttagt ttgccggaga tgatataaga gccatctgac tgttccgcta
     6121 tcaatgcttc gtccaaatct gtctcatctc ggatttcccc gacgatttct tctaaaatat
     6181 cttcaatcgt cactaagccg cttgtccctc cgtattcatc ttttaagata gcaatatgct
     6241 ttcccttttt tcgcatgatc gtcaataatt tttttatagg gatcgtttca gttgttacga
     6301 tcggctcttc aatcaactgt tctactgtca gctggtcatc tttataagat tgcttgatca
     6361 aatctggcaa ggtgacataa cctaaaacat tgtcttttga ttctaagata accggatagc
     6421 gagtatgtcc ttgttcaatc gaagctttta tcgcttcttt gacggtttct gaagcttcga
     6481 atacttgcat ggacgtacgg tctacttgta tatcttttgc caaggtatca tcaaattcaa
     6541 acacgttttc caaataatga tattcttctt tggtcaactc tcctttttca taagaattca
     6601 atgcgactaa tgtcagctct tcttgagaca atgtctcttc tccttcagat acgagacgca
     6661 cacctaatag cttccctagc cctgcagctg aatgattcaa tacccaaata aaaggaaaca
     6721 ttactttata aaaataatga agtggtttaa ctacgaacaa gacgaccttc tccgtattga
     6781 cgatactata gctcttagga agcagttcgc caataaccac atcgatatac gtaactaaaa
     6841 taaatcccag cgccacagag attgtactgc taatagccga actaaaaggt agtttaccga
     6901 tcactggttc tagtaaagcc gcgacagatc cttcaccgac ccacccgatg atcaaaccgg
     6961 ttagcgtaat tcctaattga ctagctgata aatagttatc taaatggtgt gtaacatgaa
     7021 gagctagctt ggcgttcttt acattttcct tttctagttg ttccaatctt gatcgtctga
     7081 ttttcactaa agcaaattca cttgctacaa aaaatgaagt aattgcaatc atcaaaaata
     7141 aaataatgaa attgataacc atattctccc gcctttctct ttttctattg tacccttttt
     7201 ctacaaaaag attgaaaata atgcgtattc atatttagga ataaaaaagg agatgcggca
     7261 tttttcagcc acatctcctt atcacttcaa aatcattgcg ttacttgttg ctaaacagct
     7321 ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta gttcaggtca
     7381 aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc aatgataagc
     7441 ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc tggcgaggta
     7501 gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt tcctagagtt
     7561 gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac ttctcgccct
     7621 ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat caaatcatgg
     7681 atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa gaacagattc
     7741 acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt atatggctca
     7801 tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg tttatctaaa
     7861 atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac caagaagacc
     7921 acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag ctgcgggata
     7981 taatctctca aagacacatc ctcggttgac gattcgcgaa taatgttccc ttgttcccaa
     8041 ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc gcctcggaag
     8101 aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa aaaggtaact
     8161 aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag cagtgctgtt
     8221 gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg atgagacgtt
     8281 cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac tgctgggata
     8341 tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag cagtttcaac
     8401 ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga agcaatgact
     8461 gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat catttggaag
     8521 aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt caacaaccac
     8581 gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca gctcagacga
     8641 atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc tgtcgtagcc
     8701 aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa aagcagctgg
     8761 atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa gttcattaat
     8821 cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc ttttccgaca
     8881 atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa agtgataatg
     8941 acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc tcgcaagcct
     9001 tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag taaggcagct
     9061 cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag tgtgtagagt
     9121 gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac atatttgatt
     9181 gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac aaaatcagga
     9241 ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta aagcggactt
     9301 gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat tgatatcttt
     9361 ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg atatccttta
     9421 ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct aaattgcttc
     9481 taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat tctttctttg
     9541 gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa attgcaccca
     9601 ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct agtaaaaatt
     9661 cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct cccgggactt
     9721 cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat aacgaccata
     9781 aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat tttacgcttt
     9841 attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa gttgtaacaa
     9901 aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca gaggttgtga
     9961 aatcagcggt ttgacctgag tattagagaa acaaacggaa aaatagttcc tcatattttg
    10021 ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg gagaggttgt
    10081 aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct cctaatgttt
    10141 ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt attcgaaaaa
    10201 aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta ttgtccccct
    10261 gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt cttcattttt
    10321 cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc cttgtttatc
    10381 gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt cgactttatt
    10441 gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt tgatcaagtt
    10501 gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt caccaacttt
    10561 gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt ctgcatcagc
    10621 tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag tttcatcgat
    10681 gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag caggttcgat
    10741 ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag cagcaatcgt
    10801 tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta atgcttcagt
    10861 taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag tgataccatc
    10921 ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc cttggatatc
    10981 agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac cggcgactgg
    11041 cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga tacttgcttg
    11101 ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat aaggaaattc
    11161 ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat gtccgatttc
    11221 ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag ggaaattgta
    11281 gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt ggtgctctcc
    11341 tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga acaaacctga
    11401 tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt catccaattt
    11461 acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat ctttttcaag
    11521 atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat gacctgcaaa
    11581 tttctcagca taagcttcgc ggatatcgat cttcacctgc tcgatattat cttcgcgagc
    11641 aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat caaagatttc
    11701 ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt tccctactgc
    11761 ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac caaacagcaa
    11821 tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca tattgatggc
    11881 ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt ctactgttgg
    11941 attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg ggccgtcaaa
    12001 tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca tagctggcga
    12061 gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac ggaagccttc
    12121 tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg cttccgtact
    12181 tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat acattttttc
    12241 ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag aggccacagc
    12301 tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg cttgttttgc
    12361 tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga atacttgttt
    12421 ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa acaaatgaaa
    12481 actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc atctagcttg
    12541 atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat gtgaatccca
    12601 ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg cggtaacgtt
    12661 gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact tttttcatta
    12721 ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc aagtggttga
    12781 tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct tcatggcgag
    12841 cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct ttcacctctt
    12901 tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa gtggcaggtc
    12961 atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc aagtagttaa
    13021 tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt ttgtggtaaa
    13081 ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca tattccatga
    13141 ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt gcaaaagaag
    13201 tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt gagttcttaa
    13261 aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt gttggcttag
    13321 ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca agtcctgacc
    13381 cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt ttgagccatt
    13441 ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat cgagaagtac
    13501 caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg aatggtgaaa
    13561 aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa atcgaccata
    13621 tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca ctaaaagaag
    13681 gcgtattggt gatcgagtag agaattggca ggttccttcg atactatgct acaagaaagc
    13741 tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt ctccactaat
    13801 cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta ttggatacgc
    13861 atcttttctt attttttctt atttcttcca tatgggtgtt tatctcagag atgatagaat
    13921 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    13981 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14041 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14101 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14161 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14221 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14281 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14341 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14401 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14461 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14521 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14581 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14641 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14701 ctcgatcgat catatcgatc ttatgacgta atgtttcaag agactcttct gttagttctt
    14761 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14821 atttggtcac aaccgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    14881 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    14941 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15001 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15061 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15121 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15181 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15241 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15301 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15361 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15421 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15481 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15541 actccatctt gaaacccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15601 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15661 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15721 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15781 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15841 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    15901 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    15961 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16021 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16081 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16141 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16201 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16261 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16321 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16381 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16441 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16501 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16561 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16621 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16681 aaaaccagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16741 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16801 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16861 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    16921 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    16981 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17041 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17101 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17161 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17221 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17281 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17341 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17401 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17461 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17521 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17581 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17641 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17701 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17761 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17821 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    17881 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    17941 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggtgc tggacttacc
    18001 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18061 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18121 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18181 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18241 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18301 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18361 acgatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18421 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18481 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18541 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18601 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18661 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18721 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18781 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18841 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    18901 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    18961 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19021 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19081 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19141 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19201 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19261 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19321 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19381 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggatcaata gctgattggg
    19441 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19501 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19561 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19621 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19681 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19741 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19801 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19861 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    19921 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    19981 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20041 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20101 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20161 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20221 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20281 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20341 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20401 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20461 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20521 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20581 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20641 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20701 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20761 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20821 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    20881 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    20941 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21001 ttcttaaatt tttgtgaaat ttcggcttat ttcttggaga taaaagcttc tgtcccagcc
    21061 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21121 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21181 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21241 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21301 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21361 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21421 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21481 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21541 gatgacccat aaagaatgaa agctagatgt attagggatc ttgatctcta atacaataac
    21601 tctcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21661 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21721 gttttatata gggaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21781 aaaaaaccgt aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21841 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    21901 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    21961 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22021 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22081 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22141 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22201 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22261 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22321 cactaggttc tgtaaatcaa gcgcctattc agccaaatta tcaagagcat gcttttttca
    22381 cagggtttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22441 tcattgtcac aacaatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22501 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22561 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22621 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22681 tattagcttg cttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22741 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22801 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctgtt ttgatgttca
    22861 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    22921 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    22981 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttatatgttg
    23041 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23101 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23161 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23221 ctcacatttt tgcataaatt tgacttaata ccgagaaact gacttttgaa caccgtttat
    23281 tgagaggtcg tgaaaaagcc gcttagtttc aagtattaag aaatattttg tgaaaatggc
    23341 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctggcttttg aacaccgttt
    23401 attgagcgtc tataaactga cctgagtatt agagaaacaa aacggcaaag tagctccata
    23461 tactttgccg ttttgttttg aactaatacc gaagagaagc tgatcaaaca acatttcttt
    23521 tataatatag attttagaca tatatatgat ataaaaatag ttttcgcaaa a
//
LOCUS       DACDNO010000046        23432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDNO010000046 DACDNO010000000
VERSION     DACDNO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.046"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(10..2154)
                     /locus_tag="IWI22_001868"
     CDS             complement(10..2154)
                     /locus_tag="IWI22_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9169258.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2511..3884)
                     /locus_tag="IWI22_001869"
     CDS             complement(2511..3884)
                     /locus_tag="IWI22_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9169259.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3884..4279)
                     /gene="rbsD"
                     /locus_tag="IWI22_001870"
     CDS             complement(3884..4279)
                     /gene="rbsD"
                     /locus_tag="IWI22_001870"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9169260.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4307..5224)
                     /gene="rbsK"
                     /locus_tag="IWI22_001871"
     CDS             complement(4307..5224)
                     /gene="rbsK"
                     /locus_tag="IWI22_001871"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9169261.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5333..6346)
                     /locus_tag="IWI22_001872"
     CDS             complement(5333..6346)
                     /locus_tag="IWI22_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9169262.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6427..7029)
                     /locus_tag="IWI22_001873"
     CDS             complement(6427..7029)
                     /locus_tag="IWI22_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9169263.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7214..7915
                     /locus_tag="IWI22_001874"
     CDS             7214..7915
                     /locus_tag="IWI22_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169264.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7925..10636
                     /locus_tag="IWI22_001875"
     CDS             7925..10636
                     /locus_tag="IWI22_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9169265.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11033..12661
                     /gene="treC"
                     /locus_tag="IWI22_001876"
     CDS             11033..12661
                     /gene="treC"
                     /locus_tag="IWI22_001876"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9169266.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12721..13902)
                     /locus_tag="IWI22_001877"
     CDS             complement(12721..13902)
                     /locus_tag="IWI22_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9169267.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14154..15680
                     /locus_tag="IWI22_001878"
     CDS             14154..15680
                     /locus_tag="IWI22_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9169268.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFAPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFRDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            complement(15749..16477)
                     /locus_tag="IWI22_001879"
     CDS             complement(15749..16477)
                     /locus_tag="IWI22_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9169269.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16490..17770)
                     /locus_tag="IWI22_001880"
     CDS             complement(16490..17770)
                     /locus_tag="IWI22_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9169270.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYAQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17911..18513)
                     /locus_tag="IWI22_001881"
     CDS             complement(17911..18513)
                     /locus_tag="IWI22_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9169271.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18759..18965
                     /locus_tag="IWI22_001882"
     CDS             18759..18965
                     /locus_tag="IWI22_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169272.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19278..19997
                     /locus_tag="IWI22_001883"
     CDS             19278..19997
                     /locus_tag="IWI22_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9169273.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20078..20761)
                     /locus_tag="IWI22_001884"
     CDS             complement(20078..20761)
                     /locus_tag="IWI22_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9169274.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            20993..23179
                     /locus_tag="IWI22_001885"
     CDS             20993..23179
                     /locus_tag="IWI22_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9169275.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7812 a         4210 c         4583 g         6827 t
ORIGIN      
        1 taatttattc tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa
       61 ggttagtaca ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct
      121 tgccgtaaaa ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt
      181 gttgacgcgt tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca
      241 tttgattggc acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt
      301 tgttgctgct tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc
      361 gttcaatttt tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc
      421 ttctttgtag tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt
      481 cagtccattt ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt
      541 acggaattgt gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa
      601 ggtcacgctg cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct
      661 tgataataag ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc
      721 gtagattcct tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg
      781 atcagttgtt tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga
      841 gccgcctgtc agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa
      901 atcaggcgtg aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg
      961 gataaattgt ccgttcccaa tatagatacc tacgtggtgg gtaacaccag aagaaggtcc
     1021 ccagaagacg agatcaccag gttgggcctg acttactgga atctgtgtac ctgcatattg
     1081 ctgttcgcct gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt
     1141 caaaccagaa cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag
     1201 atgcttgaat gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac
     1261 agcatcaatt ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc
     1321 gctttgattt gctgtatttg gacgagatga agcattgttc gttccgccat ttgatgaacc
     1381 attgtttgga tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt
     1441 tgtttgattc gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga
     1501 accattatta ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt
     1561 actagggact tcttcactag gatcagttgg tttctctgtt gaagtatccg gtacaggttc
     1621 aaattctgca gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt
     1681 tacttctttg gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat
     1741 agtgtaagtt accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc
     1801 caaaggttgc tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa
     1861 agaaggagca attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc
     1921 cggatcaatt tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc
     1981 ttctgtaaaa gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt
     2041 gactgtttga tcagctgttt gattatctac ggcaaatacg gtattggtcg acaaaacaga
     2101 cggagcaatc agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa
     2161 ctagtccttt tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg
     2221 aacagataat cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata
     2281 aaacaatttg taaataagca acgagcattc attcgttttt cattgacgtt cattttattg
     2341 catagaccac ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa
     2401 attataccaa agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa
     2461 aaagactgaa atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa
     2521 gacattaggt cttcatcaaa cccgctaacg agagtagtaa tcattgctac gactagacta
     2581 acagccagtc ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc
     2641 agactaccaa atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca
     2701 gcagctgcac caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa
     2761 acagttggtt cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa
     2821 cgagtttttg aacgtttact tcgtagaaat acacctaaag ctgcaccagc cattccaaaa
     2881 ttggctgctc ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc
     2941 atgatagggt ttacagccca ttggatacca aatattacca aaatattcca accaccagcg
     3001 atgattgcgc cgctgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa
     3061 ccttcgccaa ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata
     3121 actaatgaaa gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg
     3181 aatttttcaa caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta
     3241 tactgcatca gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa
     3301 agtaaagagg tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca
     3361 ttcactttaa acgttttggc ggcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg
     3421 gcattcgacg cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc
     3481 gtagtcgcta aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc
     3541 ggcgtaaaga tttccgatat caaaccaaat agtttttcga tcggatttag cttttcttta
     3601 ggagctgaat gatcgatttg acgcgttaat tccttgtaag caggagccaa tccctgatcc
     3661 atgattattt ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt
     3721 tttaattttt ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca
     3781 catggaagga cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc
     3841 tgctcggaca gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac
     3901 tcactaatat aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt
     3961 tattcaactc ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca
     4021 ataatttgat tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa
     4081 tcgattgtac ctcaagttct tctaaaacgt tttctaaaac agataaaaag cttggaagac
     4141 cgtttgagac agccaaatct attttttcac taccaaaggg gacaggcatg cctgcatccc
     4201 cgatacttaa tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga
     4261 ttacttttcc ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt
     4321 gttctaaagc ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag
     4381 aagaggctag atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg
     4441 tgctgcttaa tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt
     4501 taaaagcagg gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta
     4561 gtgtaatgat tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac
     4621 tgttatgatc tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa
     4681 tgatatctgt tttttcaagt agttcagtag ggatcgtttc acgagctggt gcaggattca
     4741 ggatcgttgt ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc
     4801 tactttcaaa ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga
     4861 cttcttgttc atggaaagca ttatttgcac ctgcatggat caagatacta ttttctcctg
     4921 ctgcgtctac cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag
     4981 atgtatcgat gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc
     5041 caacgcctcc aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttgattgg
     5101 cacctttacc tccgccagcg tgaaaaatct catgcgcatg cattgtttcg cctggcttgg
     5161 gcatgttggt taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt
     5221 tcattttaca aacctccaac tatctcattt attttgatgg gaaaaattat tttttaaaac
     5281 gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa agctatttca
     5341 agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct tcccattctt
     5401 tttctgggaa ttggatccga tctaacaata atttggcaga tgtttgtccc aattcgaaga
     5461 tcggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct atattatcat
     5521 agccaataat gctgtagtct tcaggaatgc tttttccaga ttcctctaat ccgcgatata
     5581 atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt
     5641 ctgctgtgat ttggtagcca ccttgttttg agaactgtgt aggtaagagg atcagttggt
     5701 cgtgagagat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga gcgtggacat
     5761 tttccggcgg attttcagga tagacaaggg cgatcgtttg atgacctaga ctaagcaagt
     5821 gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt
     5881 cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa ttttcgttga
     5941 ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct cgacgtgtga
     6001 gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat aatatcgtga
     6061 cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg aagaatggat
     6121 tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc atttgtctag
     6181 caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc tgaacagttt
     6241 tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg attgaaacgc
     6301 cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc atactcctat
     6361 ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa tcacgtgtgc
     6421 cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc gtactcgtgc
     6481 agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac gatcactgca
     6541 agcatcttca gcaagtacaa catgataccc atgttggaag gcatctaatg cagtagcata
     6601 tacaccgttt gttgtcgcta caccgcttaa aacgatagtt tcgattccac gccggcgaag
     6661 ttgaagatca agagaagtac caaaaaaggc acttggattg tattttgtga tagtcaacat
     6721 attgtttgta tctttcaagg aatccttcaa caacaagtca gtgaattctg ccggagcaga
     6781 caaagcagat attcctccag catctgtctg atgacttaga taagagaaag tagtgatgtc
     6841 tacattcact aaaactttca aggcagcggt acgtttcaat gtttttgcta acaaatcatt
     6901 gtttgtcaga atagattgtg cagtgtgtgg agagagtgta ccgttattta cgatcccttt
     6961 ttgcatgtcg atcgcgacaa aagcagtttt tgataaatca aaaatcatat tttcctttaa
     7021 atcattcatg atgttccttc tttcaaataa caatgttttt tatgtaagta aatcataaat
     7081 cgtttatttg tcaactaaat aaaggattcc tttctttagt ctagagaaat tcagtggtaa
     7141 aataaataag tatgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa
     7201 tggaggaatc agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg
     7261 agaaacgacc attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt
     7321 gattatactt ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat
     7381 ggatacacct gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga
     7441 taaacagctt actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct
     7501 cgttcctaat ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc
     7561 gcttgatcct gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc
     7621 gtcccaatta tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa
     7681 tcccaaactt cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca
     7741 agtattgaaa ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac
     7801 acataattct gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt
     7861 aaggtctgtt gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga
     7921 ttttatgaaa acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg
     7981 acgattcatt gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa
     8041 aactgctgga cctgtcatgc aaaaaacgat ggaccactat gttggtacag caggactttc
     8101 tgatttgcaa atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa
     8161 gatcccagat gcaaaagtag aaacaggaaa acagatctat tattccaata ctggtaaaaa
     8221 cgaagtgatt caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac
     8281 tgatggaaaa ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga
     8341 agggtataaa atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta
     8401 taccatcacg ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc
     8461 gaacgtcgga aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac
     8521 agatatttat tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag
     8581 caaagcttac aaagataaaa tggaacacaa tcaagagaag gcagaaaagt atttacaaga
     8641 tcgccctgaa gaacgcttag aagagctgaa aaaatcggca aacgaagaat tagaaccagc
     8701 acagcaaaaa ataacagaag gcaaagcaca aatagcacag ggaagaacac agttagaaga
     8761 tgctaaaaaa caattagcgc aacagtcagc agctatccag cagcttcctg aagcgcaacg
     8821 aacaatcgct gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca
     8881 attagttgag aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga
     8941 aaaaattgaa aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg
     9001 ttttcaagaa tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt
     9061 tttctttttc tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga
     9121 aaaccgcagg gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa
     9181 aaaatatgca atctatgcaa gtctagcctc agggataggc attgttttag gaacgatatt
     9241 aggaacgaat cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg
     9301 gagtgcgctt attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat
     9361 tgccgcattt ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc
     9421 attgcttcaa ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc
     9481 gctgtggtca cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa
     9541 gtcaagaatg cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg
     9601 cgtgggattg aaagattctc taagttctgt ctcagctaaa caatttggac cgatcattga
     9661 ctatcaagca atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga
     9721 tatgctgcag aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga
     9781 attgagaaaa aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc
     9841 tgatttggat cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa
     9901 aacaggaatc gctattacgc aaaaagcagc agaactattt gacttatcta caggagatca
     9961 tatttcgctt tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa
    10021 aaactatcta ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca
    10081 atcgatgatg gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga
    10141 aatgctttcc gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa
    10201 acaaatcgaa acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt
    10261 cgtattatca ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc
    10321 cgagcgtgta cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac
    10381 gatgtacatt gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg
    10441 tgtaggatat atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt
    10501 tccattagtg atcacatggt ggtcttatgt cttatcagca ggattaacta tcgtatttac
    10561 ggttattgtg atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt
    10621 gaaatcgaat gaataaaaac agaggttgtg aaatcagcgt tttgagctaa taacgagggt
    10681 cagctgctaa ttgaacaccg tgaatcagag gttgtgaaaa agctgtcctg catcgagcaa
    10741 aaggaatact caaataaaat tagctcctca tgttttacat ttgagaagac tgattgccgt
    10801 agataaaagc ttttgagcac catttataga agaaacaaga atattttgct attttaaaca
    10861 agaggataga caaatacatg tctgatctct tgttttttat tgggcaacag acctcttaga
    10921 atgtataaag ataaatttta caaacgcttt ctgttcagtt ataataataa atgataactt
    10981 gtatatataa gttgcggtag gtaataaatc agttgaatgg aggtaaatat ccatgacatt
    11041 taaagacaaa gtcatttacc aaatctatcc caaatcttac aaagacagta atggtgatgg
    11101 aattggagat ttaaaaggaa tcaaagaaaa actcccgtat ctgaatgatc taggcatcga
    11161 catgatttgg ctaaacccta tttatccaag ccctcaaaaa gacaacggtt atgatatctc
    11221 tgattacaca gccatcgatc ctatctttgg tacgatggag gaattcgaac agctaatagc
    11281 cgaagcaaag aaataccgga tagaaatcat gctcgatatg gtgctgaatc acgtttcgac
    11341 agaacatgaa tggttcaaaa aagcactagc aggggaccag cattatcagg acttcttcat
    11401 tttaagagaa gagccaacgg attgggtatc aaaatttggt ggaagtgctt ggtcgccatt
    11461 tggagatacg gggaaatatt atttgcactt atatgatcgg acacaagctg acttgaactg
    11521 gcggaaccaa gcagtaagag aggaactatt caaagttgtt cgtttttgga tggaaaaagg
    11581 ggtaaaagga ttccgtttcg atgttatcaa tgttatcggt aaagacgaac aattgaaaaa
    11641 taacaaagaa aatgatggca aacctgaata tacggacaaa ccaatcaccc atacgtatct
    11701 aaaagaattg aaccaagcaa cgtttgggac ggattccact atcatcacag tcggtgaaat
    11761 gagctctacg acgatcgaaa attgtatctt gtatacagaa cctagtagag aagaattatc
    11821 aatggtcttt aatttccatc atttgaaagt cgattatgaa aatgggaata aatggtctac
    11881 tcctccattt gattttgcag aattaaagcg actatttcat acatggggag aaaagatgag
    11941 tgagggtaac ggctggaatg ctctattttt taataatcat gatcagccac gtgcgctcaa
    12001 tcgatttgta gctatcgatc agtatcgaaa acaaggagca gagatgctag caaccatgat
    12061 ccatttgaac cgtgggacgc cctatattta tatgggagaa gaaatcggca tgatcgaccc
    12121 agactattca tcgatttccg actatattga cgtagaaagt ttgaatgctt ataagttact
    12181 ccttgaacaa ggattcacag aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga
    12241 taactctcga acacccatgc aatggtcaga cacagaacaa gcaggattta ctacagggaa
    12301 accttggtta aaagttggag gcaagctaga agagattaat gtagaaaaag aactatcttc
    12361 tgaagattct attttgtcct attacaagaa attgattcgg ctgagaaaaa catatccaat
    12421 cgttgcacaa ggagattatc atgcttatga agcaaatcat ccggaagttt atggctttct
    12481 tcgccaattt gaaggacagc agctgcttgt attaacaaat ttctatgata aagatacaag
    12541 gatcaccatc ccagaaggat tcacagaagc caatattctt atcgataatt attccgatac
    12601 tgttcttcat caaagtctgg agctaaaacc ataccaggca attgctttgc tgaaaaatta
    12661 aaacaaaaac acaaaacgtc gttcctacga gctattctcg taagatcgac gttttgtgtt
    12721 ttatttaaaa taatcttctt cagaaaatga agagttttca tactctcctt tatcgccata
    12781 aaatttattg tagcgttgtt tgaatttacg gaaaataaaa gtaacgagta atttgactaa
    12841 cgtatagata ggaataccaa aaataacgcc cacaattccc attagatcac ccatgacaag
    12901 taaaacgatt aagatcgtaa tcggatggat ctgtaaacga tcacccatta ctcgaggaat
    12961 caccaagtca ccgtggagaa gttgtacggc aaaccagaca atgacaaatt tgacagccat
    13021 aaaagtagag tcttggaatg caatgatcaa acctggaaag aaggcgataa acggcccaat
    13081 atatggaatg atacataaca acccagcaga aatagctaaa acactagcaa attctaatcc
    13141 gatcagtaag aaacaaaccc agtagactgc tccaaggata aatgaagcaa tgatttgccc
    13201 ctttagaaat gaacccaatt gttgattagc aatctttgtc aaatttttaa aatctgtacg
    13261 aaatgctggt gggacaatcg ctaaaactga ctgataaaat ttttgaggat ctctcaaaag
    13321 aaaaaaagcg acaattggac cagtaaataa agtaataaaa atagctgtaa cagttgaaaa
    13381 aatgttgctg atccgttgtg cgccactttc ccaataattt ccaataaatc cagctatatc
    13441 agacgtcacg tcgtcaatag aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt
    13501 ctctaaaaat tgtcggagac tctcttgaaa atcttgaaac aaatctggca aactactgat
    13561 caatgtcttt gcctgttctt caagaaaagg aacaagccat acgcttccaa gccccaataa
    13621 aaataaaata atcacataaa cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa
    13681 acgtttgatg actggttcca aaagataata caagatcagt gcaaacaata atgggggcaa
    13741 gatagtagcg ataatgattc cgacaggctt gaatataaac gagatctgat ggaaaaaata
    13801 aatgatcagc gcaagtaaga taactattcc taaaacatag taagaggttt ttcctcctaa
    13861 aaagcggatc aaacgattcg ttgtatctgt ttcttttttc actctttttc ctcctttatc
    13921 tataacttca ttatagacaa aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa
    13981 aatatcgtca atcattcata taaactttct catcaattaa aataagaaaa cgcttgacag
    14041 ttcttttaac catatgataa attaaatcct gttgctaaaa cgtaactatt acgttttgta
    14101 aaaggcgaat cggaacattc aaatgaaaga agatgagaag aggagtgtca taaatgaaac
    14161 gattgatagg tatattggcc atgctggttt tagcaggtat gttaactgct tgcggcgggg
    14221 caagtgggaa agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccta
    14281 tgtatgattt tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag
    14341 cagggactga ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata
    14401 cagatatctt tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa
    14461 cattacaaga aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg
    14521 gaagtgaaga aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc
    14581 tagacccaca tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg
    14641 atcagctgat tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct
    14701 ataccgagaa acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac
    14761 aaagatcttt tgtcacgcaa catgctgcat tcaactatct ggcattagaa tatggattga
    14821 atcaagtatc gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg
    14881 aactgaaaca ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca
    14941 aagatagtat tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc
    15001 ctttggaagg attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg
    15061 aagcaaacct cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt
    15121 tgacccccaa aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag
    15181 atcgctctct ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg
    15241 ggacttttgc tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag
    15301 aatataaaga atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg
    15361 agaaagagat ggcatttacg tttaaggatg gaacaacgaa acgttcaaat tatcgttatg
    15421 tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag
    15481 cagaagataa ggaaagtggc gcttttagat atattcaatt ccgtgatcac acgattgcac
    15541 cggctgactc cgaacatttt catttatatg caggaaatga aagtcaagaa gcattgctga
    15601 aagaaatgga gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac
    15661 aggaaatgct tttgcactga taggcaaaaa agaaccgaag ggtttggatc ggctagtcat
    15721 ttagtcggtc caaactattc ggttcttttt atgaaatcaa tttctttaat ccacggatag
    15781 aggctgggat ggtctcgccg tttgccaacg tgacttctct ggttttttta ttaatcgacc
    15841 gcacattttg tgtattgata acataggatt tatgacaacg gataaaattc cgatgcattt
    15901 tctcgatttc tttgatcttc tcatagaatt gtaattgggc atgagtcaaa tggacttcca
    15961 gtttatgtgg tgctcctagc gaagtagaaa aatattggat gtctttgatc ggtaaaaatt
    16021 tgatctcatc attaaactct attttgaaga tatctttttg gatctgggac atttgaaaat
    16081 cttcgaaaat tttgctcagg atatgctcga cattttcttt taaatcataa agatcttctt
    16141 tgacgatata atcagtcggt tcgatgttgc tttttaagat tttcatcgac atttccgtat
    16201 gggaagtcac atagattatt tttcctaagg gatctaattc acggatcaat ttaccgatcg
    16261 ctacaccatc catttcactg tcttctagtt cgatatctaa aaagtaaata ccaaaagaag
    16321 aagttctgat cttggctgca ttgagaagtt cttccggatc agaggtagaa atatggacat
    16381 aactgtctaa gttttcaatc atgattctat ttttgatcac cgttgataaa acttctaatt
    16441 gttttggatc gtcttcacaa atataaatgg gaatcatatc attactcctt tattttattt
    16501 gtatataaat cgtttgagaa aaacgctggt tttcaattga tgtttcaagg aaaagataag
    16561 gattggtctt tgtcaattca tccaagataa acaagccaag gccttcgttt tttggatttg
    16621 tttttgttga gaaccctttt tgtttcaact ggtttaacgg caatgtattt gttgttgtat
    16681 tgatcacacg aatcatcacg gaatgattct tcttgagtat ggtcaattcg atttttttct
    16741 tctttgcagc actagctcct tcaatcgcat tgtccaagat aataccaagc atacgtacaa
    16801 ggtttaccgt atgtttcgta tccagttcga ttggctcagg aaaatgaagc tggacgacca
    16861 accctttttc ctgtgccatc atgagtttta atgacagtaa actacggatc tcaggaactt
    16921 tcatattatc caacatactt agattggccg gaaaaaggtt catcatctgg cgtgtaggta
    16981 atatcgtttg agcataataa gaccgtaatc cttctaagtc gccttctgaa atatagctgt
    17041 gaagagaaaa aagcagattt tggtaatcat gtttgaattc tctcaagtct tgatagtttt
    17101 tttctagatc tttgatataa tcctgttgca tcctgacttg ttgctctaat aactgatcag
    17161 ctaatttctt attagagatt ttccaataat aaaccaagat gatcaggcca acacacagat
    17221 atagaatgaa aaaactcgta ttcaaagcta agacatcagg tgtttcacca gaaaaacggg
    17281 tataaaaaat aatgatatag tatgtaaata atatcaatgt cccgatcgta ccgatcatac
    17341 ggcggtccac agtgtgaaac caagtctcta acgatttttt aaaacaaaaa gctaataaag
    17401 cagataaaaa agcggataag gcaatgtgaa aatactgcaa tgaatctcct ggttcaataa
    17461 ccgaagattt caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg
    17521 tcaataaccc aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa
    17581 aagtaagcat cagcattcct atagcaatta gagcagtaaa aatccctaaa atcgaataga
    17641 cccatccaat caaaatacaa gaaatcagaa tgactcccgc aaacagatat ttgtatcgga
    17701 tgtgatggtc aatcaataag atacaaattg tgtaaatcaa atattgcaac gctatggacc
    17761 ataaaaacat aaagtacagc tcccattgat ttttaccaaa ttatagcaca attagcgtat
    17821 tattttttta atttttcagg gattttgaat tcagaactca taccaaagca gcagccagat
    17881 acaaaaaagt cagataagtt agttaattgt ttaatgattg tttttttcat gaaagattcc
    17941 tccaaaattc atcaagtagt ttgtaatagg aagcaacgta gctatttcta aaacaatccc
    18001 aagtagcaaa taggtagaaa agtaagaagc tgagaaaaat aaagtaatga gagaaaaaat
    18061 tagaaacatg ctgatcgcct tctttaataa tgggggacgt ttctcgggtt caatgggatt
    18121 gattgctgta cctcttgatc catagcgaag cagtaacaaa aagcagctga ttaacaaaaa
    18181 gaccatcatt gggaaggaga tagtcaattg cagatgtttc atcaccaagg gaacgcccca
    18241 taaataaagt agtgtccaca gcagacaaac gaattcagag tcggaatgca atccataagc
    18301 atatctgcgg atgcatatat aagaaagaaa ggtgattcct gtttccgccc atgagcctgt
    18361 caccagagaa aagactagaa cgaccgctag ttttccaaga ttattcaata aaacttctaa
    18421 ggcaaatttt acttggacat acaccatatc ttcttccgat ttgtctccgc tgaataaggc
    18481 ttctaataat ttgttcacaa ctttatcttt catctcatac gtacctctac cacaaatttt
    18541 atgctaaaaa aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga catcaaaacc
    18601 gtcatgattt cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt
    18661 ttgatacgaa aagtactttt tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa
    18721 aaattactga ccttataaaa ggtgatggag ggattcttat ggaaaatgaa caattgtttg
    18781 aagtcggcga aacatatgca tttaaatctg atttatttga atcaccaata aaaggaaaaa
    18841 tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca
    18901 atgataccgc tcataatctg cactggcagt tagttgtacg caaatcaagt acaatagaag
    18961 aataaaaaca gataaaatgt tgagagcata aagaggttgt gagtctccgt ttagctctga
    19021 gttacaagga ataatgttaa aaaacagctt ttcatgtttt atgaatattg ccacttgtga
    19081 ccgaggtgtt ggcaggagaa tactgtttat ttgaaaaaag ggctgcgaca agacttttgt
    19141 cacagccttt attttcatga ataggagtta cttttggcta cttttagaga agtagctttt
    19201 aaggagcaga taaagtcaag gcttttattt ctataaatga agtagtaaaa tgaagaacag
    19261 attttattac aggaaggatg ccgataattg tgatcattgt tgcaggaacg atcggtgcag
    19321 ggaaaagcac actgactgaa atgcttgccc aagatttaga aacaaaacct ttttatgaga
    19381 acgtggaaga taatgaagta ttgccgctat tttattctaa tccagaaaag tatacttttt
    19441 tattacagat tttctttttg aacaaacgct ttttggcaat caaagatgct ttcagccatg
    19501 atgacaatgt actagaccgc tccatctacg aagatagtat gctgtttcat ttgaatgcag
    19561 atttagggcg tgtttctgaa gtagaagtaa agcagtatga aggtctttta gaaacgatgc
    19621 tgaaggagtt agaagaaatt tcgccacaaa agaaaccaga tctactggtt tatattcgtg
    19681 tatcttttga aactatgtta gcgagaatca aaaaaagagg ccgggaatat gaacagctag
    19741 aacaagatcc tgaactctat tcctactata aagaactcaa tcgacgatac gaagagtggt
    19801 atgaacagtt tgatatatgt cccaaaatag tgatcgatgg ggacaaatat gatttcgtag
    19861 ccgatccggc atgtggagaa caaatcgtac aagaaatcaa gatgcgggca aagaaaatga
    19921 cagaagaggc agatgcccag tcgatccgct acactacaaa taacccgaaa gcaaagggaa
    19981 tctttacaac taaataatat aaaaactaag caaattgctc actcgcagat atttctctgt
    20041 gagcagcatc ttgcttagtt tttttgattc attgagatca aatagtgaac cgattcgtat
    20101 gataaaattg ccataaatga aatgtctgag tgagggccat gatcaaaaat aaaatggaaa
    20161 cgatcaaaat cgaatgggcg ccaaacagat gatgacagaa aacaaggaca atagctcctg
    20221 cgataaaggc aagaacgaca cttagatagt ggaaaaatcg atcaaaatct gctttttctt
    20281 tagaaataca ataatcatat aagttcgttg ctaccccttt taaatttcca gttgtaaata
    20341 aattagtgta ggcacgcccg ttgattttat caaaagctac ccactggatc gatgcagtaa
    20401 aagcaatcaa aatcgttact aatgcgggat gctcatggac gggtgtcaaa ctaacaaaga
    20461 aaaaaatcac tgcttcataa tatagaacaa acaagcgcca gaaatgtttt tctttttttc
    20521 taaaataatc aattagatac tttgttagaa gaatccctaa gaaaaagaaa agggtagaca
    20581 atactttttt accaacactt agccattctc cgtcaaatgc ttggatgatc gatagaatga
    20641 tattgccagt ttgagcagag gcaaaagcac catattggat ataagtataa ctgtccatgg
    20701 ctcctccgat aaatgtcagc aaaagtccta ttgtacgccc ctcgtgaaag gaagtctgca
    20761 tttttttcat caccttttat gaatattttt gtcttcctta tcttagcata aacgattgaa
    20821 aaaaaggaaa aattttcaga aaactaaaat catatcgctt cccattttca tttttagaaa
    20881 ggtctgttag tataaagaaa aaatagacaa cgaaacttta aataaccaac ttgaaataac
    20941 caacttgaaa taaacaaatt caaagaagaa agatgggaag gaaatcaatc agttgaatga
    21001 aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg gagaacaagt ataaaaagct
    21061 tcaacaagtc tttcggctac aagaagaaat cattgctacc tatgaagcgg cttatggcga
    21121 agcacccgga cttattgagg aagaagtaca agtaaaagaa agtgaaggaa acagctcttt
    21181 ctttaaaatg atggagaaac gttcctataa tcgcttcaat caagaacaaa aagcagctat
    21241 cacatatgat atgaagaaac atcttcgaat catagcagga gctgggagcg gcaaaacgca
    21301 aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa caagtagacg aagaacgcat
    21361 actaatgatc acttttacaa ggaacgctgc aaatgaacta aaaaaacgag tagataattt
    21421 cagccagaga aaaaccgctg tccatatcgg aacattccat agtatctttt ttaggatata
    21481 caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa ggaatacaag gagacttttc
    21541 aaaggatact gcacaaaaag tgaatgctgt tttgcaacag ctgatacgta aatataatct
    21601 ttatatattt gatcaatatg gagaaaaaac gattgcttct cggttagact attggcagaa
    21661 tatgaacctt tcagtagaag aaatggttca gttagttaaa gaaaaatatg attccattga
    21721 taagaatgca cgacagccga tatcagaacg actgtatgga ttgctcacgg aactccaaga
    21781 acaaaaaaga agccagcaat tgctagagtt caatgatgta ctccaaaatc tgaaaagcgc
    21841 tttggagaat gaggagatcc agcattatat cggccaaaaa tatgattatc tattcattga
    21901 tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg aaacggatga caaaggaaaa
    21961 caggatcaag ttgattgttg ttggagatga tgatcagagt atctatggat tcagaggatc
    22021 agagccagca tacatcaaga actttgaaaa agaatatcct accaaaactc ttttcttatt
    22081 aactaattat cgttctcggg cagctattgt ccaaggagca aatagattga tcagctataa
    22141 taaaaatgat cggataccaa aggcgatgat tccagcacaa aaagaagcag gtgttatgga
    22201 agcctatttg ttttctgata ctaaagcaga aagtcagtgg ctaatcaaac aaattcagac
    22261 atttattcaa aaaaatggga aatataagga atcaatcata ttataccgat cagcttcaca
    22321 aacgcagcag cttgtccaat ctctgctgaa aacgaatatt ccttttgtac tggaagcaga
    22381 tagtccatat gaaggtatct ttggtatcaa gtctttccaa catttctatc aaaaaattgt
    22441 tcattggcaa acagcactga atctgcaaaa gaaaaatcag gcctaccagc aattgctccg
    22501 gcagctgatg gcagactgct acctgaaaaa aagtgaatgc gatcaatact tttctcctaa
    22561 atatcaaaat atagccgttg cagattatat tttgtcgata agccccaatt taaaatcaaa
    22621 atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa acagaccagc atcaagaaat
    22681 ctattcgctt gttcaaagct atcttcatct gccacgtata gctaaggaaa tcgaccagag
    22741 tgatcaagaa tggatacgag aagaagtaaa gcagcatgct actttcagaa gactccaagc
    22801 cttatcgcaa gaaacccaag cgacagctaa agaattaaaa acccgcttga ttgcttacag
    22861 acaaggaaaa cttgatgcac tatgtatcca gagcattcac aaaagtaaag gtcttagtta
    22921 ccgaaatgtc tttttgattg gttgtaacga aggaagtttg ccttataacg gagcgacaga
    22981 aaagaaagta atcgacttta atgaaataaa agcagaacca gtgacgacga tagaagaaga
    23041 acgaagacta ttttatgttg cgatgactag agcaagaaat cgtctgtatc tatgtgtacc
    23101 tcaaatgaaa aattcaagaa aattgaaagt atcgagattt atcaaagaaa ccgggagtcg
    23161 tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga agtggtcagt ttcaagaaag
    23221 aagaaggaat gtacgaaaat tgcttctcaa atttttgtga aatttcagct tattttcgaa
    23281 gaagctattt ctggaacacc gtttattcgg aaaaaggagg tgtgacaaga attttgtcac
    23341 acctccctta tttattttga aaatgagaat actattatca aaatagagaa tatatagaat
    23401 aattctcatt attcaaacgt aaataatcag tt
//
LOCUS       DACDNO010000047        23174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDNO010000047 DACDNO010000000
VERSION     DACDNO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.047"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            141..214
                     /locus_tag="IWI22_001886"
     tRNA            141..214
                     /locus_tag="IWI22_001886"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Ile,seq:cat)
     gene            complement(727..1029)
                     /locus_tag="IWI22_001887"
     CDS             complement(727..1029)
                     /locus_tag="IWI22_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169276.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1278..1478)
                     /locus_tag="IWI22_001888"
     CDS             complement(1278..1478)
                     /locus_tag="IWI22_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9169277.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(1783..3015)
                     /locus_tag="IWI22_001889"
     CDS             complement(1783..3015)
                     /locus_tag="IWI22_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9169278.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3269..3838
                     /locus_tag="IWI22_001890"
     CDS             3269..3838
                     /locus_tag="IWI22_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9169279.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4016..4456)
                     /locus_tag="IWI22_001891"
     CDS             complement(4016..4456)
                     /locus_tag="IWI22_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9169280.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            4614..5378
                     /gene="map"
                     /locus_tag="IWI22_001892"
     CDS             4614..5378
                     /gene="map"
                     /locus_tag="IWI22_001892"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9169281.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5410..6333
                     /locus_tag="IWI22_001893"
     CDS             5410..6333
                     /locus_tag="IWI22_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9169282.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6409..7548
                     /locus_tag="IWI22_001894"
     CDS             6409..7548
                     /locus_tag="IWI22_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9169283.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            7541..8341
                     /locus_tag="IWI22_001895"
     CDS             7541..8341
                     /locus_tag="IWI22_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9169284.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8341..9168
                     /locus_tag="IWI22_001896"
     CDS             8341..9168
                     /locus_tag="IWI22_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9169285.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9146..9880
                     /locus_tag="IWI22_001897"
     CDS             9146..9880
                     /locus_tag="IWI22_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9169286.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9980..10846
                     /gene="rfbA"
                     /locus_tag="IWI22_001898"
     CDS             9980..10846
                     /gene="rfbA"
                     /locus_tag="IWI22_001898"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9169287.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10860..11432
                     /gene="rfbC"
                     /locus_tag="IWI22_001899"
     CDS             10860..11432
                     /gene="rfbC"
                     /locus_tag="IWI22_001899"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9169288.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11454..12482
                     /gene="rfbB"
                     /locus_tag="IWI22_001900"
     CDS             11454..12482
                     /gene="rfbB"
                     /locus_tag="IWI22_001900"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9169289.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            12580..13431
                     /gene="rfbD"
                     /locus_tag="IWI22_001901"
     CDS             12580..13431
                     /gene="rfbD"
                     /locus_tag="IWI22_001901"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9169290.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13466..15499
                     /locus_tag="IWI22_001902"
     CDS             13466..15499
                     /locus_tag="IWI22_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169291.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            15543..16823
                     /locus_tag="IWI22_001903"
     CDS             15543..16823
                     /locus_tag="IWI22_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9169292.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17033..17839
                     /locus_tag="IWI22_001904"
     CDS             17033..17839
                     /locus_tag="IWI22_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9169293.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17851..19071
                     /locus_tag="IWI22_001905"
     CDS             17851..19071
                     /locus_tag="IWI22_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169294.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSNGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19061..20647
                     /locus_tag="IWI22_001906"
     CDS             19061..20647
                     /locus_tag="IWI22_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9169295.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            20686..22824
                     /locus_tag="IWI22_001907"
     CDS             20686..22824
                     /locus_tag="IWI22_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9169296.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(22892..>23174)
                     /locus_tag="IWI22_001908"
     CDS             complement(22892..>23174)
                     /locus_tag="IWI22_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9169297.1"
                     /translation="EFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPK
                     SMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         7311 a         3919 c         4764 g         7180 t
ORIGIN      
        1 aaaatcattc gataaaatag tgatgttatc gcatatcttc actatcaccc ataatagtca
       61 cactccaagc tatgcgataa caggtttgtt gccacacatt ctactggttg attgtttatg
      121 gctttatgtg gcaacaacca gtacccttag ctcagttggt cagagcagac ggctcataac
      181 cgtccggtcg taggttcgag tcctacaggg tacattaacg tagccatttg aatcgttctg
      241 tgttagaatt ttttgaagag tattatacaa gctaaagctt ttcttcattg ccactcaaat
      301 gagtggcttt tttatgtatc cttttatgga ttaatgaaag gatgtttcac atagttatat
      361 ttctgtatat ttgaaaagtt ttactttgat ttttaaatag aaagacattt gggttaaatt
      421 gtgagatgat aataaagaag agtttaaagc gcaccccaag ccacttcccc ataagtgtgt
      481 tacgctttaa actcttttat atttgaagct attaaaaggc atgccatatt tttgaaaaaa
      541 agtgagaaaa aaggcttaca attggagtgg tagttaatta gtgacttatt tttgatttta
      601 tagcactgat actataaaat atagatatca tcatattaca caatcttaat actaacttaa
      661 aaaatatctc ctttcacaag tatggtgata aaattcgttc cgggctacct ttttaggtag
      721 cctactttaa tctttatacc tttctggatc aacgaaagta tactttatat agtcataacg
      781 ccgatgatcg ctacgtgcgt ccggcacgtc agtcacgata tcaaacaaaa agtatacatc
      841 tttcttcatt ctagtttttg cagcagggat tttaaagtag ttcttattag aatagtagag
      901 attgattaat aagctatctt cgattgctaa aaagaaaact tctgaattcc atactttata
      961 aaaatctttg ataaatctat tcgaagggtc aaatttaaac cataattgtg tttttccttc
     1021 catcagcata aaagttcacc tcaaaaagag tatacgaacg aatgttcttt ttgtgaaggc
     1081 ggaatttaat atactgaata atagaaaaca agtacttgtg ccaaattatg tgccaaaaaa
     1141 attcgaattc aacaaaatgt aaacaaatag aaaaccttct atattaagat ttcctgcaat
     1201 aacaaacaca acagaagacg tgtaatagtt agtcaggaac gtacaaataa cccctgtatc
     1261 ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa actgctatgt
     1321 cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat agatgcatgc
     1381 gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt gattactaaa
     1441 gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat tcgaattaaa
     1501 cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc ttaactctag
     1561 cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg actttttatt
     1621 atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag taccatataa
     1681 aaaaacggtg gggttgttta gcgaaataaa ggatctaagc caatagctta gatcctttat
     1741 tctatgcttt atattatttt ttgcatgtgt gcgaaagtat actcaagccc aatcgccatt
     1801 tcggaaaatc ggtactgtag agccatcttc acggattccg tcgatatcca tcttgtcaga
     1861 tcctaccatg aaatctacgt gggtttgact tctattcaat ccagcttctg ctaattcttc
     1921 atcggacatt tctgtaccgc ctttcacact gaaggcataa gcagaaccta gtgctaagtg
     1981 attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg atgccagatt gagaaatagg
     2041 tgaagggtca ggtacaagtg cgacttctcc taaacgacgc gcaccttcat ctgtatcaag
     2101 cagttttgct aagacatctt ctccttgttc ggcagaaaaa tcaacaactt ttccatcttt
     2161 aaaagtaaat ttcatacccg agatgatggt gccagcataa ctcagtggct ttgtacttga
     2221 aatgtaacca tctactcgat gactgtcagg tgctgtgaac acttcttccg tcggcatatt
     2281 tgccatgaat ttttctccac gagcattata gcttccagcg ccttcccaaa gatggttttt
     2341 aggcaatccg ataatgatat ctgtaccggg agcagtatag tgaagggcag aaaattgttc
     2401 tcgattcaat tcttctgctt tttttgctaa tttttcatca tgcttcttcc atgctaagac
     2461 agggtcttct tcatagacac gagtcgtttt gaaaatctga tcccataatg cctcaacttg
     2521 tttttcttct ggcagttctg ggaagacttt tgctgcccat tgtttaccag ccgcagccac
     2581 gacagtccaa ctgactttat ttgcttgagt tgccttgcgt aaattcatta gtgctttgcc
     2641 attcgctgat tgataagacg ctactcgatg gctatctaca ccagcaaatg catcgggatc
     2701 ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc cattcatccg cttgatcgat
     2761 tttagattgt ggtacattct caatacgatc cgttgcagca tgtaaaagaa attctctttg
     2821 gatctggtcg tcagtccatt gaacgataac ctcagcggct cctagtttat aagcttcttg
     2881 tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc tgtaaaacaa ctgtgtgtcc
     2941 tttttctgtg gctacgccag tttctgcaat taagcgtgca tattttttta gaagttcatt
     3001 aaaatcagat aacataataa aaattcctcc tcaccatttt ttctggtaac tattagcatg
     3061 ataccattta attatgattc aaacaatgag gctttaaata aaaaaaggag gttatttttt
     3121 taaggttaag tttcatgata tatgttacta cttgctattt cagcttgtga agaaaatcaa
     3181 aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa ttaatcaatg gtggaagcgt
     3241 tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa aagacgatta ctagagaaca
     3301 gattttggca gctgcttatg aagtagctgc caccgatgga tttacacgat ttactgcgag
     3361 aaacatcgcg aacaaaatga attgttccac gcaaccgatt tatcttgagt ttaaaaatat
     3421 ggatgacttg agacaagctc tttttgagca gatctatgat catttacaat atgaagtgtt
     3481 cactgttgag catacaggga atacgattat cgatcttgct tttaattata tccattttgc
     3541 aagaagagag aagaagttgt atcgttcact gtacttagaa gaatatggtg gaggaaaaga
     3601 gatgcagaat ttctcttata cttactttgt gaatacagtg aaacgcgatc cagcttatgt
     3661 gaatctttca gaagagcaga tcgaatcttt gtataatggg acatggatca tagctactgg
     3721 gatcgcagct ttgatgtctt caagcatcat ccatccaact gatgaacaga ttgaaaagat
     3781 gatccaagac agtatagatg ctatcttgaa attcaaagaa ccaatcgaaa tcgattgaat
     3841 atactcaaaa gttccacaaa tcagtaggta acaagagatg tgtcaaaata aggaaaagtt
     3901 cgtttttctt acagcgtatg catttttaac tcccgaagaa ctcttccatg aatgcaatga
     3961 ataaaagagc catacagatt gaataaatct ggatggctct tttattgcaa gtatattaaa
     4021 tttttgcgac tagttcttta gcgaaagctt ctaaattttc gatatcttct tcttcagcat
     4081 tcagatccac tttaacattt tcggctcctt ttgatgcgcc tgtttttgca aaagcagcat
     4141 cgaaatcatc caccgatttg cagaattcat cgtaaaaagt atcaccagat ccgcagacac
     4201 cgtaaatttt tcccaataga tccaattctt gcaagtcttc ataaaagtct acgatctcat
     4261 caggcagatc gccatcatcg tatgtataag tagctactac gcaaatatct gcctcctcga
     4321 attcatctgc atctacttgg gtacattcgt tgatttcgac ttcgatatcc agattttcaa
     4381 atgcttctgc aacaatatcg gcgatttctt cagtgtttcc tgtcatgcta gcatatacga
     4441 tttttgctaa agccatgatg tgcctcctcg tttataatta tttaacaatt atgaattata
     4501 ccaaaacatc attgaaaaga aaataaaacc taaagacacc tggggaatgt catttggcta
     4561 aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa aggagacgaa aatatgatta
     4621 ctttaaaatc agcacgtgaa attgaaatga tggcagaatc tggagcttta ttagcagatg
     4681 tacataaaaa tctgcgtgat tttataaaac caggaatcac aagttgggat attgaggtat
     4741 ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc gcaaattggc tttgaagggt
     4801 atgaatatgc gacttgtatc agtataaatg atgaaatttg tcatggattc cctcgtaaaa
     4861 agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg tatcgatttg aaaggcggca
     4921 tttctgattc ttgttgggcc tatgtagttg gagaatctac acctgagatc gaccgattga
     4981 tggaagtaac gaagaaagct ttgtacatcg gtattgagca agcacaagta gggaatcgga
     5041 ttggtgacat cggtcatgct atccaaactt atgtagaagg cgaaaatcta gcgatcgttc
     5101 gtgattttat tggtcatggc gttggtccta cgattcatga agaaccagca gttcctcatt
     5161 acggagaagc tggaaaaggg ttgcggttga aagaaggcat ggttatcacg atcgaaccta
     5221 tggttaatac cggaacttgg aaaatgaaaa tggatcctaa cggctggact gcgtatacaa
     5281 gagacggcgg actttcttgc caatatgaac atacattggc tattactaaa gatggtccaa
     5341 gaattttgac atcacaagga gaagaaggca cttattgatc tatccagtca ataggtacgg
     5401 gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg aaaatctgat gcgctttatt
     5461 gagacgactc aaaagcgcat cactgattca gaaatgagta cgacctctgt cgtcgttgct
     5521 tattatttac tcttgtcttt atttccattg attattgctt ttggtaatat tttaccgttt
     5581 ctccacatcg atcaagagac tgtactgacc tatttacgag aagtgattcc tgagaccgtt
     5641 taccagttta tcggtcctgc aatcaaagac cttttgacac aaagttcagg aggcttgtta
     5701 tccgtttctg cattagcggc tttatggtct gcaagtcaaa gtatcaatgc tttgcaaatt
     5761 gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt ttttgatcgt ccgtttcttc
     5821 tcactaattg tcattgtctt gttcatgatt gctattagtg gggtaactat cgttcttggg
     5881 ttgggacagc tgattttgga tgctattcag ccaatcgtac aaatacccgt gaactttatc
     5941 gatcagttcc aaactttgaa gtggccaatc acattggtag ctttatttgt catcatgttc
     6001 ttgatttatt taattgtccc taatgctcag ttgaagttga agacgatcat tccgggcacg
     6061 atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt ttagtatcta tgctaggtat
     6121 tttgcaacga ggatcagcgg ttatcagatc ataggaagtt ttattgtact catgctttgg
     6181 cttaattttg ctgctacgat catcattttg ggcggaatca tcaatgcagt tgttcaagaa
     6241 tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa gccgaaaatg gttcaataaa
     6301 ttgaaaacta aattttctca taaaaaaaag tagaaacaag agttcttatt gtaaatttat
     6361 atcagtaatt tataataatt atgactctta attaaagtag gtgcccatat gcttgtttct
     6421 tttagtatgg ttataagatt ttttctgaca attttattat cactgatatt aacacctgta
     6481 ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac caaatgaaag aagaataaat
     6541 aaagttccaa tgccttcagc gggcggactg cctatcttta tttcatttgt tatatcgact
     6601 ttattcttat tccgtaaaat tatccctcaa tcgtatattg taccgatctt gattgcttct
     6661 ttagtaatca tagtcacagg agtgctcgat gataaatatg agctgacacc taaacaaaaa
     6721 agtataggga tcttgattgc ttctcttatt atttattttg tagcagatat acgtattgat
     6781 tccttcacgc ttccttttat tggatatatc caactaggct ggcttagttt tcccgtaacg
     6841 attttttgga tcttcggtat cacgaatgcg gtcaatctga ttgacggttt ggatgggctg
     6901 gcggcaggga tttcattgat cggtttaatt acgataggaa tcattggata ttttttcctc
     6961 catgcttcta cagtgtatat tccagttgtt attttctgtc ttgtagcaag tattatcggt
     7021 ttttttcctt acaattttta tccggcaaag atttatttag gcgatacagg agctctattc
     7081 ctagggttta tgatggccgt cctttcacta caagggctga agaacgtgac ttttgtttca
     7141 tcgatttctt tgctgatcgt catgggagtg ccggtaacag acacgttttt tgctatcatt
     7201 aggagaaaag caaaccgtgt ttctttttct acagcagata aaaaacatct tcatcaccga
     7261 ctaatcagtt taggctttac tcataaaggt gctgtcctga cgatttatgc gatggctttg
     7321 atgttttctt tcacagctat ggtcatgaat tatactggaa gaatcggaac gatcatattg
     7381 attattgcca tgttgtttgc tgcaatttta ttatttgaat tgattggtct gatcaatgag
     7441 aaacatcagt ttattttaaa aacattgcga tttttaggga ataaagagta ccgtacgcaa
     7501 gtaaaacaga aatatttgaa gaaatggaac cgtcatatag atgaataaca aattaaacca
     7561 gccgatcctt attagtattg tgacggcaaa cagtgataaa atttttcgga cactagataa
     7621 ttttatttca actgtaaaag atgaaacagc agtaaaaatc cgaatttttg acaacaattc
     7681 acttcccgag tacaaaaaaa gactaacaga gtataggaaa tatccctaca tcgatatcta
     7741 ttttcatgat gaaaattccg gatttggtta tggacataat cacaatctga tagctagcga
     7801 ttctcgctat ggcattattt gtaacccaga tattttagtg gatgaagaga cagttacagc
     7861 attggtcttg ttattgaaag aacatccgga atgtgcgatg ttagcaccta agattcttaa
     7921 tgaagatgga acaactcagc acctcatccg tgagcagctg gctgtttttg attacgtttt
     7981 gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac aaacgactgg ctgtgtttga
     8041 atgccgagga ttgcctactg atgctaatag ttatgtacgg atgatctcag gtagttttat
     8101 ggtagctgat attgataaat ttaaagagat aaaggggttt gatgagcgtt tcttcatgta
     8161 ctttgaagac aacgacttgt gtttgagttt tgaaaaagca ggttacaagt tattgtacac
     8221 acccttttat gcagttatcc atttttacgg gaagggtgcg catcgaaatg caaaactgtt
     8281 tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag tgggggtggc gttttttctg
     8341 atgaaaccag aaatattagt agtcgttgtg ctttatcagc aactattcag tcaatctcca
     8401 gcttatggac cattaagcaa agcacttagc gaaaagcaaa ttcagcttat tatttacgat
     8461 aatagtcctc aaaggcagca aaatgagctg tttgaaaaag aaaatatcgt ttatcatcat
     8521 gatccgcaaa atcctggttt agcggtggct tataattttg cgttgtctca agcagatgaa
     8581 ggaacacgtt gtctcgtcac tctggatcag gatacgaggc tagtcgacga ttattttgaa
     8641 ctattgcgta aagtaacctt tacagatgaa tgtgttgctg cagttccgat gattttttct
     8701 gggagccgac aagtttctcc cctatattca gatcgttata tcaatcgtac agctcaagca
     8761 gtagaagttg aaggcccaac agctgaacga atcatggcaa tcaattcagg tgttgcctgg
     8821 tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg ctttttcttt agattttttg
     8881 gatcattggc tgttttggaa agtggatcag ttggaaaaaa ctgtggagat ccttcctgta
     8941 cgaatggaac atgatctttc ggttttagac tatgaaaaag tttcaagtaa tcgataccgt
     9001 tctattttac aagcagaaaa tcgattttat catgaatatg atcaaaggca tttatccagt
     9061 caccgacgac aattattgtt aagaacagct aaacagttct tgaccgtcaa aaaccggcat
     9121 atctggcgga tgactttgcg ctcatatgtt aagaattgga aggtgtgaat tcgtgcgttc
     9181 ggtttgtata gctaccttta atggaggcag atatttaaga gagcagttag atagtatttt
     9241 atctcagctt tcagcagaag atgaagtagt catttctgat gatgggtcag tagatgatac
     9301 atgggatata ttaactgaat acgcagaaaa agataagcga atccagcttt tttcggggcc
     9361 tcgtcaagga ttgatcgcta attttggtta tgcgatccag cagacgaaag gagatctaat
     9421 ctttttggca gaccaagatg atgtttggtt ggagacaaaa gtgaaaaaga tcactgacta
     9481 ttttgagcag aatccggatc agctagtagt agtttctgat ttaatcattg tggacagcga
     9541 gctgcaagag atccatgatt cgtatttttc gtttcgaaat tccaaagaag gatggcttaa
     9601 taatgtgata agaagtcact atattggtgc tggtatggct ttccggtctt ctttaaaacc
     9661 cgatattttg ccgatcccgt cagaagtgcc gatgcatgat atgtggattg gtttgctggg
     9721 aggaaagaag actgggttcc taagagaacc gttaactctg taccgaagac atgatttaaa
     9781 cgcgagtgag attgaaacac attctaaatt acggcaaaaa atttcttggc gtgttcatct
     9841 gattacggcg ttgatcaaac gaaaatggtt taaacgttga taaaagatgt ttaaatcctt
     9901 aatatttcca tgacaaatat aacaattgat gagataataa caaagtgaaa tttgaaatca
     9961 aaaaggagag cagatatcca tgaagggaat cattcttgct gggggcagcg gaacacgtct
    10021 gtacccttta acaaaggcaa catcgaaaca attgatgccg atttatgaca aaccaatgat
    10081 ttattatcca atgtctactc tgatgttggc gggaatcaaa gaaattctga ttatttcaac
    10141 gccacaagat acaccacgtt tcaaggaatt atttggaaac ggaaatgatt taggactgca
    10201 gatcgaatat gcagtacaag aaagtccaga tggattggca caagcgttta tcattggaga
    10261 agaatttatt ggagatgata gtgtctgctt ggtattaggg gacaatattt actatggtgg
    10321 cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat acaggcgcga cagtatttgg
    10381 ctatcatgtc aatgatccag aacgctttgg tgtagtagaa tttgatgaga atatgcaagc
    10441 tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat tacgctgtaa ctggccttta
    10501 tttttatgac aatgaggtaa tttctatcgc taaaggtatc aaaccttcag aacgtggaga
    10561 actagagatt acggatgtca ataaagcata tttggataaa ggaaaactat ccgtagaact
    10621 tatgggacgc ggatttgctt ggcttgatac aggaacacat gaatcattgc ttgaagcatc
    10681 tacctttatc gaaacaattg aaaaacgtca gaacttaaaa gtggcttgtt tggaagaaat
    10741 tgcttaccgt atgggctaca tcgataagga acaattgctg gcccttgcac agccattgaa
    10801 aaaaaatcaa tacggtcaat atttattgaa tttggccgct gaataaggag tccttacaga
    10861 tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt agaaatggac gtctttggcg
    10921 atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa atttgcagaa catggattga
    10981 aatatgactt tgttcaagac aaccattcac tatcagctga agctggtgtc cttcgggggc
    11041 ttcatttcca aaaaggagaa gctgcacaga caaaattgat tcgggtagtc actggagcag
    11101 ttttagatgt agtagtggat atccgcaaag gtagcccgac ttatggtcaa tgggacggat
    11161 ttatcttatc tgaacataat catcgacagt tgcttgtacc taaaggatat gcccatggat
    11221 tcgttacttt gacaccaaat gtgaacttcc tttataagtg tgataattac tataatgcag
    11281 aagcagatgg gggcatcgca tttgatgatc cagatttagc gattgattgg ccaattgatc
    11341 catcaagagc gattatgtcg gaaaaagatc accgtcatcc gacattaaaa gaatttgaag
    11401 cagaaaatcc atttgtttac ggagaaatct aagaattaag gagaaatttt cttatgaaaa
    11461 acatcattgt taccggaggt gccggattca tcggctctaa tttcgttcat tacgtggtaa
    11521 acaatcatcc agaagtgcat gtcactgttt tggataaact gacttatgca ggaaacaaag
    11581 agaacttggc cggacttcct agcgatcgcg tagaattggt cgtaggagat gttgcagatg
    11641 cagaattagt ggaccgtcta gtaaaagaag cggacgcagt tgttcattac gcggcagaat
    11701 cccataacga taattcatta aaagatcctt tcccatttgt acaaacgaat atcattggta
    11761 cgtatacact tttagaagct tgccgcaagt atgatgtacg ctatcatcac gtatctactg
    11821 acgaggttta cggcgatctt ccattacgtg aagacctgcc aggacatggg gaaggcgaag
    11881 gagaaaaatt cactgcggaa acaccatata atccttcaag tccatattct tctacaaaag
    11941 ctggttcaga tctgttagta aaagcatggg tgcgttcttt tggcttacgt gcaaccattt
    12001 caaactgttc aaacaactat ggtccttatc agcatattga aaaatttatt cctcgccaag
    12061 taacgagtat cttaagcggt attcgtccaa aattatatgg cgaaggaaag aatgttcgtg
    12121 actggatcca tacgaatgat cattcttcag cagtgtggct gattttgacg aaaggcagaa
    12181 ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga taataagacc gtgatggaat
    12241 tgatcttaga aatgatgggc caaccaaaag acgcatacga ccatgtaaac gaccgcgcag
    12301 ggcacgattt gcgttatgca atcgattcga caaaattgcg agaagagcta ggttggcagc
    12361 ctgagtttac gaacttccgt gatgggttgg ctgacacgat caaatggtac gaagaacatg
    12421 aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc atatgccaaa aatggacaat
    12481 aatacagagg aaatttcact gtattaatgg aaaaacacat gtgaggcagg cgctcgcctg
    12541 tctctttttt aaaataaaaa tcaaggagcg gataaccata tgtttttatt aactggagga
    12601 aatggacaat taggtacaga attgcgccat ttattagatg aaaaaaatct gccatatgtt
    12661 tcaacagatg caaaagactt ggatatcaca gatgctgaga aaacaatggc ttatattaca
    12721 gaattaaaac ccgatgttat tttccattgc gcagcttaca cagcagtaga taaagcagaa
    12781 gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg gcacaagaaa tgtagcactt
    12841 gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta cagactatgt tttcgatgga
    12901 aaaaacaaaa atgtagaata cgaagtagac gaccaaacga atccactaaa tgaatatggt
    12961 cgtacgaaat tattaggcga aaaagcagta gaagagattt tagaagacta ttatatcata
    13021 cgaacttctt gggttttcgg gatctatgga cacaatttcg tcttcacgat gcaacgccta
    13081 gcggaaaccc gcgacaaact gactgtagta aatgaccagt ttgggcgacc aacttggaca
    13141 cgtactttgg cagaatttat ggtatatgtc atcgaagaaa aagcaccatt tggtatttat
    13201 catttgtcga acgaaaatag ctgttcttgg tatgagttcg caaaagaaat attgaaagat
    13261 acagatgtag aagttgcacc tgttacgtca gaagaatttc cgcaaaaagc aacacgtccg
    13321 cagtattcag tcatgagcct gaaaaagaca gaagcgttag gttttgtcat accaacttgg
    13381 caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa tgaataagta atttcctttt
    13441 tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga
    13501 aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt gacctgtttc taaaactccg
    13561 ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc
    13621 acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat
    13681 ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct
    13741 ctcacaagct gaagaggact tttcgttggt gaacgaggat tattcattaa acggacaaaa
    13801 catgatcatt gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa
    13861 ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt
    13921 agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga ggaaaaaagc
    13981 tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat
    14041 gcaacatgtg ggcgatttga ttttctttac tttgggactg atgattgctt tttatcatta
    14101 tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt
    14161 cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct
    14221 gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt
    14281 ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg
    14341 gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt
    14401 aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat
    14461 tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt
    14521 tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa
    14581 gttactggga cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg
    14641 gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat
    14701 ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag
    14761 agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta
    14821 tttctatgcg attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc
    14881 agttgctgtg ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt
    14941 ttacctcgct tttgtcgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga
    15001 aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca
    15061 agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg
    15121 agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga
    15181 tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc
    15241 taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt
    15301 tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta
    15361 caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat
    15421 gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa
    15481 tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta gtagaaagga
    15541 gagtgaagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct
    15601 tgcttttgtt gctgacgcta gtcttttttg tttggacagc aggggtaggt tttccagcaa
    15661 ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca
    15721 ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc
    15781 ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac
    15841 tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg
    15901 atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta tatcgtgtat
    15961 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    16021 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    16081 gtggatttgc cagttctttt gaacgatggt atgttcgaat catcggtggg atagcagtcg
    16141 ttgcagcctt tttaggtatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    16201 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    16261 ttgcctacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    16321 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    16381 gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccag cgaggaaacc
    16441 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    16501 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    16561 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    16621 tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg
    16681 ccgttcatcg atatgatgaa ccagaagtta cggaggaatt ctatgttatg gaagaggcat
    16741 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    16801 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    16861 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    16921 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    16981 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    17041 agatttgtat ttatttatga aggacattta tcagaacaga aaattactgc tacagttttc
    17101 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    17161 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    17221 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    17281 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    17341 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    17401 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    17461 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    17521 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    17581 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    17641 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    17701 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    17761 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    17821 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    17881 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    17941 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    18001 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    18061 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    18121 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    18181 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    18241 atcattgaat ttgctgacat cggcgaccat atttatcaac ctgtaaaaac ctattcaaat
    18301 gggatgttcg ttcgtctagc ttttgctgta gcgatcaatg tgaatcctga tattttgatt
    18361 gtggatgaag cattggcagt aggagatctt gaatttcaac taaaatgtat ggaaaaattt
    18421 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat tagttcgatt
    18481 cgccgttttt gtgatcgttc ctattggttg aaaaatggaa aagttgtcga atatggtgat
    18541 acgatggaag taacgacgaa ttacgaaaac tttttgaaga aaaaatcagt taaaacagta
    18601 gatcgaaata aaaaccaagt agaacgcttc gcagctccag atatcgtaga cgtgacgaaa
    18661 gccgaattgt tggataaaga taaaaatcca gtggatatgc tggaacaagg agaaaaacta
    18721 tacgttaaag taacctatga agtaaaagac gatacaatca aaaatccagt tttaggaatt
    18781 gctctgcgta cagtcgacaa tttttatgtg tgcggactca atactctact agatggtatc
    18841 aagatccctt ggaaaaaagg tgagaatatc ttttatctgg agtatgaaaa gttaggtttg
    18901 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    18961 gtatacaaga caaaatacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    19021 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    19081 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    19141 agttttagag tttggtcctg gaaacggccg attaacgaaa catttgatcg gagcaaaaca
    19201 atgtgaagtg agcattgtcg agctagataa ggaattgttc gattttgtca gcgagtttgc
    19261 ccaagatgga ttttatggag atatcgaaag ttttgaatgg gcaaactatt atgctggaca
    19321 aacatttgat tacgttttgt ttgccgatgt tttagaacat ttggtagatc ctggaaaaac
    19381 gttgaagaaa gttagagaat ttttgaatga agaaggagaa attctaatta cattccctaa
    19441 ccttgcgcat aattctgtca tgattgattt gttcaataat caattaccat gggcttctta
    19501 tggtttgcta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    19561 tgaaaaagca ggattataca tcaatattga agattatttg tacttagctg taggtgatac
    19621 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgttatgatt tcaaaatgcg
    19681 accatttggc gaagtctacc aatacttctt ctcattgatg aaacatccag tagcacaaag
    19741 ttccattgcg cagccgcaaa attctaatta tgtgaaagta ttagaagtaa tccagcaaac
    19801 gaaacaagat gaaataattc aacaaatacc attcaataat ttcacagggg aaaatgagac
    19861 actaagtttc catgtatcag aattagcaga acgaatggtg tttcgcttcg cgcagcaacc
    19921 tagctttatc gaattcagcg cagaagctgc tggagaaaaa ctcacattta tcgattcaaa
    19981 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat ggtaaagatt taccagagtt
    20041 ctttttaacg gatattgcag ggaaagaagt gactatccat tgtcactatc gttttatcgg
    20101 agaattgaca ccaacaatga aagaattgct ggaaacaatc cgtccaatgg ctgaagttac
    20161 aaagcagctt tctgagaaaa atgatgaatt agaaagagaa aatcattatc ttattgagaa
    20221 taatacaaga ttagatcaga cgttgaaaac aaccataaat cgctattgca cgttgttgga
    20281 aagtgatgaa tggacaatca aacatagatt agggcgacgt aagaaagaaa catcgaaaaa
    20341 aatccaagaa aaagagcttt ctctatgtat tgacgaaaaa atctgggatg cagaaacaaa
    20401 gatattgaaa atcatcgggt ggggaatcgc taattcggat cgccaaccat tgtcctataa
    20461 gttatcagcc gaccaatcac cattttttga agcaattcct gttttacgag aagaagtcaa
    20521 tcaagcaaaa cagctagctg aaggagcaaa agcaggattt gaactgcgga tcctttgcga
    20581 gaaggaacgt tcattcttag ttgaagcagt ggctcaaaat ggtcaatcat ggatcattga
    20641 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    20701 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    20761 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    20821 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    20881 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagcaatc
    20941 ttagacttcc aaacagcaag acatcaaaat ggcatagctg tgaaattaaa cggtaaatat
    21001 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    21061 ataaattatg caaggactca tggcgtaaaa aaagcaatcc gccgggtcaa actggaattg
    21121 aaaccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc tgatctgaaa
    21181 gagcaaagga aagaagtaca aggatttgcc taccgcccat tgatttccat acttatgcct
    21241 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact tgatcaaacg
    21301 tatgatcact gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    21361 tgtctagaat cctatcaagc aaaagatgat agaattaaag tcgttttccg acaagaaaat
    21421 ggacacatca gtctggcaac taattctgca ttgaaaatgg cagaaggaga gtttatagcg
    21481 ctcttagata atgatgacga attacctcca tttgccttat atgaagtggc aaaagtgcta
    21541 aacgtacatc cagaactgga tttgatctac agtgacgaag ataaaatcga tgcagatggg
    21601 aatcgctttg acccgcactt caaagcagac tggtctcccg atacattgat gggaaataat
    21661 tatatctctc acttaggcgt atatcggaca agcattgtta aggaacttgg cggtttccgt
    21721 aaaggatatg aaggctctca agattatgat ttggttcttc gtgtgacaga acaaattcct
    21781 gcagaacaca tttaccacat tgaccgtgtc ttgtatcatt ggcggacgat cccgggatca
    21841 actgcaagca atggagaagc taaaagctac atctatgatt ctggagtgaa agcattgaca
    21901 gatgctctat ctagaagaaa tatcaaggga agcgtacatc ctggcagaat ttctggtttt
    21961 tatgaaatca cttatgatgt tttgcaagaa gacttggtca gtgtgatcat tccaactaag
    22021 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttatcca
    22081 aattatgaaa tcattattgc agataatgga agtacagatc ctaaaatgca agaattattt
    22141 gcagaataca agcatcagct gaaagaccga tttatcgtcg aattaattga cattccattc
    22201 aattattcac gtatcaacaa tctggcagca gaaaaagcaa acggcaaata ctttttattc
    22261 ttgaataacg atacagaagt gattgaacct gactggatga cagcgatggt ttcttatgca
    22321 cagtttgatc gaatcggctg tgtaggagca aaattatttt atccggatga tacaacgcag
    22381 catgcaggtg tattattagg gatcggtggc gttgcaggac atgcattgaa taactatgat
    22441 cgaacacatt gtggttactt tggccgattg gtcattgacg tcaattactt agcagtcact
    22501 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg tcaatggatt tgatgaaata
    22561 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    22621 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    22681 gatacaccag aaaaacaaag acgttttgct ggggaaatca aaaaaatgca agataagtgg
    22741 ccttcttata ttgctcatga cccattttat aacgataatc taactaaaga aggaatcggc
    22801 gatttttcgt tgagacctga ttgattaaaa gaactgtatt ttcattttta ttaatgaaaa
    22861 tacagttctt ttaatttaat tatatgattt gtcaaattaa gctagaataa aatgcttctt
    22921 gtacatagct caaaaatatt tcgattggtg ttctgtaatt caatgatttt cttggaatat
    22981 gattacgttg attgctgaca ctggaaataa atgtctgatt cacttttcta aaatccattg
    23041 atttcggcag tccattacga cgcagaatcc cgttagaatt ctcgtttaat gggcgttgag
    23101 aaggtgttcc agggtccgca aaatatatat caatatcatg ttggttacta atggctttcc
    23161 agttagaaaa ttct
//
LOCUS       DACDNO010000048        22787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDNO010000048 DACDNO010000000
VERSION     DACDNO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.048"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(41..1420)
                     /gene="brnQ"
                     /locus_tag="IWI22_001909"
     CDS             complement(41..1420)
                     /gene="brnQ"
                     /locus_tag="IWI22_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9169298.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            2126..3097
                     /locus_tag="IWI22_001910"
     CDS             2126..3097
                     /locus_tag="IWI22_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9169299.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            3121..5313
                     /locus_tag="IWI22_001911"
     CDS             3121..5313
                     /locus_tag="IWI22_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9169300.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            5330..5806
                     /gene="ybeY"
                     /locus_tag="IWI22_001912"
     CDS             5330..5806
                     /gene="ybeY"
                     /locus_tag="IWI22_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9169301.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            5784..6185
                     /locus_tag="IWI22_001913"
     CDS             5784..6185
                     /locus_tag="IWI22_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9169302.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            6200..7099
                     /locus_tag="IWI22_001914"
     CDS             6200..7099
                     /locus_tag="IWI22_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9169303.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            7242..8054
                     /gene="recO"
                     /locus_tag="IWI22_001915"
     CDS             7242..8054
                     /gene="recO"
                     /locus_tag="IWI22_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9169304.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            8438..9355
                     /gene="glyQ"
                     /locus_tag="IWI22_001916"
     CDS             8438..9355
                     /gene="glyQ"
                     /locus_tag="IWI22_001916"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9169305.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            9357..11432
                     /locus_tag="IWI22_001917"
     CDS             9357..11432
                     /locus_tag="IWI22_001917"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9169306.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKITNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLNQIAAMALSL
                     ASLDQIITK"
     gene            11822..12601
                     /locus_tag="IWI22_001918"
     CDS             11822..12601
                     /locus_tag="IWI22_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169307.1"
                     /translation="MEKPFVKSLTAVAVGAGAIVICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVIQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNISY"
     gene            12647..13591
                     /locus_tag="IWI22_001919"
     CDS             12647..13591
                     /locus_tag="IWI22_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9169308.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGSDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            13607..14386
                     /locus_tag="IWI22_001920"
     CDS             13607..14386
                     /locus_tag="IWI22_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9169309.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKRTAPARPKATNDKTAPSRRSRSRV"
     gene            14398..15096
                     /locus_tag="IWI22_001921"
     CDS             14398..15096
                     /locus_tag="IWI22_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9169310.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            15124..15888
                     /locus_tag="IWI22_001922"
     CDS             15124..15888
                     /locus_tag="IWI22_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9169311.1"
                     /translation="MIDLHCHILPGIDDGSENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            15902..17721
                     /locus_tag="IWI22_001923"
                     /pseudo
     CDS             15902..17721
                     /locus_tag="IWI22_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378780.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
     gene            17732..18364
                     /locus_tag="IWI22_001924"
     CDS             17732..18364
                     /locus_tag="IWI22_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9169312.1"
                     /translation="MKAIIIGAGGFGKEIAFLLQSVSRYELIGFVDDSLKMKNQELLG
                     KPVLGTIDSLIELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVAPSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDAQIGNYNSIFPSVNISGHVILGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNNTKNVGVPTKVIERWD"
     gene            18366..19484
                     /locus_tag="IWI22_001925"
     CDS             18366..19484
                     /locus_tag="IWI22_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP9169313.1"
                     /translation="MENKRILLASPHMSEEGYEQEYVKEAFDTNWVAPLGANVNGFEK
                     ELAKYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKNELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINTNHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            19477..20094
                     /locus_tag="IWI22_001926"
     CDS             19477..20094
                     /locus_tag="IWI22_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9169314.1"
                     /translation="MRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLGS
                     PIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELPE
                     LWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKFD
                     LDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNTPEKFKGS"
     gene            20221..21390
                     /locus_tag="IWI22_001927"
     CDS             20221..21390
                     /locus_tag="IWI22_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9169315.1"
                     /translation="MCRVLNILFQYENIIKKYKEKRVMAKYYNKKILIMANNDDGLYR
                     FRGMLIKNLVDQGNEVIVSLPKGKNISKLIELGCQIIETPIDRRGINPKTDFKLFFNY
                     CNILKKIKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFL
                     YRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVH
                     FLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYH
                     GYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGY
                     LCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            21436..22260
                     /locus_tag="IWI22_001928"
     CDS             21436..22260
                     /locus_tag="IWI22_001928"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9169316.1"
                     /translation="MSNVSNPLVSVIMGAYNSEKTIGRAIDSIIKQTYLHWEMIICDD
                     GSTDGTVKIIRKYVEKDKRIKLVQNKKNSGLAYSLNHCLKYSSGKYVARMDADDISLP
                     ERFAYQVGFFEKNPEYDVVGCNRIVFDENGDSGIRKSKEIPTINDMYKGSPFAHPTIM
                     MKRSVYTILGGYRVNKDTTRSEDLEMWFRFFNEGFRGYNLQRVLFKYQETSEDFHKRS
                     LRAAYGITKVLLRGYKLIGVSKYKYIYAFKPIISAIIPKYIMYRYQERRLHNTEVL"
BASE COUNT         7975 a         3812 c         4642 g         6358 t
ORIGIN      
        1 cctgtttaat atctcttatg ttaaattgtg tttgatttgg ttattcttct ggtagttcat
       61 ttgcttcttg tttataccct gataggcgat aggcgtaata aaagagtaag ctgccagcgt
      121 aaccaagtaa cgttgggacc atccagccaa gtcctaatgc agcaaaagga acgtgatgat
      181 gataaaatcc tagtaagctt gctacgacag tttgctgact cactaatgca ggcgaatttg
      241 ccaacatatc caatacggct ggaacggcag aaaagaacat cgttacttga tagacaaatg
      301 gactcttttg gaagaatttt gctgttaagg aaagcaagat taacgcaagt gacaatggat
      361 acaaaagcat caatactggt aaagaccatt ggatgatatt atccaatccg gcattcgcaa
      421 cgacaaaaga gacaaaagtc gttaaccgca gccatgtcat ataactgact ttagggaaca
      481 gtttatggaa atcttgtgca aaagaaacaa cgagtcccat agcggtagtg aagacaccta
      541 atgtgaccat tacccctaag aagctagcac cgaagttacc taaataaaaa ttagtgatct
      601 gagaaagtgc agttccgcca ttttctgata aaggcatctt attcaaactc atcgcaccga
      661 gaaggaccag accaaaataa atcatgactt ctaacgcaac gctcaggcta ccagcttttg
      721 ctgttaattc tgtgactttt ttatctttaa aaccaagatc acgaactgca tggacaaaag
      781 tgacacttaa agcaagtaaa gctactgcat caaccgtgtt gtatccttct aaaacgccat
      841 ttgtcaaagc gttcgtctga taagaatctg cagcagattg gtttagattc cccatgggtt
      901 ggaataaaga aacgacaaaa atgatagcta ataatgcaag aaacaaggag tttaaatact
      961 ttccgacata tttgaccaaa cgtgttggat gtacagtaag gaaataagca agtaaaaaga
     1021 aaagagcaga gaataaaaac atacctaatg aatcatattt ttcaggcaaa aagggtttag
     1081 cagccatttc aaaagcagtt gctgctgtcc gcggtgtacc aaagaatggt ccgatcgtta
     1141 aatgaacaag gatcaagaac aaggcagcat accattttcc gacaggttta gctaagtcat
     1201 acagaccatc actttttgtc actactacag ctaagatagc taataatggg aaaagagcgc
     1261 ctgagatggc aaatcctaca ccagcgctga tccagtttga tccagccatt tgacctaagt
     1321 ggacaggaaa gatcagatta cctgacccga agaacatccc gaagataagt gaactgacca
     1381 tcaataattg cttccatgaa taagtttttg tttcttccat aaatttttcc tccaataatt
     1441 atcattttga gtaacaaaat aaacgttgca gaaactagaa atgccactaa tatcagattc
     1501 catgaaaacc accaccttaa aatataaaaa aaagcccttt tccacttggg aaaagggctc
     1561 tagattctag aacggtacca ccttttttcg cttaaaaaag cttcttcatt cacggacgct
     1621 tttcgataaa gttatccgct agcctgataa tggaggcagc cacaatgatc tacgttttcg
     1681 acattgactc ggaaatgatg ttcgtctatt cgatttattg tcatttctca cttacatgac
     1741 tcactgggaa taaatcgctg aatagcctac tgttttcctc gacgctttgc tgtatattta
     1801 tttgttgacg ttataataca cctgtttttt ttgaaatgca agaatatttc aaatattttg
     1861 ttttcttttt ttgaaacaag aaaaataccc agagaatact tgctaagtta gacggaatac
     1921 ttgtcctcta tcttatgcat agcttacatg atcagctttt tttattcaaa cgagaccttt
     1981 agagaatgga agcagctatt ttttaacgtt tcttggtact tatggttaag caactctttt
     2041 agaacctttt cttatttgct atatagtttc ttctgagtat ggtaaaatag gaaagaatga
     2101 atcgaaaaaa aggggatgtc tatttttgac ggaagagtct agttctttag agattagatt
     2161 gacggctgct gatgatgtca gcatgctttt aggtgcacac gacaagcata taaaactgat
     2221 tgaagaaaca acagaaacaa ccatccatac acgtggagaa atcattcaga tcattggcga
     2281 acaatcccag atttccttgg ctgcctcagt cattcggaca ttgcaggaat tgatcaaacg
     2341 aggaatccat gtaagtacac cagatgtcgt gacggcactg aaaatggggc aaaaaggcac
     2401 attagattat tttgttgaaa tgtatgagga agagatcgtc aaagaccgta atggaaaacc
     2461 aatacgggta aaaaacagcg gccaaaagaa atatgtcgaa tcggtagcca aacatgatat
     2521 cgtatttggc gtaggaccag ccgggacagg aaaaactttt cttgcagtag tattagcaat
     2581 cgctgccttg aaaaaaggtg aagtgcaaaa aatcattttg acgcggcctg ctgtggaagc
     2641 aggggaaaat ctaggctttc ttcccgggga tctaaaagaa aaagtagatc cttatctgcg
     2701 tcctgtttat gatgctttat accaaatttt tggattagat catacgaacc gattgatgga
     2761 acgaggcgtg atcgaaattg ctcctttagc ttacatgcgg gggcggacat tggatgatgc
     2821 attcgttatt ttggatgaag cacagaacac gacagttgct cagatgaaaa tgttcctgac
     2881 acgtcttggt tatcaatcaa aaatgattgt caatggagat acgagtcaga ttgatctgcc
     2941 tcgagggaca acaagtggtc ttgttcatgc agaacgaaca ttaaagcaaa tcaagaaaat
     3001 cgattttgtt aattttgaag caagtgacgt tgttcggcac cctgtagttg ccgaaatcat
     3061 caaagcttat gaaaaggcag atctccatca agaataagct ttgcttagga ggataagcac
     3121 atgttgaata aaacactgca gaaactacaa gaaaaacttg gaaaggcagc attgcctttc
     3181 ctgctgttct tgttttttat cattatctgc ggcgttacat ttagtagtgt caaacaaaag
     3241 gccaatgatt tcagagaagg ccaagtagca gaagaaagta tccgtgcgaa caaaacgatc
     3301 gagaacacgg aagaaaccga acaaaaaaga aaactggcag cagaagcagt cactccagaa
     3361 tacacttacc aaaaagatct tgcagatgac cagaacaatc ggatcaagca actatttgaa
     3421 ttgatcgata aaacgaatga tgccatcaac aaatcttatg acgaaaagat tgataaggca
     3481 aaggaagatg aaaatatccc aaagcctact acagaagagc ggatcgccgc gttgaagaaa
     3541 aattttgaaa atgtgaatgc agaaaatgtt gcgttttatc aaaaactacc gaacaatttt
     3601 tattcgacga tatttgaaat gactgaaact gagatccata ctgtgaggga tgagagtctg
     3661 aagctcattg acgaacagat gagcaaacaa gttcgtgaaa gtgaattgga agcttttaaa
     3721 caagaagcag aagagcaaat ccaatatttg aatgtcacac cagcacaaca acaaatgatt
     3781 cgctacttag tggatcaagg tatcgtagtg aatgacgtat tgaatgagaa aaagacagaa
     3841 gaattgaaac aatccgcacg agaagctgta caaccagtga tgatcttcca aggagaaatc
     3901 attgttcgtg aaggtaatca aatcgatgct tcagctatga aaaaattgga attactagga
     3961 ctgactaatc aaacaacttc gatttttccg ctaattgcca tggttttagc tgttttgctt
     4021 caaatcggcg tactgattta taattcactt caattccaaa atgcaggaga aagaacaaaa
     4081 tacgtgcttt tctatgtgac agctatgtca gtcagcatag tgctgatgaa atttttccaa
     4141 ttattccaaa cggaacaagc tacttatatc ccgctgtttt atcccgctgc ttttgcacca
     4201 cttgttttaa gctttttctt gaaccgaaga gcagggatca tggctggtat tttccaagca
     4261 gtatctgcac ttttcatttt ttatgattcg attggcacga actttctaac gattatcttg
     4321 atgtcttatg ttttctctgg gctcatggca acgattgtca gacgcaaacg gatctcagaa
     4381 caaggattat ctgctgccat gtgggtcatt atttttcctg tgatgatgga tgtcattttg
     4441 atcgtctatc aaggaatgag ctttggtgat gcaacgactt ggttaatgct tgtctgcggg
     4501 ttcactggtg cactcttctc attcttgctg acgatcggtc tacacccata tattgaattg
     4561 atggtcaatg atgacagtgt aattgtatta aatgaactca gcaatccaaa tcatccttta
     4621 ttgaaacagt tgctggaaga agcgccaggt acttatcatc atagtatgat ggtagctaat
     4681 ctaagtgcta acgctgtagc agaaatcggc ggacgttctt tgctaactcg agtagcttgc
     4741 tattatcatg atatcggcaa gatcaaacat gctagctttt tcgttgagaa cttgccttca
     4801 ggagcggaaa acccgcacaa tttcttattg ccagaagaca gtaaacatat tatctttgga
     4861 catgtgacag atggagccaa aatattggaa gattataata tgccgcaaat ggtgatcgat
     4921 atttgcaaac agcatcatgg aaccacgcta atgcgttatt tctacatcaa agcgaaagaa
     4981 cgcaatccgg aagtaacaga agagcaattc cgttatcctg gaccaaaacc gcaaagtcgt
     5041 gaagcaggga tcgtcaatat tgctgacagt tgtgaggctg cagtacgggc aatggatcat
     5101 ccgacgagcg aaaaaatcga aagttttgtc cataatctta tcgaagagcg tatttccgat
     5161 ggtcaattag acgatagtgg actgaccttg aaagaaatcc gaaaagtgga aaaatcattg
     5221 atcagcggtt taagtagcac cttccattct cggatcaaat atcctaaaat gaaaacagaa
     5281 gttgagaaga taaaggaaga acaagaagaa tagaaagaag cgatgtataa tggatattac
     5341 ctttatggat gaaacaaacc aagtctcaga agagaaaatg aaagaaatcg atgacttgct
     5401 gcaatttgca gcatcttatt taaagctgcc agaggaaaca gaaatgtctg ttactttcat
     5461 ggacaatgcg gcaatccaag taatcaatcg tgattaccgt ggaaaagatg caccgacaga
     5521 cgtcatcagt tttgctctgg aagaagaagg cgaagacgaa atcccagtga tttttgaaga
     5581 tggagagaat ccattgcctc gtgtactagg agacatcatg atttctattg aacgtgctgc
     5641 tgaacaagca gaagcttacg gccattcata tgatcgggaa cttggctttt tagcggtaca
     5701 tggattcttg catatcaacg ggtatgacca tatgacacca gaagatgaga aagaaatgtt
     5761 tggtttacag aaagagatct tggatgccta tggactcaaa agataaaaac gtagagaaaa
     5821 acaaacattt cctgatgtct gtggaatttg ctgtccaagg agtcaaaacc gtttttgagg
     5881 aagaacgcaa tatgaggaaa catgttgcgt tcggtatcct tgctttattt atgggcgtga
     5941 ttttccagct tgatcggata gagtggttat ggctgctttt agctgttttt ctcgtttgga
     6001 tagtagagat catcaatact gtttttgaaa atgtagtgga catgtttacg gattttcatt
     6061 ttcatccgat tgggaaaaaa atcaaagata tggcagctgg agcagtttta gtgaccgctt
     6121 gttttgctgt catcattggg ttgattttat ttgtccctaa aatatatcat ttgttgttta
     6181 attaaaaagg agaacgtata tgccagaaca taaatcaggg tttgtcgcaa tcgtcggccg
     6241 cccgaatgta ggaaaatcca cattattgaa ccgaatcgtg ggtcaaaaaa ttgccattat
     6301 gagtgacaag gcacagacaa ctagaaataa aatccaagga gtatatacta caccagaagc
     6361 tcagctgatt tttatcgata caccaggaat ccataaacca aagcatcgtc taggagattt
     6421 tatggtagaa gcagcttaca gtgcgttgaa agaagtggat gcagtcttat ttatgatcag
     6481 tgctgatcaa aaacgcggcc ggggagacga ttttattatt gaacgtctaa aaaacgtgca
     6541 ttctccagtt tatctgatta tcaataaaat cgataagatc catccagacg agttgctggg
     6601 aatcatcgaa gattattcga cgcagatgcc ttttgcacaa gtgattccga tctctgctac
     6661 agaaggaaac aatgtcgaac gcttaatgga tgtattagtc agtgaaatgc cagagggccc
     6721 acaatatttt ccagatgatc aagtgactga tcatcctgaa tactttatcg tttctgaatt
     6781 gatccgtgag aaagtcttgt ttttaacacg agacgaagtg cctcattctg tcgcagtcgt
     6841 tgttgattcg atgaagcgaa acgagaatga taaaatccat atccaggcca cgatcatcgt
     6901 cgaaagagat agccaaaaag gaatcattat cggcaaaggc ggaaaaatgc tgaagcaagt
     6961 aggaacgaaa gcaagaaagg atatcgaaaa tcttttagga gacaaagtat ttctagaact
     7021 ttgggtcaaa gtccaaaaag actggcgaga caaaaaagtc tatctgcaag actttggcta
     7081 ccgaaaagac gaatactgat attctgaact tcttataggt taagtataga aataaactgg
     7141 ataaaaatcc gcacgttgag aaaaacggcg gaagatcgaa acataaataa tctggatcgg
     7201 cagaatctgc ttttccagat ttatttttta ggagggagaa tatgagccag cttgcagaat
     7261 cgaaaggctt gattttatct agccgtaatt acaaagaaaa agataaatta gtcaaaatct
     7321 ttacggaatc ttctggcaaa atgatgtttt atatcaaagg aatccatcgg caaaatcagc
     7381 cattagctcc ggctttgctc ccctttaccg aagcagtcta tatcggtaat tttcgaagtg
     7441 aaggtttgtc gtttttaagc agtgtcaaag acgtccatgc gtttcggaac atccaagaag
     7501 atatcttttt gagcggttat ggtacctatt tgctaaatct agtagatgca gcaatcgaag
     7561 atcatcaata tgacccgcat ttattccaat ttacgcaaca agctctgcag cggatggatc
     7621 aaggagatga tgcagaaatc atcacgaata tctttgaagt tcaaatactg caacgcttcg
     7681 gaatcgcgcc aatttggacg cactgtgtcg tatgcgggga aacaaaagga aaatttgact
     7741 attcttccaa atatggcggt gtgatatgcg agaaacactg gccaatggac gaacaccgtt
     7801 atcatgcaga tcctcgcgct gtttatttcg ttcgaatgtt ttcggctatt tcttatgata
     7861 agatcagtgg aatccacttg aaagaagaaa cgaagcaggc aatccgtgac ctcattgacc
     7921 agttgtatga tgaatatgtc ggtattcatc taaaaagcaa aaagttcatt gatcaaatga
     7981 agtcttgggc acacgtatta aagacgccag agaaaaatat gcctgaaaaa gagatacctg
     8041 aaaaagaaga ataaatgaaa gatacaacaa aaaaatcaaa actatttgac aaatacaggg
     8101 acttccgtct attattaacc gtagattaaa tagatcgttc actgagagga tctattatat
     8161 aaacaagaaa actgcaaaat gaatacgtac attgaccaag atcagtaaaa aacacgtggt
     8221 ttagcgagtt cgggagagtg gaagccggac ccaaacgatt tttgaagagg cacttgcgag
     8281 tacgataaat gaagctgccg aatctttatt cggaagtagg gtggaaccgc gaaattattc
     8341 gtccctatgt ctgtaatcag acatagggac tttttgtcgt tttctttcct gtattcttat
     8401 tgacagcaac gcttaaaaat tgaaaggggc aaaacagatg agtaaaaaat tgaccgtcca
     8461 agatatgatt ttaacattac aaaaattttg gtcatcaaac ggctgcatgc tgatgcaagc
     8521 ttatgataca gaaaaaggag caggtacgat gagcccatat acattcttac gggcaatcgg
     8581 accggaacca tggaatgctg cttatgtaga gccatcccgc cgaccagctg acgggcgtta
     8641 tggcgaaaac ccaaaccgtt tgtatcagca tcatcaattt caagtcgtaa tgaaaccttc
     8701 acctgaaaat atccaagagc tttatttaga aagcttgcgt cttttaggaa ttgatccatt
     8761 agaacacgat attcgttttg ttgaagacaa ctgggagaac ccatcgatgg gttgtgccgg
     8821 tgtgggctgg gaagtatggc ttgatgggat ggagatcaca caattcacgt acttccagca
     8881 agtcggagga ctaccttgta aaccagttac ttctgagatt acttatggac tagaacgtct
     8941 tgcttcatat attcaagaag ttgaaagtgt ctatgatctg gaatgggctg atggtgtgaa
     9001 atatggcgag atcttcaaac aaccagaata tgaacattcc aaatattctt ttgaagtaag
     9061 cgaccaagac ttgctactaa gcaattttga ccgttttgaa aaagaagcta aacgctgtat
     9121 cgatgaaaat cttgttcatc cagcgtatga ttatattttg aaatgcagcc atacattcaa
     9181 cttattagat gctcgcggtg cagtatccgt aacagagcgt gcaggttatc tttcccgtat
     9241 ccgcaacatg gctcgtgctg tagccaaaat ctttgtggca gaacgcgaaa aattaggatt
     9301 ccctttattg aataaaaaca cagcaaatcc agaaaacatg aaggaggctg aataagatgg
     9361 caaaagactt attacttgaa atcgggctgg aagaaatgcc tgctcatgtt gtcacaccaa
     9421 gcatgaaaca actagaagaa aaaacagcaa aatttttaga tgaaaaccaa ttgacttatg
     9481 atacgatcga aacgttttct acaccgcgcc gcttagcaat caaaataaca aacattccag
     9541 aacgtcaagc agacagtgaa gaagaagtca aaggaccagc caaaaaaatt gctttggacg
     9601 cagatggaaa ctggacgaaa gcagctgaag gatttgtccg cggacaaggt ctgacgacag
     9661 atgctatcac ttttagagaa ttaaaaggcg tggaatatgt ctatgtaacg aaatacactc
     9721 acggcaaaga agcaaaagaa gtattgacag gtttagcaga tgtcattaaa agcctcactt
     9781 tcccagtaac gatgcattgg gcagaatacg attttgaata tatccgccct attcactgga
     9841 tcgtggcatt gttagatgac gaagtaatcc cgtttgagat cttggatatc aaaacagaca
     9901 gagcttcaag aggtcatcgt ttcttaggtg aagatgtgac atttacgcat gcaaaagaat
     9961 acgaagaaaa attaaatgaa caatttgtta ttgcttcacc gcaaaaaaga aaagaaatga
    10021 tcatcacaca agcaaacgaa ttagcagaaa aaaatcactg gatcattgat ctagatgaag
    10081 atttactaga agaagtcaac aatctagtcg aatatccaac tgtcttttct ggcgacttcg
    10141 aagaaaaata tctatctgtt cctgaagaag tactagtcac ttctatgaaa gaacatcaac
    10201 gttatttcga agtacggaac gatcaaggta tgttgcttcc tcattttatt tctgctcgta
    10261 atggcgatcg ggtaaaattg gaaaatgtgg tacgaggcaa tcaaaaagta ttgactgctc
    10321 gtttagaaga tgcggaattc ttttataatg aagacaagaa agcttcaatc gaccaatacg
    10381 ttgaaaaatt aaaaactgtt actttccatg aaaaaatcgg tagcatctac gagaaaatgc
    10441 aacgggtaca agtcatcgct cgcatcatag gcgaacaagt aggactatct gaggaagaat
    10501 tgatggatct gcaacgcgct gctgaaatct ataaatttga tctagtaacc aacatggtag
    10561 gagaattccc tgaactacaa gggatcatgg gagaaaaata tgctctgctt aaaggcgaga
    10621 aaccagccgt agcaacagcg atccgtgaac attacatgcc tatatccagt gaaggagatc
    10681 tacctgagac tacagtaggt tctgttttag cgattgctga taaattagat agtatctttt
    10741 ctttctttgc agttgggatg atcccaagtg gctcaaacga tccatatgct ttgcgccgtc
    10801 aagcatatgg tgttgttcga atcatcgaat cgaaaaaatg ggcctttcct ttgtctgttc
    10861 tgcaagaaac gattagtgaa gttatcagta aagatacgga tcgttttggc attggcttgt
    10921 cagcgggaca acaacaggtg atcgacttta tcaaaggccg tctgcgccaa ttattgacaa
    10981 cgaagaatat tcgtcatgat gtcatcgaag ctgttttgaa cgcagaacaa aaagatctga
    11041 caaaagtatt tgcagcagca caattgttca agcagcatct tgcagatgaa gacttcaaac
    11101 catcgatgga agctttgaca cgtgtcgtga acttagcgaa aaaagcggag cttgaaaaac
    11161 aatcagaagt agaccctgaa ctgttcgaaa acgaagcgga gaaagaactg cacaaagcag
    11221 tagaggcaat gaaaacaagc tttgctgacc aaacgatcaa cgaaaattat gatcagcttg
    11281 taagccttcg cccactgatc gaaaattatt ttgatcagac gatggtcatg gcagaagaac
    11341 cagcagtacg taagaaccga ttaacgcaat taaatcaaat tgctgccatg gctttgtcac
    11401 tagcaagttt agatcaaatc atcacaaaat aatttctaat atgtaaataa accagagaac
    11461 aggcacaaaa taggctattt ctctggttta tttgttataa atataaataa ctattattaa
    11521 ttcaatgtta atttttttgt tattttattt tttgtgtaac ttttaaaaac aactatgaaa
    11581 attaaggaaa taaaaatgaa atcgattaat tgttatctta ttaatgtttt atttactttt
    11641 aaatcgtaaa ttgccctagt caagaaaaca gtttgtggta tgattatata gagaaaactt
    11701 gtttataaat atttctgtga atatattagt ttaattatgt tttttaaaat aaaaagctgt
    11761 gatttttatg taagtgaatt atatcttaat ttatctatca tcaatactta ggagtgaatc
    11821 tatggaaaag ccattcgtca agtcattgac tgctgtggcg gtaggtgcag gtgctattgt
    11881 gatctgtttg tttggttacc atatcaataa tcagcggcaa catcagcaac ggatcaatta
    11941 tgcagaatct gccatcacca atcaaaaaga caccttgact tctttatcga aagaagtaga
    12001 caagctgtat agtacaaaag aaaaaatttt tctaaaccct gaaataacag aagaaacaat
    12061 tacaagtttg tccaataagc tatcctctgt caaactttca gcagatgatt acgacatcaa
    12121 agaatcagaa ttgcctaaag aagctgttgc aatccaagat gagaagaaag caatcgtgac
    12181 acagttagat agtgcagaat caaaactgaa gatccagaca gctgtcaacg atttatttac
    12241 taaaaatgta tcaaactggc agcaagctgt agacgatgtc atcatcaaag aaaaggtgac
    12301 aagtgcagat gtcgctcgtg tacgtgaaaa tatgagcttt tttaaagata gtccttggaa
    12361 aacggttgtt atacaatatc taggattcgc tgatacccag atcgcgcaag tcacgcaact
    12421 agatcagtta ttcgatacga tgttaaagga tggtcaagtg actgccaccg ctacttacga
    12481 tcaatatttg acggcgctca gtcaaataga gcagatccgg aatgaaaaaa taagcgtagc
    12541 atacgcaaca aaagcacaaa ctgttgctca gcaaatgggc tatagcaata taagctattg
    12601 acagttaaag cgttgattca agcaaggtta gcaaacggag gaaactatgg caagacgaag
    12661 aagaaaatca agagggaaaa aaatatttct gattatttta gcagttctta tcgtattagt
    12721 attagcaatt gtaggagtag gagcaaaatt atatctggat ctctcacatt ctattgagaa
    12781 aacttacgaa tcagtggaac gaacccaagg agaacgaacg aaaccagttg atctggacaa
    12841 acaagaatct ttctctgttc tattgatggg gattgatact ggagatttag gtcgggtcga
    12901 tcaaggaaga tcagatacga tgatggttgc cacagtcagt cctgaagaca aacagacaac
    12961 gatcgtcagc attcctcgag atacgtatgt cgacatcgta ggtagtgaca caaaagacaa
    13021 gatcaaccac gcttacgcat tcggcggagc tgccatggcg atggataccg tacaaaaata
    13081 tgtagacatc ccaatcgatc actatgtctc tatcaatatg aaagggctaa aagaacttgt
    13141 tgatgcagtt ggtggtatcg aagtcaataa tgacctcact ttctcacaag atggctatga
    13201 ctttacaatc ggtaaaatta ctttgaacgg ggatcaggca ttatcttatt ctcgtatgcg
    13261 ttatgaagat ccaaacggtg actatggtag acaagagcgt caaagaaaga tcgtagaagg
    13321 tgtagcgaaa aaagtattga gtctaaacgg tgtcagcaac tatcaatccg ttttgaatgc
    13381 tctagagtcg aatatgaaaa cagacatgag ctttgatgaa atgaaaaaaa ttgctttaga
    13441 ttatcgggag gcatttggta cgatcaagca agatcagctg caaggagaag gcttcatgca
    13501 agataatatc tcctatcaaa aagtcagtga tcaagaactt gatcgtgtga aaaaagaatt
    13561 aaaagatcaa atgaatctag agaataaata ggaaagtgga gaaaaaatgg aagagacaat
    13621 cagcttgaaa gaaatttttg aaacattacg caaacacttg gtaacaatca tcatctcaat
    13681 gtttgctggc ttggccatct caggaatcgt aacatttttc atcttgacac caaaatacag
    13741 ttcacaagca caattgatcg tgacactgcc acaatcagac acagcaaatg tcaatgatgt
    13801 caatacgaac ctgcaaatga tcaatacgta taaagatatg atcgtcagtg atttagtact
    13861 aaatgaagtc aaagatcgtt tagaaacaga agacaatgtg aaaatgactg ctggacaaat
    13921 caaagatgcg atctccgtca atcagtcaca aaattcacaa atgttctcga tccaggcaat
    13981 aagcacgaat gcagtgactg ctcaacagat cgctaataca acttcacaag ttttccaaga
    14041 aaacgcaaaa gacgtgatga atgtcgataa aatctcgatt atttcaggtg cagttgccaa
    14101 tacgacacct gtttcgccaa ataataaatt gaatctagtg attggcttag tattaggaat
    14161 gatggtaggc gtaggtttgg cattcttatt cgaattactt gatcggacag tgaaagatga
    14221 aaaatatgtg acggatacat taggattccc aatcttagga acagttccag aaatgtcagc
    14281 gaaagaattg aacgctggta tccgtaaaaa aagaacagct cctgcacgac caaaagctac
    14341 aaacgataaa acagccccat cacgcagaag ccgctcaaga gtctaggagg aagaaaaatg
    14401 gcacgaacac agaaacaaaa agcaacacaa actcatgcag tcagtttgat cacattaaca
    14461 aacccatctt caccaatcgc cgaacaatat cgtacgatcc ggacgaatat ccagtttgcc
    14521 tccgcagctg gtcagcaaat caaaacgatt gtcgtgactt catcaggtcc tggagaaggg
    14581 aaatccacca ctgcagcaaa cattgcagtc gtttttgcta aatccggtca acgagttcta
    14641 ctagtcgatg cagatttacg taaacccgtc gtctataaga cattccagtt gaacaatgcc
    14701 agtggattaa gtacagcact tagttcatca ggaagtgtag cagatgtgat ccaacgaacg
    14761 ccagtcgaga acttgtcgat tttgcctagt ggacctaagc cacccaaccc atcagaattg
    14821 ttaagctcgc caagaatgga tcagatccta gctgaagcaa gacagttatt tgacgtggtg
    14881 atattcgata tgccaccagt agtagccgta acagatgcac agatcatgtc ttcaaaaaca
    14941 gatggcactt tattggtcgt acgtgaaaat atgtcacgta aagaatcctt gactaaagca
    15001 aaagaactgc tagagatggt acaagcacga gtcttaggtg tcgtatacaa cggagcagaa
    15061 cacagcaaag atgctggtta ttactacgga aattaaggaa taagaagaga ggagacaaga
    15121 gaaatgatcg atctacattg ccatatccta ccaggaatcg atgacgggtc agaaaactta
    15181 gaagcaagta ttgcaatggc tgaaaaagcg attcagcaag gcattacgca tattttatgc
    15241 acaccacacc acaataatgg aaaatacagc aatgaaaaat cacaagtcat ttctctagtc
    15301 gcttcgttac aagccgaatt agagaaaaga cagttgcctt tgacgttatt ggaaggacaa
    15361 gaagtacgta ttaccggaac gctcatcgaa gacatccacc gagatgaaat tttatttaca
    15421 gatttagatg atacatatct tctgatcgaa tttccgacac tggaagtgcc tttatatgca
    15481 gaacggcttt tttttgagtt gttagaacta ggaaagacac cagtgatcgt ccatccagaa
    15541 cgcaacgcgc attttcgaga agacccaaat catttgcttc cgttcctaga tatgggatgt
    15601 ttggcccaac ttactgcgcc aagttatgtt ggtgtatttg gcaaagacat ccagaaaaca
    15661 gccaaaacaa tggtcaaaca taatctcgtg cagatggctg cttctgatgc acacggggtc
    15721 tctaagcgca ccttttactt aaaagagtgc tatgagcaga ttgccaagga gtttggtaag
    15781 gaaaaagtag tacagatgaa acaagtcgca aaggatctga tcaacgggga tgagatcaat
    15841 tatccaatct atgaagaggt aaaaaagaag aagtttggtt tattttaggg ggagttaaaa
    15901 agtgtttata ttaacaagaa gaagaaaaat tgccacattg gttgttgtgg acagcttgtt
    15961 attaatcgct gcaaatattg cagcagctaa attcatgaag ccgtttgtat ctataccaat
    16021 ggatcttatt ttaatctcta tcggtttgtc gattggtttt tatttatttt atggtagttt
    16081 attcaaagtt ttcactcgga tcaatcgcta tacaaattta agagagatca ttgcaatttt
    16141 tagttcacta tctgcaagtg ctgcctcaag tatcttcata ctgctgttta ttaatcggcg
    16201 atacagttta cggttagtta tctttactta tctgctttcg ctcttattga tcatcggtag
    16261 ccgattgatc tggcgtatct acatcgaaaa aaaaaaaata tgagatatgt atcagcagat
    16321 tctgctaaaa atacattgat tgttggtgct ggcgaaggcg ggcgtatttt atacaacagc
    16381 tttcttggat ccaaaacggc tcaagatatc cacgttgtcg ggtttgtaga tgatgatcca
    16441 aataaaagaa atacgtatct ttcaggaaaa aaagtattag gtgcattaaa agatattccg
    16501 gaattgattg aaaaatatga cattcaaatg gtaacgatcg ctatcccttc cttatcaaga
    16561 aaaaaattac gacgaatctt cgagttagtc gaatccgctc gtgtgaaagt caacacgatg
    16621 ccttctatag aagaacttgc ttcagggaaa attagcgtat caaaattaaa aacaattgac
    16681 gtagtggatt tactgggaag agatgaggta aagctggata ttgaatccat caaagatcag
    16741 atcacagaca aagtcattct tgtcaccggc gcaggaggat cgatcggttc agaaatctgt
    16801 cggcaaatca ttcaattcaa tcctgcaaag ctattgcttt taggacatgg ggagaactcg
    16861 atctacttga ttgaccgtga gttacgcacg catcatcaaa attgtcctac agaaatcgta
    16921 ccgatcatcg cagatatcca agatcgcgag aaaatcaatg agatcatgga acaatatcat
    16981 ccagatatcg tctaccatgc agctgctcat aaacatgtac cactgatgga atataatcca
    17041 aaagaagcag tcaaaaacaa tattttcggt acaaaaaatg tagcagaagc agctaaagca
    17101 gcaaaagtga aaaacttcgt catggtatcg acagataaag ctaacaatcc tccaaatgtc
    17161 atgggttcaa caaaacgaat cgctgaaatg attgtgactg gattgaatga agaaggctgt
    17221 acgaaattct cagctgtcag atttgggaat gtccttggtt ctcgcggtag tgtgattcca
    17281 gtattcagag aacaaattgc tcaaggtggt ccaatcactg taacagactt tcgtatgact
    17341 cgatacttta tgacgattcc tgaagctagt cgtttagtta ttcaatctgg cgcattagca
    17401 aaaggtggcg aaatttttat tctagatatg agtgaaccag tgaagattgt agacctagcg
    17461 aaaaacatga tccgattgag cgggtatagc gaagacgaaa tcgaaatcat tgaaacaggt
    17521 atccgtccag gggaaaaact atatgaagaa ctattgttgg ataaggaaag aaatgatgaa
    17581 gcagtttatg agaaaatctt tgtaggaaac atcaaaggct actcaataca aaaagtgatg
    17641 gattttgtta agagcctgcc gcaagacgat gaacaattgg ctaaagacat tgtcactttt
    17701 gcaaatgctt cgaataagta gggggagtaa agtgaaagca attattatag gagctggtgg
    17761 atttggcaaa gagattgctt ttctattaca aagtgtctct agatatgaat tgattggttt
    17821 tgtcgatgat tcgttaaaaa tgaaaaatca agaattattg ggaaaacctg tattaggaac
    17881 aatagattca ttaatagaat tagaagaaga gactgctatt tttctgggaa ttgcttcgcc
    17941 agatatcaag gagaaaatat atcaaaagat aaataaaaat aataaactga ttttccctaa
    18001 tttagttgca ccaagtgcac tagtgggcat aaatgttcag ttagggattg gcaatatttt
    18061 gatgccctat acgacttata ctgctgacgt agttttagga aatttcaata tgatcaatat
    18121 cggctctact atcggacatg atgctcaaat cggtaattat aattcaattt ttccaagtgt
    18181 aaatatttca ggacatgtta tattaggaga taaaaatgaa attggagtag gtactaaaat
    18241 aatacaaaat ctaagaattg gaaatatgaa tattataggt gcaggaagtg tagtaataaa
    18301 aaatattaat aataatacta aaaatgttgg cgtgcctacc aaagtaatag aaagatggga
    18361 ttaagttgga aaataaacga atattattag catctcctca tatgagtgag gaaggatatg
    18421 aacaagagta tgtaaaagaa gcatttgata caaattgggt tgctccttta ggtgcgaacg
    18481 tgaatggttt tgagaaagaa ttagcaaaat acgtgggagc tagtcatgct gccgcattgt
    18541 cttctggaac agcagctata catttagctt tgaaagcggc tggggtcggt aaagatgaca
    18601 tagtgatttg tcagtccttg acgttctcag ctactgctaa tccaattctc tatcaaggag
    18661 ctatacctgt atttgttgat agtgattacg aaacttggaa tatgaaacct gaacatttgc
    18721 gaacagcact agaaaaatat cccaacacaa aggctgtatt agtagtacat ttgtacggtt
    18781 tatcagctaa tttagacgaa attactaaaa tatgcaaaga atatgatgtt actctaattg
    18841 aagatgctgc agaatcatta ggaacaacgt ataaaggaaa atatactggt acatttggcg
    18901 actatggaat ttattctttt aatggaaata agataatcac aacttcaggt ggcggaatgc
    18961 ttgtatctaa tgacaaagaa aaggtggaaa aagtgagatt ttgggctaca caatctcgtg
    19021 agaaggcacg ccattatcaa cacaatgaaa ttggctataa ttatcgaatg agcaatatcg
    19081 ttgcaggtat cggtcgagga caattgaaag ttttaaatga aagaattgag aaaaaacgat
    19141 atattcatag tttttataaa aacgaattgt ctaaattatc aaatatacaa ttcatgccag
    19201 aaaataattg gaatgaacca aattattggt taagtgtcat aacaataaag ggaataaata
    19261 cgaatcatct tattgatgag ttagataaaa ataatataga agcccgccca gtatggaaac
    19321 caatgcattt acaacctgtt ttcaaaaatt atgattatat tggcgatgat gtagcagaaa
    19381 ctttatttga cttaggagta tgtttaccat cagatacaaa gatgacagat gaagatttaa
    19441 aatatgtagt atctacgatt aaaagagtga tcaatgatgc gtaaaaaaac tttctatcag
    19501 ctttttggga aaagaatttt agatatccta ttgtcaggaa ttgctctcat cgttttatcg
    19561 ccgataatct taattgtagg ttttttagta agaattaaac ttggttctcc aattatcttc
    19621 aaacaagaac gaccaggaaa aagtgaaaaa attttttcca tgtataaatt tcgtacaatg
    19681 actgacgaaa gagaccacaa tggagaatat ttacctgatg aaattcgact aactaaattt
    19741 gggaaaatgc ttagagcaac aagtctggat gagctacccg aactttggaa tattttgaaa
    19801 ggtgatatgt ctatcgtagg acctcgtcca ttattagtgg agtatttgcc actatatagt
    19861 gagaagcaac ggaaaagaca caacgtaaga ccaggattaa ctggactggc tcaggtcaat
    19921 ggaagaaatg ctattagttg ggaagaaaag tttgatttag atgtttatta tgttgataaa
    19981 attagtttct ttaatgatct tattattata attcagacat gtaaaaaagt aatcaagaaa
    20041 gagaatatta atacaatcaa taacaatacc ccagaaaaat ttaaagggag ttaaatacag
    20101 cagcttaatg caagttttgt gacttgcatt atatttaggt ataaatcaaa gcgagtatat
    20161 tagaattata taacatatgt taaaaaaacg catagctaat aaaagtatga ttttaaaaat
    20221 ttgtgtagag ttttaaatat actgtttcaa tacgagaaca ttataaaaaa gtataaggaa
    20281 aagagagtta tggcaaagta ttataataaa aaaattctta taatggcaaa caacgatgat
    20341 ggtctttacc gttttcgagg aatgctcata aaaaacttag tagatcaagg taatgaagtt
    20401 attgtatctc ttccaaaggg caagaatatt tctaaattaa ttgaacttgg atgtcaaatt
    20461 attgaaactc caatagatag aagaggaatt aatccaaaaa ctgactttaa actatttttt
    20521 aactattgta atattttgaa aaaaataaag ccagatatgg tcattgctta tactatcaaa
    20581 ccaaatatat acggtggatt ggcatgtcgg ttaagaaagg tttcttatgc attaaatata
    20641 accggattag gaacggcatt tcaaaaaaag ggggttttga aaagtcttat tgtatttttg
    20701 tatagattag cagctaaaaa atctaaggtc atatttttcg aaaattttga aaatatgaaa
    20761 acatttcaga ctttacaaat tgctaaacaa agcaaataca agctattaaa cggtgcagga
    20821 gtagatgtag aacactttag ttatttagaa tatcctaaag atgagcaagt tcatttttta
    20881 tttattggaa gaattatgaa agagaaaggt gtagatgaac tttttgaagc aatgaaaaac
    20941 ttatatagtg aagagaaaaa ttgtgtactt gatgtccttg ggtattttga agaagattac
    21001 ggcataaaaa ttgaaaaata cgaaaaagag ggatggttaa aatatcatgg atatcaatct
    21061 gatattcgtc cctttgtaga aagagcacat tgctttgttt tgcctagttg gcacgaagga
    21121 atggcaaata ctaaccttga atcagcagca tcagggagac caattattac aagtgatatc
    21181 ccagggtgta aagaagcagt tattgaatcg aagtctggtt atttatgtga aagaaaaaat
    21241 agttgtagtc tctataattc gatgaataaa tttctaaaat tatcatatga gcagcgcatt
    21301 aacatgggaa aaatggggag aaattatatg attaaaaaat ttaataagaa gaaagttgtc
    21361 gatgaaacaa ttaaatcatt atttagttaa aaagatatgt tttgcaaagt atgtgataga
    21421 aaagagggat tctgtttgag taacgtaagt aatccattag taagtgtaat catgggagcc
    21481 tacaattcag agaaaacaat tggaagagca attgattcaa ttataaaaca aacatattta
    21541 cattgggaaa tgataatttg cgatgatgga tcaactgatg gaactgtaaa aataatacgg
    21601 aaatatgtag aaaaggataa acgtattaag cttgttcaaa ataaaaaaaa tagtggatta
    21661 gcatattcat taaatcattg tttgaaatat tcatcgggaa aatatgttgc aaggatggat
    21721 gcagatgata ttagtttacc agaaagattt gcataccaag ttggattctt tgaaaaaaat
    21781 ccagaatatg acgttgtagg atgtaatcga atagtattcg atgaaaatgg tgattctgga
    21841 atacgaaaat caaaggaaat tccaactatt aatgacatgt ataaaggttc tccatttgct
    21901 catccgacaa taatgatgaa acgaagtgtt tatactattc taggtggtta cagggttaac
    21961 aaagatacaa caagatcaga agatttagaa atgtggtttc gattcttcaa tgaaggattt
    22021 agaggttata atttacaacg agtcttattt aaatatcaag aaacaagcga agattttcat
    22081 aaaagaagtt taagagcagc ttacggaata acaaaagtat tgttgagagg ttataagtta
    22141 ataggagtta gtaaatataa atatatttac gcatttaagc cgattatttc cgcaataatt
    22201 cctaagtata ttatgtatag atatcaagag aggagattac ataatactga agtattatga
    22261 tattgataaa attatgattt taacaattat tatttttgct gatatgctaa cttttttgtt
    22321 tatacgtaga tgtatatttg attactacca taaaatattt gcgtatttaa tgttaacagg
    22381 attcactttg tattcaggta tcggtattca atttcagcaa aataaaatat ctactccata
    22441 catatatcta atgcaatttg tgttatttat gattgtattt gtatttatga tttggtttag
    22501 tgaacggtta aaaaaaggaa aagcattaag tgattcagat ataatattaa atagtagcat
    22561 tttgattgta tttactataa tatacttatt cactttttta tatcaattga tttatagcgg
    22621 tcgttctata aatagtttta cagatattgt taacatattc attcattata gtccaacaac
    22681 ctttagtgag agattgtcta gaaaaaataa tgagatgtac gtgattatga cgaatcagat
    22741 acgttccatt gcagcaccat ttttttatat ttatttatat aaagtaa
//
LOCUS       DACDNO010000049        22444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDNO010000049 DACDNO010000000
VERSION     DACDNO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.049"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..209)
                     /locus_tag="IWI22_001929"
     CDS             complement(<1..209)
                     /locus_tag="IWI22_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9169317.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..622)
                     /locus_tag="IWI22_001930"
     CDS             complement(308..622)
                     /locus_tag="IWI22_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169318.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(690..1184)
                     /locus_tag="IWI22_001931"
     CDS             complement(690..1184)
                     /locus_tag="IWI22_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169319.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1204..1491)
                     /locus_tag="IWI22_001932"
     CDS             complement(1204..1491)
                     /locus_tag="IWI22_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169320.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            complement(1707..3098)
                     /locus_tag="IWI22_001933"
     CDS             complement(1707..3098)
                     /locus_tag="IWI22_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9169321.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3083..3694)
                     /gene="mobC"
                     /locus_tag="IWI22_001934"
     CDS             complement(3083..3694)
                     /gene="mobC"
                     /locus_tag="IWI22_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP9169322.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3698..3895)
                     /locus_tag="IWI22_001935"
     CDS             complement(3698..3895)
                     /locus_tag="IWI22_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169323.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(3886..6564)
                     /locus_tag="IWI22_001936"
     CDS             complement(3886..6564)
                     /locus_tag="IWI22_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169324.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSETVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(6551..6769)
                     /locus_tag="IWI22_001937"
     CDS             complement(6551..6769)
                     /locus_tag="IWI22_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169325.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(6845..7897)
                     /locus_tag="IWI22_001938"
     CDS             complement(6845..7897)
                     /locus_tag="IWI22_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9169326.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(8050..8376)
                     /locus_tag="IWI22_001939"
     CDS             complement(8050..8376)
                     /locus_tag="IWI22_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169327.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(8388..8588)
                     /locus_tag="IWI22_001940"
     CDS             complement(8388..8588)
                     /locus_tag="IWI22_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9169328.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            8663..9313
                     /locus_tag="IWI22_001941"
     CDS             8663..9313
                     /locus_tag="IWI22_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9169329.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            9368..10534
                     /locus_tag="IWI22_001942"
     CDS             9368..10534
                     /locus_tag="IWI22_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9169330.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            10951..14178
                     /gene="fss3"
                     /locus_tag="IWI22_001943"
     CDS             10951..14178
                     /gene="fss3"
                     /locus_tag="IWI22_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9169331.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            14290..14604
                     /locus_tag="IWI22_001944"
     CDS             14290..14604
                     /locus_tag="IWI22_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9169332.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            14617..14991
                     /locus_tag="IWI22_001945"
     CDS             14617..14991
                     /locus_tag="IWI22_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9169333.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            15001..15345
                     /locus_tag="IWI22_001946"
     CDS             15001..15345
                     /locus_tag="IWI22_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9169334.1"
                     /translation="MEINVEHILDCLDQFGKGELTGEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            15414..16430
                     /locus_tag="IWI22_001947"
     CDS             15414..16430
                     /locus_tag="IWI22_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9169335.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            17024..18898
                     /gene="ltrA"
                     /locus_tag="IWI22_001948"
     CDS             17024..18898
                     /gene="ltrA"
                     /locus_tag="IWI22_001948"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9169336.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            19024..19380
                     /locus_tag="IWI22_001949"
     CDS             19024..19380
                     /locus_tag="IWI22_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP9169337.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            19425..20567
                     /gene="dcm"
                     /locus_tag="IWI22_001950"
     CDS             19425..20567
                     /gene="dcm"
                     /locus_tag="IWI22_001950"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9169338.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            20557..20846
                     /locus_tag="IWI22_001951"
                     /pseudo
     CDS             20557..20846
                     /locus_tag="IWI22_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21102..22286
                     /locus_tag="IWI22_001952"
     CDS             21102..22286
                     /locus_tag="IWI22_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9169339.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            22301..22396
                     /locus_tag="IWI22_001953"
     CDS             22301..22396
                     /locus_tag="IWI22_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9169340.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         7215 a         3972 c         4061 g         7196 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 cttctaattc aaataaatct agtgacgaac taaaatcata aatcatgtca tattgaataa
      541 aaaaatcaag aaaaagttgc tcaaactctt ccttatttat cactaatgtt tcaaacggaa
      601 caactttatt ttcctcagcc attctcccag ttccattttc aaaaatcaca aaactgatat
      661 taccttaaaa actactaaca aaataaaact tataaagtaa atggcaaccc aactatgtaa
      721 ccattctctc tacgtactac gatttggtag tcctgccttt caatcggtaa ttcataatag
      781 taatttactg tttcttcttt gccactttca atatactctg gattatccaa tatcatatca
      841 tactcataaa gttcttcacc atccttattt tcaatcgacc attgttgtgg acgtatcgga
      901 ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg ttgtgcaaat
      961 tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc atctgcctga
     1021 taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc ttctggaaaa
     1081 tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag cactaaaaga
     1141 attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt tctcctccgt
     1201 tttttatttg ttttttagag aatacgataa cttttgatca atgtcatcca gcttttcaat
     1261 tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt taatgctttc
     1321 aggatcatta aatttattat ttgatggact agctaacact ggtaaattcg aaaccataac
     1381 acgaaacata tttgcctttg aaccaaaaac tcctttgttt tggttgtaaa aattgttgaa
     1441 tatttcttta gtttcttctg aagccgagac tttaaaatat ggatagttca tttttttctt
     1501 ccacctttct ttttttggga gggagtctgt agggagcttg ctccctcaga gatttaccca
     1561 ccatctgact tgccagaaag tagcctagcg tgagcggggc ggattgactg gcaagtagat
     1621 gggtagctgg cgtatgtcct aaaaactgct tctttccaag caactcttca taggcaaaaa
     1681 actggaaagt ccactaacaa gcccattcag aattccattt caaaattttg ttgccttttt
     1741 tgctgatttt caatcatttg ttgtgtgaat tcataatcct ttttagcacg atacattttt
     1801 acatcgtcat tcattatgcg actgatttga tagtgaatct gtttagcaga aggtgtatga
     1861 gaacaatttt caaaaccctt tgccggtaga aataactctt tctgatcagt ctttttaata
     1921 gaaattgttt tacttttcgc actacgtaaa gtttgaggat tcacatccgt atgaagttta
     1981 atcgtgtatt cttgatcatc ttctaaagac attgtattta caaaatgctt gtctacctct
     2041 cgcaagaagt catcatattt aatgaatttt tcatcgcctt cacggtcgct aaattgcttt
     2101 tgtgcgatgg aatctaattc cacaaacggt acatttaact caccagtttt gtaatttact
     2161 ccactaatta gcgacataac ttgctctctt tccatattaa aaaaattttt tatatgtgat
     2221 tcttctaaat tcttttcaag ttgttcagaa acagattcaa atgaatatct tttccctaac
     2281 tttcttgcac taaaagtacg ttcgacatac tgactttttt cttttctaga ttgtttatct
     2341 cttcctaaag taattaactc acctctacgc tcatagaaat ttacaccttg tttgtaaaga
     2401 acatagccaa gtttgttatt tgtttttgaa aattgataaa cttcaatgtt atattctttt
     2461 gccaaatgat ccgaaaaatc ttcaaaagaa ttcacttcgc atttttccaa cgttgcttcg
     2521 atcgcatttt gcacaaactc ttgataatta atttttccat atttcatctt agaaggatcg
     2581 tttgaataaa gattggttct cttttctaag ttttcatagt actcattatt cactgattca
     2641 agaccatgtt cattgaggac ttgatcgtta atttttcgca ttagttctaa atctttaggg
     2701 tcaatacgca accttttaca tgtttccatg ttaatattat taatgacaag atgactatga
     2761 aaatgatctt tatcattgtg acttgcaaca atgatttgcg aatttggaaa agctttctct
     2821 gcaaattcga cagctatttc gtgcatttta tcaatactta actctttaga agtactctta
     2881 tcaaacgaat gaataacatg aaacgcttgt gctatatctt tagatttatt catcttggta
     2941 cgtactaaat tcatttcttg cagtgcttcc tcactatcat cactacaatt tattcctgag
     3001 actgcaacac atttttcttt ttttgaagga ttagctgcat attgtaatag cgaggataaa
     3061 tttttcttgc gtcctttcga tgttatagta gctcccaaag ttgtttcacc tcatctttta
     3121 aacttgcaat aatctgaatt gagtgaatta aattccgatc aactttttgg gaatactctt
     3181 ctttcgtacc cggtctagtt tgcttttcta tccaatattc gaaatattct ttttctactt
     3241 ctgataattt aaaaaaagag ggggcactcc gattggactc caaaaaaatg tcatctggat
     3301 aactaatcat caactttttt gctatgctat tcgcaagttc atctttaaaa atgttgtaac
     3361 ggctagcatc aaaactaaaa tgtaggctct tttctttttt atcttcagat tcttctctaa
     3421 aatatttagc aatgatattt aatgccttta ccgcttgatt caaattgtta ccaatttgat
     3481 ttaactgttt ggttaaaact accctatcat cttcagctaa caacggcggt tcatatttga
     3541 gtccctttaa ccgctcacgg caaacgtctg ctctagaaat tcccctttgt tttgcaatca
     3601 atgtcagttc aagataagtc tttttatcga cacgaaaatt taaggcctca ctatgtttgc
     3661 gatttttttc gtacattttt tgtttatcat ccattgatta tcccctcaca tttttttcta
     3721 gagagcaaca tttgaaattg ctctctattt catttttcat ctttaattct tcttgaatca
     3781 tcagttcaat ataattagtg accgtgattt tttcatttgt tgcgttcttt actgattcac
     3841 ataattcgtc aagttctaag aaatcatgcg tactaatctt tagaattaat tgcatgacaa
     3901 tccccccctt ttatttctta ataaattatc gaaaagtgga ttaggatcat taagtgtatc
     3961 tctgaataac tcattgtctt cataagtgaa tcttcgtctt gattttgatc ccttatcatt
     4021 ttcacggatt tcactatcaa taatcactgt ttcatgattt gaatcaaatt ttttcgctga
     4081 ctttctgtct tcaacgatat tgattttttg tgttttttct ttcatttctc cactatcttt
     4141 ttgaatgttc tcccgaacat ctactgtttt gaatccttga ctacttgtag aactttgtac
     4201 attttctcgg acatctactg tttttggttg acttgcactg ctaccttgca cattctctcg
     4261 gacatccact gatttaaacc cttgactact tgtagaattt tgtacatttt ctcgaacatc
     4321 tactgttttt ggttgacttg tactgctact ttgcagattt tctcgaacat caattgtctt
     4381 agtaccttga gcatttgaag aactttgtaa attctctctt acatcaacat tccgtccaac
     4441 atgaccattc agtgaacctt gaatattttc tcggacatct actgatcggc caccatgact
     4501 acttgtagaa ttttgtggat tttctttgac taatttttct aatcctgtat ttgaattttt
     4561 agcttcttga attctttctt tggtcaacgc ttcttttccg tcaatattag cgcctgtccg
     4621 cccttctaca cgtttcctta acattatttc ctcgcgattc attgcttcag gcgtgtattt
     4681 cgcttcatga cgcttctgat taattaattc ttcagcagac ttagcttgac cgtgcatagc
     4741 atttttacgt tcttcaatat cttgttggac tttttgctga attgcgcttg caggattatt
     4801 attcgattca gcattatttt tcattgcttc acgcatacgt tcagaactag acataattct
     4861 atcggcattt tcaactttag caccatcttt tctatctgaa agacgtgttg gcacggaatc
     4921 agcatatttt ccagtttcat cttctttatt tttagctaac ataggtttgc tatttttatt
     4981 aatagcggac actgtaccat catctaaacc ttctttaaac ttattcttgg tatcattagc
     5041 tatatcacgc atactcttaa atggttttgt tgctctttca ctagccattg tagctccatc
     5101 tgaagctaaa ccggataatc cacgttcatg ggcatatccc aaagctcttc cagttttgcc
     5161 agcttttttt gatagagttt cgccgaaatt cttttctggt tttcgatctt ctccttctcc
     5221 gcttccttta gcacgattaa atccatttga tccttttcta aacagcattg cgcccattcc
     5281 aaaagcagaa gcaagttgcc catagccttc tttgagtcca atatcaacac caaagtatct
     5341 catgactgtt tcactgcctt tgattaaaac aaaaacggca cagatataag caattgtttt
     5401 tataaaaata tttgttgaga taccttgtcc tgagttcaga aaattgataa acatagcaaa
     5461 cattgataac ccaaatcctt gaaacccaac agttaagtaa cattgtagaa tatctgatag
     5521 aacaactttt gaacgctgtc ctgtctcaat atctgttgca aaaacaagaa cacctaacac
     5581 acgtttaaat gcaagttcta gtattgctgt aatgatcaca aacgcgctga acacataggc
     5641 gattgctaac gctgtcaacc caattgaaat cccccataga tttgcttggt atcggtaata
     5701 gcctgatttc aagctatcag aaaatgttga taagaagttg tcagaaaact ttgtagcaac
     5761 aaattcatta ttagaatttt caactaattc ataacgaaga ttgtccgcaa ttggattttc
     5821 tttttccaat ttatcaatca ctttatccgt taacacttgt gtgagatccg tctgttgaaa
     5881 attttttcgt gttaaattat ttcgatcctc cgttttatca atgcttgagt actgatctgt
     5941 tttattaata tatgctaaat ctgttactcc ttgttttatc aatgaccaag cgactccgtc
     6001 atctgaattt gtaatcgtag tagagtcctt ataaaatgtt ttagcaaatt gaataccact
     6061 agaaatcatg gttggcatta aaagtattaa tgctatagac atcactacgt tcattccaac
     6121 cgattttaaa tccactgttc cttttccagt aatcattttg taaccaacaa acatcaatga
     6181 aaggatcatt aatccaatga caattgtatt tacggctgat tgataaacat tatttagccc
     6241 tgtactttca atgaaattga acaaatccag cacatctgga atcatatttt gaatcgaatc
     6301 tgtcacagta aacatgcctt ttaccacagc ccatccgagc caaatcccga actgactaag
     6361 gaaatctcca ttgtactttt ttaggtaagg tctataaaga gaattattca aaaactctgt
     6421 ttgacttttt gtgtcatcaa accaattgaa aatactcgca tgagcaatat tacttgtaaa
     6481 aataatcata gctatcagaa caagaaaatt aacaaccaac caatttattt ttttattggc
     6541 aatctttttt tcaattattt tcatcgttac ctcccttagc ccattttctg aggctctcag
     6601 agagcttctg agccattttt tgattaatta tgacatgagt tacacaatta tcattttcag
     6661 ctcttaaatc gagaactata gtgttgaagc aaatagtctc gattaattcg agactatctg
     6721 tattttgatc tagacaccaa acttgatcta ttaaacgatt tttaatcaaa ataacccctc
     6781 ctatattcaa agttctcaaa tgaagtggag atacccctcc atttcatttg actcaattct
     6841 attcttattg cttatctatt ccttcgctga tttgcaattc aattagttct aacgctttat
     6901 tttttagaga agcaaagata taatcgtctt tttgtttgat acgatgatct gtttttgctc
     6961 ttcgtatcaa gtcacgtaaa gtcattgata gctgttctct aacatgatct acaaaatact
     7021 tgttattaca aatttcatag ataattttca gttgaatttt ttgttctttt tcagcagatc
     7081 taatcgcata tttgaatttt ttgtaccagt tgtcgaattg acgataatca ctattgctag
     7141 ccagttttaa gcaattcaca atactttctc cccatatttg tttaagaaga ttttcttcaa
     7201 taaatgtttc gatcaattct tcatctaatt tattattttt agttgaaagt gattcaagaa
     7261 gatcatcatt atgtgattct ttaatatctt tactatcttt ataagactta tataattcta
     7321 gttcattttt tgaactatca gcaaaattgt ttttgtctct tgttttcatt gatgtgtcgc
     7381 atagttcaaa ttttgaatta tcaacagaat attgatttgt tccatagttc aaattttgaa
     7441 ctatcaagtt tttttgtcta ttttcttgat gggttatctt attaatttta taaacatcat
     7501 ctttgcacag aacaggcttt aacaaataat aaaaatttgg gaacatttgc attttacttt
     7561 tattgaagcc attttttctt ttcacaagaa gtttaacttt ttgaagttca tctagtgctg
     7621 ataagattgt attttttgac attcccgaaa tttctacgag atttttgatt tcaaatatca
     7681 aaaaaatttt ttgattctca tcatacaagt tatttttcaa tgaaagtcta tttctatctt
     7741 ttagtaacat ataaactaac ttagaactta gtttcatttt cttatatttt tcatttacgt
     7801 ttaaaaaaat aggatattga tagtataatt ctgcatatat atcagaatta gaatagcctt
     7861 gcttttcaac taagctttca acttcttttt cattcattgt cacacctgct tctgatttta
     7921 tttgctataa tagattcaaa taaagttttc tcagaacttt attattgagt gttcccccga
     7981 tctgcaaact gacgggaaca ctctttttaa ttttttctac ttcatcaaat ttaaaaaacg
     8041 aggtgttttt taaatttgat gactcaatat ccattttttg cattcttctt ttgaaaacaa
     8101 ctccattttt tctaataaaa taactggcaa tcccattctt ttaaatttca aaaaagttgt
     8161 atatgataca ttcaaatatt tagccatctc ttttcctttt agaaatggtc tttctaatcc
     8221 ggtatcaatt cttgcttgat caatagcctc ttttgtcatt tcaaaaacat aatttttcaa
     8281 ttgattcgtt tgttgtttat ctaacattac tgtaattcct gcatccatta tttttctcct
     8341 ctcattttct atttcttcaa tcagccaact cgccaagttg tatttattta gattttcaaa
     8401 tcattaagta accaatttac caacttttga taagtagatg ctctaatact ttctttgctc
     8461 ccattttcga ttttgacata tgttttccga tttataccta tcaattttgc tgcatcgttt
     8521 ttagtaagta taagatcggc tctatgtctt cttaagcttc taatgaattg atcattaaga
     8581 gttatcattc agcgcctcct ttcaaattat tcactttgtg gtttttatct taataagcac
     8641 attttaaacc acaaaacgag taatatcaat taaaatatta ctcaatttgt ggttttcatg
     8701 ctataatagt ttggaattta ctataggggg cgttactttg aatagaataa aattttatat
     8761 tgattccagt aaaaagacca ttaaacaaat tagtacagaa actggtatta actatacaac
     8821 tcttacgaac tattaccgtg gtgagcgaaa tcctagaaat actgaaacat ggaatatcct
     8881 tgctgattat ttccatgttc ctgtaagtta tttgatggga ttagacgaaa caaattccat
     8941 agaacaacct aatcttgata gaataaaaac tgctcgttta gaaaaaaagg acaaaatagt
     9001 caagaatatt gccagtcttg actcagatta ttttactgaa ttatctaatc tatacttttc
     9061 catgacttct ttacttgaag tcaaacttca agaagatgac tccgactttc tttttttaaa
     9121 aagtattcta gaggactcct ttgaatacac gattctattt gccaaacaaa agaagtttat
     9181 taataaagct ttaaacttaa atatcgatga aagaaaagag ttagaatctg agttatttgg
     9241 caacttaacc caagtacagg ttaacaatct aaaaagagag ataacagctc gattaatatc
     9301 aaaacttcaa taaaactaat tattcaatca gccaactcgc caagttatca gattggagag
     9361 attagttatg tcaatacaaa agtacacaac aaaaagcggt gcacgatata gtgtccgctt
     9421 gtatgttcgt aaagatgaaa atggaaagca agaatactat gtaaaaaaag ggtttaaaac
     9481 tgaaaaagaa gccaagttac acgaagcaag aaaaaaggtc gagattgaag ataccggcta
     9541 tcataagcct tctaatgact tgtttataac agtgtatgaa gaatggttgc cttcttatag
     9601 gaatcgtgta caagaaacga catatcaacg tacaaaagac cttttccgct tgcatatact
     9661 accaaaattc gggaataagt ccatttctaa gattactcct gttcactgtc agaatgcagt
     9721 caatgaatgg gctttaaaat tcaagaatat caaacagctt aagtcttaca gctctcaaat
     9781 atttgagtat gccatttttg cagagctatt acaccgaaat cctatggcaa atacaaatct
     9841 accaaagcgg gaaaagaaag aaaatgaaaa ttacttttca ttagaagaac taaaagcttt
     9901 ttgggatatt ctcaataaag aagagccttt aaaacatatt ttaatcttcc agcttttgat
     9961 tactactggt atacgtaaag gagagctagc tgctcttcat tggtctgaca tttattttga
    10021 agaacagcta atgtatgtta gaaagagcta tgcaactatt cgaaatgaag agaagaatgc
    10081 aagaagaaaa actgtcagga ttcaaaaaaa tacaaaaaat atatcttccg aacgaatatt
    10141 accaattgat gcacaaacta ttgaaatgtt aaaaatctgg aaaaaagaac aagctcgaga
    10201 gcttttgcag tttggcataa atacaaatac aaaaaatcaa cttatcttta cctatgtaaa
    10261 tgcagacggt gacattaatc aacctttgca tgcggactat tcaaataata tattgaaacg
    10321 tttagagaag aaatataaat ttaagcacgt tactattcat ggattccggc acactcacgc
    10381 tactcttcta ctagaaggtg gagcaagtat caaagaaaca caagatcgat tgggtcataa
    10441 aaatgctgaa actaccctta acacttattc tcatgttact gaaaaagctc aacgaaatgc
    10501 gttagaaaaa ttcattgctt acactggttt ataatattgt cattaccttt ctcattacca
    10561 gaacgaaaaa aagacacctt ctcgcaatga gaaagtgtct gaaatcgttg gtataacaac
    10621 gattaacgtt ttgaaaactg tgaagcttta cgtgctttct taagacctgg ttttttcttc
    10681 gtatcattga ataagtttga aaagccttga tttataaagg tttttccgta aatatatttt
    10741 gttatatttg attaaatttg aaatgtttgt gttctaaaat gtgttctaat ttaaaggcag
    10801 tatatttcgt attttatatt aaatttttag gctcattcat tctcaaatcc attctattta
    10861 gtcaataaat attttaaaaa gactttataa acaaagatgt tcaagcgagc atcttatttt
    10921 tttgtgccta atttgaaagg agaacatttc atgtataaaa aacaaaaaaa gaaattatgt
    10981 gtattaacta ttttgagttt ggctgttcaa tcaattgtag gagcaattgc tcctacgatt
    11041 gcttttgctg atgaaattac ccatccacaa acggtaaccg tagaactaga tttagcacat
    11101 caatatgctg ttgaaggaac ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg
    11161 cattatgcag tgtacaacgg cgttaaacaa gatattttct gtgttgaacc aggagtcccg
    11221 atttacaatg agttcactcc tggatatgag aagaacccgt taccagatat gtccgataaa
    11281 gccaaattag tttccgtttt atggaaaaaa gtaggtacag atgcagatac tcatattgtg
    11341 gcacagaaga tgatctggca agaagttaat gggtataccc ttcactcaat aaaacgatca
    11401 gatggtagtg ccgtaaatat cgcagccatt gaggcaaaaa tcaatcaagc cattgccgat
    11461 tatcagaaga aaccaagctt ccataatagt acggctaaaa cagtattagg gcaatcgact
    11521 actgtgacgg atacgaataa tctgaatttg tcagagttcg atgaggttgt ggaaaatacc
    11581 gcaaacattg attatcgtgt gaatggcaat cagttgataa tcagcccaaa tgccaattca
    11641 aaagaaagtg gtgtgttgac gcttaagaaa tctgctggta ccggaacacc tgtcgcctat
    11701 aaaaaggctg gccaacagac tttaatggcc ggggcgatcg ataagccgaa cacttatacg
    11761 gtcaaaattg atgtggaaat ggagggttct ttgaagatta aaaaagttga taaagaatcg
    11821 ggtgatatta taccaggaac ggttttccat ttagactttg gaaaaacttt acccgcaaaa
    11881 gacgtgacca ccgataaaga aggcattgcg accttagatg agattcctca tggtacaaag
    11941 gtaaccatta ctgaaaaatc ggtgccggcg ccttatacga ttgataccac acccatgact
    12001 gccacaatta aagcaggtga aaccatttca gtgacttcaa aaaatacgcg agaaaaaggt
    12061 caaattattc tggataaaac cggaattgaa acagggagtg atctttggaa cgataattac
    12121 tctttagcgg gcaatacgtt tgccattcgc aaggatagcc caactggtga aatcgtccaa
    12181 gaaatgacca cggatgaaaa aggtcatgca gaaacaccaa aagagattgc caatgcgttg
    12241 gaattgggaa cctactatgt gactgaaacc aaggctagtc atggctttgt gaatactttc
    12301 aaaccagtaa aagtcgaatt gaattatgtc aatcaaacag tggctcttgt taccagtaac
    12361 gtaaaagggc aaaaccaaga aattactggt gaaaccactt tgacaaaaga agacaaagat
    12421 accggtcatg aaactcaagg gaaagctgag tttaaaagag ctgaatacac cttgtttact
    12481 gttaaggatg gaaaagcggt caaatggacc gagaacttta aaccagaatt gttgaaggga
    12541 acgaaagctt ctgatgaaac cgtaactttg actttagatg aaaagaatca agttgccgtt
    12601 aaacacttag cgattaatga gtattactgg caagaaacca aagcgcctga aggatatacc
    12661 ttggatgaaa caaagtatcc tgtatccatc aaaaaggtgg atgatagcga aaaaaatgct
    12721 gtgattactc gagatgttgc ggcaaaagaa caagtgattc gctttggctt tgatttcttt
    12781 aaatttgctg gatcaactac tggcactgcc gaaactggct tcaatgatct aacctttaaa
    12841 gtgtcgccat tggaaggaac caatgaaatc accggtgccg aagatgaagc gaccacagct
    12901 tataacgagc aattagggtt tgatggttat ggcaagtttg aaaatcttcc ttatggggat
    12961 tatttacttg aagaagtaga agccccagaa ggttttcaaa agattacgcc attagaaatc
    13021 cgttctactt ttaaagaaaa taaagaggat tataccaaga gtgattatat ctttactatt
    13081 accgaaaaag ggcaaaagca accgattaaa acggtaacgg tcccttatgg gaatctaacc
    13141 aatgagaatt tctcagtcag tttgaaccgt ttgatgcttt atgatttgcc tgaggaagaa
    13201 gatagcttaa cttctcttgc gacttggaaa gaaggagata aagaattgac tactcttgat
    13261 tctaccgagc tagtcgataa attgagctat aatctacatg aaatcaaaga agactggtat
    13321 atcgtagctc aagccatcga tgtagatgct acaaaagctg cccaagaaaa agataaaaaa
    13381 gccaaaccgg tggcgattgc ggaaacaacc gcaactttag cgaataaaga gaaaacagga
    13441 acttgggaga ttctgcataa attaactgtc gaacaagttt tagacaagac cattgtctta
    13501 ttcaactatg tgtatgaaag caaagaagcc tatgaagctg gtgatgagcc ggtagcgaag
    13561 gatgctagct tgaacaatca agcgcaaact gttaattgta tggtggaacg tcatgtttcc
    13621 atccaaacca aagcgcactt agaagaaggt tctcagacct ttactcatgg tgacgtggta
    13681 gatatgtttg atgatgtatc cattacccat gatgtactag atggctcaaa agaagctttt
    13741 gaaacaattc tgtacgcttt atttccagat ggtacaagta aagaaatttg gaaatctggc
    13801 aagattgatt acgaagtgaa tgacaaagaa tttaccaaaa ccgtacttgc ggaaaaagtg
    13861 gatacaagta aatatccaga aggtacaagt tttaccttca aagaaattaa ctatgacaaa
    13921 gatgggaata tcaatggaaa acacaatgaa gatttgaaag aaaaatctca aactttaacg
    13981 cccaaaaaag ttccgacaac accaagtaca ccagaacaat ctgaaatgcc aactgttcca
    14041 agtggctctc aagaatctag tcctacagtg aaggcattcc ctcaaaccgg ggagaaaact
    14101 tccaatgtgc tactgttcat tggctttagc ttgatctttg cgacggctgg ctattatttt
    14161 tggaatcgtc ggaactaagg tgataagatg aaacatgtaa atagtagaaa acaatacgcg
    14221 cgaccgcctc ctaaaaaagt ggtcgcgcta tttttgtatt caaaactaac aatgaaaaga
    14281 ggaaaaaata tggaattgaa atttgttgta ccagatatgg tcgagacgtt cggaaaacta
    14341 acatacggtg gtgaaggtga agttctatcg gaagggtatg gtcgaaatac tacaattgtt
    14401 ggtcgaagtt accatctgtt ttcaaataaa caacgggccg atgatattga agtggtggta
    14461 gctgcggaag ccggcgaaaa ggattttgac caagatcaac cactaaaagc tgtaaatccc
    14521 cacttaattg ccaaaggcta tgaaattgag aatcgtgggt ttaccgatta cgtgttgtat
    14581 gttgatgatt tagtgaaagc ataggaggaa aagaagatga gattaacaga aggtattgtc
    14641 gtagattcag gattaacctt tggcaagttg cgtttttccg cattacgtcg agaagtacgc
    14701 aagcaaaatg aggacggaac ggttagtaac gaggtaaaag aacggaccta taatttgaaa
    14761 tcctccgcgc aaggtcgaat gatccaagtc agtattccag caaatgtacc cttacgcgag
    14821 tttgcttatg atgcagaggt agagctggtc aatccaatcg tggatacggt cgccaattat
    14881 gtttttcggg aaggaaccac cgttaattgg tttatcaagg cagatgattt agtcttgaaa
    14941 cgacaactga atcaaggaaa acctacgaac caaaatgaag gaaagaaata ggtgaagaat
    15001 atggaaatta atgttgaaca tattttagat tgtctcgatc aatttggcaa aggtgagcta
    15061 acgggggaac aactcactaa cgcattaacc tatgatgaaa aaatgtttct gattatgcat
    15121 caagggttac tggaagtagg taacgatgcc aaagaagact ttgatgtact ggattggtta
    15181 ggggaagaag agtctttctt tatggttgtc gaggtaaatg aaagtttatg tcggcaggca
    15241 gaatctgttt tggaagaaat cgatgtggag atgccggatg ccatcgaaag atttttaaaa
    15301 caattagtgg aaacgaaaca acttcccgta gtagtaaatg actagcataa ctttttgaaa
    15361 ttgcccaatg aaatcctatt gggctttttc gactggaggt ggaaaccatc aaaatgtaca
    15421 aaggacatcg catacgcgca ggagatcaac acttgattta tcactttgtt cttggctggc
    15481 taatcttttt gttcattggt tggatgagcg tcttttattt acaagaactt agacaatttg
    15541 atatttctaa ggtatcattt tccactagtg aaattggctg gtctatgaag gatgtaatct
    15601 gcttgctagg aagtctcgct ctttcaggaa gcatgatgtt actttatata cacttttttc
    15661 aggatcattg gcgaagtctt tggcatcgac aaaagttagc tcggatgatt ctagaaaacc
    15721 attggtatga agtaaagcag actcaaggtg aaggtttttt caaagatttg aatagtagtt
    15781 ggaccaaaga gagtatcagt tacttcccca aaatttatta tcgaatgaaa gatggcttac
    15841 tttctattcg cgttcaaatt tcactgggaa aatatcagga tcagctattg aagttggaaa
    15901 ataagttaga aagcggactg tattgtgagt tagtagaaaa agaactcaaa gactcctatg
    15961 tggaatacac cttgttatac gatatgattg ccaatcgaat tgggatagaa gaaatagtgg
    16021 cagaaaatgg ggcgttgcga ttgatgaaaa atcaagtatg ggcgtatgat tccttgccgc
    16081 atatgttaat tgctggcggc acaggtggtg ggaagaccta tttcttactc accattattc
    16141 aagcactatt gaagtcagat gcagaattgt ttatcctcga tcccaaaaat gcggacctcg
    16201 ccgatttagg tacgataatg cctcacgtat actcccaaaa agaagaaatt tctgaatgtg
    16261 tggaagattt ttacgaacgc atgatagctc gtagtaggtc aatgaaagaa atgcccaact
    16321 acaaaacagg agagaattat gcgtatctcg gacttccgcc caacttttta atctttgatg
    16381 aatacgtggc ttatatgggt gcgaatcgtt ttcctacaag catcgaatag atgtgaaatg
    16441 gaagcctatt gagattatca atagtgacaa ctaatgtttt gagagatgga atgaaagcct
    16501 tcaagttgag ctgaaacatc cccgctaata ctcctacatg ctctaatcaa tgaagtagat
    16561 tggttttggt atgaagctag gtgaagtcgt ctgaaatctg cctaagtgct tgagcataag
    16621 gctgactata gggcgggcgg ctgaaaaaga taactatggt aagaatgtct gaaaatgact
    16681 gacgaatctg cgaatgtacg ggtctaaaag gctgatctta ttgaaagata ggaacatacg
    16741 gacgtatggt gagataccga caagtaaggt tccatgatat gaaaatcggg atagctaacg
    16801 aagaagctat aatttcacag gctcatagta gatacctaag gttatacatg atagctaaaa
    16861 acattggaac gatgtaagca gaaaactatc aattaaatga ctacttctag ataggtgcat
    16921 ataagtaata acgaaattgc tttgtttctg tgaaagtaag ggcacagtac cgatgaagcg
    16981 tctaacaaat gtggagggat agccctaagt ctatttaata acaatgaatg ttgaaacaga
    17041 tgaagttcaa ggatctggta agaacgtggg aaaatccgtt atggaggtgc cacgcttgaa
    17101 cacaaaaata cgttactggg aatattatga tctacaagaa acattcgatc agctatttac
    17161 tcaaagtaga aacgggaaaa agttttatca attatatgag ttaatcattt ctgaaaataa
    17221 tattctttta gcataccgta ctatcaaggc aaacaagggg tcttcgacac caggaacgga
    17281 ttccttcaca attgataact ataaggaaat gaaccaagca gagtttatcc atcttattct
    17341 cagtcattta gaaaattaca aaccaaaatc cataaagcga gtaatgatac ctaaacccaa
    17401 tggtgagaaa cgcccattag gaattccatg catgattgat cgaatgatcc aacagatgtt
    17461 caagcagata ctggagccaa tatgtgaagc taagttttat gaacatagct acggtttcag
    17521 accattgaga agtgcaaagc atgctctagg tcgcattatg tatctcataa acatcagtaa
    17581 aatgcactat gcagtagaca ttgatatcaa aggtttcttt gataatgtaa accaccgttt
    17641 actgataaaa caattatgga atataggaat ttgtgacaaa caagtcttag ctattctttc
    17701 aaaatctctg aaatcaccca ttcaaggaga aggtatcccg agcaaaggaa cgatccaagg
    17761 aggtatcatt tctccactat tatctaatgt tgttctaaac gacttagacc attgggtctc
    17821 aaagcagtgg catacctttg aaacaaagta tccctatact aaaggttaca ataaatttcg
    17881 tgctctaaga gatacaaatc taaagcaagg ctatattgtc aggtacgcag atgactttaa
    17941 aatcatgact aatgattatc cgacagcatt aaaatggttc cacgctgtca agctttacct
    18001 aaaagacaga cttaaattag atatttccaa tgagaagtct aaaatcgtca atttgcgtaa
    18061 atgtaagtcg gaatttttag gattcgctat ttgcgtgaaa caaaaaggga aaaaatgggt
    18121 ttgtaattca cgtatttcta acaaaaaaaa ggatcagatt aaagaagaga ttaaacaaag
    18181 aatcaaagat attcaaaaga gtcctacagc acaaaatgct ctcctgttta atgctctcgt
    18241 gctcggtgtt cataattatt tcaggtatgc cactcatatt aatttagatt tgaataggat
    18301 gaactacgtt ctttcattca ctacttacaa ccgattatca aattgtagta caagatgtta
    18361 ccccaacaaa gcttccccaa cttacaaaaa gttttactca acaacgatga aaacgtataa
    18421 agtagctgga gtctatttgt accctctttg cgacgtatct acaaaaacaa tttacggatt
    18481 taatacggag gatacaccgt ttactccgtt tggtcgtcaa agattggagc acaaaagcct
    18541 acagtctctt gtgttccaag aattaagaaa attgatggaa tcgaaaatac cgaatagaac
    18601 ggttgagtat ttagatagta gaatatctcg ttactcaatg aaatcaggca agtgtgagat
    18661 tacaaaacaa tttttgccag caaaagccgt tcactgtcat cactatttgc caaaaagtct
    18721 tggtggagac gacaaattcg ataacttgag aattattcat aaggatatcc atcttctgat
    18781 ccatacaaca aataaaatga taatcgatca ctacgtgcat gaactgaaac tggtgccaga
    18841 gcaaatagca aaaatcaacc tttatcgaaa gatgtgtaat ctgcaaaaca ttcaatagaa
    18901 aataaataga tggaacgccg tatgaggtga aagtctcacg tacggtgtgg agcgggggaa
    18961 aagatggaga ttatatcaaa ttcttaccta tcgctataga tgttaacgac aaaagaaagt
    19021 gcggtgattt tgaataagct taaacaaatc gtgatgttag gccgtcaatc tggtttcttt
    19081 ctgattctag cttgtcaaag gccggatgcg aaatatctag gagatgggat tcgcgatcag
    19141 tttaacttcc gtgtggcttt gggtcgtatg agtgagcttg ggtattccat gatgtttgga
    19201 gaagtcgata aaaatttttt tatgaaacgc atcaaaggac gaggttatgt agatacaggg
    19261 ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa aagggtatga tttcttaagg
    19321 gaaattagca acattgttgg attaaccgtt catacggaaa gagaaaataa tagtgtgtag
    19381 cctaggaata tcaataatcc tgggcttttt tattgaggtg atttatgaca tttttagatt
    19441 tattcgccgg gattggtggc tttcgcttag gtatggaaca agccggtcac cagtgtatcg
    19501 gtttttgtga aattgatgaa tttgctcgga ggagttataa agcaatccat gatacaagga
    19561 aggaggtgga aatgcatgac atcacaagtg tatcagacga atttgttcga tccctcggac
    19621 cagtggacat cctttgcggc ggctttccgt gccaagcttt ttcaattgcg ggaaagcggc
    19681 aaggattttc cgatactcga ggtactttat tctttgaaat cgctcggttc gccactcttc
    19741 tccaacctaa gtttctattc cttgagaacg tccggggatt gctcaatcac gaaggagggg
    19801 ctacgttcga gacgatcctc cgaacgttgg atggattggg gtatgatgtg gaatggcaag
    19861 tgcttaactc aaaggcctac gttccccaaa gccgtgagcg gatcttcctt atcggacatt
    19921 ctcgagatgc atgtaccgaa caagtatttc ctatcattgg atcgtcttca acatctgatc
    19981 aaaacatcag aaacttggta aatatcaatc cttccaatcg aggaatgggt ggccaagtct
    20041 atggttcaga tagcgtagca ccaaccttga ccggtgatga aggaattaaa attgccttac
    20101 cggtaaagga tgggatttct gtagcgggta tgttgtcggg aaattttgaa caaggcaata
    20161 ggatttatga ggtaaccgga acagccccca ctttgtcaac gaaacaaggg ggaacgaaga
    20221 ttatgattcg gaaaaatgaa acagaatatc aagaggtgaa gccaggggac agtgtgaacc
    20281 tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacaaagt gtgcatacct
    20341 tactaacagg agaccaacaa gcggtagtca cggatcagta ccagattcga aagttaacac
    20401 ctagggagtg ctggcggctt cagggctttc cagattgggc ttttgatcga gcttctcaag
    20461 tcaattcaga tagtcagttg tataaacagg ccggaaactc agtaacagtt ccggtcattt
    20521 ttgatattgc tagaagatta aagggggtaa aagcatatga tttatgacga gttgattgga
    20581 gaaatctact gggtgattgg caagattcag gcagatccga aattggaaga agaactgtac
    20641 cggctcaatt ttgatattcg caaaaacggg gtgaaagtcc aggtgattcg tatgtagtgg
    20701 atgaggaaac ggatgctcga atggagctca atcaagtcat cggtgaattg gaacgaattg
    20761 cggaccttgc caaagaacct gatatccgcc aatacttgtt tgagataaaa gcagagttgg
    20821 aaattgaggg tatcaccgcg gagtaatagg gaaataaccc actgataggc actgaccgcg
    20881 ggggatttgc gaagcagaaa tcctccgtgg tgctgtgcct gttcatggac ggcggcagcc
    20941 ggccatgatg tctcaccccc cgtatctaac aggggggtac aaaaacacaa aaaacaagca
    21001 agtaaccata aaaaatgctt gttgtatcaa ggatattaag taatttgtat aggtgttaac
    21061 tcatttggaa aagttaacac cttgaatttg gaggtgaaaa cttggcgcaa cgaaatttag
    21121 attatcggtt attaaaagaa cgacgcaacg aatatggcgt ttcacaaaat aaattagcta
    21181 ctgcttgcgg acttagccgc ccatacctaa atcagataga aaacggtggc gtaatcgcct
    21241 ctacaaagac catgagaaaa atttttgatc aactggaaag tttcaatccc gatttacctt
    21301 taacgttact atttgactat gtaaggattc gctttcccac aacggatgtg cggaaaatta
    21361 ttcaagaaat tctccatttg aaattcgagt atatgcttca tgaagattac gccttttact
    21421 cctatcaaga acaatatgtc atgggcgata ttgtagtgat gttatctcat gaagaagata
    21481 aaggtgtcct tttggaatta aaaggtcgtg gttgccggca gtttgaaact tttttactcg
    21541 cccaaaagcg tagctggtac gacttttttg aagattgtct aaaagccggt ggcgtgatga
    21601 aacgcttgga tttagcaatc aatgatcgag tggggctact ggatattcct gatttaacga
    21661 aaaaatgtca gaaggaagaa tgtatctcct tgtttcgtac cttcaaaagt taccgttccg
    21721 gggaactgtt gaaagctgat gaaaaggatg gtatgggcaa taccttatat attggcagcc
    21781 tcaaaagcga ggtttatttt tgtttgtacg aaaaagatta tgaacaatat atcaagttag
    21841 ggattccatt ggaccagacc gaaacgaaaa atcgttttga gattcggttg aaaaatgacc
    21901 gtgcttatta tgcgattcaa gatttgttaa aaggtcgtag cattgaaagt accacgtttt
    21961 ccattatcaa tcggtatttg cgatttgcgg ataaagtaga tggaaaacgg cgaaccaatt
    22021 ggcccttgaa tgaacaatgg ggtcgtttta ttggacggaa tcggaaagag attcaattaa
    22081 cttctgaacc gaaaccgtat accattgaac gaacattaaa ttggctaggt agacaagtcg
    22141 cccctacgtg gaaaatggct aaggagttgg atcgattgaa tcaaacaacg tatatccagg
    22201 atatggtaca gaatgcgcga ctctcggacc ggcataaaaa gattttggag caacaaagta
    22261 tggcaatcga aaacttgatt gtatgaaaag aggaattttg atggaaattg taaaagatat
    22321 gttattggta cttggtggca gtatgcttgg tgttaccgtg atgtgcttga tgaatgcaag
    22381 tgcagcagcg gattgagcaa tggaaaagaa aggaaagtga caaaatgaat ttcggacaaa
    22441 attt
//
LOCUS       DACDNO010000050        21393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDNO010000050 DACDNO010000000
VERSION     DACDNO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.050"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IWI22_001954"
     CDS             185..1183
                     /locus_tag="IWI22_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9169341.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IWI22_001955"
     CDS             1295..1804
                     /locus_tag="IWI22_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9169342.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IWI22_001956"
     CDS             complement(1834..2493)
                     /locus_tag="IWI22_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9169343.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IWI22_001957"
     CDS             complement(2495..3259)
                     /locus_tag="IWI22_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9169344.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IWI22_001958"
     CDS             complement(3267..3944)
                     /locus_tag="IWI22_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9169345.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IWI22_001959"
     CDS             complement(3981..5366)
                     /locus_tag="IWI22_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9169346.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IWI22_001960"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWI22_001960"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWI22_001961"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IWI22_001961"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9169347.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IWI22_001962"
     CDS             6305..7162
                     /locus_tag="IWI22_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9169348.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IWI22_001963"
     CDS             complement(7243..7578)
                     /locus_tag="IWI22_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169349.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IWI22_001964"
     CDS             complement(7645..9015)
                     /locus_tag="IWI22_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9169350.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IWI22_001965"
     CDS             complement(9093..9629)
                     /locus_tag="IWI22_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9169351.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="IWI22_001966"
     CDS             9938..11419
                     /locus_tag="IWI22_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9169352.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTTKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHKLYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="IWI22_001967"
     CDS             complement(11522..12229)
                     /locus_tag="IWI22_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9169353.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="IWI22_001968"
     CDS             complement(12308..12646)
                     /locus_tag="IWI22_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9169354.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="IWI22_001969"
     CDS             complement(12757..13719)
                     /locus_tag="IWI22_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9169355.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGL
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="IWI22_001970"
     CDS             complement(13734..15065)
                     /locus_tag="IWI22_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9169356.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="IWI22_001971"
     CDS             complement(15073..15753)
                     /locus_tag="IWI22_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9169357.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="IWI22_001972"
     CDS             complement(15972..16451)
                     /locus_tag="IWI22_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9169358.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16578..18257)
                     /locus_tag="IWI22_001973"
     CDS             complement(16578..18257)
                     /locus_tag="IWI22_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9169359.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18805..20307
                     /locus_tag="IWI22_001974"
     CDS             18805..20307
                     /locus_tag="IWI22_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9169360.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20320..20982
                     /locus_tag="IWI22_001975"
     CDS             20320..20982
                     /locus_tag="IWI22_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169361.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(21083..21286)
                     /locus_tag="IWI22_001976"
     CDS             complement(21083..21286)
                     /locus_tag="IWI22_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9169362.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         6354 a         4435 c         3635 g         6969 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaac caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca taaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag agccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctgtcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc
    16621 aagatcactg ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg
    16681 tcttgcttta atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt
    16741 tcaatcatat tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc
    16801 cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc
    16861 gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata
    16921 tcacctatgc ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc
    16981 atacgcttgt tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat
    17041 ggcatcccta ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga
    17101 actggcacaa aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg
    17161 gcatggccag aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa
    17221 atgatatctt ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata
    17281 taaaccaaat ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc
    17341 agagacttga tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca
    17401 tagtttttca tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct
    17461 aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt
    17521 tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa
    17581 gcgacgatga tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg
    17641 gcttcagaag ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag
    17701 actccttctt tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc
    17761 gtttggtcaa atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg
    17821 atccccactg aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca
    17881 aagtctattt ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca
    17941 ttacgactga cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca
    18001 gaaatttttg acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa
    18061 aagacaccag ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg
    18121 tcgatcccca acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc
    18181 tcgcccactt ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata
    18241 attttaatgg tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga
    18301 ttttcttctt tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt
    18361 ctcaacttat ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat
    18421 gggcatttta ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc
    18481 cagaatactt atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc
    18541 gctagtatca gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg
    18601 atttcttttg ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat
    18661 tttaccacat tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga
    18721 tggtgtgcaa acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg
    18781 catctactag gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa
    18841 gtaaaatggg gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg
    18901 ctagctggcc tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa
    18961 gttggagcaa cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa
    19021 acttcttcaa gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca
    19081 ccagtttcct taacggacgc ggaaaaaata gctgaattag ataatgtaaa gagttattcc
    19141 tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat
    19201 tcaagtgaag acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc
    19261 gggaatccgc aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa
    19321 aatagtaatt tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc
    19381 gacaaaggca caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca
    19441 gtcggagata ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg
    19501 atcaaaggaa tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc
    19561 atgaatccgt cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca
    19621 tctgacgata acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat
    19681 gagtttctca aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat
    19741 gattccatgt atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat
    19801 gttgttttac tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc
    19861 attcgggagc gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa
    19921 atcattctcc aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct
    19981 tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca
    20041 gaaaccacga cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa
    20101 gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct
    20161 gatcaagtca gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga
    20221 gtagcatttg gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta
    20281 aacccaagaa aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg
    20341 ggattttggt atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa
    20401 gctggaaaaa tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg
    20461 cttatttctg gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg
    20521 aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac
    20581 aacttgcttc cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag
    20641 accaagcaag agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat
    20701 gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct
    20761 atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat
    20821 ctagatgaaa caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa
    20881 aatcgctgta ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc
    20941 tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca
    21001 aataaaaaat ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga
    21061 ttcaattagt aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga
    21121 cttcgtcgcc taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc
    21181 cttctgtttc tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga
    21241 ctactttatc tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc
    21301 ttagtttgtt tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa
    21361 cgctcgtttt ctaaaaaaga tagttagagc tgt
//
LOCUS       DACDNO010000051        20360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDNO010000051 DACDNO010000000
VERSION     DACDNO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.051"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            292..1431
                     /locus_tag="IWI22_001977"
     CDS             292..1431
                     /locus_tag="IWI22_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9169363.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IWI22_001978"
     CDS             complement(1498..2073)
                     /locus_tag="IWI22_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9169364.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IWI22_001979"
     CDS             complement(2078..3052)
                     /locus_tag="IWI22_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9169365.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IWI22_001980"
     CDS             3335..4303
                     /locus_tag="IWI22_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9169366.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IWI22_001981"
     CDS             4416..4694
                     /locus_tag="IWI22_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9169367.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IWI22_001982"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IWI22_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9169368.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IWI22_001983"
     CDS             5160..5906
                     /locus_tag="IWI22_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9169369.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IWI22_001984"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IWI22_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9169370.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IWI22_001985"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IWI22_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9169371.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IWI22_001986"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IWI22_001986"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9169372.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IWI22_001987"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IWI22_001987"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9169373.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IWI22_001988"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IWI22_001988"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9169374.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IWI22_001989"
     CDS             10643..11185
                     /locus_tag="IWI22_001989"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9169375.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IWI22_001990"
     CDS             11208..12764
                     /locus_tag="IWI22_001990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9169376.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IWI22_001991"
     CDS             12778..13680
                     /locus_tag="IWI22_001991"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9169377.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IWI22_001992"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IWI22_001992"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9169378.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IWI22_001993"
     CDS             15123..15545
                     /locus_tag="IWI22_001993"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9169379.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IWI22_001994"
     CDS             16014..16244
                     /locus_tag="IWI22_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9169380.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IWI22_001995"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IWI22_001995"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9169381.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IWI22_001996"
     CDS             17655..17831
                     /locus_tag="IWI22_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9169382.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IWI22_001997"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IWI22_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9169383.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="IWI22_001998"
     CDS             18326..18709
                     /locus_tag="IWI22_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9169384.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="IWI22_001999"
     CDS             18878..19762
                     /locus_tag="IWI22_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9169385.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IWI22_002000"
     CDS             19879..20112
                     /locus_tag="IWI22_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9169386.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IWI22_002001"
     tRNA            20200..20280
                     /locus_tag="IWI22_002001"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="IWI22_002002"
     tRNA            20287..20358
                     /locus_tag="IWI22_002002"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta
//
LOCUS       DACDNO010000052        20276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDNO010000052 DACDNO010000000
VERSION     DACDNO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.052"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            42..197
                     /locus_tag="IWI22_002003"
     CDS             42..197
                     /locus_tag="IWI22_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169387.1"
                     /translation="MVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLLNL
                     FNRIVGA"
     gene            210..713
                     /locus_tag="IWI22_002004"
     CDS             210..713
                     /locus_tag="IWI22_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9169388.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            840..1877
                     /locus_tag="IWI22_002005"
     CDS             840..1877
                     /locus_tag="IWI22_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169389.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1892..3589
                     /locus_tag="IWI22_002006"
     CDS             1892..3589
                     /locus_tag="IWI22_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP9169390.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3582..4043
                     /locus_tag="IWI22_002007"
     CDS             3582..4043
                     /locus_tag="IWI22_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169391.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            4056..4916
                     /locus_tag="IWI22_002008"
     CDS             4056..4916
                     /locus_tag="IWI22_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169392.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4954..5343
                     /locus_tag="IWI22_002009"
     CDS             4954..5343
                     /locus_tag="IWI22_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9169393.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5330..7777
                     /locus_tag="IWI22_002010"
     CDS             5330..7777
                     /locus_tag="IWI22_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9169394.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7782..9890
                     /locus_tag="IWI22_002011"
     CDS             7782..9890
                     /locus_tag="IWI22_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9169395.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9887..10891
                     /locus_tag="IWI22_002012"
     CDS             9887..10891
                     /locus_tag="IWI22_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9169396.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10910..11821
                     /locus_tag="IWI22_002013"
     CDS             10910..11821
                     /locus_tag="IWI22_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9169397.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            12008..12295
                     /locus_tag="IWI22_002014"
                     /pseudo
     CDS             12008..12295
                     /locus_tag="IWI22_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(12483..12698)
                     /locus_tag="IWI22_002015"
     CDS             complement(12483..12698)
                     /locus_tag="IWI22_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169398.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12957..13124)
                     /locus_tag="IWI22_002016"
     CDS             complement(12957..13124)
                     /locus_tag="IWI22_002016"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9169399.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(13469..14908)
                     /locus_tag="IWI22_002017"
                     /pseudo
     CDS             complement(13469..14908)
                     /locus_tag="IWI22_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15456..16127)
                     /locus_tag="IWI22_002018"
     CDS             complement(15456..16127)
                     /locus_tag="IWI22_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9169400.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16476..17927)
                     /locus_tag="IWI22_002019"
     CDS             complement(16476..17927)
                     /locus_tag="IWI22_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9169401.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(18039..19382)
                     /locus_tag="IWI22_002020"
     CDS             complement(18039..19382)
                     /locus_tag="IWI22_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9169402.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(19516..>20276)
                     /locus_tag="IWI22_002021"
     CDS             complement(19516..>20276)
                     /locus_tag="IWI22_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9169403.1"
                     /translation="DGTKDTVKVPVEVTDNRSDADKYNPEGQKVTTDLNKEPDASEGI
                     KNKEDLPKGTTYTWKEKVDVSTAGNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQ
                     VDPITEGDQIVTGKTEPNAEVTVTLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTA
                     TDESGNTSEPTNVVVSSNEKDSEKAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPK
                     TGEIDSNIFTISGGLILLGTLGLLGYKNRKKENE"
BASE COUNT         6529 a         3441 c         3772 g         6534 t
ORIGIN      
        1 aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt
       61 ttttaggatt taaacgggag ttttccaaac tgattggctt cttggtgatt gctttgattg
      121 cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cctactcaat ttgtttaacc
      181 gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat gcgtgtttat attgcgaacc
      241 ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacagatt ggattaaatg acgaatatga agagtacgcg attcatgatt
      361 atgaactgcc ttttgagatt gatgagtata cgccaattga agagattaat cggttgtgta
      421 acttagcgga ggaactagaa ggaacgccaa ttggtgaagc tgcttctgag attcaacacg
      481 ctttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatattgtc tattatccag
      541 attgcgatga tatgagtgat gtggcgcatt acttgattgt agaagctggt gattttggcg
      601 aagttccaga acaattgaag ccgtatattg attttgatgc ttgcggtcga gatttggaga
      661 ttggaggaaa ttatttggtt actagtcgtg gagtttttga gtatccagaa tgaatcaaac
      721 actatatata gtgtttgatt agtatatgta agcaaaaaaa cacaatatat agagtgcaaa
      781 ctgaagggta atgtgatata atatttgccg aagaaatatt tttgaaatga ggtaatatta
      841 tggcaaatat tcaaacaagt tttattgatt tccataattc aattagattg gacgtagaag
      901 acaatacgct acttaaggac tataaagacc aagtaattga tggattaaaa gattacttac
      961 ctgatgatgt gaagtttgaa acttttttac agggaagcta ttctgtttat accggaataa
     1021 aaagctgtga tgaaaaaatt gattttgaca tcgatattgc agttgctttc gaaattgatc
     1081 atactgttta tgaagatcca agggagccta aactgtgggt aaaagaggcg ctagttgaaa
     1141 tttttcctaa tgctcaagtt aatttaaaag taccttgtgt aactgctaca tttactggaa
     1201 aaaagacaaa aaaaaatgtg cacgttgatg ttgcggttta tgctaaagaa gatgaaaatt
     1261 atttcttagc aaaagcaaaa gaattcagtg ctcccgaaaa ccgatgctgg gaggaagcgg
     1321 atcccaaagt attaaaagag aaaattaata gtcatgtagc agattcagat gatcgaaaac
     1381 aatttcgaag atgtattcgc tatttaaaaa gatggaaaga taacaatttt aaccaagaat
     1441 ataaaccaac aggaattgga cttacaatta atgtgatgga tacgttccta gttaataagt
     1501 ccactgattt tttaactaga aaagttcagt ataatgatat ggaatgtatg aagcagattg
     1561 tttcttcgct gaaagattca tttgtgtatg agtacagtga aacagatggt tggcattatc
     1621 gtctacatgc atatcttcca gtgaagccta acagtgacac ctattcaaaa atgacagtga
     1681 atcagatgag tgattttaaa aacaaacttt ctaaattata tgatgatttg attttcgcta
     1741 tcgatactga agatgagtat gaagctacaa aacgattaaa taatcaattt ggggaggatt
     1801 ttttaattat ttcagaagaa gaagttacag aaaaaaactt aagaaatgca tttgttacag
     1861 actatccgag tgcttaggtt ggtgttaaag attggattat actgatattt taaaagaaaa
     1921 gctaacagat gttgtgcgtt tattcgatgg taattactat ggggattttt catttgctac
     1981 aattggaaag ttgcgtttaa aaattgaaat accaaaacat tttccattgg tacttcctag
     2041 gatttacatt cataattttg atcatttagg actttaccta ccacatgtag aaaaagatgg
     2101 gttggtatgt ttttcttcag atataaatta tttagtagat gaagaaaatt cagaattggt
     2161 tttattgcag tgtgttaata aaactataaa aacaattgag gaaggtttga aagggttaaa
     2221 taaaaatgat tttcgaaaag agtttgttgc tttttgggac aaacaagaga atcaaatttc
     2281 gttattctac ctttgtgaac cagcgttgtt tatcaagtca gttgatacaa taatgtacgg
     2341 taagtctttg gttgctttgg ataaagagaa cgaattacaa ttaaatgcag cgagtagatt
     2401 gtttaatgga gagaaagaag atactcatga attagatgca tactatgttc caattagaaa
     2461 caagaataat attatgcccc cgaagaataa taaagcatgg tcaaagaagg atattttaca
     2521 aatagtaaaa aaaaatgtaa caggtgcaaa taaaagattt tttttaaaac ttgaaaaaag
     2581 aaagaataga cataatgttt tacttattct agatatccca attgaagaaa agtcaaataa
     2641 tgtcataatt ggatttcttt ttacaaaaca atctggatct ataaaagatt ataagggtac
     2701 gcctaggact gtattagttc agcgtcatga tgaattatat ttggtgaata gaacgagcgg
     2761 agaaaactat ggattgggtg ctaatatagc tattgttggg gtagggtcag ttggcagtaa
     2821 aattgctgaa aatcttggtc cattaggtgt gagaaagatg acactcatag acaatgattg
     2881 ttttcaagct gaaaatttgt atcgaaattc acttggtgca gatagtttaa aaaatgcccg
     2941 atctcagaat aattttttta aagttgattt aatgcaggag gagctcgaaa aaaaattccc
     3001 atacctttgt gttgaagtgg agtcaatgaa cgttttagac ttaattagta tgcaacctgg
     3061 ttttttcaag gaatatgact atgtttttgt ttgcatagga gatacaatgg caagcctggc
     3121 tttaaataat tatttttcca aaagtagtca aaaagttttc tattcttggc tggagccata
     3181 tggcattggg ggacacactc tatttataaa ttattctcta aatcaaaaag gttgtttcaa
     3241 ttgtctttat acagattcaa actctaaaaa taggtgttca aatagagctt ctctggtaaa
     3301 agaaggacaa atttttgaaa ggactttagc gagttgccgc tcaaaattta taccatataa
     3361 tgctattgcc tcgacggaat gtgcaattaa tgcaataaaa cttttccata aagtcaatca
     3421 aaataatatg accaatagta ctcttttttc atggatagga gatgggagta cttttgagaa
     3481 tttaggatat caatatagcg acagaaatac ttttcttaat ggagtcaata agtttgaaga
     3541 aagattcttt tctaaatatt gcaaagtatg tggggagtta gatgattaaa ttttcgtgtg
     3601 accgtattgt cattgaagta agggatgaag ttcttaagat atttgaacaa tatcggcagg
     3661 gaaaggatga cttgcctgaa gctggaggag ttcttcatgg aaaaagactt aagaatggga
     3721 gcataataat taccgatata acaacacctc aaataggaga tatacaaaaa agattactct
     3781 ttaaaaaaaa ccgagaaatt catcaacagc tttctaatga gaaatggata gaatcaaata
     3841 agttttctat tgcattggga gaatggcata ctcatcctga agctgtacca actccatcaa
     3901 atgttgataa gaaaagctgg agactgaacg tgagcaagca acatgatgat agagtttatg
     3961 tttttattat cgttggattt tctgatttga gaatgtggtt actgtcaaaa aaatcagata
     4021 ttaggcaggc tataaaacta taaaggagtg attaggtgaa atcacaaaag aatatttggt
     4081 ggtgggggtt tttagtagga aaggtaatga taccagccct tctcttaatt tctctaccga
     4141 tgattacttt tttctttacc aataaaaaaa tgagtaaaga agccgtgatt tttttctttg
     4201 gagaccaaaa agctaccttt attgaaacgt tagtaacatc tcttcagctt aatttgaatt
     4261 acattttttc aattattatt gttatatcgt tattgaattt ttttaaaaaa agaaattcag
     4321 gaaaggtgtt taactctaat ggaaatgtgt actacaacta tttttatttt gtattttggg
     4381 tagcagcaac tttacttggt tatgataaaa ttcagattgc aggaattcct atacacatgc
     4441 aatataagtt ggttttgagt ggaatttttt ctgaggtgtt gccagatata tacgatgatc
     4501 attacgacag tgatggaaca tgtaaagtaa gcattgaaaa agaaaatttc gatgacatcg
     4561 atggatatga ctctgtgaac ttactgatta ttgatactta cgatataaaa atgagtgaat
     4621 tatctatgga aaatcaaaca tatcctacga taattgttag agggaattca atagatggag
     4681 ttcgtaaagt taatagatct ttaatcttgg aaataaaaaa aacaatggat gaaattcaaa
     4741 aaagtgactt taaaaaagta tttgttgctt caacatctaa tccaaaaaat agtatcaata
     4801 ttattaattc atcgtttaga tttttcggtc gatcacgtag gtttaaactt tatgttcttc
     4861 aaaaggatta tgcaagcaat ggaaagtata gtaagaaata tagaattttt atttaagaag
     4921 gaatttttcc ttctttttat tttggaggaa actatgaaaa aaataaaaag ctacaccagt
     4981 atttgggcag ttgaaaaagt catttatgcg attaatgact ttcagctgcc ttttcctgtg
     5041 acctttaacc agatggcttg gtttgtttta tccttgatgt ttgtcattgt atttgcccat
     5101 gtgcctccgc tatccatgat agagggggct tttttaaaat acttgggcat tccgatagcg
     5161 gtcacttggt tcatgtcgca aaagaccttt gacggtaaga aaccattggg atttttacga
     5221 tcctttatca gctatcactt acggtttaag gtcacttttg caggaaagaa agttaaagaa
     5281 cagaagaaac gatgggacga accaatcacc ttggtaagga gtgtgaatta tgtacccgat
     5341 taagtatatt gaaaataatc ttgtctttaa ccaagaaggc gagtgttttg cttactatga
     5401 attagtgcct tacaattact cctttttgtc acctgaacag aagtatcaag tacatgataa
     5461 ctttcgccaa ttaattgcgc aaaatcgtga aggtaagatc catgccttac aaattgccac
     5521 agaaagtagt attcgtgcca cacaagaacg ttcgaaaaag gaaattaccg gtagactcaa
     5581 agaagtagca aagcaacgaa ttgatttaca gacagatgca ttagtatcca tgattggcga
     5641 tagtcagatt gattatcgct tctttattgg ctttaaactg atttcgacag atgaagaggt
     5701 caatctaaag aatctcagga aatctttctt ttctggactt caagaatttg tctatggagt
     5761 gaatcatcat ttgatgggtg attttgtttc cttatccaat gaagaaattc gtcgctatac
     5821 caagcttgaa aaactaatgg aaagtaaatt ggctcgaaga tttaaagtca aacgagtaac
     5881 gccgagtgac ttgacgtatt taattgaaca tatctatggt gaaaaaggca caccgtttga
     5941 agaatatgaa ttccagctac caaagaaaaa gctgaaatca gaaacgctgg tgaaacgtta
     6001 cgacttatta cgtcccactc gttgtttgat tgaagaaaag cctcggtact tacggatgga
     6061 acatgaaaat catgagtctt atgtagcgta cttaacaatt aataccattg taggggaaat
     6121 ggagtttcct tcctcggaac ttttctatta ccagcaacaa caatttacgt ttccgattga
     6181 tacttcaatg aatgtggaaa ttgtgacgaa taaaaaggcc ctagctaccg ttcgtaataa
     6241 gaaaaaggaa ttaaaagatt tagacaacca tgcctatcaa tctgataatg aaacgaactc
     6301 caatgtatta gatgctttgg attccgtgga tgaattggaa accaccttgg atcagtcgaa
     6361 agaatccatg tataaactaa gctatgtggt tcgagtgagt gctaaatcag tggatgaatt
     6421 aaaacgccgc tgtgatgaag tgctcgattt ttatgacgat accaatgtaa aactagtccg
     6481 gccctttggc gatatgatgg gtctacacaa agaattctta ccctcaagta agcgatacat
     6541 gaatgactat attcaatatg ttacgtctga ctttttggcg ggacttggtt ttggggctac
     6601 tcaaatgcta ggagaattag aaggcattta ctttggctat aacgtagata cggggcgcaa
     6661 tgtttacctg aaaccggcat tggcatcaca aggggtcaaa ggctcagtaa ccaatgcttt
     6721 ggcatctgct ttccttggtt cgttaggtgg ggggaaatct tttagcaaca atctcttagt
     6781 ctattatgcc gtcctttttg gtgggcaagc tgtgattgtc gatccaaaag gagaaagagg
     6841 cggttggaaa gaaacgttgc cagaaattgc ggatgaaatt aatattctta acttgaccag
     6901 taagccagaa aatcaaggct tacttgatcc ctatgtgatt atgaagaaaa agaaagactc
     6961 agaaagtttg gcgattgata tcctgacctt tttaacaggg atttccagtc gggatgggga
     7021 gaagttccct gtgttacgca aagccattcg aacggttacc caaagtgatc agcgaggatt
     7081 attacaagta atcgttgaat tgcgtaaaga ggactcacca gtcgcggaaa atatcgcgga
     7141 tcacatcgag tcctttaccg attatgattt tgcccatttg cttttctcag atggttcgat
     7201 tacccagtcg attagtttag ataagcagtt aaatatcatt caagtagcgg atttggtttt
     7261 accagatgca gaaaccactt ttgaagaata taccaccatg gaattgctga gtgtttccat
     7321 gctaattgtt atttccactt ttgccttgga ctttatccat agcaaccgag aaattttcaa
     7381 gattgtggac ctggatgagg cttggagttt tcttcaagta gctcaaggaa aaacgttatc
     7441 gaataaatta gtgcgtgctg gtcgggccat gaatgccggt gtttacttcg tcacgcaaaa
     7501 cagtgatgac ttgttggatg aacgtttgaa gaataatatt gggctaaagt ttgcatttcg
     7561 cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggattag ataaagaaga
     7621 tgaaagtaat caaaaatgca tgcgagattt ggaaaatggg caatgcttga tgcaggattt
     7681 gtatggtcga gtaggtgtgg tccaagttca tccagtcttt gaagaacttt tccatgcttt
     7741 tgatacccgt ccgccagttc aagagagaat ggagtgagga gatgaaaaag aaactttttc
     7801 gtatctttgg aattgtttta atcagtttgg tcactctgat aatacttttg agcttagttg
     7861 gaacggtagc tgaggcaaca ggtctggttg atgatacagt tggagccgga aatctttatt
     7921 cccaatattc acttggtaac tatcagttgg atttctttgt ggatagttcg tgggattggc
     7981 tgccttggaa ttggggtgat ggcctaggga agagcgtaat gtatggactt tatgccatta
     8041 ctaattttat ttggacagtt agtctctact tatcaaatgc cacgggctac gtggttcaag
     8101 aagcctataa gctggatttc atttcagata cggctgaaag tattgggaag aatatccaaa
     8161 ccttagccgg cattaccgaa aatggactcc agacctcggg cttttacttt gggtttctat
     8221 tattgatgat tttagctcta ggagtttatg tggcgtatac aggattatta aaacgagaaa
     8281 ctaccaaagc agtccgggct gtcattaatt ttgtgatgat ttttctactt tcgggttcgt
     8341 tcattgcgta tgcgcccacc tatatcacaa aaatcaatga ttttagctca gatgtcagcg
     8401 aagcggctct tagccttgga accgagattg tcgtgccgaa ttcagaaagc caaggaaagg
     8461 acagtgtgga tttaatccga gatagcttgt tttccattca agtccaacaa ccgtggttac
     8521 tcttgcaatt tgatgattcc aatatagaag aagttggcga agatcgtgtt aacaaaatcc
     8581 tgtctgtaag tccagatgaa aataaaggca aggatcggga agaagcggtc aaagcggaga
     8641 ttgaagataa tgacaatgcg aatctcagta ttaccaagac catgaaccgc ttagggaccg
     8701 tggtcttttt ggtgttgttc aatattggca tatccttctt tgtctttctc ctaacaggga
     8761 ttatgttgtt ctcgcaaatt ctctttatta tctttgcgat gtttttgccg attagtttct
     8821 tattgtccat gttgccgact tatgagagtt tgggcaagaa ggcgattatt cgcttgttca
     8881 atacgattat gatgcgagca ggagttactt tggtgattac gactgcattt agcatctcca
     8941 cgatgttttt caatatttcg gcaacctatc catttttcat ggttgctttt ctgcaaattg
     9001 tgacctttgc cggtatctat ttcaaattgg gcgacattat gaatatgttc aatctccaga
     9061 gtaatgatag ccaatcaatg ggaagacggg tcatgcgaaa accgcaaatg ttaatgaatc
     9121 gaacattgcg gcaactcaat cgaaatgtgg gacggacttt ggcttttggt ggtggtgcag
     9181 cagttggaaa taagttggca aaggaacaat caaaatccaa atttaagcca tccggttctt
     9241 ctctacgaaa gaattcacga ttaccgaacg atcacgagat accatctgac ttgaataagg
     9301 aaaagccaat tccaaataat aagaaacaat cccgaatgaa tttggccggg agaaaagtcg
     9361 gcaaggttct ggatacccaa gctttggtga aagataagac caaacaagta aaggatggag
     9421 tacggaatac gccaaccaac ttgaaataca acttccataa aggaattgaa aaaacgaaaa
     9481 aagcaccgga agagtttaag cgaggactgg tccaagaaaa agcaaatcga ggagagttgc
     9541 gagaaaaaca acaacaacgt agagacgaaa aaatagctga aaagcgcaaa gtattgaacg
     9601 aaatagggaa tcctcatggg aaaagaaggg aaaatgttcc gataaaagca aaggtccaat
     9661 ctcaaaagga tagagcacca aagcggattg tgagagcaaa ttcgaatccc gaaatcaaac
     9721 gaaaattcgc tagccaagaa atcatttcaa aaggaataaa ccaatcctca agtaagaaaa
     9781 attcttttca acaagtgaaa cggcggtcta ctctgtccaa acgaaccaat cgaaaagtac
     9841 aaaaaagttt gaaccctcat cccaaaccaa ggtcaggtga gaaaaaatga acgtaaagaa
     9901 attaagtgcc atttcgttaa ctttcctaac aatctctttt atgggtatct tgatgtgtgt
     9961 gggcttattg tttggtgaag acagtgaagg aagtagtagt tcaggtgctc ctcctggggg
    10021 gagtagtgtc tcagaagagg ttttgaaaca ccgtgcaatg gtggaaaaat atgccaaaga
    10081 atcggatata acagagtatg ttccgattct tttagcgatt attcaagtgg aatctggtgg
    10141 cacaatggaa gatgtgatgc aaagttctga gtcagccggt ttaccaccga attcattaag
    10201 taccgaggca tccatcaaac aaggatgtct ctattttgcg tctttggtta agcgttccaa
    10261 agaattaggt tgtgatcaag atagtatcat tcaggcctat aactttggcg gtggttattt
    10321 ggactatgtg gcaaaaaacg ggaagaagca tagttttgct ttagctgaat ctttttcaaa
    10381 ggaaaaatct tgcggtcaaa aaatcgatta ccctaatccc atcgcgaata aggaaaatgg
    10441 tggatggcgc tataactatg gcaatatgtt ttattgctta ttggtgaagc aatacctaac
    10501 tacaacacag tttgacgata aaacagtcca agggattttt gacgaggcgt ttaagtatga
    10561 aggaacggct tatgtatttg gtgggtcaag acccgaaaca ggatttgatt gtagtggctt
    10621 gactcaatgg tgttatgcca aagttggtct gaaacttcct cgggtggctc aagatcagta
    10681 tgatgccatg acccatattg acctaaaaga tgccaaacca ggggatttgg ttttctttca
    10741 ttcgacttat gatgccggta cttatgtgac ccatgtcggt atttatgcag gggagaatcg
    10801 catgtttcat gcaggagatc cagtaggctg gacgaatcta acagaaagct attggcagca
    10861 gcatcttatc ggtgctggac ggtacaaata agagaaaaga ggaaaaagaa tgaagataaa
    10921 aattgaacgg aatcaaaagg aaaagagcct aaaaaagaaa aaagaacgag tcgtatccgt
    10981 gggtaagcat cgaaaaatgg tgcttgccct ttggctttta ttatcttgta gtcttacttt
    11041 tggcgtttat aaaaatttca ccgccattga tcaacataca actcatgaaa aagtggttat
    11101 taaagagaaa gtagtgaata cttctggtat tgaaaacttt acgaaggatt tttcgaaaga
    11161 atatttttcg tggaaaaata acaaagaagc aattgaaaag agaatgaaca acctcgaaca
    11221 gtaccttaca gaagaaggtt tcgctttaag tcaagatatg gttcgagcag atattcccac
    11281 taattccgaa gttcagtcag taaaagtctt agatgttgaa aagagctcgg aggaatttgt
    11341 cgtttcattt ttagtggagc aaaagattac agagggaaaa aaggcacaat cgatttcttc
    11401 tgcataccga gtgacgatct ttgaagatga aaacaggaat tatatagtaa cgagtttgcc
    11461 aactatgatt gcaaaacctg atagggcaaa atacaagaca aaacaagtag aaaatgattc
    11521 gaaaattgat gccaaaacta cagaagaaat cgctgagttt ttggaaacgt tctttaaatt
    11581 atatccatca gcttctgaaa aagaattgga gtattatgtt gaaaatgatg cgatgcgacc
    11641 aatcaatatt gacttaaagt tttcgaagtt agaggatcca gtttacctac aaaacggaaa
    11701 aaaaattcta gtgagtacaa agataaaata ccttgataat gtatcaaaaa ccactagcta
    11761 ctttcagtac aaattaatat tacaaaaaaa tggaaactgg aaaatcattg atgttaaata
    11821 ataatgactc aatctaaatt tatgagaagt tataagtagt ttaacttatt tcaggcagaa
    11881 gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg taactcgaaa accgacaatt
    11941 aagacatagt gcctttttat tttaatccat gtcataatca gtctatataa gaaatggttg
    12001 atataccatg gaattagata caaataatca ttcattattt cttctttgtt accaccttat
    12061 tttagtagtg aaacattgta aacatctcat tgatgatgta atgttagctt atgcaaaaaa
    12121 ctatctttga aagaatatca gaatcacatc acattacctt agttgagtga aatcgtgata
    12181 aagactactt tcatattatg ctcaaagcac aacctaaaac cgaattaaca aaattcagca
    12241 atgcctaagc tcatgtctga taaaatgaga ctttctaagg gtcaaaaatt tcctttgaat
    12301 acgatcatta gcttagataa aagtaaaaac cttagaacta cggggatagc ttggttaata
    12361 agagatatcg ctgttaataa agacataagc ttgcaagtat gctctattcc caagaatctc
    12421 ccacttcaag cactaaggat cgttaaattc agctaagtgg caagtagttc actagacgat
    12481 aattatctcc atatttcaat ctcagctttt ccatttttca tagcaaattc cgttattttt
    12541 atagcatcta aaagattcag cttttccact gatctttcac cacttttcag cttttttatt
    12601 gtgcttaaat tgataccagt accttttgat attgaattgg aagtaaaatc attaagggtt
    12661 ttttttatta aattcatatc cgcaattcca gcgttcataa gaatgaagat tctcctctca
    12721 tagactactt tgaaaatggt actacagtgg ctgtttatat atttcagtag gttactgtag
    12781 tggtcatttt attataatta gaaaaggaac ctaaatcaaa aatgctaaag aaaattttca
    12841 tagtattaaa cctatcatga ctaaagccac ggaattcttg ataaaagagt atacttgcaa
    12901 gcctatgtct ttactaacag cgatatctct tattaaccaa tctatccccg ttgttcctaa
    12961 ggttcttgat gttttctaag gcaatgcttg tatacatagc ccttgatcaa aatattgata
    13021 ctaacgttga cgtttcgatc atgatgtgta cggcaaatag gacaagtcca ttctcgaatt
    13081 gtgaatgact tcttaccatc tgtgtgtcca cattctgaac caatttgact agacggaaat
    13141 catttatcta tcttgatgat ttttttgaca gaagtatcga aaatctttac ataatctggg
    13201 tgttgcattc ttatgcagat gagagcgtta gctccgtatt cataaccagt tgcctctaaa
    13261 acgtagtcca tcggtggata cctagcttgc ggttgccagt ggctttcgca gtcttcttgt
    13321 aatcaaatcg agcatctaat tgagtactag tattttgaag ataatacttg agtttttcgg
    13381 ttttcagaag ttcacttcat cttccatcat ccatggaaat cccactcacc catacgcagg
    13441 cctccaaaaa ttcttcaaca atggtttttc agacattttt ctgaatacct ccttgttttt
    13501 tgatacgcca gatagtcagg aaaaaggcaa caaaacacaa aacaaagctg cctaaaaagg
    13561 taatcttcat tccatataaa aagacatccg gccgatctgc gatatagtta gttactcgtt
    13621 ggcccatctt agcagacatc gcttgatata agaccgtcgt agaaagcgaa atcccgatga
    13681 ccatccccaa atttcgggca aaggaattca tggagcctgc cagtcccaaa ttttcgatag
    13741 tgacagaact cataaccatc gtattatttg gggactggaa caaggcattg ccggcaccca
    13801 tgatggcagt agcgacaatg tacagccaca aaggagagtc cactgacaaa aacatataca
    13861 tgacttgggt taccgacaat aaacccaagc ctactaatac aaaaatctcc gggccaaccc
    13921 ggtcggtcag gtatccgctt aaaggcgccc cgacgaccat catcaaggga aagaccatca
    13981 tcaacagtcc ggcatagcta ggactcaacc cgcgagcatt ttgcagataa aaaggaatca
    14041 ctacattaac aaagaagttg gaagaaaaaa tcaacaaggc tgtaatcaag ctcatggtaa
    14101 aaatcttgtt cttaaaaata ccgaaagaaa tcaatggaga ggctgctttc ttttcaactt
    14161 ttaaaaagct gacaaaggca atcactgaaa ttacgaacag tacaaggggg actggatgga
    14221 aaaagccact ttcttgcccc aaaaagattg ttccaaaaag gctgataatc gtagccgcaa
    14281 tggcagcaaa gccccctcca tcgattgtat ggccgctcat ggtgatatct tttggtaaga
    14341 atttttcagc aatcaacatc gttacgattc ccaccggtac attgatccaa aaaatgtacg
    14401 cccaagaaaa ttgggacaaa atcaagccgc caagacctgg tccagcaatc gagcctaatg
    14461 aaacaagcgc accgattgct cccaatgccc gaccacgttc atgtatggga aagacttccg
    14521 tgatgattcc ggcatttgta gccatagtca tactagcacc aatggcttgt accacccgtg
    14581 cgaacaatta aatacctagc gaatgattga acccacacaa cagcgagccc aagacaaaga
    14641 tcagcgtacc aatccgaaat actttgattt tcccacagct atcgccaatt ttaccaaaca
    14701 gcaacaaaca agaacagaca atcatcagat aaacggacac tacccattcc gcttgattca
    14761 ttggtatgcc catatcatct gagatggtag gtagtgcaat attgacaata ctgccatcca
    14821 atgtggacat gaaggtaaac atagatatag caatcaaaat ccaccagcga tttttttgga
    14881 cttttgcatc ttcttgataa ctatgcataa taggatttcc tttctaatga tcgtgtagct
    14941 aagaacaata taacctatgt ctactgccca acaacttctc gcgactctag ccataggaag
    15001 cttgagtaat ttcgatttgt tattctcatg taaccatgta agtttgagat gctttatttg
    15061 aacttactct atagtaacta gtgataattc gctttacttt cttctagcaa tttctaaaag
    15121 tcgacttcat tgttcttcag attaatagtg tgagctatct cgtgagacgt aagaaaattt
    15181 ccattctact ttttcccaat tttttcatat ttatggtgtt gcacctattt gttttccaat
    15241 ttgcttttca aatacattgc tatgatattc aattccatag gattatacta aatgtcaata
    15301 gtatgaataa tatattatag atgtttatat atcactgtta attattatcc acaataatta
    15361 atgattacct cgatatttgt caatcaaagg ttgaaacaat ttattttcag catcttttct
    15421 aacttgagct gcactcttaa tagaatcata cgatcctaaa tagtacctct tttttttaaa
    15481 gttatatacg caatccattt ctctttagaa cgataaactc ccttaacacc acttgtatta
    15541 tttttcggta attttgacgt tagacactct attactgtcc cttcaacaca tgtccttgtt
    15601 tcttgccttt ttaaacatcc acaactttga gtatgtccaa aaaataagtt actataactt
    15661 atctcacaca tactgccaca ctcacattga caaacccatt ttgttttcca tgaacagtta
    15721 ttgtccaaag gatactttgc agtaagtaat ccgaattttt tattttcaat gtgatgcttt
    15781 tttgatttca aacatccaca gctttttaca tcacctttta gaagtcgctt tgccgatact
    15841 aacttttttc tcccacacag acattgacat tcccaaacaa cagttccttc acttcgcagt
    15901 ttagttggct tgattgcaac taacttacca aaagttttat ttgtcaaatt tttatattta
    15961 tgcctatttt tataatttcc acatccacaa cttttggttc tacctgatag taagtttgat
    16021 cgatatacaa tcgtttcatt cccacagtca cataggtaac gatatttttt atatccatta
    16081 tgagtaatct cctcaacaac agtaaggtga ccatatcttt ccttcactgc acctaccctc
    16141 ccaagaaaga agtaccatat aatttcaatt aacttttaat aaatcagaaa aaaatatttt
    16201 caatttttct tagtctgatt ttatgactaa tttataatac aagactgaac gatacgtaca
    16261 cgaaatcctc aagatagata aattgttttc atctagtaaa acctgctcaa aatatgagca
    16321 taaagacggc aataatacac tcgatattcg ataatggact tgttttattt gtcaaactca
    16381 tcgtaatcga gatatcaatt ccagtctcaa tatcctgatc aaaggtctaa gaatccaacc
    16441 attttcttgt tactttgtca gattgattac cttttttagt ctccgaataa taaggttttc
    16501 ggatcaacta acatgctctg agaaagacta ctatctttgc aaatttccaa atgaaggtgt
    16561 gggccagtac tattcccagt acttcctaca tagccaatta tttgtccttg caaaacatta
    16621 tctccagttt ttactaggta ttgttgctgg tgagcatata acgcggttga tccatcttcg
    16681 tgttgtatca ccacgtaatt tccccaagag tagtgaaact ctgctttaac aaccttacca
    16741 gctttaactg catgaatcgg ctcaccctgt gtagctgcca tatcaagtcc tcggtgaaat
    16801 tcatttccac gatttccaaa caaactggtt attgaataat tatgaatagg cacttggtat
    16861 ccagtcttac tcatttgtgg acctttaact tctttatcat agttggttaa atcataggtt
    16921 ttaattaatt catttagctt caaatagtat tgtgtatctg ttgcataccg tcctgtaagg
    16981 aatttagttg cttctcgata tgttttacta ttagctttta atgctcctgc ataaaaatct
    17041 ttatttccac taattccctt agttaataat ttggcataat cttcaaaact ttcctcatag
    17101 ttttcataca ctctaaattt tgattgggtc gtataaagcg ttccattacc caaatcttcg
    17161 tttgttacca taatgacaaa gtttccatta taagttcctt ttatcccaaa taaattgtaa
    17221 ttcggtgcct gagctaactg gctttgacca ctggcagatt ctaagatcgc ttgtgcaatc
    17281 ataacagaag catataaatc atatttctga ccaatttttc tcgcagactc accaattttc
    17341 ataataaaat tttctacaga ttcatttttg tcaaaatgaa tttcttcatt accagatagt
    17401 attggttgtg attggaaaga ttctgttgat tctatttgct gtggtgtcga aaaatttttt
    17461 gtttgtcctt ccgtttttgg cgcttccttt tttgaagact ctatatggtt tggtcttgtc
    17521 actttcgaag aagttatttc acttgtacta cttgaagttg ttgaagattc ggcacttgaa
    17581 atcgtactac tttcacttga cgaagttgtt gaagactcgg catttgaagt cgtactactt
    17641 tcacttgacg aagttgttga agactcggca tttgaagtcg tactactttc acttgatgaa
    17701 gttgttgaag actcagcatt tgaagtcgta ctactttcac ttgataaagt tgtcaaaagt
    17761 tcactactct ctgctgttac atcggaatta ctcatttcag tcgtctcggt tctttctttt
    17821 gcaattttgt atgttgaatt ttcgcttgcc aacacatgga aaggtgttgc tataaagagt
    17881 tgggacatta atataactgc tgatgcaagt tttaaagatt ttttcatttg atttacctct
    17941 cttttttctc tttaatagtg ttttctatta aaaattatga taaatatttt caatgaattg
    18001 agtaatacag tatatttctt gatttcattt agtaacgatt atgtttcaat gtttaatcct
    18061 gttactgcct ttaaaataaa attatagggt tggctaaaac taggatgaaa taaataatct
    18121 aacattttca atttgtctaa tgtaatttgt tcttctatag caagagaaaa cattgataat
    18181 agtccagata aatcttcttg actacaaagt tgtacaccta ctattctctt tgtatttttt
    18241 tcaaagctaa ttcttaattt tacacgacta ttttttctca aaaaaacagg tctaatgaaa
    18301 tcttctattt ctatataatc tatagactca cttaataatc gtgctgtttt taaattcaaa
    18361 cctgctgcgt aaaaattgta attaaatata gaaaagccat ttgtaaattg ggaaccagaa
    18421 ggtgtaaaat cttgattgtc aatatgttga gttgcaatat aagctgttct tagagcatta
    18481 gaaatagaaa aatcaacact acttctatta atggacgtaa aaaaagttgt agcacaatcc
    18541 ccaatagcat atatatcttt tatatttgtt tgctggtatt cattagttag atatgctcca
    18601 ctaacatctc tcaagagttc ctttttgcca attggactat tagatagaaa tcctatagct
    18661 tgtacgacca tatccataga gtattgggta ccacttgtag tgactatttt ttcaatcttt
    18721 tttcttccta aatatttttt aggactttct gatagacaaa gatgtattcc attttttgat
    18781 agtatttctt caactttatc tgaaaaacta cggtcgtaat aagtcgacaa cactctatct
    18841 gcaacatcga aaagatatac ttctttccct ctttttctta tggcttcagc aatttcaact
    18901 ccaatatatc ctgctccaat aattcctact tttttaatag tattatcatc taaagcttta
    18961 aaaatctctt tagcatttgg aaaattcttt atcgaaaaaa tattagctaa attattcccc
    19021 ggtacatcta acataatttg ctttgaacct gtagctagta taagtttgtc atattcttgg
    19081 gaaaatcttg tgccatcttt ttttacacaa ataatctttt tattttgaat atcaattttg
    19141 tcaactgctg tctccaaaaa caactttttt atcttagaat ttagttctct tctattttga
    19201 taaaaaagat cttctaaatt gctaacttct ctttttatga acaacgatgt cccacattct
    19261 aagtaactaa tttctgattt tttatctatc agaataactt cataatttga atcacataag
    19321 tttagaaatc gagctgcagc tagccccgaa tggtttgatc caataattac tatttttttc
    19381 acttaatcac attcttttcc cttctatgaa aagtatcttc atttttaact aacaacctat
    19441 ctcccaaaat aaaaaacaaa aagaggaaga aaaagattct tcctcttttc agagaagatt
    19501 taagaaattg attttctatt cattctcttt ttttcgattt ttatacccta ataaccctaa
    19561 agttcctaac aggattaaac caccactaat agtaaagata ttactatcaa tctctcccgt
    19621 cttaggataa gcttttgagg actgcttttg aggactgctt ttgcctctat ttttgttttg
    19681 cttgctacct tgattgtcag tcttattatc tttactaacg gctttttcac tgtctttttc
    19741 gtttgaagaa acaactacat tagtaggttc actagtatta ccagattcat cagttgcagt
    19801 tactatcact tttgttcctg cctctaattt aggaacttta actgtaaaat taccattttt
    19861 atcagctgtc ccatggtatt gactcccgtt tggtaatgta actgtcacct ctgcatttgg
    19921 ttcagtttta ccagtaacaa tttggtcacc ctctgtaata ggatcaactt gaggtttatt
    19981 cggtgctttt ttatcttcta cagaaatagt aacctctact tcttctttag atccatctgg
    20041 atatgttact actacaatcc cttttttgtt tccagctgta ctaacatcta ctttctcttt
    20101 ccaagtataa gtagttcctt ttggtagatc ttctttgttc ttaattccct cagatgcgtc
    20161 tggctcttta tttaagtctg tagtcacttt ttgtccttca ggattgtatt tatcggcatc
    20221 ggaacgattg tctgtcactt ctactggaac tttcactgta tctttcgtac catctg
//
LOCUS       DACDNO010000053        20249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDNO010000053 DACDNO010000000
VERSION     DACDNO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.053"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            194..2236
                     /locus_tag="IWI22_002022"
     CDS             194..2236
                     /locus_tag="IWI22_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9169404.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2247..2867
                     /locus_tag="IWI22_002023"
     CDS             2247..2867
                     /locus_tag="IWI22_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9169405.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2879..3337
                     /locus_tag="IWI22_002024"
     CDS             2879..3337
                     /locus_tag="IWI22_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9169406.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3356..3634
                     /locus_tag="IWI22_002025"
     CDS             3356..3634
                     /locus_tag="IWI22_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9169407.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3659..5026
                     /locus_tag="IWI22_002026"
     CDS             3659..5026
                     /locus_tag="IWI22_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9169408.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5041..6186
                     /gene="dgoD"
                     /locus_tag="IWI22_002027"
     CDS             5041..6186
                     /gene="dgoD"
                     /locus_tag="IWI22_002027"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP9169409.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6213..6704
                     /locus_tag="IWI22_002028"
     CDS             6213..6704
                     /locus_tag="IWI22_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169410.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7147..8346
                     /locus_tag="IWI22_002029"
     CDS             7147..8346
                     /locus_tag="IWI22_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9169411.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8841..9599)
                     /locus_tag="IWI22_002030"
     CDS             complement(8841..9599)
                     /locus_tag="IWI22_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9169412.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9767..10702
                     /gene="pfkB"
                     /locus_tag="IWI22_002031"
     CDS             9767..10702
                     /gene="pfkB"
                     /locus_tag="IWI22_002031"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9169413.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10699..12144
                     /locus_tag="IWI22_002032"
     CDS             10699..12144
                     /locus_tag="IWI22_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9169414.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12172..12627
                     /locus_tag="IWI22_002033"
     CDS             12172..12627
                     /locus_tag="IWI22_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9169415.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12646..13620
                     /gene="lacD"
                     /locus_tag="IWI22_002034"
     CDS             12646..13620
                     /gene="lacD"
                     /locus_tag="IWI22_002034"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9169416.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13731..14292)
                     /locus_tag="IWI22_002035"
                     /pseudo
     CDS             complement(13731..14292)
                     /locus_tag="IWI22_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14436..15050
                     /locus_tag="IWI22_002036"
     CDS             14436..15050
                     /locus_tag="IWI22_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9169417.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15291..15674
                     /locus_tag="IWI22_002037"
     CDS             15291..15674
                     /locus_tag="IWI22_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9169418.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16037..16546
                     /locus_tag="IWI22_002038"
     CDS             16037..16546
                     /locus_tag="IWI22_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9169419.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16646..17296
                     /locus_tag="IWI22_002039"
     CDS             16646..17296
                     /locus_tag="IWI22_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169420.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17747..18685
                     /locus_tag="IWI22_002040"
     CDS             17747..18685
                     /locus_tag="IWI22_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9169421.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18698..19750
                     /locus_tag="IWI22_002041"
     CDS             18698..19750
                     /locus_tag="IWI22_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9169422.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19808..>20249
                     /locus_tag="IWI22_002042"
     CDS             19808..>20249
                     /locus_tag="IWI22_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9169423.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
BASE COUNT         6416 a         3217 c         4277 g         6339 t
ORIGIN      
        1 ccgaatattt aatattcaca ttagccctaa aataattgtc ataccatgtt acatttgata
       61 taacagcgtt cattttaaaa atagctgtta gtgtgacaaa acattaaacc aagtgtgaca
      121 aaaaacgtga tgaaagcttt ccggaaacgt atgtgaacaa gtatataatg attttgtcag
      181 gaggatagtc aaaatgaata aacgaattcg tgaaatactc aaactgatta ttaaaaaccc
      241 tgaaatgaaa ctttccactt taacaagtga acttgatttg acacgtcgtc aaatcaatta
      301 cgcgatcaat caatttaatg aagaattgaa aacaaagaac attccgttaa ttcagagaag
      361 tcattctgga aatattacgg taccaatcga ggttatccaa atgatatcac agcctgatta
      421 cgacataggt gatgatcagg aacttgtcgt tctcactgat agtgaaagac aagcattgat
      481 agtgatgact ctgatcgcta atatcgatta catcagtctg gatcatttgg tagatgcggt
      541 tgatgttagt aagacgactg ttatggaaga tatcaaacga acggaaccgt tcatcagaaa
      601 ttatagtctg acaattcgat ataatcggat gaatggttac caattggttg gttcggaaca
      661 ccgtatctta cagctgttat cggatctagt aaaacggttt ccgatttttc gccgagaaat
      721 gattcgcaga aaattggcac aagatgtttc tgaagaagaa atcgtccatt tgatccataa
      781 catggaacaa atgttgcatc ttagttattc tgatgagtcg gtagactatt tgcagacatc
      841 agcgagattt ttagtaagcc gtgggatcaa gatgtcagat aagcacgcgt tttttcccga
      901 tgatgtgacg gatactcctg aattccgaat gctgatgatc ttggtacatg aaacaggttg
      961 gagtctatcc aaaagttata tggagtggtt cacgttatta tttttaactt ccaatatttt
     1021 tgagagcaaa actactcaaa attatgattc ggatcaagaa ttgcgaaaat tgatcagcca
     1081 aatggttgaa aatttccaaa atcaaacgtt gattttgatc gatgaccgag aagactttga
     1141 acgtaggata ctgagtcatc tgcgtccagc ctgttttcga atacgctata acttgagttt
     1201 aggagtttat tcaatagata gtctgatcaa ggatagtaat catgcgattt tgattgattt
     1261 gatgaaagaa ttgattattc ctattgagaa ttggctaaat aaggcctttc caaatgatga
     1321 attagatctt ttatcctatt actttggcta tcaattaagt aatcataata aattgaataa
     1381 gcaacgacaa agggctgtag ttgtttgtac taatggtgtc atggtctcaa agttagtacg
     1441 agccaatatg gaaaaacttt ttccagagat ccatttttta gcttccctat ctgtgcgtga
     1501 tttctacaaa tttgaagctg attatgatct ggtctttact acgacgccac taaaaagttc
     1561 tatgctgcag tttatgatcg agccaatcat gacatatcag caacaaatca gtttacgata
     1621 tcgagttttg agtgatttag gaattactaa ggttgatcga gcaatggatg aattgttaac
     1681 aatcattagg aaatatgcaa atatcaccaa tccagtttcg ttgcgagaag aactgcagta
     1741 ctttttgatc aaggaagatg gaagcacgcc gctggacaac ttcaatgtat tgccttcatt
     1801 gacttattac ttgcggccga gttatgtaca aatcgttgaa gaagagatgt cttgggaacg
     1861 agcagtggag gtggcttgcc gaccgttatt ggaacatcaa atcattgact cgcgttttat
     1921 cgctgattgc ttggatcaaa tcagacaacc gggatatgca ggttacttgg gaatgaagac
     1981 ttgcattccc catacaacgg tcgataaagg tgtaatcagt gacggtgtca gcattttagt
     2041 ttgtaaaaag ccgatacgat ttcctaatgg tgaagatgtc agcttgattt tgccgctttc
     2101 attttttgat ttaacgaagc atctgcgagc catcaatcaa atcgctgata tcgccaaaga
     2161 tgattcattg attgagcagt tattgattgc tgatgacgaa aagacagttt atcaactatt
     2221 aagacaaaga tcttaaggag gaaaacatga gactagcaga ttatcctaaa ttgacagtga
     2281 tcatgcgtgg ttatactttt gatcaggcag atgctatttt acaagcaatg caggaatttg
     2341 accatcaatt tgccgtagag atgacaatga acacaaaagg agctcttgaa aatatacatg
     2401 aactaaatca acgttatggt gacacgactt atatcggcgc tgggacggta aggacattag
     2461 aacaagccca agcctgctat gaggctggtg caaaattttt attaggacca catatgttca
     2521 caaaagaaat gttgtcttac gccaataaga aaggattatt ggcggtacct gccgcaatga
     2581 ctccttcaga agtcgaccgt atgtttaaag aaggagctga tattgtaaaa gtatttcctg
     2641 cagcggttgt tactccacgt tttttcaagg atattcaagc acctttaggt aagttaccac
     2701 taatgggggt tggtggaatc agtaaggaga atgcgaaaga gttttttgaa caaggtgcta
     2761 gctatttagg tcttggttcc ggtatgttca acaagcaaga catcgaagag ctaaatgtta
     2821 aaaacttagc acgttcgatg aaagaattaa ttgaggctat taaataggag tgaggacttt
     2881 ggaactttat ttgaatgagg aactcgtttt tcgaaatgtt caagcaaaaa cagatagtga
     2941 agtcttagcg tttttagcaa gcgagatgta caaaaaaaac tacgtgaagg aggaatatat
     3001 tcaagcaatc caagaacggg aaaaagaata ccctactgga ttaccatcaa caccaccagg
     3061 aatcgcaatt ccccatgcaa actatgaaat ggtcaataaa acaactttag caattgcgac
     3121 tcttaaagat ccggtcctat ttcataatat ggaagataac aatgagcagt taccaatcca
     3181 aattgtgatc atgatggcga ttggggaacc acatgggcaa gttgaaatgt tacaaaaaat
     3241 tgttggaatc atacaagatg agccactgcg gcaagaaatg atcagagcag gcaatgatac
     3301 tgaattatta gaactattaa agaaagcggt tttttaatat aaggaggaaa tcataatgaa
     3361 acgaatttta gtagcatgcg gaaatggtat cgccacttca acagtagtag caacaaaagt
     3421 tagagagtat ttgcaacaac atggcattga tgtttcaacg actcaaacaa aattgatgga
     3481 agttccagga aaagttcaag actatgatct attggtgacg actggtcaat ttgatggtca
     3541 aacaggtggt gtaccagtaa tcaaagggat gccgatttta acaggtatcg gtgccgatca
     3601 gacaatggaa gagattttga acttactgaa ataatagctg atcaaaggag ctgaaaaaat
     3661 gaaaatcatt atgaacgtct ttaacttctt tattgatgct ggaccaacag ttatgttgcc
     3721 agtcatcatc acgattattg gtttgatttt tgggttaaag atttctcgtg cttttaaatc
     3781 aggtttgaca ttaggtatcg gttttgccgg gatcaagttg atattggact ttatgacgac
     3841 caatgttgga ccagctgcca aggcgatggt tgatcgaact ggtgttaaac tagatgcttt
     3901 ggatgttggc tggggttcga ttgcggctgt tacttgggca tcgccaatca ttccaatctt
     3961 gatttttgcc attctattgg tcaatatcgt tttgctgatt ttgaaacgta cacatacgct
     4021 ggatgttgat atctggaact atcaccatat ggcaatcgtt ggtgttatag tgtattttgt
     4081 gactaagaat gtcttcttag gtgtaggtgc atcagtagtg atggcaattg cgacttttaa
     4141 gatttcagac tggtcgcaac cgatggtaga aagtttcttc ggaatccctg gtgtttcttt
     4201 accaactgtt tctgcgcttt catcactggt tattgcttgg ccgttaaatt ggctgctgga
     4261 tcgtatcccg ttattcagaa aatcaaaatt caccatcaaa gatgcacaaa aatatttggg
     4321 attttttggt gattctatga tcatggggtt agttattggg atggtgattg gtgttttagc
     4381 cggctatgat atcaaagcgg ttttacaatt aggtgtcagc atgtcagctg ttttggtttt
     4441 gattcctaag atgacttcat tatttatgga aggattgatg ccaatttcag atgctgcaca
     4501 aaaatggtca caaaaaaaat tcaaaggtac acgtttattt atcggattgg atgctgcagt
     4561 tgttgtaggt aatccagatg ttatcacaac agcattgatc attattccac taacgattgc
     4621 attagcttta gtattgccag gaaaccgtgt gttaccattc gcagatttag cggttgttcc
     4681 tttccgcgtg gcaatggtcg ttgccttgac tcgtggtaat ttattaaaaa atatcattat
     4741 tggcttagtt gtgacagctt cattgctatg gtgtgggtca gctactgcgc caatgctgac
     4801 agcaattgcc aaatcggttg gtattaactt aggaacttct tcaatgttaa tctcatcatt
     4861 tgctgcgact tccatgatcc aaagttattt ggtatttatc gcatttgctt acaaaccaat
     4921 catcggtatt ccagtgttgt tattagcctt tgcgggcgca tggtattact tcgatggtat
     4981 caaaaaagtt aatcatgaaa tggatgaacc attgaaggca caatagtagg agggtttaaa
     5041 ttgaaaattt cagatgtcac agtgtataaa gtaaaaccaa gatggatttt tgttaagatc
     5101 tcgactgacg aaggaatcga aggctgggga gaaatgatct caggaactaa aacagaaacg
     5161 gttgttgctg gagcttatga gataggaaac cggctgattg gacgtaatcc gtttgaaatc
     5221 gagcgcctct tccaagaaat gcatcgatct ttcttccgag gcggtcctat taatggaacc
     5281 atcgtttcgg gtttagaaat ggctctgtgg gatatcaaag gaaaagcgtt taatgttccg
     5341 gtttatgaat tattgggcgg tgcagcacgt gataaaatca aggtttattc ttggattggt
     5401 ggagatcgac caagtgaagt tgcagaacaa gcacaagatc ggtttgatcg gggctttacg
     5461 gccgtcaaga tgaacgcgac agaagagctg cattatatcg attcctacca aaaagttgat
     5521 gaagtggtgg aacgagttgc gtctattcgc gaacgttttg gtgatgcaat ggatattggt
     5581 gttgattttc atggacgggt ccataaaccg atggcaaaag tgctagcaaa agctttggag
     5641 ccattccatc caatgttttt ggaagaagtc gttttacctg aaaatgaaga acactttaaa
     5701 gaagtagctg attctgtggc tgtaccatta gctactggtg aacgcttgta cactcgttgg
     5761 caattcaaga acctctttaa acaaggtact gttgatatta tccaaccaga tgtggctctt
     5821 tgtgggggaa tcttggaaac ccgtaaaatc gcagcgatgg cggaagctta cgacatggct
     5881 gttgcgcctc atgcgccata tggtcctgtt gcactagcag caactctgca agtagactca
     5941 tgtacgccaa acgtctttat ccaagaacaa agcttaggga tccactataa caaaggtttt
     6001 gatctgttag attttgtgaa gaacaaagaa gttttccaat ataaagacgg ctacgtagat
     6061 ctaccttcaa aaccaggtct gggcttggaa atggatgaag acaaaatcaa agaaattgca
     6121 caagaaggtt tgatttggac caatccgcaa tggaaaaatt atgacggtac gatcgctgag
     6181 tggtgataaa tgaaaataaa ggatgatgaa agatggcaga agcacttatg atcacagttt
     6241 ttttaggact gctgattatt ttgcaaataa tcgatcaaaa gaaaattatc gtgtttgctc
     6301 agagtagtcc tgtaaggaag agtttgagta ttttactgac agtcgccatc ctgttgattt
     6361 tctggccgga acatctgact gatcagatta aactggttgc ttttgcttct gtcattttga
     6421 tcaatggttt tttgaaggag ggtctagcaa aagattctgt tgttagattt ggagtattga
     6481 acggtaactt taatcgatat cctcaagtcc aagtagaaaa actaggtgac gataaatcat
     6541 ttatttcatt ttacaaaagc aagaataatt ctttttcaat gatttttgat aaagatcaaa
     6601 ctgaaatgat ggatgtcttt caagaacttg atatccattc caaaattact ttagatcaaa
     6661 gttcggaaga aaaaagagca gttgtaaaaa aagataatga atgattctac taccgaaatt
     6721 atgatttctt tatcacgaat aatgaaactt gttttaatga atcatattga ttgaagtata
     6781 caatgtatat taagttttgg aaagaaatat attggtacaa gatatagcag gaatcgatta
     6841 ctcgatccct gcttttttta acttcctttg cagcaaaaaa agctcgtaat ctaagagatg
     6901 atggatagtt tttagaatat atatattttc ttcaattcca gaaaaaaatg accaagctag
     6961 tatgctaaaa aagtacgatg cttctatgat cttttttccg tctttattaa cagatgtttt
     7021 aattgatcaa cgcatattgt aatctaccct aatttccata gttcttcacc acttttaaaa
     7081 atagtaaaac ccctacaagt atgctatagt aaattgctaa agaaaaacca ataaggtggg
     7141 agatgtgtgc gcgatcaaac aagcggaaaa atgattcgtt tttattatac aagtttgcat
     7201 tttctttatt ggatgatgtt ttgttctgtc tatgggtttg cgacattgtt tttgatttat
     7261 ttcaagattg atccagctaa aatcggaggg atcttagcat tggtcaatat tgtttcaacg
     7321 gtgcttcagc cttttgtcag tcaagtgatc atcaagaaaa gaaagatccc actgatcacc
     7381 gtgctgaaga cgatgatagc aggattagga gtgattctct tagctggggt attgattccg
     7441 aatttgccga tcattactta tatcttgggt gggattttag tagtttctat gcaatcattt
     7501 ctcaatgcgc tggcatttga gtattcaaat accggctaca ggatcaactt tggcttttca
     7561 agagctggtg gttcattttc ttatgctgta acttcttttt tactgggaca gctgttagcg
     7621 atttattctg ccgcgatttt gccaatcttg gttatagttg aagcagtgat atttttcgtt
     7681 attttgcatc tgctgccgga tgtaaaaaat caagaagttt ttcctatgac aaaagaacag
     7741 gatcgctcag tttctttgag aaaaaaatat cctttcctga ttttgttatt tttaggtttc
     7801 agttttcttt ttactttcca caccatgatc aacagctttt tggcacagat ctttgaaaat
     7861 atccacggcg gcagtaaaga agttggcatc gcattgatga tcgccgcttt atgcgaatta
     7921 ccgggaatga tcttttttga acagcttgtc aaaaagaaat ccagcaaatt ttggctgtct
     7981 gttgccagta tttcatttgc tgtcagagga ttcatgatgc tgctggccag ttcgatcctg
     8041 atgatggaaa tgacgcatct cctacaagcc ttcagtttcg ctttgtttat tccagcttcg
     8101 gcttatttgt tcaatcaatt tctggcggat gatgatcgtg tatttggaca aacgatgatc
     8161 atcatcgcca caactcttgg tggggtgata ggaaatgtct tgggcggcag tctgctgcag
     8221 catttcggtg tatgggagat gctgattttc ggtactggtt ttgcggtgct aggtgcttta
     8281 ttaactattt tgggcattcg aaaaataccg aagacaggaa acgatagtga ttctcttgtt
     8341 gagtagcata aaaatactta gatactagtt ttatataaat aacatatagt gattattggc
     8401 gtaattacaa aaaacgagaa taacttaagt aagcatttct aaataattga gaaacctcat
     8461 ctaaaaagat aagaaaatat ccctaataaa acttctgtaa cattttagat aactgattgt
     8521 aatctgaata ataaactata aggagacggt tagacggtct cctttttttg cgtattataa
     8581 ttctagaatt gttggaaatt gataataatt aacaagaaat attagacagg aacttcgaac
     8641 tgcttatcac attcttatta ttatatattt gcgtaagtag tcctggtagt ctcaatgcac
     8701 tattctatct tgaaactaag aagttataga aggagcaatg atagaaagca cgagaataca
     8761 aatagcgatt aaatattttt ctagacaacc ttagttaatt cacacagata tattttaggt
     8821 acagtcttac agatatagag ttagtgctgt gtgaatcctg atgttcgcag tgcataggag
     8881 tcaagtaaga ttctagccag tatagaaagt ggaagtctgg agcgtacctc ataatctggg
     8941 aagaaggagc cttctgattt aaagccaaca aaaggaatct cgatatgttc catcaaactt
     9001 gaatgacgat tagcagtaag caaaattgtc ccgatctcag ctttataaca gttttcagct
     9061 gcggtagtca attcctttgt ttcaccgttt agtgaaacaa agatcacgat gtcatctttg
     9121 gataattttc tactaatgtt ttttatgata ttgggatcag tatgcatctc acaatattta
     9181 ccggtaagtt gaaatttgac catcatttcc tgagcaatca attctgaaaa acctcgggca
     9241 aaaatcatga ttcttctagc agccttgatt cgttgaatag catcttcaat attcccgctt
     9301 tctaccatat tgatggtgcg gattacctct tgctcatttt taagaatagc agtctttatt
     9361 ccttcatcaa ctttatcaag agcggcaaaa ttgatatttg tattttcttt ttcttttaac
     9421 gaatgtttaa aagacgtaaa cccctcatat ccttttttct tcatagtacg aacgacggtt
     9481 gctgttgaaa cgttggcata ttcacttagt tttacaatgg aataggaggg gatttcgagt
     9541 atgtgacttt gaataaaatc ccagagatat ttctccgatt cacttaattt tgtatccata
     9601 ttttgcctcc gattagttag gataattcta ttgtaaagcg ggagtttcct ttacttcaag
     9661 tttatttaat atattttgta aaagttttca atgaatttag tcatttatga aaacatttac
     9721 attcgatctt gatacataat gaatatgtaa gaaaggagga gcgtaaatgt cagtttatac
     9781 atgtacttta aacttggcaa tcgacctttt tatcgaaaca gatgaaatga aaccatccca
     9841 agtcaatcga acaaacgatg atgatataca agctaatgga aaaggtgtga atgtttcatt
     9901 gatcatgaag caattaggta tcgatagtac agctttagga tttaaagcag gttttaccgg
     9961 gaattatatt gaggattatt tgcatcaaaa agatattagc actgatttta ttagcgtacc
    10021 gggaatgact agaatcaacg tttttacaca agttaataaa accaatgaag aattcaaatt
    10081 ggttaataaa ggacctgaga ttcctcagga acaaatcaaa acttttctaa atcaaataca
    10141 aaagctgcaa gctgacgatt atttgtgtgt ttctggcagt ttgccgcgag gggtctcacc
    10201 cgaaattctt attgatatca gtcgtatttg ttttgaaaat aaggtccatc tgattattga
    10261 ttcaagctat ccggaaataa ttgattgtct gccgtatcag ccgttcttat taaaaccaaa
    10321 cgaggaggaa ttgcaatctt ggtataatgt cgagcttagt gatcgtgaag gatatattca
    10381 ttatgctgaa cgcttgattg ccgatggagc gaaaaatgtt ctcctttctc taggaggaga
    10441 gggaggaata tttttaaata aggatatcgt ttattctggg aattctccta aaggtaaagt
    10501 agtcaataca gcgtgtgctg gtgacacttt gttgggaaca tttcttgcag gatatttaaa
    10561 aggtgaacag gtagatatta atttgaagaa aagtttagca gctggaagtt caaccgcttt
    10621 tcgtaaagga ttaacagatt tttcggatgt tgcagagttg gaaaaacaaa tcgaaatcaa
    10681 agagggagcg agtaaaaaat gacaacttac aaaattgttg ccgcaacagg atgtccaaca
    10741 ggaattgccc acacttatat ggctcaagaa gcattggagc aggcagctaa gcgcaaaggg
    10801 gtatctatca aagttgaaac ccatggtcaa gtagggatag agaatgaatt aacaccagaa
    10861 gaaataaaaa acgctgacgc tgtaatcatt gcagctgaca aagatgttca agcagaacgt
    10921 ttcgcaggta agcgtgtaat cgatgtgcct gtcagcaagg ggattaaaga tgctgaccaa
    10981 ttgatcgaag atgcgttggc tggtaaaggt tcaatcactg cagaaaacaa aacagttgac
    11041 gctgctggtg agaaagcgga gatgggggtt gcagcaggca ttggtcacag tatttataaa
    11101 aatctcatga acggtgtttc tcatatgctg ccgtttgtag tggcaggtgg ggtaattatt
    11161 gcaatttcgt ttgccatctg gggtgtgtac tcttttgatc ccaatgatcc tacttacaat
    11221 ccgactgccg ctatgttaaa gggtgttggt gatgcggcaa tgggaatgat ggttcctatt
    11281 ttagcagctt atatcgctga aggaatcgct aaacgtcctg gtttggttgt tgggtttgtt
    11341 ggcggactgt tagccaattc tggcggagcc ggtttcttag gcggaatttt tgcaggtttc
    11401 ttagcaggct actttatctt actattgcaa aaggtcatga aaggcttgcc taaacaattg
    11461 gatggtctga aggcgatctt cttatatccg gtgatcggtg tcttgtttgt tggtgtagtc
    11521 atgtctttat tagcaaatcc aatggaagca gtcaaccaag gaatgatgaa tttcttggca
    11581 ggattccaaa attctaatcc attaatttta gggattatcg ttggctgtat gtcagcattt
    11641 gatatgggag gtccagtcaa taaagcggct tatgttacag ggactgcgtt gttggcacaa
    11701 ggtaaccata catttatggc aggggtttcg gcagcttgta tcgcgccccc attgattact
    11761 ggatttgccg cactgttttt tggaaaatat ttcgacacga atgatcgcaa cgctggttta
    11821 gtcaacttta tcttgggctc aacgcacatt accgaaggag caattccatt tgcggctaaa
    11881 gatccgctgc gtgttctgcc aactatgatg ctaggttcat caattgctgc tgtactgaca
    11941 tatatgtttg gtgtccaagt tcccgcacct catggaggtt ttctagtatt acctgttgtt
    12001 actggtaagg ttgcatgggt agggtcgatt ttacttggtt caatcgttgg cggagtaatt
    12061 ttagggttga tccaaaaacg tagtgtagaa aaacgtgaag cagaagcagc tgctgttgaa
    12121 atgaaagaag caaccaacaa ctaaaggtta ttctagaaag aaggatttca aatgactttg
    12181 atcaaaacaa ataaagtttt tatgaataaa aattttacga cgcaagatga ggtaatgaac
    12241 ttccttgcag atcagtcaat tgtcttgagc attagtgaag accgagatgc agtttacgaa
    12301 aaattgatgg aaagagaaga agaaagcacg actggtatga tggatggttt tgccatccct
    12361 catgccaaag atgctagtat caaggatgcc gatattgtga ttgttcggtt aaacggtaag
    12421 gtcgattgga aaagtttaga tggagaagga acggattttg taatcgctct gtttattcca
    12481 gattcagagg ctggaacaac ccatctaaaa tttttatctc aggtagccag acttttgatg
    12541 cacaaagatg tgacagaagg tctgaaacaa gcccagtctg ctgaagaaat agctgaactt
    12601 ttaaaccaaa aattagaaga aaactaaaaa ggaagtagtg aaatcatgcc tttaacagaa
    12661 aaaaagaaaa cttatattcg tcaactaagt aatgaaagtg gcatcatcag tgcgttagca
    12721 atcgatcaac gtggtgcctt gaaaaaaatg atcggtaaat ttcaaaataa atcagcaacc
    12781 gatgaacaaa tcatcaattt caaaaaaatc atttcttctg aattgactcc atatgcttca
    12841 gcaatcttat tagatccaga atatgggttg ccggcagccg aagttcgcga taaaaatgct
    12901 ggtctgttgt tggcctatga aaaaactggt tacgatgctt caactccagg acgtttaccg
    12961 gatattttat ctatttggtc tgtaaaacgt ctaaaggagc aaggggccga tgcttgtaag
    13021 ttcttgttat actacgatgt agatgaatct gatgacatca atgaccaaaa gaaggcgttt
    13081 atggagcgga ttggttctga atgtgttgct gaagatttgc catttttctt agaattggtt
    13141 tcttatgatg ccaacgatcc tgatacttct tcgaaggaat atgccttgaa aaaaccgcat
    13201 aaagtaaatg aaatgatgaa ggaattttct aagcctcgct atggagttga cgttttgaaa
    13261 gtggaagttc ctgttaatat gaactatgtg gatggttaca ctaaaaatga agttgcttat
    13321 accaaagaag aagctgctgc ttatttcaaa gaacaatccg agactaccaa cctgccattt
    13381 attttcttga gcgcaggagt aactgctgaa ttgttccaag aaaccttgaa atttgcaaaa
    13441 gaggctggct ctacattcaa tggggtactc tgtgggcgcg caacttgggc gaatggtgta
    13501 gaaccattcg ttactaaagg tgaatatgct gccaaagaat ggatgcagac acaaggacgc
    13561 aagaacatcg aagagctgaa taatgttttg aaagaaacag caacagctat taaattgtaa
    13621 gaaagcttta actataacct taagtttgta acctaatgat ttttaataat caatttgagc
    13681 ttttaaataa agaattggaa gcgtcccgac gcttcctttt tcgtttgttt ttaaggcatg
    13741 ataggttcta tgttgtcgtt caataagcgc aagaaggtca gcattttttt cttagagtat
    13801 gcaaaattgc ttttctaccc aatgatttaa aacggagagg cacccttgga tatggtagat
    13861 ggtgaagtaa cgcatttctt ctgtgatcac ggtttttttg tcttcttgta gccagatttt
    13921 taaataattt tcttcgatca gattatttaa tctcttttga aattggatat ctccgtggtt
    13981 gatgacgaat aacttgtata cgaacgtatt cgtatagaca tagtcgatca attgagcgta
    14041 tagatcctcg tagctatgtg tcgtatcgta ggaaatgatt tcatccaagt cttggaaaaa
    14101 gctattttcc agtttttcat aaagatcata gatatccgta taatgtgtat aaaatgtcga
    14161 acggtgaaga tcagcagttt cggtcagttc acggatggtg atgttttgaa tttttttatc
    14221 tactaataat tttgataatg cttctgctaa cgctttttct gtttttttga cgcggcgatc
    14281 ctcttttttc attttgcagc ctcctaatca acataatcaa ggaattgtgt cggttatccg
    14341 acaatttcac cgctatctga acatagataa ccgacacctt gtctattata atataagtat
    14401 agcatatcaa tatttaaaga ataggagaat aagtgatgga attagccttt aagcaactgg
    14461 aaaagaaaga ctacaaaaaa gccattcaat tcgcgattaa aggtatgcac tttgattggt
    14521 ataccaagaa caaactcctt ttgaatcttt atggaagata cttttggtat ctagaattga
    14581 atcgcgcgac acaaatcatt gctttatatg cggatgatga gttggcaggt gttttattag
    14641 cagacatcaa aggaaaatca aaaaagcatc attcatttag tcaaaagtta tatgttaaac
    14701 tatttgattt cttacaaaat gcatttgtga aagacagcaa aggcgggtat gacgacacca
    14761 atgacgaatt gctttcagac tatttaaaaa aacattcccc agatggagaa attgtctttt
    14821 tagctgccaa ccctgattta aagatcaagg ggatcggtag taaattatta aaagaatttg
    14881 aacgccgaga gcaaggaaaa gaagtatttc tgttcacgga tagcggctgt acgtatcaat
    14941 tttatgaacg acgcggtttc gaacggaaaa aagaaaaaga taacgtcatg aagatcctta
    15001 ataaagaagt agatctccgc tgtatgttat atagtaagat cattcagtaa tgttttttac
    15061 tatcagaaag cagaagtacc acgaaggtca atggaaaaag cgagtaattg atccatttag
    15121 atgaagatag gtgtagtctc aaagatatta gctggcaaag atatccccag ttaaaaatag
    15181 atttctgtat ccaacaataa aaatattttt gtatttatta gtatatatac tttacaaaaa
    15241 tttgtatata tactaatttt tttgtaggat caatgaaatg aggggaaaat atgccaaaat
    15301 ataaagaaat agccttggct ctaaaagaga ggatcgttga gggaaaatat caggaaggtt
    15361 ctaaattacc tgagcaagaa agtctggcta gagagtttaa aacaagccgt gttactatcc
    15421 gcaaagctat acaactcctt attgatgaag gtttgttata tacgagacgt ggatctggta
    15481 cttttatacg aagcaatatc agacaggaca atgagaatgt cactcaagta aataatgtat
    15541 ttggcacttc ctcacaagaa aatacaaatg tgacaagtaa aataatccgt tttgatgtcc
    15601 ggtttccgac agattatgaa aggcggcttt ctgcaaccta cttccgatcc ccttcctgca
    15661 aatcatgggt ataaaaaata ggcatattaa gcgggctagg aggagaaata ttcacatatc
    15721 attttttcat tccgcaagct gcgtcaagtt atttggaatt tgtagaacag atgagagagg
    15781 tagaagcagc actacaaact acttttcaat aagtaggaat tatagaaatt ttactcaaaa
    15841 aatcttcatt aaaaaatctg actaaataag ttgagaaaac agaatatact tcaatatcca
    15901 gtgatttata aatatcattc actcatgata cccagctccg tatcataagt gatgcggttt
    15961 ttatcattga tcctaaaaat cttagcaaga caaaaagtac gtaagaaagt aaagactaaa
    16021 tacttcggag gaacaaatga ataacaatga taggttattg cgattgcgtt atgcgattga
    16081 tttgaaagat acggatttga ttaaagcttt tgagctaggc ggtgtaccac tgactaaaga
    16141 agaagctcag gcggtactga ccaaagtaca ggataagaac aaagataatg cagaaaataa
    16201 tgtgtatgaa aaaaccatca acaaccaagt ttttgacgca ttcctaaatg gcctgattct
    16261 cttggcgcgt ggtccgcaaa aagataagcc gatagtagac acgccgcaaa aaagcaagga
    16321 aatcaaatat atcaacaatg tgttgctaaa aaaattaaaa atcgccttgt ctatgaccac
    16381 tgatgatatt ttagaagttt ttgctgaggc agaactctat ccatcaaaag gcgaaatcgg
    16441 ggcatttttg agaaaagaag gccaacgaaa ttttaagcct tgcggagata aatatatgcg
    16501 taattttctc aaggggttag gaatttataa tcggagaaaa gtataaagaa atttcatgag
    16561 aaatcaaaac aatggttgtt ttgatgagag ctagtttttg tatgagatta aaatattatc
    16621 ttaatcgagg agctggattg gttttatgaa tgatgaatat aagaatgatg aagataagat
    16681 gctttttgaa gagatagaga atcgatgtag gctcaatttt gagcttcggg gaaagatgtc
    16741 tttaatacaa caaaaaaagt atttagcgaa taaatcagag tttactctgg gacatgttga
    16801 aaaattaatc tcggattgga ttagtagtag aagcgaattt acaaaaataa aacagccgat
    16861 taaatttgac atgaaaaaac ttttgttaaa taagagtgag ataggtaata gagatcaata
    16921 tattagagct aaagggcaag aaataattga ttcattaggt gaagtgaggt catacaatta
    16981 tttgtatgta acacatagag ctgatggaat ggtcattact gtggggaaat cttcttctaa
    17041 tgatattttt ttagatggag atctttttta tcaactcaat acaaatcact tgtctggcac
    17101 tgaaaatata attttaagga cggaatatgg caatgaaata tttgcaaaat atgatgagat
    17161 tttaaaaaat tatttggact gggcttggat aattccggtt gagtcaggag atgctaagaa
    17221 actggagaga ttattgggcg atgaactaat taataaaaaa gtaccaattt taaattatta
    17281 ttcccataga caataaaagg ctatttttca tgataatact agttataact gtaaaaatgg
    17341 aagaaaatct gggaaagaag tttttaaata agtgtattgt tcgattttaa taaataataa
    17401 gcaataccat ccgtctgtgt tgcgcaacat cgtactacaa tgaatatttt ttcacaaaaa
    17461 gaaaatcatc catcgaatga atggaaggtt ttagtataca cgaggttaat gtcccctctt
    17521 ttataatcag aggagtgagg ttttttctat gaatgtttct attttcttat tgctagtcta
    17581 gtttctctta cgatgaaatt gggattctat tccagttttt attgatttgc tgataaaata
    17641 acttgaatgg tggatataaa catgataaat aagagaagaa taacaaaaca aggttgtgaa
    17701 atgattctca aataagtata atagaaagaa aaaggaggaa gaatcaatga aaatagcaat
    17761 tgcaggcgct ggcgcaatgg gaagtcgctt tgggttaatg cttcaccaaa gtgggaacga
    17821 agtattatta atcgatggct gggcagaaca tgtccaacaa atcaaagaac atggattaca
    17881 agcaaatttt aatggtaaag aggtagaagc aaaactacca atcgttcttc aatccgaagt
    17941 agaaaaagaa gatcaagttg atctgattat tctatttacc aaagcgatgc agctggaaaa
    18001 aatgctgcag gatatccaat cattaatcaa aaaagataca gaggtcttat gtctattaaa
    18061 tggtatcggg catgaagata ttattgagaa atttgtacca atggaaaata tctatattgg
    18121 aaataccatg tggacggcag gtcttgaagg tcctggtcag gtcaaattat ttggaagtgg
    18181 ttcagtagaa ttacaaaatt taggtgatgg aaaagaagca gctgcaaaaa aattagcaga
    18241 caaattgtct gaatcaggat taaatgctca tttttctgac aatattcact attctattta
    18301 tcgcaaagca tgtgtgaacg gaacaatgaa cggactatgc acaatcttag acgttaatat
    18361 ggctgagcta ggaaaaacat cgactgctca taaaatggtg gcgacgattg tcaatgagtt
    18421 tgccaaagta gcagcagtag agaagattga actagatgtc ccagaagtca ttgcacattg
    18481 tgaatcttgt tttgacccgg aaacaattgg gttacattat ccttcaatgt atcaagactt
    18541 gattaagaac catcgattaa cagaaatcga ttatatcaat ggcgcaattt ctagaaaagg
    18601 gaagaaatat ggtgttgcga cgccttattg tgatttcttg actgagcttg tccatgcaaa
    18661 agaagatagt ttgaacgtaa aataaaggag agattgaatg gaaacttcac aaaaaatatc
    18721 accgaaaatt tttctgaata aagtcctcgc tggtacagca acaggaatca ttattggatt
    18781 gattcctaat gcagtattag gtgctatttt aaaatatttt agtgcgtatc cctttgcggt
    18841 aacgatcgca cagttagcag ttatttttca attagcaaca cctttgatca ttggtggttt
    18901 gattgcctta caatttggat ttgatccgat gagaatgatg gttgtagccg gagcttcttt
    18961 tgttggttct ggtgtagtca aattcacacc tgatttgggt gaaacgggtg tttacgttgg
    19021 tgctggtaca ggcgatatta ttaatacgat gctgacagca tcgattgctg tcgggctgat
    19081 tttactgatt ggaaatagtt ttgggtcagt gtcgattgtg ttaacaccga ttgttgtagg
    19141 tgtaggtgca ggtttacttg gtttttattt attccctttt gtaacagcaa ttacttctgc
    19201 aattggatca ttgattaata acttcactac cttacaaccg ctgttaatgt ctatcttgat
    19261 tggttgttcc tttgcctttt tgattatctc tcctatttca acagtagcaa tcggaatggc
    19321 aatccaacta gatggtgttt ctgctggtgc ggctgcgatg ggcgtagctg caaccacttt
    19381 tgttttagta gtgaactctt ggaaggttaa taagtcaggt gtgacaattg cgattgcttt
    19441 aggcgctatg aagatgatga tgcctaatct atttagaaaa cctgtgattc ttattccttg
    19501 tttagttagt gcagtgatta cagctattcc tgtcgctcta ttttctattt caggtacgcc
    19561 tgcttctgct ggtttcggtg tggtcggact tgttggtccg ttggcttctt tagatgcagg
    19621 gctaaatggc tttcttgttg ttctttgttg gttagtcatc cctgtttttg cggcactaat
    19681 tactcaattt ttatgcgaga aagtatttaa actgtatgat cgagaagaag tcttcaaatt
    19741 tttaggctag tagattttac taagaaattt tcaatcaatg gatgcgtaag tgagatgtca
    19801 acaacatctg acttacgttt ttttgccaaa atatggtttt tattttttat tattaggtgt
    19861 gattagtgat ttatcgacac cgtatatcct tggccttttt tatcccaaac tgaatcaaat
    19921 gactaccgta attagtgtgt ttggtgacgt agatagtcca gtcagacgtg ctttcttggt
    19981 ctggtcagtt gtatctggac ttttttttgt tctttcgttg cctgcgttgt atcacctatt
    20041 tgttggaaca tcaaaaacac ttgcaatact tgctgttgca acagttggtt tatatgggat
    20101 aggtgattgt atttttactg ggttattcag tatcaatacg aatgaaagct cttggaatct
    20161 atcgacttgg atccataata caggttctgg gctaggctat gcaggctttc tacttttccc
    20221 attgttactt gttctgcttt atcgtcaaa
//
LOCUS       DACDNO010000054        17714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDNO010000054 DACDNO010000000
VERSION     DACDNO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.054"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            3..815
                     /locus_tag="IWI22_002043"
     CDS             3..815
                     /locus_tag="IWI22_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9169424.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            812..1612
                     /locus_tag="IWI22_002044"
     CDS             812..1612
                     /locus_tag="IWI22_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9169425.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1773..3041
                     /gene="rseP"
                     /locus_tag="IWI22_002045"
     CDS             1773..3041
                     /gene="rseP"
                     /locus_tag="IWI22_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9169426.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3109..4818
                     /locus_tag="IWI22_002046"
     CDS             3109..4818
                     /locus_tag="IWI22_002046"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9169427.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5030..9382
                     /locus_tag="IWI22_002047"
     CDS             5030..9382
                     /locus_tag="IWI22_002047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9169428.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9526..9999
                     /gene="rimP"
                     /locus_tag="IWI22_002048"
     CDS             9526..9999
                     /gene="rimP"
                     /locus_tag="IWI22_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9169429.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10023..11198
                     /gene="nusA"
                     /locus_tag="IWI22_002049"
     CDS             10023..11198
                     /gene="nusA"
                     /locus_tag="IWI22_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9169430.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11220..11513
                     /locus_tag="IWI22_002050"
     CDS             11220..11513
                     /locus_tag="IWI22_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9169431.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11510..11821
                     /locus_tag="IWI22_002051"
     CDS             11510..11821
                     /locus_tag="IWI22_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9169432.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11834..14140
                     /gene="infB"
                     /locus_tag="IWI22_002052"
     CDS             11834..14140
                     /gene="infB"
                     /locus_tag="IWI22_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9169433.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14167..14514
                     /gene="rbfA"
                     /locus_tag="IWI22_002053"
     CDS             14167..14514
                     /gene="rbfA"
                     /locus_tag="IWI22_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9169434.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14622..14843)
                     /locus_tag="IWI22_002054"
     CDS             complement(14622..14843)
                     /locus_tag="IWI22_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9169435.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15291..17654
                     /locus_tag="IWI22_002055"
     CDS             15291..17654
                     /locus_tag="IWI22_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP9169436.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWELFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6035 a         3146 c         3903 g         4630 t
ORIGIN      
        1 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
       61 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      121 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      181 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      241 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      301 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      361 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      421 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagt agagcagaga
      481 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      541 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      601 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      661 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      721 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      781 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtgatca cagcagctgt
      841 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
      901 agcaatctta gcggtagtag gagtttacga gctgttccgg atgaaaggat tgacactctt
      961 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1021 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1081 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1141 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1201 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1261 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1321 aacgattgaa ggtgcgctcg gtggtatcgg tagtgctgtt gtagtcgcat ttctttattt
     1381 attgctatac ccagcaaaag aactattcaa tcatggtatg tttgtcatgc ttctgatgac
     1441 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1501 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1561 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1621 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1681 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1741 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1801 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1861 gaatcctcgt ccgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     1921 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     1981 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2041 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2101 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2161 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2221 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2281 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcgtgttat
     2341 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2401 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2461 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2521 caggtaaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2581 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2641 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2701 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2761 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2821 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     2881 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     2941 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3001 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3061 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3121 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3181 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3241 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3301 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3361 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3421 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3481 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3541 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcata ttctttccat
     3601 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta taccgaagta
     3661 ttcaagcgtt gtggcttaga atttcgctcg atcatcggag acggcggcgc aatgggcggg
     3721 aaagactcaa aggaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3781 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3841 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     3901 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     3961 gcggatgaac aacctgtcct tgttttggta cgtggagacc atgaagtcaa tgacgtaaaa
     4021 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4081 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4141 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4201 ttccactata aaaatgtgaa tccagaacgc gatttcactg tgctttctta tgaagatctt
     4261 cgttttgtac aagaaggaga tccttcacca gacaacaacg gtgttcttgc ctttactcga
     4321 ggaatcgaaa tcggacatat attcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4381 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4441 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaattgg
     4501 ccaaaaggaa ttgcgccatt tgatctgcat atcgtccaaa tgaatctaaa agacgaatac
     4561 caaacaagcc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4621 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4681 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4741 aaaactggcg aaatggttga agtacgtaaa gaagaattgg cgaatacttt accaatcctc
     4801 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4861 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     4921 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     4981 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5041 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5101 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5161 ggaatttacg ctacattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5221 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5281 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5341 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5401 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5461 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5521 agtggatgag cagcaagcgg aacgtgtatt gaaacttttt gaagaacgca aacaagaaca
     5581 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5641 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cagctaggac gaaatattcc
     5701 agccgatgaa ccaacgacac caatgatcag tatcgttgaa gaagaacgaa gagtcacact
     5761 tgaggggtac gtattcgaca aagaagtcag agaactacgt tcaaaacgca agatcttgac
     5821 tttgaaaatc acagattata cttcatcatt tatcgtcaaa aaattctcga acggcgaaaa
     5881 agatgaacaa gtatttgatg cgatcagtgt cggtagctgg ttaaaggtac gaggaagtat
     5941 tcaagaagat acgtttgtcc gtgatttggt catgaatgcg caagatatca tagaagtcaa
     6001 acatacacct cgcaaagatt atgctcctga aggggaaaaa cgagtagagc ttcatgttca
     6061 tagcaatatg agtacgatgg atgcaacaaa cagcatctct gatcttgtgg cacaagcagg
     6121 aaaatgggga caccgagcta tcgccattac agaccatggt ggtgcgcaag cctttccgga
     6181 agcacatagt gctggaaaaa aagcaggtgt gaaaatctta tacggcgtgg aagcgaatgt
     6241 cgtagatgat ggtgtcccaa ttgcttataa tgatgcacat gaagcattga gtgaagccac
     6301 atacgtcgta tttgacgtgg aaacgacagg attgtctgca gtatatgaca cgatcatcga
     6361 gctagccgca gtcaaaatgt ataaaggaaa tgtgatcgaa agctttgatg aatttatcga
     6421 tccaggacat ccattgtcac gaacaacggt agatctgact ggtatcacgg atgaaatggt
     6481 tcgagggtca aagtcggaag aagaagtatt gcggatgttc cttgaatttt caaaagatac
     6541 aattttggta gcccacaacg cggcttttga tatggggttc ttgaatacta gctacgcaag
     6601 gtatggtatt cctgaagcgg ccaatccagt cattgatacg ttggaattag ctagatatct
     6661 gtatcctcaa ttcaaacgtt tcggattagg tgtgttatct aaaaagtttg gtgttagctt
     6721 ggaacagcat caccgagcaa tctatgatgc tgaagcaact ggtcacttag catggatctt
     6781 tgtgaaagag gcaatggaca atcataacat gctttatcat gatcagctaa atgagcatat
     6841 cggagaagga gattcttaca aaagggctcg acctttccat gtaacgattt tagcaaaaaa
     6901 tcaggcagga ctgaaagatt tgttcaaact gatttcaatg tcgaatgtcg aatacttcga
     6961 acgcgtccca cgaataccac gctcccaatt gaaaaagatg cgtgaaaatc tattgatcgg
     7021 ctcagcttgt gataagggag aaatattcga agccatgatg caaaaagggg tagaagaggc
     7081 aagaaaccga gcaaaattct atgattatat tgaagttatg ccaaaagcgg tctatgcgcc
     7141 tttgatcgag caagaattgg taaaaaatga acatgatttg gaagaaatta tccaaaattt
     7201 ggtagaaatc ggaaaaagtt tggataaaat cgttgttgca acaggtaatg tacattatct
     7261 gaacgaagaa gatgccattt accgaaaaat tttgatcaat tctatgggcg gagcgaatcc
     7321 gttgaatcgt catagtttgc cggatgtcca tttccgaaca accgatgaga tgctaactgc
     7381 atttcacttt ttaggggaag aaacagcgaa agagatcgta gtagaaaata cgaataaaat
     7441 cgcagatatc tgcgaagaag tgattccagt caaagatgaa ctttatacac cgaagattcc
     7501 aggttcagaa gatgaaatat cagaattgag ttacacaaaa gcaaaacaaa tgtacggaga
     7561 tccgttacct gagatcattc aaaaacgatt agaaaaagag ttgaattcta ttaatggtaa
     7621 tggcttctcg gttatttatc tgatcgccca aaaactagtg cataaaagta atgaagatgg
     7681 ctatctagta ggttcacgtg gctctgttgg ctctagcttt gttgcgacga tgacaggaat
     7741 cactgaagtt aatccattag cccctcatta ttattgtcca gagtgccaat attccgagtt
     7801 ttttgaagat ggtacgtatg gttctggatt tgacatgcca gaaaaacaat gtccaaaatg
     7861 tggtgcacgc ttaaacaaag acggtcatga tattccattt gagacatttt taggattcca
     7921 tggagacaaa gtgcccgata ttgatttgaa cttctcaggt gattatcaag cagaagccca
     7981 taactatacg aaagtattgt ttggcgaaga ctatgtttac cgtgccggaa cgattggtac
     8041 agtcgccgac aaaacagcat atggctatgt aaaaggatac gaaagggaca ataatctgca
     8101 gtttcgaagt gcagaagtcg acagattagc aaaaggcgct actggagtca aacggacaac
     8161 cggccagcat ccagggggga tcatcgttat tcccgattat atggatgtct atgattttac
     8221 gccgatccaa tatccagcag acgatcaaaa ttcagaatgg aaaacgactc attttgattt
     8281 ccactcgatt cacgataatg tattgaaatt agatattcta gggcatgatg acccaactgt
     8341 tattcggatg cttcaagatt tatcagggat cgacccacaa acgatcccta cggatgaccc
     8401 ggaagtgatg cggatatttg ccggtcctga agtacttgga gtaagtcagg aacaaattta
     8461 ttccaaaaca gggacattag gtattccaga attcggcact cgttttgttc gagggatgtt
     8521 agaagaaacg catcctacaa cttttgcaga actactgcaa atttccggac tatctcatgg
     8581 tacagacgta tggttaggaa atgcggaaga attgattcga cgaggagatg cgacactggc
     8641 ggaagtaatc ggctgtcgtg atgatattat ggtttatttg atccatgctg gattagatag
     8701 cgggatggca tttaaaatca tggaaactgt tcgtaaagga caatggaaca agatccctga
     8761 tgaattgcga gaaacctatc tatcggcgat gaaagaaaat aatgtgcccg actggtatat
     8821 cgattcttgt tcaaaaatca aatatatgtt tccgaaagcc catgctgcag cttacgtatt
     8881 gatggctctt cgtgtcgctt atttcaaggt ttatttccct attttgtatt actgtgcata
     8941 cttttctgta cgtgcagacg actttgatct agtctccatg tgcaaaggaa aagatgctgt
     9001 gaaacaagca atgaaagaaa tcacggacaa gggattagat gcttccgtaa aagaaaagaa
     9061 tcaactgaca gtgctggagt tagccaatga aatgctggaa cgaggattca aatttggaat
     9121 gattgatttg tataaatcgg atgccgttaa ctttgttatt gaaggtgata cgttgatcgc
     9181 accatttaga gctgtcccaa gtttgggaac caacgtggcg aaacaaatcg ttgaagcaag
     9241 aaaagacggt ccatttttat ccaaagaaga tcttgccacc cgcggtaaag tttcaaaaac
     9301 attgatcgaa tatatgaacg acaatggcgt attgaaagat ctaccagatg aaaatcaatt
     9361 atcgttattc gatatgctat aagcatctgt tttcctgtct gcttgtaatt ttaagcagag
     9421 tatggtatag ttcttatagg taatgatact aactgtgagt gagcggattt ttcgctcact
     9481 ctttttaatg gaattacgca cttaattttt aaggaggcga aacttttgag tagcgtcgtt
     9541 gaaacagtca cagaaatggt gacaccgatt ttagatgaac aaaattttga acttgtcgaa
     9601 gtcgaatttg taaaagaagg aaaaagctgg ttcttacgag tatttattga caaagaaggc
     9661 ggtatcgata tcgaagaatg cgcctttgtc agtgagaaac tgagtgaaaa acttgatgca
     9721 gcagatccag atccgattcc tcaagcttat ttcctggagg tatcctcccc gggagcagag
     9781 cggccattga aaaaagaaag cgactatgaa aaggcacttg gtgattatgt ccatgtctcc
     9841 ttataccaac cagtagatgg cgaaaaacaa tatgaaggat ttcttcaatc atttgatgct
     9901 gaacagctta cattgaaaat acgcataaaa acacgagaaa aagaattgac ttttgaccga
     9961 aaaaatattg caaaagctcg cttagctatc caattttaat cacggagagg aatcagaaaa
    10021 aaatgagtaa agaaatgtta aatgcgttag acgctttaga agctgaaaaa gggatctcaa
    10081 aagaaatcgt gatcgatgct ttagaagcag cattggtttc tgcatacaaa cgtcactatg
    10141 gccaagcaca aaacgtcgaa gtggaatttg atcagaaaaa aggaaagatc catgtctatg
    10201 cggtaaaaga agtaacggaa gaagtgatgg attcacagtt ggaagtctca ttgaaagatg
    10261 cacttttaat caatccagca tatgagatcg gtgatacgat ccgttttgaa gtaacaccaa
    10321 aagattttgg acggatcgct gcgcaaacgg ctaaacaagt tattttacag cgtgtacgtg
    10381 aagcagaaag aacgattatt tataacgaat tcagtgctta tgaaaaagac atcatgcaag
    10441 gaatcgtcga acgccaggat aaacgttata tttatgtcaa tcttggtaaa attgaggcag
    10501 ttctttctaa acaagatcaa atgccaaatg aattttatca accacatgat cgaatcaaag
    10561 tttatgtttc ccgagtagaa aatacgtcta aaggtccgca agtatttgtt agccgcagcc
    10621 atccagacct attgcgtcgc ttatttgaac aagaagtacc agaagtctat gatggaatcg
    10681 ttgaaattgt cagcatcgcc cgtgaagctg gcgatcgttc aaaagtggca gttcgctcaa
    10741 atgatccaaa tatcgatgca gtcggtacat gtgtcgggcc aaagggacaa cgagttcaag
    10801 caatcgtcaa tgaattaaaa ggcgaaaaca tggatatcgt cgaatggaat gaagatccag
    10861 ctgtattcat cgcaaatgca ttgaatcctg cacaagtcat ggatgtgatc ttcgatgaga
    10921 caaatccaaa ggcctgcaca gttgtcgttc cagactacca attatcgcta gcaatcggta
    10981 aacgcggaca aaatgcacgt ttagctgcta aattgactaa ccataaaatc gatatcaaat
    11041 cagaatctga catgacggaa ttttatgaaa atcaagaaca acaaaaagaa acagaagaac
    11101 tgcatgatga agcaatcgtt cagtcagatt taacagacga tgaatatgaa acaattgcat
    11161 tcaacaatga aacggtcgaa gaaaaaccag aagcttaact ttagtaagga ggcaaagaga
    11221 tgaaacaaag gaaaattcct ttacgcaaat ccgtggtttc aggtgaaatg aaacctaaaa
    11281 aagagatggt ccgaatcacc cgttcaaaag aaggggaagt atcgatcgat ccaactggaa
    11341 aaatgcctgg acgaggagcg tatgtctcac ttgagccgga agaagtacaa caagcttggg
    11401 ataaaaaaat tctcgatcga gtactcgaaa caacattaac agatgagttt tatcaagaac
    11461 ttcttgacta tgtcactcac caaaaagcac gtaaagagtt atttggaaaa tgaatacagt
    11521 taatcggcaa aaagccatga atctcatcgg tctagcgatg cgcgcgggaa aactaatcac
    11581 gggtgaagaa ctgaccattg ctgaaatccg caaacaaaaa gcaaaattgg tgtttgtggc
    11641 aaccgatgca agtgagaata caagaaaaaa gataaaagat aagagttcat attatgaagt
    11701 tccttgcttt gagctatttt ctgaagcaga aatcacgcag atgatcggaa aacctcgcaa
    11761 ggtattcgga ataatggatg ccggatttgc gaagaagacc aaggagctaa ttgaaggtta
    11821 ggaaggtgat tgcatgggca acaaaagaat ctatgaatta gcaaaagaac tcaaaaaatc
    11881 tagtaaagac gtcgtggaaa aagcacaaca acttggcatc gatgtgaaaa accatatggg
    11941 tgcgattact agcagtgatg agaagaaatt gcaacaagcg ttcaaaaacc caagcacgaa
    12001 acaacaagct gcacaacaag caagcagaaa accagccaat caacagccga acgatcaacg
    12061 taaaaaagaa acaacgaaca atcaacaaaa aaataaaaat aaccgcagtt atcaagatcg
    12121 cggtcaaggg agcggtcaag tgaatcaagg aaaaaaacca acaacaaacc aaaataatac
    12181 gggaaatacc agcagccaaa atcgtcaagg aacgagccaa ggcggcaatc aaaaccgtac
    12241 taacaataac aacaaaccaa acaataacaa tcgcggcaaa tttaataata ataaccgtaa
    12301 tcgttataat aaaaaaggta aaaaaggcaa acagcaacaa tcaactaaac cagctgtacc
    12361 accacgcaaa ttccgtgaat tgccagatgt attagagtat actgaaggta tgaacgtagc
    12421 agatattgcg aaaaaaattc atcgtgagcc agcagaaatc attaaaaaat tatttatgct
    12481 tggtgtcatg gtcaatcaaa accaagcact ggataaagat acgattgaac tgttagcgac
    12541 ggactatggc atggaaccac aagaaaaagt ccaagttgac attgctgata tcgataaatt
    12601 cttcgaggca gaagaagtcg atccagataa attagtctca cgtccgccag tagtaacgat
    12661 tatgggacac gtagaccacg ggaaaacaac cttattggat acattgcgcc attcacgtgt
    12721 aacaagcggc gaagccggcg gaatcacaca gcatatcggt gcctaccaga tcgatattga
    12781 tggtaaacca atcactttct tggatacacc aggacatgcg gcctttacaa gtatgcgtgc
    12841 acgtggagca agtatcacag atatcacgat cttagttgtt gctgcagatg atggcgtcat
    12901 gccacaaaca gttgaagcaa tcaaccatgc caaagctgca ggcgttccaa tcatcgtcgc
    12961 tgtaaataaa attgataaac caggtgcaaa tcctcagcat gttatgcaag aattaagtga
    13021 gtatgaatta attcctgaag catggggtgg agaaacgatt tttgttgaga tctcagcgaa
    13081 attcggccaa aacatcgaag aattgctgga aatgatcctg ttagtcgcag aagtagaaga
    13141 tttaaaagca gatccaacac aacgcgcaat cggaacagtg atcgaagcac gtcttgataa
    13201 aggtaaaggt cctgtgtcaa ccttacttgt ccaagaagga acacttcacg ttggtgaccc
    13261 aatcgttgtc ggtaatactt acggacgtgt ccgtgtaatg acaaacgatc ttggccgtcg
    13321 cgataaagaa gcaggacctg caacgccagt agaaatcact ggcttgaatg atgtacctca
    13381 agcaggtgac cgtttcgtag tcttcgaaga cgaaaagaca gcacgtgcag cgggtgaaga
    13441 acgcgcaaaa cgtgcattat tggaaaaccg tgctgccaca agccgtgtga ctttggacaa
    13501 cttatttgaa agcctgaaag aaggcgaatt gaaagaagtt aacgtaatca tcaaagcaga
    13561 tgtacaaggt tcagcagaag cactggctgc atcattgaag aagattgatg tggaaggtgt
    13621 ccgtgtaaaa atcgtccatt cagctgtcgg agcaatcaat gaaagtgatg taaccttagc
    13681 agcagccagc aacgctatta tcatcggttt caacgttcgt cctacaccgc aggcaaaagt
    13741 gcaagctgat actgaagaag tcgatattcg tcttcaccgt atcatctata aagccatcga
    13801 agaaatcgaa actgcgatga aagggatgct agaccctgaa tttgaagaaa aaatcaccgg
    13861 tcaaatgatc gttcgtgaaa ccttcaaagt ttctaaagtt ggtacgattg caggtgccta
    13921 tgtaacagaa ggatatattc gtagagatag cggtgtccgt gtaatccgtg acggaatcgt
    13981 aatctatgaa ggccaacttg caagtctgaa acgattcaaa gatgacgtga aagaagtcaa
    14041 aatgggttat gaatgtggtg cgatgattga aaaattcaat gacatcaaag tagatgacgt
    14101 aattgaagga tttatcatgg aagaagtcaa aaatgactaa aaactgataa aaggaggcca
    14161 atcattatgg ctaactaccg tgatcgccga gtcggtcaag aaatcttaaa agaagtcaat
    14221 gatattttgc gcaaaaaggt ccgtgaccct cgtgtggaga acgtaacgat cacagatgtg
    14281 aaagttacgg gggacttaca gcaagcaacg atctattaca gcattttatc tgacttagca
    14341 tcagataaga aaaaagcaca agatggattg aataaagcat caggattgat tcgtcgagag
    14401 ttagggcaca atctaagtat ttataagaca cctgaattgt tctttgaact tgatgaatca
    14461 gttgtctatg ggaaccatat cgatgaattg cttcgcgact tgaacaaaga ctaatcaaaa
    14521 aatcccgcaa atcctgtgaa atcaggggtt gcgggatttt tttgttttca ttaagacgta
    14581 ttacctaaga gttccatgac tttagtctgg tagcgtgtca gctaaccaaa aaagagaaaa
    14641 agaaaagtaa aaaattcgac tttctttttt ctatagtata taaatttttg catatagata
    14701 aagatcaaga taccgataac ggaagctaga ttgtaatcta ttttagtgac agtcaggtat
    14761 agccaagtaa ctagaagatc agtggaacct gcaccataaa agtttgagtt tgtcggtccg
    14821 ttgacagtta aaagaaaaat cacgttgaag ttattgatat ttccgattac gccgcttcga
    14881 tttggtcacg atccaaattt tggataatcc ctgtagaaat tagcatagta gctgggatac
    14941 caattcacat attgacaaag ataacagaga cttttacata aaacggatca atcaggaaag
    15001 gaattgctcg atccagcccc agttttccaa caatgtgtta actggcctag cgtcattcag
    15061 caaattatgc atgatgagga gcgaaatgaa ctgcggaaca gccattgtga taacgacaag
    15121 ttttttggat caatgtaaaa aacgtcagcg aaaggaaaaa tttcgtggtt ttttatacct
    15181 gttcatttgg ctagatataa aatccaaaca aatgaacaga agtgcttatt aggtttcaat
    15241 tgggagtatc tgcgctagaa taagagtaat aatgaaaagg agtgttatgt atggattatt
    15301 ctagtaaaga atactttgat aaaatgacgg cttggtggcg tgctgctaat tatctttctg
    15361 ttgggcaact ttatcttaaa gataacccct tactgagacg tacactgaaa ccagaagatg
    15421 tcaaaaaaca tccaatcggt cattggggaa cgattcctgg ccaaaatttt atttatgtcc
    15481 atttgaaccg agtcatcaat aaatatgatt tgaacatgtt ttatatcgaa gggccaggac
    15541 acggcggaca agtcatggta tcgaatgctt atttagatgg aagttacaca gagatctatc
    15601 cagaagtaac ggaagatgag acaggaatgc aaaaattatt caagcgattt tcatttcctg
    15661 gaggcattgc ttctcatgca gcaccagaaa caccaggttc tatccatgaa ggcggagagc
    15721 ttggatactc tctttcacat gcagtcggag ctgtattaga caatcctgaa gtaatctctg
    15781 ctgtcgtgat tggtgatggt gaagcagaaa ccggtccttt agctggaagc tggttctcaa
    15841 atgtctttat caatccagtg atagatggag ctgttttacc gatccttcat ttaaatggtg
    15901 caaaaatagc caacccaacg atcttggcac gtaaatcaga tggagaatta gcaaattact
    15961 ttaacggact tggctgggag cttttcttta ttgaaggaaa tgatccagaa aaattaaatc
    16021 ctgttatggc tgaaaaaatg gatcaagcaa tcgagaaaat caaatccatc caaaaagaag
    16081 ctcgcctgaa gacagcaaca gatgtcgtga tgccaaaatg gccagtattg atcgtacgca
    16141 caccaaaagg atggaccggt ccaaaagaat gggacgggga accaattgaa ggaaccttcc
    16201 gagcacacca agtaccgatt cctgtagatc aagaacatat ggatcatgca gatgctttac
    16261 tccgttggct gaaatcctat gaaccagaaa aattatttga tgcacaagga cgtattttag
    16321 aagaaatcag agaaattgcg cctactggtg accaaagaat ggcaaaaaat cctatcacca
    16381 atggtggaat cgatccaaga ccgcttatta tgccagattg gaaaaaatat actttgcaat
    16441 ttgaaaaacc aggctccgtt aaagcagaag atatgaccga acttggtaaa ttcgttcgcg
    16501 agatcattga gaaaaatcca gaaaatttcc gtatatttgg accagatgaa acaaaatcaa
    16561 accgattgaa tcaagttttc aaaacgacaa atcgccaatg gatggaaaaa atcgaaccag
    16621 aaaatgatga atggctttcg ccatctggtc gcgtcatcga cagtcaatta tcggaacacc
    16681 aagatgaagg gttcttagaa gggtacgttc tgactggacg gcatggtttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatacgga
    16801 aatcacatga cctttcttgg cgaaaagatt atccttcgct gaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcgg aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaaca gaatttttta cattgtatta
    17701 tttgtcaaat taag
//
LOCUS       DACDNO010000055        17580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDNO010000055 DACDNO010000000
VERSION     DACDNO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.055"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            333..2279
                     /locus_tag="IWI22_002056"
     CDS             333..2279
                     /locus_tag="IWI22_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9169437.1"
                     /translation="MIERHVNLLKLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLED
                     YVVNYQVTIERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFG
                     DKPVSLTHLSQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAI
                     KQLVFYYANDMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLIST
                     LVIQCNRLLKGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLC
                     RQLFAHRVTNNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLR
                     LKKGIKIQNPLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIE
                     DQSKANNIIIICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISS
                     LSFDAGGIPLVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKF
                     VQSSLVKTGVQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALP
                     HANPSMVIESNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQK
                     EMVDRLLQVHDSEKLLRIFQQKGE"
     gene            2282..2737
                     /locus_tag="IWI22_002057"
     CDS             2282..2737
                     /locus_tag="IWI22_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9169438.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            2751..4079
                     /locus_tag="IWI22_002058"
     CDS             2751..4079
                     /locus_tag="IWI22_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9169439.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            4113..4397
                     /locus_tag="IWI22_002059"
     CDS             4113..4397
                     /locus_tag="IWI22_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9169440.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            4399..4914
                     /locus_tag="IWI22_002060"
     CDS             4399..4914
                     /locus_tag="IWI22_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP9169441.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            4930..5592
                     /gene="rpe"
                     /locus_tag="IWI22_002061"
     CDS             4930..5592
                     /gene="rpe"
                     /locus_tag="IWI22_002061"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9169442.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            5599..6171
                     /locus_tag="IWI22_002062"
     CDS             5599..6171
                     /locus_tag="IWI22_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9169443.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(6358..7878)
                     /locus_tag="IWI22_002063"
     CDS             complement(6358..7878)
                     /locus_tag="IWI22_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9169444.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(8121..8306)
                     /locus_tag="IWI22_002064"
     CDS             complement(8121..8306)
                     /locus_tag="IWI22_002064"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP9169445.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(8290..8472)
                     /locus_tag="IWI22_002065"
     CDS             complement(8290..8472)
                     /locus_tag="IWI22_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169446.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            8713..8931
                     /locus_tag="IWI22_002066"
                     /pseudo
     CDS             8713..8931
                     /locus_tag="IWI22_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            9426..10025
                     /locus_tag="IWI22_002067"
     CDS             9426..10025
                     /locus_tag="IWI22_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9169447.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            10089..10688
                     /locus_tag="IWI22_002068"
     CDS             10089..10688
                     /locus_tag="IWI22_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169448.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIGDPVLLQSFK"
     gene            complement(10718..11369)
                     /locus_tag="IWI22_002069"
                     /pseudo
     CDS             complement(10718..11369)
                     /locus_tag="IWI22_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(11704..12576)
                     /locus_tag="IWI22_002070"
     CDS             complement(11704..12576)
                     /locus_tag="IWI22_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9169449.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(12840..14786)
                     /locus_tag="IWI22_002071"
     CDS             complement(12840..14786)
                     /locus_tag="IWI22_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9169450.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            14971..16410
                     /locus_tag="IWI22_002072"
     CDS             14971..16410
                     /locus_tag="IWI22_002072"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9169451.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            16412..17374
                     /locus_tag="IWI22_002073"
     CDS             16412..17374
                     /locus_tag="IWI22_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9169452.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
BASE COUNT         5799 a         2917 c         3177 g         5687 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 aattattaag tcgacaagaa gactttaaac ccgcttcttt ttttagtgat aaattggcag
      421 tgtcgaccaa gaccatttat caagatattg aattgttgga agattatgta gttaattacc
      481 aagtaacaat tgagcgatta ccttctttgg gtatcaaact cataggtgat aggaaaaacg
      541 ttgaacattt gatttcatcg gtagaaaatt atcttgaaaa agatatttat tctccagaaa
      601 aaagacgtgt cgagatcatt aaagaagttt tgtttggtga taagcctgtt tctttaactc
      661 atttgtctca aagttttatg gttagtaaaa catcgttata caatgacctg agaattatca
      721 ataaaattat tggcagtgga gaagttaagt tgcagtcatc tgcagaagga attagtttta
      781 ctggtaatga acaagcaatt caacgagcaa tcaagcaact tgtattctat tatgcgaatg
      841 acatggaatc aaaaacattt tttcaagttt taaatatgtt gtttgattca aaagttatta
      901 gtgtcattca tgatttgcta ttcaatgatt acagtgaact gactgaaaag gtttctgatt
      961 actatgttcg gtcattgatt tctactttag ttatccaatg taaccgttta ctgaaaggtc
     1021 atcatttaac tgttgaagaa gaattcctct tcaatagcat tcgctatatg gaaacgtaca
     1081 ttgtggcaaa tggtttaatt gaagaattga aggcaaaatt gaatcttcca ttttatgacg
     1141 cagataaaga atatctatgt cgtcaactat ttgcacatcg tgtgacaaat aatttaaaaa
     1201 ccagcaatga agagtacgct atgagcgttc ataaattgat tgaaagaatg agtgagattg
     1261 aaaaaattga tttgactcaa aatgatcgat tatatcaatc cttattgtat catgtgccag
     1321 caatggtcct tcgtttaaaa aaaggtatta aaattcaaaa tccgttatta gaaaatatca
     1381 aatctcaata ttcagaactc tttagcatag tatggtacgc catgtccatc cttgaagaag
     1441 aatttgatgt gactttaacg gacgatgaag tttccttaat tttgattcat tttcagattg
     1501 ctattgaaga tcagtccaaa gcaaataata tcatcattat ttgccaatac ggtatttctt
     1561 ctgcgcaatt aatttacaat aaagttcgac ggtttttacc agcaaaagat aatgtagaaa
     1621 tatcaacttt agataagttt catttagctg aaattgggga agttgatttg attatttcct
     1681 ctctttcctt tgatgcaggt ggtataccct tagtaagggt atctccattg gtaagtaaaa
     1741 cagattatat gaatattatg gaagcttata ctaaatattt aatagaagaa ccttcgcgat
     1801 ttattcaaaa tgaactgatt cctaaagcgg ttaaagttcc caccatatct aaatttgttc
     1861 aatcatcttt agtaaaaaca ggagtacagg tggaaagcaa agaagaatgt ttggatttga
     1921 tgatttccga actagaagcc aattcattag ttactgaaga gtttcgtgct tctatcttct
     1981 ctagagagaa aatggggaat actaatttag aaagtggtgt tgctttaccg catgcgaatc
     2041 catcaatggt cattgaatcc aacattttta ttgtagcatt aaaaaatgcg attaattggg
     2101 gcgatcgaaa agtcaaatta gtcattatgg ttagtttagc tgaagaagat gtatctgaga
     2161 ttagaggagt atatgaagaa ctttaccaat ttattgggca aaaagaaatg gtcgatcgct
     2221 tgttacaagt tcatgatagc gaaaaattac taagaatttt tcaacaaaaa ggagaataaa
     2281 aatgtatcta gataaagaat tatgtttatt tcaattagaa gtgaaagatc aaaaagaatt
     2341 gtttcaagta atgtctgaac aattaaaaaa cgctggttgc gtgaaagata gtttcttaga
     2401 aggtatcacc aaccgtgaac aagaatttcc aactggatta gaagtaaatc aaattgggtt
     2461 tgctatccca catacagatt ccgcacatgt aaatagctct caaatctgtt ttgcctcatt
     2521 aaaggaacca ctggtatttt cagatatgac agataaatct catgaaatac ctgtacgttt
     2581 agtctttatg ttggcaatgt cacaacctca tgaacaaatt gatacactac aaaacttagt
     2641 tagcctattt caaaatgaag aaaaagtaaa tgaattactt gcatgtacca cgaaagaagc
     2701 atttattgag ttagtacatt ctgcaggagt ttattaggag gaatagagag atggatattg
     2761 tattaaatgc ctttgaatgg tttattggat tagggtcaaa cgtcttttta ccactgatta
     2821 tttttgtaat cgggatatta tttggattaa aacctggtaa agcatttgtt tccggcgtta
     2881 ctgttggtat cggttctatt ggtttaggat tagttttaag tttattatca gatggtttag
     2941 gaacagcaat tcaacaaatg ggggaacaat acggtggtgg cctgaatatc ctagatattg
     3001 gcgtaggtgt tggtggacct ttggcttact ctacatcatt gggaatttta atgattccaa
     3061 tttcattaat tcttaacttc atatttgtgg ttttgggttg gacaaaaaca ttgaatgttg
     3121 atatttggaa cttttggttc gcaattttcc taggtatgat tactcaatct gttacgggaa
     3181 acttttggtt tggcttaatt ggcgcgattg tcgcagtaat tttacaatgg ttcttagcag
     3241 atcgtacaca aaaaactgtt agcgaatttt tcggctatcc aggaatctct atcacacata
     3301 tgatgggatt atcaggagta ctatttgcca aaccagtaaa ttgggttttt gatcgtattc
     3361 ctggatttaa taaaatagat gcagatgcag aaacattaac aaaacgtttc ggtgttttag
     3421 gtgacacagt tgttattggc ctaattatcg gtattgtagt gggtattatg gcaggctatg
     3481 acgtagcagg aattggaaac ttaggaatta tgactgcggc agtgatgaag attatgccta
     3541 aaatggtagc aatgtttatg gaaggtttaa tgccaattgc agaagcggcg aaagaattta
     3601 cgaataaaaa gttaaatggt cgccaagtaa acattggaat ggatgcagcg ttaactgtgg
     3661 gtcatccaac tattatgtca acttctctat tgcttgtacc aatctcatta ttgctagcac
     3721 taattcttcc aggaaataaa gtattacctt ttggagattt agcattcttt gctttcgcca
     3781 tctgtttaat gattccttat tttaaaggaa atattgtgag atcattagta ggatgttcgt
     3841 tgtatgttgt tacaacatta tatatggcaa catggttggc accaattatc actgatgtgt
     3901 ttacattagc aagtttcgat gtggggacag caggattagt aactatgttg ttatgtggat
     3961 tatggccagt agggttgttt gttttattag catcttactt tggtgtaatt ggaataggtg
     4021 gattaggagt tgttttagta ggtgcttcta tctacatgaa caaagtgaag aaagcttaat
     4081 cataaaatta aaataagaaa gaagagatca atatgaaaaa attattagtt atttgtggag
     4141 caggtcatgc tacttctaca attgcagtag caaaattatc aaaatggttg gaagaagaag
     4201 gattaacaga tcaagtgaaa atttatcaat ctaaaattgc tgatgagcta aataaaattg
     4261 atgaatatga tgctgtagta agtacaacaa ttgtccctga tgtaattaaa gctaaagtaa
     4321 cgaatggctt gggcttatta acaggtattg gtacagatgc catttatgca gatgtaaaag
     4381 aaagattagg tgtttaatat gcgttttcat catgacactt tgactattga aacagtttca
     4441 gggcgtcctt cgtatcatga tattcgaaaa gaacttttag atacagttga tacagcaggt
     4501 attgagaatg gaatgttagt aatttcgagc ccgcatacaa cttgctcact cttttttgaa
     4561 gaaacgatgc atgacacaaa cttttttggt gatgattatt tacatgtaga catcaacaat
     4621 gtaatggaaa cgattattcc aaaaatgacg agtgaaaatc aatacaacag tccggggcca
     4681 aaacacattg attttggaac aagattatct gatcccaatt accctgcaga aaaatgggtc
     4741 atgctaaata ccgatgcaca tttacgttca tctatttatg gaaccaactc aatgacttta
     4801 attgttcaag aaggtaaact tttattagga gaaatgggcc gtgtctattt tgtcgattgg
     4861 gatcaactaa gagagcgaac tagaacaatc aatctcatga ttatgggaga ataaacgggg
     4921 gaatagagaa tgacaattgc tccatcattg ttaaatgcac ctatcgcaga agttaaagag
     4981 ttatttgaaa tcttaaaaga aaagaatgtt gattatttgc atattgatgt aatggatggc
     5041 tgttttgtac cagatatggc ttttggacca agtgttatta aagatattcg tccactaaca
     5101 gacgttgtgt ttgatgttca tttgatgatt gatacaccag aaaaaattgt cacaagattt
     5161 gtagaagcag gagcagatat tgttaccttc cacattgaag ctaccaaaca cgcaatgaag
     5221 gtcattcagt tagtcaagga agcaggagca aaagtaggga ttacttttaa tcctgcaaca
     5281 ccagtcagtt ttattgaacc agtattatct ttagtcgatc aagtattagt aatgacaatc
     5341 aatccaggaa cagagaataa gcattttatt caagagacag tagttaaaat cgaacagtta
     5401 gatgtaatac gcaaacaaaa tgattatacg tatgatattg aagtagatgg caagattgat
     5461 aatcaaacga ttaaagtttg ttctaaagca ggggcggata tttttgtatc aggcggtttc
     5521 atcttcaatg ggaaaaatac cccgcaagaa caaattgata aactaaatca atcattagag
     5581 gaagtgaagt aaaagatcat gaccaataaa gaccaaaata aaaactattt agaagcgatt
     5641 atcttagaat tacaaaataa ctcaaaacgt attaattctg tagagttaga acaatttagt
     5701 gagaaaattg ttcaagctaa acacatcttt ctagctggtg caggacgctc tggaattgcc
     5761 atgcaagcat ttacgaatcg cttaatgcat ttaggttttt cagtcagcct tgtcggtgaa
     5821 attagttcac cacattcgca acctggagat ttattaatta tctgttctgg ctcaggtgaa
     5881 acaggtagtt taaaaaattt agcggccaaa gcaaatcaaa gtggtgtaag cattgcttta
     5941 gttaccatga aaaaagactc gtcgattggt caattagcag attctgttct tgtattacca
     6001 ggtattacca aagatgaaaa tcaacgagaa gaagatactt tttcacaacc tatgggttca
     6061 agttttgaac aattagcatt tctcaccttt gatagtttag tgttatcttt aatggataaa
     6121 acaggtgaaa gctcagaaac gatgtttgct cgacatgcag attttgagta gtagatttta
     6181 aagataaaaa accattctct aaatgctaat tattcagcaa atatgagaat ggttttttta
     6241 tttgattaga cataacttaa taattcagta gttttgagcg taagcgtgtc atagaatggt
     6301 acctaaacac aaatagcccg atttgataca ctcaaatcag gttatttgtg gcttatatta
     6361 tttacagatt tcttgtatct gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt
     6421 catcgaagac agattgggaa tctctgtaaa aagatgattt aaatatttgc ggatgtttaa
     6481 cccattcgca acagctgtct ggataatact caatataatt ccatttgatc tagcgccttt
     6541 taagctttta gaaaatagcc agttttttcg accgatcact aactctttga tggcacgctc
     6601 tgctcggtta tttgaaagtt ccaagcgtcc atcaagcaac acaatctgaa gtccgtcttt
     6661 atgattgact gcgtattgaa ccgcctttcc aagctgcgat tttggcaaat aagcaaaact
     6721 ctcacaccaa tgaaaaaatt cagtcatcac aggctgcaac gtttccttcc tcgctacaaa
     6781 cctttcttct gcgctaagct tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa
     6841 gtctaatcct attttggcat gactttttgg ggacggttgt ttacccaatg cttcaaaaaa
     6901 ctttctgcga acatgcgccc aacaataaac attgtttacc ggcagctttc cataggccgc
     6961 gtaaccatca gtatgcaaga aaccgctaaa tgattctaaa aaactttttg gctcatcaac
     7021 ggaacgcgaa tttgcgtgct cgtagagcac aacttgtttt tcttcgcttt tacctgaagc
     7081 atataaccag taaaaggtct tttctttatg agtattcaat acccgatagc ttgtctcatc
     7141 cgcgtgtaag acctcctgat tgcttaattc ttgatgaagg caattgtaga ttggctctaa
     7201 aaccatttca cttaccttga tatgccaatt agtgattttt ttacgatcaa gagggaaacc
     7261 aagtgcatgc cattcttttt cttgtcgata tgccggcacc tttttaacaa atttttccca
     7321 aatactctgt gtcacaattg aagaggaacc tagactatta ttgatcaacg gtttaggaac
     7381 aggcgctttt ttgatttttg tttctcctaa tgcttcacat tctctgcaat gataggcatg
     7441 ttggtagatg acttgttttt ctatatgggc aggaacatac aatggctctt ctctaagcct
     7501 tgtcgcaccg atatcatgta aagtatgacc acagtcttca cacaaacaat cggcaccttt
     7561 taattcataa tgaataggaa cttcaggcag accttctaat gaaagtgctt tgagtccctt
     7621 gcgttttctg cgtttaaaag aagataccgt aatgggttcc tggctttctt gtccagtgtg
     7681 ctctggttca ttaaaagttg agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt
     7741 ttcttttttt cgtccaaaca agcgtcgatt catttcagca atttgttctc taagaagagt
     7801 attttcctgt tgctggattt caagcaactg tgttaattgc ttgatttgta caagaagttc
     7861 agattccatt tttgtcattt ttacaccacc attctagcga ttttcttaca tctattatac
     7921 cattttatga caacgaaaaa cagacaaaat ccttgattta acaaggtttt cgtctaatat
     7981 atcgcacctt ttttcgtttc ttttagcgta catttttctt cgattgaaag gccagtcagt
     8041 aagacttctt ttctggaacg cggccattga aggtacccac tttcaaatct tttgtacatt
     8101 aatacaaatc cattttgatc ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa
     8161 aataagaaaa gcgcttgatc gtaaacatca aggtcatatt cttctataac gaaagctgct
     8221 agtccatcaa ttccttttcg aagatctgtt tttccacaaa cgataaaaat atgtttcaat
     8281 gcagttgcat caatgagcat tggcattcaa ctcttttaac aaggtataca aaagatattt
     8341 atccattcca ttataaataa atatttcctt atcttccgat ttgattctgc aagctagttt
     8401 tggcttggtg tcaatttgcg atttttgagc cgcacttgaa aattgaactg gtacaatatc
     8461 ttttttattc attcgttttt ctccttctat atttgatagt ttaattatag aagaaagata
     8521 atcggcgtac gagatactat cctatgacac gcttacattt aatttaatat tttgcctcaa
     8581 attagccaca ctttttatag aactaagagg aaaaatgaag ttttcttcct attaatatag
     8641 ttgtttgaaa tcttaaatat agcgttttac gatgtaatgg gaagtatggt gaataaatca
     8701 actatgttcg tacgaagcat ttactcaact aatttaatcg aatctttcaa taaacaaatc
     8761 aagaaataca gccaccgcaa ggaacagttc caaaatgaag agtcgatgga acgtttctta
     8821 gtctcgtctt ttgatactta caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa
     8881 caggccgaag gcgagctcga acaaatgctg agtcaaccga tggagaatta gaaacaatct
     8941 acagtaggga agaaccattg acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat
     9001 aatgatgaaa tcatttagtt cttataaatt agaaaagaga aaataaatat tccatatcta
     9061 ctttattgga tccgtaacat gactaaattc tgaatacttt ttaaagctta tcatttggta
     9121 aaactgaata taagttaatt atatagactg aaaattttga attttcagaa tagaataact
     9181 taaaagtact gacaaaattt tttaaagggg caatattaat gttctgagat aatcaaatcc
     9241 taccgaaaat atcaaaaatc caatgtgaca caatcagatt gtgtctcatt cgagggaatc
     9301 acacaatccc acgtggctct aggcagaatt gaatttttgc ttggggcttt ttccttatat
     9361 tgattttgaa tgagacttat tatatgttat aatgcaatta agtatcattg gagagggcga
     9421 agaaaatgaa gacggtagaa cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa
     9481 tcttggcttc gggaaaatat ggccaacgaa atttggttct tttttcgatt gggatcaaca
     9541 ccgcctatcg catttctgat ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac
     9601 gcggtcgcgt ggtggtcaaa gatcgattag cgatgaagga acaaaaaaca gctaaacata
     9661 attcggtcta tatttccaac aaattgcgaa aaacgatttt ggagtacgtt caacaggaat
     9721 ttcctgatca actccttcag caagattttg acggttattt gttccctagt cgaaaaggga
     9781 cgaatgaacc gctcaaccgc caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa
     9841 tcggattgaa agacattggc ccacattcca tgcgaaaaac gttcggttac tttctctata
     9901 aacaggggac caaaacagaa atcattcagt ccttgttgaa ccattcttct cagcgcgaaa
     9961 cattgcggta tattggtatt acacaagaag acaaagatac agctgtaaaa agcttagatt
    10021 tataatataa aagttaagtt taaacatgga tagttttaaa ttagtagatt gagtgttggg
    10081 aggaaagaat gagtacatta tatattatag gaaacggttt tgatttaagt catggtttaa
    10141 aaactagata cactgatttt tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata
    10201 aaatagacac aatgataaat gaaaataatg aggtgagaag tacagctgat gataagatag
    10261 gttggtttaa ttttgaaagt agtttgcatg agttaattca aagtccagtc tttgaagatt
    10321 atgtagaaga gtctaaggat tatctcatag gttatggaca tgaagattgg tcagattctg
    10381 cccatcatga ttttcaaaat atggtttatg attatgttgt ttctctagaa gaattacaac
    10441 aacttttcaa ggaatggata cgcaaagaaa tacagccgac aatagaacat aaaattccaa
    10501 aataccaatt ttcgtccgat gtactattct ttacattcaa ttatacagat gttttggaaa
    10561 ttctatacaa gcaaccgaaa gaaaacatat atcatattca tggcagttat gacgatataa
    10621 taatcggcca ctccgagaat tttgacaact ttataggaga tccggttctg ttgcaaagtt
    10681 ttaaataaag aataaaatcc cttacggtat ctatgattta agctgggatt cccaataata
    10741 ccttgatttc agtacagacc gaaaacccga agagagtgcc ttcttttcgg gttttcttat
    10801 ataatcctcg aatggcttcc atgcctttaa tcgtggtaga ggcagtgcgt aaacttcgat
    10861 agaatttatt gcgtctcttt actggacgat ggtcttgttc aatcaaatta ttcaggtatt
    10921 taatggtacg atgttctgtc ccttgataaa agccgccgta ttcttttagt ttcttaaagg
    10981 cacttgtaat agagggggct ttatctgtga ctacaacctt cggttcatca aactgcttca
    11041 ctaaccgctt aagaaaagca tacgctgctt gtgtctcccg ttttttacgt aaccaaatat
    11101 ccaaggttaa accatctgca tcgatggctc gatacaaata atgccatttt ccatgaataa
    11161 aaggattttt tatttttctt tttccaaatt tgatagagta gtttgccata ttcttgcacc
    11221 caacgataaa tcgtcgtatg agaaacgtta atgccacgat catataagat ttcttgaact
    11281 tcacgatagc taaggttata acgaagatag tagcccacgg ctacaataat cacatcctgc
    11341 tgaaattgct ttcctttaaa atgattcatc gtcattcctc ctgctatctt tttctattat
    11401 tctaccttat ttgatagtag atttaaaact ttgcaacaga accggaagaa gcaaagatat
    11461 atttgttaaa aaggtccaaa aaaatatccg cacccccttt tttttgtaaa aaactattgc
    11521 acaatacaag tggcctacgg ctatcgaaat ttccataact ttcgagcaag aatttatctc
    11581 attcaaggct tgatcttcca agtaaaacaa aaaccagtta agcactctgc ctaactggtt
    11641 atgactaaat tttcagttgt atctttctct accaacacta aatgtcgaag agcctttttt
    11701 cgtttatgaa tgtaagaccg cttctcgagc caatgccaaa caaccgagcg ttccagcgtt
    11761 gtcttctaat gctggggtca cgatatattt gcttaactca ggtgtttcga catacccatt
    11821 gaccaatttt gcaaatgcgt cgtggacgtt ttgcagcata tggcgttgtt tcatcacacc
    11881 accgccaaaa atgatcacat ctggagaaaa catcaaggtt gtattgtagg cacattgagc
    11941 aatataggag gcttcgattt cccaataagg atcatcttct gcaacctctt gtcctttttt
    12001 ccctaagcgg ttttcgatgg cgggtccagc ggccatccct tctaaacagt tgccatgaaa
    12061 tggacagctg ccagcaaagt catcttttgg atgagggacg acgagcatat gccccatttc
    12121 tggatgacta aacccttcga taaagcgccc ttcttgtaag gcaccgccgc caacacccgt
    12181 accgatcgta tagtaaacga cactgctcaa tcctttgcca ctgccgaaga catactcacc
    12241 ataggcagcc gcattgacat ctgttgtcca agaaatcggg atcggaaatt cttttttcat
    12301 ggtaccgatg aaatcaaaat tttgccatgc caatttcggg gtcgaggtaa tgtacccata
    12361 agtggcggaa tcgcgatgga tatcgatagg cccaaatgag ccaatcccaa tccctgctaa
    12421 atctgcttgg tattgtttga agaagtcaat gactaaaggc attgtttcct caggtgtcgt
    12481 tgtcggaaag ctcactcttt ccttgatcgt catttcttca tcgccaatgg cacacacaaa
    12541 ttttgtgccg cctgcttcaa ttgcaccgta tagcatagac atttctccct ttattttaga
    12601 atgaaatcac taaataaatg actatatatc atatttaaat tattttttta tttagtaaca
    12661 tgacaagtaa ataactacta ttttttcttt ttgaattttt atcatttttt aaaattctaa
    12721 taatttttca tacaattgat atatgaaaag attaaataaa atgttattct caaatatttg
    12781 attaatctat attatttgat tttggaaaga aaattccatc tttactcagg gaaatatatt
    12841 tatttgattt caagtaaatc agtaccctca gtgactgtat tttcagctac tatatttact
    12901 tctgaataat ccgaagtatt ggtcacaata atcattgttg tatcatcaag accttctttc
    12961 tcaatcattg aacggtcaaa tgctccaaga atatcgcctt ttctaacttt ttgattagta
    13021 gtaacttttt gtgtaaatcc ttttcctccc attgatacag tatcaatacc aatatgaatt
    13081 agaacttcag caccattatt tgatttaata ccataagcat gtccagtttc aaacgtgagt
    13141 tgaatagtac catctactgg agaatggacc gtgtttccat ttggtttaat ggcaatccct
    13201 tttcccatag cttctgatga aaagactgga tcatttactg aagaaagact aatcacttta
    13261 ccagaaatgg gactaacaat tgtttctgat gaaacttttg tttgtgttga ttcaacatca
    13321 gtagatattg gatctgttgt tgctactttt gaagaattaa ctggtgtttg ttcagcaatt
    13381 tcttctgctt taaagatacc agcagctttt gttttaccgt agaagtaagt aattgcaaaa
    13441 gcaactacaa agctaatcaa ttcacacaat acgtaaaatg gaatagatgt tgcattaatt
    13501 gaaagaaatc ccaagaaacc tgctgaacca agtgaaacag caactacttt aagtaatcct
    13561 gctactgctg tagcaattcc tgaaccaatt aaagcacaaa agaatggaaa gcggtatttt
    13621 aagttaactc cgaacaaggc aggctctgta attccaagaa gtgctgagac acccgcagag
    13681 gaagcaagac tttttgtctt agaattttta gttaagaacc aaatggcaaa tgttgcagca
    13741 ccttgagcaa cgtttgccat agatgcaact acaaagataa agtctccatg tccgatacca
    13801 tttgtccacg ctgaaagtaa ttgagtttca atagctggaa atgattgatg taaaccagtc
    13861 atgactataa gtgaatatgt tcctccaaaa atacccattc caataaatcc agtagtgtta
    13921 tatagccaaa caataatgtc tgtaattcca ttagacaaca ataacattac agggccaata
    13981 actgtgaaag tcaaaaatcc agtaatcatt actgataata atggcgtgaa tgtaaagtca
    14041 atagatgatg gcaatttttt atgaaagaat ttttctaata tagataatat ccaaacagct
    14101 gctaaaactg gaataacttg ataagcatag tttgtttgtg aaacatgata accaaaaata
    14161 ttccaatatt cagttgagcc accaagatct ggtgttgtca tgatcatacc aattgcagca
    14221 ccaagaaatt ggtttgctcc aaaacgttta gcagctgaaa tacctactaa aattggtaag
    14281 aacatgaatg gtgcagcgga catcagttgg atcattgagg aaataccttc aatcgcagga
    14341 tacatttctt caattgattt tggtccaaaa agtccagcag ctgttaaaaa gttttgtaat
    14401 gccattaata aaccacctgc aacaagggca ggtataattg gaacaaaaat atctgataat
    14461 aatttaatta atgccataat ggggttgaat tttttatctt tactagcaat ttgttttaaa
    14521 tcatctgttg aaatttccga aagtccagtt tttttgataa gttcgtcata aacgaaatta
    14581 acatcacctg caccgataat cacttgatat tgtccatcaa ttttaaatgt gcttttcaca
    14641 tctggatttt cgtctaaagc tttttgattc actttcgtgt catcttttag tacgagacga
    14701 agacgtgtag cgcagtgtgc agctgcaata aggttatctt ttccgaccgc ttgtattacg
    14761 tcggaagcta ctttactgta attcattcga atcactcctt ttttattttt acgtgaaaac
    14821 ggttcactga aatgcaaatt tattttagca tatcttaaaa atatgtcaaa cgtttgtcat
    14881 atagtttatt tttttaaatg aaatgattga tttttcaacg gaaaacgttt accatactag
    14941 ttgtaaaaag ataaattgaa ggagtaaaat atgaacttac ctcgagaaat acgatatcgt
    15001 gcttattctg attggactaa agatgagatg gataaaatta aggataatgt aaaacgatca
    15061 ccttggcgtg ctagctatca tatcgaacct aaaacaggat tgttaaacga tcctaatggt
    15121 ttttcttttt ttaacggaaa atatacgctt ttttatcaaa attggccatt tggagctgct
    15181 catgggttga aagaatgggt acacaatgaa tcagatgatt tagttcattt tcatgtaaca
    15241 ggagcagaac ttcttccaga cacaaaacga gatagccacg gcgcttattc cggttctgca
    15301 tatgaaatag aggggaatct attcttactc tatacaggaa acgttcgaaa tgaaaattgg
    15361 gaacgtgata cacttcaaat cggagcatgg atggataaag actacaacat tacgaaaggt
    15421 gagaatgttc ttattcataa acctgctgat gtaactgatc attttcgtga tccacaaatg
    15481 tttaattatg aaggacaatt gtatgcgatt gttggagcac aaaatttaga aaaatcaggc
    15541 tttatcaaac tttacaaagc ggatgacaat aacgtggaaa catgggagga agttggcaac
    15601 cttgattttg gcggcactgg ttcagaatac atgattgagt gtccaaatct agtatttgta
    15661 gacaaaaaac ctgttctcat ttattgtcct caagggttgg ataaatctga gctaaattat
    15721 gggaatatct atccgaatac ttttaagatc tgtcaatcgc ttgacataga aaaagtaaaa
    15781 cttgtgggcg cttcagaaat tcaaaatctt gattatggat ttgaagcgta tgcgactcaa
    15841 ggattcaata ctcctgatgg tcgtgctctg attgtcagtt ggattggact acctgatatc
    15901 gattatccaa ctgataagta tgattatcag ggagcaatga gtcttgtaaa agaactttca
    15961 ataaaagatg gtaaacttta ccaatatcca gtagaagcta ttacatcatt acgtgctagt
    16021 tctgaacaat ttacagcaaa agcagaaaca aataatactt atgagcttga actaactttt
    16081 cctccaaatc agaaatcgga gttacttctt ttctctgata gtaaaggaaa tggacttagc
    16141 ttgattgtag ataccaaaga aggaaaaatt attttagatc gcagcaaagc aggcattcaa
    16201 tatgccactg actttggtac gattcgtgaa tgctcaattg atactaaaga aacaactgct
    16261 aacatttttg tagataactc aatcattgag atttttatta ataaaggaga aaaagtgttt
    16321 actagtcgtg tattccctga aaatcaccaa aatggtattc aaatcaaatc aggcgaacct
    16381 actggaaaat actttgaatt aaaatattaa aatggtagca aaattaactg atgtcgctga
    16441 attagctgga gttagcccaa caaccgtttc acgcgtcatt aataataagg gatatttatc
    16501 agaaaaaaca aaacaaaaag ttcatgaagc tatgaaaact cttggttata aaccaaataa
    16561 tttagcacgt ggacttcaag gaaagtcacc tcagcttatt ggtcttattt ttcctaatat
    16621 tagtaatatc ttttactctg aactcattga gtatctcgaa atagagttat ttaaacatgg
    16681 atataaaacg attatttgca atagtcaaaa tgatcctgct aaagagagag aatacattga
    16741 aatgcttgaa gccaaccaag ttgatggcat aatctcatca agccataatc ttggaattgc
    16801 tgattatgag cgtgtgagag cacctattgt tgcttttgat agaaatttag ctcctaatgt
    16861 ttccattgtt tcttcagata actttgaagg gggaaaactt gcagctaaga ctttacaaaa
    16921 aaatggttgt caaaatacca ttatgattac aggaaatgat aacacggatt ctcccacaga
    16981 attacgtgca ctaggttttt cttttcaaaa tccaaatgga aaaattttca aaattcctaa
    17041 taatctgtct actatccgtc gtgaaatgga aataaagtca actattataa atcacaaacc
    17101 agatggtatt tttgtttctg atgatttaac agccattctc actatgaaaa ttgctcatca
    17161 acttaaactt aatatcccag aagatttaaa aattattgga tacgatggta cctcttttat
    17221 tgagaaattt ttcccacaat tgacaacaat aagacaacca attgatgaaa ttgcaagtct
    17281 tattgttgat attcttctga aaaaaattaa aggcgaaaag acaaataagg attatatttt
    17341 accaatctca atactttctg gaggaagtat ttaacgttaa attaattttt taatgtctat
    17401 aaaattcgtt agaaaataca atttgtagat atccaattga tttgtaaaca aaaaggagtt
    17461 gtcaacttgt agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactccttt
    17521 tttcatgctc taaaatgata tttttacaca actgttacgg ttgttttacg aaattggacg
//
LOCUS       DACDNO010000056        17375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDNO010000056 DACDNO010000000
VERSION     DACDNO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.056"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..863
                     /locus_tag="IWI22_002074"
     CDS             6..863
                     /locus_tag="IWI22_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9169453.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="IWI22_002075"
     CDS             885..1394
                     /locus_tag="IWI22_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9169454.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="IWI22_002076"
     CDS             1431..1832
                     /locus_tag="IWI22_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169455.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="IWI22_002077"
     CDS             1944..2297
                     /locus_tag="IWI22_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169456.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="IWI22_002078"
     CDS             2331..2774
                     /locus_tag="IWI22_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9169457.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="IWI22_002079"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="IWI22_002079"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9169458.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="IWI22_002080"
     CDS             3869..4582
                     /locus_tag="IWI22_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9169459.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            5334..5534
                     /locus_tag="IWI22_002081"
     CDS             5334..5534
                     /locus_tag="IWI22_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9169460.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            6102..6665
                     /locus_tag="IWI22_002082"
     CDS             6102..6665
                     /locus_tag="IWI22_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169461.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(6726..7091)
                     /locus_tag="IWI22_002083"
     CDS             complement(6726..7091)
                     /locus_tag="IWI22_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169462.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(7167..7412)
                     /locus_tag="IWI22_002084"
     CDS             complement(7167..7412)
                     /locus_tag="IWI22_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9169463.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(7482..7736)
                     /locus_tag="IWI22_002085"
     CDS             complement(7482..7736)
                     /locus_tag="IWI22_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169464.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(7900..8223)
                     /locus_tag="IWI22_002086"
     CDS             complement(7900..8223)
                     /locus_tag="IWI22_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169465.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            8410..8483
                     /locus_tag="IWI22_002087"
     tRNA            8410..8483
                     /locus_tag="IWI22_002087"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8444..8446,aa:Ile,seq:cat)
     gene            complement(8868..9365)
                     /locus_tag="IWI22_002088"
     CDS             complement(8868..9365)
                     /locus_tag="IWI22_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9169466.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKAHQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(9405..10214)
                     /locus_tag="IWI22_002089"
     CDS             complement(9405..10214)
                     /locus_tag="IWI22_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9169467.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10548..11405
                     /locus_tag="IWI22_002090"
     CDS             10548..11405
                     /locus_tag="IWI22_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9169468.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            11398..12300
                     /locus_tag="IWI22_002091"
     CDS             11398..12300
                     /locus_tag="IWI22_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9169469.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            12333..12683
                     /locus_tag="IWI22_002092"
     CDS             12333..12683
                     /locus_tag="IWI22_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9169470.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            12717..13655
                     /locus_tag="IWI22_002093"
     CDS             12717..13655
                     /locus_tag="IWI22_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9169471.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            13733..14626
                     /locus_tag="IWI22_002094"
     CDS             13733..14626
                     /locus_tag="IWI22_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9169472.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            14664..15227
                     /gene="amaP"
                     /locus_tag="IWI22_002095"
     CDS             14664..15227
                     /gene="amaP"
                     /locus_tag="IWI22_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9169473.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            15240..15428
                     /locus_tag="IWI22_002096"
     CDS             15240..15428
                     /locus_tag="IWI22_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9169474.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            15441..16343
                     /locus_tag="IWI22_002097"
     CDS             15441..16343
                     /locus_tag="IWI22_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9169475.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            16375..16617
                     /locus_tag="IWI22_002098"
     CDS             16375..16617
                     /locus_tag="IWI22_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9169476.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(16873..17244)
                     /locus_tag="IWI22_002099"
     CDS             complement(16873..17244)
                     /locus_tag="IWI22_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169477.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         6118 a         2840 c         3448 g         4969 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     4801 ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat tggtattttc
     4861 tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat attgtatatc
     4921 tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa aggcaacgaa
     4981 cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt ttcatagtta
     5041 agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac aaaaagtgaa
     5101 tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca atgaatttta
     5161 tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa tgagtaatct
     5221 attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt gcctatcaaa
     5281 tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta tttatgaata
     5341 acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact ggtgaagatg
     5401 gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa actttagaag
     5461 aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa gcaacaaaca
     5521 ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa gacggttttt
     5581 ttgtacgaaa aaattcattg gtcgttgaat gggctgaata gtagtgtata aaacaatttt
     5641 gtggagcgtg aagcttttat ggtagaggaa aatacgaata aagtgatgtt gataaaaaga
     5701 ttcctatctt gaatatgaat cgagatgtct tgaaaatgaa tgagtttgac caatcattat
     5761 cctatcgaaa tttacagttt cgttcgattg gtctgtcaat taatgaaaaa gtaaaaaaga
     5821 atatgttcta ggaagagggc ggcacatcta aatttttaag taacggacag ttcaaatatc
     5881 aagcattgaa agaggctatc ctactgtttg aaggaatacg aatatataaa aacgacaaag
     5941 aggaaaaagt agttcattaa atcccttcaa gtgatgaaag aatgagatct aataaaacct
     6001 gatctttcat ctgttttttt aataacgtaa taattttttt attttttgtg ctttcgccgt
     6061 tattgtagag tgatttagca ttcttatttt taaaaataac tgtgttatat attaaaaaag
     6121 gcataatagt tataagcttt tttaaagaat atttagaaat tattaatttt cgtctgaaaa
     6181 caaaaaaaga aagcgttatt attttagtct attttcaggg ggggaacctt atgataaaaa
     6241 aactattttt actatcaata ttaacgctag caatggcagg ttgttctgac tctggcttag
     6301 caactgaact gatcacaaaa tcaactgaag ccacgacaga ggagttaaaa tgtggaaacg
     6361 atcacagtga ctacagaggc ttaaatcgac gatgcatggg aatattaggc agaagcaatt
     6421 gtaagcatcg atgtagtaat cgaagaaggt gtagaagaag caacagaaaa atcaaaagaa
     6481 agatcaaaag aagcaatctg tgctgtgcat cgtatacaga cgtcgaagat gcggtacttt
     6541 taaaccacgt caggagatgt tcatttatgt ctagggaagt tggcgatgaa ccaaaactag
     6601 agtacgcttt tagttttgca cgtgaggatg aagataaccc taaaacttac gacattttag
     6661 cttgatctaa gatggttaaa gctttttaat gaagtaagag gagatagaag gcttttctag
     6721 ttatattagg taagataaat tcctttttgc tccaaatttg ctaaacattc tgctagataa
     6781 acttcatcgt gttccggata aatcggcacg ttgcgctcta cagtaggtaa atcaaaaaat
     6841 gcttcgcatg atttgccacg atacatagga tttttccact ctggtgctgg gctgtaacca
     6901 cgtttttcca tttcatccat gacaagctga tgatattgga ataatctata aggagaatag
     6961 tgaaaaacat aatccacagt agcatgtttc tttccccagc catttcctct taatgcacag
     7021 cattcccgat gttgtcctaa aagctgttga cgaggaagct ttgaaatcaa tttttcatgc
     7081 cataatcgca tagaaatgtc tcctttatat atagattgtt aaccttagac gttaaatgaa
     7141 aaaaccagca ccaaggctgg aaaaaatcac ttcagtttgc ttgctgtttc tgagcgacca
     7201 attaggtaat caacggaaat atcgtaaaag tcagcgatat agattaattc aatgatgctt
     7261 ggaatttctt ctccttcttc gtacttacga tatttgaata aaggaatact taacgcgcta
     7321 gaaacagcta cttgagataa agatcggcca gttcttaaga aattcagacg acaacgtaaa
     7381 gtatccagta aaatagcttc ttcgatactc atttaatcac ctttcattat agtagaatag
     7441 ttataatgac tatagaaagc tacacatgca atcaatagta ctcaatgttt tttatttaaa
     7501 cgtatcaaat aaagaatgac gatcaataaa gacaaaacca gtcctgataa ttcttctata
     7561 aaaacactat cgggttcaaa gtagttgtaa ttaaaaactt tccacataaa gtagatagcc
     7621 ggaagagtca aaatagctaa ataaaaccgt tctttgttcc aattcataag ataaacaata
     7681 cttgcgacca gtgcgaccgc aggcacccaa aacaaaactc tcaccaacgt aatcatgtaa
     7741 cggactcctc aacgattcat ataataaaag ataacatgtt attgcataca tcacaaatga
     7801 aatgctagga aaataaaaga agaatagatc aatgaaaaat gaaaaagcag aacagttttc
     7861 tgctgataag caggaactgt tccgctgaat gtgtcttatc tatcgagaaa aaatcaatgg
     7921 attgatatct aagtaattca atacatataa aaagaggacg atcgcattca ctaaaaagga
     7981 aagaatgatg atcccatagc gaatggaaac atatttaaca aatacttttg tttgaatacg
     8041 ggcatatctt tttcctttat agctgagcat catgacaaga agcaaaaggg taatcacacc
     8101 ttgaaaaatc attccaataa acagaattcc tgaggtttga taatttaatt ggagaaaata
     8161 agctgctgcc gcaactaaat cgaataaaat cgcaataaat ggcaaaataa tcactcctaa
     8221 cattgagaat aaataaaaga ataacatttt ttctaaaata agcaaataaa aattaaagta
     8281 acaaaatagt tatgacacaa gtaaaagaaa taagaataac aatattattt tacttatatt
     8341 ttgaaacttg tgagagtttg tcattcgtgc tatagttgaa aagtaacaaa aataaatttt
     8401 atttatgatg tacctttagc tcagttggtt agagcagacg gctcataacc gtccggtcgt
     8461 aggttcgagt cctacaaggt acataagagg ttgtgaaaaa gctgccctgc atcaagtaat
     8521 aagaacagcc aagtaaaaac agcttcttat gtttttactt ggctgttctt attactgcag
     8581 atgcaagctt ttgaataccg tttaaggaag aggttgtgaa atcagcgttt tgacctgagt
     8641 attagcttaa caaacggaaa aatagttcct catattttgc cgtttgttaa gctaataccg
     8701 agggtcagct gattgaacac catttaagaa agaggtcgtg agcctgccgt ttcactctgt
     8761 gtcataaaaa agccaatcta ctttgataga aaaagcacgt aggactaaaa cagttctgcg
     8821 tgcttttttt attgtgtgtt aaatatcgta aatgaagata gatattttta tgattcgacg
     8881 atagagacgt tgaaatcttt caacagctct aaagcggatt ctccgtgttt tctactggag
     8941 actaacattg gatcaacaaa aaagtctgct tcaggaaaag cactctgcag gcctatcaga
     9001 ttagagagta cacacaagtt tgtttcaatc ccgactactt cgatctcttt ttgttcttct
     9061 ttgctttcaa ataaagtttc ctggatttca gctaaagttt caggtggaat cgtaaagtaa
     9121 tactttttaa tcactcgttc attttcttgt ggagaaagaa gagggatcaa ttgcaaatct
     9181 tctcgttctt cttctttgtc cttcagttca actggtatat cccgtgtgaa gagaatatat
     9241 tcattatttt ggtgtgcttt agctaatctt tgagcaatac gttctgccaa ttgatctgac
     9301 tctggaatat aaaattcgct gtttggatca agaatatgat tttgcatatc aataataact
     9361 aacattttct ttcctcctac attcatatcg tcattttatt ctttttaata gaagtttttt
     9421 aaaatataag caacaccagc ttcttcattc gtcagtgtta cttggtttgc aatttctttt
     9481 acttgtcgct gtgcattccc catcgcaata ctcaacccgc tgtaattcaa catagcaatg
     9541 tcgttttctt gatcgccaaa agccatgatg tcactcgatt cgattccaag gtaatccgcc
     9601 agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg tgatttctaa gaaatgcgga
     9661 tcagagaata ctgattggat accatctgct tgcagatctc tttgtagctt ctgtagtttt
     9721 tcttcactct tatctgttaa tgctaattta agatagctaa gagaaggatc ttccaacata
     9781 tttaaaattt gatcaaaaga atggttctgt aaattgaaat gttcataata agggtcatct
     9841 ttgactagat ttgattcatc gaatgtcagc tgatcatctt gggtttctcg gctagcaatg
     9901 atggatattt tcgcttcttg agcatagaag aaggcttttc tcagtgtatc gctatctatt
     9961 tttgaacgtc tgagaacttt cgccattttt ccatccttca tttcagcaat cagtgaacca
    10021 ttggaagaaa tcaaatagcc accgattcct aattcggaaa aaatatcttt agcagaaatg
    10081 ctgttccttc cggtagcgac ggttacgatt ccgccatttt catcaaattt ccgaagtgct
    10141 tggatatttt cttctggtat ctttccattt cgatcaagca aggtaccgtc taaatcaatc
    10201 gctgctagtt tcattttatc ttcctacttt ctttttacat atctattttc ctgttacctt
    10261 aagattaaat gagaatttaa aaaaatgaaa ctaacttgct ttgtcattat gaattttttc
    10321 aaaaaacatt cttaagaaaa aggtggctat atatagtagt ttttggtcaa tacctttgaa
    10381 taaaaagcca tgaaaaacga atagttagcg cttaccgatg cgttttgtga taaaaaaatt
    10441 ttaaaatatt caatgaaaac aaagcatata ttttgcttta aaaccaaatg aggaatattc
    10501 tagatgtaga acaggttaga aaaagattag aaaaaggagt gattttgatg gagaagagat
    10561 acggcgtagt tcctgtggta actgcttcag acgaaaatta tgctccttac ttgagcgtga
    10621 tgatcgcaac tgcattagaa aattgcaata aaacaagacg cataaaattt tatgtgattg
    10681 acgatggtct atcagaatat agtaaagaag gattggaaga aacggtaaat aaatattcaa
    10741 gtaatgcaag tatccaattc ctgacagtgg aaaaagatat ctacgaagac ttcctagtca
    10801 gtgaccatat cacaactacc gcttacttaa gaatttcttt accaaactta ttagctaaag
    10861 aggattataa gaaagtcctt tacttagatt cagatgtgtt agtactagac gatatcgtaa
    10921 aactatatga tgagccgtta aatggaaaaa caatcggcgc tattatcgat ccaggccaag
    10981 tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt gtacttcaat tcaggagtga
    11041 tggtgattga cattgatcaa tggaataaaa aagaaattac agaaaaaact atccattacc
    11101 tcagtgaaaa tggcgatcga atcatctatc acgaccaaga tgctttgaat gctgtacttt
    11161 atgaagattg ggaacaactt catccaaaat ggaacatgca gacttcgctg atatttgaac
    11221 gtcatccagc accaaatgaa aaatatgaac gtcagtataa agaaggaaat gaaaaacctt
    11281 cgatcgttca ctttacagga catgataaac cttggaatac gttgaaagat catccgtata
    11341 ccaatttata cttaaaaaaa ttagcacaca gtactctaac gaaagtaggc gagatcaatg
    11401 aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa aattcgtacc acatttagct
    11461 gcattatttg tctcagtatt agataattgc aacccgtcta aatttgttcg gttctatgtg
    11521 attgacgatg atatcgattt tgaaagtaaa cagcttttac gcttctcagt gaaaaacgca
    11581 cgaatgaact cagatgtgga attcttgaaa atcaataaag aattctttac aaatgtggta
    11641 atcagcgatc gtattccaga aacagcttat tatcgtattg cgattccgga attgttccgc
    11701 ggcacagaag ttgaacgtat cttatatatg gattgcgaca tgatcgcttt acaagatatc
    11761 tctaaattat ggcgtcttga cttcggtgat tcaatcgtag cagctgttga agacgctggg
    11821 ttccatcaac gactggaaaa aatgaaaatt tcagctaaat caatgcgtta tttcaactca
    11881 ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa atattacgca aaaagtatta
    11941 gatttcatcg aacataatcc agaaaaatta cgattccatg accaagatgc attaaatgct
    12001 atcttacatg accgttggtt accacttcat ccgcgttgga atgcacaagg ctatatcatg
    12061 gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg aatacgaaga aactcgcaac
    12121 aatccttaca ttatccattt ttcaggacat gtgaaaccgt ggagcaagga ttttgaaggg
    12181 ccaacgaaga aatattacga aaaatatgca gggatgacgg ctttccgttg tgtagctaaa
    12241 ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa cagatgtagt tgttaaataa
    12301 tagaagacaa caactgaaga gggggagtag gaatggaaat tacaatgtct aaaaatgcag
    12361 tagagacgtt aattgaaaag gttggggaaa atacaaaaat cgcactcgcg cttatcaatg
    12421 attccgatcc atttctaagg gacaaaggtg cttttgcaaa aggcagcttt tttcaaatca
    12481 ttccttttgt ttcggaattt ggagaatatg cgactaaaat cgagcatcca ttattggaca
    12541 tctatacatc taaattagag caaaattatt tcggtaaacg attgaacatg gacttcaaca
    12601 aacagcttga cagcttttca ttagaaaatg aaatcgcagt actcgattat aatatcaaat
    12661 taaagaactg cttcttcagc taatagtttc gagaaagtcc aaaaaggagg tatacattga
    12721 gatactactt tgtggatgaa gaaaagaatg taatagaaac agatgaaaaa aaatataatt
    12781 ggctcgtact tgatagtgca aagcccgaag agattgagca agcaatcgaa gaattcgagc
    12841 ttcctgatga tattttcgta ggaaccgaat atccggaaga agtttctcgg atggagcgcc
    12901 tttatgagac gaaactagag catccatttt ctttggtagt gattaatcta gatcagtcag
    12961 ataatcgtat cgaaaaaaaa ttaacgccaa tatcttttgt actttccgat aatctcttga
    13021 ttacttgtat cgatagaaaa accgaatttg tggatcggct gctgacaaag catggaaatc
    13081 atttaaactc gtttgaaaag atcatcacgt atacattatt agatatttac acccattacg
    13141 tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt ggataaagca gctcgtaaaa
    13201 caacggaaaa tgaagaactg tataaacagg cagatcttga aagagacatc gtatatatcg
    13261 atcatacctt aagagatcaa cgggaaacaa tggatcaatt atggaattct gaaactttca
    13321 tcaaacgttt agacgatgaa cagctactct atgatgtcca tttgagacag cgacaaacag
    13381 aaaaaatgat cgagatctat cgagatttat tagagagtat tggtgatcta ttcaacggga
    13441 tgatggacaa taatctaaat catatcatga aatatctaga ttcagctgca ttaatcattt
    13501 ccgttccggc gttgttcgca gggatttggg gaatgaatac aggaggatta ccgggtaaaa
    13561 gttctgctct aggctttata ctcgtagtct ttggctcttt gatagcagca gtagttttag
    13621 gttaccattt ataccggaaa gattatacaa attaaagaat ctatttaaaa caggtatcaa
    13681 acaaagaaat ttgttttggc aatacaattt aaaaactagg aggaatacag caatgacaga
    13741 accaaaatta atcgacccgc gaaacttgta ccatacggag aaatttccta aacaagatca
    13801 ggatacacca gctttacagc agaatatgat tcctgttcct gattgtggag aagaatccta
    13861 tgaaggcaat aatcaactag aaaaccgccg tgtactaatc actggtggag attcagggat
    13921 cggtcgtgca gcagcgattg catttgctag agaaggggca gatatcgctc ttcaattctt
    13981 cccaggagaa gaaaaagatg cagaggaagt agcaaaatac gtgaaagatg caggacgtaa
    14041 gattgtctta ctaccttttg atcttcgtga cgaacaagca cctaaagaaa tcatagataa
    14101 agccgtgagt gaacttggcg ggctagatac gatggtacta aacgcaggac agcaaatttc
    14161 ttgtcagtct attgctgagt taccacttga gcaagtcaaa gacacattta tgataaacat
    14221 catcgctatg tttgctttag tgaaagaagc tgttccgcat atcccagcag gaggagcaat
    14281 cgtaacaaca acttctgttc aagcattcaa tccaagcgaa catttattag attacgcagc
    14341 aacaaaagct tcaatcgcta actttacgat cggtttagca aaacagcttg cacctaaagg
    14401 aatccgtgta aatggtgtag cacctggtcc aatctggaca ccattacaac tagacgaagg
    14461 acaaccttct gattcgatcc ctgaatttgg tcagcaaagt ttactagaac gtgcaggtca
    14521 gccaggagaa ttagcaccag tttatgtttt cttagcatcg aataaagcaa gttatgttac
    14581 agcccaaatt tacggagtaa ctggtggaga agcgatcaac ttataagaag ttgatcgttt
    14641 cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga gtgatcggaa
    14701 cattactttt gctatctgtg cttttgtttg cgatactatt aagtgcaacc tatccattgc
    14761 cttttcagct agagaagttt cgattctttt ccatcactaa ttcatacgtg caacaatatg
    14821 tattttggct tgccgttgca tttgctattt tgacaattat cacgttactt gttttcctgt
    14881 tttatccaaa aacaatcgga aactttctac tgaaacatgg tgatggggag ttgacgttgg
    14941 ataaaaaagc aattgaagga atggttcgtt ctcacttgca tgaagatgaa ttcatccatt
    15001 caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta aaaggggagt
    15061 tgaaacgaac ttcagcactc attggtaaaa caggaacatt gatgaaagaa atcgaaaatg
    15121 aagtacaaca catattggga acaaaagagc cagtgaaagt atcggtgaaa tacacaggct
    15181 ttgaagaacc agctaaacga agcaatgacc gcaaacgagt agaatagggg ggaccaaaaa
    15241 tgaaagaact attttcgaca tacaagctcc ctatcatctt tggggtactt ggattgatat
    15301 tagccgtttt atggatcacc gtgggctttt tcaagacctt attgatcttg atcatggcag
    15361 cgatcggcgt cgctatcggt ttttatctga aacaaacgaa aatcttagat cactatttta
    15421 aatcttagga ggaaacatcc atgaataaca ataacaaaaa taataacaat aaaatgaaca
    15481 accaaaaacc agtgtctgca catccagacc caacgacacc aaaccaatca aatggtgtga
    15541 acgatgaaaa gaacaaaaca gcaccagtat ctgcacatcc agatccagta gtccctaacg
    15601 ttcaacaagg agtagaagag aagaaacatg atgcagaaaa gcatgcacct catcatccag
    15661 gaactccaga atctaaagtt ccagaacaac atgatgcaat cgatccttct aaacctgtgg
    15721 aagagcaaaa acatccttct gaccatgcga aagcacaaga aaaacttagt tctgaccaca
    15781 caaacggaca aggaaaactt ggttctgatc atacaaataa ccaaggaaag catgattctg
    15841 aacaggaaga ttcaatgcgc ggtgagctga attatgaaga taaagttgtg caaaaaatca
    15901 tcggaatcgc aattgaacaa gtagacggtt tattatctgc aaacggtggt ttcttctcta
    15961 atgtggcagg aaaacttgtg aatacagata atgtaacggc tgggatcgaa acagaagtcg
    16021 gtaaaaaaca agtagcagtt gatatggatg tcattgtgga atatggcaaa gatatcgaaa
    16081 aaatctttga agaaatgcaa gaagtaatcg gcaaagaagt aaaaaatatg acacatttag
    16141 aagtgatcga agtcaacgct aacgtagtgg acatcaaaac aaaagaagaa tttgaaaaag
    16201 atcaagaaac ggtgcaagac aaagtaactg acgcagcgaa aaaaaccggt gaatttgctt
    16261 cgaatcaaac agataaagta aaatcagcag ctggaagcgg cgcacaaaaa gtaaaagaaa
    16321 atacagaacc acgggttcag taataaggaa gtaaaacatt aggaggaacg aaacatggga
    16381 tttatttggt cacttatcgt cggaggaatt atcggagcaa ttgcaggagc aatcacgaat
    16441 aaaggttctt cgatggggat tattgcaaat gtcgtagcag gtttagttgg atcagcactt
    16501 ggtcaagcat tattaggttc ttggggacca agtattgcag gaatggcaat cgtgccatca
    16561 ttgattggtg ccatcatttt agtactaatc gtatcattct ttgctcgaaa actgtaaaaa
    16621 gacttttaaa aagaggtcgt gaaaaagctg ttcttcatca agtaataaga acagccaaac
    16681 aaaaatagct tctcatattt ttcatttggt tgggcatatt atcgaagatg caagcttttg
    16741 aacactgttt atacggaata agaggctgtg acaagacttt tgtcacagcc tcttatttat
    16801 gcgggcaaat tcccaattag atttttaaaa gtttttcctt aaatgtgtct ttattgatac
    16861 agagaagctt ctttaataaa acaatgcctc cctaaatttt cttgagatag gaaaggtgaa
    16921 catacaaaat aatacgacca ctttgaaaac ccagatgatc cacaaatgga tcgtatgata
    16981 ctgatgaagt aaaaagcaat ggatcagaaa ctcaaagaga ttgaaaaaga caaggaagaa
    17041 ataatatctc ttttttactc ttgtctgttt tagccaataa tagctcgtaa agtaatctag
    17101 acaaacaaat agaagtaaac aaagtttcgt aagagcagca atcgtatcat gttgctctgc
    17161 tagacgattg acgagcagga aaaatgttgg taatggaata tagatcacga gtaatgaaag
    17221 aaaaaagtaa acaataaatg gcatactatt cctcctaatt gtgtgagttc gccctcattt
    17281 taaaataacg atatttgttt gtgaagcttt ttcgtgtcgt aacaaatgtt atctaatgta
    17341 tttttaaata aataatcatt attatcaaaa taatg
//
LOCUS       DACDNO010000057        17328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDNO010000057 DACDNO010000000
VERSION     DACDNO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.057"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..946)
                     /locus_tag="IWI22_002100"
                     /pseudo
     CDS             complement(1..946)
                     /locus_tag="IWI22_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1147..2421
                     /locus_tag="IWI22_002101"
     CDS             1147..2421
                     /locus_tag="IWI22_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9169478.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(2804..3532)
                     /locus_tag="IWI22_002102"
     CDS             complement(2804..3532)
                     /locus_tag="IWI22_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9169479.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(3510..5084)
                     /locus_tag="IWI22_002103"
     CDS             complement(3510..5084)
                     /locus_tag="IWI22_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9169480.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            5313..6251
                     /locus_tag="IWI22_002104"
     CDS             5313..6251
                     /locus_tag="IWI22_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9169481.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            6263..7174
                     /locus_tag="IWI22_002105"
     CDS             6263..7174
                     /locus_tag="IWI22_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9169482.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            7198..8670
                     /locus_tag="IWI22_002106"
     CDS             7198..8670
                     /locus_tag="IWI22_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9169483.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            8721..9332
                     /locus_tag="IWI22_002107"
     CDS             8721..9332
                     /locus_tag="IWI22_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9169484.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            9362..11275
                     /locus_tag="IWI22_002108"
     CDS             9362..11275
                     /locus_tag="IWI22_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP9169485.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            11403..14384
                     /locus_tag="IWI22_002109"
     CDS             11403..14384
                     /locus_tag="IWI22_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP9169486.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            14381..16042
                     /locus_tag="IWI22_002110"
     CDS             14381..16042
                     /locus_tag="IWI22_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP9169487.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            16082..17014
                     /locus_tag="IWI22_002111"
     CDS             16082..17014
                     /locus_tag="IWI22_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9169488.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         5585 a         2844 c         3462 g         5437 t
ORIGIN      
        1 cttcgactaa ttcatgaaaa aagggatacg cctcctttag aggcgcagaa aactcaagca
       61 atcgatcaat catcattgct tcagtgagaa atgggtattt tggtgctcgg aaacttttcc
      121 atgttttgta ctcataatgg ttgatgcttg cacgattttt tagcagaaag cgccagttct
      181 ttttcagttt ttctgcctga cttttctgtc ctgctttacg aagctcattc atttcacgga
      241 tacgcaactc attgagtgcc tgattcatgt gtttgacaat atgaaaccga tcaatcacga
      301 ctttcgcatt tggcagaaca cgtttggtaa gctggaagta agcggcgttc atgtctgtga
      361 ctaagaattc tacttcttct ggattggtgc agcctaagaa atagcttgtt aatcgaggta
      421 atttacgcgt aggcaagaca gcaattaagt ttcctgtttc accatctgcg caaataaagc
      481 tcattttatc ttctatggaa gcgtgcgaac gaaattcatc aaccatcaat actctgggga
      541 gaatcttctt agaaggcttt ggtaaatagc ttttaaactc tttcaatgta cgagtaacgg
      601 tagtcaaaga tacctgacaa cttttcgcaa taaaagacaa agatactttt tcagtcagta
      661 aagaagcaat tttatatcga acatgatctg cgattgaatg gctgagtcgg acaaaataac
      721 tttgagccgt ccaatgggta cgacagtttt tacaggtata gcgttgcttt tttaggcgcg
      781 taaccaaagg catatgattg tattgttcaa aacggacaat cgtttccttc ttcccatttt
      841 tcactataat tgctttcccg tttccatcta ccacagtaga accacaactt ctacaagcac
      901 gaggagtagg cgagagaaca gcatcgacga ccaacgtctt tttcttggct ctttgtcaaa
      961 taggactgat agagtgatat aagaacatct aaggtagaat tgattttgct tctataatat
     1021 tttgtgttgg tggaaaatcg aaggcgattt tctactaaca ctttttcttc gtttcaactc
     1081 aaaaagagac tcacttttgg taaaatttag tcgaccaaaa cgtcatccta ccaaaggagt
     1141 ctctcaatgg ataatcatac tagaaaatta ctcggattaa cagataaaca tttatttttt
     1201 gatgaagaat ggctgattga aaaagaatat aaaggcgttc aagcgcagtt tattaaagga
     1261 aaattggatg actctccttc ctattgtgaa cactgtcgat cggttcgtat tatccgcaac
     1321 ggttcctata cgacacgcac tcagatattg aaagtcaaag aaaagttaac tattttagaa
     1381 ttaaaacgta cccgttttct ctgccatgat tgtggaagga ccttttctgc gaaaacggac
     1441 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcaattct catggaactg
     1501 tacgaaaatc aatcacgtaa actaatcgct aagaagtact tcgtctcgga tgggacggtg
     1561 acacggatct tgcgtgaagc aacgaagcat taccagtcac gtatgaactt ccttccgacg
     1621 gtgttgtgta tggatgagtt caaatcaatg aagtctgttt ctggttccat gagttttatt
     1681 tgtgttgatg ggacaaccca tcagctattt acgatccttg aagatcgcag actttacaaa
     1741 ctaactcaat acttcctgca ctttcctaga aaagcccgtt taaaggtcaa gtacctagtg
     1801 atggacatga atgccagcta ttgtcagttg cttaaaacag tctttccaaa tgcggagatc
     1861 gtaactgacc gctttcatat tgtccaacac atcaatcgca gcttcaatca attacgtgtc
     1921 cagatcatga atcgttttcg aacctcacac tctgaaaatc agaagaagta ccgtcggctc
     1981 aagcgatatt ggaaactctt actcaaagat tccacgacat gggagccact caaacggcat
     2041 tatcatcgac tgtttaaacg accaattagt caaacagaaa tcgttgatga gctgttaagc
     2101 tacaacgaag aattgcgaac tgcgtatcat ttttgtcaat tattacgcta ttatttcgtt
     2161 aaacgaggta cggaaggctt tcaagaaacc cttaaaacaa tttcatctac gttacctcag
     2221 tcgtttaaaa agaaattcac gatcttccat tgctaccaat cgggtatttc aaatgccttt
     2281 aaggtgtccc actcaaacgg cgtcactgag gggctgaata acaagatcaa gctgattaaa
     2341 cgaattgcgt ttggttatcg caacttttat aactttcggg cacggatcta tttacaacaa
     2401 ggtctgatct ttgaaaacta aaaaaatatg cggcgcacag aacgcgccgc atatcggact
     2461 aagttttatt caaatttgtc ttcaccaaca cgatttgacg aagagcctat ttaaataact
     2521 acatccatct attctatctt ctaaaatagc ctttttcatt tcagttaaat agttcagcgc
     2581 catttttgca ctcgacttgc atagtagttt tttccatatt cctcaacctc agcgggggtt
     2641 gaagacataa ggaaaatcct atgcgttatg ctcaactgga tctgattaaa tattctaatg
     2701 tatttttctc taccatcatt aaatggttaa tagccaaaaa tcaccacttt tcaatggctc
     2761 cttgttgtat aagtataact agaagatgaa cacgtctgag aaattataga agctttttac
     2821 ggtaattact ggggctgtgt ccagtagttt ttttgaagag aacagaaaaa tagctcacgt
     2881 gatctattcc tactttttga ctaatttcat caatagataa gaatgtgtct ttcagtagag
     2941 cacttgctct tttgattcga aagtctagca agtaatcatg aaaggaataa cctgtttctt
     3001 ttttgaaggc ccgtcctaaa taagcgggat ggtaatggaa ttgtgtagct acatctttga
     3061 gcgtaagctt ttcactataa ttgttttgaa cgtaatcaat cacttgttga attgactcat
     3121 taaaagaatc atcagttaga gagttttgat tagctgctga tttacgcatt ttttcttctt
     3181 gtactaactt aactaaataa ggaaaatcaa tgggtttcgt tatgtaactt ttaatagata
     3241 aatcaatccc cttttttgca tactgaaaat tttggtgggc actaatgaca atactagtaa
     3301 agtagcgatt ggatgtttgt tgcaaagatt cgattaattc gagtccagaa attttcccca
     3361 tcgtaatatc tgtgataagt aaatcaaagc gctggttttg caggttttta aatgcttcgt
     3421 ctccgtctgt gtagcagtga tcgataatga tgtcggggac tttcttcaaa agataatgcc
     3481 ttatgccttc tacaattgta caatcatcgt caacaattgc aattctcaac atgcatattc
     3541 cccccttttt gtattttgat agtaatcgtt gttccgacgt gtagttggct ttctataaca
     3601 aaacgtgtat ctccaggata gagatgcttt atacgtcgaa aagaatttga gatgccgata
     3661 ttctcactat aaggaatatc atcattaagt ttttgttgaa tatgctccag cttgtcttgg
     3721 tccattcctt tcccattttc aacgattttt aacacataat ggttggttgt ttcagttgca
     3781 tagacagtca aacgattttc cttttttgat gaatcgaagc cgtgtttaat ataattttca
     3841 acaaatggct gtaaagaaag ttttggtaac attaattgat caatatgatc aatcagctga
     3901 tagtcaaagt tatgtcgata acgacgttga tgtagttgca aggagaggtc gatattaatt
     3961 aattcctctt tcaaatcaat tatgtcgggc tgatatgtat tattgcgata caacttgctc
     4021 aataaatata tactgtcgct gatttcttcg tcatcattca atatggcagt cattcgaata
     4081 aattccaaat tattaaatag aaaatgaggc tcgatttgat attgaagtgc tgcatacttt
     4141 gcttcttgct tcaaccaatt attttcataa agtgtttttt gatatgtagc attatctaaa
     4201 taaaactgat tgatatgcat agcgatttct ttcaaatcac cgactttgtt ttcgatattg
     4261 attgacttgt agtctcctgt agatgaatga tttaaggaag taatgatttc ctgatactgc
     4321 tggatatagt tacgaaaact tctatttaat aattgatgaa tagcaagaat aaacaaactc
     4381 attaataaga aaatcatgat tgccagacct aataaggttt gtgacgaata atttggattt
     4441 tcatatactt gtaatgtgtg gttaccgata gctatctgtt gcgttttttt ccatttagaa
     4501 ggcgagatgt cttcagataa attgacggaa tcaataccat tagacaaaga ataattgtaa
     4561 tgtgttggaa agagactctt ttttagcgcg ttgatcgaaa cgtctagaat caatgtagca
     4621 tcgggcatgt cctcactaat gaaacttaat gaacgaataa aagaaatttt ttcgtttttt
     4681 gtaggtgcgt aggcacttaa gtatttttcg taatctaatg agatagatat atttttaact
     4741 tcatcatctt gcacaagttt ttttaagtta tctactaaat aggtagggga gcgatccttt
     4801 gacaaaatat cttccatgta ttcagcaggt gatgagaggt agtagtcatt aaggttttct
     4861 tggaaatcat aattttgata gatctctgtc acgataccgg agagaatacg attatatgtt
     4921 tccatatagt gggtgatttg ctgaatgttt tgcaggtttt gatttttttc tttttcaatc
     4981 acattgatat aggaaaagaa caatgtgaaa caggcaagta aagtaataaa aaaaatcaat
     5041 atgtatgtaa aacgcataat aacttttgat aaatcgtttt tcattcaaag tcctcatttt
     5101 gataatctct aattttatga atcattatag cacatattac aagtgatgta aacgctttgc
     5161 aaacagggct acttatgatt gtgaatacag tttccgttta aaaactattc tattgtagag
     5221 gtttaataaa ctaaaaataa tgtataaaaa attaatgtaa gcgattaacc aagtgggtat
     5281 aatcaaggaa acgctgagag aggagagaca aaatgaagct aaagaaaggg ttattaggaa
     5341 atctaaaaaa aaaccgagca ttgtttatta tgcttttacc aggtgcgtta tggttattta
     5401 tattttgcta tataccaatg tttggagttg taatcgcttt taaagatttc agaatttcat
     5461 caaggggatt ctttacaagt ttgttcaaca gtccttgggt tggctgggaa aatttcaaat
     5521 atctctttgc ttcgcaagat gcttggatca ttactcgcaa tacggttgtt tataacgttg
     5581 taggtatttt gctaagtact ttttgtacag tgtcattagc aatcattctg agccaaatgg
     5641 cgaacaaacg gttattgaaa aagatgcaga catttatgat ttttcctaat tttttatcat
     5701 gggtagtcat cagttacttt gtttacgcat ttttgagtac ggataaagga ttaattaata
     5761 ttttactaag caattttggt gtggaaccca taaattggta tagtgaaccg aagtactggc
     5821 cttttattat tgtttttacg agtttatgga aaggcgtagg ctatggatcc atcatttatt
     5881 acgcgtcaat tatggggatc gatactagtt attatgaagc tgctttagtt gatggtgcca
     5941 gtaaatggca acagataagg aatgtagtct tgccacattt aagatcgatt attgtcatta
     6001 tgttgatttt gtctgttggt agtatttttc gagctgattt tggattgttt tatcaattgc
     6061 caagaaattc gggggctctt tatgatgtga cgaatgtatt agatacttat gtctaccgtg
     6121 gattaatgaa tagcggagat atcggaatgt ctacggcagc gggattatat cagtcatcag
     6181 ttggctttgt cttgattttg attactaata agatagtcaa caagttagat cctagttcag
     6241 cactatttta ggaggaatca aaatgatatc aaaattaaaa cggcagatgt acttttcgct
     6301 gccagtcgaa cggtttttca caatattact aatcttattt actcttagct gtatttttcc
     6361 ctttattttc gtaatcgtca tttcattaac agatgaaaaa gcattacaac tcaacggtta
     6421 cgaattattg ccggctcaat ggagcactga tgcatatcaa tacttaatac aagatggcgg
     6481 acagttactt cggtcattag gcgttacaat tatgattaca gtgattggaa cgttgattac
     6541 tgtttttatg acgggaacct atgcttatgt tttatcaaga gcctctttcc cttaccggaa
     6601 gttctttact ttttatcttt tttttaccat gttatttgcg ggagggatgg ttccttctta
     6661 cttagtgatg acgaagatgc ttggcttaaa aaatacaatt tgggctttga ttctacctct
     6721 tgcttttagc ccatacaatg tcattatttt aaggacattc tttaagaagt cgattccaga
     6781 atcgattatc gaatccgcta aaatggatgg ctgtagtgag tttcgagttt ttttccaaat
     6841 cgttttgcct ttagcgatcc caggggttgc aacaataggt ttattttcaa gcttaggtta
     6901 ttggaatgac tggttcaacg cattgctgta tattgacacc aacaaattga ttccattgca
     6961 atatttgtta atgaagattc aaaattcaat ggaattctta gcaaataata atgacattac
     7021 attagcgcag caacaagcaa ttcaaaatag ccttcctcaa gaatcaacac gaatggcaat
     7081 ggtcgtagta gctactttac cgattgcaat tgtttatccg ttcttccaaa aatactttgt
     7141 tcaaggattg acgatcggtg gagtgaaaga ataaaaaaga ataggagatg ttaaaagatg
     7201 aaaaagaaca tgcagaaata tttgattggt ggtgcttctg tactaaccct tggcctatta
     7261 ggtgcttgtg gtagcagtaa tggcgaagca aataatgaaa caggaaccac aaaagttact
     7321 tggtatcagg ttggagaaga acccaaagat tttgatgagg tcatgacaaa agtcaacgaa
     7381 aaattaaagg aagaaatcaa tgtagagttg gatatgcggc tgattccgga cggggattat
     7441 cagcaaaagc ttggtgtgat gatcaattca ggagaagaat atgatatttg tttcgtcaat
     7501 ggcacagatt atgtgaatta tgggaacaaa ggtgctttca ttagtctcaa cgatcatttg
     7561 gataaagagt tgaaagatta tgcagctgaa ttgaatgaag gatttattga aggtggggca
     7621 attaatggcg aaacttatgg attgccagtc aataacgata ttgccacaac ggaatattgg
     7681 acctttaata aagatttggt agatcaatac caattggatg tagagaatgt tgaaggtgta
     7741 gaaacattgg ctccagtatt agaaaagttt gcagaaaaaa atactgatac gaatattacg
     7801 ccaatcggta tgacccgtac cagtgtctgt attccttatg acaatattat cggtggtggt
     7861 gtaccgctag cggttgcatt tgaaggcgat acacaaacag ttgttaatac ttatgagact
     7921 ccagaaatga tgacttattt gaaggctatg catgagtttt ataaaaaagg atatattttg
     7981 aaagatgcag ccactgttac gacagatcca tttcgaattg atagtaaaaa ctggttttta
     8041 ggtcgtaact caagtggtta tcaaccatta gcacaaaaaa aattagaagc tctagcggga
     8101 catggattaa ttttaaaacc agctggaaaa gcttttaaaa cgactggatt tgcaagagtg
     8161 gcaatgcatg cggtatctgc ttcaagtaaa catccggaag cagccttgaa attgttgaat
     8221 tatgtgaata ccaatcaaga agtagcggat ttaatggttt atggaattga aggaaaacac
     8281 tatgatcgca atgaagatgg ttcgattact ttaacaaact ctgatgatta ttatcaaggc
     8341 gcttgggcat tggtaaatac ttatctagtt tcaaaaactt caccgattac agaagaaagt
     8401 aaaaaactag aagaagaaag taaaaagttt caaaatgaag caccgatgtc tcctatttta
     8461 ggtttctctt ttgacgcttc tgattttcga acagaaattg caagtgtaca aaatatcgtg
     8521 tcacaatatg aagtactttt aagctcggga acagttgacc ctgaacaata tgtaccagag
     8581 ttgttagaaa aactagaaaa agctggattg gataaaattc aggcatctgc tcaagaacaa
     8641 tacgatgaat ggcgaaaatc taacaaataa ttttttacta aatgaaaaga atagactaag
     8701 cagttagtgg ggggattcaa atgaacgaaa acaaatggac caaaggattt aattgggttt
     8761 ttcaattgtt gttactaaac tttacttatt ttatactaat ttgtttagga tttggaattt
     8821 taggtttttt ccctgctacg atagctttga ttgctggcgt tgtgaatcga aaaaaatatg
     8881 gcttgcaaga gtctgtgaag ttcatgagta aaatctataa gaaaaatttt gttagatata
     8941 atctatattg ctggtcagca actttgttgt tggccggcaa tattactacg atctggcttt
     9001 gttggcagca aagcgcacca ttgttttatt tattaagtac attattatcg gtaattacac
     9061 tgttcctagt ctttgcaatg ttgaattggg tcgtgatcaa aagtcttggg ttgtggatga
     9121 tgggagaccg actgattgac tatttgcttt tcacagcaac gatgttgcca acggtagtca
     9181 tgcaaggctt ggtgcttgga atttatttcc gaatattgat gattttgcct gtgatttttc
     9241 tgtttggcgg aacagctgta acggtaatat tgattagtaa attaactgaa agagcgttag
     9301 caaagttgcc ttgtcagata caatcgcact aattttttca caaaaaggag tatttctttc
     9361 tatgaagtta actgataaac aagtagctaa tatagccttt atccagcaac ttgagattcc
     9421 attgacacaa aaagatgaag aacaaataga cttgattttt actgacacaa aggctaatgt
     9481 gcgacaaatc aagattacta aaaatgagca gcaacgaatt tgcttgtctt attcacatga
     9541 agacttgctg tatcgaggat tgtttatctg ccttccacgt tttttagctc aaggaggaga
     9601 gatgccatac attgaagaaa cctcgatcat tgagaccagt tattcattag atgcttctcg
     9661 taatggcgtg atgaataaag agactctaaa aaaattcatt tcttacttag cgttgatggg
     9721 gtacaacaca ttgtatattt acacagaaga tacctatgaa gtagaagacc ttccatattt
     9781 tggttacctt cgcggagcgt atacaaaaga agaagtgaat gaaattgttg aatttgcttc
     9841 tggttttggc atagaagtag ttccatcgat ccagaccctt gctcacttga cccaattttt
     9901 gaagtggtat gctcaacaag aactgatgga tgatcaaaat actctactga tcggtgaaga
     9961 aaaggtttat gcggcaatcg aaaagatgat tattgcctgc aaagaaatgt atcagacaga
    10021 gcgtattcat ttgggaatgg atgaagccta tcaagcaggt ctgggacgat atttaagcct
    10081 gtatgggtat tgtgatcgga caactttaat gattcaacat atggaccgtg tgttatcaat
    10141 tgttgataaa tataagatga aaccaatgat ttggagtgat ttcatttata agctattaga
    10201 tgaaaccggt aaagataggt attacaatcc tgattttaaa ctcaaagcat tagcagctaa
    10261 aggattacca gcaggattaa cctatgttca ttgggactat ggttcagatg aagtttcctc
    10321 ctatgagaaa gtaattgata accatttgaa attttgtgac attgatcatt acgttttagc
    10381 tagtggtgcc catatctggg ggagactagc cccgaatcat gggaaaacag aagcaattgt
    10441 ttctgcagct gtaaaagctt ctaaaaataa aggtgtacgt tctgtgatgt taactacttg
    10501 gggagatgaa gggcaggaaa cagagcattg gcattcctta attggggcat taaaattagc
    10561 agattatgtc tattctgaag aagagtggaa tgattttgag gaaatctacg atcgtttatt
    10621 aggaacaggt agttatcaat taatgtccga attacgatat ttcgatgaag tggataccat
    10681 tacaaataac aacttacaaa tggcttccgt gagtaaactg ctactttggc aagatccgtt
    10741 agttggtata tatgactttc atgtagccgc tcataacaaa attgccacta gaacaattgg
    10801 tacttattat catgagttag ctcagaaatt agatatgaat aaaaattgga atccaaaaca
    10861 accaatttta acaactattc gtcaacgata tatactttta gctgaaattt tagcacaaaa
    10921 ggcagaacta ggcatctatt tacaacactg ctgtctggta gaagaccttg caggattaat
    10981 ggaattagcg aatcggtgtg aagcactagc caatcgagta aatgaattgg catctattca
    11041 tgaaataatt tggtatcaaa catacaaaac ctatggttgg gaagtattag aacaacgata
    11101 tgctgcatta gctcatcgtc tgaaaaccgt tgcgcgtcgt attaaaaatc atcttcaggg
    11161 gcaagatgcc ctagaggagc ttttggaaga acgattaccg ttttgtcaat atcaagtgcc
    11221 gcttgaaatt tctggcttta attatcggca aagctcggtg tcaggctaca attaacgaag
    11281 gaatcttccc gttgaacgga ccacttgctc atctaatggg aaagtggttc gttttctatt
    11341 cataagtagc ttaccactaa ctcttttatt aagaagagaa aaaaagtagg aagtgagaaa
    11401 atatgttatc ggactattat caaaatctgt cagttataag tgtcaataca gaaccattac
    11461 gtaattatta tattccttat gcaagtaaag aagaagcaaa acaagagtta gaaagggaaa
    11521 aaacgagcca gtttatcgat ctcaatggta catgggattt tcagtattat ccttctgtcc
    11581 aacagttacc ggaaaatttt tttcaagagg cctttactga aagctatgat agaatttctg
    11641 ttcctggagt ttggcaaaca gagggatttg acttacctca gtatatcaat gtatcttatc
    11701 caattccttt tgatccgcct tatgtaccta atgatacccc ttgtggtgta tatcggcgtc
    11761 agtttcaaat tgagcaaaca gatgcacgct tttacttaaa tctagaagga gcagatagta
    11821 attgttttgt ttggataaat agaaactttg taggctatga ccaagtgagt catagtacat
    11881 ctgaatttga tgtgaccaag tatttacagg ttggggttaa tgaaattcaa attctagtga
    11941 tgaagtggtg tgatggtacc tactttgaag atcaggataa gtggcgaatg agcggtctat
    12001 ttcgtccagt gtatcttctt caacgaccaa ctatgcatct gacaagttat caactacgga
    12061 cgttgactga tcctaaaaaa ggaagcggaa cattaatggt ttcttttcaa cgatttactc
    12121 ctattgaagt atcgttacct atttcttacg aattattcca tcctgatgga cgtctcatag
    12181 cgaaagggga gtgttactcg gaacaacaaa taaatttgga tgttgaagat tgcctttttt
    12241 ggacagcaga aacgcctaaa ttatatacat tacttttaac atgtaatggt gaatatttta
    12301 aagaaaaagt tggctttcga gcactgacga tcaagaatcg taagttattg ctgaacggaa
    12361 cacctattaa attatatgga gtcaactatc atgacgtgaa tcagcaaggc acgccagtga
    12421 tgacccgaga gcagttacgt gcagatttgt tattaatgaa aaaattaaat gtgaatactg
    12481 tacgaacggc acattaccct aaatcaccag atttttatga attggcggat gagattggtc
    12541 tgtatgtaat caatgaagca gatttggagt gtcatggtgt ggttaatcta tatgatactg
    12601 aagcgaatta tcaactaatt gctaatgatc cgacattcga aaagccgatt atggaccgag
    12661 tgatccgttc tattcttcct tatcaaaatc gaagctctat cattatgtgg tctatgggga
    12721 atgaatctgg ttatggagta aattttgaaa aagctcaagc tttagcacgg aaattggatg
    12781 attcacggtt gattcataat gaacgctcga ttgaaccagt tattgaccaa gtgaatgatt
    12841 ttagtaactt ggatgttatt agtcaaatgt atcccccact ggaagatgtt catcgtcatt
    12901 gtcaaagaag tgatactgaa aaaccttttt ttatgtgtga atatagccac gctatgggca
    12961 acggtcctgg agatcttaaa acgtacttcg atttgattca acagtatgat tcctttcttg
    13021 gcggttgtgt ctgggagtgg actgatcatg caatcaatag aggaacttta gaaacgccat
    13081 cttatttata cggtggtgat tttggtgaaa aatatcacga cggcaatttt tgcgtagatg
    13141 gtttattgac cccagatcgg caattaacag taggtgcatt ggagtaccgt aatattcatt
    13201 cgcctattca actgattgct gtagatgagg agctgcaaga agtaaccttg agcaatataa
    13261 ctgcttttac agccggttca gcatttaacc tttctcttga atggtatata gatggaaaaa
    13321 aaacagttta tgaagtaatg aatcctttca tacttgagcc tggcaaaagt tgttcattgc
    13381 ccatcacagt acctacaacg attcatggtg ctcatacggt tgctgtttcg ctatctgttt
    13441 ataaaaatga tgagctttat ggacagcaac agctctttct taaaaaagaa aaaatgagat
    13501 tgccaagaac tgctcatcat aatgatgcac cacaaatttc agtaaaaggc acagagctga
    13561 taatcaaagg agaatccttt atttatatat atcaattagc aaaagctgcg ttcacttctg
    13621 taaagatcaa tcaaaaggaa cttttggaac gtcctactca attttcaatt tggcgtgcac
    13681 caacggataa tgatcgtaac ttagtaaaca aatggaaagc agctggatat gctgaaatgt
    13741 ctttagaaat cagaaaaaaa gactggaaat tcgtagatga tcatctcctg atcgaaacgt
    13801 cttatcgagg agttattcca ggattacagc caatttttac aatgcacata cagttgaaaa
    13861 taaatggtgc aggcgtaatc aaccaaaaag tatcagtgga gaaaaatccg atttttccag
    13921 atttccctcg ctttgggatg gactggccgt tgaatgatac tttttcgaag gtttcttatt
    13981 gtggctatgg gccgtatgaa agttatcctg ataagcacta tgctgcgatg tttggtattt
    14041 atgagatgga gattgaggaa tttgatcagc catatatcaa accacaagaa tatggtaacc
    14101 gctggcaatg tgattgggta tcaatcaaaa atcatcaagg atctaccatg acggtttatg
    14161 gaaactctaa tttttctgct ctgccttata gtcaagctca attaaccgaa aagtcacacc
    14221 attatgaatt aataaaagaa aatagaactt ggttcaattt aagttataaa caaaatggta
    14281 tcggctcaca cagttgcggt ccttatttag aagatcgttt ccgttttatg gaagatactt
    14341 ttgactggaa tatagtgatt actttcaaag gagaaaatag atgaaagaaa caaagatcaa
    14401 tcaagtcaaa aaaaaattat ctcgttatca agaagttgtt tatcgaaacc attgcttaaa
    14461 atggtcaaaa aataagctga atgttcgttt taatggatta ttttatcgtt tcgaacagtt
    14521 agaagaagtc tggaaaccgg taacgaattt ccaatcatgt cctgtacagc tcataaatga
    14581 tccaaaagaa acatacgatt tatgtataac acacctttat aaccagcatt tgaaaaatga
    14641 cggattttat ttaaaggtta ctaatgacaa tcaacttacg attgaatcag caaaccttcg
    14701 agggtttcaa tatgctatgg aagccttatt agagagtttt ttttcggtag gagatcaatt
    14761 attattaccg gaattgattc tgaaacatga accatcagtg aaaattcgag gaatcattga
    14821 agggttttat ggtatcccct ggacacatga aatgcgcctc tctctttttt catatatgaa
    14881 agataaccaa ttgaatactt ttatgtatgc ccctaaagat gatgaattat tacgtaaaaa
    14941 atggcgtgaa ctctacgatg cgcaagaatt agacaaattt aaggagcttt tatctgctgc
    15001 tgaggctagc aatatcgatt tttggtactt aattagtcct ggaaatgata ttgatattac
    15061 gtgtgaagaa gacattcaag ttcttctgaa aaagttagaa caactgattg agcttggcgt
    15121 atttcaattt ggtctattaa tggatgatat tgactatcaa ttaaaaggtg ctgctaaacg
    15181 ccgttttcga gagccagcat tcgctcatgc ctatttagtg aatcgagtag aggaatatct
    15241 tagcactcgg ttgtccaatc atgaattagt catttgtcct acagaatatg ataatcgtca
    15301 tggttctcgc tatttagcaa cgttaagtca agaaatacca gaacaattac cgttcttttg
    15361 gactggccca agcacgttag cggcgtcaat ttctacagaa gagctgcagg aaatggcttc
    15421 tgtctatcaa cggccgatgc tgatttggga taatattccg gtaaatgatt atttagagga
    15481 taaagaacta ttgttcatga gtccatatga gaatcgaaca ccgaatcttt ctaaggagag
    15541 ataccaggtt acaggagtag tttcgaatcc aatggctcaa ttagaagctt caaagtttac
    15601 gatcaatagt atggctaatt acttatggaa ctgtgagcgt tttgatcctt tggaaacttg
    15661 gacgtcagtc gttgaaaaag tagttggaag taaactacaa ccagcttatc ttaccttaac
    15721 caatgctttt ccaaatcatt acacaagcag tctgctttca gatgaagaat tactgctagc
    15781 caaagaccca gaatgggtca caaaagaaat ggctgcttta gtccaagcag taaaatggat
    15841 ccagcaagaa caacatacat ctagattgag aacagaattg gagccttggt tacaagcaat
    15901 cacagaaagt tggactttct ggcaagaatg gcaattaaaa aaagatccaa aagaagcaga
    15961 agaatggttg gctaagaaaa aaacacaccg cataggacgt gatttagtaa ctgcccattt
    16021 agagcaaatt atcaaaagtt agatatctta aaaaataaaa agcaagaaaa aggagaacaa
    16081 aatggtcaag agattaatta gtgcaaacta cagtgatgtc aagaaaatga cgtcaaaaga
    16141 gttgaagcaa tccatcaaag cgagtgaagg acgggtgatt gtttgtgaaa cggttgctaa
    16201 tactcttcca caatctggag aaattacgaa tgcagaagtt gcgtcagcat ttggtgcaga
    16261 tttgattctt ttaaatggtt tcgattgtta tcgtcctgca gtttttggac taccaaatgt
    16321 gacgattgag aaggtttttg atctacagca aacgattgaa aacccgattc ctaaattaaa
    16381 agaactagtc ggtcgtcctg tgggaattaa tttagaaccc attgccccta aaacaaatat
    16441 gatgagtgag aaagtagaaa tcagccaagg aagagcaagc tctgttgaaa cgatacaaaa
    16501 agcagaagaa ctaggagtcg attttatttg ctttaccggt aatccaggca ctggagtaac
    16561 caatttagaa attactaaat ccgtaaaaat ggcaaaaaag tatttttcgg gattgattat
    16621 tgctgggaaa atgcatagcg ccggctcaga agagccagtt gtgaataaag aggcgattat
    16681 ttcttatgcg gaagcaggcg cagatatttt actagttcct tcagtagggt ctatcccagg
    16741 ctttttagag caagacctaa ttgaagcggt tcggttggca aaaaaatatg atttgttaac
    16801 gatgtctgca atcggtacta gtcaggaaag tgcccgacca gaaacattgc ggcaattagc
    16861 cattatgaat aaaagttgtg gtgttgattt acaacatatt ggagatgctg gatacggggg
    16921 attggcacca gcagaaaata ttttcgaaac ctcggttgca attagaggaa tgcgacatac
    16981 catcaactgc gttagccgct cgatcaatcg ctaacaaaat taaataaaaa atggttaaat
    17041 tgattattta agtgggaaca tacttgagat atcagctaac ttctgtcact ttaaaaaagg
    17101 gactgtgaca gaagtgaaaa tagacaaaaa atatccgaac aagagactag gatagcagaa
    17161 aaactatcgt tttgcaacga ggaccaactg gaccgtagga gcaattctct tattgttcgg
    17221 atatttcgct tgttcaagga cttatgtctg tagtgccctt ttttggtagg aaaaatggta
    17281 gggaaccata tcatttttaa tgaaaatcaa gcatttcaaa agttgaaa
//
LOCUS       DACDNO010000058        17005 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDNO010000058 DACDNO010000000
VERSION     DACDNO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.058"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..124)
                     /locus_tag="IWI22_002112"
     CDS             complement(<1..124)
                     /locus_tag="IWI22_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9169489.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..539)
                     /locus_tag="IWI22_002113"
     CDS             complement(228..539)
                     /locus_tag="IWI22_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9169490.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(541..738)
                     /locus_tag="IWI22_002114"
     CDS             complement(541..738)
                     /locus_tag="IWI22_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9169491.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1132..2859)
                     /locus_tag="IWI22_002115"
     CDS             complement(1132..2859)
                     /locus_tag="IWI22_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9169492.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2852..4045)
                     /locus_tag="IWI22_002116"
     CDS             complement(2852..4045)
                     /locus_tag="IWI22_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9169493.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4232..4876)
                     /locus_tag="IWI22_002117"
     CDS             complement(4232..4876)
                     /locus_tag="IWI22_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9169494.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4970..5104)
                     /locus_tag="IWI22_002118"
     CDS             complement(4970..5104)
                     /locus_tag="IWI22_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9169495.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IWI22_002119"
     CDS             complement(5106..7238)
                     /gene="feoB"
                     /locus_tag="IWI22_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9169496.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7235..7708)
                     /locus_tag="IWI22_002120"
     CDS             complement(7235..7708)
                     /locus_tag="IWI22_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9169497.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8109..8333
                     /gene="nrdH"
                     /locus_tag="IWI22_002121"
     CDS             8109..8333
                     /gene="nrdH"
                     /locus_tag="IWI22_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9169498.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8492..10651
                     /gene="nrdE"
                     /locus_tag="IWI22_002122"
     CDS             8492..10651
                     /gene="nrdE"
                     /locus_tag="IWI22_002122"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9169499.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10701..11666
                     /gene="nrdF"
                     /locus_tag="IWI22_002123"
     CDS             10701..11666
                     /gene="nrdF"
                     /locus_tag="IWI22_002123"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9169500.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11755..12894)
                     /locus_tag="IWI22_002124"
     CDS             complement(11755..12894)
                     /locus_tag="IWI22_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9169501.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13203..13916)
                     /locus_tag="IWI22_002125"
     CDS             complement(13203..13916)
                     /locus_tag="IWI22_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9169502.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IWI22_002126"
     CDS             complement(14170..14871)
                     /gene="nagB"
                     /locus_tag="IWI22_002126"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9169503.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15105..16637
                     /locus_tag="IWI22_002127"
     CDS             15105..16637
                     /locus_tag="IWI22_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9169504.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16828..>17005)
                     /locus_tag="IWI22_002128"
     CDS             complement(16828..>17005)
                     /locus_tag="IWI22_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9169505.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3494 c         2909 g         5324 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt
      541 ttagcacttc cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca
      601 agtagttgcc ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc
      661 ataatatttt ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa
      721 aatttttaaa tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct
      781 aacattcatt aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa
      841 taagaattta atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa
      901 tgcacttcgt acataataaa ctttaatcac attagtcttt catacctacg atcgatctct
      961 ttgactcgca cccataaatt cataatagtt tgtcggcgtc tttgaacgtt tcgaagatga
     1021 gctacgcatt ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat
     1081 ttggatattt gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt
     1141 ccagctttcg actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc
     1201 ttttccattg ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt
     1261 gtctaatact ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg
     1321 gatctgctct tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt
     1381 ggcaaacatc caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc
     1441 ttgacctagt gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa
     1501 gttgtcatat ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat
     1561 tcctatgatt ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta
     1621 ggctggcact gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg
     1681 atctttatat tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc
     1741 atctgactcg ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt
     1801 tacatcatag cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt
     1861 atcccattca acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc
     1921 tataccaatc gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt
     1981 tttacttttc tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg
     2041 tgtaaaacgg tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt
     2101 cccgacttgt gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat
     2161 catagaggca ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat
     2221 gatcgtactt ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc
     2281 gaatgaatca gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa
     2341 acgaacggtt ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat
     2401 accgaaaaat gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc
     2461 aaggatcggt ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg
     2521 taccccaatg atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa
     2581 atcattccat agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag
     2641 tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg
     2701 agctggaatc actaacagta aatttgtaat tcccgacatc actgcctgtc caatcgtttg
     2761 taaaaatgaa aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca
     2821 gtttgccact ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt
     2881 ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta
     2941 caactgcaag tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt
     3001 ctttcccaat caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc
     3061 tagcttctaa cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag
     3121 ccatcagcat gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag
     3181 attcgatatt cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac
     3241 gatcgacttc ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt
     3301 cacctgttcc gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc
     3361 gtaaagcttc attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca
     3421 ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt
     3481 cgtccatcag caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt
     3541 gcatcccacc agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt
     3601 ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc
     3661 cgtattctgt attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca
     3721 tattgatttt atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt
     3781 ctccatcaat atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca
     3841 atgtggattt cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt
     3901 caaaattcac gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg
     3961 gctggtgatc attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta
     4021 aatgttttac ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct
     4081 ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag
     4141 aaatttgctt gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc
     4201 caagttaata tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa
     4261 aaaagtttgt catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg
     4321 ccgtaaattt agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga
     4381 taactgtggc gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca
     4441 ttgctttttc tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct
     4501 tcgtttgatt gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct
     4561 tctccacact tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt
     4621 gcaataggaa atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta
     4681 cttccatgat tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag
     4741 cattgtacgt actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt
     4801 tttcaacgat acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt
     4861 ttttctttcc agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc
     4921 tctagcaaaa taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga
     4981 tctgttcctt ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta
     5041 aacgagtata gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg
     5101 ccatcctatt cctctccttc taatgacaat atagtataaa actggttctt ggattttggc
     5161 tgacggaaca aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac
     5221 gctacctgtc caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa
     5281 gctaaaccgc attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga
     5341 tggatagccc caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa
     5401 taagctgctg cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg
     5461 gatacttccg catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc
     5521 aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca
     5581 actgccagga ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca
     5641 taaaaattat aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct
     5701 gcttttttaa caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga
     5761 ggaaaatgat atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt
     5821 gtttttttca agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa
     5881 ggagctaccc aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac
     5941 tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt
     6001 tcaatcgtgc gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga
     6061 atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc
     6121 ctcgccatat atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc
     6181 ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc
     6241 atccatcccg ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta
     6301 ccaaacaatg tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga
     6361 taatagatca gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca
     6421 atctgatcga ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt
     6481 gtacatagtt gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg
     6541 tccaatagtt tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct
     6601 atctcgtaat cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt
     6661 ttcttgccgc tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt
     6721 ggatcatatg tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt
     6781 tcgattagtt tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca
     6841 agaccatagg aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc
     6901 atattcagac caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga
     6961 tttcgttcta aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc
     7021 aaataatctc ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt
     7081 aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt
     7141 acgcccggcc aattccctac atattgattc gaaccagtca agagattgaa caagctagtt
     7201 ttcccgctgt ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca
     7261 agattagttc ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga
     7321 ttggatctcc cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca
     7381 tatccattaa ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct
     7441 ctcctgcttt taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga
     7501 caaagatttg acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa
     7561 tggcattgtt gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat
     7621 tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat
     7681 tctgctttgc ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta
     7741 ttaaaatcat tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca
     7801 ttctcacata gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc
     7861 gaatctttag tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa
     7921 ttttttctct attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca
     7981 aaaacacaaa acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg
     8041 acttttttac taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg
     8101 cactcaccat gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca
     8161 aacggttttt aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg
     8221 aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg
     8281 cagaaacagt tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact
     8341 aaaagaatca aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg
     8401 caaacttcca aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc
     8461 atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag
     8521 ttatttcaag ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa
     8581 ggataaagaa gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt
     8641 tccatctatt atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt
     8701 cattcaattg tattcaccat catttattga aaagctatat caatttttgg acgagcaaca
     8761 tttttctttc aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac
     8821 caacgacggt tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta
     8881 ctttgcgaac ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg
     8941 atatcagcca gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt
     9001 gtcttgtttc ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc
     9061 tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga
     9121 agccggcgct cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa
     9181 attattcgaa gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt
     9241 ggtttatttg aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa
     9301 tgcagatgaa aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt
     9361 ttatgaattg acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa
     9421 agaatatggt gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc
     9481 aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa
     9541 attacaacaa gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa
     9601 tccaatcgat ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac
     9661 gccttctgtg attaacggca aacaggaata tgaagtatta ggaacagata tctcatgtaa
     9721 cttaggctcg acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg
     9781 tacgatgact cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc
     9841 gatccaaaac ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac
     9901 ttatcttgca aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat
     9961 ttacttcatg ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag
    10021 aaaacaagtt ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa
    10081 atacgtaact ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat
    10141 ttttatccca actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact
    10201 gtatcatcaa aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac
    10261 gagtgcaagt attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg
    10321 taaaatttat tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc
    10381 ttatgatatg gacatgcgta aagtaatcga tgtttatgca acggcacaac aacacgtcga
    10441 tcaaggcatg agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg
    10501 gaagacaaca acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca
    10561 caaaggaatc aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg
    10621 aagcaatcaa tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt
    10681 ttaattaaag gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa
    10741 ttgaagatat cattgataaa tccacttggg agaagctgac agagcaattt tggctagata
    10801 cacgtatccc actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag
    10861 atacagtagg acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg
    10921 gcatggatca gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata
    10981 ttcaattcat ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata
    11041 cgaaaaaaga aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa
    11101 aagctgagcg tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca
    11161 gcgtcttttt agaaacattc ttgttctatt caggttttta tactcctctt tattatttag
    11221 gaaacaacaa attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg
    11281 tacatggaac ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag
    11341 aacaagaaac gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag
    11401 aacgttatac agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc
    11461 tacgctataa tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata
    11521 ctgccaatga tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg
    11581 acttcttctc acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg
    11641 acgattattt gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat
    11701 aaaaagagtt tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc
    11761 acttgtttct tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct
    11821 caataaatag attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat
    11881 gcaaatcagc atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat
    11941 cgtcaaagga tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc
    12001 taattgttgg atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa
    12061 caaaccgaca atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt
    12121 cgtgattccg caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc
    12181 caaaaagctc attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga
    12241 taatgtttcg tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa
    12301 gaagtgagac aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa
    12361 tggaacggta aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact
    12421 tgcagataaa ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc
    12481 taattgttct tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca
    12541 tttgcttgtt tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat
    12601 taactgaggc actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt
    12661 caataaaaaa ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc
    12721 gaacatataa tagataaaac cagcaacaag caatggtaaa aaaattgccg aaagcatttt
    12781 caatattgga ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag
    12841 tactagctcc actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct
    12901 cctccaaggt cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt
    12961 ttcatttgct taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta
    13021 aacaaaaaca cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa
    13081 acggtgttca aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac
    13141 tatttttatt tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc
    13201 atttattgcg caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc
    13261 tcgatcacat cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac
    13321 actcggtagt catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc
    13381 tcttcaaaat aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct
    13441 aactttttag tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc
    13501 ccgataaaac tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca
    13561 gcaattgccc ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg
    13621 atcgttcccg cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca
    13681 tctgaacgaa tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta
    13741 gcgatctcaa cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta
    13801 cgcatacctg ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc
    13861 atagcgatcc ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa
    13921 acaccctcca gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta
    13981 cctcttacga aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg
    14041 gtgttcaatc agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt
    14101 ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac
    14161 tattgaaaat tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg
    14221 attttgcaat acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat
    14281 tgcatcagct ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga
    14341 accgattccc attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt
    14401 gtttgcattg attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc
    14461 accaggttca ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat
    14521 tggatgtgca tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt
    14581 tccatctggt acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa
    14641 atgacggtag ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga
    14701 agtcatttca ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt
    14761 gcttcctgtt gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc
    14821 aaatgctttt tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc
    14881 tcctttatat tttggtatag cccaattata gcattctttt tttcttttta caataattag
    14941 tatagaccaa attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt
    15001 tcataagatg gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg
    15061 tggacgcttt atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat
    15121 taaacaacat tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat
    15181 taaatgctgg cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga
    15241 ttaaaatcat cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata
    15301 ttcacaaagg ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat
    15361 ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc
    15421 aaacatatag cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg
    15481 aattagacca aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt
    15541 tggggatcga tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt
    15601 caaaggtgat tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac
    15661 caaccaatca cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg
    15721 acggtgcgta tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata
    15781 tcgcagacat cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga
    15841 aacaaaagga acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa
    15901 tcgaaaagac cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa
    15961 aaagccgaca aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta
    16021 aagctgtctt tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg
    16081 atttagtcat cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg
    16141 gtgaaaatat tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac
    16201 tgatcggtga gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca
    16261 attacttttc acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa
    16321 gcagtcaatt tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt
    16381 tagttaatca attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg
    16441 tgaaacttgc acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga
    16501 accatatcga tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa
    16561 cagtaatcat cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg
    16621 agattgaacc aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt
    16681 tttgtcacaa ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc
    16741 tcaaaaaacg gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca
    16801 gaacagcttt tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt
    16861 aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac
    16921 acctaaccag ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc
    16981 aataaagtac ataaacataa ataca
//
LOCUS       DACDNO010000059        16837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDNO010000059 DACDNO010000000
VERSION     DACDNO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.059"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..573
                     /locus_tag="IWI22_002129"
     CDS             <1..573
                     /locus_tag="IWI22_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9169506.1"
                     /translation="KNGIDADFVSPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRAL
                     GNTLRPDDVDANDYAAVYYAGGHGTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVG
                     LLPVTDESGKSILAGRTVTGFSNEEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSE
                     VVQIDGRLLTGQNPQSAHQLGEAVVEALKN"
     gene            905..2200
                     /locus_tag="IWI22_002130"
     CDS             905..2200
                     /locus_tag="IWI22_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9169507.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            2190..3650
                     /locus_tag="IWI22_002131"
     CDS             2190..3650
                     /locus_tag="IWI22_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9169508.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            3786..5282
                     /gene="glpK"
                     /locus_tag="IWI22_002132"
     CDS             3786..5282
                     /gene="glpK"
                     /locus_tag="IWI22_002132"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9169509.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            5296..7119
                     /gene="glpO"
                     /locus_tag="IWI22_002133"
     CDS             5296..7119
                     /gene="glpO"
                     /locus_tag="IWI22_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9169510.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            7120..7836
                     /locus_tag="IWI22_002134"
     CDS             7120..7836
                     /locus_tag="IWI22_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9169511.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            8142..10070
                     /locus_tag="IWI22_002135"
     CDS             8142..10070
                     /locus_tag="IWI22_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9169512.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            10196..10750
                     /locus_tag="IWI22_002136"
     CDS             10196..10750
                     /locus_tag="IWI22_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9169513.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11235..11549)
                     /locus_tag="IWI22_002137"
     CDS             complement(11235..11549)
                     /locus_tag="IWI22_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9169514.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11563..11823)
                     /locus_tag="IWI22_002138"
     CDS             complement(11563..11823)
                     /locus_tag="IWI22_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9169515.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            11993..12307
                     /locus_tag="IWI22_002139"
     CDS             11993..12307
                     /locus_tag="IWI22_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9169516.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            12309..13964
                     /locus_tag="IWI22_002140"
     CDS             12309..13964
                     /locus_tag="IWI22_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9169517.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            14047..16182
                     /locus_tag="IWI22_002141"
     CDS             14047..16182
                     /locus_tag="IWI22_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9169518.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            16357..16722
                     /locus_tag="IWI22_002142"
     CDS             16357..16722
                     /locus_tag="IWI22_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169519.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYAVCIIKRDK"
BASE COUNT         5648 a         2853 c         3680 g         4656 t
ORIGIN      
        1 aaaaatggga tcgatgcaga ttttgtcagt cctaaaggcg gatatgtacc tttagaaccg
       61 caaagtgttt tgaatatgga tgaaatcgat tgggcctatt atgaagatga agatttcaga
      121 aatcgggcct tgggaaatac cttacgtcct gatgatgtgg atgcaaatga ttatgcagcg
      181 gtttattatg ctggtggcca cggaacaatg tgggattttc cagaagcaat agatcttggg
      241 aaagtcgctt cacaaatcta tcgaaatgaa gggctggtct cagctgtttg tcacggtgtt
      301 gtaggattgt tgccagttac agatgagtct ggaaaatcta ttttagctgg acgtacagtg
      361 actgggttca gtaatgaaga agaggaagca aatggtacaa cgaatgaagt accattctta
      421 gcagaagatg ctttgaaaga aaaaggagca aactatcagt ctggagcagc tttttcagaa
      481 gtggttcaaa ttgatgggcg gcttcttaca gggcaaaatc ctcaatctgc tcatcaatta
      541 ggcgaagcag ttgttgaagc attaaagaat taaaaataaa tgatgtgtct attctaacta
      601 ttacattaca cacgctaaaa ctcgtctttt cgttcttttc tgtttcctat ttggacagag
      661 gtacgtaaaa ggcgagtttt tttcttttat aagagtacat tttttgaaac atctcttcag
      721 aaatgatact cttcaattag gtccactagt acattattga gaagcagacc tagaacacgg
      781 tatgatggag agggttgatt ctgctgcatc gctatgtatc ataaggttga aagcgagtta
      841 tgaatactgt gttttctgat tgagaagtta gcagtagaac accttataag aaagaggaat
      901 gcagatgaac attgtaattg tcggcgggtc gtttgcaggg attcatgcgg ctatttatct
      961 tcgccaattg atgccaacaa gtgaaatata tttgattgaa aagcaatcga agttaggatg
     1021 gattcccagc gggttcaatt tgatattaaa aggaaaggtt accagtgaaa aacaactttc
     1081 ttggatcacg aaagaagaac tgactgaccg ctatcgaatc catgtttaca cagaaaagac
     1141 agtaatagga ttgaaagaaa aaaaagtcgt tttagcagga ggaaaaaaat tggattttga
     1201 tcgtttgatt ctagcgactg gttcaaatca aaagtttcga aatatttctg cagaatcaac
     1261 gcttattcac ccagttaaaa agatacaaaa cgtaacggct cttcagcaaa agatcatgaa
     1321 tgcaaagaaa attgcaatta ttggtgcagg acaagcagga atcgaaatag ctgaagggtt
     1381 ggcatcaaga aaaaaacaaa tccatcttta cgaaagccga aagtcaattc tttttcgtta
     1441 tctagatcct gaaatgacag aaccgttagt aaaggaaatg aaaaatcaag ggctttcact
     1501 ttttttagaa gaacaagttg tgtctttgac cgaaaatgaa accgctattg tagagacgga
     1561 aaaaagaaag gagaactatg atcttgtttt gctggctaat cattctcgcc ccgacaatca
     1621 gatgtgggaa gaacaattaa cattgaatga tgatggaacg atctgggtag atgactactt
     1681 gcaaacgtct caaaaagacg tttatgcgat tggcgatgcg atccaagtaa cttttcgtcc
     1741 aacaaatgaa aaaatgtatg tttctctagt aaataacgct gtccgtacag cacgaaatgt
     1801 cagcaagaca attagtggct cacagacaaa ggatcaggga acctatcgtc cagtcggcaa
     1861 tcagtggttt ggctatttct taggaagcgt aggtttgaca gaagaagaga gcatttttta
     1921 tccccaaaaa attgaaacag gatacgtaga ctacaagctt acagcaataa atcaaagcaa
     1981 agttaaactc aaatttctat ttgattctga aaaaaagatt cttggagcac agctccaaag
     2041 caaaaaagag atgttccacc ttcttgacca actgacaatt gcagtaaaag aaggatggac
     2101 gtttgaacag ttagaagaac atgagctatt tttccagcca gaataccgtg tgccagatat
     2161 ggtgtggtca aaggtggcga atactgccta tgaagattga agcattatta gaaagaaaag
     2221 agcagttaca gatcctgatt ttgcgaaacc ttgttctgca agggggaaca gcctctatca
     2281 atggtttgag agagcatgtc caactgtcaa aagcctcgtt tgatcaatat ttggaagata
     2341 ttgaattgat tggccgaatg atggaaaaaa aagtagaagt acagcgaaat gagtatcagg
     2401 cgctgcttgt gttggacgaa gatgtaagtt tggaaaaaat tctattgttt ttattgcagg
     2461 aaagcttgaa gtttaagatg ctcgtctatt tattagaaca tcagcaagta tccatcgttc
     2521 gattagcgac agcgtttaat atcagtgagt cttctgtttt tcgaaagatc aaagaattga
     2581 accagctctt agaggaattt ggattacaaa tcaaaaacgg tcaattatac ggagaagagc
     2641 tacagatacg atatttttat tatgaactat tccaatatat cccggaagat cagcgaccgc
     2701 tttttttgca aaatacacca gaaaaaagac cgttcattct aggattggac cgtgtattag
     2761 aaacaacgtt tacggcatca gcagaagcac agattgcttg ctggctcggg attacgaaaa
     2821 aaagactatt gaatgaaaaa agtacgtacg caactttgaa agaaaagaaa ttactctatc
     2881 agtcggaccg gctttatcaa gcaatcgatc caatcatcgt gatgcatctt agtcgaacag
     2941 cagcagaact gaatgtatac gaaacaatga tgttttacag cttttttgtt tctttttcga
     3001 tagtagacga agaaatattt tatcagtatg atttgacacg gagcaaaaag ttaccaaccg
     3061 ctgttttaga tacttacatt cgggaaacga tgttatggca ttatcgtcct cgtcgtttga
     3121 aaatcaaaga agaaaaggca gtaggctacc aaatttccca gatcaacaat gaatggtttt
     3181 tctttgttgg aaagatcgaa gtctatgagc gtgatcggct tttggaacag cagcagaaaa
     3241 tgctcgggca ttccttaaca cagttactgg cgaaattaca ggaaacagcg gtgcagcaat
     3301 tgccgaggaa aagagctgaa gatagtgagc ttagttacct gatgatccag tatgccaatg
     3361 ttcttttgat gatcgatttt tatattgcaa aaccagtagt gattggaatc gatttagaaa
     3421 gtctgccgat ttatcgtatt gcttttcaac aatatctgat cagagaactg aggggaatcg
     3481 gcggtattga gattggaagt tatgaagaag gtaaagaata tgatttggtg atcacatttt
     3541 gccagagaaa caaaaaacag tgtgaatatt atctgtcaga atttgcgtca ccttacgaca
     3601 ttattcgatt gaaaagaaga atagagacgc tgaaaaaaga aaagaattga ttaaaaaata
     3661 gcttcataat aaaaaactgt caaaaaaaga aaaaaagaat aaaattttga cagtttcttt
     3721 ttttaaaaca gtagtatgat aagagtagtt aaagaaagcg tttgcatata atggagggaa
     3781 tcacaatgac agaatcaacg tatatcatgg cgattgacca aggtacaact agctcacgag
     3841 caattatctt tgacaagaaa ggaagacaca tcggcagttc tcaaaaagag ttcacgcaat
     3901 atttcccgca agaaagttgg gtggaacatg atgcaaatga aatctggaat tctgtacagt
     3961 cagttattgc cggagcgttc atcgaatcag ggatcaaacc aaatcagatt gctgggattg
     4021 gcataacgaa ccagcgtgaa acaacagtaa tatgggaaaa agatacagga cgtccaatct
     4081 atcatgcagt tgtttggcaa tccagacagt cagctggaat tgcagataac ttaaaaaatg
     4141 aaggctatca agaatttttc cacgaaaaaa cagggttagt gatcgatgcc tatttctcag
     4201 caacgaagat acgctggatt cttgatcatg tagaaggcgc gcaagaacga gctgaaaaag
     4261 gcgaattgat gtttggaacg atcgattctt ggttagtatg gaaactgacc gatggacaag
     4321 cacatgttac tgattactcg aatgccagtc ggacgatgtt gttcaatatc catcagctag
     4381 attgggatca ggagatttta gatttactaa atattcctcg agtgatgtta ccaaaagtca
     4441 cttctaactc agaagtttat ggttatacgc aaggttatca tttttatgga agcgaagcac
     4501 caatttcagg gatggcagga gaccagcaag ccgcattatt cggacaaatg gcattcgaac
     4561 caggcatggt caagaatact tatggtacag gttcatttat tgtcatgaat accggtgaaa
     4621 aaccgcaatt atccaaaaat aatttattga cgacaattgg ttacggcata aatggaaagg
     4681 tttactatgc tttagaagga agtatttttg tggctggttc ttcgattcaa tggctgagag
     4741 atggacttca aatgttgcaa aaagctagcg attcagaaga cgccgcgaaa cgctctacaa
     4801 gcgaagatga ggtttatgtt gttcctgcat tcgttgggct aggtgctcct tattgggatc
     4861 aggcagcacg aggggctatg tttggcttga cacgtggtac gacaaaagaa gatatcatta
     4921 aagctacttt acagtcgatt gcttatcaag tgagagatat catcgatacg atgcaagatg
     4981 acactggaat caagattcct gtattgaaag tagatggcgg tgcagcaaac aatgaatatc
     5041 tgatgcaatt ccaaacagat atcttgaatg taccgatcca acgagcagaa aacttggaaa
     5101 caacggcgct aggggcagca tttttagcag gattagctgt cggttattgg aaagatacag
     5161 acgagatccg cgaattttat gaagctggca agctatttga agttcaaatg gaggaagagc
     5221 gccgagaaaa actttacaac ggttggaaaa aagcagtaaa agctacccaa gcatttgaat
     5281 aaggaggaaa tccatatgtt ttcaaacaag acaagacaag atagcattca aaaaatgcag
     5341 caagaagaat tggatctgtt gatcatcggt ggcggaatca ctggtgccgg tgtggcagtc
     5401 cagtcagcag catcaggaat caaaacagga ttgatcgaaa tgcaggattt tgcagaaggg
     5461 acttcctctc gctcgaccaa acttgttcat ggcggtattc gttatctgaa gacatttgat
     5521 gtggaagtag tagctgacac agttggtgaa cgtgcagtcg tacaaagtat tgccccacac
     5581 atcccaaaac cagatccaat gcttttgcca atctatgagg gcgaaggagc aacaaccttc
     5641 aatatgttct cggtcaaagt agcaatggac ctttatgaca aactggcaaa tgtgacagga
     5701 accaaatatg agaattatac gctgacacca gaagaagtat tggaaagaga accgtttttg
     5761 aaaaaagaag ggctaaaagg cgcgggtgtc tatctagatt tccgcaacaa tgatgcccgt
     5821 ttagtgattg ataatatcaa aaaggctgcc gaagatgggg cttatctagt aagtaaaatg
     5881 aaagcggttg gctttttata tgatggcgat caaatcgttg gcgtcaaagc tcgtgacctg
     5941 ctgacagatg aagtgatcga aatcaaagca aaattagtga tcaatacgag tggtccttgg
     6001 gtagataaag tacgaaactt gaattttacg cgtccagtct ctcctaaaat gcgtccaact
     6061 aaagggatcc atttagttgt agatgcgaaa aaactgcctg taccgcaacc cacatacttc
     6121 gatacaggaa agcaagatgg gcggatggtt tttgctattc caagagaaaa caagacttac
     6181 tttggtacga cagatacgga ttaccaagga gattttacgg atccgaaagt cacacaagaa
     6241 gatgtagatt atctattgga tgtgatcaat catcgctatc cagaagcaaa tatcacattg
     6301 aatgatatcg aagcaagctg ggcaggactt cgtccactgt tgattggtaa ttctggttct
     6361 gattacaatg gtggagataa tggatcgatt tcagacaaga gcttcaataa agttgtcgat
     6421 acagtaagtg aatataagga aaacaaagtt tctcgtgctg aagtagaaga cgtgttgaac
     6481 catttggaaa acagccgtga tgaaaaagca ccttctacga tttccagagg tagttcttta
     6541 gaaagagaac cagatggttt gctgacttta tctggtggga aaatcaccga ttaccgtaag
     6601 atggcagaag gagctttgcg attgatacgc caattattaa aagaagaata cggaatagag
     6661 acgaaagaaa tcgactctaa aaaatatcag atttcaggtg gaaacttcga tccaactaaa
     6721 ttagaagaaa cagtgacaga attagcaaaa gaaggagtag cagccggttt agaggaagaa
     6781 gatgctactt atatcgctga tttttacggg actaatgccc gacgtatctt tgaattagca
     6841 aaagaaatgg caccttatcc tggtttgagt ctcgctgagt cagctcggtt acgttatggt
     6901 ttagaagaag aaatggtttt ggccccaggc gattatctca ttcgtcgtac aaatcatctg
     6961 ttgtttgaac gagatcagct tgatgagatc aagcaacctg tgatcgatgc aattgctgga
     7021 tattttgaat ggacagaaga ggagaaggcg caacagacta aacgtttaga agcattgatt
     7081 gcagaatcag atctgcggga actaaagggg gagaaataaa tgaacagtga tatgactcaa
     7141 atcatggggg agtttattgg gacactgatc ctagttttgc ttggagatgg tgtgtgtgcg
     7201 gctgtgaatt taaacaaaag taaagcacag gcttctggct ggattgtcat cgcatttggt
     7261 tggggactcg ctgtaacaat ggctgtctat atttctggat tcatgggccc agctcatttg
     7321 aatcctgcag tctctctagc tatggcgatg acaggggcaa ttagttggaa tcttgtcgtt
     7381 cctttcatca ttgcgcaagt tttaggagct tttgcagggg ccattcttgt ttggctttct
     7441 tatttgccac attggaatgc aacgaaagat gaaagcgcga ttttaggaac gtttgcaaca
     7501 ggtcctgcta tacggaatta tccagcaaat gtgatcacag aattaattgg tacatttgta
     7561 ttagttcttg ggttgctagc ttttggacaa aacgaatttg cacctggaac gaacgtattc
     7621 gctgtaggtg gattgatctt agccatcggt ctttcattag gagggccaac aggatatgca
     7681 atcaaccctg ctcgggactt tggtcctcga ttagcacatg cggtattgcc aatagctaat
     7741 aaaggaactt cagattgggc atattcatgg gtaccaatcg caggtccaat gatcggagca
     7801 attatcgcgg taggtgtcta ttcattaatg gtgtaaatct ataaaacata ctaaagtttg
     7861 acaaccaaaa tgtgtcttgc tatagttaag tggaaaataa ataagaccta gaagaatggg
     7921 gtatgttttg tgagtgtagc tggtatctaa tcagtaagtc gaatagccaa taaacaaagt
     7981 ttatttggtg tgcttatgaa acggtacctg tcaggaaaac aatatctttg tattctaata
     8041 aggataggac atgacttttt taagctgtgt tctatccttt tatttatctc taggccctta
     8101 tttattttgg gacaggtatc tcttcaagaa aaggagagaa tatggacaac gaaatgatta
     8161 gtcaattaca atttgatcgg atcaaaaagg aaatccaagc aagagcaatt ggaaactata
     8221 gcaaaaaacg aatcagcgat atgacggtct cgacaaacct acaaactgtc ttggatcgtc
     8281 aagaagaaac aagagaagca cgcttgatct tagaaagcag tcagcatgtt ccatttatgg
     8341 ggttgccgag gatcgatgca ctgacagaac aagtcaaaaa agggttgatt ctgcagccaa
     8401 ccgatttgat cgaatatgcg gattttctta gaagcagtcg gatgatcaca aagttttttg
     8461 ataagaatca ataccagaca cccttgttgt ttgcttatag caaacatctt cctgatttga
     8521 taaatgtaga agagttgatt gaccagaaaa taaaaaataa taaagtcagt gacgacgcgt
     8581 cacgtaattt gcgtaaagta cgcaaacaat tacaactaat cgaaaaagaa atccagtcaa
     8641 aattactgaa attcttgcgt catccgaaaa acaaagaaat gatccaagaa gcaatgattg
     8701 ttcaaaaagg cgaatactat acgattccta tcaaagccag ctataaaaat aaaatagatg
     8761 gaacgattat cgatgaatca aataaaagaa cgacggtttt tattgaacca actgtgattt
     8821 ctaaattgaa tgaacactat cagttgttaa aagctgaaga aatcagtgaa gaatatcaag
     8881 tcctggcggc attgactggt gctatcgcag aaaacgaaga agtgatcgat ttattgatcg
     8941 aaacgatcac ggtgttagat atcatctttg ctcgggcaaa atttagccga gaaatcaatg
     9001 gaagcacacc tagaatcaat aaatcggaac atatcattat caagcaaggc agacatccat
     9061 ttttacctga gcatgcggta ccattggatg ttgaaatcgg taaagactat cgaggcttga
     9121 ttatcaccgg agcaaatgcc ggaggcaaaa cagtcgtgtt gaagacagtc ggtttattga
     9181 ctttgatggc gatgttcggt atgcaagttc cggcaaaaga agggacagag cttgctgtct
     9241 ttgatgagat tttcgtcgat gtcggtgacc accaaaattt agaaaatgcg ttgagtacat
     9301 tttctggtca tatgcaaaac atcgctgcta ttttgaaaaa gataaaaagg aatacgttgg
     9361 tacttttaga tgagattggc agtggaacag agccaaatga aggggctgca ctagcgatcg
     9421 caatcatgga atctatgtat gagcaagggg cattgatcat tgccacgacc cattatggag
     9481 aaatcaagaa attcgccgga gatcatgaag attttgtgcc ggctgctatg gctttcgatc
     9541 gggaagcact tcgtcctaaa tatcagttgc gggttggaga aacaggagaa agccaagctt
     9601 tgtggattgc tcataaaatg gatatgtcga tgaaattgat ccatcaagca gagcgttact
     9661 tggcggaaaa agcctatcgg acacagaaaa aagaatttct tcctaagaca gcagttacaa
     9721 ataggaagga gaataaaaaa gaacgccagc tctttgcaaa aggtgatcgg atatttgtca
     9781 atgaatacca aaaagaagcg ttagtttatg aagatatagg tgaagatacg attgatgtat
     9841 atttagacaa aaaaataatc cacgtgcctc gtcagcgtgt tcgtctggtg cgttcagcag
     9901 aagatctgta cccgacaggt tacgatttag acagtctgtt tatcgattat aaaacacgta
     9961 aacggcagcg tgacctagag cgaggatcaa aaaaagctca taaagtacta gtaaaagaaa
    10021 tgcgcaaacg acaggaagaa agaagagtaa acgatgaaaa cagcaagtga ttatctcaaa
    10081 ttagctcata atatcagtgg ctgtaaagaa aaataaaaac agtgaaatca ttggttttat
    10141 tccccgtgta agctaaaata aaaagaaaag agtgctgagg aggtagggcg ataaaatgaa
    10201 attaagcaca gactggcgta aagaaataca aacgattccg aatcttttat cgatattccg
    10261 tatttttttg ctgccgattt atctttattt tgtgctgaga cagtcatttt atatagcagg
    10321 agcggtcatt gttgtctcag gtttgtctga ttatctagac ggggtcattg ctcggagata
    10381 taatcaggta acagacttag ggaaggtgct tgatccgttt gcagataaat tgacacaact
    10441 ttttctgatt ttgtctatgg catggtatcg accgtggctt tggctgttgt ttggtctatt
    10501 cttgataaaa gaaggcttta tgtttgtagc tggattaatc gggctatcta aaaatatcaa
    10561 attaagtgga gcgaagtggt atggcaaagt agcgactgct gtcatctatg ttggcatgat
    10621 ccttttgctg cttttcccag aattaccaac gctgtgggtg agagtcattt ttgccgtcat
    10681 cacttacggt cttttacaat cttttgtctt gtatgctgtc gagtaccgaa agatgtttca
    10741 aagaaaataa aaaaggggtg tgacctatgt cgcattcctt tttttgataa atagactccc
    10801 agtagctact tctcgataat aagccaacca aaccaaaaac aggagaagtt gtttttgaaa
    10861 ttatttcttg tgattcggag ttaaatggta ggctcacaac ctcttggtaa acggtgttca
    10921 atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggagct attttcccgt
    10981 ttttttctct aatacttagg tcaaaccgct gatttcacaa cctcttcctt aaacggtgtt
    11041 caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgaga agctattttt
    11101 gtttggctgt tcttattact tgatgcagga cagcgttttc acaacctttg attcacggtt
    11161 ttccagaagt agcttcttcg aaaaataagc cgaaatttct tgaagctgat cacttctgtc
    11221 gcaacctcta ctgactaagg cttgatataa gcataacctt gggattgcag ttcaactaaa
    11281 tcaaggacac ctgctggtac aacgattact tgttcaggta aagaactttc tgaaatatga
    11341 ttagcacgca tagcattggc gcaagcgtga aaaacgactc cttgcaaatc taagaattct
    11401 gcattggcag agtctagata accggtaatc gcgatgccat ttacagatac gacaatcgtc
    11461 gtttctggag aagcttttat caggtttttt acgtttttcc ctgtttctga ccatttttct
    11521 aattcatcaa tatggaaaac gactttcatt gaaatcattc ctttactttt taataatcat
    11581 cttgatcgct tgttctagtt tagccgcttg ttcttctgga ttactatcag gtttctcaaa
    11641 gcaatttttc agattatctg caacgatcat gcccattacg cgatcaatac tagaacgaac
    11701 agcgcttaat tgagtgatga catccataca ttctttttcg tcttcgatca ttttttggat
    11761 accacgaatt tgtccctctg tacgtttcaa acggttcagg atatttttat tatcaatctc
    11821 cataacacat tctcctctat ttttataatt agataatacc ccttatcggt attttatgca
    11881 aatataaaaa gtagtgaaaa aaatgaagag tcattctggt tgacatatta ataaagctcc
    11941 tgtttaatat accccatgag gtatttataa aaatgtaaat gaggtgcaga aaatgaattt
    12001 gctatttcaa tcaataaaat cgataacagt cccagaatta aaggaaaagc ttttggaaaa
    12061 tccagcgtta cttgatgtta gaacacctgc agaataccgg ggcgggcata taaaaggagc
    12121 taaaaatgtt ccattacaaa gtataaacag atatgatgga gacaaagaaa agaccgtcta
    12181 tgtgatttgt caatcaggga tgaggagtaa acaagcagct aaagaattaa agaaatctgg
    12241 ttatgatgta gtgaatgtac gtggtggcat gaatcaatgg tttgatcgaa cagtaggagg
    12301 gaaataagat gaaaatcgtt attgtcggag gtgtagcagg tggaatgtct gctgctacac
    12361 ggcttcgccg attaatggaa gatgcagaaa tcgttgtttt tgaaaaagga ccgtatgttt
    12421 cttttgcaaa ttgcggcttg ccttattatc tttcgggaga aatcagtgaa cgggaaaatc
    12481 ttcttgtcca aacgccagaa tcattatccg ctcgtttttg tttagatgtg cgtccaaatc
    12541 atgaagtgac agccatcttt cccgaaaaca aaacggtaga agtcgtacat gagggtcaaa
    12601 aacacattga acagtacgat gcattggttt tatctcctgg tgcaaaacca gttgttccat
    12661 cgattccagg gataacagaa gccgacaatg ttttttctat tagaaatgta ccagatatcg
    12721 ataaagtgat gcatgcatta gaaaaacagc caaagcgtgc cgtgatcgtt ggtgcaggat
    12781 tcatcggatt ggaaatggca gaaaacctaa aaagaagagg tttagaagtc atggtgatcg
    12841 aacaagcacc acatattctt ccaacactgg atgaagaaat ggcagctttt atagaaaaag
    12901 aattgtctca tcaaggagta gaagtgatta cttctcatgc tgtcgctgga tttgaagacc
    12961 acgggaaacg attgcgactg gatgatgggc gtaccatccc tgctgattta gttattttat
    13021 ccattggtgt tcgtcctgat aaccagctag cagtgaccgc tggaatcgaa ttaggcatac
    13081 gcgggggtat cctagtagac gaacgttatc aaacgaatat tcctgatatt tatgcggtgg
    13141 gggatgctat cgttgtaaaa cagcaaatca ctggaaaaga tgcacttatt tctcttgctt
    13201 caccagccaa tcgtcaaggt agacaagttg cggacacgat ttccggaatt tctcgaagaa
    13261 atcaaggcgg tattggaaca gcaattatac gaacgtttgg aatgactgcc gcatccaccg
    13321 gtttaagtga aagaacagcc aaagaaaacg aactgtcttt tgaagtcatt catgtatcag
    13381 gaaaagatca tgcaagctat tatccagaag caacagatat tttactgaag ttgatcttcc
    13441 atccagagac tggcgagatt tatggtgcac aaggtgttgg ggcaaaaggt gtggataaac
    13501 ggatcgatat tttagcaaca gcaatcaaag ggcatttgac gatcttcgat ttaccggaat
    13561 tagaattgac gtatgcaccg ccatttggct cagccaaaga tccagtaaac atgctaggat
    13621 atgcagcaat gaacattgca gaagggctta gtgaaaccgt acaatggcat gaattgccga
    13681 cagaactagc aaaaggaaaa attttattag atgtgcgaac agcagaagaa ttggaaaagg
    13741 gcaaattcaa ggaagccaaa catatccctt tgaatgaact tcgggaccga ttaaatgaat
    13801 tagacagcca acaagaatat atcgtcagct gtcatagcgg attacgtagc tatctagcgg
    13861 aaagaatctt gaagcagtct ggctaccacg taaaaaacct tgatggtgca ttttctttat
    13921 atcaaactgt ccgacctgaa gaactgatat atcctaacaa atgatactcg ttaaaaagct
    13981 tgcattcgac tcttaaaaga ggtaaactaa tcggttccac cactttaaaa aaagaggaga
    14041 atgtgtatgg tcaacacaca aaaacagtta gaagaagcat atctgaagaa cgtgtatcaa
    14101 aagctaattg ataaaaaaaa cgaactgcgg gaatcgatgg atacggcaaa aatagaagga
    14161 aaccagtcgt tgaaagaaat ggctggcgag cttcgattga attttgacag ttacttagat
    14221 aacctggata ctttttcgat gattgaaatg aaaaatagag aaatcgatca gatgaatatc
    14281 aaacagaaaa acgcagaaaa tcagctagca aagatcgaac gtttattgaa aagtccctac
    14341 tttggaaaag tcgttgtaga ctttctggat aatgagccaa aagaatcctt ttatatcggt
    14401 acagcaaatt tcacagatga tgcagatgaa aatcttgttt atgattggcg ttcaccaatt
    14461 gccgaacttt tttacaataa catgattggt ccatcttctt atacggttcg tcaaaataaa
    14521 atcgatacgg ttattaaaga aagacgacaa tttcttttgg aaagagatcg tttggtcaag
    14581 ttttttgata cgtctgtagc tattcaagat gacgtattat tagaagtctt aggccaagat
    14641 gagaccaatg aaatgaaggc tatcactgct acgatccaat cggaacaaaa cacaatcatt
    14701 cgggatgtga aaagtcaaaa tatcttggta aatggcgtag caggaagtgg taaaacctct
    14761 gctatcatgc agcgtattgc gtatctgctc tatttatatc gagatacgat gacatccgat
    14821 gacttgctga ttctatcacc taatcatcgg tttatcgact atatttcgaa tgtcctacca
    14881 tcattaggtg aaagaaatcc actgaatcta accatcatac agctggtttc acagctttca
    14941 gcagaggaaa tagaaggtga agaggcttat ttcaaacgta tcacgggaga aaatgtatcg
    15001 gagcagacag aacgtttgcg tagcaaaaag tttatcgata atctaaaaca atcagatcct
    15061 ttgtttttag atcatccgaa ttttatccgt ggacttacta aaaatggaaa aacagtcctt
    15121 tctaaaaaaa cgatcgaaaa aatatatgag aaagttcctg ctcatcctaa attgatcgat
    15181 aggcttcaag caacgaaaaa ggcactgatg agcgaatgga agaatcatct tttgaaacaa
    15241 gcaaaaagtc ctgctgtgca gaatcaggta ctctcgttga cagaagatag gcagttggaa
    15301 ttattcggta aattgatttc tgatgattcg gaacaaagta ttgtagctta tgcaaggaaa
    15361 cttttacaaa aaaaatatcg aaaaataacc agacaaatcg aagaaatggc atgggtggat
    15421 gagcatcagt tgtttgaaag aatttatgaa aaacgatatg gctcagctta tgcttggcaa
    15481 cccacacgta ctgttgatga agcagtcatt atattagcta ttcgtcattt acttgttgaa
    15541 aaggtaaatg taccagcatt tcgctatcta ttgattgatg aagtacaaga ttataccctg
    15601 gcacaattag gtctattgat cgaattattt ccaaaaactc actttacttt agtaggtgac
    15661 gagaatcagg caatctttaa ttcttccact acttttgctg atattatgcg ttgtttcgat
    15721 gactaccatc tgccgatcca tcggtatgat cttagaaata gttaccgttc tagcggagca
    15781 atcacagagc ttttcaaaac ctatgctgtg gatcaagaga agatcgacat cgtatcgatc
    15841 cgaccgcaag gaaaagaacc cgaataccgc tcttttcgat cctctgaaga attgttgaaa
    15901 atactagctg agattttggt ttctctaaaa ggagaaaaag cggcagttat cacccaaaca
    15961 gaagaggaaa cccaaacgct acaagaatcg attaaaaaaa attcatatgg attagaaaac
    16021 tgccaaatca ttcctttgag tctagcaaaa ggactggaat ttgatcatgt aatcttatat
    16081 ccatttgaaa acgatggaga tgagcaaaga agacgcagac aaatgtacac ggcgatatct
    16141 agaggaatga aaagcattgt cgtattggaa agagcaacat aattaagaga taaaaaataa
    16201 aaagaacaac tgataaaaag aacaaaatat ttttcatttg ctgatgagaa atgttttgtt
    16261 ctttttctta ggaaaaattt aggaacactt tatgtgaaaa gaaatgaaat gtatggtaaa
    16321 ctcctacgtc gtaacaaaaa aacgtgaggt gaggatatga aaaaagtaac aagagaagaa
    16381 gttgcatcaa ttttggtgaa agataagtat ttttcaaaag gtaattactg gttaaagatt
    16441 tggcaaacgt tagtggctct tttaggatgg atgatcgttg ttcttccttt tatatgggtc
    16501 tttttcccat taacgagacc agaaagagcc aaggattttt ccttgtacgc ttttttatca
    16561 gaaaagaaaa tgctgtattt cttaatcggc ttttttgtat tgattttttt cagtttcctg
    16621 attacctcat ttgtattgac taggtggaac aatcgaaatc tatacaaaaa agtaaataaa
    16681 tcttttgaat acgccgtttg tataattaag cgagacaaat aaaaaagcca tttatgttac
    16741 actccaattg taatacccgc taaagcatac aaggagagtg aacataaatg acttataccc
    16801 atcttacacc aaacgagctt gtaatgatag aagcata
//
LOCUS       DACDNO010000060        16766 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDNO010000060 DACDNO010000000
VERSION     DACDNO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.060"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..832)
                     /locus_tag="IWI22_002143"
     CDS             complement(<1..832)
                     /locus_tag="IWI22_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9169520.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1644)
                     /locus_tag="IWI22_002144"
                     /pseudo
     CDS             complement(846..1644)
                     /locus_tag="IWI22_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
     gene            complement(1677..1961)
                     /locus_tag="IWI22_002145"
     CDS             complement(1677..1961)
                     /locus_tag="IWI22_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9169521.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1958..2959)
                     /locus_tag="IWI22_002146"
     CDS             complement(1958..2959)
                     /locus_tag="IWI22_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9169522.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2961..3863)
                     /gene="gnd"
                     /locus_tag="IWI22_002147"
     CDS             complement(2961..3863)
                     /gene="gnd"
                     /locus_tag="IWI22_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9169523.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            4027..4875
                     /locus_tag="IWI22_002148"
     CDS             4027..4875
                     /locus_tag="IWI22_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9169524.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5521..5727
                     /locus_tag="IWI22_002149"
     CDS             5521..5727
                     /locus_tag="IWI22_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9169525.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5929..6930)
                     /locus_tag="IWI22_002150"
     CDS             complement(5929..6930)
                     /locus_tag="IWI22_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9169526.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6935..8848)
                     /locus_tag="IWI22_002151"
     CDS             complement(6935..8848)
                     /locus_tag="IWI22_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9169527.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(9016..9522)
                     /locus_tag="IWI22_002152"
     CDS             complement(9016..9522)
                     /locus_tag="IWI22_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9169528.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9682..10122
                     /locus_tag="IWI22_002153"
     CDS             9682..10122
                     /locus_tag="IWI22_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9169529.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(10148..11305)
                     /locus_tag="IWI22_002154"
     CDS             complement(10148..11305)
                     /locus_tag="IWI22_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169530.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(11308..11664)
                     /locus_tag="IWI22_002155"
     CDS             complement(11308..11664)
                     /locus_tag="IWI22_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169531.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(11962..12855)
                     /gene="sgrA"
                     /locus_tag="IWI22_002156"
     CDS             complement(11962..12855)
                     /gene="sgrA"
                     /locus_tag="IWI22_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9169532.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTGKVNNELDLVPII
                     PTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWM
                     GVLLILIVGASYFRSLFHRVK"
     gene            complement(13051..13890)
                     /locus_tag="IWI22_002157"
     CDS             complement(13051..13890)
                     /locus_tag="IWI22_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9169533.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            14075..14962
                     /locus_tag="IWI22_002158"
     CDS             14075..14962
                     /locus_tag="IWI22_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9169534.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(15546..16241)
                     /locus_tag="IWI22_002159"
     CDS             complement(15546..16241)
                     /locus_tag="IWI22_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9169535.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGAYYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(16225..16623)
                     /locus_tag="IWI22_002160"
     CDS             complement(16225..16623)
                     /locus_tag="IWI22_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9169536.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         5025 a         3395 c         2582 g         5764 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgccg catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat cctgttataa aaaaagcagc
     1501 ttgtctgtaa aagtgttctg ttttcccttc tgcttgtcca ttctccttat acatgacagc
     1561 aagaggtgtc catggagcac aattactagc taaagtaggg atgactccga aatcgattct
     1621 tgcaagattg gcactataac ccactagatc ggtcagtttt cctccgccaa cgccaactac
     1681 gaaatcgatt tcattttttt gatgattgta gctattcgtc tggcttcagc ataactacat
     1741 tctccagaat aacgatgata aaaaaattga atcgtttcgt cattcaaaaa tactaattta
     1801 ggtagcgcct tttcccatga taccgttccg tgggtgacca gtactctttt cgcgccgtat
     1861 gatttcaaga gatcagaaac ttttgaaata ccaccttctt catataaata aaattgaggg
     1921 cctgttttca cttttaaacc gttcaccatt ttcttcatca tccttttata tagttgctct
     1981 ttgtctggac agaacgttca ggaagaaggt gctgactgat ctgttcgaca tctctgacac
     2041 atttttcacc cattgcttct aaacattccc ttgtgtaggc cgatatatgt ggcgtaatca
     2101 ctacttgttc aaacgctaaa tacggatgat cttgacgtcc cggttcttct tctaaaacat
     2161 ctgtggcata tcctgcgatc tttccgcttt tcagaccatt aatcatcgct tcttcatcaa
     2221 ctagagcgcc tcttgcacta ttagatagat aaacattttc tttcatctga tcgacttctt
     2281 tatcagaaat catgtgatag ttctcttctg tcaaattcgc acataaacaa atgatatctg
     2341 attgttccag taattcttga agttctactt tttttgcacc aaagctttcg atgtataaat
     2401 ctgatttgta aggatcataa gctagaacat cacagcgaaa accgttacgc aaaatttcga
     2461 ttactccgct tccggtattt cctgcaccaa tcacaccaac tgttttgtta aacagcgtcc
     2521 taccgacgaa ctccgcccga tcttcccatc gatcgtcctt gactcttgct tgtgcttgtg
     2581 tcgtgcgtcg aagcatggac aacaaattag ttatattatt ttctgctaca gcatttcgtt
     2641 caactaaagc aggtatgata gatacgattg tctggtgttc ttttgctgct tttaaatcaa
     2701 tattgttgta accaatacca tgtcgggaaa tcaatagcag ttcatctttg ttttcaaaaa
     2761 aatctcgtcg aaaaaatggc gtaacacttg caataatgat attgaatcct ttaagtaact
     2821 ccgctagttc ttttcctgga atctcttgat caacagtaaa gaaatgaacg tctccaactg
     2881 actttaatct ttccaagtgg tgtggaaaga ctcttccgaa actgctggaa ttaacgattg
     2941 ctatatgata atctggcatt ttactcaccc ttttcaacta tttcatgtcc gccaaaccca
     3001 tttcttagtg ctgaaactac ttttgctgaa aaactatctt cttcctgtga aagattcctc
     3061 atgaacaaac tagttgcaat tactggaact ggaacagcca tttctagcgc tgtttcaacc
     3121 gtccattttg cttcacctga tgcagaaacg attccccgat aattacttaa attgggatgt
     3181 tcgtttaact gatcttccat gatttccatc agccagccgc gaatcaccga accatgattc
     3241 caaacacgtg cgacttgttc caagtcaaaa gcatactcac ttttttgtac aagttggaat
     3301 ccttcaccga ttgcttgcat cattccatat tcgattccat tatggatcat tttcaagaag
     3361 tgtccgcttc cggatgcacc agcatacaga tatcctcttt caattgctac atctttgaat
     3421 aattgttcca cggatagaaa agtttcttta tctcctccaa tcattaagca ggcttgatgt
     3481 cgtgctcctt cgattcctcc agaagtccca caatccaagt aaccgatttc atttttctga
     3541 aactcttctg ctcgacggat tgaatcttta taataagagt ttccgccttc aataataatg
     3601 tctcctggtc cgagttttgg aaaaagtttt cttaacatgt tttcggttat ctctccagca
     3661 ggaagcatta accagatgat ttttttctgc tcaaaagtag ctagaaattc agagatctct
     3721 tgataagcag gaatgtcatt ttctcttgcc tgactaacag ctgtacttga aatgtcgagt
     3781 ccttgtactt tgtgatggtg ctcttttaag tttaaagcta aattcaatcc catttttccc
     3841 aaaccaatta caccaatttc catgatagcg cctccatttc ttataccctc attatacaaa
     3901 aaaatttttt tgtaaataga ttttacaaaa atattttttg tttgattttt tcctactctc
     3961 cttgtatctt taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga
     4021 attctcatga gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca
     4081 aaagaacagg ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct
     4141 attagcaatt tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa
     4201 aaacttggtt ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat
     4261 ctatctgcta tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg
     4321 caaaaggtgt tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa
     4381 aaggatctaa aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt
     4441 gttggcggtt cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc
     4501 gcaacttctc acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa
     4561 aaagactgtg ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct
     4621 gaaaccgtaa gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc
     4681 cttgctaaac ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct
     4741 gaagctttag cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg
     4801 atgttttaca acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct
     4861 gaaatcaaag aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca
     4921 ctaccaaata tttggtagtg gcttttttgg ttctgcaagg gtggtacaga acatcaatag
     4981 tcggtgggtt agaacgtatg tcatcgcatc tttggagaag gttgtattgg tattattcga
     5041 gttacttgtt agatgcgata acaaagttag tatacagtag taaaaaattt atcttttctt
     5101 ttttttatta tttttgtttc ttattttcta aaaaattaaa aaaaattttg gtgaattaag
     5161 tatttccttg ataacactgg cttttggcct cattttaggt tatcatttgt aattttattt
     5221 cacagttgat atgattgtta tgtaaaataa aaaagtttag gatacatcga atttttgggg
     5281 aaattctttt gggggtatcc taaatctttt ctattatatt cccatatttt ctattattgg
     5341 tctagacgta tgatggtaaa ctttgtacct atctgattgc tgaatcatta attgcttatc
     5401 attagccatc tttgtttcaa gttcgctaga tgatcatttt attcacatcc taacaaactt
     5461 gtcacattta tagattctta atctatttat catacaatta taaaaaagga ggtgctgctt
     5521 atgaaagcta tccatatttc tttgcttttt ttcgctagtg cattgatttt atttgctatt
     5581 catctttgtt cccagcctta tatcaataac aacggaatat tagtggaacc agcattcttt
     5641 tgcttaccac tcggcttttt gtcccttctt gcctccgcag gcttcggatt tgttgctttt
     5701 tttaaacgat caaaacaaca aatctgactt ataaaattta cgtaaaagag atcgtgacaa
     5761 taatcgtgtg atattcccaa atttcgaata aaaggtgccc cttgccatct ctaactcctc
     5821 agtcacaagt tacaatattc ataaaccatg aaaagcttgt ttattgaaat tgctccttgt
     5881 gacttggagc taagtgacag attacaacct cttttgcatc ataagacttt actccttgaa
     5941 aaacattact aacttttgat atgtttcttg cgcttgttta ttgctgaaat caaactgata
     6001 ttcatggttc aaaacagcat ttttctccgc ataatctact tttgtgactt tcacccccgc
     6061 tttctcaaat acagaagtta cttcttccat ttgtttcgta aacgtatttg tatttccatc
     6121 cgttaagaat gtgttttgcg tccaacgatc cgcatgtctg accaaggcta aacttttgac
     6181 cttctctgat tgttcaaagt ccttattatc aaagtaagcc catccacatt tttcataaag
     6241 aaagctagag attttatcac ttacttgagt aagtgcagaa ggatccaaca gaccagacaa
     6301 agaaataaac ttcgtaatct gcttgttctc caaagtcgga tcaagatgat cagctttttt
     6361 aatttcttca ttttgaagtg ctaacaaata ttctccacta atttgtgcac ctgccgaatt
     6421 tccaccaatc gaaatattcg accaatctgc ttgatctttt atctcttttt tagctgcttg
     6481 aatggcttcg ttcaattgga taactggtgt aggatgtttt gcttccggtg atcgttcata
     6541 atctatattt acgatgattt gctgcgtgtc tgaacataga tattccaaat aatcacgaca
     6601 atcattttta tcccctccta catatgcgcc tccatgcatc cagaaaagaa tcggtaattt
     6661 tttcttttcc actttcggtg tgtagatatc catattatta tttttgtact ttgaaggata
     6721 ggatatttct ttttttatat cgatttgttc gattttttgc tggaaatcat ctggtcgggc
     6781 atatacttgt tctttttcac ttgtgtcaaa ttgttttcta acaagcaagc taatcggctt
     6841 tggtgtgatt tgattaatca aaaatacagt gactaaaata actacgatga taactggtac
     6901 tgcaatcact attcgcttga accatttcat tcatttaccc tctctttctt cgccaaatcc
     6961 atgtgataat cgtgctaaca gataaaaatc cacctgaata aatgagaagc gaatgtttct
     7021 tttctccagt tttcggcaac attctttttt ttgcatctgt ctttttttgt actttcactg
     7081 tcatctgaat cggttgcgta tcatcacttg attgatcctc tcgaggttca ttctttccct
     7141 tattgtctgc attctctgtc ggatcttgct tttttggtgg agctactggg atttttttgt
     7201 aatagaaaat aatagttggt atcgtctcat taaaaatccc ttcagtcggt ccttcaacat
     7261 ttgctaattc gtatccttcg aatgtctttt gctctgtcat ataattttct ccataagctc
     7321 cgtaaagctg gatttcgtcc tggtctttga gtagactttt attgttttca tccacatact
     7381 ttacgtttat ctgatcatgg aacactactt tcaaaaagtc cgttacatgt ttcgtgtagg
     7441 cagcatggtc aacttcctgt gcctttccat gccctgctcc ttctactaat aaaagatctt
     7501 tataccctcc tgataactca tataattttt gcgcgttaga cacaggtact acatcatctg
     7561 ctgttccatg gataatcaat ttaggcaatg aatctgacat tccaatcgta ggtagagcag
     7621 cttttgtatc caaatgcatc tcctgatcaa aatagtattg tttcaaaagg cccataaata
     7681 aatcttcatg ctcttgatcc ccaatgatgt ctaactgatt ttttatggca tccggtagtg
     7741 cgggggctac tgtctggctg tacgtaaaac gtagttgttc atcaattgat gcaaatccgc
     7801 aattttcaat aattcctttg acattgacag gaatatcttt cgctaataca gacatcatcg
     7861 tagctgctcc catcgaacca ccataaagta aaatcttttg agatggccgc tctcgaactt
     7921 cttgatttat ccagtaaagg acgtcatctg actcatattg tccataggtc acataatcgc
     7981 ctccgctatt accagtcgct ctggaatcaa caaataataa attatacccg gccttataaa
     8041 aatcattgta ttcttctgta acgatcccgt tattccaatt ccctcgaaat ccgccatgga
     8101 tcatgactgt tttatctgaa ccttgatcga cataatatgc atatacatcc atcattttgc
     8161 catcttgttt gtttagtaca gtttgatgaa gttgttttgt atttggatag aagcctgtgt
     8221 tttctgtagg cgttgtccca tcatctgtac ctccaagcat cgacatcaac acactgtttt
     8281 ttgcaaaagc gatatcgtac aatagataag tagcaatcag atgtttgata atctctttat
     8341 ctacaccata atcattaaca gcaagctcag tgactaataa ctctattaat tttgacttga
     8401 tgtcttctgt tggtccatta agtatttctg tcatctgttt accgaagtaa ttcaaatatt
     8461 ctttaccatt gaaattttca cctttaccga tatcatcatt ttctgtccca ttctctttgt
     8521 cacttacttt agcagaagag gattcgtcgt ttgacatttt tgtagctgtt gcttcttctg
     8581 tttgttggtt atctgtggta gcggtcgttt cttgatctgt gctagtaaca gatgtctcaa
     8641 ccgttgtttc agtatttgtt tctgtttgtg aagtactcga tttcgtttca tttccagcat
     8701 cagtagtttc tgtggtttct acctcccgag attccgattc ggaagaaatg ctctctccat
     8761 atactgtcca ttgcgggacg cttgtactta cagcaaataa gatgaccaac gctcccttta
     8821 atacttgttt tgttctcaag ctattcactc ctttttatgt ttctaggaga attatatgta
     8881 aacgcaaaca ttttttatac aaaaaataaa tggtttatcc cataaaaata gaacaggatt
     8941 attttctcat tttacgtaat aaagcagaat ggataaacca gtatccattc tgcttttttt
     9001 aaacgaagaa acaacttatt gctccaaaaa tgtcagaaat cgtttatccc aaattttctc
     9061 gtaaaaagcg attgtttcct ttgctttcat aaaaggatta tcataagttg tcgttgctcc
     9121 tctacacatc ataagctggt atcctaatcc atgagcaaac ttcacagtcg tatctataca
     9181 atactctgct tgtgccccac ataattcgat cgaatgacag tccaattcag ttaataaaac
     9241 actcaaatta gtcttataaa atgaattcgc atgggttttt ctcacaaaat aactgtgctc
     9301 aggaatagat aattccgaca atagctccca tggttttgtt tgtggcacta attgagcatc
     9361 ttcgtgttga acgaaaataa ctggtttccc aatcgtttcg tatgttttga ttcttttatt
     9421 cactaatgct gtcactgttt ctaattcgtg tatcggcgaa gaatcaaaac ataccccttt
     9481 ttgtaaatcg atcactacta ttgcgtcggc tgtgtttgac atacttgtgc cttctttctt
     9541 ttttcttgat ttaatagtat ttcttatcta gctagttcca ttatacgtga caacattttc
     9601 ttattttaca cctctaaatg gcacttttcg actatttctt catgttatta tttcataaat
     9661 acttagctat tggaggaatg tatgatgatt aaattgtata caggttctgg ctgctcgtct
     9721 tcaagacgcg caaaacgttg gcttatcgtc catgatatac cgtttatcga acagaactta
     9781 attcgtcaac cactaaccaa aaatgaattt atccacttac taaaattgac aaacaacggc
     9841 gtgaccgaaa tgctctctac tcattctatt agttataaaa atcttgttca cagaatagat
     9901 gaactcagtt tgagtgaact atatattgag attcgtacat ccccttcttt attgaaaaaa
     9961 ccgattatct acgaccaatc tgcaaaactt ttgactggat ttagcgaaga taaaatcagg
    10021 atgttccttt ctaaaactaa aagaaagcaa gagctcaacg aactcttgct ttctggtcat
    10081 cttgtcgatt ttaaagaaaa taatcttttt gcaatgtctt aattttggca taaacaatct
    10141 gcttttctta attaacgaaa tccaattttt ccaatatgga tctatttttc tcattcctga
    10201 tttttatgat ttcagctttc agacgcgccc aatcattttt tgtaggttgt ccattccagt
    10261 aaaatatctg tgtattcgtt tgatcatatg cctttttgtc gataggataa tcgctaatga
    10321 caatatcagg taactgatca gcggatgcac tatattttat tgggcgttca gtcatagata
    10381 atatcctttt ctttgcccat aattcctgtg ttttaccata ctttgattga atatagaata
    10441 tgacaggttg ttcgtgtttt ataaaaatat ccctgactaa catacagtat tgaaaaatca
    10501 accgtctgtt cttttcataa taagtattaa gctctggaat tctgcgagct accccactaa
    10561 aaaactgttg catgattgga tacatcactg gaaatatatg ctgaaaatct ctgtcttctg
    10621 ctgattttcc aaaagcatcc accttctttt ttgtcccaaa aacccttgaa tacaaatgga
    10681 tcattgccaa attgacatat agataatttt tttctgaagt tgtaaaacaa atcttgaagt
    10741 ttttttcaaa attttctaca atagacgatt caatccagcc ccaactttct tgtactttat
    10801 ctggagtatc caacaaatct tcttccaaat aggtgttcat tacaccaaac ataaaataaa
    10861 ggtaagcttg ctctgttgga gtgatgtgat tcatcgacca gaagtttgct tcaaaaaaag
    10921 gctctagtat gtaatgcgta aactcttctt ttgttatcaa tggatgcaca aatttgttca
    10981 tctcttctgg gacatacgta atcgagtggc cttgtttcat tcgttgcaac gaaattctta
    11041 gtaggctgat ccatttcttt ctatctatac tacgcgtata tggcgcatgt ttcgcaaatt
    11101 tttctaatag ttgacgttta ttttttatac ttgataatat atcagaatga ttttcgtcga
    11161 atgactgcca atacatcaag tgaaaaaagt atcggatacg gtattcttct ccttctatcg
    11221 gattaccttt tttcaagctg caagataaat gatagttttt caaaatagta ttaagttctc
    11281 ttaattttct ttgaatcgta cgcaaactta tataattttt tgactaagat tttctaagga
    11341 ttgataagta cctttgtaaa gtgaatccag taaaatataa ccaatcgttc cctctaaaag
    11401 tatagaataa actttactca gtaaaaactg agaagaaaac tcgactttta tcatcttgtt
    11461 ttctgaaaga aaagataatt tatattctgg atacctttcg caaaacgatt ctgcttgttg
    11521 aatcaccgaa ttcatcttag ccagggtgta gtttagatct aaagcgatat ccttcaccac
    11581 atataatttc tcgtctcttt cataacaata gttcaaaaat tgatatactt gcctttcatt
    11641 gatatcaata aattcttcaa acaaagtaca tactcctttt tatctattat ttagacttat
    11701 tccgacagct acatgaataa cgtacaaata gattcgttac tgatttttca gagatactac
    11761 catgcgcagt gtgcattgtc aattattctt gctcttattt tccatttgtg ttcctgttat
    11821 ttcctgtttc tcaaatatgt tagtaaacaa aaatagatct ggcaaagcta caacttcgcc
    11881 aggcaataca gcactctcga atgaaatatt cactcaaaaa tctatcgttt tattcaattt
    11941 tagatctgtt gcttttattc tttatttcac tcgatggaag agagaacgaa aatagcttgc
    12001 tcctacaatc aaaatgagta acacacccat ccatgtaatt agcttgttca aggttctact
    12061 accagtttct ggtaaatcgc tttttctatt atctgtttct ttcgcttttt cttgatgatc
    12121 aattccacga gaaacaaaat caggctgttt tagcacactt tttaggttgc tccctgcatt
    12181 tctcttttgt tccttagttg gtatgatagg taccaaatca agttcattat ttacttttcc
    12241 tgtgctgctt tcgctgctcg tgcttcctgt gctactttca ctactcgtac tctccgtact
    12301 gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc gtactctctg tactgctttc
    12361 accgctcgtg ttttctgtac tgctttcact acttgtgcta tccgtactgc ttggcggtcc
    12421 aacaactgtc caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc catcataaaa
    12481 aacaatcaaa tcaactttat ctttactctt gatctcatca atcgatagtt ctgcagaaat
    12541 cattattgta tactcaatac cagcggtcca ttgtacgtct tttaaataaa cttccccaat
    12601 aactttttct gaacttccat taccatccca accatatgtt agaacagcat gtacatcact
    12661 agcattcacg cttttgtttg gtttgactgg aatagtaatg acttgttgtg tcatgtcata
    12721 aacaatccga tcagttttca cagtaaatga aagattctca tttgcccgtt catttgcaga
    12781 agtactgatt ccaatcattg ctgcaattat actcagccca agaataacaa ataatacggt
    12841 cgctgttttc ttcatttcta tcactctcca tataattacc ttctatttga taaaggaatt
    12901 atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac aaaagatgtc
    12961 gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt aaaaaagcaa
    13021 gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt atttcttcat
    13081 ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca agaataatgt
    13141 agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat tttgcgattg
    13201 gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac ccaaaatagc
    13261 tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac ttcctgtact
    13321 gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg ccagaaatgt
    13381 catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa tcattgctat
    13441 taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa gctcttctga
    13501 tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt ttgcgtttcc
    13561 tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca gaaacactcc
    13621 aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa cagaaacacc
    13681 ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta gtaaaaaaac
    13741 agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc caataaacat
    13801 atccgcaagt gcattattga caagaattcc taaaacaaaa ccaactaaga ctattaaaac
    13861 agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat taagtttaac
    13921 aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat agagagaata
    13981 gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac tgtctcccta
    14041 attcttctgt tagactgttt ttaataagga aaggatggga aattttttga aaaaacaact
    14101 actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta gcaagtccaa
    14161 tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt atgataaccc
    14221 aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca aagcatttga
    14281 tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata atgaacaaat
    14341 caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa cagaagagac
    14401 atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag ccaatgtaaa
    14461 attaacagat aaagatctag acactgcttg gaaagaattc tatccagaag ttagtacaca
    14521 gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag caaatgaagg
    14581 ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa aggaaactga
    14641 tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa aaaaagcagc
    14701 atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc caacgactta
    14761 tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt ctaataaaga
    14821 caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg atacggaatt
    14881 catggataaa acaatccgta aagtgatgaa aaaagacaat gtaactatta aagatcctta
    14941 tgtaaaaaat atattcaaat aaacaaattt gacccaattg gggatgtggc aaaatttgag
    15001 aagtgatttt tggaaattcc ttcttatttt tgaggcaaac cacttttgcc acaacttcat
    15061 cggggccttt ttttatttgt accaaaacaa atcactggat ttgttacaat catgaggata
    15121 taatttaaaa aagtttttta tttctatcac atgaatcact gcgccattaa gtagattctt
    15181 tcatgcttcc ctattttttc acttgccgta tcgcttcaaa aacaactatc ataaactcaa
    15241 actcgactga aaagttattt taaaaaagac taaaaatatc ccttctggac ttgttcttct
    15301 tattctgtta tactagagaa acacgaaaga tatttttatg cgacactttt catttgtttt
    15361 tatgaaattt attcaaaact ggcgtaactt cagagttaca aggatgtatt agaggttagg
    15421 gaatacatcg tttaaatgag aaataagtaa tagtcaccca aactttaaat taatgaatat
    15481 tgaatcgtca gatagcctcc tcgagttttc gagaaggcta tcgtttattt tatggagaaa
    15541 ttgattcaga agtaatcagc gacctttttt agttcaccaa tcgtttctgc ttggaaagtt
    15601 tgcacccaat cttcttggaa gcccctcact gctttatcaa tcgagggcat ctctaatact
    15661 tgatctagta gctctttacc taacgttatc gcatgagctc cagatcgaat ggctgtagtg
    15721 acttgttcac aatttttgaa actagctgct aatatttttg ttggtctttg cgtacgttcg
    15781 atctcagtcc gcaacgaatc aatcactttc atagcatcaa tgccttgatt ttgcatcctg
    15841 ttataataag gtgcaatata atatgctcca ttattaatag ctaaataccc ttgtaattct
    15901 gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt tcaacttttt gatcgcttga
    15961 aggcctgctt gattcaccgg caccttgata aacgtatctt ttcccagttc ttctatgata
    16021 actcgagcat cctctatcat ttcttcttcc gttcttccta ttgtctgaat atggatagga
    16081 atctgtccga ttattttttt gatttctttc atatgcgaga agaagtttga cgctccttct
    16141 tttttcagaa ttgtaggatt tgttgttacg cctgttaaat tcaaaaactg ttgacaagaa
    16201 taaatacttt ttgtatttgc tgtatcaagc atgatttcca tatagctttc ccccattctg
    16261 atcctcttga tcgattttaa tgattgtccc ctttcttaaa tgcggcattt ccttatcttc
    16321 tacatacatt gttcctggaa gttccggctc atgagatcca tcaaatttta tcgtgatatg
    16381 tcctaattca ttcaaattag tttggacaac atctcctact gctgtgatta catacatttg
    16441 tttatcaaaa cagagcttca tatcacaagt tatttctttt cttacctgat tcaattcaat
    16501 cttataacaa taatctgtta atgtttccgg tgcttcatct ccaaataaaa tcattatttt
    16561 ttctttcctg aatacttctg cttctatacc aacatctgtt acaaccgttt caaagatcgt
    16621 catctctgtt cctcctcgta aaatatcatt tgtctaatag tttttattca aaatgtttct
    16681 agattataaa atttattgaa taacacattc ctaacggaat ttgtttcgat attgtgtgat
    16741 ttctcagagt acaatggatg tatgga
//
LOCUS       DACDNO010000061        16710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDNO010000061 DACDNO010000000
VERSION     DACDNO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.061"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..107)
                     /locus_tag="IWI22_002161"
     rRNA            complement(<1..107)
                     /locus_tag="IWI22_002161"
                     /product="16S ribosomal RNA"
     gene            26..169
                     /locus_tag="IWI22_002162"
     CDS             26..169
                     /locus_tag="IWI22_002162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169537.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(629..1939)
                     /locus_tag="IWI22_002163"
     CDS             complement(629..1939)
                     /locus_tag="IWI22_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9169538.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1951..3369)
                     /locus_tag="IWI22_002164"
     CDS             complement(1951..3369)
                     /locus_tag="IWI22_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9169539.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3609..5177)
                     /locus_tag="IWI22_002165"
     CDS             complement(3609..5177)
                     /locus_tag="IWI22_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9169540.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5181..5591)
                     /locus_tag="IWI22_002166"
     CDS             complement(5181..5591)
                     /locus_tag="IWI22_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9169541.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5588..6511)
                     /locus_tag="IWI22_002167"
     CDS             complement(5588..6511)
                     /locus_tag="IWI22_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9169542.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6508..7386)
                     /locus_tag="IWI22_002168"
     CDS             complement(6508..7386)
                     /locus_tag="IWI22_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9169543.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7373..8710)
                     /locus_tag="IWI22_002169"
     CDS             complement(7373..8710)
                     /locus_tag="IWI22_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9169544.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8713..9816)
                     /locus_tag="IWI22_002170"
     CDS             complement(8713..9816)
                     /locus_tag="IWI22_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9169545.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9890..11398)
                     /locus_tag="IWI22_002171"
     CDS             complement(9890..11398)
                     /locus_tag="IWI22_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9169546.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11364..12056)
                     /locus_tag="IWI22_002172"
     CDS             complement(11364..12056)
                     /locus_tag="IWI22_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9169547.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(12078..13052)
                     /locus_tag="IWI22_002173"
     CDS             complement(12078..13052)
                     /locus_tag="IWI22_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9169548.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13352..14224
                     /locus_tag="IWI22_002174"
     CDS             13352..14224
                     /locus_tag="IWI22_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9169549.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14682..16562)
                     /locus_tag="IWI22_002175"
     CDS             complement(14682..16562)
                     /locus_tag="IWI22_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9169550.1"
                     /translation="MDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNL
                     FWQKKNKGIEMVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFT
                     TQTVKNKEQPTVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMI
                     RNLRIGLVAVSLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQS
                     VSRIIGEKEALSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYT
                     ALVQIDLLEDSHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDA
                     EASKKVMEYCRLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGP
                     SSIIFYQEIVPAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNP
                     LSREVVSVRLINELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLP
                     ITECSQAITDHEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGE
                     KFGDFVQNHRLEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKK
                     YL"
BASE COUNT         4905 a         3505 c         2733 g         5567 t
ORIGIN      
        1 tctttttccg gtggagcaag ctccggtgga aaaagaagcg ttcgacttgc atgtattagg
       61 cacgccgcca gcgttcgtcc tgagccagga tcaaactctc ataaaaagtt cgaacaatct
      121 tgcgattgtt aagctcaatt tgtttgctag catattgctt gcttgttaaa aatgtttatt
      181 gtcttgaatt gaatcaagac gccctacaca tttggtttgt cttactttgt tcagttttca
      241 aaggtctact tcatcgagag tgtttcccga ttcatttttg gctgccgtag caacttttat
      301 atcatatcag agagacaaac gattgtcaac tcttttttaa aagttttttt cgatttcgtt
      361 tttcgaaatc tttcagcaac tcatttatca taacttatcg tttgttattt gtcaagaact
      421 tttttgaagt ttttttcgat agaatcaagc ttcgcaagtg gttcttttta acgacttagt
      481 tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat aaaatgtttc
      541 aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc aagctaaaat
      601 attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg tttgcagctg
      661 tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt actgcttcga
      721 ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct tctataggct
      781 tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct ggagaaaggc
      841 atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt gattcaggta
      901 tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg ctattttttc
      961 tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca tcaatcatat
     1021 tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa gatttcattc
     1081 gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca ctaactccag
     1141 cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat ctagcaacct
     1201 cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca gacttaatag
     1261 caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt gttgaagaat
     1321 caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact ttttctacta
     1381 atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga aaaatcaaat
     1441 cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt ttaatagcat
     1501 aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg cttttttgat
     1561 cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt ccttttcctt
     1621 ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat ttcatttcta
     1681 cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc cccttgagga
     1741 ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct ccgactaaat
     1801 aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg attgcttttt
     1861 gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa gcattgataa
     1921 tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt tcaattcttc
     1981 accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa tgattgcgga
     2041 tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt cttgtactaa
     2101 aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg ccagcataaa
     2161 tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat ttactccata
     2221 tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca cgtcagtaat
     2281 gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa ataaaagaat
     2341 cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca tacatgaaga
     2401 agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca gctttgtagc
     2461 aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag acattgcttg
     2521 tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta aaatctgatt
     2581 gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag ctaacaaacc
     2641 ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc tttcagtagt
     2701 caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga ttgaaatcca
     2761 aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat acattaaaat
     2821 cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc ggaacgtgga
     2881 caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc catacgggat
     2941 attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat aagcaatctt
     3001 caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca ctaagcaaaa
     3061 tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta actctgagac
     3121 atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg aaatcaagaa
     3181 taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa tcatcaagaa
     3241 actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt agcctatctt
     3301 atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa cagcattttg
     3361 tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt ataactcttt
     3421 ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga cagataaaaa
     3481 acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta cataacaata
     3541 tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc atttattaaa
     3601 gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc agcataactt
     3661 cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt aaaccctcct
     3721 gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt tgatacgtct
     3781 ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt cttcttttct
     3841 gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc tatcatacgt
     3901 aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt ggattcattt
     3961 aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc aaatgctgtc
     4021 tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc gaaggtagat
     4081 tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt atcacaccaa
     4141 tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc ttcaaatgta
     4201 tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat cattatttct
     4261 tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt ccaatacact
     4321 tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct ggtacaaaga
     4381 tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc tgtacattgc
     4441 aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc aagctcgtaa
     4501 ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc tgtaccgcct
     4561 tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc ctccgttttc
     4621 gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt cttctctgca
     4681 tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg ataatcccat
     4741 agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga aggatcaaag
     4801 ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga tgcagtgatc
     4861 aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt gtaaacaaga
     4921 taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt tgtaggattt
     4981 ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt gactaaattt
     5041 ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat aatgtaatac
     5101 cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat aggatttttc
     5161 actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata taaactgaat
     5221 gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt gccaatcaac
     5281 aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt tcctttagca
     5341 gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac ggcaaacaga
     5401 ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga atgatctaca
     5461 gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa agtaactgct
     5521 aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa tagttcccat
     5581 tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt cttttgatcg
     5641 atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt ttcttgtcat
     5701 cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct tcaaataaga
     5761 catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca ggttctgcag
     5821 tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta gatgctgctg
     5881 tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga aaaacggcat
     5941 ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca taggtctgac
     6001 gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct ttttgcaaat
     6061 caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag ttttttaaag
     6121 atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct cctggacctg
     6181 ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag aaaaaagtag
     6241 cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca aaacgattac
     6301 cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg ctttctatat
     6361 attcatcata tcttccttct tttactatca agacctttgg atggctatcc ggcaggcctg
     6421 ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct atagcattct
     6481 ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt gtttccaaat
     6541 atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag aaaagttttc
     6601 tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca gtacgccttc
     6661 gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg gagcgatttt
     6721 ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt aataaaaccc
     6781 atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg ccgctagatc
     6841 aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata agcctcccat
     6901 agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct ccaaataatc
     6961 gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg ttaataaaaa
     7021 ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc aagcatcaat
     7081 cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag aagctaagta
     7141 aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt gagaatgcag
     7201 gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg ctggtactgt
     7261 ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt gtttttctcg
     7321 gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa aatcattcaa
     7381 ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat tgatttgatt
     7441 ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt ccctcgtgat
     7501 tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa aatccttgtc
     7561 tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca tcttgggcca
     7621 tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct aatccataaa
     7681 taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg atccttctga
     7741 atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac ggttctttcc
     7801 ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca ttatccatga
     7861 ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta gatttcaatc
     7921 gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta cgaatactac
     7981 cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg caatttgtga
     8041 tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc ttgattgcaa
     8101 tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg catgattctg
     8161 gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag ttatcaaacg
     8221 ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc aatttaatat
     8281 ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga ggataagcga
     8341 gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca tctaatgtac
     8401 cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa gcataaactt
     8461 ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga tcgtcagaga
     8521 atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa aataaagctc
     8581 ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt gctgcagcat
     8641 caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca aatgtcaaaa
     8701 tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg caccaacgcc
     8761 tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac catctcgctg
     8821 atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc agccttcttt
     8881 tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt gtggaagcca
     8941 ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa caaacatact
     9001 actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta cttgatacca
     9061 tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt ctagaatttc
     9121 tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg ctgcagccat
     9181 caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt ttggatcagc
     9241 ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt ttggatcgaa
     9301 tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt tttcttcact
     9361 aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat ccaaccaaat
     9421 ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg gatggtttgc
     9481 caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg tttctttgta
     9541 caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt caacgctgta
     9601 gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta tgaaatcaaa
     9661 ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta cgacgccata
     9721 gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg ttctttccat
     9781 gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt taaaaattaa
     9841 aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat tactgtgccg
     9901 ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt gctttccagt
     9961 cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat tgcaccgtag
    10021 catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt gaatccaatg
    10081 gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga ttagagaatt
    10141 ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga gagcttgata
    10201 acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa ccatcatcat
    10261 tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca tcttcttcta
    10321 aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt acttgagatt
    10381 tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga taaccgccgc
    10441 cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga ataccttgac
    10501 ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt cttccttgtg
    10561 caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc aaatagccat
    10621 tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta tcaaattctg
    10681 gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa taagaagtga
    10741 aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa ccaactgtgt
    10801 cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact tcataaagct
    10861 catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt cgaatgacta
    10921 agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg tcgatactcg
    10981 cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt aaatattctg
    11041 atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt ttcaacatcg
    11101 caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt gttaatcttt
    11161 cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt ttttcttcga
    11221 tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt aaccaagtga
    11281 ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg ccgcatgcgg
    11341 caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct tttttcataa
    11401 attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc tgtatatgag
    11461 ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc ggaaaagaag
    11521 agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat cagataaatc
    11581 cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc gaataaaaat
    11641 gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat tgggaaaata
    11701 tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct aagataccaa
    11761 ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata tatccagctc
    11821 atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt cgtcaaagga
    11881 aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata cgatgcagga
    11941 ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa attcaaataa
    12001 accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg cagcatatct
    12061 ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc aatcttatta
    12121 gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc tgtactgaaa
    12181 cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat ttctgcaacg
    12241 cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc taatgtctga
    12301 ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa tgtaacatgc
    12361 catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa ctgtgtatcg
    12421 actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt ccagatcact
    12481 tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat tttttcaaat
    12541 cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa tacataagta
    12601 atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt ttgaatcccg
    12661 tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat tggcatcgga
    12721 aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa tgtaaagaat
    12781 gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact tcccattatt
    12841 cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg aagataccta
    12901 aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt attcgttttg
    12961 attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga tagcgtcagt
    13021 tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt gtgaatgttt
    13081 gctgcttatg acttcatgat agataactat ctgaattacg tatataatca tttttaccgt
    13141 aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa agtgtatttt
    13201 ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca cttagcatag
    13261 gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga aacagaaaca
    13321 tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta taggagaaag
    13381 aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct gtatcgttcc
    13441 ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg cgaaaaagtt
    13501 tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt ctacgccgac
    13561 catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga tcgtcagtat
    13621 gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg gtcgaggttt
    13681 cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc caacgttctt
    13741 agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc ctgtggctgt
    13801 taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg atagtttgga
    13861 agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa ttatgctgcc
    13921 attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt actggaacac
    13981 ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac agattctttt
    14041 acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg ttgatgtgat
    14101 tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc ctatgctgat
    14161 tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt ctgtcaaagg
    14221 gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct tcaagaaata
    14281 agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt atttttcgaa
    14341 gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca gccttgagcg
    14401 atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata agaagcgatt
    14461 ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag cctgtctctt
    14521 gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt tctttttgcg
    14581 tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt ctaacttctt
    14641 ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata tttttttcga
    14701 tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt ttgcggattt
    14761 cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt caaccatttt
    14821 tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc tgtttccttt
    14881 ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat cacatctaaa
    14941 gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga caaattttta
    15001 aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa ttgttctaaa
    15061 acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata aatttgtttc
    15121 atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac gagttcattg
    15181 atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc aaacaatgca
    15241 tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat ggcttcaaac
    15301 aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc agcaggaacg
    15361 atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata cagggcttct
    15421 tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt actgatgcct
    15481 atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg gcaatactcc
    15541 atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt ggcttgcatt
    15601 tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc cgtgatcatg
    15661 ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc taacagatcg
    15721 atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa ttgctgcaac
    15781 cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac aaatagcgtt
    15841 tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc tcctatgatc
    15901 cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt tgatttaaga
    15961 ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa taagtaggaa
    16021 agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat tctcagattg
    16081 cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca attattataa
    16141 tctgatttca gtactatata cgttttatct ttttctctta tcactttcag ctgattgtaa
    16201 tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt ctgagtagta
    16261 aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc accaacaact
    16321 tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc tttttcaaac
    16381 attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa taaattattt
    16441 ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag ttgactcaac
    16501 gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt attatcatcc
    16561 ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt ggagtaatct
    16621 gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc atacgtagaa
    16681 aacgaatatt caatcgttct aattttccat
//
LOCUS       DACDNO010000062        16591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDNO010000062 DACDNO010000000
VERSION     DACDNO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.062"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1479)
                     /locus_tag="IWI22_002176"
     CDS             complement(<1..1479)
                     /locus_tag="IWI22_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9169551.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFI"
     gene            complement(1476..2162)
                     /locus_tag="IWI22_002177"
     CDS             complement(1476..2162)
                     /locus_tag="IWI22_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9169552.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2355..3776)
                     /gene="gndA"
                     /locus_tag="IWI22_002178"
     CDS             complement(2355..3776)
                     /gene="gndA"
                     /locus_tag="IWI22_002178"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9169553.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(3946..4125)
                     /gene="rpmF"
                     /locus_tag="IWI22_002179"
     CDS             complement(3946..4125)
                     /gene="rpmF"
                     /locus_tag="IWI22_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9169554.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4183..4752)
                     /locus_tag="IWI22_002180"
     CDS             complement(4183..4752)
                     /locus_tag="IWI22_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9169555.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            4938..5840
                     /locus_tag="IWI22_002181"
     CDS             4938..5840
                     /locus_tag="IWI22_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9169556.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(5875..7659)
                     /gene="pyk"
                     /locus_tag="IWI22_002182"
     CDS             complement(5875..7659)
                     /gene="pyk"
                     /locus_tag="IWI22_002182"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9169557.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(7733..8695)
                     /gene="pfkA"
                     /locus_tag="IWI22_002183"
     CDS             complement(7733..8695)
                     /gene="pfkA"
                     /locus_tag="IWI22_002183"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9169558.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(8863..12177)
                     /gene="dnaE"
                     /locus_tag="IWI22_002184"
     CDS             complement(8863..12177)
                     /gene="dnaE"
                     /locus_tag="IWI22_002184"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9169559.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLIKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLIENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKDGSQIPLENLSASLPLT
                     ANGYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITNYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            12404..12589
                     /locus_tag="IWI22_002185"
     CDS             12404..12589
                     /locus_tag="IWI22_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9169560.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(12651..13847)
                     /locus_tag="IWI22_002186"
     CDS             complement(12651..13847)
                     /locus_tag="IWI22_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9169561.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSESTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFILSNIFLSLFNFRK
                     VLRSRL"
     gene            complement(14017..15453)
                     /locus_tag="IWI22_002187"
     CDS             complement(14017..15453)
                     /locus_tag="IWI22_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9169562.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNEVSVALWIVDILFIINFIILATI"
     gene            15746..16555
                     /locus_tag="IWI22_002188"
     CDS             15746..16555
                     /locus_tag="IWI22_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9169563.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         4867 a         3500 c         2915 g         5309 t
ORIGIN      
        1 aataaagatt ctaaaaattg tcccttgccc tactacactt tctgctaaga tacgtccttt
       61 atagttttca accagttgtt tagcgataga caaccccaga ccatttccac ccttcgttct
      121 agctcttgct ttatccactc gataaaaacg attgaagatc ttattaaggt cttctttggg
      181 tattccttcg ccaaagtcct gaatcgcgat ctcaaattca ttcagagtgg aagaaataga
      241 gatatggact tcttttcgtg tagttgaata ctttactgcg ttatccaata tgataatgat
      301 taattgttca aagtgattac gataaatttg cagggtcact tcttttgtca aatcatcatc
      361 taaagtgatc acatagtctg gatataatat cttgaaatta ttataaactt gataggtgac
      421 ttcttttcct ttagttgttt cattcgcata atgaacatct acttgttctg ctcttgataa
      481 gtctagcatc tcttgaacta agcttttcat acggctaatt tcttgcatgc tggcgcttaa
      541 tgattcttct aatatctcag gatcatcttt cccccaacga tttagcagac taagatgccc
      601 ttcaataatt gcaacaggtg tccttaactc atgagatacg tcttccacga attgctcttg
      661 ttgttcaata tacaaacgca tacggtcaag catctcattg aatatttcag ctaaatctgc
      721 caattcatca tttgtgtcga tttcaggcat gtggatatct gattgcggat ctttacgtat
      781 agtatccatt gtatctctga gtaccttgag tggttttaag aaataagaag ataaaaagaa
      841 acctaaaata ctacttaaga tcaaagaaac aacttctaaa acaataagag tcaataatag
      901 cttatttcga atatcataga aagaagataa ttcatagaag ctttgcgcat agccgatctt
      961 ttctcttgta cctttagaat atataggctg gatagataaa aagccagttt ttccttctag
     1021 tgtaacgatc gttggaactt tacgtgtagt ctgaatcagg tttcgactgt tttcatgggt
     1081 tttaaaaatc aagttctcgt ccaaatcata aacataaaga tccattgatg gctgccctag
     1141 ctcagctata aaggaatcga tcttcattaa gtttccttcg accagtgaag tatgatctac
     1201 gacctcatct ccgtcatttg tcgctttcgt cagattacga tacgtattta ctaatgtcag
     1261 ttcttcatcc gagttagcca aacgagaagc tacttctgaa atcgtacgct ctacattgtt
     1321 tctttctttt gccacaatca gattgataga tgatttatag gtaatgacag cgaaaattgt
     1381 aaacacaacg aatatgaaga aagaactcgc aaaggcccac ttgattgtca aggagggccc
     1441 tttcagttct cttttttccg attttttcgg ctttatcacg agcgcatcac ataacctgtt
     1501 ccgcgtacag tttggatata gctttcctcc ccgggaacgt caattttgtt gcgaagataa
     1561 cgaatatata cgtccactac gtttgtttca acttctgttt cgtatcccca cactttattt
     1621 agtaaaacgt cacgtgctaa taccacgttc acgttttcca ttaaagtaag cagcaactcg
     1681 tattctcttt ttgttagttc aattacttca tttccgcgac gtacgacacg gttttctttt
     1741 tcgatagtca aatcgcgata agtaatagta gtttgtttag ccacattttt atcgccttca
     1801 atatcgattc gacgcaacaa tgcacgaaga cgtgctaata attcttcgat agcaaatggt
     1861 ttgacgatgt agtcatctgc tccgtggtca agtccagata cacgatcgat cacagaatct
     1921 cttgcagtca tcataatgat tggtgtattt ttgacttggc gtacacgtct gcagacttct
     1981 aaaccgttca attctgggag catcagatcc aacaatatgg catcccactc attatttaat
     2041 gccgcatcta aaccagttcg gccgttgtag tggacttctg cgttataccc ttcatgcttc
     2101 aactctagct cgacaaatcg tgccaagttc ttttcatctt caatgattag aatattgctc
     2161 atttattatt ttatcctttc tattctactg gctttttaaa aactctatca ttataccgat
     2221 aatatgctta aaaatctata gtcattttat catggatagc aattttaaaa aagagctgaa
     2281 gtctactcat aacaatcgac ttcagctctt tcgtcctctt tttattttct catttcctaa
     2341 cagccgaaag actattattc ttcatggtac catgagtaat ggaagattcc ttctttgtcg
     2401 acacgttcat aggtatgtgc gccaaagtaa tcgcgttggg cttggattat atttgcaggc
     2461 agacgttctg ctctgtatga atcgaagtaa gcgatggctg atgagaaagt cggtacaggt
     2521 acgccagctt ggacagccaa ggcaacgacg tctctgactg attgctgata ttttttcgtg
     2581 atttcaacga agtaatcatc tagcaataaa ttttcgatct caggattttt gtcataggca
     2641 tccgtaattt tttgcaagaa tcgagctcgg atgatacatc cagcgcgcca gatttttgcg
     2701 atttcaccaa atggcaagtc ccaatcaaat tcttttgaag cgacacgtaa ttgagcaaat
     2761 ccttgggcat aactcatgat tttactgaaa tacaacgctt ctcggatttt ttcgaccaat
     2821 tcttttttgt ctcccgtgaa ctcaaaatcg ttcgtacgag ataaaatctt gcttgcttgt
     2881 acgcgttctt ctttataagc agaaatatag cgggcaaata cagattcagt gatcaatgga
     2941 agtggcacac ctaagtcaag cgcgctttgg cttgtccatt tacctgttcc tttattccca
     3001 gctgcatcaa ggatcacatc tactactggt ttacctgttc cttcatcgtc tttacgtgtc
     3061 agaatgtcag cagtgatttc tatcaaatag ctgtctaatt cgccttgatt ccattcttta
     3121 aagatatcag ccatttcttc tactgaaagg tttaaaatat tcttcattaa gtcataagat
     3181 tcagcaatca gttgcatatc tccatactca ataccgttgt gtaccatttt cacataatgt
     3241 ccggctccat ttgggccaat atatgtgaca catggtgctt cgtcttctgc ttttgcagaa
     3301 atttgctcta agattggtgc aactagcgca taggcttctt tttgtccacc aggcatgatt
     3361 gaaggtcctt ttaaagctcc ttcttcacca ccggaaacgc ctgtaccgat aaagttgatg
     3421 ccagagttgg ctaactcttc attacgacgc atcgtatctt taaagaatgt atttcctccg
     3481 tcaatcaaaa tatcgccttt atcaaggtga ggaagcaact cttggatcgt tgcatctgtt
     3541 gccggtcctg ctttgaccat tagcaagatc cgacgtggtt tctcgataga ttctacaaat
     3601 tcttcgattg tatatgttgc tttgaaattt ttatctgggt gttcttctac tacagcggtt
     3661 gttttagccc ctgtacgatt gaataaagcc actgaatagc cgcggctttc tatgttcaag
     3721 gctagatttt tacccataac ggccataccg acaacgccga tttgttgttt tgacatatga
     3781 actcctccta catacgggat ttctccctaa tctcttacat tatagcttaa cacggctaca
     3841 ttgcaaacta aagaacaaca tattttttat ttaccgacta caaaaaaata aacctcaagg
     3901 ctccatggtt agccttgagg ttttacattt tctaattaaa atgtattaag cttcttttga
     3961 cattacgtcc ttaccatcat agtaaccaca atttgcacat acgtgatggc tttttttcat
     4021 ttcaccacag tttggacatt cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg
     4081 ttttgctttt ttagcttttg atgttcttct agctggtact gccattttgc tacacctcct
     4141 tgtaaagtct tgtatctaaa ttagacacaa tatattccaa tcttacttgt tatcgtcgtc
     4201 ttttgattta gaatcaaaaa gttcagatag ttttgcaaga cgaggatcaa ttttattctc
     4261 ggcttcttcc atttttctgc gctggtattc ttcttcggaa acaacttccc aatcattgcc
     4321 ttttggaagt tctgttgctg cttcctcttc ttttgtcaat acccgcattg gaatcgccaa
     4381 taagatgttg tcttcaatcg actctgctaa attcaataat ggaccttcaa ttagcaaaat
     4441 ctcatccgca gacatttgtt catctctact ttggaattgt tcttctgtca tgaatacttc
     4501 gtctacagaa aaatccatgg gcagttcaac aggttctagt gaacgtgttg aaggaactgt
     4561 caaagtcgtc tgcacagtgt aatgaacgat atagcctttc ttatcaactg agaccaaacc
     4621 ttcgacttcc actggcgcaa catcaagtat taaattgtct cgcttcatga gagcggcttt
     4681 caagtcgagc gtctcatgaa aagttaacgg tgtctcctgg tattttctca attccaataa
     4741 agaccatttc atttttcatc actcctaagc aacaaaagca attatacagg gcaaaaagca
     4801 tgttgtcaat gaaattttct tgacaccttg cagttcataa aaaaacagaa aaatctttat
     4861 tagaaagcct ctgtttgcta tcatcctcag aatcatttac aataatcatt attccattaa
     4921 aggagcggat gatcttcatg aacacgaaag atatcgaata ctatgtccaa atcgtcaaac
     4981 aaaaaaattt tacgaaagtg gctaattatt tccgtgtgag ccaacctact attacatatg
     5041 cattacaacg tcttgaaaaa gagtttgaca ccgtacttat tacaagagat cgatcgcatc
     5101 acgaattaat cgtcacgcct tctggaaagc agctgtttct ccatgcacaa accattctcc
     5161 aagagatttc tactgcaaga gctgaaatca ctcagttgca aaagaaaaag cttcgttttg
     5221 gcatgcctcc tattatcggc aatgaatact ttcctaaact gtcaagttac tttatcaagc
     5281 atgaattgat gaatcaaatt gagattaccg atggaggctc tcgggactta tacgggatga
     5341 tccgacaagg gaggatcgat ttagctgtat taggctcaac acagccaatc gtggatcaat
     5401 atttagaaac agagttactt ttggacaaac gtttcatgat cgtcgtgcca cctacacatc
     5461 ctctttccaa gcaggatact gtctctttca aagatatagc agatgaacag tttgtactat
     5521 tgaacgaaca ttatgtccac cccgcagcat tcactaagct ttctaaacaa acacatactc
     5581 atccaaaagt tgtttatcaa agcaatgact tgacgatttt aaagagtatg gtccgagaaa
     5641 atgtgggtat cggttttttg gcagagattg ctgtccatcc tgaagatcat ttagtttgcc
     5701 tccctctttc agatgaagtt cagcctcgct tcttgatttc attggcaaaa cgaacacagc
     5761 agatcgtcac gcctcttcaa gaacaagtag tccaagttat ccatgaattc gttacagcac
     5821 aaaaaaacag ctcttcgtaa aagaagagct gtttttattt tttattaagc taaattagat
     5881 agctgttgtt tcgccacggt aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc
     5941 ttctgtaatt gttgttgttg cgcctttagc accaacgatt acaggaatat cttgtgcaat
     6001 tcctacaact gcagcgtgag aagtcaatcc gccttcttca acgataacag cagctgcttt
     6061 ttcgattgca ggcatatatt ctttgtcagt tgatggtaca actagaacca ttccgtcttt
     6121 tgcttttttt acagcttctt cagcagatga agcaataact gcatttgcaa cgacagattt
     6181 ttgtccaacg ccttgagctt ctaataattt agaaccaatc atttgaattt tcatcacgtt
     6241 agttgttccg cgttcaccaa ctggtacacc agcagtgatc aagattaaat cgccttctga
     6301 tgcgaaacct aattcaacag cttttttagc tgctagttca aacatttcat cagttgttga
     6361 tggtttttca gcaactgttg ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg
     6421 ttcgtcaaaa gtaacagcca agatatctgc atctggacga tattttgaaa tcatttttgc
     6481 agtgtatcct gattcagttg cagcaacgat tgttttcacg cctagatttt ttgcagcacg
     6541 agctacagat aaaccgattg tttcagttac atctgtttta tcgaattcgt tcaattggaa
     6601 tgtacctaat tctgataatg cattttcagc ttcttggtcg atacgtgcca ttgttgaaac
     6661 agcttcaact gggtagtccc cgtttgcaga ttcaccagat agcattgttg catctgtgcc
     6721 atcaaataca gcgttagcaa cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg
     6781 cattgattct aacatttgtg tagcagtgat aactgggata ccagcagcat tacatttttt
     6841 gatgatgcgt ttttgaacca ttggtactag ttcagctggg atttcaacac ccatatctcc
     6901 gcgggcaatc ataacaccgt cagaaacttt gatgatttcg tcaatgttgt cgataccttc
     6961 ttgagattcg attttaggga agatttgaac atgagtcatg tctttttctt ctaagatttc
     7021 gcggatatct agaacgtctt gtgctttacg tacaaaactt gcagcgataa agtcgatatc
     7081 gttgtctaaa ccaaaacgaa tatcgtcagc atcttttgga gtaatacctg gcaaactaat
     7141 agaaacgcct ggtgcattta cccctttacg tgatcctagc atacctgcat ttttaacttc
     7201 tacgactaat tcacggtttg cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc
     7261 atcaaataaa atgtgtccgc caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc
     7321 gattttttct tttgaaccta catgttcagg atccatagag atgcgtgttt gatctcctac
     7381 ttcaaatttg atgtatcctg cacgaccaaa atcagcttct gttgtgcctt gaacagttgt
     7441 acggatttca gctccttttg tatctaaaag aataccgaca tcttttcctg tctttttaac
     7501 tgcttcacgg accatagtca tacgtgataa ttgttcttca tggtcaccat gagaaaagtt
     7561 aaagcggaag acatttgccc cagattcaat caattgagaa ataatttcta ctgagttact
     7621 tgctggtcct aatgtactaa cgattttggt ttttttcatt taaaaaactc tcccattcaa
     7681 ttttgtttat ttaaagctgt acacagctta aatagcttgg tttctttcac tcttaaaatg
     7741 aaagtgaatt atttagatca tatagtgata agtctggttt atgtttgttg ttttcaagtg
     7801 tatcaatgat gtcagacgca acgatttcgt tgtcatggat accgacacat aatccacctt
     7861 ttccttcttg aagcaattcg acagcgtaag caccgaattt acttgccaac acgcggtcgc
     7921 gtgcgcttgg tgctccacca cggacaacgt gtcccaagat agatacacgt gtatgatagt
     7981 cgccaaattc tgaaagtttc tcagcgaatt catttccgcc cattacacct tcagccaaga
     8041 tgatcaagca atgtttcttc ccgcgatcgc ggccttcttg gattttttta gcaactgaag
     8101 ccatatcaaa atcatgttct gggatgatga tttcatccgc tccgccagcc acacctgacc
     8161 ataaagcgat gtctcctgca ttgcgtccca ttacttcgat aacgaatgta cgaacatgag
     8221 aagtcgctgt gtcacgaata cggtcaattg attctagtac tgtattgatt gctgtatcaa
     8281 aaccgattgt gaaatcagtc ccaggaatat cattatcgat tgttccagga atacctactg
     8341 ctgggaaacc gcgttttgtc aaagccattg cgccatgata agagccgtct cctccgataa
     8401 ccactagacc ttcgattccg aattttttca attgttcgat tccttttaat tgaccttctt
     8461 cagttgcaaa ttctggataa cgtgcagaat acaagaatgt accaccacgt tggatcttgt
     8521 cacctacatc tgccacatca agacgacgaa tatctccagc aactaaaccg gcaaaaccat
     8581 aattgatccc gtatacttcc agtccttcgt aaataccttt acgaacaacg gcacgaacag
     8641 ctgcattcat tccaggtgcg tctccgccac tggtcaaaat tccgatgcgt ttcataataa
     8701 acattcacct cattaaaata ctttatccta agtatgactt aggtttacaa ccatttttgc
     8761 gttaaccacg actaacattc taacattatt tttctcactt gtcttgattt acggataaaa
     8821 aaatgaaaaa aaatgaaagc aagtagtatt ttttaccgta agttatttga aaaccacatt
     8881 atcttcgcca agcagataaa taagttgccc ctttatactc ttgctgtcat ccacccaata
     8941 agcatctgac agaactactt ttcgttgcgt tttttcgtga tacataacta ctggtatata
     9001 tcctgggtgt ttctgaaaaa tcacttgtaa ttgtttcatt atctcgggat cttcccgttc
     9061 ttctgtaata cgcaaatagc aagtttgtga aggatactgg gcttctaact ggttaggatc
     9121 agcaaagctt tctccaatta gttgcagttc ctgattatat ttgctgattt cgctccgccc
     9181 ttgaacaaag atcactcgat caacggcaaa caaattacga tatttacgat aaagagttgg
     9241 aaaaactgtg acagaaattt ctccactact gtcggttcct tcaacaaagg ccatcagttc
     9301 tccttttttt gtccgaattt ctctaacttc cttgatcatc actaataaat tgactggttg
     9361 attagggacg atatctgtaa tcagctgaac ttgtttagct aaacgaagac gatcaaaccc
     9421 ttctgcagga tgacctgaca aataaatacc taagtactcc tcttccagct tcaagcgttc
     9481 ttctagtgta tagtcagcga cttcttcttc ttttaatgcc atgattccca gcaagtcaag
     9541 acttccacca ctgtaaacga tattttggat ttttccttcg agatcaagca tcaattgttt
     9601 cctgttagga gataattcat caaaaacgcc tactgctgtc agtgattgca gtaactctaa
     9661 tttcagccaa cgcttatcga tccgtatgag gaattgatcc actgactgga acgggccatt
     9721 ttgttttctt tcgtttaata tatcttctac aaaatcacgc cgaatacctt ttatcgctgt
     9781 cagtccgaat ctgatttctt ccttattgac caactcaaaa ctggaattac tttgattaat
     9841 ggacggcgag gaaagtttga gtttattttt acgtgcttct gcaatatatt ctttcaattt
     9901 tgatgtgtta tgccgaacag aattcatcag gctggtaaag aaagcacctg gatgatgggc
     9961 ttttagatac gccatttgga atcccacgaa tgaataagca aatgcatgtg accgattgaa
    10021 tccatagttt gcaaattttt cgatatattc atatacggtc gctgctgttt tttccgaata
    10081 tccctgttgt aatgctcctg atacaaaatg cgacttttct tcatctaata cgtctttttt
    10141 ctttttactc actgcccgtc gcagaatatc tgactgccct aatgtaaatc cagccatagt
    10201 cgaggcgatc tgcatgattt gttcctgata gacgatcact ccatatgtgt ttttcaaaat
    10261 cgggatcagt gaatcatctg ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa
    10321 ggtatctata ttttgcatag ggcctggtcg atacaatgca ttgactgcgg caatatcttc
    10381 gatgttttct ggacctaatc ttcgtaaaac gttccggata ccagcagatt caaattgaaa
    10441 tactcctgag gtttcgcctc gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat
    10501 ctcttttaat cggatctcat acccttctac tcgcttaaca gctttcaaag taaaatcaat
    10561 aatcgataag ttgcgtagtc ctaaaaagtc cattttcaaa agaccgattg cttctacctc
    10621 attcatcgta tactgggtca gaaaagcttc atcagaaccg cgttggagcg gaactagttc
    10681 aagtagattc ttatcgctga taactacacc tgctgcgtga gtcgaaacat gtcttggcaa
    10741 tccttctaaa atcactgcag ttttgaataa aagttggttt ttttcgcttg cctgaactaa
    10801 ttctactaat tgatgcgaag ttttataagc ttgggtcaat gtgattttca attgattggg
    10861 tattgctttt gaccagcggt ttgcttcact ttgcgataaa ccaaacactc tagctgcatc
    10921 tctaagaacc atttttgctg ccattgtgcc aaatgttgcg atttgtgcca tatgatattg
    10981 tccatacttt tctcgaacat aattcaatac ttcttcacgt cgattgtcag gaatatccaa
    11041 atcgatatct ggcatcgtaa agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata
    11101 ttctaatgga tccacatctg tgatttctaa cacataggct actaatgaac ccgcagcaga
    11161 accacgacct gcaccggtaa cgattttgtt tctatgagca aaagccatta catcccatac
    11221 gatcaagaaa taatcatcga atcccatttg atggatgaca gataactcgt agtccaaacg
    11281 ttcttggtat tcctttgtat aattagtgat tcggcgttcc acttgcgaat agcataactc
    11341 tcttaaatat tctccagctt gttttccatt aggaactgga taatgcggaa gtaatttctg
    11401 atggaactgg aattcaaatg aaatcgattc agctaaacgc tgggcgccta ttacagcttg
    11461 ggaaagaccc gctttttcga atcgtttgat catttcttgt tctgaaagaa gatagccatt
    11521 tgctgttagt ggtaaagatg cagataaatt ttctaaaggt atttggcttc catctttgat
    11581 atgtttcatc acttcaagtg caaaaagttc ttcttgttgt aaactagcga tgggatgtaa
    11641 tgcacaaagc gaataatttt cttttatcac atactgatac cattcaggca attgatcagg
    11701 cgtgttttct atcaagccga tataaaaatt tctagtaaaa acattttcaa gtaaaaactg
    11761 ccaatgaccg aaaaagttat cctctttatt gaaagcctca gaaacagaat gatttgccgg
    11821 aacaacaaac gctagatggt cggtttctgt catttctgca agttccaatt tttcttggat
    11881 catttttcgg ctggaaagtt ttatcagctg ctgataacct atttcatctt ttgcgtacgc
    11941 aagcaagcca taattttttt cttcataagt ataactaaga tgtaatccaa tgattggctg
    12001 tactccttct tctacacaaa tacgatagaa ttcaggtgca ccgtataaga catctttatc
    12061 cgttatggct aatgaagtgt agccttgttt ttttgcttct tgtacatatt ctcgaatacg
    12121 tatcgtactt tgtaaaagag aataagaagt aattgtataa agttgtggga aaaacatctt
    12181 gcattccgcc ttttttttcg ttaaaattca ctagcgttaa aaggtttgat ccttttactt
    12241 tcattaagat agcaggcgct gaagttgatt attccacttc atttgcttgt aatctgtagg
    12301 aatgagttct ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc
    12361 aaagaaaatc tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac
    12421 attattctgg gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag
    12481 cggaacatat gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct
    12541 tttgattggt gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca
    12601 agagcttgag atatctcaag ctctttttta ttttctgctc ctcaccacca ttataatctg
    12661 ctgcgaagaa ctttacgaaa gttgaataaa gaaagaaaaa tatttgacag aataaacaaa
    12721 cttgtcacaa taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg
    12781 ggacctaaaa cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact
    12841 ccttcttgag gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt
    12901 gaaaagccta aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata
    12961 ggtaaataac agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc
    13021 ttatccccta gttttcctag tttcggtgaa gaaaaaacag cggaaacacc agctatcgaa
    13081 acaatcaagc cgctgacaaa aaggatattt tctgtacttt cgctcaattc acgaatatat
    13141 aaggtcaaaa tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag
    13201 agaccgatca aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga
    13261 tcttttttct cgatcggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg
    13321 atcatcaaca aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt
    13381 gcaccgccca ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct
    13441 aacgcccatc cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga
    13501 atatatccag acaaaattcc attccaaaac cgcatgatca aaagccaata cgcgttcggt
    13561 acaaaagcca aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt
    13621 tttcgtcctt ttcgatcagc tagattcccc cagattggcg caaccacggc agatgcaaaa
    13681 gcagtcacag agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt
    13741 tgctcaacat aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa
    13801 cagccaatcc acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct
    13861 ttcgtctctt ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag
    13921 agaaaaatcc cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag
    13981 atcagaacaa atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa
    14041 gttgataatg aataaaatat caacgatcca caaagcaaca gaaacttcat tgtattttcc
    14101 tttgattact ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct
    14161 atagcacaat cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag
    14221 ccatttgatg tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc
    14281 agttgcttga tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag
    14341 cactgccact actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga
    14401 ttcgacataa gttgttgtat tcgatgtacc aaagatagcc cccacggaag tagcgatcgc
    14461 atcggcaaat agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat
    14521 ctcgtcttct tttgagaaaa tgcctgtgcg gcgtcctgta ccgataaatg ttccgatcgt
    14581 atcgaaagta tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga
    14641 agaatcactg aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc
    14701 taattcttta aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac
    14761 aacacccata aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg
    14821 gacatttttt acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg
    14881 agcgttgtca aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc
    14941 ttttccgcct tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa
    15001 tcctgcattt ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg
    15061 ttgaagactt tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa
    15121 aatattgatt aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag
    15181 cccaaaaaca accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata
    15241 tggtacattt gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc
    15301 tagaaatact gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa
    15361 gatataactc atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt
    15421 attgtttttc tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt
    15481 ctgatggaaa actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt
    15541 caaattatat agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata
    15601 atacttaacg ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt
    15661 tgttaggaat agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg
    15721 ctagttgagg aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg
    15781 aactaccctc aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc
    15841 aatcaatgcc ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca
    15901 gttttaccaa caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca
    15961 catccctaat cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt
    16021 tgaaatctta tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga
    16081 tacttttttt attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc
    16141 aggagagaag aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt
    16201 ccgaacagac aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag
    16261 ttatgtcgat gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg
    16321 aatccaaaaa gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga
    16381 cgtcattgcc tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg
    16441 agtagctatg gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt
    16501 gagcaatcag gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag
    16561 aattagattc cacaaaaaag aggttgtgac a
//
LOCUS       DACDNO010000063        16453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDNO010000063 DACDNO010000000
VERSION     DACDNO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.063"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..484
                     /gene="scm"
                     /locus_tag="IWI22_002189"
     CDS             <1..484
                     /gene="scm"
                     /locus_tag="IWI22_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9169564.1"
                     /translation="SDSTDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDS
                     STTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSD
                     NRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR
                     "
     gene            complement(639..1625)
                     /locus_tag="IWI22_002190"
     CDS             complement(639..1625)
                     /locus_tag="IWI22_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9169565.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1648..2214)
                     /locus_tag="IWI22_002191"
     CDS             complement(1648..2214)
                     /locus_tag="IWI22_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9169566.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2228..2644)
                     /locus_tag="IWI22_002192"
     CDS             complement(2228..2644)
                     /locus_tag="IWI22_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9169567.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3101..5035)
                     /locus_tag="IWI22_002193"
     CDS             complement(3101..5035)
                     /locus_tag="IWI22_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9169568.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5337..6272
                     /locus_tag="IWI22_002194"
     CDS             5337..6272
                     /locus_tag="IWI22_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9169569.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6362..7465
                     /locus_tag="IWI22_002195"
     CDS             6362..7465
                     /locus_tag="IWI22_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9169570.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7478..8431
                     /gene="menA"
                     /locus_tag="IWI22_002196"
     CDS             7478..8431
                     /gene="menA"
                     /locus_tag="IWI22_002196"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9169571.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9245..10591)
                     /gene="gor"
                     /locus_tag="IWI22_002197"
     CDS             complement(9245..10591)
                     /gene="gor"
                     /locus_tag="IWI22_002197"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9169572.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10725..11966)
                     /locus_tag="IWI22_002198"
     CDS             complement(10725..11966)
                     /locus_tag="IWI22_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9169573.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(11984..12907)
                     /locus_tag="IWI22_002199"
     CDS             complement(11984..12907)
                     /locus_tag="IWI22_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9169574.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13128..15620)
                     /locus_tag="IWI22_002200"
     CDS             complement(13128..15620)
                     /locus_tag="IWI22_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9169575.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15627..16094)
                     /locus_tag="IWI22_002201"
     CDS             complement(15627..16094)
                     /locus_tag="IWI22_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9169576.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16230..16303)
                     /locus_tag="IWI22_002202"
     tRNA            complement(16230..16303)
                     /locus_tag="IWI22_002202"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16267..16269),aa:Arg,seq:acg)
     gene            complement(16361..16434)
                     /locus_tag="IWI22_002203"
     tRNA            complement(16361..16434)
                     /locus_tag="IWI22_002203"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16398..16400),aa:Pro,seq:tgg)
BASE COUNT         4855 a         3445 c         2929 g         5224 t
ORIGIN      
        1 aagtgatagc acggacacgt cagcttcaag cgacagtacg gatacatcta ccacaacaag
       61 tgatagcacg gacacgtcag cttcaagtga cagtacagat actacctcta ccacaagtga
      121 tagctcagat tcatctacca ctacaagtga tagcacggac acgtcagctt caagtgacag
      181 tacagataca tcctctacca caagtgatag ctcagattca tcaacttcaa gcgatagtac
      241 agacacgtcg tctaccacaa gcgatacacg tcatgcaagt gtctccttct caaaaacaag
      301 ttcggataac aggacgaact ttaacttaac taataaaaca agcaatgaca atggaaacaa
      361 atcaaaaaga gcacttccta aaactggaag tcaatcgaat aattggataa cactcgcagg
      421 cgttatatta cttgtaatcg gtttaagaat cacttttatg agttataaga aatcgaaaag
      481 gtaaaaaaat attcaagata aaaaagagca acgtcgctaa cggacgttgc tctttatttt
      541 tgctttttat cataaataca agaactacaa ttcaatttgt caaaaaacac gtagatttcc
      601 ttctgagaaa tttatttacg tatcgaacct ttccctattc agtctttgcg atccaaaatc
      661 agttgggtaa gggaaaagag attctctttt gttcgaagag aggtggcagg taatttcttt
      721 atttccttca aagccttttc tgtgtaagag gccgctaact tcctagcttc ttccacgcct
      781 cctgtatgtt caacgatttt gcgtacggcg tcgacatctt cttctgtcat tgccgctttc
      841 ttctctaaat aaggtagtag ctttggtttg tttttttgca aagagtagat caacggtaaa
      901 gaatagaccc cttgacgcat gtcttctagt acaggtttgc cgatttcctg gctctctttt
      961 gtataatcta aaatatcatc gataatctga aaggccatcc cgatagaaag tccgatatct
     1021 cctgcttttt ttgcgaagcg ctcgcttgtt ccactttcaa aagcgccaac agaacaactt
     1081 aattggaaca gttcagctgt cttacctgaa atattttcca aatattcttt tacagaaagt
     1141 tcataattat agcgatcatc catctgccct aattcgccat tcaatacttt ttccatactg
     1201 cgagaattca attggagact tttcaaggaa gtggagtaat cagacaatag tttaaaacag
     1261 cagacaaata aatagtctcc tgcatagaca gaaacgacat taccaaactg actacggata
     1321 ttaggctgac cacgtcgcag atccgcttca tcaacgatat catcatggat cagtgtggcc
     1381 gtatggagca tttcgataga agctgccaaa gcgatggctt tgtctcgatc ttgctctggt
     1441 ccaaattgtg agaacaatag ctgataggca ggacgcaaca gctttccgcc ggcatggatc
     1501 gtttctttga tggcttgttc gacaggtttg tttttcaggc ggacagtttc ttccatccgt
     1561 tttagtgttt ctgtcaattc tttacttaaa gcaggatagt tttcccacat tggatgtagc
     1621 ttcataaaaa aactccttgt gtgacgatta ttttttggta aacattgggc tccatttttg
     1681 tttggttccc tgctcataat ctttttggaa tcgttgaagt gtctgcacat gtcgtaaaag
     1741 ataattcgct gcgatcccga ttgcaatccc tgataaaatc cctaaaactg aaaggactgg
     1801 caagtacaac atgactgtcc atgattgtgc aatgaaagaa gccatcaaaa gctgtccgac
     1861 attatgcatg aaaccgccgg ccgcactaat tccgatgata ctgacacgtt ttggtcctaa
     1921 ttgcttgacc aataacattc caaaataact aagcaaagca ccactcatac tataaaggaa
     1981 tgtggaaatc gttccgccta gcaaagtagt caggaatagt cgtagccaaa gtagtaagaa
     2041 gctgtcttgt tttttcatgg taaaaatggc aacgatcgtg attagattgg ctaagcctag
     2101 ttttgcacca ggcgcaaaag aaaaaggata agggatcata ttttccaata aaccaatgat
     2161 cactccttgt gcaacaagta gcgaaatata aatcattttt tgtaatttac tcatggtgtg
     2221 tgaaggtcta ataaatcaga ctgtcttcgt cacctccatc tgttgaacgg acttcgatca
     2281 ctaatttatg tggcaaacag acgatggttt cgccattttt cgttgcccag cctcgacgta
     2341 cgcagatctg gtcatcgcaa tctgcttctt ttatccgtac tcgatcgcca tctatctcga
     2401 tcaagttata atctccgtgt tcatctttgt aagtatatgt gttgctctct tctccagcca
     2461 tcaaagggaa tgttttgatt tctgtgccat ccacacgcag taccgcttct ttttctgctg
     2521 ttgcggtgtt ttgctgaaga ccaaaaatca tgactggtaa aaaagaaaga acagtcaaaa
     2581 gcaaaataat aaaaacatcc caaggtttga gcagactttt ttcagcaaat tctttaaggc
     2641 tcatttttgt tctccttttt ccagattaac tcacccaatt ttagcataaa aacaagaggt
     2701 tgtgacacct gtgtcttgct ttgagtaata agaacgaaga atagaaaaca gctttccatg
     2761 ttttctaatt ctttgggctt attaccgaga agcagcaggt ggaacaccgt ttataacaag
     2821 aggttgtgac agaagtgggc aacttcaaga aataagaagg aatttacgaa aattactttt
     2881 caagtttttg tgaaatttcg gcttattttt cgaagaagct acttctggaa caccgtttat
     2941 aacaagaggt tgtaacagag gttgtgacac ctgtgtcttg ctcatggagg cttatctcgg
     3001 gcatcttatt cctttaattt tacataaaaa ggaggctgtg acggtttgtc tcagcccctt
     3061 tctattgaaa tcttgcattc aaataacgtt ctttatctat ttatttcttg ccgtactggg
     3121 aacttggtct accgtaccac caattaccta gtgtacgttg tagccatgga attacccagc
     3181 gatccaaccc aatcgcacgt ccggaaccgt tcatcaatgc gaaagcaaca aaaatgaacc
     3241 aaatgtttac ccagtaaaac atgcctgata agcagaacgt gatcaccaat acaatcgttg
     3301 tcgcactgca tagccatgtg aataatccag caatcaatgc taatgccaag gcaatttcaa
     3361 agaataccat gaatttttgg aagaacattg ctacatcatt gtttggcatg atcacttgca
     3421 tgatctttgt gaaccagtct ggtgacttct caatcacagc cattggttct tcaccgtatg
     3481 cataactcaa accaaaatgt gcagcttgag tcgctgcttc tccggcactt gcccctgctc
     3541 cgctggcagc tgtggttgca tcaccagcag cagaagcacc actggcaaca tctgttacat
     3601 tgtattccca tgtccaaggg aacacactgt ttccagtgaa ccatgatcct tcaccaaacc
     3661 aagtacttgg tttgaacact tcaccattcc caatgatttt tttggaagct tcaacgaacc
     3721 aaactaaacc atagaaaata cgaagtggca cactccataa gacattgccg taacgagaag
     3781 aatgtcctcg agtcacgttg cgatcatctt tgatatggaa aaactcatgc attaaatatt
     3841 ggaacatata ataaccagaa cgaatatcaa agaagtattt caaattcacg atatgtttca
     3901 ttacgatagc taaaaatccg cttaaatgga tcttatcaaa taggttagct accccccatt
     3961 tggcaccaat agaaaccata aatccttgat agtttccttt aaatttatgc ttttctccgc
     4021 ctttgatcga agccacgata ttcgctgcgg ctgtgtgtcc tgtttgttca gcagcttgaa
     4081 cgatctgcgg tgtcggtgtt cctgggaatt cttcataaaa gactaaatca ccaatgatat
     4141 agatgttttt atcttcgtag ccttttgcct gcatatattc attggcgatc aaccgctgtc
     4201 cgcgtgctga ttctaatcca aagtcagcag cgtcagaagt tcctttgaca ccagctgtcc
     4261 aaatcagtgt gtgagtcgga atgttgcgtc cgtctttcaa tttgatatga tcagcagcca
     4321 cttctacgat tggtgcattc aataataatt ccacgttgtg cttacgcaaa tagcgttcag
     4381 cttttgcagc atcatttcga tccaacatat tcaagatcgt cggcattgcc tctacgacca
     4441 tcagtgtgat ttcgtttgga tctaatttat aatctttggc taggcgattt ttccagtcga
     4501 tcagttcccc aatcatttca ataccggtaa atccagaacc acagacaacg aaagtcagca
     4561 tggctttacg cagttcatca tctggttcca atgcagcttt tgctactgtc ttctcaatat
     4621 gttcccgaat ccttacagaa tcttcaaatg accaaagggt gaatccgtgt gctttgacac
     4681 caggtgtacc aaagtcattc ggctctccgc ccattccaag gatcagctga tcaaaaggat
     4741 aggaacctaa tttggtcttg acgatttttc gttctttatc gataccgatt acagtgtctg
     4801 tgacaagttg tacattcttt tgacgagcaa acaaatgctg cagatcgtat tggattgctt
     4861 ctggttctac acgtccgcca gctacttcat gcaactcggt catcatagta tggtacgaat
     4921 gacgatcgat cagagtaatt gctacatctt cttttttcag tttctttgcc aagtattttg
     4981 tagctgaaac accggcatat cctgctccta caacgacaat attttgcttt gccattgatc
     5041 atccgctcct tatagactat ctatataatg aatatatgat aattctttta gtttgatact
     5101 tattacacaa tctccatata ttatatagat gttctgttct tttgtctaac tccttttgga
     5161 tttttttcaa attttaaaat aaagcgtttt ttcacgaaat ttgctgaaag aaaacgtttg
     5221 aaatattaca tgggttattt atcaaaaata gttgacttat aggctaaaaa gattagaatg
     5281 tataatgaat cacaaagaaa aattgtggac tagaaatgga atggtggggt attaaaatga
     5341 agaaaaaacg ttttgtaact ggatttgccg tattagcatt ttcagctcta gtgttaggcg
     5401 cttgcggcgc agatgacaac ggaagctcaa actcttcttc tgaatcatct acagcacaat
     5461 cttcgactgc aaaaagcaca acagaatcat ctgcaaaagt tgtagctggc ggagatttgc
     5521 aagacggcac atataaatta gaagaaaaaa attattcaaa tggttaccgt gcagtctttg
     5581 aaatggttgt aaaagacggc aaaatcactg aatcaaaata tgataacgtc aatgaaaacg
     5641 gcgaatcaaa aacaaaagat gcagaataca acaaaaacat ggaagcaaaa tctggcacaa
     5701 acccagaaaa atacattcct gagttgaatg accaattctt aaaagcacaa agcgcaagcg
     5761 gtgtggaagt cgtaactgga gctactcact catctgaaag cttccaaaac tacgcacaac
     5821 aattgatcca agctgctcaa gctggtaaca cagatacaat cgaaatcgac aatggtgctg
     5881 atttgaaaga cggcacttac aagcttgaag aaaagaacta ctctaacggc taccgtgtgc
     5941 aattcgaaat gacagttgct ggcggcaaag taactgaatc aaactttgat tatatcgaca
     6001 aagacggtaa atcaaaacaa gatgatacag aatataatga aaatatgaaa gcaaaaagcg
     6061 gtacagaacc aaaaacttac atccctactc tgaacgacga attcgtcaaa gcaatgggcg
     6121 aagaagatgg ctcacctgct aatgtagaag ttgttacagg tgctacacac agctcacatt
     6181 cattcatcat gtacgcacaa caattagtga atgcagctga aaaaggcgac actcaaacaa
     6241 tcgaagtaga taatatcgta acagaaaaat aatcacataa tcatactaat aaaagttgga
     6301 gggtgggacg atagccagat taggcagtcg tctcaccttt caaaatgaag agaggaaaaa
     6361 gatgcggaaa aaactactat tttccttaat cacactgggc atctctttta tggtgctaac
     6421 tggctgcagc aactcagcaa ataaaacaac cactgaaagt tcagcagcta ccaagatatt
     6481 gaaagatcca tattctgatg aacagttctt gttagggact tacgttcgta tccgtgtgta
     6541 tgatgaaggc aaagaatcag cattgaaacc tgccttcgat cgagtaaaag aacttggtga
     6601 taaaatcacg atcaatcaaa aaggttctga gatcgatgaa gtgaatgagc aagctgggat
     6661 caaaccagta aaagtttctg atgacgtcta cacgttagtc aagcgggcct acgaatatag
     6721 ccaagattct caaggcggat tcgatatggc gattggtgca atcacacaat tatggcgaat
     6781 cggttttgat gacgccagaa aaccttccca agaagaaatc gatcaggcat tgaaattagt
     6841 ggattatcat aagatcgaac taaatgacaa agaaaaaact gtttatctaa aagaaaaagg
     6901 aatgatcatc gatttaggtg caatcgccaa aggttacatt acggacgaag tagtgaaagt
     6961 cttgaaaaaa caaggagtga ccactgccat cgttgacttg ggcggtaatg tctatgtctt
     7021 aggccacagc ccaagaggcg aaaaccaaga ctggacagtc ggcatccaag atccgaacat
     7081 ggctcgcgga tctgttttag gttctattaa agaacggaac aaaacattag ttacctctgg
     7141 aatttacgag cgttatttag aagtagatgg caaaaaatac catcatttgt ttgatccaaa
     7201 aacgggttat ccgttcgaca atgatatcgc aagtgtcacg atcataacag ataaatcaat
     7261 tgatggtgac gggttgtcta cagcagtctt ttcaatgggt gtgaaaaaag gactggaata
     7321 cgtcgaaagc gaattgaata acgggacgga agcgatcttc gtgacaaaag acgataaagt
     7381 atatgtgacc gatccaatca aagacacctt caaattatcg gaggactccc attacacgat
     7441 gggtgaccgt tctgaactaa aataaggagt acgaaccatg acactaaaaa tctttttaga
     7501 agtagttgaa atacgaacaa aggtggctag tgtatttcct tttgcggtag gggtattatt
     7561 ctctatcgct ttttttcatg agatacattg gctaaacact tttttatttt ttattggaat
     7621 gctagttttt gatttagcga ctaccgctat caataattac atggattatg aaaaggctca
     7681 ttctgaagta tataagtatg aagaaaatgt cattggccgc agaaatctat ctcctgtatt
     7741 gattcgaaac atgattttag ctatgattgc atttgttttt gttatcggcg ttattctaac
     7801 gatcaatacg ggttggcttt tacttttgat gggatttatc tgttgtttta ttggagtatt
     7861 ctatacattc ggaccgattc ctttatctcg aatgccttta ggggaaattt tcagtggggt
     7921 gactatgggc cttggaattt ttgccatggt cgtttatgtc aatacattcg gttcagatgt
     7981 ctatgatctg acagtcaact tttcatctgg acaatttgat ttggtaggaa atctttgggc
     8041 tatagcagca atcgtattag cttccttacc cttggtgttt acgattgcca atatcatgtt
     8101 ggctaataat ttgcgggatc ttgaccgtga tattgaaaat catcgctata cactagtttt
     8161 ttatatcgga cgtcctgcgg gaacagtctt attccagcta ttgatgtatg cttgctattt
     8221 cgtgatttta atggggttgt tgagcggtat ctatgaatgg ccgatcttgg tcaccttctt
     8281 gaccttgcca attgtgaaga agcatttgga tcaattcaag caaacgttac cccagcctat
     8341 cagtttcagc tattccatca aaaatatggt cttattcaat gccagttatg tattggggct
     8401 attactaaca atcattatgc aagccattta acagcatgaa gaggttgtga aaaaactgcc
     8461 ctgcatcaag taataagaac agccaaataa aaatagctcc ttatattttt cgtttggttg
     8521 ggcttattac cgcagatgca agcttttgaa caccgtttat caggaggttg tgaaaaagtc
     8581 gcttagtttc gagtattaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa
     8641 tttggcttaa taccgagaaa ctggcttttg aacaccgttt atcagaggtt gtaaaatcag
     8701 tgttttgtcc tgagtattag agaaacaaat ggaaaaatag ttcctcatat tttgccgttt
     8761 tttgagctaa taccgaagga cagctgattg aacactattt gttcggaaaa agtgactaac
     8821 tatgacacaa tttttttcac agtccctttt ttcacttgat ttgattcgta ccagcgaact
     8881 tgacgagaac tttcttggtt tggtaactat atggtattct tttaacatcc tcgtttgctt
     8941 cttctaattt atttatacaa agtcacactt ttttaaacat ctgttcttcg atacatttct
     9001 atttctttta aacatcgata ccaatatttt gaacttaaga gtgcatacca aatggattca
     9061 tttactcttc gtcagttacg aagataatca aaatctccaa cttccctact tatttcgttt
     9121 tgagaattat cgaagcaatc ttacataaat aactaaaaaa caaaaaacca gaaaatcagt
     9181 atactgattt tctggtttta catggtttgt tcaataaaaa gctgttggaa ctgttaatat
     9241 taaattatcg catcgttacg aattcttccg ctcctgttgg atggatggct actgtattgt
     9301 caaagtcttc tttagttgca cccatcttga tagccacggc aaaaccttgc agcatctcat
     9361 cgacaccaac accaatagca tgtaagccga cgattctttc ttcttttccg acacagatca
     9421 atttcatttc gcatttttgg cgataatcat ttaatgcaaa gtacattggg gtaaagcgtg
     9481 aacgataaac tttcaattca ttttcgccat atttttccaa tgcttcttct tctgtcaaac
     9541 caattgttgc aattggtgga tgcgtaaaga cgactgtcgg aacaagttta tagtcaagat
     9601 aagaatcttt ctttccatta aataaacgtt ctgataaacg tctaccagct gcgattgcaa
     9661 caggagtcag atctaattta ccgatcacat caccaacagc aaaaatatgt tcatccgttg
     9721 tattttggta tttatctact ttgataaacc cttttttgtc caattccaca tttgtatttt
     9781 ccagacctaa tttttcggta ttcggtacac gtccaccggc aaaaagtaca caatcaccag
     9841 ttaaggtttc accattttca aatagcaccg tatattcgtc attgttttta gtaatttctt
     9901 tagcaatatg atgtgcgtaa gtttttattc cagcttcttg gtacatttgg atcaaattgt
     9961 ccgaaagcat tttatcaaac gttctaagtg gtcgttcttt tcgatatgcc cacatgactt
    10021 cactacctaa accacgaaat acacctgaaa gttcagccgc aatgtatcca gcacctaaaa
    10081 cgattgctct cttaggtaac tctgttaatt caaaaaatcc atttgaatca attgcgtatt
    10141 ctccaccagg aatggtcatt ttacttgcac ggcctccagt agcgatcaag attttaggag
    10201 cacgataggt ttcaccgttt acttcgatcg tttgactatc gataaaagta gcgtaacctt
    10261 ttatagcctc gacatgattg ctatctaatc ctctttgata agcaccgtgt agaaaatcaa
    10321 tatatttttc tcgtctttct actagaccgg cgaaatcaac attttttaaa tctgcttgga
    10381 taccgtaact tgaggcatct cgttcaattg tttccaaaat cgtacttgct tgccacataa
    10441 cttttttagg tacacaaccg acgttgacac atgttccgcc caattcattc gcttcaatca
    10501 gcaaaacttt tgcgccatgc atccctgctc tgtttgctga agcaataccg ccagagccgc
    10561 caccaattac gatgtaatca tatatttgca ttttgttcct ccttattgaa gagatcaatc
    10621 aaaaccttta tttaacgatc ctttttctac agaaaacaag tgtaacactt ttcttgaatt
    10681 ttgtatgcga agatgcttaa aacagggata tatcagcttt ttccttattt acgttttcgg
    10741 atcatatctc caggtttcac cggctgtgcg actggcatag ttaaaatcat cattggattt
    10801 ggtgctcggt caatggattc gccttcttca ttatgcataa cttcaacggt ttgactgaaa
    10861 tgactgaatc ctggaccata aaattcgatt tcatctccta cagaaaacaa gtttctctga
    10921 cgtatcgtag caatctgtgt tgcctcatca taagataata cttcaccgac aaatttatat
    10981 actgggattt tacgacgttc accgaataat tgttcgtttt cagttggtgt attgtagtaa
    11041 aaacctgtag ctaattctcg ttgtgctact ttccataact cgtcgatcca ttcttgcttg
    11101 cagacatagt tttcaggatc ttccatataa ctgtctacag ccgctttata gacatttgac
    11161 accgtagata cataatggat cgatttcatt cttccttcga tcttgaagct gtctacgccg
    11221 ttttcaatca attctggaat atgttcgatc attgacatat ccactgcact catcgagaat
    11281 tcttcttcca cttcgcctgt atcagttaaa ctgtttcgtt cttgaccaaa aggcatatcg
    11341 aacaatccat acttccagcg acaagattga gaacatccgc cacggtttgc atcccgcatc
    11401 gacatatggt tggacaatgt gcatcgacca gaataagaga tacacatggc accatggata
    11461 aatgcttcga tttcggtatc cgtattttta cgaatctctg ccacttcctc cattgaaact
    11521 tcacgagcta aaacgacacg ttccaaccct tcttctttcc agaattccaa tgtctcaaaa
    11581 ttagttgctg aagcttgagt ggataaatgg atcggcagac ctggggcttc tgtcgcacaa
    11641 atttcgatca aagctggatc agaaacaatc actgcagaaa taccgacatc acgcaaactg
    11701 cggaaaaatt cccctgctcc atcttcattt ccttcatggg tgaccatatt agcagcaacg
    11761 tataccttcg catcatgttc ttttgcaaaa gcaactcctt cagccatttc ttcataagta
    11821 aaattacctg cacggcttct taaaccatat gcattcccgc caatatatac tgcatctgct
    11881 ccataatgga tcgcggtttt taacttttcc agtgttcctg ctggcgctag gatttctggt
    11941 cgtttcaatt ttctttttac tgtcatttct ttcatcatac tccttatttg atagcagaag
    12001 gatcaatata aaagaatcct gtatctaatt cacggttttc aggatgcaat ttactgattt
    12061 cgctagtcat tttttccgct ttttcttgat cccagctacc ttctaaaatt tgtttttttg
    12121 cttcggcgaa tagtcgagtg atgtttacga atttttcgcc tggagtaaat aatccgtcca
    12181 atttccatgt acagtaatga tgctgataaa gttcattcaa ttcattcgag agattcagat
    12241 cattgctggc aaaaatatgt gtgccatgac tatcttcata aatagaataa tgtgtctctt
    12301 cttttttagg ttccgagata aaaagacctc tttcacgatc tttttgttcg tcttgtttcg
    12361 tatagttata gtaattctgt agcagtggac gtttggattg atggatgcat gtagcaccat
    12421 acacaagggt ttctacagga atatcgagtt tttcttccat cgccttcatc tcttcaaaag
    12481 gaacttcacg agcaagtact gctccaactg cgcctttttt cgcccagaaa ttgatttgac
    12541 gtgcgctcgt tactaatgtt tcagcatcat aaatatacgg tatacgcaat tcaggatttt
    12601 ttttcatcgt ataaataata cctgtatcac caactgtgat cacatctacg tttatttctt
    12661 ttaaaaaagc caaatattca ggtacatttt tcattttttc aggatgaatg atcccattta
    12721 cagcaatatt cgctttttta ccatacttgt gtgctaattc taccaattgt cgctgttctt
    12781 ctcgtgtaaa tgaagcaggc agacgcaatc caaattgctc ttcaccgaaa tatagtgtat
    12841 ctgcacctgc agcaagcaac tgttctgctt gttcaatagt ttctactgtg gttatgagtt
    12901 cgatcataat tttctcctcc tacaacttca taatagtagc atagctctaa aaactttcca
    12961 atacttcttc tatttaaagt cggatttttg aaaattattt cacaaaaaat caaaataaag
    13021 ttcgatatat tctgtccaaa tataaaaaga gattgtgaca aaaatcttgt cacaacctct
    13081 taatcaagtc ttactattat aaaaattcag ctacacaggc caagtaacta tagggaacct
    13141 actgtttcct ttttaggtcc gcgaacattc aatgtgatct taccttttga agcacctact
    13201 gttacgtggt cacctaaacg aatttctccg ccaagcaaag cctcgctcaa tcgatcttca
    13261 atttcttttt gaagtgcgcg acggattgga cgtgcaccat attcaggatc aaatccagct
    13321 ttcccgatca catcgattgc agatggtgtg attttcactt gaatatcttg ttctttcaat
    13381 cgtttgataa tcgctttgct catgatttgg acgattgtat ggatttcatc tttactcaat
    13441 gaatggaaga cgacagtctc atcgattcga ttcaagaatt caggtcggaa agctttcttt
    13501 aactcttcta agatacgttt ttgcatggct ttgtgatctt tagtaacatc ttggacattg
    13561 aatccgacat ttttttcttc tcgaatttgt gtcgctccga tatttgaagt cataatcatg
    13621 attgtatttc taaagtccac ttttctacct ttagaatctg ttaaatgacc atcatccaat
    13681 acttgcagaa gtaaattgaa aacgtctgga tgtgctttct ccacttcatc taagagaatc
    13741 actgaatatg gtttttgacg aattttttct gtcaactgtc caccttcatc ataccctacg
    13801 tatcctggag gagacccgat taatcggctt gtactgtatt tttccataaa ttctgacata
    13861 tcaacacgga tcagggcatc ttcacttcca aacatgactt ctgaaagtgc tttggccaat
    13921 tctgtttttc cgacaccagt tggtccaagg aacatgaatg aaccaattgg acgatcagga
    13981 tctttcaatc cgcttcgtgc acggcggatc gcacgtgaaa ctgctttgac agcctcatct
    14041 tgaccaacga cacgttcatg cagaagtcct tccagttcta acaagcgttc gctttctttt
    14101 ttctccaact gctgtagagg gacaccagtc cactccgata caactgtagc aacatcttct
    14161 tctgttacac gatcagaata accttgctcc tgttttgctt caattgcaga aactttttgc
    14221 agctttttac gtagtgcttt ttcttgtctg cgtaattgag cagcattttc aaagtcttgt
    14281 ttttggatcg ctgcttcctt ttcttgtact agttcatcga tttctagttt gatcacagtt
    14341 gattttgtta agtgatcggt ttgatccaaa cggactttcg ctgctgattc atcgatcaaa
    14401 tcaattgctt tatcgggtag ttgacgagaa gtgatataac gaacagaaag attgactgct
    14461 gctcgtacag cttcatctgt gatctccact ccatggtgct cttcatatct tgtacgtaat
    14521 ccttgaagaa tcacttctgc ttcttcagga gttggctcat ctacttgaat gcgagcaaaa
    14581 cgtcgctcta atgcagaatc tttttcgata tatttttgat attcatctag ggtcgttgct
    14641 ccgatcgttt gtaattctcc tcgagctaac gccggtttca agatattcga agcatcaatt
    14701 gctccttctg ctccacctgc accaattaag gtatgcaatt catcaatgaa taagattact
    14761 tgaccatcat tatagatttc gtcgatgacc tttttcattc gatcttcaaa ttcaccgcga
    14821 tatttcgtac cagcaaccaa agatcccata tcaagcatca tcaggcgctt ttcttgcatg
    14881 tcttctggca cttcacctaa aatgattttt tgagctaacc cttcagcaat ggctgttttg
    14941 cctacaccag gttcacccac cagtacaggg ttattctttg tccgtcggct aagaatctgg
    15001 ataagccgtt tcacttctct cgaacgacca acaactgggt ccagcttgtt ttcttttgct
    15061 aatttcgtca aatcacgtgc taatgaatct agcgtaggtg ttccttgcgg tgtttgccgt
    15121 ttatcttgta catttacatt tctacgacga ttcattcctt ttccagcaga atcaccagct
    15181 agccccattt tttgtttcag aacttgacgg gcttttgaaa ggctgatacc taagttgatc
    15241 atgattcgag tagccatgat actttcatct tttaataagc tcatcaaaat atgttctgtt
    15301 ccaacttttt gagaaccagt tttcttcact tcgctgctag ctaaagcaaa aacttgtttc
    15361 attcttggtg aatatggaag aaagactcct ttagggtaag aacggatacc accataacca
    15421 atcagatgtt caatttcttc ataaatttcc gtttcattca aatccatttc acgtaatact
    15481 tttccagcaa ttccattttg ctcgaccact aaggctagta aaacatgttc tgaactaact
    15541 gattgatgtt tcaaccgttt tgcttcttct tgtgcaattt ctaatactgc ttgggctctt
    15601 tcagtaaaaa gttcttccat ctaatctcac ttcccatctt cataacttaa acgttccaga
    15661 aatgctcgga gaatccgagc acgtgtatga tcttcatatt cattgaaatt caatgtgttt
    15721 tttgcaattg cactcaacat caagtttcct tctttttttg taattatttg gtcttcataa
    15781 agtttttgaa taatagcaaa agcatttttc tcactgattg tatcatcaaa taactgattt
    15841 atctgctcaa gtaattgttt tttatctgaa atccttactt ttgcaattcg tatataacca
    15901 ccaccaccac gtttgctttc tactagataa ccacgttgga tggtaaaacg ggtattaatc
    15961 acatagttga tttgtgaagg aacacagtta aagaggtctg ccatttcaat ccttcgaatc
    16021 tcgatcattt cactattttc cagaattttc tttatatatg attcaattaa atccgaggta
    16081 ttctgatgtg ccatcttatt cattcccttt gactaatatt gaccttaatt ataacgaatt
    16141 tgataataga aagaaagaag aacgcttttt gttaaataaa tcctttagaa tcaacaaaaa
    16201 aaaatacgga atatactatt ccgtatcgac gcgcccaaga ggagtcgaac ccctaacctt
    16261 ttgatccgta gtcaaacact ctatccagtt gagctatggg cgcatattca atttaaaaaa
    16321 attataaatc atttgaaaac aaaaagcaag ataaaaaaaa tcgggaagac aggattcgaa
    16381 cctgcgaccc cttggtccca aaccaagtgc tctaccaagc tgagctactt cccgtttaaa
    16441 aaataaaaaa gac
//
LOCUS       DACDNO010000064        15498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDNO010000064 DACDNO010000000
VERSION     DACDNO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.064"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1044
                     /locus_tag="IWI22_002204"
     CDS             <1..1044
                     /locus_tag="IWI22_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HAP9169577.1"
                     /translation="LEQAVKAGFVTEDNLYIDSTHIKANANKHKFTKEMTYGQAKAYQ
                     DELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGEREKQFAYSAHTS
                     CEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGYKTPKFVHFLSHL
                     KLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTITRDGYRTYKSNPK
                     FCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIYKKRKQTIERIFA
                     DAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPMAFYLKFLQRLTK
                     RSWQLSHSFLSTV"
     gene            complement(1047..1253)
                     /locus_tag="IWI22_002205"
                     /pseudo
     CDS             complement(1047..1253)
                     /locus_tag="IWI22_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1241..1870)
                     /locus_tag="IWI22_002206"
     CDS             complement(1241..1870)
                     /locus_tag="IWI22_002206"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9169578.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(1877..2593)
                     /gene="pyrF"
                     /locus_tag="IWI22_002207"
     CDS             complement(1877..2593)
                     /gene="pyrF"
                     /locus_tag="IWI22_002207"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9169579.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKEIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(2590..3522)
                     /locus_tag="IWI22_002208"
     CDS             complement(2590..3522)
                     /locus_tag="IWI22_002208"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9169580.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDPDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(3519..4295)
                     /locus_tag="IWI22_002209"
     CDS             complement(3519..4295)
                     /locus_tag="IWI22_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9169581.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(4349..7534)
                     /gene="carB"
                     /locus_tag="IWI22_002210"
     CDS             complement(4349..7534)
                     /gene="carB"
                     /locus_tag="IWI22_002210"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9169582.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTYHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(7539..8615)
                     /gene="carA"
                     /locus_tag="IWI22_002211"
     CDS             complement(7539..8615)
                     /gene="carA"
                     /locus_tag="IWI22_002211"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9169583.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(8620..9900)
                     /locus_tag="IWI22_002212"
     CDS             complement(8620..9900)
                     /locus_tag="IWI22_002212"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9169584.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(9919..10845)
                     /locus_tag="IWI22_002213"
     CDS             complement(9919..10845)
                     /locus_tag="IWI22_002213"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9169585.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(10858..12141)
                     /locus_tag="IWI22_002214"
     CDS             complement(10858..12141)
                     /locus_tag="IWI22_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9169586.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(12155..12694)
                     /gene="pyrR"
                     /locus_tag="IWI22_002215"
     CDS             complement(12155..12694)
                     /gene="pyrR"
                     /locus_tag="IWI22_002215"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9169587.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(12929..13840)
                     /locus_tag="IWI22_002216"
     CDS             complement(12929..13840)
                     /locus_tag="IWI22_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9169588.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKPAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(13837..14301)
                     /locus_tag="IWI22_002217"
     CDS             complement(13837..14301)
                     /locus_tag="IWI22_002217"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9169589.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLVLAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICILIYIILDEKDQRKK"
     gene            complement(14306..15004)
                     /locus_tag="IWI22_002218"
     CDS             complement(14306..15004)
                     /locus_tag="IWI22_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9169590.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(14991..15377)
                     /locus_tag="IWI22_002219"
     CDS             complement(14991..15377)
                     /locus_tag="IWI22_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169591.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINDEKRSVLSHEKKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
BASE COUNT         4287 a         3501 c         2771 g         4939 t
ORIGIN      
        1 ctagaacaag ctgtaaaagc tggcttcgtt acggaagaca acctttatat tgattctact
       61 catattaagg caaatgccaa taaacataag ttcacaaaag aaatgaccta cggtcaagct
      121 aaagcttatc aagatgaatt agaagatgaa ataaataaag aacgtattgc tgtaggaaaa
      181 cgtcctttta catgggacac agaaagtgag ttgagtcttc aaaaaataag tcattcagat
      241 ccagaaagtg gttactatgt taaaggtgaa cgagaaaaac aatttgctta ttccgcgcat
      301 acttcctgtg aagataatgg ttttatctta tctacattaa tcacaccagg aaatgtacat
      361 gacagccaag tcgcaatcga tctggtcaaa cagtcgaaaa gagcttttcc aaatataaag
      421 cgtgttgtcg ctgatgcagg atataagacg cctaaatttg ttcattttct gtcacacttg
      481 aaactctacc cgattcttcc atatactgta cctcatggag taaaaggaat gtttcgtaaa
      541 aaagactttg tttatgatgc ctattttgac tgctatttat gtcccaataa tcaaccgttg
      601 ttattttcaa ctatcactcg tgatggttat cgaacctata aatcaaatcc aaaattctgt
      661 aggagttgtc cattacttaa aaactgtact ttgagtacaa aacatcaaaa aatagtaact
      721 cgacatgttt gggcggatct tatggatgaa cttgaacacc aacgccatac ggcgttgaat
      781 agagaaattt ataaaaagag aaaacaaacc attgaacgaa tatttgcaga tgcaaaagaa
      841 aagcatggta tgcgttggac gaaatatcga ggtctcgaaa aagtcgccac gcacacgatg
      901 cttacgtttg ctgccatgaa tttaaaaaaa ctagccacat ggctatggaa agcaaaaaag
      961 cctatggctt tttatttaaa atttctacaa agactaacaa aaaggagttg gcaattaagc
     1021 cactcctttt tgtctacagt ctgagaagag attattctct tctttctgct aaccagtctt
     1081 agaaaaatga attttaaaaa aatcaaaaac tgatcacgat agaagtttct tttcatacct
     1141 gatgaaaaga tgcttctcgc aatcagtttt tggatttttg ctattccaat tgatgattct
     1201 ggagacacaa atcggatttc tctatcttca tcaagcgcgc ttaaatctgc caattttcag
     1261 gatctttttt ccattctttt agcagagtta attcttgctc atcgatataa cgatcttcta
     1321 aagctgcttc aatcaataca gaataattgg tcaacgtcat aagagggatt tcggcttttt
     1381 cgaaatttgc ttttcctttt ggcaactcat acgtaaagat agccgcaaca ccaaggatat
     1441 ccgcaccttc tctttttgct gcttcagctg cttccaagac acttccgcct gtagaaatca
     1501 aatcctcgat cacaaccatc ttctgacctt caaatatacg tccttcgatt tggttacctt
     1561 tcccatgatc tttcgcctta cttcgaatat agaccattgg caaatccagt atttccgcta
     1621 cccatgcggc atgagggatt cctgctgttg cagttccagc aatcacttgt acttcagggt
     1681 aagcttcttt gattttgctt gctaatcctt ttgcgatctc ttttcggact tttggatagc
     1741 tcatcgtgat tcggttatca cagtaaattg ggcttttgat tccacttgcc catgtaaaag
     1801 gattacttgg atttaaaaat actgcttcaa tttctaataa gtcttttgcg atactttgtt
     1861 caacattcat tttaggttag ctccattctg tttgaatcgt ttgatatgct ttataaggat
     1921 tagttgactg agtgattggt cgacctataa cgataaaagt tgagccgatc tcttttgcct
     1981 gagaaggtgt gactactctt gtctgatctc ctgcgactgt tccttctggt cttatacccg
     2041 gagttaagca aacaaattcg tctcctgcca tttctttgat cgcttctact tcccaagcag
     2101 aagaaaccac accatctaat cctgcttttt tggcacagct agcataatgg atcacacttt
     2161 tttctaaaga aacttcgatt agttgatcgg catgcatttc ttcttcactt gtagaagtaa
     2221 gctgagtgat agctagaagt agcggacgtt ttccaccttc tggagtcccc tcttctaatc
     2281 ctcgcattgc cgcctccatc atacgaatac cgccagcggc atgaacacag gtgagatcaa
     2341 caccaagctt tgccaatccc cgcattgctt tttcgaccgt attcggaata tcgtgaagtt
     2401 tcagatctaa aaatacatta tgaccagcct cttttagata acgtacgatt tcaggtcctt
     2461 cttggtagaa caattccatt ccgattttca caaacaattt ttcttctttc gggaaaaatc
     2521 gaagaaagtc ttctacctct tgcctagttg aaaaatctaa tgcaataatt ggtcgttggt
     2581 ctagttgatt catcgttcac gttcctctct cacttctttt attaatcttt ccagcgattc
     2641 gatgcctagt tcgctcattc gcacagggag ctctttaatt agtttaggac agatatacgg
     2701 atcggtgaaa ttcgcggtcc ctatagctac agcacttgct ccagccataa acatctcaag
     2761 aacatcatcc actgtctgca cgccgcccat cccaatgatt ggaaggctgg atatacttgc
     2821 tacttggtga atcaaacgaa tagcgacagg cttgattgct ggtccggaga gtccacctgt
     2881 ttgattggct aaaatcggtt ttcttgtctt aaggtcaatc cgcataccaa gaagagtatt
     2941 gatcatcgta aacccatctg cccctccagc ttcgattgcc tgagcgattg gaacgatgtc
     3001 tgttacattt ggggacaatt tgacatagac aggaacagaa gaaactttct ttactgcttg
     3061 tgttaattga aaagcaacat caggatctgt tccaaaagca attccgccat gtttgacatt
     3121 tgggcaagaa atattcagct cgattgcttt cacatttggc gcttcgccaa ttttgctaca
     3181 aacttctaca tagtcttctt cacaagcacc agcaacattt gcgatgatgg gtaactcatt
     3241 gtactcctct aattctggaa gaagtttatg cataacaacg tctagaccag gattttgcaa
     3301 accaattgca ttcaacatgc cagatggtgt ttccgccact cttggtgtgg gattgccaaa
     3361 gcgggcattt ggagtcgttg ccttgaccat gatcgatcca agctgattca aatcatagta
     3421 ttttccgtat tctttgccaa aaccgaaaca gccgcttgcc ggcatgatag gattttttaa
     3481 ttctagaccg gggagcttga ctgctaaagg attctcattc atataattac ctctcctatt
     3541 ttaaaaatcg gtccttcgtc acaaactttc acactttttg ttcctgtttc atcttccggc
     3601 gtatgacaaa cgcaagcata gcaagcccct atcccacatg ccatgcggga ttctaatgat
     3661 aactggacat tttgatgcga agaaaacagc tgttccactg tcttcaataa cccgtttgaa
     3721 ccacatgcaa aaacagctgt aggttccgtc ttcgttccta acaataaatt accgacattt
     3781 ccgtgcatac cgtatgttcc atcgtcagta gcgattcttg tttctcctag acgattaaat
     3841 tctttttcat aataaacgac ttcatgtgtg gcaaaaccta aaaaatgcgt tggcttaatt
     3901 cctttttcga ctaactgttt tgataactca tatagaggag ggacaccgat tcctccaccg
     3961 attataaata cttcgtctcc tgtatgaaga ttggacaaat caaaaccatt gcctaaagga
     4021 cccatgacat ctaaatggtc tccggcagaa agagtagaga agactctagt cccctcacct
     4081 tccacccgat aaatgatacg gcaagtattt gtttcgtggt cgatatgatt caagctaatc
     4141 ggtcggcgta atagcaagtc tgcacgcggc acgagaatat gcaagaactg tcctggttgc
     4201 tgcatttctt ttactagatc acctttcaat gtcatttcaa aaatctttgg tgccagctgt
     4261 ttttgctgaa cgatttccat catttcttgt ttcatctgtc ccctcctgca acggtttttc
     4321 gtctttttgt cttaacatgt gctcgccgtt aaatagggtt agttgaaaaa gcacgggatt
     4381 ccatcactcg aacgatcgca tctgctgtgt ctaaagaagt aaacaatgga ataccatgtt
     4441 caactgcttc tcttcggatg atgaaaccgt ctttagaagc atttccccga tctttatcaa
     4501 tcgtattgac aaccacttgt gttttaccat ctcttattaa gtcgactaca tttttttctg
     4561 ctgcttcctc agaaattttc gctactgttt caacgaccag accatttttt tcgaaataag
     4621 cagctgtatg tttcgttcct atcagactat agccaatttc tgtaaaacgt ttagctaatt
     4681 ctagtgcttc ttcttttgtt tcgtccgcaa tcgtaaataa tacggctcca tattcaggca
     4741 agtgtaatcc agaagcttcg aatgccttgt acaaagcttt gtctaattgt ctgtcagatc
     4801 ccattacttc tccagtcgat ttcatttcag gccctaagta agtatcaact ttctgcaatt
     4861 tcgtaaaaga aaagacaggt gctttaacat gaacttcttt tgtttctgga taaagtccgt
     4921 tttcataacc aagtgtcttt agtttctctc ccaaaatagc tttcgtcgca atttgtgcca
     4981 ttggaattcc tgtcacctta cttaagaatg gaacagtccg acttgctcga ggattgactt
     5041 cgatcacata aacctcattt tcatggatga caaactggat attcatcatc ccgacacaat
     5101 tcaaacctaa tgctaagcgg atcgtgtaat cttcgatcgt tttcttgatc gattcagata
     5161 atgtttgagg tggatacaca gccattgaat cccctgaatg gacaccagca cgctcaatat
     5221 gctccatgat ccctgggatg agtactgttt caccgtcaca aatcgcatcc acttcgcatt
     5281 caccaccaag caagtaacgg tccactagta ctggatgttc tggagatgct ttcacagcat
     5341 gttccatgta atcttctaaa tctcgctggt tttcaacgat ttccatcgca cgtccgccta
     5401 atacatagct tggacgaaca agaactggat aaccaattct cgaagcgata accacggctt
     5461 cttctttact tgtagcagta tcccctaaag gttgaggaat atccaactct ttcagcgctt
     5521 gctcgaataa atcacgattt tccgcacgat ccaagtcttc gatcgttgtc cctaagattt
     5581 ttacaccagc agcagtcaaa ggttcggcta agttgattgc tgtctgacca ccaaattgaa
     5641 cgattactcc tatcggctgt tctagctcga tcacattcat cacatcttcg aaggtcaatg
     5701 gctcaaaata taatttatcc gaaatagaaa agtcagtcga aactgtttca ggattgctgt
     5761 tcatgatgat tgcttcgtat cctgcctgct ggattgcttt gacagaatgc acagtcgcat
     5821 agtcaaattc aaccccttgt ccgatacgga taggaccaga tcccagtact agaattgagg
     5881 gcttttctga tcgtgaactt tcattttcat attcatagga actataaaaa taaggtgttt
     5941 ttgattcgaa ttccgctgca caagtgtcaa ccatcttata tacaggaatc aaatcatttt
     6001 cttttctcat ctggcgaacg gcttcttcgt ttatttccca cagctccgcc acttttttgt
     6061 ctgtaaaacc gttttgtttc gctgttttca atatcttgtc atccaacggc tgctgtttca
     6121 actgctgttc gatttctacg atatgcaaca gtttatctaa aaagaataaa tcgatttttg
     6181 tcaaatccgc taattcttct atggtgtacc ctcttctaat agcttcagct acataaaaca
     6241 ggcgatcatc ttgtgctcga accacttttt ggattaggtc atcgtccgtg atgtctgcca
     6301 actcaggaag ttcgatatgg tatgtaccga tttctaaaga acgcacagct ttcaataaag
     6361 actcttcgat attccgaccg atagccatca cttcaccagt tgctttcatc tgcgtgccta
     6421 aaagccgttc tccgttttca aacttatcaa acggccagcg aggaatttta gctacaacat
     6481 aatctaatgc aggttcaaat tctgcaaaag tcgttcctgt aacaggattt ttcatttcgt
     6541 ctaatgttaa tccaagcgct attttagcag ctagcttagc gatcggatag ccagtcgcct
     6601 tacttgctaa cgcagatgaa cgagaaacac gcggattgac ttcaatcaca taataattaa
     6661 aactatgtgg atcaagagcg agttggacgt tacagcctcc ctcgatctta agtgctcgaa
     6721 taatcgaaag agaagcatca cgaagcattt gatattcatg gtcagacaat gtttgactag
     6781 gggcaaatac gatagaatca cctgtatgga tacccaccgg gtcaaaattt tccatattgc
     6841 agacaacgat tgcattatca gccgaatctc tcatcacttc atattcaatt tctttaaaac
     6901 ctgcaatact tcgttcaatc aaacattggg tagcggggga aagacttaat ccattttctg
     6961 atacgatacg taattcttct tcattttcac acatacctcc accagttccg cctaaagtaa
     7021 aggctggacg aacgatgaca ggatagccga ttttatttgc aaaatctaca gcttcttcta
     7081 ctgtattgac gatttcagac tcggggattg gctgattcaa ttctttcatc aactgtttga
     7141 atagatcacg atcttctgct tgatcgatcg cagaaagctt tgttcctaga agttctatgt
     7201 tcaattcttc taaaatacct gatgctgcca gctccattgc catattgagg cctgtctgtc
     7261 cgcctaaagt tggaaggaga gcatctggac gctcttttcg aagaatcctt gatacaaatt
     7321 caagtgtgat cggttcgata tatacttgat ctgccacttc tttatccgtc atgatcgtcg
     7381 caggattaga atttactaaa accacttcat atccttcttc ttttaaggca aggcaggctt
     7441 gtgttcctgc gtagtcaaat tctgcggctt gaccaatgat gatcggacca gatccaatca
     7501 ccataatttt cttgatatct gtacgtttcg gcatctgttt actgctcctt tcctgcgtcc
     7561 atcatttcca taaattcatc aaataaatgt agtgcatcat gcggaccagg ggcagcatct
     7621 ggatgaaact gcacactgaa agcgggataa tctctgtgtt tgactccttc tactgttccg
     7681 tcatttactt ctacatgagt tacgatgagt ttttctggat caatcgtggc ttcatcgact
     7741 gcatagccat gattttgaga agtaaaatca attcttccgg tcgctacttc tcgtaccggg
     7801 tgatttaatc ctcgatgtcc gaatttcatt ttataggtat ctgcaccgtt ggctaaagca
     7861 aataactggt gaccgagaca aataccaaaa atcggtactt ttccttggat ttttttgatc
     7921 atttcgattg cttctggcac atcttttgga tcaccaggtc cattcgtcag catgactccg
     7981 tctggtgata gcgctaaaat cgtctcggca tctgtgttat agggaagtac tgtgaggtta
     8041 cagtttcttt tactcaattc tcgtaaaata ctatgtttca agccgaagtc aatgacaatg
     8101 actttccgac ctgttcctgg acttggataa ggtttgttag ttgagacttg ctgcacttga
     8161 ttggttgcta gaacggttgc tttcagctga tcaaatgcat gttcgaagga atctccagca
     8221 tcaacgatac tagctttcat cgttccgact tcacggattt tgcgagtaag ggcgcgcgta
     8281 tcgatacctg atattccagg aattcctttt tgtttcaaaa atgaatctag cgtcatttgg
     8341 tttcgccagt ttgaagctac acgtgcatgt tcttttacta ctactccttt gcatgttgga
     8401 gaaatcgatt cataatcatc tctatttacc ccataattac ccaccatagg ataggtaaat
     8461 gtaatgattt gaccgttaaa actttgatca gtgatagttt cttgataacc agtcatactt
     8521 gttgtaaaaa cgatttctcc gaagacacta gcatctgcac caaaagcctt tccttcgaaa
     8581 actgttccat cttctaatat aagcaaacgt tccatacttt tattctccct tctgccacac
     8641 aagttttccg ttgacaaaag tcatttgtgt ttcgcctttt actttccagc cgataaaagg
     8701 tgtattctcc ccttttgata aaaagtcttc tttatcgatc gtgcaagttt gtgtaatatc
     8761 aaaaactgct acatcagcgg gggcaccaac cgttagcgtt cctgcattca aaccaaaaat
     8821 ctcagccggt ttaactgcca accaattgat cacttgttcc aacgtgaatc gaccagtttc
     8881 tacaaaatga gtatagatca gttgaaacgc tgtttcgctt cctacgatac caaatggtga
     8941 tttcatgaaa ctttggcttt tttcttctaa tccatgggga gcatggtctg tagcgatgca
     9001 atcgatcgta ccgtcaagca agccttcgat caaagcttct ctgtcctccc gtcctcttag
     9061 cggcgggttc attttccaaa agccgaaatc ttcgggaata tcttcgtcga tcaaaataag
     9121 atgatggggt gaaacttctg ctgttacgtg gattcctgct ttttttgcgt ctctgattac
     9181 acggacactt tctttagtag aaacatggca aacatggtaa tgaacaccag ttgcttcagc
     9241 tagcaacaga tctctagcaa tttgagaaga ctcggttacg cttaagatcc cgggaagacc
     9301 aagttcttct gccttcttgc cagcgtgcat aacgccgcca aataataaag attcatcttc
     9361 tgtatgggct actagggctt tattgttttt tgcagcttct ttcatagcca gatacatcgt
     9421 cccagctgtt tggacaccta caccgtcatt tgtgaaagca aaagcacctt cttcaatcag
     9481 tgcttcttga tcaactaatt tctcacttcg cagattttct gtgattggcg catactgcaa
     9541 taccttcaca actgcatctt ttctaatgat gtcatatact tgtctcagct tttctgccgt
     9601 atcaggtaca ggatttaaat tcggcatcgc gcatacagta gtgaaaccgc cacgagcagc
     9661 tgagcgagtc ccagcttcga tcgtctcctt gtatgtaaat cctggctctc tcaaatgaac
     9721 atggacgtcc actaaaccag gtgtgatcaa ttgtcccttt gcatcaaaca cttcatcaaa
     9781 ttcggcttct gagaatcctg taccgattgc tttgattcgc ccatcttcta tccaaatttc
     9841 tgctggtgtc gtcatatttt ttcgtgtatt gatctgaccg tttttaatga gtgttttcat
     9901 tttttcatcc tcctataatt acgcttttcc atttagcact gcttctaaga ttgccattct
     9961 gacgaatact ccattgctca tttgctgaac gatccgcgac tgtaatcctt ccactaatga
    10021 atcagctaat tctacatcgc ggttaactgg tgctgggtgc ataatgattg catgtttttg
    10081 caacttcttc gcacgctcaa ctgttaaccc atgttcctga tggtagcctt cttttgagaa
    10141 actttctttt ccatcatggc gttcgtgttg cacccgaagc atcatcatga catcaacttg
    10201 atctagtagt tcatccaatg gcatataatg tccgtacact tcgaattcat cgtcgtacca
    10261 ttctctagga cctgagaaga atacttgtgc gcctagacgt tttaacatct gcatattgga
    10321 tttagctacg cgagagtgag tgatatcacc tacgatggca actttcagac cttcaaaatg
    10381 accaaattct tcataaatgg tcatcaaatc aagcaagcat tgggttggat gctgtccgct
    10441 tccgtctcct ccattgataa tcgaacactg gattgtttta ctttgtatca gttcgtcata
    10501 atagttctca tcaccatgac ggataaccgc tacgtctacg cctaatgctg acatcgtcag
    10561 tactgtatcg tatagtgttt cccctttttg gacagagctt gtgcttgctt caaattcaat
    10621 cacttctagc cctagttttt tctccgctac atcgaagctt ttatgtgtgc gagtactatt
    10681 ttcgaaaaag aggtttgtag caaagtactg gcgcttttgc ggagtccaat tagcaccttg
    10741 cttgaattcc tgccctctgc ggatcaagcc cataacttct tgatcagtaa gtgcctcaac
    10801 ggtcaataaa tgctttaagc tgatacgttc tgatttgatg atcataatgt ttcctcctta
    10861 gttttcttcg cttcgtgaag tttctggtaa gataagattt aaaatgatgc caagtacagt
    10921 tgctaatgcc attgccgaca atgtaaatgt gcctacttca aagacgagtc cgccaattcc
    10981 tactactaag ataactgaag caatcaacag atttttcttt ttatcaaaat cgattttgtt
    11041 ctcgattaaa atcttcattc cacttgccgc aattactcca aataagataa aactgattcc
    11101 tgagatgact ggtccaggga tacttaaaat caaagcgctt aattttccta cgaatcctaa
    11161 aatgactgca aatactgcag ctccaccgat cacaaacacg ctatggacac gtgtgatagc
    11221 cagaacacca atgttttcac cgtaacttgt tacaggagga cctccgacaa aggctgccac
    11281 gatttgtgct aacccatcac ctgtcaacgt cttatgaaga cccggttctt caaagaaatt
    11341 acgtttagtt agtttattca ataccataag atggccgata tgctctgtca tcgtcacaaa
    11401 agcgatgggt gccattgttg tgattgctcc aagatatagc tttggttcat attgtacaaa
    11461 gggaatctca aagttaggaa cagcaaacca ggcagcgtct acgattggct tagtatccac
    11521 gattccaaac agcaaggcta cgatgtatcc gctaatgata cctagtaaaa tcggaatcaa
    11581 tccgagaaaa cctcttagcc acatgttgaa gaaaattgtt aatcctaatg tgatcagcgc
    11641 tacagcaata tacttgaaat catacgtgcc ctgattatac atagcatttg ttgctgcatt
    11701 tgccgcaagt cccaacccga taaccatgat gactggaccg acgacgattg gaggcaagat
    11761 tttatccagc catgctgaac cgatcttttt gatgatcagc gcaacaatca aatagactaa
    11821 tccagttgtg actgctcctt gtgcaatcgc tggataacca tctgttttca tcaaaagctg
    11881 catcgctgca atgaaagcga agctgcttcc taagtacgca ggtatctttc ctttagtagt
    11941 aaataagtac accaatgtac ctagtcctga actgactagc gcaatacctg gatcgatacc
    12001 tactaagatg gggactaaca cggttgctcc gaacatcgtg aacaagtgtt gtaaacttaa
    12061 tcctacccaa tgtcctgggg ttgggcgatc gtggatatct aacaccacgt catcattatg
    12121 aaatcctttt ttctccgaca tgtttctctc tcctctaatc ttcctctttc aaaatcatga
    12181 tacgatcttg tccatcaagc tcttgcatct ctactaaaat ctcttccgcc cgagatgttg
    12241 gaatgttctt cccaacataa tctgcacgga ttggcaattc tcgatgccca cgatcgacca
    12301 ggactgctaa tgaaattttt cttggtctgc caaaatccat tactgcgtcc attgctgctc
    12361 gaatcgttcg tcctgtatac aaaacatcat caatcaaaat aacttctttt ccttctaagg
    12421 aaactgggat gtcagaagaa tgaagttccg gttcttctgg attttctttt ttgtcatctc
    12481 ggtataaagt gatatccagt tctcctacag gaatatcgat atcttctagc tgcttcaggc
    12541 gttcggcaat tcttgatgca atgtagattc cacgtgtttt gatacctact aatacaatat
    12601 cttggatact atgatttctc tcgataatct cgtaagtaat tcgagtcaat gctcgcttca
    12661 tcgttacctg atctactact tcttttgctt gcatattcat tccccctact tttttgcata
    12721 aaaaaactcc tgccctaaga ggacaggagg aatgtttaga ctgagcgtat actgatacac
    12781 tgatagctat actatgtcaa tgagcaatct acttatggcg caccatacgt atccttgctc
    12841 ccacccttct tagcctcacg ggactaaact taaaggaatt ctattaagtt ttatgctttc
    12901 gttgttaatt actataccgt tatctaaatt aaaatgcaat gttttttctt aatttttcta
    12961 aagttttttc aaaaacttct ggaagaggtg cttcaaagat catcttttcg tttgtagtcg
    13021 ggtgcgtaaa acctaacatt tgcgcatgga gaaactgacc attgcctttc aaagttttct
    13081 tcggtccgta tactggatct ccagctaacg gataaccgat atacttcata tgcacacgga
    13141 tctggtgtgt tcttccggtt tctagtttta attcaaccag agtaaatcct tcgaaacgtt
    13201 ccaatacagt aaaatgtgtg acagccggct tcccatcttc tcttatcgcc tgcattttcc
    13261 ggttttcctt cgaacggccg attggcgcat tgatggttcc tttttcgtga ggaatctctc
    13321 catggactaa agcaacatat ttgcgcaaag aggtcttatc ttttaattgc tgagctaatg
    13381 attcgtgtgc ctggtcattt ttggcaacca ttagcagtcc ggaagtatct ttgtcgattc
    13441 ggtgaacaat acctggacga ataacgccgt tgatcgtaga aagatccttg atatggtaca
    13501 tcagcgcatt gaccattgtg ccatgcgtat gacctgctga aggatgaacg accattcctt
    13561 gaggtttgtt tatcaccgct acatcttcat cttcgtaaac gatcgtcaac gggatatttt
    13621 ctgggaccac atctaatgtt tctggttcgg ggatcaagac agtaatctcg tctccttgtt
    13681 ttactttgta atttgctttg actggctgtc cattgacaga aaccgctcca tttttcagcc
    13741 acagctgaat ttgcgaacga ctgtaatcgg tgaataaatt agtcaatact ttatcgatcc
    13801 taccagattc atggtgaatc gtcgttttaa gttctgtcat ttttttcttt gatccttttc
    13861 atctaaaata atataaatta atatacaaat cactccacat acaagtgtca catctgctac
    13921 gttaaaaatc ggaaagctca taaaatctgt ctggaacata tctactacat agccaagacg
    13981 aatacgatcg ataaaattgc caatcgcacc agctagaacc aaactcaatc caatcatgaa
    14041 ccagcgatta cctttttcac gatttttaat catcaatgtg agaatcacaa cgactgcaat
    14101 gagtgtcact atagtaaaga accacatctt cccttctagc atactccatg cggctccagt
    14161 gtttcgaata tgattcagtg acaaaatacc tggtataaga tttttcgtct ctccaagcgc
    14221 tagattatca gtgatccacc atttgaccaa ctgatccact cctactagca aagcgctgaa
    14281 tatccaataa ataattaaca aaaaatcaat ccttttctat ttgtttataa atatcacgag
    14341 gtcgtacgat cccgagcaga ttaccatcgg gattcccatc tttagtgata agtacggctt
    14401 ccaaagtccc ttgctcacga aacatcgctt caacctcgta aatatgtgtt ttagctggaa
    14461 caaaatgata atttgtgtac tttccgtttt ggatgatcaa atccaacgct cgtttatttg
    14521 ttaaatcaac cggacctttt tggctttctt ttgctagcca aaacccgata ttacgtaaag
    14581 taatcaatgc tacaaatttt cctttattgt ataacggaaa ttgtgaatac cttttttctg
    14641 ctactatctt taacagttca agtaacggaa gatcttgttc aaaacctgtt acacgttttg
    14701 caaatcgagg cagcacggtt tcaggctgaa tcagttcttg ttcgatcagc tggattcgct
    14761 tgactgtcca atcatttggc tctgcaatga caaaatcgac tgcgatccga tcatggacga
    14821 tcgcattcct taactgagca agctgtaaaa gatcatcttc gtattttctg atcattaatt
    14881 gttttctttg agctaggcgt ctgacaagtt cggtaaatcc catatttcgt gcatttccca
    14941 tctcgtctct catccatttt tcgattcgat tgaaactgat taaaaaaaga tcagagtttg
    15001 ccataatttt cctccttgat caaatatgcc cggtttttta tatagaaaaa gaacgcaatc
    15061 aacacaataa ctgcaaaaaa actgaaaaat aaattttgta ggaaaacggc ccccaaccca
    15121 gttgctagaa ataaaaacgg atagatttga agaatcaagt aattcattgt gatcacataa
    15181 gcaggctttg cttctttcac tgtataaacg gggcttttta gccaataaaa ggtgacctgc
    15241 atcgtaaagg gcacttccag atcaagcgtt atcttttttt catgactgag aacactgcgt
    15301 ttttcatcgt tgattcttag ctgaatcgag ctacccatac cgtaaaaacc ggtttttctt
    15361 ataaatgtcg ttttcatcat accctcctat tatgctttag tatagctcaa aatacaataa
    15421 agaaaaagaa tgatcctagt gtaaacaaaa ttaattccct atcgtctaaa aaaagaagct
    15481 gtagtcaaaa tacagctc
//
LOCUS       DACDNO010000065        14379 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDNO010000065 DACDNO010000000
VERSION     DACDNO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.065"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            202..516
                     /locus_tag="IWI22_002220"
     CDS             202..516
                     /locus_tag="IWI22_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9169592.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            537..914
                     /locus_tag="IWI22_002221"
     CDS             537..914
                     /locus_tag="IWI22_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9169593.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            924..1697
                     /locus_tag="IWI22_002222"
     CDS             924..1697
                     /locus_tag="IWI22_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169594.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1719..3122
                     /locus_tag="IWI22_002223"
     CDS             1719..3122
                     /locus_tag="IWI22_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9169595.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3304..4488
                     /locus_tag="IWI22_002224"
     CDS             3304..4488
                     /locus_tag="IWI22_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9169596.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4485..4775
                     /locus_tag="IWI22_002225"
     CDS             4485..4775
                     /locus_tag="IWI22_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169597.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4772..4993
                     /locus_tag="IWI22_002226"
     CDS             4772..4993
                     /locus_tag="IWI22_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169598.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5035..5814
                     /locus_tag="IWI22_002227"
     CDS             5035..5814
                     /locus_tag="IWI22_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9169599.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            5881..6375
                     /locus_tag="IWI22_002228"
     CDS             5881..6375
                     /locus_tag="IWI22_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9169600.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6431..7069
                     /locus_tag="IWI22_002229"
     CDS             6431..7069
                     /locus_tag="IWI22_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169601.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7141..7536
                     /locus_tag="IWI22_002230"
     CDS             7141..7536
                     /locus_tag="IWI22_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9169602.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7520..9973
                     /locus_tag="IWI22_002231"
     CDS             7520..9973
                     /locus_tag="IWI22_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9169603.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            9970..11997
                     /locus_tag="IWI22_002232"
     CDS             9970..11997
                     /locus_tag="IWI22_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169604.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            11994..13016
                     /locus_tag="IWI22_002233"
     CDS             11994..13016
                     /locus_tag="IWI22_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9169605.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13033..13962
                     /locus_tag="IWI22_002234"
     CDS             13033..13962
                     /locus_tag="IWI22_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9169606.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14207..14323
                     /locus_tag="IWI22_002235"
     CDS             14207..14323
                     /locus_tag="IWI22_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9169607.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         4862 a         2423 c         2865 g         4229 t
ORIGIN      
        1 aaaataccta ggtagtcgtt gttttaaata tagtaggaat cggacgctca gtacggagcg
       61 tctatttttt tacccaattt tgaaaggaag tgaacttatg aaaataaaaa atcaagaatc
      121 aaaaggtcgc tctcccccct ttaaaaccat cacacaaata agattcattc aaacataaaa
      181 acagaaagga taggtaaaaa tatggaactt aaatatgtca ttcccaatat ggaaaaaaca
      241 tttggacaat tagaatatgc tggtgaaggg aacattgaac aacgtcgaat aaatggcaga
      301 ccaaccattc tctctcgtag ttacaattta tactcaacga ttcaacgagc agatgatatt
      361 gttgtcattc ttccctccga agctggcgag aaacattttg aatccgacga aaaagtcaaa
      421 ctcatcaacc cacatattac cgcagaaggt tataaaattg gcaatcgagg atttaccaat
      481 tacattatgc acgcagacga tatggtgaaa gcataagaaa ggaaaggtga aaaaatatga
      541 gattagcaga aggtattgtg attgaaaaag accagacatt tggcacattg aaattttccg
      601 ccttgcgtcg tgaggttcgt atccagaatg aagatggtac ggtttccgaa gaaattaaag
      661 aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat gatacaagtc agtatccccg
      721 cttctgttcc tttgaaagaa tttgactaca acgcagaagt ggaattaatc aatcctgtgg
      781 cagataccgt agcgacggct acttttcgag gggcagacgt tgattggtac gtgaaagcag
      841 acgatcttgt attaaaagga cagacaaaac ctcatcagaa atataaaaaa gaagcgtccg
      901 aaagtgaaaa ataggtgttg cttatgacaa acacaccatt gattttgaaa gaaattccga
      961 aggatgaagc catttctttt attcggcaat accattattc aaagatactc cctagattgt
     1021 gtaaatactt tctagggatt ttttcagaag aaaaattatt aggcgttgtg gaattaggct
     1081 ggggtacgca acccctacaa accatacgca aactctttcc cgacagttcc cttcaaacca
     1141 cagattattt agaaattgga aagatgtgtt ttcttccaga aatgaatcaa accaattatt
     1201 ttggcagtca agctctttca gctctaatta agtggttgaa agaacatacg gactgtcatt
     1261 ttttatatac actggcagat ggaattgagg gaaaatgtgg ctatgtctat caagcgtcca
     1321 acttctttta ctgcgggtat ttcaagacaa gtgtctatcg tgataagcag tcatgggaaa
     1381 agatacaccc tcgaagtgct cgccttttat tagaggaaaa tgctcgcttt gaacaggtag
     1441 agaaaaagca ttggctttcc caggcatttt gcgaatacaa agggattgag aaaatcaatg
     1501 gacgcatgtt tcgctatctc tatccgttga caaaagaagc gaaaaaactg ttaggacaca
     1561 ctctctatcg acgtcattat tatccgaaag aaaaaaattt acgctttgaa aagcgaatcg
     1621 cttatcagaa atatgaagcc atttctcaac caacttttga taaacaggct cgtatctaca
     1681 atacacaatt attttgatag gtggaaagga ggatttttat gccattgctt catgacagag
     1741 gagagcgaat caaagccaac gataagcact tggtctatca ttttgtacta atttcattgc
     1801 tggtagtttt tctagtcgtc ctactccctt ttcattggag agcgttacaa tcggtgaact
     1861 ggcaagcagt tcagttagat acatttttct atcaacttca tacgccatac ttttggagca
     1921 gtattctcac tgctcttttt gtatgcggac ttatcagctg gctttattat cactatcgta
     1981 tagattctat aaaacagtta gagcaccgtc aaaagttagc tcgtatgatt ttagaaaatc
     2041 attggtatga agccgaagaa actcaatcag agggattctt taaagattta cccagcaaga
     2101 aaaccaaaca acgaatccgc cattttccta aaatatatta tcgaatgaaa gatggactaa
     2161 tccatattca tgtagaaatc accttaggga aatatcaaga tcagcttttg cacctagaaa
     2221 acaaactaga aacaggattg tattgtgaat tagtggaaaa aacgttgaaa gattcttatg
     2281 tagagtatgt cctactttat gatactattg gcaatcgaat tggcattgaa gacgtggtag
     2341 ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg ggagttcgat tctctccctc
     2401 acatgttaat tgctgggggc actggtagtg gaaaatccta ttttattctc acattgattg
     2461 aatctctcct gcatacaaat gcaaagcttt atattcttga ccctaaaaat gctgatttgg
     2521 cagacttagg ggcggtcatg aataatgtct attaccgtaa agaagatatg ttggcatgta
     2581 tcaatcaatt ttacgaagat atgattgatc gcagtgaaac catgaaacaa caccctaact
     2641 ataaaacagg agaaaattat gcttatcttg gtttgtctgc caattttctt atttttgatg
     2701 aatatgtggc aatgatggat atgctaggtc gtgaaagtac caccgttatt cataaactca
     2761 aacaaattgt aatgttagga cgacaagcag gcttcttttt aattcttgcc tgtcaacgtc
     2821 cagacgcaaa atattttagt gatgggattc gggacaactt taacttccgt gtggctttgg
     2881 gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag cgatacacaa aaacaatttt
     2941 tcttaaaacc catcaaggga agaggttacg tggatacagg aaaaagtgtg attagtgagt
     3001 tttatacacc ccttgtacca aaaaggtacg atttcttagg agaaatagga aaagtcattc
     3061 aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg tacaggttca gacttgcttt
     3121 gattggtgtg gcgttagcca cgccaatcct ttagccccct ctatctaaca ggggggcaca
     3181 aatacaactg aaaacaggca attctcatca accaatgttg atggtacaaa ggattgcgga
     3241 aaatatgaaa ggtgtaaact caagggtcat agtttacatc ttttttagat tggaggtttg
     3301 cgaatggaaa acgaaaaaat ttggattaag gaattgaaag aaaagcgact ggcttatggc
     3361 gtttctcaaa ataaactggc ggttgcttct catattacca gaccttacct atcggatatt
     3421 gaaacaggca aggcagtccc tactcaacag gtgaaagaag atttactgaa cgctttggaa
     3481 cgcttcaatc ccgataaccc attggagatg ttattcgact atgtacggat tcgattccct
     3541 accaatgatg tggctcaaat tatcggtgag gttttacgtt tgaatatgga ttatatgcta
     3601 cacgaagact ttggctatta ttcttatccc gaacactatc gtttcggtga tattgttgtc
     3661 ctagtctctc atgatgtaag taaaggtgtt ttattagaac tgaaaggaaa aggctgtcgg
     3721 caatttgaaa actttctatt ggctcaacat cggagctggt atgatttctt tcaagattgc
     3781 atggaacata aaggtatttt caaacgcttg gatttagcta tcaacgataa aacagggatt
     3841 ttgaatattc cagatttggc aaagaaatgt aaacaggaag aatgcatttc cgtctttcga
     3901 agctttaaaa attatcgcag tggtgaattg gttcaccgtg atgaaaaacc atacatggga
     3961 aacacacttt atattggctc attaaaaagt gaagtctatt tctgtatata tgagaaagat
     4021 tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg cagaagtaaa gaatcggttt
     4081 gaaattcggt taaaaaatga ccgagccacc caagccatga aagatttatt ggcacatcaa
     4141 caggcggaaa aaacagcctt tgaaattatc aatcgctata tccgctttgc agataaagac
     4201 gacactaaac gtcggagtga ttggaaaact aatgaacgtt gggaatggtt tatcggtaaa
     4261 aatcgtggag cattacgcct aaccacacag ccagagcctt attcttttga acgaacattg
     4321 aactggttac atcatcaagt cgcccctaca ttaaaaatcg caagcatttt agatgttctc
     4381 aacggtacaa ccattatctc caccatgatt caggaagcca aactgacaga aaaacatgag
     4441 aaattaattg aacaacaaca tctagccatg gaggatttga ttacatgagc cttttaacaa
     4501 aagaattaaa gaagttaggg tttcagtgtg gcattgagtt tcaagcgtat atacaaaata
     4561 cagggaaata tacgtctctc attattgagg gaaaacggca agctggtgat accatctata
     4621 cctatgattt ttacaaggtg agattttata ataattatac caatcgagta accgtctatg
     4681 gagagcattt aacccctttt caattattaa ggcgtgtaaa aagctatatc tattatcgag
     4741 aaaaatatct taaggaaagg aggacaatca catgaatttt gggcagaatt tgtacaactg
     4801 gttcttggat aatgcacagt cattggtatt gttagcgatt gttgtcattg gactgttttt
     4861 aggttttaaa cgagaatttt caaaactcat tggcttcttg attattgctc taatcgccgt
     4921 cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta ttaaacttat ttaaccgaat
     4981 cattggtgct taacagatat ttttcatgat aaaataggaa gtatatattt tgttatgaaa
     5041 ggatttatga atatgtttac aaatgatcaa tttaaatctc ttttaattaa aacattagaa
     5101 agaaagagct cttcaaatta ttatgaagga ggtaacgaga ttgtttctaa aatgaaaata
     5161 tcagaggtaa ctttagatga gactgacgat tttacttatt ataaaggata taaaaagggc
     5221 tggaatacgc tttgcacata tcttaaaata agagttcctt ttgatgattt agactttttt
     5281 gaaagtcata aagatttgat tacccagaca gcaagctcca tatatgataa acaaggtgat
     5341 aatgtattag tggatactat tttagtacca cttccagaga attacgaagt aattaacttt
     5401 agtcagttga aaattagtga tgtggtttca caagctattg aagacgctga gtcttttatg
     5461 agtaatggtg aatatcaacg tgcctttgac agagttcaca ctgcttttca tggatattta
     5521 attgaaatac taaaaaaata tgaaattaca gttcctagag acgaaaatct atctaaatta
     5581 tatagtagga tacaacaatt aattgaaaaa gaaattcaac ctactgaact tgctgatata
     5641 gtgaagacaa cgataaggtc atcaaatgga atgattagct ctcttaatga agcaagaaat
     5701 aggcattcac ttgcacaccc taatacaaat attattggaa agagagaagc aaaactaata
     5761 ataggtattt caagtacagt tacagattat attagcggat accttgataa gtagcgtcat
     5821 taactgattg ctactttttt tatgactatt tttataaaaa ggatgtgata agaattggaa
     5881 atgaaagtct atgtggcaaa tttaggacgg tataatgaag gtgaattagt cggagcatgg
     5941 tttactccac caattgatga ggaagaaatg gcagaaagaa tcggtttaaa tgaggactac
     6001 gaagaatatg caatacatga ttttgagctt ccttttgatg tggacgaata cacgccgatt
     6061 agtgaaatca accgtctatg tgaagccatt caagaaatcg aaggtacacc tatttataat
     6121 gaactcaaag aaattcaagg catgtggttt agtagcttgg aagaattact ggaaaacaaa
     6181 gaggatattc attgttatag tgattgtgat tcgatggaag atgtcgcccg ttattacgtg
     6241 gaagaaacag gacagcttgg tgaagtccct tccaatttac aaaattatat tgactaccaa
     6301 tctttgggac gtgatatgga aatagagggt aattacctag ttacctctca tggggtattt
     6361 gaatattgtc agtaagttta acaatccgtg ttataattaa attaataaat agcacggaga
     6421 ggtataaatt atggtaaatg tatttgaaaa tcttaaacta ttaaaactag acatgaaaga
     6481 taaaggctgg gtgatagatt ccttttactt tcgttacaaa cagcaaaatt atattgtact
     6541 tgttaagcta tttgaaaaag aggaaaaagt tccagaatat gctctgttaa aactagaatt
     6601 tttaaaggaa aacgattttt cagatatgct agctgtctat gctaattcgg taaaattata
     6661 tacagacacg aaaacaataa gagaatattt tggtatcgaa tacagtagta acttgggaga
     6721 tgttttattt caatttagcc aaactcttgc tcgatttatt ccgacagaag ttagtgagaa
     6781 gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta agtcaaagtg attcagaaga
     6841 tcccagaaaa aaatattgtt tttcagttag aagaaatcct ttaaagggaa atggtgagct
     6901 gggaaaacga agtcctttta atgacaataa aacgagattg tatagaccca gcttatatga
     6961 acgattaggg tcagatacca atttaagttt cagatattca atgaatccag atgatgaaga
     7021 aactgatgaa ggtattattg caaaatggac aaaaaataaa attgaatagt taaagcattg
     7081 aaagagcaat cacctatttt gattgttctt tttctatttt tacggaaagg acgtgaagac
     7141 atgaaaaaaa taagaagtta caccagtatt tggtcagtag aaaaagtgat ttatgcgatt
     7201 aatgatttac aactcccttt cccaattacc tttacgcaaa tgacatggtt tatcgtttct
     7261 ttgtttgtcg tcatactctt tggcagttta ccaccacttt ctctgattga cggagcacta
     7321 ctcaaatact tagggattcc cattgttctc actgggttca tgtgccaaaa gacctttgac
     7381 ggaaagaaac cctatggatt tctaaagtct atcattacct atgctctacg ccctaaagta
     7441 acctacgctg gcaaacaaat acgattaaaa aaagaaacaa tgaatgaatc gattacgatt
     7501 gtaaggagtg aacaactaga tggcatatcc gattaaatat atggaagata atctcgtatt
     7561 taatcatgat ggcgaagtat tcgcttatta tgaattaatt ccctataatt acagtttttt
     7621 aagttccgat gaaaaaattc aagttcatga taatttctgt caattgattg cccaaaatcg
     7681 tgatggcaag attcatgctc tgcaactagc aaccgaatca agtattcgag ccacacagga
     7741 acaatcgaaa aaagaaatca ctggacgatt aaaagagata gcttacgaaa aaatagatga
     7801 acagaccgaa gcgttaattt ctatgattgg tgacaatcaa gtagactatc gcttttttat
     7861 tggttttaaa ctctacctca atgaacagga agtatccgta aaaaatgttg ggaaagaaat
     7921 tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac aaattaatgg gcgattttgt
     7981 ttctatgcct atggcggaaa tcgaacgctt ttctaagatg gaacagttgt tagaaagtaa
     8041 attatccaga cgctttcaga ttcgtaaatt ggagaaagat gattttggtt atattattga
     8101 acatctttac gggcaaacag gtgtcccttt tgatgactac gactaccatt taccgaaacg
     8161 atacgaagat aaaaaagtgt tggtaaagaa atacgatatt ctcaaaccca ctcgctgttt
     8221 aatcgaagaa aatcaacgct atttacggat tgaacaggaa gataccacta cttatgtggc
     8281 gtattttaca attaatagta ttgttggtga attggatttc cctaatagtg aaatttttta
     8341 ttaccagcaa cagcagttta ctttccccgt tgataccagt atgaatgttg aaattgtcgc
     8401 caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa gaactgaaag atttagatag
     8461 ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg gtggacgctt tagaatctgt
     8521 cgatgaatta gaaagtaatt tagaccaaac caaagaatct atgtacaaac taagctatgt
     8581 ggtacgggta tcagcaaaga atttggacga gctgaaacaa cgttgtgatg aagtaaaaga
     8641 tttttatgat gatctgtcta tcaaattagt acgacctttt ggcgatatgc taggcttgca
     8701 taatgaattt attccctcaa gcaagcgata tatcaatgac tatattcaat atgtcacgtc
     8761 ggattttttg gctggtcttg gtttcggtgc tacacaaatg attggtgaaa attctggtat
     8821 ttacgtcggt tacaatctcg atacaggcag aaatatttat ctccaaccta gccttgcaag
     8881 tcaaggaatt aaaggctctg taacaaatgc attagcgtcc gcttttattg gttcattagg
     8941 tggcggaaaa tcattctcta acaatatgct ggtctattat tcagtactct ttggcggaca
     9001 ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg aaagaaacct tacctgatat
     9061 tgcccatgaa atcaatattg ttaatctcac gagtgagaac gacaataaag ggttacttga
     9121 cccttatgtg attttaaaac tcccgaaaga ttcagaaagc cttgccattg atattctcac
     9181 gttcttaaca ggtatttcca gccgagatag cgagaagttt cctgtcctgc gtaaagctat
     9241 tcggagtgta actcaaagca aacaacgagg gttgttattg gtgattgatg aattgcgaaa
     9301 agaagatacg cccatcagca atagtattgc tgaccatatc gaaagctttg tcgattatga
     9361 ttttgcccat ctgttattca gtgatggcag tgtcacccaa tccattagct tggaaaaaca
     9421 actcaatatt attcaagtgg cagatttggt cttacccgat tccaatagct cttttgaaga
     9481 atacactacc atggaattat taagtgtggc aatgctgatt gtcattagta cctttgcttt
     9541 agactttatc cactctgacc gttccatttt taaaattgtt gaccttgatg aggcttggag
     9601 ttttttacaa gtggcacaag gaaaagctct atctatgcga ttggttcgag caggacgtgc
     9661 catgaatgca ggggtctatt tcgttactca aaacgcggac gatttactag atgaaaagat
     9721 gaagaataat attggcttga aatttgcctt tcgttccact gacctagtgg aaatcaaaaa
     9781 gaccttagaa ttttttggtg tggacaagga agatgaaaac aatcagaaac gcttacgtga
     9841 attagaaaat ggacaatgtt tagtttcaga catctatggt cgtgtagggg ttatgcagtt
     9901 tcacccgatt tttgaagagt tgcttcatgc ctttgatacc agaccgcctg tgcgaaatga
     9961 ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact atagttggat aacgctcctt
    10021 tgtgtgggcg ttattttact tttattaggc acgctgggca atgcagtaca agcgacaggc
    10081 ttagttgatg aaaccattga tacaagcaat gaatactcca agtatggttt gaatcaatat
    10141 cagcttgatt attatgtgga caatagctgg ggctggcttc catggaattg gagtgatggc
    10201 ataggacaat cggtgatgta tggattgtat gccattacta attttatttg gacaatttct
    10261 ctttatctat cgaacgccac aggttatttg gtgaaagaag cctatgcctt agatttcatt
    10321 agtcaaacat cccaagcaat tggcgaaaac atgcagacga ttgcaggcat tacaccgaat
    10381 ggcttttcca gttcagggtt ttatgtcggt ttcctccttc tttttattct cattgttgga
    10441 atctatgttg cctatacagg attggttaag cgagaaacga cgaaagtgat acgagctgtt
    10501 ttaaactttg tcgtggtgtt tattctatcg gcttccttta ttgcgtatgc acccgattat
    10561 attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg ccagtctgaa tattgggaca
    10621 aaaattgttc ttcctaacac ggatagtcaa gggaaagaca gtgtggacat gatacgtgac
    10681 agcttattct ctgttcaagt aaagcaacca tggctattgc ttcaatatgg ctccactgat
    10741 gtagaagcgt taggggaaga acgtgtggaa agtttattgt ctaccagtcc agatacaaac
    10801 aatggtgaag atagagaaaa tgtagtgatt gaagaaattg aagatcatga caatacctat
    10861 ttaaccctac cgaagacgat ttccagatta ggaacagtct tctttttgtt tgtattcaat
    10921 attggtatct cgatttttgt tttccttcta acaggaatca tgattttttc gcaagtgcta
    10981 tttattattt tcgctatgtt tttgccaatc agtttcctat taagtatgtt gccgagtttt
    11041 gatggcatgg gcaggcatgc cattaccaag ttatttaatg tgattatgac aagggctggt
    11101 attaccttga ttattaccgt tgcctttagt atttccacta tgctttattc gctgtcagca
    11161 agttctccct ttttcatgat tatgttttta caaattgtga cctttgcagg gatttatttt
    11221 aaactgggcg atttaatgag cctattcact ttacaaagca acgactctca aaacatgggc
    11281 agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac aaaccagaag atttcaacgc
    11341 agtatgagta agatttttcg tggaaaagca gggtcaacga agcaaaagac aatgacaaca
    11401 aagcagaatc aaaccaataa agctaaccat actcgtcaaa atgaacggac tacaccaaag
    11461 aaagaaacag cactttccaa tgtgaaaaag caacaaggcg gtatcgggca acgattaggt
    11521 gaaaagacag cccatgtatt ggacacgaaa aatcgtctag tcaataaaag aaagcaggca
    11581 aaacaacaga tacaggcgac accaacccat gtcaaatatg cccttcatca aggaaaagag
    11641 aatgtcactc ataatgtaac agactttaaa caatctatgg cagataccaa agctcaacgc
    11701 aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga acattgctca aaagcgtttg
    11761 gagctggata aagcaaaaga aaaaagacga aaagaaaacc catcatctcc tgttcagaat
    11821 aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc ctgttttgtc acaaaacact
    11881 aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa aacaaaagac accaccgaag
    11941 caattcaatc agaggaagct aaataaaaga acggataagc gaggacaaca aacatgaagc
    12001 taaaaataac agtgatttgt agtgtggtct tctctctctt cttttttctc ttattattcc
    12061 tagtcatttt ctttgcagat gatacggaca gtggcgaaaa caacaaggat tcttctattc
    12121 cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca ccgtccgttg attgaaaaat
    12181 atggtaagga atatgggatt gaagactatg tttcttatat cttagccatt atgcaagtgg
    12241 aatcgggcgg tacagcagaa gatgttatgc aaagttccga atctttgggt ttaccgccca
    12301 acagtttgag taccgaagag tccatcaaac aaggggttaa atatttcagt gaattattaa
    12361 ccagtgcaga acaacaagga gtggatatag attctgttat ccaatcttat aattatggcg
    12421 gtggcttttt aaattatgtg aggagtcatg ggaaaaaata tacctatgaa ttagccgaac
    12481 agttttctaa agagaagtcg ggtggtcaaa aagcagacta tcccaatcct atagccatac
    12541 ctgtgaatgg tggttggcga tataactatg gaaatcagtt ttatgttcag ttggtttcac
    12601 aatacctaac cgacacttcc ccaacagaat ttgatgatga aaccgtgcaa gtcattatgg
    12661 acgaagcctt gaaatacgaa gggtttccct atgtctttgg aggggcgtcg cctaccacct
    12721 cttttgattg tagtggctta atacaatggg tgtatgacaa agctggcatt tctctccctc
    12781 gtgtcgccca agatcagtat gacgctacgc aagaaatctc tatggaagaa gcccaagcag
    12841 gcgaccttat cttctttcat tcaacctata atgcaggcac atatgtgact catgtggcga
    12901 tatatttaga gggaaatcgc ttttatcatg caggcgaccc aatcggttat ggcgacttaa
    12961 gcagtcgtta ttggcaagac catctgattg gggctagacg tgttattcat aactaagaaa
    13021 ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa agaaggagaa accaaagaaa
    13081 tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta aagttggcac gcataagaaa
    13141 ttaacctttg ttttatgggt tttattgatt ggtagtgtcg gatttggaat ctataaaaat
    13201 tttactgcca ttgacactca tactgtccgt gaaacagaaa tcattaaaca gcagattgtc
    13261 gataccaacc aagtagaaag ttttgtgaaa tcctttgcga aagactactt ttcgtggcaa
    13321 caatcccaag aagccattga taaacgaaat gaaaaattga cccattattt gacggaagaa
    13381 ttgcaggtat taaatgaaga aatgattcgt aaggatattc ctactagttc tagtgtgaac
    13441 gatatacagg tgtggcaggt ttctcaagtg aatgagaaca cctttgaggt actgttttct
    13501 gttgaacaag ttattaccga agacaaagac aaggaaacta tatcgtctag ctttcatgtg
    13561 gttgtccata tagatgaatc agataatatg gttattatca aaaatccgac aatgagtaag
    13621 aaacctcaaa aatcggacta ccaacccaaa cagctagaaa gtgaccatac agtagataca
    13681 gaaacgatgg acgaaatcac tagcttttta gaaacgttct tccagctcta tcctaccgcc
    13741 acagaaaaag aattgactta ctatgtaagt aatcatgtgt tacctatgat taacaaggaa
    13801 tatgttttcg aggaattggt gaatcctatc tttaccagaa aagataacca agtaatcgtg
    13861 aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa cccaaatctc gcaatttgag
    13921 ttgatattag aaaaacaaga taattggaag attgtaaaat aacaaatatt ggtacatgat
    13981 tacagatact ttgtaatcat gtactctttt tgataaaaaa ttggagattc ctttacaaat
    14041 atgctcttac gtgctattat ttaagtgact atttaaaagg agttaataaa tatgcggcaa
    14101 ggtattctta aataaactgt caatttgata gcgggaacaa ataattagat gtcctttttt
    14161 aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga ttctaaagta
    14221 tccagagaat atctgtatgc tttgtatacc tatggttatg cataaaaatc ccagtgataa
    14281 aagtatttat cactgggatt tttatgccct tttgggtttt tgaatggagg aaaatcacat
    14341 gaaaattatt aatattggag ttttagctca tgttgatgc
//
LOCUS       DACDNO010000066        14236 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDNO010000066 DACDNO010000000
VERSION     DACDNO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.066"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(232..438)
                     /locus_tag="IWI22_002236"
     CDS             complement(232..438)
                     /locus_tag="IWI22_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169608.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(454..1068)
                     /locus_tag="IWI22_002237"
     CDS             complement(454..1068)
                     /locus_tag="IWI22_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9169609.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1215..1898)
                     /locus_tag="IWI22_002238"
     CDS             complement(1215..1898)
                     /locus_tag="IWI22_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9169610.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(2007..2465)
                     /locus_tag="IWI22_002239"
     CDS             complement(2007..2465)
                     /locus_tag="IWI22_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9169611.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2549..4312)
                     /locus_tag="IWI22_002240"
     CDS             complement(2549..4312)
                     /locus_tag="IWI22_002240"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9169612.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4533..5993)
                     /locus_tag="IWI22_002241"
     CDS             complement(4533..5993)
                     /locus_tag="IWI22_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169613.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYLEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6201..7127)
                     /gene="glsA"
                     /locus_tag="IWI22_002242"
     CDS             complement(6201..7127)
                     /gene="glsA"
                     /locus_tag="IWI22_002242"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9169614.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(7150..8577)
                     /locus_tag="IWI22_002243"
     CDS             complement(7150..8577)
                     /locus_tag="IWI22_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9169615.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8608..9822)
                     /locus_tag="IWI22_002244"
     CDS             complement(8608..9822)
                     /locus_tag="IWI22_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9169616.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(10163..10405)
                     /locus_tag="IWI22_002245"
     CDS             complement(10163..10405)
                     /locus_tag="IWI22_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9169617.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10437..10994)
                     /locus_tag="IWI22_002246"
     CDS             complement(10437..10994)
                     /locus_tag="IWI22_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9169618.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(11007..11195)
                     /locus_tag="IWI22_002247"
     CDS             complement(11007..11195)
                     /locus_tag="IWI22_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9169619.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(11208..11774)
                     /gene="amaP"
                     /locus_tag="IWI22_002248"
     CDS             complement(11208..11774)
                     /gene="amaP"
                     /locus_tag="IWI22_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9169620.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            13004..13477
                     /locus_tag="IWI22_002249"
     CDS             13004..13477
                     /locus_tag="IWI22_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9169621.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            13486..13713
                     /locus_tag="IWI22_002250"
     CDS             13486..13713
                     /locus_tag="IWI22_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9169622.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         4256 a         2814 c         2283 g         4883 t
ORIGIN      
        1 gttattacta tattctattt acgagttcat gatagacttt tcctaatgat taggcaatgg
       61 aatttgaaat gaaatagtga aatttaacaa tctttaatat tttttagaaa aaaataacaa
      121 ataagttgaa agaatcccgt tgcaaattct ttcagttgca ccaggattct ttgataattt
      181 tttagtttta tgtgtttttt tatatgtttt ttcacttttc ggaataaata tttatttgtt
      241 aaagtaatta aacaatccgc taatcgtttg atctggcatt tctatttcct taccatctaa
      301 agtaacagta gtaacgacaa aatggtcttg caatggcgga acttctaagt actgcccttg
      361 cagaaaatca ttcactgttt catattcttt tttcatttct tttgaatctg tcgatggttt
      421 atagcttatt tttaacataa ctagtgctcc tttttattcg ggacgtgcaa tcgtgaaaat
      481 ttctcctatt gctgcataat catttcctgc caaacggctt acagcattca attttcttgg
      541 atcgatcctt cctgtcttat catcatagac tgtttcattt aaatgatatt tctgtacttc
      601 tagcaaaaat aaatcagctg ttactttatt tttattccgt atctcgacat gttcatataa
      661 ggatgtttca aaacgaactt ttgcttctgt caatgctagt actgaaatga ttactgaatc
      721 agttaaagtc aagtcagtca ggtacagttc gctttcctca ttaggcagat tggctgctgt
      781 tttatttact tcttctacgt tgtcctcatc aaggatatgg ataacagctt ctcttgtctc
      841 aagaatattc tttgctgtgt cttttactgc atcttgtttt ctctggatcg ccaaggataa
      901 gatgggtcga ttcgaactga cgatattgaa gtaactgaag ggtgcgatat tcactatatc
      961 ttttgctgat ttagtcgtta ctactgctac tggtctaggg atgactgaac caatcagcaa
     1021 cttgtaattt tcacgttctg tcaattctga aggactccgt tcaaacatgg tttcgccttc
     1081 tttctataaa attcgttaaa aagcagatct tgaagacaaa atgaaaagta atgctttttt
     1141 catcttctta gaatctgctt taaactatct atcaattttt cttttttgtc aagaatctgt
     1201 gccgtgcgtg ttttttagtt atttgcttta cttgcggctt cgacatatct tttagtgatc
     1261 aattgcggac gggtgataat agatccaact acgactgaaa aagcacctaa ttcatatgct
     1321 ttctctactt gttcaggaga gtcagtatgt ccttccataa ttactgggat atcgacttgt
     1381 tctatgattt gattcaataa atcaaaatca ggtgtctgcg tctttcgtga atattctgta
     1441 tagcctgcta aagtcgtaga caaaagatca aaaccatctt cttgcgcttg aagcgcttcc
     1501 tcaaaagtcg aaatatctgc catagctaac cgaccgtgtt cgtgaatcat ttggacaagt
     1561 tcagcagttg ttttttctgt cggacgttta cgagaagttg catccagtgc aatgatttct
     1621 gcttgcgtct ccaaaagttc ttccacttct ttttctgtcg cagtaataaa cacttctgac
     1681 ccatcgtaat ctcttttgat aatccctatt accggtaaat caacgattcg cttgatttca
     1741 ttgatatctt ctgtcgtatt tgctcgaata ccaactgctc cgccttcttt tgcagccaaa
     1801 gccattctcc ccataataaa agaactgtgt agcggctcgt tttccaaagc ttgacaagaa
     1861 acgacgactc cgcctttgat ttgttccagt gtatccacaa tatctcctcc agtaatgtta
     1921 tataaaaata cagcttcata taaaaatgta acccttttct attcaggaga caataatgag
     1981 ctatttcttt cagcagacct ttcttactaa aagaaaatta cttcaaattc ttcgtatacg
     2041 accagcttct cttctgtaaa ttctatgttc aacgcttctt tataagcatg tttaaaaaac
     2101 tcagcatgga ctttccgcca ataagctaat gtccgatccc cttctccttc tttccaagca
     2161 tgctctttcg tgacttcact aaaaggcaag atggtcacat ttatattttt gatgatacaa
     2221 actggttcat tttttccatt caggaccacg tctaattgac caacttttgg taaaggctca
     2281 ttctcttgtt tatacagatc gtaaccactt gatgtaccag tctttatctt ttttacaact
     2341 agttcgctca actcgtctgc cattttttct gtatccccaa atgaaaagaa acctaatttt
     2401 tctgattgat aactaggata tttttcttgg aactttttga catattcttc tacttgctga
     2461 tacataacat ccaccccttt gctttttata atgaaaaaac catccagata ctagtataaa
     2521 caattatctg gatggtgagc ataatgaatt attgaatatt caagacacgc agactatctt
     2581 caacgatttg ttttggtgtt aaatgataac gttcataaag ctgccctaca ggaacgctat
     2641 ccgtgaattc tttctctgca ccaaaattta atacgttcat tgaagaatca ccataataac
     2701 gactgatatt ttcgccaaat cctcctgaca aactaccatc ttctaatgtg atgactaatt
     2761 cgtgtgtttc ttttaattta tctaatgttt ctacgtcatt ttctgtgatc gttcctggat
     2821 tgatcaacgt tgcttcgatt cctttttttc taaaagcttt tactgtttct tttcctaatt
     2881 caagaaaacc gcctaaagct aaaattgcta cttttcctcc attatttact gtttgatagg
     2941 ctggaatact ataatcattt gcgatagctg taccatgttc tacaccatgt gtaggaattc
     3001 ggattgctac aggctgcgct gtttgttcga acgcccaatc tagcatagag atcatatcct
     3061 cttttgttgc tggagcaaga tatttgatgt tcgggatact tgataagtaa ggaatgtcaa
     3121 atgttccttg gtgtgttcgg tcactagctg agatcgttcc gccaccgact aaaataatca
     3181 ctggattctc gttgatcgct aaatcatgtg ataattgatc atatgcccgt tgcaagaaag
     3241 tacttgattg gaagacgact ggacgtactc cctgagaagc caaagcagaa gcgaaagtga
     3301 tagagtgctg ttcagcgatc cccacgtcaa aataatgatc tggatgtttt tcttggaatg
     3361 ttttcagtcc aaaggcgcct ggtatcgctg cattgactgc ggcaatagga gctgtacttg
     3421 tttgcatttt cttatccaaa taatccatga tgatcatatt aaatgtttct ccagaagtta
     3481 cttgctttgc ctccccggtg gacagatcaa atggtacatg ccaatgatat ttcattttat
     3541 tatcgattgc cggctgataa ccgcgacctt tttccgtatg gacgtgaagt acaatcggat
     3601 gatcaatatc ttttacttct tcaaataaac ggatcaccgt ttccagatcg tttccattat
     3661 ccacgtaacg ataatcaaat cccattgctt taaataggtt attgctgctt ttaccgttgg
     3721 tttgacgaag ctcagctaag tttttatata aactgccatg attctcatca atagacatct
     3781 cattatcgtt tactaatacg attagattgg attgtaaaac accaccattg ttcaatcctt
     3841 caaaagctaa gccaccactt agggagccgt caccgataaa tgctaccaca tttcctgatt
     3901 ctttcttcag atctctcgct ttagctaaac ctgttgctaa gctgatagat gtagatgtgt
     3961 gccccactgt aaaatagtca tgtttacttt catgttggtt ggaatatccg ctcacttcac
     4021 ggtaatgtcc tttttcaaaa ccattttttc gaccagttaa aagcttatga ccataagact
     4081 gatgggaaac atcccaaacg attttatcct ttggtgaatc aaatacataa tgaaacgcaa
     4141 tcgttaattc tactacacct agattcggac caacgtgccc acctacatcg ctgtcctttt
     4201 gcaagatttt ttggcgcatc tcttctgcca actgtttcat ttcctcaaca gataattttt
     4261 tgacatctgc ggggctattg attgcatcca atacatgtaa tactgtactc ataatacatt
     4321 ctctcctccg tactatcttc ttttaatgaa aatagatttg ttacacttta tctattctac
     4381 tacttagagt tgactctaag tcaatagcat tttaaaaaaa tttcagagaa agcttgttat
     4441 ataagtagat cttcgcacga atcaggactc aaataaaaac cttctacaga caattaaaaa
     4501 gctttgtaga aggtctccta attaaggtct catcaagaaa tatgtttttt taataggtga
     4561 agttcctgag caatatgata caaatggatg tttgcacctt ttaatgagat tggatagatc
     4621 tttgtctttt cagtttctaa tccattttga tagctagcag catcgcacaa aaccactctt
     4681 tttttcgcac atagacattc atcaggcaat tcttctaatg aacgaaataa aataggattt
     4741 atttcagcga tgccttcttc cgcatacaaa tgaaattggg ttcgataaaa aaattgtttg
     4801 actgaacttt ctgaaacaac aaaaatttct atttcctgtc tccccgcttg ttcactgttg
     4861 atcaaagaaa gacttttgac gaattttcga atgaaatttt cattccatct tagttggatt
     4921 tgatttttct ttttccactc ttccaatatt tctacaattt ttccgtacag aagttgttct
     4981 ttttctgata gaagatagat cctttccggt aaaaatactt gtccatctgc ccacaacgta
     5041 cggacaaatt gtaaaaagga ttgcttgaaa ataaaatttt ctgcatcgtc gatttcagcg
     5101 atactaataa tctgtaaaat caattcattc agtgataggt cattgagaac aaaattttta
     5161 taaacaactt cgaaatcttt tcttaacagc tccatgttct cattggtata atttctactg
     5221 ttgaacaaag aaaaaatata atagacatct tcttcttgta agtctagaat tcctttttgc
     5281 tcttccaatc cttgacagac taattgaaat aacggcttcg tttctgcgct tttcttatct
     5341 tcttctttca gtccaagggg aaatgccttt cttccgattg cgatctctat ttctcgtatg
     5401 ataaaggact tttcatcttc caaatatctt cttttaaaaa aatgagtctc tacataatca
     5461 atcaactcgt tacaagcttc ttctacatac ggatttttat tttcatagcc ttcccaattg
     5521 acataacgta ctaaagctag caagatgttc cgcgagtcaa attctgggat aatgatttct
     5581 ttatttttca aataatcatt ttctttaaaa aaatccaata cgattttttt gattgtatat
     5641 actttactca aagaaataaa ttcaagatca gccagttctg tactcgtgaa tataccatta
     5701 aaataataac ttaaaaactt taaaaaataa gattgaccgt acagaaactg tgtgagctca
     5761 gaaagagcaa taccatgttt gaatacgagt ttatacttat gcttctctct tacttgttct
     5821 tttatacaat attctagaca ctcggctaga ctttctagat cgtgaaggat cgttggtgta
     5881 gtcaccctta gagaaatagc tgtttgttcg atgtctattt cttggaattc aaataattgt
     5941 tcacacagat acacttttct aaatatattt cgctcgatgt aattttccag cattcctgat
     6001 acacgcctcc agtttttctt atgattttat ttaatcatat ctgtaaatcg tatttttgtt
     6061 tcctttttat ttagaaaagc cctttcaaaa accaataaaa aaagaaactg ggacaaaagt
     6121 caaacaagct cacaatttca gcctcattgt aggtagcttt gagccgtttg gacttttacc
     6181 ccagcctctt tcaagaatgg ttattcaaag atgtcagcgt ctagttcttt gactacatct
     6241 ttcaacaatt ggataccagc tgcgctgtta ccaaatggat ctagtgccgg gctgaagaca
     6301 cctatgccgt aacgatttgg cacagctgcc atcaacccgc cgcctacacc gctttttgca
     6361 ggtacaccaa catggacgga aaactctcct gattcatcat acaaaccagc agttgtcata
     6421 attgatttga cgattgtagc actttcttca gtaatgattt gtttgccatt ccaaggagca
     6481 attcctttat ttgccagcac agcagctaat tttgctatgc ctgtagcggt gacattgagg
     6541 gagcattgtc tgaaataagc atcgagtatc tccggtacgt ctccttcgat catttgatta
     6601 cctttcatat agtaagcaag agagcgattg atatcacctg ttcttgattc agaatgatag
     6661 atttcttcat tcaatgtgat attaggatca tcacagatct ctttcataaa tgacaagatt
     6721 cgctcaaatt tttcatctgc atctttaccg gcaatcaacg aggttgttgc aatcgctcct
     6781 gcattaacaa aaggattcat tggatgtttg cgatgattga tttccatatt catgatagaa
     6841 ttgaaggcaa aaccggttgg ttccgtgttg attgtttgaa agactttttc tattccgttg
     6901 tctttgattg ctaataacaa tacgggaact tttgacatgc tttcgatggc aaagcgcaca
     6961 tcggcgtctc ctccctcaat cagttgtttt gtatccaaat cataaatcgc catacctaat
     7021 tgtttgggat ccacttcagc taatgctgga atgtaatccg ccacttgtcc tgtttgataa
     7081 tagtggaaat tttttccaat caattgttct aattctttta cttccatttg aataaccagc
     7141 tttcattttt tatttttctg catgctggaa tttatgacgg catgcgtaga tcgtaaatgg
     7201 aataactaag acgataagat aagaaacaat cagaatcgtt tcataaatcc catgctgtcc
     7261 agctgcgata ctagcaggtg gaacaaatga gatacaaaaa gcaaaaagtg aagtcaaaag
     7321 acctaaacaa gcaacgatta cttttacaac ttttccgcct ggaacctgat aggctctttc
     7381 aagactgtca tctttaaaaa ccagcttcaa atagcccgcg aaaaacataa agtacaacat
     7441 caggtagatt acaacagtca atgacatcgc tgcaagaaat gaaagattat tccccccgcc
     7501 gcttaatgta aagacggctg cccagattgt tgcaacgatc ccatttgcga tcatcaaatg
     7561 agcaggaaca ccgtgtgtgt tttcttttgc aaaatattca ggaagtaatc cttcttctgc
     7621 tgctacgtga agtgcttttg aaggagaggt tacccaagaa gcaatttctc cgatgacacc
     7681 taaacaaaca agaatcgcaa gaattttcgc aatccatgca agatttggat caatgtgtct
     7741 taataggaat tctaacgatt gaatcacccc tgtattaaga gacaaaccat gttgaggtac
     7801 tgtagtagcg acggtcaatc cgccaaaagt atctaaaatg attgccagaa taaccaatac
     7861 aaacattgct aatggataat cctttttagg gttttccatt tcatttgcat gactggcaga
     7921 tgtttctacg cccatatatg ctaaaataaa ggaaacaaaa acaaccagcg taggcaattt
     7981 cgaaaaatca ggaatcaatg ttttcataga cagtatcgtt tgtagtggag cgcctgaagc
     8041 gacataatgg atccctaagc caaacaagac taatgctggc aacaagatac cagctacaaa
     8101 accgatccga gcaatcaatg aagtgtattt ggttcctttt aactgtgaga gtaaaatagc
     8161 ccagaaaata atcaatagga ttgccaattt cagatattta ttgttttgta tagcaccaat
     8221 acccgttgca taggataatg caccagttaa gaaatacagc atggtcaagt agccaactgt
     8281 tatctcaaac cattggaaaa agattgcggc aaaaccgaat cgttttccca atgtgcgact
     8341 gacccaagta tagacaccgc ctttttccca tcctttcacc gttgccatct ctgctgcgca
     8401 caatgccaca gggataaacc atagcaaacc gcctaataac aagaaaaata ctaagctgaa
     8461 gcctgaggtc gcgaatgtgg gatattcata taatgaaacg accattgaga tggtcatagc
     8521 caaaaagcca aagaacgtta atttttttgc aggaattttc gttttgcttt gttgcatgaa
     8581 tcttcctctt ttctatttga atttgtttca attgagtttg ttgccaaaga agaaaaagtc
     8641 caatccttct tcttgttctt cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt
     8701 taaaatcact gttgtcagca cataggcata cacggcagta atgatgacac caatcacttg
     8761 aatccctaag aagtaccagc tgcttggatt cgttagtaaa tggctgttag atgcaaagat
     8821 ccctatcaga atcgtacctg tgaatccgcc catgccatga acaccccaca catcaagtgc
     8881 atcatcccat tgtttctttt tacgaaaatc aacagcagca tgacagacga tacccgcaat
     8941 gatcccaatc agcatggcac tttttgcttc tacataaccg gcacaaggcg taatggctgc
     9001 caatccagca acggatcctg ttagtacgtc gacaaaatca aagccatttc cttttacttt
     9061 tgcaatgatc agccaagtaa tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc
     9121 attgactgct tgttcatctg ctcgaagtgc accaccggaa ttaaaaccga accagccaaa
     9181 ccaaagcagt cctgtaccga tagcagctgc cataagactg ctatgctgac ttctttctcc
     9241 taaaggaatc tttctttgac caagtgtgag gatacatgct aaagagccga atccagctgt
     9301 cgtgtggatg actgctccac cagcgaaatc tctaaatccc atctgatcca aaaatccgcc
     9361 accccatatc cagtggcaaa cagggaaata aatcagcaga ttccaaaaaa taacaagtat
     9421 aatatagcct tttaaattca atctccctgc aaaagctcct gtcatcaaag gaacggtaat
     9481 cacgataaac atcagttggt aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa
     9541 catgttgggg aagcttgtaa catgtctcat caaaaagaaa tctgccgggt ttccaataac
     9601 accgccgata tcccgtccaa aggccaatcc gaatccacca aatacccaca taatcgttgt
     9661 tatcccaacg gagagaaaag attgagacat gatcgttagt atattattct tccgtgctaa
     9721 tccgccgtaa aaaaaagcta gaccaggagt catcaggcaa accattgctg tacataaaat
     9781 catgaatgct gtatctcctg tattgatagg taatgtattc atttttttcg cctcctttct
     9841 acgattacga gtataaaaga aggtgtagtt gtttttttct tctgtttttt tgaattaata
     9901 cttgcattta taaaagacag acaattaata aaagcttgat gtgttgtgct ctttttcgtt
     9961 tttcacacaa ataaagacaa aaaataaacg gtcttctttt tatagaaaac cgtttatttt
    10021 cgtctttttt tatttttaaa gatatttttt cataaggaat aaagaatact tcatataaaa
    10081 tatataaaaa agaaccgcag cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa
    10141 cataagtttt tcttcaattt gcttataatt tacgtgcaaa gaatgaaacg actaatacta
    10201 aaatgattgc accgatcaat gatggaatga tagccattcc tgcaattgtt ggtccccaag
    10261 ttcctagtaa agcttgtcca attgctgaac ctactaaacc agcaatgatg tttgcgatga
    10321 tccccattga tgatccttta ttcgtgattg ctcctgcaat tgctccaatg ataccgccga
    10381 cgattaatgc ccaaataaat cccattttaa attcctccta actgacttct tacttcttat
    10441 tgtacacgtg gttcgttatt ttctttgact ttttgtgcac ctttgcctac agcagatttt
    10501 gctttatctg tttggcgtga agcaaattgt cctgtggatt ttgctgcttc tgttactttg
    10561 tcttgtactg tttcttgatc tttttggtat tcttcttgtg ttttgatgtc tgcaacattc
    10621 acattcactt cgataacatc taaatgtgtc atattgttta cttcagtact gatcaattct
    10681 ttgatttggt tgtaaatatc ttcgatgtct ttaccgtatt ctacgatcac atctagatcc
    10741 acagctactt gtttcttacc tacttctgta ttgatacctg ctgttacgtt atctgtgttg
    10801 actaattttt cagccatatt tgagaaaaat ccgccatcta atgtcaatag accatctaca
    10861 tctttcaatg cataaccgat gattttttgg atgactttgt cttcataagt caattcacca
    10921 ttcatcatgc ggttttctcc tttttcattt gccaaaactg atccatttgt tgtattcatg
    10981 tttttgtttt ccatctttgt ttcctcctat gatttaaaaa attgatcaac tattcctgac
    11041 tgtttcaaat aaaaaccgat tcctgcgcca attgctgtca tgattatgat caacaatgtc
    11101 ttgaaaaatc caatcgttaa ccataaaaca gctagcagta gtccaagacc cccaaagata
    11161 atagggagct tgtatgctga aaatagttct ttcattattt ggccctccta ttccactcga
    11221 gagtgcttgt agtctcttgt atcttcttgt tcttcataac cgctgtatgt gactgccact
    11281 ttgaccgttt cttttgttcc gagtacttta gttacttctt cttcgatatc ttgcatcaat
    11341 acacctgttt tccctactaa tgaagaagtt cgtttcaagt ctccgttgac atggatatgg
    11401 attcgatttt tcgttgaacg tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt
    11461 aaatgggaac gtaccaaccc ttcgatcgct tttttgtcga ttgttagttt gccatcttcc
    11521 cgtttcataa cgaaagttcc tcttgacttt ggatagaaaa gaacgactag caagatgata
    11581 atagcaagaa cggcaaatac tacagctgcc cagaaaatat attgttgcat gtagtaattc
    11641 gttaaagtaa agaagcgaaa tcgttccaat tgttcaggca taatatacgg cgcattgatc
    11701 aacgcaatcg ctagaaatac tgaaagtagc agtaaactca tgataacacc gattattttt
    11761 aaccccttat tcattttcca cctcctataa acttattttt accctgtaaa taactctacc
    11821 gacaatcaca gtatagatag cgaacagtta gcatagcatc agtaaataaa gcgctaccaa
    11881 acattgctgg atcataacgg cgttttattg ccagtctgat actgaaataa caattgctta
    11941 cttattctct atttactttt gattgctggc tatacttaaa ggatattatt caagattaaa
    12001 aattgcaaag attatgctca gttattatta caagattcta tttatttatt aaattttttc
    12061 ggagaattaa aaaatacaat aaataacttt agttacttta atgcgttctt actttatttt
    12121 tatattatca tttttcttat tcacttcttt ggtttatact attcttataa ataaaaatga
    12181 ggtatgtcct atgatgaaaa gattgtacta ttcattgatc ataacgattg gctatttgat
    12241 tgtatcaaat ctaggaaata tggtatttgg tatcagtaaa gaattcagtt ggactactac
    12301 attatgggaa tctctattct ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa
    12361 ataaacatgt gccatctatc tgttggcaca tgttttagga tttatggtac tttttattcg
    12421 gcaatccatt ttatttttct acttcttctt aatacctcta acacttttga tttataaact
    12481 aatccaatgc atctacttgc gcttattaag ctatttcagc tagatagtac caccccatgc
    12541 gtcaatgctt gattttcctt ttgtataaca ttgtataagc aataaaggac aaataagtgg
    12601 tgattacacc aaaccattga ataagaaaaa ttctgatacg taaataaaca tgaacttgaa
    12661 aaaattaatg aatcggttag ctactacgta gttgtctttt ttctcataca gttctgttaa
    12721 cttacacttc tcttcgctag ataataaaaa atcctccaac tgtattgtcc aatataatcc
    12781 caggcatcta aactagcatt atagaagaga cacacataga atctaaaata actggtttta
    12841 aatgtttctt acaatcaaaa aaagctgtta aatgcactcc acaccttcat actctatcat
    12901 tttttataaa cgatatattt ttattgtttt tcgataaaaa atagttgttt tacaataatt
    12961 attctgctat attttagcta tcaaagataa aagagggata aaaatgaaat ttaaaacatt
    13021 gctattaaaa tttgtcgttt taggtataag tgccgtattt attttgttcg gcgtactttt
    13081 ttatactcag atcgctacaa gcgaaaaagg attcaatttt gattggcaga ccacattatt
    13141 tgtttctgtc atttactttg cccttctcct agggatcgtt attgcttatt tcttattgaa
    13201 actattgaaa aaaatcgaag ataaccaagc tttttccact caaagcttaa acatactaaa
    13261 aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt tttggcttac ttcctaaatt
    13321 tatgaaagta gcggatttag acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt
    13381 attcattcct tttgcagtat acacattaac aagcgtgctg gaaaaactgc ttgaacatgc
    13441 gattttactt aagacagatc atgatttgac agtgtgaggt gcgacatggc aattatcgtt
    13501 aatttagatg tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg
    13561 ggcattacga tggcaaacat ctctattcta aaaaatggaa aagcaaaagc catccgtttt
    13621 tccactctcg aaaaaatctg ccaagttttg gagtgtcaac caggtgattt gttgacctat
    13681 aaaaatgact cgaatactat accagaagaa taaaataaag tagaacaaac agtaaataaa
    13741 aaataatctg aatgatagat gtgtgagact tttagaagtt atttacgtct attattcaga
    13801 ttttttattt cgattgaata ccgttttatc tataaataag atatgtactg actcatacaa
    13861 agggggcctt ttcttctcca tgtactatca cttttcctga tacaactggt ttcctgttac
    13921 gttgtagttg gatctgcttt tagtataata ctttattcat tccaccagac tgatagcagt
    13981 ttgagcagtc taaataatca tgtagtatta caggccattc tgtttcatac cttgaagtgc
    14041 ttaaaacttc agctaatggc ccattgtgtt aactcttact ccattctttt ttagtaagct
    14101 cccatatata ctgtaaagcc ttctatatat tatgtcatgt tgctttttaa catttactat
    14161 gtagttttga taaaatgatt tgaataacag tgatggacaa ttctttttta tttttaacat
    14221 tattttgata ataatg
//
LOCUS       DACDNO010000067        14149 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDNO010000067 DACDNO010000000
VERSION     DACDNO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.067"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(7..3660)
                     /gene="rpoC"
                     /locus_tag="IWI22_002251"
     CDS             complement(7..3660)
                     /gene="rpoC"
                     /locus_tag="IWI22_002251"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9169623.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3739..7356)
                     /gene="rpoB"
                     /locus_tag="IWI22_002252"
     CDS             complement(3739..7356)
                     /gene="rpoB"
                     /locus_tag="IWI22_002252"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9169624.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8084..9052
                     /locus_tag="IWI22_002253"
     CDS             8084..9052
                     /locus_tag="IWI22_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9169625.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9070..9975
                     /locus_tag="IWI22_002254"
     CDS             9070..9975
                     /locus_tag="IWI22_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9169626.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9972..10778
                     /locus_tag="IWI22_002255"
     CDS             9972..10778
                     /locus_tag="IWI22_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9169627.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10935..11903
                     /locus_tag="IWI22_002256"
     CDS             10935..11903
                     /locus_tag="IWI22_002256"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9169628.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12220..12744)
                     /locus_tag="IWI22_002257"
     CDS             complement(12220..12744)
                     /locus_tag="IWI22_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9169629.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13031..>14149
                     /gene="scm"
                     /locus_tag="IWI22_002258"
     CDS             13031..>14149
                     /gene="scm"
                     /locus_tag="IWI22_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9169630.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDT"
BASE COUNT         4008 a         3057 c         2795 g         4289 t
ORIGIN      
        1 aaatatctat tttgtttgat tgttcattgc gtcttctgca gccatttgcg cttcaatatc
       61 tgaaatactg tagacatttt cgcttgctac accgacttct tttggttcca tgtcacgata
      121 acgttgcata ccagtaccag ctgggatgat cttacctata ataacatttt ctttcaaacc
      181 aagaagagga tctttcttac cgcggatcgc agcatccgtc aagacacgag ttgtttcttg
      241 gaatgatgca gcagataaga aactatttgt ttctaatgat gcttttgtga tacctagtaa
      301 gacaggtcga gaagttgcag gaacgcctcc tgagactagc gtattgtagt tttgatcttt
      361 aaagtctgcg atgtctaata atgtacctgg taagatatct gtatcacctg gatccattac
      421 acgaacttta cgcagcattt gacgaaccat tacttcgata tgcttgtcgc cgatttctac
      481 cccttgcata cggtaaacac gttgtacttc acgaagcaag tagttttcaa cagataatac
      541 atcgcgaact tgcaacaatt gttttggatc gattgatcct tctgtcaatg gtgcaccacg
      601 gtggatcata tcgccttctg ctactttcat acgtgctgta taaggaacag tatacgtacg
      661 tgtatctgtt tttcctttga ttgttacttc tttggtacgt gttgcaggat cttctgagat
      721 atcgatgaca tcccctgtta cttctgtgat cactgcttgc cctttcggat tacgtgcttc
      781 aaagatttct tggacacgag gaagaccttg agtgatatcg tctccggcaa ccccacctgt
      841 atggaatgta cgcatcgtca actgagtacc aggttcaccg attgattgag cggcgatcgt
      901 accaactgct tctccgactt caacgtcaga gccagttgca aggttgcgtc cgtaacaatg
      961 tttgcagaca ccatgttttg tattacatgt gaagacagaa cggattgtca ctgtttcgac
     1021 acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc aattggtttc ctgcaatgat
     1081 caattcattt gtttcaggat gacgtacttc tttacgggtg taacgtccca ctaaacgatc
     1141 ttctaatgat tcgatgattt catttccctc gcggatggct tggatttcaa gacctcggtc
     1201 agtaccgcaa tcttcttcac ggatgattac atcttgggcc acgtcaacca gacgacgagt
     1261 caagtaacct gaatcggcag tcttcaaggc tgtatcggtc atccctttac gagccccgtg
     1321 agttgagata aacatttcca agacggatag tccttcacgg aagttcgaaa tgattggcaa
     1381 ttccatgatt cgaccattag gagcggccat caaaccacgc attccggcaa gctgtgtaaa
     1441 gttggaaatg ttaccacgag ctccagaatc agacatcatg aagattggat ttttcgcttc
     1501 caaaccttcc atcagttttt gttggatgct gtcttttgca gcattccaaa cagcaatgac
     1561 acgttcataa cgttcgtcat ctgtgatcag accacgacgg aattgttttg taatagtttc
     1621 aacttgttta tgggcatttt caatgatttc ttgtttttca ttcagtacac tgatgtcagc
     1681 gatccctact gtgataccgg catgtgtcga atgtttgtac cctaagtctt tcatacggtc
     1741 aagcatctta gaagtttctg ttactttgaa tcgtttgaag acttctgcga tgatgtttcc
     1801 taagtttttc ttcttgaacg gtaatactaa ttcttgctct ttgatatgtg caggaatatc
     1861 agtaccagct tcaacaaagt atttgtccgg tgtttggacg gtcaagttga agtctgttgg
     1921 ttcattcaga taagggaatt caggaggcat gatctcgttg aagatgatct taccaactgt
     1981 tgtgatcatc gtacgttctt tttgccattc agtaaatggt ttttcaccta atgagttagg
     2041 gttcacaccg atacgtgaat gtaaatgaac atatccgttg cgccatgcga taacggcttc
     2101 gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct tcttcttcca ttgtcaagta
     2161 gtagttacct agaaccatat cttgagaagg agtaacaact ggtttaccat ctttagggtt
     2221 caagatgttt tgagcagcta acatcaacat acgtgcttct gcttgtgctt cttcattcaa
     2281 tggaacgtgg acagccattt ggtctccatc gaaatcggca ttataagctt cacatacaag
     2341 tggatgcaaa cggattgcgc gtccttgtac taaaactggt tcgaatgctt ggataccaag
     2401 tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt tccttgatta cgtcttctaa
     2461 aacatcccat acttcgtctt cttgacgttc gattttacgt ttcgcatttt tgatgtttga
     2521 agctagttcg cgttgaacaa gctcacgcat cacgaatggt ttgaataatt caatggccat
     2581 ttcttttgga agtccacatt ggtacatctt caagaaaggt ccaacaacga taacggaacg
     2641 acctgagtag tcgacacgtt tacctagcaa gttttgacgg aaacgacctt gtttcccttt
     2701 caacatatga gaaagagatt tcaatgggcg gttacctggt ccagtaactg gacgtccgcg
     2761 acgaccgtta tcgatcaatg catctactgc ttcttgcagc atacgttttt cattttgaac
     2821 gatgattcca ggtgcattca agtctaacaa acgtttcaaa cggttattac ggttgattac
     2881 acgacggtaa agatcattca agtcacttgt tgcaaaacgt ccaccttcta attgaaccat
     2941 tgggcgtaaa tctggtggaa taacagggat aacgtccata accatccagc ttggttgatt
     3001 tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg attgcacgtg tacgtttttg
     3061 tccttgtgca gatttcaatt cttctttcaa ttcggctgct tctgcatcta gatcaacatt
     3121 atcaagtaat tgtttaatcg cttctgcacc catagcagct tggaattctt gtccatattg
     3181 ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt tttttctcta aattcgtatt
     3241 tcctggttcg atgactacat atgaagcaaa ataaatgatt tcttccaaag cgcgtgggct
     3301 catgtctaat acaagaccca tacgtgaagg aatcccttta aagtaccaga tatgtgaaac
     3361 aggagctgct aattcaatat gtcccatgcg ttcacggcga actttcgaac gagtaacttc
     3421 cactccgcat cgatcacaaa cgattccttt ataacggata cgtttgtatt ttccgcaggc
     3481 acattcccag tcttttgtag gaccaaaaat ccgctcacag aacaaccctt cgcgttcagg
     3541 ttttaaggta cgatagttaa tcgtctcagg tttttttact tcaccgtaag accagcttct
     3601 gattttttcg ggagaagcca aacctatttg catactttcg aatttattta catcgatcaa
     3661 aaggggttcc ctccatttca tttaatggca aaccgcaaga cggctgatct tcgaccgcct
     3721 tgcagctcat agaccggatt agtctaaatt atcttctgcg gtttcaaagt tttggatgac
     3781 atcgtctttt tcatcttcca cttgttcagc agctttcttt tctaattctt tggcagtttg
     3841 ctgttcggca aattttgtca aagcatcaac agtgatcaaa tcgtcgtctt catcgtccat
     3901 gtcgcgaagt tcgatctctg tttcttcgat atccaagaca cgcatgtcca gacctagtga
     3961 ttgcaattct ttcaccaata cgcggaatga ttccggtaca cctggttttg gaattggttc
     4021 acccttgacg attgcttcgt atgttttcac acgtccgact acgtcatctg atttgtatgt
     4081 caagatttct tgtaaagtat aagccgcacc gtaagcttcc agtgcccata cttccatttc
     4141 accaaaacgt tgtccaccaa attgagcttt acctcccaat ggttgttgtg taaccaatga
     4201 gtaaggtccg attgaacgtg catgcaattt gtcatcgacc atgtgagcaa gtttgatcat
     4261 gtacatcaca ccgacagaca cacgtccatc aaatggttca cctgtacgtc cgtcgtacag
     4321 aacagttttc gcatcgctgg ccataccggc ttcacgaact gtttcccata cgtcttcatc
     4381 acttgcacca tcaaatactg gcgttgctac gtggatacct aactgacgtg cagccatacc
     4441 caagtgtaat tccaatactt gcccgatgtt catacgtgaa ggcaccccta aaggattcag
     4501 catgatatcg attggtgttc catcaggtag gaatggcata tcttcttccg gcataatacg
     4561 ggatacaacc cctttatttc cgtgacgacc ggccatttta tccccttcat ggattttacg
     4621 tttttgaacg atataaacac gaactaacat gtttacgcct ggtgacaatt catctccagc
     4681 ttcacgagta aagattttca catcgtgaac gataccgccg ccgccatgtg gtacacgtaa
     4741 tgatgtatcg cggacttcac gagctttttc gccaaagatc gcatgcaata gacgttcttc
     4801 tgcagataat tctgttaccc cttttggcgt gactttccct acaagcaaat cgccatcttg
     4861 gacttctgca ccaatacgga tgatacccat ttcgtctaaa tcttttaatg cgtcttcccc
     4921 gacattaggg atttcgcgtg tgatttcttc aggtcctaat tttgtatcgc gggcttctga
     4981 ttcatattct tcaatatgga tagaagtgta tacgtcatct tttaccaaac gacggctcat
     5041 gatgatcgca tcttcatagt tgtacccttc ccaagtcatg aaaccaacta aaacgttttg
     5101 gcctagagcc atttcgcctt gttccataga tggtccgtct gccagagtat cccctttttc
     5161 gactttctca cccaaatgaa cgattggacg ttggttgtag cttgttccgg agtttgaacg
     5221 acggaatttt gttacatcgt atttatctaa tgcaccgttg tcgcggcgta cgcggatttc
     5281 agaagcatca acgtattcaa cgacaccgtc atgtttacat aacaaagcag ctcctgagtc
     5341 atgcgctgat ttgtattcca taccagtacc aacccatggt gcttgaggat tgatcaacgg
     5401 cactgcttga cgctgcatgt tggcacccat caaggcacgg ttggagtcat cgttttccaa
     5461 gaaaggaata catgctgtcg caacggcaac tacttgttta ggtgatacgt ccatgtagtc
     5521 tactttatca atagatactt ctaagttttc actttgagca cgagccataa caacgtcttg
     5581 agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg gctacgatgt aatggtcttc
     5641 gatatcagct gtcaagtagt cgatttggtc agtaacacga cctgtttcac ggtctacacg
     5701 acgataaggt gtttcgataa atccgaattt atttactttc gcataactgg acaaactatt
     5761 gatcaacccg atgtttgggc cttcaggagt ttcgatcgga cacatacggc catagtggga
     5821 gtagtgaacg tcacgaactt cataaccggc gcggtcacga gtcaatccgc caggtcctaa
     5881 ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg tttgtttgat ccatgaactg
     5941 tgacaactga gaagaaccaa agaattcttt gatacttgcc acaactggac ggatattgat
     6001 caattgttga ggtgtcaatg tttcagtatc ttggatcgac attctttcac gaaccacacg
     6061 ttccatacgt gctagcccga tacggaattg gttttgtaaa agttcaccga ctgaacggat
     6121 acgacgattt cctaagtggt cgatatcatc gacattgccg ataccttcca tcaagttcaa
     6181 gaaatagctc attgaagcaa tgatgtcggc tggacgaacc gtttttacag ctgcttctgg
     6241 atagccgttt ccgatcacgt ttacttcacg ttcagggtct cttggtgaaa atactttgat
     6301 gacttggaca gtcattggat ctgtgaccac accgtcttct gatggatagt aagtcactga
     6361 atttaggcca ttatcaatga aaggagctag tgtttccatg acttggtgag tcagaactgt
     6421 tcctttttca acgatgatct cgcctgtttc tggatcaact aatgtttcag ctaatgtcag
     6481 attcaataga cgtgttttca aatctaattt tttgtttact ttgtaacgac caacgtttgc
     6541 taagtcgtaa cgttttggat cgaagaaacg agcattcagc aagttacgtg agctgtctgc
     6601 tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct ttcaaacctt cttctgttct
     6661 tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta ttgcgtaatg tttcgctgtc
     6721 gccgaagatt tcaaagatcg tatcatcgga accaaatcct aacgcacgaa ctaatacggt
     6781 taaaggaatc ttacgtgtac ggtcgatacg tacataagag atgtcttttg catcagtttc
     6841 catttctagc caagctccac ggttagggat aacagtagaa ccgaatcctt cttttccgtt
     6901 tttgtcgact tttccatgga aatagacacc tggtgaacgg actaactgag aaacgattac
     6961 tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc attaatggga aatcaccgaa
     7021 gaatacttct tgtgctttga tttcgccagt ctcgcggttt gtcaaacgca atgttacatg
     7081 taatggtgca gagtagtttg catcatgcgc acgagcttct gctactgtat actttggttc
     7141 tttcaattca taatctacaa attccaaaga tagattgcca ttgaagtcat cgattggcaa
     7201 aatgtcttca aacatttctc taagtccctc atctaagaac cattggtaag agtctgtttg
     7261 aatttcgatc aaatttggta attccaacac ttcactgatt cttgcgaaac ttctacgttc
     7321 gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc ttcacccctc taaaatgtca
     7381 ttcaaaaaga ctacccgatc aattgttaca ataatatttc gaatattatt tttaggtaac
     7441 aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa aaatagaaca ttctcttgaa
     7501 accttacttc gtgagcttct acttttgttt ccttatatta aagccgaaac ggacaatatt
     7561 tcgtttctgc cttttcttca tcagatagtt tttgcaactt catatactac acttattttc
     7621 tataaaagtc aacacttcct ggcgtttctt ctgctttttc gcaacatttt cttaatgttt
     7681 tcaattttat gttttcaatt atctgacaat tcttaattga cagacctttt tatctcggtt
     7741 atactttaga aaattcattt tgtggaggaa cttctatgcg aaccaacatc aaccaaatcg
     7801 caccaacaca actgaataac ttttacttta gctactttag ataggtttgt attcatcatg
     7861 atggatacaa acgttaaacg acagtttgta tctatgatgg ctaaagaact ttgtattgca
     7921 gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag tgaaaataca aagtaaaagt
     7981 atcaagcatt ctactttttc aatttatttt ccgacttttc aatccatttg gacattttgt
     8041 ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa gaaatgaaaa aaaatataat
     8101 catcggatta ggcgctgccc tgctactagc tttaggaggt tgcgcacaaa aagacaattc
     8161 cgcagaggca tcagatggac aaaaaacaat tggcgtattg caacttgtac aacataactc
     8221 gttagatgct gcctataaag gattcaaaga aggattagca gaagaagggt ataaagaagg
     8281 cgataatcta acaatcgatt atcaaaatgc ccaaaacaat caagacaacc tgaaaagtat
     8341 gagtgagaaa ctcgtcaaag aagatcctga tttgctactt ggaatcgcca ctccagcagc
     8401 acaatcttta ttaaatgaaa caaccactat cccgatgact gtcacagcgg tcacagactt
     8461 ggaacaagcc catttagtca catccaatga aaaacctggc ggcaatgtga ctggcacgac
     8521 agatatggtg ccaatcgaaa aacaaatcgg cttactgtta aatatcgtgc cagacgccaa
     8581 aacaatcggc atcatgtata acaacggtga agcaaactct aagatccaag ctgatttggc
     8641 agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta ctgacagcaa attccacaaa
     8701 tgatgtccaa caagtgacga aaagtctagc taaagatgta gatggaatct atattcctac
     8761 tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa gtcgcaaaag aaacaaaaac
     8821 acctgtcgtt gctggcgcag ttgatcaagt caaagacggc ggtctagcaa cagtaggaat
     8881 cgactacgaa agcttgggca aacaaacagg gatcatggca gcaaaaatct tagacggcaa
     8941 gaatccaagt gaacttcctg tcgaaacagc gaatgatttg gaactgtatg tcaataaaga
     9001 catggccaaa gcattaggca tcaatccaga cagcatcaaa gaagctgaat aaaacacagg
     9061 aggattctca tgacaaattt gctctctctt ttcttatcat ctgcatcgca aggggtctta
     9121 tgggccctac ttgcgattgg tgtatacgtc actttccgta tcttggacat tgccgacttg
     9181 actgctgaag gaagctttcc tttaggtgca gggatcgcgg cagtaagtat tacaaatggc
     9241 tatcatccat tgacagcttg tcttctaggc tttttagggg gtgcggcagc agggcttgtt
     9301 tccggactgc ttcacacaaa attgaaaatt cccgcccttc ttgcagggat catcaccatg
     9361 actggtttat attctgtcac atcccgtgtg atgggtgcgc cgaacatctc tttattgggt
     9421 cagccaacga tctttcattg ggtcgagtca ttcggcataa caaaagacaa cgctgtactg
     9481 atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac ttgttttgtt cttacgtaca
     9541 gaaacaggac ttgctattcg tgcaactggc gataatctgg caatgagcga agcaaacggg
     9601 atcaacacag ataatatgaa gatcatcggt tatatgattt ctaatggatg cattgctctt
     9661 tctggctcct tacttgcgca aaacaacggc tttgctgatt taaattctgg tattggaacg
     9721 atcgtaattg gacttgcttc tattatcatc gcagaagttt tgttacgtaa ccagccacta
     9781 tttctccgtc tcttgactgt cgttttaggt gcggtcattt atcgttttat tttagcgtta
     9841 gtcttcgaac tgaatgttga accaagtgat tcgaaacttg cttctgcttt cgtgctagtg
     9901 atctgtcttt ctttcccgca gatccagcaa aaattccatt tacctcaaaa aaagaaaggc
     9961 ggtagtgtca aatgaatgac gtcttaacat tggaaaatct ccatcatacc tttgaaaaag
    10021 gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct ttccttagat cccggcgact
    10081 tcgtgacgat catcggtgga aatggggctg gtaaatctac tttgctgaac agcatcgccg
    10141 gtacattttc tgtcgatcag ggtcaaattt tattgaatgg aaaaaatatc acgaaaaaat
    10201 ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca agatcctaaa ctagggacag
    10261 ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat gaaacgagga aaaaaacgcg
    10321 gcttcttccg tggtgtcaaa ccacaagacc gaacattttt caaagaccag ctggttcggc
    10381 taaatttggg gttggaaaat cgtttatcct cagaaatcgg tttgctgtct ggaggacaac
    10441 gtcaagctat tacgttattg atggccacat tacagcgtcc agaactgatc ttattagatg
    10501 aacacactgc agcgcttgac cctgctactt ccaaaacggt gatggaattg acagaaaagc
    10561 tgatcactga gcagaatctc actgctttta tggtcactca tgacatggaa gacgcgatcc
    10621 gctatgggag tcgactgatc atgctgcatc aaggaaggat cgtcgtagat atccagcaag
    10681 aggaaaaaca gcagctcaca gtgccccaat tattagcgtt gttcaaacaa aacagcggca
    10741 cggcattaaa agacgatcaa gtacttttat cttcataatc agaaagagga tgggacaaaa
    10801 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
    10861 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
    10921 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
    10981 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
    11041 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
    11101 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
    11161 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
    11221 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
    11281 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
    11341 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
    11401 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
    11461 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
    11521 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
    11581 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
    11641 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
    11701 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
    11761 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
    11821 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
    11881 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
    11941 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
    12001 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
    12061 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
    12121 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
    12181 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
    12241 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
    12301 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
    12361 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
    12421 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
    12481 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
    12541 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
    12601 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
    12661 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
    12721 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
    12781 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
    12841 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
    12901 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
    12961 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
    13021 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
    13081 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
    13141 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
    13201 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
    13261 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
    13321 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
    13381 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
    13441 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
    13501 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
    13561 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
    13621 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
    13681 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
    13741 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
    13801 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
    13861 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
    13921 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
    13981 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
    14041 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catcaacttc
    14101 aagcgacagt acagatacta cctctaccac aagtgatagc acggataca
//
LOCUS       DACDNO010000068        13974 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDNO010000068 DACDNO010000000
VERSION     DACDNO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.068"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            162..803
                     /locus_tag="IWI22_002259"
     CDS             162..803
                     /locus_tag="IWI22_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9169631.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            834..1538
                     /locus_tag="IWI22_002260"
     CDS             834..1538
                     /locus_tag="IWI22_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9169632.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMEQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQDFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            1535..2437
                     /locus_tag="IWI22_002261"
     CDS             1535..2437
                     /locus_tag="IWI22_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9169633.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQLG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     LDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2595..4301)
                     /locus_tag="IWI22_002262"
     CDS             complement(2595..4301)
                     /locus_tag="IWI22_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9169634.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIAELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFHRLSQTELEPKAIQRQFQGIGFDTAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKNFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRDGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTEYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            4491..5114
                     /locus_tag="IWI22_002263"
     CDS             4491..5114
                     /locus_tag="IWI22_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169635.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIRQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            5564..6613
                     /locus_tag="IWI22_002264"
     CDS             5564..6613
                     /locus_tag="IWI22_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9169636.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDVLIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVA
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            6610..7491
                     /locus_tag="IWI22_002265"
     CDS             6610..7491
                     /locus_tag="IWI22_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9169637.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            7494..8264
                     /locus_tag="IWI22_002266"
     CDS             7494..8264
                     /locus_tag="IWI22_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9169638.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCKEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            8543..9397
                     /gene="aroE"
                     /locus_tag="IWI22_002267"
     CDS             8543..9397
                     /gene="aroE"
                     /locus_tag="IWI22_002267"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9169639.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            9575..10738
                     /locus_tag="IWI22_002268"
     CDS             9575..10738
                     /locus_tag="IWI22_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9169640.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            10855..11265
                     /locus_tag="IWI22_002269"
     CDS             10855..11265
                     /locus_tag="IWI22_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9169641.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            11439..13667
                     /gene="topB"
                     /locus_tag="IWI22_002270"
     CDS             11439..13667
                     /gene="topB"
                     /locus_tag="IWI22_002270"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9169642.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         4667 a         2379 c         3005 g         3923 t
ORIGIN      
        1 gaagagaata atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa
       61 gggagctgtt tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat
      121 atgatattcg gaaaaagaaa tctcaaaggg aggtaacttt tatgggattg aatgaattta
      181 ttttaggatt gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag
      241 agcatacagt tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca
      301 ttgaagaagc gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc
      361 atgactacac agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag
      421 agttaagagg gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag
      481 agattccagc tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa
      541 ccgtagaagg agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg
      601 atgaaatcat caaaaataac ccggaaaaag tttataagga gatgtttatt gaggcagtga
      661 ctacgattaa aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc
      721 cggaactatt gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga
      781 ttgtaaagaa agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa
      841 agaatatgga tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt
      901 tatgtgattg gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca
      961 agggggaaga aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt
     1021 ttactgacaa ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg
     1081 tagagttcaa gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac
     1141 atatcatcaa cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa
     1201 aagagaatct agtcgtgggt gtcatgtacc agctaatgga acaggaaggt tggctgtata
     1261 atcaagaaaa tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg
     1321 tgttctttcc ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta
     1381 ccagcgggcc aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagattttc
     1441 ttctcccctt cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg
     1501 gaagagaaat ttattatttc gaggagtttg agcaatgaat atccagcaga tgaaatacgt
     1561 ggtcgctgta gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca
     1621 gccaagtctt tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac
     1681 tcgaacgaat cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga
     1741 gaaaatattg gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga
     1801 caatttttcg atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact
     1861 cgaggaattc aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt
     1921 gatcgaagaa gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa
     1981 tcaagctggt atcagaagat atttacaact aggagagcta agtggccgca gcattggaaa
     2041 attcaaaacg catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac
     2101 caaagaagaa ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc
     2161 gtatttttat gaagacctta tcgagatcct tgatcaagaa acagtgattt atacgagtga
     2221 tcgaggtact ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat
     2281 tgtgattgga gggattcgcg agcatttgcg gttaatccct ttggcagaag gcgaactaaa
     2341 tgaattttat atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat
     2401 caaagagctt actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt
     2461 tattgaacac cgttaatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt
     2521 tttgtagcaa acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta
     2581 gaaacactca ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc
     2641 acgataatcg tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt
     2701 cggatatgct ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa
     2761 aaataggcag ctaactcggc agcttctgta attgtttcat cggagggctt gtcactttta
     2821 atgatgacgt gcgaaccggg tatatttttt gcatgaagcc aatattcggt ttttttagct
     2881 gtcttcattg tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg
     2941 tcagaagaaa agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc
     3001 ttctttttca ggtatccttc tgaagtcaat tcttctttga ttgcttcgat atccattggg
     3061 ccggcaattt ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct
     3121 tggatttgtt cgccaatcag cttaacagcg ttcttcagct tttggtaacg atggaaatat
     3181 ttctgtgcat tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct
     3241 tcttcataat aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa
     3301 gtagtaagta gttcgccatc tctacggtaa ttctcagcgt tttcggattc tttgagtgtt
     3361 tgttcacgtt ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc
     3421 agttctccgc cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct
     3481 aataattgac tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga
     3541 taagcaatcg gtgtgaaaaa atttttattt tccgtttcgt aaaaagtagg aataggctga
     3601 tggctgatgg ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt
     3661 aatcttttgg tcagctcttg agcggtatcg aaaccgattc cctgaaactg acgttggatt
     3721 gcttttggct ctagttcagt ttgggaaagc cgatgaaaga ttttttcttt ctcggagcta
     3781 aatgggttca agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa
     3841 cgataagtgt tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct
     3901 cgattaacta agacgatcgt actatgacgt cccattaatt cagcgatcaa tatgatattt
     3961 tgtagatcac ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg
     4021 atttgttcga tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag
     4081 ttaggcggat tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt
     4141 gggtgcgcag ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt
     4201 tcattttcat aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat
     4261 tcgttgacca tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt
     4321 ttcataagta tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa
     4381 agtaaaagtc tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa
     4441 atccaaattt ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta
     4501 tttgggaagt agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg
     4561 cagaaaatcc aagtaagtat tttcgacgtc ttgtgtttct gctcatctta tcattgctgt
     4621 taacagttgc aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg
     4681 tttattcgtc cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga
     4741 aaatcagaca aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc
     4801 aggacatgct tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct
     4861 tttattatcc gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg
     4921 atgaagtgac atttattttt tttgatggag gaaaagaagc aaatcggata actgcgggta
     4981 agggaggaat aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag
     5041 ccaatatcaa acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg
     5101 agattgctaa ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa
     5161 ttatcagaac aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt
     5221 tgacgaaatt caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta
     5281 atgaacttat caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga
     5341 taagttgtat taatggtgag actttcccat agatacaact ttttagagtg catttctaaa
     5401 gcttgtatct atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt
     5461 tttgtgtaaa gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc
     5521 taattataat aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt
     5581 aataacagtt gtagtaatta ttttagttta tttagcaggc gcatttattt atggaaaagc
     5641 ctcaggcgaa aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt
     5701 attgcaatac gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct
     5761 gaaagaagaa ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc
     5821 cgatcagagc aagctagcca caatgagtca acagctagta caatctgatc cagatgtctt
     5881 gattggaatc gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt
     5941 tgtgttaggt gctgtaacgg atcctgtggg ggctggatta gttgcttctt tagagaaacc
     6001 tggtggtaat gtaacaggtg tttctgatca gccgcctgtt gcttcacaaa tcaagcttgc
     6061 ttctgatttg ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa
     6121 ttcaaaatac caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt
     6181 aaaatatgcg gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca
     6241 agtcgatttt atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt
     6301 aaaagaagca aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca
     6361 aggtggacta gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat
     6421 ggcagcagaa cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga
     6481 tcaaggggat acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca
     6541 atcgatgaag gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga
     6601 ggatgacaaa tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta
     6661 gggattttca tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc
     6721 ccattaggtg ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct
     6781 acattattag gagtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca
     6841 aaaggaaaga taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt
     6901 attttgtttg tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac
     6961 agcttccatc gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct
     7021 ttgggcatct tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat
     7081 attgcgacag gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg
     7141 aaaattttag gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc
     7201 cagaacgacg gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct
     7261 tccatcatca tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct
     7321 attgttgtcg gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt
     7381 gacacgactt atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg
     7441 caattgaaga aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg
     7501 atatattgac aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag
     7561 tactattgga tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg
     7621 ggaatggtgc tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc
     7681 aaggatcgat ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac
     7741 gattcctttc tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag
     7801 cagaaaattt gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact
     7861 tgaaaaatca aaaagaagat tttttcagac tatgtaaaga agtcggtaat ggcctagagc
     7921 aacacttgga tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc
     7981 tgatggcgac gatcacagct cctgagttac tattattaga tgaacatact gcagctttag
     8041 atcctaaaac agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat
     8101 tgacttgtct catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga
     8161 tcgtactcca aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga
     8221 caatgggcga ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt
     8281 cctttcagta gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta
     8341 aaacaataaa caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta
     8401 acaattaaat agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact
     8461 ttgccaccta gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa
     8521 gtaagaacaa aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc
     8581 tgatccggct tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa
     8641 catcgatgcg gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga
     8701 aagtattcga acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt
     8761 tattccttat ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat
     8821 cgtgcacgaa aagggtcgtt tgattggcta taataccgat ggtgcgggct tcatgcaatc
     8881 cgtgcaagaa gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg
     8941 tgcagcaaag gcagtcactg ttcaagctgc gttagacggc gctgaggaaa tcgtagttta
     9001 taaacgaaac aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg
     9061 aacttcttgt aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt
     9121 gaaagaaagc tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca
     9181 gtcaccgctc ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta
     9241 tgcacctaga gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa
     9301 tggacttggc atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa
     9361 gatgccgatc catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct
     9421 tttattctcg cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt
     9481 cgttattttt tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc
     9541 tttatttatc agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga
     9601 catccataag aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga
     9661 acgaaaacga acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca
     9721 gcattcattt gaacatttga accaatacga gcaacagata tacagagatg caccaagagc
     9781 agaagaacaa caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa
     9841 acctcgaaga aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct
     9901 ttattcaggt atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga
     9961 ggcagcgact tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt
    10021 gatccttgga agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt
    10081 acagttggat ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt
    10141 tacgatccca ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga
    10201 tctagtgcgt caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt
    10261 cgatttcaaa tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga
    10321 tgcagaaaaa gatctgaatc tggatggtgt agatattaaa aaaggggtcc agaagatgga
    10381 tggacatgtt cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg
    10441 tgtcagacgc caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa
    10501 tctgatccgt gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac
    10561 tgcttggttc ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg
    10621 aataagtgtg ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat
    10681 tcttgaaatt gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc
    10741 ataaaaaaga tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca
    10801 ctcgtaaaaa acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat
    10861 atctatctga acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt
    10921 cagactgaag catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac
    10981 ttgccagaag aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta
    11041 gttatgtttg cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt
    11101 tctttagtca tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct
    11161 gagggtggta agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg
    11221 gtaactgatc gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat
    11281 attttctgga aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt
    11341 ctatttcgac tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata
    11401 gagaagtccg caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg
    11461 aaaaaccttc acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag
    11521 ggtattatga aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg
    11581 gacatttggt agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt
    11641 tagaaaattt gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg
    11701 cacaattttc gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag
    11761 atggtgatcg agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt
    11821 ctaaggaaaa gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg
    11881 aaggttttaa gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag
    11941 caaggcagaa cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac
    12001 ttcaacgaaa agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct
    12061 atatgattta tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag
    12121 gagaaggttt aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag
    12181 catttaaaac ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta
    12241 agcaaaacgg acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt
    12301 tattttcgct atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag
    12361 ctacacttga tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg
    12421 attcctttta cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata
    12481 aaaaattttt aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg
    12541 tcgatgcgaa aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc
    12601 ctgcacgctt tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca
    12661 ctactgtggc tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg
    12721 tacagcaatt aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa
    12781 tcttacgcat caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg
    12841 aagaagcaga agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata
    12901 ccgaaggaac gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag
    12961 cgcaagaaat tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca
    13021 ttgaaaatct taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc
    13081 caaaaggaat cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa
    13141 tgactgcgcg ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg
    13201 tttttttaga gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa
    13261 tcgacaaggt agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga
    13321 agaaaaacga tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc
    13381 ctaaaatcgc tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta
    13441 aaaagaagct gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag
    13501 ttgtcaaagg ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag
    13561 atatgacaat cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg
    13621 gaaacaaaga aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga
    13681 aatagcggaa tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat
    13741 cagtgaatca agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt
    13801 ttatttgaaa aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc
    13861 tgtgtaccaa gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca
    13921 caatcaaaca gcatattcac caacgttgat tttatcgact ttgactgcag gagc
//
LOCUS       DACDNO010000069        13818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDNO010000069 DACDNO010000000
VERSION     DACDNO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.069"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWI22_002271"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="IWI22_002271"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9169643.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="IWI22_002272"
     CDS             complement(2262..2513)
                     /locus_tag="IWI22_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9169644.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="IWI22_002273"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="IWI22_002273"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9169645.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="IWI22_002274"
     CDS             3463..3681
                     /locus_tag="IWI22_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169646.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="IWI22_002275"
     CDS             complement(3970..4482)
                     /locus_tag="IWI22_002275"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9169647.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IWI22_002276"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="IWI22_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9169648.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7369)
                     /locus_tag="IWI22_002277"
     CDS             complement(6935..7369)
                     /locus_tag="IWI22_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9169649.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="IWI22_002278"
     CDS             complement(7379..8167)
                     /locus_tag="IWI22_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9169650.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="IWI22_002279"
     CDS             complement(8164..9105)
                     /locus_tag="IWI22_002279"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9169651.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="IWI22_002280"
     CDS             complement(9282..9869)
                     /locus_tag="IWI22_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9169652.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="IWI22_002281"
     CDS             complement(10090..11403)
                     /gene="obgE"
                     /locus_tag="IWI22_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9169653.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11700..12710
                     /gene="gap"
                     /locus_tag="IWI22_002282"
     CDS             11700..12710
                     /gene="gap"
                     /locus_tag="IWI22_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9169654.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12795..13730)
                     /locus_tag="IWI22_002283"
     CDS             complement(12795..13730)
                     /locus_tag="IWI22_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9169655.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3810 a         3096 c         2436 g         4476 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgta aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgtatta attgaggatg ccgtttcaca gcacccctaa taaaggagga acttttgata
    11701 tgacagtaaa agtaggtatt aacggatttg ggcgtattgg ccgtctggct ttccgccgca
    11761 tcaaagaagt ctcagatgat attgaagtag tagcaatcaa cgacttaaca agtccaacta
    11821 tgttagcaca attattgcaa tttgactcta ctcatggtac ttatcctggc acagttactg
    11881 caacagaaga ctcaatcgta gtggatggcg aagcaactcg tgtttatgca gaacccgacg
    11941 caagcaaaat cccttgggca aaagaaaacg gtgtagatat cgtactagaa tgtactggtt
    12001 tttatacatc tgaagaaaaa gcaaaagcgc atttagatgc tggtgtcaaa cgagtagtta
    12061 tttctgcccc agctggtgct atgaaaacca tcgtctataa cgtcaatgat gatacattag
    12121 atgaaaacga caaaatcatc tcagcaggtt catgtacaac aaactgttta gcacctatgg
    12181 cttatttctt aaataatgaa tttgggatcg aagttggtac aatgacaaca gttcatgcct
    12241 acacttccac acaaatgctc ttagatggac ctgtaaaagg cggtaatttg cgtgctgctc
    12301 gttcagctgc tgacaatact atcccacatt ctactggtgc tgcgaaagca atcggcttag
    12361 ttatccctga gctacaagga aaattacaag gacacgcaca acgtgttcca gtcgttgatg
    12421 gttcattaac tgaattggtt tcagtattga aaacaaaagt aactgctgat caagtaaacg
    12481 aagcaatcaa aaaacacaca gttgacaatc cttcattcgg gtatgatgac cgtcaaatcg
    12541 tttctagcga tgtcattgga acaactcaag gttcgatctt tgacccaacg caaacagaag
    12601 taacaactgc tggtgatttc caacttgtta aaactgttgc atggtatgac aatgagtatg
    12661 gatttacttg ccaaatgatt cgcttactag aaaaattcgc taacttgtaa tttgatagat
    12721 caatcgataa aagaagcctt tgactggatc cagtcaaagg cttctttctt ttcatcccct
    12781 acttttaaac tcgttcaatg aacatattgg cttaaaaatt gttggactac ttgctgatat
    12841 ttttcaggat ctgtcttgta tgcttccgca tgctcggctc cttttacaac atacttttct
    12901 tttggtccct tcgttgcacg atagacctta tctaacattt caaatggtac aaatgtatct
    12961 tcgtctccat gtataaagag cattgggcgc tggtttttct tcaattgttc cactgcattt
    13021 gcttcaccga aaaagtagcc agcacggact tttgtcacta gactcgttac ttgcataagc
    13081 ggaaatgctg gaagactaaa catatcgtct agctgataag caagctcatc atgaacggaa
    13141 gaataaccgc aatcttcaat gattgctttc acattatctg gtagtttttc tccgctagtc
    13201 atcataactg ttgctgctcc catgctgaca ccgtatagaa cgatttcttg cgattttcca
    13261 ttgttttcta gcactttgtt tatccactgt acatagtctt tacgctctgg ccatccaaat
    13321 ccaatgtaat ccccttcgct ttttccatgt cctcttgcat caggaacaag gacattgtaa
    13381 cccatatcat gaaacatctt tgcgtaattg gtcatcgttt cagcgtttcc catatagcca
    13441 tgagccacaa gagctgtttt ttcacttttc gtttcagcag gaagataaat cgctgataat
    13501 ttcagaccgt cttttgaagt gatggtccaa tcgcttcggt tttcttttga actaaaccat
    13561 ttatgtgcat ccgtttcttt ggatgtctgt gttgtaccag gtgtatcttc gtctaaaaag
    13621 tctttctcag aaggcacaac agcataatta taaaaataat tccctgcaaa cactaacgca
    13681 acaatcagag ctaaagcgac gcctaaaagg attttcatcc aaagtttcat aaatcatcca
    13741 tctcatttct cttttttcat ataaccttga attagtataa tcaaagaatc tctactatgc
    13801 aatgttcatt tttataac
//
LOCUS       DACDNO010000070        13733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDNO010000070 DACDNO010000000
VERSION     DACDNO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.070"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(63..1268)
                     /locus_tag="IWI22_002284"
     CDS             complement(63..1268)
                     /locus_tag="IWI22_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9169656.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IWI22_002285"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IWI22_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IWI22_002286"
     CDS             complement(1447..2832)
                     /locus_tag="IWI22_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9169657.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IWI22_002287"
     CDS             complement(2861..3247)
                     /locus_tag="IWI22_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9169658.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IWI22_002288"
     CDS             complement(3263..3577)
                     /locus_tag="IWI22_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9169659.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IWI22_002289"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IWI22_002289"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9169660.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IWI22_002290"
     CDS             4837..6126
                     /locus_tag="IWI22_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9169661.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="IWI22_002291"
     CDS             complement(6165..6770)
                     /locus_tag="IWI22_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9169662.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="IWI22_002292"
     CDS             complement(6966..7430)
                     /locus_tag="IWI22_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9169663.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="IWI22_002293"
     CDS             complement(7452..7772)
                     /locus_tag="IWI22_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9169664.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IWI22_002294"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IWI22_002294"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9169665.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="IWI22_002295"
     CDS             9194..9544
                     /locus_tag="IWI22_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9169666.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="IWI22_002296"
     CDS             9729..10682
                     /locus_tag="IWI22_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9169667.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="IWI22_002297"
     CDS             10695..12482
                     /locus_tag="IWI22_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169668.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="IWI22_002298"
     CDS             12469..13671
                     /locus_tag="IWI22_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169669.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3938 a         2689 c         2520 g         4586 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg gag
//
LOCUS       DACDNO010000071        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDNO010000071 DACDNO010000000
VERSION     DACDNO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.071"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWI22_002299"
     CDS             complement(<1..914)
                     /locus_tag="IWI22_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9169670.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWI22_002300"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWI22_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9169671.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWI22_002301"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWI22_002301"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9169672.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWI22_002302"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWI22_002302"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9169673.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWI22_002303"
     CDS             4440..5321
                     /locus_tag="IWI22_002303"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9169674.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWI22_002304"
     CDS             5318..6034
                     /locus_tag="IWI22_002304"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9169675.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWI22_002305"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWI22_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9169676.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWI22_002306"
     CDS             6751..7752
                     /locus_tag="IWI22_002306"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9169677.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWI22_002307"
     CDS             7749..9110
                     /locus_tag="IWI22_002307"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9169678.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWI22_002308"
     CDS             9070..10221
                     /locus_tag="IWI22_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9169679.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWI22_002309"
     CDS             10196..11365
                     /locus_tag="IWI22_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9169680.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWI22_002310"
     CDS             11540..12919
                     /locus_tag="IWI22_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9169681.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWI22_002311"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWI22_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4486 a         2340 c         2922 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatggttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatatgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caattcttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa accagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcaaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDNO010000072        13335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDNO010000072 DACDNO010000000
VERSION     DACDNO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.072"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(178..735)
                     /gene="frr"
                     /locus_tag="IWI22_002312"
     CDS             complement(178..735)
                     /gene="frr"
                     /locus_tag="IWI22_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9169682.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(738..1460)
                     /locus_tag="IWI22_002313"
     CDS             complement(738..1460)
                     /locus_tag="IWI22_002313"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9169683.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1596..2477)
                     /locus_tag="IWI22_002314"
     CDS             complement(1596..2477)
                     /locus_tag="IWI22_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9169684.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IWI22_002315"
     CDS             complement(2576..3358)
                     /gene="rpsB"
                     /locus_tag="IWI22_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9169685.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3717..4196
                     /locus_tag="IWI22_002316"
     CDS             3717..4196
                     /locus_tag="IWI22_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9169686.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4413..5504
                     /gene="phnW"
                     /locus_tag="IWI22_002317"
     CDS             4413..5504
                     /gene="phnW"
                     /locus_tag="IWI22_002317"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9169687.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5497..5625
                     /locus_tag="IWI22_002318"
     CDS             5497..5625
                     /locus_tag="IWI22_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9169688.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5629..6174
                     /locus_tag="IWI22_002319"
     CDS             5629..6174
                     /locus_tag="IWI22_002319"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9169689.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6628..8319)
                     /locus_tag="IWI22_002320"
     CDS             complement(6628..8319)
                     /locus_tag="IWI22_002320"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9169690.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IWI22_002321"
     CDS             complement(8739..9686)
                     /gene="arcC"
                     /locus_tag="IWI22_002321"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9169691.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IWI22_002322"
     CDS             complement(9801..10820)
                     /gene="argF"
                     /locus_tag="IWI22_002322"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9169692.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IWI22_002323"
     CDS             complement(10911..12140)
                     /gene="arcA"
                     /locus_tag="IWI22_002323"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9169693.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12601..13302
                     /locus_tag="IWI22_002324"
     CDS             12601..13302
                     /locus_tag="IWI22_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9169694.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3752 a         2710 c         2368 g         4505 t
ORIGIN      
        1 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
       61 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
      121 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
      181 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
      241 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
      301 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
      361 ttttgcattt tctgcttctt ttttcacatc tttagctaat tcttttctgc gttcttctgt
      421 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
      481 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
      541 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
      601 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
      661 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
      721 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
      781 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
      841 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
      901 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
      961 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
     1021 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
     1081 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
     1141 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
     1201 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
     1261 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
     1321 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
     1381 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
     1441 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
     1501 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
     1561 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
     1621 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
     1681 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
     1741 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
     1801 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
     1861 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
     1921 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
     1981 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
     2041 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
     2101 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
     2161 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
     2221 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
     2281 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
     2341 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
     2401 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
     2461 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
     2521 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
     2581 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
     2641 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
     2701 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
     2761 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
     2821 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
     2881 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
     2941 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
     3001 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
     3061 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
     3121 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
     3181 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
     3241 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
     3301 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
     3361 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
     3421 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
     3481 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
     3541 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
     3601 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
     3661 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
     3721 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
     3781 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
     3841 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
     3901 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
     3961 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
     4021 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
     4081 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
     4141 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
     4201 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
     4261 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
     4321 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
     4381 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
     4441 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
     4501 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
     4561 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
     4621 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
     4681 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
     4741 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
     4801 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
     4861 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
     4921 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
     4981 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
     5041 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
     5101 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
     5161 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
     5221 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
     5281 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
     5341 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
     5401 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
     5461 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
     5521 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
     5581 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
     5641 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
     5701 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
     5761 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
     5821 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
     5881 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
     5941 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
     6001 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
     6061 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
     6121 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
     6181 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
     6241 taacagaata cattttgtac gctgacaaga atccagaatg acttttatac aaaataagag
     6301 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
     6361 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
     6421 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
     6481 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
     6541 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
     6601 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
     6661 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
     6721 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
     6781 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
     6841 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
     6901 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
     6961 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
     7021 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
     7081 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
     7141 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
     7201 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
     7261 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
     7321 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
     7381 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
     7441 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
     7501 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
     7561 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
     7621 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
     7681 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
     7741 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
     7801 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
     7861 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
     7921 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
     7981 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta cggtttgcaa
     8041 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
     8101 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
     8161 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
     8221 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
     8281 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
     8341 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
     8401 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
     8461 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
     8521 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
     8581 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
     8641 tagtcgtttc ataaaaaaaa aatccagatg gcctaaagga gaagaaatac cacctggata
     8701 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
     8761 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
     8821 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
     8881 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
     8941 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
     9001 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
     9061 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
     9121 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
     9181 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
     9241 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
     9301 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
     9361 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
     9421 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
     9481 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
     9541 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
     9601 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
     9661 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
     9721 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
     9781 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
     9841 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
     9901 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
     9961 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    10021 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    10081 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    10141 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    10201 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    10261 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    10321 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    10381 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    10441 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    10501 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    10561 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    10621 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    10681 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    10741 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    10801 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    10861 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    10921 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    10981 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    11041 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    11101 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    11161 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    11221 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    11281 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    11341 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    11401 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    11461 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    11521 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    11581 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    11641 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    11701 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    11761 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    11821 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    11881 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    11941 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    12001 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    12061 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    12121 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    12181 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    12241 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    12301 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    12361 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    12421 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    12481 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    12541 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    12601 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    12661 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    12721 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    12781 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    12841 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    12901 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    12961 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    13021 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    13081 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    13141 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    13201 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    13261 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    13321 gtgaccctga taaat
//
LOCUS       DACDNO010000073        13146 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDNO010000073 DACDNO010000000
VERSION     DACDNO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.073"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IWI22_002325"
     tRNA            complement(77..149)
                     /locus_tag="IWI22_002325"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IWI22_002326"
     CDS             530..1099
                     /locus_tag="IWI22_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9169695.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IWI22_002327"
     CDS             1086..1946
                     /locus_tag="IWI22_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9169696.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IWI22_002328"
     CDS             2136..2840
                     /locus_tag="IWI22_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9169697.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IWI22_002329"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IWI22_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9169698.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IWI22_002330"
     CDS             4677..5993
                     /locus_tag="IWI22_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169699.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IWI22_002331"
     CDS             5994..6863
                     /locus_tag="IWI22_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169700.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IWI22_002332"
     CDS             6918..7727
                     /locus_tag="IWI22_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9169701.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IWI22_002333"
     CDS             complement(7830..8459)
                     /locus_tag="IWI22_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP9169702.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IWI22_002334"
     CDS             8629..9921
                     /locus_tag="IWI22_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9169703.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IWI22_002335"
     CDS             complement(9997..10659)
                     /locus_tag="IWI22_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9169704.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IWI22_002336"
     CDS             10884..11168
                     /locus_tag="IWI22_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9169705.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IWI22_002337"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IWI22_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9169706.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DACDNO010000074        11667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDNO010000074 DACDNO010000000
VERSION     DACDNO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.074"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(423..2432)
                     /gene="metG"
                     /locus_tag="IWI22_002338"
     CDS             complement(423..2432)
                     /gene="metG"
                     /locus_tag="IWI22_002338"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9169707.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2700..2978
                     /locus_tag="IWI22_002339"
     CDS             2700..2978
                     /locus_tag="IWI22_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169708.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            3075..3896
                     /locus_tag="IWI22_002340"
     CDS             3075..3896
                     /locus_tag="IWI22_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9169709.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4181..5191)
                     /locus_tag="IWI22_002341"
     CDS             complement(4181..5191)
                     /locus_tag="IWI22_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9169710.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5203..6156)
                     /locus_tag="IWI22_002342"
     CDS             complement(5203..6156)
                     /locus_tag="IWI22_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9169711.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6146..7216)
                     /locus_tag="IWI22_002343"
     CDS             complement(6146..7216)
                     /locus_tag="IWI22_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169712.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7222..8265)
                     /locus_tag="IWI22_002344"
     CDS             complement(7222..8265)
                     /locus_tag="IWI22_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9169713.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8266..9207)
                     /locus_tag="IWI22_002345"
     CDS             complement(8266..9207)
                     /locus_tag="IWI22_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9169714.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9291..9638
                     /locus_tag="IWI22_002346"
     CDS             9291..9638
                     /locus_tag="IWI22_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9169715.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9708..11369)
                     /locus_tag="IWI22_002347"
     CDS             complement(9708..11369)
                     /locus_tag="IWI22_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9169716.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3322 a         2352 c         2104 g         3889 t
ORIGIN      
        1 ttttatctat attttcaatc acacatataa gagcacaaac taattgaaca taaagtgata
       61 gaacttataa ttaacacatc aacgctactg ttagatataa atatagatgt aataaagtca
      121 ttgcattcaa actttatgaa tgtaaggaca ataaaaaaga gtaccagatg aatcaattaa
      181 aatctgcccg accctttatc attatgcttt ttttctgttt ttacgattcc tattagtatt
      241 attctaaatt aacaaaaatc agcgaagctc gtacttcttt ttctaggatt tattttggat
      301 agttctctgt ttctgtatta tatgattggc ttcaagtaat aagaaggaat ttacgaaaat
      361 tgcttctcaa atttcggaaa aagggggtat gacacaacta gtgtcatacc ccctttgaat
      421 ttctatgcga tttctgagcc atttggcatg gattttggtg cttcgattac ttgtaaagag
      481 ccatctggtg cttctgcaga aaggatcatt ccttgactga tttgtccacg catctttcgt
      541 ggtttcagat ttgctacaat gacgactttt ttcccgatca attcgcttgg atcaggataa
      601 aattcagcga ttcctgacaa gatttgacgg tcttggctgt ctccagcatc tagtcggaat
      661 tgcagcaact tatctgcccc ttctactttt tgacagttga tgacttctgc tacttttagt
      721 tctacttttt caaatacatc aaatttgatt tgtttttctt tcgttgaaac aagttctgtt
      781 tcttctggat cccatttgac tgtatcttcg tttgtttgag taccttcaga cattttttct
      841 tgaataaaag caacttcttc ttccatatct aaacgtggga agattggtgt accttttgca
      901 actacttttg ttcctgaagg aaattcgcca aagtgtaatc cttcaagatt cattgtttca
      961 gagtccaatc ctagttgatt gaagatcttc tctggcgttt ctgtcatgat tggctgaagt
     1021 aatatcgctg cgatacgcaa agattcagct aaatggatca tgacactatt caattcagct
     1081 ttcttttctt cgtcttttgc taatacccat ggttctgttt catcaatgta tttgtttgca
     1141 cgagaaatca gcacccagat ttcagccaat gctgtattga attccatttt ttccattgca
     1201 tcatggtaac gtccgacaac gttagcagcc gtcgtagaca gttcactatc aaatggagtg
     1261 acaagtgagg catagtcagg aacgagtcca tcacaatatt tgttgatcat agcaatcgtt
     1321 cgattcaata agttccctag atcatttgcc agatcataat tgacacggga aacaaaatct
     1381 tctggcgtaa agacaccatc tgacccaaac ggaacagcac gaagcaaata ataacggagt
     1441 gcatctaaac cataacgttc cacaagcatt tcaggataaa cgacatttcc ttttgattta
     1501 gacatttttc cgtctttcat caatagccag ccatgcccaa atactttttt aggtagaggc
     1561 aaatcaaggg ccatcagcat gattggccaa tagatcgtat gaaagcgaac gatttcttta
     1621 ccgaccatct gaacatctgc aggccagtat ttttcgaata gactttcatc ttcggagcca
     1681 tatcctagtg cagtaatata attggataat gcatcgatcc atacatacac cacatgtttc
     1741 ggatcactct tgactggtac tccccaagta aaagttgttc gtgaaactgc aagatcctct
     1801 aatcctggtt tgataaagtt gttgatcatt tcgtttttac gagattctgg ctggatgaat
     1861 tcgggatgtt cttcatagta ttccaataga cggtctgcgt atttgctcat gcggaagaag
     1921 tatgattctt cttttaccaa ttctacttcg tgtccacttg gcgctttacc accgatcacg
     1981 ttaccttcgt catctttgta cacttctgca agttgtgttt ctgtaaagaa ttcttcgtca
     2041 gaaacagagt accagccttc atattcacct aagtaaatat ctccctgttc taataaacga
     2101 tcaaatattt tttgaacagc tgctttatgg taatcatctg tcgtgcgaat gaatttatca
     2161 taactgatat caagcgtttt ccataatttc ttgacatcag ccgccatttt atctacatat
     2221 tcttgcggtt ttacacctag ttcttctgct tttgtttcga ttttttgacc atgttcatcc
     2281 actccagtga gataaaacac atcaaagccc atcaaccgtt tataacgagc aattgcgtca
     2341 catgcaatcg tagtatacga gttgccgata tgaagtttgc cgcttggata atagattggc
     2401 gtagtgatat aaaaagtttc cttttctgcc acggggctct tcctccttta tcgcatgatg
     2461 cgaattcgtt tccttgctag tataccataa aaaaagcatg ctgttagctt tttttcaaaa
     2521 tgaaaaagac tggataaacg acttctatct acgttcaatc tatcctttaa aaggatgaat
     2581 aggttgaata aatgcgggtt tttgttttgt ttcatatcta ctaaaaaacg gctttttatt
     2641 tctcccctca cacgtggtat aataaagact atctatctta gtaagggact ggaacaatca
     2701 tgcagaagaa acatcttttt tttacactta gtattgcttt tctttcatta gcacatttaa
     2761 tattcagcta tttttatatt cgtatgtatg gttattttaa cctccatggg catttgaaca
     2821 gttttatgac agcagcttgg attttgcgtt ttatcattga cgtatatatc gttatttgtg
     2881 gcttctttgc cattcgtgaa gagcggtata aagtattgcc gttttatctg cttttctttt
     2941 tattcaatct catacttcca tttatttttc atatttaaat gataacctag atttttcaag
     3001 taaatccttg gttttttttc agagtctgct acaataaggt aaaaagtact tttactgtca
     3061 atggaggtgg tcagatgaac cgttttgctt ctaaagaaga agaaaaatta tattatgaac
     3121 ataaagcaag tgaagaagaa tttcttaact ggtatcaaaa acaggaacgt ccagaatatg
     3181 acaagccctc tttgactgtc gatatcgtac tgatgtgcta taacaaagaa gaagatcagc
     3241 tgaaagtttt gttgatccaa agaaaagggc atccttatcg aaattcatgg gcattgcctg
     3301 gtggctttgt ccaaaaggat gagtcaactg gagaaagcgt cttaagagaa acaaaggaag
     3361 aaactggtgt ggttatttca aaagaaaata ttgagcaact gcatacattc agtacgccta
     3421 atcgagatcc gcgaggctgg gtcgtcacag tcagctactt agcctttatt ggagaagaac
     3481 cgctaatcgc cggagatgat gccaaggaag tacgctggtt cacactagaa cgtcatagta
     3541 acaagatcca tttatctagc ggagaagtgg ctatctcgct tgatttagtc actggagagt
     3601 cttctggcaa agatactttg gcttttgacc atagtcaaat catcttgaaa gcattcaacc
     3661 gtgtagtcaa taaaatggaa cacgagccgc aagttctaca agttttaggt aaagatttca
     3721 cgattacgga agctcgaaaa gtatttgcta aattcttagg gatcgattat aaaacgatcg
     3781 atcattccaa ttttaagaaa gctatgctcc aatatttcga tgagatcggt gaacgtcctg
     3841 taggaatcgg ccgtccttca aaaatctatc agctcaagcc ggggcaccat gaataaggtt
     3901 gtgagcaaaa gattcagacc taatcattca agaaaaaacg caaaaacaac tctttgtgag
     3961 ttacgttttt tgtttcagaa ttttgatgta ttcgtaaact ttctgaataa aaaattaaca
     4021 atagcagaaa aagctcgtac gaactttttc tgctattgtt ttattggttt gtcataatta
     4081 gtgagctttt cttcaaaaga aaaattccta gaaacaaaag tcattaagac tttttattaa
     4141 caaggctaac cctaacgctc ctatcagttt cattcatttt ttatcccttg ataaccgaat
     4201 gtctgcccat tttttctact acttggctct ttcttacgga attgctttct tctgctccgt
     4261 cggtattgat caaaagaaat tttttattat accattggga cttataacgc aattttgtta
     4321 ttaatttatg ccagtcgtga atagaaaaat cagcatgtgc ctcttctttt ggaaaagatt
     4381 ctacaaataa gtagtcatat tgtttgacca atttcccaga taattgttca agataatctt
     4441 ctcgctggtt cttttgtttc agataaagat ccattacttt tctcttttgt ttctgataat
     4501 tgcttgcctg atctaacctg atctttctat ggtgagcgga gcggactttc acattcaatt
     4561 tccgctgtgc atgttgcagc ttttcttctg tctggtgaaa ttttggtaaa gttacttcta
     4621 gaggtcgaga tgtttggatc aatgtcttcg gatgataggc gatcccgatc catttagcta
     4681 aagattcttt ttttactggc gaaacatcac aaagtagtgc gacataaaat tcttcattat
     4741 atcgagcaga aatcgtagcg gagcgaatcg atccttcaac tggccgatgc tgatttatct
     4801 tgattagttc tttttgtttt ggtaacttta agtagccatt ttccagatag atcgtacggg
     4861 tttggttgtt ggtcgtataa gattgccaca tacttttttt cgttttaagc tttgggaagc
     4921 tggcacgtcc cttgaaataa ttgcgaaaag cccgatccaa attaagctgt gcatttgcta
     4981 aagcaagact gtctacttct tttaagaatg ggtattcttt ttttaatgta gcgggtgtga
     5041 gcgaaaaatt ggctactcct gtttgttgcc aacttttctg ccgctttttc agcaaatggt
     5101 tataggtaaa tcgagcacat ccaaaagtac gaatcaattg ctgtttttgt tcttctgtcg
     5161 gatacatacg gaatttaaaa gcttttagca tctatgtccc tcctagttag caaccagaag
     5221 agattccttt tgtcgtttta gtttttttgc gtaatatggg acttcctgct cagttgcata
     5281 gacaaaatgt tctggtgcga tttcacgaag ttgacgtact tgtccgtcat ctggttcagg
     5341 ttgatatttg attcttctgc gttttctctc atggtctgga tctggtagag gaatagcaga
     5401 taacaaactt tctgtataag gatgaacacc gtgattatag atttcatcac ttgttcccat
     5461 ctctaataac aagccgttat gcattacacc gattcggtca ctaatatgtt taaccatcga
     5521 caaatcatgt gcaataaata gataggtcaa ttgatgttct ttttggagat cttgcaacaa
     5581 attgaccact tgggcttgga tcgacacatc tagtgcagaa attggttcat cacaaatgat
     5641 aaaacgcggt tttactgcca atgctcgtgc aataccaata cgctggcgtt gcccacctga
     5701 aaattcatga ggataacgag taccgtggct tggattcaat ccaacagttc tcaatagatc
     5761 atcgactttt tttgcacgct cttcagatga tttagctagt ccattaacat caattccttc
     5821 ggcaatgata tctctgactt tcatacgagg attcaacgaa gcgtatggat cttgaaagat
     5881 catctgaaca tcatgccgga atttcgtcaa acctttttta tctttgatct tcgttatatc
     5941 ttgaccgtcg aataatatct caccatcagt aggttcattc aagcgaatga tcgtacggcc
     6001 agtagtggat tttccgcttc cagattctcc aactaaaccg aatgtttctc cttcataaat
     6061 gtgaaagctg atgtcattta cagcatgtac ttcattcttt tttcctacat tgaaatactg
     6121 tttcaatcct ttaacttcaa gtagtttatt tggcatcagc aattcctcct tgaccgtaag
     6181 tatgtttttg tttctcttta aagatagccc agcgacgttg gatttcttct ggcggtgtca
     6241 ctttgggtgc ctgcggagca agtagccaag ttgctgcctt gtgtgttttt gatagttcaa
     6301 aaaatggtgg ttcttgttct gcatcgattt tcatcgcgaa ttcatttctt ggatagaatg
     6361 cgtctccttt tggcgggttc aataaatccg gtggagaacc aggaatagca tacaatcgat
     6421 cgttcgcact atctaatgtc ggcattgaac caagcagtcc ccaagtatag ggatgttgcg
     6481 ggttgtagaa aatttcttcc gatgttccaa cttcaacgat tcttcctcca tacatgacag
     6541 cgacacgatc agcgacattc gctactacac caaggtcatg agtgataaag ataattgatg
     6601 tatcgatttt tgtctgaatt tctttcagca gctccaaaat ctgtgcttga atagtcacat
     6661 caagcgctgt tgtaggttca tcagcaatca ggatttccgg ataacatacc agtgcaatcg
     6721 cgatcacgat ccgttgacgc tgacctcctg agaactgatg cggataactg ttgatacgtt
     6781 tctcagcttc gggtattcca actaatttaa gcaattctaa tgcagctttt tgtccctctt
     6841 tttttgaaac tttattatgc tttctaaggg actcagcgac ttgcttccca atgggcatcg
     6901 tcggatctaa agaggtcatt ggatcttgga aaatcatcgc tatttcttta ccacggattt
     6961 tttgcatctc tctttctgat tttccaacga tatcgttacc tttaaagagg atttgaccag
     7021 catctatatt ggcgttgctt gacagcaagc gcatgatgct tctggtcgtc actgattttc
     7081 cacttccaga ttccccaacg atcgccaatg tttctccttt attcaaatga aaattgacac
     7141 cgcgaatcgc tcgtaccttt ccagcatacg tatcaaaaga gatttctaag tctttcactt
     7201 ctaaaatttt cgtcatcgtc ttcactcttt cattttagga tcaaaggcat cacgcaatcc
     7261 atcagcaagt aagttaaagc caatcatgat aaccgataaa gttactgccg gaatccataa
     7321 tagataaggc agatacagga atgtcttgta cccttcgctc aacatcgttc ctaatgaagc
     7381 tgacggaggc gtaagaccta aaccgataaa gcttaaaaag gcttcgaaga aaatagcaga
     7441 tggaatacta aacatcattt gaataataat gacactagag atattcggca aaatatgttt
     7501 gaatgcaatt ttcattcttg attcacctaa tgtcaatcca gctaaaacat attcttgatc
     7561 ttttaatttc attgtttgtg cacgaacgat acgtgccatt ggtatccagt ttgtgatggc
     7621 catagcagcc acgattgaaa aaattcctgg ttcaaaaacg accaacatca aaatcatgac
     7681 aacgaggttt ggaattcctg ataagatttc caaaaaacgt tgcatcacgt tatcgacacg
     7741 tccgcctaac aaaccagaaa ccaacccgta agctacacca atcgttacat ctagtaatgc
     7801 agcaataaat gcgatcagca acgagattct cattcccata aataagcggc ttaatacatc
     7861 acgtcccaaa ccgtctgttc ctaaaaagaa atttacgttt gaagggacgt ttgcttgagc
     7921 atatttatca acaagttcac caccaaccat tgcttttcca ttcaatccat cgatattgat
     7981 tcccggaata cgaggaggca aattgatata agccacgttt tgggcagttg gatcatgagg
     8041 agaaacaaag attgtgatca cagagatcaa aatgatgacc aatagtaaaa ccattgaaat
     8101 aacggcagct ttgtttttct ttaaacggcg ccatgaatct tggataaaat tcaaagaagg
     8161 tgcagtaatc tgttcacgtt cttcttcggt atttttttgt aatggaagaa attcatccgc
     8221 agggatatct gcaactgttt cataacgctt tggtatcatt tccatttatc ctcggctccc
     8281 ttctgaaaca cgaattcttg gatcaactag accgtacaat agatccacga tcaaaataac
     8341 aaaaatcagc ataaatgagt aaaggatcgt tacagccatg atcgtaggat aatcatttgt
     8401 tgtgattgac ttaacgaatt gttccccgat accaggaata gcaaagatat tttctaccac
     8461 cagtgatccg gtcatcaatg ctacagctaa cggtcctaat aaagtcatca atggaatcaa
     8521 actatttcga agaccatgtt taaaggcgat ttcccaacgg ctcaatcctt ttgcttttgc
     8581 taattccaca taatcactat gcagtacttc caccatttcc gtacggataa accgagcaga
     8641 gtcagcaagc ggcgacatgg ccaacgcaat tgttggtaag attgtgtaca caaagccatc
     8701 ccattttgcg attggcaaaa cttgtaattt gattgcaaag atatattgta ataaaacagc
     8761 aaatacaaag tttggaatgg aacgacctaa tatcgctacg agtgtagaag atgtatctgc
     8821 ccatgtattt tgcttcatgg cagagatcgt tcctaaaatc gtccctagga ctgttccaaa
     8881 gataattgcc tgtaacccta actgcaagga tggtccgatt cttccagcta acaaatgtgc
     8941 gactggctgg tttttaaatt ggaaggaaat tccaaaatct ccttgaagca aattagaaag
     9001 ataaatccca tattgtacga taactggttt atccaaaccg acttgtttat ttagcatctc
     9061 gattgtttca ggactcaaac gttcctgatt cgtatatggc gtgcccggca gcaattgcat
     9121 caagaaaaag gtgattgttg caattaacca aagcgtgatg atcatgaaaa agacacgttt
     9181 taatacatat ttgatataac tattcactaa tgtttccccc tagcttttga cttctgacag
     9241 atgtttcgct acattattag ataatgaacc aaaaataaag gagcaatcaa atgaaaaaaa
     9301 aatctatccc ctacgcagta gcttttctac tgattctggt tattttaata aaaaatctca
     9361 tcaaccattc ctttacactc attcaattat ctaatgatct attcttatgg tcattgccgt
     9421 ttctgattat cggcggtttt ttatgggtct tttcttcggg tttcttcgat cattttcagc
     9481 gatcggtcca tcttgcacga acaaggaacc gaaagaaaaa accagaattt tcctcgctct
     9541 cgtctgccag ctacggtatg tacagctttt ggctgatcat tgccggaata ctaattgctc
     9601 tttctgctat tttcatgctt ttttctttgt taggataagt gaatatgaga acgaggttgc
     9661 gacatttgcc gcagcctcgt tttttctcat gctcacggta ttaacgatta ttcttcaacg
     9721 tatgtccatt tgtaatccca tgaagcgcct gccgcatggt gaacgatacc tttgattttt
     9781 tcattcacta ggtgaccttc tgctttttgg tacaccggaa cgacacccat atcatcagag
     9841 atgatcttga ttgctttttg gaaatcatcc caacgggctt gttcatcgcc agcgtctgtt
     9901 gatgaagctg ctttaactgc ttcgtcatat tctgcattgc tccagcgacc acggttgtat
     9961 gaattacctg tgacaaacaa atcagtaaag ctgcttgggt ctgcatagtc agcagcccat
    10021 ccgcctagta ctgcgtcaaa atcacccgaa gttgagcggt caagacgtac agagaatgga
    10081 acaggtgttg gtttgactgt tacgccatcc aagttttctt ggattgagtt ttgaacatat
    10141 tcgattactt ttttcgatga atcatcatca gaagccatca aatcaaattc taaagaatca
    10201 acgcccaatt cttttttcgc tttttcccaa tattcttttg ctttatcttt gtcataagaa
    10261 actaattttc cagcttcttt tgcaaagtct tcgttcgttg ttggactttt taccatatct
    10321 gatgggatca atcctgtaga gacaactgat ccgtcaccta gtacttgttt tactaaagct
    10381 tcacgatcga tagaataaga aatagctttt cttaggttca cattgttgaa tggtgaattc
    10441 ttttcacgtt gattgaattc gatatagctt gtccgcgctt ctttaacaga agtataagct
    10501 ggatcgtttg cgttttgctg tgctaactca ccagacaaga tcacgtcatc tgcttggcca
    10561 tctttgaata agttcaatgc agtagaagat tcttttacta cactgacttt gatttcagaa
    10621 agttttactg tatctttgtc ccagtaatga tcatttttct tataagtcca ttctgtatct
    10681 gttcctggtc catcaaaatc ttctaatgtg aatggtccat tataaacagc tttatcactt
    10741 gcagatgcat aggcatcccc attttcttcc actacggatt gtttttgtgg gaaaaatgat
    10801 gggaaagcaa gcagatattg gaaatatggt gtttgtttcg tcaatttgat ttctaattca
    10861 tagtcaccaa ctgctttgat ccctagttct gatttatctt tctttccagc tgtaatgtct
    10921 tctgcatttt caactggcgc atacaggtaa gcatattcag aagctgtagc tgggtctact
    10981 gttcgttgcc agccataaac gtaatccgct gctgtaacag gatcaccatt tgaccatttt
    11041 gcgtcttctc gtaatttgat tttgtaagtt aatccgtctt cactgacttc tgccaactcg
    11101 ctagctcctg ccggctgtgg tttgctgtct tcatcaagac ggtaaattcc ttcataaacg
    11161 ttattcaatg ctgtaaaact gattgtatct gttgcaactg ataagtcagc tgtaggcatt
    11221 tcttgaggca cgactaagtt aaatacgcct cctccatctg ctgaaccgct ttttgctgaa
    11281 ctactgcttg tggctgaact gccgccataa catccagcaa gtacaaggcc gcataccgca
    11341 accaaaccaa atgtaaattt ctttttcatt ttacgtaccc ctttattgga attttcagaa
    11401 taattaatat tttctgaagt tgtttaagag tttaatgaat aattattaaa aaatcaacac
    11461 ttttttttgt ctttttcata aaataatgat tttttcgtga ggtttcaagg aatttttcag
    11521 aaaaaatagc tttgtacaag gttttccatg gtcttccaaa agccattaaa taattttcgt
    11581 tatattgtct attattgcca tacaaagata aaaaagaata aacaaaaaga aactgtgaca
    11641 aaagtcttgt cacagtccct tatctga
//
LOCUS       DACDNO010000075        11408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDNO010000075 DACDNO010000000
VERSION     DACDNO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.075"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(115..450)
                     /locus_tag="IWI22_002348"
     CDS             complement(115..450)
                     /locus_tag="IWI22_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9169717.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            736..2616
                     /locus_tag="IWI22_002349"
     CDS             736..2616
                     /locus_tag="IWI22_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9169718.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            2695..2922
                     /locus_tag="IWI22_002350"
     CDS             2695..2922
                     /locus_tag="IWI22_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9169719.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            2927..3469
                     /locus_tag="IWI22_002351"
     CDS             2927..3469
                     /locus_tag="IWI22_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9169720.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            3720..4397
                     /locus_tag="IWI22_002352"
     CDS             3720..4397
                     /locus_tag="IWI22_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9169721.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            4718..5959
                     /gene="sstT"
                     /locus_tag="IWI22_002353"
     CDS             4718..5959
                     /gene="sstT"
                     /locus_tag="IWI22_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9169722.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            6106..6741
                     /locus_tag="IWI22_002354"
     CDS             6106..6741
                     /locus_tag="IWI22_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9169723.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            6932..7675
                     /gene="truA"
                     /locus_tag="IWI22_002355"
     CDS             6932..7675
                     /gene="truA"
                     /locus_tag="IWI22_002355"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9169724.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            7909..8742
                     /gene="msrA"
                     /locus_tag="IWI22_002356"
     CDS             7909..8742
                     /gene="msrA"
                     /locus_tag="IWI22_002356"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9169725.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            8823..9816
                     /locus_tag="IWI22_002357"
                     /pseudo
     CDS             8823..9816
                     /locus_tag="IWI22_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            10105..10803
                     /locus_tag="IWI22_002358"
     CDS             10105..10803
                     /locus_tag="IWI22_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9169726.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            10953..>11408
                     /locus_tag="IWI22_002359"
     CDS             10953..>11408
                     /locus_tag="IWI22_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9169727.1"
                     /translation="MRGSGMSFMDKLAKVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISYLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTK"
BASE COUNT         3762 a         1851 c         2377 g         3418 t
ORIGIN      
        1 gtaaccgata cattagtttc aatgtttact gagtaactaa ttattttgca ggaggacaat
       61 ttatttacac aaaattattg acgctcccaa aatactcgtt tacataaaaa aagattattt
      121 agatgcgata atatcataga taaagaaaca ttcatcataa gaaacagtga tcccaaattc
      181 tgtttcaata tttttgaagg ccttttttac atgaaaaatt gtttcatgag aaggtcttgt
      241 gtcatcttct gatataattt taagttcatg accgtttatc aatgtacgct caattagaca
      301 cgctgtatga atcaaaagtt ttaaaagaat ggaatgagtg aattttttgt ggaaagtaca
      361 ttccaattct tggataaaat ccattaagct tttttggatc ttattcccag ctaataagta
      421 gagatatttg tccactgctt caactcccat atcaaaaaac gatatttctt gtgaatgagt
      481 ggctgctgcc cttttttgat tgttgttctt cagtcgtaag atgacaaagc atcttatctt
      541 tgaataaaaa aatagaacta cctaaggaag cgtttcggcg cttccttttt ctatatgatc
      601 gtttaatgac tgtaggaatt cagtatacca aaagaaaacc gcctttttga ataaacttga
      661 tagtggtcaa ttttttatga tggataagta tctatactta gtctcgtaag aatgaaaagg
      721 aaaagaggga gaaagatgaa attacaaaag tggatccaag agcatcgaaa tcatatcacc
      781 gccttatcag ctgtgttgat tatttttggt tttatcggga aatttactgg atttgtcttg
      841 ttctataaca ttgctctagc catagcttct attattgcgg tcatacctat tataatccat
      901 gcgtatcagg cagtaaaagt caaagttatc tcaattgatt tactcgttac tattgctgtg
      961 cttggtgcct ttatgatcgg agaatacaag gagtcagcta tcgttacatt cttgtttttg
     1021 tttggtagtt atctggaaca aaaaacattg accaaaacgc gggaatcaat caaggctttg
     1081 accgagatgg ccccatcgac tgccagcgtt gtccaagaag atggaacgac cgaagaagtg
     1141 gaaatcgatg acgtggaaga aggcgatatc gtcttagtaa aaactggtgg agctattcct
     1201 gtggacggac gagtcattga cgggaaaggg tatgcggatg aagccgcagt gacgggtgaa
     1261 tcacgacaag tcgtcaaaat cagtggagat aacgtatttt ctggaacgat tttagcagat
     1321 ggctacgtga cgatcgaagc aactaaggtc ggagatgata cgacctttgc caaaattata
     1381 gaattagtcg aagaagcaca agataccaaa tcacatgcgg aaaaattcat tgatcaattt
     1441 tctcagtatt atactccaac cgttttactt attgcgttgc ttgttggtct gatcagtcgt
     1501 gattttcgtt tagccatcac tgttttggtt cttggttgtc ctggcgcttt agtcattggt
     1561 gcgcctgttt cgaatgtagc aggaatcggt aatggagcga aacggggcat cttagtcaaa
     1621 ggcggtgaag caatggatac tttttccaaa atcgatactt ttgtctttga taaaaccggt
     1681 acattgacca aaggaactac tgctgtatcc actatcaaga actatacaga ggatgaaggg
     1741 gacagcttag ctttagtggc tcgattagaa acgttatccg atcatcctct ggggcgcgcc
     1801 gtgatccatt atgctcagga gaaagacatt gattttcaac aattagcagt caaagataat
     1861 cagacgatca agggacaagg aatcacagct gagattgatg gtcatacagt ttgtgcgggc
     1921 aacaccaaat tgattgccgc tcacaaactt tctctcacac caaagcaagt acaagattta
     1981 catcaattac aacaaagagg cagctcagtc attattgtag caatcgacaa aaaaatcact
     2041 caaatcattg gtgtgtcaga tgtgattcga ccagaagtcg ctgaacaatt agctatgttg
     2101 aaacaagcag gagcaaaaca tttagtgatg ctgacaggtg ataatcagat gactgctgat
     2161 tatgtagctg acatgttagg cattgatgaa gtccatgctg aattattgcc agatgaaaag
     2221 gttcaatttg tgaaaaaata ccaagaacaa ggattgcacg ttgctttcat tggagatgga
     2281 atcaatgatt ctccctcttt ggctgcggct gatatcggga ttgctatggg atcaggaacg
     2341 gatgtcgcta tcgaaacgag tgacgttgtc ttgatgcaat ctagttttgg atcgttggtt
     2401 catgcttacc gtttggctaa gaaaactgtt ttgaataccc gtgaaaatgt agtgattgcc
     2461 attggtgtcg tcttattttt actcatcggc ttatttgccg ggtttattta catggctagt
     2521 gggatgttcg ttcacgaagc tagtatcttg atcgttatct ttaacgcgat gcgtttattg
     2581 tattttggac acgaaagcaa agaagaaaaa tcgtaaaact tgataagaat caattttttt
     2641 atggaagctg taatctagaa taagactatc aagaaaaagg aaggaagaac tacgatgaaa
     2701 aaaatgatta tgcaattaga tacactagct tgtccaagct gtatgcaaaa aatagaacat
     2761 gcggtttctc aacagcctgg ggtaagcaat acgaaagttt tgtttaatgc gagcaaaatc
     2821 aaagcaaact ttgatccaga tgaagtccac cctgaacaat tagctgcagt tgtggaaaaa
     2881 ttaggttatg aagtccaatc aacaaaagta aaagaggcgt aaatcagtga atatagtgga
     2941 agaaaaattt gctcaagaaa caaaacaagc agatatcgat catcataaac caactgctgg
     3001 cgccatggtg gggcatgtat tggcaaattt taaattagaa gaaatgaaat tgatccaagc
     3061 acaattgtat gttttgggta catcttcgca agaattaagt accctttttt cagaatatgc
     3121 tgatcaagaa gcgagatggt tcaataaaat ctctcatgag ttagtgattg aaaatgaaat
     3181 cgttccgtct actttagaag aagtcatgcg ttatggaaaa atttacgaac atggtcaaaa
     3241 caaatattta ccagcctcaa caatgttgga aaactttgca aaagattttg atttccaaaa
     3301 tgtctttatc acacgggcaa tcaaattagc tgaaaaagaa gaaaaatatg gcttgaaaca
     3361 attgcttgtt gaacttttaa gtttcaataa agaaatcatc taccaagtac aaagactcca
     3421 aggaaaaact gtccgacaag atttggatga ggaagacgat gatgactaaa agtctatgtt
     3481 tcttcaaata caaatataaa gcgtgaggta aattggaaaa tcgcgaagat taaaataaaa
     3541 gaagcacccg ctgatgtatt acagacaaca tcagcgggtg cttcttagtt tttaaaaggt
     3601 ttaaaagatg gatgagaaaa tacaaaaaat caataaaatc acattgctta tttgatagaa
     3661 taaagaagtg ggagtaatcg aaaggagttt tttaatttaa aaaaaggatt ttagtaacaa
     3721 ttggtctagc ttttttgtta gctggttgta ccaacgggaa tgaatcgaaa aaagaatcaa
     3781 caaaagaaac agcgaaagca acaattgcaa ctactacaac aactagcaca gagagcaagc
     3841 caactgcagc aagtacggaa acagcagata cctctacttc gtcagagtct tccaaaacga
     3901 ctgaaacaac tcaaacatct acttcaagtt cttctgtaga ggcttcatct tcagaaagca
     3961 gcgaacaagt cgaacaaaca ctgtggaatg cagacaaatc cgcacaatta gaggcattta
     4021 tggctacatg gggccaaaca atgggacaac aatacaaaag ctatacgaat caaatgagtg
     4081 tggacttata tggtcttaaa gtccctcaag ttattttgaa cggggagtgg aagatggcta
     4141 ttggtggagt gcctgtttct gcagaatggt cagaaagtgg gacaggtcaa gcagactatc
     4201 aaatcacagc cgtctattcc gatgcagaga cagaaccata tctgaaaaag cacgtctatc
     4261 tattcgggtt tcagcaaaat cagccaaaag tattggtgac acaacaaaat caggggaatc
     4321 ccgataatta tctttacttt aacgagacag cgaataatga attgaaaaat ggctttaatc
     4381 aaatcgttta tgggtaagta aaccagtgag agtgagagaa agaagagact cagtggaaaa
     4441 tattaattga ttgctatgtc taagaattaa gatgaaggat gaaatttcta gtttcgtcct
     4501 tcatcttagt tacgttttac ttggataact ataataataa cgataaatag ttttctctcg
     4561 acttctttag agattgaata aaccctccaa tactaaaatt gaatgttgca agagcatgaa
     4621 ttgccaattg tattattaga atttttagaa aattgcacct ttagtttctt tagataaaat
     4681 gatacaatga ttgttaaaat tgtgaaaggg tggtcatttg ttagagaagt ttttggatat
     4741 gtcgttagtt caacgtattg ttgttggtat tttagtcgga atattgtttg gtatcttttt
     4801 ccccacgtgg acatttattt caatattggg gacgttgttt gtcagttgct tgaaggcaat
     4861 cgcacctatt ctggtttttt tcttgacgat ggcgtcaatt gccaagcata aaattgggaa
     4921 taagacattc gtgaagccta ttttgatctt atatttagtg ggaacattgc tttctgcact
     4981 tgtagcagtc attgtaagtt caatttttac tgttcctatt actttacaag aaacagtatc
     5041 tgaaaaagca ccgcaaagtt tggaaagtgt tttatctact atgttgacga atgtcacaca
     5101 aaatcctgtt caatctctta ttgatgcgaa ttaccttggc gttttattct gggctgtatt
     5161 gttgggacta gcttttcgag cacgttcaga accgacaaaa gagttggtgg atcagatatc
     5221 tatagcgttg tcacaagtcg tacaagtgat tattgcattt gctccgatag ggattttagg
     5281 attggtttat caatcaattg ctacgactgg aatcgctgga ttggcagagt atctgcagct
     5341 tttacttgtt ttgatcggta cgatgctgtt tgtcgcatta gttgtgtacc cattcctcac
     5401 gtttttgttt attaaagaaa atccctatcc attgattttc ttttgtttga aagaaagcgc
     5461 acttcccgct ttctttaccc gaagttctgc cgcaaatatc ccgataaaca tgatgttggc
     5521 tgaaagattg aaattgacaa aagagtctta ttccatatcg atcccgttag gtgctacgat
     5581 caatatgggc ggtgccgcga ttaccatttc cttgatgacg ttgactgctg tacattcttt
     5641 agggattgaa gcaccatttg ttttgaaagt ccttttatgt attttgtctg ctttagctgc
     5701 ttgtggagct tcaggtgttg cgggcggttc actgttattg attccattag catgtagttt
     5761 gtttggtatc tctaatgatg tcgcgatgca aatcgttggt attggattta ttatcggtgt
     5821 tatccaggat tcaatggaaa ctgccttaaa ttcgtcaagt gatctgttgt ttactgcaat
     5881 aggagagcta ggcgcgcgta aaagaagtgg ggaaaaaatc aaattgaaag attgtttaga
     5941 tggattaagt gaacgttgaa cgttagatat ttagtatttt taaaatgatt cgagttttta
     6001 atgagttata tagatgttcg tgtaaataga ggtttctgtt aaatcaagat aaagtcgttt
     6061 ataataacaa ttaaaagcat aggtaattgt tattggaggc gtatcatgga ttttttacgt
     6121 aaatttggtt ttttcttttt attattaggc gtgctgagtg attttttgac tccgtacgta
     6181 ttaggattgt attatcctga attagatcaa atgaagagcg tgatcagtgt atttggagag
     6241 gtttccagtc ctgtaagaaa agcgtttctt atttggtctg tggtttcagg gatctttttc
     6301 atcctgtcat tgcctgcatt gtatgctgca tttgtaagta catctcgtgt gttagctacc
     6361 ctagttacag cagccatcgc gttctatggt ataggtgatt gtatctttac aggtttgttc
     6421 agtatcaata cccatgaatc ttcgtggaat gtctcgacat gggtccataa tatcggatca
     6481 ggtttaggtt acagtggttt tttattgttt ccagttcttc ttttcttgat ctatcgaaaa
     6541 aatgggcaaa tggacgtaag ccacttatac cttggactgt ctgtgatcag tttgcttttt
     6601 gcggcgattt atggactggc aagaattcct tctataaatt cttggccatt actgaataaa
     6661 ataggttttt gccaaagaat cagttttttc tttaattatg tgcccattgc ctggttgagt
     6721 atcttgcatc tcaaacagta aaacttgagc cgtcaaattg aacagtcagc ggatacttca
     6781 tccggtggct gttttttgtt tttctgaaat atatatttcc acagttttct tgtatagctt
     6841 ctgtcttaga gaagcaagga gctatatttt gtgctacaat cttaaaaagg tagagagaag
     6901 accaaatgga agacagagga gccactaaaa tatgagaaac atcaagttga caatcgaata
     6961 tgacggaaaa agatattcag gatggcaaag acttggagat gacgataaaa ccattcaagg
     7021 aaaaatcgaa aaagtattgc atcagatgac aaatgaagag atagaaatta ttggatcagg
     7081 aagaacagat gctggcaccc atgcgcgagg acaagtagca aattttaaaa caaacacaga
     7141 aattggactg gcagaaatga tcgactttat gaatcgttac cttccgcggg atattgtcat
     7201 caaaagaatc gaagaaatgc ctgagcgctt ccattcaaga tataatgcgg tgggcaaaaa
     7261 atacagctat tatgtttgga acaacgtgat tccttcagca tttgagcgaa accacagctt
     7321 ttatttccct caagagttgg atatggacaa gctgaatgca gcttgcgaaa aattagtggg
     7381 tacacatgat ttcattggtt tttctgcatt gaaaaaatca aaaaaatcca cagtgcgtac
     7441 gattgatgaa ttatctatcg aacgaaaggg agaaatgctt cattttactt ttgtcggaga
     7501 cggattctta tataaaatgg tgcggattat catggggaca ttactagaaa tcggcacagg
     7561 tgccatgccg atagaaacaa tcgatgaaat atttgaaagc aaagtgcggc aacatgcagg
     7621 agaaaccgtc cctgcacaag gtctgttttt ggatgaagtt tattatcgct cataaaaaag
     7681 aatcatcttt actcgacttc tgagaattgg agaacatccc ttttttcaga ggtcgttttt
     7741 tagttaaacc aaaccataga gtatgctttt taagataaag atcatcttat atcatctgaa
     7801 gctgcttatc ttctttgaat aaatacgatt caatcgatta tttcctcact ttatgataaa
     7861 ttacagctaa agtcactagc ttgtcttctt cggaaaggag gagaatctat ggatgaggaa
     7921 caaatattca ttcaaacact ttataatctg attttgaatc cgaatacacg agattgggaa
     7981 cgaaaagtat tgattcagac aaagaatgac acaagagaaa atatatccgt taaagaacag
     8041 ttatcaaaac tagaagtaac actacgtcca ttagctatcc gaatgaatct gacgcctgat
     8101 gtaatggact tttatttatt gattacggaa ggatttgata aggagcaaaa atatgacttt
     8161 tccaagcatg ccatgcaaga tgctgactat caagaacgtg cagtatttgc agggggatgt
     8221 ttctggtgta tggtcgagcc gtttgaatcg aaaaaaggga tcctatctgt tttgtcagga
     8281 tacacgggcg gacatgttga aaaaccgaat tatgatcaag taagtggtgg ttacacaggt
     8341 catgtggaag ctgtggagat catttatgac acaagagaaa taagttatag cgagttgctg
     8401 acaatctatt ggcaaattac tgatcccaca gatacatttg gacaatttca agatagaggg
     8461 aagcaatatc gtccagtgat tttttatcaa gacgaacgac aaaaagaatt agcggaacaa
     8521 tcaaaacaga aactagacag ttcaggaact ttccaccaac caatcgtcac aaaaatcgaa
     8581 ccagccggta cgttttggcc agcagaaaac tatcatcagc aattttacaa aaaacaaccg
     8641 aaacgctata aaaaaatcca gcaagcacgt aatcaatttc tgatctatca acgagtaaaa
     8701 aacaaatggc aaaaaaatat acgaaaaaat cattttgatt aactcctttt ctttaaaatg
     8761 tagtaaacta aaaatagaaa acaagaaagc gttttaaaaa gatttgatga ggaggagaaa
     8821 aattgtatat tgcagatagt gcacgttatg aaaaaatgga atatcgtaaa tccggtcatt
     8881 caggtttaaa attaccgatt ttatctctcc tctcggatta tggcaaaatt ttggtgatta
     8941 tgatcctatc cataatcaaa gagagatttt aagaggcgca ttcgacatgg gaattaccca
     9001 tttcgacctt gcgaataatt atggaggccc tgctggagct gcagagaaaa actttgggcg
     9061 tatttttaga gaagattttc aagcctaccg tgatgaactg attatttcta gcaaagcagg
     9121 ttatcatatg tgggaagggc catatgggga atggggctct cgtaaatcca tcatatcaag
     9181 ttgtgaccaa agtttccaaa gaatggggct agattatgtt gacatcttct atcatcatcg
     9241 cccagaccct gatacacctt tggaagaaac agcagaagcg ttgatgcaac ttgtaagaca
     9301 aggaaaagca ttatatattg gtatatccaa ctataatgga gaagatacaa aaaaatgact
     9361 gaaattttaa aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt
     9421 tctcgtgcac tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca
     9481 atcacgttca gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct
     9541 gaagattcta gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca
     9601 actctggaaa aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg
     9661 caaatagccc ttgcatggaa tttacgacaa aaatctgtta caagtgtact gatcggagca
     9721 agtcggttaa gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca
     9781 gaagaagaat tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat
     9841 ccatatatta aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg
     9901 aacatcatat tttttgtcga tcaaaataaa aatgatcgaa ggctaggagt gaattttatc
     9961 ctagtctttt ttatggtaaa tatcttcttc gtttgtatgt ctatgaacgg attaatcgat
    10021 tttttataca aaaaatcatc agatgattca aacgtgttat aataattacg aaaaaataca
    10081 tcagatgaaa aggaggtatg tggtttggaa gaaaataaaa ccctagtaga agtaactact
    10141 gacaagatcc ttagatattt aaaggaaatg cagttgcaag taggagataa actgccgaat
    10201 gaatatgaat tgtccgatta tttagaggtg gggcgaagca cggtgcgaga agctgtgcgg
    10261 gctctagctt ccaggaatat ccttgaagtt cggcaaggag cggggacatt tgtcagtgat
    10321 aagcgaggga ttgccaatga cccactcggt ttttctttga ttgaagatca ggatcggatg
    10381 atacgagact tatttgaact gagatattta ttggagccta agatggcagg tttggcagcg
    10441 gaatatgcaa cgaagaatca aatcgtccaa atgaaaaaat taacgcaaaa gattgaaact
    10501 tcttttcgag aaggcacaca agaacatgtt caattagaca tcgattttca tacgatgatt
    10561 gcagaagcta gcggaaacat agccttgctg catattatcc caatcattaa tgaatcgatt
    10621 gctttgttta ataaaaatta tgatatccag catctgaaaa tcgagacgat cgatattcat
    10681 acagagatcg ttcaggccat cgaaaggcgg aacgtacttg ctgcgtttga cgcaatgact
    10741 gtccatatgg caataaatcg gagagaatta cagaaaataa taggagaaaa agaaactaat
    10801 tgacagttca tctgatgtat tttatgataa atacatcaga tgattttttg ttttatattt
    10861 ggtaaaaaag aggagtaaat agtttctgtt caccaaaatg taagcgctga ctaatagaca
    10921 aaaagagatg acgtcaagga gaagaagcag gaatgagggg atctggaatg agttttatgg
    10981 ataaattagc caaagtttta gggaacgtgg caacgagaat caataattta aggtatatca
    11041 tggtgataaa aaacgcattt gctgcattga ttccggtaat tattacgggt gcttttggca
    11101 cattgttttc agcaatggtc tttgactcgg aaaatggctt agcacagata agttacttgc
    11161 gttttttaga agaattaaag cctatcgcat ctgccatcag ctatgtcaca ctaagtttct
    11221 tgaccatcta tgctgttttt ctaataggga tcgaattagc caagctgaat aaagtggatg
    11281 gtgtattccc tggtatcatc gcagtgatgt cttatttatc tgttaatcca actgtttatg
    11341 aattagtgaa tgaaaatcag actgttatag tagaaaacgt attggctaaa caatatacag
    11401 acacgaaa
//
LOCUS       DACDNO010000076        10952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDNO010000076 DACDNO010000000
VERSION     DACDNO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.076"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            38..126
                     /locus_tag="IWI22_002360"
     tRNA            38..126
                     /locus_tag="IWI22_002360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            141..212
                     /locus_tag="IWI22_002361"
     tRNA            141..212
                     /locus_tag="IWI22_002361"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:174..176,aa:Glu,seq:ttc)
     gene            228..300
                     /locus_tag="IWI22_002362"
     tRNA            228..300
                     /locus_tag="IWI22_002362"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:261..263,aa:Val,seq:tac)
     gene            310..382
                     /locus_tag="IWI22_002363"
     tRNA            310..382
                     /locus_tag="IWI22_002363"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Asp,seq:gtc)
     gene            418..493
                     /locus_tag="IWI22_002364"
     tRNA            418..493
                     /locus_tag="IWI22_002364"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:451..453,aa:Phe,seq:gaa)
     gene            495..575
                     /locus_tag="IWI22_002365"
     tRNA            495..575
                     /locus_tag="IWI22_002365"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Tyr,seq:gta)
     gene            582..655
                     /locus_tag="IWI22_002366"
     tRNA            582..655
                     /locus_tag="IWI22_002366"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Trp,seq:cca)
     gene            658..730
                     /locus_tag="IWI22_002367"
     tRNA            658..730
                     /locus_tag="IWI22_002367"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:His,seq:gtg)
     gene            742..813
                     /locus_tag="IWI22_002368"
     tRNA            742..813
                     /locus_tag="IWI22_002368"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Gln,seq:ttg)
     gene            859..929
                     /locus_tag="IWI22_002369"
     tRNA            859..929
                     /locus_tag="IWI22_002369"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:891..893,aa:Cys,seq:gca)
     gene            950..1034
                     /locus_tag="IWI22_002370"
     tRNA            950..1034
                     /locus_tag="IWI22_002370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:984..986,aa:Leu,seq:caa)
     gene            1096..1168
                     /locus_tag="IWI22_002371"
     tRNA            1096..1168
                     /locus_tag="IWI22_002371"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1129..1131,aa:His,seq:gtg)
     gene            1178..1249
                     /locus_tag="IWI22_002372"
     tRNA            1178..1249
                     /locus_tag="IWI22_002372"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1210..1212,aa:Gln,seq:ttg)
     gene            1267..1351
                     /locus_tag="IWI22_002373"
     tRNA            1267..1351
                     /locus_tag="IWI22_002373"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Leu,seq:caa)
     gene            1729..2481
                     /locus_tag="IWI22_002374"
     CDS             1729..2481
                     /locus_tag="IWI22_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9169728.1"
                     /translation="MGKGIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPI
                     EFMDFAEEVIELITARISSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLY
                     PNEYDIAIEVVERINHRYQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTIL
                     SHLEKSYSNVYDKESLTYHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCT
                     ALKIKDIIEQQFQLAIQENEIIYLAIHLARIY"
     gene            2585..3349
                     /locus_tag="IWI22_002375"
     CDS             2585..3349
                     /locus_tag="IWI22_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9169729.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3366..4778
                     /locus_tag="IWI22_002376"
     CDS             3366..4778
                     /locus_tag="IWI22_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9169730.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            4795..6119
                     /locus_tag="IWI22_002377"
                     /pseudo
     CDS             4795..6119
                     /locus_tag="IWI22_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            6132..6590
                     /locus_tag="IWI22_002378"
     CDS             6132..6590
                     /locus_tag="IWI22_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9169731.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            6882..8183
                     /locus_tag="IWI22_002379"
     CDS             6882..8183
                     /locus_tag="IWI22_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9169732.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            8210..8470
                     /locus_tag="IWI22_002380"
     CDS             8210..8470
                     /locus_tag="IWI22_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9169733.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(9469..10704)
                     /locus_tag="IWI22_002381"
     CDS             complement(9469..10704)
                     /locus_tag="IWI22_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9169734.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         3340 a         1768 c         2350 g         3494 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg caaactgtct caagggttcg aatcccttac
      121 tctccgtagc aaatttacct ggcccgttgg tcaagcggtt aagacaccgc cctttcacgg
      181 cggtaacacg ggttcgaatc ccgtacgggt cattttaatt gaatgttgga ggtttagctc
      241 agctgggaga gcatctgcct tacaagcaga gggtcagcgg ttcgatcccg ttaacctcca
      301 taatgaactg gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg
      361 ggttcgattc ccgtcggaac cgtcgttctt gttattcaat taataatttt aaaatatggc
      421 gcggtagctc agttggtaga gcaacggatt gaagctccgt gtgtcggcag ttcgattctg
      481 tcccgcgcca ccatggagga gtagcgaagt ggctaaacgc gacggactgt aaatccgttc
      541 cttagggttc agtggttcga atccactctc ctccatttta taggggcata gtttaaaggt
      601 agaacagcgg tctccaaaac cgttagtgtg ggttcaattc ctgctgcccc tgccatggcg
      661 aatgtggcga agtggttaac gcaccggatt gtggctccgg cattcgtggg ttcgattccc
      721 atcattcgcc ctttttattc ttggggtata gccaagcggt aaggcaaggg actttgactc
      781 cctcatgcgt tggttcgaat ccagctaccc cagtttctat aatttattct ttattttgta
      841 gattgataat agtataatgg cggtatagcc aagtggtaag gcagaggtct gcaaaacctt
      901 catcaccggt tcaaatccgg ttaccgcctt agtactaata aatcaacttg ccggcgtggc
      961 ggaattggca gacgcgctgg actcaaaatc cagtgcccgt tgagggcgtg ccggttcgac
     1021 cccggccgcc ggtattgact aagatgtctt catcttagtt ttttattgag aaattaaata
     1081 acatctttta tgatggcgaa tgtggcgaag tggttaacgc accggattgt ggctccggca
     1141 ttcgtgggtt cgattcccat cattcgccct ttattcttgg ggtatagcca agcggtaagg
     1201 caagggactt tgactccctc atgcgttggt tcgaatccag ctaccccagt tattattttt
     1261 aaatttgccg gcgtggcgga attggcagac gcgctggact caaaatccag tgtccgttga
     1321 ggacgtgccg gttcgacccc ggccgccggt ataattatca atatagcaac tttcttatag
     1381 agagttgcta tatttttttt gtttttatgt aattacctcc aaatcaattc gatacaagta
     1441 ttatcataaa acgagtaaaa aagatattga taacgctttt attatgtgtt aaactatttt
     1501 tgtaaattat gtagggtgct actattcaat taggcacgac ctaagaaagg gttctttctt
     1561 aggtcgtttt ttgtctttaa aaattgtttg gagacaaaaa tatgtatgaa ggagtctgca
     1621 agttgttttg ttttatcata tagtggaggt gaattaacgg tgtatgtgat gaaaaagctg
     1681 aatcataatg ctgtattgat cgatgacgat ggaatagaat aaattgcgat ggggaaaggg
     1741 attggctttt ctgctaaagt gagtaagcca tttgatgttg aggatgctga caaattattt
     1801 gtattagatt ctaaagaaaa gatcaaacga ttcagtgaga tagtctcgca gattccaatc
     1861 gaatttatgg attttgcgga agaagtgatt gaactgataa cagctaggat ctcctctcca
     1921 ttagattcaa atatctatat tacattgaca gatcatattt attttgctat tcaacgaaaa
     1981 agagaagata gagatgtcac agcaattatg ttgccagaaa tgagattcct ttatccaaat
     2041 gaatatgata tagcaataga agtggtcgag cgaatcaatc atcgatatca aaccgagtta
     2101 gggtcaaatg aagtaggatt catcactatg catatcatta atgctgagct aggagagcct
     2161 aatagtttaa agagcttaaa aatagtagaa atgacgacca cgattttgtc ccatctagaa
     2221 aaatcctatt caaacgttta tgataaagaa tcactgactt atcatcgctt aatggttcat
     2281 atcaaattcc tggttaaacg cttgatttat caggaagaaa cgccagtaga aaatattggt
     2341 ttttttactg aaacatttaa acatagcaaa gcgtattgta cagcactaaa gattaaagat
     2401 atcatcgaac agcaatttca acttgccatt caagaaaatg aaatcatcta tttggcaatt
     2461 catctagcac gaatctatta aaataagggc gtaactatct ataggcgcaa ccaaatgaca
     2521 catagtaaat ggactatgat tatttggttg cgcctatttt agtagaaaaa agaaggagaa
     2581 tgaaatggga aaagtgattt ttaattcgga tgattttggc tatagccatg gtgtgaatta
     2641 tgggattatg gatgcttatc agcgaggaat tttgacatca actacattga tggcaaatat
     2701 gccgggtttc gagcacgctg tgaaactaag aaaggaaatg cctaggctag gtgtaggagt
     2761 ccatttaaca ttgacgtgtg gcaaaccact tctcaaaact gtcgatactc ttatcgaaag
     2821 cggagagttc agatccttat cttattatca gcatccattt aacattgaca aagatcaact
     2881 gtatcaggaa tggaatgctc agattcaaaa aatttatcat gcggggatca taccaactca
     2941 tttagacagt catcatcata ctcatacttt ggatgaaaac caggaggtag ttgttacatt
     3001 agcaaaaaaa tatgatttgc cggttcggag caactttgaa cgaaaagatg aagttcgtca
     3061 tgtcgattat tttgagcctt gtttcgatgt cgtaggagaa actgatgaaa aaaaacgaca
     3121 aatcaatacg agtgtggaag aatatttggc aagtttactc atgatattga gagaagataa
     3181 aacgatcgaa gtcatgtgtc acgcagcgta tttggatcag gaattgttaa atggttcaag
     3241 ttttgtgtat cctcgtactc accaagtcga atttttaatt cattcagcat ttgctcaaaa
     3301 agtgaaaaat gatcctttga ttgagttagt aacatatgag gatatttaat agaggaggaa
     3361 taaaaatgaa tgttaaatca aatgtgcaga agcttggaag agcgatgcta cttccagttg
     3421 cagcgatgcc attagcggga ttgatcatgc gtttatcagc tgatgatatg ctgaacatcc
     3481 cagtaattgg tgcagctgga aatgccgttt ttggaaactt ggatatctta tttgctatag
     3541 gagttgcaat cggttttgtc aaaaataagg acaaaggaat acctgcactt actggttttt
     3601 tagcgattgc taccttgaaa aaaggcttag aaatcatgga cccagaagtg aatatgggga
     3661 tttttggtgg aattatttcc ggtttagtag cggcatggac gtataatcgc tttaaagaac
     3721 aaaaattacc agtagttttt tcttattttg cgggcgaaaa attcccattg actatggtga
     3781 tgattctaca aacaatcgca gctatttttt tgggctttat ctggccgctc atccaaattg
     3841 ggattgataa ttttgcaaaa ctgctcgttg atatgggggc attaggtgtg gggatattta
     3901 tgtttttgaa tcgtttgctg atcccctttg gactgcacca tgtgttgaat acgtatattt
     3961 attacgattt aggaagctat acatctccta gcaaggaagt tttcagagga gagatgacac
     4021 gctttatcaa cggagatcct caggctggac tgtttttatc aggttttttt gtagtaatga
     4081 tgtttggtat accagctata tgtctagcta tctataaagc agcttataaa gaaaataaag
     4141 agatggtcaa aggaatcatg ggaagcggtg caggaacttc atttattgca aatatcactg
     4201 agcctgttga atttagtttc atgtttcttt ctcctatgtt gtacgtgatc catgcacttt
     4261 ttgccggttt agcagggatt gtctgttact tattcaatat acgaattggt tttacattcg
     4321 gagcatgtat tgttgattat ctcatcaatt ttcgaatcgc taccaatgct attttgattc
     4381 ttccaatcgg aattttcttt tttgccttgt attacgttac tttttattat ttgatcaata
     4441 agagaaacat tcagacatta ggacgtgaag ctaaggcaga gtttggcaat gaagtaacct
     4501 tagaagaaac agagttaggg ttagcaagta aaaattatga atatatggca acaaaaatgc
     4561 tacaagcgtt tggaggaaaa gccaatatcc tggatgtcta tagttgtaat acacgcctac
     4621 gggtagaggt agtggatccc accatggtcg aggaacagcg aatcaagcaa ttagggataa
     4681 gcgggatcat taaaccaact gaaaaaaatt atcaaattat catcggctta gaagtgactt
     4741 atgttatggc agaattcaat aaattattag aagaatagaa aggaaaagat gatgatgaaa
     4801 aaacagatta tcactatagc tggaggcggg agtacgtata caccaggaat cgttcaagcc
     4861 attttgaaca atgaaaaacg gttgccatta tctgaaatac gtttatatga tattgatgaa
     4921 gaaagaaata tggatatgta cttgattgtt aaatttatgc tgaaaaaaaa ggtttttcaa
     4981 atattcgcat tcgtgcaacg gatgatccaa agcttgcttt tacaggatgc gattttgttt
     5041 tctcacaaat tcgtgttgga ggattggaga tgcgagaaaa ggacgaaaag attccactta
     5101 aacatggatt agtaggacag gaaacgtgtg gactcggagg atttgcttat gggatgcgct
     5161 caatcaaagg tttattggaa attgtcgatc atatacaaga ctatgccccc aaagcatgga
     5221 ttttgaatta tacaaatcca gagtcgatcg tttctgaagc tgttcgtcgt aaatatccga
     5281 atgtaaaaat gattaatgcc tgtgatatga ctatttcaat tgaagaaacg attgcagtga
     5341 attacggtta tgatcgtaag aattggatcg ttacgtatta tggtttaaac cactttggct
     5401 ggtatacatc gatttatgat aaagaattgc aacgagaaat tatgccagag attattgaaa
     5461 aactaataac aaaagaaatg caagtcgccg attttaatat aggagacaaa acttggcaaa
     5521 aaacctttca aatgatgtca gtcatcacga aaaattttcc aagtaatatc ccgaataatt
     5581 acttagagta ttacttatat cctgatatgg tcgttgagca tactgacaaa gagtatacac
     5641 gtgctaatat ggtcatggat ggacgagaga aaaataccaa agaaatggct gataaaatac
     5701 gccgcggtat tgtggaagaa gtattgaact ttaattttgg ggagcatgga caatatatcg
     5761 tcgatatagc tatttccttg ttgaatgatg atcgtcgtcg ctttatgcta atcgttccta
     5821 atcaaggggc gattccgaat ctacggtcag atgcagttgt tgaaataccg gcatatgttg
     5881 gtgcaactgg tgtggagaca attacactaa gaaaaccaat cagtgatttt cataaaggat
     5941 tgatggaggc acaagttgca gcagagaagt tactagtaga tgcttacttt gaaggttctt
     6001 atcaaaaagc tttacaagca tttacattga atcaaaccgt accaaatgcc agagtcgcaa
     6061 aaaaaatact agatgaaatg atcaaagtaa acaaaaaata ttggccagca ttaaagtaga
     6121 ggtgataaaa agtgcatttc tttaaaacaa gaaacattct aaaagctgta gctagtggtc
     6181 aaattattcc attaacagca gtaaaagacg aggtcttttc ttcaaaaatg ataggagttg
     6241 gatacgctat aacgaatcat gatggatata tccatgcgcc agttaaagga ataatcgata
     6301 atatatttcc tacccgacat gccataacga tcgaaagtga atctggcaga aaaatactga
     6361 tccatatggg tttagataca gttgaactcg aaggaagtcc gtttcagata cgggttgaaa
     6421 gtaaccagga ggtaaaagaa gggacactgt tagctattat agatttgaag aagttggagc
     6481 aagcacaaaa agatacggtg atcattgtag tttctccagg catttttgaa ggaaatttat
     6541 taaaacaaaa ccaagaagta aagattaaag atagttgttt tgaattttag tgtacgatga
     6601 aaaaagttaa atagggtgat atttatgggt gaatttacaa aatttttttg aaatgataag
     6661 tttcaatcac tagatttaat cattttgatt tttgttaatt atcgaatttt tttgttgctc
     6721 ctatgattta gaaaacttat tttcaggatt cttaaaaata aatttgatta tttaagagaa
     6781 tatattgaaa taaaatttct taattatata attagttatg aacattcttt gcgttgtcat
     6841 gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag ctgatgaaat
     6901 gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt catttattag
     6961 caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca ttttttatgg
     7021 tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat tcggcaacga
     7081 ttgttttacc ttttaggatg acgatgggat tgatggctat ttatgcaact tttgttatgg
     7141 gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata ttatctttag
     7201 gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaaaag gcacctcttg
     7261 gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg atcttatcta
     7321 tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact atcaaaatgc
     7381 cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca ggtgcagtca
     7441 ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac gctaccttat
     7501 tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt gttttactac
     7561 caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt attgtagggg
     7621 ctgttgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg
     7681 gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa tggacggtaa
     7741 caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc ttctgtaaat
     7801 caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc aatatcaatg
     7861 agcccatgat ttttggagct cctatgatgt tgaatcctta catgttcatc ccgttcaact
     7921 tagttccttt agttttgact attatcagtt attttgcagt caaattagaa atggtcaata
     7981 gatttactgt tttgccaact tggacattac cagctccaat cggcggttat ctttcagcag
     8041 gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca tttattatct
     8101 attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa agtcgtggag
     8161 aaaacgaagt ccaaactgtc taagtttggt ttttagaagg tgggaaacaa tgtattggct
     8221 gattttattt tttgttttta tctttttatt aacggctagt caccttatat tgaatatgct
     8281 agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat ttttgatcgt
     8341 gattcttcca aagatcattg taccgcatat gaatgttttg ttttcatggg ggacatatgt
     8401 actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt ttgtcacatc
     8461 gaagctttaa atgttctaaa agtggttgtg acagaagtgg ttagcttcaa gaaataagaa
     8521 gaaatttacg aaaattgctt cttaaatttt tgtgaaatgt tggcttattt ttcgtttggt
     8581 tgaacttatt accgcagatg caagcttttg accaccgttt attcggaata tgggactgtg
     8641 acaaggcttt tgtcacagcc cttttttaca taaagattat tattgtttta actgttttgg
     8701 cagaaagaag gaggcaaaaa tgaaaatttt atcagcaatg gattaagaat tagtcgatct
     8761 actaccaaag tgatcaaggc aaatatagat ttgggagctt acaaccatat agtaggggct
     8821 gcagtatggt gtatgaatgg taatatgtat gatgagagtg tttattcaaa tcatgaaacg
     8881 tgtatagaaa taattgcgct gggaatagca atcagtcatg gtgaacgtga cttttagttt
     8941 atcgttgctg acgacataga acattctgac atgatctact cagcatgtgg ggattgctgg
     9001 cagtttatat aggagtatgc atcggattgc gagatcattg tctcaacaga agagggaggt
     9061 tgattatcaa agataaagtg gtaaatccta catttgattt agtaattgtt gaagggacta
     9121 gcagcacaaa tctctacaaa gcgatgcttc tagatctata tgacacattt taagaaaatg
     9181 tatttgccta attattatgg tctttctttt gaagaaacgt tgagatgatc caaccagaga
     9241 aaggtgtcaa agaatttgac taagaataaa tggcagacgc tacctatgct catttggatt
     9301 gcgtcagatc ttttatttta aggatgattt tgtgtttttt agacaccgta atttaagtaa
     9361 ctctaacttt tcaatccact atttactttt tttgtatttt cactgtctgg gtctcgaata
     9421 gcgaaattac tgatgagtct ctattaaaaa agctttttct acttgaaact atattgggtg
     9481 gttgctcagg aaggtttcga tttcttgaat tgcttgttgt ggaccattta ttggaaaaac
     9541 ggcttgcagc gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc gcagagcaat
     9601 ctgcagtttt ctttcagtca ctggttttgt gagggcgatt gcgtttccgt attttacaca
     9661 atagtcaata ttacagcttt gctcaaattg catccccaca ccgataaacg gtttgccgga
     9721 ttcacaggca gtttggacgg tcccttcccc gccgtggata aaagaaaaat caatgaattc
     9781 atgcaaaaga tgactagaca gatattctgt tattaaaaga tcttctgagt cagattgaat
     9841 gtcatcaagc agatagtcaa taggtgcaat aaccgctaga tccggttgtt tttgtaagaa
     9901 gaacaaaatt ttgcgtaaca tacgcggatt gcctgaacta cccattgcga aatagataat
     9961 acgttttctt tcttgttgct ttcgctgtat aaaagttagc acttcttcag gcaaggaagt
    10021 actgagctta gcaaaaattg gaccaacgat tcgataattt tccggcagct gttcttcttc
    10081 caaaaatagt ttaggcgttg tgatgagatt tagatcagca tccatcattt gtaaacttgt
    10141 ttttggtaaa ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg ggatccatcg
    10201 ggtatttact atacgctttt ttgtccagtg ccaaagggga ttgcttaaag ggccgaagaa
    10261 acgtaactcg tggggcatca gcgcatgctc tgattgaaaa aaaggttcac tcaaagcata
    10321 gggtttaaca tatactaaag gaatcttttt attgcgggcc gataaaaaaa ttgtcacatt
    10381 cgaacctgtc acaatcagtc gaggctcaaa cttagcaatc agactctgtt cactagtgat
    10441 acgagaggca accatttctt tggtaaaagg gtttttaagt gtacgcaact ggtcaaattt
    10501 tatgatttgt tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat actcaaagcc
    10561 actttctgtg atcaaaggag caaaagtttt agaaaagcca aagaatacac agtaataacg
    10621 gtctttcata gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat tgaatactgc
    10681 aggagcaaaa agaattgtct tcatgaaatc ctccttattg gaatgagtct atcccttcgt
    10741 ttcttactag tatactaaaa aacacagcgt tcgagtagcc tatgtatatt ttgaatatta
    10801 aactgtctct cgctgttttc acaacttcta aatttaaatt ttgtaatcag gttgaaactc
    10861 caattttcaa aaaaacacgc aacaattttt tgatcgattt atatgttgtg ccagataaat
    10921 acgagaagtt gtgtcattaa aaataaacat ga
//
LOCUS       DACDNO010000077        10734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDNO010000077 DACDNO010000000
VERSION     DACDNO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.077"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWI22_002382"
     CDS             6..305
                     /locus_tag="IWI22_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9169735.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWI22_002383"
     CDS             378..1739
                     /locus_tag="IWI22_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9169736.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWI22_002384"
     CDS             1761..2021
                     /locus_tag="IWI22_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169737.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWI22_002385"
     CDS             2035..2787
                     /locus_tag="IWI22_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9169738.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWI22_002386"
     CDS             complement(2881..3486)
                     /locus_tag="IWI22_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9169739.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWI22_002387"
     CDS             complement(3539..4513)
                     /locus_tag="IWI22_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9169740.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWI22_002388"
     CDS             complement(4891..5670)
                     /locus_tag="IWI22_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9169741.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWI22_002389"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWI22_002389"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9169742.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWI22_002390"
     CDS             7161..8558
                     /locus_tag="IWI22_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9169743.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWI22_002391"
     CDS             8639..9079
                     /locus_tag="IWI22_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9169744.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWI22_002392"
     CDS             9227..9553
                     /locus_tag="IWI22_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9169745.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWI22_002393"
     CDS             9554..10303
                     /locus_tag="IWI22_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169746.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELIGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..>10734)
                     /locus_tag="IWI22_002394"
     CDS             complement(10383..>10734)
                     /locus_tag="IWI22_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9169747.1"
                     /translation="PLTRKEIFYLLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSEL
                     VDLIVSHFTLLQRPIITDGKKLLVGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLE
                     NSTDLDDEFLNYYI"
BASE COUNT         3406 a         1892 c         2208 g         3228 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg ataggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggc
//
LOCUS       DACDNO010000078        10693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDNO010000078 DACDNO010000000
VERSION     DACDNO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.078"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..450)
                     /locus_tag="IWI22_002395"
     CDS             complement(<1..450)
                     /locus_tag="IWI22_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9169748.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IWI22_002396"
     CDS             674..1465
                     /locus_tag="IWI22_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9169749.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IWI22_002397"
     CDS             1431..2384
                     /locus_tag="IWI22_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP9169750.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IWI22_002398"
     CDS             complement(2782..3756)
                     /locus_tag="IWI22_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9169751.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YNAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IWI22_002399"
     CDS             3871..4305
                     /locus_tag="IWI22_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9169752.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IWI22_002400"
     CDS             complement(4430..5416)
                     /locus_tag="IWI22_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP9169753.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..6266
                     /gene="arsC"
                     /locus_tag="IWI22_002401"
     CDS             5844..6266
                     /gene="arsC"
                     /locus_tag="IWI22_002401"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP9169754.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6546..8468)
                     /locus_tag="IWI22_002402"
     CDS             complement(6546..8468)
                     /locus_tag="IWI22_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9169755.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8555..10495)
                     /locus_tag="IWI22_002403"
     CDS             complement(8555..10495)
                     /locus_tag="IWI22_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9169756.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
BASE COUNT         3275 a         2088 c         1826 g         3504 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatta tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag
     6001 aagacggcat cgatatttcc aaacagacgt ctgatttgat tgatagagat tactttactc
     6061 aggcagactt gatcattaca ctttgtggtg atgccaaaga caagtgccct gccgttccaa
     6121 acaatcacga acaccttcac tgggacctaa aagatcctgc acaagctgtc gggagcgaag
     6181 aagaaatatt aacggaattc agaaaagtca gagaaggaat caaaagaaaa gttttagact
     6241 taaatcaacg tacatcgatc gtataaagaa gtattcaaaa tgatttttca agcactaaga
     6301 aacaatgtta aaaaaatagc tttccatatt ttacaaaatt gtgccttatt gccgacaaat
     6361 tgtctcttga acaccgttta ccagtagtca agacatcgta agcaactata aaaaattata
     6421 agaaactcaa aataaaagga tgtgccacaa ctttggccac atcctctttt tttgtattta
     6481 gttttctact tccaaataaa tagatagaat tattataaat ataaaggcag ttttttaatc
     6541 gctacttatt cataactaga taacaacgtt aagatttcta gcggatgatt ggattgcatc
     6601 aactgagcca tttgttctgg ctgcttttct agatagtgaa taacagaaaa tatttttttg
     6661 aactctgcat gtttcaaatc atttttcttt attgctaata gcagtacgat acgtatctgt
     6721 tccccgtgcc ataggactgg ttctttcatt gtagcaatag aaatcatcga atgcttcaca
     6781 tacagcggat taccgtgagg gattgttaca aatggtgcta ctctagtact ggagtgactg
     6841 tctctttcca agacagaatc gatgaattcc ggctccacat aatgatgttc cactaagacg
     6901 tttcccataa agtttaaaat atcggctggg tctgtccgtt caagttgagg aaagatcaga
     6961 aatggatgag taaagcttgc tagatcaaat gtttttttct tcttgatagg cacatgctcc
     7021 agcatatatt ttgtcaaata ggctagatca gtttctgtga tcattggtga gatctcgatt
     7081 actggaatgc ttatatgttt aaatggtaac gtagccaaga taaaatcaac agagggaaaa
     7141 tcttgtctct ttaattctgg cttatattca actagatcca taatcgttaa ttctggaagt
     7201 tctctgttca gccgtgcttc gagaaaagta gccatagctt ttgaatattc actaactaaa
     7261 accgtttgat atcttctact tttctgatgt ttgtgtcgct cgatcgctgc ttggaaatgg
     7321 atcgttaaat aagctgtctc ctcttgtgga acataaaaat tttccttttc aaaataatct
     7381 tctaaaatta actgtaaaac taagaaaagt tgtgtgtacg tactaaacac atttttcgtc
     7441 aatggattag aaatctgcaa accataatgg agtcgaaaat aagttgaaga taaatgagac
     7501 cttaaatttt gttttagtag ctgatcttct tttaatgaat agcctagaaa ctgttccact
     7561 tgttcgatca aatgatcagc aagtgcgcta gattcctttt gaaaacgtgt ggctgcttgt
     7621 gaatttgtaa aaagattggc gatacgcaaa gccagataat tgatttcatc aggagaaaat
     7681 tcaattggat agatggtggc tagttttgct aatatttgac tacttaactt ttgtgcttgg
     7741 gtgtgttcca cgacttcgtt ttcgtcttta cttaatttca ctttctggta ctgtttcatt
     7801 cgctcgagca taaagtagat gtgaatctcc acactagcca gtccattcgg ctgttctaaa
     7861 tggttttcat aaaaaacatc ttgtaatgta tgttggataa cttttaatgt atctttagcg
     7921 aagaattctt tcaacgacgg actttgtgga tcttctttct gcatatttcg taatgtctta
     7981 acaagcaact gtcttttttc ttgttcatcg cctttaagaa aagttcctag ttttgttttg
     8041 gatactagct gtaaatggtg atactcaaaa aagctttgca actctgtcaa ttctttacga
     8101 atcgtacgtt ttccaacata tagtttttct gaaagttctt caagactaaa tccgtctttt
     8161 gctactagca gatggaaaat caataactga cgaagctgtt ttttatccat ttctgtttga
     8221 gtctcttcat taagcgctcg tttcagttgt tccctttcgt gaggcagacc attcaacaag
     8281 atacctgtac ctttttttct ttcaaacatt aaaggcagat gtttgtctct caaccaagtt
     8341 tcaatggtat tcaaatcgct atgaatcgtt ctttctgaca catggaaatg ctctgctagc
     8401 ttttttgttg ttatataatt attgttctca cttaattgat agatcaaaga ttgctgacgc
     8461 tgattcataa tgtgcagaaa ctcccttcta aataaaaaat cgatcatgat aacacatcta
     8521 ttatgtctca tcacggtcga tttttcatct tatgttacat ttcgcttaat aattggtgaa
     8581 ctgttgtttt atctggcgct tccatcagtg cttgacggaa gtcatcatcc atcaatttac
     8641 gtgatagacg ttgcagtatt ttcaagtgca tatcaccttg gctgttttca ggtacagcaa
     8701 tcataaagat catatgtgca ggttttccat ctaaagaatg ccaatctacc ccagattgac
     8761 ttctggcaaa tgccactgtt ggtttaagaa cataagctga ttttccatgt ggaatagcaa
     8821 tccccatacc gattcccgtt gtactttcat gttctctatc atagatgact tctttaaatc
     8881 tggttttatc tgtgatcgcc gctgtttttt ctaaacgttg gactaaatct tcgattactc
     8941 cgtcttttgt tgttgctgtt tcacctaaaa cgattgcttc atctgacata atatccgtga
     9001 tagaatcaaa agtttcagtt ttcgtataaa catcacttgt ttcagtctct gatagatctg
     9061 ctactgtcac accatttgct gcagcaaccg cagtttgttg taatgtcggt ttaaggattc
     9121 ttaacattag cacggttaca gcaataccag caatcactgc tacaaaaaac atggctacgt
     9181 gatccactgc tcctaaaacc gctacgatcg gaccgccatg agctacatga tctgtcactc
     9241 ctgaaagcat cgcgatcaca cttcctgtca ttgccccgat tacaatgctt ggaatcaccc
     9301 gtagtggatc tgttgaagca aacggaatag cgccttctgt aatcccaaac agtcccattg
     9361 ctaaggacgc cttaccagct tctcgttctg ttttcgtaaa tttggttttt aaaatcaatg
     9421 atgccagccc caatgctaat ggagggatac agatagctac agcaattggc cccataatgg
     9481 aataattgcc ttctgcaatc aagccagatc cgaacaaaaa ggctacttta ttgaacggcc
     9541 cccccatatc aaaagcaatc atcgctccta agatcatagc tagaacaatt tcacttcctc
     9601 cttgcatccc tgctaaaaag tgagtcaatg aactaaatag aagagctact ggttctccaa
     9661 taacatagat aaacacaaaa ccaactgcca atgatcctaa aattgggata acaataattg
     9721 gcatgatcga ttgtaaagct ttcggtactc gcactttctt gattcctaaa gcaatgaatc
     9781 ctgctaaaaa tccagtgatg atcccgccta agaatcccgc attcgcatca ctaccatata
     9841 atgctccgtt tgccgctaac atcccaccaa caaatccagg aacaagtcct ggacgatctg
     9901 caatactttg cgcaataaat gcagatagaa ttggaaccat caaccccata gcgatacttc
     9961 cgatactatt catcgttgcc caaatcgttc cttctggtat cttgatccct tcaggtgtag
    10021 ctgttcctcc taatgttaaa gaaatcgcga tcaataaccc tcctgtaact acgaaaggta
    10081 ccatgtacga aacgccattc atcaaactct tatagatttt tttacctaaa ttttctgatt
    10141 cacttttatt ttcggaagaa ataattgcct ccgtactttt atagactggc gcttttcctg
    10201 ctaaaatatc tctcaccaac tcatctgcat ggtggattcc ttcttgcaca cctacggtta
    10261 tcaaaggttt accgccgaat cgtgatttat ctattttagt atctgctgcg atgattacac
    10321 cagctgcttc ttcgatatct cttgcagtca attcattttc aataccgatg gaaccatgtg
    10381 tttcgacttt tacctcggcg ccaactgctt tccccgcttt ctctaagttt tctgctgcca
    10441 tgtatgtatg ggcaatacca actggacagg atgtgattgc aataattttt ttcattttcc
    10501 taccgccttt ctacatttat agtatataaa aacggtttct ttttattaag cacaaaatga
    10561 ttccgtaata actgaaattc ttggaataaa tagaaatttc gctaaaaaaa ttgatatacg
    10621 atttattatg ttgaataaaa taatattgga aaatcttaca tttctgtact tctgcttacg
    10681 tacatctaaa tta
//
LOCUS       DACDNO010000079        10495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDNO010000079 DACDNO010000000
VERSION     DACDNO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.079"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..359)
                     /locus_tag="IWI22_002404"
     CDS             complement(<1..359)
                     /locus_tag="IWI22_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9169757.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
     gene            complement(711..1070)
                     /gene="rplT"
                     /locus_tag="IWI22_002405"
     CDS             complement(711..1070)
                     /gene="rplT"
                     /locus_tag="IWI22_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9169758.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1123..1323)
                     /gene="rpmI"
                     /locus_tag="IWI22_002406"
     CDS             complement(1123..1323)
                     /gene="rpmI"
                     /locus_tag="IWI22_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9169759.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1395..1919)
                     /gene="infC"
                     /locus_tag="IWI22_002407"
     CDS             complement(1395..1919)
                     /gene="infC"
                     /locus_tag="IWI22_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9169760.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2239..2871
                     /locus_tag="IWI22_002408"
     CDS             2239..2871
                     /locus_tag="IWI22_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9169761.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2873..3778)
                     /locus_tag="IWI22_002409"
     CDS             complement(2873..3778)
                     /locus_tag="IWI22_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9169762.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3863..4516)
                     /locus_tag="IWI22_002410"
     CDS             complement(3863..4516)
                     /locus_tag="IWI22_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9169763.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4838..5782
                     /gene="mvk"
                     /locus_tag="IWI22_002411"
     CDS             4838..5782
                     /gene="mvk"
                     /locus_tag="IWI22_002411"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9169764.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5775..6752
                     /gene="mvaD"
                     /locus_tag="IWI22_002412"
     CDS             5775..6752
                     /gene="mvaD"
                     /locus_tag="IWI22_002412"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9169765.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6765..7850
                     /locus_tag="IWI22_002413"
     CDS             6765..7850
                     /locus_tag="IWI22_002413"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9169766.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7847..8911
                     /locus_tag="IWI22_002414"
     CDS             7847..8911
                     /locus_tag="IWI22_002414"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9169767.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9160..9963)
                     /locus_tag="IWI22_002415"
     CDS             complement(9160..9963)
                     /locus_tag="IWI22_002415"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9169768.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9983..10288)
                     /gene="kduI"
                     /locus_tag="IWI22_002416"
                     /pseudo
     CDS             complement(9983..10288)
                     /gene="kduI"
                     /locus_tag="IWI22_002416"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10270..>10495)
                     /locus_tag="IWI22_002417"
     CDS             complement(10270..>10495)
                     /locus_tag="IWI22_002417"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP9169769.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3105 a         2220 c         2008 g         3162 t
ORIGIN      
        1 atacctaatg acatcaagat cggcaacaca ataactgcaa tagaaatcac tggtccgatt
       61 cctgtcatcg acatgaatat aacagcggtt acaatacata gtaagctcat cgtgattctt
      121 ggcttgtccc ctccaagttc aactaccttt cgaatcaggg tagctgcgat tccgctatct
      181 actagaacac gcccgaaaaa ggcaccaaag aagacattta ccaagatcga tttggcgtac
      241 tcagctggcc ccgtcgtata gatatgcgtc aacacatcaa ttactccttg cccttccatc
      301 gccgagtttg gtgaaaacgt gtttccgact agcgctaatc ccatccatac agtagccatg
      361 atgacaaaag ccgaccatca agttgtagcc ttttacacag taccagacca tgaaaaagaa
      421 tgagataact aacaaaatac caataataat atcactcatg ttattcctcc gtccattttg
      481 tttttctgat aagcaaacta atatatcagt caaactcatt ctaacttatg tagttagcgc
      541 tttcattaaa ggatgaccat aaatgttcag ttttcaatta aaaaacctta attatgttat
      601 tttagactta tacgttattg tcctgtttca tttgatggag gattgtaaat aaaaaaagag
      661 gaaagaacat aacttttcgt tatcttcttt cctctttata attgtttagc ttatttagcc
      721 aaagcttctt ttgcttggtc agccaatgca gtaaatgctg ctgcatcgtt aacagccaag
      781 tcagctaaca ttttacggtt gatgtcgatt tcagcaactt tcaaaccgtg cattaatttt
      841 gagtagctca agccattcat acgagctgct gcgttgatac gagcaatcca caatttacgg
      901 aagtcgcgtt tcttttgacg acgatcgcga tatgcgtagt tgtatgaatt cattacttgt
      961 tcttttgctg ttttaaataa agtgtgtttt gaaccgtagt aaccttttgc taattttagc
     1021 actcttttac gacgtttacg agttactgtt ccacctttaa cacgtgccat gtataattcc
     1081 tcctaataaa aataaatctt cagattctgt tatttgtact tcttatttca tttttgctag
     1141 ttgttggcga atacgtttga agtcgccact agaaaccatg cttgctttac gcaattgacg
     1201 gcgttgtttt tttgttttgc cgtggaaacg gtgacttgta aacgcgcgga agcgtttcaa
     1261 tccgccttta ccagtacgtt tgaaacgttt tgctgatccg cggtgtgttt tttgttttgg
     1321 catgactata tttcctcctc aaaatctttc gttatccgac tatcggctgt ccaacagcca
     1381 gttagtcatt caaattactt actgtcgttt ttcggtgcca gcgtcaagaa catgctgcgt
     1441 ccgtccattt tcgctttttg ttccactgtt gcaatatccg cagtttcttc agctaagcga
     1501 ttcaagactt tttgaccaat ttctttatgg gtaatggcac ggcctttgaa acggatagaa
     1561 gctttcactt tatcgccctt ttccaagaac ttacgtgcat tacgaagttt tgtattgaag
     1621 tcattcaagt cgatcgtagg acttaagcga acttctttta cattgatcac tttttgtttt
     1681 ttacgcgctt cgcggtcttt cttttgttgt tcgaaacggt atttaccgta gtccatgatt
     1741 cgcgcaactg gtggtttcgc accaggggca actaacacaa gatccaagtt tgcttgttct
     1801 gcaatattca atgcttctgc ttttgtctta actcctaatt gctcaccatc tgaaccgatc
     1861 aatcgtaact ctcgtgcacg aatgccgtcg ttaaccatca tatcctttgc tatggtcatt
     1921 cacctccaaa tattatgaga gaaagaaaag cttaccgaat aaacggcaat aaaaaaacga
     1981 gttctcttac agaacccgcg cgtacttcac ctaatagaac cagtgaaaac tatctagccc
     2041 agtgacatta tcaactaagg cgagaagcgg gtgcttctgc ttttatttct caaccattat
     2101 agtatagcaa aaactaatct ttttgtcaat gtttttcata cagtttgtct tccttttctg
     2161 aaaatgatta gattagcagg ctttctagca gtgattcccg cttctttgct atactaaaga
     2221 aaaacgaggt gaacccacat gaaaaaaatc gaacctattc caccttctct tcctcttctt
     2281 acaaagggag agttttattt agaagatgag gagatcttct ctggtcttct gatcaacgga
     2341 ggtaaccaaa gctatctcga tgccaaaaat atggtattaa gagagagtca tatccaaaaa
     2401 cttacgatgc aaaaaacagg tttacgtcat tttgaatgca gtaatgtcat ttttgaaaaa
     2461 tgtgattttt ccaatctgga gtggattggt ggcagtttcc accaagtcgt gtttcatcaa
     2521 tgtaaattaa caggtacgaa ttttgccgaa agttatcttc gagactgtac tttcactgat
     2581 tgtattgctt cgatggcttc ctttagtagt acaaacttga aagctgtctc cttcgatcat
     2641 tgtcaattag aagacagcga attttatgag gtcacttgga aaaacctttt tttgtctgac
     2701 aatcagttaa ccggttcgaa ctggtttcga acttctctga agggattaga ctttacgact
     2761 aatttcttca caacaatcgc tttatctcaa gattctttgg cgggactaat cgtcaatcaa
     2821 gagcaagcac ttgtgatcgc ccaagccctt ggtttgcaga tcaaagacta gatcaacgaa
     2881 tccgccgcca cagtgcaata agcatccaaa tagataagac caaactgatg atcaaggcga
     2941 tttcccatcc gaaaacggaa tgggtaaagg gtaactggag attcattccg aagaatcctg
     3001 tcacaatagt tggaacggtc aagatcagcg accagaccgt caaaaatttc atcgtgtcat
     3061 tcaagttgtt attcagcaaa ttattataag tgccagagag ctggtctaaa atctgcgaag
     3121 ctaagttcgt catttcaact gcttgtcgtg cttcgatcag tgcatcgtcc aactcttctt
     3181 tttccttttc tgacaacttg cgataaatag ccagtgcctt gatttgttct aaaagtacta
     3241 cattttgttt tgttcctgta actaagtaaa ccaaaccgat ctctaaatct gaaagttgca
     3301 gcagattctt gttcgttgtt ttttctctta atttcagatt aagccgctga cgttcactat
     3361 tcacttcctc gattaaagga aaaaaggaat cagaaatcaa aaacaatgta cgaaacaata
     3421 aactgtatac actcatgtct ggcatccgtt ccaaaagcga tcgaattagt cgaatcacgt
     3481 actcggtatt cggcgtggta aatgtaaaca aaccgtcttc ttttaaaata aaagcgatag
     3541 gactagtttc ataatgatta tcgacttttt gctggtgcgg aacatttgat acaagcagta
     3601 acgcttcatc aaaagaatcg tactctacgc gcgcccgttc atttttatct agagaataag
     3661 tcagcatctc gtcactaatg ccatattctt cttttaagtt tttaacagct agcacatcgt
     3721 tttgctgtac attgatccaa aaattacggt cttgattaaa atagtgtttt tcccgcatac
     3781 tctatcctcc tgacatttgt ttccagtata aagaaaaaag tcgctcaaat aaaggtttac
     3841 cctttatttg agcaactgta aatcaaatgg atactttggt ttggcgaatc tctgctaatt
     3901 tttcttcgat cattttcaag acttcttctt ttgcttgagg atcttccaca aaattatagc
     3961 ggtctccatc gatttggatt tttggacttt ctttgtattc ttcataccat tgatcataac
     4021 gtctgttcaa ttctttgtaa taatcgtata gacttgggtc aaaggacaat tgttcatacg
     4081 aacgaccacg tttttcgata cgctctaaca tcgtatcaaa agatacacga atatgaacta
     4141 ataaatccgg atgtttttta tgggcagcat agggaagttc ttccatcatg ttttccagca
     4201 gttcatcata cacacaaact tctgtttctg ttgctcttcc tagatctgca tttagatgga
     4261 aaagcagtga atcttcataa atcgaacggt ccaatacatt attgtcatct ttcattgctt
     4321 gtttgatgct tgcaaaacgt ttattcaaaa aataaatttg cagcaaaaaa gcatactgtt
     4381 cagggtttgc ataaaataag ggcaagactt cattatcatc aatcgattca taaaatgcct
     4441 gactatcaaa gtgttcagcc atcatttctg ttaaacttga ttttccggca ccaattgtac
     4501 ctgctaaaac gatcacgctc atttacctcc agctcacttc atcactaaat attgtgttta
     4561 actccaacct tttttaaata tacccccaat atctagtagt gtcaattgta ttttttctag
     4621 tcaaagaaga aaaaaagtca aaaaattctt aagaaaaaag tgttttttct ttagaaaacg
     4681 tattcttagt tttacttctg agatggtttt agtaaaataa aggaggaatg ttttttgaga
     4741 cccttagtgg acaaaaaaat tcgtgaagca gcgcaactcg ttttgggcgc tttatttttt
     4801 gcttaaaaat cacttttacg tgagaaggag ttagattatg gcaaactatg gccaaggaga
     4861 atcaagcgga aagatcatat tgatgggcga gcacgcggtt gtttatggag aaccagcgat
     4921 tgcctttcct ttctatgcaa caaaagtcac cgcattcctt gaagagctgg atgcaatgga
     4981 cgatcaactg gtttcttcct actattcagg aaatttagcc gaagctcctc atgcattaaa
     5041 aaatatcaaa aaattattca ttcacttaaa aaaacagcat gacatccaaa aaaacttgca
     5101 actgaccatt gaaagcacga ttcctgctga acgtggaatg ggatcaagcg ctgcagtcgc
     5161 cacagcagtc actcgtgctt tttatgatta cttagcattt cctttgtctc gtgaaatact
     5221 attagaaaat gtccagcttt cggaaaaaat cgcccacggt aatcctagtg gaatcgatgc
     5281 agccgctact agcagcttgc agccgattta ttttacaaaa gggcatcctt ttgactactt
     5341 ttctttgaac atcgatgctt ttttgattgt cgctgataca ggaatcaaag gacaaacaag
     5401 agaagccgtc aaagatgtcg ctcacctctt tgaaacccag cctcatgaaa ctggacaaat
     5461 gatccaaaaa ttaggatact tgacgaagca agcaaaacaa gcaatcattg agaattcacc
     5521 agaaacgttg gcacagacaa tggatgaatc acagtcactt ctggaaaagc tgacaatcag
     5581 caatgatttt cttaatctat tgatccaaac agcaaaagat accggggcat tgggtgccaa
     5641 attaactggc ggtggacgcg gtggctgtat gattgcatta gcacaaacaa aaacaaaagc
     5701 ccaagaaatc agtcaagcac ttgaagatgc gggtgctgct gaaacttgga tacaaggatt
     5761 aggagtacat acctatgttt aaaggcaaag cacgcgcata taccaacatt gctctaatca
     5821 aatattgggg taagaaaaat gaagaactca tccttccaat gaacaacagc ctttcgttaa
     5881 cattggatgc attttataca gagacagaag tcatcttttc tgatagctat atggtagatg
     5941 aattttatct agatggcacc ttacaagacg aaaaagcaac aaaaaaagtc agtcagtttc
     6001 ttgacctttt tcgtaaagaa gccggcctct cactaaaagc ttcagtaatc agtcagaatt
     6061 tcgttcctac cgcagctgga ttagcctcct ctgccagcgg gctagctgct ttagcaggag
     6121 cttgcaatac tgctcttaag cttggattag acgatctctc tctttcaaga tttgctcgac
     6181 gcgggtctgg ttcagcttgc cgaagtattt tcggtggttt cgttgaatgg gaaaaaggcc
     6241 atgacgactt aagttcttac gctaagccag tcccttccga ttctttcgaa gacgatttag
     6301 caatggtttt cgttttgatc aacgaccaga aaaaagaagt gtccagcaga aatgggatgc
     6361 gtcgaacagt cgaaacatcc agtttttatc aaggctggtt agattccgtt gaaggggatc
     6421 tatatcaatt gaaacaagca atcaaaacaa aagatttcca acttctcgga gaaacgatgg
     6481 aaagaaacgg actaaaaatg catggaacga ctttagctgc ccagcctcca ttcacttact
     6541 ggtctcctga cagcttaaaa gcgatggatg cggtgcggca attgagaaaa aaaggcattc
     6601 catgctactt tacgatggat gctggcccta atgtcaaagt tttagtggaa aacagccatt
     6661 tatctgaggt acaagaaaca tttaccaaat tatttagcaa agaacaagta attactgccc
     6721 atgctggtcc aggaattgcg attattgaat aaagggagat ttttatgatt gaagtatctg
     6781 caccaggcaa actctatatt gccggagaat atgcagttgt tgaaacagga catccagcag
     6841 ttatcgctgc agtcgatcaa ttcgtaacag tcactgtaga atccgcacga aaagtcggaa
     6901 gtatccaatc tgcacaatat agtgggatgc ctgtacgttg gacaagacgc aacggagaat
     6961 tagttttaga tattcgggaa aatccttttc attatatcct tgctgctatt cgtttgactg
     7021 aaaagtatgc acaagaaaaa aacattcttt tatcctttta tgatctgaaa gtaacaagtg
     7081 agttagacag ttcaaatggc cggaaatatg gattgggttc tagcggtgcc gtgaccgttg
     7141 caacggtcaa agccttgaat gttttttacg cgttgaattt atctcaattg gagattttca
     7201 agattgcagc actagccaat ttagcagttc aagataatgg ttcctgcggc gacatcgctg
     7261 ctagctgtta tggtggctgg atcgctttct caaccttcga ccatccttgg ctccaagaac
     7321 aagaaactca gcattctatc agtgagttac ttgccctgga ttggccaggt ctatccattg
     7381 agccattgat tgctcctgaa gatttacgtt tattgattgg ttggacgggt agccctgcct
     7441 ctacttctga tttggtcgat caagttcacc gttcgagaga agacaaaatg gtggcttatc
     7501 agcttttctt aaaaaacagt acagaatgtg tcaacgaaat gatcaaaggg tttaaagaaa
     7561 ataatgtaac gttgatccaa cagatgattc gaaaaaaccg acaattactg catgatttat
     7621 ctgcaatcac tggggtcgtc atcgaaacgc ctgctttgaa caaattgtgt aatttagctg
     7681 aacagtatga aggagccgca aaatcttctg gtgcaggtgg tggcgattgc ggaatcgtaa
     7741 ttgttgacca gaaatctggc atccttcctt taatgagtgc atgggaaaaa gcagaaatca
     7801 ctccactgcc gttacatgtc tatagcgatc aaagaaagga aaaccgatga atcgaaaaga
     7861 cgaacatgtt tcactagcta aagcctttca tgataaacaa aaaaacgaat ttgacttcgt
     7921 acggatcatc cataaccctt tgccgcaaat agcagtttct gatgtcgatc taagtacaca
     7981 ggcagttggg tttacactaa gcagcccatt ttatatcaat gcgatgactg gcggaagcga
     8041 aaaaacaaaa aaaatcaacc aagatcttgc aatcgtcgca agagaagcag atttgatgat
     8101 tgccactggt tcagtcagcg ctgccttgaa agacccttct ttagccgata cttatacgat
     8161 tatgcgacaa gaatatcctc acgggaaaat cattgccaac atcggtgcag ggacttctgt
     8221 cgaaagagcc caagaagcta tccgattgtt tcacgcagat gccttacaga ttcatttgaa
     8281 tgcacctcaa gagttggtta tgcctgaggg agaccgtgat tttaccaatt ggaaagtact
     8341 tattcaagag actcaaacag ccattgacgt ccccctcatc gtaaaagaag tcggttttgg
     8401 catgacaaga gaaacgctca atgatctagc tgctttagga gttcatacag ttgatatcag
     8461 cggtcgaagc ggaacaagct tcacccagat cgaaaacgcc cgtcgctcca agcgggaact
     8521 gagttattta gctgactggg ggcaatcgac agtctcttca ttgcttgaag caaatgaagc
     8581 agacacttct atggaaatcc tagcctccgg aggtatccgc aatgcctatg acatatttaa
     8641 agccctttgt ctcggcgcaa atgcagtggg tacttcagga accgtactga cgcatctgat
     8701 gaatcacggc gtagaagaaa ctattatact gatgaagcaa tggcaagaag agcttcgtct
     8761 gctttatact atggtcggcg caacgaatac cgcggcccta caccaacaat cactgatctt
     8821 ttccggacca gtgaaggatt ggtgcgaagc aagagggatc gacttagtga agtatggaag
     8881 acgcacagaa aagagcgtgg gacaaaagta aagaacactt ttatcccacg ctctaaacac
     8941 gtataaacgg cggaagcaaa agcaactcct tcggaaataa gctgaaattc acaaaaattt
     9001 gaaaagcaat tttcgtgaat tccttcttat ttcttggagt taaacgcttc tgtcccagcc
     9061 tcttttttcg aataaatgat gtttaatcag ctgtccttcg gtattcgttc aagccgctga
     9121 tttcacaacc tctccactaa acggtgtact aaagctcttt taaggttgtt ttccgatata
     9181 ggcaagtatg cctccgtcca catacaagac atggccgtta acgaaatcag atgcgctaga
     9241 agccaagaag acagatggtc cagccaaatc gattggctcg ccccagcgtg ctgctggcgt
     9301 acgtccaacg ataaattgat cgaatggatg acgttcaccg ttttcttgtg tttcgcgtaa
     9361 tggagcagtt tgcggtgtag caatataacc tggtccaatt ccattacatt ggatattgta
     9421 ttgaccgtat tcagaagcga tgtttttcgt cagcattttt aatccgcctt ttgccgcagc
     9481 ataagcactg accgtttcgc gtcctaattc actcatcatg gagcagatat tgatgatttt
     9541 accgccgcct ttttcgatca tatcccgaat aactgctttt gaaacgatga atggggcatt
     9601 taaatccacg tcgattactt ggcggaagtc tgccgcatcc atttcgatca tcggtgtccg
     9661 tttgatgatc cctgcattgt tgactagaat atcaactgaa cctacatctt ctttgatctg
     9721 ttgaaccatc gcttgaaccg cttcttcatc ggtcacatcg cagacataac cttttgcatc
     9781 gatgcctgct tctttataat gcgctatacc ttcatcaact gattcttgat tcaagttatt
     9841 aaatacgatt tttgctcctg ctgtagctaa agagcgtgca atctcaaatc caataccata
     9901 gacagcaccg gtgactaatg ctacttttcc atctaaacga aacatatcca tactaaattc
     9961 catcattttc tctcccttat gcttatttta attgatccat ttgcaccata tccatgtctg
    10021 tataggtgat attttctccg cacattgccc agatgaatga gtaattgctt gtacctacac
    10081 ctgaatggat cgaccagctt ggcgaaataa tggcttgttc attgtctacg actaaatgtt
    10141 ttgtttcatc tggtttgccc atcatatgga acacacgtgt atcttcattg gcataatcaa
    10201 aatagacata agcttccata cgacgttcat gtgtatgaca aggcatcgta ttccaagcac
    10261 ttcctggttc taaaatcgta taacccattt ataaatgaca gctatcgctg aagcttctac
    10321 tgctgtaaat atccccgtca agataccacc gataatgatg acaatcaata gaaggctagg
    10381 aatcgcctct tttattattt ttccagcatg agccatctgt cctttttcga caacagggta
    10441 tttattcttt ttagcaacga tgaatgctac tagcatgatt cctagcgccc acaaa
//
LOCUS       DACDNO010000080        10132 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDNO010000080 DACDNO010000000
VERSION     DACDNO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.080"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            121..627
                     /locus_tag="IWI22_002418"
     CDS             121..627
                     /locus_tag="IWI22_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9169770.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            644..1543
                     /locus_tag="IWI22_002419"
     CDS             644..1543
                     /locus_tag="IWI22_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9169771.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1630..2514
                     /locus_tag="IWI22_002420"
     CDS             1630..2514
                     /locus_tag="IWI22_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9169772.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2706..2777)
                     /locus_tag="IWI22_002421"
     tRNA            complement(2706..2777)
                     /locus_tag="IWI22_002421"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2742..2744),aa:Arg,seq:ccg)
     gene            2837..4171
                     /gene="rpoN"
                     /locus_tag="IWI22_002422"
     CDS             2837..4171
                     /gene="rpoN"
                     /locus_tag="IWI22_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9169773.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4374..5411
                     /locus_tag="IWI22_002423"
     CDS             4374..5411
                     /locus_tag="IWI22_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9169774.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5452..6453
                     /gene="gap"
                     /locus_tag="IWI22_002424"
     CDS             5452..6453
                     /gene="gap"
                     /locus_tag="IWI22_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9169775.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6563..7753
                     /locus_tag="IWI22_002425"
     CDS             6563..7753
                     /locus_tag="IWI22_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9169776.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7857..8612
                     /locus_tag="IWI22_002426"
     CDS             7857..8612
                     /locus_tag="IWI22_002426"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9169777.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8728..10026
                     /gene="eno"
                     /locus_tag="IWI22_002427"
     CDS             8728..10026
                     /gene="eno"
                     /locus_tag="IWI22_002427"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9169778.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3548 a         1869 c         2099 g         2616 t
ORIGIN      
        1 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
       61 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
      121 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
      181 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
      241 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
      301 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
      361 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
      421 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
      481 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
      541 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
      601 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
      661 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
      721 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
      781 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
      841 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
      901 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
      961 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
     1021 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
     1081 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
     1141 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
     1201 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
     1261 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
     1321 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
     1381 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
     1441 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
     1501 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
     1561 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
     1621 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
     1681 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
     1741 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
     1801 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
     1861 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
     1921 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
     1981 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
     2041 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
     2101 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
     2161 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
     2221 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
     2281 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
     2341 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
     2401 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
     2461 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
     2521 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
     2581 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
     2641 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
     2701 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
     2761 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
     2821 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
     2881 gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
     2941 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
     3001 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
     3061 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
     3121 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
     3181 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
     3241 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
     3301 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
     3361 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
     3421 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
     3481 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
     3541 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
     3601 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
     3661 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
     3721 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
     3781 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
     3841 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
     3901 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
     3961 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
     4021 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
     4081 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
     4141 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
     4201 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
     4261 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
     4321 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg
     4381 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
     4441 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
     4501 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
     4561 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
     4621 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
     4681 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
     4741 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
     4801 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
     4861 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
     4921 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
     4981 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
     5041 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
     5101 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
     5161 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
     5221 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
     5281 tcatggcaat cgcgggcgga aaatcaaaag ctaaagcaat tcgagcttat atgaaaaatg
     5341 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
     5401 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
     5461 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
     5521 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
     5581 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
     5641 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
     5701 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
     5761 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
     5821 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
     5881 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
     5941 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
     6001 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
     6061 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
     6121 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
     6181 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
     6241 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
     6301 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
     6361 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
     6421 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
     6481 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
     6541 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
     6601 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
     6661 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
     6721 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
     6781 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
     6841 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
     6901 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
     6961 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
     7021 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
     7081 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
     7141 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
     7201 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
     7261 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
     7321 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
     7381 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
     7441 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
     7501 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
     7561 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
     7621 agactcagct gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
     7681 aggtggtggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
     7741 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa
     7801 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
     7861 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
     7921 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
     7981 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
     8041 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
     8101 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
     8161 tccatgaaac agacgaagac atcaataaaa aagcaaaagc tatctttgca aatggtatga
     8221 caccaatcct atgctgcggc gaatctttag aaacctatga agcaggcaaa actgctgaat
     8281 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca
     8341 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
     8401 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
     8461 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
     8521 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
     8581 catttttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
     8641 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
     8701 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
     8761 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
     8821 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
     8881 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
     8941 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
     9001 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
     9061 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
     9121 cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
     9181 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
     9241 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
     9301 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
     9361 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
     9421 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
     9481 gaaaaaggtg tttacgtact agctgactca ggcgaaggcg aaaaaacaac tgacgaaatg
     9541 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agatggatta
     9601 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
     9661 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
     9721 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
     9781 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
     9841 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaatgctgg ccaaatcaaa
     9901 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
     9961 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    10021 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    10081 acttagttaa gtaatcgaac tctctacttg ctgaaatatc agcaggtaga ga
//
LOCUS       DACDNO010000081         9964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDNO010000081 DACDNO010000000
VERSION     DACDNO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.081"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..81
                     /locus_tag="IWI22_002428"
     tRNA            8..81
                     /locus_tag="IWI22_002428"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="IWI22_002429"
     tRNA            101..173
                     /locus_tag="IWI22_002429"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="IWI22_002430"
     tRNA            201..274
                     /locus_tag="IWI22_002430"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="IWI22_002431"
     tRNA            288..361
                     /locus_tag="IWI22_002431"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="IWI22_002432"
     tRNA            385..474
                     /locus_tag="IWI22_002432"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="IWI22_002433"
     tRNA            487..560
                     /locus_tag="IWI22_002433"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="IWI22_002434"
     tRNA            579..654
                     /locus_tag="IWI22_002434"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="IWI22_002435"
     tRNA            656..726
                     /locus_tag="IWI22_002435"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="IWI22_002436"
     tRNA            740..813
                     /locus_tag="IWI22_002436"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="IWI22_002437"
     tRNA            838..925
                     /locus_tag="IWI22_002437"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="IWI22_002438"
     tRNA            947..1018
                     /locus_tag="IWI22_002438"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="IWI22_002439"
     tRNA            1041..1130
                     /locus_tag="IWI22_002439"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="IWI22_002440"
     tRNA            1143..1216
                     /locus_tag="IWI22_002440"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="IWI22_002441"
     tRNA            1235..1307
                     /locus_tag="IWI22_002441"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="IWI22_002442"
     tRNA            1311..1383
                     /locus_tag="IWI22_002442"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="IWI22_002443"
     tRNA            1388..1458
                     /locus_tag="IWI22_002443"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="IWI22_002444"
     tRNA            1471..1544
                     /locus_tag="IWI22_002444"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="IWI22_002445"
     tRNA            1556..1627
                     /locus_tag="IWI22_002445"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     gene            1893..3086
                     /locus_tag="IWI22_002446"
     CDS             1893..3086
                     /locus_tag="IWI22_002446"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9169779.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="IWI22_002447"
     CDS             3216..4688
                     /locus_tag="IWI22_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9169780.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="IWI22_002448"
     CDS             4771..5559
                     /locus_tag="IWI22_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9169781.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="IWI22_002449"
     CDS             complement(5626..6294)
                     /locus_tag="IWI22_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9169782.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="IWI22_002450"
     CDS             6409..8121
                     /locus_tag="IWI22_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169783.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9896
                     /locus_tag="IWI22_002451"
     CDS             8124..9896
                     /locus_tag="IWI22_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169784.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2900 a         1781 c         2278 g         3005 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     9901 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     9961 ggca
//
LOCUS       DACDNO010000082         9810 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDNO010000082 DACDNO010000000
VERSION     DACDNO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.082"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(33..2153)
                     /locus_tag="IWI22_002452"
     CDS             complement(33..2153)
                     /locus_tag="IWI22_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9169785.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2383..3561
                     /locus_tag="IWI22_002453"
     CDS             2383..3561
                     /locus_tag="IWI22_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9169786.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3577..4335
                     /gene="aroD"
                     /locus_tag="IWI22_002454"
     CDS             3577..4335
                     /gene="aroD"
                     /locus_tag="IWI22_002454"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9169787.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4358..4843
                     /gene="ybaK"
                     /locus_tag="IWI22_002455"
     CDS             4358..4843
                     /gene="ybaK"
                     /locus_tag="IWI22_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9169788.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4914..6167)
                     /locus_tag="IWI22_002456"
     CDS             complement(4914..6167)
                     /locus_tag="IWI22_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9169789.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6284..7633
                     /gene="gdhA"
                     /locus_tag="IWI22_002457"
     CDS             6284..7633
                     /gene="gdhA"
                     /locus_tag="IWI22_002457"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9169790.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7745..9091
                     /locus_tag="IWI22_002458"
     CDS             7745..9091
                     /locus_tag="IWI22_002458"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9169791.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9399..9785)
                     /locus_tag="IWI22_002459"
     CDS             complement(9399..9785)
                     /locus_tag="IWI22_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9169792.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3127 a         1782 c         2010 g         2891 t
ORIGIN      
        1 attttgtcgc tgctcctttt aaatttattt tatcattcgg aagaacttgt ggtcgttccg
       61 tgaagttctt ggtacgtttg cgttttataa agatcaacag ttgattgtcc gttgtgttca
      121 ctaaagacac tagttgactt gtcaccaaga gatttttcag cttcttttaa tttctctaag
      181 ccattcttgt agtcataatc actggtatca attgggtcta atccactatt cgtatagaac
      241 cgaagaagat caccattatt gatttggtca gaaacagcta actgtttatc aactttttct
      301 ttcagtgaat caatttcatt ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt
      361 tcgttactat agatcgtacc accgtagtaa gtatattttg gtgtaacaaa atcaccatca
      421 cggaaagaaa caagctgttc atgatctttc gagaatagat cttgtcctaa ctgaatataa
      481 ttttttgtat ctacacctag taagtgaagt aatgtaggca aggcatcaac ttgtccgccg
      541 taagtgtgat tgattccgcc ctgtgtttgt ccagggatat ggatcatata agggacacgc
      601 tgcatttgtg cattgtcgta gtttgtccaa gtagaacttg tttttccaac taattcagct
      661 agatttttat ttcttgaatt cgagacaccg taatggtcac cgtaaaggac gatgactgaa
      721 ttatcataca agccgctttc tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa
      781 taattggctg tcgcaaagta accgttgatc gtttcatcat ttgtattggc taacgggaat
      841 cctgcttcgt cattggtgaa ttttgaataa ggataatggt tagatacagc aatgaatttt
      901 gaataaaatg gttgttgcag acgttccaag tattgtacgg attgttggaa aaatggttta
      961 tcatgcaacc cgtattggaa cgaattgtca tcattaacat cataataaga agaatcaaag
     1021 aaataatcat atcctaaatt cttgtatgtc tcgtttcgat tccagaatgt accagcgttc
     1081 ccatggaata ccgcactaat gtaaccttgt gtttgactta aaatatcagg tgcagcttgg
     1141 aaagtatttt ttccgcctaa ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa
     1201 ttttccatca gcgtttccgc atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg
     1261 tcaaaactga acgtgctgtt gctgtggtaa agactattga tgaaaggtgt aacttcatgc
     1321 tggagaccat tctcatctgt caatttataa tcgatcagga attgctggaa gctttctaaa
     1381 tggatataaa tcacattttt tcctctagcg agcccgaaca aatcgtcatt tggtgctgca
     1441 taatgttctt ttacataatt ttctacttct ttcatatcat tgggacttgc ttctgcccgc
     1501 acttggtttg tctggtaagt ttgaacaccg tcataaacca tgaaagcatt caagccaaga
     1561 tatttgacca aataatcacg agagaatgtt cgagtcagca attctggacg atcagcttcc
     1621 gccataaaaa gatttcctga gaaaatcata actgctaatg tagagatcgc aaaagccatt
     1681 cttgctcgga tcgggcgagg gtccaacttg attttttttg caatcagcag aacagtcaag
     1741 ataataaaat cgatccaata gatcacatca tatgggcgaa acagtcgaat ggcactttct
     1801 cccagaccac tcgctacttt cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg
     1861 aattctcgat aataagtgac attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc
     1921 atcatcgtga tataagcaac tctagggcgt ctgatataaa gagcaattcc aagtaataac
     1981 aatgtagttg caatagggtt gataaacaag ataaagtatt gaatgccact ttcgattccg
     2041 agatggaaat caactaaata ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa
     2101 gaaaaaaatc cgagcctctt attcaaaaag gcaggtgttt ttatacattt caagataatc
     2161 ttcctttctg cacttcttaa tcacagaagt gtcctgaaac catgttacac agctcactta
     2221 ttctcattta ttttactagt atgacaatat agtgtcaatt gaaaccagaa gtttatgctg
     2281 tttcattaag gttttcttta agcttcatga aaaaatgagc agtaagacaa gtcctatgtt
     2341 ttttgttata cttgggaaga gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa
     2401 aaaagagcaa gcaatcgatt gaaaaaagga tatccactga tccaaaaaga agaccttgct
     2461 catccccaaa aagaaaccac gcaatgggtg gaattagttg attcgcaaaa ccaatttatc
     2521 gcaaaaggat atctaggaaa gcagaataag ggaatcggtt gggtattgag ccagcagaac
     2581 atcccatttg atcaatcttt ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact
     2641 ttcttttttt cagatgattc tactaccgct tttcgtatat tcaatggaga aggagacggg
     2701 atcggcggtc tgattgttga ttattatgca ggttatgctg ttttttcttg gtataacgaa
     2761 acgttatatg cacatcgtga attattgatt caagcttttc gtgaaatcta cccggaaatc
     2821 ataggaggct atgagaaaat tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt
     2881 tacggaaaag aggcaccgga acccttgatc gtaaaagaaa acggagtaaa atttgccgtt
     2941 tatttaaatg aaggattaat gactgggatc tttttagacc aaaaagaagt acggggatca
     3001 ttagcagaag gtttcgcttc tgggaaaaca gttttgaaca tgtttagtta taccggcgcc
     3061 ttttctgttg ctgccgcaat gggaggggca atcgaaacaa caagtgtcga tctagcaaaa
     3121 cgcagtttaa aaaaaacaca ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa
     3181 aaaatcattg ttatggacgt attcgagtat ttcaaatacg ccaaaagaaa acaattttta
     3241 tatgacatga ttgtattgga tccaccaagt tttgccagaa ataagaaaaa agtctttcgc
     3301 gtagcaaaag attatgggga actggtcaag gatagtctgg atattttagc agaagacggg
     3361 atacttattg cgtctacaaa tgctgcgaat gtctcgatag aacaattcca aacaatgatt
     3421 gaagaggaat tccatgccaa aaatgtttct tttgaacaac ttgctttata tcgtctgcca
     3481 aaagatttcc agacgacgga taccttttta gaaggcaatt atctgaaagt gtttgtttat
     3541 caaattaaaa ataaccatta gaaggagcga tgcagaatgg ttcgggtaaa gggattgaat
     3601 ataggaagcg gtagaccaaa gatttgtgcg cctcttatcg gggccgtccg agaggaagta
     3661 ttacgtgaag cagttcttgc acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat
     3721 cattatcgtg aagttttttc ttttgatgaa gtagcagaaa cattggttct tatccatgag
     3781 atgcttgatg gactgccgtt attatttacg tttcgtacct tagctgaagg cggagaacaa
     3841 gacatatcga tccgtcagta tcgcgacctt tatgaacatg tgatacacac aggattagta
     3901 gatatggtcg atatcgagtt gttcaaagta gaaagtctag gaaaaagact gatagaggaa
     3961 atcaagtctt taaacattcc tttaattatc agtagtcatg attttaaaga aacaccagca
     4021 gaccctgtct tgctttaccg attgaatgtt atggaacatt ttggagccga tatcggaaaa
     4081 ctggcagtaa ctcccaataa tgaacgagat gttttgcggt tgatggagtt gactagacgg
     4141 gcaaatgctt ttgtctccat gcctattatt acgatgtcca tgggaaatct tggtaaaatt
     4201 tctcgtctgg ttggcggaac gacgggttcc gttatgacat ttggttcctt aagcagcgat
     4261 tctacttctg ctccaggaca attaccggtc aaagcattga agcaaattat tcaattatta
     4321 aatgaaccgt tgtagaacaa gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa
     4381 aacgaatgca atccgtatgg tcgaacaaaa aaagattccc tatacagagc acgaatatga
     4441 gtgggacgaa agccacctaa gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc
     4501 tcgtatattt aaaacactgg ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat
     4561 accgggagaa gccgaattga atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt
     4621 agaaatgctc catttaaaag atttggaagc aacgaccgga tacatacgtg gtggctgctc
     4681 gcctataggt atgaaaaaac tatttcctac ttatttagat caaattgcag aaagctatga
     4741 acaaatcatt gtctcggctg gcagaagagg gctacagatg gagttagcac ctcaagatat
     4801 ctgtgcatta acgagcggac aatttgcaga tataaaacaa taaatcatac gtataaaaga
     4861 aagaaggagg ccgggatgtt gtttgtccag tctccttctt tcttttttat acattatgct
     4921 ctttgcccgg cattcattcc tgctacatgc cctgtagcga aagccgctgt gatattaaag
     4981 ccgccagtat aaccattgac atccagtaat tctcccgcaa aatacagacc atcaatgatt
     5041 ttactctcca atgttttagg ataaatttcc tttagagaaa cccctccgcc agttacaaat
     5101 gatttctcaa taggaaaagt tttgttgatt gatagcttaa actctttgca tagttgaaca
     5161 aaatcttgaa tttgtttttg agaggcttgt tgtcctgtca gatgatccat ttcaagccgc
     5221 tcaagaaaga aaactaacaa tcgctcaggc aatataccat gccaagcatt gaccaagttc
     5281 tttttggttg ctttactttt ttctgtcaat atatgaataa gctcttgttt ggtttgtgta
     5341 gggaaacaat ccagagaaag tgtcactggt tctcccgttt tctctaattc tttattgaca
     5401 aaacttgaac aacgcagtgc agccggacca gaaataccga aatgggtgaa aagtaaatcc
     5461 atcgtatgtt cagtcaatac acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt
     5521 aaagaaagcc cttgaagtgt tttctcctga atatacggtt catccgatat caagggggac
     5581 tctgtcgcat aaagaggcgt caccgtgtga cccactcgct tcacgatctt gtacccatct
     5641 ccagtagaac cagtagatgg ataagtccgt ccaccagttg ttaagataat ggcaggagca
     5701 tggaactctt cgaattccgt gcgtataccg taaattttat gttctttgtg gatcaatttc
     5761 tctactcgag tagagaatag aagcgtcaca tctagttctt tcagacgatt gagcaatgct
     5821 tcgataatcg tttttgattt atttgttaca ggaaacatcc gtccatgatc ctcttctttt
     5881 aaatgtacgc cttgtgattc aaagaagttc atgatatcga aattattcca ttgcgcaaac
     5941 gtactatata aaaactttcc atttccagga atatgtgcaa tcaaatcatc aacaggacga
     6001 ttgttcgtaa cattgcaccg accaccacct gtcatcagta gtttttttcc tgcttttttg
     6061 tttttttcga tcaagagtac gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc
     6121 ccgcttgttc cagcgccgac tacgatcaca tcatacagtt tttccattcc ctcacctcaa
     6181 ggaaagtgta gcatatctga agaaagaaaa cagaaaaaaa tgttttgagc caaaagagaa
     6241 aatgagctaa aatgagggaa gataagcaaa ggagcgtgga acgatgacaa acgcaacaga
     6301 atatgttcaa gcaattcaag aaaagctaca tcaaaaggat caagggcaag aagaattttt
     6361 acaagcaatc gatgaattca tgcccacggt tatgtctttt ttggacactc atcccgaata
     6421 tatcgaaaag aatatcttag aacttttaac ggaaccagaa agagttattc aatttcgagt
     6481 gccttggcaa gatgatgaag gaaactggcg agtaaataga gggtaccgta tccaatacaa
     6541 ctcagctatc ggcccttata aaggtggatt gcgcttccat cctagtgtca atctaagtat
     6601 cttgaaattt ttggcttttg aacagatttt caagaatagt ttaaccggtc tgccaatcgg
     6661 tggaggaaaa ggcggaagtg acttcgatcc taaaggaaaa tctgataatg agatcatgcg
     6721 tttttgtcaa agtttcatgc tggaattgtc aaaacatatc ggcccatcga ttgatgttcc
     6781 tgcgggagat atcggtgtag gagctagaga aatcggctat ttgttcggcg aatataaacg
     6841 attgaaaaaa tatgatactg gtgtgttgac aggtaaacct ttggattttt ggggaagtaa
     6901 gatacgtacg gaagctactg gttatggatt agtgtattat gtcaaacatc tattgaacga
     6961 agaaaaagat tcatttaatc aaaaaacagt ttttgtttcc ggaagcggga atgtagccat
     7021 ttatgcaatc gaaaaagtgc aagagctagg cgggaaagtt gttacatgtt cagattctag
     7081 cgggtacatc tatgatccag aaggaataga tgtgaaacta ttgaaagaag tcaaagaaat
     7141 caatagacaa cgtttgacgg aatacgcaaa ccaacgtcct tctgctattt atcatgctgg
     7201 agaatctgtt tggacattga aagaaaaagc ggatatcgct ttgccatgtg ctactcaaaa
     7261 tgaaatagac gaaagcttgg ccgaaatctt agtagaaaat gggatcaaaa tcgtagcaga
     7321 aggcgctaat atgccatgta cagtaaaagc tgtttctgtt cttcatgaag caggcgtgtg
     7381 gtattgtcca ggaaaagctg ctaatgctgg aggagtagca gtatctgctt tagaaatgag
     7441 tcagaacgtc cagcgattga cttgggaaag agagcaagtg gatgagcagc tggatcaaat
     7501 catggaaacg atttaccata cgtgtcgcca aacagcagcc gagtacggcg acgaaaaaaa
     7561 tctgcttctt ggagcaaatg ttgctggatt tgaaaaagtt gccaaagcaa tggcagcaga
     7621 aggtttagtt tgaaaaatga aactttcttg gaaatattgt cagcattttg tttatttgct
     7681 tgaaaaataa taaaaaaaaa cgtaaagtat tacctgtaga gtaaacctat aaggaggaga
     7741 acaaatggca cacattcatt ttgactattc aaaagtagca ccgtttgtca acgaacaaga
     7801 gttagggtac atgcaaagcc aagtaacagc agcccacaac gaattacggg aaggtactgg
     7861 cgcaggaagc gacttccgcg gctggatcga tttaccaacg aactatgaca aagaagaatt
     7921 tgcacgtatc aaagaagcag ctaaaaaaat ccaatctgat tctgaagtat tagttgttat
     7981 cggtatcggt ggttcttatt taggcgctcg tgcagcgatc gacttcttga atcacacatt
     8041 cttcaattta ttagatagca acacaagaaa agcaccacaa atcttctttg ccggaaattc
     8101 aatcagctca acatatattg ctgacttgat cgaagtaatc ggtgatcgcg acttttcagt
     8161 aaacgttatt tcaaaatctg gaacaactac tgaaccagcg attgcgttcc gtgtattcaa
     8221 agagcttctt gttaagaaat atggacaaga agaagcaaac aaacgtatct acgctacaac
     8281 agataaagca aaaggtgcag ttaaagtaga agcagatgca gaaggttggg aaactttcgt
     8341 gattcctgat gacatcggcg gacgtttctc tgtattaact gctgttggtt tgttaccaat
     8401 cgctgttagt ggtgcagata tcgatgcatt gatgcaaggt gccgctgatg caagcaaagc
     8461 atactcaagc gacaaactag aagaaaacga agcttaccaa tacgcagcaa tgcgcaacat
     8521 tctttaccgt aaaggcaaag taactgagtt gttgatcaac tatgaaccag gaatgcaata
     8581 cttctcagaa tggtggaaac aattgtatgg tgaatctgaa ggaaaagacc aaaaaggcat
     8641 ctatccttca agcgcaaact tctcaactga tttgcactct ttaggacaat tcatccaaga
     8701 aggacgtcgc aatatttttg aaacagttgt gaaggttgaa aaacctcgta aatcaatcac
     8761 aattcctgaa caagcagaag atttagacgg tctaggctac ttgcaaggaa aagaagtgga
     8821 cttcgtaaat acaaaagcat ttgaaggaac attgcttgct catacagacg gtgacgtgcc
     8881 taacttgcta gtcaaaattc cagaaatgga tgcttataca ttgggttata caatgtactt
     8941 ctttgaaatt gctgtaggta tttcaggtta cttgaatgga atcaacccat tcgaccaacc
     9001 aggtgtagaa gcttacaaga aaaacatgtt cgcacttcta ggcaaaccag gctttgaaga
     9061 tcttgctaaa gaattgaacg aacgcctgta agataagggt ttttcgctag atttaaataa
     9121 tcagtgatag aaacatgttc gctttaaaat gaaaactggt acccatttag gtactcaaac
     9181 atactgcatc gtttattcag aataaggggc tgtgacaaga cgtttgtcac agtcccttgt
     9241 ttgtatagaa atcgattgat tgccttgctc tttagtttgt tttaggactt taagaacagt
     9301 gaggctgact tcgaaggtta attctgtttt tttgttttct agaatcagta agcaaaaaag
     9361 cactcacttc gagtgctttt ttgcgatata ttcttagctt aagataggcc taatttttct
     9421 tttacagatg agggtacctt atcgctggta attacttttt tctctatgat ccgcttttcg
     9481 cttgctttca actgcaagat cgttccttcg gggtaagcag aaccattggc agagtcacca
     9541 gaagtaatga catcgaaatc taactgtctt ttttcaccgt tttcagcata agcatctacg
     9601 ttgtatttaa aacctcttcc aattacttcc ccattatctg ctttgatatc agtttctgca
     9661 ggcattggag ctttgactac cgcataataa tctttaccta cataagtaga ctgatagtag
     9721 tcccaacctt tccatcctat aaaagcaatc aatatgataa cgatcaaccc taaaattttt
     9781 ttcatgaaag atctcctttt ttatttttat
//
LOCUS       DACDNO010000083         9702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDNO010000083 DACDNO010000000
VERSION     DACDNO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.083"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1023
                     /locus_tag="IWI22_002460"
     CDS             <1..1023
                     /locus_tag="IWI22_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9169793.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="IWI22_002461"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="IWI22_002461"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9169794.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3389..3985
                     /locus_tag="IWI22_002462"
     CDS             3389..3985
                     /locus_tag="IWI22_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9169795.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="IWI22_002463"
     CDS             3998..4897
                     /locus_tag="IWI22_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9169796.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEDIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="IWI22_002464"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="IWI22_002464"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9169797.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="IWI22_002465"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="IWI22_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9169798.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="IWI22_002466"
     CDS             5430..5663
                     /locus_tag="IWI22_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169799.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="IWI22_002467"
     CDS             complement(5822..5944)
                     /locus_tag="IWI22_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9169800.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6134..6358
                     /locus_tag="IWI22_002468"
     CDS             6134..6358
                     /locus_tag="IWI22_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169801.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            6801..7007
                     /locus_tag="IWI22_002469"
     CDS             6801..7007
                     /locus_tag="IWI22_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9169802.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7007..7258
                     /locus_tag="IWI22_002470"
     CDS             7007..7258
                     /locus_tag="IWI22_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169803.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            7439..7711
                     /locus_tag="IWI22_002471"
     CDS             7439..7711
                     /locus_tag="IWI22_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169804.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            8156..8335
                     /locus_tag="IWI22_002472"
     CDS             8156..8335
                     /locus_tag="IWI22_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169805.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            8394..8750
                     /locus_tag="IWI22_002473"
     CDS             8394..8750
                     /locus_tag="IWI22_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169806.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         3490 a         1613 c         1994 g         2605 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     2341 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatcct attatagctt atgatccaga aagaacttta tgtgacattt ggaatccctg
     3841 gtataccgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactatatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaatttta ccaacagaca aaacgatgcg
     3961 ttcttatatt atggctttaa attaaaggag aagatacatg acacctacgc agttaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttgattg gctcaaagta tggcattgaa aatcgcacaa ccaaagatat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttaacaacgg ttctaaacga
     4261 aattttttca acgccgacga aagaagacat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagagaagcc gattactatc caggatttag cttacgtgtg ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgtgactac aggtgactcg atctatcctg caacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttgtcaga ccggcaacaa gttgcctttt atcaccaaaa
     6661 agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtcttgtcgc ttttcgttga
     6721 ttcattctgg ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct
     6781 aagaggaggc agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg
     6841 aattattccc aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga
     6901 aatgtatcaa aagctattgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt
     6961 cttatggcga ttatatttta attgatgaga aaggaaagaa acgctgatga aaacacaaca
     7021 caaattattg actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga
     7081 aaaagtagca gaagttttac ttattccgcg gtactggacg atagaaaccc ctttagtaga
     7141 ggatgcaccg actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta
     7201 tcaagaaaac ttggaaactt atgaaaacga atattggtta accaaattag agaactaaaa
     7261 aggagaaaaa aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa
     7321 aatggcacaa aaaatggagg atatgacgtt cctatacaac gaaacacgtg ttccaaaaaa
     7381 acattataaa gaaaaattgt cggtagctgt ggaagaaatg attgaataag gtgtcgaaat
     7441 gaatcttatt gcgacgtact atcgaacact ggaagaactg aaaaagcaaa atgcaaaatg
     7501 gtttttccaa gccttactat gcttggaagt aggtgtcaaa ccaagcacta ttaaaccttc
     7561 tgaataccaa gctttagaac taacgtatgc gaaatttatc gaaacgaaaa aagccaagac
     7621 tgtttcaagt gaatggttag attattttga aaatatcaat aagtatggcg catattacac
     7681 catgaaaaag gaagacaacg aaaatgaata aggtgattta tcaactaaca acggattcca
     7741 tatcaacgat ttgaaagaaa aaaaggtcaa taatcattta taccgaaaag tgagaatgtg
     7801 tgaaacaagg acaaaaaaag cctagtaact aaggaaatgg ttcttatttc gcaccaatcg
     7861 ctccaaccgc ccccgccgct taggtggtgc gaaataaacg ggcaaagccc gataacttaa
     7921 gagaaagaag gtgatccatt tgaacaagat tattccctta ttgttagtac tgtttgttgg
     7981 cgaaatactt atgcgagata gtgcagctgt acagttaaac ctcgctgtat ttttggtaat
     8041 actggtgttt tgtttgatct tttggcggtt tgtattagtc ctcgtgagtc tactattcaa
     8101 gatgattgca gccatatttt tcatagggtt atttcgatga aaggagtgag taccaatgcc
     8161 aattgcaata ggaaataaac ggttacccgt gacattagat gaaaagagac aaaaagaatt
     8221 gcagcaacta aagcagaagt atggcaaaag tgaatctaag attatgtgta ttgcgttaga
     8281 tctattgatc gcgcaagaaa aagcaggatt tgatgtacca gcgctaaaaa agtgacgtca
     8341 ctttttaatt tgatctatat aagtgatatc acttttacca aggagggaat taaatgttaa
     8401 aaactaaaaa gaattcgtac aaattaaagc ttagtatact atctattatt tgtcttttag
     8461 gcatgataat tagtggtcat ttgtatagag tggcatatac gcagtttatt gagtacaagg
     8521 ttcactcttt aggattagct ggggatcgat acgttgaata tgtccattat tcattaaaat
     8581 gttcttcata tattcttctt tggcttattt tttcattgtt tgaattgcta aatttaatag
     8641 caatatcttc atatcactta gataagctaa ataccaaatc tagccgttat aacgaagtgt
     8701 ttaaggaaaa tgtgaagtta gaaagtgact taaacaagct aaaaaaataa aaagaagaaa
     8761 acataagtta actaaaatta ttttattaag aatgatggaa tataaggata aggttaccaa
     8821 cataacgatc attacttctc tgatatgcta tggacttcta tcaatcagta ttttgtctat
     8881 agattatgga gtaactatac tgcaagcctt agttttggct atgcctatgt atctatcttt
     8941 gtttatcatg catgaaatat ataagctatt tgttttatca aaaaatgtag aaatacgggt
     9001 aaatattttg gtgaatagtt atcaaaaaga actcaaagaa agaagacggt ggcggtattt
     9061 attgaaagga tgaaaaagta ggatttgaca tactggtatt cagaaagtgc cgtcactttt
     9121 aaaacaatgg aaaaggtgga gataagatga agaaaaaaca aactaaacta tcattagcag
     9181 atatttaaca cagttaaccg caactgaaga tggtgttgtt gaatttgaac aatctgaaat
     9241 tgcggtagta gatgatcgtt accttaaaat gccttacttc tttgaccaag caaaggtcat
     9301 ttgcctttgt cactacgatg gggtacgtga ttttaggcgt taaaaagtag tgattatatc
     9361 aactttacga gtaaatataa atttagacat gaaactgaat tatccaatgg aaaatcatgg
     9421 tttataatct agaaaaatta tagtaatgat tggattatat atattctttc gtattttcgt
     9481 acaactgtag cttgtcaaaa ttgagaaatt taaccgatta ggtactttac atcgttattt
     9541 gggtaatttc taaaacatga agcttaccaa cgaataaact gagactatct tattaattga
     9601 tcaatataag aaaatatctt tgaggagatg atgtactatg ttttcgtcta tccacgattg
     9661 gttaggtggt cttttcggcg gtggaggtaa aggtttttaa ta
//
LOCUS       DACDNO010000084         9570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDNO010000084 DACDNO010000000
VERSION     DACDNO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.084"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            202..396
                     /locus_tag="IWI22_002474"
     CDS             202..396
                     /locus_tag="IWI22_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169807.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            665..964
                     /locus_tag="IWI22_002475"
     CDS             665..964
                     /locus_tag="IWI22_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169808.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1061..1495)
                     /locus_tag="IWI22_002476"
     CDS             complement(1061..1495)
                     /locus_tag="IWI22_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9169809.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1727..2437)
                     /locus_tag="IWI22_002477"
     CDS             complement(1727..2437)
                     /locus_tag="IWI22_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9169810.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2427..3287)
                     /locus_tag="IWI22_002478"
     CDS             complement(2427..3287)
                     /locus_tag="IWI22_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9169811.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3292..3930)
                     /locus_tag="IWI22_002479"
     CDS             complement(3292..3930)
                     /locus_tag="IWI22_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9169812.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3945..4244)
                     /locus_tag="IWI22_002480"
     CDS             complement(3945..4244)
                     /locus_tag="IWI22_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9169813.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4287..5771)
                     /locus_tag="IWI22_002481"
     CDS             complement(4287..5771)
                     /locus_tag="IWI22_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9169814.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5791..6252)
                     /locus_tag="IWI22_002482"
     CDS             complement(5791..6252)
                     /locus_tag="IWI22_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9169815.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6270..7337)
                     /gene="ulaG"
                     /locus_tag="IWI22_002483"
     CDS             complement(6270..7337)
                     /gene="ulaG"
                     /locus_tag="IWI22_002483"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9169816.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7555..8325
                     /locus_tag="IWI22_002484"
     CDS             7555..8325
                     /locus_tag="IWI22_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9169817.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8598..9425)
                     /locus_tag="IWI22_002485"
     CDS             complement(8598..9425)
                     /locus_tag="IWI22_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9169818.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2987 a         1953 c         1493 g         3137 t
ORIGIN      
        1 ttatttgtct cgcttaatta tacaaacggc gttttaagaa caatagcctt ttattatata
       61 tattattcaa attagttata tcgagtgaaa aaaactttca aattgattct ttttatttat
      121 cataaagata tagatacata atcattttca cttacatata tttgaatatt aactaaaaac
      181 agcaagaaag aagaggatca aatgtattat gtagaagtaa aaacaaaagg cgtcaaaaac
      241 aaacaatatg tgaaaggtat cagtaacgaa tatcctttac ttggttcatg gaaagaagct
      301 gctccttttt ccaaaccatg tgccataaaa ataaagaacg agctagaaaa agaactgact
      361 tgtggtaaag ctgttgtaga tatcatcgaa aagtaaatcc aatgtataga aaggaagatc
      421 acacatgaaa agcgacgtac aaattgctaa gattatagaa ttagaagaag aagacgcagt
      481 gttgacaatt gaaaaaaaat aaatgtttgt ataccatatg attatttcaa tatgtatcct
      541 ggaattggag atctcgtaga gctatatcaa gatgaatcaa atatacttgt cattcatcat
      601 gaaatataga gacttataaa attgtttatt tttataatgt atcagtatag aaaggaaata
      661 aaacatggat tatactgttg aatttgacag tgaactactt aagcaatttc cacaagaaga
      721 acatttcatg tataaagaag caatttccca caaagtgaca gagtatttgg agcataattt
      781 ttttcgtata aagccagtgc gtactcgttc ggcaaaagga atatacgaga tgaaagtcaa
      841 aattaataaa aaaaattatc gcatagcatt tgctttaaaa aaaaatcatg tgttcatttt
      901 ttatattagt tcgaatttgc aaaaaattgt atttgataaa gaggtgagta aaaaaataaa
      961 atagctgagc attggaggat agattgagtg atactaagtc tatcctcttg tttttctaaa
     1021 gaactaagct agaatttttt cttttgtacg ttattatatg ttatttgtca ttaccaaaaa
     1081 gcaccttttt tcttctagta tctatgacat aagaaatacc acccatttcc tttacttgaa
     1141 caaatcctac tgattcataa aatttttttg ttttctcagt atcatctgtc aataattcga
     1201 tttggactgg tccataacta gtgatcttct ccaaagcttc attcaaaagc actttgccta
     1261 ttccttggcg ctgatacctc ggatgaatga ggatatcttg aataaatgcg atcgtcgcaa
     1321 aatcagtgat ccaacgaacg attccgataa tcttcccttc cccattacgc tttatcagag
     1381 attcagattg ttcaaatgct ctagctaacc ttgcagtgtc ttttgtgtaa ttcgtccaac
     1441 caacgctctt atataattca gaaatctcat cgatcataag ggaatcaata ttcatttttt
     1501 tcttcctttc ttatacaatt ctccctctaa taatacagtt ctttgactat ttaatctatc
     1561 acatataact ctcctgtaaa gattctctcg attccttaaa attcctcttt ccttttagaa
     1621 tgcatacttt ttgataaata aaaaggttgt gataaatttt cttcacaacc tctgtttctc
     1681 tctatcaagt gttatctttt aattttctac ccttacccta gcatctctat tgcccgtagt
     1741 atgcatactt tccatgtttg cgaaaataat gtttatctag taaatattgc gggatttcac
     1801 atatattggg gttgatcagc tcgttttctt tggccatcag acaaatttca tctaagatca
     1861 aactgttttc tactgctttc ataggtgaat ccccccatgt gaacggtcca tgtccataga
     1921 caagtactcc agggacttcg tttggatcta attttctttc cttaaacgtc tcgacaatca
     1981 cattccctgt atgaacttcg taagcttctc ttacttcttc tttcgttaat tggcgtgtac
     2041 aaggaacctt accataaaaa gcatcagcat gagtcgtgcc ataagctggc aaatcgcgtc
     2101 ctgcttgcgc ccacatgacg gagtgcgtgg aatgagtatg agtaatagca gttatatctt
     2161 caaaagtttg atacaaaaca acatgtgtgg ggagatctga agatggtttt aagctatctt
     2221 cttctattat atttcccgac aaatctgtta ccaccatttg atctgcttgc atgcattcat
     2281 atctgacacc gcttggctta atgacgatga ctcctagctc acggtccact tcactcacat
     2341 tgccccaagt caatttgact aatccgtatc gcggcaatgc cagatttgct tcaaggacac
     2401 gttgcttcat tgctgcaatt ttttcttcac aagacatcat agccagcctc cttcaattta
     2461 gaacttaagt agtcttttgc ctgctggatt tctcttcgaa agtcttggct tttttcactc
     2521 cacatttcaa tcagaaatgt tccatcataa tctagacgtt tcaaatgtct taataatcca
     2581 agaaaatcaa cacaaccgct accaaaagga acatcacgaa actggcctga atattctttt
     2641 gttacagggt aagtatcttt taagtggata gcagtgatat aatcgatccc tttctcaagt
     2701 tctcgagccg gattattttc cggccatgcg gacaaattgc ctaggtctgg atatacttgt
     2761 agaaaaggtg aatgaatctg ttctttgatt tctaaaaatt ttgaaatgga gttcataaat
     2821 ggatcatcca tgatttccac ggaaagaatg atgccatatt tagaagcttc ctttacgctt
     2881 tcttttattc cttcgataaa gtcttctctg gaattcattg atttctcttc ataataaaca
     2941 tcgtatccag ctatttggat tactttgacg cttagattat gcgccaaatg gattgctttt
     3001 ttcattagtt gtttcgctgc ttttttcttt tctggatcag cagaaccaaa cggaaatcgc
     3061 cgatgcgcac ttaaacaaat cgaatggata cgtacaccgg agctgaacat ctcttctctc
     3121 agctcagcta tcttttcttc agaccaatct aatcgtgcta accgctcatc cgtctcatca
     3181 atcgacatct cgataaaatc gaagtccatt tcttttacaa gggaaaaacg ttctttccat
     3241 gaaatatctt tgggtaacgc tttttcataa atcccaattt gagccatttc cttaccccca
     3301 gatacgaatg atctcatctt tgaagttttg tgcggccttc atcggatcat ttgctgctgt
     3361 aataccacga ccagtgataa aggtatacac atcgatccct tcaaataagt gtaatgtgtg
     3421 tggatttaat ccaccagtta cagaaacatt aaagcctagt tcaatcagtt ttttgatctt
     3481 cgataaatct ttttcacccc aagtttcacc agataacagc gcatcacggc tttgatggta
     3541 gattacctgc cggataccga tgttatgcca atccatggct tgttcaaaag tccaattgcc
     3601 gtagagttct acttgcaatt cacctatttc tttttgtgct gcctccatag ttggaatcgt
     3661 tgcacaacaa atgactgtca tccagtctgc tcctgctttt gcaacattac tcgccactgt
     3721 tcctccagcg tctgcacatt tcgtatcagc gactagtttt ttatctggga acatactgcg
     3781 aatacagcga atcgcttttt gtccttcctg taagcaaaga attgtgccca cttcaataat
     3841 atctactaca tcaccaactt tcataacatc tcctaatgca tgttctaagt cattgtgatc
     3901 taacgcaatt tgcaaattcg gcctgctcat cttagttcct tcctttactt gtttttttgg
     3961 taccactttg tttcagcaaa tttttcactg atttctttga ctgacatcac atttttgata
     4021 ccgataactg taactccttt tttctctgcc tgagtaaaca tattcaaaaa attgactggc
     4081 gtaaaaacta catcgtattg gccagctgaa ctttttccct ctgaaatggc acaatgatgg
     4141 attttggtaa ttggtacgtt tagttctttc attgcttttt ccacacttct catcatcatc
     4201 aagcttgttc ctgatccatt tgcacacgat actaaaattc tcatcttaat ccctccatta
     4261 tgagtgttgt ctaatcagat tttcttttat actctctgct tttctgcata tgtgtcatag
     4321 tcttctacaa tcaaaaaata accatcagga tttttccgat attgtaactg cggaatagcc
     4381 aataaaccaa tgacaactac ggcaattcca gcataaccta ggactttcat gattactgta
     4441 aacaatggcc aagctgttgc ccagtcgaac atcccgatat aaccaccgta tgttgacatc
     4501 ccaatccatg tagcgaataa tgctgaaccg cctacttgta tgattcctga aagaaacggg
     4561 aacaggcaag cggctttgaa tccacctcgg ttattggcat aaactgctat aacggcatta
     4621 tcaaagaaca acggaatgaa accggctatg acgatcgttg gtgatttcaa taaaatcagt
     4681 aaaccaatca tcaaaaactg tcccaaagca ccaaacaaga acccaatcgt taccgcattt
     4741 ggcgaaccaa agccaaaagt agcagctacg tcaattcctg gaacagctcc tggtaaaatc
     4801 gtgtttgaga ttccttggaa agattgagtt agttcatcga cgaatgtccg aacacctaat
     4861 tgcaagatcg ccagatatac cgcaaaattt aaagaagtcg tcataatgta gaataagaaa
     4921 ctctgccctt cttgcatgaa ttgcgcttga atcaaataat ctttgcctag tacaatcaga
     4981 atgatcccaa agaaaaatag catgagtaac gaagtagcaa ccatattctc attgaaaatc
     5041 gacaagaatc ctggtaattt gatatcttca atttttttgt cttgcttgat tgatctattt
     5101 ttcttttcat cccgcttttt catccactca gctaattttg caaatataaa gattccgaac
     5161 atttgctgat gtgccactgc aaatccggca ccttctgtca attcctgcgt gattcctact
     5221 gttaagttag aagctactgc ccaataacaa cctaaaatca gtcccataaa aatcaatact
     5281 tgtatcctac ctagatcagg aaagcaaaac agtaaaatcc aaaaagcggt agctgcttgt
     5341 tgaacctgaa cgtttccggt agtgaaaacg gaacgcagtt tcgtatattt ctgaaaacgg
     5401 actaataaga tatttaaaac aaatgcaatc agtaataaaa tcatcacatc tccaaaggta
     5461 cggccaaatg tctccattaa tccagcatcc acagcatttt gtccaaaata aggatctgta
     5521 accatggcat ccaaattgaa ccgttccttt agtccaacca agattggccg aaaattattg
     5581 accaaaccac cagagccaac agtcaaaata aagtaaccga ccgttgcctt taagaatcca
     5641 gcgatgcatt catgaagcgg gcgtttcagc aaaatatagc ctaataacac gataaaacca
     5701 atcaggtatg ctggctgcgt caagatattt gttgcaaaat acgtccaaat gcttaacaaa
     5761 aattccccca tcttttttct cctctatcaa ttaattatat tctgccatca caacttggta
     5821 atcatcgatg gagttcgtag agatgagacg ctcaaccaat ccatctgtca ttaataattc
     5881 ggataatttt tggatatttt ctagatgttc ttctggatct tttgctgcga gtgtaaaaaa
     5941 caaactagcc gttttctcct cattctcccc ttcactttca aaacggattg gctgtttcaa
     6001 cttggtaaat gaaatagctg ttccaaaaac accatcactt ttttctgaag aatgcggcat
     6061 tgctacttga ggtgcgatca caatataagg accgtatttt tctacacaat ggatgatctc
     6121 ctccacatac gtttcatcaa taatttcctt atcgatcaat acaagacagc tttcggcgat
     6181 agcttcacgc caatccgtga tttctttatc tgggtaatga atcaattgat ggtcaaaaaa
     6241 gtatttcagc aagaaactcc ccttctttct tataaaaacg acggaaacgg tgtatcattt
     6301 tcaatcgaga agacatcttc aaatcctcga taaaaattaa actcaagatc atctttgttt
     6361 gtcggataga tgaatttccc gcctacttgc cagatataag gtttaaatcc atacttcagg
     6421 cgatcctttt tgaatttcca aatttcagtg atctcttttg gatcagccat aaaattagac
     6481 caaatatcgt agtggacagg aatcactact tttgcattca atgactccgc catgcgcagc
     6541 atatcaacag aagtaacttt gtctgttatc cctcgtgggt tttcaccata ggcccctaaa
     6601 cagacatcga ttttgtgttc attaccatgt ttggcaaata aattggagta atgtgaatct
     6661 cctgcatgat aaagattccc tcctgatgtt tcgaataaat agtttactgc aatttgatcc
     6721 atctcctgag gcatttttcc ttttaatacc tcttctggat cttcacaagt gaccaacgct
     6781 gtccgatcaa acgcttctaa agctactaca ttgatgtctt tgattttgac tgcatcacct
     6841 ggctttactg taatcgtttt ttcttcagga attccccatt tttgccacgt gcgaaccact
     6901 tcttttggcc caatgaatgt tgcttcagga caattttgat gaactgctgc ggctgtatgg
     6961 atatctagat gatcggaatg gatatgtgta acgattaaag cgtctacatt ttttatcgca
     7021 aaaggatcga ttacgaaagg ctgagtacgt aaattaggct gcatattttg aacgccactc
     7081 atacgcatca tctgatggcc ttttttcatc atgccattcc catgactacg ttttcccgtc
     7141 ccacaccata gatcacataa aatattcgtt ccttcatgac ttttcagcca aattcccgta
     7201 cagcccagcc accacatagc aaaagttcca ggatccacta cttctgtttc gatttcttca
     7261 ttcaagtatg tgccccactc aggaaatgtt tctactaacc acttttcttt tgttacttca
     7321 ttgacagttt tactcatttg cttgctcccc ccatatcgtt gttccaactt acaagtaaat
     7381 attaatgtgt atcagcatcc atgaaaaccc ttttatatga ccgtttgttt tttatatgat
     7441 tttctttcag taatcaatga ttgtttcagt ataataaaga cagatatgaa tgtttaaatg
     7501 aacaactagt caaataaatc catggatcaa gatgtccctc aggaggcaaa agtcatgaaa
     7561 aactcacttg tcaatattga aaaacgtcaa aaagaaattt tggaactgct tcagcagact
     7621 gaacaactat caactttcga attagctgaa tcccttgatg tatctattag tacgatacga
     7681 agagatttaa atttattaga agaaaaaaac gagatcatac gcaattacgg ttattgttct
     7741 tataattaca aaaaccagac tgattttgat caatctggtc ctgaaaggat taaacaagcg
     7801 attgccagag aagctagtaa atacattaac gattatgata cgctgtttat caattctagc
     7861 tcgactgcgt tgaaaacact ggattttctt caatctaacc atttgacagt cattacaaac
     7921 aacgtgaaaa tcaattactc tccgcacaaa gataattgta gttatatttt aactggtgga
     7981 gagatgcgct ttccaaaaga agcactagtg ggcgacatcg cattaaatac gatttcttca
     8041 gtcaatgcag acatttgtat cattggctgt agcggagtcg atgtagtaca tggggtcaca
     8101 acaaagatat taaatgaatc aacgatcaac gaaatgatgg tcaacaagac aatcaaatgt
     8161 aagattttag tcgctgatca tcgaaaaatc ggactgacat caaagttcaa gattgctgat
     8221 atttcagtct ttgattattt gatcactgat aaattttgtt ctgaaaaagt attagaggag
     8281 atcagaaata cggggataaa aaccattcaa gtagattcat tttaagaacc aggacgtaaa
     8341 tatgtaaata ggattatcaa aaaaatgaat aacaaattta tcgataaatg aaaaacacgc
     8401 tacgctcaaa ttgataaagg aatcccaaga tgtatccatc caatcgatac catcataatc
     8461 tattgcttct tttataaccg ttatatgttg cctaaatagg aatcaaacaa tatcgaaaaa
     8521 aggaggtatg acaaaagtca cacctccttt tttcgaataa acggtattca aatcagaaca
     8581 ctgatttcac aaccacttta tttcaggtcg ttttcaatca cctttaccgc gtaatcattt
     8641 ccgttgaaac gcgctatttt ttttgcgtcc atatatcttt ctaaataatc atcttctcct
     8701 aattgcttat ggcaagaagc aatttcataa agcaagtatt ccaatgcata agtcgattta
     8761 tgtttcttat tatactcaat acccttctca cataattgga ttgctttatc ataatcctcg
     8821 attttacggt aataggtggc gatatcataa tatacaatgc agaccatcag attatcaaaa
     8881 tcagtctcac tcaatatttt aagtgtacgc tccacataaa ctttcgcaaa atctaattct
     8941 ccttttgaca agtagatcat tgccacgttt gcagctgctt gtaagtgatg gatatctgtt
     9001 tcggtcgttt gttccaacac ttgattaaaa taaaataatg ctgcatctgg ttttttcgca
     9061 taaagatgat caagcagtcc ttgataaaat aaaatttgat ttaaaagatc agaattactt
     9121 actttttcct gatcgatcgt ttgtaaaatt ttataagatt cttggtattt tgatacagaa
     9181 ttcaatgatt gaatctcatt tatttttttc ctgattttgt cttcttctgt ttctactaat
     9241 atatcggaga tctcgacatt cagctttttg caaagcgcac ttaagatatt gatgcctggc
     9301 gacatattct tgttttcgat attgctgaca gttgcttgtt cacaaatgcc ttctgctaat
     9361 tgtacttgtg ttaacccctt tttctttcgg tattctttta gtaatgctcc atctacttta
     9421 atcaaaacaa tttccttccc tttaaaaaat tatttctcct ttatatcata cacaaaaaaa
     9481 agttctaaac aaccaaaaat gtttagaaca gaataaaaat tttaatttta aaataatatt
     9541 caaattaaac aactggcgta tctgctcctt
//
LOCUS       DACDNO010000085         9485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDNO010000085 DACDNO010000000
VERSION     DACDNO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.085"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            125..1429
                     /locus_tag="IWI22_002486"
     CDS             125..1429
                     /locus_tag="IWI22_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9169819.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1521..1886)
                     /locus_tag="IWI22_002487"
     CDS             complement(1521..1886)
                     /locus_tag="IWI22_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9169820.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1883..2248)
                     /locus_tag="IWI22_002488"
     CDS             complement(1883..2248)
                     /locus_tag="IWI22_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9169821.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2440..2688)
                     /locus_tag="IWI22_002489"
     CDS             complement(2440..2688)
                     /locus_tag="IWI22_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169822.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2687..2815
                     /locus_tag="IWI22_002490"
     CDS             2687..2815
                     /locus_tag="IWI22_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9169823.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2872..4566
                     /locus_tag="IWI22_002491"
     CDS             2872..4566
                     /locus_tag="IWI22_002491"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9169824.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4648..4839
                     /locus_tag="IWI22_002492"
     CDS             4648..4839
                     /locus_tag="IWI22_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169825.1"
                     /translation="MDLLPQMQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKVV"
     gene            5217..6350
                     /locus_tag="IWI22_002493"
     CDS             5217..6350
                     /locus_tag="IWI22_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9169826.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6381..6701
                     /locus_tag="IWI22_002494"
     CDS             6381..6701
                     /locus_tag="IWI22_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169827.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6730..7194)
                     /locus_tag="IWI22_002495"
     CDS             complement(6730..7194)
                     /locus_tag="IWI22_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9169828.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7323..8572)
                     /locus_tag="IWI22_002496"
                     /pseudo
     CDS             complement(7323..8572)
                     /locus_tag="IWI22_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(8601..8918)
                     /locus_tag="IWI22_002497"
     CDS             complement(8601..8918)
                     /locus_tag="IWI22_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9169829.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8944..9264)
                     /locus_tag="IWI22_002498"
     CDS             complement(8944..9264)
                     /locus_tag="IWI22_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9169830.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
BASE COUNT         3055 a         1870 c         1691 g         2869 t
ORIGIN      
        1 ccccttttaa ttagatttct agtctaactt ttggggtgca catcatttat gtgggcaatt
       61 ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg tttgcaaaac
      121 cactttgctc aatgatatca ataacataaa aaaagaattt gaagaacaga tcgcttatac
      181 acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc gatgcaagat
      241 catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt atttggaaga
      301 agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca gcaaagctta
      361 ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa tcgaccgcta
      421 ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt cgtatcgttt
      481 aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg atcgtttcat
      541 tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag aaatcttatt
      601 gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa tagattcaaa
      661 atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag catgggaaaa
      721 cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga cactattcaa
      781 tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc aacagctaca
      841 ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct ttgaatctca
      901 cttcaatcaa gaattattcg gaaaccaacc attcgaacgt gcgctgattc atctgatgcg
      961 ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttc taaatgagga
     1021 acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca gccagttacc
     1081 ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat tgagcggaat
     1141 tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg atgttcatta
     1201 tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag ttgcaccaac
     1261 aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa accgggtatt
     1321 atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta ttcctatttc
     1381 gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag ggactgtgac
     1441 aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac aatctcttga
     1501 tacacatcag gctcacaacc tcacttattt tggacatcta cgggtccgat ctctttattt
     1561 aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa aatatcctgc
     1621 aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc caaacttaga
     1681 agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg gtcgctttgc
     1741 acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca aacaccccaa
     1801 accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcaaa atatggaaga
     1861 agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt tctttgtctt
     1921 ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata agtgcaagct
     1981 tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca acgactagta
     2041 cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca ttacgagtat
     2101 tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat agaacaagaa
     2161 cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg atccattcta
     2221 gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta atttgttcct
     2281 attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt tctaatgata
     2341 acggttcgct taaatttttc tttttcactc tccccttttt ctccctccat ccataaatcg
     2401 tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca tcagcttttc
     2461 aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag aaccatacac
     2521 ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat cagacacttc
     2581 ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt ctaataaaaa
     2641 cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga gtaaaaaatt
     2701 aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc taggaaaaaa
     2761 gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa tctagcatcc
     2821 tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac aatgagaaaa
     2881 agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt ttatttgatt
     2941 caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga tcacggttca
     3001 aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa ccatccatta
     3061 gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg tacctatgta
     3121 gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat gactgctgaa
     3181 gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg tttgatggaa
     3241 ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga ccgtattctt
     3301 ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa tatcactatc
     3361 gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt tatgtgggaa
     3421 acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta tatgcatcaa
     3481 atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac acgttacaat
     3541 caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg atgcaaaatc
     3601 attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca agatgaaatc
     3661 actgtgactg gtttgaaaat gaccaataca ctgactcaag aagaagaaca cgttgtggtc
     3721 gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc tactttagga
     3781 gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag tctctggaaa
     3841 aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc tgatcgagat
     3901 gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt gaatgaaatc
     3961 acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc aaccacaccg
     4021 atcacgccgt taggccaaaa agatgtcaat atgtctatcg tcgtgcatca tcaaccgcat
     4081 tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta tccaagacga
     4141 cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat gctagaagaa
     4201 ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga aaaagaaaca
     4261 gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc ttccgcgttg
     4321 ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc gaccaatctg
     4381 gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga acaaagtgct
     4441 gtacgttctg gagaaatcgc tgctttccat tttgcgggta tccctatgtc acgtcttgtc
     4501 aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa aaaaatgttt
     4561 gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac aaccaggcta
     4621 aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca agcttttgaa
     4681 caccgtttac caagaggttg tgaaaaagct gccctgcatc aagtaataag agcagccaag
     4741 ctaaaacagc ttcttatgtt tttgacttgg ttggacttat taccgcagat gcaagctttt
     4801 gaacaccgtt taccaagagg ttatgaaaaa gtcgtttagt ttcgagtatg aagaaaaatt
     4861 ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag aaactgatcc
     4921 cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac cattttttca
     4981 acgtttactc tcttgccaat ccaacatcat ctaacagata caactatttc aatgattacc
     5041 attacactaa aaaatcggaa actcacctct ccgaacacaa atacataaga ctatagaatg
     5101 tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt taaaatgaaa
     5161 ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca tctccaatga
     5221 aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat gatccaaaaa
     5281 tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc ggctccgatc
     5341 tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta ggacacgaac
     5401 caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa gggcaacgtg
     5461 tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat cacatggaaa
     5521 gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga tttggcaagt
     5581 tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat gcagactcta
     5641 cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt ctatcagata
     5701 ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc gatacagttg
     5761 tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt atggctggag
     5821 cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg gctaattaca
     5881 atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa ttgtatgaac
     5941 tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga ttagagcctg
     6001 tcaaagaaaa agcgaaaaat ttagtaagct tacaaaacgg cacaatctct ccattacaga
     6061 tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt tatatgactc
     6121 cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc aagcatggac
     6181 aggcaccaat tatccatcta atgccaaaaa tttacgaaat gattgcagaa ggactattcg
     6241 atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa gcatatgaga
     6301 tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga attcagtaaa
     6361 aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag atcgaaatag
     6421 gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc caaaatcgtt
     6481 acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt tttggaagcc
     6541 ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg gctggtgcta
     6601 ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt tacgacgaaa
     6661 aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agatttgaaa aaatctcatg
     6721 cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc cactttcttc
     6781 cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat gatctgtaat
     6841 taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg ctaatatccg
     6901 cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa gatttggtgt
     6961 caaatcaggg cggttatgga agtcgttttc aataacgccc gctcctccga cgatggtttc
     7021 tttttcatct aagaccacat accattgagg aatactttcc ttattagcga ttccagtacg
     7081 aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc acttcgcact
     7141 ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa ccaatgttta
     7201 aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga aaattagcaa
     7261 tgacaacaat agtgatgtaa aaaaaacgga aaacaatcac ttgttctccg ttttttgatt
     7321 aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt gacgaacggt
     7381 agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc tgcaatactt
     7441 tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat aacggtagct
     7501 gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac cattggtgtc
     7561 aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa catcgttggt
     7621 tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat atatggtttg
     7681 tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc tgttcctcct
     7741 aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct atgtccagct
     7801 gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat caaaggatcg
     7861 accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa tgcaagcagt
     7921 aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac tgtatagaaa
     7981 gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa agcaaacgca
     8041 gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc cggtggcaca
     8101 ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa gatttctgtt
     8161 gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc taatgctccg
     8221 cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc ttttcggtag
     8281 ccgttagcta aatgataagc aagaaagcaa tcaagacaga aagaatggca aatgtaccgt
     8341 tccaagcagc agccccgaat cctttccatg attcaccacc aaaaattgag ttcatcaatt
     8401 cttggaaccc gggaatcggt aagttattga tcattactgc taatgctcct aagatcatta
     8461 acggcatact caccataaat gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt
     8521 ttgatgctac aggtataaaa tgtttctcca taaatgcaac aaatttatcc ataaatcttc
     8581 cctcttttct ttactgtaga ttacgcgttc aatcgacgat acagatcaat gatctctttt
     8641 gctaaatctg taaaagctat ggatgtcatc agatgatctt gactgtggac tgtcaaaagc
     8701 gtgacttcaa cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt
     8761 gcttggacta aagattcctc tgcatctttg attttttcat ctgctaaagt gaaatcacct
     8821 tttttcgcag cagcaatcgc ttccattgca tcgctttttg catttccgcc aaacatgatc
     8881 agacccatga tggcttctag atttttttga tcttccattt attgtatcct ccgataattt
     8941 ttattatttg tttattagtt tttctgcttg gtcgagtact ttttcaccat tcatcatgcc
     9001 atagtcagcc atattgatta tatctaaagg aatgccttta ggtgctagct tttcagcaaa
     9061 ttgattttgc ataaagcgta cttgcggtcc taaaagtaaa acgtctacat ttttagattc
     9121 tagttgatta tccgcatctg aagcagatac agcaaagatg tctgcttcca ttccttttgc
     9181 ttctgctgcc ttttgcattt ttgtgactag gagacttgta ctcattccag ctgaacatac
     9241 aagcatgatt gttttctttt ccattttaaa cacgttcctt tctatactta cacttgtata
     9301 taagcaagta gcgtgccaac tttgaaagcg ctttaaaaag ttgatatcat cacatttaag
     9361 gtctatacca agttgcatac acactaaaaa agaaagtgca cactaaacac acaaattaat
     9421 ttttattacc ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca
     9481 acttt
//
LOCUS       DACDNO010000086         9346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDNO010000086 DACDNO010000000
VERSION     DACDNO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.086"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(162..3803)
                     /locus_tag="IWI22_002499"
     CDS             complement(162..3803)
                     /locus_tag="IWI22_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169831.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVVYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMIDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(3806..5755)
                     /locus_tag="IWI22_002500"
     CDS             complement(3806..5755)
                     /locus_tag="IWI22_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9169832.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(5797..6855)
                     /locus_tag="IWI22_002501"
     CDS             complement(5797..6855)
                     /locus_tag="IWI22_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9169833.1"
                     /translation="MKKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(6974..7429)
                     /locus_tag="IWI22_002502"
     CDS             complement(6974..7429)
                     /locus_tag="IWI22_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169834.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(8012..8815)
                     /locus_tag="IWI22_002503"
     CDS             complement(8012..8815)
                     /locus_tag="IWI22_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9169835.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         2713 a         1927 c         1339 g         3367 t
ORIGIN      
        1 taccagcata agcccttttt aattatacaa ctttgaacag taaacattta attcatttat
       61 agacaattta aaataatttt tttgcataaa tagaaatttt ccttgacatc ttcagttaga
      121 aatcaaagta aaaagaagga gcaaatcaat gctccttctt tttactcgtc ttcagactta
      181 ttttcagttg atttttgatt accgtatttt acaatatagt ccttgactga catggtagat
      241 ttcttcacta ctttaccagt tttggatgct cctgtagcag tagccttggc aaccttagcc
      301 gttcctttac ctattttaat agcttcgttt ttagcttcat aggttatccc tgctagacta
      361 ccttttggag cttttgctaa agctttatct gctgaggtgt aaacattttt accagttttt
      421 gatagatatt caacggctga tttagctgtt gatggtttct tggttgattg attagatttc
      481 cctgaattat ctgaagtaga tttagaatta tttgttctat tatttttatt tgtcagttca
      541 ggttctctaa ttgattgatt gtctttcgac attcctgatc caatatttgt ttcatattta
      601 tctgtttgat taacattagg agtaggaatg aactttttct tcacatctga ttgttcttta
      661 tccgtttctt ttgttagcga ttctctgtct ttaagtgctg tgtcattccg aatagcacta
      721 ttatcaaagt tgctgttaag ctctcttgac tgactttcac cagatgctcc atctcctaat
      781 tcccgatgca ctctgtctga cacgtcctca ccactcattt cattcctcag ttcttgatga
      841 gtttgatcta aagtaatctc gtcatcttca ttctcaggaa ttgcaacagc aattggcgta
      901 tgccgttgtt gttcgagttc tctatttcga tctgccacaa gcgcatctct cgtctcaatg
      961 atattcccat cctcatcgac taaatctcca ttcacattat atccatcgct gtgtttacca
     1021 agtttattac caaactcatc aacaagattt ccatcttcat caactaaacg acctttttcg
     1081 tcaactaaac gtccgttact atcctgacca aaatcaccat ttagcaacct gcgtccattt
     1141 tcgtcaacaa gatttccatc ttcatcgact aagcgacctt tttcatcaat taaacgtcct
     1201 tcgctatcca taccaatatt accatcttca ctatcttcca atggtttacc attagtatct
     1261 ataaggtttc catcttcatc gaccaaacgc cctttttcgt caataaagtt gcctttagca
     1321 tctttaccgt atttactatc ttttaatatt tcaccatttt catcgacaaa gtttccgttt
     1381 tcatcaatca tacgtcctaa atgatcaata tattgttggg agttattacg tagttgatct
     1441 tgacgaacta aatctttgaa agaggtatta ttgattgttg aaccaactcc gccaccgttg
     1501 aatgacattc cttctggtgt ttcaggaatt actttatcag caatggactt agcatcagca
     1561 ttgctatctc ccatattgtt caaacgttca gccatttttt gcgctccaac tgctccaaga
     1621 cctcctgcaa ccatatcttt tactgagctg ttgccaccat cataataggg atttttagct
     1681 ccttggcttt tcttgattgc tttagaaacg ccgtccacaa ctcgcacagc atcatttcta
     1741 ttaccaaata ataacttagc acgttcatca aacccaaacg ctacttcaag tggtaagcca
     1801 ataagcatat taatcaaatt atttactttt tcgccttttt taaagactaa aagatatcct
     1861 acacctaaaa tcatagcaga tcgaacaata tttgctacgc catcgccaac taatgtatta
     1921 ggaatgaatc ccacagcagt atcaccaaat gcacgaataa aagtaatcag aaagataggt
     1981 aattgtagtc cgatcagaga tgttgtaaca acacctaatg aaattgccat cacacctgct
     2041 cctgatgcaa gtgatcctgt tagaactact tcccaaatcc gaataaattc acgtttaaat
     2101 gctttcacga tattcacatt gaacatcgcc caagcacatg ctaagtaaag aactgctaaa
     2161 acgattgatt cagcgttcat cgctagccac gagccaaact tacccatcgc tccatgacca
     2221 actaaggatg ctgtacgcca ttctgttttt actgttgaca tattcttagc actgccttga
     2281 attcctgaag gagcgatatt atacgcataa aacttagctc ctgatgtatc aagtgctgat
     2341 tggagcaata aagctaccga ctgtgtgcta aaagttgcat tgttagcaac gccccaaggt
     2401 tgagtagcat caaagttatg cgctgttcct tttccatcag gcttgcgatt gtcgtctgtc
     2461 acattttgat ttgctgacca aatatatcca tttaaattgg ttggtttctt aattgcactt
     2521 ccttcttttc catcccctaa attatagcct gtccaaacag caggcgctct tcccatataa
     2581 gcatcatttt cggcgacatt aacatcaccg gcataagtat taatattgaa agtatcattc
     2641 gatgaccatt cttttaatag agccataccg actgaattag aatctagatc attattttgt
     2701 ctggcataac tgctagtatt gatcgctctt atcattgcag attttttaga tggttcataa
     2761 tctgtatcaa cgaacttgat ttcggatgta ggctttccgt caggtacatc accaccagta
     2821 tcagatgatg gagcaagatt ttgagtagaa gcccaaagtc ttgtgttaat gatgtagtca
     2881 gaaacaacat ttgttggtat atctccatct ttttttgctg actggatgcc gttgatataa
     2941 actcccaata gcgatcctgc cactggtaag aatataaaca atacaaaaat acgaacaatc
     3001 catttttcaa gtactggtga taatcccttc gagttaacac catctttcga caataatttc
     3061 attccttgat aacctagtat tgctgtgaaa aagagcatag ataagcttgc tatcgcttta
     3121 aataattcat tatttaatcc caaattgtta agcaagctag ttaatgcttt agataccgga
     3181 ttagatacat ccgatccacc ttctccccac cccataagag aaaatatatt catactcatg
     3241 aaaaattttc caaggagttc atttatggat tcagcaatat tagaaaggat taaactaatt
     3301 gatccgataa aactaccaat cgtcctacca atacttccta gaatggatga tgcttgactg
     3361 gcaatagcat ttcctgagac tgtttcaata tatccgtagt aactataagt tgctaaagta
     3421 gatgctagtt gagtacctgc attaccccct gcaatcgaat taccttgttt agaaatgtca
     3481 gaataaggga aatcggcact gataccacct tgtcctaaca aattcatttg tccagtttta
     3541 gcggcactat cattaacttc tttatctccc attaggatac gagcatatag tgtccacatt
     3601 ccttgttctg ccggtttact ttccgcatat acaacgttct tatttataac tgtttcttgt
     3661 ttgctatctt gattgtcagg agttggatca ttcattccaa aaggagcgcc taaagtaact
     3721 gctaatactc ctccaataac tagacctctt acgacccatc gttttattct aaggttttgt
     3781 agtaaatatt tcttgaattt catagctacg cccctccaat cgaaaataca atcttgtttt
     3841 ctttactttc agcaacggaa ggctttgcaa taatttcagc attagaatca ccttttcctt
     3901 caaacgctcc ggcttcactc aacttaacat cgaatatttt tgcgtttgga taagtaatta
     3961 ttgcttcagt aatcgcttct tcaaactcag attcaactaa atgcaagata ttccttgcac
     4021 cccctgattt agaatcctct tgtgaagcca tgtcaaaaac taagtgcatg gagagatcgt
     4081 ctgcataaaa attttgacca tatactcttt ctttcctagg tggaagacgt aaatctatat
     4141 ttaaatgttt tcgtactaat tcagcactct tcaacatttc attataagca atttccagaa
     4201 gagtttgttc ggataaagcc caaaatggaa ctattcgtcc aaatcggtta ataacttcag
     4261 gctttagacc ttcaccaatc aaggcttgtt tgatccctga tgcgactttt cggtattctc
     4321 tttttagttc agtgctatat tcattagaat tttccttgtt tgtaacacga gatagtgagc
     4381 tatatctgtt taacgaatcg aacatctccg cacctgcatt tgttgtcgca aataaaattg
     4441 tatttgacaa tgaaacggtg tcgtgaactt ctcgatcatc ccatcctctt tcagtaaaac
     4501 ttatcctacc ttcatctaaa attgataaaa aggcatcgag tgtttctaca ccagctttct
     4561 catattcatc acacacaata atcgcagaag gatcatgcct tacatgagta cctatgagtc
     4621 gtctaaaacg cattgcatca tcttttgttt tgtagtccgg catattaact aagagaactt
     4681 tattttcatc gccataaagt cctcttgcca cggctttagc cattgcagtt ttaccgaccc
     4741 ctgttgatcc tgtaaacaga gcagttgctt ttggttgctt tcgaccttta aaacgatcaa
     4801 atccaagctt catacgttta aacaacattg ttatagtata aactgctaac ggttgccctt
     4861 ttatggactt taattcctct tgaagttttt ccatatcaaa atcagaatct agctgaatat
     4921 tatacttacc aaatatttgc aatagacttt ctcgattata agcatttccg tctactcgat
     4981 cttttgcaat caaatcctcc attacggcaa ttgatttagc cggttcggac aaatcctcac
     5041 gataaagctt gtttgcttgt acgatttctt gaagtaattc atcaggaatc ctttgcgata
     5101 atttaaaaga agcctgagga tcatctttaa cagaggatgt atttagccat tgtctcaaaa
     5161 tatcgacgac aacatcactt tttagttcat tcaattctaa cggttgtaac cgtctcccta
     5221 aggcatcatc tcttttgata tagttttcat attcttttct tgtagtagcg gtaatcaatt
     5281 gaagtgattc atttgctaac ggttctttta gcgaatcacc cccaatttta ttgccactac
     5341 caaaagagct aacaacttta tgaaactcgt caatgaagag gacaaattca ttttctttgt
     5401 cctcagattt taaaattcgc tcatattctt tcaatcttgg aattaatgtt tctaaacgtg
     5461 tttggaggac atcatctccc ccaacacgca attgtccgat tgataatttg tatacttgta
     5521 ctttctttcc atcttgtcgc tgtctttcag cccattcact tgcaagtgct gttttacctg
     5581 ttccggcttc acctaacaag atagcttgcg gtgtttccgg tctattcaag atcagtgaca
     5641 actcatggag ttcgtcatca cgtcccacta acttatgttt cggtggtttc attggtgtga
     5701 gagcatgatt aaattcagtt tgaatattct taaaaaactc ggtactattt tccatctgtt
     5761 tgtgttcact tcctctaaaa taagaataat ttacttctag tcagtcagag ttaggtactt
     5821 aaatgtcgtc ttcatgtctc caaaagaatc tttgttaaag tcagcatttt tgtattgatc
     5881 aatcaatttt tgtgtatcat tgattgcttt aatttcggtc tccattcgac tgtctagttg
     5941 acgaatactt tcttgcgaat ctttatcgtc aggattttgt tgtaaacgtt ccagatactc
     6001 agcttttgta gcttcaattt tcttgatgtt agtatttgaa gcatttaact gatcttctag
     6061 cttatttaca acattattct tacttgtaac tgctacaact tttgaataat caatatcgcc
     6121 atctttggta agaaagcttc cttgatagac attagttgaa tctgaatatg gatttattct
     6181 gaaattgata ttatcaggag tattaacagg ttttgctttt ttcccaaaag aaattactcc
     6241 ttcattatta acatcattca tagaatattg agaaagtgct tttgtaactg aatctgcttc
     6301 attcacattg actttggtat catcatcagc tgtatcatca ccagaaatta gtgatacttg
     6361 gtttgcaata aaaacttgat aagctctttc atctaattta ccttttagga ataagaaagc
     6421 atccccatca gtacttaaca ttccatagct catttcaagc ttcttaggac gatctttttc
     6481 ttttgttgcc atatataagt tgtagttttt tccttgcgta gataacaact ttctagcgtt
     6541 ttgcgagtaa ccgagtttta ctactgttac atctctgttt tgatctgtcc acacatctga
     6601 aatagtgatc tctgctcctg atcgagcaaa ctccaatgaa gagcctatag gtgttgtatt
     6661 gttcacgaga taataattat agtttcctaa ttgataaatg tagctaccca agcctgttaa
     6721 taaaaaaagc actcctagaa aagttgcaag cataatgcgt aatccgtgac gtgttctctt
     6781 atccttatca ccaagcaacc ctccaataat actgtattga tctttggtag atgttgaatt
     6841 gacgtttttt ttcattagtt tgtccgcccc ctttcataaa agagcaaaca atcagacgtt
     6901 ttttcggtaa ttctaggttc gggattttta atcatttcgg tatattcttc cccattaata
     6961 tttaacacaa aaattatctt cctttcttcg ttcctttctt ttgctcttta ttttccaaat
     7021 aatcaatgat ttttgctatt gtaccaagat agtaattccc ctcggctttg ttttttcgga
     7081 ttttgctaac cattggagta ggtacaatct cttcatctct caattcactg aaagattttt
     7141 tacctagata ttttccaata acagatttta aaaaacgttc aaaatctttc tcgtaagttg
     7201 ttttcttgcc ttttagttca tggttcatat tgtcaacgac ttttgcggtt tcaataattg
     7261 ttttagttct cagcttaata atttcatctg gaattttcaa tcctcgtaat ttcatcgttg
     7321 tactaactgg taacccatgc tttactgcat cagcaatact aaaacttttt agatcatcta
     7381 gtaagctctc catatagtta acaaattcat gccaattaat agcattcatt gtctcacccc
     7441 ttcatttttt taaaatgagg ttcgggcgta acacatttat gttattataa tatcataata
     7501 acataaataa atcaatcagc aaatttagat atcccttaaa gtatcagcaa ttattcaaca
     7561 agatatttat tttagctatt tcagtcatca tgaaagacta attagcaata aaataactaa
     7621 tattatccat ttatcagacg aaaagaaaaa acttatctaa ttactctata ttaactttta
     7681 acaaatagtt gacttgtcca aaaatgttac cttcaatctc acaaagatat taagatgttt
     7741 cctaccaaaa ctgatatcac cggacttttt attctgtgtt gtccaaaagt gttaccttta
     7801 gtcgatcaac aaattaaaaa aattgttttt ttcttgactt gtccaaaagt gttacccata
     7861 gttgttaaaa tttgtataga ttagccattc tttcaaatat tatttagact gaaacttgct
     7921 tacaaaaaaa cttcttgcat ttattgtcca aaagtgttac ccatactttt ttaaagaagc
     7981 aggttgtatt aaaattacaa cctgcttctt ctcaatcttc ttctaaccaa ttatgcattg
     8041 aaatggttgg caagtttgat tctggctcac taaaagtgaa tctgagccga tcaactcttc
     8101 ttcctttctt tgcatagatt ttttcataat caaaagtttc aaatacctgt attccgtttt
     8161 catcaggagc taaaaattct tctttcgcct gctctagtat tcttttgtca atatttgtca
     8221 ttctataact tgaaggtata cttaatgctc ttctaaaatc ttcaactttg actacccaat
     8281 aaccatttcg ccactcttta tttctgtatt gcatgagatg acgatacaat tcttttgtgt
     8341 agctacttct tagttttgta aaattatcaa gttcaaaacg agtaaaattt ttgccaattg
     8401 agtttaagat aaattcaaat tcctcatttg ccttaattgt aactgtttcc ttctgtttat
     8461 ctatccggaa tttttgaaaa agccacagct gttcatagac gtctgaatct tcaaaacggt
     8521 aggttaaagt acctagtttg tcactcaaag cttttaatac agaataaaaa ctgtcctttt
     8581 ctcttctgtc gtatttgacc aaataacgaa agaggttgaa atcaaaagtt acttctgctg
     8641 ttccttgacg tttcattttt gataaaatag cccaaaacac atccatttca acaggcttaa
     8701 atcctcgcaa agctgttgta ttcagaccat tgttataacg cacaattgca tttgtttgtt
     8761 ctgatactaa atctacatca ctatcaataa tcttttcggt aacttcttga gacattgatt
     8821 actctccaat cttctttttg cgctccttta atcatgttaa tctggtacta attaaaggaa
     8881 acattttttt ctgcttgtta cctaaattaa atcacaacta aaggtaacag tcaacaaaga
     8941 tttgttacta atctaatttt ctattttttc gccttccaaa tcaagaaaac cgaaggtaac
     9001 gtcttatcct agtatgatac ctcaaatgtc cacttttgtt acctttgctg tccaaaagtg
     9061 ttacccttac tgtccacttt tgttaccttt actgtccaaa agtgttacct ttatacccct
     9121 ttaatactta ctctcccaag agattaagcc tcccctaaca gatatattaa cagatattta
     9181 acagatatat taacagatat ttaataaagg aggattactc ctctcttatt tcaaaatctt
     9241 aaaaaaaaac taacactatc taggagttta ttttgcttgt gtaactttga ataattgaaa
     9301 aaaaggaaaa ggaggaagct tcagcttccc cttttattta tattta
//
LOCUS       DACDNO010000087         9286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDNO010000087 DACDNO010000000
VERSION     DACDNO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.087"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(189..1556)
                     /gene="nhaC"
                     /locus_tag="IWI22_002504"
     CDS             complement(189..1556)
                     /gene="nhaC"
                     /locus_tag="IWI22_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9169836.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1665..3086)
                     /locus_tag="IWI22_002505"
     CDS             complement(1665..3086)
                     /locus_tag="IWI22_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9169837.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3329..5206)
                     /gene="tdc"
                     /locus_tag="IWI22_002506"
     CDS             complement(3329..5206)
                     /gene="tdc"
                     /locus_tag="IWI22_002506"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9169838.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5497..6753)
                     /locus_tag="IWI22_002507"
     CDS             complement(5497..6753)
                     /locus_tag="IWI22_002507"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9169839.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7333..8634
                     /locus_tag="IWI22_002508"
     CDS             7333..8634
                     /locus_tag="IWI22_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9169840.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8703..>9286)
                     /locus_tag="IWI22_002509"
     CDS             complement(8703..>9286)
                     /locus_tag="IWI22_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9169841.1"
                     /translation="RAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVSKATIFK
                     YFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRVNKQFYI
                     DLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLILYFQAM
                     VEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         2810 a         1928 c         1659 g         2889 t
ORIGIN      
        1 aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
       61 cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttaaaaagc
      121 aattttcttg agtttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt
      181 tccttccttc aattttttac acgtcgacga ttcaataaaa aaccgcctaa tattgtaaag
      241 acaggtgcaa gaatcgagaa aaaggcgaat ggcaagtaat gcaacgcgct aactttcatc
      301 gttcccataa tgaatgtgcc gctgactccc cacggtacga gagaattgac ggtcgcacct
      361 gaatccgcta gtgttcgtgt caaataggtt tttgataagc caacgtcatc aaaagaacgc
      421 ttgaatgttt ctcctggaag aatgatggac agatactgtt ctccaacgat cagattgatt
      481 ccgatacagc tcaaggcagt aaacccgact aaacgagaag gtcggtccat cttttctttt
      541 aattcttgaa cgatggtatc aacgatcaga tatttgatca gtaacccacc taatgccaat
      601 gcaagtaaaa tcaaactagc agaacctaac atactcatga tcccgcctcg agaaagcagt
      661 tcatctagct ttggatcagg tgtatttgcg acataaccat tcatcaagat gtctgctgct
      721 tttccgaccg tcaaactttt ctcatggata aaagaaagaa cgagagcagc tgtcgatccc
      781 gctagtaatg tagggatagc cggaattttt ttgattgccc ccccaaataa aataactagt
      841 ggaatcaatg tccaaggaga gatccaaaag ctcgctttca tgttttggat catttcattg
      901 atactagcta gatcggctga atgactgtta tgtcctaata tcgtataaaa aatcaacgaa
      961 aggacgaatg ctgggaaatc cgtataacgc ataccagcga tatgttcgaa tagattaact
     1021 cctccaatac ccgctgccaa gttggtcgta tctgataaag gcgagcagtt attccctaaa
     1081 aaggcaccgg aaacgactgc gcctgtagtc attgcatcat tgaatccaag taaatgaccg
     1141 atccccaaaa aagcaattcc aatcgtcgaa atcgttgtaa atgaactgcc gacagtcact
     1201 ccgacaattg cacaaacaag aaatgctgtc ggtaaaaaga aacgcacaga aacaagttta
     1261 aatccataaa ccataatcgt agggatggtt ccagataaaa tccatgaagc aactaagaca
     1321 ccaatcaaaa gaaaaatcag gattggaatg atccctggag tgattccttt gatgatcccg
     1381 tcatggatct catcccatgt gaacttttta aatctgccat aaaacaacaa acacatgaaa
     1441 gctgcaagga tcgggatatg tggcgagagc tggaatccga tgatcagtac tcctaggagt
     1501 gccaacaaca cgatcaatac agcgatactt tcttttaaag aaatattttt attcatcgtc
     1561 gatctcctat ttcaaatgaa aaggccctgc catgaaaaaa ctattcactt ggcaggacct
     1621 cctactaaat cagcgacaaa acgtcggctg aaagacttat ttttttattt ttcttctaaa
     1681 cttttttcta cctcaatccc ccaagaatct ttcttgaagt gattgatgat caatggaata
     1741 acgaacatga ccactgtgat agctgctact aataatgcat cactcaatgt cgctgtacct
     1801 aatgtagcaa atacgacaac gatgatagag aagatcagca tgcagctgac tacccaagct
     1861 aatccgtttc cgtttttacc aattcggtaa ggacgttcga attctggatg tttcttacgc
     1921 attttaagca atgaaacagc aatcaaaatg tacacgatac aataaataac agttgtagca
     1981 tttgtaagtg tcaagaatac agcacttacg tcatccatta caccataaag caaagcaaat
     2041 aatgaaataa cgattgattg tgttagaacg acatttctag atacgccgta tttgttttct
     2101 ttatggaatc cgaattttgg tggaaggaat ccttcacgag ctacttggat gattgttttt
     2161 gatggtccag taacccaagc tgataattgt aaaagcacac caataaatac catgaagctg
     2221 aagatatttc cgatcacttc tggtaagcct aggatttgac agtcaatcaa gataggctgc
     2281 gtgatatttg ctaattccat ttttccatct ggtacgacat ttgctacaag catcgcattg
     2341 atgacattca ataaaactaa tccgatcaaa gcgataaata cgccttttgt ataatttttt
     2401 gttgcgtctt tcaaacgagg catgtaaaca gaactcattt cgatcccaac aaaaataaat
     2461 gtgataccag ctaagtattt caatgaatca atatgttcca agtcagggaa agcttttgac
     2521 catgaaaagt cacctagata gccgtttgag actagtccga ctttgaaggc tgccaaaaca
     2581 cctaaaacga acatgatgac tactgggata tataccccta gccatacacc gatgttcccg
     2641 ccgacttttg ccatatcgaa tttcaagttc aagatcgtga tgatccagta gataactaaa
     2701 atacaaccta aaacgaacca gtgattattg ccaagttcta cattaccaaa tgtgtttcct
     2761 aacaatggtc ctaaagtaga agcgaccatt accatacctg ggaacatttg cacccataat
     2821 agccatgcaa cgacaaatcc ccatttgctg cccagtgctg tttttaccca taactgagga
     2881 ccgccttctt gaggaagcat cgtggaaagc tcaccggaaa ccattgatat cggaccggca
     2941 aaaaacaaaa ctgcaaaaat tgaataaaaa atcaatgtcc aaccgactgc ggctaacgta
     3001 ggaatactcc gaaccgttgc acacagtgcc attgtcagac cgataaaggt cccaagaccc
     3061 attttactta cttcagattt actcaccgtt gacactctcc ttgatttttt ggataaaata
     3121 acggtgtctt tgaagtagtt tcagcaataa acgaaacaat tcagcgaata tttgagggga
     3181 ttctttgagt ttattgcttg aagggtttct tcaaagacaa cctcaaaaca aagggatcga
     3241 agagcttagg tctccccttg ctcttcagat ccaaacatgc gtcagaaaca gttagtattc
     3301 aagctgtttt ctaacatgat taccttgctt attttgcttc gcttgccaat aattggtctg
     3361 cgtagatttt ttccaatttt tcttgaagag ctgctttgat tttttcagca tattcttcaa
     3421 agtgttcttc tttgttcatg tatggtgtca ttacgctggc acgcaataca gttacttttc
     3481 cggcacggtt ccattcttca tctgagaatc ctagttggtt cacaaattgt aatgggctgt
     3541 tgccatagtc tgggatagca aagtcagtat gtgaagttaa gaactcatta ccgtaaatgc
     3601 ttcctttcac gtatgaagaa taatcatata cgtcatggtt caatttattc attgcaacta
     3661 agtcatcgtt gcctttttct ttgaatacat agtctaccat attgaaatct ggataagtta
     3721 atggatgaac ttcgatttct ttgtctccta ctttaaatga caagtcattc aagaagttgt
     3781 agaaacgatg tgcaccttcg attgatgcac ccattaattt accatatcct gtgacattca
     3841 aaggtaagac atggtgtgct gcccaaacgc tggctgctgt tgcccctgct tttgaacctt
     3901 ccaagatata agccccaagt aatgctggga tatctgcgcc tttttcaaat acatatgtcg
     3961 caaagtaaga gataacgtct ctcatgcgga tatctttgat gacgatacca ccggcagaat
     4021 atggaacata gcccatttta tgtgggtcaa tcgttacaga ttctgcttct tcgattgctt
     4081 tatacgcact gtgtacttct tctaagatat aatctttgtt ttctgtaaag acattgtatt
     4141 tgtaatgaac atcttttaaa tcttcaaatg gaatgaagtt gttgtcttcg tctaagaaaa
     4201 tcgcacggcc gtatccgcca taagcagcat ctacgtgtaa gtagaagtaa ataccgtctt
     4261 tttctaatac gcgacgcaat gcaacgattt tatcgatacc gtcgattgct ccttcttctg
     4321 ttgatcctac aacaccgact acacctagga ttggtgtttt ttcagctgct agaccacgaa
     4381 cgattttttc aagttcgttg atgtccatgc gatagttgtg gtcaactggc actggaatca
     4441 cttggtctaa accgataccg atgatgtcgg cagcttttag ccatgaatag tgtttagttt
     4501 gtggaaccaa ccattttcct aatttttcta aatgttttcc gctacgtgct gagtgagctt
     4561 tgatttcgtc gattttttct gggacgctgt ctaacagatt catgatttct tctgttgaca
     4621 agttcattaa ttcccaatcg ctttttccag caactagttc aggtgtcact tctttcattg
     4681 caagcggcaa tgatttgatg ttgcgggcat accaaagtcc ttctaagttt gctaaagaac
     4741 catcagcaac gatgtggccc cagccatctt tatagctcat taattttgca aattccattc
     4801 ctacttcttc ttccatttgg cttgttgcag gagaagattc ataggcaacg ttattaccat
     4861 tccatagcat agcaaaattg tacgctagta aagatggcat caatgtttca gagttcatat
     4921 gaccccagta acgtccggca ttatgccaag gaacggaatg ggtacgcatc cgcgctgaaa
     4981 tttctgatag aacatccttt gttttattca cagtgtgttc aaaactagca ctgctttttt
     5041 cttctggcgt aataataggc atgtcttgag gcatgtagtt ttgacgccag cctaaatgtt
     5101 catccaccaa ttcattcaac aaagctttat agatttggcc gttctcagct ttgtccccaa
     5161 tgaatagggc atttaggttt agatctttcg acaatgattc actcatagtg aaaccctcct
     5221 aaaaaaatat ttaaattttg tcgagcccta cattacacct gttcttctat tctgtaaact
     5281 aagttttggc ctgattttta atcatatttt aatatgtatc aattatccga attgttcaca
     5341 cttttgaaat aattaaaaaa aacgagggaa tttacaaatg taaactccct cgcaaagatt
     5401 gtattaacga caaccagcgt tctattttag gatgggtttc aaaattggaa tgttgtaata
     5461 gtatttccct ttttcaaggt aaaaacttac taaattctaa tcaattactt tggccaaaaa
     5521 gtaatttttc ttaccttttc gaataaccac aaacttacca tcgaattcat cggatggatt
     5581 tatgacaaaa tttaaatcgg taacgcggtc accgttaata cttatagctc cgtttgaaac
     5641 atcttcacgt gcttgacgtt tagatggttc aatttttgtg gaaacaagca gttccacgat
     5701 attttcaggt gtgcttgaaa tttcaacgtt cggcatttta ccgaatcctt gcgcgatttc
     5761 ttccgcattt agttctttaa tatttccaga gaacaatgct tgcgttattt tttgcgcttc
     5821 tttcaagtct tcttcactat gcacaaatct tgtaacttct tctgccaaac ggcgttgtgc
     5881 ttcacgtttc tcaggttctg tctctacttt tttcgctaag tcttcgattt cttcttgaga
     5941 caagaaagta aagaatttca agtatttcac tacatcgcga tcatcttgat tcaaccagaa
     6001 ttggtagaac tcaaatggtg aagttttctt cggatcaagc caaacggcac cgcctgcagt
     6061 ttttccgaat ttcgttccgt cagccttcag catcaatggg atcgttaaac cgaacgcttc
     6121 tgcttctggt ccttctaatt ttcggattag gtctaatcca gctgtgatat ttccccattg
     6181 gtctgctcca ccgatttgca attgcacatc gtatgtttta tgcagatgca aaaagtcgat
     6241 agattgcagg atttggtaag taaactctgt aaatgagatt cctacttcca aacggctggc
     6301 aacgatatct tttgctaaca tcgtgttgat gttgaagttt ttaccgtagt ctctcaaaaa
     6361 ttcaagtaaa gagatttttg acaaccagtc gtagttgttc acaaatgtga tatttgcatc
     6421 tttaccaaac aattttctca tttgattgga aagtgcgtcc acgttatgtt gcacttgttc
     6481 catcgtttgc aacacacgtt ctgttttgcg gccgcttggg tcgccgatcg ttcctgttcc
     6541 gccgccaatc agaatatatg gatgatggcc tgccaattgg aatcttttca tcatcataaa
     6601 tggaatcaaa tgtccgatat gcatcgaatc accagttgga tcaacgccgc aataaagtga
     6661 aatgctcttt tcttcaacga gctcgcgaag tctttcttcg tttgtttgct gattaatggc
     6721 gtcgcgccaa gttaattcat caataatatt cattttactg acctccgtgt agttttttta
     6781 tgaaagcagg aacctgctag catgtgtaat taattatttc ttcaggatag gataggaaat
     6841 aaaaaaagtc ccttggcata taagccaagg gacgaaatct cgcggtacca cccaatttgt
     6901 atgtctagat atccaacata cctctcgaac atttaacgct gtcgcgtggc aatcactgcc
     6961 aaagactcct gaaatgtaat tcgctttaaa gcatgtactg gtttgcagcg accaccagct
     7021 ttctgaaaca ttggctttaa caactacttc gtttcgatct tcgtcttgtt attattaata
     7081 tgtgtctatc atagagcgca actttttctt tgtcaacaga aaaaggatat tttttttaaa
     7141 ttcctcattt tttctttttt gaatccgctc taattccgaa cattttattt tcaattattg
     7201 ttaaaagaaa taactttttc tgtatttttt ctgcaattta tcgagaagtc actttacttt
     7261 gcctcttatt ctttcttcgt tacaataagg aatgggacaa gtaaaaaaat gaaaaagcaa
     7321 gggacgaggt atatggaaaa aaagcaaaaa attattttag gagccacttc tgctttaact
     7381 atcgttattt taattctaag tttgtttttt gtcatccgat catctacaaa aaaagaacca
     7441 atcattcctg aaaaaaaaat aacagaaaaa gcaaaagaac cgacagatga taagcaacaa
     7501 ctagttactc aagcaaaaaa tctggctcaa agctacgatt atgaccaagc gattgcgtta
     7561 ttggaaaaag atagtaagaa cggatctact gaaacacaac agcttcttca aacactaaaa
     7621 aaggagaaag aacaactgat cgactggtct gatcctactc agatctctca tgtctttttc
     7681 cacagcttga tcgtggaccc gcaaaaagcc ttccactctc aacaggcaca aggatacaaa
     7741 gattatatgg tgactgttga agaattcaac cgttcgatcg aacaacttta tcaaaatgat
     7801 tttgttttag ttaatttgaa tgatttgatc caaaaagacg aacaaggaaa tctttccttt
     7861 aacgggcttg cgctacctga aggaaaaaaa ccattggttc tttcacaaga tgatgtcagc
     7921 tattatgaat atatggataa ttccggtttt cctagccgat tggtcgtatc aaataacaat
     7981 gaaatcaaaa atgtctataa agagaaagat aaagaagaaa tcggtgactt tgacatggtt
     8041 cctctgatcg atgcctttgt caagaaacat cctgattttt cttatcgagg cgcaaaaggt
     8101 accttggccc tgacaggtta caacggagtt ttaggttatc gaacatcaaa atctcaatat
     8161 ggagacaatg aaaaaacaaa aaaagagatt aaagaagcta aaaaagtggc ggaacagttg
     8221 aagaaagacg gctggagctt tgcttcccat acatggggtc acttgaatat gactcaagct
     8281 tcactagaag atgtaaaaaa agacaatgaa ttgtggcagc aagaagttgc acctatccta
     8341 ggaaaaacaa atattctgat ttatccattt ggtgcagaca tcagcgactg gcagccttat
     8401 tcagcagaaa atcaaaaatt tgcttacttg aagagtcaag gtttcgatat tttctgtaat
     8461 gtcgatgctt ctacaccagc ttggggacaa ttaggtacgg atttctatcg aaatgctcgg
     8521 atcaatatcg atggcattcg ttttcaatct gatttagacg gaaaaaatcc tattcttgat
     8581 ccattcatca atgtccatga tgtctatgac aaagaagcac gcgaatctgc ttgattttac
     8641 gtcatttatg aaccaaaaaa gccgaactga cattttatcg tcagtttcgg cttttttatg
     8701 ctttatttct tcttcggtgc gattcccttg atcaggactt ctgtccattc tgctgtatat
     8761 ggaagcagtt ttgcgtatat ctccggctta gaaattcctt ctaccattgc ttgaaaatag
     8821 aggatcaaga actcatccga atatttcaaa tccacttttc cttctttccg ccctcgatgg
     8881 aaaagatcaa gcatgatact gaaactttct tgcgtatacg catccatcgc gtcagaaaat
     8941 tctttttttt ctttttttgt atagtatgtc atcaaatcga tataaaattg tttgttcact
     9001 cgattgagat aacggatttt gttttcactc atggcaatca gtgtttcttc aaatggaaga
     9061 ttttccgcaa tcaacgcttt cgcttcttgc cctaaatcat tcataaatgc aaaaaatact
     9121 tcataaatca gcccttcctt gctgccaaaa tatttaaaaa tcgtcgcttt acttacctga
     9181 gcgttctcag ctaccttatc cattgtcact ccagcggttc ctatgtctgt gttcatcaag
     9241 tcaaaagctg cttgtaaaat ctctttcttt ttctcttgtg ctctct
//
LOCUS       DACDNO010000088         8627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDNO010000088 DACDNO010000000
VERSION     DACDNO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.088"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(230..922)
                     /locus_tag="IWI22_002510"
     CDS             complement(230..922)
                     /locus_tag="IWI22_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9169842.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
     gene            963..1580
                     /locus_tag="IWI22_002511"
     CDS             963..1580
                     /locus_tag="IWI22_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169843.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQV"
     gene            1592..2893
                     /locus_tag="IWI22_002512"
     CDS             1592..2893
                     /locus_tag="IWI22_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9169844.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
     gene            complement(3057..3179)
                     /locus_tag="IWI22_002513"
                     /pseudo
     CDS             complement(3057..3179)
                     /locus_tag="IWI22_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
     gene            3292..4482
                     /locus_tag="IWI22_002514"
     CDS             3292..4482
                     /locus_tag="IWI22_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9169845.1"
                     /translation="MNYFTTSLMKNLFSNDNNLPAFEQAVTELFRSELEKSLNEILAY
                     ELTDFLDYEPYARTNNENSRNGSYQRKFDTKYGTITLNIPRDRMGEFFTSLLPKYRRR
                     DLFTETTILDLFEEGMSHSEICSMIQKLCGARYSKQTISNITDKALECVDSFKNRILN
                     KEYAVVYLDGTSMPLRRDTVSREMVHIALGITVEGTKEILGYMIAPNESAEIWEELLK
                     DLKSRGVERISLFCTDGLSGMENVIQDIFPGSKIQRCLVHIQRNICAKVRVSDRKEVA
                     NEFKEVYRSKSKEEALTSLKKFIDRWKHKYPSMVKSLSNNDYLFTFYDYPESVRHTIY
                     STNLIEGNNKQLKRSFKKKEQFPTEKSEEKYLVIQFNRYNEKNMNHVHRGFGQTTRED
                     WFKS"
     gene            4590..5123
                     /locus_tag="IWI22_002515"
     CDS             4590..5123
                     /locus_tag="IWI22_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169846.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLLHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(5249..5926)
                     /gene="nth"
                     /locus_tag="IWI22_002516"
     CDS             complement(5249..5926)
                     /gene="nth"
                     /locus_tag="IWI22_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9169847.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(5933..6631)
                     /locus_tag="IWI22_002517"
     CDS             complement(5933..6631)
                     /locus_tag="IWI22_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9169848.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6899..8083
                     /locus_tag="IWI22_002518"
     CDS             6899..8083
                     /locus_tag="IWI22_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9169849.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(8089..8532)
                     /locus_tag="IWI22_002519"
     CDS             complement(8089..8532)
                     /locus_tag="IWI22_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9169850.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         2577 a         1617 c         1485 g         2948 t
ORIGIN      
        1 tcaggaataa aaattatttt taataacaac ctattatcct ttccaagctt gtagatattt
       61 tggtttagat gttttttatg tgagttcaat tctacagcgg agggaatgag aaagttttct
      121 aaacaagttt ggaaattcct aaacatgttc agaacacaca gaaaacaatt acattctaaa
      181 gaagattctt tatactagat tttgaagaaa cagtggccac agcgttcttc tatttataaa
      241 tagccttcag cgccgcgctc aacaaaagct caaattgatt cagtgtcgtg ccaaagaaaa
      301 gatttggttg gtaatcagat attttttgtg gaatataggt ttccgtgcta atggttaatg
      361 ttgccagcgt accgattttt gaattttttt gcccaacaaa tgcgatcaca tcgataaagt
      421 gatcgttcgc aattttagct aattcggcta atcgaactgt atcaccagaa ttggaaataa
      481 taaaaagcag aatatcttgt tgcacttgct gtctaagaaa ttctaaatga ctattggccg
      541 tcgcacgaaa tccatataga ttaagagatt ccgaaaaata attggctaga ttttgcgaaa
      601 aacctgagcc aagaatcata atccgtttat cgcgatacgc agacattaaa tgtatgaatg
      661 cttccttctc ctcattggag aatgtgcttg gcatcgtaat gtcagattgg ctgtttccta
      721 gagcaaatac caactcacta taaccggaat atccaatttt cttcgccata ttgataatcg
      781 ttgcagtaga aacatacgat gctttggcaa cgtcacgaat cccaatattt tttaaagact
      841 gacgattttt tttcataaat cgtaagactt cagcttctga gtcattcagt tggtatttat
      901 ctataagcat ttcaacatcc ataagattca cctcaaattt tatctaatag gagtattata
      961 ttatgacaga aacgaaattt attcaagatc cgtggtcaaa aatgactacc cgtaacggct
     1021 tacgtggaga tgaagctatc tcagcattac agaaatcgat tcgtcgagga aaagaaaaag
     1081 aagcatgtga atttgcttat gaaatgtata tcacctcacc acaatttgaa gagaaactct
     1141 ggagaaggct attggcgatc agtgttgagg atgtgggcat gggcaatcct atggccgcca
     1201 tcatgatcaa taacttaaac gccatgcgca aagaattcca atacaatgaa tccgatcgtg
     1261 cgatgtattt tttccacgcg attcgttacc tatgccaatc tgaaaaagat cgttcatcgg
     1321 atttacttaa gaacattgtt atcaagagtt tcgctatggg atatattcca gaaattcctg
     1381 atattgcgtt ggataaacat actgttcggg gacaggcaat ggggcgtggt tcagaacact
     1441 ttttgcatga agcaagtaaa gtgatccccc aagcaaaagt tgataatgac tacaaggaac
     1501 aatacgaaaa aatcatcgag acctatgatc cagatcacgt cgtgccaagt acgttttcat
     1561 ttaatccttg gcaagtatag gaggaagtta tatggaaaat tctgcgttta tcatgaaact
     1621 gaatgacata cttagcccca tcgcagccaa gataggtaat cagcgccacc taaaagctat
     1681 atccagcggt atgatgtttg gcctaccctt tattgttatc ggttcttttt ttctgatttt
     1741 tgcaaatcca cccatcaata ttgataaata caatccagat acagctaata ttttcatgaa
     1801 atttatggcc agctggaaag attttgccgt agcaaattat gatctcatca cagcccctta
     1861 taatttgaca atgggaatca tcggattcat tagtgttttt ggcattgcat tttcccttgc
     1921 caatgaatac aaaattaatc cttccatgaa tggcatggtt gcatctgtca tttatctaat
     1981 ggtttgtaca ccagtggaag aaggtgccat caatcttaat ttccttggga caaacggatt
     2041 gttcgtcgca attatcatcg gcttgctagt agtggaaatc aaccgactat ttgaagtaaa
     2101 aaatattaag ttgaaactcc cagatacagt tccacccatg gtagcaacct ttatcaatat
     2161 gctcgttcca ctgctgacaa atatattcat cttttatggc atcaatctga ttttcttggc
     2221 agcaacaaac aaaatcttcc ctgaagcggt gatggaagtc ctgactccag caacgaacat
     2281 cgctggcagt ctaggtggtt tcttattgat cgtgactttg ggtaacatcc tatggctatt
     2341 tggtatcaat ggctcgtcaa ttattttccc tatcgtcttt acattgggaa tggctcaaac
     2401 tggtttgaat gctgaacaac tggcaaatgg tgaaaccatg acacacttaa tgaacttaca
     2461 aatgttccgt atttctgtac ttggtggagc cggtggtact ttgggtttgg ttttattgat
     2521 gatgcgaagc aaagtacccg aatatcgcac aattggaaaa ttgtcattgg ttcctggaat
     2581 ctgttccatc aacgaaccag tgattttcgg attacctatc gtttttaacc ctattttggc
     2641 aattccattt ttgattacgc ccatcatttc attattgctg acctactttg ctcagcaact
     2701 aggatggatt gggattggat ttatcgtcga tccttccttc acaccttttt ttgcacaagc
     2761 ttatctttca agtatggact ggcgcaatat tctcttttgc ctactgttga tcgtcatcag
     2821 catcttcatc tactatccat tttttaaagt catggagagc aataaaacaa aattagtaga
     2881 tgaaaaacta tagtcgtcat tcttaacctt gtaacgaaaa tatggttcta tactatttag
     2941 tgttgatggg aaatcttagg gtttcccatc aacctatttt ttataaaaaa gtatactctg
     3001 gtgtcccaca acaaataaag gaagcgccct tgttcaaaag gctacttcct ttatttttat
     3061 tttgctttaa attttgagtc atcatttata agcgaacgta agagataata gacacctaaa
     3121 aatagaaaga aaaataaagg taatacacgt agtaaaacaa atggagtttc aaaaataaat
     3181 ggatagcata aaatatcttg tgtaaattcg tttttctaga aatgtagaaa aaaaggtata
     3241 tccttagtaa aattagttcg accaaaaaca atttacgaaa ggaatatacc catgaactat
     3301 tttactacat ctttaatgaa aaacctattt tctaatgaca acaatcttcc agcttttgaa
     3361 caggctgtaa ccgaactgtt tcgttcagag ttggagaaat ctctcaatga aatactcgca
     3421 tatgagttga cggattttct tgattatgaa ccatacgcta gaaccaataa cgaaaattct
     3481 agaaatggtt cctatcaaag aaagtttgat acaaaatatg gaactattac actcaatatt
     3541 ccaagagata gaatgggaga atttttcact tctcttctgc ctaaatacag aagacgagat
     3601 ctatttaccg aaacaacgat tttggattta tttgaagaag gaatgagcca ttcagaaatt
     3661 tgttccatga tccaaaaatt atgtggagct cgctattcta aacagaccat atctaatatt
     3721 acggacaaag ctctggaatg tgtagattcc ttcaaaaaca gaatattgaa caaagagtat
     3781 gccgttgtat atcttgatgg aacatccatg cctttacgcc gtgatactgt atccagggaa
     3841 atggtccata tagcactagg aatcacagta gaaggtacaa aagaaattct tggctatatg
     3901 attgcaccaa acgagtccgc tgagatatgg gaagaacttc tgaaagacct taaatcaaga
     3961 ggggttgaac gtatttcttt attttgtaca gacggattgt ctgggatgga aaatgtaata
     4021 caggacattt tcccgggctc taaaattcaa agatgcctgg tccatattca aagaaatata
     4081 tgtgcaaaag taagagtcag tgacagaaaa gaagtggcca atgaatttaa agaggtatat
     4141 cggtccaaat caaaagaaga agctttaact tctctaaaga aattcatcga taggtggaaa
     4201 cacaaatatc catcaatggt gaaaagtctc agcaacaatg actatctgtt tacattctat
     4261 gattatccag aaagtgttag gcatacgatt tattcaacaa atctgattga aggaaacaat
     4321 aaacaattaa aacgaagttt caaaaagaaa gaacaatttc caacggaaaa atctgaagag
     4381 aaataccttg tgattcagtt caatcgttac aacgagaaaa acatgaatca cgtacataga
     4441 ggttttggac aaacaaccag agaagattgg ttcaagagct aatctgcttt accaaggata
     4501 tacttttaca cataattctt gacgctccct tttatttagt gccacttctt aataatccca
     4561 ttgttattat caaataaaaa atacaatata tggttttgaa taattttttg aaagaaggct
     4621 tcattttgag ttatctgata gtatgcttaa ttatcataat ttgtctgctc catctttttt
     4681 tatcgaaaac aatacctcgt tattccaaga ataaaaaagc tgaaaaattt tgtttattat
     4741 taaataaatt tactttaatt gctcctatcc ttgcatttat tattttttct gtacttttaa
     4801 gcactactct taaaggaaaa tttatggaaa gaagctctca tgcaatgatt ctcacttttt
     4861 tatggttgtt gttcaccaga atctatattt ttctgatgag cctgaaaccg cccaaatcca
     4921 ttagtttatg tttagttata aatggtattt ttttattatc acttatcatt tttattacac
     4981 ctttagatag atacgtcact tatttatata atcctttgga atactggact tattttatag
     5041 gtatattgga aggcattatt ttctacatcg ggtattttcc caataaaaat aacaactttt
     5101 atttttatcg caataaattg taataagtta ccatcctagc tactgagtat gtacatttac
     5161 tttttcatgc aaaaaagata aacgaaacag cttttctcca taataacaag ataattcata
     5221 catttatttg caattaaaga aaccacgtct acaggcgtgg tttctttttt agcgcctttt
     5281 cttttagacg catccgattt ttcccatctt gacatattga tagcaaagga catacctcgc
     5341 acttgggatt tcttgccgtg caatgatatc taccaaaaaa aatcagtgta tgatgtgtct
     5401 ttacccataa ttcttgtggt actttccgca tcagtgtttc ttctacttcc atgaccgtag
     5461 catctagttt gcagatcctc aggcgttttg agacacgttc cacgtgggta tctactgcga
     5521 ttgctggtat cccgaatgca tcccccaata cgacatttgc tgtctttctg cctacaccag
     5581 gaagagacat caattcttct cttgacgttg gcacttgccc atcgaaacgt tcaatcagct
     5641 gctgtgcaca cgctttgatg ttttttgctt tatttcgata caaaccaatc gtttttattt
     5701 taagaatgat ttcatcgatt gaagcttctg ctaacgcatc gggtgtcgga aaagaggcaa
     5761 acaaatctgg cgttgcttta ttcactgata catctgtcgc ctgggcactt aagatcactg
     5821 caatcaataa ctcaaatgga ttattgtgct tgagttctcc atgtgcttcg ggaaacattc
     5881 catacatcgt ttctagagct tccattgttt tttgtttgct taacatattc cgctactcct
     5941 ccaaccaatt tttcagtgag actttcggca attcttcttg ttgagctgac tgttctactt
     6001 ctctttgaag aagctgctgt tttcttcgtt tttggtcttc ttccacttgt tgttttgtac
     6061 gtagattttt tctttcccac gatagcaaaa tgcggtcaac gtaattaaaa ctataagcct
     6121 gatttaatac ggcttcccgc agtgctagct tcaaaatttc cggctgataa tggtcttctt
     6181 ctagccattg tccgatccgc tggaactcga tcgaagaaag cgggcgtcca aattcttgtt
     6241 caaataattg atacactgac tggatttctt tttcttgtgt tttttcttcg tatttttttt
     6301 cttgtgtttt taaatactca cctaatagat catagatggg gtaaagatta tatcgatctg
     6361 cttttttacc gttgacaatc gtcgtttcta tcttgataaa tccatttgtt acgagacgat
     6421 ccagtatttg aaaaatctga tcttgtttga ttcccatatc catagcaatc atctgcaaat
     6481 cagggaaagt atctccttca agctgtgcca tatgcaactg tagaacaaat aaaaattctt
     6541 cagattgtag accgatccta tgataatttt taagtaaaag attagatatg actgtttgtc
     6601 cagcgcttaa gtaattttct aaattcaaca tgatttctcc tccgctcaat cacagtatac
     6661 gtaaaaaaaa catgggaagc aagaacaggt ttcttttcta tttacttcac tgatcggtca
     6721 gatttacaat gtcgtctact tgatttttgt ttgttcatgt ttttgatgcg tatcttcact
     6781 tttcggaata aataaattaa gtagatttcg ctgttcctta gaatgttgac aaggaaacaa
     6841 aattactata cactattttt gtttccaagt taacattatt gcataaagga gtttttacat
     6901 gtacagtttc acaaataaac attctaaagt ttttggatac gtctctgtct tattcactgg
     6961 attaggattt actatcgtca gtcctgtact tccgttttta acgctgcctt attctcactc
     7021 catacatcag caagctttct atatcacttt gctcatgtct gtttatgctc tagctgcctt
     7081 cttatctgcc cccattctcg gtagcttaag tgatcatttt gggcgtcatc cgatccttat
     7141 tatcagttta ttaggatcaa gtatcggata tttgcttttt ggtttaggaa attcgatatg
     7201 gatgttgttc cttggtcgta tcattgaagg attgacggca ggagaaatct ccaccttata
     7261 tgcttacttt gccgacatca cggaacctaa tgaaagaacc aaagtttttg gatggatggg
     7321 tgctttagtc ggtataggaa caacgctggg tcctattata gggggtctat tagctgagtt
     7381 ggggaattct gttcctatat ttattggagc actctttact ttcttaaatg ccgtttatgg
     7441 ctacacattt atgctagaaa gccttccaat aaaaaaacgt tcagtagatt taagtttctc
     7501 ccatgtaaga ccttttcacc aattacaaca attattcaag atttcatccg ttattccttt
     7561 attgacagca ggttttgctg tttggttggc agctggctct cttcaatcta tcttttctca
     7621 attttcgatt gacgcctttc agtggaaggc tgggttggtt ggtttaagct tctctttgat
     7681 tggaatcttg gatactgtct cgcaattgtt cattatgcct cgattgctca aaaaattttc
     7741 agaacagcag atcacaagaa tagggatgtt cagtgaaatc ctcgcctatc tattcatcac
     7801 attatctgga attcttctac tgccgatttt gtttttattt gggattatct gttatggttt
     7861 cggtgattct atttttactc ctgtattcaa tggccaacta tccaattctg tctcagataa
     7921 ccagcaaggt cttgtaatgg gcggtaccca aagtatacaa tctctatcgc gcgtcatcgg
     7981 cccgctaatc gcaggacagc tttatgctgc agcaccattt cttccgacgg tattcggatt
     8041 tttgcttatg atttttgcct attatcacta caataaaaaa tagtagcttc atttgctact
     8101 tcgtataaat tctctgtatc gaccgataaa acgaaatact gtgtcaaatt cttcttttga
     8161 catattttca aatacagtag catctcgctg tacccatttt tgatggagtt gctcgtgtct
     8221 attccttatt ttttgtccga ttggcgtcaa ttgaaaatag atttcttttt tattctcttc
     8281 gctttgataa gtgtttatga tccctctagc tatcagtttt tttgttagct tactgatggc
     8341 tcctctagtc atgtaactag cattcgctaa ttttgtcacg ttaggatcag ggattttccc
     8401 gatatgttca atcacttcta gttcatttag agtaaatcct gcaaattctt tttccatttc
     8461 tggtttatta cgatgaataa tctcattagc taaatccaga tattcttcta ttagcagttg
     8521 tcttttatcc atttctacac cttctttttc tttcattata ttgttttttt gtttcctatt
     8581 caacaaagat aaaaatattt tgtaggaaaa cccaatctga aaccaag
//
LOCUS       DACDNO010000089         8432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDNO010000089 DACDNO010000000
VERSION     DACDNO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.089"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..785
                     /locus_tag="IWI22_002520"
     CDS             <1..785
                     /locus_tag="IWI22_002520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169851.1"
                     /translation="KDKIFKFLLLFTMPILLKTVADGYLSRNNIAVYLAFIGIYLVKE
                     KYIPKTVAKFSLLIIIPIGLFLFYIMSQIRLGTQSDISFWQGTWNLIDSESSYPQYYD
                     YANQGNSNVNIINFIIYIFIVCIPSSFYDLIHFSIPNLAYTFTETILGLSYGETNNYY
                     ILLPSVLGEAIMLCGKYFAFTYAFIYGIVSTWFLRLLKRNKSLEYLLIYYLLDFFRQF
                     RGGSQYVITAWETQLVPLIIIVFFYKLVIKNGVAKERNEYNV"
     gene            769..1734
                     /locus_tag="IWI22_002521"
     CDS             769..1734
                     /locus_tag="IWI22_002521"
                     /inference="COORDINATES: protein motif:HMM:NF021616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9169852.1"
                     /translation="MNTMCEVSIIIPSYKGSNEILIAVWGALSQTVTSKEIIVVDDNG
                     IGTEEQIKTKEKLQKFIKENKIKYFAHEKNLNGSAARNTGIKASNGKYIAFLDDDDYI
                     IPSKLEEQINVLEKTPKEVGMSVCEGYYVHKDGKGYLKKIKGNYVALDKYLLDKHYFN
                     TSSIVIKRECLIDMKGFDDSFIRHQDWEFCTRMLSRYKSIVVHKPLFIKYSFNRNNPS
                     NIEKRIENLDFFFEKMEDKLKDNLSLNIVNRIYKFKYGQIYQQYIINREFKAAKKFKN
                     QYHINIFTSLKDLVIHVIKRYSIALKKRTLSYEEMLVELERGMNY"
     gene            1734..2600
                     /locus_tag="IWI22_002522"
     CDS             1734..2600
                     /locus_tag="IWI22_002522"
                     /inference="COORDINATES: protein motif:HMM:NF025663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9169853.1"
                     /translation="MNIHNIGKKILCRTFPIIMTKYFYRKILGRKLKLKKPETFNEKI
                     NWYKLYFCPQNDLVIQCTDKFRVREYVEDMYCGDSLNDLIGVWDGVDEINWNSLPQRF
                     VLKCNHGCGMNIICPDKTSLDIEETKNRLNKWMKMDWGKESIEPHYSKIKPKIICEKF
                     IENTLDKESSLPPDIKIHCFNGIPKVIAYYSGRGNDMHGTFYDVNWNKLDFATKEGSV
                     ISKPICLDKMLLYAEKLSNPFPYVRVDFYECNNIPVFGELTFTPASGRSPYMTREADE
                     YMGNLLNLKEME"
     gene            2602..4107
                     /locus_tag="IWI22_002523"
     CDS             2602..4107
                     /locus_tag="IWI22_002523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase"
                     /protein_id="HAP9169854.1"
                     /translation="MRTKKAFYNTTTGIIYQLCSIVCGFIIPRLILGYFGSEYNGMIS
                     SITQFLGWISILTAGVGGVTRAALYKPLNKNDNYKISGIINATDNFMKKIALIFLGGL
                     IIFAIGYPFLLKNKFEWFFVFTMVLVLGINTFAQYFFGMTYQNLLEADQRQYIYSTVR
                     ILTTLISTLVTVVLIIMHFDIRIVKLASSLIFAVNPIFLNIYVKKYYSIDTKIAPDNS
                     AISQRWHAFAQEIAVMINNNTDIVVLTIFSNVLEISVYVIYNMVLSGVKQLLQTFTTG
                     IGAAFGSMIAEGIDNSSLNRKLKVFEVLVYNITTILFSVTLVTIVPFVRVYTSNINDV
                     NYIRPVFAIIITIAAMFNCFRIPYQTIVYAAGHFKQTRNGSIVEVILNLSISIILVIK
                     IGLIGVAIGTLVGSIFRTLQYGIYLSRNIIVRSNWLFYTKIVISLLETMIIYIISSIF
                     INISNVSYFGWISYAINIFMLSIAIVCMGSFLFYRKDTISIIKRLIFALKR"
     gene            complement(4405..4965)
                     /locus_tag="IWI22_002524"
                     /pseudo
     CDS             complement(4405..4965)
                     /locus_tag="IWI22_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177795.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5199..6491
                     /locus_tag="IWI22_002525"
     CDS             5199..6491
                     /locus_tag="IWI22_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9169855.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCLISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYIRRLWRPGFKAHLTETDIVDRLLKRSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(6779..8263)
                     /locus_tag="IWI22_002526"
     CDS             complement(6779..8263)
                     /locus_tag="IWI22_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP9169856.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRVESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDIPQNMMSAIVDSNRTRKDFIADRVLQKTGYYSYDDDN
                     TFDLSLEKEVIIGVYRLTMKSNSDNFRQSSIQGIMKRIKAKGATVIIYEPTLKDGSTF
                     FGSKVINDLEKFKSISHSIIANRYDSILNDVKEKVYTRDLFQRD"
BASE COUNT         2858 a         1182 c         1429 g         2963 t
ORIGIN      
        1 taaaagataa aatatttaaa tttcttctac tatttactat gccgattctt ttaaaaacag
       61 tggctgatgg atatttatca agaaataata ttgcggtata cttagcgttt attggtatat
      121 acttagttaa ggaaaaatat attccaaaaa cagtggcaaa attttcttta ttaattatta
      181 taccaatagg actattttta ttctatataa tgtcacaaat aagacttgga actcaaagtg
      241 atataagctt ttggcaagga acatggaatt taatagatag tgaaagttca tatcctcaat
      301 actacgatta tgcaaaccaa ggaaatagta atgtaaatat aattaatttt attatatata
      361 tatttattgt atgcatacca tcaagcttct atgatttgat tcatttttca attccaaatt
      421 tagcgtatac tttcacagaa actatattgg gtttgtctta tggggagaca aataattatt
      481 atattttatt accatctgta ttaggagaag ctataatgct ctgtggtaag tacttcgcat
      541 ttacctacgc gtttatctat ggtattgttt caacttggtt tttgagattg cttaaaagaa
      601 ataagtcttt agaatattta ctaatttatt atttattaga tttctttagg caatttcgtg
      661 gtgggagcca gtatgttata actgcatggg aaacccaatt ggttccttta ataataatag
      721 tattctttta taaattagtg ataaaaaatg gagtagcgaa agagaggaat gaatacaatg
      781 tgtgaagttt ctataataat tccaagctac aagggaagca acgaaatact aattgctgta
      841 tggggagcgt tgtcacaaac agtgacatcg aaggagataa ttgtcgtaga tgataatgga
      901 attggaacag aagaacagat aaaaacaaaa gaaaaattac aaaaatttat aaaagaaaat
      961 aaaataaagt attttgcgca tgaaaaaaat ttaaatggtt cagctgcaag gaatactggg
     1021 ataaaagcta gtaatggaaa gtatatagct tttttagatg acgatgatta tattattcca
     1081 tctaaattag aagagcaaat taatgtatta gaaaaaacac caaaagaagt tggtatgtca
     1141 gtatgtgaag gatattatgt tcataaagat ggcaaaggtt atttaaaaaa aataaaggga
     1201 aattatgtcg cattagataa atacttatta gataaacatt attttaatac gagttctatt
     1261 gttataaaaa gagagtgttt gattgacatg aaaggctttg atgattcttt tatacggcat
     1321 caagattggg aattttgtac gcgaatgcta tcaagatata aatctattgt ggttcataaa
     1381 cctttgttta taaaatatag ctttaatcga aataatccat ctaatataga gaaaagaata
     1441 gaaaacttag atttcttttt cgaaaaaatg gaagataaat taaaagataa tttatctcta
     1501 aatatagtta atcgtatata taaatttaag tatggacaga tatatcaaca atatattatt
     1561 aatagagagt ttaaagcagc aaaaaagttt aaaaatcaat atcatattaa tatattcaca
     1621 tcattaaaag acttagttat ccatgtaata aaaagatact ctattgcgtt gaaaaaaaga
     1681 acattgtctt atgaagaaat gttggtagaa ttagaaagag gaatgaatta ctaatgaata
     1741 ttcataacat aggaaaaaaa atactttgca gaacttttcc tattatcatg acaaagtatt
     1801 tttatagaaa aattcttgga agaaaactga agttaaaaaa acctgaaaca tttaatgaaa
     1861 aaataaattg gtacaagtta tacttttgtc cacaaaatga tttagtaatc caatgcactg
     1921 ataaatttag agtaagagag tatgtagaag atatgtattg cggtgattct ctaaatgatt
     1981 taattggagt atgggatgga gttgatgaaa ttaattggaa ctctctacca caacgttttg
     2041 tgttaaaatg taatcacgga tgcggaatga acatcatttg tcctgataaa actagtttag
     2101 atatagaaga aacaaagaat agattaaata agtggatgaa aatggattgg ggaaaggagt
     2161 ctatagaacc tcattattca aaaattaagc caaaaattat ttgtgagaaa tttattgaaa
     2221 atactcttga taaagaatct tctttaccgc cagatataaa aatacattgt tttaatggga
     2281 tacctaaagt tattgcgtac tactcaggta gaggtaatga tatgcatgga acattttatg
     2341 atgttaattg gaataaatta gattttgcta ctaaagaagg ttcagtcata agtaagccta
     2401 tttgtttaga taaaatgtta ttgtatgcag aaaaattatc taatcctttc ccctacgtta
     2461 gagtagattt ttatgaatgt aataatattc ccgtttttgg agagttaact tttacacctg
     2521 caagtggaag atctccgtat atgacgagag aggcagatga gtatatgggg aatttgctaa
     2581 atttaaagga aatggaataa aatgagaacg aagaaagctt tttataatac gacaactgga
     2641 atcatatatc agttatgttc tattgtatgt ggttttatta ttccaagact cattttagga
     2701 tattttggat ccgaatataa tggtatgatt tcatctataa cccaattttt aggatggatt
     2761 agtattttaa ctgcaggagt tggaggagtc actagagctg ctctttacaa accattgaat
     2821 aaaaatgata attataaaat aagcggaata attaacgcaa ctgataattt tatgaaaaaa
     2881 attgctctca tatttttggg gggacttata atttttgcta ttggttatcc atttctctta
     2941 aaaaacaaat ttgaatggtt ttttgttttc acaatggtat tggttttggg aattaatact
     3001 tttgctcaat atttttttgg aatgacatat caaaatttat tagaagctga tcaacgccaa
     3061 tatatttatt ctactgttag aatattaaca acactgatca gtactttagt tacggttgta
     3121 ttaattataa tgcattttga tatacgaatt gtaaaattag ctagttcatt aatttttgct
     3181 gttaatccta tttttttgaa tatttatgtt aagaaatatt acagcataga tacaaaaata
     3241 gcaccggata attcagcgat ttcgcaaagg tggcacgctt ttgcacaaga aattgcagtt
     3301 atgataaata acaatacgga tattgtcgtt cttactatat tctcaaatgt attagaaatt
     3361 tctgtatacg taatttataa catggtttta agtggagtta agcagttatt acagacattt
     3421 actacaggta taggggcagc atttggaagc atgattgcgg aaggaataga taatagtagc
     3481 ttaaatcgta agcttaaagt ttttgaagta ctcgtttata atataactac tattttattt
     3541 tctgtaacat tagttacaat tgtaccattt gttagagttt atacttcaaa cataaatgat
     3601 gtaaattata tacgtccggt attcgcaatt attattacaa ttgctgctat gtttaattgt
     3661 tttcgaattc cttatcaaac gattgtatat gcggcaggac atttcaaaca aacaagaaat
     3721 ggttctatag ttgaagttat tctaaatctt tcgatttcta taattctagt tattaaaata
     3781 ggactaatag gcgttgcaat aggtacatta gtgggttcta tatttagaac gctacaatat
     3841 ggtatatatt tatcgagaaa tataatagtg cgctctaatt ggttatttta tacaaaaatt
     3901 gttatttctc ttctagagac aatgattatt tatataataa gctcaatatt tataaatata
     3961 agtaatgttt cgtattttgg ttggattagt tatgcgataa atatatttat gttatctatt
     4021 gctattgtat gcatgggtag tttcctattt tataggaaag atactatttc aatcataaag
     4081 agattaatat ttgctttgaa aagatgatat aaaaagtatt tttaatttga gaatatctat
     4141 tgcgactatt agactattaa tttggttgag gattagatag tcgtgaaatg tttattctat
     4201 tacattagat tttttagtaa taactatata aattatctaa attaagattt aaatgatagc
     4261 ttatagaaaa taaccttatt ttagataata tttctattaa ttatgtttaa ttgattgcat
     4321 tttgtagaga gtgggacaat agtcctttct ccaaaagaaa acaagtccga ttttgatggt
     4381 taatttcaaa atcggacttg ttttttcttc ttttaaataa ggcaagattt cttctttata
     4441 aagggcataa tagaattcgt ttgattgctt atccaaagca gtttcataac gattcgtttc
     4501 tttacgccag aagaaactga acttgttggc atctgcttca atttttgtac catcaataaa
     4561 tattgcttca ttatcaatta cttaattggt gatcgattgg cggctaaata agacaaacgc
     4621 ttcagctaaa aggtcagcgg gtgtttcctg acttcggaat cgattgatgg tccgatagct
     4681 gacttgttcg tggttagcca accaacacat acgatagctg tcatctaata gaaattcaat
     4741 tttccgacca gaaaagacac gctgcatata agcatataga ataattttta acatcatttt
     4801 ggggtggtag gccggtcgtc ctagttattc ttataattca agaaagagtg tttgtggcat
     4861 tgctacgact aaagcgttga ctgcaaaagc gacatctgtt tcaggaatat attgttccat
     4921 atctaatggt aaagttgttt gattgatggg ataattttta aacataaagt actcgcttcc
     4981 tttctgattg ggcttggtca ctttaatctt acagggatga gtgctttttg tgtaccttaa
     5041 aatcaaaaaa agtggggcaa taatcagatt ttggctctat actttttgat gttggtggat
     5101 gacaccagca tcttttttat tttccaaaac atatagaaaa gacaccttga aatcctttag
     5161 aattaatttg gcgaaccaac ccaaaaggag gaatcaagat gtcttacact caccttatca
     5221 aagaaacatt agatattcta gacttatcag ttacttttaa cgaaaattgt ctcacaaaag
     5281 aaaaatacaa agggcaaatc tgtcatattt atcgtggaaa cttgatttat acagcccaag
     5341 aatgcatcca ctgtaaacac cagattgctt cagatattgt gcgttggggt acaacaaccg
     5401 tccggttgtt aatgaacgat gtttctgaat accgtaccta cctcgaattg aaaaagcaac
     5461 gcttcaaatg caaagcttgt cagcggacct ttgtcgcaga tacttctgtt gcgaaaaaac
     5521 attgtctcat cagtgagaaa gtccgctggt ctgtcgtgac tcgtctgaaa aaaaatacct
     5581 caatgacaga gatcgccgct cagaaaaatc tatcggtctc ttctgtttac cgtattatga
     5641 agcgatttta tcgtccgttg aatccttttc ggacaccgtt acctaaggta ctttgttttg
     5701 atgaattcaa atcagttcgg ggtgtttcag gtgctatgag tttcatcatg atggatggac
     5761 aaacacagcg cttattggac attgtcgaga accgacaact gcctttttta aaacgttatt
     5821 ttagtcattt ttcacgagaa attcgtgaag cggtcgaatg gatcgtgatt gatatgtacg
     5881 ctccttatgt atcgctcgtt aaaaagctgt ttccaaaagc acagctgatt attgaccggt
     5941 ttcacatcgt ccaacatatt ggtcgtacct ttcgcaatca tcgaatcaaa gaaacgaacc
     6001 aactattaaa aagcaaagaa caaaaacact atcaattagg taagcaatta aaaagatact
     6061 ggaaacttct tcaaaaagat gaacgcaagc ttgattatat tcgacgcctt tggcgacctg
     6121 gcttcaaagc tcatttaaca gagaccgata ttgtggatcg cttactgaaa aggagccctg
     6181 ctttgcgtgt aggctatcag ttatatcaag actttcttta cgctgtaaag gaacgagact
     6241 acgtatcgtt tgaagaacta cttaccaata atatcatgct tcccgaaggc tatcagacga
     6301 ccttgagaac gttccagaaa tttctgccac aaatcaaaaa tgctttacaa caaagttatt
     6361 caaatggccc acttgaatgt ctgaacaacc atatcaaagt gttaaaacga aatgcatatg
     6421 gctttcgtag cttttataat ttcaaattgc gcatcatgat tcgtcatggc aacgcattga
     6481 ttttcaacta aaaaagatca agaggaactg atccacttga tctccaatta ttctaagatt
     6541 tctctttacc aacatcattt gacaaagagc ccagattttt ttctgactat tgtcccactc
     6601 cccttgcgtt gtaaaaattt tgataattag atttcttaaa gttggtaatc ttttattaaa
     6661 aatcttttct aaagagagac gtaattcctc ctagcgctta attaatgggt ctaaatgaaa
     6721 ggataaaaat tagtcttgct gtttattgta aattatttta atgtatctaa tcattcgttt
     6781 aatcccgttg aaataaatct ctagtgtata ctttttcttt tacatcatta agaatactat
     6841 catatcgatt agcaataata ctatgactta tgcttttaaa tttttctaaa tcatttatta
     6901 cttttgaacc aaaaaatgta ctaccatctt taagtgttgg ctcgtaaatt atcacagtgg
     6961 ctccctttgc cttgattctt ttcataatac cttgaataga agattgacgg aagttatccg
     7021 aattactttt cattgtaagc ctatagactc caataatcac ttctttttct agagacaaat
     7081 cgaaagtatt gtcatcatcg taagaataat atccagtttt ttgaagcacg cggtctgcta
     7141 taaagtcttt tcttgttcgg ttactatcga caattgcact catcatattt tgaggtatat
     7201 cgttatagtt agttagaagt tgttttgtat ctttcggtaa acaatagcct ccatatccaa
     7261 aagatggatt attataatgt gtgccaattc ttggatctaa gcaaaccccg tgaatgattt
     7321 cttgtgtatt tagtcctttc atttctgcat aagtatccag ctcattgaag taagatactc
     7381 gaagtgcaag gtaagtatta gcgaataatt taactgcttc agcttctgtg aatcccatga
     7441 aaagagtttc gatatctttt ttgattgctc cttcttggag taaatctgca aaagtgtgag
     7501 aggcttctcg taaagttttg tcagtttggt ccgtgccgac aataattcgc gaaggatata
     7561 gattgtcata taatgcctta gattctctga gaaattctgg gctgaataga atatttttag
     7621 tattgtattt tttacgaata ctttcggtat agcctacggg taccgtcgac ttgatgacca
     7681 ttattgcctt tggattgact tctaacacca atttgattac tgactctaca gcagaagtat
     7741 caaaataatt ttgtttacta tcataatttg ttggagcagc aatgacaaca aaatcggctt
     7801 ctctataggc ggctgcacca tcagtcgttg caattaaatt aagatttttt tcagctagat
     7861 atttttcaat gtaatcgtct tgtattggag atttcttatt gttaatttgt tttacttttt
     7921 caggaagaat gtctactgct gttacctgat tgtgctgggc aagaagagta gcaatggaaa
     7981 gacctacgta accagtaccg gctacagcaa tattcttcga ctgatgtttt gttctgtttg
     8041 atttatttga ttcatttata aatagatctg ttggttcaaa atttagaact tctgctaatg
     8101 attctaattg ttgtattgac gggataaaat cgagagattc tactcgacta attaaggcac
     8161 ggttgatacc agtagctact gcaagttgtt gttgtgtcat tttcagagat tttctagaat
     8221 caataatgat ttgagctaaa cgtttttgag ataacttttt catgatgcac tccttttttt
     8281 gttgctaata ataacaaaca attttttctt ctataacaga atagcttatt attaataaaa
     8341 ctgtcaattt ttttattgat taaaaagata ttttttgatt ttaagtattt tcaattattt
     8401 tttgttactt aaaataactt tatagattgt ga
//
LOCUS       DACDNO010000090         8251 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDNO010000090 DACDNO010000000
VERSION     DACDNO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.090"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IWI22_002527"
     CDS             <1..197
                     /locus_tag="IWI22_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169857.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IWI22_002528"
     CDS             314..811
                     /locus_tag="IWI22_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9169858.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IWI22_002529"
     CDS             898..1290
                     /locus_tag="IWI22_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9169859.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="IWI22_002530"
     CDS             1274..3721
                     /locus_tag="IWI22_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9169860.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="IWI22_002531"
     CDS             3724..5907
                     /locus_tag="IWI22_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9169861.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="IWI22_002532"
     CDS             5904..6905
                     /locus_tag="IWI22_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9169862.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="IWI22_002533"
     CDS             6920..7834
                     /locus_tag="IWI22_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9169863.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="IWI22_002534"
     CDS             8079..8195
                     /locus_tag="IWI22_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9169864.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DACDNO010000091         8228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDNO010000091 DACDNO010000000
VERSION     DACDNO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.091"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IWI22_002535"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IWI22_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9169865.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IWI22_002536"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IWI22_002536"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9169866.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IWI22_002537"
     CDS             2591..3376
                     /locus_tag="IWI22_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9169867.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IWI22_002538"
     CDS             3389..4240
                     /locus_tag="IWI22_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9169868.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IWI22_002539"
     CDS             complement(4364..4816)
                     /locus_tag="IWI22_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9169869.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IWI22_002540"
     CDS             complement(5281..5649)
                     /locus_tag="IWI22_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9169870.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IWI22_002541"
     CDS             5799..6281
                     /locus_tag="IWI22_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9169871.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IWI22_002542"
     CDS             6318..7328
                     /locus_tag="IWI22_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9169872.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IWI22_002543"
     CDS             complement(7443..8156)
                     /locus_tag="IWI22_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169873.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACDNO010000092         8067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDNO010000092 DACDNO010000000
VERSION     DACDNO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.092"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..883
                     /locus_tag="IWI22_002544"
     CDS             <1..883
                     /locus_tag="IWI22_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9169874.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="IWI22_002545"
     CDS             931..3060
                     /locus_tag="IWI22_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9169875.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIESVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="IWI22_002546"
     CDS             3035..3724
                     /locus_tag="IWI22_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169876.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4818)
                     /locus_tag="IWI22_002547"
     CDS             complement(4132..4818)
                     /locus_tag="IWI22_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9169877.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4954..5148)
                     /locus_tag="IWI22_002548"
     CDS             complement(4954..5148)
                     /locus_tag="IWI22_002548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169878.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="IWI22_002549"
     CDS             complement(5198..5638)
                     /locus_tag="IWI22_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9169879.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="IWI22_002550"
     CDS             complement(5642..6487)
                     /locus_tag="IWI22_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9169880.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="IWI22_002551"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="IWI22_002551"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9169881.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2481 a         1514 c         1713 g         2359 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa tcggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccgggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaaa
//
LOCUS       DACDNO010000093         7926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDNO010000093 DACDNO010000000
VERSION     DACDNO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.093"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(144..524)
                     /gene="yajC"
                     /locus_tag="IWI22_002552"
     CDS             complement(144..524)
                     /gene="yajC"
                     /locus_tag="IWI22_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9169882.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(621..1766)
                     /gene="tgt"
                     /locus_tag="IWI22_002553"
     CDS             complement(621..1766)
                     /gene="tgt"
                     /locus_tag="IWI22_002553"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9169883.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1984..2682
                     /locus_tag="IWI22_002554"
     CDS             1984..2682
                     /locus_tag="IWI22_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9169884.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2850..5489)
                     /locus_tag="IWI22_002555"
     CDS             complement(2850..5489)
                     /locus_tag="IWI22_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9169885.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6108..7139)
                     /gene="queA"
                     /locus_tag="IWI22_002556"
     CDS             complement(6108..7139)
                     /gene="queA"
                     /locus_tag="IWI22_002556"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9169886.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7332..>7926
                     /locus_tag="IWI22_002557"
     CDS             7332..>7926
                     /locus_tag="IWI22_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9169887.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDE"
BASE COUNT         2242 a         1716 c         1457 g         2511 t
ORIGIN      
        1 agttatttat tattatttat atgacacacc cgattattca atattgagca atatatccca
       61 tgctcttatc tctttctttt ttgaataaaa aatgaggtag gacatctttc tgccctacct
      121 cgtctttaaa taagctttga tacttatttt tcttccttct cttctgtctc ttccagcgtt
      181 tctgttgttt cagtttttac ttcaactgtc tcagctggtt cttctgttgt ttcaacagta
      241 gtcgttgcat catttgttac gacagtccct ggtttgaccg ttttgattgc tgcacgatcg
      301 tactcaagga agatcccttc gcagtctaaa acaactgtcc gtttgtcatt gtcgatttct
      361 gaaacaactc catgtaatcc gccgatcgtt acgactgaat cacctggttt catgctatct
      421 aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa aaaccacatt
      481 gctaatagcg cgataaacat cacaagcatt ggtaatgttc ccattttttt cacctcacta
      541 attctgtatc tacaaaaaac actttagcag aaaaatgctg ataacactag ttttttactt
      601 aatttttaaa agattttcct ttagaagctt tttgcgtttt ctttattgaa cccgtattct
      661 tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg acgtacttgt
      721 ttcattagat tcaacaagaa gtataaattg tggtaagaag tcaaacgaat cccaaatgtt
      781 tcatcacatt tgatcaaatg gcggatatag gcacgtgtat agtttttaca tgtataacaa
      841 tcacatttct catcaattgg acggaaatca tgggcatatt tcgcattttt cactactaga
      901 cggccttgag aagtcataca tgtgccattt cgagcgattc gagtaggcaa gacacagtca
      961 aacatgtcta ctccacggat aacaccatcg atcaatgaat cagcagcacc gacacccatc
     1021 aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga attctaatac acgattcatt
     1081 tctgatttag gttccccgac agataacccg ccgattgagt aaccagggaa atccatcccg
     1141 actagatctt tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg gatgattcca
     1201 aataatcctt gacggtctgg attagcatga gctttcaatc ctcgttctgc ccatcgagac
     1261 gtacgttcta ctgatttttt cacgtagtcg taactttcat cgaatggtgg acattcgtca
     1321 aaactcatca tgatatcaga gcccaattta ttttggatgt tgattgcttt ttctggtgat
     1381 aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc ttcgatattc
     1441 cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat cggttgatcc
     1501 cagttcataa atttatgcag tccgccagct tcttctacta agtcttcacc agggcgtagc
     1561 cataaatgat atgtattact taaaataacg ccagcaccca tttctttcaa ctcttctggg
     1621 gacattgttt ttactgtagc taatgttcct actggcatga acattggtgt ggggaaagtt
     1681 ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat gtttttcttt cttgattaaa
     1741 cgatagcgaa tggcaggttg cgtcattcat ttccctactt tctatataaa atatcttgac
     1801 aactcccacc atcataatcg tttcaagtgc tttttgcaag ttccctcggc ttccttctac
     1861 tttatttaat attctttttt ttatcctttt tttatgaata acaaaaaaaa gaaaaacatt
     1921 atgacagaaa tattttttga tacatggtat attggtgtta ttaaagaaaa ggagggaaaa
     1981 atcatgcaat cttctgcaga attaaaacgc caagcaaaag agagtctaaa aggacgttgg
     2041 ggacaagctg ttttactaaa tctaatccct acattgattg gtattgctac tattcttctt
     2101 attatgattc ctattatctt gtttgccacc accatatatg atggatcagc gagcgtctca
     2161 gaagcgacaa cttcaggagg cggtagttca ggaggcggtg gcggaattat ttccagcatc
     2221 attagcgctt tgttcatgag cggtatttct tggacttatc ttgatatttt acgtggacaa
     2281 aaagatcaga tcgaaccatt caaagatgca tttcgtggat tcaaaggttt gtttatcggc
     2341 ggagtgatca gcttagctgt gctgatctcg atctttacaa cattatgggg tttgctatta
     2401 gttattcctg gaatcattaa aagctattct tattctcaaa gctattttat ttactacgat
     2461 atcgtccaag aaacaggaca aacacctaaa gtactggata cgatcactgc aagtcgaaaa
     2521 ttgatggatg gttataaagg acaacttttc tggttagatc tttcatttat cggctggcat
     2581 atcttagcat tagcaactgc tggtattggt tatctatggc tcagcccata catttctgca
     2641 acaaaagcag cattttatga acaattgcca aaagatattt aaaagagagt gtgacttgtg
     2701 accgaggggg tggcaggaga acaccgttta tcgagaggca gtaaaagaaa cattcagccc
     2761 attagcaaaa aggaggtgtg acaacattca tgtcacacct cctttttgta tagttccagt
     2821 aaaaagaaaa tcaacttaga aacagttaat cattcctctt cttctgcttc cggtaatcga
     2881 tggaacttga tcagttcatt caccgcgatt ggtacgatag ataaaatccc taccaagaac
     2941 cagtgattca aactgatgtg tactaggccg aatagttctt gcgtaaatgg cagcaaagca
     3001 acagccgttg taatagctaa tgccagtaac gccatttcta ccaaagcttt attggtcgct
     3061 aaattgactt tgaagatcga ttggctgctg cgtacattga acacatgcag aatcgatgaa
     3121 taagctaaga tcaagaatgc aacagtttga ccgacatgct gacttgcgtc tacccatggg
     3181 ctgatagtat cgacataact acctaaataa taaccaaaca aggttacgat ggtgaagacc
     3241 cctgcattta tggcaatttt ttgcaataaa cctcgagcaa agatcccttc atttttcgga
     3301 atgggtggct gatccatgat gccgtgttcc gctggctctc ttcctaaagc aaatcctgga
     3361 agtccatcag caactacatt gacaaacagt agttgcacag ctgtaaatgg tgctccccat
     3421 cctaacagca tcgcaatcaa aacaataaag atctccgaaa tattacagct tagcaaaaag
     3481 ttgatagctt tacgaatatt ctgataaacc gaacgtcctt gactgacagc gtctacgatc
     3541 gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac tttgcgccac atctgttcct
     3601 gtgatcccca tggcacaccc aacgttactt gctttcaacg ctggtgcatc attcacacca
     3661 tccccagtca tcgctacgac tgcacccgta cgttgccaag atttgacgat ccggatcttg
     3721 tcttctggcg ttacccgagc atagacagag agagctttga cacgcgcatc taattcttcg
     3781 tctgataatt gatgcagttc tgatcctgaa agtgcttctg acggatcatt caagatacct
     3841 aattctttcg ctatggcact ggcagtcacc acatgatctc cagtgatcat gactgttttg
     3901 atgccagcct ttttcgctcg cttgattgct cctttacttt ctggacgtgg tggatcgatc
     3961 attccgatca gaccaatcaa acgtaagtct ttttccagtg cttcagaaga aatatcttgc
     4021 ggttcttcgt cataagtagc ataaccaaca gcaatcactc tcagtgcccg tttaccaaag
     4081 cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac taaagcgcgg agcaagaaca
     4141 tcgaatgctc ctttggtgat tgagatgtat ttttcgccca tttggtgaac tgttgtcatc
     4201 atttttcgtt cagaatcaaa aggaagctcg tttactcgtg gatatttttc ttctaattct
     4261 gcctttgtat gataattctc ttcaacagcc cttacgatgg ctacttctgt tggattacct
     4321 ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat ctgtacatag tgcagccatt
     4381 ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga tattcgttac ttcatcttct
     4441 ttgctccata cccggcggac gcgcatttca ttttgtgtca gcgttcctgt tttgtctgag
     4501 cagataacat tagcggttcc tagcgtttca actgctggca actgtcggat gatcgcatgt
     4561 ttcctcgcca tcttctgcac accgtatgca agtgtcaacg taacaataac agtcaaggtt
     4621 tccggtacag ctgcaacagc caaggaaatc gaagtcatga acatctcaag gagtggttct
     4681 ccttgcagtt caccaatcac aaatactaaa gccgcagccg ctaaagcgat catactgatt
     4741 cgtttgccta gctgattcaa tcggacctgt aaaggtgttt tttgcatagt attttcgttc
     4801 aaaagatccg cgatcttacc catttctgta gccatgccaa tggcagtgac gatcgctttc
     4861 ccacgacctg cagcaaccgt acatccttta aataccatat taagttggtc acctaatgac
     4921 tcatcttctt cagcaataaa tgctgcatct ttttcaactg cttcactttc tccagtcaac
     4981 gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac gggcatcggc agaaatagca
     5041 tcaccggact ctaaaacaag aatatcaccc actaccagtt gctctgactc aacagtggtc
     5101 actttgccgt ctcgtatgac agttgccgtt tgtttattca tatcttgcaa ggattccagt
     5161 gctttttctg cgtttccttc ttggacaatc gccaataccg agttgatcac aacgatagcg
     5221 atgatcagca gtccttcaaa taaatcccca tgttctgtag caaatgcggt ataaaaggaa
     5281 atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg acaagcttcg aaagaatttt
     5341 ttgatcatcg attctttcgg cgcctcatcg aatttattca atccctgctt ttttcgccgt
     5401 tcatccactt cttgtgaaga caacccagta tctggatctg cttctgttac cttctttact
     5461 tcttctattg tctgcttgta ttcttccatc ctattcctcc tttaaaatct tcgtacaaaa
     5521 aaaggtactt attttactat aacatccttt ttttatttac ttcaaataaa gggcttatcg
     5581 attatttgtt aagaaagcgt aaaatttaaa gtgcttatat ttgcaaacgc taccaaagga
     5641 tgctattata tagatgtaag gaaggaggat accatcaaat agacagaaga atttctcgat
     5701 agaacagtgt aagcaaacaa agttgttaga agtacaggtt tcacccagac atgcgtgtag
     5761 agtacaaacc tataaatagt gaatgcgtat ggatgtttgg atcacgaagt atccgaaaca
     5821 agaagtaatg cgatacgtaa caattgaata acgtaaacgt ctgaaaattg aataggaaca
     5881 agagatgttt caaatgttga agatgtaacg aaaacaaaat ttttgtttaa catgaagaaa
     5941 ttcaacaccg tgctttgcat gtatgatgga aagttacaat catttctgtt tatttgaatc
     6001 cttccgaggt acaagaaagc cgagcttatc gagagctcgg ctttttcttt gtcataaaat
     6061 ctttgtaaag cttttttgtt attcaaaact agcggctttt cataacctta tttcacgaac
     6121 atcgcatctc caaaactaaa gaaacgatag cgctcttcaa tcgcatgctg gtaagcagat
     6181 aaagtcaatt ctcttcctgc aaaggcacta actaacatga ctaaagtcga ttttggcaaa
     6241 tggaaattag ttgagaaagc ttctaccact ttgaattcat agccaggcgt aatgaaaata
     6301 tcagtccatc cgctgtctgc tttgatttct ccattaaatt tcgttccaat cgtttctaat
     6361 gttcgaattg aagttgttcc gacagccacg attttcccgc catttttacg tacttggttc
     6421 aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac tatgcatatg gtgttcttca
     6481 attttatcta cgctgacagg tcggaatgta cctaacccta catgtagtgt cagatagact
     6541 aattcaacac cctttgcttc gatctcatca agtaattctt tcgtaaaatg caatccagct
     6601 gttggcgctg cagcagaacc attttctttc gcatagacag tttgatagcg atctgggtct
     6661 tccagacgtt ctttgatata tggaggcaat ggcatttctc ccaatgattc aagaatctct
     6721 agaaaaattc cttcatactc aaagtcaatg atccgtccgc cgtgttccaa ttcttctttc
     6781 actactgctt gcaatctgcc atcaccaaaa gagattcttg tcccaacttt ggcacgtttc
     6841 gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat tcgttagaag aaggacttct
     6901 aaatgcgcac cagtatctgg tttttctcca tataaacgag caggcagaac tcgtgtatta
     6961 ttcataacta gcgcatcacc aggctgtaac tcttcaatga tttcatggaa atgtttatct
     7021 tccatctctc cagttttccg atccaaaaca agtaaacgag aatgatcgcg gtcttttaat
     7081 ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa aatcttctgt tgttaacata
     7141 tcgtcactca ctttctatca tgtgcttcat cattctatca tgtttttcgt tctgttggca
     7201 aaatcagcac tttttcactt ctattttttg ttttcctaaa taaaaaaact accctatttg
     7261 tattttttca ttgttttagg catactagta atagatagtt cttcattgaa aatagaaaaa
     7321 gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat ttataaaggt ggaaaagtcg
     7381 ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt catttgtttt atcggtacga
     7441 gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg aatgactgat ccaacacaag
     7501 ggaaaatttt gattgatgga aaatccatcc aagaaattga tccagtggaa ttgcgacgtc
     7561 agatcggcta tgttatccaa agtattggat tgatgcctca catgacgatc cgtgaaaata
     7621 tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga acggaataaa acagctgaaa
     7681 aaatgattga tcttgtggaa ttgccccgtg agatgttaga tcgctaccca catgaattat
     7741 ctggtggtca gcagcagcgt attggagtcg ttcgtgccct tgcagcgaat caagatatca
     7801 tcttgatgga cgagccattc ggtgcgcttg atccaatcac tcgtgattcc ttgcaagacc
     7861 ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt atttgtaacc catgacatgg
     7921 atgaag
//
LOCUS       DACDNO010000094         7673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDNO010000094 DACDNO010000000
VERSION     DACDNO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.094"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            98..475
                     /locus_tag="IWI22_002558"
     CDS             98..475
                     /locus_tag="IWI22_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9169888.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            463..801
                     /locus_tag="IWI22_002559"
     CDS             463..801
                     /locus_tag="IWI22_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9169889.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            935..1378
                     /locus_tag="IWI22_002560"
     CDS             935..1378
                     /locus_tag="IWI22_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9169890.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            1640..2455
                     /locus_tag="IWI22_002561"
     CDS             1640..2455
                     /locus_tag="IWI22_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9169891.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            2465..3127
                     /locus_tag="IWI22_002562"
     CDS             2465..3127
                     /locus_tag="IWI22_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9169892.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            3332..4996
                     /gene="alsS"
                     /locus_tag="IWI22_002563"
     CDS             3332..4996
                     /gene="alsS"
                     /locus_tag="IWI22_002563"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9169893.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            5009..5719
                     /gene="budA"
                     /locus_tag="IWI22_002564"
     CDS             5009..5719
                     /gene="budA"
                     /locus_tag="IWI22_002564"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9169894.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            5849..6346
                     /locus_tag="IWI22_002565"
     CDS             5849..6346
                     /locus_tag="IWI22_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9169895.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            6531..6791
                     /locus_tag="IWI22_002566"
     CDS             6531..6791
                     /locus_tag="IWI22_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169896.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(7153..7392)
                     /locus_tag="IWI22_002567"
     CDS             complement(7153..7392)
                     /locus_tag="IWI22_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169897.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         2549 a         1175 c         1594 g         2355 t
ORIGIN      
        1 taatagaaat gagtattgac ttggagtaca cttaaagttg tagaataaat aaaatacata
       61 agtaaagtgg caagaaatta agagaaagag tgatcagatg aatatcaaac aagtcagtga
      121 agaaaagggc atttcggccg atacattgcg ctattatgaa cgaatcgggt tgattccacc
      181 agttaatcgg acaaaaggcg gaatccgtga ttatacagaa gaagacctaa aatgggtcga
      241 ctttacgatc tgtatgcgaa atgccggatt atcgatcgag tctttgattg aatatattcg
      301 cctatctagc gaaggagaag caactgtatt tgagcggcgt aagcttttga ttgaagaaag
      361 tgaacttttg acgaaaaaaa ttgctgaaat gcaagaatgt ttggaacgtt tacaaggaaa
      421 aatcaagaaa tatgatcgtg tgttatctag aaataaaatg gaatgtatca agtaaaaaaa
      481 tcaaggagca tcgctatgga gttacgctta ctaaaatatt tttggacaat tgcagaagaa
      541 ggaacaatct ctcaagccgc agaagttcta catcttacac aaccaacctt aagccgacaa
      601 ctgaaggagt tagaagagga actaggaaca gaactgtttg tacgcgaaaa ccgcaaaatg
      661 atcttgacag aagcaggtta ttttctaaaa aagccgagca gaagaaattt tagatttaac
      721 gaatcaaacc actcaagaat tcgagaacag aaaaaaacaa ttattcagcg gccgatttac
      781 gatcggctgt gtggaagctg ataattcgaa tacattggca ctattactag aaaagttgat
      841 ccgtgattat cccagcgttc aatttcatat ccacagcgga acaagtgatg atatcacgga
      901 gtgactagaa aaaagcctgt tagatttagc gattttgcta gatccagtag atacagagaa
      961 atacgaaacg attttgttac caagaaagga aaaatgggga ttgctcattt tagaaaattc
     1021 tgttttagca aaaaaagacg tgataacaaa agaagacttt gcgaatgtac ctcttttgat
     1081 ctcaaagcgt ccagaaatac agcgaatgtt tgctgactgg ttaggcataa aaaaggagca
     1141 gttgctgatc acaggaacgt acaatttgat tttcaatgta tttgcgctaa ttgagaatca
     1201 ggtaggtgct gctttagtca tagaaggtgc tgtaagtcat ctttcaaagc gagtgacatt
     1261 cgttccgctt gacccaatga ttgaaacgaa ctgcatgcta gtctggaaaa agaatcggat
     1321 tttatcacct gtagtcaatg aattgattca ccgtttcaaa gaagcttttg aacagtaata
     1381 agtatctcta atcgaagaaa tttcaataca aaagcgagac gtatgacagt cttgcttttg
     1441 tattttttca tttttaaatc aaaaaaaata aagagattta cataacttgt taaataagcg
     1501 cttacaaaca taagaagaac ggaaatgaat cagctataat aaccgttgtt ctggattgta
     1561 atcattacaa agaaatgaag attataactt tggaggtgtt cgaaatgaaa gaaaaaaagc
     1621 aaaaattcgt tcaaaaaaca tgccctttat tcaatggttg ggtatgattc tgctgattgt
     1681 attagcttta ttgacgatca ttcctttttt atggatggtt tctgcttcat tcaaagcaaa
     1741 taatgaagtg ttcacgattc cgattcaatg gatccctaaa acttggcatt tagagaatta
     1801 tctggttatt tggtctcgta ttcctttagt gacatttttt aagaatacag cttttttaag
     1861 cgttgtgatc acgttgattc agttgtttac ttctagtttt gctgcttacg ggttttctaa
     1921 actaaatttt aaagggcgcg atattttatt tttagcatat atcggcacga ttgctgtccc
     1981 ttggcagtct tatatgatcc cgcagttcat tatgatgaga aaagtgggtt taacatatac
     2041 gttgtggtcg ttggtgctat tacaagcgtt caatgctttt ggtgtgttct tgttgaagca
     2101 atactacagt agtatcccag attctttatg tgagagtgcg cggatcgatg gcttatcaga
     2161 gtggggaatc tatcgcaaga tcattttacc tcttacaaaa ccggcgattg ccagtttgac
     2221 gatcatcaca ttcgttaata catggaacga ttatatgggg ccattcattt atttgtcttc
     2281 tacggaaaat aaaacaatcc agttagggtt gaaaatgttt attgggctat ttgatgcaga
     2341 gtacgcatta atcatggctg cctcagttgt ttctgttctt cctgttgctt ttatattttt
     2401 agcgatgcaa aaatattttg tagaagggat cgcttcatca ggaatgaagg aataggaggt
     2461 gattatgaaa gagcagcgtt ttgacaaaaa tgtttatatg gaattctttt attggttgta
     2521 tcaatcattg gcaagtagcc tttgtttttg gtgtgtcaat atcttgtata tagcagctgc
     2581 gttccttttg tcgatagatg gaaggaatgt cttttccttt attttttctt tactttttat
     2641 tggtccaggt tgcatcgtga tgataggtat gacagaagaa ttagcaaaag aaaaaagttt
     2701 ttcaccagta cgtttgttca taaagacgtt tcgccaattt tggctaaagg gattttttta
     2761 ttggctattt gcttggatag ttagtgtgat aatgattttt gattgtttct tttttatacg
     2821 tttttcttat ggaaaatggt tgattccgtt gtttgtttta cttgcttgtt tatcagtttc
     2881 ctttagcatc aactactggt attttcaggt gagaaatcca gcgtcgaaac caaatcaagt
     2941 tctacggata gcgttctact atacgttgaa aaaatggtat gtgagtttac tagatttttt
     3001 actattgaca agtttgtttc tcttcttttt cgtaaagccc caatggtgta ttctactagg
     3061 tccttctatc gtatttggtt tgatttactt caacaatcgt aaattgatga gaactatgga
     3121 tctgtagact ttataaattg aaactttcaa ttaagaatga cagtttttat gtaaaaactg
     3181 tcattctttt tcattttttg aaacatatgg ctaaacaaat gaaataaaac gtgctatact
     3241 tttgaatgaa acaggtttag aaactgtatc aattatttgt gattatttct aactaataat
     3301 taaaaataaa gcataaaaag gacgatgttc aatggacgaa aaaatgaaac aaggttcgaa
     3361 atctgtagta gaaagtttga ttaatcatca tgttgattat gtattcggta taccaggtgc
     3421 aaaaatcgat ggtgtattta atgaattaga ggatcaaggt cctgaattaa tcgtgactcg
     3481 tcacgaacaa aatgctgcat ttatggcaca agcaatcgga cgtattacag gagagccagg
     3541 agtagttatc gcaacaagtg gtcctggtgc tagtaactta gctacaggtt tggtaacagc
     3601 gacagcagaa ggtgacccag ttttagcaat tgccggacaa gtaaaacgaa gcgatctatt
     3661 gaagttgacc catcaaagca tggataatgc agcactgttc cagccaatca caaaatacag
     3721 tgcagaaatc caagatccag aaacaatttc agaagtgatt gccaatgctt atcgtatggc
     3781 taagagttca aaaaaaggcg cgagctttat cagtatcccg caagatgtag tggatgcacc
     3841 tgtccaaggg aatgtcatca agccattgag cgatcctaaa ttaggttctg cttctgcaga
     3901 tgatatccgt tacctagctg aacgcattag agaagcaaaa ctaccggtac tattagtagg
     3961 tatgcgagga tcaagtgaaa aagaaacgtt agccatcagg caattagttg gaaagacagc
     4021 attgcctgtt gtagagacat tccaagcagc tggagtgatc tcacgtgaat tagaagcgca
     4081 tttctttggt cgtgtgggtt tatttagaaa ccaaccagga gatatgcttt tgaaacgaag
     4141 tgatctagtt attgctattg gctatgatcc aatcgaatat gaagcaagaa actggaatgc
     4201 ggaaaaagat gcgcggatca ttgtgatcga tgaagcgcca gctgaaatcg atccttttat
     4261 gcagcctgaa agagaattga tcggtgatat ctcagccact cttgatttat tgacaggttc
     4321 attagagcct caacaagtgt cagaagatgc aaaagaatat ttagcatctc ttcaagcaga
     4381 attaacggag cgggacattg ttcaatcaaa aggagaagca ggtatcttgc atcctttaga
     4441 agttattaac acgcttcagt caaaagtaac agatgatatg actgttaccg tagatgtagg
     4501 tagtcattat atttggatgg cacgccactt tagaagttat gaacctcgtc atttgttatt
     4561 cagtaatggt atgcaaacgc ttggtgttgc attgccatgg gcgatttctg ctgcattggt
     4621 gcgtccaaat acacaaatcg tatcagtttc cggcgatggg ggatttttat tctctgccca
     4681 ggatttagaa acggcggtac gtaaaaaatt gaatatcgtt catttgattt ggaatgatgg
     4741 acactacaat atggtagagt tccaagaaaa aatgaaatat cagcgggcgt caggtgtaga
     4801 ctttggtcca gttgattttg tgaaatatgc agaagctttt ggcgctaaag gaatccgagc
     4861 tacaagtgta gaggaattag aaaaagcttt agaagaagga tttgcgacag aaggacccgt
     4921 aattattgat attccgattg attaccgtga taatgagaaa ttgggagaaa caatcttacc
     4981 agatcaattc tattaaggaa gtgtcgacat gactgaaaaa atcttatacc agcacgggac
     5041 attaggagct ttgatggctg gcttgatgga tggaacagaa acgattgctc atatattaga
     5101 aaaaggaact ctgggattgg gaacattgca tgggcttgat ggtgaagtca tctttttaga
     5161 tggcgtagct tatcaaggaa gatcagatgg ctcagttgcc caattagatg gaagtgaact
     5221 taccccctat gcagcaatta cagattttac gccagatact agtttttctg tctcaaaaac
     5281 tgcagatgga gaacaattga aaaaagacat attagtaaga gaagccgggg aaaacctatt
     5341 tttggcagtg aagataactg gattattcaa aaatatgcac atccgtatca tgccgaagca
     5401 agaaaaacct tacagaagac tagcaaaaat ttcagagtct caaccagaat tccaacaaag
     5461 caatgtccaa ggaacattag tcgggttctt tacgcctgaa ttattccaag gagttgctgc
     5521 agcgggattt cacttgcatt ttatcgatga tacacataca tttggcggac atgtaatgga
     5581 ttttgaagtg gctgaaggaa aagttgagat tagccgcatc tcgtcccttg tacagcattt
     5641 tccagttgat gatccgactt ttgttcacga aacgattgat tatgctgatt tagcagcaga
     5701 aatccaacaa gcagaataga aaaagacagc gagatgcgtt caaggtgatt aacctgacgt
     5761 atctcgtttt tttgaggagg aaacctagcg tttacaaaaa aactgagcta taatcttctc
     5821 catagatcaa aaaggagcgg tttttatcat gtggacagaa aaacagaaaa aagatgcata
     5881 cgaaatttta ttattacagc aacaaggatt attagaagca gaagataact ggttagcaaa
     5941 tttagcaaat tcatctgcat tgttgaatga gactttgcct gagactgtct ttgcaggata
     6001 ttatttattc gatggtaatg aattgatttt aggaccgttc caagggcgag tttcttgcac
     6061 acgtatcaag atgggaaaag gagtttgtgg agaatcagct gaaaaacaag aaacattgat
     6121 cgttgatgat gtcaaaaccc atgcaaatta catttcctgc gattcagccg cacaatcaga
     6181 aatcgtagtg ccaatggtaa aagaaggtaa tttaatcggt gttttagata tcgattgcgg
     6241 agtaacgaaa gggtatgatg aaatcgatca agcatttcta gaaaaatatg tagatctttt
     6301 attagcaaat tttaatataa atgctttttc atttattaat aactaaaatc gtctattgaa
     6361 acaaaaaaga agagaaatca agaataaaaa gagccggaaa gacagtgggc atttctgttt
     6421 ttcggttttt ttattttttt ataaaatcac aagtgttatt gattttcata atcatatttt
     6481 aatggtatta tcattttaaa taatggaaaa acattaagga tggatgaaat atgaataacg
     6541 aaactatcat tttaggaaaa aaatatattt gccaacctat tggcttgaaa caaaaagtgg
     6601 ttggagaagt aatcagtaaa ttagaaaatt gtgttgtgat ctgcgtagac gaatatcatg
     6661 acgtcgatcg caatgaaata ttagaaaaat gcggaaaagt agtggcaaaa tatactcacg
     6721 tttatgagtt ggaaacaaca gaatatctat ataactatag caacaaatat gaacatactt
     6781 tagtttcata aagatttaaa taaagtgcct tcaaaagcga gaagaccaag cttttgaagg
     6841 cactttattt agttggcagt gattgttcat gatttttgat ttattcaaaa ggttcgccta
     6901 gagaatctgt ctctgtaacg aggcctggtg gagaaactcg ataaaaacct atactgccag
     6961 aactgtccta ttttcggata gacaaactag tagttttaaa aagatcatcc gatccaatca
     7021 tttcaaaaaa tgcaaaactg atatcaggat catttagctc aggcttgtta tcgtaaagca
     7081 tttgtaaagg ttgcatttag ttaggagtaa acgcgaaact atcatcatta tttgctgaag
     7141 aagatgaaga agctaattca gagtttgttt gaaataaagg agacgtgctg acagtttctt
     7201 taccctgttt tttgcagagg tagaaacttt tatatcggaa gtttctaccg gagaattttt
     7261 ctcatttgta gaaattgttg aatcgatact attcgaaatt tgcgtatctg aaggtatgtt
     7321 ctttgatgca gaagtcccac aagcagtaag taacaataaa tttgataaac tgataaacag
     7381 aattcttttc attatgtaca cactccttat agtatcgttt agcatacacc tatgctcagt
     7441 attaggcaaa atcaatgcaa aactatcaag tcttttatag tatctattag tcttagtaat
     7501 acatatatgg agagggtgat atcccagttc aaaccagata cattggttac catctgtatt
     7561 aacgattatg cctctaaacg ctttttttat gaaaaaattt ataagtgaaa acgtaggtat
     7621 gatattatct agaggtaatt tttttgggta tttaaatagt tgctaggagg ata
//
LOCUS       DACDNO010000095         7637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDNO010000095 DACDNO010000000
VERSION     DACDNO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.095"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            344..3178
                     /locus_tag="IWI22_002568"
     CDS             344..3178
                     /locus_tag="IWI22_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9169898.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLGTFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            3369..3860
                     /locus_tag="IWI22_002569"
     CDS             3369..3860
                     /locus_tag="IWI22_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9169899.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            3963..4952
                     /gene="manX"
                     /locus_tag="IWI22_002570"
     CDS             3963..4952
                     /gene="manX"
                     /locus_tag="IWI22_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9169900.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            4985..5788
                     /locus_tag="IWI22_002571"
     CDS             4985..5788
                     /locus_tag="IWI22_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9169901.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            5809..6723
                     /locus_tag="IWI22_002572"
     CDS             5809..6723
                     /locus_tag="IWI22_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9169902.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            6806..7180
                     /locus_tag="IWI22_002573"
     CDS             6806..7180
                     /locus_tag="IWI22_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9169903.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            7319..7564
                     /locus_tag="IWI22_002574"
     CDS             7319..7564
                     /locus_tag="IWI22_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9169904.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         2475 a         1263 c         1683 g         2216 t
ORIGIN      
        1 ataaaagttg tagtggaggc tacgaagttt actttgtctt tttacactta atattttttt
       61 gccaatttgg aatcaggatg ttttgataac tatcgttttc aattgatgga agctatattg
      121 tttgctatca gtgaaataat tatatggtaa acagtgttct ttttcttgtt tccattataa
      181 aaatcgtatc gggatttttc gttcttttaa attgaacatt tctatctatc cgcgtgttat
      241 caacaaaaca aaatcaggta aatagttgac gaaacagaaa taaatacata gtattatgtt
      301 ttgaagccta agtacaatag tctatagaaa aggagcgata ccattggcaa aacgcattga
      361 ccgaatttat atgtatgtca aagaaaaaac cgctcattta acaaaggcag aatatgatca
      421 aggagtaaca acacaggaaa ttgctgaagt gttagggatt cagcgaacaa atagcagtaa
      481 agatctgaat cagctagtgc gcgaaggaaa actgataaag acagacggac gaccagtacg
      541 ttacatatat caaacactcg ttacgcacgt aaagccagtt acaaaacatg tagtcagtta
      601 caaagaaacg ccggtctctg aagaaaaaaa tacactccaa atagatacta aggatatctt
      661 tgcaaaaatc attggagcta acggcagcat gaaaaattca gtggaacagg caaaagctgc
      721 gattttgtat ccgccaagag gattaaattg tttgattaca ggacctaccg gatcaggaaa
      781 gacttatttt gcacacgcga tgtatcattt tgcaaaagcg catcatgtaa tagatgagga
      841 aacggaactg attgttttca attgtgctga ttatgccaat aaccctgagc ttttgatgag
      901 tcatttattt gggtacgcaa aaggtgcttt tacaggcgca gatgaagaaa aaatggggat
      961 tatcgatcaa gcggatggcg gtatgctgtt tttggatgag attcaccggt tgccgcctga
     1021 aggtcaagaa atgatctttt actttatgga tcatggaact tacagtcgtc ttggggaaac
     1081 aacgaaatcc catgaagcaa atgtacggat cgtaggtgcg accacagaag acccaggttc
     1141 ttctctatta ggaacttttg ttcggcgtat cccaatcaat atcaaacttc cggcttttga
     1201 aaaacgtcct gcaaatgaga aagtcgattt agtaaaaatc atgattgccc acgaagcaaa
     1261 ccgcacgcag cgaaagattt ctttgacaga agatgtagca aaagcattga tcggcagtgt
     1321 gacctatgga aatataggac aattgaagtc aaatatccag ttagtatgtg cacgaggatt
     1381 tctaaatcat atgcaatcac ccgaaatctc gatcacaatc gatgatttga cagaagggat
     1441 tcgtagcgga ttgattcaat tagcaagcaa ccgcagtgca atggcagagc tttctaaaat
     1501 attggagcca aagataactg tagcaccaaa tgatacgatc atgaaaatcc aatcggactc
     1561 ctatgagtta ccttataatc tatatgatat catcggagat aaagcagctt tactgaagtc
     1621 ggatggtttg gatcaagaag caatcaatca tttcatttct acagatatca acatccatct
     1681 gaagtctttc tataaagacc atggtttttc ttttaatgca gacagcaaat tagcagaatt
     1741 tgtagatccg aaaatcatag aagtgaccaa tcagatttat gtaatggtca aaagatcttt
     1801 accatatgag tttcagcaaa actttattta tgctatgagc ttgcatatca gttctttttt
     1861 gaaaaaaatc aacttaggag aagaacggca tacgaatgat aacatccgtg agatggcttt
     1921 tgattttcca gatgaatatg ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt
     1981 tcaagtggag attccagaaa gtgaagacta ttacttgacc gttctactcg tctcgttacg
     2041 ggcaaatcaa gcaagcggca gaatcggtgt agtcgttgca gctcacggaa acagcacagc
     2101 aagcagtatg gtccaagttg tccaacagtt gttagatgca gagaacgtac gagcagtaga
     2161 catgcctttg gatatggatc cgaaaacagc attggaccga attgaacgca atgtacaaga
     2221 agtagatgaa ggaagcgggg tcatactctt agtagatatg gggtcccttg cgtcgtttaa
     2281 ttcgcaaatt caaagagaca ctggaattcc agtacgtact gttgatatgg taacaacttc
     2341 tctagttttg gaaactgtac gaaaggtctc agtattaggg acagatctag atatgttgca
     2401 tgaatcattg aaaaattttc gaggttacgc agaaatatct tctgtagaat ctgttcagga
     2461 agcaaaacct attcataaga aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac
     2521 agcacaaaga atcaaagaat tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt
     2581 agaagttcta gctttgtcga ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga
     2641 atatcagatc atagctacta ctgggattac tgatttgaat atccaagccc catttattcc
     2701 tttagaacgt tttattgatc aaaatatcga agtcattttg gatcagttgc tgatggaatc
     2761 agaattggag gaaactgaat tcatctccct agatgaagag tcagctaaaa atacgtgtgt
     2821 tgagtttatc tctgataact tcatctttat taatggtagt aagttaatcg atccaatgtg
     2881 gcaattttct acacaaatct ctcagacgac gggaatcggc gatgaagaat atggattcaa
     2941 aatcaaccta gtgatgcaca cagcaggaat gatcgaacgt attattcgga atgaaccatt
     3001 gactgtggaa gaaaacgaat tgacaaatac aacgaatgat cctctttata gccaattggc
     3061 agcctcagtt gttctgttgg aagatcagat aaaagtaaaa gtgccgatag aagaaatgta
     3121 ttatctgcta cgtctagtcc ataatcagtt agataaaaaa gaatacactg tgccatgaat
     3181 acacaaaaac gatacacttt tatagtgtat cgtttttgtg tattggttaa gaatccttta
     3241 aattcaatta gtgtatcgta aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta
     3301 ggaggtggtt gttcacacag aaatgatagg ggtttctgtg aagtagtgag gaagaaggag
     3361 ttttgcagat ggatattcga ttggcacgta tagatgatcg tttaatccac gggcaagtcg
     3421 caacagtttg gtcgaagcaa acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg
     3481 ctcaagataa attgagaaag tttctactaa aagaagctgc accgccgggg atcacagcaa
     3541 atgtaataac cgtgcagaaa atgattcaaa tctatcatag tggattatta tcacgagcaa
     3601 aagtgatgct tttattcacc aatccccaag atgttgaagc ggttgtccgc tgcggtgtac
     3661 agttgaagtc gttgaatatc ggcggtatga gttttactga aggcaaatca atgattacca
     3721 attttgtttc agtaaatcaa caagatattg attcctttcg ttacttgaac agtcaagaag
     3781 tggagttaga gattcgaaaa gtacctgctg acaggaaaat ccttttgatg gaactactag
     3841 cgaaaatcaa aaaggtgtag caagaatggt tgttggaaaa ggtcaaataa ttctgttgta
     3901 atggttagca attattaata tttttgaatt tgttacaaca aaagtaaata ggaggtataa
     3961 atatggtagg gattatccta gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg
     4021 gttcaatgat ctttggcgaa caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag
     4081 gtccagatga cttgagagcc aaattggaag aagcggttgc ttcttttgat aatcaagacg
     4141 aagtgttatt cttagttgat ttatggggcg gaacaccgtt caatcaatca aacacattat
     4201 tcgaagagca caaagataaa tgggcaatcg tcagcggttt gaatttacca atgttgattg
     4261 aagcctacgc atcacgcttt tcaatggaat ctgctcatga aattgcagca cacatcattg
     4321 aaacagcaaa agacggtgta aaagtaaaac cggaagaatt agaaccggca gaagcaccga
     4381 aagcagcagt tgaagacgca caacctaaag gagcacttcc agaaggaact gtggtcggag
     4441 acggaaaaat caaatatgta ttagcacgtg ttgactcacg tctgttacat ggacaagttg
     4501 cgacagcttg gacaaaagca gtgcaaccaa accgtattat cgttgtttca gatgcagtat
     4561 caaaggatga tctgcgtaag agactaatcg aacaagctgc tcctccaggt gtaaaagcaa
     4621 atgttattcc tattagcaaa atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca
     4681 aagcattgct tttatttgaa aatcctgaag acgtattgac agctgttgaa ggtggcgtag
     4741 acatcaaaga attaaatgtt gggtcaatgg cgcattcggt cggtaaagtc gttgtaagta
     4801 aagtattgtc tatgggacaa gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg
     4861 tgaaattcga tgtacgtaaa gtacctaatg attcacgcga taacatggat gatattttga
     4921 aaaaagcaaa agctgaatta gcaaaagcgt aataaataat attaaaattt ataggaggct
     4981 tatcatgtct attatttcaa taattttagt cgtattagtt gcctttctag ctggtatgga
     5041 aggtattctt gatgaatttc aatttcatca accactagtg gcatgtacat tgatcggttt
     5101 agttaccggt aacctcgaag ctggtatcgt acttggcggt acgcttcaga tgatcgcttt
     5161 aggttgggca aatatcggtg ccgctgtagc accagatgct gcattagcat cagtagcatc
     5221 agcaattatt ttagttttag gtggacaagg tgttaaaggt gttccttctg cgatcgcgat
     5281 tgccgttcct ttggctgttg cagggttgtt cttaacaatg atcgtacgta caatcgctgt
     5341 tccaatcgtt catttgatgg atgcggctgc tgaagaagga aacatccgta aggttgaaat
     5401 gtggcacatc attgctgtat gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt
     5461 gctatttatc ccagcacaaa ctgtgcaatc attccttgaa tcaatgccag catggttaac
     5521 agatggtatg gctatcggtg ggggaatggt cgtagctgta ggttacgctt tggtaatcaa
     5581 catgatggct acaaaagaag tatggccatt cttcgttatt ggtttcgttg tagctgcaat
     5641 ttctcaatta acattgattg ctctaggtgc tttaggtgtt gcattagctc ttatttactt
     5701 gaacctttct aaaatgggtg gttcatcaaa taatggaggc ggaagcaact ctggtgaccc
     5761 tttaggcgac attttgaatg actattaatc ttgaaggagg aaaataaaat ggcagaagaa
     5821 aaaatcaaat tatcaaaaaa agatcgttta gctgttgcgt ggcgctctac cttcattcaa
     5881 ggttcatgga actacgaacg tatgcaaaac ggtggctggg cattttctat gattccagca
     5941 atcaaaaaat tatacaaaac aaaagaagaa cgttctgcag cattaaaacg tcacttggaa
     6001 ttctttaata cacacccata cattgcttct ccaattttag gggttacatt ggctctggaa
     6061 gaagaacgtg cgaatggcgc gcctgttgat gatgtagcga ttcaaggggt taaagtaggt
     6121 atgatgggtc ctttagctgg tgttggtgac ccagtgttct ggtttacagt acgtccaatg
     6181 ctaggtgcac taggtgcttc attagctatg ggaggaaata tccttggacc aatcatcttc
     6241 ttcttaggtt ggaacattat tcgttgggca ttcatgtggt atacacaaga atttggctac
     6301 aaagctggtt ctaaaattac tgatgacctt tctggcggat tgttacaaga cgtaacaaaa
     6361 ggtgcttcaa ttttagggat gttcgtatta gctgcccttg tacaaagatg ggtatcaatc
     6421 aaattcttac cagttgtatc aactgtaaaa ttagataaag gtgcttacat cgaatgggat
     6481 aaattgcctt caggtggtga aggaatcaag agtgcatttg aacaagttaa taatggctta
     6541 gccctttctc ctgaaaaggt aacaacatta caagacaact tagaccaatt gattcctggt
     6601 ttagctgcat taggattaac attgttatgt atgtggttgc ttaagaaaaa ggtttctcca
     6661 atcgttatca tccttggttt attcgtagtc ggtgttgttg gacacgtcat cggactttta
     6721 taatccataa aaatatagct cgggcgttgt cccgagcttt ttttatatag tatactgttc
     6781 ataaacaaat atctggaggg agaaaatggt tcaatcaatc aatacaaaag tagacttggt
     6841 aattgatgct acttcgttta cgggtctgac agattacggg caaatcatga taggtgataa
     6901 tggttttgag ttttaccatg cgcgagatag acgcaaatat atccaaattc catgggaaga
     6961 agtggattat gtactagcat cagtcatgct aaagggaaaa tggattcctc gatatgccat
     7021 tcaaacgaaa aaaaatggta catacacatt ttcttccaaa gaacctaaaa aagtgcttcg
     7081 tgcgatcaga gaatatgtag atgaagatag aatggtacaa tcattgagtt tctttgatgt
     7141 agtgaaacgt ggtattcgta gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa
     7201 gaggaggttt tctttatttt tttactttgc agaagaaaat atgaaatagc aaaaccaatt
     7261 atttgtcccc tttaaatccc atatgctaaa attgattagc aaagaaggag gttatattat
     7321 gagttggtta tggacattga ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac
     7381 cagtaaagga aattcgatgg gctgtgtaac gaatatcatt gctggactag tcggttcaag
     7441 tattggacaa gcgattttcg gcgaatgggg accaaaagct gcaggtatgg cacttattcc
     7501 atctatttta ggcgcagtga ttttggtagc agttgtctca tttttctttg gaaaacgatc
     7561 ataagttttt acaaaatagt aaactaaaag gagttgcagc gagactgtga ctcctttttg
     7621 gctttattta tttaaaa
//
LOCUS       DACDNO010000096         7625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDNO010000096 DACDNO010000000
VERSION     DACDNO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.096"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(16..708)
                     /locus_tag="IWI22_002575"
     CDS             complement(16..708)
                     /locus_tag="IWI22_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9169905.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            793..969
                     /locus_tag="IWI22_002576"
                     /pseudo
     CDS             793..969
                     /locus_tag="IWI22_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1145..1714)
                     /locus_tag="IWI22_002577"
     CDS             complement(1145..1714)
                     /locus_tag="IWI22_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9169906.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="IWI22_002578"
     CDS             complement(1788..2111)
                     /locus_tag="IWI22_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169907.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="IWI22_002579"
     CDS             complement(2265..3077)
                     /locus_tag="IWI22_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169908.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="IWI22_002580"
     CDS             3431..4279
                     /locus_tag="IWI22_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9169909.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="IWI22_002581"
     CDS             4424..5365
                     /locus_tag="IWI22_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9169910.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="IWI22_002582"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="IWI22_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2273 a         1511 c         1372 g         2469 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacctattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaacgtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtgtg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcggca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 aacttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agttacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgagaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg aatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagcttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaac ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctcgaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaac
//
LOCUS       DACDNO010000097         7621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDNO010000097 DACDNO010000000
VERSION     DACDNO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.097"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(227..562)
                     /locus_tag="IWI22_002583"
     CDS             complement(227..562)
                     /locus_tag="IWI22_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9169911.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(546..818)
                     /locus_tag="IWI22_002584"
     CDS             complement(546..818)
                     /locus_tag="IWI22_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9169912.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1269..2072)
                     /locus_tag="IWI22_002585"
     CDS             complement(1269..2072)
                     /locus_tag="IWI22_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9169913.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(2050..3174)
                     /locus_tag="IWI22_002586"
     CDS             complement(2050..3174)
                     /locus_tag="IWI22_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9169914.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(3190..4005)
                     /locus_tag="IWI22_002587"
     CDS             complement(3190..4005)
                     /locus_tag="IWI22_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9169915.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3995..4876)
                     /locus_tag="IWI22_002588"
     CDS             complement(3995..4876)
                     /locus_tag="IWI22_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9169916.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(4952..6241)
                     /locus_tag="IWI22_002589"
     CDS             complement(4952..6241)
                     /locus_tag="IWI22_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9169917.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6385..6896)
                     /locus_tag="IWI22_002590"
                     /pseudo
     CDS             complement(6385..6896)
                     /locus_tag="IWI22_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6963..7106)
                     /locus_tag="IWI22_002591"
     CDS             complement(6963..7106)
                     /locus_tag="IWI22_002591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9169918.1"
                     /translation="MITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLILA
                     HDR"
BASE COUNT         2351 a         1632 c         1171 g         2467 t
ORIGIN      
        1 atgagaatta gaatcttttt gatcaatcaa ttaatcaatg taagataaca aaaaatctga
       61 accagaatgt ggttcattct gacccggatt tgattttgca caactcatca gtccttattg
      121 acaaagagcc aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta
      181 caacccatca gtccttattg acaaagagcc cttcttttta gtattattat aattcttcca
      241 tgcgttcttc cagtcgctgt aaatcgcttg gacttaattc cccaattttt ttccatttga
      301 cctttttgtt gtttttgggt aattcgttta gagttcctac gtcaatccaa gattcttttt
      361 ttagtcctgc ctgtttccaa tcccttattt caacgtattt cagctttatt ttatcggatt
      421 tagtatgata ttttgaagta attgacagaa acgaaattgt gtccagtcca ttttttagaa
      481 tatagacagg ccggcgttta ccgccctctg cagctgtgaa agcaacgtag acggttgcta
      541 tatcattagt attcatcttc attgaaccat tcttcaattt tttcgtcagt gtctagtttt
      601 tggacaggca atttttccgc taatctttga atattatgga ttcgtttact tttttccgac
      661 aattcaatag aaaatgggat ttttccttgc tcaacaattt gcttataaaa aacggtgacg
      721 gctgttttgg ggctcatgcc tagttcatca ataatgcttt ctgcttcatt tttcaatttt
      781 tcgtccatag aaatttgtaa cttagccatt ttctccacct ctttctgaca aacacattat
      841 atcacggaaa gagttcaaat ggaattaata caactacttt tgaactcctt gaaatgcagt
      901 gtatttatct aaatataagg aaaggatata gtgctaaatg gccttgatag atagaaaagt
      961 ttcaaaatcc gccacagcga taattgaagc gaacactcaa cgacacacag aacaggaaaa
     1021 gactcctaaa agaaccaaat taactaggac gtgtctgaaa actccacaaa gatgtggtat
     1081 gctctggtaa aaaacgaatg gcacaaccac gaagaccgat atggttcttg gaaaactttc
     1141 tccacccttt ttgtaattgg agggatagtg gtttattgat agcgattttc caagttcttc
     1201 aagtggaacc agacattgag caccacggct aaaaagtgaa tgaagtcttt atttttaatc
     1261 aataattttt aatgattacc agtaattggt tttttcatgc gccctatttc tatttgatgt
     1321 ggagttaaat aatgctgata agtaaaaatt tttccgtcaa ttaaactgcc actagtgaat
     1381 cctgttctat tggtaaaaag agaatagtct gggtattcat ctacaccaaa tgataaagat
     1441 tcagccataa actgtttttt accagcaaat tcagcactgc taccttgatg gatatggcct
     1501 acaaaaatac ctaaaatatc actttcggca atcaattcag caaaaccttc cggaacttca
     1561 gttacaatag atggatcttc ccacgacaat ggatgatgaa ccaataaaat cgttcccttt
     1621 tcagcaggtg tacccaacca ctcttgtaat tgaatcatct gtcctatcga tatttttcct
     1681 tcgtgctggg tatcttgagc agaatccaag gaaataattc gtaacccatt ttgattcgta
     1741 caatggatat actctcccgt ttcattgctt tcaacactca ttccttcaaa gaaaacactg
     1801 cgacgatcat gattcccacg acaaaaatag taaggtgtgg ttgggaaata ttgatccaac
     1861 attttccgta agacttgata atcagaaatc cccccttcat gtactaaatc acctgttaat
     1921 agaataaaat cgtacactgt taacgagagt tcttgtaaca atttttccaa tgccaactgt
     1981 ggatttttaa ttgttggaaa tagttcagca gtaaacaagt tagtttgata gttttctcta
     2041 aaatggatat cagagagatg caaaaagcgc atggctattc cctctttctt caataaaata
     2101 atcaggatat cctaaatttt cttgcgtctg ataatcaaaa aaatgcattc tttcaggttc
     2161 tatgcggaga ttgactgcat caccaatctg ccaagtaggt ttttcattaa ggacaatcat
     2221 ttctatacca tcgactttaa cggtgtacgc ataactttga cctaatagct cacgatactt
     2281 cacttgaccg tgtaaatcgc ttgtttcttt tgaaaaggtt acgtgttctg gtcttatgcc
     2341 taaatacaat gagccactaa cctgaagaag ttgtttttgc caagctggat tcaatgttaa
     2401 aaattgactg gctaccttta atgttccttg atcataagta actggcataa tattcatgcc
     2461 tggcgaacca ataaaatttg cagtaaaagt atttttaggt ttcgtataaa catttttcgg
     2521 cgtatcaatc atctgaattc taccttcatt caataaaaca atccggtcag ctaacgtcat
     2581 cgcttcaatt tgatcatgag tgacgtagac aaaagtttgt ttataacgtt gatgaatttt
     2641 taccaattct tttcgtgcat ccaatcgtaa acgcacatcc aaatttgata acggttcatc
     2701 taataaaaaa taattactcc gtttaaccac cgcacgagcc aatgctacac gctgccgttg
     2761 ccctcctgac aaatctttag gaagtcgatc ttggtatggc gttaaaccta acatcttcaa
     2821 agcttcttcc aaacggtgct gaatgtcagc tgtcttaatt ttattcgccc ttaatgcaaa
     2881 aataatattc tcctttacag tcatgtgagg atataaagca taattttgaa aaaccattgc
     2941 gatctctcgt tctccacaag gtagcttatt tgctagttgt gtacctaaaa aaagctctcc
     3001 actcgtaatt ttttccaaac cagcaatcat tctcagcaaa gtagattttc ctgaaccaga
     3061 tggtcccagt aacacaattc gttctccagc ggccacagct aaatcaattt cttttaaaat
     3121 ggtatgttct ccatattttt ttccgatatt tcttaattca agcgtttctc ccatgatttg
     3181 tcctccaatt tatttaatac ctgcagaaac aaaagtcccg ataatatatt tttgaaaaat
     3241 tgcatacaag actaacggta caaacattgt aattaaacta atcgccattg ctacggtaaa
     3301 atttgtccca ccttcagaac taatataggt ctgcatcgcc aacggcaaag tataattatc
     3361 tactgtccgt aaaattaata ccggccaaac atactcattc caagaggtaa taaaaaacca
     3421 aataccggta gaagtaatcg catgtctagt aagaggaaaa acaattttac tcatgacggt
     3481 tgtttgtttg atgttatcca atgccgccac atctagaagg ctcactggaa tttcccgcat
     3541 tgtttgcgtc aataaaaaaa tgcccatcgc atcagcaaat tgtggtatta tcacacccca
     3601 agtagtatca attaaattaa gttttgcaat caacaaataa ttaggaatca tcgcaacagt
     3661 aaaaggaatg aacatcgtac caatgatgat aaagtaccag attttcttcc ctttgattcg
     3721 gcaataagta aaactatatg ccgcaaaaaa agctacgcct aacttaacaa ccgttataat
     3781 tgtagcaata aaaaaagtat ttctcgtaat tttgataatt ggaagatttt cgaacaaata
     3841 gcaaaaattt tccaaggtaa acggctgtgg tatcaaacta gtaatcgtct gataggcatc
     3901 tttcaatggc ttaaaagaat tggccataat aaacaataat ggatagataa taatgattat
     3961 gatgaacata aataagagat gtggcaacca gcgtttaatt ttcatagtaa accccctttt
     4021 ccacaaattt aaatattacc cctaagaaaa cagcaaatat taataaagtt aaaactgaaa
     4081 ttgctgaaga ttctccgact ttatacattt caaaagcctt ttgataagca taatagatca
     4141 cgtttgcact accattattc ggtccaccac gagtgatgat cttaatcgga gtaaacacat
     4201 actgcagacc ttgaacaatg gtcatcacca ataaatatag tcctgtcgaa ctactcatag
     4261 gcaacacaat tgtaaaaaaa atgcgccaga tgggtacacg atccaactta gctgcttcga
     4321 tcatctcttg atcaattccc acaattgctg caaaaatgac aatgaaatta taaccaaaaa
     4381 ctttccaagc agtaatacct gcaatcacag caattaccca accttgtaaa ttcatccaca
     4441 taggcagtct taaaccaaaa tttcccaaca agacagcaat tggtcccgat accggattta
     4501 atatccatgt aaaaaccatc cccgccacta ccaaagaaat cacagctgga ataaaaaata
     4561 atggtttata caaattctgc cctttttcta taactattcc taataaaaag gatgtaatat
     4621 atgggaacac acagctcagc aagacaaata gaacggtata ggacaaagta ttcttcaaca
     4681 ctttatgaaa gactggatca gtcagcacct ttacatagtt ggcaaagcca acaaattctt
     4741 tagtgggggc caccatattc cagtcaaaaa aactcaaata gatcgtataa atcaatggat
     4801 aaaaaacaaa cacgcccaac aaaataattg ctggcgccag atacaaataa cctacatagc
     4861 gcttcatttc cgacagcctc ctcatcctta tgtcaaaagc agacgacctt gtttttcctt
     4921 tcttacaggc gctgtcgtct gcttgctttg cttatttgag taactcatta atttcctttt
     4981 gagtagcttt catcgtttca ttgacggaat tacctgataa aattttatcc cgcatatcca
     5041 acaatttttg ttcagcttct aatccagcat ctccaggaaa tgaactccaa ctgaccatct
     5101 tatccatctg atcaattgct ggtatcatca tctgattttc agctaagaaa gcttttaatc
     5161 cattttgggc gtctgcaaca tctttacgtg gtggtaaata accagtacct tctgtccatt
     5221 tagctaaaga ttcaacacta taaagatatt ttaagaaatc ccaagttgct tcctttttct
     5281 tatccgtttg tgatgtaaca gctaacattg cgccaccagc aggtagttta acttttttcc
     5341 cttcccaact tggtgactca actgctttag cagtaaaagt actatttttt tgaatgttgt
     5401 ccctttgagc aatagtggta tagaccatcg cgacgttacc atcaataaag ctttgcacac
     5461 cttgttccca cgtagtgtgt aatgccgtct ggtcttttac catcctttga tataattcat
     5521 aagcagcgat accatcttta gaagcaaagg tagctttgcc atcttttatt acacttgccc
     5581 cattactttc taaaattgct tgttgtgccc aattatcagc aggttcttga atatacacgc
     5641 catactttcc agttttttct tttattatct tagaaaaagt ttgaacttct tcccaagttt
     5701 ttggaccatt ttcatccaag cccgctgctt ttaatatatc ttgatttaaa tataaaaccg
     5761 gattactaat ggagtaagga atacccactt gtttaccatc tttttcagac agtgccatga
     5821 tattgtctaa aaaatgatct ttaaaataat cagcattatc cggatccact tcttctacta
     5881 aagattgagg attagtatat ttgaaatttt ctgcaaaata atctttaaaa gcccatccca
     5941 cttgtactac gtccggtatt tctttggagg caacagaagc ttgtaaattt tgcatcaagc
     6001 ctttatacat atcaggatta tattttgcta caaccttcac tttgtcttga gattgattgt
     6061 atttatctac taattcattc actgtttttc cacctagggt atccgcatta acatgccaat
     6121 actctatcgt tacacgaccc tccgattttt ttgttgtctt ggtattccca ctaccacaag
     6181 ctgccaaaga gatgacactt aaaacagaca caacccctat aacaagattt ttctttaaca
     6241 taacattaac ttcctcctaa tttctttatg gccatttttc taatcaagaa atattatgaa
     6301 ctaccaatgt aaatttctag ttaattttct gtaaataatt cgaaatttgt gcatttcggg
     6361 gtacagggtc actggacata tccatcaagc taagaataaa gcccccatat ggtcatgtat
     6421 cccatctcca aatctcttaa ctcctctgtt ttgattataa aatagtttat tctgtgctcc
     6481 tttcttccat aaataggccc atgcacaacg tgaggttata ttataaacac cacagcgcgc
     6541 aaggtactca atcgtttcta gttcattgac tgtccataca gaaaatgtca atcctagctg
     6601 ctgccccact tcacgggcca acttagttaa ccatccttca tgaatattga cgatttgaac
     6661 gctttgatag gctaatttct gtaaggtatc gatcaaatct gcttcggtaa tatccccaag
     6721 ttttttccct tcgaactgat tttcaatatt ataaaatata tgctttcccc aaatcgcagc
     6781 ccaagataat tgtacatagc cactaagaac aatttgtggc cagattgata ggtttgtgcc
     6841 agcgaaaaaa cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt
     6901 gccttctcat tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact
     6961 tcttacctgt catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca
     7021 aaacaaagct ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg
     7081 tcactaccag agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt
     7141 tctcccacac gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc
     7201 cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga
     7261 atcgtcgatt ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt
     7321 ttaacctact acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga
     7381 ggttctttct tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga
     7441 gacttactat cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca
     7501 tcttctcaca aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt
     7561 ggactgtcac tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca
     7621 g
//
LOCUS       DACDNO010000098         7597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDNO010000098 DACDNO010000000
VERSION     DACDNO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.098"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(135..425)
                     /locus_tag="IWI22_002592"
     CDS             complement(135..425)
                     /locus_tag="IWI22_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169919.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IWI22_002593"
     CDS             complement(543..803)
                     /locus_tag="IWI22_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169920.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IWI22_002594"
     CDS             complement(940..1353)
                     /locus_tag="IWI22_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9169921.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IWI22_002595"
     CDS             complement(1809..2294)
                     /locus_tag="IWI22_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169922.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="IWI22_002596"
     CDS             complement(2433..2648)
                     /locus_tag="IWI22_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9169923.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="IWI22_002597"
     CDS             2872..3672
                     /locus_tag="IWI22_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9169924.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="IWI22_002598"
     CDS             3691..5559
                     /locus_tag="IWI22_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9169925.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKVGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="IWI22_002599"
     CDS             5552..6043
                     /locus_tag="IWI22_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9169926.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="IWI22_002600"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="IWI22_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9169927.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="IWI22_002601"
     CDS             6603..>7597
                     /locus_tag="IWI22_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9169928.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1444 g         2233 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 atagaagaag ggatatataa agtaatgaaa catttcaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaag tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt tcccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DACDNO010000099         7347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDNO010000099 DACDNO010000000
VERSION     DACDNO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.099"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IWI22_002602"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IWI22_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9169929.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3191)
                     /locus_tag="IWI22_002603"
     CDS             complement(2886..3191)
                     /locus_tag="IWI22_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169930.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3336..4541)
                     /locus_tag="IWI22_002604"
     CDS             complement(3336..4541)
                     /locus_tag="IWI22_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9169931.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5235..5621
                     /locus_tag="IWI22_002605"
     CDS             5235..5621
                     /locus_tag="IWI22_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9169932.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5719..5910
                     /locus_tag="IWI22_002606"
     CDS             5719..5910
                     /locus_tag="IWI22_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9169933.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6424..6756
                     /locus_tag="IWI22_002607"
     CDS             6424..6756
                     /locus_tag="IWI22_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9169934.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6666..6869
                     /locus_tag="IWI22_002608"
     CDS             6666..6869
                     /locus_tag="IWI22_002608"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9169935.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            7053..7208
                     /locus_tag="IWI22_002609"
     CDS             7053..7208
                     /locus_tag="IWI22_002609"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9169936.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2461 a         1298 c         1046 g         2542 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagta tttgcatcaa cacttgcatt accgaagtcc ttattataga
      181 catctaaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcaataagtt tggtaagatc attatcagta attgaaccta
     1141 caccataatt ttcatgatct ttgcgccgtt caattgctaa atcagccact tttttccctg
     1201 attcaaaaga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagctga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggttcc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta agctcttttc aaacgtaatt accccattag
     1681 gagcagtttg tgtatcagtt gtttcagccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggg
     2101 tgcttggaga aatttcatct tgtgcttgaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaaccaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa tagttatttt cttagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa acttatgtta actagtttac ttaaagaatt tcatgcacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ctctttctta gattaaactt cagagtattc
     2581 aaatagcgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcattcctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt attaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgcttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3301 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattatcc
     3361 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3421 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3481 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3541 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3601 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3661 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3721 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3781 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3841 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3901 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     3961 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4021 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagatttcct
     4081 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4141 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4201 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4261 aaatctttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4321 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4381 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4441 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4501 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4561 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4621 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4681 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4741 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4801 aggaattttt cgtttctaaa tactccaatt tgggaatcca ataaaaattt tcgttcaaat
     4861 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4921 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     4981 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5041 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5101 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5161 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5221 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5281 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5341 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5401 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5461 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttaata
     5521 aaactgataa agaaattgga aaggttctgg gtattacaag acagggtgcg acaaatttaa
     5581 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5641 tttgtatgct tctttgctcg tcctcctcct tcccaaaaaa ataaatagat tataagttca
     5701 tgggaagaag gtaaatttat gaatacttta aaagaagatt tcattttagc aaagaatgga
     5761 aatgaagaag cagttgtggc cattctcaaa agatttagcg ctttggtcca taagcaatcc
     5821 tggagaactg gaaaatatga tcaagattgt tatcaagaat gtatgcttgc aatatatcta
     5881 gctatctcga aattcgaaat taaagaataa ctcaactaag ggagtctgtg acataagtaa
     5941 aaagatagtt tcaaatttcg aacaataaat ttgaagccat cttctttata attataaagt
     6001 atcgatttta cagcgttcta taatgattca tagaatctaa aacgaatgat tttaattatt
     6061 aacgtttttt aattcgtttt tagcttaaaa ataacttttg tcacagtctc actcctaaaa
     6121 ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc agaactgggc catggtactt
     6181 caacgaggac ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta
     6241 gatttattca actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat
     6301 tttggttgtt aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac
     6361 taagttgggc tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact
     6421 ctattgaaaa aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag
     6481 aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc
     6541 cgatgggttt cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga
     6601 ttacctgata aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa
     6661 gacgagtgtt ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt
     6721 acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt
     6781 tatgcacaag aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag
     6841 aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa ccaaattgat gctgtttggg
     6901 tgacggatat tacttacatt ccatgtaatg acggtcgcct ctatttgtcc acctatattg
     6961 acttagcaac tcgtattcta cgttactata tggtggattc acacatgaaa aaagagattg
     7021 tcgtccgtcc attacaaatt tattaaggag aattgcctac ggtaattcat tccgatagag
     7081 gtagccagca tcggtctttt atttatcaag agctgttgac cgaccaccac atcacacatt
     7141 caatgagcca aactgggact cctgttgaca atgcagtaat tgaagcgttt catcgatcaa
     7201 tcaaatgaga attaatcgac tccaacaaat ataaaagtaa aatagagatg aaggtgttaa
     7261 tccaagatta tttagaagac tactacccaa acaaaagaat ccatacaaaa tttatgatga
     7321 ctccttgaca atttgaagcg aagaaaa
//
LOCUS       DACDNO010000100         7158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDNO010000100 DACDNO010000000
VERSION     DACDNO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.100"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            236..1627
                     /locus_tag="IWI22_002610"
     CDS             236..1627
                     /locus_tag="IWI22_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9169937.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1665..2495
                     /locus_tag="IWI22_002611"
     CDS             1665..2495
                     /locus_tag="IWI22_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9169938.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2467..3456
                     /locus_tag="IWI22_002612"
     CDS             2467..3456
                     /locus_tag="IWI22_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9169939.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            3570..4565
                     /locus_tag="IWI22_002613"
     CDS             3570..4565
                     /locus_tag="IWI22_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9169940.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            4569..5711
                     /locus_tag="IWI22_002614"
     CDS             4569..5711
                     /locus_tag="IWI22_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAP9169941.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            5708..7135
                     /locus_tag="IWI22_002615"
     CDS             5708..7135
                     /locus_tag="IWI22_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9169942.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
BASE COUNT         2558 a          931 c         1260 g         2409 t
ORIGIN      
        1 tatgaatatt gtaacttgtg aataaggaat tagcaggaga atactagttc agcataaagg
       61 aagtgatggg acttctgaga caactttctg ttatgcagaa atacacatat aaacagacaa
      121 taaacgcttt tccccgtttc ttttaatttc ctttacaatt tggttattca ctaggttttt
      181 aaactttgct atggtaagat gatagaggca ttattttatg gttaggagtg tatatatgaa
      241 taaaaatggg gagtggaatg cggcacgcag aatattcatt attcttatgg atgtacttgt
      301 attaaatgta tcgatctatc ttagtttttt gctgaaattc cagggagaag taccagtgag
      361 aaacttagaa acctttcagc attcagcaat ttttatttcc atcatcttta ttgcgttgaa
      421 tattttgtta ggagcttatg tattctataa ccgaatgatc agtgatattg tcttcattac
      481 tgttattgga caagtactaa tagcattggg gatcatggta gttacttttg ctggccgatg
      541 gtttgcattt ccgcgtggcg tcattttgat ctcgtttatc ctaggtacga tattacttag
      601 tgtttaccgg atagtcgttt acaaactgta cttaagagtc agtcgtgata agaaagtcct
      661 tatcgtgggt ttggagaaag atgtcgcacc agctatccaa aattttcaat ctaagaaaaa
      721 caataaacat aaggttgaag cagtggcaat cgataattat tacgaaaata caaaaaaatt
      781 gatcgatgat atggatatcg tctatttggc ttctaaaatc ccagaagatg aaaaaatcaa
      841 catttatcaa ttagtgacta aaaaagagaa gaaattattt ttaaatacga cttttgagaa
      901 tctgacaatg ataaatccga atattatgaa ttttgaagat gaaagcatca ttgaagcttc
      961 tgggttcaga atccaaccag aatacgaact attcaagcgt ctctttgata tcatcgtttc
     1021 gcttatttta ctgatcctcg cctcaccttt tatgctaatc acagcgattt tggtaaaaac
     1081 aacttcacct ggaccaatca tctataaaca agtacgtatt acgaaaaatc aaaaggaatt
     1141 ttcaatctat aaattccgtt ccatgagtgc gacagctgaa gcaaaatcag gaccagtctt
     1201 ggctaaaagt aacgatgcac gtgtgacacc agtaggtaaa tttatccgtg ctgtacgttt
     1261 tgacgaatta cctcaaatct ttaatgtatt gaaaggcgat atgtcgattg tcggtccaag
     1321 gccagaaaga ccatttttcg ttgatcagtt caatgaagaa aatccttatt attacttaag
     1381 acacaatgta cgtgcgggaa ttaccggata tgcacaagta tacggaaaat atgtatcaga
     1441 ttataacagt aaactacgtt ttgatttgtt gtatatcaaa aagtattcct tactattaga
     1501 tttgaagatt ttacttcaaa caatcaaaat cttatttgat aagatgtcct cacaaggact
     1561 agatgaagat gaaaatatca gttcattagc agaaagtgca tttccaaaag aaaaaacgta
     1621 tcgttagatt ggaaaaattt tgagcaaaga agtaggagag aacattggaa aataaaaata
     1681 taagtatact gctttctttg tatgataaag aaaagccgga atatttaaag gaagcattag
     1741 ctagtatctt tgagcaaacg gtgatgccgg cagaaatcgt attggtttat gatggcccta
     1801 taaatgagaa gcttcaggca gttgtcactt atttccagca aaaagttcct cacttgttta
     1861 cagttgtaaa attagaaaaa aatcaaggat tggggattgc tttagcaact ggattagaac
     1921 atactaaaaa tcgcttagtg gccagaatgg atacagatga catcatggaa ccacacagat
     1981 tggaaaaaca attagagatc ttccaagaat atccagaaat agcgattgtt gggagtaata
     2041 ttgaagaatt tactggcaca cttagcaatg ttatcggaag aaggattgta cctgagtata
     2101 atgatgaaat ccgtgtgttt tcgaaaagaa gaaacccttt taatcatatg acagtcatgt
     2161 ttgataaaaa tgctatttta gaagtgggaa actatcgccc attaattgga tttgaagatt
     2221 attatttgtg ggtaagacta ttgaaggcgg gatataaagg atataatatc caagagattc
     2281 tagtacatgc tagagcaggt gctgacatgt atgctagacg tggaggaagt aaatatcttt
     2341 taccaggaat taaaggccga tatcgcgtat ggaaagatgg tctaggatca ctaaaagatt
     2401 ttctgatagt tatttgcgga catcttgtaa ttagtttatt accgaataaa attaggggaa
     2461 aagtatatga atcaaaactt agaaaaacaa actaaacttt ctataatcat acctttttac
     2521 aatgcaaaga atactttaag aagagctgta gacagcgtct taacgcaagg ttatcccaat
     2581 attgaaatct tgttaattga tgatggatct acagatggta gtagtgacat tggtgacgaa
     2641 attgctaatc tagagagtaa agtagcatgt tatcacttgg aaaacgctgg tccatcagtt
     2701 gctaggaact atgggataaa agaagcaact ggaaatttgg taggattttt agatagtgac
     2761 gattattttt tacctgattg ttttaataat gttttgaaag actaccatag gaatcacccc
     2821 gatatcattg cttttggatt aagtaaagga aaaaatatag aaaatgcaaa tttatttgtt
     2881 cccgaaaaga agattggttg ttcaagtgaa gaagcaattg agcaaatgtt tagaagtaag
     2941 gcagtggact tttacgtatg gaataaattt tttaaaaggg aattattcaa tcatatagtt
     3001 tttccagaaa ataaattata tgaggatatg gttcctatgt atgaagcttt tagggcggct
     3061 agtaaaattg atattttgcc attttcaggt attttttatt atcaaaataa cgatagcatc
     3121 gtttatcagt cttttaattc aaaacagtat gataatattt tgcagagaaa aatattagtt
     3181 gatgaagtaa agaaagatta tcctcgactt attaatttgg ccaatgctcg gttagtagac
     3241 ggatatcttt caacagggtt taaaattact ggcaaaaatc aaaatcagaa agaaaaacaa
     3301 aaatattata atcagtctaa aagagaaata aaaagtatat attcaaaaat aagaagtaac
     3361 aatgagatat caattgctaa aaaattagct ttatttttat acttaattaa tcctaaaact
     3421 tattttattt tatataaaaa agttctaaaa aaataaagtt tttttcgata tgttattttt
     3481 tatgtttatt aacttcggaa gactaaaatt gttactggta atgatacatt tgtaaaaata
     3541 aaaaagatga tttttaggaa gtgaagagag tgcataaaat tagtgttata gtacccgcat
     3601 ataacgtaga aaactattta gaaaactgct tagaatctat ttccaatcag acacattcaa
     3661 atattgaaat aatagtagtc gatgatggtt ccccggatcg aagtggggaa attgcagaag
     3721 aatataaaaa gaaagatatg cgattaaaag tgatacatca agtgaatggt ggtttaagtg
     3781 cggctaggaa ttctggtctt gattctgcaa caggagattt tgtgtgcttt attgacagcg
     3841 atgattggat agaaccagac tatcttgagt tactatattt tggtacgcaa acttatggag
     3901 cagatatctc ggtgctaaaa ataaaaaaga tttcaagttt agaacaacta aaagaacata
     3961 caattgctga gagaaaatgg gaaatatttg atagagaaca agcaatgaaa aacttattta
     4021 taaataataa aattggctat tctgcaaata ataaattatt caatagaaat ttatttgata
     4081 atataagatt cccactgggt aaattaatgg aagataaggc tactacttat ttgttgatcg
     4141 atcattccga aaaagttgct gtcaattcat ctgctaaata tcactattac caaagcccta
     4201 aaagtatact gagaggaagc ttcaatccta gaatttttga ttcttttgaa gtccatgaag
     4261 atatactagt attcatagat aagttttatc cggaattatc aattaatgtt agaactcgct
     4321 atgtctatga agcaataagg atgatgatga caatgattaa aaataactat accaataaag
     4381 aagattattt acgttctata aatattttac aacgctttga aaaagaagtg aaaattgatc
     4441 ccgacttttc accaaaatta aaaaaattag taaagttttt attaataaat aaaaacttgc
     4501 cttactatat gtcaagtagt aagatagcaa gtaaattttt gattaagata gaatctgtga
     4561 ggtaagagat gaagaatata aaaaaatttt tttcaaggaa tattttcccg gtttttatta
     4621 aaatattacc tattcaaaaa aatttaattg tattttcaag ttttggcggg cgtaattatt
     4681 ctggaaatcc aagagttatt tcagagaaaa ttcatgaaaa aaaattgaat tacagaatta
     4741 tttggttaat caaaaaagaa gcgattataa ctgatttacc agattatatt aaaagaattg
     4801 attccgattc ttttttagct gccttttatt tgtatacagc taagtattgg atagacgata
     4861 gtcggaaaac gattaagtat gcaaaaagga aaaaacaatt ttattttcaa acatggcacg
     4921 gtacgccttt aaaaaaaatt gaatttgatg caaaagataa gttacctaaa cgttacctaa
     4981 gctatgcgaa aaaagatagt gagtcaatta catatttatt atctggtaat cgctatagtt
     5041 ctgagaagta tgtttcagca ttcaatatcg ttccttctaa aatattagaa gtaggaactc
     5101 ctagaaacga tgaattagcc tctagtaaag agcaagtgtt tgacttgaaa aagaaacaaa
     5161 tcaatgtctt atttgctcca acttttcgaa ataatattga ggacaacgga attacgcaac
     5221 tagaatggtt aggtgtggat aacttacgtg aattttttaa aaagcaacaa caagaattaa
     5281 atttaatgac taaatttcat cctaatgttc atagtaaatt agcaactgat tctaaaagta
     5341 tagaatatat gaaaaaacat caaattactt taattaatga tgggattcct cttgaaatgc
     5401 tttttgaaga gatagatgtt ttaatcacag attattcatc tatttttttc gattttgcgc
     5461 taacaaaaaa accaattatt ctttttaact atgatgaaaa agaatatagc ttagaaagag
     5521 gtttttatat acagttaagt gagttaccaa ttataagcgt acaagatagt tatggccttg
     5581 ttgatgtttt taaaaaccaa caagaaaagc ttttgaaaag ttctcaagag ttacttgatt
     5641 atattggaaa ttatgaaaca ggggcaagta cagaaaaggt tcttgagttg ttagagaggg
     5701 atataaagtg aaaaaaacag taaaagatat attttataat gcgatatatc aagtcttttt
     5761 gattgttttg cccttactta ctatacctat acttagtcgt agaataggtt caactggttt
     5821 agggatttat gggtatgttt tctcaatatc acaattttta atgactgtca ttgctgtggg
     5881 aatgaatcct tttcgtattc gaaatatcgc taaatcaagg aaagataaaa aagcactatc
     5941 tttacaattc tggaatattt actttcttca atttttaata ggcttgagtg tttcctttct
     6001 ctatatagcc tatatttatt tttttgatgt aaagtataaa aatttatact taattcaatt
     6061 gatatttatt gcagggctaa cattagatat ttcttggttt tttcaaggaa ttgaagaatt
     6121 ttcgaaagtg gttattagaa atacagttat caaattatca tcggtattgt taataatcct
     6181 ttttgtttca actaaagatg atttgtggat ttatgtgttc attacttcaa ttactaactt
     6241 tttaggaagt ttagttttct ggttgagtat ccacggaaaa gtaggtaaac cactctttaa
     6301 ctctaatgtt tttaaacagt tgtggaaacc agcttttatt attttgactc ctcagttgtt
     6361 tatgcaagtg tacacaactt tagacaaaac gatagttggg tattttgtgg atccaacagc
     6421 attatcttat tatgatcagt ctcaaaaaat tgctcgtatt atattggcag tcttaacatc
     6481 aattactatt gttatgttgc ctaaaatatc taaagcacag gcagagggaa aatcagaaaa
     6541 tattttagtt tacacaaaaa aatcttttga ttacacgatt attattgcat taattctttt
     6601 tagtgtggtg tttatcaata caaaagaatt tgttccatgg ttttttgggg agaatttcaa
     6661 accgatgaca ataaacatgc ttatttcttg tctaattata gttgttagtc caattggtgg
     6721 agtttttgct aaccaatttg caattgctat agaaaaagat aaggagtatg cttatccaat
     6781 gatcattggt gcgattgtaa gtattattgg aaatgtagct tttgtgccta cttatggcgc
     6841 tgtagcggct acaatcgtac tcgtggtagt agaattatta gtctttttct ttagagtatt
     6901 ttttgtacga aaagaaataa aaatatccta tttctttggg aagaacgttg tcttattttt
     6961 tatagtcaat cttactttga ctacttcggc ctattacttt cttccacaat tattctcttc
     7021 ttcattttta gatatggcaa ttaaatcaat aatgattttc attatttatc ttccgtttgt
     7081 ttattttctt tttccagata tacgaaatga agtaagaaag attattcgaa aataaaataa
     7141 tgttgtaaaa aagtagtt
//
LOCUS       DACDNO010000101         6822 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDNO010000101 DACDNO010000000
VERSION     DACDNO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.101"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            134..1108
                     /locus_tag="IWI22_002616"
     CDS             134..1108
                     /locus_tag="IWI22_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9169943.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1090..1551
                     /locus_tag="IWI22_002617"
     CDS             1090..1551
                     /locus_tag="IWI22_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9169944.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1601..2065
                     /locus_tag="IWI22_002618"
     CDS             1601..2065
                     /locus_tag="IWI22_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9169945.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2079..2828
                     /locus_tag="IWI22_002619"
     CDS             2079..2828
                     /locus_tag="IWI22_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9169946.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2828..3658
                     /locus_tag="IWI22_002620"
     CDS             2828..3658
                     /locus_tag="IWI22_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9169947.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3658..4305
                     /locus_tag="IWI22_002621"
     CDS             3658..4305
                     /locus_tag="IWI22_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9169948.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4403..5179)
                     /locus_tag="IWI22_002622"
     CDS             complement(4403..5179)
                     /locus_tag="IWI22_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9169949.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5261..5404
                     /locus_tag="IWI22_002623"
                     /pseudo
     CDS             5261..5404
                     /locus_tag="IWI22_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(5521..6699)
                     /locus_tag="IWI22_002624"
                     /pseudo
     CDS             complement(5521..6699)
                     /locus_tag="IWI22_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT         2244 a          997 c         1236 g         2345 t
ORIGIN      
        1 cattttatta tataaaattg ttataaaaaa ttttttttga aaaatagtat tgcaaaataa
       61 agcaatttca tagataatag gtatcgtaaa gttatataac tttataatac ctattagagt
      121 gagggataaa gaaatgagac aactacacaa agaacagatt gaaacagcaa tggcagcgtt
      181 aaaggaaaag acaattgatc atgtatattt tacagcatgt gggggttcac aagccgtttt
      241 aatggctggt caatatctat ttgataaaga attaactgta ccttgtcatg tttatactgc
      301 taacgaattt aactatgcta taccaaaagg attatctgaa aattctttag ttatcacttg
      361 ttcccattct ggcacaacac cagaaactgt agaagcaaca aaaaaagcaa gagaatttgg
      421 agcaatgact atttgtttat ctaatgaaga aggatctcct ctatgggaag cagctgaata
      481 tcctgttcac tatgattggg gtaaagatgt tgatgctagt gatttaaata aaggaattct
      541 ttatggttta ttatttaata ttttacttgt tttagatggc tctaaaaaat gggaaacttg
      601 cttgaatgaa tttgataagt taaatgatct tgttattgct gaaaagagca aatttgaaat
      661 aactgctaaa gaatggggta aaaaccaaaa acgagataaa attatttaca caattggtag
      721 cggtattaat tacggagaaa tttactccac agcagcttgc tggttcatgg aaatgcaatg
      781 gattaattct aatgctattc attctgggga atttttccat ggtccttttg aaatcactga
      841 ttatgatgta ccattcatct tagtgaaaag tattggagat acaagatttt tagatcaacg
      901 ggtagaagac ttcactaaaa aattcagtaa taaattaatg gttttagacc aagctgattt
      961 tgacttagaa tctatagata gtgtagcgaa agaatatatt gcagctgtac ttagtggcgt
     1021 tgttattcgc caaatggtag aagcgattgc ttttgaaaga ggccattcct tagatgttag
     1081 acgctatatg tggcaaatga aatattaatt aataggaggt tgcaaaagat ggcaaatcaa
     1141 ttatttcaca ttgtgattac aacacattct accctttgtg agggttattt aaaggcaaca
     1201 aaattaattt taatgaatga cgccacaggt attagtgctg ttccatttaa agaaaatact
     1261 agtccagatg attttgagga gagtatgaaa gaaataatta gtcagcatca tttgcaacct
     1321 ctaattattt taacggattt aattggagga acacctaata atgtttctat taaacagttg
     1381 aacaacaata gaattcaaat tattgctggg attaatttac ctcttttact ggagttatta
     1441 atggcccagt cagctggtaa atctatagaa gaaatagatt ttaaggaatt actgaataac
     1501 tctcaaaaaa gtatggttca tattaattcg cttttaaaag atgtcagtta aggtggcaat
     1561 ttctattcta taaattttaa aaattgaagg aggaaataga atgattaaat tagtgcgtgt
     1621 agaccacaga ttacttcatg gacaagtaat attttcttgg actaaacagt atgatattaa
     1681 ccatattatt gtggctgata acaaagttcc aggagatcca atgtctgtga tggctttgac
     1741 aattgctaaa cctgctgatt gcaaattaga cattgttaaa attagtgatg ttaaagggat
     1801 tatggaaaca tccccaaatg ataattacat gattttaatt aaaggaccta aggaagcttt
     1861 tgaattagtt caaaatttac cagaaattaa agaaattaat tttggcggtg tgtctaagaa
     1921 aagcgagtcc aaacaatatg gaaaggcagt ctttttaact cctgaagaat tagaagccac
     1981 aaaaaaaatt cttgatcaag aagtgaaaat atttgtgcaa caagtcccat cttcaagtat
     2041 tgaaaatgta gattttagta agtaaggggg gaaaataaat gttattaaaa gccattttat
     2101 taggtttagt tggtgtggta gctgttattg actccaggtt aattggtcga caaaatattg
     2161 gtagaccact aattttgagt acacttgccg gacttgtgct aggtgacttg catacaggga
     2221 ttgttcttgg ggcatcttta gaattaattt ctatgggatt cgtctcaatt ggggcggctg
     2281 gacctcctaa tatgcaactt ggtagtatta ttgccactgc ttttgcaatt ttaactaata
     2341 gtagtacaga aacagcctta acaatagcta tcccagtggc tgtagctggt gaatttatga
     2401 gtattatcat gcgtatgata attgctcaat ttggacatgc tgcagataaa gccattgaaa
     2461 ataataaatt tagaaaagct caaattattc atatttattg gtcatttatt tttaatgctt
     2521 ttgtttactt tattccaatc tttttaacag tatattttgg tgctgacgtt gtggcagatt
     2581 tagtagataa aattccagaa atgattacag acgccttgac tgtctctgga aatatgcttt
     2641 ccgcattagg gtttgcaatg ctattaagca caatgttgag taaaaaatta tatccttact
     2701 ttatttttgg attctttatt gttgcctatt caggtctttc actaattggc gtgacaattt
     2761 ttgcagctct gattgccttt gtaatggatc aagttaaata tggaaaaaga gaagaggcgc
     2821 atggttaatg gaaaatagta cagaaaaaaa acgtttaatc acaaaaaaag atcttcataa
     2881 gatgtgtctt cgttcatgga caatcatgtg ttcgtggaat tatgaaagac aaatgcatat
     2941 gggatttatg tatgggatgg cgccaatttt aaataaagtt tataaaaatg atgaagagaa
     3001 aaaaagagaa gcttatactc gtcatatggc attttttaac tgtacgcctc aaatgactcc
     3061 atttattatg ggactttgtg cttctatgga agaacaaaat gctcaagcag aagaaggaaa
     3121 ctttaatact gaatctatct ccatgattaa aaccagttta atgggtcctt ttgctggaat
     3181 tggtgattca tttttccaag gtactttaag aattattaca tttgggattg gattgtcttt
     3241 tgctaagcaa ggtagtattt taggaccgat tttagctgtt ggcctttttg ctataccttc
     3301 tattttagct gttttttacg gcggtaaatt aggatatgag tctggcaata aataccttac
     3361 taaattgtat caagaaggtc ttatggatcg agtaatgcaa tttgcttcta tcattggttt
     3421 ggctgtagta ggtggaatgg tggctagcat ggtgccaatt gtcacccctt taaaattcac
     3481 tacaggggga acagaacttg ttttacaaga cttacttgat tccattattc ctggattatt
     3541 accacttgta tgtactttgg gaatatataa attgattaag aaaaatgtta gtacaaatat
     3601 gctcttatta ggttgtattt tggcagggtt tgttttaagt ataataggta tcttataatg
     3661 attaaggctc taatttttga tatggacgga gttttaattg atagcgagat tatgtatcaa
     3721 aagtgggtat ttaatttttt aaaacaagaa aatttaagct atcctttgta ttcttatcaa
     3781 aaaaagattg gaactacagc aagtattttt gatgatatag aaaagtacaa tgagggatgt
     3841 aacagtttag aattgaaggg aaaatttact aaatattgga aatcgcaaaa gattgattac
     3901 ttggatattt ttcctaatat actaattgaa cagcttgaat ggttaaaaaa gaaaaaaata
     3961 aaaatggcaa ttgcttcttc cagtccaatg gatactataa atgaagttgt taccacttgt
     4021 aaaatagagt catattttga ttgtttaatt agcggaagag atttgccaga aagtaaacct
     4081 aatcctacta tattccttaa agcttcagaa cagctcagaa ttccagttga agaatgtctg
     4141 gtgatagaag attcttataa tggtataaaa gctggtaaaa gagcaaatat gaaggttctt
     4201 gcaattaaag ataaacgttt ttctcaagat gtaagtctag cagatgatgt tatttacgat
     4261 attaaatatc tgcgacaaga agtacaggtg aaatttggaa tataaataag ttttttgatg
     4321 tatttagaga atattagtaa aaataaaaat catgcccaaa tctaatcaaa attgattgct
     4381 ttgggtatga tttcttttaa tatcaatcta ttactttagg atctgaatca tttttatgca
     4441 ccgtatctaa agtgatattt tctgtgactt caataacata actaattcta tcacaacgaa
     4501 ctgtggattg agaataatga aatggttgat tatttttatc tttgccaacc ttttctaaaa
     4561 taaatagcgg atctcctact atacattgca ataagtcgga aagctcaggt gttgcagata
     4621 aaccgtttat ttccataata gagctcgtag ctttgacatc atagtcatct tctagaagtt
     4681 tatataatga agtatattgg ctaccttttt caaataaagt tggaaatttt tcttctgatg
     4741 cgaaaaaatt atcaattccc atcggggcac cgtcttccca cattaaacgt ttaatattgt
     4801 aaactctatc agttacattg atttgtaatt tattagctaa aaactcatca gcctttataa
     4861 ttttagaatg taaaacagta tatctaattt tatgtcctcg ggtagaaaga gaatcgctaa
     4921 atccatgaac tccagctaat tttaaacttt cttttttttg agttacaaag gtccctttcc
     4981 cccttaatcg ttcaactgtt ccactttctg ttaaaagttt aaaagctttt cgaatggtaa
     5041 tcttacttac tccataaatt ttacataatt cttcttctgt aggtaattgc tctcctggaa
     5101 gatatttttt ttcacgaata tcgttacgta tagaagcttc tacttgtaga tataatggtc
     5161 tattatcttg aaaattcata gtacactccc taaaaagtta tacttcatta tatcacaatt
     5221 tattaaaata aaagattaag atataaatat aaatgaacta gtctttgact aaccaatgcc
     5281 cgcttttgaa agaaaacaaa ttcattctgt tttaagtaaa aatccttatg ttaccactca
     5341 ttctgaaggg gatgagcctt atcgctactt ggttgtggaa ccaagcgata agacaatttt
     5401 ataaattcaa tattaattaa aataaaaaca aacaaagtag attattttac tttgtttgtt
     5461 tttttattgg gagcgtcaat aattttgtgt aaataactcg tccttctgca aaataatggg
     5521 ttactcagta aacattgaag ctaatgtatc cgtcacttgt tggaagcctt tatggcttct
     5581 atttaagaat ttttgattgt atgtatcaaa gatggatact aggaaacgct ctagtgattc
     5641 ttcattttga aactgctctt ttctgcggct gtatttcttg atttgtttct tgaaagattc
     5701 gattaaatta gttgagtaaa tgcttcggcg gataccaggc ggaaactcat aaaaagtcaa
     5761 taaatcttga ttttttatca gtgactgcgt cactttagga taagtttttt gctacttctc
     5821 aatcatactc cctaaaaagg tatttgcctc ttcctttgag ctagcttgat aaacagcctt
     5881 aaaatcatca cagatttctt ttcgatcttt gacacgtact ttatgagcaa tattacggga
     5941 aacgtgaaca cagcaatgtt ggtatttagc tttaggataa atctggtgaa tcgtatcttt
     6001 catgcctttt aagccatcgg taataaacag taagacatca tgaacccctc tggagtaaat
     6061 gtcctgtaac aactcattcc aaacgtaggt cgattcagtc ggagcaatcg cataacttag
     6121 tacttcttta gtgccgtctt ctcgtatacc aatggcaata tagattgctt ctttggagac
     6181 ggtttgccgt tttaaaggaa tataagtagc atccatgaaa atagcgacat acttatcatt
     6241 tagagttcta actttaaatg catttacttc ttcagtcagc actttagtca tgttggacat
     6301 ggtttggggc gtgtagtgat gcccatacat tttctcaatc aagtctgcga tttccgacat
     6361 ggtaatacct tttcggaaga ggtgaatgac ggtctcctct aaggtatcat tcgtccgtct
     6421 ataagcagga acagtctgtt gcttgaactc gccgttgcgg tcgcgcggaa tctggagatg
     6481 aagttccccg tactcggtct tgaccgtacg gtcataggag ccgttacgag aattccccgt
     6541 gttaaaacca atgcgatcgt acttttcgta atcgaggaaa gccgttaact ccgttttgag
     6601 aagcgtattg acagcttttt ccaaatggac acggaataat tcattcaaat cgcctttgtt
     6661 cgctagagtc tttagaattt ctgtagtaaa atcgttcata tggaagtcct cttttctgtg
     6721 aatgtgttgt ggtaactcta ttctacagaa gggacttcct tttttctatt tacacaaaat
     6781 attttacact ctcgaaaagt attgcatttt attatataaa at
//
LOCUS       DACDNO010000102         6591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDNO010000102 DACDNO010000000
VERSION     DACDNO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.102"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(7..612)
                     /gene="dhaL"
                     /locus_tag="IWI22_002625"
     CDS             complement(7..612)
                     /gene="dhaL"
                     /locus_tag="IWI22_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9169950.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(640..1623)
                     /gene="dhaK"
                     /locus_tag="IWI22_002626"
     CDS             complement(640..1623)
                     /gene="dhaK"
                     /locus_tag="IWI22_002626"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9169951.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(1625..2020)
                     /gene="dhaM"
                     /locus_tag="IWI22_002627"
     CDS             complement(1625..2020)
                     /gene="dhaM"
                     /locus_tag="IWI22_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9169952.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            complement(2110..3069)
                     /locus_tag="IWI22_002628"
     CDS             complement(2110..3069)
                     /locus_tag="IWI22_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9169953.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            3239..3559
                     /locus_tag="IWI22_002629"
     CDS             3239..3559
                     /locus_tag="IWI22_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9169954.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(3671..4540)
                     /locus_tag="IWI22_002630"
     CDS             complement(3671..4540)
                     /locus_tag="IWI22_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9169955.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(4636..5940)
                     /locus_tag="IWI22_002631"
     CDS             complement(4636..5940)
                     /locus_tag="IWI22_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9169956.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(6129..>6591)
                     /locus_tag="IWI22_002632"
     CDS             complement(6129..>6591)
                     /locus_tag="IWI22_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9169957.1"
                     /translation="GDTIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEW
                     LQSVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKD
                     GLPKEMDFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         1763 a         1408 c         1200 g         2220 t
ORIGIN      
        1 ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg cactagaagc
       61 agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc gtcctttttt
      121 agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg ttaaattccc
      181 tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat cgtcagcttc
      241 tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc ctaatttttc
      301 ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga atgcagatcc
      361 gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga atactttgaa
      421 tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag ctgaaacccc
      481 tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat cactgagata
      541 ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac
      601 ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa tcgtctgtac
      661 attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag
      721 accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta cattgacgcc
      781 tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca tcaaaggcgt
      841 cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt aatcatctaa
      901 aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt ctcgacgata
      961 gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta gttcgaatcc
     1021 tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg tcttagttgc
     1081 ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc
     1141 atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc cagcagtata
     1201 cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt caatatcgtc
     1261 catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat aattcttaac
     1321 gatcagcaaa actccttttc cactatcggc ttccttaata gccgtctgaa tctgatctgg
     1381 agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt cccctacaaa
     1441 cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa cttgttccgc
     1501 tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt ctggatagct
     1561 ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca tgattttttt
     1621 caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt tgctgagtat
     1681 catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa ggcgcatcca
     1741 ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt tccgagttca
     1801 ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt gattcattga
     1861 ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg ccgagagaaa
     1921 aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata ccatctgtta
     1981 ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc ctttctttta
     2041 catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat ataataaatt
     2101 tttcgtttgt caaattaagc tagacaaatc atctttacct acgtaactca aaaatacttc
     2161 caatggtgtg cgatagtgta atgattttct aggaatatga tttcgttttg atgccaccga
     2221 ttggataaag gtttcggata ccttattaaa atccatagct ttatataagc catctttacg
     2281 taataagcca ttcgaatgtt catttagtcc tcgttgagaa ggtgttcctg gatcagcgaa
     2341 ataaatcgaa atatcattgg tattactaat ctttttccaa ttcgagaact cttttccaca
     2401 atcaaatgtg atggatttaa acaagtgttt cggtacagat tgaaaccatt gattcagtct
     2461 attctcaata tctatagctt gcctaccagc gggtttcaag gtgatgatca ctttagataa
     2521 tcgctcaact aacgtgatca ccgcactttt atgttttcca ccgacaatgg tgtcaccttc
     2581 taggtgacca aactcatgat tgaactgtat ataatcagtt gttctatcct gaatcgaacg
     2641 gcgaaaagct tgttttcctc ttttttcttt atgtccgttt ggttttcttt tccctttcat
     2701 tggtaaatcg tgagggtcaa aggttttatc tttaaacatc cgatatagtg tacgtaccga
     2761 acaagcaata ggaaactctg ctcgaccaat aaggacatct ggagtccacc cttgagccac
     2821 ctttttttga atgtattctt gttgcccatc tggtaacaca atcggacgtc ttccgcagtt
     2881 acgtttgttt ttcttatatt gttcatagta gcttattgcg cttccaccag atttaagaaa
     2941 acgatagaca ttataaatgg tttgtcgaga acgttgtacc agatgggcta cttttgaaac
     3001 agagtgtgat tgattgaaat aagcttctat cattacgagt tcagttggtg taagatgggt
     3061 ataggtcatt tgtactcact ccttataatt ctttcgacgg aactattttg agtgtagcac
     3121 aaatgacttt tttagttgtc tagcttaatt ttacaatcgg cgatttctaa ttttgggaaa
     3181 atatgtgtag tgcttcttga agtttgttat actttaatta aaagagagga gtgatcgaat
     3241 gattacaaca acaacgaaca gtgtagaggg aaaagccgta gtagcttata aagggatcgt
     3301 ttttggtgag gtcattactg ggatccatgc atttaaagac ttggaggcgg ggttgcgaaa
     3361 tatctttggc ggccgctcaa aaagctatga aaacgaacta atgggtgcaa gagaagaagc
     3421 acttgccgaa atggccgaac gtgcaaaagc attaggagct gatgcgatca ttggcgtaaa
     3481 aatggactat gaagtattag gcagcgacaa tggcatgctg atggtcacat gtagtggaac
     3541 agctgtctcc acgagataag taggggacat ctgttttaat aactaaagag agatgggaca
     3601 tgtagtggca ctcaagaatt agatttttca gtctaacttc gaggtactct accttcccat
     3661 cctctctttt ttatttcttt ttgcggatga ttccgtaagc tgctgcgact aacacacccg
     3721 cccctgctaa ggaataactg acagaagctg ttttttcatt tgtttttggc aaagtctgac
     3781 ttgttgattt ggatggctct gtcgtttgct gaactgtttc tgtcgtgctt ccagaggtca
     3841 ctgatttttc tcctgccgaa tgcaaaatat cttttattcg ttggaaacta gctgctaagt
     3901 cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc ttcgctgatc tttccgtgga
     3961 ttgcttgact ggccgctgtg atatccgctt gagtttgact tagctttcca gaagctgttt
     4021 gagcaacctg atttaattgc tgattgaatt cttttgcttg ttggatcttc ccgtcaagat
     4081 ctgtttggac agcttccgct tttgctttcg cttcggcaag ctttttctca agttgtgcac
     4141 ggtcatttcc cactttattt ttaagatcag ctaaatcttg atcgattgct gcttggtctt
     4201 ctttcaattt gttttttgca gcttctaatg cttcttcgag ttcttttctt ttattttcca
     4261 agtcttgcaa atgatcgatt tggtcagaag aggtagaact atttattgat gtatcttctg
     4321 aagatgaaat cgtttctcct gaattatctg tcggtacatt ggaagtcatt tctgtttccg
     4381 aggcaccgtt ttcagcacca gaagagctta tggttgttga aggttctgtt tgagcaggtt
     4441 cagaagtttc cgcaaaagct gtctgtccca ataagaaaat actggaaaca gcaattggcc
     4501 agattaacgg cttcattctt tttttcatca cttttcccat ccttactact ttttaactaa
     4561 tttcttatta taagcaaaaa tgtttggaag aaccataaac acaacaggct gtttgtctaa
     4621 aaaacggtgc atcttttatt tttttgtacg ttccgggctt ttccatgtcc cgcttccata
     4681 acctaaaatc gttttgactg ctggaatgaa agtcgtcacg atcgttatcg cgttcactat
     4741 ccagaacaaa agcatatata gtggtgcgaa aagaaggtat ttcaattttc ttcgtcgatc
     4801 atctacaatc agcgaagcta gcaactggaa aaatcctgcg accatttcaa agcagatgaa
     4861 cacaaaactg aatgtaaaag taatataaat ctgttcgtag tttccttgat atacataatg
     4921 cccgattaat ccagcaaaaa gaactacgga aatacaaaag aatattgacc agacaatact
     4981 caatgtttga tcaatgaaga tgatcgttct tccaatatgt ttaaaaggat ggagcatcac
     5041 ttttttgaaa ttcgtcagcc agacttctgt tccacctttt gcccaacgtt ttctgtgtcg
     5101 atatagcatc ttcaacgtca caggtacttc catgaaaaag ataatctcag gagcaaaaac
     5161 agataaccag tcatttaact gatgatccca agcaatactg atgtcttctg tcgctcgatc
     5221 ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga tacatcgtat ttgctccact
     5281 gtacgcataa agaccaccga aaacagctga ctgtgttcgt ttaatgatgc ccacgatact
     5341 tgagaattca accgtttgag attttgcaat caatttggta cgattttgga catccatatt
     5401 tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg aaataattca catacctaat
     5461 cagtgcatct ggctccggta ccgtgtctgc atcattgctt aaaatcaatt tcccttttgc
     5521 aaaggcaagt ccgatattaa acgcgtgggc ttttccttta tttttttcga tccgaaccac
     5581 acgaagatta gcgtattttt tcatcagtct agctaatatt tctggtgttt ggtccgttga
     5641 accatcgtct gtcactaata cctcatagtt gtgataattc aatttcgtca tcaaatactc
     5701 gattgtatct tcgatcacaa tttcttcatt atgggctggc accatgatcg tgatgaacgg
     5761 ttcgacactc aagggaacat ctacccattc tttttgctta tgtttgtata aaaacacata
     5821 acaccagaca ccaataaacc atacaaatcc gcctaaaatc ggataagaaa cgagaaaaaa
     5881 atacataata gacaaacaag tatctaagaa taaattacct gtatgggtga ttgagatcat
     5941 ttcagttcca ccccattttc tttatacaaa tctgcaatga atgtcgtatc tagatttttc
     6001 tcttcggaga cagaataaaa acggaccgtt tctctctctt cttttgttcc aaaacgttcc
     6061 gtatacactt cttcaagtaa ttcttgtctt tttttcaatt tttcatcttc atattattat
     6121 ttgtcaaatt aagcgagaca aaactttcca tttacgtaac tcaaaaaaac ttctaatggt
     6181 gtttggtaat ttagtgattt tctagggata tggtttcttt tggacgcaac ggatgagatg
     6241 aatccttgat tgacttggtt gaagtccatt tcttttggta gtccatcttt tcggaggagt
     6301 ccgtttgaat gttcatttaa tccccgttgg gaaggcgttc ctgggtctgc gaaataaata
     6361 tcaatatcat tcgtattact aatacttttc caattagaga attcttttcc acaatcaaag
     6421 gtaattgatt tgaaaagatt tttgggtacg gattgaagcc attcattaat cgaattttca
     6481 atatcaactg ccttacggcc ttctggtttc aaggcgataa tggcttttga aagtcgctca
     6541 acgagcgtga tgacggcact tttatggtgg atgccgacaa tcgtatcacc c
//
LOCUS       DACDNO010000103         6532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDNO010000103 DACDNO010000000
VERSION     DACDNO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.103"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(142..1086)
                     /locus_tag="IWI22_002633"
     CDS             complement(142..1086)
                     /locus_tag="IWI22_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9169958.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1220..2611)
                     /locus_tag="IWI22_002634"
     CDS             complement(1220..2611)
                     /locus_tag="IWI22_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAP9169959.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHNKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2737..3147
                     /gene="tagD"
                     /locus_tag="IWI22_002635"
     CDS             2737..3147
                     /gene="tagD"
                     /locus_tag="IWI22_002635"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP9169960.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3323..3736
                     /locus_tag="IWI22_002636"
     CDS             3323..3736
                     /locus_tag="IWI22_002636"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9169961.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3893..4150
                     /locus_tag="IWI22_002637"
     CDS             3893..4150
                     /locus_tag="IWI22_002637"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9169962.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4237..5475
                     /locus_tag="IWI22_002638"
     CDS             4237..5475
                     /locus_tag="IWI22_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9169963.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            5465..6205
                     /locus_tag="IWI22_002639"
     CDS             5465..6205
                     /locus_tag="IWI22_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9169964.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
BASE COUNT         2220 a         1226 c         1100 g         1986 t
ORIGIN      
        1 aagttctctt tttcatgtaa aatatttaat tatatatttc ctaaataaaa agtaaaacaa
       61 gaattaaata gttggttata caactttaat tcttgtttta ctttttagaa ttatttatcc
      121 aaagatgcaa tcattttttc atcaaatttc tgtctttaaa taccgttcca ttccgctcaa
      181 tatatcaaat ttaggctgat agcctaaaga acgaattttt gaaatatccg ctaaggaatc
      241 acgaatatcc cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg
      301 atcaattgaa tggatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg
      361 ctgtcctaaa gctttttctt ccttggcaac aagtaaaagt gcttgtataa catcttctat
      421 ataaacaaag tctctagact gactcccatc gccaaataaa gtaaattccg ctttttctcc
      481 tgccaattgt tttttgtaac gatccactag aatcgaaatg actccgctat atggagagtt
      541 tggattttga tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata
      601 taaatgacag taatccaaaa catattgttc tgctgcaaat ttatcgatag catatggtgt
      661 taatggacgg atgactgatt cttctttttt tggcagtgtt ggttcatcac cataaactgc
      721 agctgatgaa ctaaatacta atcgttttaa attagattga tatttccgtg ctaactcaag
      781 caataaaagc gcactgtcaa aatttacacg atgtgtttct acaggccgtt ctacagaatc
      841 tgccacacta gctacagcag ctagatgaaa aatataggca aactgatatt ctttcatgat
      901 tctttccatc aattgagaat ctgatacatc cccctcaatg aaagtcactt gtttatccat
      961 atccaagttt tctttttttc ccattgataa atcgtcaact actacgattt tgttttcttt
     1021 tcccaaataa ttcgctagcg ttgaaccgat aaaaccagct ccgccagtaa tcaatatagt
     1081 gtccatcaaa ttcactccaa ttgcacattt tcgtatcaaa taatattagt ttaccatttt
     1141 tatattgtaa aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt
     1201 catataccta aaaatagttt caattagtta ctccttcttc tttagatata gttttttgga
     1261 tagaacccag atatccccaa aaaataaatg aacctatcgt actaaccaaa atgagttctg
     1321 tgataaataa tgccgaaact gctattgtaa gaatcattaa aatagcaaaa aagtgcttat
     1381 cttttactag atttggttgt gtgaaacaat aacgcaaggc tttaaagcat tggataaata
     1441 agaaggttaa caatgtcaat agacctaatc ctcctgtagc agccatcact aagaaaataa
     1501 aattatgagc agttaaagta tcttttccca ttaatgtata tggtagttta ttatgtgcat
     1561 attcaatata gtagcctgga gaacctccgg atatcggctt actcttgaat atttcaattg
     1621 cacttttcca aagttcaaac cttaaattag aaacatcccc gctatcagtg tcagggcgtt
     1681 ttaaatcaac tttattgctg ctagttgttc ctgatgaatc agatgtatct ttttttactt
     1741 ctgtaatatg cggaacatgt gcccatgcat gagcagttat atctgttaca taatacatcg
     1801 caattatact tagcaatcct actactaatg cagttagcca tctttttaaa gtattagttt
     1861 taagaaaacg actactataa cttgtaaaga aactaaaaac aaaagttgtt attaataaac
     1921 aaactaatgt ggtgcgtgaa cccgataaag caataaacga gaattgaata aaaatattaa
     1981 ttacaaaaaa tattttaaat agaagattta aatgcttgta agtgaccaaa taatatatag
     2041 caataatgat gacgattaat gatataaccg caccaaaatt tggatctgta tatacaccaa
     2101 ataaacggtt gcctaaccac cctaaacgaa ctgggtaata cttatatttt attggaattt
     2161 ttatgccata ttgaatgaca aaccagtata atgatactaa attagctaaa aaccatacca
     2221 atgaaagtat ccaggtaaat actttgaaaa aaaagttact actatagtct ttgccaaact
     2281 gataaatcat aaagaaaaag attaattgcc acatgattaa cttaatattt tcgctatatc
     2341 catattttaa attcattaaa ctgcttacta gcatcgcgcc aataaaagca attaatagcc
     2401 aatcatattt ttttagattt ttttctctaa atgcaataat tgcatctata atgatcaaga
     2461 caactcccgc tattccagca ggccaaaaaa gtaaagtatc atatctagta ggtaatagta
     2521 aatacgggcc tattgctcgt aacattaaaa taaaaccaaa ataaataaaa taccaacctt
     2581 tcacatattt taaaaatctt tcattcaaca tttttcctcc atccacctat ttctcataaa
     2641 tatttcttat cctttttagg ataaactggt cctttcaatt ctaacaaaaa aatgctaaac
     2701 taacaatgta tattaatgaa tttcgagagg tgaaagatga aaagagtaat tacttacggt
     2761 acctttgatt tgcttcatta cgggcatatc aatttactga aacgagcaaa acaatacgga
     2821 gactatctta ttgttgccct ttctacagat gaattcaatt ggaatgcgaa acaaaaaaaa
     2881 tgttatttta gttatgaaaa aagaaaacag ttacttgaag ctattcgcta cgttgattta
     2941 gttattcctg aagaaaattg ggaacaaaaa gtaaaagata taaaagaata ccatgcagat
     3001 tatttcgtga tgggcgatga ttgggctgga cattttgatg aacttgaaca actaacagat
     3061 gcacaagtga tttatttacc aagaacacct gagatctcta caactcaaat aaagaacgaa
     3121 cttaattctt taaatagtga taaataatat tttttaaaca ataaaagaac aatatatctg
     3181 gataagacgt aaaaaaatgg ctctataatt tctaggactg atagaatttt ctgtcggtcc
     3241 tagatttttt gtaaaaaaaa acagacataa aaaaattccg atagaataga gtcgtctaaa
     3301 acatcaatct gaaaggaagt ttttgatatc catttctcat tctatcaaag agctcttcac
     3361 tattaaagat aaaaatattg aaattaattc ttttcaagaa actttttata aggacaagaa
     3421 agccatcgta atcgaaggtc cattagttcg cacttttaga cattgtctat tttgtaaaga
     3481 atctgcaaag cagatcgaga aaaacggcaa gaagatttca atgattatct taaatcgcag
     3541 tgggaataaa caaacctatt tgcgtcttag aaaacaacat taccactgcc aatcatgcgg
     3601 gaaatatttt actgctcata cgtatcttgt cgctccttca tgttttattt cgaaacaagt
     3661 tcattacacg atattagaag agttgaccga gcgtcaagca ttaaagacaa ttgcgaaacg
     3721 ttgtgatttt cagtaacgac agttcaacgg acattatctg ctctatcggc aacgctgcga
     3781 cagaagctag actatttacc tcattgttta ctattcgacg agtttcgctc tctttcaatt
     3841 ttccatggga agttctcctt ttcttgtatg aattgagaga cagggaagct ctttgacatt
     3901 ttgtccagcc ggagaaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc
     3961 tcatatgggg gttcgataca ttgtcacaga tatgaatgcc ccttattttt cattagtcaa
     4021 agaacccttc ccaaatgcac aaatcatcat tgattgcttt cacattgggc aacacttgaa
     4081 caagtttttc gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aatgtcaacg
     4141 gcacctttaa aatgtaggaa aacggcaatt taaaattgtt ggtttaccta catttttgta
     4201 tgctcttctt atcccaaatg aaatggaggc tcagggatga gaaaagacat acgcgaagga
     4261 gtgatgatct acgtgatcaa tgaaatcaaa cccaattatg ctgcactagc aaaacaatac
     4321 gactgcgact accgaacagt taagcatgct tacgaagaag cccaagttaa agaatcaaaa
     4381 cctccagaac gtaagaagcg tccaagtaag ttagatccct acagagaaat catccaagac
     4441 aaaatcaaag accaatgcag tgcttacagc attttcaagt ttattgagca caaaggattt
     4501 ggaggaagct acagtttagt aaagacctat tgtcgccaga ttaagcaggc gaaagtacac
     4561 aaggcaaccg tccgagtcga acatagccct gggctttctg ctcaggttga ctggaaggaa
     4621 gatatgcgac taatcagtaa tcagggcgaa atttttgagt tcaatatctt tttgtacgtc
     4681 ttgccatatt ccaagaaaaa atatgtcaca ctaactttcg accgaaaaca agacacactc
     4741 tttgagtgct tagatgatgc gttttatcaa accggtggcg ttcccaaaga aatctggttc
     4801 gataatatga agacagtcgt tgaccattct cgtagtcagt tttccaaacc gcatttcaac
     4861 aaacgttttt atgctttcag taaggatgcc ggcttcacac caattgcctg tcggccattt
     4921 cgtccgcaaa caaagggctg tgtcgaagct ttagccagaa ctgttgaccg cctacgggtc
     4981 tataatcatg aattttatga tgccgttgag ctgtgtcatt tagtgaataa cctttgtgtt
     5041 gagttaaata acgaggtttc tcaatcaact gatcaaacac cagatgaacg ttggcagacg
     5101 aacgaaaaag agcacctgca tccataccaa aaaaaccttt tgaatcccta ttttgaagac
     5161 tttattcgcc gtaaagtcac caaggaagaa atggtcattt tccgtaaatg caggtactct
     5221 gtggcgcctc gctatatcgg taaagaagtc gaaatcgaac tctccgataa agaagaccaa
     5281 gtacaaatct attataacgg agaactgatt cggtcgcatc cattaacgac aaaacagctg
     5341 aactaccacg aggaagacta tttccaaatc ttgaaatccg atgtcatgag tcacaaagaa
     5401 gacgatggaa tcagagcgta tactcgtgaa agtctcaagc aatatgatct ggtggaggtt
     5461 gccgatgaag aatagccatt accaagaact gatgaccaac ttagagactc ttggtttgaa
     5521 tcatatgcgg gaatatgttc ctaactacat cgaagtggcc aatcgagagg agctttcttt
     5581 aacggaagct cttctcgagc tgacacgcag agaagttgat catcaaaata agcaaaaaat
     5641 gaatcgtgtg gtggcacgcg cccgcttttc caagagaacc agcctggaag agtttgattt
     5701 tgccttccag ccaagtatca ataagcgcga agtgctagac ttaaggcaca tgggctttgt
     5761 ggaaaaacat gagaacttgg tttttatcgg cagtcctggt gtaggcaaga cacatctgac
     5821 gattgcattg ggaatcgaag cctgtcgtca aggctatcgg acactattca tcacctgtca
     5881 tgagctgctt ttgcgtctgc gaacagccta tgaaaaaggt accctagagc gtgttctcaa
     5941 acgatacgct cgctatgacg tcttgattat tgatgagatt ggctatcttc cgattcaaaa
     6001 acaggaagct gatttattct ttcagcttct tgccatgcgc tatgaacacc gctctacgat
     6061 cattacgacg aacattgtct tatctcgttg gggggagttg ttccaaagtt ctgagatcgc
     6121 agctgccatc cttgaccgtt tagtacatca cgtcaaggtg ttcaagatca caggcaagtc
     6181 ttatcgtatg agagacaaga tctaaagtgt cgattgtcga caattactat tgccgaaaac
     6241 ctacattttt atcctgccca aaacctacaa ttttaagctg cccttgacat caaaaagaca
     6301 cttcacaagc taagtgctat cgacaattga aacctttgta taaattactt ctataatcag
     6361 atgacgagtt ggattataca acattcaaga aatggcagaa ttttcaatgg cgctatttaa
     6421 cagaaagtga agtagttgat tgtctaccct caatctctta tgaacttaaa atggcttagc
     6481 agtattatca aaagctgctt aacaattatc acaagaaaca gctagctact tc
//
LOCUS       DACDNO010000104         6456 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDNO010000104 DACDNO010000000
VERSION     DACDNO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.104"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1138..1536)
                     /locus_tag="IWI22_002640"
     CDS             complement(1138..1536)
                     /locus_tag="IWI22_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169965.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKYT
                     SMKPCILDWQWLIDAVLDVKCSKYNITLGC"
     gene            1752..2807
                     /locus_tag="IWI22_002641"
     CDS             1752..2807
                     /locus_tag="IWI22_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP9169966.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2827..3171
                     /locus_tag="IWI22_002642"
     CDS             2827..3171
                     /locus_tag="IWI22_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169967.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDSKVAEVKNKPTDHNPIQQEHPSTVTSGHSAKKEKPML
                     KGFNAPSNKDLL"
     gene            complement(3352..3648)
                     /locus_tag="IWI22_002643"
     CDS             complement(3352..3648)
                     /locus_tag="IWI22_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169968.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETRSSLVDK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3652..4470)
                     /locus_tag="IWI22_002644"
     CDS             complement(3652..4470)
                     /locus_tag="IWI22_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9169969.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4690..4911
                     /locus_tag="IWI22_002645"
     CDS             4690..4911
                     /locus_tag="IWI22_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP9169970.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4913..5179
                     /locus_tag="IWI22_002646"
     CDS             4913..5179
                     /locus_tag="IWI22_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9169971.1"
                     /translation="MSYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5292..5831
                     /locus_tag="IWI22_002647"
     CDS             5292..5831
                     /locus_tag="IWI22_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169972.1"
                     /translation="MYNKSVNKLINLQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
BASE COUNT         2258 a         1078 c          967 g         2153 t
ORIGIN      
        1 taagtttctt tatattacta cgcgccttgc gtataaaact tctttattca tcccacgatc
       61 catgttattt tatatttcct ttttcatgtt atttttagcc agaaaccgat caagttttta
      121 cctattccca aaatccattt attcgttatt tttttctgat taagaataat tatttgatgc
      181 ttaaattagc taaaaaagcg atttgttact gatataatca tgatattaaa gctgaattag
      241 gcagaaagat aaaatgttcg caaaatcaaa gtatcaatta tcttgttttt ttataaattt
      301 gtaagtaaaa atctaaaaag ttttctttta attggtatat gtattaaaat cacattcaat
      361 cactcataaa agtatgcgat ttcatttttt tggatgagat taatatcaat aaataacgat
      421 gtaaaaacca tttattatgc tcttttataa gggtattaat tgatctaatt tgaaaaaata
      481 attcacaatt taaatgtttt aatattacga ataaaataat gttatcgtaa aatggtataa
      541 aaaagctaaa tattatattt ttgttatttt attagcagaa aaatttaaat agttttacaa
      601 gaatccgtag aaatatattg gttttgcctt caaattaata ctggatcata ttaatctgaa
      661 tataatatat gaactaataa atagcttaac ctagctattt tgttttttct gatcactatt
      721 tatacagcat tagtaaataa aattttaaaa ataaaaaaaa taaggcaaaa aaagtatcaa
      781 cataaacata tacatataca cgtttatgtt gatacactta atttgtcctt taaaaagcac
      841 ttttttgata ttatgttatt ttataatctt attacaagct gattgttagg cgttgccctt
      901 tcattacatt tattaacggt tattttatcg tttaatttat taattatttt tgatctaaaa
      961 cttagcttaa caaataaata attaaccaac cgccagataa aaatgcgatc attgctaata
     1021 cataagcaaa gataaaaata tgttcgatca gcttaaaccc tgatgcgatt aagcagacca
     1081 agtaaaatag aaattctctt acagataatg gtaattttct aactagcata ctcataatta
     1141 acaccccaaa gtaatattgt attttgaaca ttttacatcc aatacagcat caatcaacca
     1201 ttgccaatcc aaaatacaag gcttcattga agtatatttt tcagatgaaa agatataata
     1261 taatttcttc ggaataatat tttcagttgt tgggatcaag ctaaatctaa taccgtctaa
     1321 ctcatagcta ccagctttct ctccattaaa aatttcctta gcactttctt ctaaagaatc
     1381 aaaatatttt tcgacagcaa cctttgaagc ttttactatt gaagcatttc ccttttcgat
     1441 aaaatctgct tttgttttac cgacaataag taattcttga tcagtaaaat cttctaaaat
     1501 aatggtagtg taattcttat ttcttctcaa tcgcatcagg atcactcctt aatataattt
     1561 atgtttttat aatatcataa taagataaat ttgcaagcat ttatttacac ctttttattt
     1621 acactttatt ataattaatt atacttttat gattgcattt ttttcttata aacagttgca
     1681 actaatagta aataggtgta aactattttc gtaccgaaat accataaaca caaacagaaa
     1741 gaggtcatac catgaaagaa ccattattgt tttccgttga cttagggaat aaatcagtaa
     1801 aagaaatgag tacacacgtt tctccactct cacttccctc tcgtattttg aacaaaaata
     1861 tcattaattc aggacgtttt tctaatatag gttcctcatt aaaacgatca ctaaacgttt
     1921 ctgaatatag cctagtaaac actactgaaa gctattactt tgggaaggat atacacgagc
     1981 taggaaaaga tgactacgtg atagaatcac taggttttgg gatcagcaga tacaagcagg
     2041 aaacctacca aaatatccta tctttctctt tagctaacct agcacgttta gaagatcaat
     2101 ctgatgacgt tttaaccgtt aatctagttt taggtctgcc ttcatccgac tataccgaaa
     2161 caatcgtcaa atacgtgatg caacaagcca cacgacaaca ttcagttgaa gttgacggta
     2221 aggcttataa tataaacgta gataccgttt gggtactgcc acagccgatt ggaacactat
     2281 ataacctcct acttgctgat gatggtagtg tacaagatga aacattgatc agtcagaaga
     2341 tcggttttgt tgatattggc ggtggcacga aactttttga tgttgtccaa aattttgaaa
     2401 ttgacgaaaa caatcgtgaa caaaaggata ccggcatttt tactttatat aatcgtatcg
     2461 taaatatgct tgaccttccg atcagtccta atgctttcca acttgaaaag attatccgag
     2521 aaggcaaaga aaaaggcgta tatctattta agccaagcgg aaatgaaagt tttgatattt
     2581 cagaagtagt tgaaagagaa ataaaaacat acaccaatat ggttgttgca gaagttttag
     2641 ctacattcaa aaacttcaca accttagata aagttgtctt tactggtggt ggagcttcta
     2701 ttatcgacaa acaacttgta aaagataaaa ttccgcatac cttgtttgtt gaagacagtg
     2761 aattttctaa tgtaaaaggc ttttacaagt ttggtttaac tcaataaaac agcgaaggag
     2821 atcaacatga ataagggaaa atcagagcgt tatgtagtta taatcaatag agaaaaggtt
     2881 agcgagttac ctttagcaga gtttcttgaa tccgaatatg aaacactagg cgttaaatac
     2941 tctcagacga tcaaaaaagc tttgaaccta tatcggcgtg taaagaacaa tgaagttaca
     3001 attattgaag caggaaacga tagcaaagta gcagaagtaa agaataaacc aactgatcat
     3061 aaccctattc aacaagagca tccttcaacg gttacatccg gtcattcagc aaagaaagaa
     3121 aagccaatgc taaaagggtt caatgcacca agtaacaaag acttacttta aaaataaaaa
     3181 ggcgaggtta caatacctag cctttttatt atgtacaaat ataaacataa acatatacgt
     3241 atacatgtat ataaacatgt atacgtatat aaccataaac acgtatatga aatttagtta
     3301 agctatacct aaccgcttat tccaataaaa aaatgatcag ctttaaactg atcactcacc
     3361 aaaaacttca tcatacaagt tatctatcat ttcgccaagt aaagctgaat ctgatcgaat
     3421 tcctaacttt tcgctgattt tagatagttt ctttctttgc gatggttgaa gcgtgaattg
     3481 ataatttttc ttcttttcag aactatcact ttttagttta tctaccaaag aacttcttgt
     3541 ttctttaggt gtttgtttag gttcttcaac tgcttcaacc acttcaggaa cttcttctac
     3601 ttcagcttca gcgaattttt taaatctatt ttctttcact tcaatcactc cttattcttg
     3661 tgccgtatca attattttgt tgtatagcgt aaacgtgctt tcaaagaaat ccttattctt
     3721 acgatagata ttcttatcct tagcgaactc agcaatattc ttaccttcta agctagttgc
     3781 attaagcaat tccttctctt gtacatatcc aagataatta gataacgttg gtaccaattc
     3841 taggaactca cgacttgaat tagtattatg tttgatcctg ctaccgatcg tagcgtattt
     3901 agtttccatc agtgtttcac cagtcttaat atcaatcaac tgttttgcta gtgcatcaat
     3961 atctttacca agcttcagca aagcttcaaa accatcttta gatggatcag aaatgcctac
     4021 gacaagatca gatacagccc aagcattttg agctagtaga cctgcatcat tatgagtatc
     4081 aatcagaatt agatcatact tctctttaag ctgttcatag ttaacaacga tccatctaaa
     4141 cagtagagtt ctgttgttag gcatatcacg catttgcatc tcaacttgtt ttaaatcttc
     4201 acctgctact aaaatatcca tattctcatt tatagctaaa ggttcagctg atccatgaag
     4261 atataaatga ttgattgtat tctctctttt cgctttactg aaagcatcag gaaagcgttt
     4321 tgataggttt tgcgacttat cttcatcaat atacagcacc ttcataccta aattttcgac
     4381 agcatatcca cctagattgt agattgttgt tgttttacct gttccacctt tttcaatgtt
     4441 tccagtaatg attgacaact tcttttccat gattaaacct cccttattaa ccttttgtaa
     4501 ctccattata cactataaac acgtttatgt atatgtttat atacataaac gtgtttatat
     4561 ttatatttgc ttttatattc tacaacataa acatgtatat gtatatgttt atgtgtatgt
     4621 tcatatatat aactattgca tttgtcatac aatcgtagta caatgtaatt gaaaaggagt
     4681 tgataaacta tgccagtaat aagtattcgt gtatctaaag aagaaaaaga ttttcttgaa
     4741 aaagttgctg aattcaacgg agataacatt tctgaattta tgcgtagtaa ttccctgcaa
     4801 acagcagaaa cgttagtaga ttttgccacc tacaaggaac taatgaaaga gcatgaggaa
     4861 aacgatcaaa gtatttccca cgaagatatg ctaaaagagt taggcttata agatgagtta
     4921 ccaagtaaga tatgaacgtg aagctcaaaa agcgctaaaa aaaatggata agtttcaggc
     4981 aaaggttatc ctaaactgga tcgagaaaaa tcttgtaggc actgatgatc caagaaagca
     5041 tggtaaaggt ctaacagcta ataaatcagg ctactggcga tatagggtag gtgcataccg
     5101 tataatagct gatattagcg agcatgaagt gacaattctt atattgtcta ttggtcacag
     5161 aagtgaagtt tataagtaac agacaagctg ataaggcttg tctgtttttt tgttaaccca
     5221 acaaaatcac tgtttacgat cccactttac ctaaaaaaga acaatatcct cttgttatta
     5281 tacacattgg tgtgtataat aaaagtgtaa acaagctaat taacttacaa aaggagcgat
     5341 tttttatggg atggtatggt gcttattcat cagcaaatga agtgatcgaa gaaaagaaag
     5401 ggtacgccaa aaatggtttg gaaatcgtag ataccaaagt aacaaagacc tatggaatta
     5461 tccttttcaa agatgcagaa ggaacctacg atattgacag cttcatcttt cgagatgaaa
     5521 tgtacaaacc aatctacttc ttaaatgaca gtcctgctta ctttaagaaa attccgaaaa
     5581 aatggttgaa attctttgtc ggtgatcaag aaaaaatggt taagcgacaa ctagatagaa
     5641 ttaaagaaga aagcaataaa ccaaaacttg atgacttatt agttgcaggt aaaacttact
     5701 caatctggga tggatcgtat gtcgctgttt atcagtataa actcaaacgt agctttattt
     5761 ttcgactaca agatggatca ctcactcggt tcactcgact atcagcagga gatgttaagg
     5821 aagtagccta aaacttaaat acataaacat aaacgtgtat acgtttatgt ttatgtatat
     5881 gtatatgaat ataaacgtat taggtacgaa cgcttttaaa tgcttttctg ggctttgatt
     5941 ttagggtaga gctaaaacat caccatatga tccaaaacag ctcagaaacg catttaaacg
     6001 tatctgatac caatattata gaaggagtgt atcgtatgat taacctattt cttttggtga
     6061 cagctagtac attattcttt actggaatat cacttataat ttcctatttg atctataaga
     6121 aagccgaaac ttacacaagc aaagcaaccg gactagccgt ttcatccttt ttaactttgc
     6181 tgactttttt tgttactcta tcgttttctt ttgtttagca cagctgatcc atcagcaaaa
     6241 aacctctctt tacattatac acactagtgt gtataatgta attgtaaaca agctaattaa
     6301 cttttaggaa ggagtgacaa tatgctaaca gctacattac agcaacctac tcacgttgct
     6361 attgcagaaa gctatacaat taatgcaggg atcgttaaac agctgatcaa ggatcgagaa
     6421 ttcaaaaagt ttaacagtcg tcaagcgtat aacaga
//
LOCUS       DACDNO010000105         6432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDNO010000105 DACDNO010000000
VERSION     DACDNO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.105"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWI22_002648"
     CDS             complement(99..1229)
                     /locus_tag="IWI22_002648"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9169973.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWI22_002649"
     CDS             complement(1878..3080)
                     /locus_tag="IWI22_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9169974.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWI22_002650"
     CDS             complement(3140..3343)
                     /locus_tag="IWI22_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9169975.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWI22_002651"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWI22_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWI22_002652"
     CDS             4880..5692
                     /locus_tag="IWI22_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9169976.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1930 a         1313 c         1008 g         2181 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcataga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatattttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt ca
//
LOCUS       DACDNO010000106         6190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDNO010000106 DACDNO010000000
VERSION     DACDNO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.106"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            163..2760
                     /gene="adhE"
                     /locus_tag="IWI22_002653"
                     /gene_synonym="adhC"
     CDS             163..2760
                     /gene="adhE"
                     /locus_tag="IWI22_002653"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9169977.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2989..4095
                     /locus_tag="IWI22_002654"
     CDS             2989..4095
                     /locus_tag="IWI22_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9169978.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4112..5136
                     /locus_tag="IWI22_002655"
                     /pseudo
     CDS             4112..5136
                     /locus_tag="IWI22_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5142..5621
                     /locus_tag="IWI22_002656"
     CDS             5142..5621
                     /locus_tag="IWI22_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9169979.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5637..>6190
                     /locus_tag="IWI22_002657"
     CDS             5637..>6190
                     /locus_tag="IWI22_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP9169980.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT         2009 a         1086 c         1379 g         1716 t
ORIGIN      
        1 tttatatatt tttctataca aacacctgta aataaagcaa ttttatattt aaaaataact
       61 gtgaaaaaaa tcacaaaaaa gtgtttacat agaaaaataa atattgtatg atatacatgt
      121 gaattattta acaaataacc atgacccatt aggaggataa tgatggctaa agtaatagaa
      181 aaagaaaaaa cgaagacaat tgacgtacaa gcaatgattg atgatttggc agaaaaagca
      241 aatgcagcat tgaaagaaat ggaaagcttt gatcaagaaa aagtagacca tatcgtgcat
      301 gaaatggcaa tggcagcttt ggatcaacat atgccattag cgaagatggc tgtagaggaa
      361 acaggccgcg gtgtttacga agataaagca atcaaaaata tgtatgcgtc cgaatatatt
      421 tggcataata tcaaacatga taaaacagtt ggtgtgatca atgaagatgt ccaaaaaggg
      481 ttgattgaaa ttgctgaacc agtcggtgtg gtttgcggag taactccaac aacgaatccg
      541 acttccacaa cgattttcaa atcaatgatc gcgttgaaaa caagaaatcc aatcgttttt
      601 gctttccatc caagtgccca aaaatcttct gcagaagcag cacgagtggt acgtgatgct
      661 gctatcgctg ccggagctcc tgaaaattgt atccaatgga tcgaacatcc ttcgatcgaa
      721 gcgacttcta tgctaatgaa ccatccaggt atcgcgatcg tcttagcgac aggtggagct
      781 ggaatggtca agtctgctta ctctacagga aaaccagctt taggtgtagg tccaggtaac
      841 gtaccagctt atattgaaaa aacagcaaaa attaaacgtg cagtgaacga tttgatcgtt
      901 tcgaaaacat ttgataatgg aatgatttgt gcttctgaac aagctgtgat cgtggataaa
      961 gaaatctatg cagcagtaaa agcagaattc caagcacacc aagtttatat cgtaaaacca
     1021 gatgaattgc aaaaattaga agatgcagtg atgaacgaag gaaaatatgc ggtcaatcca
     1081 tcaatcgttg gacattctgc gatggaaatc gcaaaattgg caggaatcag tgtaccaaaa
     1141 ggaacgaaaa tgctgattgc tgaattggaa ggcgtaggcc cagattatcc gctatcaaga
     1201 gaaaaacttt ctccagttct tgccatgatc aaagctaaca acacggacca tgcgtttgat
     1261 ttgtgtgaag gtatgttgaa tcttggagga ttaggacata cagcagttat ccattcagaa
     1321 aacgaagagt tgcatgtcaa attcggtctt cgaatgaaag cttgtcgtat tttagtgaat
     1381 acaccatctg cagaaggcgg catcggtgat atctataacg aaatgattcc gtcattgact
     1441 ttgggatgcg gttcttatgg taagaactct gtttctcgta acgtctctgc tgtaaactta
     1501 atcaatgtca aaactgtagc gaaacggaga aataacatgc aatggttcaa attaccgcca
     1561 aaaatcttct ttgaaaagaa ctctctattg tacttagaga aaatggaaaa cgttgaacgt
     1621 gtcatgatcg tttgtgaccc tggtatggtc caattcggtt actgcgatac agttcgtgaa
     1681 gtattggcac gacgtaaaaa tgatgtgaag atcgaagtct tctcagatgt tgaaccgaac
     1741 ccatcgacaa ataccgtata cgcaggaacg aaactgatgg cagacttcaa gccagataca
     1801 gtaatcgctc ttggaggcgg ttctgcaatg gacgcagcta agggaatgtg gatgttctat
     1861 gaacacccag acacatcttt ctttggtgca aaacaaaaat tcttggatat ccgtaaacgg
     1921 acatataaga tcgacaaacc agaaaaaaca caattcgtat gtatcccgac aacatcaggg
     1981 actggttctg aagtaacgcc atttgcggtc atcactgaca gtgaaacaca tgtgaaatat
     2041 ccgttagcag attatgcatt gacgccagat gtcgcaatcg tcgatcctca attcgtcatg
     2101 tcagttccag catctgtcac agcagatacc gggatggatg tgttaacaca tgcaatcgaa
     2161 tcctatgttt cagtcatggc ttcagattat acacgtggat tgagcctaca agcaatcaag
     2221 ttagtctttg actatttaga aaaatcagta aaaacacctg atatggaatc acgtgaaaaa
     2281 atgcacaacg cttctacaat ggctggtatg gcgtttgcta acgcattcct aggtatttgt
     2341 cactcagtcg cacataaaat cggcggggaa tacgggatcc ctcatggacg gacaaatgcg
     2401 attcttttac cgcatattat ccgctataat gcaaaagatc cacaaaaaca tgcgatgttc
     2461 cctaaatatg actatttccg tgcagataca gattacgcag atatcgcaaa attcttaggt
     2521 ctgaaaggcg aaacaacaga ggaattagtc gaagctttag caacggctgt ttatgaatta
     2581 ggaaaatctg tcggcatcaa catgagtcta aaagctcaag gagtcacaca agaaacacta
     2641 gatacaaccg tagaccgtat ggctgaatta gcttacgaag accaatgtac gacagctaat
     2701 ccgaaagaac cattgatctc tgaattgaaa cagatcatca ttgatgctta caacggataa
     2761 gcaaaagaaa gcgtagaaaa aagataagaa aatagatgaa aaagaacttg gaaaacgttg
     2821 tttctccaag ttcttttttt aatttccaag taactgaaag ttacttttgt tatttaacat
     2881 ttaggtcaat tttagacagt ttttgttcaa aaagcgatat ttatagtgga cggtattact
     2941 gctatattaa ggatataaaa tgaaagcgtt taaaaaggtg gcgtaaagat gattattaaa
     3001 gaagagattt ttgtacctaa agaattagtg aaagaaaaaa ttgacctgct cgttgaaaac
     3061 ctgacaacga tcaaagacga taatggtgaa tttttgttgg actttgatgg actgaaagta
     3121 gatgacaaaa gctggacagt gtggaattgg ccacaaggtg tcggtctata tggtatttat
     3181 aaaaattacc agatgacaaa aaatcctcgc gcttatcaag tggtcaatga atggtttgag
     3241 gatcgtatgg aagaaggcgc tccgcccaaa aacgtcaata caatggcacc gctattgact
     3301 atggcttact tgtatgaaga cacgaaagat tcacgctatt tgccttattt agaacaatgg
     3361 gcagaatggg tcatgaatga catgcctcgg acaaatgaag acgggttaca gcatgccaca
     3421 tatggaccgg aaaataagaa tcagttgtgg gacgatacat tgatgatgac agtgcttcct
     3481 ttggcaaaaa tcgggaaact gttgaatcgc ccagaatatt tagaagaagc tcgttaccag
     3541 tttatgatcc atatcaaata cttgatggat aaacgaacag gattgtggta tcacggctgg
     3601 acgtttgaag gaaatcataa ctatgccgaa gcattttggg ctcgtggaaa ctgctggttg
     3661 acgattgcta ttccagagat cattgaaatc ttagaattat cgaaagaaga tgcattgcgc
     3721 aaattattga tcgaaacact tgaagcacaa gtgcgtgcat taaaaactta tcagtctgaa
     3781 agcggtttat ggcatacgtt gttggatgat ccttcttctt acgtagaatc ttctgcaact
     3841 gcaggatttg cttacggtat tttgaaagcg gtccataaac gttatttacc acaagaatat
     3901 aaagaagtcg cttatcgtgc tatccaagga ttgcttgaac aaatcgatga aaaaggagaa
     3961 gttcaaaacg tttcaatcgg gacaggaatg ggggacagtt tagattttta tcggaatatc
     4021 ggaatcacag caatgcctta tggacagtcg ttaacggtgc tttgtttgac agaattgctc
     4081 gtctcttatt gttaaaagaa aggaaaccca tatgaaaaaa acatcactgc tgtttgcagc
     4141 aagttcgctt agcctaggtc tgctaggtct aagcggctgc ggaaataatt ctagtgtggc
     4201 aactgtcgca gatgcagacg taaatgagat agttaccttg cgctttgctt atgcaagcaa
     4261 cagccagtaa ttgatgcaat gaatgaattt gggcgtttag taaaagaaaa aacagatggt
     4321 tccgtacaga tccagtattt cccagatgga caactgggtg gagaacgtga actgatcgaa
     4381 ttgacacagt caggagcagt agatttcaca aaagtaagtg cctcagcgtt ggaaagtttc
     4441 tctaaagatt attcgatttt ttccgttcct tatctattca atgatgaaga acatttcttt
     4501 aaggtgatgg ataatgaaga aataatggag ccaatctacc aatcgaccaa aaatttagga
     4561 tttaccggat tgacctatta tgattctggt cagcgcagct tttatatggt agacggtccg
     4621 atccatacgc cggaagattt gaagggaaag aagatccgtg tgatgcaaag cgaaaccgct
     4681 atcaaaatgg tagagctttt aggcggttcg cccgtaccta tgggaagtga tgaagtgtac
     4741 acgtctcttc aatccaattt gatcaacgga tcagaaaaca atgaatttgt cttgtataca
     4801 gctggacatg gcggggtagc taaatactat tcctatgatg agcatacacg tgtgccagat
     4861 atcatcatca tgaatgatgc catcaaagaa cagctaactg atgaacagga aaaagccatt
     4921 gaagaagcag cgaaagaatc aacggttttt gaaattgaag cttttgcaaa agcaattgaa
     4981 gaagaaaaag ctttagcaca agaagaacat ggagtccaat tcaatgatgt agataataca
     5041 ccgtttcgag aggcagttgc cccacttcat gatatattca aaaacgatcc tacctacaaa
     5101 gacctttatc aaaagatcca agagcaagga gcgtagctgt aatgaaaatg attttaaaca
     5161 aggtattaga gatcctggga gctgttttgc ttgttgggat ggtcgctgtt gttttatggc
     5221 aagtattttc cagaacggtt ttaggaaatc caaatacggt cacggaagag cttgttcgat
     5281 tcggtcttgt ctggtttgcc atgttgtctt ccgcgtatgt tgtgggacaa aaagcacgtt
     5341 tagccgtaac ggttttaagt gaaaaattaa ctggcaaaaa aagtaaaatt ttagaagttg
     5401 ttgttcaaag tttatttctg gtatttgctg cgatgatcat ggtgtatggc ggatggaatg
     5461 ctgtcacatt gacaatgggg cagatctcgc cttctttaag tattcctatg ggatatgttt
     5521 atctgtcagt acctgtatcg ggggtactca tcattatcta tagcctgatc aatctaatcg
     5581 atacattaag caacaagcca gcaattcaag aaaaaatata aaaatggagg aataaaatgg
     5641 caatagaagc cggattagtg ttattttttg tatttgttgt gttgcttgta ttagggatgc
     5701 cgattgctat aagtattgca ttctcatcga tgatgacttt attgttagtt ataccgtttg
     5761 atgtttctgc ttttagttca gctcaaaaaa tggtgggaag tatcaatagt ttttcgctga
     5821 tcgctattcc gtttttcgtt ctctcgggca ttatcatgaa taatggaggg atagctaaga
     5881 aactagtgga ttttgcgatg ttgttcgtcg ggcgcgtgcc gggggctttg gctcatacga
     5941 atgtgttagg gaatgcttta tttggttcca tctctagctc ggccattgcc gcttctaccg
     6001 cgattggtgg agtgttgatc cctcagcaag tggaagaagg atatgaccgg aagtttgcga
     6061 cagcggtcaa tatcgcgtca gctccgacag gtatggtcat tcctccaagt actgctttta
     6121 tcatgtattc acttgttgct ggtggtgctt cgatctctgc tttgtttatg ggaggttatc
     6181 tcgtaggcgc
//
LOCUS       DACDNO010000107         6185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDNO010000107 DACDNO010000000
VERSION     DACDNO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.107"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            52..1014
                     /locus_tag="IWI22_002658"
     CDS             52..1014
                     /locus_tag="IWI22_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9169981.1"
                     /translation="MDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKN
                     STANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFG
                     EAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQ
                     VPREYKVIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDS
                     IEVISLKDICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKN
                     HEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            1025..3229
                     /gene="topB"
                     /locus_tag="IWI22_002659"
     CDS             1025..3229
                     /gene="topB"
                     /locus_tag="IWI22_002659"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9169982.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            3475..4068
                     /locus_tag="IWI22_002660"
     CDS             3475..4068
                     /locus_tag="IWI22_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9169983.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            4084..4983
                     /locus_tag="IWI22_002661"
     CDS             4084..4983
                     /locus_tag="IWI22_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9169984.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            4986..5471
                     /gene="ssb"
                     /locus_tag="IWI22_002662"
     CDS             4986..5471
                     /gene="ssb"
                     /locus_tag="IWI22_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9169985.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5837..6097
                     /locus_tag="IWI22_002663"
     CDS             5837..6097
                     /locus_tag="IWI22_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169986.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2252 a         1060 c         1275 g         1598 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttggatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaaagaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagattggtg atcctaaaca gttaaaattt
      241 tttgagagta gtattgattt acttagctac gctgcactga atcgtgatca gttaaacgat
      301 acgtggttag tctctatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaga
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa aggtcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaactggt ttccgctcac
      661 aaagtcaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agtcattagt
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag attgtgaacg ctcgaatggt
      781 acgtatgatt ttgatcgttt ttatcaagga aaaggggata ttaattctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtgaaaa aggattggaa cgatcaattg aagcatgaga tccatcaaca ggaaattaga
      961 aagcaaaaac gtgcgatgtt gtttcaacaa ggcagacaac aagaaaggga gtgagatcgt
     1021 cacgatgaaa actgtcattt tagcagaaaa accgtcacaa gcgaaggcct atgccgatag
     1081 cttttctaaa gccacccgaa aagacgggta ttttgaaatc caagatcgat tgttttcggg
     1141 tgaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaacgtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tcgcttggac
     1381 gatcattcaa caagcaggcg ctgatcaagg aaaaaccttt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcaatta cgagacgcag aagagaccta
     1501 tcccaaattt gaagaagcac aagcacgcca gatcgctgat tggttgatcg gcatgaacgg
     1561 cagcccactg tatagtttac tcttgcagca aaagggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcagctg caggaaaaga ttagaaactt
     1681 ccaaaaagag ccttattttg aagggaaagc ccaagtaacc gctcaaaatg gcatatttga
     1741 tgcgaagctt gatcccaatg aaacccaagc gacgcaagaa gcatttgaag cctatttaaa
     1801 agaaaaaggt gttcaagtag gaaagcagcc aggcacgatt cttgaagtcg aaaccgagaa
     1861 aaaaagcgcg gcaagtccac gtcttttttc tctatccagc cttcagtcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tatccacgaa cagacacccc ttatattaca gaaggagagc atgcgtatct
     2041 gttggatcat ttagatgaat acaagcattt tttaaaggct gaatcattcc ctactcctat
     2101 tcatacaccg aatagtcggt atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaaa acagttatga cagaagcaac gtatgaaaag ctgacacctt tacaacaagc
     2221 gatttatgat caagttttaa agacgacggt ggccatgttt gcggaaaaat atacctatga
     2281 agaaactacg attttgacac aagtccagca gcttcagcta aaggcagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctatttgg caaagagagc gacagaaaag agaaggaaga
     2401 agaaccgcta cttcctaaag taacaaaagg agaaacggtg acggtagatc tacaaatctt
     2461 tgaaaaagaa accaagccac cacaacctta cacggaaggt acactaatca ccgccatgaa
     2521 gacagctggt aaaaccgtgg atagtgaaga agcacaatcc atattgaaag aagtggaggg
     2581 aattggtaca gaagcgacac gagcaaatat tattgaaacc ttgaagcaaa aggaatatat
     2641 caaggtagag aaaaataaac ttgtggtgac gaataaagga attttgcttt gtcaggctgt
     2701 tgaaaaagaa ccgctgttaa caagtgccga aatgaccgcc aaatgggaaa gctatcttac
     2761 aaagattggt gagcaaaaag ggacacaagc tacttttcta gcaaatatcc aaaaatttgt
     2821 ttcccacttg ttggaagtcg tcccaggaca aatccaagcc actgattttg gagcaacgtt
     2881 acaagaggtg aaagtagcca gtgaaaagca agaagcggcg cgtcatttag gatcttgtcc
     2941 aaagtgccag gaaaaagaag tcatacttta tcagaaggtg gcgacttgca cgtctgaagc
     3001 ctgtgatttt aaattgtggg taacgattgc caaaaagaaa ctcacagcga cacaactgaa
     3061 agagatcatt cagaacggcc gaacaagtca accagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggatttg agttttctga
     3181 aaagaagaag accacctaca aaagaaaagt gagacgtaaa acgagttaga attgacggag
     3241 ggaaaaacga tgagaaatac aacgcataaa gaacaagaaa tcgaaaatca aaaagccaaa
     3301 atacgttcag agcaggagaa aatgcgtgcc agcaaacgaa gactacagca attgaagaat
     3361 caacaacgga ttcaaaaaga acttgaatta gatcgataaa tcctcctgtt gttaatatta
     3421 aactaacatg ttatagttat gttagtttaa tattaacatt cgagagggaa agcgatgaaa
     3481 gataaagtca agtgcatttt tgagaaatac aatggtaatc tatccttgaa ggatgctaga
     3541 aatgaagggt tatcacctat gacgttagat cgtttagccg aaaaggacaa aatagaaaaa
     3601 atagcatcag gtttttatac cttaaaagat agttttgtgg atgaattata tctcgctcaa
     3661 agtatctata ctagaggaat cttctctcac gagacagcgc ttgatctttt tcaagtagga
     3721 acatatatcc ctaaaaaaat taatatgatg tttcctaaag gatacaatgt ttcgaaagag
     3781 aatttagatc agtatgccat tcaaccgcat tacacgaata aagaaaactt tgaactggga
     3841 atcacagagg cagaaagctt ttacggcaat aaaattttcg tctatgattt agagcgaacg
     3901 ttatgtgata tgtggaatcc cagatataaa gcaagcgtag aagtaaaaca agaagcatta
     3961 aaagaatata tgacaagtac cagcagaaat acaaataagc taagaagata catgaatatt
     4021 ttaaaatcac caaaagaaat gactttgtat atgttgccac tttattgaga ggagtaaagg
     4081 tgaatgacac cgacacaatt taaagcgaaa acaagaaatg ttgcagctga gaaacattta
     4141 aacccacaaa ttgttcaaag atattatatg atggaaaaat tattagaatg tattgttgaa
     4201 tcgccttatc atgatgattt tgtattaaaa gggggatttc tgatcggatc aaaatacggg
     4261 ttagaaaacc gttcaaccgt tgatattgat acgacttatc gaaattcgaa actaacaaaa
     4321 gaaaaattag aaactgtttt ttctgaatta acagctgctc ctacaaaaga tggcattcgg
     4381 tttgaattaa agggcttaac agagacgagg gaagccgatt attacccagg ttttcaagca
     4441 aagtttattg cttttcttga aaatgcacgt gtacctttta aattagatat tacttcaggt
     4501 gattctatta ttccagcagc attgatatat gaacataaat tgatgtttga agataaaaca
     4561 attcatattc cagcttatcc tacggaacaa attttagctg aaaaattaca tgccacgttt
     4621 agtttcggaa tagataattc acgtatgaaa gatttctatg atctgtatat gatccctaaa
     4681 ctagaacgta tcgatgagat aaatttgtat aagagtgttc aaggaacatt taaggaaaga
     4741 aataatcaat cagtgtttaa gaatttgtat aatcaggaaa tgcctcaatt gatgaagaat
     4801 gagggattga gggagaaatg gaaaagatat cagcaagaaa actattttgc caaagatatt
     4861 tctttcgaat ctaccttaac ttctgttgat tcgctgatgc aatcaattgt cgagcaagag
     4921 gaagtagaac gaaaacaaaa ttttaagaga tcacaacaaa tgaatcaaga aatggagaga
     4981 taaagatgat caataatgta actttagtag gtcgattaac caaagatccc gatttacgtt
     5041 acaccgcaag tggcactgca gtgaccactt ttaccttagc cgttaaccgt aattttacta
     5101 atcaaagtgg caatcgagaa gctgacttta tcaattgtgt gatttggcgc aaaccagctg
     5161 aaacgatagc gacattagct aaaaaaggca gcttgatcgg cgtcgtcgga cggattcaaa
     5221 cgcggactta tgacaaccag caaggacagc gtgtctatgt gactgaagtt gtggccgata
     5281 atttccaact attagaaagt aaagcagcga cagaaagccg tatgcacgct gatcaatcgt
     5341 ccagcagtcc aagccctacc acctttgaac aacgggatac agcgacgcct aacaacaatg
     5401 gattgaacgc ttcacagaat ccatttggtg gacaatcaat cgatattagc gatgatgatc
     5461 taccgttcta agtagcattt tctattggtt gcatttgcta cggtttatgt tataatttag
     5521 acaacaaaaa gaattttcaa tctataacca aaaaccacta gctggcgggc tagtggcttt
     5581 cttttacgtt tctattataa gaattaacgg gtaggcttac tgtctttctt ggcaaattta
     5641 caatcaagcc atttgcagat atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa
     5701 tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc agattaggcc ggggtagcag
     5761 acagcaagac tattatagca taattgaaat atccaaatga ttggattttt gctataattt
     5821 tagcgaggtg attgtgatga acaaatatgt tggtttgctt gataaaatcc gagtaataaa
     5881 aacgcaaccg ttattggttc gctttacttt acaaacgatt catgaatcga ttaattgtgt
     5941 agttgcagat attgaaatta tcgataaatt gctaattatg gatgatggaa aatacaatat
     6001 tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt attgctagca tgtatgttag
     6061 aaatcccgat cattttacgc gatcgatggg catataaata aaagaagagt tgtagtccta
     6121 aatgaggatt gcagctcttt ttgttttaca tagaaacagg aaaagacctt tttaggaaga
     6181 cacaa
//
LOCUS       DACDNO010000108         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDNO010000108 DACDNO010000000
VERSION     DACDNO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.108"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWI22_002664"
     CDS             725..811
                     /locus_tag="IWI22_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9169987.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWI22_002665"
     CDS             complement(932..1621)
                     /locus_tag="IWI22_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169988.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IWI22_002666"
     CDS             complement(1628..1906)
                     /locus_tag="IWI22_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169989.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IWI22_002667"
     CDS             complement(1866..2780)
                     /locus_tag="IWI22_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9169990.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWI22_002668"
     CDS             complement(2762..3142)
                     /locus_tag="IWI22_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9169991.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWI22_002669"
     CDS             complement(3592..3879)
                     /locus_tag="IWI22_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9169992.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWI22_002670"
     CDS             complement(3899..4123)
                     /locus_tag="IWI22_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9169993.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWI22_002671"
     CDS             complement(4763..5296)
                     /locus_tag="IWI22_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9169994.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWI22_002672"
     CDS             complement(5317..6159)
                     /locus_tag="IWI22_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9169995.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDNO010000109         6130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDNO010000109 DACDNO010000000
VERSION     DACDNO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.109"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(174..476)
                     /locus_tag="IWI22_002673"
     CDS             complement(174..476)
                     /locus_tag="IWI22_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9169996.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(696..1062)
                     /gene="ssrA"
                     /locus_tag="IWI22_002674"
     tmRNA           complement(696..1062)
                     /gene="ssrA"
                     /locus_tag="IWI22_002674"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1233..2006)
                     /locus_tag="IWI22_002675"
     CDS             complement(1233..2006)
                     /locus_tag="IWI22_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9169997.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2150..2500
                     /locus_tag="IWI22_002676"
     CDS             2150..2500
                     /locus_tag="IWI22_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9169998.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2481..3197
                     /locus_tag="IWI22_002677"
     CDS             2481..3197
                     /locus_tag="IWI22_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9169999.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3197..4645
                     /locus_tag="IWI22_002678"
     CDS             3197..4645
                     /locus_tag="IWI22_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9170000.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4715..5224)
                     /locus_tag="IWI22_002679"
     CDS             complement(4715..5224)
                     /locus_tag="IWI22_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170001.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5214..5939)
                     /locus_tag="IWI22_002680"
     CDS             complement(5214..5939)
                     /locus_tag="IWI22_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170002.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2091 a         1062 c         1182 g         1795 t
ORIGIN      
        1 acttataatt ggagtggtag ttaattagtg acttattttt gattttatag cactgatact
       61 ataaaatata gatatcatca tattacacaa tcttaatact aacttaaaaa atatcccttt
      121 tataagtatg gtgataaaat ccgttccggg ctaccttttt taggtagcct actttaatct
      181 ttataccttt ctggatcaac gaaagtatac ttcaaatagt cataacgccg atgatcgctc
      241 cgtgcgtctg gcacatcagt cacgatatca aacaaaaaat acacatcttt cttcattctg
      301 gtttttgttg ccggaatttt aaagtagttc ttattagagt aatagagatt gattaataag
      361 ctatcttcga ttgctaaaaa gaaaacttct gaatcccaca ccttataaaa atctttgaca
      421 aatctattcg aaggatcaaa tttaaaccat aattgtgttt ttccttccat caacataatg
      481 ttcacctcaa aaagagtata cgaactaatg ttctttttgt aaagtaggaa tatcattgat
      541 aatatggatg gaacatatat attaaaaagt gttctttttg aatgctattt tgaatgccat
      601 tttcattcat ttaacgaaaa tctgcgaaaa taaaaaatgc tcaaaccctt ataaaataag
      661 gatttaagca tctagcgaaa tctgctgaaa tctcacaatg gagacggcgg gagtcgaacc
      721 cgcgtccaaa cacatcgcca cttaaaaatc tacgttcata gtcaactcat ttaaggattc
      781 actttacagc ttgccgagtg acaggcattc tgtatcgcta gtctgatgtt ctctttttca
      841 acttacagac ggaaagttga aacgtatccc actaaagtta ggacccttac tcgagcacat
      901 gggcgatgcc gagaggatct acgctaactg tttttaggca gctaaagcgt aagagttgtt
      961 ttcgttttta gcagttatat ttaactgtaa cgtttttacg tagacgtaac ctacgaaacg
     1021 caattcaagc tcgactgtgc ctgtcgaatc cgtaacgtcc ccgttagcta acaagggtta
     1081 cgtgtatttt tcatacaaaa ttagtatagc atatattggg ataaattcaa taaaaaagcg
     1141 aatcatatcc cattatttat ataataactg cccctttctt aagaaaaaat aagcgatttt
     1201 cttaagaaaa gaggtagctt caaaaaaaga tcttaaatca gatcaaaata cttcaatgcg
     1261 tggacaattc cgccttctgt atttttcttt gtgataaagg tagaaagttc ttttaatccg
     1321 tcataggcgt tgcccatagc gattttgttg tcgcaagctt ccaacaaggc aaaatcattc
     1381 ggaccatcgc caaatccaaa agtaggaact ttttccagct gaagttcttt ttgcagacgt
     1441 ttgacacctg ccccttttga gattcctttc ttcactgtat cgatcgagta aggaccattc
     1501 cgataaaaag ttagctcagg aaaatgttcg tgatagtatt cgtcacctga ttgagataag
     1561 atcagcaaca tatttactgt gttttcttta aagcctaaag gatcaatttc cggaacatct
     1621 gaatgaatga agtcgtaagc attgatcatt gtttggctat gaccggtaca ccagattttc
     1681 tgatcgttat agtaagaaag ttcatgaccg ttcaactgta catgatcata cattttttga
     1741 cattcttctg aagaaaatgt ttgagaatag atttcttcgc cgtctactcg aatgaaagaa
     1801 ccattcatgg tgaccgcaga agtaatgccc gcatcttcca tgatagagtg gatttctgct
     1861 tctgtacgtc ctgtagcgat gacggggaga acattgtttt cttttaatgc gttcatagct
     1921 tttgccactt cgggtgtgat ttttgatttc gcatctagta atgttccatc aagatcaaag
     1981 aaagtgattg cacgataatt tgacacaaat aaaactcctt ttttattgga ataagtttat
     2041 cttaacaaag aatgaacaaa agacaaacct ttcgtgaaga attcctgaac atattggttc
     2101 agtcaacaat ttagagtata ctataagaag agaatttagg agggaaagaa tgattttttt
     2161 cagtattggt atcttattct taataattgg cagtcttttt cggattctgc cctcaaaagg
     2221 aaatctgcct ttttatgggt atcattcgcc tcttgccgca aaaacagacg ctcattggcg
     2281 gctggctcaa aagacaagcg gaaactggtt ttttcttatg ggcttactga tggcattgat
     2341 cggctattat ttaaagacat ctgggcatac gaattatttt ctgattgaaa tgcttctgct
     2401 agtatttcca attatgccta tttttattat gacagaaaag aaattacaga aatatgacct
     2461 ggaaactgga gggaacgata atgaatattt taatgattga agataatcaa tctgtttctg
     2521 aaatgatgca gatgtttttc ttaaatgaag gttgggaagc aactttcaag tatgatggaa
     2581 aagaagggct cgatgccttt ttagaaaacc cacaacattg ggatatgatt acactagacc
     2641 tgaatttgcc cacactggac ggtatgctag taggaagaga gatccgcaaa gtttcaagta
     2701 ctgtgccgat tatcatgctg acagctagag attctgaaag cgatcaggtg atcggcttag
     2761 agatgggggc agatgattat gtcacaaaac ctttcagtcc tctgacgctt attgctcgaa
     2821 tgaaagcttt gcatcgccgt agtgagttgg cagaagcaag agaagaagca gtagtttcag
     2881 atgatcattt tgatatcgag actagtcatt tcaagatgaa tacgaaaaca cgagaagcgt
     2941 atctggacaa ccggccaatt gaaggactga cgcctaaaga gttcgatcta ttgtttactt
     3001 tagcaaaaaa accacgtcaa gtattctctc gggagcagtt gcttgaatta gtctgggatt
     3061 atcagtactt tggtgatgaa cggacagtgg acgcccatat caagaaatta cgacaaaaaa
     3121 tcgaaaaggt cggaccacaa gtcatccaaa ctgtttgggg agtcggctac aaattcgatg
     3181 attctggtgt tgcctaatgc gttatttgta tcagcaactc ttagctttct gcggaatggt
     3241 cgcgttgatc ttattgatcg ttggagtttc ttttacacaa ctgacaaaac aaacgattga
     3301 agaaaacaat taccagcaac tgtttggata tgctgaatca gttgaaaaga caacgcaaac
     3361 ctatgccgat acgctcccgc agtttagtca agatcaggcg tttcaaaatg cattgctgct
     3421 gacagagcaa gtactgacag aacaaaatgt gaactttata tttatcgata aaaacgaacg
     3481 tgtcatttat ccgactaata cagctttact gaattttacc ttaacagctg atcaatggaa
     3541 aaatctgagg aatggacgtc aagaaaaatt cacatcgaat aaaaatattt taggacaagc
     3601 acaggcgacc tcttacgcac ttgtgccatt taatttgaat catgaattct atggagcact
     3661 tgtagttagt caacctgcgc gaaatatcga taacagcgtt cgtccaatta cactgaacct
     3721 gttcaaagga tttatttttt ccagtatcgt tgcagttatt gccagctatg cttttgcagc
     3781 attccaagtt aagcgtatca atcggttaag gaatgcgacg aaagaagtga cgaatggtaa
     3841 ttttgatgta cagctccccg tacatgacaa agatgaattt gatgagttag cagatgattt
     3901 caataaaatg acgaattcat tgaaagaatc tcaagcagaa attgaagaac aagaaaatcg
     3961 tcgaagacaa tttatggcag atgcttctca cgagatgaga acacctctta cgacaatcaa
     4021 cggacttctg gaagggttag aatacaatgc aatccctgaa aatcagcgag aaaacgcgat
     4081 taaattgatg aaaaatgaaa cagagcgtct gatccgtttg gtgaatgaaa acttggatta
     4141 tgaaaaaatc cgtacgaatc aaatcagtat ggtcattaaa aaattcaatg gaacagaaac
     4201 attgaaaaat attgttgcac agttagaagc taaagcagaa gcagcaggag atacattgac
     4261 attgaaagcc gttgatgata ttgatgttta cgctgattat gaccgatttg tccaaatcat
     4321 ggtcaatatt attcaaaacg cgatacagtt tactgaaaac ggccaaatca cgattacttt
     4381 agaaaaaggc tatcttgaaa cgatcatcac gattgaagat acgggaattg gaatgtctga
     4441 acaacaaatg aaaagtatct gggatcgtta ttataaagta gatccttcaa gaaaaaatac
     4501 aaaatatggt gaatcaggat taggtttacc aatcgtgcag caacttgtac gcttgcataa
     4561 aggaaaatta gaagtagaaa gcgaactagg aaaaggaaca aaattcgctg ttcgtttgcc
     4621 tgacgtagaa atcaaaaaag agtaaaaaac aagcggtata aaagatcatg aatcgatctt
     4681 ctataccgct tgtttttact ataaaatgat tgatttagcc cgcgggctct aaacgatcaa
     4741 tataaggact ttgagcaata gtagcatcat gagtaaagag taagatacct tgattttctt
     4801 catgagctaa ataagaaagc aagtggagta gctcttggat ctccaataca ctaagttcat
     4861 caaatgtatc ttcaataaca atattgtttc gcttggaaag caaggcatga agtaattgca
     4921 gctttacttt ttcaaaagac gtcaactcag ctgccgcaca attaagcaag gaaggagaga
     4981 tatcaagttt ttcttgatag gccaaacgat cttgtttgat ttctttttcc ggtacatcca
     5041 acaggacatg gtctttaaga ttcagataag gaaacatcgg ccaatttttt tggatgatcg
     5101 caaaattatc cagttttgca acaagaacct cttttttcaa gtgctccaat attttatgct
     5161 gtttttcgag aggacacaaa ataacggtca agtgttcagc atgaatcaaa tattcaggtt
     5221 tcattacgtc caggcctcct actttgcaaa tgatggataa aaaaagtgaa agggaataga
     5281 ctaagaatga gacagcatcc actgagccca ataagagttt gggcaaatcc cttaagtata
     5341 tctgtgaaaa aaagatcatt ttggaaatcg atggtaaaaa aagtctgttg atccatcgga
     5401 attgtaagtc cgcttaccga agtcaagata tcacggatat ctggcagttc agtagtcttt
     5461 aagacaagaa aattgatcct ttgaagaagc gcttcataaa ctgactggaa gcttacgatc
     5521 cacaataaaa ccaagataca acaaagaaag agaggaaaga acagctgcca gaaaataaaa
     5581 aggaataatt tggttttaga aagaccagtc aggtgccaat tgatcaattc ctgcttgttt
     5641 tgcctgactg aatagtaaaa gaaaaagata aaaaggaagg caaatagaaa aaataaactg
     5701 aatatgcctt ttatataaaa tcggctagct aattctaaag ccggaagaag ctctgtagat
     5761 gcagaagcgc caataaatga aagccgatct tggacttgat tggctaaagc cgattgcaaa
     5821 tggtaaagag tcaggcatgc tatcaataaa aaagcagaaa agcttgaaaa taaacaatag
     5881 agtattgtct gctttttgta ataaattaat cgagcagtag catagcggat tattttcata
     5941 cttttcacct ctataaaaag tatagaaaat gaatatgaat aaagtatgaa attcctttag
     6001 tgaaatcacg aattaaaacc agaaaatatt ttgtaaaaag caagaggttg tgaagaagct
     6061 gccctattct attaccgtag atgcaagctt ttgaacactg tttattcgaa aagaggagca
     6121 gcgacaataa
//
LOCUS       DACDNO010000110         6068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDNO010000110 DACDNO010000000
VERSION     DACDNO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.110"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            832..1566
                     /locus_tag="IWI22_002681"
     CDS             832..1566
                     /locus_tag="IWI22_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9170003.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IWI22_002682"
     CDS             1582..2751
                     /locus_tag="IWI22_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9170004.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IWI22_002683"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IWI22_002683"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9170005.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IWI22_002684"
     CDS             3841..4767
                     /locus_tag="IWI22_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9170006.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IWI22_002685"
     CDS             4786..6036
                     /locus_tag="IWI22_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9170007.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2027 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacaa caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agttaagt
//
LOCUS       DACDNO010000111         5682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDNO010000111 DACDNO010000000
VERSION     DACDNO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.111"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..1287
                     /locus_tag="IWI22_002686"
     CDS             133..1287
                     /locus_tag="IWI22_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9170008.1"
                     /translation="MEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSIN
                     GELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQ
                     REMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKA
                     GTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEY
                     RSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1385..2758
                     /gene="tet(L)"
                     /locus_tag="IWI22_002687"
     CDS             1385..2758
                     /gene="tet(L)"
                     /locus_tag="IWI22_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP9170009.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            3322..4584
                     /locus_tag="IWI22_002688"
     CDS             3322..4584
                     /locus_tag="IWI22_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP9170010.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            4829..>5682
                     /locus_tag="IWI22_002689"
     CDS             4829..>5682
                     /locus_tag="IWI22_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP9170011.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         1955 a          805 c         1167 g         1755 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta tgtggattct acgacacatg aaattatact ttctttctta
      541 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      601 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      661 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      721 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      781 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      841 tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
      901 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
      961 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
     1021 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
     1081 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
     1141 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
     1201 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
     1261 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
     1321 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggaatt
     1381 aattatgaag tgtaatgaat gtaacagggt taaatacaac caaattttaa tttggctttg
     1441 cgttttatct ttttttagcg tattaaatga aatggttttg aacgtctcat tacctgatat
     1501 tgcaaatgat tttaataaac cacctgcgag tacaaactgg gtgaacacag cctttatgtt
     1561 aaccttttcc attggaacag ctgtatatgg aaagctatct gatcaattag gcatcaaaag
     1621 gttactccta tttggaatta taataaattg tttcgggtcg gtaattgggt ttgttggcca
     1681 ttctttcttt tccttactta ttatggctcg ttttattcaa ggggctggtg cagctgcatt
     1741 tccagcactc gtaatggttg tagttgcgcg ctatattcca aaggaaaata ggggtaaagc
     1801 atttggtctt attggatcga tagtagccat gggagaagga gtcggtccag cgattggtgg
     1861 aatgatagcc cattatattc attggtccta tcttctactc attcctatga taacaattat
     1921 cactgttccg tttcttatga aattattaaa gaaagaagta aggataaaag gtcattttga
     1981 tatcaaagga attatactaa tgtctgtagg cattgtattt tttatgttgt ttacaacatc
     2041 atatagcatt tcttttctta tcgttagcgt gctgtcattc ctgatatttg taaaacatat
     2101 caggaaagta acagatcctt ttgttgatcc cggattaggg aaaaatatac cttttatgat
     2161 tggagttctt tgtgggggaa ttatatttgg aacagtagca gggtttgtct ctatggttcc
     2221 ttatatgatg aaagatgttc accagctaag tactgccgaa atcggaagtg taattatttt
     2281 ccctggaaca atgagtgtca ttattttcgg ctacattggt gggatacttg ttgatagaag
     2341 aggtccttta tacgtgttaa acatcggagt tacatttctt tctgttagct ttttaactgc
     2401 ttcctttctt ttagaaacaa catcatggtt catgacaatt ataatcgtat ttgttttagg
     2461 tgggctttcg ttcaccaaaa cagttatatc aacaattgtt tcaagtagct tgaaacagca
     2521 ggaagctggt gctggaatga gtttgcttaa ctttaccagc tttttatcag agggaacagg
     2581 tattgcaatt gtaggtggtt tattatccat acccttactt gatcaaaggt tgttacctat
     2641 ggaagttgat cagtcaactt atctgtatag taatttgtta ttactttttt caggaatcat
     2701 tgtcattagt tggctggtta ccttgaatgt atataaacat tctcaaaggg atttctaaat
     2761 cgttaaggga tcaactttgg gagagagttc aaaattgatc ctttttttat aacaggaatt
     2821 caaatctttt tgttccatta aagggcgcga ttgctgaata aaagatacga gagacctctc
     2881 ttgtatcttt tttattttga gtggttttgt ccgttacact agaaaaccga aagacaataa
     2941 aaattttatt cttgctgagt ctggctttcg gtaagctaga caaaacggac aaaataaaaa
     3001 ttggcaaggg tttaaaggtg gagatttttt gagtgatctt ctcaaaaaat actacctgtc
     3061 ccttgctgat ttttaaacga gcacgagagc aaaacccccc tttgctgagg tggcagaggg
     3121 caggtttttt tgtttctttt ttctcgtaaa aaaaagaaag gtcttaaagg ttttatggtt
     3181 ttggtcggca ctgccgacag cctcgcagag cacacacttt atgaatataa agtatagtgt
     3241 gttatacttt acttggaagt ggttgccgga aagagcgaaa atgcctcaca tttgtgccac
     3301 ctaaaaagga gcgatttaca tatgagttat gcagtttgta gaatgcaaaa agtgaaatca
     3361 gctggactaa aaggcatgca atttcataat caaagagagc gaaaaagtag aacgaatgat
     3421 gatattgacc atgagcgaac acgtgaaaat tatgatttga aaaatgataa aaatattgat
     3481 tacaacgaac gtgtcaaaga aattattgaa tcacaaaaaa caggtacaag aaaaacgagg
     3541 aaagatgctg ttcttgtaaa tgagttgcta gtaacatctg accgagattt ttttgagcaa
     3601 ctggatccag gagaacaaaa acgatttttt gaggaaagtt ataaattatt ttccgaacga
     3661 tatggcaagc aaaatattgc ttatgcaaca gttcataatg atgagcaaac ccctcacatg
     3721 catttaggtg ttgtgcctat gcgtgatgga aaactgcaag gaaaaaatgt gtttaatcgt
     3781 caagaactgt tatggctaca agataaattc cccgagcata tgaaaaaaca gggttttgag
     3841 ttgaagcgtg gtgaacgtgg ctctgaccgt aaacatattg agacagctaa atttaaaaaa
     3901 caaactttgg aaaaagagat tgattttcta gaaaaaaatt tagcagttaa aaaagatgaa
     3961 tggactgctt atagcgataa agttaaatca gatttagaag taccagcgaa acgacacatg
     4021 aaaagtgttg aagtgccaac gggtgaaaag tccatgtttg gtttgggaaa agaaataatg
     4081 aaaacagaaa agaaaccaac caaaaatgtt gttatatcgg agcgtgatta taaaaactta
     4141 gtgactgctg cgagagataa cgataggtta aaacagcatg ttagaaatct catgagtact
     4201 gatatggcga gagaatataa aaaattaagt aaagaacatg ggcaagttaa agaaaaatat
     4261 agtggtcttg tagagcgatt taatgaaaat gtaaatgatt ataatgagtt gcttgaagaa
     4321 aacaagtctt taaagtctaa aataagcgat ttaaagcgtg atgtgagttt aatctatgaa
     4381 agcactaagg aattccttaa ggaacgtaca gacggcttaa aagcctttaa aaacgttttt
     4441 aaggggtttg tagacaaggt aaaggataaa acagcacaat tccaagaaaa acacgattta
     4501 gaacctaaaa agaacgaatt tgaactaact cataaccgag aggtaaaaaa agaacgaagt
     4561 cgagatcagg gaatgagttt ataaaataaa aaaagcacct gaaaaggtgt ctttttttga
     4621 tggttttgaa cttgttcttt cttatcttga tatatataga aataacgtca tttttatttt
     4681 agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt tttaaagtat tgaaaaccct
     4741 taaaattggt tgcacagaaa aaccccatct gttaaagtta taagtgacca aacaaataac
     4801 taaatagatg ggggtttctt ttaatattat gtgtcctaat agtagcattt attcagatga
     4861 aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa gtgagaccat ggagagaaaa
     4921 gaaaatcgct aatgttgatt actttgaact tctgcatatt cttgaattta aaaaggctga
     4981 aagagtaaaa gattgtgctg aaatattaga gtataaacaa aatcgtgaaa caggcgaaag
     5041 aaagttgtat cgagtgtggt tttgtaaatc caggctttgt ccaatgtgca actggaggag
     5101 agcaatgaaa catggcattc agtcacaaaa ggttgttgct gaagttatta aacaaaagcc
     5161 aacagttcgt tggttgtttc tcacattaac agttaaaaat gtttatgatg gcgaagaatt
     5221 aaataagagt ttgtcagata tggctcaaag atttcgccga atgatgcaat ataaaaaaat
     5281 taataaaaat cttgttggtt ttatgcgtgc aacggaagtg acaataaata ataaagataa
     5341 ttcttataat cagcacatgc atgtattggt atgtgtggaa ccaacttatt ttaagaatac
     5401 agaaaactac gtgaatcaaa aacaatggat tcaattttgg aaaaaggcaa tgaaattaga
     5461 ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa aataaatata aatcggatat
     5521 acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt ttatgaccga
     5581 tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac accgtaaaag
     5641 gttaatctcc tatggtggtt tgttaaaaga aatacataaa aa
//
LOCUS       DACDNO010000112         5563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDNO010000112 DACDNO010000000
VERSION     DACDNO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.112"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(29..580)
                     /gene="raiA"
                     /locus_tag="IWI22_002690"
     CDS             complement(29..580)
                     /gene="raiA"
                     /locus_tag="IWI22_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9170012.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(708..1427)
                     /locus_tag="IWI22_002691"
     CDS             complement(708..1427)
                     /locus_tag="IWI22_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9170013.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(1424..2737)
                     /locus_tag="IWI22_002692"
     CDS             complement(1424..2737)
                     /locus_tag="IWI22_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9170014.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(2818..3138)
                     /locus_tag="IWI22_002693"
     CDS             complement(2818..3138)
                     /locus_tag="IWI22_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9170015.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(3246..3533)
                     /locus_tag="IWI22_002694"
     CDS             complement(3246..3533)
                     /locus_tag="IWI22_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170016.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3660..4304
                     /locus_tag="IWI22_002695"
     CDS             3660..4304
                     /locus_tag="IWI22_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9170017.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(4363..5070)
                     /locus_tag="IWI22_002696"
     CDS             complement(4363..5070)
                     /locus_tag="IWI22_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9170018.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
BASE COUNT         1605 a         1168 c          958 g         1832 t
ORIGIN      
        1 gttttcgttg ttatttaagt aaggctgatc agtctgtttc aatcagtccg tattttccat
       61 ctttacggcg gtaaacgata cttgtaccat ttgtttctgc atcttcaaaa ataaagaagt
      121 tatgtcccag catgttcatt tgtaagactg cttcttcact atccattggt ttcaatgaaa
      181 ggcgtttcgt acgtacgata tctaattcag aatcattttc gttttcgtcc tcttcattga
      241 ataaagcagc agcatctgca gtattcatgc ccgtttcacg ggatttacga ttaatttttg
      301 ttttgaattt gcggatttgg cgttccaatt tatcaacaac caaatcgata cttgcgtata
      361 aatctggaga agtttcttcg gcacgtaata caagataagg taatgggatc gttacttcca
      421 cttttgctgt tttttctgta taaactttta agttcacatg cgccgttgca tcgggtgcgt
      481 cactgaaata gcgttctagt ttgcctactt ttttctcaac gtagtcgcga atcgcttcag
      541 ttacttcgat attttcgccg cgtacattat atctaaacat aaaaattacc cctttcgtct
      601 accacaatca agaaggagaa ggaccactct ttccctcttc ttaattctat tatatcgaac
      661 tctaggttat ttgcaaatta aatcgctatc aaatttccac atctaattca tctcgccaaa
      721 gaaatggttc ggatgatttt cggctgtgcg acctgcaaac aatctgctgc gtggaaaaga
      781 gtctgtcctg ttgtatagac atcatcaata atcaatattt ttttattttg tatcttagaa
      841 aaggtgactg caagttcaaa aggttgaggc atctccaatc tttcctttcg cgtgtttttt
      901 gcttgagcca gcagatgctt ctctcttttg agtagcatct gaaaaggaat tcttgctgct
      961 ttcaacatct cgcttacttg attgaatcct ctttctgttc gccttttttc agataaaggt
     1021 attggacaat agatgtatgt cgggtattct ttttttatct gcgtccattt atatgcaaag
     1081 gtgcctgcta gtcggatatc tcccataaac ttgtattgat tgagccaggc cttcatattt
     1141 tcatcgtaca caaaaaaggc acggtgctga aaatcatagg tcggatattt cttcttccat
     1201 tcttgacagt cactgcagta aacgggaata gactttactt tctttttcgt actctctaga
     1261 ttgaaatgga ttggtttttg acagccagaa cagcctcttg tttccaataa atgaaattgt
     1321 gcgcgacaaa ctgaacaagt ctgaggtaat ctgtatttcg aaacaccaat aatctcgctg
     1381 aatgttaaat tttggaagat atctttactg cagcaactgc acctcacagc atcttcttcc
     1441 cttcttcatt catttctttg atttcctgac aagcttttcg aatagctgca gttttttctt
     1501 catagaaaaa gtatacttga ctgttcgtat aaggcccttt acgatcggct cttcctgcga
     1561 tttgtaccaa agaagattta gtaaaaaccg gatgatccgc tcctaaaacg ataacggata
     1621 ttctttcaaa tgtgactcca cgttcaagga tcatcgtggt taacagtata tcatattttg
     1681 cttctcgcat tttctggact ttttccgccc gctgattgtc tcttgaagaa acatctgtga
     1741 tctcatatct cggaagagcc tcttgaagtg tggctttcaa acgatgcatc atcgtgatac
     1801 ttggacaaaa taaaaggata tggttttctt taattaattg atgaatgatc cgcaccaaag
     1861 attttatctt cttcttgttc ttgcattttt gtcgccaatt attcatgaaa accaagacgg
     1921 gttcaggaag caaacgccga tgaaagcgta aagcaagttt gtggatgtca aacgtctgct
     1981 tgatttcctt tagtaacttt ttatcaggtg tggcacttaa gtagatgaat cgtccgtctg
     2041 gtttaatcgc tgtttgcaca gcatgagcta atcctgcatc cccgttataa ggaaacgcat
     2101 cgatctcatc gatcactatt aaatcaaagg accgataaaa acggtatagc tgatgaatcg
     2161 tacagataac aaaagaagta aaggtataag ccgttgctgt attcccatgg agcaacgtga
     2221 ttttttcttc tggaaaaaca taacgatagc gtaaaaacaa ttcattacaa acatcgactc
     2281 gcggtgtaca aatcgctact cttcctccag cttttaaagt ttccactaaa ataggataaa
     2341 gcatctctgt tttccctgca cctgttacag cccaaataag atgatgtctt ttttctgaat
     2401 ggcaaagtat tctctttgag atttccgttt gtttttcagt aagctgtcct gtccaaaaaa
     2461 agacgacttc tcgaggtttt gcttcaggct gatcaaacaa atataaatct tgttgagtgt
     2521 cgcatcgccc taaatataga caatgtgtac aaaaataacc gtaatctgca agctgtccat
     2581 caatgacgga cgtcctcatt ccacagcgat gacaaagaat ccagttgccc aaacgttcca
     2641 ctgctgcgat cttctcataa tttggtaaat ttacctcctc acagtctatt gcttcaatat
     2701 ctctcgtcag aagccttcta ccccaaaaat gattcatatt cttcgcccta aaaaaatata
     2761 cgaaaaaaca agcgatctat tttttggatc gcttgttcga tagttatact gttctattta
     2821 ttcacctatg agcgtgattg cttgatctaa cactttttcg ccattcatca taccataatc
     2881 agccatattg atgacatcta aaggaatgcc ttttggttcc agacgtttct caaaatcagc
     2941 tttcataaag cgaacttgtg ggcctaataa caacacatcc acgtctttat ttgcaaggtt
     3001 gttatctgcg tcagaagcgg acacagcgaa aatatccgag tcgattcctt ttgcttcagc
     3061 agctttttgc atttttgtta ctagtaaact tgtgctcata ccagccgaac atacaagcat
     3121 gattgttttt ttagccatac ttttctctcc tttttaaaac aaactatgca aacgttttct
     3181 tcgcgtctgt tactctatta tataaaaaga aagaaatttg tcaacgtttt ctaaacgcgg
     3241 tttatttaat caaatagcca actagatttc actaatttat tgaacacgac atattctttg
     3301 tccaaattga tgacaactac ttcatagcct gcatttatcc agccataggc acctttttca
     3361 gggtgtttat aatcatttgc ccaccaatct gtactttccc ttgctgtttt gggaagtccg
     3421 ttactgggaa acagcagttc gtcaaattgg gtaaaggtca acgttacttg agagccgcca
     3481 ttgttcttca aataatgcgt aattgcatcg tactttttca ttcgttcctg catccccaga
     3541 tccacctgcc tttgtcttta ttatcgcata ctacagcaat tgcgcaaccg ttacagtgtt
     3601 gaaacaacct agaactttat ttatactgaa taaagaaaat gaaaaaaaga ggtgacaaga
     3661 tgatcgagcg ctatcgtaca ataaaagaaa atggacaaag tgagattgag atcaaaaaat
     3721 cacgctttat ctgttctctt agacgaatag aatcagagga ccaagcaaaa agtttcattc
     3781 aagaaataaa aaaagaacat tggaaagcga accataactg cagcgccttc atcattggag
     3841 aaaaaaatga aatccagcga agcagcgatg atggagaacc aagtggaacg gcgggcgtac
     3901 cgatgttgga agtcctcaaa aaaaatcaac tgatcaatgt agctgcagta gtcactcgat
     3961 attttggagg aacgaaactc ggcacaggcg gattaatacg agcatacact catgcggtct
     4021 ctcaagcatt gtctgacatt ggaatcgtag aaggtacctt gcaacaggag atctctgtga
     4081 tgatcacgta tccgcaacta ggaaaattac agaattatct tgaactccaa cattttttta
     4141 taaaagaaat ccaatatact gatcaagtta gcgtcatctg tgctgtagat gaaaaaagtg
     4201 ctgaccactt taaagaatcc atcattgagc tgctgaacgg acaagtccaa tttgaagagg
     4261 gttctgtttc ttatcacgag caactgataa ctgatcatat ataaaaacag gtgattctga
     4321 ttctctttta tttctaataa ggaatcaggg atcacctgtt ttttattttt ccaaataaaa
     4381 ctcaaaacga tttcctacgt attgcgtacg cacatactca aacggcgtac cgtcttcaaa
     4441 ataagaaatc tggcgcaaac gaagaactgc atctcctcgt tttacctcta aatagtcagc
     4501 aattttctca gaagccaaca cagcagtaat cgtttgattc gctggaccga tctttttccc
     4561 gcctttttct tctagcgtgc gatacaacga ttcagtaatc tctgctttgc tgtagccatc
     4621 gatcagcttt tgtggaacac tggcaacttc aaaacaaata gggacatcat ctgcgaaacg
     4681 aatacgttcc attcgcaaga tcgtgtcatc tttactcaag tttagttttt ccatttcact
     4741 tgagcttggc gaagtcaaga aataagagat tgtccgacta gacggtactc gattttgtga
     4801 ttccataatt tctgtaaaac ttgttgcacc agacattttt tcttgtactt tttctcgcgc
     4861 aacatacgta cccgaaccga tctttctttc taagattcct tcatctgcca agttttgaat
     4921 tgcttgtctc aacgtcatac ggctgacatt gaatttcact gctaattctc tttctgaagg
     4981 aagacggtct ccgactttcc atataccctg ttcgatttca ttttttaatt ggtcatgaat
     5041 ctgtatatat acaggtaatt gattcgacat aacgtacacc cgtcctttcg tttcttctat
     5101 tataactggt atatacctga ttcacaactg aaaaagataa aggtttctat ccatcaacag
     5161 aggatttaga cacacatcgt tctccctact cctattgtca gaaacaattt tttagatatg
     5221 ttcaacctag aaaataacaa aagaccctaa aatcgatgaa aatgccgact tatggatctt
     5281 ttgttattat tagcggtttt tagccatcca tttttttgta tgattcgttg ggcgttttga
     5341 ttttacctgg accgcttttc cgcctttttt cggaatattc acttcaatat tgtatttttc
     5401 tgatttgacc aaatcatata acttttcagc tgcttcgtct actttttctt tccatttcat
     5461 gtggatccct ccaattcttg tagttaactt aactctatct taattcagca gaatcttcaa
     5521 ggaacaaggt agcaaagcaa aaaaaagagc tctcgaacta acg
//
LOCUS       DACDNO010000113         5474 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDNO010000113 DACDNO010000000
VERSION     DACDNO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.113"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            393..1616
                     /locus_tag="IWI22_002697"
     CDS             393..1616
                     /locus_tag="IWI22_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9170019.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGRTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGTEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            1651..2418
                     /locus_tag="IWI22_002698"
     CDS             1651..2418
                     /locus_tag="IWI22_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9170020.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            2610..3008
                     /locus_tag="IWI22_002699"
     CDS             2610..3008
                     /locus_tag="IWI22_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9170021.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(3314..3709)
                     /locus_tag="IWI22_002700"
     CDS             complement(3314..3709)
                     /locus_tag="IWI22_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170022.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(3710..4087)
                     /locus_tag="IWI22_002701"
     CDS             complement(3710..4087)
                     /locus_tag="IWI22_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170023.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(4077..4472)
                     /locus_tag="IWI22_002702"
     CDS             complement(4077..4472)
                     /locus_tag="IWI22_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9170024.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(4539..4724)
                     /locus_tag="IWI22_002703"
     CDS             complement(4539..4724)
                     /locus_tag="IWI22_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9170025.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(4806..5060)
                     /locus_tag="IWI22_002704"
     CDS             complement(4806..5060)
                     /locus_tag="IWI22_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170026.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISTKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(5082..5327)
                     /locus_tag="IWI22_002705"
     CDS             complement(5082..5327)
                     /locus_tag="IWI22_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170027.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
BASE COUNT         1866 a          841 c          914 g         1853 t
ORIGIN      
        1 tataatatat attttaatct ttaaaacttt agcaacctcc aaaaaggctt tatatcaatc
       61 ttaaatagat tttagggtgg acataaagcg atgtgaatgt ggacaaaaag cgatgtggta
      121 ttccgaatat atccaaaaaa agaaaaagta tgtgaattta tttctaaaaa ctagaaaaat
      181 atttgttttt taattttggt aatttttata atttcgtatt tttttgaaaa ggatttttat
      241 tttgaaaatg ttctaaaata gcgatttctg ctgtattttc aatgaaattt gattggaaaa
      301 aataaaatgt gttttactga cattgagtta tgttataaat ttacgataca tttcttattg
      361 taacgtatcg aaaacaggtc tatacttatt caatggaatc gtaccagttc caagttttta
      421 agctcgtacc aagcttagaa agaagaggca atcgtatgaa aataacagaa aagaatgtaa
      481 acccgaaatt tgaaactacc gaaagcaatc taaaaaagtt tgatttaact gttactttat
      541 cgaatcaact tgtagatagc attgttgaaa tggatgctat gccacaaaaa atttatcttt
      601 acgctatagg acatcttgac cctaaaaatc cgccttttaa taataccgtc aaagtatcta
      661 aaaaggcttt atttgcgttg atgtcttcta gttcatcttc ttcaaaacat tcaagattaa
      721 aacaagttat tgaaaaattg catcagcaat ctgtcattaa aattcaaaga actaaaccta
      781 acggtaagac tgaatacaaa gtcatctcac cgctagaaat tactgaattc aatgattttt
      841 ctgatgcagt atctattaaa tttagtagtt caattatgcc tttattaacg aacttagagg
      901 atcattttac gcaatataca tttgatcaag tagttaactt gacaagcaag tatgcaatca
      961 gtttttttca attattcact aaagatttta atctgtatga agcaaataga gataacttaa
     1021 catggagtac aaaatcactt gatagattaa aaaatcctgt tttctccatt gcagatttac
     1081 gaaagaaatt cagtattgag aaaaaatata aagcttttcg agactttgaa agacgtgtaa
     1141 ttaaagaacc gattgcccaa ctgaataaat acactgattt taaaattgac tatgagctta
     1201 tcaagaaggg tcgtacttat acaaatataa agttcttgct tacacgtacc atgtcagttc
     1261 cggtacctta taagcaagaa caaagagacc ctgcttattt aaaaggcaca gaagataaaa
     1321 cagagaaaat gaatcaatcg tacttgaaag ctatgaatag caaatacact aaattactaa
     1381 tcaaaaactt tttgatcagt tctgccgact tatgcgatct taaacttatg agccgacttc
     1441 agaataaagt ttatccgctt tatgatcgtt tatccaaaga tagaggattt gaatcagttg
     1501 tcaatcacct atcttatgtt gctactcata aggaggatta cacaataaaa aatactgtta
     1561 aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat gattaacaac
     1621 tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac aaagtacaat
     1681 gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga actttatcaa
     1741 ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt agatttacct
     1801 gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga actaaaaacc
     1861 atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga caaaaaatat
     1921 aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga agagcatagt
     1981 gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa aaagaagtat
     2041 ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat cgtgttatac
     2101 aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg agattttgag
     2161 aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga gattacgaaa
     2221 acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag ggaatcagtt
     2281 gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag aaaaggcaga
     2341 aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa aacttataga
     2401 gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct tcagacgtat
     2461 gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt taattagctt
     2521 gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa aaagcaaaaa
     2581 ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca gtattcgaat
     2641 ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat acacatggtg
     2701 aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac ttggtgcttt
     2761 tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta aatgcaagaa
     2821 tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa gttgctaaag
     2881 ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat ttaaatgttt
     2941 tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg agagaaaagt
     3001 tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta ggacgtatgg
     3061 atttaagaaa gacaaattat caaagaatat attaaaagct ctcagaagcc ttctgagagc
     3121 ttttaaattg ctatgtattg tactgcatct gctcaaattg attttctttt catgcttacc
     3181 atgtatctag ttttccatca agtaattttt agatatgtat taagtaataa atagcaaatt
     3241 tgcttgatat aagctcgaat taggtgcttt ataatgtgtt aggataaatt atacaagaaa
     3301 atgtcaaaag ctctcagaac gcttctgaga gctttttatc ggtaggcttg tttaacgtga
     3361 tcttgaaaga tacccttttg atgtttctct cgatccctgc atcgctaatt actaagtgtt
     3421 cttcaagtgt gatgtaggta tataaattaa gcatcaaaat ggtgtagata agcaaaaaac
     3481 tccctaaaac taagtgttga ttataaaaat ctgacttata aaaaagaact gagaatgtta
     3541 gtgttgattc ccaaaaaaga aacaaaattt tgtatttcca aagatagtgg atcaagcgct
     3601 gtgctttacc catctcaaat aatttataat aacctgctaa ttgatcagca agcgtatcgt
     3661 taacggtttc tgtttgtgtt tgtttcatct ttctcttctc ctgtttcaat taatctaaaa
     3721 tttcagattg agcaagcaac tcattgtggt agtcggtttg aaattcaaat aacaattttg
     3781 ccttcttaac gaggtttaaa gcgttcgtta gtgattcact taatgcttca acatctttaa
     3841 ctgattttgc atgtttcatt ttttcagtgt gcgtatagca gaattcaggt attgagttag
     3901 gggttgcctg ttcaataaac atctcaattc gcaagttatt atcctcaaaa ataattagat
     3961 cattggcaaa gtgtttggta ctaacgctaa tgccagttgt atcttttaat agattattga
     4021 ttaacgttcg agagaaatag tttaagtgtg tagatttcat aggttcggtc tcctttttac
     4081 ttttcaagca actcttcttc tagtgctttt tgaagtagtt gagagaagtt tatgtttttc
     4141 tccttcccta aaagaactaa ccattccgga acactaacat tttttctaat tgtttttgta
     4201 tttaagcttc ttcggaaaag tttaatgttt agttggatga ttactaccac ttggttggct
     4261 ttcaaagtta attcttccac tgctgatggt ttaggaaatt ctgcatctgg tacactatat
     4321 agcgctaatc ctaaagcatc cttagcgttt aaatatgctt cttcaagact atttccaaag
     4381 gtagatattt ccggtaagtc agggaaaacg atgttataag cttctccttc tttctcgaag
     4441 attgccggat aaaaaatatt ttttgtcttc atttcctcgc tccttttcta gtgtaagaga
     4501 ggatagtatg cctaatacca aataggaagg caaggctgtt aaagccctgc ttgcttccag
     4561 atattagctt ctgttcctag tggtacatca ccattgtgga tcggaacaac gaccgttctt
     4621 ttagtcttgg aattataaaa ttttgcatga ctgccagttt gtccagtctt tttgaagccg
     4681 ttagatttga gaatttttac aatttctctt gctgtcttag gcatagctac cctcccttta
     4741 caaaatctat tatacacatt gttgtgtatg taatcaatag aaaaagagga tgttttattg
     4801 caaaactaat aaaatgattt attggttttt tcagcaaatt gaatgttatt agcgttgcaa
     4861 atattcataa tttcccaagt atcattagaa gtataggtat ctaagaattc aatgacagtt
     4921 acggttgatt cgtatggttc ataagccaaa gccttctcca agtcttttgt tgaaatgatc
     4981 aagacttttt caatttcctc cccttcttca tttgtgaaaa cgtattccaa agtgatttgt
     5041 tcttcatcgg tataattcat attgtgttct cctttcttgt attaagaaaa atggatattt
     5101 ccaattgcat cccactcggc accattgtgg tataagacgt gaccgagcgt tacgcctttg
     5161 aaatgctctt ctaaatacat ccaaacgcat tcaaggctag tacctgcttt gagattgtag
     5221 aagtctgcat ccaatacctt ttcagaagtt atcggatcaa tgcgagttgg tacctcaata
     5281 aattcagtcc aaatttctgc aaaagtctta ccttccaaag tccacactcc ctatcaatta
     5341 attccaaaag caaatagaga aatcaagcga ccgtcaatag tcacttcaac aactgaaaat
     5401 gccgttgaaa cagttttaac atccacactt tccaatttcc cgatgaagga atttttgtgc
     5461 tttagaatag cttt
//
LOCUS       DACDNO010000114         5473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDNO010000114 DACDNO010000000
VERSION     DACDNO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.114"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..546)
                     /locus_tag="IWI22_002706"
     CDS             complement(<1..546)
                     /locus_tag="IWI22_002706"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170028.1"
                     /translation="MNIITRDELIYAYKDEVNLLEVSQYRSSNVITLNIDTVSEFIKL
                     AKELKINHVYYNYLYYSIDNYLIPLDTYDEFSDEINIEVKKHNEVVDSLDFSKPKQLT
                     LLIVLNGTIMEITLNHFWIKESGIPDNETKHSEIQDKFFKDFKLQQEEISRNEHDDKN
                     RLKEYILSDNEFSYMKNQRLRQ"
     gene            complement(651..1214)
                     /locus_tag="IWI22_002707"
     CDS             complement(651..1214)
                     /locus_tag="IWI22_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017550078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9170029.1"
                     /translation="MSGYIYGYARVSTRSQELNRQIDLLNEQNCNEILTEKMTGTKAD
                     RPQLNRLKDKVRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHEVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQRDIERAVKLYDSQ
                     IYSIKEITEMTGISKATLYRYLKTNNF"
     gene            1590..1820
                     /locus_tag="IWI22_002708"
     CDS             1590..1820
                     /locus_tag="IWI22_002708"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170030.1"
                     /translation="MLKNLFFKNKKQLTILEKIFYFFVGNKNRMQNKSYYVRERAGAK
                     WRYRKFKDPWIAKNRNTLFKRVFGFFEDIFRG"
     gene            complement(1855..2004)
                     /locus_tag="IWI22_002709"
     CDS             complement(1855..2004)
                     /locus_tag="IWI22_002709"
                     /inference="COORDINATES: protein motif:HMM:NF025625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9170031.1"
                     /translation="MIEKNGVLRTILDISYNSPSRVAELISGNSTNGWSFVEGLSELR
                     TMDQE"
     gene            2079..2342
                     /locus_tag="IWI22_002710"
     CDS             2079..2342
                     /locus_tag="IWI22_002710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170032.1"
                     /translation="MILQVYNEQQGLTGGSSESSSSPESSEPESAAPSTPAGGDVSSV
                     DTNGNGNVTIKEAKAAGYSMPITSDHWLYQYMNDRDNDGMVGE"
     gene            complement(2442..2957)
                     /locus_tag="IWI22_002711"
     CDS             complement(2442..2957)
                     /locus_tag="IWI22_002711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170033.1"
                     /translation="MIHVEQGLTDIQVVPTALVDSEWKFIKDLTIYLEENERIFEGKE
                     LYLIRNFSRKGIGFFEESGFYPDFIMWYFNGSQEYIIFIEPHGMLRENLDGNKVQLYK
                     EIKKVENEISLSEGTRPVLESFILSPTPFATLNNPINNPSNYSKGDLNRQHVIFMEDE
                     NYIEQLFESLI"
     gene            complement(3022..3537)
                     /locus_tag="IWI22_002712"
     CDS             complement(3022..3537)
                     /locus_tag="IWI22_002712"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010290771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HAP9170034.1"
                     /translation="MVPLTSEKIFGLAPIIDDQAKILILGSMPGKESLKKEQYYANKR
                     NQMWKIMENTLKLQLPEEYPKRINVLRKHHIALWDVIHSCKRVGSLDSAITEDVPNDF
                     GSFFKEYSSLKIVGFNGNKAYQTFKSKIGFQDFPEIQFIKLPSTSPIPGKNVKSFEEK
                     VVEWSKVVNSL"
     gene            complement(3846..4208)
                     /locus_tag="IWI22_002713"
     CDS             complement(3846..4208)
                     /locus_tag="IWI22_002713"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170035.1"
                     /translation="MADNLLNKNKGKRNLLERKEEVKPQQSFNRSSLFTVNESQQTNG
                     DQKKDVPRKKERGTTTTIRCATDTSHRLNAIVTSFNLDSVNELIEILLDNYEASLTTD
                     ERREIKTLLEVYQRKSKK"
     gene            complement(4250..5086)
                     /locus_tag="IWI22_002714"
     CDS             complement(4250..5086)
                     /locus_tag="IWI22_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9170036.1"
                     /translation="MAKVITFGNFKGGTGKTTNSAMMAYTLSKLGYKTLLADLDPQAN
                     ATSLYLHTKQRITNNIVTFDKTLMSAIADEDLSDIIIEIKENLFLLPSFADFTSYPLF
                     LEKKFPDSQFNRVTFLNELMTKIKDDYDFILVDVPPTLSTYTDSALLASDYTIIVLQT
                     QERSLVGAEAYVGYLQELIDNYNADFDILGVLPVLLKNNSKVDEATLNTAKEKFGEEN
                     LFENVVKNMERLKRYDIIGIVDPDLDTKYDIHDKRVMGLYKRVTEEMLKRLEEIESRN
                     KQ"
BASE COUNT         1716 a          972 c          840 g         1945 t
ORIGIN      
        1 ttgtctcagg cgttgatttt tcatgtaaga gaactcattg tcgctcaata tatattcttt
       61 taatctattt ttatcgtcgt gttcatttct agatatttct tcctgttgaa gtttaaaatc
      121 cttgaaaaat ttgtcttgta tctcagaatg cttagtttca ttatctggta ttccgctttc
      181 ctttatccaa aaatgattta aagtaatttc cattatagta ccattcagta caattagtaa
      241 ggttagttgt tttggtttag aaaaatcaag actatctacc acttcattat gctttttaac
      301 ctctatattg atttcatcag aaaattcatc atatgtatct aagggtatga ggtaattatc
      361 aatagaatag tatagataat tataatatac atgattaatc tttaattctt ttgctaactt
      421 tatgaactcc gataccgtat caatatttaa agtaattaca tttgaggatc gatattgact
      481 cacttccaat aaattgacct catctttata tgcataaatt aattcatctc tagttataat
      541 attcattaac tccacttcct acccgattat tatataatcc aaaaatatca cttcaatcaa
      601 ttattaattt ttcagaatta aagatgaata atttcatttc atgataagat ttaaaaatta
      661 tttgttttta aatagcgata gagtgtagct ttactgatgc cagtcatttc tgtaatttct
      721 tttatgctgt atatttgact gtcatacagt ttcaccgctc tctcaatatc tcgttgatta
      781 acgcgtgggc gtcctccttt tcggcctcta gcacgcgcac tttttaaccc ttctttggta
      841 cgctccacaa tcaaatcacg ttcaaactga ctaaacgctt ggaagacggt catcattaag
      901 cgtccgtgag gagtgctagt atcgaagttt tctttcaaac tcaccagctt cacttcatgt
      961 tcttcaaagt aggtaacgag atcaattaaa tctttggtac tccgacctag acgagagaaa
     1021 ctttccacga taaccgtgtc tccaggacgc accttatctt ttagtcgatt caactgagga
     1081 cgatcggctt tggttccggt cattttttcc gttaggatct cattgcaatt ttgttcattg
     1141 agtaggtcta tttgtcgatt caactcttgc gagcgtgtac tcactctggc atagccatat
     1201 atatatccac tcataaaaaa tcatcctttg tttttgatat tattgtatca taaacgggag
     1261 ttaaaggtgc atacataaag agagaagttt tgagactaaa aaggactact ttttaggtga
     1321 aaaatccgtc ctttaaaatc gtctcataat cgattgtttt tgagacttca ggtttaattt
     1381 taactgactt aattttttga gttattatct aaaagcttta agtttaagga gttgattatt
     1441 ttctggaaac aataaaccaa aatttgttta acctgttgtg ccatataatt tttacttagg
     1501 cacactttaa cgtattttta gtttaggggt atgctaatat agtagttaga tacttacaca
     1561 gtattaaggc tgagccaagg gggaaaggaa tgcttaaaaa cttatttttt aagaataaga
     1621 aacagctaac aattttagaa aaaatattct atttttttgt aggaaataaa aaccgcatgc
     1681 aaaataagtc ctattatgtt cgagaacgtg caggggcaaa gtggcgatat cgaaaattta
     1741 aagatccatg gatagcaaaa aatagaaaca ccctattcaa aagagtcttt ggtttttttg
     1801 aggatatatt tagaggataa atttccaaat aaaaaagagg cccatgcctc ttttttattc
     1861 ctgatccata gtccgcaatt cacttaaacc ttcaacaaat gaccatccat ttgttgagtt
     1921 tcctgatatt aattcagcaa ctctcgaagg actattatag gaaatatcaa gtattgttct
     1981 caaaactcca tttttttcga tcacttttcc atcttcaatg taactgtcaa tcacatcgtt
     2041 atagcgaata aagaaattac cagtcttcca ttgcgcactt gattctacag gtctacaatg
     2101 aacagcaagg tttaacagga ggaagtagtg aatcttcatc ttctcctgaa tcctcagagc
     2161 cagaatctgc cgcaccaagt actcctgctg gaggagatgt ttcctctgta gataccaatg
     2221 gaaatgggaa tgtaaccatt aaagaagcaa aagcagctgg ctattctatg cctattacta
     2281 gtgatcattg gttatatcaa tatatgaatg atagagataa tgacggtatg gttggagaat
     2341 aaaccaaaat aatgaaaaat gaaagaccag attaatgaac tgccccctgt caagtagaca
     2401 gggggcagtt cattcggaaa tttagtctct tttatctgtt tttatattag tgattcaaat
     2461 aactgctcaa tataattttc gtcttccata aatatgacat gttgtctgtt taagtcacct
     2521 ttagaataat tagatggatt attaattggg ttatttaatg tagcaaatgg tgtgggagat
     2581 agaataaagg actctagaac tggacgggta ccttctgaca atgaaatttc attttctacc
     2641 tttttaattt ctttgtataa ctggacctta ttaccatcta aattctctcg caacataccg
     2701 tgaggctcaa taaatattat atattcctga ctgccattga aataccacat gataaaatcg
     2761 ggataaaacc cagattcttc aaaaaatcca attccctttc ttgagaagtt acgaattaag
     2821 taaagttctt taccctcaaa aattcgttca ttttcctcta agtagattgt caagtcttta
     2881 ataaatttcc attcactatc aactaacgcg gtagggacta cttgaatatc ggttagtcct
     2941 tgttctacat gtatcaacgg gttatataaa gatttatgtt tcttttagta ggctctgatt
     3001 ttcaaatcaa tacctaaatg tttataagct atttactacc ttgctccact ctacaacttt
     3061 ttcttcaaac gattttacgt ttttccctgg aattgggcta gtagaaggta acttaataaa
     3121 ctgaatctct ggaaaatcct ggaaaccgat ttttgattta aatgtttgat atgctttgtt
     3181 cccattgaac ccaacaattt ttaaggatga gtattcctta aaaaaagacc caaagtcatt
     3241 tggcacatct tctgtgatgg cagaatctaa actccctact ctcttacaac tatgaatcac
     3301 gtcccataaa gcaatgtggt gttttcttag aacattaatt cgttttggat attcttcagg
     3361 gagttgtaat ttgagggtgt tctccataat tttccacatt tgattccttt tgttagcata
     3421 atattgctct tttttgagag attcttttcc aggcatggat cctaaaatca aaattttagc
     3481 ttgatcgtcg ataataggag ctaaaccaaa tattttttct gaggttaatg gaaccatatt
     3541 tttatcccct ttgtagttca gtcacataag agttaatttt ttaaaacgtt atggtatcta
     3601 atccttgtag tatactagaa tgtttttttt agcttttttt ggtgtctatt gacgacttta
     3661 tcttcgtata tttgaactaa atcctcaaaa cgtttggtgt cactattgtt taaattttca
     3721 acgtataagt ttacaaaagt ccttcccatt tttttgacta catcattgta attgccatta
     3781 tatagcgaaa tttacacgat taaagagaca cgtctacatg tagacgtgtc tacaaatcta
     3841 caaatctact ttttagattt tcgctggtaa acctctagta aagtctttat ttctcttcgt
     3901 tcatccgtcg ttaaagaagc ctcataatta tctaggagga tttctatcag ttcattaaca
     3961 ctatctaaat tgaaagaagt aacgattgcg ttcagacgat gtgaagtatc cgttgcacaa
     4021 cgaatagtag ttgtcgtgcc cctttctttt tttcgaggga cgtctttttt ttggtctcca
     4081 ttagtttgct gagattcgtt tactgtaaat aaactggatc gattaaaaga ttgttggggt
     4141 ttcacttctt cttttcgctc aagaagattc cgtttccctt tatttttatt taataaatta
     4201 tctgccatac tatcaccctc ttctttcttg tatatggtat gaataatgtc tattgtttgt
     4261 ttcgagattc tatctcttct aatcgtttca acatctcttc agtgacacgc ttataaagcc
     4321 ccattactct cttatcgtga atatcatatt tagtgtctaa atcaggatca actatcccaa
     4381 taatgtcata tcgttttaat cgttccatat ttttaactac attttcgaag agattttctt
     4441 caccaaattt ttctttcgca gtattgagcg tagcttcgtc gacctttgaa ttgtttttta
     4501 gtaatacggg tagtacacct aaaatatcaa aatccgcatt atagttatca atgagttctt
     4561 gcaagtaccc tacataagct tcggccccta ccaaagaacg ttcttgcgtc tgtaggacaa
     4621 taatggtgta gtcggaagct aacaaagccg agtccgtata agtacttaaa gtaggtggaa
     4681 catcgaccaa aataaaatcg tagtcatcct tgatcttagt cattaattca tttaaaaaag
     4741 tcaccctatt aaattgactg tctggaaatt tcttttctaa aaataaggga taagaggtaa
     4801 aatctgcaaa gctaggcaat aaaaaaagat tttctttgat ttcaataata atgtccgata
     4861 aatcttcatc tgctatggca gacattagcg tcttgtcaaa agtaacaata ttattcgtaa
     4921 ttcgctgttt cgtatgtaaa tacaaagatg ttgcattcgc ttgaggatct aaatccgcta
     4981 ataatgtctt atagcccagt ttagataatg tgtaagccat catggcacta ttagttgttt
     5041 tacctgttcc acccttaaaa tttccgaatg ttataacttt agccattgta accctctttt
     5101 cttagttgtc tacaaatcta catttgatat tataacgtaa atataccatg ctaaatcaat
     5161 atgtcaaacg ttatcaagaa aaaccgtcta caaatataca tgtagacgca tatacattaa
     5221 aaatcttgat aacaaaagga tatcaagcat catgtatatg catctacaaa tctacaaatc
     5281 tacatgtaga cgtgtataca aatctacaaa tctacatgta gacgcatcta cagaaaaacg
     5341 agattttgcc tacaaattaa ggttttacaa aaaaaaaata gtgtagtaca tttgatttaa
     5401 ttcaaagcaa aactaaaatt gaatacaaaa aaagccccgt agacgccaat ctacgagaca
     5461 cgcaaaactt atc
//
LOCUS       DACDNO010000115         5245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDNO010000115 DACDNO010000000
VERSION     DACDNO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.117"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..357
                     /locus_tag="IWI22_002715"
     CDS             1..357
                     /locus_tag="IWI22_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170037.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            373..723
                     /locus_tag="IWI22_002716"
     CDS             373..723
                     /locus_tag="IWI22_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP9170038.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            752..1531
                     /locus_tag="IWI22_002717"
     CDS             752..1531
                     /locus_tag="IWI22_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP9170039.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1542..2219
                     /locus_tag="IWI22_002718"
     CDS             1542..2219
                     /locus_tag="IWI22_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP9170040.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2238..3344
                     /locus_tag="IWI22_002719"
     CDS             2238..3344
                     /locus_tag="IWI22_002719"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP9170041.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3334..4425
                     /locus_tag="IWI22_002720"
     CDS             3334..4425
                     /locus_tag="IWI22_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP9170042.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4438..5178
                     /locus_tag="IWI22_002721"
     CDS             4438..5178
                     /locus_tag="IWI22_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP9170043.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
BASE COUNT         1679 a          861 c         1251 g         1454 t
ORIGIN      
        1 atgattaaag tagttattgc agaccgtatg ggtaaaggac aaaatgtggc caaaggggta
       61 gaagcagctg gtggaaaagc agttgtcgta ccaggaatgg gggcagatat gcgtttgggc
      121 gatgtgatgc aacaagagca tgcagatatg ggtatctctt tttgtggtag tggtggtgca
      181 ggtgcattaa cagcagcaac aaaatatggc tatccagaac gacatggtat gcgttcgatt
      241 gaagaaggga tcacagctat caaggatggc aaaactgttc taggtttcgg ctttatggat
      301 caagaggaat tagggaaacg tctgacagaa gaatttatca aaaagcatgg aaaatagaaa
      361 gagggacatg aaatgagcgt tcaaaagaca attcagcaaa aagttatcgt ctccggtaaa
      421 ggggaaacaa agcaacatgc ttttgcatca gcgttagcag aaattcaaaa caaaataatg
      481 gaacaatcaa acgtattatt gcgaatcgaa ccagtcaatg tggatatttt aaaggcggtc
      541 gaaaacaaat acacagaacg ctttctattt ttcttcttcc cacggcaacg tattagctat
      601 gaagtcgaac tctctgttac tgtggctgta acagaaataa aattggcaga catatcgttc
      661 agccagcaaa tgactgaaga tccaaatggg attgccattc catttgtaac taaaaaaatc
      721 taattttgaa tcataagggg gaaagaataa cgtggatttc gtaatcattc tagtcaaatc
      781 ggtcattatc gggggattac ttggttttgc agcatctgtc ggggcagctc gaatgtttca
      841 tgcacctaat acacaaggtt taggagcttt tagaacatta ggcgaaatga atgcctgcat
      901 gggtgatcca gcttctcatt tttcatttgg tttaggcttt ttctttaatg catgggcttc
      961 ttcagttggt gctggtgcat tcacacaaga tgtgacgcat cgaatcattc ccaattgggc
     1021 agcggcagca cttctagcaa aaaacaaaga tgtcacccag accatgcatg atccgaaaaa
     1081 aatggggatc attggagcgg ttatcggggc agtggtggta gccttcttaa acacaacagc
     1141 tgcagcaatt ccagaggcgt tacaagtgac ggcggtttcc gttttggtac cagcagcgac
     1201 gattctgatc aatacagtaa tgccagttat tttttggttg gcagctttag acgcagggaa
     1261 acgaactggt ttttggggca ctctttttgg tggtatcgcg caattgatta tgggaaatgc
     1321 ggtacctggt gttgttctag gaatcttgat tggtaaaggc gtcgatgaga gcggttggaa
     1381 tcgcgtcacg aagagcatga tgatcgctat tgttttatta tttattttca gtggattttt
     1441 ccgtggattt gatatggaaa tgatcaagag cttccagctt caagtgccta attggcttga
     1501 taacttgcac aacatcttta gtgtgaaata ggaggagaat catggagaca acagaagaac
     1561 aagcaaaagt attagaagca aagcggcaaa agagcttttg gtttgcagac tggtcttttc
     1621 cgctaatcgt aggaattatg tccgctgctg tatttgcagg aacacacatg tatgtgatgt
     1681 atggtgtggg tgcattcaat gaagtttcga tcgtggcgat gttaaaagcc gggctggatg
     1741 gcggatccta tggcgcagcg gcagcgtttg gtgctagttt cttatttgcc agaattttag
     1801 aaggctcttt agttgggatc ttggacttag gcggttcgat cctaactggg atcggaattg
     1861 gggtaccagc aattctctta agtatgaaaa tagcggcacc aatcgataat tttgcactct
     1921 ctttattgac tggtttggtt ctaggtttga ttgttggtgg catcattgtc ttgattcgta
     1981 aatttacgat caatcaatca aattccactt ttggtgctga cgtcatgatg ggggcaggaa
     2041 attcatctgg acgttttttg ggtccattaa ttatattaag cgcgtgtgca gcatctattc
     2101 caatcggcat tggcgctaca ttaggagcac taggattcta tgcttggaaa aaaccaattg
     2161 ctggaggtgc tattttagga gcaatgattt taggagcaat cttcccggca gtaaattaat
     2221 agaaagaggg tcattaaatg tttgatactt tgattaaaaa tggaaggtta gttgatggat
     2281 ctaaaataga ggtcgctata gaaaaaggca tgattaaagc agttgcagca gaaataaatc
     2341 agccggcgca agaagtagtt gacttggagg gaaagtatta tttatccgct gggtggatcg
     2401 atgatcatgt ccactgctat gaaaagatga atctttatta tgactatcct gatcagatag
     2461 gggtcgaaaa aggtgtgacg acggtcattg atgctggaac tacgggagca gaaaacatcc
     2521 gtgaatttta tcaattgacg aaatcggtca agacaaatgt gtatgcgcta atgaatatct
     2581 ctaaatgggg cattgtcaaa caagatgaac ttgctgatct aacaaagatt caagaagatc
     2641 tagtaagtac tgcgttagca gaactgccgg aatttattgt cgggattaaa gcgagaatga
     2701 gtaagacagt tgttggtgaa aatggcatta agccattaga attagctaaa aaaatccaga
     2761 cagaaaacaa tgatctgcca ttgatggttc atatcggttc tgcacctcct gagttagaag
     2821 aagtattgtc aagaatggaa agaggcgatg tattgaccca ttgttttaat ggaaaaccaa
     2881 acggtatttt agatccaaag acagatcaaa ttaaaaactt tgtctgggat gcttacaata
     2941 atggtgtcgt ctttgatatt ggacatggga cggatagctt taatttccat gtggcagaaa
     3001 ccgcactaaa agcaggaatg aaagctacct ctattagtac ggatatctac atccgcaatc
     3061 gtacaaatgg cccagttttt gatctagcaa ctactttaga aaaattgagg gtagtcggct
     3121 attcttggga agaaatcatt gaaaaagtta cagcagttcc tgcacggaat tttcatttga
     3181 agaataaggg ccagttagct gtcaattatg atgcagattt gacgatattt gagattgttg
     3241 aaaatgaaaa ggaattgaaa gattccaacg gatttacacg tatagctaaa gaacaaattt
     3301 taccgattaa aacaattatt ggaggcgtca tatatgacaa ttagctatgg aaagtttgat
     3361 ttaaaagaag tcattaatgc atcgggaaaa atgacgatcc taggggtttc taaggtatct
     3421 gaaagtgtcc tagcagcaca acgatttgga ggcgagcatt tttttgagat gagtgatctt
     3481 gccatcaaaa ccggcgctta tctttctcaa ttactgaaag tggaagatgc gcaaatcgtt
     3541 tcttctgctt cagcagggat tgctcaatct gttgctgctt taatcggaca gggcgacatg
     3601 taccatgtct atcatcccta tacagaaaaa atcgctagac gggaaatcat catacctaag
     3661 ggacataatg tagattatgg gacaccggtt gaagtaatgg ttgctcaagg tggaggaaaa
     3721 gtcatagaag ctggctatgc aaatatgtgt acaccggaac atgtttccat gatgattacg
     3781 gaaaaaactg ctgcgcttct atatattaaa agtcatcatg cagttcagaa aagtatgctg
     3841 acggtggcag aaatggtaga agttgccaaa gctcatcgtt tgccattgat cgttgatgca
     3901 gctgcggaag aagatttatt caaatatagc gaaatgggcg ttgacttagt gatttactct
     3961 ggggcaaaag caatcgaagg tccgagtgcc ggactggtca taggaaaaaa gaagtatatt
     4021 caatggattc ggctacagtc taaaggtatt ggccgagcaa tgaaaatcgg taaagataat
     4081 attttaggct ttacccaggc actagaagat tatttaaaaa atgggagcga atccggtgac
     4141 tccatgaagc aacgattagc gccttttgtt aacgcaatca atcaacttcc tgacctttca
     4201 gcgaaaatag ttcaagatgg tgccggcaga gatatttatc gagcaagtgt aacgattaat
     4261 ggcgataaaa gtgcgaaaga agtcattcag gaattaaaag cacgaaatcc agcagtctac
     4321 acccgagaat atcaagcaaa taacggaatc attgagttcg acattcgttc cgtcagccaa
     4381 aacgaaatgg aaaaaattat aaaaagattg agagaaatca tgtaggaggc ttttaaattg
     4441 tcagttatcc caaattatta taaaaatcgt gtatgtttaa atgtattagc tcattcagtt
     4501 gaaaatgcaa aagcatgcta tgaagcagcg gaagggcatg tcgtactagg tgtgctatcg
     4561 aaaaattatg ctagcgatga agcagcaatc gaagatatga aaaggtacca agacgcaacc
     4621 aataatgcgt tgtcagttgg attaggcgct ggagatccaa accaaagtca gatggtttcg
     4681 cgtttatctg gggttttaca accacaacat gtcaatcaag tatttactgg cgtggggact
     4741 tctcgtgctt tattgggcca aaatgataca gtcatcaatg gattggtttc accaacaggt
     4801 aagatcggct atgtcaatat tgctacaggt ccgttaagtt cacaagcgcc agcagctgaa
     4861 gtgcctattg aaactgccat tcaaatgctg caagacatgg gaggtacttc aatcaagtat
     4921 ttcccgatga aaggcttagc acatagggaa gaatttaaag cagttgcgga agcttgcgca
     4981 aaatatgaat tctatttaga gccaacagga ggaattgatc tttcaaactt tgaagaaatt
     5041 ctgaaaattg caatcgatgc aggtgtaaaa aaagtgattc ctcatgttta tagttcaatt
     5101 atagataaag aaacaggaga tacgcgtata ggagacgtaa agatactatt aagcatggta
     5161 aaaaaaatta ttcactaact cattagaaaa taagaagctt attccagaat gaaattaaat
     5221 ggaataagct ttttataatt aatta
//
LOCUS       DACDNO010000116         5082 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDNO010000116 DACDNO010000000
VERSION     DACDNO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.118"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            98..778
                     /locus_tag="IWI22_002722"
     CDS             98..778
                     /locus_tag="IWI22_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9170044.1"
                     /translation="MDHFKGKRFQKDVIIVAVGYYLRYNLSYRDVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYTIWKKKNKQSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEI
                     KVLLGIQA"
     gene            complement(848..1396)
                     /locus_tag="IWI22_002723"
     CDS             complement(848..1396)
                     /locus_tag="IWI22_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170045.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1397..2254)
                     /locus_tag="IWI22_002724"
     CDS             complement(1397..2254)
                     /locus_tag="IWI22_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9170046.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2540..2827)
                     /locus_tag="IWI22_002725"
     CDS             complement(2540..2827)
                     /locus_tag="IWI22_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9170047.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKTLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2817..3146)
                     /locus_tag="IWI22_002726"
     CDS             complement(2817..3146)
                     /locus_tag="IWI22_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9170048.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(3223..3495)
                     /locus_tag="IWI22_002727"
     CDS             complement(3223..3495)
                     /locus_tag="IWI22_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9170049.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3562..4242)
                     /locus_tag="IWI22_002728"
                     /pseudo
     CDS             complement(3562..4242)
                     /locus_tag="IWI22_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(4663..>5082)
                     /locus_tag="IWI22_002729"
     CDS             complement(4663..>5082)
                     /locus_tag="IWI22_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9170050.1"
                     /translation="VITMEDCEQVNLKLDMHMVKTLFLCNRQKTNYYLFITSGDKPFK
                     SKDFSKALGISRVSFASKEDMTSMLQTKIGAATVFSTLIDTKNKIKIIFDKDVTDDRW
                     YGCSDGTTTGYLKISTNEIIAKLLPYTKHQMEIVKIS"
BASE COUNT         1517 a          968 c          770 g         1827 t
ORIGIN      
        1 ggaatcaacc cataaatctg tcggttctgt tgcaaagttt tctgataaac tgattttaag
       61 gtaaaatgaa tggaaaagat agcttggagg aatcaagatg gatcatttta aaggcaagcg
      121 atttcagaaa gatgtgatta ttgtcgccgt aggctactac cttcggtata atcttagcta
      181 tcgtgacgtc caagaaatcc tatatgaccg tggcattaac gtttctcaca ccaccatcta
      241 ccgctgggta caggaatatg gaaaactact ttacactatt tggaaaaaga aaaataaaca
      301 atccttctat tcgtggaaga tggatgagac ctatattaaa atcaaaggga aatggcacta
      361 tctttatcga gcaatcgatg cagatggttt aaccttggat atttggttac gtaaaaaacg
      421 ggacacacaa gcagcctatg cttttcttaa gcggttagtg aagcagtttg atgaaccgaa
      481 ggttgtagtc acagataaag ccccctctat tacaagtgcc tttaagaaac taaaagaata
      541 cggcttttat caagggacag aacatcgtac cattaaatac ctgaataatt tgattgaaca
      601 agaccatcgt ccagtaaaga gacgcaataa attctatcga agtttacgca ctgcctctac
      661 cacgattaaa ggcatggaag ccatccgagg attatataag aaaacccgaa aagaaggcac
      721 tctcttcggg ttttcggtct gtactgaaat caaggtatta ttgggaatcc aagcttaaat
      781 catagatacc gtaagggatt ttattcttta tttaaaactt tgcaacagaa ccgttcttct
      841 ttgattatta tctaacttta ttattcattc cccacccttt gcgcagtgag tcttcggtat
      901 cttgttttat ctgactggta gttcttatta tttctccacg atcatctaat tctggaacaa
      961 ttggatattt ttctctgaac aagtctagtt ctttcaccac gtcattaaac aaatcttgct
     1021 tatcttcttg cgtaagattc tgtttaatat actcatctaa agcttttcta agcaattcat
     1081 ctcttgtcat attagtctca tacttttcta ctaaaactct ggaagtcgca tttaacaaag
     1141 tcgacagttt taaaggatgc ttggtggaat aaaccttatc aaactttctg attggtgcgc
     1201 caactttttt tgttcttcct ctactctgtc tttcaacatt ggaggaagtt tttatttctt
     1261 gaacttggtg cacttctaat tgcttatttt cagatggtgt aattgcttgc tgctttttct
     1321 ttgcttctgc ttcttttttt attttggcga gtcctttgtt gttcccaaaa atatcaagtc
     1381 ctttgatgtc agccaattac tccacctccg cataccaatt ctcgtgttct tccaattctt
     1441 tcaaaatact gataaataat tcgtggcttt tgcgatccca ttgttttaag agacctttct
     1501 ttgtcttctc catcgtaata cctgatccgt cgtatcgttt taagcgcgca tttttgagta
     1561 caacattttt aatcaaatgt tccccataaa tctcttgggc ttgtttatac atttcctgat
     1621 ctactgcgtc accgctttcc aacatgaaag gaataacacc agcaacatat aaatttgtat
     1681 caaattcttt cacaaaaaag tccatatact caagatattg catcgcattt ctcatcgatt
     1741 ttacttgggt ctgtaagacg acgattgccc agtccgcaat gtacatcgcg gatttactat
     1801 atacagaaat agaaggtggg acatctaaat agatgacgtc aaactcatca aataatggtt
     1861 ctagcttttc tttcatatat ttgaattggg acaatgaatc attatattgg tctttgatcc
     1921 aatcatcata catttcaaag gacatgtctg ctggaagtat atagaggtta ttgattactt
     1981 tatagacagc ctctttaggt ggaagattgt ttatcaacac gtcagtcaca gtattttcgt
     2041 aaacaatcgg ttcaccttct ccgcctcctt cttcgttagt gaagttcata tgcgttaagt
     2101 tcatcacatc agaagtattt ccttgcatat ccaagtcaat tatcaaggtt ctttttcctt
     2161 cattaactgc tgattcaaac cctaacattt gcgcgcacgt acttttacct acgccacctt
     2221 taaaattacc gattacatat ttcttcgcta ccacgttcct acctcttttc ttttagcaat
     2281 attcatttga tactattata acatatttat ttttagttac taccaaaaaa aagatatata
     2341 tgtactacct tataattaat aagtaaaagg tagtacataa ataaggaaaa gctgaaatat
     2401 attgatttat agctttcctc aaagtaatga cgttatttaa tttatcgaga agcagagaat
     2461 actcgcgact ttagtcatgt agatgaatca gatcttcgat aaaaaagaag ccgtccttct
     2521 taagaacgac aacaaatcac tagattaatt ttttcaactg ttcataaaag ttttcatgcg
     2581 ttccacataa caatactgga ataaccttgt tttctataat ctcgtaagct actctatatg
     2641 ttgtgcccgc atatttgaac ccactagacc aaatacctga taaatcccca gttttttgtt
     2701 cacccttata tggatcaatc gcaatttcat cataaattaa attcaaaaat ttttctttca
     2761 atgtcttctc ttttagcttt tttaaatact taatgacatg ttttgaagga acgatttcat
     2821 aagccaattt ctgttgccaa ctcctcttta gtcattactt tagaattagt aagtgtatct
     2881 tcagcaatat ctctaaagga agaatgcatt ttgttttttc tattcttaaa ttctacaaga
     2941 atctcatctt tatcgtagcc ttctgcaatt atatcagaca aaatatcttc gctaaaatca
     3001 taaaattctt tttgtggttc aacaaattca taaagaatac cattctctac caactttgct
     3061 tcaacttcga ctccattagg aacattaaaa tctttaggta ctgtcaacat gattgaattg
     3121 ccttgttttc ttgtttttgt aatcatttaa atccctcatt tctaacagta tgtaattact
     3181 tagtaaatac attataccac gagataatgt atttacaagt aattactgtg actcgtaatg
     3241 cgtccttgct gaataaattt caaccacgtg ttcatcttca tctaccttat aaacaacccg
     3301 gtgttgtcta ttcaatcttc tagaatacct accttcatgt gtaggtttta acttctcaaa
     3361 tgattgggta gggagatagg gatcttcctt taaagtctga ataaccttct caaattgagg
     3421 tttcaaatta gactgtttga ttttctttaa atcagcttta gccgagtttt taatcatcac
     3481 agaatagtta ttcattataa gttatccggt tctgttgcga agtttgaaaa tcaaacgcgc
     3541 cctctctgaa ctccaaatat cttaggctgt tattcccatt aataccttga tttcagtaga
     3601 caccgaaaag tcgaagagcg ttccatttct tcggttcttt ttatatattc ctcgaatggt
     3661 ctccatgccc ttaatcgtgg aagaggctgt acggagactt tgataaaatt tatttcgtcg
     3721 tttaataggt cgatggtctt gttctattaa attgttaaga tacttcacag ttcggtgctc
     3781 tgtcttagta tataaaccca cactctgtaa ctttctaaag gcggagccaa gagaaggtgc
     3841 tttatcggtc acaattgctt tcggctcacc aaactgttta tggagtcgtt ttaagaaagc
     3901 ataggctgct tgcgtatccc gtttctttcg taaccagata tctaaggtta agccgtccgc
     3961 atcaattgca cgataaagat aatgccaacg tcccttaatt ttgatatagg tttcgtccat
     4021 tttccatgaa tagaaggatt gtctattttt cttcttccaa agataataga ggactttgct
     4081 gtactcttat acccaacgat aaatcgtagt atgacaaaca tttattccac gatcatataa
     4141 caattcctga acttcacgat agcttagatt gtaacgcagg tagtaaccaa cagcaacaat
     4201 aatgacgtct tttttgaatt gtttgccttt aaaatgattc attactctgt cctctctgtc
     4261 ttttttctca attttacact aaaatagatt ttttggaaaa ctttgcaaca gaaccctttt
     4321 attgaaagta agaagaacag gcttttttat tagaggcgtt caaaattgtt tgagcggtta
     4381 gcttttattt tttagaaata atttcaaaag tatttttgtt gtgtaaagaa agttgtgcca
     4441 accactttat ttttaatgtt tcaggatatt tatggaaatt tagcttattt gcaccaatca
     4501 caatggctcc gaatgaaacc agtattatct catagccaag attttgaata tcggttaaaa
     4561 catcatccat tttatcgaaa gattagatat tatttttacc aattgtattt gttaaagttg
     4621 tatttccaac tttaattaca atcctgtctc tatgaatgtt catcaggata tttttacaat
     4681 ttccatttga tgttttgtat agggtaataa ctttgctatt atttcatttg ttgaaatctt
     4741 caaatatcca gtagtagtcc catcactaca accataccac ctgtcatccg ttacatcttt
     4801 atcaaatatg attttaattt tattctttgt atcaattagt gtgctaaaaa ctgtagctgc
     4861 tccaattttg gtttgtagca tacttgtcat atcttctttg gaagcaaaag acactcttga
     4921 gattcctaaa gctttactaa aatctttaga tttaaatggt ttgtcgccac tcgtaataaa
     4981 taaatagtaa tttgtttttt gtctattaca caaaaagagt gtttttacca tatgcatatc
     5041 taatttcaaa ttcacctgtt cgcaatcttc catagttatc ac
//
LOCUS       DACDNO010000117         5066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDNO010000117 DACDNO010000000
VERSION     DACDNO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.119"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..272)
                     /locus_tag="IWI22_002730"
     CDS             complement(<1..272)
                     /locus_tag="IWI22_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9170051.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
     gene            complement(244..1443)
                     /locus_tag="IWI22_002731"
     CDS             complement(244..1443)
                     /locus_tag="IWI22_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9170052.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1573..2949)
                     /locus_tag="IWI22_002732"
     CDS             complement(1573..2949)
                     /locus_tag="IWI22_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9170053.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2949..3605)
                     /locus_tag="IWI22_002733"
     CDS             complement(2949..3605)
                     /locus_tag="IWI22_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9170054.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3615..4892)
                     /locus_tag="IWI22_002734"
     CDS             complement(3615..4892)
                     /locus_tag="IWI22_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9170055.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4911..>5066)
                     /locus_tag="IWI22_002735"
     CDS             complement(4911..>5066)
                     /locus_tag="IWI22_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP9170056.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1589 a          925 c          631 g         1921 t
ORIGIN      
        1 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
       61 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      121 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      181 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      241 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      301 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      361 gaacgctcct ttttttgtga cttttctgaa gctggagtgg acactttcaa ccgcattggt
      421 agtgtacata atttttcgaa tggcactacc ataatcaaat agttgttcaa catgagaaaa
      481 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      541 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      601 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      661 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      721 accgtccatc gaaatgaaaa aaacatcttc tacaccacgt actttcagtt catcaaatac
      781 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      841 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      901 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      961 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1021 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1081 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1141 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1201 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1261 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1321 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1381 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1441 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1501 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1681 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1741 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1801 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1861 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1981 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     2041 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     2101 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     2161 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     2221 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2281 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2341 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2401 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2461 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2521 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2581 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2641 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2701 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2761 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
     2821 agctctgtcc gtagcatctt taatagataa actaatgaga cttaaagttg acatagataa
     2881 aataactaat atgataatta atgattttaa actttttcta gttacataag caaatgcatt
     2941 ttttatcatt attcaacctc caaattcatt ttatttactt ttttcaactt tttaccactt
     3001 agttctaaaa tgatatccgc agaatctgct acttccttac tatgtgttac aactattaca
     3061 catttatttc tatctttagc taatgtcttt aatatattaa ttatttctcc agcagtaaca
     3121 ctgtctagat taccagtagg ctcatcagct agtattattg gagcatctga taccaatgct
     3181 ctagcaatag ctactctttg ttgctgtcca ccagataatt tcataacatt tctttttatt
     3241 tgttttttat ctaaacctag ttcaaacaag atactttcat ctgctgattt atttactaat
     3301 ctaatatttt caattggtga taaataatct attaaattat agttttgaaa tactaaagat
     3361 atattatttt ttctatgatt actatatcct ttattttgta tatcttcatt cttaaacaat
     3421 atttttcctg tttgaggttt atcaagtcca gcaagtaagg aaagaagtgt agattttcct
     3481 gttcctgact ttcctactat cgcataaaac ttcccaagtt caaatttttg atttactcct
     3541 gacaaaactt tttctttaga atttgcataa ctatatgcta cattctttat ttctaatata
     3601 tccattattt tctcctaact tatttttgat aagatttctt taggcttctt gattaacatt
     3661 aatgaagaag caaatacaac tgataaaaca ataatactta ttaatattaa ataactttgt
     3721 ccaagtgttg ttatatttga cataaaacta ctattattta ttaaacttcc accggaaatc
     3781 attgaatcct ctgagttaat gaatccatct acaattactt ttagtaatac atttcctaaa
     3841 aataaggaag atattatact tggtatcgat atgaatatta actcgaatat aaattgcatt
     3901 ataatttgta tcttagatgt tccaatagat aagaatatac ctatttcata aattctttcc
     3961 cttaaccata gaatcaagat taatgaaaga acaaccatcc cacctaacat aatagaataa
     4021 gtcattactt ttattatata ttttattcca ctcactgact ctaaagactc ttcaaacgca
     4081 ttagaatctt tttcaacaaa atactttgac tcatcaattt taagctcttt caatttgttt
     4141 aaggctaagt ctgtagattc tgcactacca gaatacatta aaattttatt tgcaatttca
     4201 ttattttctg aattgtttaa tatctcttgg cttgttgaat aatctacaaa caccatattt
     4261 tcactaaaat cagaagataa tcctgtatat gtttcctgtt ttttaccaga aaagatccct
     4321 ataattttaa atttatgact ttttattttt ccactttttt caatatctag taattcaaga
     4381 tcaacttcat cacctaattt tagattgttt tgtttagcaa attcttcatg aacaataatt
     4441 gaattcttat cattttcttc tatattttta ccttctttaa ttgtaaatac tccactacta
     4501 aataaaatat ttcttttagt attatttgta gcttcgagtg aaacaacatt cttaaattcg
     4561 tcagataaat cttctctatt tattctttgt tctccgctaa ctactttagc atcttttagt
     4621 tttgctaatc catcatattg aattattttt tcttcaattt cttttaattt ttcaatatct
     4681 ttaaattgat taacattaaa atatttacca tctttttttg ttattgatat tgaagaatta
     4741 gaactttcat ataaagcctt ttctatttca ttgcttgatt tcattattgt taaacaagaa
     4801 tacaagcaag aaagaactat agttaatatg ataaaaatta ttaaagttct atttttcttt
     4861 cttgtaatat atgctatagc attttttatc actttcctaa ctactcctct ctattttctt
     4921 actccataac taattgcatc cactggacaa gcatttttac atcgtccaca tctgatacac
     4981 tccagatgat tgcaattctc aactgaatct ttattcattt ggcaaacttt tttacatttt
     5041 ccacaattaa tacacttatc cttatc
//
LOCUS       DACDNO010000118         5046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDNO010000118 DACDNO010000000
VERSION     DACDNO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.120"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(46..1512)
                     /locus_tag="IWI22_002736"
     CDS             complement(46..1512)
                     /locus_tag="IWI22_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9170057.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKTRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1899..2495
                     /locus_tag="IWI22_002737"
     CDS             1899..2495
                     /locus_tag="IWI22_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9170058.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2553..3035)
                     /locus_tag="IWI22_002738"
     CDS             complement(2553..3035)
                     /locus_tag="IWI22_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170059.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3141..3338)
                     /locus_tag="IWI22_002739"
     CDS             complement(3141..3338)
                     /locus_tag="IWI22_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170060.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3633..4391
                     /locus_tag="IWI22_002740"
     CDS             3633..4391
                     /locus_tag="IWI22_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9170061.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4451..4879
                     /locus_tag="IWI22_002741"
     CDS             4451..4879
                     /locus_tag="IWI22_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170062.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4925..4997
                     /locus_tag="IWI22_002742"
     tRNA            4925..4997
                     /locus_tag="IWI22_002742"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4958..4960,aa:Gln,seq:ctg)
BASE COUNT         1715 a          992 c          854 g         1485 t
ORIGIN      
        1 caggaactgc tgcaaataag caattcctgt ttttatgttc attgattaat gaatagagcc
       61 cattagtttt ttgattggtt ctttcacagt aaatagcaag ataccaccga cgattgctac
      121 tgcaccgata ataccgaagt aagcagattc tgttcccggt gtgtagaagc gtgcaatttg
      181 tgcattgatt gcttgtgaag aagcatcagc taagaaccaa atagcgattg tttgtgattc
      241 aaatgctcgt ggtgctaatt ttgttgtcac agacaaacca actggtgaaa gacacaattc
      301 agcaataacc acgatgaaga aacttcccaa caaccacaat ggacttactt ttccacttgt
      361 accataaagc atacccggca acatcaatag caagtacgat aatcctgcaa ataaaagtcc
      421 aagagagaat ttcactactg tagatggttg acgttcacca agtttcgtcc acatcgttac
      481 gaatactggt gtcagaataa agatgaaaat tggattcaat gattgatacc agcttgcttg
      541 gatcgttaca ccaaatagcg tagattgtgt acgttcacta gcaaataaag ctaagataga
      601 tgaaccttgt tcttgtaatg accagaaaat gattgctgca aggaataatg ggatataagc
      661 ccatactctt gttttttctt cagcagttac atcttttgaa gtcagcattt tgataaagta
      721 atacaacggt aagataatcc ccaatacact gattgtattg acaaaaaagt caattgtcag
      781 atgaccagtt agttgtgccc cgccgaatac tgcagcgata acaacaagtg cgatgatcaa
      841 gttacggatg aatcctttac gttcttcagt tgtcaaagga ttcggcggtg ttgtacccac
      901 tccgtctaat gtcttgcgtc cttggatgat gtattgtaat aatccaaaga acatcccaat
      961 agcagctaaa gagaacccta aatgatagtt atattcttga ccgattgtac caacgattag
     1021 tggtgctaat aatgccccta agttgacacc catgtaaaaa atcgaaaatc ctgcatctct
     1081 tcgcaagtca gtctttgaat aaagatgtcc gaccatgcca gaaacatttg gtttcaaaaa
     1141 ggcggttccg ataataatca atgccatcga aacaaacaat gtacttacac caaatggtaa
     1201 agccaagacg atgtggccaa gcataatgaa aactccaccg aagaatacag tgcgttttgc
     1261 tcctaacact cggtcagata cccatccgcc gacgatacta gacatgtaga tcaaagatcc
     1321 gtagatcgac atgatagcta atgctgttgc tcgtggtaat ccaagtcccc cgttggcaac
     1381 tgtatcgtac atataataga taagtaatgc acgcataccg taataactaa atcgttccca
     1441 catctcagtg aagaacaaag ttgacaatcc cttcggctgc ccgaagaagc ccttgtcttg
     1501 ctgtttttcc ataatctata ccccaattct ttaaattttc agaaaatatt atacatcttt
     1561 accgaaaaat ttaactaaaa aacttattta agaaatataa actaattttc tataaaagca
     1621 agtaattttt aatggcaata tcataaaaaa aagaagatag agatttaata attatatatc
     1681 agctgttttt ttcaaataag caaaaattcc taaaacacaa caatgaaagc gataactaag
     1741 aaaatcctta cataacgatt tttcttatta aaattgtttt ttgaacttcg cttatttttt
     1801 gtgagcatat ctgtttactt actttttgta aggtgctata cttcatttcg taatcaatta
     1861 aaaaaatgca aaacgcaaat caaaggagaa attttattat gacaactttt actgacactt
     1921 tgaaaaaccg ccgttcaatc tatcatctag gacgtaatgt ttcactttct aatgaagaat
     1981 taacaacttt gatcaaagaa gcaattaaag aaagcccaac tgctttcaat gctcaatcaa
     2041 cacgcgcagt tatccttttc ggagatgcac atgaaaaact ttgggaaatc actgaagaag
     2101 cacttcgccc attgactcca gcagaagctt tcccaaatac acaaaacaaa ttagctggtt
     2161 tcaaaaatgg ttatggtaca gtattattct tcaaagatac tgacgtggta aaaggtctac
     2221 aagaacaatt tgaattatat gcagacaact tcccagattg gtcagaacaa tcaaacggaa
     2281 tcgcaacagc taatacttgg gtagcattag ttgataaagg attgggcgca aacttgcaac
     2341 attacaaccc agttattgac gaagcagttg caaaagaatg gaatatccct tcaaactgga
     2401 aactacgttc gcaattagta ttcggttcac cagaaacacc ggctggggaa aaagaataca
     2461 tgaacgatgc cgatcgcttc cgcgtatttg gataatttta tttgaaataa aaaacagagg
     2521 ttgtgacaaa catcttgtcg caacctctgt ttttattctg gatcaatcat aatatcaatc
     2581 tctgcctcat cgtttaagcg ttgggacaaa aatggaacta attcttctaa tgatgcagaa
     2641 aatttcagca tcggtcctcg ccatccgttt cggaaaaaat tcatttttac gattttagaa
     2701 ccttttggct gatataatac aattttattg atctcagaaa aaggaacacc ttttttgtca
     2761 aagctattca aaacaagtcc ttcttctgtg attcctctac tgtccaacaa aaaactaata
     2821 aaaatcattg ctgacaagat cccttttgcc aattcatctg ctgtaggatg actgatgagt
     2881 gtataccaaa tgactagtga cactaagaag ggtatcacca agtagaccca tcgatttcgc
     2941 acagtccgat aaataatttc ttttcgaacc agccaaagaa ataaaaacat cacaacaacg
     3001 aataaaaaca atagaaccgt aaagaatgtt accatgacaa ctcgcctcgc tttttcttct
     3061 ccagaaaata ggaaaagaat actgatttac cttctcaaag aaattggact tgcagcacgc
     3121 cgcacgtcca attgttttct ttattcaaat gtatcatatt ttgcaacaac catgcttgct
     3181 ttttcatcta ataattcttg gtcctctgca gcacattggg caactttgac cacggcgcag
     3241 tttgtcattt ttgatacaac ttcaccaatg accgtttttg taaaaccaat tggtttgcat
     3301 gcatattgtt ctcctaaatt aatcatttct tccgtcatca aaatcctcct aaactgctgg
     3361 aacttttctc cacattacca ctgctatttc tttggagatt taactcattg atttttattt
     3421 acagttaaaa atcaacctta caggcattat agcgtactgt tttcgttttc acaattaaaa
     3481 cagctgttgt atttagaaga atttttttca aaaaattaaa acatattcct taaaaactaa
     3541 ttatttccta catattaaaa tataatactt ccatttccaa taaaaataca tttataatag
     3601 gaaagataaa cagctgaaaa gggagatttc aaatgaagaa aaaaacgttc aaaaaactat
     3661 tgtttctagg gggcacagct gctcttttat taagcggtac aagttattac ctacatatcc
     3721 attctccaga acatacaatg aacactgcac aaaaaagttc aacacaaaaa acgaaaaaga
     3781 caaaagagaa agcagaagtt ttactagatg ttccattaga gagccagttt gattctcctt
     3841 ctttagaaaa tggctgtgaa gtcacttcgc tttctatgct tttatctttt tatggttatg
     3901 aaacaactaa aaaccagtta gctgagcaat tggattacgt ccctgttttc aatgcagatg
     3961 ggactcatgg cgatcctaat gaaggatttg ttggtgatat cagtggcgga gactgggcta
     4021 tggtcgtcta tgttccccca gttgcctccc ttgctcagca aatcgtccaa acggattatc
     4081 acacttttcc aaaaaccgat gctcaactcg atgatatcaa aaaagcacta tctaaaggaa
     4141 atcctgtctg gacttctgtg actatcaatt ttgaagtccc agatgaaact gattttatga
     4201 cttggaccac aaataatgga gaagtaagag tcactcctct tgttcatgct tgtgtagtca
     4261 ctggatatga caaagaaaat atttatgtca acgatcccta tggtgtaaaa aatcgagcag
     4321 taccgatcaa cgatttttcg gcgattttca ctgcaatggg tggacagatg ctaactttgg
     4381 agaaaagcta gtaattcaaa caaaaatccc ttatactcaa gataccaaac ctttgaaggg
     4441 agcgtagata atggaaaaat tcacacataa aaaaatggat ccaaatgaaa ttccgattat
     4501 ttttgtccgt gactgcaagg gaaatgttca aggtaaagtc tctattaatg agtggaacga
     4561 gcgtcgcaga cctgctaccc tcaatgaact ggaaatcaaa ttgtatcgtc aatctcttgt
     4621 ttattacgca gatcaagagt acgagaaagc caccgattta ttgaaatttc taattgctcg
     4681 aacagagtat actcgctttg aatatattga acgtttagca aacatctatc atattatgaa
     4741 tgaacctgtc aaagaatatc agttattgga caccgtcttg tcagtagcag aactgattgc
     4801 ccttcctgct ggattagaaa agaaactagt acgccgcctt ttacgagtaa agcaacaact
     4861 atccgatcaa gaaaaataac ttgcgttttt ttaggaaagt gctatgatta ttttgttgaa
     4921 taaatgggat gtagccaaac tggtaaggca cttgactctg aatcaagcaa ttgtaggttc
     4981 gaaccctgcc atcccaattt gatgtgcttg ctgaaaaaat gaaccccctg taaaagaaca
     5041 atcttt
//
LOCUS       DACDNO010000119         4862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDNO010000119 DACDNO010000000
VERSION     DACDNO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.121"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1219)
                     /locus_tag="IWI22_002743"
     CDS             complement(<1..1219)
                     /locus_tag="IWI22_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9170063.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEM"
     gene            1280..1855
                     /locus_tag="IWI22_002744"
                     /pseudo
     CDS             1280..1855
                     /locus_tag="IWI22_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2036..3229)
                     /locus_tag="IWI22_002745"
     CDS             complement(2036..3229)
                     /locus_tag="IWI22_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9170064.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3313..3516)
                     /locus_tag="IWI22_002746"
     CDS             complement(3313..3516)
                     /locus_tag="IWI22_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP9170065.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            3773..4015
                     /locus_tag="IWI22_002747"
     CDS             3773..4015
                     /locus_tag="IWI22_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170066.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(3937..4170)
                     /locus_tag="IWI22_002748"
     CDS             complement(3937..4170)
                     /locus_tag="IWI22_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9170067.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4177..4608)
                     /locus_tag="IWI22_002749"
     CDS             complement(4177..4608)
                     /locus_tag="IWI22_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9170068.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1325 a         1110 c         1004 g         1423 t
ORIGIN      
        1 acatttctac aaaaaaatag tgattctgtt ttgaaaattc gatttttagt tctgcatttt
       61 cgtaaacatc caacattttt ttcgcactgt atagacctaa cccgccattt tttttagagg
      121 tttttcctct tttgaccatc tccgatagcg tcaaattcgt tggatttgcc ttgttcgcta
      181 tactaaaacg tgatccctct ttttcttcca gcagattgat cgtaatcgga ctctgatcgg
      241 ctacacgatg atcgaatgcg ttagaaataa aaatagataa acagcgaata aaaacaacca
      301 gatcaatcgg gattgttttc acttcgacag gaataactac ttggatcggg attttctccg
      361 attctgcttt ttctagaaat tttaaaaaga cacccctgac agctggggat acaatatttg
      421 ttagctgttg tatttgcggt ttttcaaaaa tagctgcaga atgatcttct aatatttcta
      481 aaaattccaa cgtttctttt ttattttcct gaagagtaat ttttaatgaa aacagtagac
      541 ttttataatc atgtttgaat tcctctagat gatggtaata ttcttcattt atttgataca
      601 gttgaagcga ttcctgatgt tgtctttctt ttacttggaa actgatcaac ataatggaaa
      661 aaaaaaaaga aaaaaaactg atgactatcg acaaaagaga tagaagcaga taattagatg
      721 agcggaagtg aacacttccc aataccaagt aaatcacgcc ccagcttaat aaaatacaaa
      781 tacctccaaa atagtgttca gttggataag taaaaagcaa gttgagaaat ctcgtctgtt
      841 tgtctatact ttttactaat agatagaaaa taaatgcgat aagtagtgca aaaaaacttg
      901 ctgttccaag atgtctcttt cctaaataat ccatcagatc aaaaaccaga aaaaagctga
      961 gtccagtgat agcaaaaaaa agattactga tcaaacagct gataaacagg tttttcgtag
     1021 taaaataaaa aagaaacatc aataataagc caactaggta aggaaagctc actttaggga
     1081 taggaagtaa aagtgtcacc accatcaaag ctatacaacc aaagctgttt tttactagca
     1141 gttgtttcga aaagaataaa tagataccta aatctaaaat caaagtaaag gtcaaataaa
     1201 gtgtcactgt tgcgaccatc ttattcctcc attgttttgt ttcaaaatgt acacatattt
     1261 ttttaatagg agcctgcata tgaaaaagta ttttatcata gaagataacc agtttcacag
     1321 cgaatggatc atcagagaat tggtcaatca tcatatcgag aatatcgtgc agctcacttc
     1381 tatctctcaa atcaatcagt tgatccagtt gggcactatt gataatcacg atgttttctt
     1441 tatcgatatc gacttgaagc aatcactcac aggtattgat gtcgccgaac aaatacgtaa
     1501 aaaaaatgca catagtaata ttgttttttt caccgcttat acgaattttg cagtagagat
     1561 catcaatcgg aaaatccttc cgattggata tatcagaaaa gacaggaatt tatctgaaca
     1621 aattcgtcaa gtgctagaac agatcaattg gaaagaacag cagatcgtaa atgtcgataa
     1681 aatctttatt gatcaaaaag gtggaagtct cgtcctgtat cctcgggaaa ttttatatat
     1741 tgaaagtatc aaaggtttga aaaaccaagt tatggtcaaa acaactacag aagataaatt
     1801 agtgaatact tctttaaaac aattgaagac agagttagaa aagtatgctt actttttctt
     1861 aaaaattata tgggatttta gattatcttt ttagaagttc ttgaatgtcg gtatagcccg
     1921 tgtttccggg cttttccggc atttttgata ttgttagata cttacaaatg ttcttattac
     1981 ctctcaccat ttccccctca aaagtagtaa atggagtagt aaaaccggcc ttgtgtcagg
     2041 ctgccagccg ttccatttct gcccttgcgg aatcgaatgt ggcgtgtgca taatagttca
     2101 gcgtcatggt gatattggaa tgtcccataa tgtactgcaa ggctttgggg ttcattcccg
     2161 cattggctaa ccgggtgcag aatgtatgac gcatagcatg gggcgtcgtc gttttcggca
     2221 acgcttcttc atggctcttg ttgtacttct tcacaagcct gccaaacatc ccgccataat
     2281 ctaccgccgt catgggatag ccgttctgtt tgagaaacag gaaattggca tacccgtcaa
     2341 taatgaacgg ctttgcacct ttgcggtttt ttatcactcg ctggaacgcc tgatacactt
     2401 cctcattcat ggggattttt ctgacgccgc tttgggtctt gggttcgtca atatagtaac
     2461 cgacgccggt atttctcaaa agctggtggc tcacgatgat tacccggttt tcaaaatcca
     2521 aatctttgtc cgtcagtccg cacagttccg aaatgcgaag ccctgtcccc agcagaataa
     2581 cgattgcgtc ataatgcttt tggtaaacct tatcaccttg aataaaggac agaaaacttt
     2641 cttcctgtgc tggtgtaaga ggcacctttg gttccgtatc gtcgtccaga acgtcactaa
     2701 gctggaagtc aaacgggttt tttcggatgc aatcgtcctg tatggcggtg taaaaagccg
     2761 ctttcaggga acgcttgtcg ttattgatgg tcttataaga aaggcccttt tctttcatcc
     2821 gaagcgccca ttcctttgcg tcggacagct tcacactgtc aatcgggcag ctccccagcc
     2881 gatcttgttc cagaatacgc atcagccgtt cccggcccag tttggtgttg tgcttcacat
     2941 tgccccggtg cctgatctgc ttggcgtaga gctggcagac ggtcattttc ttgccgatgg
     3001 tgtctatccc gtcgtcaagg tctttctgga tttctttctc cttttcccgg agtgaaatat
     3061 cctcccgttt acctgccggg gtcttatccg taggcactaa cttccatgag taaacaaact
     3121 gcggttttcc aaaggtatca acatatttgt aagcatatcg tccgtctttt ctctggctct
     3181 ctccagaccg caaaacgcga tttttgctgt ctcgtctttt ttctgacatc tcgtcatgct
     3241 cctttccatg acggaaagag ccgcatatat gcttataaat tattataaca tatctgcggc
     3301 cctttttcac tattagattg tgtccaaagt gtcaattatt ttctcaaact gcttgcgttt
     3361 gatctggatg cggttgccat tcataatgac ccagccagag gccggatttt cctcggccag
     3421 cttacgcaat ttgttttctc cgatacggaa atatttagac gcttcctcaa tcgtcagcgt
     3481 gtatttttcc caaataggca catcatggtt cataatgcag ccccctttca caaattcaaa
     3541 ggtttgtgtg atttaggggc atagacggac ggctgctggc acagctccac gggactttca
     3601 cccccgcgtg gttctcacgc ggccctaccc attgcctgcg acgcttttaa cgctcggact
     3661 gtggctataa gggagtatca tagtcctgcc tgtacgtcgt cgcccgcaag ccgccacgct
     3721 cacattgcgg cgtggtgttc ctcgctccgc ttttcccgta gggaaaagta tcatggcgca
     3781 ccagccgtcc ggctcggcac agacagaatg tgtccgtatg ccctatgatg cgccgccgtt
     3841 atcttgcggg catatttatc aaggtgcgat caggccaccg ggaaaacacc gaaacgggga
     3901 aagggtaagc cgtttcatgc tgccggggcc ggttcgtcag ttcgtgacga tagcgtggat
     3961 gagcttgatt tccagacggc gcttcatggc ctcgtccaga cacacatgcg gatagccgtt
     4021 ttcgtcatag agcgtccggg tacacagctt gttcatgtaa ccgctgtaat actgcaaaac
     4081 gcgctctacg gctccggcat ccccagcgcg ggcggcctcg atcaccgaaa ggggcaaaag
     4141 ctcaatgccc ttgtagttcg ggttcttcat ccgcgtctaa cctcccttcg gcatcaacgc
     4201 ctttagctgt ttccgcagct ccgataaggt atttgtcctg tgccgctgaa cggcactccg
     4261 ggacattccc acaaggccgc cgatctcacc atctgccata tccagaacac agtgcaggat
     4321 taaaatactc tgctccatgc tgggcagcgc ggcaaaggct tcggcaacaa gctcattgtt
     4381 gatatgtagg tcatagccat gcgacgaaaa cgtaaagctg tcgctggggt agtgatctac
     4441 tgtacagagc ttgtccaaat ctgcctgtga caaagagctt agtgacactt cacggtcacg
     4501 gcggcgcttc atttcagcca agtagctttt ggcctcgtta cgcagtaccg tcttgcaaaa
     4561 agcatcgaac atgcaccgcg cttcatagtc gcgggggata gcatccatct tctcaccccc
     4621 tttctgtggg ggacttaggt ggttctctcc ctttccgcta acaagacttt gacaaggccg
     4681 ccagctaccc gctaaatacc tcctttttga aaatttctca aaaaattttt ttggggacag
     4741 ccgggccgca aaagacgaca aaaatcgccc ggcaggtcgc aaacctaccg ggcgagaaaa
     4801 tgctttgcta tgctgctgat ttacatggta tagtacatac tcataaccgg aatagccctg
     4861 tg
//
LOCUS       DACDNO010000120         4834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDNO010000120 DACDNO010000000
VERSION     DACDNO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.122"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..303)
                     /locus_tag="IWI22_002750"
     CDS             complement(<1..303)
                     /locus_tag="IWI22_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9170069.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYARSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEK"
     gene            complement(413..967)
                     /locus_tag="IWI22_002751"
                     /pseudo
     CDS             complement(413..967)
                     /locus_tag="IWI22_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747752.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(964..2076)
                     /locus_tag="IWI22_002752"
     CDS             complement(964..2076)
                     /locus_tag="IWI22_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HAP9170070.1"
                     /translation="MKHISVLIKPASSLCNLRCSYCFYANVSSLREVRSFGKMKEEVT
                     EKMIENIYADLEDGDQLTLAFQGGEPTMAGLNYFRTVIRLVAQQQKQVQVHYAIQTNG
                     TMINEKWCAFLKEHDFLVGLSIDGHPLYHDLHRVDPKGRGTFQRVLQTKQLFDYYEID
                     YNVLCVLTNPLAKEAKKVFRFLKEQQIDFVQFIPCLDDLDAKKRNSYALTPKRFAGFY
                     HQLLQLWLQELAEGHYISIKLFDDLLNLLIKRQVTACGILGNCQVQYVIEADGSVYPC
                     DFYVLDEYRMGYIQEQTLRQLFEQDIAKTFLCGRPEMPKKCLSCPFQKMCRGGCKRMK
                     DAIYVDNTGYCGYQQLLQEFVPQINRILGLLQGVSV"
     gene            complement(2157..2861)
                     /locus_tag="IWI22_002753"
     CDS             complement(2157..2861)
                     /locus_tag="IWI22_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9170071.1"
                     /translation="MIKPKYLEIKQNLKQQIVTGQFHNGDRFYSESEIIKKYNVSSIT
                     AIRALKELVAEGYIVRYQGIGTFVSRARKEKIVKFSDIEIFSFDNDKTHVLSIKRENK
                     KQILDTLKLSTDSYYYQIERIREANEIPYIYHKSYLPEQYINSNYPEINYYNSIYKRL
                     KSDYNIDLNEENFRETNEILFPAPNSVCKQLKINESEPVVFQTKLSCLSENNKPIEYV
                     ESYKKWNFYKIQLLSN"
     gene            2981..4759
                     /locus_tag="IWI22_002754"
     CDS             2981..4759
                     /locus_tag="IWI22_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAP9170072.1"
                     /translation="MTTFEIKEEFLLKGKTFKILSGAIHYFRIPPCDWEHSLYNLKAL
                     GFNTVETYVPWNLHEPQKGEFHFEGILDLERFLTIAQDLGLYAIVRPSPYICAEWEFG
                     GFPSWLLREPIHIRRNEIAYLEHVADYYDVLMKRIVPHQLNNGGNILMIQIENEYGSF
                     GEEKEYLRAIRDLMIKRGVTVPFFTSDGPWRATLRAGSMIEDDILVTGNFGSKAKDNF
                     NSMKQFFKEYDKNWPLMCMEFWDGWFNRWKEPIIQRDPQELAEAVKEVLEQGSINLYM
                     FHGGTNFGFMNGCSARGVIDLPQITSYDYGAPLDEQGNPTEKYYALRKMIHDNYPEIK
                     QLDPVIKPTIEKKKISLTNKVSLFATLDTISQPVQSKYPKTMEELGQNTGYLLYRTMI
                     ERDAEEERLRVIDGRDRSQLFLNQELQATQYQTEIGEDIQVSMTEEINQVDILIENMG
                     RVNYGHKLFAETQKKGIRTGVMADLHFITNWQQYSLPLENCKTVDYSKGWQPQHPSFY
                     RYEFLLEDIGDCFIDLRKFGKGIVFVNQTNIGRFWEVGPTLSLYISSGFLKVGKNEIV
                     IFETEGTYEPEINLLKAPLYKEMKEG"
BASE COUNT         1564 a          833 c          924 g         1513 t
ORIGIN      
        1 tttttcgctt cgctgatatc tttcaaagat agtaggacta aacttaccgt ctctggttcg
       61 tggaacatga agttctaacg taccaattcg agttgtaaac gaacgagcgt agtaaccatt
      121 tctttgtgat tgccgttctt ctgaacgttc ataggcattt gcctgaatat attgatttcg
      181 ttgttcttcc atcagctgat taaacatgat agtcataagt gtttttgcgg tatcattcgc
      241 accgctttcg tttataattg cttgaatttc ttcgctttca aaagtaaaat gtacttgagt
      301 cattgatatc agtcctattc gagtatgttt tctgcaaaaa acattgtact cctaactgag
      361 atcaatggct tttttgatgc ttcactttta cacaattata tggacgtaat caaaaaatcg
      421 tacaaatcga atagaccgtg gcttctttga tgggtaaagc aaacatcagc atcagcaagg
      481 acacattgat cggtccaccg ccaatgccca gcagactcgc tagaaaacca aggactaggc
      541 cacagaagca ataccagaag ttatttttca gttggaatcc gggccagtcg tatttggtat
      601 aaaagaaagc aaacagcagt gtcacaacgg tcaaaaagat ctgaatcatt tgtacctcat
      661 cttcattctg aaataaaagc agcaggtgat caaaagcgac attgcccaag atgcctccta
      721 agacggcgcc gcctgaaacc caaaggacga tttgccaatg gattttacgt ccactagcca
      781 actgtcttgc ggtcgaaacc agcgacatgg taaacacggc tacggtagaa taaaacgaga
      841 tggctgcaac agaatgggca ccgataaaat caaaaatggg tttgatcaga acaccgcccc
      901 ccatgcctga aacggcacca accgtattcg ccaacacgat cactataaaa taaatgaacc
      961 caatcacaca cttaccccct gtaacaaacc taaaatccga ttaatctgcg gtacaaattc
     1021 ctgcaaaagt tgctgataac cacaatagcc tgtgttatct acatagatgg catctttcat
     1081 ccgtttgcag ccgccacgac acattttttg aaacgggcaa ctcagacatt tctttggcat
     1141 ttcggggcgt ccacacaaaa acgtttttgc aatgtcctgt tcaaataact gacgtaaagt
     1201 ctgttcttga atatacccca tgcgatactc atccagcaca taaaaatcac aaggatacac
     1261 actgccatct gcttcaatga catattgtac ctgacagttt cctaaaatcc cgcaggcagt
     1321 gacttgtcgc ttgatcaata gattcaaaag atcatcaaaa agcttgatac ttatataatg
     1381 cccctccgct aattcttgca gccataactg caaaagctgg tggtaaaatc ctgcaaaacg
     1441 ttttggtgtg agagcataac tatttctttt ttttgcatct agatcatcta aacaaggaat
     1501 aaactggaca aaatcaattt gttgctcttt tagaaagcga aagactttct tagcctcttt
     1561 tgccaacgga ttggtcaaga cacaaagtac attgtagtcg atctcatagt agtcaaacag
     1621 ctgttttgtt tgcagcactc gctgaaaagt tcctcttcct ttcggatcga cccgatgcag
     1681 atcatggtag agcggatggc catcgatcga gagaccgacc aaaaaatcat gttctttcaa
     1741 aaaagcacac catttttcat tgatcatggt cccattcgtt tgaatcgcat aatggacctg
     1801 tacctgcttt tgttgttgcg ctactaaccg gatcactgta cgaaaataat tgaggcccgc
     1861 catcgttggc tctccacctt gaaacgctaa tgttaattga tcgccgtctt ctaagtctgc
     1921 ataaatattt tcgatcattt tctctgtcac ttcttctttc attttgccaa acgagcggac
     1981 ttctcgcaag gaactgacat tggcataaaa gcagtagctg cagcgaagat tgcagaggga
     2041 agatgcgggt ttgatcaata ctgaaatatg tttcatagta agaacctcat tttttaacat
     2101 agtttaatta tgaaaaattg ctataacgaa caaagtaaaa tcaaattcag tagtagttaa
     2161 ttagatagga gttgaatctt atagaaattc cattttttat aactttcaac atattctata
     2221 ggtttattat tttcagataa gcaggataat ttggtttgaa aaacgacagg ctcagattca
     2281 tttattttta gttgtttaca tacactatta ggagcaggaa ataaaatttc gtttgtttcc
     2341 ctaaagtttt cttcgtttaa atcaatgttg tagtctgatt ttaaacgttt gtaaatagaa
     2401 ttgtaataat taatttctgg ataattagaa tttatatatt gttctggcaa ataagattta
     2461 tgatagatat aaggtatttc attagcttct ctaattcgtt cgatttgata ataatatgaa
     2521 tcagttgata attttaatgt atctaatatt tgttttttat tttcgcgttt aatcgaaaga
     2581 acgtgtgttt tatcattgtc aaatgaaaat atttctatat cagaaaattt tactattttt
     2641 tcttttcttg cacgtgaaac gaaagttcct attccttgat aacgtacgat atacccctcg
     2701 gcaactaatt cttttaacgc acgaattgct gttatagaac taacattata ttttttgata
     2761 atttctgatt ctgaatagaa tcggtcgcca ttatgaaatt gaccggttac aatttgttgt
     2821 tttagattct gtttgatttc tagatatttc ggttttatta taagctcccc ctttaaatta
     2881 ctcgcatcta tattaacata cttaaaaaaa aattgttgac ttttaataca aaagcattaa
     2941 tatactaagt atattaacac attgaggtga aaagtaacag atgactacat ttgaaataaa
     3001 ggaagaattt ttattaaagg gaaaaacatt taaaatacta tctggtgcaa ttcattattt
     3061 tcggattccc ccatgtgatt gggaacattc tttgtacaat ttaaaagcac taggatttaa
     3121 tacagtagag acatatgttc cttggaattt acatgagcct caaaaaggag agtttcattt
     3181 tgaagggata ttggatttag agagattttt gacaatcgct caagacttag gattatatgc
     3241 tattgtgcgg ccatcgcctt atatttgtgc tgaatgggaa tttggagggt ttccgtcatg
     3301 gctactccgt gaaccgattc atatccgaag gaatgaaatc gcttatttgg agcatgtagc
     3361 agattattat gatgttttga tgaaaaggat tgttccccat caacttaata atgggggaaa
     3421 tatattgatg attcaaattg aaaatgagta tggatctttt ggggaagaaa aggaatattt
     3481 gcgtgcgata agagatttga tgattaagcg aggagtgaca gttccttttt tcacttcaga
     3541 tggaccgtgg agggctactt tacgagcggg aagcatgatt gaagatgata ttttggttac
     3601 tggtaatttt ggttctaaag caaaagacaa cttcaactct atgaagcagt tttttaaaga
     3661 atatgataaa aattggccac tgatgtgtat ggaattttgg gatggttggt ttaatcgttg
     3721 gaaagaacca attattcaac gagatccaca ggaactcgct gaagcggtaa aagaggtatt
     3781 agaacaagga agcattaatt tatatatgtt tcatggagga acgaattttg ggtttatgaa
     3841 tggttgttca gcaagaggag tgattgattt accgcaaatt acttcctatg attatggtgc
     3901 accattagat gagcagggga atccaacaga aaaatattat gcattgcgga aaatgattca
     3961 tgataattat ccagagatta agcagctaga tcctgtcatt aaacccacga ttgaaaagaa
     4021 aaagatttct ttgacgaata aggtaagtct tttcgcgact ttagatacca ttagtcaacc
     4081 ggttcaatca aagtatccaa agacaatgga agagttggga caaaatacag gttatctact
     4141 ttaccgaaca atgatagaga gggatgcaga ggaagaacga ttacgggtaa ttgatggcag
     4201 agatcgcagc caattatttt tgaatcaaga attacaagct acccaatatc aaacagaaat
     4261 aggtgaagat atacaagtta gtatgacaga agaaatcaat caagtggata ttttgattga
     4321 aaatatggga cgtgtcaatt atggacataa actctttgcg gaaacgcaaa aaaaaggaat
     4381 tagaacggga gtaatggcag acttacattt tattacaaat tggcaacagt attcattgcc
     4441 tttggaaaat tgtaaaactg tggattattc aaaaggatgg cagccccaac acccaagttt
     4501 ttatcgatat gagtttcttt tagaggatat cggtgattgt tttattgatt taaggaaatt
     4561 tggtaaagga attgtctttg ttaatcagac caatataggt cgtttttggg aagtaggtcc
     4621 aacgctttca ctgtacatct ctagcggctt tctaaaagtt ggtaagaatg aaattgttat
     4681 ttttgagaca gaaggtacct atgaaccaga aatcaattta ttaaaagcac cattatataa
     4741 agaaatgaaa gagggatgaa caatgagtat tattggagta agaattgatg gacgtttaac
     4801 agaattaggc agaagtcgcc catcctctat aggt
//
LOCUS       DACDNO010000121         4772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDNO010000121 DACDNO010000000
VERSION     DACDNO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.123"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..369
                     /locus_tag="IWI22_002755"
     CDS             1..369
                     /locus_tag="IWI22_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9170073.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="IWI22_002756"
     CDS             549..1490
                     /locus_tag="IWI22_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9170074.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="IWI22_002757"
     CDS             1559..2290
                     /locus_tag="IWI22_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9170075.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="IWI22_002758"
     CDS             2434..4614
                     /locus_tag="IWI22_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9170076.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..>4772
                     /locus_tag="IWI22_002759"
     CDS             4634..>4772
                     /locus_tag="IWI22_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9170077.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HL"
BASE COUNT         1595 a          847 c         1067 g         1263 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt ag
//
LOCUS       DACDNO010000122         4714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDNO010000122 DACDNO010000000
VERSION     DACDNO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.124"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..1905)
                     /locus_tag="IWI22_002760"
     CDS             complement(1..1905)
                     /locus_tag="IWI22_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9170078.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTDMAPESALVQTEDGEFEEVDVDDVDEGDVLLVKTGAKVPVDGTVL
                     TGEGHINEASITGESVPVTKTEGSEVFAGTILENGTIQIWADKVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKWYTPTVLVLAFIVWAMTQNVELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVINDFSKVDTIVFDKTGTLTVGNPEVAETEVYGDNVE
                     KAYSYLASVETESDHPLAKAVLQHTGTVELFPVEDTDVVKGGGVVAQVDGHRIAVGNV
                     ALMEKENVQLSKKAQKDVKQFEKNGNSLVLTAVDGELKILMGIRDQIRPGVKQDLQEL
                     KNLGVKDLVVLSGDNQGTVDRVARELNLTEAHGHMLPEDKAAYIEKLQQKGHTVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISVGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIY"
     gene            complement(2103..2336)
                     /locus_tag="IWI22_002761"
     CDS             complement(2103..2336)
                     /locus_tag="IWI22_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003106687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAP9170079.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGVDKDSIKVLFNSSKV
                     KANFDSAVTSIEEIQKAIENVGYPVLKAKVKAA"
     gene            2575..3615
                     /locus_tag="IWI22_002762"
     CDS             2575..3615
                     /locus_tag="IWI22_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HAP9170080.1"
                     /translation="METERQIRELEKAMNTIDNRRLYERFLAVKLVLEGHTRKETGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKAGSKKQQVFLE
                     ETFPSLKKKLLNSDIDHLLFEDETMIRDYQAIGCTWFLRGHQRKIPTYGQHKGVKLIG
                     TLDYETGRIICTEEGAYDAAAFLRFLERTLREYPTGKIVMVLDNALIHHAKLIQSFLE
                     ENARRLALIFLPPYRPQLNLMEGVWKWLKESVINNVFFDHVQKIKQSVRGFLADVNER
                     PLEVIDRLCVRM"
     gene            complement(3930..4529)
                     /locus_tag="IWI22_002763"
     CDS             complement(3930..4529)
                     /locus_tag="IWI22_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9170081.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLSDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFLEQ
                     LQVQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTVVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
BASE COUNT         1263 a         1106 c          912 g         1433 t
ORIGIN      
        1 ttaatatatt tttactttct ctgcaggtgc tgatacttct ttttgttctt tccgaccttt
       61 caacttatac ttcattaatc tcatgccatt gaaaattacc accaagatac taccttcatg
      121 aaccaacatt ccgatagaca tattcatcca ttcgctgaag aagacgctgg tcaacaagac
      181 caacactact ccaactgaaa tcacgatatt ttgtttcata ttatttgcgg tggcttttac
      241 taatcctaat gcgtggggca agttgctgaa gttcgagttc attaaaacga catcggatgt
      301 ttcaatcgct acgtccgttc cgcttcccat tgcaatcccg atatctgcta gcgccaagga
      361 cgggctgtca ttgacaccgt ccccgacaaa ggcgaccgtg tgcccttttt gctggagttt
      421 ttcaatataa gccgctttgt cttccggcag catatgcccg tgcgcctcgg tgaggttcag
      481 ttcacgcgca acccggtcca ctgtgccttg gttgtcgccg gaaagaacca ctaaatcttt
      541 cacgcctaaa ttcttcaatt cctgcaagtc ttgtttcact cctggacgga tttggtcgcg
      601 gattcccatc agaatcttca attccccgtc gactgctgtc aatactaagg agttcccatt
      661 tttctcgaat tgtttgacgt ctttctgcgc ttttttactc aactgcacat tttctttttc
      721 catcaacgcc acatttccaa ccgcgatgcg gtgtccgtcc acttgagcga caacgccgcc
      781 gcctttgacg acgtctgtgt cttctaccgg gaacagttct accgtgccgg tatgctggag
      841 gacggccttg gctagtggat ggtctgattc agtctccacg ctggctaaat aactgtaggc
      901 tttttctaca ttgtctccgt acacttctgt ttccgcgact tcaggattcc cgacagtcag
      961 cgttcctgtt ttgtcgaaca caatcgtatc aactttgctg aagtcattga tgacttcgct
     1021 tcctttcaaa agaactccat tgcgtgcacc gtttccgatt ccggctacgt tggagaccgg
     1081 aaccccaatg accaatgcac ctggacagcc taggactaag atcgtaatcg ccaattcaac
     1141 gttttgggtc attgcccaga caatgaatgc cagaaccaaa acagttgggg tataccattt
     1201 agaaaagcgg tcaataaagc gctctgcttc tgatttagaa tcttgcgctt cttccacaag
     1261 ttcaatgatt ttaccgaatg tggtgtcttc tccgactttg tctgcccaaa tctgaatggt
     1321 gccattttcc aatatcgtac cggcaaagac ttcactgcct tctgtttttg tgacaggaac
     1381 ggattctcct gtgatactgg cttcgttgat atgcccttca cccgtaagga ctgtaccatc
     1441 tactggaact ttcgcaccag ttttgacaag cagaacatct ccttcatcta catcatctac
     1501 atccacttct tcaaattctc cgtcttctgt ttggaccaag gcactttctg gtgccatgtc
     1561 ggttaattct ttgattgctg aacgtgtctg gttcaatgta cgctgttcca aataatgtcc
     1621 gaacaagaac aagaacgtaa cgatagccga ttcttcataa ttttgaataa aaagcgcacc
     1681 aatcgctgca atactgacta agacatcaat actgatgact ttgactttca ttgcttgaaa
     1741 cgcttgaatg gcaattggcg taatcccaaa gacagaggcg atgatcaaag accactctga
     1801 aagtcctacg ttttctaaaa caaagtgact gaagaaaccg agtacgatta acaatccact
     1861 gatgacggtg ataacatttt tcttgcttaa tatatattgt tgcatgcttt ttccctccaa
     1921 atttaatttc tcttttattg ataaacacag tataagataa ttgcctaagt aattaattga
     1981 ctgacatcaa gtttagaaag ttctctttta tcgtttctaa gcacctcgaa tcggattgca
     2041 acaaaaaaag caatcctgga aaaatagctt ttcttccaga attgcctagt tcatttaatc
     2101 tgttaagcgg ccttgacttt tgctttaagg accggatagc ctacgttttc gatagctttt
     2161 tgaatctctt caatcgaagt gacagctgaa tcaaaattgg ctttgacttt gctggagttg
     2221 aataatactt taatgctgtc tttatcaaca ccgttaactg attttacggc accttcaatt
     2281 ttttgcatac aacttggaca agacaacgtt tctaattgca atatagcttt ttccataatt
     2341 cctatctcct ttatgtgttt ttattttatc ggcagcagat taatgaacac tgccttttcc
     2401 tttttacaac tgtagtatag ctcactttta gcaaacgaaa attgacacat attaagtttt
     2461 gcacttcttt ttatcttttt gctcaattca ccatcttata caaattgagt taaattgtat
     2521 tcgtttttcc tcctataggc gtatactaaa gaaaacactt gaggagtggg gaacatggaa
     2581 acagaacgcc aaatccgtga attggaaaaa gcgatgaata cgatagacaa ccgccggttg
     2641 tacgagcgtt tcctggcagt caagttggtt cttgaaggcc acacccggaa agaaaccggg
     2701 cacaccatcg gccgcgacga gcatactgtc agccactatg tgaaaaacta ctgccataat
     2761 ggaatccaag gtttagtctc caaaaaacaa agcggacgac cttcacggct gacagatgaa
     2821 caggaagcgg aactggtcca aatcgtcgcc taccacactc cggaggaagt aggtttcaaa
     2881 tcccgtgcca attggacatt ggcgattgtg tgtgacctga ttctccggga atggagttat
     2941 ggatatacgc aaaaaggcgt ggcggacctc cttcaccggc taggactaag ctggacccgt
     3001 ccgacgtata ccctcaaaaa agcgggttcc aagaaacaac aggtattttt ggaagaaacc
     3061 ttcccgagcc taaaaaaaaa attgctgaat agcgatattg accacctcct attcgaggac
     3121 gaaaccatga tccgggatta ccaagccatt ggctgcacct ggtttctccg cggccaccag
     3181 cgaaaaatcc ccacttatgg acaacacaaa ggggtaaaat tgataggcac cttggattat
     3241 gaaaccggtc ggatcatttg tacagaagaa ggggcatacg acgcagcggc cttcctccgt
     3301 tttctggagc ggacgttgcg ggaatacccc accggaaaaa tcgtgatggt attggataat
     3361 gcccttatcc accacgccaa gctgatccag tcttttcttg aagaaaacgc taggcgcctg
     3421 gcactcatct tcctccctcc ctacagacct cagttgaacc taatggaagg cgtttggaag
     3481 tggctcaagg aatcggtcat caacaatgtt ttttttgacc acgtccagaa aatcaaacag
     3541 tcggtccgcg gtttcttagc cgatgtaaac gagcgtcccc ttgaggtgat tgaccgactg
     3601 tgcgtgcgga tgtaaagtat ttaattcaac ctatatagaa agaagtcttg tacttcattt
     3661 tagtctaatg acagtcataa catcatcgac agccccgtta ttgagtcaat tctattcaat
     3721 aatttcttct acttgtaata acgttgtatt caacgcttaa aatattccag cattaaacac
     3781 cagttccaca ctgttctttt tcaatagata caccgattct ttgcgagata attggtattt
     3841 atcaggagat ttaggacggt tttctacgtt cattttccca cctcttgaat ttttccttct
     3901 attatatagg tatatagggc gttattctgt catagatcca aactctttac tgcggtatct
     3961 ttgtcttcct gggtgatccc aatatagcgt aaggtttccc gttgtgaaga atggtttagt
     4021 agtgactgaa tgatctctgt tttggttcct tgcttgtaca aaaaatagcc aaaggtctta
     4081 cgcatcgaat ggggaccaat ttctttcaac ccaaccaccg ttcctgcttc atgaatgatc
     4141 cgccaaaggc tttgtcgggt caaaggcgta tctgctcctt ttcgacttgg aaacaaatac
     4201 ttgctgaagt cttgtacttg cagctgctca agaaattctg attgaacata atcaagaatc
     4261 acttttcgta atttgttcga aatgaaaacc gaattgtgtt tagctgtttt ttgttccttc
     4321 atggccagac gttctttgac gatcacccgg ccacgggaaa tttctaacac gtcacttaat
     4381 ttcaactgtc ttaaatcgga gattcgatac gccgtattga tcccgatcga aaaaagcacc
     4441 aaatttcgtt gcccatattt tccggaagcc aaaattgcct tcatggcgtc gatctttttt
     4501 ttatcgcgaa tcggttccac gctcttcatt tttagtcccc tctcaaatgt tacttaatat
     4561 gattataaca gataataagt cacatttaaa caagctatag aaaaaaggcc ctaaaagaaa
     4621 agtgatttcc tcttagagcc tctaaggatg gagcgatttt gatgaatgtg acagaatccg
     4681 attgtgtcac attaggcttt tgatattttc ggta
//
LOCUS       DACDNO010000123         4584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDNO010000123 DACDNO010000000
VERSION     DACDNO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.125"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..702)
                     /locus_tag="IWI22_002764"
     CDS             complement(<1..702)
                     /locus_tag="IWI22_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAP9170082.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYD"
     gene            complement(699..1352)
                     /locus_tag="IWI22_002765"
     CDS             complement(699..1352)
                     /locus_tag="IWI22_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9170083.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1640..2914
                     /locus_tag="IWI22_002766"
     CDS             1640..2914
                     /locus_tag="IWI22_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9170084.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            2969..4534
                     /gene="guaA"
                     /locus_tag="IWI22_002767"
     CDS             2969..4534
                     /gene="guaA"
                     /locus_tag="IWI22_002767"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9170085.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         1443 a          872 c          768 g         1501 t
ORIGIN      
        1 gtcatatttt aacttttcta attttaaaat ttctttattt ttaaattcca aatcgtcaat
       61 tgttcttaat aaagtagaat acaaatcatt aatttgttgt tttaattctc caacttcatc
      121 attagagcta acttttaaac tcattttttt atcaagtttc atcattttat cagtaacaat
      181 ttttatttct tgtatattat tttttattga ttttgcataa attaaagaaa taatagcaga
      241 aaataaaatt gatattaata aagaatatgg taaaaattta aggcttaaat ctttcgcatc
      301 tttttgcata tcagctgtag aaacaaattg tagatttgtt tttttaccat cattaagctt
      361 tatttctctt tcttcaatta tcaaagaatt actatcactt tctaaattaa catttatatt
      421 atctttgatt tgtatttcat tcttattatt tttttctttt atgaatactt ttatttcact
      481 actcttggaa taaaactcca aagtttgttc tatatatttt atttcttttc cattcatatt
      541 actagaaatt tcatttgcca tattatgaat cttttctttt ctcgtctcca aatatgtttt
      601 aggaaagata aaaaaaatta atgaatgaac taatataatt attattccaa gaacagaaaa
      661 tatttgtatg aacatttttg gaaatatctt taattttttc attttctctc caatttatat
      721 cctacatttc ttatagtagt tatacagtct aattgtaatt tttttctaag ttcttttata
      781 tatacatcta ttactcgatc ataaggagtt tcttcgctat ctttccatac ataatcaata
      841 atttgcattc ttgttaacac ttgtccatta ttatccaata aacattttaa tacttctagt
      901 tcttttgcat ttacatctat ttcttcacca tttattttag ctgtatagct attaaaatta
      961 accgatagat ccttatattc aaacttctct aaatgtccat aattcttctt aattaaagaa
     1021 tctattctag cttttaagac cggcaatgaa aatggctttt ctacatatcc gtctactaaa
     1081 ttagtaaatg catcaatttt atattcttca tcactaaatg cagtcaagat tagaattggt
     1141 aaattgcttt tctttctaat ttctttcaac acttctaaac catttataaa aggtatctga
     1201 atgtctaaga taaccaaatt tatatcgcta ttaaattttg acaaggcttc tcttccatct
     1261 ttagcttgaa taacagtata tccaaattct gaaagatatt cacttattcc ctctcttatc
     1321 atattatcat cttcaacagt taatattttc atatatacct ccacttaagc ctatacacta
     1381 gatattatac agcatattat ggctctataa tatctagtgt tggtgacacc ttctgttgcc
     1441 aatacttttt cttttttcta ataaatgcaa acagacacct tgaaatcctt tagaataaag
     1501 ggttctataa tattttgtgt tgttggaaaa tcgaaggcga ttttctacta acactttttc
     1561 ttcgtttcaa ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca aaacatcacc
     1621 ctaccaaagg agtcgctcta tggataatca tactagaaaa ttactcggat taacagataa
     1681 acatttattt tttgatgaag aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca
     1741 gtttattaaa gggagattgg atgactcacc ttcccattgt gaacactgtg gatcgattcg
     1801 tattatccgc aacggttcct atacgacacg tactcagata ttgaaagtca aagaaaagtt
     1861 gactatttta gaattaaaac gtacccgttt tctatgctat gattgtgggc agaccttttc
     1921 tgcgaaaacg gacttagttg atgaacatca tcaactaaca aaggaactga agcaggcgat
     1981 tctcatagaa ctgtacgaaa atcaatcacg taaactaatc gctaagaagt acttcgtctc
     2041 ggatgggacg gtgacacgga tcttgcgtga agcaacgaag cattaccagc cacgtatgaa
     2101 cttccttccg acggtgttgt gtatggatga gttcaaatca atgaagtctg tttctggttc
     2161 catgagtttt atttgtgttg atgggacaac ccatcagcta tttacgatcc ttgaagatcg
     2221 cagactttac aaactaactc aatacttcct gcgctttcct agaaaagccc gtttaaaggt
     2281 caagtaccta gtgatggaca tgaatgccag ctattgtcag ttgcttaaaa cagtctttcc
     2341 aaatgcggag atcgtaactg accgctttca tattgtccaa cacatcaatc gcagcttcaa
     2401 tcaattacgt gtccagatca tgaatcgttt tcgaacctca cactctgaaa atcagaagaa
     2461 gtaccgtcgg ctcaagcgat attggaaact cttactcaaa gattccacga cattggagcc
     2521 actcaaacgg cattatcatc gactgtttaa acgaccaatt agtcaaacag aaatcgttga
     2581 tgagctgtta agctacaacg aagaattgcg aactgcgtat catttttgtc aattattacg
     2641 ctattatttc gttaaacgag gtacggaagg ctttcaagaa acccttaaaa caatttcatc
     2701 tacgttacct cagtcgttta aaaagaaatt cacgatcttc catcgctacc aatcgggtat
     2761 ttcaaatgcc tttaaggtgt cccactcaaa cggcgtcact gaggggctga ataacaagat
     2821 caagctgatt aaacgaattg cgtttggtta tcgcaacttt tataactttc gggcacggat
     2881 ctatttacaa caaggtctga tctttgaaaa ctaaaaaaat atgcggcgca cagaacgcgc
     2941 cgcatatcgg actaagtttt attcaaattt gtcttcacca acacgatttg acgaagagcc
     3001 aaaaattatc gtacttgatt acggtagtca gtataaccaa ctgatcactc gccgtattcg
     3061 tgagttcggt gttttctcag agttgatgag ccaccgcatt accgcagaag aagtaaaggc
     3121 aatgaaccct aaaggaatca tcctttctgg cggaccaaac agtgtttatg atgagaaggc
     3181 ctttggtatt gatgaaaaga tttttgattt ggggatccca gttttaggaa tctgttacgg
     3241 tatgcagttg attacttata aattaggcgg aaaagttaaa ccagccaaaa accgtgaata
     3301 cggaaaagca gaattggaaa tcacttctgc agaagcggcc cttttccaaa acactcctga
     3361 aaaacaaacc gtttggatga gtcatggtga cctagttacc caagcaccga ctgatttcga
     3421 tgtcgttgct accagcaaag attgccccat cgcatcgatc caaaatgcag aacgcaaaat
     3481 gtacggaatc caattccatg cagaagttcg ccattccgaa tatggaaatg acttgttgcg
     3541 ccattttaca ctagatgtct gccaatgtac tggtgactgg acaatggata acttcatcga
     3601 catggagatc gcaaaaattc gtgaacaagt cggtgacaaa aaagttttat taggcctttc
     3661 aggcggagta gattcctctg ttgttggtgt tcttttacaa aaagcgatcg gcgatcaatt
     3721 aacatgtatc ttcgttgatc acgtattgtt gcgtaaaggt gaagcagaac aagttatgga
     3781 aagtcttggc ggaaaatttg gcttgaacat tataaaagtc gatgccaaag aacgtttctt
     3841 atcaaaactt gctggcgtgt ctgatccaga agaaaaacgt aaaatcattg gaaatgaatt
     3901 tgtatatgtc ttcgatgatg aagcaaccaa acttgccggt gaagaaggcg tttctttcct
     3961 tgcacaagga accctttata ccgatgtgat cgaatcagga actgaaactg cccaaaccat
     4021 caaatcacat cacaatgtag gtggattacc tgaagacatg caattcaagc tgatcgagcc
     4081 attgaacaca ttgttcaaag acgaagtacg tgaattaggt acccaattag gcatgcctga
     4141 cagcatcgtt tggcgccaac cattccctgg accaggctta ggtattcgtg tccttggtga
     4201 attgacagaa gaaaaattag aaatcgtccg tgaatcagac gctatcttgc gcgaagaaat
     4261 cgccaatgcc ggtttagatc gcgacatctg gcaatacttc accgtcctac caggcattcg
     4321 ctctgttggg gttatgggag atggccgtac gtatgactac actgtaggta tccgtgctgt
     4381 cacttctatt gacggcatga ccgctgactt tgcccgtatc ccttgggaag tcttgcaaaa
     4441 aatcagtgtc cgtatcgtaa atgaagtcgc acacgttaac cgtatcgtgt atgatattac
     4501 cagcaaacca cctgcaacag tggagtggga ataagacgta aatataatat ggatggggaa
     4561 tgccgataaa tcaacgtttt tcaa
//
LOCUS       DACDNO010000124         4577 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDNO010000124 DACDNO010000000
VERSION     DACDNO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.126"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            234..1115
                     /locus_tag="IWI22_002768"
     CDS             234..1115
                     /locus_tag="IWI22_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9170086.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1128..2738
                     /locus_tag="IWI22_002769"
     CDS             1128..2738
                     /locus_tag="IWI22_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9170087.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2815..3474)
                     /locus_tag="IWI22_002770"
     CDS             complement(2815..3474)
                     /locus_tag="IWI22_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9170088.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3757..4422)
                     /locus_tag="IWI22_002771"
     CDS             complement(3757..4422)
                     /locus_tag="IWI22_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170089.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1252 a          874 c          888 g         1563 t
ORIGIN      
        1 ttaaactttt ataatatata tagttcaaat ttattctgag gtttattata actatctatt
       61 gagaaagtat caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa
      121 gaatattatg tactaaaata ataaaaaaag ttttataaaa aggtataaaa cctttttatt
      181 aaactattta atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag
      241 aaatcgtcaa agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg
      301 tctctttcac cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa
      361 aatccacgac gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga
      421 tttttgggca agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc
      481 caggcgatat ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga
      541 agcttcatgg aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg
      601 atcctaagaa aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg
      661 ctgctttggc tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc
      721 ctttgatgga aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca
      781 tcctactttc ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca
      841 tacgcgaagg aacactcgat gaagaaggaa cactcgatga attgcgtcat ttgactcgtt
      901 cgaccatcac tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc
      961 atgactttgt gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg
     1021 tgattttagc tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc
     1081 ttgaggattt attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat
     1141 tttcacgctg gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga
     1201 tatggctagt cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga
     1261 cttccggaca aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa
     1321 tggtcggacc aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa
     1381 atgaaatgtt gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga
     1441 gccaaacgag aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg
     1501 gacgtcaagc aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag
     1561 caatatcgat cagctgtacg atgatttttt tccaagcgga ttcgatcact gcagaaggtg
     1621 cttggctttt tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt
     1681 ttgctgcaca actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag
     1741 gtggacttta ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga
     1801 atcctttagg ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc
     1861 tttatttgtt tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc
     1921 gagatttgaa caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc
     1981 ttttatcctt gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta
     2041 tcacttttgg tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt
     2101 tagaaagtaa cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa
     2161 actttacaag cacgatcatg gtcgtcttga tttgtctagc tgctatttta ccaattgcta
     2221 gcatcaatcg tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa
     2281 aaatttctag gaaccaactt tattggacca atatgttact agctattcta tttgctatat
     2341 tggcaatcct gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg
     2401 cgaaagacat ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt
     2461 tagtgtttat cggaattagt ggctttctat tcggatggct tcctcgaatc ggtaaacttg
     2521 cttatatcta cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac
     2581 cagaatggtt ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt
     2641 ttgatctttc cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt
     2701 ctattggtta taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca
     2761 tgctagcctc caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa
     2821 gtcttctaac tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc
     2881 attctcctct aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc
     2941 agcaagttct ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa
     3001 gtcaagcaga tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg
     3061 ctgctgtact tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg
     3121 ataatgcagt aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc
     3181 tagtgcttcg ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata
     3241 tccgcccggc tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac
     3301 tttcatactg ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg
     3361 ttctcctttt tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg
     3421 tactaagtga ttgaacaaag gaacaaatga aacacataaa tgatcatgac tcataatctc
     3481 tcctccttct aaacagctat ttctaataaa gaaacataac ttcccttatt ccaactgata
     3541 attttctttt atcatagcat aaacgtcaag cgaaccagat accagcaaat agagtggatt
     3601 tctttttgtc ccttgcttta ttttccttct tttgagacct taagtaattg attcctatca
     3661 attttttaaa tatttacttt ttgaataatc aaaaacaaac cttcactcgc aagccgagag
     3721 taaggtttgt ttttcgcatt gcttatttcg tgttgtctat tgaaattttt ccgcacctag
     3781 cttttgatat agtggtgcta gttcttgtaa ggtttcagcg gttatttgct caacgtgttc
     3841 gatttcttgc ggatagaaga atagtttacc catttgaaag gttcggtctc tcatcgcttt
     3901 tttcaactct tcttgtttct ttgtatcgga caaatagtca gacaaagcta agtggtcgtc
     3961 aagttcatgt tctggttgcg gccatatgta ataatccttg atatcgactg ttttgctcca
     4021 ctctttcagt atcgcaagca actggttata cgctgcaatg ctaccatatc gttcttcgct
     4081 tttataatgt tggtacatca aatagatttg gtattgtttt tgattcaaca aaactgctag
     4141 acaggcattc tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa
     4201 attccagcca ttcgtccagc tttcgatttt aggtttgctc atgccgtaca tatcaggcaa
     4261 aagtgcagct gtctggagct gaatctcttt ccatttttgc cagtgttctt tatattcttt
     4321 cttgatttgt tcaatcttat ctgttggatg gctctctttg agctgtttga atgcgtagat
     4381 tggtcgatca aacaactgat aatctgattt tccaagttcc attgtttcgc ctctttccgt
     4441 tatcttttgt attctacctg aattttgcga tatggacaaa tagaggttgt gaaaaagctg
     4501 ttctgcatca agtcataaga acagccaaac aaaaaactgc gatgagcatc accgcagttt
     4561 tattggctct ataattt
//
LOCUS       DACDNO010000125         4492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDNO010000125 DACDNO010000000
VERSION     DACDNO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.127"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..457)
                     /locus_tag="IWI22_002772"
     CDS             complement(62..457)
                     /locus_tag="IWI22_002772"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP9170090.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            complement(467..1315)
                     /locus_tag="IWI22_002773"
     CDS             complement(467..1315)
                     /locus_tag="IWI22_002773"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP9170091.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            complement(1330..2157)
                     /gene="panB"
                     /locus_tag="IWI22_002774"
     CDS             complement(1330..2157)
                     /gene="panB"
                     /locus_tag="IWI22_002774"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP9170092.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            complement(2169..3029)
                     /locus_tag="IWI22_002775"
     CDS             complement(2169..3029)
                     /locus_tag="IWI22_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HAP9170093.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQEIKNYQIKNKLICHCSGAISS
                     DIFSNIQDYGAYGYSVHPMFSISDKYNSYKKLKKSFITIEGDEKYAKFLCDFFKKLGN
                     STVIVSKENKSLYHAASVVSSNLVLGLINTSVTYLIQCGFTEKMAIEALYPLIEFNIR
                     NIKEKGIIESLTGPVERCDISTIKGHCEVLSDEDRALYMLLSKNVLEIAMLKNINRDY
                     SELEKYLGES"
     gene            3267..4436
                     /locus_tag="IWI22_002776"
     CDS             3267..4436
                     /locus_tag="IWI22_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9170094.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRDRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT         1466 a          844 c          559 g         1623 t
ORIGIN      
        1 aattagtaat caataaatat ttcaataata gtctagctat atataaataa cattacaaaa
       61 cctaaagaat tatatctgat aaccttccat gtttttcata tgatgttact ctttcaactt
      121 tattattatc atcgacaaaa acaaccttag gtctatgatc cttaacctca ttttcattta
      181 attcacaata tgccattaaa attattttat ctccagttga tacacatcta gctgcagctc
      241 cattcaagca aatcatccct gttcctctct ctccagctat tgtataagtt tcaaatctat
      301 ttccattatt aacatcaact atttgtactt tttcatattc atatattcct gctgcttcta
      361 ataaattcat atcaacagta atacttccta cataatctaa ctcagcttgt tcaactgtag
      421 ctctatgtat ttttcctttt aacattgtaa ctttcattat agccccctat tgtaattcaa
      481 atgtaaaatt atctattaat cttgtttttc ctataaatac tgctattgca actaaaactg
      541 atttttcaat ataatttact ctttgtaatg ataaagaatc aacaacttct acataatcaa
      601 tctttgccaa attataagta tttatatttt tacttattat ctcaattatt tttaagctgt
      661 ctctctctcc attatttaaa gcttccttag ctaaagttaa agatttattt aaaatagtag
      721 ctgaagatct ttcctctaaa cttaagtatg tgtttctaga acttttagct agtccatcct
      781 cttcccttat aattggacac cctacaacgt ctatatcaat attaaggtcc ctaaccattc
      841 ttttaattac agctaactgt tgtgcatcct tttctccaaa ataagctcta tctgctggta
      901 ttatgttaaa taatttactt acaacagtgc aaacccctct aaaatgtatt ggcctactag
      961 ctccacataa accttctgtt aatccagtta tattaacaaa agtacttgca ttgtcacaat
     1021 acatctcttc aacttcagga ttaaatattg ctgttgctct acctcttgtg catacttcta
     1081 tatctctttc taagtctctt ggataactac ctaaatcttc atttttccca aactgagttg
     1141 gatttacaaa gatacttaca acaactttat cattttcttt tgatgctttt ctaattaaac
     1201 tttcatgacc ttcatgtaaa tatcccatag taggaactaa tcctaccttt aagccttgtt
     1261 ttttccactc ttttacttga cttctaacat catttactgt tttaaatata accatatttc
     1321 ttgccccttt taatataatt tttctaaaac atcatcactt attttaaacc catgttctgg
     1381 acctggaaaa actccatctt ttacttcctc tatatatttt gtaaatgctt tattcatctc
     1441 ttctgataaa ttagcatatt tcttcacaaa tttaggggta aaatcactat acatccccaa
     1501 catatcttga tatacaagaa tttgtccatc acaccctgca cctgctccaa taccaattgt
     1561 aggtattgat atctctttag aaattaactc tgcaagctta gttggaacac attctaacac
     1621 tacagcgaat gctcctgctt tttctacagc tttagcagct cttattaatt ctttagcagc
     1681 ttccttgtcc tttccttgta ccttaaatcc tccaaatgca ttaacagatt gaggtgttaa
     1741 acctatatgc gccataacag gtatagaagc ctttactata gcctctattt tatcgcatac
     1801 ttctatccct ccttctaatt taacaacctg agctcgtcct tcttttatta atcttcctgc
     1861 attaactact gaatcatata ctgaagtttg ataagacata aatggcatat ctgcaacaac
     1921 taaagtgttc ttagctcctc ttgtaactgc tctagtatga tgaatcatgt cttccattgt
     1981 tactgaaagt gtatcttcat gtcctagaca taccatacca agagaatctc ctactaatat
     2041 tccatttatc ccagcttcat caattatttt tgctgttgaa tagtcataag ctgtaagcat
     2101 tgttaatttt tcatttctta atttagattc tttaaatgta actgctgtat ttttcatcta
     2161 tttctctcct aactctctcc taaatacttt tctaattcac tataatctct attaatattt
     2221 tttaacatag ctatctctaa tacatttttt gataatagca tatataaagc tctgtcttca
     2281 tctgataaaa cttcacagtg acctttaatt gttgaaatat cacaacgttc aactggccct
     2341 gtcaaacttt ctataattcc tttttctttt atatttctaa tattaaattc aattaatgga
     2401 tataaagctt caattgccat tttttcagta aatccacatt gaattagata tgtaacgctt
     2461 gtattaataa gtcctagcac aaggttacta gatactactg atgcggcatg ataaagagat
     2521 ttattctcct tagatacaat tacagtacta ttacctaact ttttgaagaa atcacataaa
     2581 aactttgcat atttttcatc gccttcaatt gttataaaag atttttttaa ttttttgtat
     2641 gaattatatt tatctgatat tgaaaacata ggatggacag aatagccata agcaccatag
     2701 tcctgtatat ttgaaaagat atctgaagat attgcaccac tgcaatggca gattaatttg
     2761 tttttgattt gataattttt aatttcttgc catacctcct gaatttgacc atcaggagtc
     2821 gtaatgaaaa tagtatcatt ttcatctact aattctctta agtttaaata ttgtttacta
     2881 gatgtaaaat tcgaagctgc taatgacgag tcttcgcttt tactataaaa tcctgttact
     2941 tgtattttat gttcctggaa atacttcccg aatgaacaac ctaccttgcc ggctccaata
     3001 aaaccaacgt tcataattat cacctccata aagaagtgag actacaagtc ccattcttac
     3061 gatatgagct taaaaataaa aattaaatca aatatacagt atggtttttt caaatatcat
     3121 ccatagttaa tttatcataa tatcccatat tttaaaataa aagatagcgt aaaatatttt
     3181 gtgtaaatag aaatttaggg tagaaaaaaa agtccattct gtaaaattaa agttaccaca
     3241 cagaaatttt ataggaggac tttctcatga ctaaatttac tatagaaatt atgcaaacac
     3301 tactcaataa aggggattta gatgaattat ttcaggatca tttagagcga gcgattaatt
     3361 cacttctaca agcagagcta actgcttttt tagattacga aaaatatgat cgaaatggat
     3421 tcaattcagg aaattcccgt aacgggaatt attcacgtac atttaaaaca gaatatggcg
     3481 agttgaattt aactattcct agagatcgga atggcgaatt ttctcaacaa acactaccag
     3541 catataagcg aaccaatgat tcattagaaa cgaccattat ccaattattt caaaaaggca
     3601 ttactatggc tgaaatctcc aaattaattg aaaaaatgta tggtcatcac tatacgccac
     3661 aaacaatctc caacatgagt aaattggtgg ctgaagatgt tttagctttt aaagaaagaa
     3721 cgttagaagc caattattcc gttatattta tggatgcgac gcatatccct gtgaagcgac
     3781 agactgtttc gaaggaagca gtgtatatta cgattggtat tcgtttggat ggaaccaaag
     3841 aagttcttgg gtttactatt gccccaactg agtctgctta tatttggaaa gaagttcttc
     3901 aagatcttag aaaacgtggg ttagaagaag ttttattagt agtgacagac ggattaagct
     3961 gtattgaaga aagtatccat agtgtgtatc cgaatgccca atttcaacaa tgttgtgtgc
     4021 atgtatctag aaatatcgct cataaagttc gtgttcgaga tcgaaaagaa atttgtgagg
     4081 atttcaaatt ggtttaccaa gcgaattcaa aagaagaggc attggatcac atcgacttta
     4141 tgactaggaa atggaaaaag cagtatccaa gagtcgtcaa tttactcttg aatcctgccc
     4201 tattaacctt ttataatttc cctcacgcca tcagacgaac aatttattcg acgaacctga
     4261 ttgaaggctt taataagcag ctaaaacgat atactcgaag aaaagaacaa ttccctaatg
     4321 aagaatctct agagagattc ttcatttctc aatttaatca atataaccaa aaatttttag
     4381 gtagaattca caagggattt aaagaaattc aggatacatt agagtcgatg atttaactgt
     4441 aattaacaga atggattttc catttacaca taattcttga cgctaccatt tt
//
LOCUS       DACDNO010000126         4389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDNO010000126 DACDNO010000000
VERSION     DACDNO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.128"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            85..972
                     /locus_tag="IWI22_002777"
     CDS             85..972
                     /locus_tag="IWI22_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9170095.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IWI22_002778"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IWI22_002778"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9170096.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IWI22_002779"
     CDS             2274..2720
                     /locus_tag="IWI22_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9170097.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IWI22_002780"
     CDS             2993..4345
                     /locus_tag="IWI22_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9170098.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         1499 a          727 c          931 g         1232 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtcttt
//
LOCUS       DACDNO010000127         4334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDNO010000127 DACDNO010000000
VERSION     DACDNO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.129"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            26..1365
                     /locus_tag="IWI22_002781"
     CDS             join(26..467,467..1365)
                     /locus_tag="IWI22_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9170099.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            complement(1723..2331)
                     /locus_tag="IWI22_002782"
     CDS             complement(1723..2331)
                     /locus_tag="IWI22_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP9170100.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(2337..3365)
                     /gene="vanB"
                     /locus_tag="IWI22_002783"
     CDS             complement(2337..3365)
                     /gene="vanB"
                     /locus_tag="IWI22_002783"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP9170101.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            complement(3358..4329)
                     /locus_tag="IWI22_002784"
     CDS             complement(3358..4329)
                     /locus_tag="IWI22_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP9170102.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
BASE COUNT         1305 a          992 c          867 g         1170 t
ORIGIN      
        1 aatctaatta aaaggggtgc tttttttgcg gaaatataca tttgttttta agaaaaaagt
       61 agtttcagac tatttaaata acgaaggcgg ctacaaatat cttgcacata aatatcaaat
      121 aaatcgtaca ctggttagac attgggtaag gatttataac tatcatggtt gggaaggctt
      181 ggttggaggt ggcaaaagct acactacaaa atttaaactt gatgttatag aatatatgga
      241 aacaaatggt ctttctatcc aagaaactgc taaaaaattt aatatcggtt caaatagaac
      301 tctaagtaaa tggatagagc aatatgaaga aggcggtgct tcttcacttg agagccaaaa
      361 aaggggcaga aaaattagta tgaattccaa gctaaacatt cctaaaaaac ttaaagatga
      421 gtctcttgaa gaagaagtta ttcgtttaag agcagagaac gcatatttaa aaaagttaga
      481 aaccttgatt caagaacaag atttgagcaa gaagaaatca aggttaaaac aatccttcag
      541 ctcaaaaaag aatttaaatt gaatctatta ctttcaattg cacaattagc gaagtcaact
      601 tattattact gggtaaaaaa attagataag ccagataaat atagcaagat aaagcaagaa
      661 attacagcga ttgtaaaaga atcaagaaac tcttatggtt atcgtagagt cactttagcg
      721 ttaaagatga aaggatatac aatcaatcat aaaacagtta gaaaattaat gtcccaaatg
      781 ggacttacct gtcaaatccg aataaagcga tataaatctt ataaaggaac agtaggaaaa
      841 attgccaaga atgtgttaaa acgaaatttt tcagtagata cacccaataa aaaatgggtg
      901 acagacgtca ctgaattcaa gataaaagga agaaaaatct atctatctcc tatacttgat
      961 ttatttaacg gagaaataat tagttatagt atatcaacta gtccaacata taaactgatt
     1021 gaagaaatgc ttcaacaagc aattaaaaaa aagggaactg agggttcact gattctacat
     1081 tcagatcaag gatggcagta tcagatgcca caatatcaaa aaaaattaaa agagaataac
     1141 attattcaaa gcatgtctag aaaaggaaat tgcttagata attctgtaat agaaaatttt
     1201 tttggtgtgc ttaaatcaga gtttttttat cgagaaaaat tccgatcaat tgagatattt
     1261 caaagtaaat taaatgagta tattaggtgg tataataaca aaaggataaa actaaagtta
     1321 aatggactat ctccagtaga atatcggaaa cagtccatta aatagttcaa cttttggggt
     1381 tcagttcatg gaggggcaag gcttttttga gtggtggaag tggattagag catggcaata
     1441 gcaaaacagc atagcggcat cctcctatgg gccgccacac cttttgctgc cagaaaagaa
     1501 cggcggcctt gatttctcaa aaccgccgcc agcaaaatac aaaatgggcg tgggaaaggt
     1561 ctgcccaaca gcggtttttt tcttttctgc cgctgggcag attgaatact ataatttgca
     1621 atgtattaca aaagatgctt taaccacttt ccagtaaaag attgtccatg accagcatag
     1681 attttatcaa atccaaaatc tgatacaaaa ccaatacttt cattatgaaa cggcaaaatt
     1741 aaaataggta tcggggtatg gctcatcaat caacttatag tgccaccatt caaagcgata
     1801 agactgaaat ccgctgcttt ccatgatttt acgcaagcat cttcgatttt gtgcctctgc
     1861 atcccctatc ccttttgctg tatgatgcga ccgttcgtcc ataaaatcga agttacttcc
     1921 cattgaaaca agttcccctg tatccaggtg gtagagcgta agatcaattg cgcttccacg
     1981 gctatggctg gattgtgagg ccacataacc ctttgtaatc aactcggctc gctctatatt
     2041 ggggtaaaat ttttcttttg tgagattgtc ctccggttgc gctgcccaac gcaggaagca
     2101 gtccaccgca gttcttggcc gatatccatc ccataaaagc aagccatagc caagcgcagc
     2161 cgcatggatt tgtgcctcgc gcagagcaaa cgccacggcc tttgtgccga tgatgcgatt
     2221 cacctcatac ccatccaccg gttttcctgt gaagttatcc catgtggcgt acttggcatc
     2281 ccaacggacg ccatgcaaca tttcatctaa aaacaaaaaa ccattttcca tacttatcac
     2341 ctctttaacg ccaatgtaat caggctgtca atcagtgcag gaagcgtgat tcctgcggcg
     2401 gccaccatac gtgggtagcg gctgtacgat gtaaaaccgg gcatggtatt gacctcattt
     2461 agaacgatgc cgccatcctc ctgcaaaaaa agatcaacac gggcaagccc tctgcatcca
     2521 agcacccgat atactttctt tgccgtttcc tgcacccgat ttcgttcctc gaccggaatg
     2581 tctgcgtgaa ctgtaatcat cgcattttct gagccttttt ccggctcgtt ttcctgatgg
     2641 atgcggaaga taccgtggct cagccggatt tgatccactt cgccgacaat caaatcatcc
     2701 tcgttcccca tgaccgcaca cccgacctca cagcccgaaa tcgcttgctc aattaagatt
     2761 tttccatcat attgtcctgc cgcttctatc gcagcgttaa gttcttccgt accgtttact
     2821 ttggttacgc caaaggacga acctgaccgt gccggcttca caaagacagg gtaggtaagc
     2881 gcacccgcct ccggcttgtc acctttatca ataatttgaa attcgggaac ggcgatgccc
     2941 gcattttttg taagaatgta ggccagtgat ttgtccatgc aagctgcgga gctttgaata
     3001 tcacagccca cataggggat accagacaat acaaacagcc cctgtatcgc accatcctcc
     3061 ccgcatttgc catgcaaaac cgggaaagcc acatcaatac gccgtgtttc gtattcgctt
     3121 tctttcatga caagcagccc atgcgttttc ctatccgggg agagtatggc ggggagactg
     3181 tcggcttccc attccgtaca tggcttcttg catagcttcc atacaccgtt ttttgtaatt
     3241 ccgatgtagt gcggatcgaa tttttccgta tcaatgttcg cagcaatttc tattgcggat
     3301 tttaccgaca catcatgttc ctccgagcaa ccgccgaaga tgattgcgac ttttattcta
     3361 ttcatgctgt aaactccttt caaaattgag acaattcctg attgtttttt ctgtagtatc
     3421 ctgcagcaca cgctcagtgt agtacgccgt atggggtgtg atgatcacat tcggcatcct
     3481 taaaagctgc gacaaaaagg gatggtcaag cactttctgc gagcaatcgg tataaacaaa
     3541 ctgatcctcg ccctccaaca catccagtgc cgcaccaccc agctttccgc tttccaacgc
     3601 ctccaccagc gccccggtat cgacaagcgc tccgcgcgcg gtgttgatta aaaatgcgcc
     3661 ttgcttcatc tctccaattt gcttctgacc gatcagatgg cgggtatccg cacaaagcgg
     3721 cacatggagc gtaacaatat cgctgttttt tagaagctca tcaagctgga cataatctgc
     3781 atccattttt cggctgttat cataggccag cacatggcat ccaaatcccc gcagcctttt
     3841 gacgaccgct tgcccgatat ggccggttcc aataactccc acagtcatat cccccagttc
     3901 tttcccccgg atacgatcca atctgaaatc ttgttgcgcc acggcgtgca tggtgggttt
     3961 tgcaccccgt atggccatca gcatcagcat caaagcataa tccgcaacgc tgtccggcga
     4021 atacgcaacc gtgccaaccg agattcccat tctctcggcg gcagtcgtat caatgtgatc
     4081 gcagccgatg ctgcgggtag aaatgtattt taccccgacc tttctcagcg caagaattgt
     4141 cgcctcggaa acctcggact tatggcctac gctaacgcat tgattgccag cggccaattt
     4201 tgcgttgtct gccgatatcg catcgctaat cagcgtaggg ataatatgaa aatccggcga
     4261 taaagcgcgg aaagcatttg cctcatcctg ttcgcatcca aaaacagtaa tgcccacact
     4321 ttttctcatt ggtt
//
LOCUS       DACDNO010000128         4312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDNO010000128 DACDNO010000000
VERSION     DACDNO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.130"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            292..810
                     /locus_tag="IWI22_002785"
     CDS             292..810
                     /locus_tag="IWI22_002785"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9170103.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            938..1621
                     /locus_tag="IWI22_002786"
     CDS             938..1621
                     /locus_tag="IWI22_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9170104.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1930..2003
                     /locus_tag="IWI22_002787"
     tRNA            1930..2003
                     /locus_tag="IWI22_002787"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1964..1966,aa:Arg,seq:tct)
     gene            2162..2521
                     /locus_tag="IWI22_002788"
     CDS             2162..2521
                     /locus_tag="IWI22_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9170105.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2644..4275
                     /locus_tag="IWI22_002789"
     CDS             2644..4275
                     /locus_tag="IWI22_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9170106.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         1390 a          683 c          886 g         1353 t
ORIGIN      
        1 gataatataa tgattatgct ccactttata cgtaaataaa aagattcttt tttattatat
       61 atgttattaa aaaaagacat aaaggtattc ctttctaaaa cttgtaatgt tagggtaaac
      121 gttttttaaa ctaacgtcaa ttatttataa taaaaaatga gataaagatg aggaaataga
      181 attagaaaca tataattatt aaagggaaac atttttacag ctataatttg aaaaaaattt
      241 atcgttatta tatgattaag aaggattgaa taaagaaaga agttgaaaac gatacctgat
      301 tacaaaaaaa taacttttat aagttctata acgttgatta tatttttaat tcttattctt
      361 ctcgtcgtct tggttccccg aatttttggt ttgactcctt atattattga ggacaaagaa
      421 atgagtccat attatgcaca aggaaatctg atatatgtac gaccgaaaaa tacaaatgag
      481 attttagtcg gagatattat tacatattat gaaaattcag gaaaaaaaat ctcgacgcgt
      541 cgcgtaatag ccgttgaaaa caaccacgaa gatttttata caaaagctga tacaaagact
      601 tatattgagc ctggagtagt aaaaaagaga aatattatcg gtagtcccat ctttcagatt
      661 ccatatattg gactagtcct ttcaaaaacg gcatttgcat ggattcaggg gttattcttt
      721 acgattgctt tttgcctaac aggaatcaca gcatggaata cctttgtaga gttgaaaaag
      781 aagcggagac gaacacagaa atttttgtaa gaatgttaaa aacataaatg tattccagag
      841 atccaacgac tcttctgaac atgaaacacg agaaaacact catgcagctt gtagatttag
      901 tcagttgttc gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg
      961 tcctgtagtc aaagaagaac tattggattt tatgcgtacc cagcaaaaaa aattaccggg
     1021 tgagctaggc aaactggaag aagaagctca tgtagcagaa gtaccagtta ttccccatga
     1081 aacagttgta tttttgaaat ttttacttgg acagcttcag cctgaacgaa tcttggagat
     1141 cggagcagct atcggttttt catcaagtgt catggccacg acaatcggag aaaacgctca
     1201 tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg
     1261 tctaggactg acagataaag tgactttgct tgaaggtcaa gcagcagata tcttgccgga
     1321 gttgtctggt ccgtatgact ttatttttat ggatagtgca aaatcaaaat atattgaatt
     1381 tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag atgacatctt
     1441 ccaaggagga acgatcttgc tcccagatga agagattccc cgtggcaaac gagctatcca
     1501 tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat cccgatttga catcgacaat
     1561 cttgccatta ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg
     1621 atacatgatt atggcagcct gtagataatc gattgaatcg agggctcaat tcgaacttga
     1681 tgtaaaagtt tctatatttg taagtcaatt aaaggataca ggaaatctaa ataaacagga
     1741 gtagcatgaa gtcaatgtag ctcctgtttt caattgataa ccttagaaca gctgaaggag
     1801 aatgtctcgt tttaaaagat tgatttagta gttgggccaa aataattcat tatttgctaa
     1861 ttaaataact atttcgtatt gacagaagca atttaattat ctataattat tgatgttccg
     1921 ctgattcttg tcatagtagc tcagctggat agagcatccg ccttctaagc ggacggtcgg
     1981 gggttcgaat ccctcctgtg acgctaaaaa accgttagaa actgaggttt ctaacggttt
     2041 ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc aaataaaata aaaaaacatc
     2101 cttatcgagt acaattataa ggagcaaaaa aataaaactc atcagaaaag aggaagaaga
     2161 tatgacgata ctatctagaa ttttagtgac tctagttgca attgaatttt tttatatcat
     2221 gtatatcgaa acggtgagaa ctgattctga cacgacaagt agggttttta aaatgagtaa
     2281 agaagagtta agcagaaaat ctgttcaaac attgtttaag aaccaaggag tgtacaatgg
     2341 actattagga gtgggactac tttatggagc ttatctttct tccgcatcta aagaaatcac
     2401 aagtatgcta ttaatatcta ttttttttgt tgcattatat ggcagtttga ctagcgatcg
     2461 ttggatttta ctcaaacaag gtgggctagc tctgttggca ttgctttcta ttttcttata
     2521 aatatagaaa aaataagaat ttatctaaca ggaaactttg tgaaaaatat tactttatct
     2581 catgaaatat gatattctaa tgaatggtat tttagaaaat ttcatgaaaa aggagttaga
     2641 taggtgttca aaaattatgt ttcctcacta aaaaaagaat tttcaggtta tgatctttca
     2701 agatttcaga aagatctttt ggctgggatc acagtagctg ctgtagcatt gcctttagcc
     2761 ttggcttttg gggtatcaag cggagctgat gcggccgcag gtttgattac ggctatcatt
     2821 gccggtatcg ttattggcgg tttatcaggc ggtttttatc aaatatcagg tccgacaggt
     2881 gcgatggcag ctatcttgat gtctattgcg gcaacaaagg gaatgcaagg tgtattgctt
     2941 gctacttttt tagcgggtct atttttactt gcagcaggac ttttccgatt aggaacactg
     3001 acttctttta tcccagcacc cgtaattacg gggtttactt caggtattgc tatcattatt
     3061 gcattaggtc agatcgataa tttatttggt gttcactcgg aaggtacaaa cgttattgaa
     3121 aaactaagta gttatcaaac gataggattt ccgataaaca cagcctcttt gatcatggga
     3181 gggctagtca ttttagggat gtttatctat ccaaaaaaat gggcgaaacg aatgcctagt
     3241 tcgctgctgg ctatcatttt ggcaacagca gttatgctat tgttccattt accagtagca
     3301 actgtgggta agattccaca aacattgatc agtagtaatc gtttgaatat gggagatttt
     3361 agcttttctg ccttgcaaca agtggcagtt ccagctatta gcattgcttt attaggaatg
     3421 attgaaagct tgctatgtgg tgcttcggct ggccgaatgg caaataagcc cttagatagc
     3481 aatcaagaat taatggcgca aggaattgga aatctgttgc ttcctttctt tggcgggatt
     3541 cctgctactg cggccatcgc ccgtacaagc gtggccatca aatctggtgc acaaactaga
     3601 ttagctggta tgatacatgc agttgtctta ctgctctcca tgcttgtttt tgcgccagtg
     3661 atgtcacaga ttccaatgcc agcacttgca ggtgttttga ttgtgacggc ttggcgtatg
     3721 aatgaatggg aaacgatcaa ggagctattt actaataaat attggtcagc agttctattg
     3781 tttatcctta cgatgatttg tacagttatc tttgatttaa gtattgctat cgtgatcggg
     3841 gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg ctatcacgat ttcattagaa
     3901 acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa agctgaataa ctggacagta
     3961 gtgtatatta gtggtccact gttttttatg tcttcagaaa aactcaaaaa taaattggaa
     4021 gaactaaatc atcacgatgg tgtcattttc tccatgcgag gcgtaccaag tattgatttg
     4081 acggcacgtg cagtttttga tgaatttcaa gaaaaagcag agcaaagagg acaaacgatt
     4141 atctatactt ccttgcagcc agaagtagaa aaacaacttt ctcacttgtg ggaacataca
     4201 ttcacgcatc aacatcaaac agttgctcat gcgttgacag ctttacatgc gaaatatact
     4261 gcaattagcc attaaaacaa tgaaaaagaa cagacaagac tgtaaactga ag
//
LOCUS       DACDNO010000129         4211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDNO010000129 DACDNO010000000
VERSION     DACDNO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.131"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            27..473
                     /locus_tag="IWI22_002790"
     CDS             27..473
                     /locus_tag="IWI22_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170107.1"
                     /translation="MQQNRIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKM
                     SLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVS
                     YKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            487..1176
                     /locus_tag="IWI22_002791"
     CDS             487..1176
                     /locus_tag="IWI22_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170108.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1301..3652
                     /locus_tag="IWI22_002792"
     CDS             1301..3652
                     /locus_tag="IWI22_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9170109.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3732..4127
                     /locus_tag="IWI22_002793"
     CDS             3732..4127
                     /locus_tag="IWI22_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170110.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1480 a          532 c          851 g         1348 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg tattttttca gtatttacaa
       61 tattaattgg gggctatttt cttttgaatt tagattctgt taattcaaac gctcaaataa
      121 aagatcaaag agtagctgaa cataataata aaaaaatgag tttgaccaaa aatatctatt
      181 ctatgtcaga tgaagaattg tataaagaat ttggtgattt atccggggaa cagttagaag
      241 aaagtattcc aggaattagt gactcagata aaattgttgt cttgtctgct ggtgggcaat
      301 taatgggaga tggcaaatcc gaattagtta gttataaaca aaatcacaga cagtttattg
      361 atgttaatac tgattccaac gctatttcga ttaaggatct aaaagagctt ttaaatagta
      421 aaagaaatga attaaagcag ataaaagaaa ctaattcagt ggaattagag taaaggaaga
      481 ttcattatga aaaagatttt tggtgtttct gttgggctac ttatgatgtt aacatttgga
      541 aatgcagtat atgctgctga gaaagaagtt atttcagatc cagagcagta cgcctcacga
      601 tactttgctg attttgaaga agaagataaa gtacttgcat tgccagaagg tggtttttta
      661 aaaggggaag ctaaaattgt atctatggat aatccagata taattcaagg ttcatataat
      721 tcagaaactg atcctgcttc agtttcagtg acagaagcaa aaaaaattgt tgctgaaaaa
      781 ttcaaaaatg atattgaaac tactccaatt atgcctcgag gagcatttcc acccgatgtt
      841 ggtactagcg gacaccttct tgtactagca ggaggaggaa gctataaatc acagccattt
      901 agtggtgcgg gatggcgttt tggaggtatg ggtgtaaaac cagctgatgg tacaggcaac
      961 tttttacgtt ggaaatccta cattgacgat ggaagagtag gaaatgtaaa tcaagccaac
     1021 gcaactaaat ctggttctat tcaaggaatg gcattgtatg ctcgtgaagg attcaaatac
     1081 tttgcaccaa acgggcctgg ttggacaatg aatataggta tttattacac ttataatcct
     1141 gttgcaggta cgtattatgt tgttcaaaat atttaataaa acagcacaaa gagatttctt
     1201 tgtgctgttt tattattttt agttgttgac aaacactaaa atataaaata taatcaacac
     1261 ataaacgaac gtatgttcgg caagaaagca ggggattttt atggatgaga tggttttaag
     1321 tactcagaaa tggctaaaca agaaatacag taatgttaca gggtttgata aggttccaga
     1381 aaatgggcga acagggtggc ctacaatcta tggacttatt gaaggactac aagtagaact
     1441 tggtattact aatctagtag ctaattttgg acctaccact gaaaaaatgt acgataatca
     1501 ggttactcct aaatggggca aaaatctacc taaaaatatt gtatttctta ttcaaggtgc
     1561 cttttggtgt aaggggatta atccaggtgg cttcgatggg gtatatactc catatttaga
     1621 tactgctgta aaagagttgc agactgatgc agggtttaat ggaagcgctg taactggagt
     1681 tttggatgcg aaatgggcca aagcgttatt tgatatgagt gcttttgttc ttgttcccgg
     1741 cggagatagt aaaattagaa aaatgcagca atggttaaat gtaaattatt tagaatacac
     1801 gggaataatg ccttgtgatg gtatttatca aagaaattca aatcaagcat taatttttgc
     1861 attacaagct gaactaggat attctcccag tgaagcaaca gggtcttatg gtaatgggac
     1921 aactagtaaa acttatcctg tgtctgaagg taactcggga aactatgtaa gaataattca
     1981 atatggatta tatgttaatg gttattttga agacggtaca ttcgatggta ttttcactaa
     2041 atatatgggt ttagaagtac tggcattccg aaaatttatg gtattgcctg aatatactga
     2101 aatagcagat gttgttgtaa taaaaggact attgtctagc gctggagata ttagaagatc
     2161 tgcagatggc gcagatactt ctacgcaatt aactagatct caaatccaaa cattggttga
     2221 taatgatata aaaatagttg gtagatatct tactggtact gttggaattg gaaaagatga
     2281 acgaaataag tatctaacta cagaggaatt gaataatatt ttttctaaaa atttatctgt
     2341 gtttcctatc tatcaagatg gaggtgcagc attagcttat tttacttatg atcgtggctt
     2401 atcagatggt aagaaggcta tagatgcagc aaaaaatcta aatataccat taacaacagt
     2461 tatttatttt gctgttgatc tagacatgtt aggggaagaa attcttgcat atgctggaga
     2521 atattttaag ggggtatctg ctgtaatgtc atatagcggg tatcaaacgg gtgtttacgg
     2581 aacaagaaat gtttgttctc aaataatcaa taacgggtat gcttcgtttg catttgtttc
     2641 agatatgtcg actggttata gtgggaattt aggttttcct atgcctagac aatggtcttt
     2701 tgatcaattt gttgaattta ccataggaag tggcaacgga gctgttggta tcgatttaat
     2761 cgctacatcg ggtagagata gcggatttaa tgagttatca aatgatacta ataatgataa
     2821 ttacattgcc aaatacaatc agaaggttat aaatcagatg gtacgtgcgt atcaatatct
     2881 atcacaagct ggtcttgata atccttggaa ccctcatttg acattttata gatatgtaaa
     2941 ttattctggt ttgtcatggg acatcatttc ttctcctgta acagagcatg atagaaaaat
     3001 ttatgatgaa tataggaata aattaactaa tagcgagggc ctgtataatt attttataga
     3061 tcctaatact ggttcaataa taggtttgcc tcacttaatt gtgacgttac aatcacagat
     3121 gtttctagcc gacacaatca atgatagtgt ttctgacttt gcaggttggt taggagattt
     3181 aatgacttgc tggggtgaag ttaagaaatt aggaatacaa atggagcaag gtgtatttgc
     3241 attagtaggt acggctgcag gtggaacatt tagtctagaa gatttatacc aagatgttga
     3301 cgcggtaaat gtttatacct tagcaaaaag aaattctgat tcaacagctt tatcggcttt
     3361 aaaaacgtac tattgtgata agggtgctat tgatagattt aatcaatttg ttaagaacag
     3421 atttggttct tcagataaaa tcgcttcagt aacaaatagt ttattagaaa atgacgaaac
     3481 agttgatatc ttaggtgtag ggataatttc atttttcatt gataaaatag ttaataattc
     3541 acttgggaca gattttactt atcttgattt tattggagat gaggggaaag ttgcaaatgg
     3601 attttctgaa aaaattattc aatttcaaga aaatagaagt aaacttaatt aagaatcgtt
     3661 tactatttaa acagtgtcct tttccttttt aaatatatac gataaaataa gtatatgaat
     3721 tgtaaaagga catgatgatt atggaagaaa tagaatggaa taatctgact caaaataata
     3781 aaaataattc tggatgtgga attatcttta tgtttgttat agttttggga atagtagtac
     3841 ttttaataac tgtcaagata gtagattact ttgcgaatac tcctgaagaa caaatacaga
     3901 gaaaacttcc tgagtttgaa agagtaccac cttatgatcc aagagataat tcatttcaag
     3961 taaaaaaatt aaaagagtct ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg
     4021 acttcaaaga aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat
     4081 ttatttttaa ccctaaatta gaaatatata gtgttgtaat agaataatag tgttaaaaaa
     4141 attgtaaaag ttaaagaagt caaattctga tgtgaaaaat aaaatatacc atggaagaca
     4201 taaaagaaaa a
//
LOCUS       DACDNO010000130         4156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDNO010000130 DACDNO010000000
VERSION     DACDNO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.132"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            178..774
                     /locus_tag="IWI22_002794"
     CDS             178..774
                     /locus_tag="IWI22_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9170111.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IWI22_002795"
     CDS             898..2697
                     /locus_tag="IWI22_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9170112.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="IWI22_002796"
     CDS             complement(2975..3187)
                     /locus_tag="IWI22_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170113.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(4051..>4156)
                     /locus_tag="IWI22_002797"
     CDS             complement(4051..>4156)
                     /locus_tag="IWI22_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9170114.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1228 a          706 c          729 g         1493 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctataatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagcagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat atttaatata atcttaattg tcaaattaag ttagacagat catcaatact
     4081 tacgtaactc aaaaatactt ccaaaggtgt tcgataatcc agtgattttc taggaatatt
     4141 atttcgttta gatgcg
//
LOCUS       DACDNO010000131         4028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDNO010000131 DACDNO010000000
VERSION     DACDNO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.134"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            392..1432
                     /locus_tag="IWI22_002798"
     CDS             392..1432
                     /locus_tag="IWI22_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9170115.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2256..2588
                     /locus_tag="IWI22_002799"
     CDS             2256..2588
                     /locus_tag="IWI22_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9170116.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            3207..3548
                     /locus_tag="IWI22_002800"
     CDS             3207..3548
                     /locus_tag="IWI22_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9170117.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     NEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            3567..3857
                     /locus_tag="IWI22_002801"
     CDS             3567..3857
                     /locus_tag="IWI22_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170118.1"
                     /translation="MKFFEVKSPYYYALIAAKNEEECAELYEQVVTDIENREEFIRCL
                     HELNRNEAIQEDAKKISEETLQPIGMEEATKDIFTIIDEQKSCVLSIDSTLV"
BASE COUNT         1511 a          526 c          738 g         1253 t
ORIGIN      
        1 aacctaaagc cttgtcttat aagaataata taatatatac tactgaaata aggtagtata
       61 tattgctagt aatggtagta tgttttatat gcataaggta gttcttaatt tattttttgt
      121 tttgacactg gtagtactta attatcttta attttaaaac taaattttat tcatacgttc
      181 ttctggtagt atttaattat aaataatatt tatttaaagt agttcttaat gactttaaag
      241 gtagtacata attcatttta tttctgattt taggtagtat ttaattaaaa aacagtttta
      301 atgcgtttgc attttttatt tatctttgct attttcgaaa agagtaggta attttacata
      361 aataaaacaa cgtgaaagta gaggatctac aatgactgat tttaaatttt ttaaggcgga
      421 tagagtttac aacgaattat tttatcaatt tccaaaagtc tttattgttt ctgacgaata
      481 caaaaaaatg aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa gattagagat
      541 cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt tattttactt atacgacaaa
      601 tgaactatgt agagtattaa actgccaaaa acaaaaagcg atagcaatca aaaaagagtt
      661 ggaatccttt ggtttattat tacaaaagca gatgggattt aacaaacagt tagggaaaaa
      721 taatcctaat agactatatc tagcagaatt aaaagtctca gaaaatgata tctacttact
      781 cgaaaaattt gatagagaga atagggaaaa cgttgataaa tcagagggta tgaaaatcat
      841 acccaccctc gacgaaaaat cagacgctga atcccttggg gctcaagagg gtatgaaaat
      901 cataccgtgc caaaacgttg ataaatcaga gggtatgaaa atcataccag aacttaataa
      961 taatatatta gacactaata gacacaatat agacactgaa aaagaccgcc tacaagatca
     1021 attgttgtta gacaattttg agacaattat gacaaacgac agcattgcta cgtttgtccc
     1081 tgaacgagta ttaaatttga taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac
     1141 cgtccagact attcataatg caaagaaaaa agctgaaata gaaagtggta tttcgatagt
     1201 ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa gaattataca cgacactgtt
     1261 aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt
     1321 ctatgtaaaa aattggttta tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg
     1381 tttgaattat gaaagctccc caagcactat tacgaaagac tggttagagt gaaactttac
     1441 tgacaaagtg attgttgctt catgaagtaa cattaaaatt aaaaattttt tattattcta
     1501 attatattac gaatattaca aaataatagc tattttaata gaatttataa gttaaaatat
     1561 tatgataaaa atcacaaacg tagcctttta cgtcataagt gttaaaagaa ataattaatt
     1621 tcaaatatag aaaagggaac gtatgtttgc atactccttt tttttgagtt ataataatac
     1681 gcgaagagag ataatttcat tatttaaaca agctaaaaat cttgatatta cttcttttca
     1741 ggcaatacga atattattcg cttacatttt ttgtataaat ttaattgtct gctatgcttg
     1801 tgatgaattg attttcgttc tgcacttttg gatgataaat ttggacaatt gaaaatacaa
     1861 aaaaaagaaa ggcggtatga actcggaagg ctggtaacct tcgtcagcgt tcatacacgg
     1921 cattaagatt tggcccttaa tgcgttaacc tttcagtgtt acggtattac ccgcaatgga
     1981 tttagtataa cagatattca aaagtgatac cacttttttt ggctgtgtta aggttaaaaa
     2041 tacgtgatat tacgatgaat ctatgcctaa gcataatgtt ataaaaatgt ccgtgtggct
     2101 ctttgccgta tgacgaacgc tgaaattaga gggaacgcat gtttggatgc gttccctctt
     2161 ttttgtttgt catgaacgaa aatcaattct ctatttttga aaaataacaa gaaaaacttg
     2221 ttaaaaatat ttaaagaaat tgggtggttt attttatgtc atatacagga attttgagtc
     2281 tcaaagatat ttgtcactac ggtaaacggt gtaccgctac agagaaaatc acgaagaaac
     2341 tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa atatataatc cagaaagaca
     2401 aagtctcaga ggaaatgatt tattacattg ggaaacaaaa gcaaataatt ttaaaagatc
     2461 ctatcccctt aaaagagcta tatccaacaa tcaaacatgt ttatgaccaa aacggtgtac
     2521 tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc aaaagggatg gggcgtttaa
     2581 tcagctgaat tgatcattct cataacttgt attttatcgt ttttcgcttg cttgaaaata
     2641 ttgtcgagac ttctaaaaga acttagaatc attagaaaaa taaggagtag acttgatcta
     2701 tcaacaattt ccttcgggga gtgacggggt agatttttac gattggtaac gtaaaaaatt
     2761 ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa tgaatttgtg taaataatat
     2821 acacagagta aacaagaggg aatttagagg ttagacaagt tagaatagcg cctagattaa
     2881 tcatgagcag ctactaaggg atctaggctt tgacgcaaac tcatggaata aggagtaggt
     2941 cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg tgctgcagta catgtagata
     3001 cttgcgagga cgttggtcat tggatcaatc gagcatttac cgctgtctac tagggtttct
     3061 cgtttcggac gccataaacg agtcgatatt ttttatgtat ttttaagtaa acgaaaaacg
     3121 ataaaatatg aacgagtcct gaataaagtg aaaaaattga tataaaacat agagaaaatg
     3181 aaatttacgt aatggaggaa tgacaaatga aaaattatga tccgaatata cgattgggca
     3241 cacatactat taaggtttct ttccaacgtt gggattacaa aggttttgtg acgtttagaa
     3301 gaggcggaaa ttgtaaagga ttagatgttt tagcattaaa cgaggatgat ttatacgatc
     3361 aaacattgac tgataatcca attggattcg gcttgctacc tgaagatgat gaaggtaatg
     3421 aatggtttaa aatgacgctt atgaatgata atggagacga attatctgtt gaagatactt
     3481 ggtcatattt gagtgattat attgtcagcg ttgaaatcat tgaatttgta gcggataaag
     3541 aagaataaaa actggaggaa taaattatga aatttttcga agtaaaatca ccatactatt
     3601 atgcgttaat cgcagcaaag aatgaagaag aatgtgcaga attgtatgaa caagtagtaa
     3661 cagatatcga aaatcgtgaa gaatttattc gatgtttgca tgagttaaat cgaaatgaag
     3721 caattcaaga ggatgcgaaa aaaattagtg aagaaacgtt acaaccaatc ggtatggagg
     3781 aagctacaaa agacattttt acaattattg atgaacaaaa atcttgtgta ttaagcattg
     3841 attctacatt ggtttaatag atccaagatt ctataatttt tttcaaagta aaaagaaatt
     3901 aggtaatgat tctcagacca attacttagg aaaataacca tacaaattag attttacaga
     3961 taaaggatta gaaggaatca cggcgattaa taaaaaggta gtattacctt aggcattgag
     4021 taaggaat
//
LOCUS       DACDNO010000132         3884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDNO010000132 DACDNO010000000
VERSION     DACDNO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.135"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(87..902)
                     /locus_tag="IWI22_002802"
     CDS             complement(87..902)
                     /locus_tag="IWI22_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9170119.1"
                     /translation="MMKSNVSKLSSKDLRKVSLRYMFGAQLGWNYERMMNVAYVRAVY
                     PALKKLYKDEKELKSVLQSEMQFYNTSPFLSAFIIGIDLALQVEDGSKSKDAVAAIKT
                     SMMGPFAAVGDSLFGAVIPTIFGSLAAYMGLEGNPLGVLLWLLVSLIILFLRYFELPI
                     AFREGKKLVSSVGGLLKDLTESATLLGVVVIGGLVPSVVKVGVPFKLVIGDKKLSFQT
                     EMLDPIMPAIVPVVLVLLAYWLLSKKSLNSTKVIWIFLVLSILMYSLNILAVL"
     gene            complement(889..1701)
                     /locus_tag="IWI22_002803"
     CDS             complement(889..1701)
                     /locus_tag="IWI22_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9170120.1"
                     /translation="MNDISFFQIFSLICLAILGGIDPYLSGLQLSKPAISGFLAGIIM
                     GDINTGLLVGATLQLMVLGVGTFGGSSIPDFGTGAIIGTALGVVSGKGIEFAIGISVP
                     VGLLLVQLDILARFCGVYFLHRVDKHIEEENFHKISLEAWLSLVPIALSRAIPVGLCL
                     IFGNEVVNTVLNYAPDWLMGGLKLAGAILPVVGIAILLHYLPIRNFFGFLIIGYILAA
                     YLNIPMMGISFAGLAAAIIHYKKLQDRRLLESKLDTINHIEVVNGEVDDDEI"
     gene            2003..2128
                     /locus_tag="IWI22_002804"
                     /pseudo
     CDS             2003..2128
                     /locus_tag="IWI22_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2159..2566)
                     /locus_tag="IWI22_002805"
     CDS             complement(2159..2566)
                     /locus_tag="IWI22_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9170121.1"
                     /translation="MAGHLVLISHGRFCEELKRSVDMIMGVQKDIYTVGLLPEESAKD
                     FRVNFEKTINGLKKVTVLADLMGGTPCNVVSQLILEGKEIELYAGMNMPMVIGFVNNQ
                     FLGEDSSLVNKGRENIIHVNEYLEVNPIDLEDE"
     gene            complement(2589..3047)
                     /locus_tag="IWI22_002806"
     CDS             complement(2589..3047)
                     /locus_tag="IWI22_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9170122.1"
                     /translation="MVSSSLHGQVANLWTTKLNISRIMVVDDEVAQNDIEKSGLKLAT
                     PAGVKLSILPIEKAAQNILAGKYDSQRLLIIARKPDRLLKLVELGVPIKEINVGNMSQ
                     TSETRSITKSINVVDQDVETFNQLNEKGVHLIAQMVPSDKADDFMSLLNK"
     gene            complement(3192..>3884)
                     /gene="tnpB"
                     /locus_tag="IWI22_002807"
     CDS             complement(3192..>3884)
                     /gene="tnpB"
                     /locus_tag="IWI22_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9170123.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT         1275 a          785 c          504 g         1320 t
ORIGIN      
        1 cttctttcca ctcatttaaa aatataatat ttagaaaatt aaacagggga catgccataa
       61 attagttgat cacgccccca catctattac aaaactgcta aaatatttaa tgaatacatt
      121 agtattgaca aaactaaaaa tatccaaata actttggttg aattgagaga ttttttgctt
      181 aataaccaat atgccaataa aactaaaaca acaggtacta tagctggcat aatagggtct
      241 aacatttctg tttggaaaga tagtttctta tcaccaataa ctaacttgaa tggaacacca
      301 acctttacaa cactaggaac caagcctcct attactacta cgccaagtaa agttgctgat
      361 tcagttaaat ccttcaacaa tccacctaca ctggatacca gttttttacc ttctcggaaa
      421 gcaattggta actcaaaata tctcaaaaat agtataatga gagatactaa taaccaaagt
      481 agtacaccta atggatttcc ttctaatccc atataagctg ctagagatcc aaaaattgtt
      541 ggaatcactg ccccaaataa agaatcgcct acagctgcaa aaggtcccat catactcgtt
      601 ttaattgctg caacagcatc tttcgatttg cttccatctt ctacttgcaa tgctaaatca
      661 attccaataa taaatgcact taaaaaagga ctggtattat agaattgcat ttctgattgc
      721 aacacacttt ttaattcttt ttcatctttg tataactttt tcaaagctgg atatacagct
      781 cgaacatatg ccacattcat cattcgttcg tagttccaac ctagttgagc tccaaacata
      841 tatcttaaac taacttttct cagatctttg ctacttaatt tgctaacatt agatttcatc
      901 atcatcaacc tctccgttaa ctacttctat atgattgata gtatctagtt ttgattctaa
      961 taaacgtcta tcttgtaatt ttttatagtg aataatagca gctgccaatc ctgcaaaaga
     1021 aatacccatc attggaatat ttaaataagc ggctaaaata tacccaataa ttaaaaaacc
     1081 gaaaaaattc ctaattggca aataatgtag taaaatggca atcccaacaa ctggtaatat
     1141 agctcctgcc aattttaatc ctcccattaa ccaatccggt gcataattta atactgtatt
     1201 tactacttca ttaccaaaaa ttaaacataa acctactggt attgcacgag ataatgcaat
     1261 aggaactaaa cttaaccatg cttctaaact aatcttatga aaattttctt cttctatatg
     1321 tttatccaca cgatgtaaaa aataaacccc acaaaaacga gctaaaatat ctaattgaac
     1381 taataataag cctacaggaa cagaaatacc tattgcaaat tcaattcctt ttccactaac
     1441 aacacctaat gctgtaccaa tgattgcccc tgtgccaaaa tcaggaattg atgagccacc
     1501 aaatgttcct acacctaaga ccatcagttg taatgttgct cctactaata agcctgtatt
     1561 aatatctccc attataattc ctgctagaaa accagaaatt gccggtttac ttaattgtaa
     1621 tcctgacaga taaggatcta tacctcctag tatagctaaa catattaaag aaaatatctg
     1681 aaaaaaagaa atatcattca tgatcaaact aactccttta tattattttg aacttttcac
     1741 tgccccaata tctagtatgc catctaactt aataccaaaa aaatcagttc ctaaattata
     1801 accattttta tcgataacct tcttcccact atgaatagca ggagatgatt gttccaattt
     1861 atatccatca aacactgtat tctgactgaa tcgatcatat gtcaaatagt taaaattagg
     1921 ttttacggga ccattttcag gagaaacaaa tatattcgac tcttggctat tactgatttc
     1981 aatacacgcc tctatatttt ctgaattttt tattcaatct gtcgcatcta aacgaaataa
     2041 tattcctaga aaatcactgg attatcgaac acctttggaa gtatttttga gttacgtaag
     2101 tattgatgat ctgtctaact taatttgaca attaagatat tcataaattc ctccacattc
     2161 attcatcttc taaatctatt ggatttactt ccaaatattc atttacatgt ataatatttt
     2221 ctcttccttt atttaccaaa gaactatctt ctcctaaaaa ttgattatta acaaagccta
     2281 taaccattgg catattcatt cctgcatata gttctatttc ttttccttct aaaattaatt
     2341 gagaaacaac attgcaagga gtgcctccca ttagatcagc cagaactgta acttttttca
     2401 aaccattaat tgttttttcg aaatttactc gaaaatcttt tgctgactct tcaggtaata
     2461 accctacagt ataaatatct ttttgtactc ccatgatcat atcaacactt ctctttaact
     2521 cttcacaaaa tcgtccatga ctaattaaaa ctaaatgccc cgccatcttt tcacctcctc
     2581 gtatattatt atttatttaa cagagacata aagtcatctg ctttgtcact tggaaccatt
     2641 tgcgcaatta aatggacacc tttttcattt aattgattga acgtttctac atcttgatcg
     2701 acaacattaa ttgatttcgt aattgatcga gtctcagaag tttgtgacat atttccaaca
     2761 ttaatctctt ttattggaac gcccaattct accaatttta ataatcgatc aggttttcgt
     2821 gcaataatca gtaaacgttg tgaatcatat tttcctgcta aaatgttttg cgccgctttt
     2881 tcaatcggca aaatacttag tttcacccct gctggggtag ccaatttcaa tccgcttttt
     2941 tcaatatcat tttgagccac ttcatcatcc acaaccataa ttcgagaaat attcaatttg
     3001 gttgtccata aattggctac ctgtccgtga agtgaactac tcaccactta gctaaaccta
     3061 acggtttctt acgcttgaag tgggagcttc ttgggaagag agcatacttg atagcgtatt
     3121 actttactaa cagaggtgcc tcttatttac caagctatcc ccgtagttcc tacggttctt
     3181 gtttatttga tctaagccaa tcccatagag tgtagcctta gaccttcggt caggatattg
     3241 atactagcgt tgatatctcg gtcatgatga gtatgacaaa tagggcaagt ccattctcga
     3301 atttcgagag atttcttgcc gtctttatgt ccacaattcg aacagatttg gctagatgga
     3361 aaccacttat ctactttgat gattttccga ccgtaccagt cagccttgta ttgtaattta
     3421 gacacaaaac tagaccaaga aacatccgaa atactttttg ctagtttatg attacgcaac
     3481 atgccttttg tgtttaagtc ttcaatacag atgatatcgt gatttttgat aatttctgta
     3541 ctcaacttat tcagaaaatc agtacgttga ttcattactt tttcatgcaa tcgtgctact
     3601 ttacgttttt gtgtttgata atttctagct tcaaacagat tgataccttt ttgtttagct
     3661 aataaggcac gtcttgacaa cttacgttgt tcacgtttga gtttcttttc cattttggac
     3721 gtaaatttat tattatcaaa tttttgaccg gtagaaagaa ttgcaaagtc tataatacct
     3781 aggtcaatac caacagcaga attagattta ggtagttcgc gaacttcttc tttgcataac
     3841 aaagaaatat aatatttacc gctagaatga cgtgatattg tagc
//
LOCUS       DACDNO010000133         3484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDNO010000133 DACDNO010000000
VERSION     DACDNO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.139"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1740)
                     /gene="uidA"
                     /locus_tag="IWI22_002808"
     CDS             complement(<1..1740)
                     /gene="uidA"
                     /locus_tag="IWI22_002808"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9170124.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRK"
     gene            complement(1763..>3484)
                     /locus_tag="IWI22_002809"
     CDS             complement(1763..>3484)
                     /locus_tag="IWI22_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9170125.1"
                     /translation="EDAISWIEETIDSMTLEEKIGQLFVNMGSSRDKNYLSNMVNQYH
                     IGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTDGTEVGVEVKIGAT
                     NDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRSFGQNPDKVLELSL
                     AYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWDNSFGKVYSGLIEAGL
                     PSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGKLGFNGLVVTDASHML
                     GMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKGIITDDRLLDALKRIL
                     GLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQAITLVKDKQDIWPIVP
                     EKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVEKYISPEEELKGLS
                     QEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIVWPASKGTPDIPFYIH
                     EIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGLSEFKGVSPIDAYC
                     GLKDTFI"
BASE COUNT         1078 a          723 c          435 g         1248 t
ORIGIN      
        1 ttttctatct ctagtaaaga ttcctttctt atttccttgc actcttaaaa tcccttgact
       61 tgttgcaaaa tctgcaaaat tccacacttg ttcaccaaca aaattttcac atgtatctat
      121 tacttcgtga tttgcttcgt aataagccac ttgatactct tctgtaaaca ttgttggtac
      181 tgtatcatga aatccagcta ctgtatctgc tccatattct gtaaacatta ttggcgtatc
      241 aggacatctt tccatccatc catctagctc ttttttcaac agatattttg catgtgccag
      301 atcccctccc gaaacatacc aaccataata acgatttaaa cataaaacat cgacaatatc
      361 tcctacctta caaacatctg gcgttgattt tagataagta acgattgtta caggtctttt
      421 ctgcggatca agttctttag ttaattcaac taaaggttca aaatattctt tagcaccttc
      481 ggaatccgaa tctggttcat ttgcaataga ccacattaca acacttgggt ggttcttgtc
      541 gcgttctatc aattccttga tcaccgtttt atgagaattt tctgttcgta tctctttcca
      601 cgtattatgt ttttccacat tatcatctaa taaagtggcc atgaaattta aatgtaaccc
      661 tactgcaggt acttcatcaa taactacaat accttctcga tctgctaatc gcataatttc
      721 ttctgagtaa ggataatgtg atgttctaaa agaattagca cctatccatt tcattaaatt
      781 aaaatcttta atatttacag cctcattcaa accacgtcca ttaacatacg aatcctcatg
      841 cttaccaaat cctttaaaat aaaaaggttt attattaatc aaaaatttgc cattggtaac
      901 ctgaacactg cgaataccaa attcttctgt ataggtatcg atcactttat tatcatcaat
      961 taaactaatt ttcatactat ataaataagc gtctagaggt tcccaaaaaa ttggattctt
     1021 aagctgaatc attcctttct ttccttcagc ttcagctaca agttgagcct cttcgtcata
     1081 tatcgctatt aagatttttc cggtccattc tgtatcgata ttgaattcta cacttgcatc
     1141 caaatcagtt attcttgtat taatagtaat atcacttata tagtctttcg gagttgtata
     1201 taacttaaca ggtctatgaa tacctgcata attgaagaaa tcaaaattaa ctaagttttc
     1261 cttttttata tccccatttt cctgtactgt ttccttcata atccccacag gtaaagtact
     1321 atcgtcaata atattgttta cagcaactgt aattcgatta ttcccgtctt ttacaaaccg
     1381 attaatttca gactcaaatg gtgtaaaccc acctttgtgt tgagtaataa actttccatt
     1441 caaaaaaact tttgcttcat gtgttgcaga tccaaaacga agaactaatc tttcttctaa
     1501 taatctttta ggaagagtca cttctttttc ataccacacc catccaacat gatttctaat
     1561 gttctctgtt tcaaataaat cattataaga agagggaact gaaatcggat aactctcttt
     1621 ttcatttatt ggttctaaag caagttcttc ctgtaagtca tcatttgtat ctaatttaaa
     1681 tctccagaga ccgcttaagt caattacttg cctcgtctct gtcataatgg gatacaacat
     1741 ataaaccact cctatctaaa tattaaataa atgtatcctt taaaccacaa taagcatcta
     1801 ttggacttac tcccttaaat tcagaaagac ccattagttt gtccaccaat atttctaaag
     1861 tttcttgttt tccatcataa gtattaatat aagtctttac ttgaggtaca tcagccaaat
     1921 gaaacggaca ctgtacagat acaaaaattg ttggaatttc atgaatgtaa aacggtatat
     1981 ccggagttcc tttacttgct ggccagacaa ttcgttgaac tgtgcctgga ttaacattag
     2041 ctatattaat gatcaagtca tattttttag ttaaattttt aattggtctc ttagcagcat
     2101 atattccagc tatattttta attttttctt cttgcgataa tcctttcaat tcttcttcag
     2161 gtgaaatgta tttttcaact tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa
     2221 tggcttgagg tctatttcca gcaactactt caccaaaccc accttttacc ccatgtacat
     2281 ctactagtaa aatcctttta tatttttctg gtacaatagg ccaaatatct tgtttatctt
     2341 taactaatgt aattgcttgg tcagccacct ctttaaaaat tgatttattt tcaattaaac
     2401 caattttttc cagagcttct tgcttaggta ataaaatttg ttcttttgct tttttgtgta
     2461 atcccaattt agcttttaac cctaaaattc tttttaaggc atctaataac cgatcatcag
     2521 tgataattcc tttacgatag ccttccatca tataattaaa gtcttcatct ggatcattaa
     2581 aaaatagaaa tagatcacat ccagctgcaa tagaagtggg caataattct gatcgtttca
     2641 ttgccccagt cattcctaac atatgactag catcagtaac tactaatcca ttaaatccga
     2701 gttttcctct aagtaagtca gtgattaatt cttttgataa agttgcagga agtaaatctt
     2761 catctttcag atcaggatta aaatgttttg aataagctgg catatggata tgacctgcca
     2821 taattgaagg taatccagct tctattaagc ctgagtatac ttttccaaaa gaattatccc
     2881 attcttccgt acctaaacta ttaatactaa aagatagatg ctgatctcgt tcgtcaatac
     2941 cgtcaccagg gaaatgttta gctgcaggca aaattccact ctcctgaata cctttcatat
     3001 aagctaaact aagttctaaa actttatcag ggttttgtcc aaaagatcga ctagaaatga
     3061 taggatttct ccaatttcta ttaatatcta caataggagc aaaactccag ttacatccaa
     3121 tagccgatgc ttcaatccca gaaactctcc ctaattcata agcatatttc gcatcatttg
     3181 ttgcaccaat ttttacttct acaccaactt cagtaccatc tgtacaagct ccgttaccac
     3241 ctgcttccgt attagcagca attaatagag gaattttact ttctgtttgt aagatatagt
     3301 tttgatcata tacctcatca gcagttcctg gattatatcg tacagctcca atatggtact
     3361 gattgaccat attactcaag taatttttat cacgactaga tcccatatta acaaacaatt
     3421 gacctatttt ttcttccaaa gtcatagaat caatcgtctc ttcaatccaa gaaatagcat
     3481 cttc
//
LOCUS       DACDNO010000134         3280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDNO010000134 DACDNO010000000
VERSION     DACDNO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.140"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..584
                     /locus_tag="IWI22_002810"
     CDS             <1..584
                     /locus_tag="IWI22_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9170126.1"
                     /translation="AFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELS
                     KKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMAN
                     SGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVL
                     EGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(661..1836)
                     /locus_tag="IWI22_002811"
     CDS             complement(661..1836)
                     /locus_tag="IWI22_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9170127.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1918..2298
                     /locus_tag="IWI22_002812"
     CDS             1918..2298
                     /locus_tag="IWI22_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9170128.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2372..2734
                     /locus_tag="IWI22_002813"
     CDS             2372..2734
                     /locus_tag="IWI22_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170129.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1113 a          538 c          622 g         1007 t
ORIGIN      
        1 tggcattccc acaactagat ttaaaaagtg aaaaaggacc aaaagctgtt atcaaaacaa
       61 accgtggtga cattactgtt caattattcc ctgaactagc accgaagacg gttcaaaatt
      121 tcattgaact ttccaaaaaa ggttattatg atggcgtgat ttttcaccgc gttattcctg
      181 atttcatgat ccaaggcggc gatccaactg gaacaggaat gggtggcgaa agtatttatg
      241 gcgaaagttt tgaagatgaa ttcagtcgtg aactgttcaa ccttcgcgga gctttatcta
      301 tggcaaattc aggtccaaac acaaacggaa gccaattctt tattgtaaat aacacaaatg
      361 tgccagctaa tatgcttgga caactagaag gtgctggatt cccatcagaa atcattgaag
      421 cgtataaagg tggcggaact ccttggctag atttccgaca tacagtcttt ggacatgtac
      481 tagaagggat ggacacagta gatgaaatcg caaatgtaca acgtggtcca caagaccgac
      541 ctgtacatga tgtagtgatt gaaacaattg aaatttcaga atagtttggt atggtacgga
      601 gtaattatga aaaaggacga aacctagtaa ggtctcgtcc ttttattttg aataaatgat
      661 ttaatcgatt gtctctaaag catacttgat tcgattaagt ccttccagta aagtttcttc
      721 tggacaagca atattcaagc gcatatgccc atgaccggaa ggaccaaacg tgatgccagg
      781 atttaatacg acttttcctt tccgaacaag agtgttctcc aattcttggt cgtctgaaat
      841 ataggcggta aaatctaacc acattaagta agttccttca gggttcatta ttctcacttt
      901 cggaagatac tgttcaaaaa actgattaac tgtttgaaca tttttttcta gataaggaat
      961 cagttggctt aaccattctc cacccgtctc ataagccgct tgggttccaa tcagtccaaa
     1021 agtgttgatt tcgtgctgtt ggttcatcac taaatgtttt tcaaaatcag ctttcatctc
     1081 tggatttttg ataaatacca ttgaattttt gattgcagct agattgaaag tcttggttgc
     1141 ggaagtaaaa gcaactagta gatcatctaa attaggatca atcgttagca tggaagtaaa
     1201 tgtatgatcg aataaagtta aatcttgatg gatctcgtca ctaaacagaa tcacttgatg
     1261 ttttaaacac aattcgctta actgtttcaa ttcttctttg ctccaaacac gccctccagg
     1321 attgtgagga ttgcataaaa tcattgcttt tacattgttt tcttcgattt ttttctccat
     1381 gtcagacaag tccatgatga aatgattatt tttttctagc aaagagcttc gaacgatgtg
     1441 acgttgattt gtttctataa tagaggaaaa tggaggataa actgggtcat gaatcaaaat
     1501 agaatcgcct ggttttgtaa aagtttgtac tgctgcagct aaactggtta acacaccact
     1561 tgtaaaaacg atattttctt tcgtcaactc cacagcatgg tgttggcgtt cccaatgaat
     1621 gatagcctga tatagtttat cagacagagt ggcataacca aagattccat gtttgatgta
     1681 ttcactaaac gctttagcta ccccaccggg agacaagaag tccatatctg ctacccacaa
     1741 aggaagtaag tcatccattt gatatgtctc agcaattgcg tcccatttta cactttctgt
     1801 atcttttctt gaaatctgtt tatcaaattc gatcatctta agacctccag tcattttcgt
     1861 cgcttctttt tttgagctat ttacagtata atgataaatg cgaagagagg gaagtagatg
     1921 acttataaaa ttggacaaat aattgaagga aaagtgacag ggatacagcc ttatggcgca
     1981 ttcgtttctc ttgataacga gacgcaagga cttattcatg tttcagaagt acaatccggt
     2041 tatacgaaaa atatccattc attgttaaaa gtcggacagc cagtaacagt tcagatcatc
     2101 gacattgatg aatattccaa aaaaatcagt ttatctttaa gaacgttaga aaaaacaaat
     2161 ccagctgttc catatcgcag aaaaaggtat ttcacgaata aaaatcgtaa gatcggattt
     2221 accactattg cggagcagtt acctatttgg atagaagaag cattatctga actagaacgg
     2281 aaagaaaata agaaataaaa agtgtatttt ttctgaaaat gtgctagaat ttttatgtaa
     2341 gccatagaac aagttgatcg aggtggaaat tttggcaagc aaaagagttg ctggtacaat
     2401 tatgttgcat ttggaggatg gtactaaaaa atttttggtc cattcaattg atgacaagtt
     2461 ggaatttgcg ttagcggaac tttctgaagg aaagactggt ttagcgaata tacttaattt
     2521 cttaaaagaa atagttcata tcgatgttag caagattagt ttgatggaat tgacaaatac
     2581 acatattaaa gaggaaaatg ttccgttgtt tgtctttgaa actgaacaaa agaatcaaag
     2641 agaagaatta ggcgaaggat atatttggga agaaccaggt caaatgcgct ctgttcttgg
     2701 aacttacgaa gtagagggag tgcctttctt ctaaaataca gattaagcga acaaagaagt
     2761 ttgaattttt ttcaaacttc tttttttatt gtttggtcca aaaaatacaa tgaaatgttc
     2821 tgctaaagat agttttttag tgcattttat tttcaaaata tgttgaaaac ggttgtataa
     2881 cgaataataa taattgatcg aaaaatcatt tcacaaagta atgcttaatt tatagttgac
     2941 caaggaagaa atacatgata aatttaacag cgttgattct gaagtggatg ttatatcaca
     3001 tttctaaaaa atttaaaaaa tagttattga catgttacta acgacatgat aagataactg
     3061 agtcgttaaa aaagaaccac ttgcgaagct tgattctatc gaaaaaaact tcaaaaaagt
     3121 tcttgacaaa taacaaacga taagttatga taaatgagtt gctgaaagat ttcgaaaaac
     3181 gaaatcgaaa aaaactttta aaaaagagtt gacaatcgct tgtctctctg atatgatata
     3241 aaagttgcta cggcagccaa aaacgaatcg ggaaacactc
//
LOCUS       DACDNO010000135         3271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDNO010000135 DACDNO010000000
VERSION     DACDNO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.141"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            77..1825
                     /locus_tag="IWI22_002814"
                     /pseudo
     CDS             77..1825
                     /locus_tag="IWI22_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333370.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
     gene            1839..2408
                     /locus_tag="IWI22_002815"
     CDS             1839..2408
                     /locus_tag="IWI22_002815"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAP9170130.1"
                     /translation="MKIYTKSGDKGNTSTSIIGGERIKKSDARVCAYGSVDEVNSWVG
                     KIISELDDKKFELLKKELVVLQILLFDIGTDLATPIGIKEMIVGKEEVTKIETLIDFY
                     QAKVPVIEKFILPGGHPVACDMQITRTVIRRAEREITRLIVSDEPINLHAYKIMNRLS
                     DLFFVLARYVNHVYATKEPFYERAGKVFH"
     gene            2424..2828
                     /locus_tag="IWI22_002816"
     CDS             2424..2828
                     /locus_tag="IWI22_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9170131.1"
                     /translation="MKKLATLLTVFIGLFIMTGCQSNQNDQASSKASTQATSEVAKSS
                     ALIKLTDSDKEITSKTVTYKSGESLLVILKANFEVKERDGFITSIDGHAQDESQNKYW
                     TFTINGKMGEKGAGETTLDNDDQVVFNLGVFK"
     gene            3030..>3271
                     /locus_tag="IWI22_002817"
     CDS             3030..>3271
                     /locus_tag="IWI22_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9170132.1"
                     /translation="MSNFKGKQFEKDIIMVAVCYYLRYNLSYRDVSEILNERGINVCH
                     TTVYRWVQEYSQVIYCLWKKRNKSVGDSWRMDETYI"
BASE COUNT         1126 a          500 c          696 g          949 t
ORIGIN      
        1 tattattggg aatcccagct taaatcatag ataccgtaag ggattttatt ctttatttaa
       61 aactttacaa cagaaccatt tagttgaaca aggcgggagt cgtcggggag cacaaatgat
      121 catgttgaat gattggcatc cagatatttt tgaattcatt atctctaaga ttcagaatcc
      181 ggccatttta caaaatttga tccacactag ccaaagtccg acagtgcgcc aattagctag
      241 tgaaaaactg acttttgaac cactgacagc aaatgaaaaa gaatggttag aatttttggt
      301 tgatcatccg acaaatgata tggaggataa atttttaaaa acggctcaac agaaattaaa
      361 agcgggtggt acgtacagtg tgaaagaccc tgatttcttg accggagcca atatctccgt
      421 ctgtttaaca aaagaattta tggaagctgt cgaaaccgac caaacttatg agttacgctt
      481 tccagcaatc gaagactatg acgctagtga aatggctgat tatgatcaac attggatgga
      541 agttggagat gtcagagaat gggctgaaac agggcgtaaa gtaaaaatac atcaaacgat
      601 caaggctcgc gaattgtggg atttgattac ttattgtgca acgtattcag cagaaccggg
      661 aatatttttt atcgataatg ctaatgagat gaccaatgct caagcctacg gacaaaaggt
      721 agttgcaacc aatccatgtg gggaacagcc attgacgcct tatgcggtat gcaatctagc
      781 agcaattaat ttagccaata tggttgataa aaaaacgaaa gttgtcgact ttgaaaaatt
      841 aactgatgtc gtaagagcta gtgttcgttt tcaggacaat gtaattgatg cgacgcctta
      901 cttttttaaa aaaaatgagc agcaagctaa aggtgaacga cgtgttggac ttggggtcat
      961 gggattggca gatttattga tttattgtga aaaagtctat ggatctgaag atggaaaccg
     1021 tttagtagat caagtatttg aaaaaattgc tgttacggct tatcaaacat ctgttgaact
     1081 ggcaaaagaa aaaggcagct ttccattttt agttggtaaa acagctgagg aaacaaccgc
     1141 gcttcgtcaa cgttttattg agtcgggcta tatgaagaaa atgccggaat cgatccgaca
     1201 agcgattcta aaatacggca ttcgtaattc tcatttacta acagtcgcgc caacgggatc
     1261 aacgggaact atggccggtg tttcaactgg actagaacca tatttctctt tttcttattt
     1321 tagaagtggt cgtttaggaa aattcattga agttaacgct gaaatagtca gtgaatattt
     1381 agaaaaaaat ccagagaaaa ctgcggataa tctaccacca ttctttgtct ctgccatgga
     1441 gctttcacca aaagctcacg tgagtgttca atgtacgatc caacgttggg tagatagctc
     1501 tatctcaaaa acagtcaatg cacctaaggg ttatcaagtg aaacaagtag ctgcgatcta
     1561 tgaagatctg tatcaaggcg gtgctaaagg tggaactgtc tatgtggatg gtagcaggga
     1621 ttcgcaagtg ttgacacttg aagcagatga caatgtattt gaagaacctg tagtaggggt
     1681 ggcagataat caagttactc ttgactcatt agaatcatca gaagcagaag aaatatgtcc
     1741 gatttgccat gagggtagaa ttgaagaaat tggtggttgc agtacctgca ctaactgtaa
     1801 agtacaacta aaatgtggct tgtaaaagga gctattcgat gaaaatttat actaaaagcg
     1861 gcgataaagg aaatactagt actagtatta ttggtggaga aagaataaaa aaatctgacg
     1921 ctcgcgtttg tgcttacggt agcgttgatg aagtaaattc ttgggtcggt aaaattattt
     1981 cagagttgga tgataaaaag tttgagctgt tgaaaaaaga actagttgta ttacaaattt
     2041 tactttttga tattggaaca gacttggcta cacccatcgg gattaaagaa atgattgtag
     2101 ggaaagaaga agtaactaag attgaaacgc tgattgattt ttatcaagca aaagttccag
     2161 tcattgaaaa atttatctta cctggaggac atccggtggc atgcgatatg caaattacac
     2221 gaacagttat tcgtcgggca gaacgtgaaa tcacacgatt gattgtttca gatgaaccta
     2281 tcaatttaca tgcatacaaa attatgaatc gcttatcaga tttatttttt gtacttgcac
     2341 gttatgtaaa tcatgtttat gctaccaaag agccatttta tgaacgagca gggaaggtat
     2401 tccattaaga gagggtttta aacttgaaaa aattagcaac attattaacc gtttttatcg
     2461 gactattcat tatgacaggg tgccaatcaa atcaaaatga tcaagcctct tcaaaagcat
     2521 ccactcaagc aacaagcgag gttgctaaat caagcgcgct gataaaattg actgatagtg
     2581 ataaagagat cacttcaaaa acagtcactt acaaatcagg agaatcatta ttggtaattt
     2641 taaaggctaa ctttgaagta aaagaaaggg atggctttat tacttcaatt gatggtcatg
     2701 cgcaagatga gagtcaaaat aaatactgga cattcactat caatggaaaa atgggtgaaa
     2761 aaggcgcagg tgaaactaca ttagataatg atgatcaggt agttttcaat ttgggtgtat
     2821 ttaaataaaa attatcgttc tttacagcaa ttgagcgata attaagtaag agaaaattat
     2881 tagtttttaa aggaatatgt taattttaaa acacgttcaa accaatcatt tcgaaggggt
     2941 tgaacgtgtt ttttggttct gttgcaaagt tttatctaaa tacctattta gtgtaaaata
     3001 gctgaaacta aaacataagg agaattacca tgtccaattt taaaggtaaa caatttgaaa
     3061 aggatattat catggtagcc gtatgctact atttaagata taacttaagt tatcgtgatg
     3121 tttcagaaat ccttaacgaa cgtggaatca atgtttgtca cacgacggtt tatcgatggg
     3181 tacaagaata tagtcaagtt atttattgct tatggaagaa aagaaataaa tctgttggcg
     3241 attcctggag aatggatgaa acttatatta a
//
LOCUS       DACDNO010000136         3007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDNO010000136 DACDNO010000000
VERSION     DACDNO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.143"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(276..692)
                     /locus_tag="IWI22_002818"
     CDS             complement(276..692)
                     /locus_tag="IWI22_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170133.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="IWI22_002819"
     CDS             complement(695..979)
                     /locus_tag="IWI22_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170134.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="IWI22_002820"
     CDS             complement(1679..1774)
                     /locus_tag="IWI22_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9170135.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="IWI22_002821"
     CDS             2427..>3007
                     /locus_tag="IWI22_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9170136.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALREL"
BASE COUNT          957 a          478 c          565 g         1007 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caacgtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggcttttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     3001 gagaact
//
LOCUS       DACDNO010000137         2986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDNO010000137 DACDNO010000000
VERSION     DACDNO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.144"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(176..1132)
                     /locus_tag="IWI22_002822"
                     /pseudo
     CDS             complement(176..1132)
                     /locus_tag="IWI22_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(1177..2303)
                     /locus_tag="IWI22_002823"
     CDS             complement(join(1177..2075,2075..2303))
                     /locus_tag="IWI22_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9170137.1"
                     /translation="MTRYEENFKQMIIELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIILKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(2494..2826)
                     /locus_tag="IWI22_002824"
     CDS             complement(2494..2826)
                     /locus_tag="IWI22_002824"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9170138.1"
                     /translation="MIAVSEATYKAFKTEFVSQYQFETMAHLELECFDYVNWWNHHRI
                     HSSLGYKTPIEVLCGGWRSEPLIMSKNRLPSVRAAYFIQSEHAAKKTDLRRLVVKGNW
                     IWCHKQTL"
BASE COUNT          882 a          578 c          482 g         1044 t
ORIGIN      
        1 cgaataaacg gtattcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca
       61 taagatgttg tttttacttt gctattctta ttacttgatg caggacagct ttttcacaac
      121 ctcttttatt tcaaataacc agtaaataca atgaacatct acaaaaaact tctatttatt
      181 tatagatttc gtcaggcaaa tccaattctc tcttatcaat caaagtctga taccacgaag
      241 cagatttttt caatgttctg cttcgttcat cgttcaattc gattcgaact aatccatatc
      301 gattcttaaa tgcattcatt ggtgacacac agtcagtgaa tgcccataac atatagcctt
      361 cgcaatttgc gccttcttct gttgctttga tcaaataacg caaatgctgt ccgataaatt
      421 cgatacggta atcatcttgc acgattcctt tctcatccat ataccgttct tcatgttctc
      481 ttcccatacc gttttctgtt atcagccaag ggatattatg ataattttct tttagataca
      541 tggctatatc atacatgatt ttcggataga tttcccatcc cctggaatta ttcattttct
      601 tcccaggtaa atcaaagact tcataatatc gttctggatg gaaaggtgtc tcttcattcc
      661 attgataacg aggagcgcga acgcgtttag gataatattg attgattcca aggaaatcta
      721 ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg atcgaaataa atcgattgtt
      781 tttgacacaa ctccaggaac tcttctgtgt agtacccttt taccatcgga tcaaaaaata
      841 ctcgattata aaacaaatca tacatataag cagctttttg gtcttctgga gaattagatc
      901 gagcatagac catttcagga tttaaaatac agccaatcct tccaccataa tttccgctgc
      961 ggtaaatctc tactgcttta gccgttgcta aaactttatg ataattccac atcatccact
     1021 tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa atagcaacgc gtttggggaa
     1081 cgattggttc gttgaatgta aaccagtttt ttaccctatc accgtaacga tcaatggatt
     1141 tgagtcaata tcttggacac attttagtag agacggttaa gccatttttt cttttgctaa
     1201 ttcttcaaat tcctgtgggg ttagatagtg aatcgagcta tggattcgat ttcggttata
     1261 aaatccttca atgtagctaa atagcgctcg atttgcttct tcaaaatctg agtagctagt
     1321 cgtgtagact tcttcttttt tcaatgaggc atggaaagat tcgattccag cattatcata
     1381 aggtgttcct tttctactat aagaatgcct tattttgttg gttttaagcc attgttccac
     1441 ttctcttgct gtgtattggc tgcccaagtc agtgtgtaga atcattcctt ctggaatgtg
     1501 ataatgtatt tttgctttat tcaatgtttg aatgacacaa tccacagtca ttcgttttga
     1561 aaatgtataa ctaataattt ttttcgtgtg taaatccatg attgaagata agtaacacca
     1621 accatttttc ttagtaggaa tgtacgtaat gtcagctgcc catttctcac agatcgtttc
     1681 agtagaaaag tcctgattta agagattctc ttttaaaatg atcggtttat tagacctttg
     1741 aggtctatat tttttaacta caatcgacct taaatttaat tgtttcatta gcttttggac
     1801 atgtttaaga gatactgaaa tcccttcttt taataaaact tgatggatct ttgtggcacc
     1861 atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc tttttcaact tattatttct
     1921 gatttctgta gctgtcaacg gtttattctt ataaaaataa tagacacttc tagggatttc
     1981 taataaccga cacaacaaag aaacagtgta atccttacac catttttcaa taaattgaac
     2041 aagcgaattt aggttttccg agagaatatg gctgcggctt tttttaagat ttcaagttcc
     2101 tcatttaaac gagcattttc ttttctcagt tcagctactt cttttccagt cagtcctgtg
     2161 gactgatcag gtaaatataa attcttccat ttgtaaatcg ttgcttcaga taagccatat
     2221 tctttcgcta accctcgaac agaacgtcca gtttgattca gttcaataat catttgttta
     2281 aaattttctt catatcgggt cataaaaatt catcctctcg tagataagtt taatttattc
     2341 ttgtctctac gaaaagctgt ctaagatttg atactaacac caattggata gatgtcaata
     2401 aagtagacac aaaaagagaa gatacagact gtagacaaag tccattttat ggatgatgtc
     2461 tacatggaat ggcaacactt ttctagacaa aaattataag gtctgtttat gacaccatat
     2521 ccagtttccc ttgacaacga gcctcctcaa atcggttttc ttagcggcgt gctcactttg
     2581 aataaaataa gccgctcgaa cagaaggtaa ccgattcttg ctcattatca agggttcgct
     2641 acgccaacct ccgcatagta cctcaattgg tgttttataa ccaagagaac tatggatccg
     2701 atgatgattc caccaattga cataatcaaa acattctaat tctaagtgtg ccatcgtttc
     2761 aaattgatat tgactcacaa attccgtctt aaaagctttg taagtcgctt ctgaaacggc
     2821 aatcatatgg acatccaggc ttactcaagg agcgttgaat gccaaatgtt tctaagataa
     2881 gatcaatcaa ttcattatca aattcttttc cacgatccgt atggaagagt tggaatggca
     2941 acagtttttt tgacaaattt tataaggtgc agaacttctt tccgta
//
LOCUS       DACDNO010000138         2931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDNO010000138 DACDNO010000000
VERSION     DACDNO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.145"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            181..831
                     /locus_tag="IWI22_002825"
     CDS             181..831
                     /locus_tag="IWI22_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9170139.1"
                     /translation="MSLITLKNIKKVYGKNQILFENLSLEIEKGEFVGLVGKSGAGKT
                     TLLNIIGLITDISEGIITIDGQQKLSVNSRNAMLLRRYTIGYLFQNYGLIEDESVLWN
                     LKLALEYKKMKKEDKLKVINEYLKQFNLSDMLNKKIYQLSGGEQQRIAIIKLILQGSQ
                     IILADEPTSGLDKENEQVVMKLLKELNEKGITIIMVTHNMDLRNYFSRVINVETFR"
     gene            complement(1291..1905)
                     /locus_tag="IWI22_002826"
                     /pseudo
     CDS             complement(1291..1905)
                     /locus_tag="IWI22_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2032..2418
                     /locus_tag="IWI22_002827"
                     /pseudo
     CDS             2032..2418
                     /locus_tag="IWI22_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2706..>2931)
                     /locus_tag="IWI22_002828"
     CDS             complement(2706..>2931)
                     /locus_tag="IWI22_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP9170140.1"
                     /translation="FFSQLAKIVTIAGTTGFGLYDLKILWFVIPAAIVGGLLGAKVST
                     VLSPQKVTLVFQTVIFLVLIINLYNGYQLL"
BASE COUNT         1011 a          464 c          533 g          923 t
ORIGIN      
        1 attaaaaaat cgttatatat tttggggaat agagttgatt gcatcatgta tattgactgc
       61 tataaattac aattttgctt gtctttttgc aattatactt attgactata tattctgtga
      121 attactttat agaatttata tcttaaacgc tttgtatgaa atagaaaagg gggcataagc
      181 atgtcattga ttacattaaa gaacataaaa aaagtatatg gtaaaaatca aattttattc
      241 gagaatttgt cgttagaaat agaaaagggt gaatttgttg gtcttgtagg taaaagtggt
      301 gcaggtaaga caactctttt aaatataatt ggattgatta cagacatatc agagggaata
      361 attactattg acggtcaaca aaaattatca gtaaacagta gaaatgcaat gcttttaaga
      421 cgatatacga taggctatct atttcaaaac tatggactta tagaagatga aagcgtatta
      481 tggaatttaa aattagctct tgaatataaa aaaatgaaaa aggaagataa actgaaagta
      541 atcaacgaat atttaaaaca atttaatttg agcgatatgc tcaataaaaa aatatatcag
      601 ttaagcggtg gagagcaaca gcggattgca attatcaaat taattttaca aggaagtcaa
      661 attatactag ccgatgaacc aacttctgga cttgataaag aaaatgaaca ggtagtgatg
      721 aaacttctga aagaattgaa tgaaaagggc attactataa ttatggtaac tcataacatg
      781 gatttgcgta attatttttc cagagttata aatgtagaaa cttttagata gtttattaaa
      841 gggagatata cttttttgtg caatctccct tgttcttttt ctgtcatatc aagactcgtt
      901 caataatggt aaattcgtgg taaaatagct acttttttag ttgatgaata gcccttaaat
      961 actgtattta tgcggataat cggatttttt ataccaagtt tggatatatc aacaaatcat
     1021 ttgataatta tgatcttggt tttataatta atttaaaaaa aataaaaact atcgatgact
     1081 tactcacaaa taatttagtt ctcaaaaaag cgtactgtga tacattctaa tgacgagagc
     1141 gtctttaccg gaaaaatcaa tcttttagat tcaaaaaaac tagtactttc tataatagat
     1201 tacgaaagca tcacaaagac gaacaaaaaa aacaataaat atagatgcag actgtagaca
     1261 tcatccataa aatggacttt gtctacagtc tgaaacgaga tctttcttct tacgtgaagg
     1321 aagaatatcc agtaatttcc cagtatcgcc atccatacaa gaaaaagaat agggaccgac
     1381 ttgatttttc aatgaacgaa attcatccac caatgaacag cgaggcaacc aattcctttt
     1441 cacgtttgtc atggggagca gagaagcgag tgttcgcgaa acggttgacc aagaaacgcc
     1501 gcaatgttta gaaatcgtcg tcattgcttg tttttcagtc aattcctcca atattttata
     1561 atgtacttgt ttggcgataa aacagttttt ttcaacaatg taggtattcg ccgtaaaata
     1621 ggtgtgacag ccacgacaga ggtaccgttg ctttgccaaa gcaagataag tccgttgatt
     1681 cgcacactta ttgagaagga ccagcgattt tttctttccg ctcttaacga tcagcccatg
     1741 ttgcttcacc tgtttacact gaggacagcg ctgaatcgtt ttaatgagcg tggcagaaac
     1801 aacgatactg ttgattgaat gtattttttt cttctcgaca tgagtgattt gaatgttttt
     1861 atctttgatt cctagtaata gtttgataag atgaagatca ggcatacaga acgctccttt
     1921 gattgtgttg tggtgattca attctaacgg agcttctgta tgttttgttt ttttatgctc
     1981 aaaaggactg atagaatgat tttctatcag tcctagaaat tatagcgccg gaaaagtttc
     2041 cgagcaccaa aatacccatt tctcactgaa gcaatgatga ttgatcgatt gcttgagttt
     2101 tctgcgcctc taaaggaggc gtatcccttt tttcatgaat tagtcgaagt ctttcgagac
     2161 aaagaccctg acttattttt ctccttattg gcagaacttc ccgaaacgtt agatgacggc
     2221 tttcgggaga agcttcaaaa ccttctgacc tatgaagaag gtatcaccaa cgcaatgatc
     2281 tatccttatt ccaatggaaa aattgaagcg aagaataccc acataaagac aatgaaacga
     2341 gtatcctacg gatttaaatc attcgagaat atgagaatta gaatcttttt gatcaatcaa
     2401 ttaatcaatg taagataaca aaaaatctga gccagaatgt ggttcattct gacccagatt
     2461 tgattttgca caactcatca gtccttattg acaaagagcc tattttgtta gtagatgtct
     2521 ggaaacggta ctttgcacat ggtgaacagg cagtgctatt agaaaaatca tgcatattac
     2581 taatgcaatt gagagtattt attctagttt cagaaaagtc acaaaaaaga gcgcttctta
     2641 atgagaaagt acttctaaag ttactaatta atcgttttct ataagtggtt gataatactc
     2701 gttttttata gtaattgata gccgttataa agattgatga ttagcaccaa gaagatgact
     2761 gtctggaaga ccagcgtcac tttttgtggt gagaggacag tgctgacttt tgcccctaac
     2821 aaaccgccaa caatcgcggc tggaatcaca aaccacaaaa tctttaagtc atagaggcca
     2881 aatccagtgg tcccagcaat cgtcacgatt ttcgccaatt gagagaaaaa a
//
LOCUS       DACDNO010000139         2918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDNO010000139 DACDNO010000000
VERSION     DACDNO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.146"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..94)
                     /locus_tag="IWI22_002829"
     CDS             complement(<1..94)
                     /locus_tag="IWI22_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9170141.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREV"
     gene            complement(545..862)
                     /locus_tag="IWI22_002830"
     CDS             complement(545..862)
                     /locus_tag="IWI22_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9170142.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTIHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQIK
                     LGF"
     gene            complement(863..1117)
                     /locus_tag="IWI22_002831"
     CDS             complement(863..1117)
                     /locus_tag="IWI22_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9170143.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1377..1595)
                     /locus_tag="IWI22_002832"
                     /pseudo
     CDS             complement(1377..1595)
                     /locus_tag="IWI22_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          956 a          506 c          409 g         1047 t
ORIGIN      
        1 gaacttcacg atagctaagg ttataacgaa gatagtagcc cacggctaca ataatcacat
       61 cctgctgaaa ttgctttcct ttaaaatgat tcatcgtcat tcctcctgct atctttttct
      121 attattctac cttatttgat agtagattta aaactttgca acagaacctt taaaacacaa
      181 aaataaatac taccttattt aggtagtatt tattaaaagt tacgtcaatt ttgattaaaa
      241 atatactact tgaacctctc atgagtaaag tcacgaaatt tttgggaata gagcgtactt
      301 gatatcttat tttttcacta atagcagtgt ctcttattca ccaagcgatc cccgtagttc
      361 ctacggttct taatttttct ctagaccaat gattgtatct ttagaccttc ggccataaca
      421 tggagcaaaa taaaaaggta ctaagtctta tatatcaatc ttcgagtaac cgacggttaa
      481 caaccatatc gagcggcatt aatcacccac ctgtagagga tgagcgactt ttgcctaatt
      541 attgttaaaa gcctagtttt atttgtttag attggaaaat acgtcctaca agtatctctt
      601 ttttttcatg gtcgatccga taaaataagg catagctttt gccgatcacg atccgatatg
      661 ttgggatatc cattccatag atttcggaaa cttcctcatg tatagttgga aaatttttgg
      721 ttagttctaa agccttctgg atcgcaccta caaatctttt tatgctggct ggagaaagag
      781 caagctcatt ttcccattct tctattatcg tttcaaggct ggtctggaaa cttttagaat
      841 aaactagcgt gtactctttc atctattcac tccagccgta ttttccgaaa gcttttacca
      901 tttcaatttc tgaaactgtt aaacctttct caaagtcttg gaaactttct tcaatcaatt
      961 tgcgatcgtg ttcttttgct atttgagtag tatctaatat gaataagttg ccttcttgtt
     1021 tggcttggat agtgtctccg ctttttaatc caagagagct tggaaggatc actcccacgc
     1081 tattgccgat ctttctaacc tttagttctt ttcccatgtt atcacactcc ttttagtttt
     1141 tgatgaataa attataacat gttataacaa caattgccaa cggtgtttct taagggattc
     1201 cactccatat tattattgga caaaataagc cacttagaac gctgaaaaca tatacaaacg
     1261 atatcaaaat acgagtgaac tactcaccat acacttccgt taacatccat ttaagtaggt
     1321 atttttatta tatatagatt taacatatgt cggtcggttg attgatatag ctaaagttat
     1381 aactcaaccg aagccgattc atctacacga ataaagttgc gagtgttctc ggctaattca
     1441 taaaaattgt tatgtgttcc atatagcaat actggaataa ctttattttc ttgaatctca
     1501 taagcaactc tatacacggt gaccgcatac ttaaatccat tagaccaatt acctgataaa
     1561 cctccagttt tttgctctta cttaaatgga tcaatagcaa tttcatcaca aacctataga
     1621 ggatgagtga cttctgccta attacgttaa aaaatattga aagtaactct atgtagcaaa
     1681 aaatatctag aatttcattt cctcaaaact gatcgatatt gtatctaaaa atatatacta
     1741 cctaaaatag gtagtatata ttaaaatatg tgcttttgtt agaaaaatca atacataaat
     1801 tatcatatct gtatatgtca tttaggtatt tcagttatgt tataatagtt ttgctgtctg
     1861 ctaccccggc ctaatctggc aaggggagga ggagatataa tgaaatacct ttttgatttt
     1921 gtttttgcaa tcaagccact tgcagatgta gtgtgcagtt acctctgcaa atggcttgat
     1981 tgcaaattgc caaaaaagac agtaagccta cccgttaatt ctataatata gaaacgtaaa
     2041 aaaaacccca tcatcctgca cgctgatggg gtttggtgat ataatgaaat acctctttgt
     2101 tactcataat tataccataa ttagtcacga atgcaaccaa tagaaaaaac tacttagaac
     2161 ggtagatcat cgctaataac aatgcattgt ccaccaaatg gattttgtga agcattaaat
     2221 acattgttat taattgtcgc tgcttttaac taggcctctc cccttctatt ataaaagaag
     2281 atatatatca tttcttaatt acctactacc ttaaaccaaa atttaagtac tacctaattt
     2341 aagtagtact taaacaatct aaaaaatagt tatcccaatt gttatgcttt tataatacga
     2401 ttatttgtaa caaaatgaat attatataat taaacactac catatttagg tagtgtttaa
     2461 ttattagcaa aacaaaaaat atttattcta ttctcttaac ttcaactaag ttatgctgca
     2521 cacctttata taaatcatat gcaactcatt agctagacaa actacaagca tgtagtttta
     2581 tctgttaatt atgtactacc taaataaagt agtttataat taacaagtat atatatttaa
     2641 aaatttaaga ttaatttttt tatgacttgt acaaatttat ctgatattct cttattaaga
     2701 actacctaat ttgggtagtt cttatttttt acattttaag tagtatttaa ttgcaatttg
     2761 gtagtatttt tttattatct tattcttaga acggtagtac ttaatataaa tataggtagt
     2821 atctaattta agtattaatg gtagtatcta aattaacagg tagtatgtaa ctactttaaa
     2881 gtattttttt tcagattaac acagtagtat cttattaa
//
LOCUS       DACDNO010000140         2918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDNO010000140 DACDNO010000000
VERSION     DACDNO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.147"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1361..1879)
                     /locus_tag="IWI22_002833"
     CDS             complement(1361..1879)
                     /locus_tag="IWI22_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9170144.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          968 a          528 c          413 g         1009 t
ORIGIN      
        1 ttttacgttc tattcagatt aacgggtagg cttactgtct tttttggcaa taattttgca
       61 atcaagccat ttacagatgt aatgcgcgga tacttctgct aagattgcaa aaaacatatc
      121 gaaaaggtaa ttcataatac aacctcctcc ccttgccata ataggccggg gtagcagaca
      181 gcataaatat tataccataa ctgaaaattt cattttagta catttcaata tattgatgaa
      241 ccagcacaat aaaacatgcc atgactaaca gaaaaaaact tattaaacca gatataactt
      301 gtaaacatcc aactatatag aaaacgtaaa ttttctatat agttggatta agataacgac
      361 tatttatttt atcatatttt tactataccc tatactttta cttggtatat ttatgaataa
      421 tcgcaaattt acccctatac tactagtttg taagttagat taataaaaat actaaagtta
      481 tttgttcttt tattaaatac acctacaact tataaattga tggtatttag ataacacgaa
      541 agaataaaaa taacagctga tagtaaagtt tgttgacgat tgctactcaa tcgaaagtga
      601 gttagtgcgt atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt tacatcttgc
      661 agaaatcggt actgggatta aactaagcaa taaaaactaa tgttcccctc attctttgaa
      721 cttctactat ttgatttatt tattctgata aatgacgatt tcaccttcag gatcttcaaa
      781 tcccgtatag aatttattta agatctcttc agtatcagta ataggatagg cgataggcca
      841 catacccttt gatggttaga ataaactgct caaaatctgt atcgtatgtt ggctttagca
      901 tattaacaaa ggctatctcc aaattataaa ccataagcgt tgaatcgttc ttaacaattt
      961 caattcttcc taacccaaag tcagaagata taattaccgg acttgcctta cccttcatgt
     1021 gggttgttga gattccatat ttaaagggaa ttgtcggtac tatcggcggt ctgaaaaaca
     1081 tcccaaaaat aaaaaaatct ttcccctgct agtctcctaa gtccctttgt agcggatatg
     1141 gcaccataat ctcattttat cgaagtgagt catggtttat ctgttggcca accaaaattg
     1201 atacaatcta acgatgcacc ctctacggca ttctagggga gtgcacttgc attcacccaa
     1261 taaaaaccgg cagttagcaa agaacttatg tagtcgtatg ttcttactgt taacatacaa
     1321 gaggtctcct ttccacaatt gcttagcagt aaacgggaat ttacaaagct aatctcttcc
     1381 attttcttat ctttgttcta aaagtcccaa atggagcaac tgtatttaca tgaataaatt
     1441 tatatacttc ccatacagcc gttttggttg cctcgtctgc ccattttctt ttgtgtgcag
     1501 tgaatagatc ttcatcactc attgaatcaa tcatcatgtt aatatcatca atattacgat
     1561 tgagaatttc ctctaattct ttcaatggta gatgtgcata ggtatttgta aaccacttat
     1621 ataactctcc taactgattc catttaaatt gatcagaagg cgttttaaca gataatccat
     1681 ttctttcatc attttcccat tttagaatta aattcgtcca accaacttga tatgctaaat
     1741 tttcagcagg tgttctatcc acttcctcaa ctcttttatc cttcaattct tcaggaatag
     1801 tttcaaattc taaaatatat ttagtataag tcctatttat ttcttttttt aattcttctt
     1861 tatttttata tgttctcatt atattacctc cataaattcc taattgtctc ctttaattca
     1921 tctataattt catccacgat attttaatcc gttctacaat gcatatctga tatgcattga
     1981 ctgtctaact catatgtgaa tgatttataa aatatcccaa cgtctcatct aaagaaatgt
     2041 tgatcgtcag caaggagcta aaaaatacga atatatttct tttgtagtaa aatcggactg
     2101 gtttgcatat cattttcttc gtaaatatca aaatcatgtt aactactatc aattactaat
     2161 aaaaactacc actttaagcg ttaagaactg ttaagtttag ttaagtgagg atagttcaca
     2221 aaatccattg ttaaacttca cgaaagtggt aaaactcaaa atgctttata taggattaca
     2281 gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa aaattgacga
     2341 tgacacgttc cttacggctc atcagatgaa acagctacaa aaacgaaatg atcaactcga
     2401 agaaaattta attctaaaaa agcgattgcc atattcacgc ctcgctcgac gaactattaa
     2461 aggtcaagat ttacctgaag atcatccttt ttaggaaatt gaagcttttt atgttgcaca
     2521 atgcatttat aatactacaa tgatgttttc accagatatt cttctagggt gcggtgtaat
     2581 gaaacagata cacttgcaaa agaaaattca ccgcgaattt actaaaatta tgaataatta
     2641 tgttaatctt ccgaaatttt tttccaaaat taagggatca agctggtgtt atagattgtt
     2701 taaggttggc aaaaaaataa cttcttttta aacaattata gaaaaccgat actctgttaa
     2761 gttggttgac acggtgtcga tttttatgac tttcttattc tgaaacttca ctaacggaca
     2821 gtacctaaaa agtctatagt atggacgttt acgagtacta ttttatcaaa aatgctgctc
     2881 atttcaactt atatgtttta gtgaactacc cccactta
//
LOCUS       DACDNO010000141         2857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDNO010000141 DACDNO010000000
VERSION     DACDNO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.148"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            562..843
                     /locus_tag="IWI22_002834"
     CDS             562..843
                     /locus_tag="IWI22_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170145.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="IWI22_002835"
     CDS             845..1261
                     /locus_tag="IWI22_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170146.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="IWI22_002836"
     CDS             complement(1422..1688)
                     /locus_tag="IWI22_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170147.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="IWI22_002837"
     CDS             complement(1646..2620)
                     /locus_tag="IWI22_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9170148.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DACDNO010000142         2780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDNO010000142 DACDNO010000000
VERSION     DACDNO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.149"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1014..1304)
                     /locus_tag="IWI22_002838"
     CDS             complement(1014..1304)
                     /locus_tag="IWI22_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170149.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="IWI22_002839"
     CDS             complement(1512..2204)
                     /locus_tag="IWI22_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9170150.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            2599..>2780
                     /locus_tag="IWI22_002840"
     CDS             2599..>2780
                     /locus_tag="IWI22_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9170151.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT          824 a          518 c          397 g         1041 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacatatgt aattttaata taaatactat caaatactca tttaatcctc cttttttatc
     2341 tatttaaaaa atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt
     2401 tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga
     2461 ttactgtatt tattacaaaa aacgcggatt tgtcataaat tgtcatgctt cttttaccta
     2521 tgaaatattt tcacgtgtta aagtattcct cgttgaacac aacaaataaa actttttaac
     2581 gaataaagga gatttttcat gacatcactt aaaactttac tttttggaac aactttggct
     2641 gccggcgctg cattcttcat gggaacgact gctcatgcgg acgaagctta taccgttcaa
     2701 tcaggtgata ctctatcaac gatttctcaa aaatacgttg gtgataactc cttgatcaat
     2761 gccattgcag aatcaaattc
//
LOCUS       DACDNO010000143         2732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDNO010000143 DACDNO010000000
VERSION     DACDNO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.150"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..335
                     /locus_tag="IWI22_002841"
     CDS             <1..335
                     /locus_tag="IWI22_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9170152.1"
                     /translation="KLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKK
                     TGGLRYAPALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKRED
                     FNEAQKIR"
     gene            351..1112
                     /locus_tag="IWI22_002842"
     CDS             351..1112
                     /locus_tag="IWI22_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9170153.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            1243..2577
                     /locus_tag="IWI22_002843"
     CDS             1243..2577
                     /locus_tag="IWI22_002843"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9170154.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT          926 a          464 c          585 g          757 t
ORIGIN      
        1 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
       61 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
      121 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
      181 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
      241 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
      301 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
      361 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
      421 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
      481 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
      541 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
      601 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
      661 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
      721 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
      781 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
      841 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
      901 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
      961 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
     1021 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
     1081 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
     1141 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
     1201 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
     1261 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
     1321 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
     1381 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
     1441 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
     1501 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
     1561 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
     1621 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
     1681 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
     1741 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
     1801 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
     1861 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
     1921 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
     1981 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
     2041 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
     2101 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
     2161 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
     2221 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
     2281 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
     2341 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
     2401 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
     2461 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
     2521 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
     2581 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
     2641 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
     2701 caatcaaatc aaaaacatga gaagctgttt tt
//
LOCUS       DACDNO010000144         2703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDNO010000144 DACDNO010000000
VERSION     DACDNO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.151"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWI22_002844"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWI22_002844"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9170155.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1058 a          419 c          555 g          671 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tga
//
LOCUS       DACDNO010000145         2634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDNO010000145 DACDNO010000000
VERSION     DACDNO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.152"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWI22_002845"
     CDS             5..1150
                     /locus_tag="IWI22_002845"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9170156.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWI22_002846"
     CDS             1321..1779
                     /locus_tag="IWI22_002846"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9170157.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IWI22_002847"
     CDS             1938..2408
                     /locus_tag="IWI22_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9170158.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IWI22_002848"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IWI22_002848"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          862 a          505 c          496 g          771 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc accccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatttgaa gtca
//
LOCUS       DACDNO010000146         2586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDNO010000146 DACDNO010000000
VERSION     DACDNO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.153"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(80..379)
                     /locus_tag="IWI22_002849"
     CDS             complement(80..379)
                     /locus_tag="IWI22_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170159.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            complement(420..1037)
                     /locus_tag="IWI22_002850"
     CDS             complement(420..1037)
                     /locus_tag="IWI22_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9170160.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1385..2557
                     /locus_tag="IWI22_002851"
     CDS             1385..2557
                     /locus_tag="IWI22_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP9170161.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          865 a          503 c          428 g          790 t
ORIGIN      
        1 aactagttat ttcaatgccc caacgatcat aagattttcg cttcataaaa attttcctcc
       61 ttgtaaattt tactaacgat tataaatacc gttgccattc tttcaactgt tgactaatta
      121 acgcataaaa ttcgacttct gataaaatgg taatctcatg acctaacgca atcaagtccc
      181 gcgcggtttg ttcctttttt gtatagagag aatcatcaaa caacgactta gaatactgac
      241 cacaaaccag gaaatttgtt tttgaagtaa cacttgattg acaatggccg tttaaagagt
      301 gaaccaaacc gatggcttgg gttcgtgtca tttgctgcag cgttccagta aaaacgaccc
      361 tttgattcgt taagtccatt ttcaaactct cccttcccgt ctaatagctg ttatatctat
      421 caagaaaagt ggtgcaaggt atgttcatat tctttcaccc gtcgataaaa ggtatttcgt
      481 ttcatgccca attgatccat agcatagact gcagttatct ttcctgcttt ccagtcagaa
      541 tagatcgcag gaaattcagg aggtaaagca attttctttc tgccgaattt tacacccttt
      601 tgtttcgcag cttctacgcc ttctctttgc cggctattaa ttcgtttccg ctcgtcttcg
      661 gccatatagc tgagtaattg cagaattaag tcagagataa gcgtctctaa cccatctagt
      721 tgcttgtaac tagcagtatc taacagaggc atgtctaaca cagaaatatc aatctgcttt
      781 tccttagtta attctcgcca ttcttctaaa atcatttgtt tatttctgcc tagtctatct
      841 aatgattgta gatagagtaa atctcctttt cgaaacatcc gtttcattaa ttgatattca
      901 gggcgtttaa aatctttgcc gctggctttt tctacaaata tatattcttc agggattcct
      961 aattccttcg cggaaatcag ttgtcgttcg atcgattgat cataactact gacccggata
     1021 taaaaaaatt ttctcatcgt ttttatctac ccttttttca ctaaaattgt ttcaaaacaa
     1081 tcagattaaa tcagaaacgt ttcaaattgt attatgtggt tattatgcca tacataaaca
     1141 aagaaaatca aggcatgtca aaaagtatac tttttgaaac agtaagttta cactcccgca
     1201 tgattatatt tattccgcta aaatacataa aaatggcagg ccttaacggt ctgccattaa
     1261 ataaactagt tatccagttt ttcgataaag tccgtataat tgtgtaaaag taaaaaggcc
     1321 atataacagt ccttttacgg tacaatgttt ttaacgacaa aaacataccc aggaggactt
     1381 ttacatgacc caagtacatt ttacactgaa aagcgaagag attcaaagca ttattgaata
     1441 ttctgtaaag gatgacgttt ctaaaaatat tttaacaacg gtatttaatc aactaatgga
     1501 aaatcaacga acagaatata ttcaagcaaa agaatatgaa cgaacagaaa accgacaaag
     1561 tcaacgaaat ggctattatg agcgcagctt tacgacacgt gtaggcacgc tagaattaaa
     1621 agtacccaga acacgtgatg gccatttttc acccacagtg tttgaacgtt atcaacgaaa
     1681 cgaaaaagcc ctcatggctt caatgttgga aatgtatgta tcaggcgttt caactcgtaa
     1741 agtatcaaaa attgtggaag aactttgtgg taaatccgtc tctaagtcct tcgtttctag
     1801 cttaacagaa cagctagaac ctatggttaa cgagtggcag aatcgtttat tatcagaaaa
     1861 aaattatcct tacttaatga ccgatgtact ctatataaaa gtacgagaag aaaatcgagt
     1921 actctcaaaa agctgtcata tagcgattgg aataaccaaa gatggcgacc gtgaaattat
     1981 cggcttcatg attcaaagtg gcgaaagcga agagacctgg acaacatttt ttgaatacct
     2041 aaaagaacgc ggtttacaag gtacggaact cgttatttct gatgcgcaca aaggattagt
     2101 ctctgccatt agaaaatcct tcaccaacgt aagttggcaa agatgccaag ttcacttcct
     2161 aagaaatatc tttaccacca ttcctaaaaa aaattcaaaa tctttcagag aagctgttaa
     2221 aggaattttt aagttcacag atattaactt agcgcgtgag gctaaaaatc gattgattca
     2281 tgattatatc gatcaaccaa aatattcaaa agcttgcgca tcattggatg atggattcga
     2341 agacgccttt caatataccg tacaaggaaa ttcccacaat cgactaaaga gtaccaatct
     2401 aattgaacga ctgaatcaag aagtacgcag aagagaaaag attattcgca tcttccccaa
     2461 tcaaacatca gccaatcgct taattggagc cgttcttatg gacctacatg atgaatggat
     2521 ttattcttca agaaaataca tcaattttga taagtagaaa tggtaaaaac attgtatagc
     2581 atttta
//
LOCUS       DACDNO010000147         2558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDNO010000147 DACDNO010000000
VERSION     DACDNO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.154"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..336
                     /locus_tag="IWI22_002852"
                     /pseudo
     CDS             1..336
                     /locus_tag="IWI22_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            370..2073
                     /locus_tag="IWI22_002853"
     CDS             370..2073
                     /locus_tag="IWI22_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9170162.1"
                     /translation="MKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDN
                     QYAAEAGYYKFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQT
                     VKNEPISKVQNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKE
                     LLNFMSRFPERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVK
                     GFYEKGRGFVRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIF
                     PNRVFDFQLDQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFT
                     GEEEIVMNTRNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMD
                     TSEKAIPYIATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPE
                     IQPAEVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQ
                     HNQVILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKER
                     V"
     gene            2134..>2558
                     /locus_tag="IWI22_002854"
     CDS             2134..>2558
                     /locus_tag="IWI22_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170163.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYA"
BASE COUNT          993 a          435 c          549 g          581 t
ORIGIN      
        1 gacatgacga tcgaaaatat tccaagaatc aaagaagaaa tcgaacgctt tgaaaaaggt
       61 gaatcgactt tttcagctgc cacgatccga aaataccaac gaaaattgga aacattagaa
      121 cagctgaaag aaaaatcaga gaaaggaaaa gaaaatcttc ttcctgaagt acaagcgatc
      181 attgacagcg gacgagtgac gcaatggaaa aagaatccaa ctatctattt tctgaaaggc
      241 tatcgaaaag tcgctttaga actcaatgaa aacgggggat ttgaggaatc taaaaaatac
      301 caagccacgt cggaagaaga aaaggcagct gttcgtgaat tacttgaaga agctacacct
      361 actgattctg tgaaggagaa agaaaccgat catttctatc gagtagaatt caacgaaaat
      421 gatccagcaa aagggttgaa aagttatggt ggggagatcg taacactaga attgatcgat
      481 caactcaaaa cactggataa ccaatacgcc gcagaagcag gctattataa attctatttt
      541 gaagaagtga agaatggaga agtagtcgat cagatacgaa tggatctagg ggatggcgtc
      601 aatagtaatc accccatcta tttagagtta gctagattgt gcgaacaatc acctgaaaag
      661 gaacaagaac aaacagtcaa aaatgaacct atttcaaaag tacaaaacga gattaaagag
      721 acaaaaacaa aacaaaaggg ttatcaaaaa cgaacggcag aagagattaa gcaagaagtc
      781 aaatcacttt cccaacaggc gcttgatgcg gtgaaaaagc atactcattc acctgaagac
      841 gtcaaagaac ttttgaattt tatgtctcgt tttccagaac gatcctttag aaatcaatta
      901 ttgatcgagg aacaatttcc aggcgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
      961 gaaggaatct atatcaaaaa aggagaacaa gggaacaaga tctttgtcag aaaaacggtc
     1021 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa cagaggctac accagaagat
     1081 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aaccttacta cacgattgaa
     1141 aaagtgtttg atatcacaca aacacaattg aaacctgaag actatccgaa aatcttcccg
     1201 aaccgcgtct ttgattttca attggatcaa acagggcaga cagaacttca agttgggatt
     1261 caagcggtag ccaaaaatat tggaattcaa attcgagata tgacagaaag tgaagtctat
     1321 caaagagaat taggacaagc acgtggggcg tacgttcgta acgaatttac tggcgaagaa
     1381 gaaatcgtca tgaatactcg aaatactcct acgcagcatt tagccacaag tattcacgaa
     1441 ttagcccatg ctagaatgca caaattttca gaattagata ctgcaacaaa agaactacag
     1501 gcagaaatga cctcctatat tgtgtgcaag cattttggga tggacacctc ggagaaagct
     1561 attccttata ttgcgacatg gacgaagaat agtcagacgt tggatgataa ggaagctgcg
     1621 gaacgtggga aaatcatgaa tgatgtcagt cgtgtcgcaa atgaattcat tcaaaccatt
     1681 tctaatgaaa tcaatcaata cagagaaaga gaaccagaga tccagccagc agaagtgaag
     1741 caggagcata aaaatactgt agaaacggca gaacaaaaga atattgtgcc cgttggctca
     1801 ctttggatcg acaagaaaga cgggaaagtc ctggaagaag acacgggccg aacgttacat
     1861 ttgaaggatg cggcgtttat tgaagatccg cagcataatc aagtgatttt aaaaggaaaa
     1921 agttatggaa aagagatcca aacggcgtta ccttcagaag aatttgcgct aggaaatcca
     1981 ttccgatttt cacttgagac agtgtcagaa gtggcaaaac gaaaaaatga aatctattat
     2041 gaacaacaat tgtcacaaaa agaacgcgta taaaacaagc aagaaagact atctgaaaaa
     2101 tggatagtct tttttctttt gggaggtttg aaaatgacag agaaaaaaat gagtctcatt
     2161 gatcgatgca agcaaatgga tattgtggat tttgcacgaa acaatggttt agcggtggcc
     2221 aataaaggac gagactatcg cttagaagac catgattcat ttgtttttga tcgaagaaaa
     2281 caacgttttt attggaacag tcaaaatatt agtggcgaca ttattgaatt agctaaactt
     2341 tttttcatag acaaagagat ccaagaccct aagcaacaat ttaaagcagc cactgatttt
     2401 attctaaaga atgaagacaa aacagaacga gtggaaaatc ttcattttga gacagaaaag
     2461 tacaaagatc accccgttga ttatcaacca ctcacagaaa aaggaaggaa ttatctgaag
     2521 gaagaacgaa aactgcccga ttggttgatt gattatgc
//
LOCUS       DACDNO010000148         2423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDNO010000148 DACDNO010000000
VERSION     DACDNO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.155"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            123..2276
                     /locus_tag="IWI22_002855"
     CDS             123..2276
                     /locus_tag="IWI22_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9170164.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVLVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          828 a          425 c          536 g          634 t
ORIGIN      
        1 caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct
       61 tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg
      121 aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa
      181 aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa
      241 attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt
      301 tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg
      361 tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga
      421 ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc
      481 ttgaaatcgc aacgaagctg aactcgctga tcgatgttct ggtagatgaa aatctgacag
      541 atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa
      601 gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata
      661 aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta
      721 aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg
      781 atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca
      841 cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga
      901 cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt
      961 ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag
     1021 gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag
     1081 aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat
     1141 caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac
     1201 agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa
     1261 tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga
     1321 ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg
     1381 caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg
     1441 cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag
     1501 catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag
     1561 actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat
     1621 tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg
     1681 aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca
     1741 aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata
     1801 caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc
     1861 cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga
     1921 aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc
     1981 tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat
     2041 tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag
     2101 gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc
     2161 cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact
     2221 tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa
     2281 gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc
     2341 tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact
     2401 cggcaaaaaa ggttgtgaca aaa
//
LOCUS       DACDNO010000149         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDNO010000149 DACDNO010000000
VERSION     DACDNO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.156"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            206..2068
                     /locus_tag="IWI22_002856"
     CDS             206..2068
                     /locus_tag="IWI22_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9170165.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2398
                     /locus_tag="IWI22_002857"
     CDS             2068..>2398
                     /locus_tag="IWI22_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9170166.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatt
//
LOCUS       DACDNO010000150         2374 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDNO010000150 DACDNO010000000
VERSION     DACDNO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.157"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(473..1144)
                     /locus_tag="IWI22_002858"
     CDS             complement(473..1144)
                     /locus_tag="IWI22_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9170167.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLAENYDNHVVIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGY
                     SFILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEM
                     FINAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPNTIYFKRV
                     GGGIT"
     gene            1667..2257
                     /locus_tag="IWI22_002859"
     CDS             1667..2257
                     /locus_tag="IWI22_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9170168.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT          768 a          399 c          364 g          843 t
ORIGIN      
        1 agagctaatc aaactaaaaa ataccccaag tcttcttatg taaaaacacc ggttattact
       61 taaatatgac ttaggtaaaa caatgagtga tccaattata aaattttatg gcgaataatt
      121 tagtaagcgc tacatattga atgatttaga aattttgaaa aagtagctag ttccttttat
      181 accagaaaag taaacctaat aaaaaacaca actaattttt agctgtgttt tttaagttac
      241 aataattatc tatttaatag tcttaattca ttaaaacaaa aaactcaact ttacaaggat
      301 atggtttaaa aaatttattt atccaaccca actagttttc gctgcttacg atttgcttca
      361 tctgctacgt acttcattac tgttttaaat tctttcgctg aggcgttttc tgataaatca
      421 gcgatacttt tattatactt cttcacgatt tgttgtactt gttctttctc agtcatgtaa
      481 ttcctcctcc aactcttttg aagtatattg tattgggtag tactaattga atattatctg
      541 ctgcataatg cacttcagaa atgtcgtttt gataatcctt aatcattatg tgaaggacta
      601 cttcaggatg tctatttttg actttttcaa tattctctct ggacttaaaa aacgcattga
      661 taaacatttc ttttggcaca tgacgtcctt cagctagttc acgttttcga gtgaaatccc
      721 aggcaacttt gggatcttgg taaacataga aaatcgtaac gcgaaagtta tttttaaggg
      781 ctcgaataat atttttctct gcactaagga tagcaaaagt atcctctagt atgaaagaat
      841 agccatgttc cagtacatat tttaatgatt gttcaactaa ccaagaagaa gctttctgaa
      901 aaacacttga gttctttcca ttgtattctg gaaattgtgc tctaaaatag tccgcatcaa
      961 tcacaacatg gttgtcataa ttttcagcta aacctagtgc aacctctgtt ttacctgctc
     1021 ctgggcttcc agccatgaat accgcatctt tttcatcatc agggattttt cccttgatga
     1081 ttgtttcaat aaatttatct ttattgaccc tagcgtaatc taaatacttg tctttaggtt
     1141 ccaagattgc accttctttc cttcttctat attcatacta attatacgat atttatataa
     1201 aaaatataac ggtcatttta accatattaa catttagtca cagtaatagc agatatgctc
     1261 gcaaaatgag atatgaatgt acgcagaatc cttgttttct acggcattcg ctgttttttc
     1321 attggactag ttatatattt ctagggttgt tttcatgtct gagtgcccga tatgatttat
     1381 ttttcgtttc tataattact ttttgccttc cagtttcttt taagattgta ctcaagcaag
     1441 tgggaattgg ttcttttgtc atccatgtat gcaaaatgtt ataatgctgg taaacaaaaa
     1501 tgattacctg tcatttttat ttagttcgta tttaattaag ttgactgtct gtatactctg
     1561 tctaaaactt aagaattgtt gttttcgctg tcgctatcca aatttaatga atgtctgtat
     1621 gttaaaactg ttgtccttgt catagatagt aaaggagagc ttgaaaatga aaactgcgat
     1681 tacgtttcta ttagaggaat atgccgattg ggaaggtgct tatattacta gcacactaaa
     1741 ccaaaaagag ggatggcaaa acaaaactgc ttctatccaa tcagaggtca cttccattgg
     1801 aggattaaaa acaaaaatcg attacttaat cgaacaaata cctgaaaaag ttgacctttt
     1861 tattatgatc ggtggcaatt catgggacat aaaaaatcat catttatatc aaataatcga
     1921 taaattgtta aaaacagacg taccagtagc tgctatttgt ggggcagttg attatttagc
     1981 tcagagcgga ttattgcaag gttacaaaca caccggaaat gcgcaatttc cttggttaga
     2041 atcggaaaat tatcagaata aagaagattt tatagaacaa caagctgtga aagatcgtaa
     2101 tttgattact gcgaatggaa cagctcctat agaattcaca gacttgattc ttcaagcagt
     2161 ccattttgaa acagaagaaa taagagaaaa aaagattcgt ctatacaaac ttggattcta
     2221 tgattactat aaaaagtacg gagatccttt ctcctaatca ttctcttcaa ttaaatactc
     2281 cttatcaata gtatgacaag taattttact gatggttatt tgtcacactt tttctgacat
     2341 ttatcaaaga agatacactc tatttatatt cata
//
LOCUS       DACDNO010000151         2370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDNO010000151 DACDNO010000000
VERSION     DACDNO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.158"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(34..399)
                     /locus_tag="IWI22_002860"
     CDS             complement(34..399)
                     /locus_tag="IWI22_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170169.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            607..1089
                     /locus_tag="IWI22_002861"
     CDS             607..1089
                     /locus_tag="IWI22_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170170.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1286..2176
                     /gene="pstS"
                     /locus_tag="IWI22_002862"
     CDS             1286..2176
                     /gene="pstS"
                     /locus_tag="IWI22_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP9170171.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          807 a          395 c          473 g          695 t
ORIGIN      
        1 agtagcttct ctttgtacat atatggtttg tttttatgga tgccgagctt ttttataaga
       61 taaggctacc gcccataacg acccaaggat ttgtggaact aagtaaatac ctgcaagaag
      121 caagatcagc tgggtcggtt ttaaaggatg atagaagtaa cgccattcag taaaattggc
      181 gcaaaataaa taggaagtga cagtagatat aatattggca atgaataaag atataatacg
      241 cctgttcctt tgaaaataat atccagcaaa aagagataaa aatatcaatg tgatgaaccc
      301 gcttacggaa tgataacgaa aatcaagcag taaggcgaaa tgcacataag gaacaaaaga
      361 taaaagcaac ggaaggtttt tccagcaagt ttttatcatc tttcatcacc acctaacaag
      421 tgttattatt ttgttagttt tattgtaaat tgaatcctgc aataatcaat tactttttca
      481 ttatttctag attaaatgaa taaactctca tgccattatc tctaaaaaaa aagtttttcc
      541 ccctaatttt tttagataga aaattgtata gtatgatgag agaaaaagta gtttggggga
      601 acgttaatga gaagaaaagc agtaatcgat cggctttatt tatttttttg gttttttcct
      661 atcgttttgc taccttttac atgggtaaat gctgttgtac aaggtgcgga gatattaggc
      721 ctgatccaag gaagcggatt tgtggtttct aggaattttc cagctttgat tgttttctat
      781 ttagtgataa gtgtaggtct cttattacca aaatctctga tcaatcacta caattggcag
      841 ctgctttact tttcttgctt attagtattt acatctatgt tttctttgct gcctcgcttg
      901 attatcggcc ctaatggttt gagcgagagt ggattgaagg atttagctta ttcctccatg
      961 tatttcttga cgatcaaacc atggttctat ttagtaattg cctcatttat tcttgcaaca
     1021 tttttttact tctggacaga aatcaggata aaaagtgaac gatctgtatc tataaaaaat
     1081 aactattgat ggtggatcat cagtaaaaaa acatatgagc tgcgtaaaat gcatgtttct
     1141 catatgcttt ttttattatt atgtaaagaa tgcgaaaaac gtttacatga tttttacata
     1201 acttaactat atttttacag caaccatacg agggatttac agtcgactga taaactttag
     1261 ttgtaaataa gaaaggagca ataaaatgaa aaaagtattg tttactctag gtatgatggg
     1321 aattctttta gccggatgcg gctcaggatc aggaaataca gatggctctg ccacacaaaa
     1381 taattcaagt gactcaaatg aacaagtaaa aatcgttgcg gtaggttcga cagcattaca
     1441 gccgttagtc gatgcagcac aagaaagctt tgtacaagaa aatccaaatt atcaaatctc
     1501 tgtacaagga ggaggaagcg gaactggttt gagccaagtc gaagctggag cagttactat
     1561 cggaaactct gatgtatttg cagaagaaag agacggagtt gatgcttcaa aattagtgga
     1621 tcataaagta gcagtggtcg ggatggcacc aatcgtcaat aaagatacag atgtcaaaga
     1681 tatcaccaaa caggaattga ttgatatctt tacaggaaaa atcactaact ggaaggaagt
     1741 tggcgggaaa gatcaaaaaa tcaacgtcgt gaaccgtgca aacggcagcg gtacccgcgc
     1801 gacattcgaa aaatggggac tggatggtgc tacccctgtg cagtcccaag aacaagattc
     1861 atcaggaaca gttcggcagc tggtaagtca aacaccagga gcaatcagct atttagcttt
     1921 ctcatatctc gatgattcca cacaagcact aagtatcgac ggtgtagaac caaaagaaga
     1981 gaatgtagca gataacagct ggggaatctg gtcatatgaa catatgtaca cgaacggaaa
     2041 accttcccct gaagtccaaa aattcttaga ctacatgatg acagaagaaa tccaagaagg
     2101 acctgtcaaa gaattgggct atttaccaat cacaatgatg gaagtagaac gtgatcacga
     2161 aggaaatatc aaataactag cagataaaca tgccacggta gtcgttacta ttaaggaaaa
     2221 atcaactatg tttccccaca tatacatttt tcctttaatt gtgaaaaata gttcctcata
     2281 ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tattcgaaaa
     2341 aaagagctac gacatagaac tgtcgtaact
//
LOCUS       DACDNO010000152         2293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDNO010000152 DACDNO010000000
VERSION     DACDNO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.159"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(51..1598)
                     /locus_tag="IWI22_002863"
     CDS             complement(51..1598)
                     /locus_tag="IWI22_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9170172.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IWI22_002864"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IWI22_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9170173.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IWI22_002865"
     CDS             complement(2043..2237)
                     /locus_tag="IWI22_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170174.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DACDNO010000153         2230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDNO010000153 DACDNO010000000
VERSION     DACDNO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.160"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(363..1655)
                     /locus_tag="IWI22_002866"
     CDS             complement(363..1655)
                     /locus_tag="IWI22_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9170175.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1929..2189)
                     /locus_tag="IWI22_002867"
     CDS             complement(1929..2189)
                     /locus_tag="IWI22_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170176.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          731 a          425 c          343 g          731 t
ORIGIN      
        1 tgggctactc tgtgatactt attagccatt aaactatttt acccacatag catatatttt
       61 gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa atctaagata
      121 gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac aactttaaaa
      181 aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg tttaatcatt
      241 catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc caaaaactat
      301 gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg gctattttga
      361 aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt acggaaggta
      421 aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg attaagcacc
      481 atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt cgtgcgtaaa
      541 taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt gacttttctg
      601 aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg aatggcacta
      661 ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc tacagcacca
      721 gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc gtttagagaa
      781 gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt acttggaata
      841 tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac cgatggaaag
      901 atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa aaagacatct
      961 tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt agattctgtt
     1021 tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc aagaatggtg
     1081 tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat acaatcaacg
     1141 aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa ttcaggaagg
     1201 acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata aatagcttcg
     1261 acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag aaccttccct
     1321 tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa ggaagcttcc
     1381 cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt tttagttcca
     1441 tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc ataacctaaa
     1501 tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac atctttcaaa
     1561 gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga ttcagctaac
     1621 ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct tgatcttatt
     1681 gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta cttacacggt
     1741 ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca atgaaattat
     1801 ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt tgatcctatt
     1861 atccatatct ttttcagttt taatattgat acacattata agaaaaagaa acttttggtt
     1921 aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg caatagatta
     1981 acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc aaaaagttct
     2041 aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag attagaaaat
     2101 aacaccccga gccaatctgt gaaaattact atatttacta atatccctat taacatacct
     2161 atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa atctgaagta
     2221 cttatattaa
//
LOCUS       DACDNO010000154         2195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDNO010000154 DACDNO010000000
VERSION     DACDNO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.162"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            238..1695
                     /locus_tag="IWI22_002868"
     CDS             238..1695
                     /locus_tag="IWI22_002868"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HAP9170177.1"
                     /translation="MKKNQPNVVLIVVDQMRADALSLNSQDKIISTPTLDMMASQGYN
                     FENCYSPVPSCVPARAALLTGLDQETSGRVGYEDEVPWNFKNTLPEVFKEQGYQTECI
                     GKMHVYPSRKRLGFDHVLLHDGYLHVDRKYDKSYGEQFEYSSDYLMFLKESLGSDADL
                     IDDGLNCNSWEARPWMYPEKFHPTNWVVSEGINFLRRKDPTVPFFLKLSFEKPHAPLN
                     PPKYYFDMYMDRLPDTLDLHIGNWEKLEHVVPDVCALRGKLKEDDQRRMLAGYYGLIS
                     HIDHQINRFLMALKEFRHDKDTIIWFISDHGDQLGEHYLFRKGYPYQGSIRIPSFIYD
                     PGDLISAKKHGIKELVKIQDIFPSLVDLVLGQYVNTDGKSVKQLLFGNCEGWRREIHG
                     EHSLGLDSSQYILTEKWKFIWFPVKNTYQLFDMINDPNEMKNLYYDKKYESIIYEMKH
                     KLVGYLKGREEGFVKNGQLIQIGISNIVSTLKSKK"
     gene            1886..1963
                     /locus_tag="IWI22_002869"
                     /pseudo
     CDS             1886..1963
                     /locus_tag="IWI22_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          806 a          261 c          415 g          713 t
ORIGIN      
        1 agaagatatt agagatttaa tgaataataa gttatataga taaaaaatta aagatatttt
       61 gatatcaagg tttattttag tataaagtat tcgtgactaa tcaatcatac gctataaagt
      121 ttggtaatcc atttatattt cttgttatca caaaaaatat attctttcaa aaggaaatat
      181 caatttctta aatcaagaga gattatttgg atattttgaa tgattggagt ttttaatatg
      241 aaaaagaatc aacccaatgt tgttttaatt gtagtagatc agatgagagc agatgcgtta
      301 tctttgaata gccaagataa aatcattagt acgcctacat tagatatgat ggctagtcaa
      361 ggatataatt ttgagaattg ttattcgcct gttccaagtt gtgttccagc aagagcagcg
      421 ttattaacag gattagatca agaaacttcg ggaagagtgg gttatgaaga tgaggttcca
      481 tggaatttta aaaatactct tccagaagtt tttaaagaac agggatatca aactgagtgt
      541 attgggaaaa tgcatgtgta tccttcaaga aaacggttgg gatttgatca tgtactattg
      601 catgacgggt atcttcatgt tgatcgaaaa tatgacaaat cttatggaga gcagtttgaa
      661 tattctagtg attacttaat gtttctaaaa gaatcattgg gctcagatgc agatttgatt
      721 gatgatgggt taaattgtaa ttcatgggaa gcacgaccat ggatgtatcc tgaaaaattt
      781 catccaacaa attgggtggt ttcagaagga ataaactttt taaggcggaa agatcctaca
      841 gtaccatttt tcttaaaatt atcatttgag aaacctcatg ctcctttgaa tccaccaaaa
      901 tattattttg atatgtatat ggatagatta ccagacactc tagatttgca tattggtaat
      961 tgggagaaat tagaacatgt tgttccagat gtttgtgctt taagaggaaa attaaaagag
     1021 gatgatcaaa gaagaatgct tgctggatac tatggcctta tttctcatat agatcatcag
     1081 atcaatcgct ttttaatggc tttaaaagaa tttagacatg ataaagatac aatcatttgg
     1141 ttcatttcag atcatggaga tcaattagga gaacattact tattcagaaa agggtatcct
     1201 taccaaggga gtattcgtat cccgtcgttt atttacgatc ctggtgatct gatctcagca
     1261 aaaaaacatg gaattaaaga gcttgtaaaa atacaggata tatttccttc attagttgat
     1321 ttagtattag gacaatatgt aaatacagat ggaaaaagtg ttaaacagtt gctctttgga
     1381 aactgtgaag gatggcggcg agaaattcat ggagaacata gtttagggct ggattctagt
     1441 caatatatat taacggagaa atggaaattt atatggtttc ccgtaaaaaa tacgtatcaa
     1501 ttatttgata tgataaatga tccaaatgag atgaaaaacc tatattatga taaaaaatac
     1561 gaatctatta tttatgaaat gaaacataaa ttagtaggat atttaaaagg tagagaagag
     1621 gggtttgtca aaaacggaca gttaattcaa ataggaattt caaatatcgt ttcaacttta
     1681 aaaagtaaaa aataaactaa aagtgaagat gcaataaaaa atcaacatag ggtatactga
     1741 gtcaaaatat actcagatac aggcaaaact ttgcgtcttt atttatttca aaaaaatcaa
     1801 atattcggat ggtaacaaac atactacttt gtttgggata aaatcagatc tgagtcagag
     1861 taagttcatt ctgattcagt tttttatgtc taacgacgat aaaagtttag cacatacaag
     1921 atggaattgt aagtatccca tagtctttac cccggagtat cgatgaaaag tgatttatgg
     1981 aaaattaaga gtaagtaact gttggtgata gtatcacccc aaattagtat ttccagtttt
     2041 atgagctatt tattaaatga ttaaaaagag atgagggtgt gagctgttac tgatacttta
     2101 aaaaaaactt ttaaagtact atcaagtgat tagctcttct agagaggtta aaaagaattt
     2161 gttttctaga gtagatatta cttgagagtg taaaa
//
LOCUS       DACDNO010000155         2170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDNO010000155 DACDNO010000000
VERSION     DACDNO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.163"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            382..>2170
                     /locus_tag="IWI22_002870"
     CDS             382..>2170
                     /locus_tag="IWI22_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170178.1"
                     /translation="MKRFLKIFIFLEIILFVYIFNTSIYNIYEKNNIATDNLKGYVLE
                     ETSPEILDKFYTIFTEEYSQNKLELINNKLTSTDKSVYDLYCYPLNEFVQKQPISNSI
                     LLQYHKLQKEDFLDSVGVFYTDLQANEIREIESQLAVTISVFENEAIPYSMILELYLL
                     NFVILFVVLQIIYCIYTSDSLKKKGIKKSMGFSAMHILKEQINNIVKYFIVICSVLFI
                     LLNLYYALTNRFNISYLTISIFFFAIILVINILCVLITSFLIKFVSLEAMIKNKTLNS
                     GTNLIVQTIKIVFSVIITITTISLLNQGNEFKQSQQDILDYKYLDGYFTANGFNSLEY
                     EYALENPEQFEEYSNSMLELYNNHHALLCDYAGSSEAQGEFSKSRPYYIQQTIIANRN
                     YISEFSNIQVNGKSLNEDIYDTPTILVSRKYKSDETFIKEHITQEYFRLMNYNQFYGI
                     PEKERHINEFNIVYVDDNSTIKVNTENGFSDMSEPIIIIDTGNFAGLYYLDSLNTRNL
                     FFQMDSREEFSSLLAEYGLKQLVTAGTLLTPYLMQLENVTFVLKTLTMFAIVFIISLL
                     FILYISNYVDIFVNRKKYATKEIMGFSHFK"
BASE COUNT          769 a          278 c          341 g          782 t
ORIGIN      
        1 tcgatttctt gaagtgagta aggaatgctt tgaatggccc tttttacaag ctgcgcatct
       61 tttttatttc cgcaagaatg gccaataatt tcacgattga ataaatcaat catcaaacaa
      121 atataagccc aatttgtccc gacccgaaca taagttaaat ccgtgacaac cgctgacaat
      181 gcttttgttt gagtaaactt tcgatctaag tgattcgcaa ttttcttttg attgctttgg
      241 cacgatcata aagacgggag cagatattgt ctattgatat aatgatagta gacaaacaga
      301 gttggctgat tagctaaagc atatcgagta taatttgaat attagacgca tgatttatca
      361 gtgtaagaaa gggtgaggat tatgaaaagg tttttgaaaa tatttatctt tctcgaaata
      421 atactttttg tatatatttt taatacttct atttacaata tttatgaaaa gaataatatt
      481 gcgactgata atttaaaagg atatgtttta gaggaaacat cgccggaaat tcttgataaa
      541 ttttatacga tatttacaga agaatattcc caaaataaat tagagcttat caataataaa
      601 cttacttcta cggataaatc tgtctatgat ttatattgtt atccgctaaa tgaatttgtg
      661 caaaaacagc ctatatcaaa ttctattctg ttgcagtatc ataaattgca aaaggaagat
      721 tttttggata gtgtcggggt attctatacg gatttgcaag ccaatgaaat acgtgaaata
      781 gaatctcaac tggctgtaac aattagtgtt tttgaaaatg aagctatccc gtatagtatg
      841 atattagagt tgtatcttct taattttgta attttgtttg ttgtacttca aataatatat
      901 tgtatttata catctgatag tttgaaaaaa aaaggtatta aaaaaagtat gggcttttct
      961 gctatgcata ttttaaagga gcaaatcaac aatatagtaa aatattttat tgtgatatgt
     1021 tcggtgttat ttatcctgct gaatttgtat tacgcattaa caaatcgttt taatatttct
     1081 tatttaacaa taagtatatt tttctttgcg attattttgg ttataaacat actttgcgtt
     1141 ttaattacca gctttttaat taagtttgtt tctttggagg caatgattaa aaacaagact
     1201 ttaaatagcg gtacaaatct tatagttcag actataaaaa tagttttttc agtaataatc
     1261 acaattacca ctatttcatt attgaatcaa ggaaatgaat ttaaacaaag ccaacaggat
     1321 attttagatt ataaatatct ggacggatat tttacggcta atggttttaa ttctttagaa
     1381 tatgaatatg cattagaaaa tccagaacaa tttgaagaat attctaattc gatgttagaa
     1441 ctgtataata atcatcatgc cttgttatgt gattatgctg gtagcagtga agcacaaggg
     1501 gaatttagta aatctcgtcc atattatata cagcaaacaa taattgcaaa tagaaactat
     1561 attagtgagt tttcaaatat ccaagttaat ggaaaatcat taaatgagga tatatatgat
     1621 acgccaacaa tattagtttc ccgtaaatat aaaagtgatg aaacttttat taaagagcat
     1681 ataactcaag agtattttcg actgatgaac tacaatcaat tttatgggat acctgagaag
     1741 gaaaggcata tcaacgaatt taatattgtg tatgtcgatg ataattctac tataaaagtt
     1801 aatacagaaa atggcttttc agatatgtca gaacctatta tcattattga tacaggcaat
     1861 tttgcaggtc tttactattt ggattcttta aatacaagaa atttattttt ccaaatggat
     1921 tcaagagaag aattttcttc tttattagca gagtatggtt taaaacaatt agttacggca
     1981 ggaacattac ttacaccata cttaatgcaa ctggaaaatg tgacctttgt ccttaaaaca
     2041 ctcacaatgt ttgcgattgt ctttataata tcattactgt ttatcttgta tatttcaaat
     2101 tatgtagata tttttgtgaa tcgaaaaaaa tatgcaacta aagagattat gggattctct
     2161 cattttaaaa
//
LOCUS       DACDNO010000156         2072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDNO010000156 DACDNO010000000
VERSION     DACDNO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.164"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            3..1085
                     /locus_tag="IWI22_002871"
     CDS             3..1085
                     /locus_tag="IWI22_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9170179.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1303..>2072)
                     /locus_tag="IWI22_002872"
     CDS             complement(1303..>2072)
                     /locus_tag="IWI22_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9170180.1"
                     /translation="IHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLEIIEENPVDT
                     IGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVRSEELLALQW
                     KDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLKTWKKTQQSI
                     GKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFLIEHNKNIYA
                     ISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
BASE COUNT          703 a          369 c          352 g          648 t
ORIGIN      
        1 atatcaatga tgatggctca agaaaaaaag tggagaaagt tggcggagac acccgaccag
       61 aggccgaagc tgctttacga aaagttttat cagatattga cgaaacagga caatactttt
      121 taggtacgga tactcgagta aaacaatacc ttgatttttg gatggaggaa tacgttaaac
      181 taaatctaaa atacaatacc tatgaaaact accgatttac catcaaaaat catataaacg
      241 gttatttagg aaagaaaaaa cttacggatc tctcccctgc tcttttacaa aatttcatca
      301 atgctgaatt taaaaagggt tactcgaaga aaacaatgac tattactcac tctgtcctta
      361 agaatgcgct gaatatggcg gtttatcctt ggggattaat caagcaaaat cctatgctgt
      421 atgtaaaaat accaaaatat gaagcacgac caacgactaa aaaagatcta aaaatcattt
      481 ctcttgagga ctttgatcat atgctagaaa ttactcctga aggacatcct ttctatattc
      541 ctttgaatat tggattttat acgggaatgc gcgttggcga agtttgtggt ctgacgtggg
      601 atgatgtcga tttttcaaat ggaacaatta ctgtagagaa acaaatggta aagaatgacg
      661 gcgcatgggt atatggtacg ccaaagacaa gcagttccaa tcgaacgatt tttattggac
      721 aaaccttgct agcaattctg aaaaaacata agaaacaaca attagaaaat cgaatgaagt
      781 atggaaagct ctacattgat tcaaatgcag tatgtacgaa ggaagacggt gagctagtta
      841 cgccaagtgt agtgaaatgg aatactagga ggatatcgaa tgcactctcc ctctctttta
      901 acttccattc tctcagacat actcatgcta cacttcttct cgaaaatggc gcaaaaatga
      961 aagaaatctc tgaacgattg ggccacagca gaatttcaat tacgatggat acttactcgc
     1021 atgtaacaga taagatgaga aatgaaacgg tcgatatcat ggaaaatctt agaaagaatt
     1081 cttgattttt gccaccgaaa aaaaccaccg gtggcaaacc ggtggcaaat ccatgtaaac
     1141 atggtttatt ttttacactg ttttcaaaca aacccttgct atttcttcgg ttcgtcctcg
     1201 tccattttcg ataccgacag ctagcttata acccaaggta tatcaacata aaaaaataaa
     1261 aataggctat gacagcagcg tgacagcaaa aaagatggaa agttaaatat caaacctcgt
     1321 aatttcattt gcgagtgctt gatttgtttc tggatataag tgtccatatt tgtccaaagt
     1381 agttttaata gaggcatgac ctagacgctt agatattgca tagatatttt tattatgttc
     1441 aattaaaaag gcaacatgcg agtgtcttaa tccatgcata tgaattggtt ttactcctgc
     1501 aatttcagct agttttttta ttctgcttaa aatagttctt ggagatggcg gtaatccatc
     1561 aaaagaaaaa accaattcta tatttttccc aattgattgt tgagtttttt tccatgtttt
     1621 taaatcttca acggtttgtc tgtctaacga aattatccga atacttgatg tattttttgt
     1681 ttcagcaaat tcataatctt gtcttgtacg gatgtaaatt gacttattta cgctacatct
     1741 gccatttatt aagtcaaagt ctttccattg taacgctaag agctcctcac tccttacacc
     1801 tgtaacaaaa ataaaacgca tcaaacgttt ataaaattct tcataaaaat ccccgtgata
     1861 aacactatta tatactttct taaattcatc aactgtccaa aattctactt gaggacgctg
     1921 agtttttatt ttacctatag tatctacagg attttcttca ataatttcaa gtacaattgc
     1981 ccttttaaaa actactctca acttattgaa tatctgttta atataatttg gtgataatgg
     2041 ttgctcaatg ccctttttta caacatggat tt
//
LOCUS       DACDNO010000157         2011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDNO010000157 DACDNO010000000
VERSION     DACDNO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.165"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..334)
                     /locus_tag="IWI22_002873"
                     /pseudo
     CDS             complement(1..334)
                     /locus_tag="IWI22_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            609..1151
                     /locus_tag="IWI22_002874"
     CDS             609..1151
                     /locus_tag="IWI22_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170181.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            1307..1756
                     /locus_tag="IWI22_002875"
     CDS             1307..1756
                     /locus_tag="IWI22_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9170182.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1786..>2011)
                     /locus_tag="IWI22_002876"
     CDS             complement(1786..>2011)
                     /locus_tag="IWI22_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9170183.1"
                     /translation="ESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLA
                     YETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          697 a          298 c          390 g          626 t
ORIGIN      
        1 ttgcacatcg ctgatatgga atatttatgc ttgttggcat caattacttg tcttttcgtc
       61 cgaatatcag cgccgcttgc tttaaaatat cattctccat ttcgagttgc ttatttttct
      121 ttcttagtgc gatcaattct gcttgttctg gtgttaagtt gtctctttct ttgaatgagc
      181 ccgttgattg tgcttgcttc atccatttat cgaaagaaga aggcgtaagc tcatactctc
      241 gaataatttc tgcgcgaggc ttaccagcga gatagagatc gacgatttgt tgtttgaatt
      301 cttttgaata ggttcttcgt tgacgtcttg acatgaaaaa tcctccagtg tgttttttat
      361 tattctacac accttatttt ttctgtctag tctagtgtag ccgattcaaa ttgatgaagc
      421 agatcccaaa gggctgcttc atcaattttt tcgtaagtgt ccgtacactt actgtgcttg
      481 ctcatatatt aatcgatcat atactaatcg tgtaagaaca actttaagca taatacttat
      541 attgttatac catagtttgg aaaaataaaa aattatattt catcaacgtt tcataaaagg
      601 tgtgtagaat gttattgaaa gtagctataa aatgctattg tgtaaaaaat aaaggaggaa
      661 taatttatga aaaaatagta ccaagaggag catgcctatt acttgcatta tgtttgagct
      721 taagcgcagt tgggtgtcaa aaaagtaatg aaaacaataa aataaaagaa ggacaatcca
      781 taagttctga agaggaaggc atgtcaatta atgcaaatgg caaaaatgaa acttttaagc
      841 catcagatta tacattggag gcaaagaaag aatatgttta tgaatattta gggcttaaat
      901 ttaaactctc agacaaattt agaaattata tagctgataa aaagatagcg atgttagatg
      961 atcaaagtcc tatagataaa gaactaaagt atgctatttt gacatttgag aaaatgacag
     1021 aagaacaaaa gaatgcagtt atagaaaaga tgggcgatgg atataaaaat tggcaaaatg
     1081 agcttgaaag aattgggact attggtatat ttgaaaaaaa tacatcagaa gaaaaaaatc
     1141 taaaattata aaatgtgata ctcatactaa aataggagtt tcaagtgatg gaaaaaacga
     1201 ttgctattta agcacaaata gcggagctga aagtaatctt ctggatgaat taaaaagaac
     1261 tgagattcaa attatagata aaaaagaacg tcctaaaaat ggttttgtgc tatcagaaaa
     1321 aactgattta gaaaatacag aagcatttaa caaagaatcg gttaaagatt tacgtaaact
     1381 accaacaaag gacataaatg gcaaggattt tacaagtaaa gattttgaga agtatgatct
     1441 tactatggta aatgtatttg caacttggtg tacagcttgt gtaaaggaaa ttcctgattt
     1501 ggttgaggtt cagaatgaaa tgaaaagcaa gggtgtaaat atcgttggag ttgttacaga
     1561 tcctgttgat gataacggtg aaaacaagga agcaattgaa aagtcaaagt taatacatga
     1621 aaagacaaag gcttcatatc ctttcttaat gccagatgga atggcaacac tttttttgac
     1681 aaaaattata aggtgttgtg aatgctgcgg tgttatggct acgattgccc ttgacaatga
     1741 gcatcctcgg atctgatttt ctccggcaga agcacaatca ttcaattacg ctgcctctcc
     1801 agaggtatca gatccgcgct cattatcaag gattcgcttc gccaatctct gttgtcgaat
     1861 ttggatcggt gtttcatacg ccaacgtgcc atgtaagcgt agatagttcc accaatgcac
     1921 ataatcaaac aattctagac gtagctgtgc cagtgtctca aattggtatt gatgcacgaa
     1981 ttctactttg acagatttat aggttgattc c
//
LOCUS       DACDNO010000158         1995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDNO010000158 DACDNO010000000
VERSION     DACDNO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.166"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            128..1852
                     /locus_tag="IWI22_002877"
     CDS             128..1852
                     /locus_tag="IWI22_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP9170184.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          783 a          242 c          348 g          622 t
ORIGIN      
        1 cagtttgtaa caaacatata ttttttatat tacaatattt caacaaatta aaaaaaatta
       61 tatcttctta aacaatgata atattaaaat gcaagcgctt gtaaaaaata aggagaagag
      121 gtaaaaaatg aagaaaaaac tattaacttt atttcttgtg atgtctttct caatcgtttt
      181 gagtgtgtac tacaatacta ttgttttttc agaacaaata aactacacag aagaacagta
      241 taatatagaa aaagaaaaag ctttaaaagt tggttatagt gaagaacagt ttaagcagat
      301 tatggaaata cctacaaatt tatcaaatga aaatagtgaa acaagaatag taaattatac
      361 tatgacttct aatcaaacca aagtcattaa taaagcaatg gagcaaattg gaaagccata
      421 cgaatgggga gcatcgggac caaattcttt tgactgtggt ggtttagtga aatatgttta
      481 caaacaagct gtaaatattg aactgcctat gggaaccacc aatcaagagc aatacggtac
      541 agaggtaagt ttaaattcac ttaagcctgg tgatttatta ttttacggca atcgaggtgc
      601 aacttatcat gtaggtatat ataaaggtaa cggtgtaatg attcatgcac cgcaacctgg
      661 agaaactgta aaagaggtta acatacaata tttttatcct aactttgcaa aaagaatttt
      721 gcctgatgaa cctgactacc cttatattga ctataataaa atggtaactg ttacaaaagc
      781 ttggtcaata tggaatgatt tacaatttag tcatgaaatt aagaaagcta ttattggaga
      841 taattacaag atagggaagg tttatactaa tccagaaaat aataataaat atggagaaat
      901 attagtaagt aataaagtat atggatacat taatttttat gctgttaaag agttgacctc
      961 tgttcaaata aacagatatt taacttctaa agatagtggt caaccaactt ggggaaatct
     1021 agagtgtact ataagtaaag gacaaactac aaaagataaa atatattttg ttaaaggagc
     1081 atataattta ggggatggaa agtatctata ttctatttat aaagatcagg attcctctga
     1141 atggttaggg tatttaaaag cacatgttag tttagcctat actcctattg aagaaattaa
     1201 taaaaacgta actgttacta aaaattggtc gatttggaat aatctacaat gggaaaagga
     1261 aattgaaaag cctcagattg gttctgtatt ttcagctaga ttaaagttaa ccaatgtatc
     1321 gaataatgct gtctattaca agctttataa atctggaaaa ttttatggtt atatcaatgc
     1381 tgaggctgtc aaagacctta caacaacaaa gcttaataag tatgttacat ttagtgttaa
     1441 taatgaagat ttttggtctt ctttagatgt aaattattca aaaggaaaaa ctgaaagagg
     1501 ccgagtattt tacattagta tttcatataa taccgcagat aaccaaccga tttattcagt
     1561 atatacagat gaaacatgta cggaatggcg agggtattat aagggaaaca actttgaaga
     1621 cactcaaatt actatgcttg agaataaaag cgtgaaagta acaaaaagtg ggtatacagt
     1681 atggggagac ttgaattttt ggactaagtc aggaatatcg aacacaggag atatatacac
     1741 cactgaccga aaattttata attttaccaa caatgcttac tattatgaat tgaaaaaaga
     1801 tgggagggtc tatggctata taaatagtga agcagctgta gagatgaact aaaatattgc
     1861 tgtcccttaa tcttatgggg cagcaatatt tttatattat taatcatttt tctgaagcga
     1921 gggtagaaga attaatcaat agtttttctt gttttgtaat ataatagttt aattaccaaa
     1981 aattatttaa gtaaa
//
LOCUS       DACDNO010000159         1995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDNO010000159 DACDNO010000000
VERSION     DACDNO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.167"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(63..356)
                     /gene="rpmA"
                     /locus_tag="IWI22_002878"
     CDS             complement(63..356)
                     /gene="rpmA"
                     /locus_tag="IWI22_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9170185.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(369..713)
                     /locus_tag="IWI22_002879"
     CDS             complement(369..713)
                     /locus_tag="IWI22_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9170186.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(740..1048)
                     /gene="rplU"
                     /locus_tag="IWI22_002880"
     CDS             complement(740..1048)
                     /gene="rplU"
                     /locus_tag="IWI22_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9170187.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            1330..1971
                     /locus_tag="IWI22_002881"
     CDS             1330..1971
                     /locus_tag="IWI22_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9170188.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
BASE COUNT          602 a          436 c          351 g          606 t
ORIGIN      
        1 tttataaaca aaaaaggctg tgacaaaagt cttgtcacaa cctctttaat cataagattc
       61 aattaagctt cttgagctac tggataaaca gacacttgtt ttttgttacg gcctttacgt
      121 tcgaaacgta ctacgccatc aacttttgca aataaagtgt cgtctccacc gatacccacg
      181 tttacacctg gatagatttt tgtaccgcgt tgacggtaaa gaattgatcc acctgtaact
      241 gtttgtccat cagcacgttt agcacctaga cgttttgatt ctgagtcacg gccgttagat
      301 gtagaaccgc cacctttttt gtgggcaaat aattgtagat tcatagataa caacatggtc
      361 tgcacctcct atttatcatt aattgtttta atttggatat attctgagtt ttcagcttct
      421 atcgattgta atcctaataa aagattttct aatagaatct cagcaatatt gttttgttct
      481 tgtgtcattc ctgaagtgac ttcggtataa agataaccgc cttcttcttc attcatttca
      541 acaatcggct caaaaccggc taatgaggca atcccgttga cagtgctgat cgctaacgca
      601 gaaacacccg cacaaacgat atcacttcca taaggaccag catcagcatg cccggtcaaa
      661 gtgaatgaaa cgatctgacc tgcgtcattt cgcttaaatg ttcctttgat catatatagg
      721 ccttctttct caaacgctat taagcgttga tcgcgtcgat aaccactttt gtatatggtt
      781 gacgatgacc ttgtttacgg tgtgaatgtt ttttaggttt gtatttgaaa gtaacgactt
      841 tcttttgttt gccgtgtttt tcaacagtac cttcaacagt tgcacctgca acagttggag
      901 cgcctacttt tgtagattct ccgcctacta agataacttc gtcaaaaaca actttttctc
      961 ctgcttcaac gtcaagtttt tcaacataga ttgcttgtcc cacttcaact tttacttgtt
     1021 taccaccagt tttaataatt gcgtacatgc tataaagcac ctccttctga tatacttaga
     1081 ctcgccatcc gaagtgacag catatgctgt gcttattact tgttctgagc ggttgtagct
     1141 gtggagtcca caattacaac agtactagct taccagaaca gcaaccgtta gtcaactaaa
     1201 tttaaaatca aaaagtaaaa caaaaattca agcaatactt ctgcttttct atcctcacag
     1261 ttctaccaaa taaaatagtc gcattgtgtt tcttttattc ttataataaa agaaaaagga
     1321 ggagcataaa tggaataccc aaaagaactg atacaagaag cttctttaag aatgaacggc
     1381 cgtcctgtag aaggatttat cgctgggcaa ggacctcttc atcctaaact gatgctcgtg
     1441 ggagaagccc cagggaaaac agagattgat acgcatgttc cttttagcgg tcaagctgga
     1501 aaagaattga tgcaggcact ttcttcaact ggcttaacac gagaagaggt gtatatcacc
     1561 agtgctgtaa ggagccgccc gtatcgcgtg actcatcgga tcaataaacg gacacaacag
     1621 tcagaaactg tttatccaaa tcggacacct actcgttccg aagtatttgc gcatgcacca
     1681 atattggatt acgaactgat gaaggtccag ccaaaattaa ttgcgacact tggaaacatt
     1741 ggtcttcaac gtttacttgg caaagaatat acggttacca aaaatcatgg tcaactctat
     1801 acagggcctg tcttacaatt aacagaacaa aaggatgcct acgaatggtc agaaaaaaac
     1861 tatcgaattc ttccactttt tcatccagca gctattttct acaatcgctc ccttgtacct
     1921 gttatccatc aagactggca agtactagct tcacttttac agcaatatta aaaagaacag
     1981 acaagaccat aaaat
//
LOCUS       DACDNO010000160         1891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDNO010000160 DACDNO010000000
VERSION     DACDNO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.168"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            715..1800
                     /gene="xerS"
                     /locus_tag="IWI22_002882"
     CDS             715..1800
                     /gene="xerS"
                     /locus_tag="IWI22_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9170189.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          704 a          317 c          257 g          613 t
ORIGIN      
        1 aaaaccacta gcccgccagc tagtggtttt tggtgtatca tgaattacct tttcgttaaa
       61 acaattatac catatttcct aaaaaataca attaatagac gaattatcat taccactttg
      121 atgaaaaaag ctgacataaa tttttctttt atttttataa tgaataaaca tttatcaata
      181 agcttttgaa aggcaggatc gtactttgtt gatgattgag atattgctac atcaatctca
      241 gcagttaaga tttttttgaa ttgttgcttg aattttttct ttatctaaaa aaattgtatc
      301 tccaggatgc aacccatttt ctttaagaaa ttccttttaa attgttaata ctactgagtg
      361 ccccacttct ctgaattttc tttaacttct ccctcccctt ttttatttgt atatacattc
      421 atatacgctt gttaatatca aatcaaccgt agtctctctt atgtctaatt atatagaaag
      481 attgttcttt ctatataatt tagacaattt aaaaaattaa gattgcctcc ttctgtttcc
      541 tttcctattc atgtatacca tttattttaa attattaaat ctatacctat ttatctgctt
      601 ttttgaaata aattaataaa tttcagagta taccaattga caaaacactc tataattggt
      661 atagtccaat tatagagata ctcctctccc ctacttatcg aaaggatgaa taacatgaaa
      721 aatacagaaa tatatcaaaa aatagataca atcatacttc atatgcccga ttatataaaa
      781 gattttgttc aagatcgtga ggataaagat caatcacctc gaactacttt agaatatcta
      841 aaaaattata agctctttta tgaatggctt ctgagtgagt ctatcgttcc cgatacttca
      901 atcacctcga taagacagtt aactgcctat gatctaaact tgtacaaatc tcatttgaaa
      961 cgtcgcacaa aggaaaatgt aaaaaaagag acaacaaaaa ctaagctcaa agataataat
     1021 aacttaggtc tatctacttc tactatcaat agaaatatca cagcattaaa ggttctattt
     1081 aaatatcttt cgaaaagttc caataatcct ttaggaaaac catacttaga aaataatcct
     1141 atggatcaag tagcaactat cacagataaa actacgttag ctgcccgtgc aaacgcaatc
     1201 gagaaaaaat tatttctaga cgaagataca caaaattatc tagattatat tgccaatgaa
     1261 tataaaaaaa ctttgagcaa acgagctttg atctattatc aacgagatgt agaaagagac
     1321 ctggcgatca atgcattgat tttaggatct ggattaagac tctccgaggt tgtaaatatt
     1381 aatttagagg acctatcttt agaaaagaat aatgtggtag taactagaaa aggaaataag
     1441 agagacgcag taaatatagc agcatttgca atggaatatt tagctaatta tctagctatt
     1501 cggaaagaac gatacaaagt aacagaaaac gaaaaagctt tatttctagc aatttatcaa
     1561 ggagaagcca agcgtatatc cggtattgca atcgaacgga tggtagcaaa atattcaaaa
     1621 ggattcagag tccaagtcag tccacataaa ttacgtcata ctttggctac tagactctat
     1681 caacagacaa attcattggt attaactgcc caacaattgg gacatacaag tacaaataca
     1741 acaactttat atactcatat agacaatgca gctacaattg atgctttgaa cgctttgtaa
     1801 atgtagtaaa aaatagtata ataatgaagc ctgtctctaa aattagataa aagcccaact
     1861 ttaaaggcaa gcttcattat catttttata t
//
LOCUS       DACDNO010000161         1856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDNO010000161 DACDNO010000000
VERSION     DACDNO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.169"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            332..946
                     /locus_tag="IWI22_002883"
     CDS             332..946
                     /locus_tag="IWI22_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9170190.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(970..1320)
                     /locus_tag="IWI22_002884"
     CDS             complement(970..1320)
                     /locus_tag="IWI22_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9170191.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1314..1577)
                     /locus_tag="IWI22_002885"
     CDS             complement(1314..1577)
                     /locus_tag="IWI22_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9170192.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          643 a          318 c          271 g          624 t
ORIGIN      
        1 aataaactta tttttcattg tctatcacct cgaaatcaaa aataagaaaa cgttttctta
       61 ataaatctta acagataaat caagttatat actagaaaat tcataaaata tatttttaat
      121 gtcataatcg tttaaatttt cttgattgac atttatttac aaaaaatatt gccatttaaa
      181 aagaaaagtg acacatacta tttcctccat cattagtttt tgtctatata aggtataccc
      241 taactagaca cgaccgcgac attgaaaaat ctggttttct cgtgtatgat tagggtgttt
      301 tttaaatagt tgcagaaagt aggtaaaaat tttgagagtt gcatacatac gtgtctcatc
      361 catcgatcaa aatgaacaac ggcagattga agaaatgaaa aaattcggtg cagagagaat
      421 tttcattgag aaacaatctg gcgcaacgat tactcatcga cctgtttttc aagaagcact
      481 ggatttcgtc cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa
      541 ttacgacgag atcatcgctt cagtcaatta cttgaagaag aaaaatgtgc agctgatcat
      601 cacaagtcta ccgatcatgg ccgaagcaat tggcaatcct ctcctggata aattcattaa
      661 agatttgatc attcaaattt tggccatgat cgctgaacaa gaacgtaccg aatctaaacg
      721 acgtcaggca caaggcatta agattgcaaa agcgaacggt gtttataaag gacgtcctaa
      781 gctatacagt gccaatgcaa aagatcccca acgtcgtctt gtttataaaa acatcgttga
      841 ggatctaaaa aaaggtgttg cgatcgctaa gattgcaaaa gactacaatg ttacgagaca
      901 aaccgtatat agaataaaaa aagacagcat ggtcaatcac gaatagttga tcatgctgct
      961 gtttctattt tatttgctta ctttttgaaa aactgttcta actttcatca ataactcctc
     1021 aactaaacta tcatgcgctt tttccaaaaa aatacactct ctggcttcat aatcaattgt
     1081 cgttagttga tctaataata cttttcctgt tgtcttcatt tcatattctg agtttatatt
     1141 tacatatagc ggatagtttc tagttgtact agaaattgga gcaactgtaa caacgttact
     1201 ataatccgca ataatatcat gacttaaaac aatataaggt cttttacctg tctgttcgtg
     1261 tccagatcga ggtgctgtat tcaataatag aatatcgcct tgtctaggtt ttctcaccat
     1321 ttttcattac ctactgcttc acctaaattt tgaatcgtca catgatttct ttcatcagtg
     1381 taattttcaa ataactgttc aattgttaat tcattttcta tattagccgc ttcgataatt
     1441 agccgcttct gaccgtttac ttcttcttgt attatcttca ctttgtcttc tgtatgaatt
     1501 ccaagatatt ccataatata ctttggcaat ctaaggccat tactatttcc ccattttttt
     1561 ataggtaaca tttccatagt agacacctct ttctgtatat acatagtata acatacataa
     1621 gaaaaaacgt atatacttaa ttatcctttt ctattaaatt agattaacat tccatgaatc
     1681 cataaactat gttaacattt atatgaaaat agtcgaaagg aaatcataat atgtttaaga
     1741 aaacaaaatc tttagaatca ggtaagaaaa aaattgatct aaaaataatc tcttctggat
     1801 actttaaaga tttcatagtt attgatacaa aatggaataa aaaggacata gaaaat
//
LOCUS       DACDNO010000162         1787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDNO010000162 DACDNO010000000
VERSION     DACDNO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.170"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..346)
                     /locus_tag="IWI22_002886"
     CDS             complement(<1..346)
                     /locus_tag="IWI22_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9170193.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIH"
     gene            complement(351..536)
                     /locus_tag="IWI22_002887"
     CDS             complement(351..536)
                     /locus_tag="IWI22_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9170194.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(890..1135)
                     /locus_tag="IWI22_002888"
     CDS             complement(890..1135)
                     /locus_tag="IWI22_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9170195.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(1129..1530)
                     /locus_tag="IWI22_002889"
     CDS             complement(1129..1530)
                     /locus_tag="IWI22_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9170196.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT          575 a          322 c          230 g          660 t
ORIGIN      
        1 tatgaattgg tcgaatcttt acaacctgca tttctccaaa aaaatccaat gcacgttgta
       61 aaatatgatc tgttttcata tatgtttttt ctacagttcc caacttatac caaggaaaaa
      121 aatactctcg ataaaaatca tgaaacgtta ttccttgcag ataaattcct tgtgaatcag
      181 aaaaactttg ttttaactct tcgagtcccc ttctagcttc tccttgagtt ttgaagcctc
      241 ttctggtcac ttgaattctt tttccaaagc gatcttgtcc taaagttgca gaaaaaaacc
      301 aagtgcctct ttttctgtct ttataaacat tcgcaatttt cctcatttta ctattcctcc
      361 caagaaatat ggagaacttc ttctaaaatt gaaataggaa ttctttttat ttttgaatta
      421 cgataaaaaa agtatccttt ctcaactaaa agttctttac attccttgaa gattttatta
      481 gcatagtact gagaaaaacc tagattaatc aaatcttttc gagtagctgt tttcattata
      541 gcaccttcct tacttttttt catttatttc tatcagctgc ttttgtcaaa agcacatagg
      601 aatttcacct ctccccaagt ttacggcgag ccttacggaa gtatcattat cccaacttta
      661 aatcatcgct ttttattgac tataaaaatt attttagaat atttatcgct ccttctttac
      721 agcaagaaat catggcgcaa ctaaaatatg ctccttaaag tgttaacgta ctgatagaat
      781 ctatttaatt ttcaatgaac aataggtttt ctacctaaaa gaatctacta attccttaac
      841 tattagaaga ttcttttaaa cagaaaaagt ggaaagggga tttttttaat tagttaattt
      901 taaattttaa gatcgcttct attaatttaa tttctagctg acttatcata tattcatcta
      961 cataggtaac gaattgacct acctcgttaa atccatttgt caaacataac ttagccatat
     1021 aagaatgata atgtttgagt atcatcttga tcgaaattgg atctccttct agagcagata
     1081 agataatgga tagtggaact aataggtact gccgcttttt agatttatct accataggtc
     1141 ataccccatt ttcaattgtt caactgcctt ctttcgttga taccagatac cacttgtgct
     1201 catattgaga attttaccga tttcagcatc attaaaacct gcaaaataat aaagtaggat
     1261 aatctttctt cttatctccg ataactgatc aattgctgct gataactttt gatctgaaat
     1321 taatatttcc attccaaata agaaatagac atcttgttca tcaaaatgag tttgggaata
     1381 cctgccttca tttgaatctg ccaatggttc ctcattatcc tttatgttag cgtttcgttt
     1441 tcgaatagac ttagcttcgt ttcgtaatac ttttttacag taggcatcaa attggtgttg
     1501 tatagatatg tcattgtaat gttgttgcat gataatatgc tcctttttcc ccctttccac
     1561 taatacaaca atcagaaagc cccttttttt cgaaaaaact agaaaaaaaa cataaaaata
     1621 tttatcattc tttaaacaga aaatctttct ataaaaaaca aaaaaaacga ttaggctagg
     1681 ctaatcgttt tttgaacatt cttaaaaaat ccaaattatt ttttataact gaaattgggt
     1741 attgatgacc atcgtttctg tagatagtaa gcaatcattt taggttt
//
LOCUS       DACDNO010000163         1776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDNO010000163 DACDNO010000000
VERSION     DACDNO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.171"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            131..520
                     /locus_tag="IWI22_002890"
     CDS             131..520
                     /locus_tag="IWI22_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP9170197.1"
                     /translation="MGDIDLENIFDLNKGLSRRKYWYYQCILFVFGLGIETFSMFVYK
                     KSLIENNYLKYFVINGPFDFYNFLRIVFFIMIIIGFLFQARRFVDIGWSKFVALLSLL
                     PLVGIITTGICLFKKGKDNKTDSSQVS"
     gene            1019..1678
                     /locus_tag="IWI22_002891"
     CDS             1019..1678
                     /locus_tag="IWI22_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfX family protein"
                     /protein_id="HAP9170198.1"
                     /translation="MEENNIESKDNLFKEIGEKMYPLFFNRNFGLATKSEIESMLFYS
                     YLKHKINSNSDYSDRTLSRELGISEVKVRNLKRTCYARYEDAIDFPKLLTSLSKEGSN
                     LMFFKIYTDNNDVLCRISISEVVYYDELRTQLNNSGILFKKNPGSNYVELSMADAIIF
                     FDQDSISKESLDLKNLIDKMKQLNFKTNTKMDIDKILEGTKAGRMKNVVEAMMKLSNS
                     I"
BASE COUNT          633 a          196 c          317 g          630 t
ORIGIN      
        1 taaattcaat cagaaatgta tactttgtaa ggaaaaaatc atagctgttt taggatatta
       61 tttgctattt tatgtatttg ccaattacaa tggtattgta aataaataca taaagtagca
      121 ttagtaggtt ttgggggata tagatttgga aaatattttt gatttaaaca agggattatc
      181 aagaagaaaa tattggtatt atcagtgtat actgtttgtg tttggattag ggatagaaac
      241 tttttcaatg tttgtataca aaaagtctct gattgaaaac aattatttaa aatatttcgt
      301 gataaatggt ccatttgatt tttataattt tcttaggatc gtgttcttta taatgattat
      361 tataggtttt ctgttccaag caagaaggtt tgttgatatt gggtggtcga aatttgtagc
      421 tttactaagt ctgttaccgt tagttggtat tatcacaaca gggatttgtt tatttaaaaa
      481 gggaaaagac aataaaacag atagcagtca agtttcttga ttgctatctg ttttatttat
      541 taagagtatt gtctattaaa tgaatgacaa gaacttgcat tgtaagcagc ttgaatcgca
      601 gctgaccatg agagtttatg gaaggctact gcagaccaaa ttcctttcat aatacaagca
      661 ccttgttttg ctttagcttg ttgatattta ttccataaat gtttagggac tttacttttt
      721 gcaaagttgt cctaataacc atcgtcactt tgggcatatg cagtagtaaa gtttactaac
      781 ggataataca tgataaattt aaaaggtagg gtactttttc gtacaagaat gattttgtaa
      841 gtatgaaaca agctagttat atttcactag ctattcaatg aaataagttt ttaaagtaga
      901 ttgttgatag atatattaaa attttttttc atgtaagagt atgctaaaat tgatgttaag
      961 aatggttttc cctagtaata ggtaacatca aaataaagga aggtgtaatt ctatacctat
     1021 ggaagaaaat aatatagaaa gcaaagacaa tctgtttaaa gagattgggg aaaaaatgta
     1081 tccgcttttt tttaatcgta attttggatt agcaacaaaa agtgaaattg aaagcatgct
     1141 cttttactcg tatctaaaac ataagataaa tagtaatagt gattactcag atagaacctt
     1201 gtctagagag ctaggaatat cggaagttaa agtaaggaat cttaagagaa catgttatgc
     1261 aagatatgaa gatgcaattg attttcctaa attactaacc tcgctgtcca aagaaggctc
     1321 aaatctaatg ttttttaaaa tctatactga caataatgat gtattatgta ggatatctat
     1381 ttccgaggta gtttattatg atgaacttcg aacacaatta aacaatagtg gtattctttt
     1441 taaaaaaaat ccaggatcaa attatgtaga gttatcaatg gcagatgcaa taattttctt
     1501 tgatcaggat tctatcagca aagaaagctt agatttgaaa aatttaatag ataagatgaa
     1561 acagttgaat tttaaaacta atactaaaat ggatattgat aaaatattag aaggaactaa
     1621 agctggtaga atgaaaaatg tcgttgaggc tatgatgaag ctttccaact caatataaac
     1681 aaagaggtca gaaacttcct aagttttttg acctattgct ttttcgtgtg taactttttt
     1741 atttaaatgg cataagtttt tatcgatgaa tggcaa
//
LOCUS       DACDNO010000164         1770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDNO010000164 DACDNO010000000
VERSION     DACDNO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.172"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            197..424
                     /locus_tag="IWI22_002892"
     CDS             197..424
                     /locus_tag="IWI22_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9170199.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            547..873
                     /locus_tag="IWI22_002893"
     CDS             547..873
                     /locus_tag="IWI22_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9170200.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            941..1654
                     /locus_tag="IWI22_002894"
     CDS             941..1654
                     /locus_tag="IWI22_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9170201.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          686 a          273 c          373 g          438 t
ORIGIN      
        1 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
       61 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
      121 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
      181 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
      241 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
      301 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
      361 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
      421 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
      481 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
      541 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
      601 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
      661 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
      721 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
      781 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
      841 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
      901 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
      961 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
     1021 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
     1081 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
     1141 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
     1201 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
     1261 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
     1321 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
     1381 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
     1441 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
     1501 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
     1561 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
     1621 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
     1681 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
     1741 aacgcaaaga ggtgcatctc tgatttggtt
//
LOCUS       DACDNO010000165         1739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDNO010000165 DACDNO010000000
VERSION     DACDNO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.173"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..238
                     /locus_tag="IWI22_002895"
                     /pseudo
     CDS             1..238
                     /locus_tag="IWI22_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
     gene            222..614
                     /locus_tag="IWI22_002896"
     CDS             222..614
                     /locus_tag="IWI22_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9170202.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            615..>1739
                     /gene="aac(6')-Ie"
                     /locus_tag="IWI22_002897"
     CDS             615..>1739
                     /gene="aac(6')-Ie"
                     /locus_tag="IWI22_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP9170203.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDF"
BASE COUNT          715 a          133 c          298 g          593 t
ORIGIN      
        1 ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg attttggaga
       61 ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata gtgaagaaga
      121 aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag atattgagaa
      181 agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta ttgtttattg
      241 atgataatat ggtttttgat attgataatt taaaaggatt tcttaatgat accagttcat
      301 ttgggtttat agctaaagaa aataataaaa ttataggatt tgcatattgc tatacacttt
      361 taagacctga tggaaaaaca atgttttatt tacactcaat aggaatgtta cctaactatc
      421 aagacaaagg ttatggttca aaattattat cttttattaa ggaatattct aaagagattg
      481 gttgttctga aatgttttta ataactgata aaggtaatcc tagagcttgc catgtatatg
      541 aaaaattagg tggtaaaaat gattataaag atgaaatagt atatgtatat gattatgaaa
      601 aaggtgataa ataaatgaat atagttgaaa atgaaatatg tataagaact ttaatagatg
      661 atgattttcc tttgatgtta aaatggttaa ctgatgaaag agtattagaa ttttatggtg
      721 gtagagataa aaaatataca ttagaatcat taaaaaaaca ttatacagag ccttgggaag
      781 atgaagtttt tagagtaatt attgaatata acaatgttcc tattggatat ggacaaatat
      841 ataaaatgta tgatgagtta tatactgatt atcattatcc aaaaactgat gagatagtct
      901 atggtatgga tcaatttata ggagagccaa attattggag taaaggaatt ggtacaagat
      961 atattaaatt gatttttgaa tttttgaaaa aagaaagaaa tgctaatgca gttattttag
     1021 accctcataa aaataatcca agagcaataa gggcatacca aaaatctggt tttagaatta
     1081 ttgaagattt gccagaacat gaattacacg agggcaaaaa agaagattgt tatttaatgg
     1141 aatatagata tgatgataat gccacaaatg ttaaggcaat gaaatattta attgagcatt
     1201 actttgataa tttcaaagta gatagtattg aaataatcgg tagtggttat gatagtgtgg
     1261 catatttagt taataatgaa tacattttta aaacaaaatt tagtactaat aagaaaaaag
     1321 gttatgcaaa agaaaaagca atatataatt ttttaaatac aaatttagaa actaatgtaa
     1381 aaattcctaa tattgaatat tcgtatatta gtgatgaatt atctatacta ggttataaag
     1441 aaattaaagg aactttttta acaccagaaa tttattctac tatgtcagaa gaagaacaaa
     1501 atttgttaaa acgagatatt gccagttttt taagacaaat gcacggttta gattatacag
     1561 atattagtga atgtactatt gataataaac aaaatgtatt agaagagtat atattgttgc
     1621 gtgaaactat ttataatgat ttaactgata tagaaaaaga ttatatagaa agttttatgg
     1681 aaagactaaa tgcaacaaca gtttttgagg gtaaaaagtg tttatgccat aatgatttt
//
LOCUS       DACDNO010000166         1652 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDNO010000166 DACDNO010000000
VERSION     DACDNO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.175"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            96..506
                     /locus_tag="IWI22_002898"
     CDS             96..506
                     /locus_tag="IWI22_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9170204.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            520..876
                     /locus_tag="IWI22_002899"
     CDS             520..876
                     /locus_tag="IWI22_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9170205.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(1484..1627)
                     /locus_tag="IWI22_002900"
     CDS             complement(1484..1627)
                     /locus_tag="IWI22_002900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170206.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            1546..>1652
                     /locus_tag="IWI22_002901"
     rRNA            1546..>1652
                     /locus_tag="IWI22_002901"
                     /product="16S ribosomal RNA"
BASE COUNT          591 a          256 c          311 g          494 t
ORIGIN      
        1 ttattttgat ttttttttat attttattag ctaataattg gtattttaaa agaaattact
       61 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
      121 gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag
      181 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagacc ttggcagcga
      241 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      301 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
      361 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      421 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      481 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      541 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      601 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
      661 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      721 attgcgattg acctttcctg tgtagaaaaa aaacaactga tttttttatg cacacaacat
      781 accaacctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      841 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      901 ctgagcattc catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
      961 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
     1021 tctaggtggg gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc
     1081 atttctgttg ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag
     1141 ttgttgacat gttagaaatg acatgataag ataactgagt cgttaaaaaa ggaccgcttg
     1201 cgaagcttga ttctatcgaa aaaacttcaa aaaagttctt gacaaataac aaacgacaag
     1261 ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa
     1321 gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctgcggca gccaaaaacg
     1381 aatcgggaaa cactcctgat gaagtagacc tttgaaaaat gaacaaagta agacaaacca
     1441 aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca tttttaacaa gcaagcaata
     1501 tgctagcaaa caaattgagc ttaacaatcg caaggttgtt cgaacttttt atgagagttt
     1561 gatcctggct caggacgaac gctggcggcg tgcctaatac atgcaagtcg aacgcttctt
     1621 tttccaccgg agcttgctcc accggaaaaa ga
//
LOCUS       DACDNO010000167         1561 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDNO010000167 DACDNO010000000
VERSION     DACDNO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.176"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="IWI22_002902"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="IWI22_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAP9170207.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            153..887
                     /locus_tag="IWI22_002903"
     CDS             153..887
                     /locus_tag="IWI22_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAP9170208.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          583 a          247 c          211 g          520 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aacctcatag gagtgggtta taatgaacaa aaaaaatata aaagatagtc
      181 aaaactttat tacttcaaag catcatataa atgaaatttt gagaaatgta catttaaata
      241 caaatgataa tattattgag attggttcag ggaaaggtca tttcacgttt gaattagcta
      301 aaaggtgtaa ttatgtaacc gccattgaaa tagatcctaa attatgtagg ataactaaaa
      361 acaaacttat tgaatatgag aacttccagg ttatcaataa agatatttta caatttaagt
      421 ttcctaaaaa taagtcatat aagatatatg gaaatatacc ctacaatata agtacagata
      481 taattcgaaa aattgttttt gaaagcacag ctacagaaag ttatttaata gtggaatatg
      541 gatttgctaa aaggttgcta aatacaaatc gttcactagc actattttta atgacagaag
      601 ttgatatatc catattaagt aaaatcccta gagaatactt tcatccaaaa cctagagtta
      661 atagctcgtt aattgtatta aaaagacacc cttcaaaaat atcactcaaa gatagaaaac
      721 aatatgaaaa ttttgttatg aaatgggtta acaaagaata cataaaacta ttttccaaaa
      781 accaatttta tcaagcctta aaatatgcaa gaattgacga tttaaacaat attagctttg
      841 aacaattctt gtctcttttc aatagctata aattatttaa tagataagtt aaggggtgca
      901 taaactgcat cccttaactt atcttatttt gcttaaacaa tcttggcccg taaactgcat
      961 aactcatatg taaatcgctc ctttttaggt ggcacaaatg tgaggcattt tcactatttc
     1021 cggcaattac ttccatgtaa agtataaccc actatacttt gcaagcaaag tgtgggctcg
     1081 ccgaggggtc ttgcagacgg ctctttcggc tcttttgagc cgagtatcag cgcaccctta
     1141 tggtgcaatt agctgatacc tgtttgacga tttgctgccc taccttttcg gtaggtatgc
     1201 aaacctcaaa ccaaaataaa aacccccttc gacttcgtca gggggttttt atttatataa
     1261 atctccgtaa ataatcgcga tttaataaca tcttcaattt tatttttttg cgtattttag
     1321 aaatgttttc actaaaaaat atattataca aaaaatacag taatcgatta taattctttg
     1381 ccagttaaat atcatatcgt tattaaaata tcggaaaaaa atatcaacaa caataaatgc
     1441 aaaagaagac cataaaaatt gtttattact catacatact ctccatttct atcattttta
     1501 aatttaccag tcatctctca aaaatctcct agctattttt cttttttaac cacataccgt
     1561 c
//
LOCUS       DACDNO010000168         1463 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDNO010000168 DACDNO010000000
VERSION     DACDNO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.178"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            83..1423
                     /locus_tag="IWI22_002904"
     CDS             83..1423
                     /locus_tag="IWI22_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9170209.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          462 a          323 c          284 g          394 t
ORIGIN      
        1 cttttttcta ataaacgcaa acagacacct tgaaatcctt tagaataaag tcgacgaaaa
       61 ccattcaaag gagtgtttca agatgtccta ttcagagttt accaaagagt tactagatat
      121 tctagaccta aatcttacct ttcatgaaga tgcctttcgc aaagagcgca ttaatgacga
      181 aacatgtttt gtttttgatg gcacattgac ctatcaacca gaggaatgct tccattgcca
      241 ctatcaaaat aagcaaacga tcattaaatg gggctggaaa aaagtatcca tcttattgaa
      301 tgatgtcagt aactacaaga ccattgtgcg gattaacaaa cagcgcttca aatgtaaaca
      361 ctgtggaaag acttttttag cagaagattc agttagcgat cgtcgctgct ccattgcccg
      421 cagagtcaaa caagccattc ttgagctatt aagtgagcct atttctatgt ctttgattgc
      481 acgtatgaag cacatttctc cgaccactgt cattcgaata cttcgcagcc ttcggcctaa
      541 aaccgtttct ttgaaccagc cgttaccaga agtggtgtgt ttcgatgaat tcaaatcggt
      601 gaaaaacgtc tcgggagcca tgagctttgt catgatggac ggcaagaccc atcaactgat
      661 tgatattgtg gaaaatcggc aactcaatcc tttacgcgac tatttcttgc gctaccctag
      721 aaaagtccgt gaacaagtac gtctagtggt ttcagatttc tatacaccct atcgcacatt
      781 ggtgaaagag tgttttccga atgcccgaat cgtcgccgat cgctttcata ttagccaaca
      841 catcggtcgg gcctttacca accaccgaat tcaagtaatg aagaccttta aaaaagggga
      901 tcgtcggtcg aagcacttaa agaaatactg gagactactt caaaagaatg cgtgggaatt
      961 gaatggtcag catcgctact ggcgtccgtc atttcgagat catctgacag aagcggagat
     1021 agtcgatcgg ctattatatt atgatgactc cttaaagcga ggctacgaag tctaccaggt
     1081 ttttctttcc gctatcagaa gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga
     1141 ctacaaggag ttgccagagc actatcaacc agtgttcacg accttcaaga aatatcgaac
     1201 agaaatcaaa cgagcgttgc gagttcccta ttccaacggc cctatagaat gtttgaacaa
     1261 ccatatcaaa gtactaaaac gaattgccta tggctttaga aactttcaaa actaccgaga
     1321 acgaatcttt ttatatcgag gaaaatattt caagaaaact aagaacacta cccaactgac
     1381 taaagccaga acaacaacac ggctagacaa gatagctgtc tagccgtgtg agtatctcga
     1441 ttattctatt gaatttcttc acc
//
LOCUS       DACDNO010000169         1455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDNO010000169 DACDNO010000000
VERSION     DACDNO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.179"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            108..1040
                     /locus_tag="IWI22_002905"
     CDS             108..1040
                     /locus_tag="IWI22_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP9170210.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1455
                     /locus_tag="IWI22_002906"
     CDS             1112..>1455
                     /locus_tag="IWI22_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170211.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          227 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caaga
//
LOCUS       DACDNO010000170         1419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDNO010000170 DACDNO010000000
VERSION     DACDNO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.180"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            86..1381
                     /locus_tag="IWI22_002907"
     CDS             86..1381
                     /locus_tag="IWI22_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9170212.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          447 a          279 c          267 g          426 t
ORIGIN      
        1 atcttttttg tataaaaaaa tatagaaaag acaccctgaa atcctttaga attaatttgg
       61 ctaccaattc ataggaggat tcaagatgtc tcatactcag cttatcaaag ataccctaaa
      121 tattctagac ctaaatatct atttcgagga aaattgttta actaaagaaa aatacaaagg
      181 gcaaatttgc ttgatttaca aaggaactct tctgtacaag ccagaagagt gttcccattg
      241 tctttgtgtc gtcccctcta ggatcattcg ttgggggacg acaaccgttc ggttgttact
      301 gaatgatgtg tctgagtatc gcacttatct ggagctgaag aaacaacgat ttaaatgtaa
      361 gtcttgccaa agaacattcg tagcggatac ctctgttgct gaaaaacact gtttcattag
      421 tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag aatacttcca tgacggaaat
      481 cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt gtcatgaagc gattctaccg
      541 cccattgaat cctttcaaac agactttgcc aaaggtctta tgctttgatg aattcaaatc
      601 ggttcgaagt gttgccagtg caatgagttt tatcatgatg gacggacaaa gccatgcatt
      661 attggatatt gtcgaaaatc gacgcttgcc ttatttagaa cgctattttt ctcggttttc
      721 attagcgact cgtgaagccg ttcaattgat tgttattgat atgtatgcgc cttatgtttc
      781 gttggtgaaa aagcttttcc cgaatgctca gttgatcatc gatcgtttcc acatcgtcca
      841 acatattggc agaactttct tgaatcatcg agtgaaagaa accacggtac gacttaaagg
      901 aaattcccac tctcaacgag ggttagggaa aaaattgaag cgctattgga aactcctaca
      961 aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt ttaaagatta
     1021 tttgactgaa agtgagattg ccgatcgact gttagctgct tgtcccaact tacgccaagg
     1081 ctatcagtta taccaagaca ttctttatgc ggtaaagaaa agagatcagt cactatttga
     1141 ggattgttta actagagaca ttagcggtct gccagaaacc tatacaacca ctttacgaac
     1201 gtttaaaaag tttctgccac agatccaaaa tgcgcttcac tacagctatt caaacggacc
     1261 attggaatgc ttaaacaacc acatcaaagt attaaaacgc aatgcgtacg gctttcgcaa
     1321 cttttacaat ttcaaactac gaattatgat tcgtcatggc aaaacatttt taaccaaata
     1381 aacagaacag aaagaggttt tcttctttct gttctgtcc
//
LOCUS       DACDNO010000171         1384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDNO010000171 DACDNO010000000
VERSION     DACDNO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.183"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..1046)
                     /locus_tag="IWI22_002908"
     CDS             complement(27..1046)
                     /locus_tag="IWI22_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9170213.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1057..1263)
                     /locus_tag="IWI22_002909"
     CDS             complement(1057..1263)
                     /locus_tag="IWI22_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9170214.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          380 a          298 c          231 g          475 t
ORIGIN      
        1 taatgctcct caacgaggag ctatttttat cgtttaggaa tatttaaata ccaacgtttg
       61 tcatggaaat cttgcgcacc gcctttagtg tttccctctg gatcattcgt tgcccgcatc
      121 atgacataga ctttcttatt agggaagtta cgcatgttaa aagatacatg atagccaaca
      181 tttccagaag tattataagc ttgatttaca tctgatctat aaattccatc agctcttact
      241 cgagctaatt ctttcccagt attgtaatcc ataatgaaga tatactcgta tttatagtta
      301 gcaatgtgcc atccagctac atgcaaattt gcattttcga tttctccaaa ctgatcaatg
      361 tgggcgtaat tcgttccatc tgtcagtgta ggattagctg caccagctcg tgttggatca
      421 atgacaggct tgttttcaga agttgttgga ttttcatcgg taaatccatg agctaaatca
      481 tatgctaatt tttctttact tacgcccatt tcagaaagat aaccgtaagg atctgtatga
      541 tcgccccaga tgttttgtgt tacccataaa tgcgatttga ttcccggttg gttataagga
      601 gtgtccaacg ttaatggaat accatatttc attgctgaat ctctagccaa ttcaacgtat
      661 gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt gttgtaactc aatctgcaca
      721 ggactgttgg cattagcata cgaaccagca ccgtactgta cataaccagg ttgaccgact
      781 tgataaacaa ttccgccgtc tcccacaata taagcagtat aagcgctagt ccatgaacgt
      841 tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat tagccgtttc atgcagtaaa
      901 atatactgat tatttgctac ttgtgagcta ccttcatttg cgcccaaatt aaattcattg
      961 ttgatagtat aggcaaatcc gttaattggc aataaaaaaa gagccgttaa caggctcatc
     1021 gcagtaatag taattttctt tttcatatct atcctcctat ttatctgatg aaattttctt
     1081 atacgttcga tttgatacac ctaaaatact ccctagaaat gctccaaaac ctgtaataat
     1141 catcaccgtt atatctgtgt accgccagtt aacacctttt ccgacaagac cgacaaaagt
     1201 cgctaaagcg gggataacta ctatagcgat ccatttcaaa atatcaaagg ttctattgtt
     1261 catactatcc acctcccttc attggttggg ctactttctt tttcaatcgc ttgttttctt
     1321 ctatcaatat ttctctttct gaaagcaagg catcaatttg cccgtccagt tgttcaattt
     1381 tatc
//
LOCUS       DACDNO010000172         1369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDNO010000172 DACDNO010000000
VERSION     DACDNO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.184"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            39..1340
                     /locus_tag="IWI22_002910"
     CDS             39..1340
                     /locus_tag="IWI22_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9170215.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          447 a          274 c          253 g          395 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
     1261 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
     1321 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgtt
//
LOCUS       DACDNO010000173         1356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDNO010000173 DACDNO010000000
VERSION     DACDNO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.185"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>1356
                     /locus_tag="IWI22_002911"
     CDS             82..>1356
                     /locus_tag="IWI22_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9170216.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLIN"
BASE COUNT          461 a          261 c          257 g          377 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaat
//
LOCUS       DACDNO010000174         1356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDNO010000174 DACDNO010000000
VERSION     DACDNO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.186"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1343
                     /locus_tag="IWI22_002912"
     rRNA            <1..1343
                     /locus_tag="IWI22_002912"
                     /product="23S ribosomal RNA"
BASE COUNT          369 a          295 c          409 g          283 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaa
//
LOCUS       DACDNO010000175         1341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDNO010000175 DACDNO010000000
VERSION     DACDNO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.187"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(742..1083)
                     /locus_tag="IWI22_002913"
     CDS             complement(742..1083)
                     /locus_tag="IWI22_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9170217.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1083..1313)
                     /locus_tag="IWI22_002914"
     CDS             complement(1083..1313)
                     /locus_tag="IWI22_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9170218.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          411 a          224 c          197 g          509 t
ORIGIN      
        1 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
       61 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      121 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      181 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      241 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      301 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      361 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      421 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      481 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      541 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      601 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      661 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      721 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      781 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      841 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
      901 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
      961 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1021 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1081 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1141 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1201 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1261 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1321 actcccttaa taaaagtata c
//
LOCUS       DACDNO010000176         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDNO010000176 DACDNO010000000
VERSION     DACDNO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.188"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(190..801)
                     /locus_tag="IWI22_002915"
     CDS             complement(190..801)
                     /locus_tag="IWI22_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9170219.1"
                     /translation="MGIQIISLSDLLEVAPDLESVNSILKTFKSIPHPITGQVNDVEY
                     FLHQKAIEFEKAALATTHLLFSSYKDKSILVGYFSLANKSLIMSKKNYNNLSKPQQRR
                     LCQNGSKTETGGYIVNSYLIGQVGKNYSDEAQKIDAINGTQILTIAYDTVLQAKKIIN
                     ARYVWIECENIPRLIDFYRDFGFEVIENYETNSGLVVMVMKLK"
BASE COUNT          450 a          228 c          154 g          482 t
ORIGIN      
        1 taaatacttc atatcaatca tttattcaga aacaaactat cttgcaggat ctatacgagt
       61 taattacaga gcaaaaaagc ctcaaccaag gatgactggt tgaggttttt gttttacaaa
      121 tttaaatctt tttaagttca ctatacgctt gtttaacctt gtaatagata catgcaataa
      181 ttagttttac tattttaact tcataaccat tacaactaat ccactgtttg tttcataatt
      241 ttcaataacc tcaaaaccaa agtctctata gaaatcaatt aacctcggaa tattttcaca
      301 ttcgatccat acatatctag cattaataat tttttttgcc tgcaaaaccg tatcataagc
      361 tatcgtcaat atctgggttc catttatagc atctatcttt tgagcttcat cagaatagtt
      421 tttgccaact tgtcctatca aataactatt aactatgtaa ccaccagttt cagtttttga
      481 accattctga cacaatcttc tttgttgggg cttactgaga ttgttataat tttttttgct
      541 cataattaag gatttgttag ctaaagaaaa ataaccaact aatatagatt tatctttata
      601 tgagctaaat aaaagatgcg tagttgctaa agcggctttc tcaaattcta ttgctttttg
      661 atgcaaaaaa tattccacat cattaacttg accagttata ggatgtggaa tagatttaaa
      721 tgtctttagt atgctattta cactttctaa atccggagca acctctaata aatcacttaa
      781 actgataatc tgtattccca tctaactatt acttccaaaa aaaatatttt ttattttttc
      841 tttgtcatcg attactttta aattagttac caactgtttt ctaggtgatt tatcattaga
      901 taatgcatta attaaagagt tagcattctt ttttgtaaac ctatactcct cagtaaaact
      961 tttagtagcc ataacaatcg cctccgttta tgtaccaaca caaaaatata taggaaaaaa
     1021 cacgtcaaaa taacgtactt gtacaaaaca atcatacatc attttacgct ttaactttcc
     1081 tttgttgtaa gttcaatata ccatcattgg tttaattgta caagagaaac ggctaaaaaa
     1141 tcagctaatt ttgttagcaa ctttgttagc atatctgaca gtagatcaat agtaaaagtg
     1201 cctgtatata aggaaaaact gtatttttac tatctaaatt cgtaaacatt tttgcttaca
     1261 aattagctaa taatattagc aactatattg aaaactatat gtactgcccc tcac
//
LOCUS       DACDNO010000177         1192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDNO010000177 DACDNO010000000
VERSION     DACDNO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.189"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWI22_002916"
     CDS             <1..1076
                     /locus_tag="IWI22_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9170220.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DACDNO010000178         1175 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDNO010000178 DACDNO010000000
VERSION     DACDNO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.190"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1175
                     /locus_tag="IWI22_002917"
     rRNA            <1..>1175
                     /locus_tag="IWI22_002917"
                     /product="23S ribosomal RNA"
BASE COUNT          351 a          235 c          348 g          241 t
ORIGIN      
        1 ctgaaacatc taagtacctg caggaagaga aagaaaattc gattccctga gtagcggcga
       61 gcgaaacggg aaaagcccaa accagcaagc ttgcttgttg gggttgtagg actccaatat
      121 ggtagttctt tcagatagtc gaatgacttg gaaaagtcag tcaaagaggg taaaaacccc
      181 gtagacgaaa tgtggaagac acctaggagg atcctgagta cggcggaaca cgagaaattc
      241 cgtcggaatc cgggaggacc atctcccaag gctaaatact ccctagtgac cgatagtgaa
      301 ccagtaccgt gagggaaagg tgaaaagcac cccggaaggg gagtgaaata gaacctgaaa
      361 ccgtgtgcct acaacaagtc aaagcccgtt aatgggtgat ggcgtgcctt ttgtagaatg
      421 aaccggcgag ttacgattgc atgcgaggtt aagttgaaga gacggagccg cagcgaaagc
      481 gagtctgaat agggcgtttg agtatgtagt cgtagacccg aaaccatgtg atctacccat
      541 gtccaggttg aaggtgcggt aaaacgcact ggaggaccga acccacgtac gttgaaaagt
      601 gcggggatga ggtgtgggta gcggagaaat tccaaacgaa cttggagata gctggttctc
      661 tccgaaatag ctttagggct agcctcggaa ttgagaatga tggaggtaga gcactgtttg
      721 gactaggggc ccatctcggg ttaccgaatt cagataaact ccgaatgcca ttcattcata
      781 tccgggagtc agactgtgag tgataagatc catagtcgaa agggaaacag cccagaccac
      841 cagctaaggt cccaaaatat atgttaagtg gaaaaggatg tggggttgca cagacaacta
      901 ggatgttggc ttagaagcag ccaccattta aagagtgcgt aatagctcac tagtcgagtg
      961 accctgcgcc gaaaatgtac cggggctaaa catattaccg aagctgtgga gtacaccttt
     1021 aggtgtattg gtaggagagc gttctaaggg cgttgaaggc agatcgtgag gactgctgga
     1081 gcgcttagaa gtgagaatgc cggtatgagt agcgaaagac aggtgagaat cctgtccacc
     1141 gaatgactaa ggtttcctgg ggaaggctcg tccgc
//
LOCUS       DACDNO010000179         1159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDNO010000179 DACDNO010000000
VERSION     DACDNO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.191"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            26..>1159
                     /locus_tag="IWI22_002918"
     CDS             26..>1159
                     /locus_tag="IWI22_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HAP9170221.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TDYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRHVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSLYAKRGT
                     MENFIKEAKAGVYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRT"
BASE COUNT          368 a          243 c          213 g          335 t
ORIGIN      
        1 caaccaaaag aaaggagttc ttttcatgac tagcttacac aaaaaccaag taaaattcaa
       61 ttcaaatatc actatttctc atacaggtgg tcaattatcg agtgattcgg gtctcgtcct
      121 agtgaaagag ctaatgaaca tctttggttt ttctgaactg gctaagcaac acattcacat
      181 tgaagacgaa cgagcgtatt ttactcatga caacttatcc atacttgagc agttcattat
      241 gcaattaatt gctggttact cagctgattc tgcagctaat ctcctgagac aagatcctgt
      301 gtttaaagct gttttagata ggaaagaact cgcttctcaa tcttcacttt cccgattttt
      361 agatcgacta tctgaggaga atatccatga acttcaagct ttgaaccaag aacttatcga
      421 taaagcacgc ctcatccgta atgatacgga attaatcatt gatttagatt cgacccattc
      481 agatacattt ggtcaccaag aacaaacgga ttataataca cactaccaaa cctatggtta
      541 ccatccattg gtagcttttg acggattgac tggtgacttc ttaaaagctg aactacgttc
      601 aggaaatcaa tatacatcaa aaggcgtaaa ggagtttctc acacctttat tagagcacta
      661 taatcactct ctaccaaaca ctgacatctt ggttcgtgga gacagcgggt tcgctacacc
      721 tggtgtgtat gattcgtgtg aatcaaaaaa gagtcattat gttattcgac tgaagaataa
      781 tcgtagacta ggtcaaatcg ctgagaaatc agtactttat ggcgataatc aaaagtggga
      841 agaacgagaa gttcagtact tctcgaccac ttatcaagca caatcctggt cacaaagtcg
      901 tcacgtgtgt atacgctcaa cacgtgaagc gggcgaacta ctctttcgac atgaatttat
      961 cgtgacgaat ctatcagaaa atgtttctcc tgataccatc ttttctctct atgccaaacg
     1021 tggcacaatg gagaacttca ttaaagaagc gaaagctggc gtttactttg acaaaacaga
     1081 cagtcctcgc tttttggaga atcatgtccg aatgatgctc agcttgatag cttacaactt
     1141 agtcaacttc ttaagaacg
//
LOCUS       DACDNO010000180         1126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDNO010000180 DACDNO010000000
VERSION     DACDNO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.192"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(208..606)
                     /locus_tag="IWI22_002919"
     CDS             complement(208..606)
                     /locus_tag="IWI22_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9170222.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(603..839)
                     /locus_tag="IWI22_002920"
     CDS             complement(603..839)
                     /locus_tag="IWI22_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9170223.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          361 a          198 c          153 g          414 t
ORIGIN      
        1 aaatatataa aaaatcagcc cactagttct aaaccactat ttcctactat tcactctttt
       61 gtattccttt taaatcaatt atttcaaaat taatccatat tttccatatt tttatgaaaa
      121 aactattttg tatgattaaa cataaaaaaa tcagcatatt ccttaatgac atgctgatct
      181 aattgagaaa taaagttttt aattcgttca ttttattgcg gtttgtcgta aataactggt
      241 tatcttggac tttagtgaat cgaaatccaa tgaactagtg gtaatcatta tgataaaatc
      301 aacagcttct tgacgagaaa aagcaactgg acaattattc aattgaagaa acaacaacat
      361 ggcaacaaga gcagttcgtt tattgccatt atgaaaagga tgttttttag ctaaattaat
      421 tgctaaaatc gccgcttttt catagatttc aggatagagt tcttgattga atactgcttg
      481 tgaaggctgt ttgacggcca tatctaatgc atttgcatct tttacaccga tcatttcgcc
      541 tggcgagtga cgtaaaataa ctttcgcgtt gattttgata atttctttag tcgaaagata
      601 cctcataaat caaccaaatc ttttaacgcg tcttcatatt gattcatgat cgaatcaact
      661 aatgaatcaa tatcaggttc ttcttgtgct ttgatcattc gtacagaacc atcagattga
      721 aaaacataac tgattgcgtc acctgtctgc acacccatga attcttttac ttctggtggg
      781 atagttgtta catcgctatt gccagatttt ctaattttat actttttcat ttttttcata
      841 tttcgccacc ctctatacca ttctcattct atagtaagta cattatatcc aagtatatac
      901 ttttaatcaa tgtatatact tttattttgc tttttcttac gaaaaaatga caaagaaaaa
      961 agtttgtgac atgacttttg tcacaaactc tcgtattctt aaccaaaata ttgatgaaat
     1021 acttgtgtaa taactggtag aaaataaata atgatcatag ccgctaccac caccaacaat
     1081 tgttttcgct gtttctttcg ctttagcatg cttgtcgttt tatgaa
//
LOCUS       DACDNO010000181         1090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDNO010000181 DACDNO010000000
VERSION     DACDNO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.193"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..979
                     /locus_tag="IWI22_002921"
     CDS             5..979
                     /locus_tag="IWI22_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9170224.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          383 a          135 c          187 g          385 t
ORIGIN      
        1 aactatgtgt acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga
       61 ttatgaaata tcttacaatg aagtagttac tgttactcca agaaattata agttgaattt
      121 tcgaaaggta aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc
      181 tgactaccct ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa
      241 tttttctggg tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt
      301 tgaatttatt ccttggattt taggacaatg ctcaacagta ggagaaacta aaaaattgtt
      361 aaaaaatatc aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca
      421 ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca
      481 tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt
      541 taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca
      601 aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc
      661 ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc
      721 agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg
      781 tttgtgtgat gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt
      841 tgacaaagga atctattatt atcgaacata tgaagacagt caaattactg caattgatat
      901 gaataaagaa gacttagata gtcataagtt aattagttat ccaattatag aaaaacaaca
      961 aattaaatat ataaattagt taatgtgttg tgattgatta tttaatatag atataataca
     1021 aaaaggcaaa gatattttca aaacattttt tcttgatgtg atatccttgt cttttttgta
     1081 atgtttttaa
//
LOCUS       DACDNO010000182         1088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDNO010000182 DACDNO010000000
VERSION     DACDNO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.194"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..278
                     /locus_tag="IWI22_002922"
     CDS             <1..278
                     /locus_tag="IWI22_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9170225.1"
                     /translation="RNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQ
                     GGKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
     gene            621..877
                     /locus_tag="IWI22_002923"
                     /pseudo
     CDS             621..877
                     /locus_tag="IWI22_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
BASE COUNT          378 a          164 c          198 g          348 t
ORIGIN      
        1 aacgtaatcc cttcgtatct gaatatgtta aacgacttgc aaaaggtatt tgtcaacttt
       61 gccaagaaaa agggccattt ataaaagacg gtgtacctta tttacattgt catcatatag
      121 aatatttgtc gcaaggtgga aaagatgtga ttgaaaactg tatcgcgtta tgtcccaatt
      181 gtcatgctag aattcatgaa cttgagttac ctgacgataa agaaaaattg ctgaagatcg
      241 ttgaaaaaag agaaaatagc ttttctaaaa taccgtaatt tatttttata ggacaaataa
      301 gctaatgtga gctatgcctt ctatataaga ctaagttcta ttataagtaa cgaagtaatt
      361 ctcgttcctt cctgtagtaa tattttttaa ataacctata ggcttcttca aaagtacatc
      421 ctccagtaaa ttctaagtac tggaggattc tttttctagt gttgctttac tcatgaaaaa
      481 gtagttggct acttcatcaa aatttctgaa cttttttaat gtataataaa gagctcgatc
      541 atttactaca aggatgctag ctcctacatt ttcacagttt tttagcaagt aaatttttct
      601 aaaatcacat caggaaatgc atgagattct ttaagaaagg caattatagg gctatgatta
      661 cggttcgtgt aagtgacgcc aaaaaagaat ggctttctta tatggctgaa ttttatggga
      721 ttactttatc tgaacttcta aaaaactatt cgatggaaca actagaagat gaatacaatc
      781 gtcaaacggc tgaaattgcc cataaacatt ggataagaag tgggaaaaca acagtattca
      841 tggaagaatc cttaatgaat ttggtgggct ggaatgacct gccgtttaaa attcacgccc
      901 gaaattcaga agcagctctg attggcaaat acgtgtattg gtagagatag aagataatca
      961 attcgttgtt accgctattc aagtagggca ccgaagaaat gtctatgatt aagagactgt
     1021 ttatttttac aaaaaaataa taactggaat ctagttaaaa ctcttgaagt gagagatttg
     1081 actagatt
//
LOCUS       DACDNO010000183         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDNO010000183 DACDNO010000000
VERSION     DACDNO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.196"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWI22_002924"
     CDS             complement(36..992)
                     /locus_tag="IWI22_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170226.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACDNO010000184         1010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDNO010000184 DACDNO010000000
VERSION     DACDNO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.198"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>1010
                     /locus_tag="IWI22_002925"
     CDS             82..>1010
                     /locus_tag="IWI22_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9170227.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAP"
BASE COUNT          348 a          182 c          194 g          286 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa
//
LOCUS       DACDNO010000185          989 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDNO010000185 DACDNO010000000
VERSION     DACDNO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.199"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            647..964
                     /locus_tag="IWI22_002926"
     CDS             647..964
                     /locus_tag="IWI22_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9170228.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
BASE COUNT          344 a          123 c          142 g          380 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgat
//
LOCUS       DACDNO010000186          945 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDNO010000186 DACDNO010000000
VERSION     DACDNO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.200"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(653..796)
                     /locus_tag="IWI22_002927"
     CDS             complement(653..796)
                     /locus_tag="IWI22_002927"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170229.1"
                     /translation="MEKEAFDLHVLGTPPVFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            716..>945
                     /locus_tag="IWI22_002928"
     rRNA            716..>945
                     /locus_tag="IWI22_002928"
                     /product="16S ribosomal RNA"
BASE COUNT          363 a          140 c          179 g          263 t
ORIGIN      
        1 ttcctatcgc aaaggcagaa gacaagtaaa gcatgtgctt tacttgtttt tttatatatc
       61 caacaaagaa taaggtacaa aatacaagca agttagaaat tcagggatgt cattataaaa
      121 aaaactagcg aacaattaaa tagaataaca tccgttaatt aaacgtaagt agcttttaga
      181 tgaacgaaaa aacaatgaaa tgctgtaata ttcgtgttta aaaataataa ataaaaatag
      241 ggaattttct attgcaattt tataaagaga ttgctatatt attatttgtt ctaaatgatt
      301 cctttaaggt gtcactaaaa attataaaaa atagggttga cgcacttttg atgtcatgtt
      361 ataataacga agtcgtttgg atgaggtagc taataaagct tgattttatc gaaaaaaact
      421 tcgaaaaagt tgttgacaaa taacaataaa gatgatatga taatcaagtc gctgctaatg
      481 agtgacaaag acgtttagaa aaaataaaca aagaagttgt tgacattctc ttctgaatct
      541 gataagatta aagagttgtt gaaacaaaaa gattagacct ttgaaaactg aacaaagtaa
      601 gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga caacaaacat ttttaacaag
      661 caagcaatat gctagcaaac aaattgagct taacaatcgc aagattgttc gaacttttta
      721 tgagagtttg atcctggctc aggacgaaca ctggcggcgt gcctaataca tgcaagtcga
      781 acgcttcttt ttccaccgga gcttgctcca ccggaaaaag aggagtggcg aacgggtgag
      841 taacacgtgg gtaacctgcc catcagaagg ggataacact tggaaacagg tgctaatacc
      901 gtataacaat caaaaccgca tggttttgat ttgaaaggcg ctttc
//
LOCUS       DACDNO010000187          937 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDNO010000187 DACDNO010000000
VERSION     DACDNO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.201"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..831)
                     /locus_tag="IWI22_002929"
     CDS             complement(61..831)
                     /locus_tag="IWI22_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HAP9170230.1"
                     /translation="MNPTLITKKELLKKLDISTGVLADLIRNGMPKEGEMFNLDKIIT
                     WRENWSKNILGELEVGRVYTNKEISEKFKCFKQGGMRRSHQTNTLVLFSDQTGSNVYK
                     DKWLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYL
                     AGQIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDIYNQEENQTRHIRNLSDAKLE
                     EEARKVSNYNKKMAAKYVNEEEFCFSKTKIYKRTRGAS"
BASE COUNT          290 a          165 c          103 g          379 t
ORIGIN      
        1 attaagtcga gcacaacgac gttttgaagg aactttgaga tattctttag gatattaaaa
       61 ttaactagca ccacgggtac gtttatatat ttttgttttt gagaagcaaa actcctcttc
      121 gttaacatat tttgcagcca tttttttatt ataattacta acttttcttg cttcttcctc
      181 taatttagca tcagataaat tacgaatatg tctagtttga ttttcttcct ggttatatat
      241 atctttatct tcaatcaatt gctcttgatt tattaaagct aatggaaatt tatatacttt
      301 tctgctgttg cccgaactat ctttttcatt aacagtatat atttgtcctg ctaaatatac
      361 ttctcccaga tacgtgtgtt cttttggttt aaatgtttca aataaatgaa tctttacaaa
      421 attacttttt gaattggcta aaactttatt ttgattctta tccaatactt gatccccttt
      481 tagccccata ccggtatatt gtaaaatacc atttaaccac ttatctttat aaacattact
      541 tcctgtttga tcactaaaca gtacaagagt attagtttga tgagatcttc tcattccacc
      601 ctgtttgaaa catttaaact tctctgaaat ttctttgtta gtatatactc taccaacttc
      661 taactcacct aatatgtttt tagaccaatt ttctcgccat gtaataatct tatcaagatt
      721 aaacatttct ccctcttttg gcatgccatt tctaattaaa tcagctaata caccagtaga
      781 aatatctaat ttttttagta attctttctt agtaatcaat gttggattca taacaatgca
      841 gctcctaatg atatcaatat aattgcatta gataacttga aaaatcgaaa aaaactcatt
      901 ttgtgtcaag ttatctgtaa tatataaaaa catcatt
//
LOCUS       DACDNO010000188          859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDNO010000188 DACDNO010000000
VERSION     DACDNO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.202"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            28..>859
                     /locus_tag="IWI22_002930"
     CDS             28..>859
                     /locus_tag="IWI22_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9170231.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          297 a          149 c          165 g          248 t
ORIGIN      
        1 ccaaagaatt ataaggagtg agtacaaatg acctataccc atcttacaac ggatgaactt
       61 gtaataatag agtcttattt caaaatgaat caatctgttg ctaaaactgc ccattgcttg
      121 aatcgttcaa gacaaacgat ccataaagta tatctgttct tcaagcaagg aaaatcagcc
      181 ttagaatatt atcaacagta taagaaaaac aaatcaaact gtggtagacg tccgcttgtt
      241 ttacccgagg aacaatcaga atatattcaa agaaaggttg ttcaaggatg gacacccgat
      301 gtgattgttg gtcgtgcagc gtttcctatt agttgttctg ctcgtaccat ttatcgtatg
      361 ttcaaaaagg ggctatttga ttcttctgac ttaccgatga aaggcaagcg taaaccgaat
      421 ggacatcaag aaagacgtgg aaaacaaact ttccgccgct ctattcatga acgtgaaaag
      481 gattatagcc aattctcaaa tgagtttggt caccttgaag gtgacactat cgtaggtctg
      541 aaacataaaa gtgctgtaat taccttagtt gaacgattat caaaagttat catcacattg
      601 aaaccgtgtg gtagacaagc gattgatatt gaaaaaaaat taaatcaatg gtttgaatct
      661 gtaccgaaaa acctattcaa atccatcact tttgattgtg gaaaggaatt ttcaaattgg
      721 aaacagatca gtaatgtcaa tgatattgcc atttatttcg ctgatccagg aacgccgtct
      781 caaagaggcc taaacgagaa ttctaacgga ttgttacgta gagatggttt attgaaatct
      841 atggatttca attcagtag
//
LOCUS       DACDNO010000189          851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDNO010000189 DACDNO010000000
VERSION     DACDNO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.203"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>851)
                     /locus_tag="IWI22_002931"
     rRNA            complement(<1..>851)
                     /locus_tag="IWI22_002931"
                     /product="16S ribosomal RNA"
BASE COUNT          175 a          271 c          188 g          217 t
ORIGIN      
        1 catctcacga cacgagctga cgacaaccat gcaccacctg tcactttgcc cccgaagggg
       61 aagctctatc tctagagtgg tcaaaggatg tcaagacctg gtaaggttct tcgcgttgct
      121 tcgaattaaa ccacatgctc caccgcttgt gcgggccccc gtcaattcct ttgagtttca
      181 accttgcggt cgtactcccc aggcggagtg cttaatgcgt tagctgcagc actgaagggc
      241 ggaaaccctc caacacttag cactcatcgt ttacggcgtg gactaccagg gtatctaatc
      301 ctgtttgctc cccacgcttt cgagcctcag cgtcagttac agaccagaga gccgccttcg
      361 ccactggtgt tcctccatat atctacgcat ttcaccgcta cacatggaat tccactctcc
      421 tcttctgcac tcaagtctcc cagtttccaa tgaccctccc cggttgagcc gggggctttc
      481 acatcagact taagaaaccg cctgcgctcg ctttacgccc aataaatccg gacaacgctt
      541 gccacctacg tattaccgcg gctgctggca cgtagttagc cgtggctttc tggttagata
      601 ccgtcaaggg atgaacagtt actctcatcc ttgttcttct ctaacaacag agttttacga
      661 tccgaaaacc ttcttcactc acgcggcgtt gctcggtcag actttcgtcc attgccgaag
      721 attccctact gctgcctccc gtaggagttt gggccgtgtc tcagtcccaa tgtggccgat
      781 caccctctca ggtcggctat gcatcgtggc cttggtgagc cgttacctca ccaactagct
      841 aatgcaccgc g
//
LOCUS       DACDNO010000190          800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDNO010000190 DACDNO010000000
VERSION     DACDNO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.204"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..693
                     /gene="tnpB"
                     /locus_tag="IWI22_002932"
     CDS             <1..693
                     /gene="tnpB"
                     /locus_tag="IWI22_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9170232.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          295 a          123 c          148 g          234 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct
//
LOCUS       DACDNO010000191          789 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDNO010000191 DACDNO010000000
VERSION     DACDNO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.205"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(150..>789)
                     /locus_tag="IWI22_002933"
     CDS             complement(150..>789)
                     /locus_tag="IWI22_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9170233.1"
                     /translation="GVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQSFVQRGLFG
                     VKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVKDELKSIFKA
                     SELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRRRIRTTNVLE
                     RLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT          238 a          181 c          137 g          233 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacct
//
LOCUS       DACDNO010000192          759 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDNO010000192 DACDNO010000000
VERSION     DACDNO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.206"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(597..740)
                     /locus_tag="IWI22_002934"
     CDS             complement(597..740)
                     /locus_tag="IWI22_002934"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170234.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            659..>759
                     /locus_tag="IWI22_002935"
     rRNA            659..>759
                     /locus_tag="IWI22_002935"
                     /product="16S ribosomal RNA"
BASE COUNT          310 a          124 c          133 g          192 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgtgcctaa tacatgcaag
      721 tcgaacgctt ctttttccac cggagcttgc tccaccgga
//
LOCUS       DACDNO010000193          679 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDNO010000193 DACDNO010000000
VERSION     DACDNO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.207"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          240 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatt
//
LOCUS       DACDNO010000194          627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDNO010000194 DACDNO010000000
VERSION     DACDNO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.208"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            330..>627
                     /locus_tag="IWI22_002936"
     CDS             330..>627
                     /locus_tag="IWI22_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9170235.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFP"
BASE COUNT          230 a           69 c          104 g          224 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaa
//
LOCUS       DACDNO010000195          616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDNO010000195 DACDNO010000000
VERSION     DACDNO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.209"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>616
                     /locus_tag="IWI22_002937"
     CDS             <1..>616
                     /locus_tag="IWI22_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAP9170236.1"
                     /translation="KALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAK
                     TTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVL
                     VKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAES
                     TYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          212 a          105 c          117 g          182 t
ORIGIN      
        1 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
       61 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      121 aatgaagcca aaacgacaaa cgtattgaat cgtaaattct taagagacaa cccattagaa
      181 gcgatcgtaa cagacttgac ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc
      241 attttggatc tgttcaatcg agaaattctc ggctattctt gtggagaaca taaagatgcc
      301 gttctagtaa aaaaagcatt tagccgtatc aaacaacctc tgacagaggt tgagattttt
      361 catactgatc gtggaaaaga gtttgataac caagctattg atgaattatt aacaactttt
      421 gacatcaatc gatcattgag tcataaaggc tgtccttttg ataatgccgt agctgaatca
      481 acttataagt cgttgaaagt agaatttgtc tatcaataca catttgaaac cttacaacaa
      541 ttggatttgg agttatttga ctatgtcaat tggtggaacc accttcggtt gcacggtaca
      601 cttggctacg agacac
//
LOCUS       DACDNO010000196          610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDNO010000196 DACDNO010000000
VERSION     DACDNO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.210"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..575
                     /locus_tag="IWI22_002938"
     CDS             <1..575
                     /locus_tag="IWI22_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9170237.1"
                     /translation="LKIILENMKYNIGKYKERDIYINECIEIVDSLTKNISQILSVHS
                     IENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENVYIGKTALKIILSNL
                     ISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVKFDLNKENSNGLGLY
                     IVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          275 a           34 c           87 g          214 t
ORIGIN      
        1 gtcttaaaat aatactggaa aacatgaaat acaatattgg aaaatataaa gaaagagata
       61 tttatattaa tgaatgtatt gaaatcgttg atagtttaac aaaaaatatt tctcaaatat
      121 tatcagttca ttctatagaa aatttgaata atgatgaaga atatttgaaa ataaatgata
      181 cattagaaga catattaaaa aagtatgaaa tattagcaaa tcaaaagaaa ataactgtca
      241 ataattatat attagacgag aatgtttata taggaaaaac agctttaaag attattctat
      301 ctaatttgat tagtaatgcg gtaaaatata ctgatgtaaa tggggtaatt aatattggga
      361 tagttaatga ttggttatat atagagaatt catacggtaa caataaaatt tcgaatatgg
      421 ataaaatatt tgatgttaaa tttgatttaa ataaggaaaa tagtaatgga ttaggtttat
      481 atattgtaag taatattctg aataattaca atatagaata taaagtattg caggatgaag
      541 aattttttat ctttaaaata aaaatatttt cttaattaag atataatttt gcaatagtta
      601 ttgaaaaata
//
LOCUS       DACDNO010000197          558 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDNO010000197 DACDNO010000000
VERSION     DACDNO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.211"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..411)
                     /locus_tag="IWI22_002939"
     CDS             complement(<1..411)
                     /locus_tag="IWI22_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP9170238.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          195 a           73 c           65 g          225 t
ORIGIN      
        1 gttattaact tgattaaaat cagagataat atcatttaat tcagtttgaa gggttgtaac
       61 gattaaatgg aaattttctt catctgtttt attatatggg gatgttttcg ttatatataa
      121 caacttttct ttttcaattt tcattaataa ctctgtcgaa gtctgtactt tatcttgata
      181 attaaatgca gacataccac tttgtataat tgtaattatt gctgaagcaa ttgaaacaat
      241 caatagtaaa gacctgtgtt cggaagcctg atcaattaat attgggatac tagctgataa
      301 gactattttt ataatatttc cgataccaat tactctatta taaaatcgaa tttctttttt
      361 taatctatcg attgtagaat caatagattc gataaacgct ttttgatcca tcattttctc
      421 tccaatcatt ttattaaaat cattaacaaa aatattataa taggatttaa gtaagttcta
      481 actaatcaca tataaaaata aaaaaacatt tgtattttat caaagtttta ttatacttgt
      541 caatttgtgt acatccta
//
LOCUS       DACDNO010000198          548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDNO010000198 DACDNO010000000
VERSION     DACDNO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.212"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>548)
                     /locus_tag="IWI22_002940"
     CDS             complement(<1..>548)
                     /locus_tag="IWI22_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9170239.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DACDNO010000199          546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDNO010000199 DACDNO010000000
VERSION     DACDNO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.213"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            131..418
                     /locus_tag="IWI22_002941"
     CDS             131..418
                     /locus_tag="IWI22_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170240.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT          196 a           87 c           99 g          164 t
ORIGIN      
        1 ctttttttgt gaaaagtaca attagttgtc tttcttcgaa ttttttgtca caataagcag
       61 caaagataaa gctgttacat aaaagaacaa gcagctttat ttgattttga aaagaaaggt
      121 gagaaagaag atgaataaag aatattttgt ccaacaagat gcatttttag aagattataa
      181 aagtgcttcg aagaaattaa cgatcaatat tgatgagttg gcagatcaag tactctctca
      241 gttgaatgaa acgcaagagg aatattacac gatgccagca aatgacgcca aagataatgt
      301 ttctcatcgt tttcctttca gtagacgcat ctactcagat gaaaatgacg gcacattgaa
      361 tacagagtac acatatgaag gtcagccata tgtgctagaa actgatgatg atctttaaag
      421 acagtttttg aaaaggccgc tcatccttaa gtgatgacaa aaaatcatca caattaccga
      481 tgactgtctc tttactatct tttaatcaga aaaaggggtt atgacaagct ttgtcaaccc
      541 cttttt
//
LOCUS       DACDNO010000200          539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDNO010000200 DACDNO010000000
VERSION     DACDNO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.214"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(264..527)
                     /locus_tag="IWI22_002942"
     CDS             complement(264..527)
                     /locus_tag="IWI22_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP9170241.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          212 a           93 c           75 g          159 t
ORIGIN      
        1 tgaatttttt catgttgaaa acatcactaa tcaaaggcaa attataattt agaaatagaa
       61 tgaaaacact aaatgaaaat agaacattag aaaaaataga atcaaaaaaa cccaagacta
      121 gtgccgaaat aagggaaaca atcgaagcta caaaaaataa tgatttcgtt gaacaggacc
      181 aatttatttg tttccgatat ttctcaaaaa gaactttctt tgggaaagtt aaaggcgact
      241 gtaaagtcgc ctttagtcct tttttaccaa gctgctgcat atttagctcc atatttttta
      301 attaatgatt tggctgtagc aacaatacca gctgtaatta atccgcctcc aacaactgca
      361 gcaacaatag aaatgattgt agcaaccgtg ctaccagtat taattatgtc aataactttt
      421 ttagctgcat aagaagaaat tccaatgtta gcacttagca ttggtacacc aacaaagaat
      481 cccgcaaaac ctaaaattgg taaaactaat aataaattct ttttcataat aattcctct
//
LOCUS       DACDNO010000201          528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDNO010000201 DACDNO010000000
VERSION     DACDNO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.215"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(5..90)
                     /locus_tag="IWI22_002943"
     tRNA            complement(5..90)
                     /locus_tag="IWI22_002943"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IWI22_002944"
     tRNA            complement(107..178)
                     /locus_tag="IWI22_002944"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IWI22_002945"
     tRNA            complement(190..262)
                     /locus_tag="IWI22_002945"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IWI22_002946"
     tRNA            complement(280..361)
                     /locus_tag="IWI22_002946"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IWI22_002947"
     tRNA            complement(369..441)
                     /locus_tag="IWI22_002947"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IWI22_002948"
     tRNA            complement(445..517)
                     /locus_tag="IWI22_002948"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACDNO010000202          513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDNO010000202 DACDNO010000000
VERSION     DACDNO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.216"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWI22_002949"
     CDS             complement(<1..132)
                     /locus_tag="IWI22_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9170242.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          185 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag aca
//
LOCUS       DACDNO010000203          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDNO010000203 DACDNO010000000
VERSION     DACDNO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.217"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..434)
                     /locus_tag="IWI22_002950"
     CDS             complement(<1..434)
                     /locus_tag="IWI22_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9170243.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
BASE COUNT          146 a           85 c           80 g          199 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc
//
LOCUS       DACDNO010000204          486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDNO010000204 DACDNO010000000
VERSION     DACDNO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.218"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>486)
                     /locus_tag="IWI22_002951"
     CDS             complement(<1..>486)
                     /locus_tag="IWI22_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9170244.1"
                     /translation="LYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLN
                     QCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMR
                     WTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTS
                     L"
BASE COUNT          127 a          107 c           84 g          168 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat gactcgttga tgatttttac ttatggtaca ttggtttagc
      361 agaggacagt tcacacattc ttttggattg gatttgtatt ctcggtaccc ttctcgagta
      421 accgttgaaa aagcgagcat ttgatcttgc ggacaaatat aacaatcaaa atactcgtca
      481 tagaga
//
LOCUS       DACDNO010000205          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDNO010000205 DACDNO010000000
VERSION     DACDNO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.219"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>479
                     /locus_tag="IWI22_002952"
     CDS             <1..>479
                     /locus_tag="IWI22_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170245.1"
                     /translation="IFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKW
                     PEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDASEGIKNKEDL
                     PKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG
                     "
BASE COUNT          192 a           74 c          113 g          100 t
ORIGIN      
        1 atcttcaccg caggggaagg aacaagtatt gaaagtggaa caacggtctt tgcagtgaaa
       61 gacggcgtaa gcttaccaga agataaactt ccagtgttga aagcaaaaga tggttataca
      121 gatgcgaaat ggccagaaga agcaactcaa ccaattacag cagatgatac agaatttgta
      181 tcaagtgcaa caaaactgga tgataaatct gacgctgaca aatataatcc tgaaggtcaa
      241 aaagtgacta cagaattgaa taaggaacca gacgcatctg agggaattaa gaacaaagaa
      301 gatttaccaa aagatactaa gtatacttgg aaagaaaaag tagatgttag cgcagctgga
      361 aataaaaaag ggactgttgt agtgacatat ccagatggat catctgatga agttgaagta
      421 gatgttacag tgacagacaa tcgttctgac gccgataaat atgagccaac agtagaagg
//
LOCUS       DACDNO010000206          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDNO010000206 DACDNO010000000
VERSION     DACDNO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.220"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            7..>479
                     /locus_tag="IWI22_002953"
     CDS             7..>479
                     /locus_tag="IWI22_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9170246.1"
                     /translation="MQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGF
                     NSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEK
                     GVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDAT"
BASE COUNT          174 a           87 c           80 g          138 t
ORIGIN      
        1 gaaattgtgc aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta
       61 gaaaaagcga taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa
      121 tatgatcgca ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc
      181 aaaaccgaat atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa
      241 caacaaactt taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat
      301 ttattcgaaa aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt
      361 catcactata ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat
      421 gcctttaaat ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctactta
//
LOCUS       DACDNO010000207          452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDNO010000207 DACDNO010000000
VERSION     DACDNO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.221"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>452
                     /locus_tag="IWI22_002954"
     CDS             <1..>452
                     /locus_tag="IWI22_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9170247.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKT
                     GFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLAL"
BASE COUNT          152 a           87 c           77 g          136 t
ORIGIN      
        1 caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt
       61 aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac
      121 tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt
      181 ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt
      241 ggctataact caacaaaaaa atcttatttt tatggtctta aaattcatat gatcgttact
      301 aagactggct ttccgattac ttactcaatc acgaatccag gtgtccatga cgtgaaagtg
      361 ttagagactt tatcggaaga agcgaatctt cccaatatcc taggggacaa aggatatatt
      421 agccataaaa tccatgaaaa gcttgccctt aa
//
LOCUS       DACDNO010000208          446 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDNO010000208 DACDNO010000000
VERSION     DACDNO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.222"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            92..391
                     /locus_tag="IWI22_002955"
                     /pseudo
     CDS             92..391
                     /locus_tag="IWI22_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
BASE COUNT          151 a           72 c           67 g          156 t
ORIGIN      
        1 caaatcatta gaatcgtgat aagacattgt attaaatcta atcctttatc cattttttat
       61 caatcttact tcgcaccatc tccaatactt attattagcc tctcttggag catgtaaagt
      121 tattgtagct cggtctttcg ctaaagtgca gggcataaat ttaaacgata tccgtgtaga
      181 gatagatgga gtattagatc ctgatggttt tataggaaaa aataaagcag ctaaaatagg
      241 ctattctgca atcgtatcta aattttatat tgaagctgat aatactgatg aagagatttc
      301 agattttatc gcatttattg atcgtacttg tccggtagct gataccatta aaaatgcacc
      361 tacgactaaa tcagaaatcc ataaattata aactatgaat taaaaaagat tcttctcatt
      421 ttgcactaaa caggtatttc tatttt
//
LOCUS       DACDNO010000209          427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDNO010000209 DACDNO010000000
VERSION     DACDNO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.223"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWI22_002956"
     CDS             141..398
                     /locus_tag="IWI22_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9170248.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          186 a           53 c           60 g          128 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgt
//
LOCUS       DACDNO010000210          423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDNO010000210 DACDNO010000000
VERSION     DACDNO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.224"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            55..423
                     /locus_tag="IWI22_002957"
                     /pseudo
     CDS             55..423
                     /locus_tag="IWI22_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          169 a           69 c           81 g          104 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atc
//
LOCUS       DACDNO010000211          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDNO010000211 DACDNO010000000
VERSION     DACDNO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.denovo.225"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..397
                     /locus_tag="IWI22_002958"
     CDS             <1..397
                     /locus_tag="IWI22_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9170249.1"
                     /translation="KTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFIS
                     KNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHE
                     DSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          186 a           62 c           45 g          111 t
ORIGIN      
        1 taaaacaaca actaaaaata caggttctta tagaaccatt gaattatcaa gtaatgctaa
       61 agaaatcata gatcaattaa ttgaagaaaa caaattaatt aagaataata aagataaata
      121 cattttcatc tctaaaaatg gtaacccgat atctattcaa gcatataatc aatctttaag
      181 agcagtatcc aaagaattaa acatagataa aaaagtttct tctcacatgt taaggcattc
      241 ccacatttct ctacttacag aattaggaat cccattaaaa gccattatgg atcgtgtagg
      301 acatgaagat tcaaaaacaa cattaaagat ttatactcat acaactaaaa atatgcaaaa
      361 tcaactagta gaaaaactag gaaaaataga aatttaaatt gccc
//
LOCUS       DACDNO010000212         5291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.115, whole genome shotgun sequence.
ACCESSION   DACDNO010000212 DACDNO010000000
VERSION     DACDNO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.115"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWI22_002959"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWI22_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9170250.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IWI22_002960"
     CDS             2039..2227
                     /locus_tag="IWI22_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9170251.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="IWI22_002961"
     CDS             complement(2285..2638)
                     /locus_tag="IWI22_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9170252.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="IWI22_002962"
     CDS             3169..3591
                     /locus_tag="IWI22_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9170253.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="IWI22_002963"
     CDS             3588..3818
                     /locus_tag="IWI22_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9170254.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="IWI22_002964"
     CDS             4316..4516
                     /locus_tag="IWI22_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9170255.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..>5291
                     /locus_tag="IWI22_002965"
     CDS             4543..>5291
                     /locus_tag="IWI22_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9170256.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASV"
BASE COUNT         1820 a          845 c         1006 g         1620 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg t
//
LOCUS       DACDNO010000213         5291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.116, whole genome shotgun sequence.
ACCESSION   DACDNO010000213 DACDNO010000000
VERSION     DACDNO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.116"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWI22_002966"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWI22_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9170257.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="IWI22_002967"
     CDS             2039..2227
                     /locus_tag="IWI22_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9170258.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..2638)
                     /locus_tag="IWI22_002968"
     CDS             complement(2285..2638)
                     /locus_tag="IWI22_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9170259.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            3169..3591
                     /locus_tag="IWI22_002969"
     CDS             3169..3591
                     /locus_tag="IWI22_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9170260.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            3588..3818
                     /locus_tag="IWI22_002970"
     CDS             3588..3818
                     /locus_tag="IWI22_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9170261.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            4316..4516
                     /locus_tag="IWI22_002971"
     CDS             4316..4516
                     /locus_tag="IWI22_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9170262.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            4543..>5291
                     /locus_tag="IWI22_002972"
     CDS             4543..>5291
                     /locus_tag="IWI22_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9170263.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASV"
BASE COUNT         1819 a          845 c         1007 g         1620 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatgt atttttctac ctgtaacctt cgagtgcttt
     2401 taatcaaatt atctgtaggt aagaaaaatt catcaataga tacatggagt aatgatacaa
     2461 gatcatacag cacttgtgta cttgggtgtt gtcctttgtt ttctatattt gttaaataac
     2521 gtgggtcaat ttcaatcatt gttcccactt gttcacgagt gagaccccgt ttcattcgag
     2581 cctctttaat cgctaaacca acggctctaa aatcgtattt atcttctttt ttacgcatac
     2641 taaatcacct ctatacattt tactgttcct atataaccat aaacaggtat agaaaaacat
     2701 gttatatagt ttatgagttc ctatagaaca ctgtggaatt tattaatgcg atgaatccaa
     2761 tttcaaatag aaaggtcgct gattttattt acctatacaa ctttataaaa tttagtcata
     2821 ctcctagaaa gtagataaaa ttggcgactt aatggaggga aaagtcgcca tgagaaagat
     2881 aatctgtcat ctagttctct taattaagtt cttatcaaaa aaacttatcc acctgtactg
     2941 gatagatatg accatcaatg tggttttgca gattacatag atagaaaaat caaagcatat
     3001 gaacagagaa aacaatctgt ttatgtgctt ttttggtcac ttaataaata tcttttagga
     3061 tttctcttca tttatgcaac aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat
     3121 aaagaagcac atgccactcc ctttcacgac agaaaggggg tgaaaagcat gaaaccatct
     3181 tcttttcaag aaaccataga aaatcagttt gactatatct gcaaacaagt tattgaagat
     3241 gaacgaaaag attacttcaa acatctagac agactatcag caaaagaagt ctcgttttct
     3301 gatatagata actaccttgt caactctttt tctactgttg atacgtacgt cactgatttc
     3361 caactattta aattatttgg attaagaatc aatgttgaaa gtgatttatt aagtgaagcc
     3421 ctgcgtcatc tttcagagaa aaaacggaac attattttac ttcattactt tatggatatg
     3481 agtgatgtgg agattgcgga gttactaaat ttgaatcgtt cgactgttta tcgtcatcga
     3541 ataagtggac tagccatgat taaagaattc atgaaggagt gtaaagaatg aaaacagctt
     3601 atccaagggt tcccttcccc ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta
     3661 atcagattgt taaacattat cgagggtata cctcaaaacg ttcattacgt cgcatgacag
     3721 acgaatatgg aaattctcat atggttattg atgaaaccct acgtggcaga atggaaacga
     3781 aactcatctc taagatttta tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt
     3841 gtcgaatcgt atccacgctt cccgatttgg gaggtttgtt attccatcaa agcatattgt
     3901 gtgttcagta ggttttgata ggctaacaag ccaaacgttc tttgacaacc aaataaaggc
     3961 aatcgaatac gtttcggtaa gaaaagagct gatgaactaa cacgccatga cctacaaaat
     4021 tagtgagcga ttcatgttag taattcgaaa gtaatcttta gcaggattgc tgccatgact
     4081 ttcttatcgt gataatgata ctcccgtata gccatagtcc gagcgttaga agcgtcacag
     4141 gcaatgggta cggctacatg agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga
     4201 gctgttcgat tgctaagata ttcttctctt gtcagtgtat tattataaaa ctatttgata
     4261 taattatagg tgaatattgg agttttccta gcctataatt gggaaaggag taataatgat
     4321 aacggtcaca aaaaaggatt tagttgagct aggatatgga acttcttttt cagcagacat
     4381 cataaaaaaa gcaaaagaat tgatgattac aaaagggcat atctactatc aatctcgaaa
     4441 gctagataga gtccctcgtg aagctgttga agaaattcta ggaattcggt tttcaaatga
     4501 aaacaggtct gagtagtttt tgcacagtaa ggagtaaaaa ctatggttaa agacccaata
     4561 aaaaaagcaa agaatggaac ctattatttt cgtgcaaatc taggttatga ttctaaaggg
     4621 aaaaaaattc aaaaatatcg tagtggattc actactaaaa aagaagcaag agaagaatat
     4681 tcgaaactgc tattgatgaa accagaagaa ttaagtgaga ataaggataa aatgacgttt
     4741 aaacattata tctttgaaat tttccttcca tggtataaga cacaggtaaa gctcagaaca
     4801 tatgaaaatc gcttaccaac tattaagaag catttctctt attttgataa aatggctgtt
     4861 tccgatattg agcctattga tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag
     4921 tcttcttatg taagagctgt tcaaggacta ttttcagttg ctatggatcg agcaattgtc
     4981 cttgggataa ccaccacaaa tccatctaag attattggca atgtgaaaaa gcaaaagtct
     5041 aaaattgaat tttggacaaa agaagaattt gaaaaagtaa tctctctaat ttacaaagaa
     5101 gattactatc aacacttttt atttatctct ctgtggtttt tatttatgac tggaatgcga
     5161 attggtgaag ctactgccat tcaatgggaa gatattgatt ttgactcagg agtactaacc
     5221 atcaacaaaa cactctatta caagaatcaa tcgaattata gatttgttga accaaaaaca
     5281 aaggcgagcg t
//
LOCUS       DACDNO010000214         4053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.133, whole genome shotgun sequence.
ACCESSION   DACDNO010000214 DACDNO010000000
VERSION     DACDNO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.133"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            208..867
                     /gene="vanR"
                     /locus_tag="IWI22_002973"
     CDS             208..867
                     /gene="vanR"
                     /locus_tag="IWI22_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9170264.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            870..2213
                     /gene="vanS"
                     /locus_tag="IWI22_002974"
     CDS             870..2213
                     /gene="vanS"
                     /locus_tag="IWI22_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9170265.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            2384..3190
                     /gene="vanY"
                     /locus_tag="IWI22_002975"
     CDS             2384..3190
                     /gene="vanY"
                     /locus_tag="IWI22_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9170266.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            3203..4039
                     /gene="vanW"
                     /locus_tag="IWI22_002976"
                     /pseudo
     CDS             3203..4039
                     /gene="vanW"
                     /locus_tag="IWI22_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         1225 a          829 c          994 g         1005 t
ORIGIN      
        1 gctgtaatcg aaacatccca aacattaaga aggtgttaat tattattatc ttgatttaat
       61 tcgtttaaac caataatata gatacataca ccatgaaaag aagcgagtat cccaatgacg
      121 gatgattaaa atccgaaaag aaatcgcttt gaaactacag ggaaactaca gactgttatg
      181 ttatcttctt aaaatggagg gatttttatg tcgatacgaa ttctacttgt cgaggatgat
      241 gatcatatct gtaatacagt aagggcgttt ctggctgagg caggatatca ggtggatgcc
      301 tgcacagatg gaaatgaggc atacaccaag ttttacgaaa acacttatca actggttatt
      361 cttgatatta tgctgcccgg tatgaacggg catgaacttt tgcgtgaatt tcgtgcgaaa
      421 aatgatactc ccattctgat gatgacagcc ctgtcggatg acgaaaacca aatccgggcg
      481 tttgatctag aggcagacga ctatgtaaca aagccattca agatgcagat tttactaaag
      541 cgggtggaag ccctgctgcg gcgcagcggt gcgctggcaa aggaaatccg tgtcggcagg
      601 ctgacacttc tgccggagga tttcacggta ctttgtgacg gtacagagct gcccctgaca
      661 cgaaaagaat ttgaaatcct tttgctgctg gtgcagaaca aaggcagaac cttaacgcat
      721 gaaatcattt tgtcacgtat atggggatat gactttgaga gtgatggcag cacagtccac
      781 actcatatca aaaatttgcg ggccaagctg ccggaaaata tcatcaaaac cattcgcggt
      841 gtaggatacc gattggagga atcataataa tggaaagaaa agggattttc attaaggttt
      901 tttcctatac gatcattgtc ctgctactgc ttgtcggtgt aacagcaaca ctgtttgcac
      961 agcaatttgt gtcttatttc agagtgatgg aattacagca aacagtaaaa tcctatcagc
     1021 cattggtgga actgattcag aatagcgata ggcttgatat tcaagaggtg gcagggctgt
     1081 ttcactacaa taaccaatcc tttgagtttt atattgaaga taaagaggga agcgtactct
     1141 atgccacacc aaatgccaat acatcaaata gttttagacc cgactttctt tatgtggtac
     1201 atagagatga taatatttcg attgttgctc aaagcaaggc aggtgtggga ttgctttatc
     1261 aagggctgac aattcgggga attgttatga ttgcgataat ggttgtattc agccttttat
     1321 gcgcgtatat ctttgcgcgg caaatgacaa cgccgatcaa agccttagcg gacagtgcga
     1381 ataaaatggc aaacctgaaa gatgtaccgc cgccgctgga gcgaaaggat gagcttggcg
     1441 cactggctca tgatatgcat tccatgtatg tcaggctgaa agaaaccatc gcaaggctgg
     1501 aggatgaaat cgcaagggaa catgagttgg aggaaacaca gcgatatttc tttgcggcag
     1561 cttctcatga gctaaaaacg cccatcgcgg ctacaagcgt tctgttggag ggaatgcttg
     1621 aaaatatcgg tgactacaaa gatcattcta agtatctgcg cgaatgcatc aaaatgatgg
     1681 ataggcaggg caaaatcatt tccgaaatac tggagcttgt cagcctgaat gatgggagaa
     1741 tcgtacccat agctgaaccg ttggacatag ggcgcacggt tgccgagttg ctgcccgatt
     1801 ttcaaacctt ggcagaggca aacaaccagc ggttcgtcac agatattcca gccgggcaaa
     1861 ttgtcctgtc cgatccgagg ctgctccaaa aggcactatc caatgtcata ttgaatgcag
     1921 ttcagaacac gccgcaggga ggcgaggtac ggatatggag tgagcctggt gctgaaaaat
     1981 gccgcctttt tgttttgaac atgggcgttc acattgatga tactgcgctt ccaaggctgt
     2041 tcaccccatt ctatcgcatt gatcaggcgc gaagcagaaa aagtgggcga aacggtttag
     2101 gacttgccat cgtacaaaaa acgctggatg ccatgagcct ccagtatgcg ctggaaaaca
     2161 cctcggatgg cgttttgttc tggctggatt taccgctcac atcaacattg taaatattta
     2221 aaacttaaat gattttgacc gacaggtata accctgccgg tctttttttg ctgctacagg
     2281 aaaactacag actggctaca gggaaagtac agatgcgctt gccataataa caatcgtacc
     2341 agccacaaat cgtagtttta ttgcaaagga ggcattcgat caaatggaaa aaagcaacta
     2401 tcattccaat gcggatcatc acaaacggca tatgaaacaa tccgtggaaa agcgggcttt
     2461 tctatgtgcg ttcattattt cgttcacagt ctgcacgctg tttttggggc ggagactggc
     2521 ttccgtattg gaggcaacac agataccgcc catccctgca actcatacag gcagcagcac
     2581 tgacgtagtg gagaatttgg aggaaaacgc tcttgccacc gccaaagaac agggagatga
     2641 acaggaatgg agcctgattt tagtgaacag gcagaacccc atccccgcac agtacgatgt
     2701 ggagcttgag caactatcaa atggtgagcg gatagatatt cggatttctc cctatcttca
     2761 agatttgttt gatgccgcaa gaactgatgg agtttacccg attgtcgcat ccggataccg
     2821 aacaacagaa aaacagcaag aaattatgga tgaaaaaatt gccgaatata aggcgaaagg
     2881 ctacacctct gcacaggcta aagcggaagc agaaacttgg gtggccgtgc cgggaacgag
     2941 cgagcatcag cttggtcttg ctgtggatat caatgcggac ggaattcatt caacaggcaa
     3001 cgaggtttat agatggctgg atgaaaacag ctatcgtttt ggttttattc gtcgctaccc
     3061 accagacaag acagagataa ccggtgtgag caacgagccg tggcattacc gatatgttgg
     3121 catcgaagct gccacagaga tgtacaacca aggggtttgc cttgaggaat atttaaaacc
     3181 agaaaaatga gaaaaggatg ttatggacag aaaaagattg acacagcgtt tcccgttcct
     3241 gcttccaatg agacgggcgc agagaaaaat gtgcttttat gcgggaatga gatttgacgg
     3301 ctgtcgctat gcgcagacga taggggaaaa atcgctttcc catttgctct ttgaaacgga
     3361 ttgcgcatta tacaaccaca ataccggatt tgacatgata taccaggaaa acaaggtgtt
     3421 caacttaaag ctggcagcaa agaccttaaa cggcttattg ataagaccgg gggaaacctt
     3481 ttctttctgg tggctggtac gccatgcgga caaagatacc ccctataaag acggccttac
     3541 ggtgaccaat ggtaaactca ccaccatgtc gggcggcggt atgtgccaga tgagcaattt
     3601 actattttgg atgttcctgc atacgccatt gacaattatc cagcgcagag gtcacgaagt
     3661 aaaggagttt ccagagccaa acagtgacga gattaaaggg gtggatgcaa ccatttcaga
     3721 gggctggatt gatttaaaag tgcgaaacga taccgactgc acctaccaaa tatgagtgac
     3781 cttagatgat gagaaaatca tcggtcaggt gtccgccgac aaagagcccc aagcattata
     3841 caaaattaca aatggcagta tccagtatgt ccgtgaaagt ggcgggattt atgaatatgc
     3901 ccaggttaaa cggatgcaag ttgccttagg taccggggaa ataatagatt gcaagctgct
     3961 ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa agtgtggata ttcagtctaa
     4021 tggtgataag tcaagatgaa atttaagatt ttc
//
LOCUS       DACDNO010000215         3768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.136, whole genome shotgun sequence.
ACCESSION   DACDNO010000215 DACDNO010000000
VERSION     DACDNO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.136"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            239..1306
                     /gene="rlmN"
                     /locus_tag="IWI22_002977"
     CDS             239..1306
                     /gene="rlmN"
                     /locus_tag="IWI22_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9170267.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1499..2602)
                     /locus_tag="IWI22_002978"
     CDS             complement(1499..2602)
                     /locus_tag="IWI22_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9170268.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3155..3703
                     /gene="aac(6')"
                     /locus_tag="IWI22_002979"
     CDS             3155..3703
                     /gene="aac(6')"
                     /locus_tag="IWI22_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9170269.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1245 a          634 c          757 g         1132 t
ORIGIN      
        1 gacatgtaaa acttgtgtga tgaagggttg aaaaacgaat aaccaataaa aagacaaggc
       61 atgatgatac caaaagaaaa aatggttttt ttccatcttt tctaatacgg tatgatgtct
      121 tgtcttttta gtttttgtga gattaaagtt ctattcgatt tatttgtgaa gtattgcatt
      181 aaggaaatga atcatgtata gtatgtagcg aaactttgag aaaaagaggg aaaatacttt
      241 ggaaaaacag tccatttatg gcttgacgaa tgaagaatta atcaattggt ttatagaaaa
      301 cggcgagaaa aaattccgag ctgcccaagt ttgggaatgg ttgtatcaaa aacgtgtaag
      361 taatttcaca gaaatgacga atctatcgaa acaattgatc gaaaaattat cggagcattt
      421 tatcatcaat cctctaaaac agatggtcgt ccaagaagct tcggatggta cagtaaaata
      481 tttgtttgaa ctccctgata aaaacatgat tgagactgta ctgatgagac aagaatatgg
      541 cttgtcagtt tgtgtgacga cacaagtagg atgtaatatc ggctgtacgt tttgtgcaag
      601 cgggctgctc aaaaagaacc gtgatctgac tgctggagaa attgttgcac agatcatgat
      661 ggtccaacat tattttgatg agcgcaatct tggcgaacgc gtttcacatg tcgttgtaat
      721 ggggatcggt gagccatttg ataattacga caatgtaatg gactttttgc atatcatcaa
      781 tgatgccaaa ggattggcaa ttggtgctcg gcacattaca gtttccacta gtggattagc
      841 acacaaaatc aaggaattcg ctaataacgg cttgcaagtc aatctggcta tttccttgca
      901 cgcgccgaat aatgaggtgc gaacatcgat catgcggatc aaccgaagtt tcccaattga
      961 gaaattgatg gaagctgtgg atgagtattt agagaaaaca aaccgtcgaa tcacgtttga
     1021 atatatcatg ttgaatcaag taaatgatag accagagcat gcgcaacaat tagcagatct
     1081 attaaaagat aagaaaaaat tagcatatgt caacttgatt ccatataatc cagtcagtga
     1141 acatgatcaa tattctcgca gtccaaaagc tgatgtattg agattttatg atgtattgaa
     1201 gaaaaacggc gtaaactgtg tgatacgtaa agaacatggt acagatattg atgcagcttg
     1261 cggacaattg cgaagcaagc aaatgaaaaa aacagttgcc aaataatcgt atcctactcg
     1321 ttttttcaat taccataaaa ggagcagttt tcttaattaa ggaactgttc aacttaaata
     1381 agagaaaaga ttctatagcg aaagctataa agcttttttt gacaagaaaa accctcatca
     1441 agcaaagaaa tgtcctcaat ttgatgacag cctgcttgat gggggtttta gcaattattt
     1501 aactgatttt cttatatcct tgctggcttt cttgtgtagc atgataagtt tctacgtcaa
     1561 gaatggcctg tttaaatttt tctcgggtga tttcataagg cacatgttta atatcatttg
     1621 ttgccatggc tttatctaag attgcttcta aatcggtcgt gacattgaga ttcatgtcgg
     1681 ttaacgatac tggaagttgg ttctttttca taaacacata gattttatca aacaaatcga
     1741 actgctgatc catcatcaat aaggtcagta caccataact gacagatgct ccgtgaaggt
     1801 atccctccat tttttctaga acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat
     1861 tatagttatt gttaactaaa acagaaacca atccggtagt aacaatgata tccgaaacga
     1921 cttgttcaat tgcatatgag ggacgatcgt cggcactatc tttcaatgct tgcgtaccat
     1981 aagtcaagag acgatcaatg cacatttttc cgatttggat tcctaatcta tccgtgtagc
     2041 ctagatcatc gccacgagca gaaaaagtag attcgacttc tttggataaa gcatcgccaa
     2101 tacctgccca taagtattta tcaggtgctt cggcaataat tgctgtatca atgaaggtgt
     2161 gtacaggtgg ttttgaagga aagaagagtc cgtccattac gccttcttcg ttatagatga
     2221 cacataccgc agtgacgggc gaacagttcg aaccaatagt gggaaaggtg aagactggtt
     2281 tagctagttt ttcaccaatg acttttacag tatcgcaagc acggccacca ccgactgcaa
     2341 acaggacgtc tgctttttgg acactttcat gctggatcaa ctgttcaata tttccataag
     2401 ttgaattacc cccgtaccag agactggcga caacctctat ttcctgtgtc aaagctactt
     2461 ttattttgtt ttctgccttt gaaagtgcag tcttccctcc aatcagcact acttttgtcc
     2521 catattcttt gacgattcca tttgcttttt catacgcatc tgttccaatg gtataatttg
     2581 gtaagaaaac actttgactc atattcattt ccttctttct tcgttgaaac gggttaaaat
     2641 tttaaagagt gatcgatctg actgcggctg tagatggagt agaggtgaac ctaagacagg
     2701 ataagtgatg aataaggtgg catgaacacc ttgaaatcat caataagtat cctaaaaacg
     2761 aacaagtaca aaaacattga aaacgacaat aatgaatttt tgttcctctt agtatttcta
     2821 ccgattccct ttgctttttt gtaattatct aaaaattcta aaaattaaga ttattatatg
     2881 aaaacaggtt ttatttgtca attcataatt ggtttttttt aaagcaaaag ataatctata
     2941 tcatgatgaa caaacagagt tttttcctcc aattgatggc tttcctgatc tttcctattt
     3001 tgaataaagt gtttctgtat aattttttta ttttataagt aagataaata atgaaagaat
     3061 agagatactg attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga
     3121 ctgttagaat aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa
     3181 taatccagta ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata
     3241 tggagacagc tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc
     3301 agcggtagac caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac
     3361 aggttgggaa ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac
     3421 tcgattagtc aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt
     3481 aggtacggat gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac
     3541 atttgataaa gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa
     3601 attaggttat aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat
     3661 ttggatggca aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac
     3721 agaagtaatc agcttcaaga aataagaagg aatttacgaa aattactt
//
LOCUS       DACDNO010000216         3506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.137, whole genome shotgun sequence.
ACCESSION   DACDNO010000216 DACDNO010000000
VERSION     DACDNO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.137"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWI22_002980"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWI22_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9170270.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IWI22_002981"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IWI22_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP9170271.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1114 a          534 c          716 g         1142 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgatt
//
LOCUS       DACDNO010000217         3506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.138, whole genome shotgun sequence.
ACCESSION   DACDNO010000217 DACDNO010000000
VERSION     DACDNO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.138"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="IWI22_002982"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="IWI22_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9170272.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="IWI22_002983"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="IWI22_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP9170273.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1113 a          534 c          717 g         1142 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgatt
//
LOCUS       DACDNO010000218         3026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.142, whole genome shotgun sequence.
ACCESSION   DACDNO010000218 DACDNO010000000
VERSION     DACDNO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.142"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            47..1018
                     /locus_tag="IWI22_002984"
     CDS             47..1018
                     /locus_tag="IWI22_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP9170274.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="IWI22_002985"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="IWI22_002985"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP9170275.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..2653
                     /locus_tag="IWI22_002986"
     CDS             2045..2653
                     /locus_tag="IWI22_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP9170276.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          814 a          635 c          828 g          749 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttcac gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat
     2341 agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg
     2401 aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag
     2461 taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga
     2521 ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg
     2581 ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct attttaattt
     2641 tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg
     2701 ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa
     2761 attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt
     2821 tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt
     2881 ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg
     2941 ccatgctcta atccacttcc accactcaaa aaagccttgc ccctccatga actgaacccc
     3001 aaaagttgaa ctatttaatg gactgt
//
LOCUS       DACDNO010000219         2197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.161, whole genome shotgun sequence.
ACCESSION   DACDNO010000219 DACDNO010000000
VERSION     DACDNO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.161"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..228
                     /locus_tag="IWI22_002987"
     CDS             <1..228
                     /locus_tag="IWI22_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9170277.1"
                     /translation="PYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPI
                     GLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            486..1988
                     /locus_tag="IWI22_002988"
     CDS             486..1988
                     /locus_tag="IWI22_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9170278.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2055..>2197
                     /locus_tag="IWI22_002989"
     CDS             2055..>2197
                     /locus_tag="IWI22_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9170279.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVP"
BASE COUNT          778 a          388 c          479 g          552 t
ORIGIN      
        1 ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc tatttgcgga
       61 agtagagacc aagagctaga aggactttct gattttctcc caattgtctt aagtattcat
      121 ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga aaaaacaaaa
      181 atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt gacaaaatca
      241 ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg tgcattgaaa
      301 aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca aggaagcagt
      361 gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg tgtgtatcca
      421 acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt acgaggtgat
      481 ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc caaggcacat
      541 tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga cttatcggac
      601 gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta ccttatcaag
      661 ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa gataaagaac
      721 gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa atcgaaagag
      781 agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc acactatcgg
      841 ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact catttcccgt
      901 taatcgatga accaacgaat catttggata tctctggtag aaaacaagta gcggcttatt
      961 tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt atcgatgaag
     1021 tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa gggaatttct
     1081 ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa aatgaaaaat
     1141 tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa tggtctcgtt
     1201 cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa tctagacgag
     1261 tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc aaagcaatcg
     1321 tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat atcgaatata
     1381 tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta agcgtagaag
     1441 atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca atcgagcctc
     1501 atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt atccattatc
     1561 ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa catctgagca
     1621 ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa tttgcagaga
     1681 aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg atggaaagag
     1741 atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa gtggaattgg
     1801 ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg aattatttgg
     1861 atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct gccatgttac
     1921 tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt tctcttgaga
     1981 gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac tttgccaacg
     2041 aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg aacgcacaag
     2101 gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg caggcacaga
     2161 aactagcaga aaaggtagta gatttgcctg ttccaat
//
LOCUS       DACDNO010000220         1717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.174, whole genome shotgun sequence.
ACCESSION   DACDNO010000220 DACDNO010000000
VERSION     DACDNO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.174"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..1610
                     /gene="msr"
                     /locus_tag="IWI22_002990"
     CDS             132..1610
                     /gene="msr"
                     /locus_tag="IWI22_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9170280.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1657..>1717
                     /locus_tag="IWI22_002991"
     CDS             1657..>1717
                     /locus_tag="IWI22_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9170281.1"
                     /translation="MPTNKKEGIIFTTMMCFLMV"
BASE COUNT          601 a          284 c          363 g          469 t
ORIGIN      
        1 ttggtgattt agtcatagtt atttcgttca gagtaggaaa agatgtgatg cagttttttt
       61 gcaaacagat ttttctgctc ttttttagta tcgataattg ttgttttaca tgaaatagga
      121 gtgtaaaaca tatggaaaat ttagcagtaa atataacaaa tctacaagtc agctttggca
      181 accagctaga actatctatt gattctcttc gtgtctatca gcaagatcgt atagggatca
      241 taggagaaaa tggtgtcggt aaatcgactt tgctcaaact aatagccggt gaactttttc
      301 ccgatcatgg gaaaatccaa acagagatca ccttcaacta cctgcctcaa ttaacctatc
      361 tcgctgaagc aaaggaccta aatttggaat tagccagtca tttccagtta agactggaag
      421 aaacttcaga gcggaaatgg agcggagggg aagaacgaaa gatcgagttg atacgtcttc
      481 tttcttctta tgaacaaggg atgcttctag acgagccaac aacccatcta gatagaaaaa
      541 gtattgatcg actgattgaa gagcttcgtt attattatgg tacgctggtt tttgttagtc
      601 atgatcgcta ttttctagat gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa
      661 tccgagagtt ttcggggaat tatagtgcct atctcactca aaaggaattg gagaaaaaga
      721 ctcagctacg agaagcagag tcgatcatga aagagaaaaa acgattggaa aaatcgatcc
      781 aagaaaagaa aaaacaagcg gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac
      841 aaatcagacc ggatcggttg tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc
      901 aaaagaatgc gaaaacatta gagagaagac tccaaaaaat aggagaaaca accaagccgc
      961 agcagatgaa acaaatccgt tttccagtac caaaatctct tgaactccac agccgctatc
     1021 ccatcatggg acaaaatgtc caattggaaa gaagtgggag aacattactg gtaaatggtg
     1081 attttcagtt ttctttaggt aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga
     1141 caaccttatt agaacatatc cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa
     1201 gctttcaagt atatcagcaa aagggttatc aaatgacatc tgaagaatcc atcattcgtt
     1261 ttgtcatgag acaaacagag ttttcagaat cgcttgtccg tagtttgctg aatcacttag
     1321 ggtttgctca ggaaactttg acgaaaccgt tatgtacatt aagtggggga gaagcgaccc
     1381 gtctgacgat tgctttgctt tttactaagc caagtaatgt gttgctgtta gatgaaccga
     1441 ctaattttat tgatatggca acgatcgaag ctttagagaa gctaatgcaa atatatccgg
     1501 gaacgatttt gtttacttct catgattctt actttgtcga gcgtacggct gatgaagttt
     1561 atgaaataaa agggcagaaa ataaaaaaag tacttacgag aaatttttaa atgtgctacg
     1621 atgattttcg tgacaaattt aaaggagtgg tacagattgc ctacaaataa aaaagaagga
     1681 ataattttta caacaatgat gtgttttttg atggtct
//
LOCUS       DACDNO010000221         1465 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.177, whole genome shotgun sequence.
ACCESSION   DACDNO010000221 DACDNO010000000
VERSION     DACDNO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.177"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            7..372
                     /locus_tag="IWI22_002992"
     CDS             7..372
                     /locus_tag="IWI22_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9170282.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            373..>1465
                     /gene="aac(6')-Ie"
                     /locus_tag="IWI22_002993"
     CDS             373..>1465
                     /gene="aac(6')-Ie"
                     /locus_tag="IWI22_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP9170283.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVF"
BASE COUNT          606 a          118 c          245 g          496 t
ORIGIN      
        1 gataatatgg tttttgatat tgataattta aaaggatttc ttaatgatac cagttcattt
       61 gggtttatag ctaaagaaaa taataaaatt ataggatttg catattgcta tacactttta
      121 agacctgatg gaaaaacaat gttttattta cactcaatag gaatgttacc taactatcaa
      181 gacaaaggtt atggttcaaa attattatct tttattaagg aatattctaa agagattggt
      241 tgttctgaaa tgtttttaat aactgataaa ggtaatccta gagcttgcca tgtatatgaa
      301 aaattaggtg gtaaaaatga ttataaagat gaaatagtat atgtatatga ttatgaaaaa
      361 ggtgataaat aaatgaatat agttgaaaat gaaatatgta taagaacttt aatagatgat
      421 gattttcctt tgatgttaaa atggttaact gatgaaagag tattagaatt ttatggtggt
      481 agagataaaa aatatacatt agaatcatta aaaaaacatt atacagagcc ttgggaagat
      541 gaagttttta gagtaattat tgaatataac aatgttccta ttggatatgg acaaatatat
      601 aaaatgtatg atgagttata tactgattat cattatccaa aaactgatga gatagtctat
      661 ggtatggatc aatttatagg agagccaaat tattggagta aaggaattgg tacaagatat
      721 attaaattga tttttgaatt tttgaaaaaa gaaagaaatg ctaatgcagt tattttagac
      781 cctcataaaa ataatccaag agcaataagg gcataccaaa aatctggttt tagaattatt
      841 gaagatttgc cagaacatga attacacgag ggcaaaaaag aagattgtta tttaatggaa
      901 tatagatatg atgataatgc cacaaatgtt aaggcaatga aatatttaat tgagcattac
      961 tttgataatt tcaaagtaga tagtattgaa ataatcggta gtggttatga tagtgtggca
     1021 tatttagtta ataatgaata catttttaaa acaaaattta gtactaataa gaaaaaaggt
     1081 tatgcaaaag aaaaagcaat atataatttt ttaaatacaa atttagaaac taatgtaaaa
     1141 attcctaata ttgaatattc gtatattagt gatgaattat ctatactagg ttataaagaa
     1201 attaaaggaa cttttttaac accagaaatt tattctacta tgtcagaaga agaacaaaat
     1261 ttgttaaaac gagatattgc cagtttttta agacaaatgc acggtttaga ttatacagat
     1321 attagtgaat gtactattga taataaacaa aatgtattag aagagtatat attgttgcgt
     1381 gaaactattt ataatgattt aactgatata gaaaaagatt atatagaaag ttttatggaa
     1441 agactaaatg caacaacagt ttttg
//
LOCUS       DACDNO010000222         1398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.181, whole genome shotgun sequence.
ACCESSION   DACDNO010000222 DACDNO010000000
VERSION     DACDNO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.181"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1279
                     /locus_tag="IWI22_002994"
                     /note="aph(2'')-Ia"
     CDS             <1..1279
                     /locus_tag="IWI22_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP9170284.1"
                     /translation="EDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGM
                     DQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRII
                     EDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDS
                     VAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSIL
                     GYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLE
                     EYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRL
                     TGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEE
                     YYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          595 a          106 c          238 g          459 t
ORIGIN      
        1 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
       61 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      121 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      181 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      241 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      301 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      361 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
      421 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
      481 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
      541 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
      601 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
      661 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
      721 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
      781 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
      841 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
      901 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
      961 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1021 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1081 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1141 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1201 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1261 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1321 taaatagttt aaaaaaatga aaaataataa aggaagtgag tcaagtccag actcctgtgt
     1381 aaaatgctat acaatgtt
//
LOCUS       DACDNO010000223         1396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.182, whole genome shotgun sequence.
ACCESSION   DACDNO010000223 DACDNO010000000
VERSION     DACDNO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.182"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..732
                     /locus_tag="IWI22_002995"
     CDS             <1..732
                     /locus_tag="IWI22_002995"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9170285.1"
                     /translation="LLQNKNHIWDEWAFERYIKSPDYNGPDMTDFGHRCLTDPEFNEE
                     YKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFIDQIKQVIEAIKKTP
                     DSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRSGDVFLGVPFNIASY
                     ALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIRAFPTLVLNQEKQSV
                     FDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            752..1279
                     /locus_tag="IWI22_002996"
     CDS             752..1279
                     /locus_tag="IWI22_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9170286.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
BASE COUNT          453 a          253 c          305 g          385 t
ORIGIN      
        1 ctattacaaa ataaaaacca tatttgggat gaatgggcat ttgaacgcta catcaaaagt
       61 ccagactata atggaccaga tatgactgat tttggccata gatgtttgac agatcctgaa
      121 ttcaatgaag aatataaaaa gcagagcaag cttttttgcg agaagatcct aaccgatgat
      181 tcctttgctg aaacatacgg cgatcttgga cacatctacg gctatcaatg gcgtcactgg
      241 gaaacaaaag acggtggctt tatcgatcaa atcaaacaag tgatcgaagc aatcaaaaag
      301 actcctgatt ctagaagatt gatcgtttct gcatggaatc cggaagatgt tcctagcatg
      361 gctttgcctc cttgtcatac gatgtttcaa ttttatgttc aagagggacg gctaagctgc
      421 caattgtatc aacgcagtgg ggatgtgttt ctaggcgttc cgttcaacat tgcaagttat
      481 gcgttgctga ctcacctaat tgcacatgaa acaggtttag cggttggcga atttgttcac
      541 acattaggag atgctcatct ttatatcaat catatcgaac aaatgaaaga acaattgagc
      601 cgagaaatac gcgcatttcc gactttagtt ttgaatcagg aaaagcagtc agtatttgat
      661 tttgagatgg aagatatcca attagaagga tatgacccac atccggcgat caaagctcct
      721 atcgctgttt agaatcagaa aggatgagaa aatgttagct gcaatttggg cacaggatga
      781 aaacggattg attggaaaag aagaccgtct tccttggcgt ttgccaaatg atctgaaatt
      841 cttcaaacaa atgacagaag caaatacact agtgatggga cgaaaaacat tcgaagggat
      901 gggtagccgt ccgttgccga atcgtcagac gattgttttg acgagagatt cttcctataa
      961 agcagaaggc gtgcatgtca tgcatagtgt ggaagacgtg cttgctctgg aaaaagaaac
     1021 agacggtatc tttttcatcg ctggagggtc agctgtttat caagaatttc tgccattttg
     1081 tacgatatta taccgtacag tcatccatca tgtattcgat ggagatgctt attttccacc
     1141 tgttgactgg tctgattggt cactgatcaa tactagtcaa ggagaaatgg atgaaaaaaa
     1201 tcgttatccg catcaatttg aaacgtatca gcgtaatgag aatcctgtaa aaagcgggac
     1261 aggcgaattg acgatataaa aaagctatgg atagactagc tgttatttag agctgtccca
     1321 tccatagctt tatttttttt attaagtaaa gaataaaaca gagaagtaca taaaacctgc
     1381 acctaacata acaaac
//
LOCUS       DACDNO010000224         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.195, whole genome shotgun sequence.
ACCESSION   DACDNO010000224 DACDNO010000000
VERSION     DACDNO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.195"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..164)
                     /locus_tag="IWI22_002997"
     CDS             complement(<1..164)
                     /locus_tag="IWI22_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9170287.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IWI22_002998"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IWI22_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9170288.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          397 a          128 c          195 g          353 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gtt
//
LOCUS       DACDNO010000225         1032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E41205_2015
            SAMN12639680-rid9884353.guided.197, whole genome shotgun sequence.
ACCESSION   DACDNO010000225 DACDNO010000000
VERSION     DACDNO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639680
            Sequence Read Archive: SRR10030911
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742216.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742216.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 03:56:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,000
            CDSs (total)                      :: 2,933
            Genes (coding)                    :: 2,862
            CDSs (with protein)               :: 2,862
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 5, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 5, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 30 of 71
            Pseudo Genes (incomplete)         :: 31 of 71
            Pseudo Genes (internal stop)      :: 15 of 71
            Pseudo Genes (multiple problems)  :: 4 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639680-rid9884353.guided.197"
                     /isolate="E41205_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            87..146
                     /gene="ermCL"
                     /locus_tag="IWI22_002999"
     CDS             87..146
                     /gene="ermCL"
                     /locus_tag="IWI22_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAP9170289.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            194..928
                     /locus_tag="IWI22_003000"
     CDS             194..928
                     /locus_tag="IWI22_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAP9170290.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          415 a          136 c          140 g          341 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca gcttgtgcta taattattct
       61 aatttcataa aaaggagaat taatatatgg gcatttttag tatttttgta atcaacacag
      121 ttcattatca accaaacaaa aaataagtgg ttataatgga ttgttaatat aaacctcata
      181 ggagtgggtt ataatgaaca aaaaaaatat aaaagatagt caaaacttta ttacttcaaa
      241 gcatcatata aatgaaattt tgagaaatgt acatttaaat acaaatgata atattattga
      301 gattggttca gggaaaggtc atttcacgtt tgaattagct aaaaggtgta attatgtaac
      361 cgccattgaa atagatccta aattatgtag gataactaaa aacaaactta ttgaatatga
      421 gaacttccag gttatcaata aagatatttt acaatttaag tttcctaaaa ataagtcata
      481 taagatatat ggaaatatac cctacaatat aagtacagat ataattcgaa aaattgtttt
      541 tgaaagcaca gctacagaaa gttatttaat agtggaatat ggatttgcta aaaggttgct
      601 aaatacaaat cgttcactag cactattttt aatgacagaa gttgatatat ccatattaag
      661 taaaatccct agagaatact ttcatccaaa acctagagtt aatagctcgt taattgtatt
      721 aaaaagacac ccttcaaaaa tatcactcaa agatagaaaa caatatgaaa attttgttat
      781 gaaatgggtt aacaaagaat acataaaact attttccaaa aaccaatttt atcaagcctt
      841 aaaatatgca agaattgacg atttaaacaa tattagcttt gaacaattct tgtctctttt
      901 caatagctat aaattattta atagataagt taaggggtgc ataaactgca tcccttaact
      961 tatcttattt tgcttaaaca atcttggccc gtaaactgca taactcatat gtaaatcgct
     1021 cctttttagg tg
//
